LOCUS       DAOPFL010000001       101971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_171_length_101971_cov_105.292564, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000001 DAOPFL010000000
VERSION     DAOPFL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 101971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 101971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101971
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171_length_101971_cov_105.292564"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1595)
                     /locus_tag="PLG69_00005"
     CDS             complement(39..1595)
                     /locus_tag="PLG69_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPI41921.1"
                     /translation="MSKKLENNSTSIGRIINLLYRYLMVASFITIIAVDLHSELNANN
                     IVREPNSQLNVFQEDFNYFYRILLDSHPGIETDNTSFNQKVEAFMECLATVTDTLQFE
                     ARLRAFTHDLHDGHTRVSSFSNQGSPIYPIGLWWHESGWYVEMISTEFKDLIGSKVIS
                     INGIDAETFMTKLMQLTTGENVYWFRDQVSFFLRNSANLHALNLDRPDGVLQLIIKQN
                     GTFSEVNLYEVERPSLWGNQKTGITALHDDNYWGQALPEDNVYYIQFNAMSDPVGTSD
                     TPFSGWISFLKESFAVIDSLGIDNLVIDLRNNSGGNSTMGDILLSFCSLPDSIYYFGG
                     KMKISDLVLNHYQMNLTSMNKALSSDLGYEVLETELPFTVIFYDGKSPFLRPRTLSEI
                     VPNAMSNDSIKKYNGKLILLTGSQTYSSAVMFATLCQDNGLAIVYGSPTGGKPSSYGE
                     GLNFRLPNTGIMCSVSVKYFQRPDTSRDPSDSLYPDVDVLITPEEYFSPIDTLWERVL
                     NDIRNSDLYK"
     assembly_gap    1830..1929
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2133..5027)
                     /locus_tag="PLG69_00010"
     CDS             complement(2133..5027)
                     /locus_tag="PLG69_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPI41922.1"
                     /translation="MKKFFLLIFALLSLSILCAVPQYLIGLPVSELSLDKEDKTLRRA
                     VQDLAETDLEIYYYNDSYVIAGTDRNNYPGAINLGTPKSGKFYLITIGERYSKFDFST
                     VGEVLLEMGSVVLIKTDRDEIQLRQKIQNPFVPLLLEPMRFSETLSWHTAISSTRNEI
                     ENLINTVSEDSVLYFLQCLQNFSTRYALANNHLTVANWIKSQFQRFGINNSVLHSFQW
                     YGTMQYNVVATITGTVYPDTYIIVGGHHDSITNDNPMIFAPGADDNASGTVAALEMAR
                     VMMATNYQPKCSIRFVTFAAEEVGLCGSSNYANMADQTNMNIRVMINHDMIANTSPNF
                     WDPRVLLMPYDGFISHTDYAAMITSQYTTLQPVYGYLNSSSSDSHSFWQHGFPVVYFF
                     EYNFSSVYHSNDDTVTNLDPVYCASVIRASTAVTASFANMPSPPANLTAQDEGTGNSI
                     HLSWNPVPDPDFSHFHISWGPSQNNLIYSQTTTNNEISITGLNTDQLYFFAVSSVDFS
                     GFESYSVATQCIPRLIPQTPVSFIDQPLPEIVKLSWQSNTELDLAGYKIYRSLNSSET
                     GALIATLPPTLCSYSDSNVQGGEFYYYYRLCAFDNDNNSSPFTPVLKTRPLSMNCGLL
                     IIDETADFSGSSPFQPTDEAVDNFYDLLIDGLWNTAHFDLADFTETLRLCDIGIYSVI
                     IWHGNDFSNMIYPNQVKDVLKNYIYYGGKVLFSVFHPGLAFELNADYPTVFSADSFIN
                     EVLGISGTDYTIQARFNKAESVYSGYPDINVDPNKVPASFNGHLYQVEALTVNSQATP
                     IYHYASAYDNNSPQGILNGGVVGVQNTYFSGKTVTFSFPLYFMDFNQSRQVVHYIIEN
                     YFGFPVAVGDEVNIPVAEISILPNQPNPFSVSTQINILCKNSNAPLLVQVFNLKGQLI
                     KTLFQGKPEGKNTLSWEGNDNNGKKVSSGIYFLKAQQGNKVQTRKILLLK"
     gene            5457..6416
                     /locus_tag="PLG69_00015"
     CDS             5457..6416
                     /locus_tag="PLG69_00015"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPI41923.1"
                     /translation="MKILITGGAGFIGSHLAERLLQEGNEVYVIDNLSTGRLENIESF
                     KDKPNFHLNIGSVLNRELLDKLISNVEQVYHLAAAVGVKYIIENPLLSLKTNIMGTDN
                     VLEFCNKYKAKALIASTSEIYGKNEQIPFSETDDRLLGSTHISRWGYGCSKAIDEFLA
                     LAYFREKKLPVVIVRCFNTVGPRQTGQYGMVLPKFIKAALLNQPLVIYGTGEQTRCFA
                     DVSDVVDAFIKLMNTPECAGEIFNVGTTESISITDLAQKVKDMCHSKSRIEYMRYEDA
                     FEEGFEDMMHRQPDLTKIKNFIGWEPKHKLDHIISRIIDYYEK"
     gene            6406..7794
                     /locus_tag="PLG69_00020"
     CDS             6406..7794
                     /locus_tag="PLG69_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPI41924.1"
                     /translation="MKSRFLLILLMSLSLLSALTPTPTGDIIPISVSLTGFVENPGVY
                     QMTPVNRLSDLLLLNKTATLERIEKTQVLREKSVEVPKPAELLSPPTPEKEKEEIITL
                     ENNQGLRRIKITRAGKIETYDLLKFYRLGDISQNPLLKDGDVVFVPAIKDFISIGGGI
                     NLPGELEFVEGDKLGTIIDLSLGFTFDADISKVQLYRYKENRIDYDVLNYDLKANPAF
                     WDLPLKADDRILISCDAEIRTRQRIKIYGQVKNPGEYVIDANTTLYDVLQQAGGLTKR
                     GDIKSMVYYNENINADPDPFLEMLMQRTMSEMTPLEYSYLRNNLMQLKGKYSIDPVKM
                     INSEGKEANPYLLDGDRIYIPEKIDMVWVSGQVKNPGMVPWLEGKDWNYYIQAAGGYT
                     NNRKTGKGRIIRANSGNWVKPGKNVVIRAGDTVFVPAQTDRSMWTDVKDIVTLTSSVV
                     TIILGIRTFTRN"
     gene            7805..8911
                     /locus_tag="PLG69_00025"
     CDS             7805..8911
                     /locus_tag="PLG69_00025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPI41925.1"
                     /translation="MKVPMLDLQAQYEPLMPKIKEALERVFAEHNYIMGPQVKEFEEK
                     MANYLGIKNAIGCASGTDALVLAVKALGIGAGDEVITTPFSFFATASSIWRNNATPVF
                     VDIDPLTFNLDPGNIEKAITEKTKAILPVHLFGQCANMDSIMAIAHKYNLYVIEDNAQ
                     GIGCTWDGKMSCSFGDIGILSFFPSKNLGAMGDAGMCLTNNDDYAFKLRQLRVHGENP
                     KYYHQWVGLNSRLDTLQAAVLSVKLDFLEGWSKGRRANAEFYNEHLKNVPGIRIPYID
                     PKAVSIYNQYTLVCEKRDELMAYLKEKEIGCAVYYPLPLHLQECFSSLGYKKGDLPVA
                     EEMAEKVLSIPIYPELTREQKEYVCKTITNFYCH"
     gene            8925..9710
                     /locus_tag="PLG69_00030"
     CDS             8925..9710
                     /locus_tag="PLG69_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPI41926.1"
                     /translation="MHFIVCIKQVPGTTEIKIDPNTNTLIREGVESILNPFDAYAIEE
                     AVRLKEKYGGTVTAISMGPNQCETTLREAVSLGVDNIILLSDRRFAGADTYATSLTLA
                     AAIRKIGDYTLILTGQQAIDGDTGQVGPGIAAHLNIPQTCFVRKIESFNEQQAVVERL
                     LEDGFDRVSMQLPALISVVKEINVPRLPSLRGKRNAKTVPLTVWNCDDLGLDEKETGL
                     NGSPTQVLNIFSPHHEKQVEKFEGNSDEAVELIVQRLSELTNR"
     gene            9707..10534
                     /gene="lgt"
                     /locus_tag="PLG69_00035"
     CDS             9707..10534
                     /gene="lgt"
                     /locus_tag="PLG69_00035"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPI41927.1"
                     /translation="MIKYPEINPDIVSFNLGGLNLHIRWYGFFYVLSFILAFILYKPF
                     LKKRNIKLTKEQYESIIFYVMLGVILGGRLGYVLFYNLGYYISHPLLIFAVWEGGMSF
                     HGGALGVIIAGLIYCHKHKLNFYALADAAIPIVAIGLGLGRLGNFINGELWGKPTDLP
                     WGMIFPGADNLPRHPTQLYELFLEGIVMFIVTYYLLKKLKKEGLVFWSFIGLYGVFRF
                     LIEFVREPDDLDFYTRFGYIFGFMSIGQFLSLIMIIVAIWGFYKLNAPHKKEKKPKI"
     gene            10680..11417
                     /locus_tag="PLG69_00040"
     CDS             10680..11417
                     /locus_tag="PLG69_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41928.1"
                     /translation="MKKALISVLIISAIIISACAGPKDKSEEEKLRQQLKKWESFDSE
                     GIAEVSYKSLAIRKMFSAAKNGSELRFDLFDGGLLGSAGEPLLTMYLRDYVALKSPFI
                     PMLELLDLTPSAPLQSLKLSANADSLVALYGERIIRDKKLELNGVTINFNKDYLLDSV
                     WEPENKTQMKALYNSKGDLSELVVTSIDNISLRMSFDKIEYVQPQIIPLPKPELSFSE
                     DALKGLNNLNLESLLKDFLQYKKERQK"
     gene            11414..12709
                     /gene="purB"
                     /locus_tag="PLG69_00045"
     CDS             11414..12709
                     /gene="purB"
                     /locus_tag="PLG69_00045"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPI41929.1"
                     /translation="MIPRYSKPEMERIWTLENRYECWLEVELAAARAMFEKGIIPKED
                     WETINTKADFDTNRIDELEQITKHDVIAFLTNVGENIGPASRWLHFGMTSSDVLDTAT
                     GMQLKSSGELILTEISRLAEILKLKARQYKNTICMGRSHGIHAEPTSFGLKFALWYEE
                     MQRNIKRLNEAIEEISVGKFSGAVGNFAHLSPEIEELACKYLDLHPANVSTQIIQRDR
                     YAFFLSVLALIASGIEKIALEIRHLQRTEVHEVEENFSKGQKGSSAMPHKRNPIVSEQ
                     LCGLARILRSNALSAIENNALWHERDISHSSVERIILPDSCILIHYMLDRTCSLIENL
                     IVYPENMQKNLELTKGLVFSQALLLHLVQSGLTRENAYSFVQQEAMKSWETDSSFLDN
                     VLANKEITSVLAESEIRDIFSYERYLRNVDYIYRRCGIL"
     gene            12725..13174
                     /locus_tag="PLG69_00050"
     CDS             12725..13174
                     /locus_tag="PLG69_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPI41930.1"
                     /translation="MKKYLIIGLAVVLCLPAFLFCKEETNRLIGYWLTGDKTMKIEIY
                     DNGDSTFAGKIIWLKEPNDKNGKPKMDTKNPDEKLRTRPLLEMVILTGLIPESNTKYK
                     SGKLYDPNSGKTYSATIELVNNNTINIRYFIATPTLGRTDTWTRTSR"
     gene            complement(13645..15528)
                     /locus_tag="PLG69_00055"
     CDS             complement(13645..15528)
                     /locus_tag="PLG69_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI41931.1"
                     /translation="MKKMVLFLIVVIIILPLSAVYHKIGEYDTPGSANSIFVSNNIVY
                     VADGESGLQIIDVSNLQNPALLGYYDTLGYQYSVFVSNNIAYIADYYGGLRIIDVSNP
                     QNPALFGYYGTLGSARYVTVSNNIAYIANDYSGLQIIDVSNPQYPSLLGSYDTPGHAE
                     SVTVSNNIAYVADCDTGLQIINVSNPENPALVCSYDTPGSAESIFVSNNIAYVADYVA
                     GLQIINVSNPQNPALVGYYDTPYYAYSVFVSNNIAYIADDDSGLQIIDVSNPQNLSLL
                     GYYNTPGYAYSVFVSNNIAYIADYWAGLQIIDVSNPQNPSLLCSYVTPSFASSIFVSN
                     NIAYVADGNSGLQIIDVSNLHNPVLLGSYNTPGPALFVTISNNIAYVADYYEGLLIID
                     VSNPHNPYLLGSYDTPTNSYSVFVSNDIAYIADGNSGLQIIDVSNPQNPILLNTILPR
                     PSSYIYKCIIFNNLLIISDTNWDELYFYNISNPAAPEFVCRYSWNLSTEDMVIQNGYL
                     YTANGLYGLNILDLNQVMEAEDSIQLPPITFNLKNYPNPFNPETTISFELSKPGNVDL
                     NIYNLKGELVKRLINNKMSNGKHSMVWDGKDNNGKICSSGVYCYKIESNGRVETKKML
                     LMK"
     gene            16650..17303
                     /locus_tag="PLG69_00060"
     CDS             16650..17303
                     /locus_tag="PLG69_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41932.1"
                     /translation="MKRAYLIIPFAIILLLLTSCATTGEEQATKLVTKYDIALTKVSR
                     GGDETTRSKAPVADTTLVDKLRYHYDDEFMHTIWYASENGFELTLYNASDKNIILDWE
                     KGLYLDYDNIGHRLLISSTPDSEKDKPQSPSTIPPRSNITEIIYSADHTNPSTLLGVY
                     VRTPLLPTEYKKAVRFNGKEMKIQLPVRIDNTTYNYEFTFKIVGVRQEQAKSNLLFG"
     gene            complement(17415..17624)
                     /locus_tag="PLG69_00065"
     CDS             complement(17415..17624)
                     /locus_tag="PLG69_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41933.1"
                     /translation="MVSSVYPIKRYEFSLHKEFAGSVEVILAEMAPEIFYFVCYLTIR
                     EENTVSYLGALLTHCLPITDGISIG"
     gene            complement(17729..18166)
                     /locus_tag="PLG69_00070"
     CDS             complement(17729..18166)
                     /locus_tag="PLG69_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPI41934.1"
                     /translation="MKIVPYRKTNELRPVSNMLSLFDEFFNRFYEEEGEEDNFRAMAI
                     DIVEHDKDFEILANLPGFKKDDVKISIHDNQLMIEANSNVTKEETKGTVYRCERYSGS
                     YRRNLLLPENVEVSKISAKMEDGVLKVIIPKKEPSPKKEITIE"
     gene            18686..20485
                     /gene="lepA"
                     /locus_tag="PLG69_00075"
     CDS             18686..20485
                     /gene="lepA"
                     /locus_tag="PLG69_00075"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPI41935.1"
                     /translation="MKEEFIRNFCIVAHIDHGKSTLADRFLEETHIIGKGTEVSQILD
                     SMDLEREKGITIKSHAVRMVHNYQGQDYVLNLIDTPGHVDFSYEVSRALASCEGAILL
                     VDASQGIEAQTMSNLYLALDNNLEILPVINKIDLPKADIEGTENDLCEILGCLPEEII
                     KVSAKTGEGVKDLLDAIVTRLPAPKGNPDTAPRALIFDSYFDMYRGVVVLVRVFEGKL
                     QKGDKIKLFSTAREYEIEEIGYLGLKFSPQTELTCGEAGYIIANIKEVADARVGDTIT
                     LSKGGCENPLPGFREPKPMVYSSIFPINGEDYENLVESIAKLKLNDASLIYEKESSAA
                     LGYGFRCGFLGMLHLEIVKERLLREYNIPIMATTPSVRFLIKLKSGEELQINNPVDFP
                     DPSTIESIMEPFMDTEIIVPTDYIGNVLKLVQERRGIQKDIQYIDEKRVTLHYELPLI
                     EIIFDFYDKLKTVSRGYASLDYTFKDYRETDVVKVDILINGEKVDAMSFICHQDKAYN
                     WGKSVTETLAEVIPKHLFKIALQAAIGGKIIARSTINPLRKDVLAKCYGGDVSRKRKL
                     LEKQKEGKKKMKEIGSVSVPQEAFLAVLKADRE"
     gene            20489..22165
                     /locus_tag="PLG69_00080"
     CDS             20489..22165
                     /locus_tag="PLG69_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPI41936.1"
                     /translation="MVFFQSRHCFLTLFFCLSFGLILAINIGNISFQGISGIEETELF
                     KASGLQKGQEYNPELISNATSNLYKYLQNKGRYFVWISSPELIPEDEGTVSLLFKMQE
                     KAPSDEVKIRFQGMQYFSEAKLFQLLLLSPERNYHLNQLSRIMQQTLSLYQSRGYLFT
                     KVELDSLVWEEALSAYIGIDEGKPLRVKNYIFKGNKVTKDKTLLHLSGLENIDLITPE
                     VLTQAEENIQRKSYIRNCNVEPVNENTLLINIEEGKMTYLEGVLGMNRINEELKLSGQ
                     IRLQFLNLWGTDRAIKLFWKQIPTSNSELSLAYHESGIPGIPIAGDIELYRAQQDSTW
                     IKTRGSLEIYYQMLKHSLGIELATESLNPGARRPAIINKEKAQSIGAFWNYSNYAGGV
                     NPYKGMEMKFLYRLTGSNLTKHLYGATEAGVKGYIPVKNRFVGFIGAEIRNLENKNAE
                     LWQQYKMGGYGTLRGYYEDEFSSFRLAWINYELRYRLNPDSRIYLLFDQGFLGKEKNK
                     LKTDLFGIGFGMKINTRLGILGLEYALGYRDKHFANLGSGMIHLGLDVAF"
     gene            22382..22570
                     /locus_tag="PLG69_00085"
     CDS             22382..22570
                     /locus_tag="PLG69_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41937.1"
                     /translation="MKKVLLITLLAMLLISTLVTGCKKKAEEPQEEVAPIEETTPAPD
                     TTVAPTEGQTPTTTPTGK"
     gene            23148..23870
                     /locus_tag="PLG69_00090"
     CDS             23148..23870
                     /locus_tag="PLG69_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPI41938.1"
                     /translation="MIGERLKQIRQVLGIKQVDLAKVLKINPSAISQMESGRTNPSLE
                     TLSELVVNYNVNLHWLITGIGKMFNTANDTSSQNGSWDNFQKLLNDRLEEIVQARLDL
                     MDSDTVEIPVSGEIAAGEPMENYGTLLDVVTVRRSLINGSLNNFVALRVNGRSMEPDI
                     RNQDVVLIRYCNEWRELAGKICAVRIDGGITLKRLILDDVQKMIVLLSINENYQPILI
                     DPDSHTDVTLIGSLYFLFRILP"
     gene            23885..25264
                     /gene="rmuC"
                     /locus_tag="PLG69_00095"
     CDS             23885..25264
                     /gene="rmuC"
                     /locus_tag="PLG69_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPI41939.1"
                     /translation="MTLVYLLIIVAIVFAIVNLYILLSQRHKGKENGNISELKGAFET
                     FERYNREEMSSLRTELLSISAENRKELSDSIKAQNEVLIKQSTASREELNNSFNTLKQ
                     QINNDSAVNREEQKKALNDLSENFTRRMTDLINIQQQEAETSRKTLETKMEQIRQNNE
                     TKLEQMRQTVDEKLHDTLEKRLGESFKQVSERLELVHKSMGEMQSLANGVGDLKKALT
                     NVKRQGVLGEIQLANLLEDILTPEQYEKNFRPNKNRDEKVEFAIRLPGSSEKEEFVYL
                     PIDAKFPIADYQNIMNAYDMGDLAEVENARKSMVQRIKNCAKDIRDKYINPPVTTDFG
                     MLFLPFEGLYAEVLRSPGLFETIMRDYHVIICGPTTTAAVINSLQVGFRTLAIQKKTS
                     EVWKILASIKLEFGKFGTILEQTQKKLQEASNNIEKASYRSRQIEKRLDKVQELPVEE
                     AEIEQILDV"
     gene            complement(25358..26026)
                     /locus_tag="PLG69_00100"
     CDS             complement(25358..26026)
                     /locus_tag="PLG69_00100"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPI41940.1"
                     /translation="MLCVNNNRAYIFTNHAPQDIVSRYQNIQRKDHLIAVDNGIKKID
                     ELGLEPFVLIGDLDSIEPEMLEKYAYIPFHKHPTEKNETDTELALDWCLKQQCYREII
                     ICNDMQGRFDHALAIAINLLKIKSPRGERISYPVPYRIESENQSIFILPDSIEFNNCQ
                     GKLLSLIPLSEKVVFSWSQNLKYHLNDLVIRDYQSRGISNIITASSAGIIKTEGICLA
                     VLEG"
     gene            complement(26013..26912)
                     /locus_tag="PLG69_00105"
     CDS             complement(26013..26912)
                     /locus_tag="PLG69_00105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPI41941.1"
                     /translation="MENEFINKGKMKISYQVGMDGVKHSFTLPHYAFILIIVLLAFLL
                     VSTIIMFTMAGSNSHRNEVLKRLETENKELRAKLDFYSATVDSIYKRLNELQIKINEN
                     SEDYPALNFKQGKKADFVFEPALRNQMENLETKLATILVLISEPVQAIPALPTGENPA
                     DYIPSIYPTFGRISDGWGLRVHPIRNEIEFHYGLDISNEAGTPIYATAAGTVVTTDYE
                     TSYGKRIIINHENGYQTLYGHLYSYMVRSGDQVIKGQIIGLMGSSGISTGPHLHYEVH
                     NNLGKVNPVAYLNRIDEPLYAMR"
     gene            complement(26979..27054)
                     /locus_tag="PLG69_00110"
     tRNA            complement(26979..27054)
                     /locus_tag="PLG69_00110"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27018..27020),aa:Glu,seq:ctc)
     gene            complement(27087..27161)
                     /locus_tag="PLG69_00115"
     tRNA            complement(27087..27161)
                     /locus_tag="PLG69_00115"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27126..27128),aa:Glu,seq:ttc)
     gene            complement(27168..27242)
                     /locus_tag="PLG69_00120"
     tRNA            complement(27168..27242)
                     /locus_tag="PLG69_00120"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27208..27210),aa:Lys,seq:ttt)
     gene            complement(29240..30328)
                     /gene="dprA"
                     /locus_tag="PLG69_00125"
     CDS             complement(29240..30328)
                     /gene="dprA"
                     /locus_tag="PLG69_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPI41942.1"
                     /translation="MERYKAWLCLKSAPLLGTKGTLELLQSFPDPQEFVGNPSHPLYQ
                     NGKLKPPTIKHLSEFTLPEDNKRILTLMEKHQLQYLCLTDPDYPAALKEIFDPPLILY
                     YQGDLTGTLKDLCIAVVGTRKPTSYGLEMCRKLLSPLCAKGVNVVSGLAMGIDTIAHQ
                     TALKAKAKTIAVFASGLDNIYPPMNKTLAENICENGALVSEYEPGSKLERWNFPTRNR
                     IISALANAVLVVEGSLNSGAMLTAKFAIEQNKPLYALPGNINNRNAQGPNLLIRQGAS
                     LISSANDLLMDLGLVPSPKEQMELIPELSSEEQTLYDILSSAQKELTFDEFMLKTGLG
                     FGKLSTLLLNLELKGLIAKSSGNSYIKL"
     gene            complement(30329..31339)
                     /gene="obgE"
                     /locus_tag="PLG69_00130"
     CDS             complement(30329..31339)
                     /gene="obgE"
                     /locus_tag="PLG69_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPI41943.1"
                     /translation="MFIDYARIKIKSGNGGDGTISFRREKYVPKGGPDGGDGGRGGDV
                     IAVGDSNLNTLLDYRYHKFFKAGNGKPGAGAKKTGSSGANCILRLPLGTEIYVLEENK
                     KYKLADITQAGEKVILSAGGSGGKGNYNFATPTNQAPRIATPGVKTEEMELELVLKLM
                     ADVGLVGFPNAGKSTLLSVLSSARPKIADYEFTTLEPMLGVVYISDYQNFVMADIPGI
                     IKGAHLGKGLGDQFLRHIQRTHLLLFLIDIATPDPLEAYRTLRSELYLYDSFMDKKPH
                     LIVISKTDTLSPEDLKQKLTEIRNAFKKEYNDEIFAISAVSKDGLDELKYKIYNILQQ
                     SR"
     gene            complement(31348..32826)
                     /locus_tag="PLG69_00135"
     CDS             complement(31348..32826)
                     /locus_tag="PLG69_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI41944.1"
                     /translation="MKHILTVILVGITTLLMAQYELPFGNIRHSSRDENGYIHLRWED
                     LSEGTLNPEFYYSANDAPWQNSTIYDISAGEKEALAPYCFNQKLRYRLHYSIEEMGET
                     IAMMHSAYWDSNSFPPSIDNLAYIGTDAEGDSVTVYSQNLDLRESYTAISSDKIYFTM
                     KNCSGSFPIMNSLTSYNVYLAVLGNITSLIDSVGYAMVYSFNIPGLISSGLYKVGYDS
                     FTQQPVFTRLGNVQSQVSGGALNLACNISDLTADPNFGALPDAIMMLGLTLKVDIDLA
                     SLEPQIGLGDYSTPAFIIFQDNYYQVSQNTLPVCTFNGYNPSTGILLIDYWDADADFP
                     LISEAILPDGTILQFLPTGLDYSFPVTYTLQLPVIEVDSLIWRFSDNGFDIVQDVVHF
                     VGNQDGYLIPSLLSCTMPNPVYSLPVNINLKGLCNCPVSIEIYNIKGQKVAEINQSPT
                     GNGELNINWKGMASGKQLSSGVYFMKVENAKQTLKRKFVVAK"
     gene            34211..36952
                     /locus_tag="PLG69_00140"
     CDS             34211..36952
                     /locus_tag="PLG69_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI41945.1"
                     /translation="MKKSLVVLLIVLAMSSFLFAAEYTIVDGSSNSYHIPVNGNSHYG
                     WSRFIFTATEMNTAGITGSFTIYSIAFKINNTVSDYTMDNQKVYFGYNYNSTLSGSTS
                     YPNPGGGSSFTKVYDGSITFNGPGWMEIILTTPFNFTWDGYAGLDIVWENWDGSRITS
                     GYPKFYYASKSNMAVYKESGTGTSFPASTGTTSNNHPSFKIITPPTELPNPAINPDPV
                     NGASGVVIDKELKWASGGGSPINYIVNLGTDNPPTNIAYNEVVTATKYTPANRFDYGT
                     IYYWQVIPHNIIGDAQDCPIWSFTTMDDPSIATFPYQESFDGTFPPNSDWLMRTGVLQ
                     DPITLSPTSIWEQDDWLNIPGTDKAARINIWGPLNAWLITPLFNIPDDSYYLSFDLAV
                     LKSDQTPTGTPPVYAPDDRIAVLIGDGYNWSTANIVREWNNTGSPYVYNDIRISGEKV
                     VIPLTGHSGHCRFAFYAGSTEYNADNDVMFNNMEVAAFSNLPLAAQNPIPENNAQDVP
                     LNAKLFWDAGGNAPVSYNLYLGQTLPGTPVECLSPHYTPAALAYGTTYSWKVVPVNPI
                     GEAIDCPVWNFTTTGTATVGAGTVTINDVPINPSVEISGLEGETVISASASFAPEGIG
                     LPNAGLVIQLSSSGVNLTGKHITINHSLGFIPSQIAYRILPSETYHIISNPGTWTAET
                     VSFILEAKADGDVDIVFPLDAESTLPVELSYFNAIYTAEGYVTISWVSQSETNHSGYN
                     ILRSEDRIFANAIRINPVLIDKGTELGTQIKYKFIDSEFAPNTVYYYWLESISLNGET
                     QYFGPVFVMTGENGSEPETPELPFITKLYNAYPNPFNPHTVISYSISKPATVTLEIYN
                     SKGQKIRSFINNHNSAGIYSMNWDGKDDTGKTVSSGVYIYRMKTDNFQQSKKMLLTK"
     gene            complement(37061..38041)
                     /locus_tag="PLG69_00145"
     CDS             complement(37061..38041)
                     /locus_tag="PLG69_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HPI41946.1"
                     /translation="MNKLKHHLIPLLIGLIFAIAIWQIAETLIADFWSKTCLSIFGSS
                     FVEMPTMDKNGIPMRYYPSAGKVYDPEFIAYESSRFYKTRIEEPRKNAFLLYSKWLEE
                     HLDENGNIPHNYDYPKAHLKKPWFSSASHSATMLALTERAGNLRSPETFIKARKMLYT
                     LQPEKGNFSFYEKKGGIWFQEFPTEPYSLSGMLKTLVNLSEYYKLVDDSLSWELFQQG
                     VIALQAKLPELAKKNLLDDKYHYKNKRSEHLELVALLEEVNSATPDSVFTPFIQNYQA
                     KSKQFVLFQFFSPFQPGRILGFLIGWAVLYLLAYLFFKPLRTGEHEGESS"
     gene            complement(38262..39047)
                     /locus_tag="PLG69_00150"
     CDS             complement(38262..39047)
                     /locus_tag="PLG69_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPI41947.1"
                     /translation="MFSFSFLVYAFLGALLSGISLAVFAPFVTLRRISYLGEALSHIA
                     FAGIALAILAGLNLTVTTLIFVMLIALGITLLAKKHNIQESNTITIFLSLSMALGIIL
                     ISLSKNYSFDLASYLFGNVLLITRNEIIALAVLSVLNIAFVCFFYKELFYLTYNPVMA
                     KVYRIKTDLVNLIFMLLLAANIVINVKSAGIILVTAQLILPAVIAFNLVHRLSKAIVI
                     SVLVAVFSAFIGFYFSFLLNLPTGACIVVFEAILYFLSLLFSV"
     gene            complement(39054..39782)
                     /locus_tag="PLG69_00155"
     CDS             complement(39054..39782)
                     /locus_tag="PLG69_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI41948.1"
                     /translation="MIQIKDLQYKINGKIILEDIFLELAEGEFAAIIGPNGAGKSTLV
                     KLILGLLELKEGSIFIDGVEHYNWLKNNTIGYLPQYEEFDNAFPATALDIVLMGVAGQ
                     LPLGTHFNKNHKQKAMEALEQTGVAHLAKELIGKLSGGEFQRVLLARALVSESKYLIL
                     DEPEASLDRPSVESFFALLKELNSKGKTIITISHDLTTLSKYCSFLVCLNRRLHCHNQ
                     MELIDADVIHKTFGDSLRIIEKDY"
     gene            complement(39791..40699)
                     /locus_tag="PLG69_00160"
     CDS             complement(39791..40699)
                     /locus_tag="PLG69_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HPI41949.1"
                     /translation="MKYLKWSVLITLLFAFSCQKQEEKQSPLLLASIHPYELILKQLA
                     GNEFEVKCIIPSNASPHTWSPGPADLKNFSSASFILANGLGLEANLEKNFAERKDVYV
                     EAATLLKDIIPLPERKLLAEEEVPEPVEHQHQHAQNPHLWTSPSLMIRLIGFLEKELS
                     TRFPNSSFVFKHNAQQMQQELKNLIDKIKTERAQYKNPAIITYHNAFHYFLQEFDIEE
                     LGFVQESPGKEPTPKELALLGNIIKEHNVQAIFLEPQMDRKAGETLSKEFNLKLLTLD
                     PLGSDGKAQTIAELIDSNWQKMKAGF"
     gene            complement(41001..42482)
                     /locus_tag="PLG69_00165"
     CDS             complement(41001..42482)
                     /locus_tag="PLG69_00165"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPI41950.1"
                     /translation="MKIDIIRKVPEDMDCIIIFQEENGALDKVELLPQNIAKTAQVYC
                     KSEDFKFAYGDLKNFAIVNGIQRQNIILLGAGNKNDLNTNKVRNLLASAIRFAAKLRA
                     KQIYLFQGFDCPITDVAFGHLLSETALLVTYQFNKYSSSAKNESEIEALHYMLLSKNT
                     RHLNRGIVEGRIYAETTNFARNLVNEPANVITPENLAETAKQAALQYGFSIDVHSLDK
                     LKRIKMDAFLAVGRGSTHEPKLIIMRHLGNPEHHQETIALIGKGITFDSGGYCIKSAQ
                     GMSNMKDDMGGAAAVIGTMCAIATMKLKVNVVGIIAACENMISGDAYHTGDVITSMSG
                     KTIEVVNTDAEGRLTLCDAINYALEKEHCDRIIDIATLTGAAVTALGTQISAVITNNE
                     PWLEQLKNAAHFTGENIWQLPAFEEYKEQLKSEIADLKNSGGPSAGTITAGLFLQEFV
                     QNKPWLHIDIAGTAFKDKEYGIYNYGATGAGVRLLTTLLKYME"
     gene            complement(42514..45534)
                     /locus_tag="PLG69_00170"
     CDS             complement(42514..45534)
                     /locus_tag="PLG69_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPI41951.1"
                     /translation="MNKKLYCLIILFVLCSSVFAYSFGQNKVNFAPQDFSTIQTMHFD
                     LYFPAGEDQFGRIAALMAEDIYYYIKAEFKVPILARIPIIFYSTKQEFLSTNITYYLL
                     TEGVGGFTESLHNRVVVPFEGSYANLEELLAHELTHAYINAMDNRFVNPNSMLRPTSF
                     PFWFSEGLPEYLSVGGEDDYNNMYLLDMVINNTIGRLNSADGYLAYRLGESFLTFIAE
                     TWGREKVPEYFFAIHSSFNLDEATKKVFGMDFEDLESRWHYQLKRDYYPLINSHDIPM
                     ENFEQRTFHKKDGSYFNLAPRFSPNGSRYVYYSNAGGRYSIWLAGTQGLAQPQLIFKG
                     EKSGKAEEFYYLRSALSWFPDNQRIAFSAKTATGDKIQILNVNTKKIVQTISIPELQA
                     IYEADVSPDGLFLVLAGQKNMQSDLYLYEIETGKLTQLTDDSYDDAHPRFSPDGKYIA
                     YDSEHKCEKQEIRYGLFSDLTRDIFTFNIETGEIVERTFENYDCFSPIWAEDGSKLVF
                     LSYKYNLANYEILDWETGQRAELTKCIAGIMNGDISFDDSYLVISNYFDGAWDIYFDD
                     NPLKNLIYEESSQPQSFVRADDLLQTIDLTRLDYYGKRTKAQHLSKPQSPQQNPRRPH
                     ISNNESDKSDSLKHFQDFSWDDKPDSINVPPKIEKYKTKFALDSIWGGLAYSSYTGTV
                     GAIDLTLSDLMGNYGIGISLGISGKIEDSNLFLSFMNLKHRADYGIGVYNFYDETLYR
                     RYLIGQDDYFRLRERELGMLFIYRYPFSRFTRLEFDSQLYYVKQEWDYLAPEDVPTEN
                     WQMDISKETDTVIAPGLAFVFDNALFGSTGPMVGWRYYYLIRKSFAKNELNYFTNYLD
                     LRCYNFFEKRYSLAFRLNAGISTGESPQRFNLDGLYGVRALNEDIDGERMILGSAELR
                     FPFFDYLYLAFPVPLQLRNIRGSIFADMGAAWDKNSDFRGVIDGKLNDIKLGYGFGPR
                     LNLGYFVLKFDIAWLTDLSKISKPMYYLSLSEDF"
     gene            complement(45543..46445)
                     /locus_tag="PLG69_00175"
     CDS             complement(45543..46445)
                     /locus_tag="PLG69_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPI41952.1"
                     /translation="MKKFYPNLRSVQIKTGIWTIVICLILVFSYFWLSGRLAVRSHYE
                     LKIAFPNVMGLEVGDKVMYRGMEVGRVKSITASKDKVITTANIRSDIILTEGTRFLVS
                     DSSLMGGKALNIIPGDGKNPLNIKKLQQGTSPEGMMELISKASAGLDELNETVKLLNS
                     PDGLLQSSRMLVQNADGTVTEMGNVAKEIKQELVITVNKIEDLTSSLQEVIAENKEPL
                     KNTLVEGNITIEKLSATLDSLKILSANLNRSAKALTENEGTAGLLLNDKQLYEKITNA
                     TDNLNALIKDIKENPKKYIKFSVF"
     gene            complement(46452..47747)
                     /locus_tag="PLG69_00180"
     CDS             complement(46452..47747)
                     /locus_tag="PLG69_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPI41953.1"
                     /translation="MFRKALVCAGGKRISVILLCLIVFSSFTIIPLSADILIPMDNTQ
                     TNHLKAYGIAFAGLKEHLVGRWLLNYRGGSFLFPDSESLTALCNIRGVRYENVSSSDV
                     AAILMEIQANNMESIALEKEPKIAVYIPPNALPWDDAVTMALDYAEIDYDRVWDDEVL
                     AGKLPEYDWLHLHHEDFTGQYGKFYGSYRNTSWYQNDVRVNEAMAAKHNFAKVWQLKH
                     AVAEKIRNYVINGGFMFAMCAATDTYDIALAAKDVDIVDAAIDGDGIDPQYQSKLDFS
                     RTFAFENFTLRPNPYEYEFSDIDASDYSSLRGPEADYFQLFDFSAKYDPVPTMLTQCH
                     TNVINGFLGQTTSFFKDKVKKSVIILAEVPGQNEVKYIHGNIGKGTFTFYGGHDPEDY
                     QHKVGDPETILELHKNSPGYRLILNNVLFPAAEKKQLKT"
     gene            complement(47975..48877)
                     /locus_tag="PLG69_00185"
     CDS             complement(47975..48877)
                     /locus_tag="PLG69_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPI41954.1"
                     /translation="MNPFIEKIIPLILAFFVGIAFKLLKLLSKDDAPVLLRVVLNVCL
                     PSLTIIAISNIQLKADMALIPFTAIIIVFLIYIISRTIGKHLKMEKTTFGSFLVGTMI
                     MNTAYALPFFFAAFGNEGLARASLFDIGNTFMIFTFTYYNAIKYGDNSKTAEIEWKKF
                     LRLPPLWAMLIAFSWKLTKVPMPALALNFLNFMGQPMVPLMMIALGLYFNPKRCNIGK
                     AAIAIFIRMGIGLGIGIVLSALFGFKGITRTVVTVCAALPIGFNTLIFANLENLDREF
                     AATIVSISIFIALFYIPWLIYVFQ"
     gene            complement(48874..50355)
                     /locus_tag="PLG69_00190"
     CDS             complement(48874..50355)
                     /locus_tag="PLG69_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPI41955.1"
                     /translation="MIFIYLLSGLFLGWSLGANDTGNIFGAAVETRMLKFKTAALIAS
                     IFIFLGAVFEGSGPSGTLGRLGSVDALGGAFTVALAAALTITAIVRTGIPVSTSQTIV
                     GAIIGWNFFSGRLTDFRNLLTIVSSWVIAFVLSGIIAALLFYLIRPVINRGKIHLLEQ
                     DVYVRLGLIIIGAFGAFSLGANNIANVVGVFVPVTPFKDINLGSFTLSGVQQLYILGA
                     ISIIVGIYTYSHKVMRTVGKDIFHLSPVTALIAVASEAIVLFIFASRGLQNLLLSIGL
                     PPIPLVPVSSTQIIVGAVVGIGLVKGGKNIRYNILGKVSLAWIAAPVIAFFFAFIALF
                     IVQNVFEQNVYQKTIYTFNHTTIRQIEKEGLDVNHLSSVNGRKFYREMVVYKELKAEK
                     YFSRSEILKIIRITEVYPLKVNTKLLQDKGLAIRFTEQQWEALVKLEGREFRHKWQLQ
                     ETLAKDPSWQISKEITERDKLHNQDLEEQFNLLFRTFYLPPEQ"
     gene            complement(50356..51000)
                     /locus_tag="PLG69_00195"
     CDS             complement(50356..51000)
                     /locus_tag="PLG69_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPI41956.1"
                     /translation="MALILKTTRFIETQIDAFLDILSDSATTFELAMEDYLNNRIEQF
                     ETRLAQIRENEHRADDLRVSIERFLYERTLIPENRGDVLAILENTDEVIDNIKDSLVQ
                     FSIEMPKIPECLNDLWMQTTKASSNAVEQLSVAVRSFFRDLAAVNNYIHKVYFFEREA
                     DHIGERLRREIFNLDIELSQKSQLRFFAIHIEKISDYAQVVCDRLSIYAIKRQL"
     gene            51680..52720
                     /locus_tag="PLG69_00200"
     CDS             51680..52720
                     /locus_tag="PLG69_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPI41957.1"
                     /translation="MKADTTNNTFSEIILYPVEEGKYEIALNKDSESIWLNLNQIAEL
                     YQTSKANISMHIHNILKDGELTRKATVKNYLTVQIEKGRQVHRNIRYYNLDMILAIGY
                     RVHSERGTQFRIWATQHLSEFLHKGFILDDERLKGNDRLTDYFDELLARIRDIRASEK
                     RAYLRVREIFALAIDYKPNSKAANKFFATMQNKMHYAATGMTAAEIIATRANAQVTNM
                     GLTNWKGNIVRKEDLSIAKNYLTSDEIDILNRIVNMFLEQAEFRTLRKQQIYTKDWEK
                     FLDKFLMDNELPILNGSGKITHNEAKELAENAYNEFELRRRKAIEDEAEAKYLADLTK
                     ATKLVSARRKKR"
     gene            complement(52925..53992)
                     /locus_tag="PLG69_00205"
     CDS             complement(52925..53992)
                     /locus_tag="PLG69_00205"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPI41958.1"
                     /translation="MPYFRSDLKDKKPSKISVPVKKRMMCLNESCLNPYQAIKQQFLA
                     KMEEIELNRYLSPVTEKLHLSLISYIGNSLTKENVLWGNGADDILYHIFLGVRENDQS
                     FAISLAPSYFDYKTFSAMVGLKMLFLDLDEDFSFSPEKYLELTSHPDCKLAILCNPNN
                     PTGNLFPDCQLLQIITSLPDKLVLVDETYFEFSGRTLADKLLQYPNLILVRSFSKAFS
                     SAGLRFGYAISSAENIYELSKVLTTFHTSILNQAFALTILENQDIFKAQVQQTIQLRE
                     ELFATLQTIEEIKVYPSATNFLTFSLAERTPEFFNHLLANDIAVRDVGSHPRLQNCLR
                     VTISCKEDVEAFVNALKQFLR"
     gene            complement(54243..54812)
                     /locus_tag="PLG69_00210"
     CDS             complement(54243..54812)
                     /locus_tag="PLG69_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPI41959.1"
                     /translation="MKKIIFLTLALIFVCGALLAQTDAPKDRSNPKVQSEGIKSPKPE
                     MPMQKCLEELKLTDAQKKKFEELKISFEKNKNTLNAEIKNLKIDLQKALKDENYKQAK
                     DLNKQIATKRNVLADAKVDFLAARMKELTPEQKATLKKNMPHFQGAMHKPTPCKDAKD
                     FRPQSRENCDECGECGSFGMPEKKSPPKQ"
     gene            complement(54809..55261)
                     /locus_tag="PLG69_00215"
     CDS             complement(54809..55261)
                     /locus_tag="PLG69_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41960.1"
                     /translation="MSKRWLIIILLISVSFNLAFIGSFIYLHWFHPHPQPPVRNEEMR
                     GSRPHFGPPPFERDEEIRNLRDKFETIKHSLMLELAKDPVDMAKVNALIDSSLVAQNN
                     LERRLAERMVAYRKTLTAEEAQEHFQRRAEFAKKRLHRDNIPKNRRNR"
     gene            complement(55254..55700)
                     /locus_tag="PLG69_00220"
     CDS             complement(55254..55700)
                     /locus_tag="PLG69_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPI41961.1"
                     /translation="MRCSKAKRYLEKELDGELDTRFQAKLQSHLDKCPACKVFLADAQ
                     KLHLMLSNLPKPEFPSWIHGQIMDKVHRLEAKNPGFFRRYKLAPVTAMLTIVLSLWAG
                     VKVGIVSYNNIIPEKTESITLTSVAYGSFGENTILDNIAENGEQGE"
     gene            complement(55746..56234)
                     /locus_tag="PLG69_00225"
     CDS             complement(55746..56234)
                     /locus_tag="PLG69_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPI41962.1"
                     /translation="MKKEHFEAFLRANEKRIYNYVLTLCANEQDALDIVQTVFIAVYK
                     NLDRIDEATALAYTYKIAHNKCLSFLKQKAHYINMEPERFNCIPEKTANANETDYTVL
                     KNAISALPPRLAAVIQLQYYEKMSYKEISATLGISIKAVESLLVRAKRTLRKKILQDK
                     ES"
     gene            56571..57827
                     /locus_tag="PLG69_00230"
     CDS             56571..57827
                     /locus_tag="PLG69_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPI41963.1"
                     /translation="MINKSVVFLARKYLSGRKTRGIGKAHYLCLIGITLGVLSLLCVT
                     SVMNGFRLDIRNRITGTFAEIRISAKEGETIKGYSAICNQLQDMKFSASPVIRNELLL
                     KYDSVVLPTLCFGIEPDRHKNVSSLLQKPDTGSKEMIQGLLVGNISPEEFSASGIALG
                     AGLASQLGVYLGDEIQVLSPMFNVPTAFGMIPRIRFLKVQAIFAAGMPEYDQNFSFMG
                     IDNAVFFASYSPGEADYIEVKPFSQMRKNQPLKTLKKAFPDFQIEDWSSFDPNLYSAI
                     RFEKFLMFVIMLFMFIIASFNLTGNLLKMISQKKRELGLLKALGLTDKDLQSLFLVQA
                     LFLCSAGIILGLTLGSLLLLIQKYTGIVKLSLGGGNSIILPVKFAFTDYLLITVVAYL
                     ITILSVLLPLKRLNKINAVELIKRAV"
     gene            complement(58297..58476)
                     /locus_tag="PLG69_00235"
     CDS             complement(58297..58476)
                     /locus_tag="PLG69_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41964.1"
                     /translation="MKNKRTELNLKEEKVLLTFIQQTKEGKYVKIDESSFPYSRIKEQ
                     SEETYFEDEYYYSIR"
     gene            complement(58492..59043)
                     /locus_tag="PLG69_00240"
     CDS             complement(58492..59043)
                     /locus_tag="PLG69_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41965.1"
                     /translation="MTETGSLGTIEAQMKLERAKKIAAELTNHSKWRSHGRSIKIKDL
                     EGIGIKIIRVDDNAKLAENIYRIQTVCRLLFESTSTFKIFATQDNKIFKQAVPVGAPR
                     ILPGKPTINVVEIEQECPKCGKKYNLYAKLVPNPKIDTDFKNKGFIPFPKVSKFICEC
                     GFEIDLLGIKNQIEIQTGKKIIV"
     gene            complement(59123..60247)
                     /locus_tag="PLG69_00245"
     CDS             complement(59123..60247)
                     /locus_tag="PLG69_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41966.1"
                     /translation="MKLNGKTIYAQSSDIKSRTYLEYRKDMKKKAIAELEILEWLEDK
                     IKELYPEKCVKVYKSGGDKFLWFLRKGGISREPDYIAEINDKKIEFEFQYAEKADLEF
                     YDFKVSKIAKSKKGNREPIKDKFIIYIYKPSYQYAIFKAEWVFNNSQYGMVEAWRTYA
                     YRVPKDKFLSILKPDTNLKSICKRIDAKNFILNFQHQLLDINKDKLSNLLQGVIDEDK
                     IVKLMPKDLDSFFKVCFILDHLNKIPQNANLWLIYLLTYINKDILLEDITKIVYCIDF
                     LYSKIDVKPNELNQLINKVEELILKIKSFYQDDGSYKSSLTSSPLEETRYALFSINLL
                     EDLIQDIIYYYSVSELQPIKKIYENVNDIEKTSKLIREAE"
     gene            complement(60260..61312)
                     /locus_tag="PLG69_00250"
     CDS             complement(60260..61312)
                     /locus_tag="PLG69_00250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPI41967.1"
                     /translation="MEPTDYKNRTYLSAIEASKFLGITVEMLHNIVNQQLIKAQIAAS
                     GQMRFDLKELKDFSANLKYKSHSNNIDIDKVNTLEINGTIQKIYIKNSMKMDDLEDNS
                     IHLMITSPPYFDTKMYSGEPLPGDLGNIHNIDEWFEKIGEVWKEVYRVLQPGRKAFIN
                     IMNLPVRLEKGKFRTLNLAGRTIDLCEKIGFIFKRDIIWQKTNAVRAHFGTYPYPGGI
                     LINNMHEFILEFDKPEKKSFNKYGHLTKEQKEQSKLDKDFWLSIKKSDVWVMKPQGSG
                     DNRNHIAPFPYELPFRLIKAFSYVGETILDPFVGSGVTLSAAADLKRNGIGYEIYPEI
                     AYEAVKALRYHQTEIW"
     gene            complement(61707..62387)
                     /locus_tag="PLG69_00255"
     CDS             complement(61707..62387)
                     /locus_tag="PLG69_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41968.1"
                     /translation="MPRIQSTGKCHFCFGTFSKSAMTKHLASCKAMQPAWESSLEYDE
                     TQSVKCFHLLVEGRYLPEYWMHLLVTANISLKRLDTFLRDIWLECCGHLSAFKIEDKT
                     YSVMPMPELKEKSMRFKLNEVFYPGIKFYHEYDFGTTTFLTLKVISEMELKAKGRFII
                     IMARNDPPEKKCYVCGKPATQVCVACIWDSKGWLCDKCASKHKCGVDMLLPVVNSPRV
                     GVCAYTGE"
     gene            complement(63198..64007)
                     /locus_tag="PLG69_00260"
     CDS             complement(63198..64007)
                     /locus_tag="PLG69_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPI41969.1"
                     /translation="MPDIKLFNGDALEIIKQLQSKSIDLIFADPPYNLSGENHLTCKS
                     GKIAKCDKGSWDHIDDIDEFNKKWIEECIRVLKDNGTIWISGTLHNHPSIGVILKQLN
                     LWIINDIIWFKPNAAPLIQKNRFVPSTELIWLAAKSKQYYFNYEMAVRLANGKQMRNL
                     WELSAEKHKTMHPTEKPEKLLERIVLIGSKQEDIILDPFMGSGTTGAVAKRLNRSFVG
                     IEIDQTYYNIAQKRIENTNVETNLVQFLEKLTHKTSSQLDFYSTIEKSKNY"
     gene            complement(64000..64800)
                     /locus_tag="PLG69_00265"
     CDS             complement(64000..64800)
                     /locus_tag="PLG69_00265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPI41970.1"
                     /translation="MQINHIYCGDCLKVMVTEISASSIDLVYADPPYNLSGKSLTLQN
                     NQTGGPFYKMNEDWDTWNYEDYLEFSENWISKVRIILKNTGSLYISCTHHNIAEIILT
                     AKKTGFKLNNILTWYKTNAMPNITKRTFTHSTEFVCWFVKGKKWKFNYEVIKNFNPHK
                     TKDGKSKQLRDFIDFIEMPIVQGKERIKGENGRALHPTQKPEKLIELILLASSDENDL
                     ILDPFFGTGTTGFVASRLKRNWIGIEINEDYCNIAHNRILGRKIKENA"
     gene            complement(64791..66590)
                     /locus_tag="PLG69_00270"
     CDS             complement(64791..66590)
                     /locus_tag="PLG69_00270"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HPI41971.1"
                     /translation="MRKPWSISTTVRNPERLRDFLRVLKQLEGQSFDTSNQIKYQVLL
                     IKERIYKPLNIPVDYLKYYEEPETEIPYKVAEQIFIMQKYEDPPMRGRQSVNPLNKLG
                     FSIAREGYGPIIITDLGNKFLSDDYEIGYIFFKSLLKLQFPNPWSADFSEKEGFDIMP
                     LITVMKLIKKINGEAEKKGLDKNEFNLFVPTLIKFDQVDEYVQRILEFRKAKNKDEYI
                     NDFVKMFYQTTTIPANKINNLFDYGDNIMRYFRLTRYFKVSMDPLGSNWIIDLEPSRK
                     VEIEQLLDMYKGASIKFETVTDYLNYLSDIEQPLLPWEKRDNLEKVVQTLKYDTNNLI
                     KEGKLQISQEQQTFLDMEIHDLDKNQLEKYKDQIRAFNLELKERIKKVKLMSDLNKVE
                     EIIVTLQDIKKLREYEPEQFEKLVTEALKIINDEIQIKPNYPVDDDGEPVSHSPANKP
                     DIECYYKKFKAIIEVTLNSSKLQWVLEGQPVMRHLRNFEIQHQNDDVFCIFIAPRIHI
                     DTYSQFWISVKYEYNGIPQKIVPMTSEQFAMLLDTLLLLLKKGKRFSHIELYELYTNI
                     VNESKRLVSFSNWALFIEKSLADWQQRIIKRCR"
     gene            complement(66685..68058)
                     /gene="mnmE"
                     /locus_tag="PLG69_00275"
     CDS             complement(66685..68058)
                     /gene="mnmE"
                     /locus_tag="PLG69_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPI41972.1"
                     /translation="MSKISEVICAPVTPLGFSAIAVIRLSGKGCIELVSNHFSQSQKL
                     LSSPSHNLIFGTFYAETGEPIDEVLISVFREPHSYTGEDVIEISCHGNPNLVNRILQT
                     LLLSCRLAKPGEFTLRAFLNGKMDLSQAEAVNDLIQAQANQAEKAALMQLKGFLSKYL
                     QELLARITELRIRFELAIDFADQDLPLPDSNALYQELLDIIKTAEELKSTGEQGRRLR
                     EGIKICLTGAPNVGKSSLFNALLQQNRAIVTPHPGTTRDYLEEYLSLNGFPIVIYDTA
                     GLREFPDDIEKEGITKSYELMQESDLILYLVEATTITNPNLQFSDLLSPSSLPPDLHS
                     KTLVVFSKADLLQENTPQISRGIYCSVITENGLKDLTDAISKRLMLNTELPNKPIIIN
                     NRHLVALSKCLQSLQQAKQSIEENQGYEFIAFELISASNALEEILGVITTDDLLDKIF
                     SEFCIGK"
     gene            complement(68051..69919)
                     /gene="dxs"
                     /locus_tag="PLG69_00280"
     CDS             complement(68051..69919)
                     /gene="dxs"
                     /locus_tag="PLG69_00280"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPI41973.1"
                     /translation="MILEQITEPKQIQALTVSELKILAKEVRSRIVEVVSKTGGHLAP
                     SLGTVDLTIALLHLFDPLKDRIVWDVGHQAYSWKILTGRNAQFDTLRQYKGLSGFTNR
                     EESPYDAFTTGHSSTSISAALGIACARDLNNEQGHCIAVIGDGALTGGMSFEALNHGG
                     HLQKDKFIVILNDNEMSISKNVGGLQKYMARMLASKSYNVLKKQVWDFSYTLPANIRR
                     SFIYGAQKLEESMMNILVPNIIFEDLGYKYVGPIDGHDIEHLLKIIKRVKNFMVGPVL
                     IHIVTQKGKGYPPAEKNSELFHGTGPFDLKTGKQISNGKQTWSELMGSTLITLAQKDP
                     KIIAITAAMTAGTGLTKFEETYPDRFFDVGIAEQHSVTLAAGMSTKGLKPFVAIYSTF
                     LQRALDQIIHDVALPKLPVVFCIDRAGLVGEDGATHHGAFDLSYLNFVPNMIILTPST
                     AEELSAMLFWAANYQEGPVAIRYPRGTAIHSSVHKSFTNFNPFPAIIHSNKGKIALVA
                     CGDAFFTAEAVYNLLAKDKIPAQLIQLLSVKPLDENTLLSLASTCNYIFTFENNAVIG
                     GMGARIAQLLATEPVKVINFGYPDHFIAQGKNSELLDEIGFTPNKLHKQIVQTLNE"
     gene            complement(69939..71855)
                     /locus_tag="PLG69_00285"
     CDS             complement(69939..71855)
                     /locus_tag="PLG69_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPI41974.1"
                     /translation="MFQLSFLNASLLFFALATILPLLIWLLAKKKPQKIIFPTLRFIK
                     LSKEQEKSRSKLKNILLLIIRMLIILLVVLAVSRPMLSSPKLKPSGKHPPTAIAVLID
                     TSYSMDYAERGKSSLQYAKDALKKINSKANPDDRLIPVSSDENWNLLHSQIYAGSLPE
                     TLIDQLKITFHPLSLEEMLALAETKLAESQMPNREIYLISDLRIPPAELKTNIPIALI
                     PLPETSEYENLAVISANALPQLVEKHNQQLIQFTVANYGSTKRQDVLLKAVVNDIKLA
                     EKFISIPSQSTITETIPVELRSDGWQSGYIEVNDERQIMDNRCYFAFPFYSKPRVAVI
                     SQSSNLPPILATVLRVYSSSTPQVISPDALSLSDLDKFQLFIAYNCGPLTSRLLEIFT
                     TLQNRKSGILFCLGNNLSADLKSYLNNTFGLEIKDRTQKSITIDNINAHHYISSLVSG
                     KPLKNPILTDYWQAVNKSAGVLISAQNFPLAVINGKQSLWLWNISADNNPFFIDPAFP
                     VLAFRTLDYIAGSEIPETNAKIGQVLTFNRLKLPTGEIITSRRYLATEPGIYIFEPDT
                     PRSYAMAINIDYSDSEARTNFPKGVKNLGEKWEDKLFFSRLGHDLWKILLAIAFALMI
                     VEIIIVKTEEARAK"
     gene            complement(71856..72923)
                     /locus_tag="PLG69_00290"
     CDS             complement(71856..72923)
                     /locus_tag="PLG69_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41975.1"
                     /translation="MSKIKFLMPKSLIYIILLISPAWLLLAQQVTPLPITSYDYALPA
                     NNVSPIAMGTGALNLTNPEDPFCAYGNPALMADNKLGSLSLSFLLSTQEDLEFYEALS
                     LSNTLKDKQFKYFVLNTGPVSFSYQPVSAVHISQFTNNGMNSEYYDYKMDKVQISWGG
                     RDENYPKFAAGINLKYLSGRLVYLKEHKEGYNLIRDGFIDDKAKGISGDLGFTYKMGN
                     IVYSASFYDLLSMLWWENYDSVTLKRRAALGLGSEGTNSHYFAGIQGKIAQEPETTFH
                     FGYGYTFNLGKSNYSTAQNRNSLDLRVGLYSHDFYGTDNINFTLGGGYYYKNFRFDFS
                     LNNIGMKLSDSDYLFSLSAGI"
     gene            complement(72923..73858)
                     /locus_tag="PLG69_00295"
     CDS             complement(72923..73858)
                     /locus_tag="PLG69_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPI41976.1"
                     /translation="MTDTSITANRKTSFFLSAIDLITLIFCGWILLYMCFGITRSPEV
                     IKHIPVYLAIFVGVLFLAWLQKQPGWSYDPQNPSKRYQILSFFRGLYPVLLFGYFYTS
                     GHAFNRIIFRDWLDPFFMGIDQFIFGYLPSLVWGKLYSYWAIQELFHFAYFCYYPMIA
                     GIPIYLYFTQKDAFREVIFNLTFVFYCCYTIYSVLPVIGGRFLPEAMALTKTYRGGPF
                     THIMVFIYRTSNHLGGAFPSSHIAIAIVLTISALKYIRPLGYICTVITFFLSLATVYC
                     HYHWFIDAVFGILTGIAGYYLANWTYYYLREKGFN"
     gene            complement(73869..74996)
                     /locus_tag="PLG69_00300"
     CDS             complement(73869..74996)
                     /locus_tag="PLG69_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPI41977.1"
                     /translation="MINVIPVDNKKKLKDFILLPFKLYKNEPNWVPPLIGDQKKFFNP
                     KKNPYYYHSEVKLFLALKDGEPVGRISAHSNTQHNKEHNENIGFFGFFECIDNQEVAN
                     ALFDAAFEWNRYRNFTSLRGPMNFSVNQDCGLLINGFDSPPMIMMPYNFPYYPKLYEN
                     YGLTKTMDLYAFISEYHSIPERVAKMAEIIAKRTNVQIHSLSYDKKQRKKDIETVFEI
                     YTRAWQYNWGNVPMTKAEFDHIVAELLPIADPDMVFIAEVDGQLAGFSLTLPNYNEVL
                     KVMQGKVNPITIIKALQAKKHITSVRVITMGIIKEFQGKGIDTLLYYHTFKNGLPKGY
                     YRSEFSWILENNIPMINAAEKLGAKIYKTYRLYDKAITNRK"
     gene            complement(75018..75662)
                     /locus_tag="PLG69_00305"
     CDS             complement(75018..75662)
                     /locus_tag="PLG69_00305"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPI41978.1"
                     /translation="MIYLFVILIIIIAYFYGCFSTARIVAKSFRSLNVYKIGTGLADT
                     ENIYTNISKPLGTLVGAIDVAKASLFLLVVEFLLGFIDIKVINYPDFTILYSENLMLI
                     YGLSMLIGHCLPFTHRFRGGRGIFTYMGILAYIAFLPTLISAVIAWLIVLHFKQIRFA
                     QYLIVILPVILYIIFYTLFHYHKSSPPYFITLLLGNAIMMGILNIIVSKKLGEF"
     gene            complement(75659..76360)
                     /locus_tag="PLG69_00310"
     CDS             complement(75659..76360)
                     /locus_tag="PLG69_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPI41979.1"
                     /translation="MNIAENIKHLQDNIADILSRLNRKDEITLVAVTKTHPVEIIETA
                     LQSGIEHIGENRVQEARRKIPFLKVPYKGFHFIGHLQSNKINQVLELKPFLIQSVHSL
                     ELAKKLHLALGRTNRTQDILIQINSSQEFSKNGFSFVEVKNAICQISTYSNLHIKGLM
                     TIGMLAEPEVSRPLFARMKSLFEEITALAIPNVDMQYLSMGMSNDYVQALEEGSNMLR
                     IGTAIFGERSYGVNE"
     gene            complement(76357..79977)
                     /locus_tag="PLG69_00315"
     CDS             complement(76357..79977)
                     /locus_tag="PLG69_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI41980.1"
                     /translation="MKKVLVLGSTILICLSLAAFNTEINIDPFAWQNKKPAEELPVTI
                     NPGEPILPFTPVNILLPFGHRYLNSTVQLSTAELVAENITFAFAGPQLPISDQVFTNS
                     FPPANTIPENRLYPSQQWQFLGTQYFRGYQIALFNVYPYRYNPVTQKLYVSSQISISI
                     NSEFSEDEACYQANFYTPGTKTLQIINSLICNPDKIPSYQLANNYRNVPVPNRIIDLS
                     TPKQMIIITNNARIGWFSEYSAWRSAKGVSNSIYSMEDIVSAYPGVDNAEKLRNFIIN
                     AYQTWANSSQPLEYVILGGDDEIVPERGAYGQVGDTVDARMPTDIYFSNLDGNWNANQ
                     NQIWGETNDNTDLIPEVHIGRFPAETQTEFNNMFRKIQYYVDNNTYSNNYAVFMGENL
                     NNNPLTWGGDYKDDVAQYLPAEYYLQTLYQRDGTYSADNVYNAISNGAGIMNHMGHSN
                     EVSLIGQSNTTVESLTNTEYGFLYSQGCYPAAFDQRTSGNNEAIGEHFVTAAGALFSF
                     IGNTRYGWYSPGNINGASQFYDRQFFRGMFQQNCPVLGDALTFSRLQNLNSALSNDVM
                     RWCYYEVVLFGDPSIAIKPYDPNLPLLNLESYTFSDEEGDNDGIINPGEIIRFYPLIS
                     NNINWADATNVSLHIENLPPGVELLSDDIIIPQILAGNQSPANIYFRLQLSDDMSFGI
                     YSFNLVLNSQHPITFQSTGAHRFPIFFQITLIDNRFPWETIVNGKSAPLVADLNSSPG
                     LEIIYADVYGNSYIIGSNGELLNTLEAPSGMLINRSFASGPIDSDDQNDLVFCTRSGN
                     IFALRLNGDIIFNYQTDTTFLFTPMLADIDGNGILETIVGGINGKLYAVSSSGQLMNG
                     FPFELGGIINSELAAADFDGNGSFEIVAGSTNGLLTVVGANGIVRNGFPVQLNGPVTG
                     FPTITNTNRIVCSTPTDIYIISPEGNIIATRNINTHITGGFAVGNITADNTGTDIAGV
                     TLNGILYAFTDSGIDLPGFPVNIGENFACPPLLVNLDSNPQLEIIVHNYMNSVYVYKA
                     DGSPLSGFPFINIYNGSTPATLVDFDNNNQTKLVLGFSNGVLMLNLRRNTAHLGPWIT
                     YRGSALRQGSFASTGFIDSSDEYNIPALNALLGNYPNPFNPETTICYTTKANVQAKLE
                     IFNIKGQKIRTLIDAIHSEGNHSIVWNGYDDFSRKVASGVYFYRLNIEGKTFQRKMLL
                     LK"
     gene            80222..80983
                     /locus_tag="PLG69_00320"
     CDS             80222..80983
                     /locus_tag="PLG69_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPI41981.1"
                     /translation="MQIPFNMNRLKDTEIHYPQRFSNLLTKNYGLLFYNEGNKASQES
                     NHAVILDLIGVESSLRDIEFFYKSKGIHPCIYPALTNKELEILTPLLDEHNFTLKIKQ
                     SDFFLHDHESSLRESEELKIERIHQLDIDIMETIAIEYGGDWTIRVVERHLTHPSYHF
                     LGGYYQGELATMASVSIFAGYSRVDDVFTREKFRGKGFAGGMIHHLINYHRNISQNYL
                     YLQSDLPSAIKIYEKAGFSRLPKDFQFWVAYKEHN"
     gene            complement(81146..81754)
                     /locus_tag="PLG69_00325"
     CDS             complement(81146..81754)
                     /locus_tag="PLG69_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41982.1"
                     /translation="MKKGLLILLALIIASSVFAFTKGTINPGGTISFASHKPNSDEKA
                     ITMFSFTPQVGYFVMDNLSADVLINYTYEGQSKGDSNEENSLSALGIGIGGRYFYPMA
                     PGKIYGGLDFLYNTNSWKVSDYDGGCNAMYLGLKAGYVLPIVENVYVDFGLRYQMGLG
                     DYGGDDYEHALDEYKENEESTFGFNLGLQIFFSNDFFISLLE"
     gene            complement(82074..83351)
                     /locus_tag="PLG69_00330"
     CDS             complement(82074..83351)
                     /locus_tag="PLG69_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPI41983.1"
                     /translation="MKLLKSVSLPTSSTALKTVNVLFEDKIIKLSTDEIEVEEDVQII
                     DAKGLLMLPGGIDPHTHILAKNADAATDLAKITKIALEGGWTTLAELSYHTQSPVFNN
                     SALIKKIALVNANSFTSLALWGHIDIGDYPYHAESAQEIWSKGIVGIALAVPSPNPAI
                     PDITFTEIMDLFFDIYESDTSFAFQGYDYESYQEFSPLAQLDAIKKILRRMQENPIHI
                     PRISSYPTIEFINSVSKRSDISFATCISDLMALYNPDVFSTPFLCDYADFADLLFDLL
                     RSNKIYLLSNCVAPPVKNPNYCELFQGCNPELMNYSYLWVLSELWKKRKVPLATCLKM
                     TSENAAKRLGIYPEKGSLDPGSDADFVLYDPEATTIVKGNKGKDIELSGSFKAIYLKG
                     NQVAGKDFATIQQGSFLARSTNPKRRHNNSSWI"
     gene            complement(83441..84295)
                     /locus_tag="PLG69_00335"
     CDS             complement(83441..84295)
                     /locus_tag="PLG69_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41984.1"
                     /translation="MCRKWLFLILLILTACSTVKIPVEPQAKIDADNHLRFALQMENN
                     RDFSSARESYIFLTENYRSFADIKGMLACLSGLARIELENNNEKAYLQYKNQMAEMIQ
                     NTAPELAYYLLLLELYNYQLENNYSAISQKAISKIDYPAEVNLKLAIDKLQADSYLAI
                     AEEKQVKELINLSKKMEKQQKKAKYNDPELLSSAWYALAYYYYRTADYETANFYADKA
                     QDWDYRFGNFRGMAHCLWLKAQIAAAENNIPTAKRYWKKAEGIFSSLRDNVSLEELNT
                     TMQKLLKE"
     gene            complement(84285..85367)
                     /locus_tag="PLG69_00340"
     CDS             complement(84285..85367)
                     /locus_tag="PLG69_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPI41985.1"
                     /translation="MIDKIKHSRKIVFAFISIPVLLLLLAMVFIAIRQNLLEKKYVYY
                     STLANAMGISTQTPVLYKGFEVGRVRDFSLMEDGNIGLEFYILNRYKNIMVTGSILAR
                     NTNPIIGKTTLEFIQNPNSREHLQDKSTILSSDFPEGKKQLKLIAPQTSDPISLILDN
                     LAQLSSSLNEDYNADKGSIPRFLVNLADASEKANASMNKVEEITTELSILTANLNQDN
                     NPESGVLLRTLNNLAELTQGLNNRMNELEFILASAQTAIDNYKKPDSLLIKLLDPEQD
                     KILQPLSETLNNLESATAELVKILATVNNPELRLLLSNLNDSLAKARKTLEGLNNNPL
                     LRKGISPSAEQNMPSPKYIHQVPNVP"
     assembly_gap    85574..85673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            85968..87107
                     /locus_tag="PLG69_00345"
     CDS             85968..87107
                     /locus_tag="PLG69_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPI41986.1"
                     /translation="MKFKDSSQLTIPIMEEMPKFNSVVILNQEKLIEVLEEIEKIDNV
                     NLMKNRILALKAIINNYNNDREGLLNIDSNGETISLRRDILFSELNQILESQTLERAQ
                     YYLKRLKNGIKEEKTSKINDINLHRWKEYDDILTDSLWLFDKRNTSGAHLGWYWGNFI
                     PQIPHQMMLRYTKKDDWVLDTFLGSGTTLIESKRLGRNGIGIELNAEVANKAEKLIQM
                     EANKYDVISNVIIGDSRTIDLKPTLQKYNINKFQLLIMHPPYHDIIRFSKDDKNDLSN
                     TETTEEFLEMFKAVVNNVTSYLEKGRYLVLVIGDKYSKGEWIPLGFYCMQEVLNSGYL
                     LKSIIVKNFEETRAKRNQQELWRYRALVGGFYVFKHEYIMLFKKK"
     gene            87121..>87411
                     /locus_tag="PLG69_00350"
     CDS             87121..>87411
                     /locus_tag="PLG69_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41987.1"
                     /translation="MPLYSKKEILNLKLNSIKSKELRVLAENLGISTKGSAAEIIKRI
                     LEMKPQPPENIVDVYIKSIFRQSIQERKELISDDDLKNELSKVKSFSWGTKQG"
     assembly_gap    87412..87511
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<87512..89633)
                     /locus_tag="PLG69_00355"
     CDS             complement(<87512..89633)
                     /locus_tag="PLG69_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPI41988.1"
                     /translation="MKIAMKIILCTMLFLPALISATLPTCYYTYAQIYALLQSHQQSH
                     PDIAKIYIIGYSQQDHIPIYAIRISDNVEIDEYEPALLFVGQVHAEEVLGVQTTMANI
                     AEILTYRYQQPYAQWISQLDLWFIPTLNPEGHNVVTENLDVSYRKNKRDNNNNGIFDF
                     DPVTGGDIDGVDINRNFAFNWVHGDTLYCPVGDETYDYYRGPAPMSESELIGFQQLCE
                     QRKFIYAVCWHSSRTGNFSEKVYYPFNWKYIRPSPDLNFAASIATGFASQIIKQSGTG
                     TYEAYPNLSRQGAFHDWLYQQYGTIALLVECGTSDIQPDSTVMVGTVQRCSNGIRWLI
                     NRALMFSSAVPSSSMLTGTVTAAGIPTEAEIIIQQHNAPWFRPRTSFPQNGKFYRPLA
                     SGSYTVIARKKGYYDKIINNQIVNNGSWTNIQINLEPKPPATLRIGVRCGSEPVSARV
                     IIGDIFPDTLFVNGFANLNTYAGDYPIQIYALGYTPYIGTVNLTEGHNHLEFNLSPAT
                     IVFSEDWENGSSGWEIEGPWKVQNQIAASGYAITDSIGGYGFYAINCNVWIKTINPIL
                     IPATNQTWLIFDSHLYTEWNYDPCRVEISPDGNNWQEIWLKSGQWDSFRKEFVSLSDF
                     AGQSIYLRFRLTDSSNDIDLTDPGWTLDNIMIVCGTATENSDLNNDLTPLTLLYKNYP
                     NPFNPETTLSFTLAKNSRATLKIYN"
     assembly_gap    89861..89960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            90209..90418
                     /gene="rpmE"
                     /locus_tag="PLG69_00360"
     CDS             90209..90418
                     /gene="rpmE"
                     /locus_tag="PLG69_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPI41989.1"
                     /translation="MKKGIHPKYELVTVTCACGNTFQTRSTKDKLQVEICNVCHPFYT
                     GKQKIVDTAGRVEKFNKKYNLKQEN"
     gene            90498..91472
                     /locus_tag="PLG69_00365"
     CDS             90498..91472
                     /locus_tag="PLG69_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPI41990.1"
                     /translation="MQEKKEINVGGQAVIEGVMMRGPDKLATAIRRKDGSIELLKTPF
                     ISITQKKKFLGLPIIRGFVSLIEMMIIGIKTLTFSANRFELDLLVEEESKGKKTKKRS
                     KTAENTTNIVSYIIAFGLAFLLFGWLPYKLADWMHLSRKDILFNLFAGAIRIVFFVLY
                     VYLISLMKDIKRLFRYHGAEHKNVNAYEHNCPLIIEKIQDWSTIHPRCGTSFIFFVLL
                     ISILIFSLVDTLVSAYILHTPVPVYLRLAYHILLLPLVSGVSYEVLKYSGKNLNHPIV
                     KLLTVPGMALQHITTQPPDNDMIEIGLVAMKAALDMDLSEHKVKIIEE"
     gene            91478..92545
                     /gene="prfA"
                     /locus_tag="PLG69_00370"
     CDS             91478..92545
                     /gene="prfA"
                     /locus_tag="PLG69_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPI41991.1"
                     /translation="MLPQEKLQILENELKEMQEKISDPAVINDPQKYRNCLRRFKELR
                     EINAAWKHYQTLKKHIEEVNALEKTENDPEMLDLIKDEERSLQQQLEQTELKLKDLLI
                     PADPNDAKNAIVEIRAGTGGEEAALFAADLFRMYSYYAEMKGWKLEVISMNQTELGGY
                     KEIIFQLSGKDVYSYMRFESGVHRVQRIPVTESSGRIHTSAVTVAVLPEAEDIDIDIQ
                     ESDLRIDVYRSTGHGGQSVNTTDSAVRITHLPTGIVVTCQDEKSQIKNKAKALKVLRS
                     RLLDEEISRQEQEIAKARKAQVSTGDRSAKIRTYNFPQSRVTDHRINLTSYNLDSFLA
                     GNIEEFIQALRLAWRNEKVNT"
     gene            92546..93811
                     /locus_tag="PLG69_00375"
     CDS             92546..93811
                     /locus_tag="PLG69_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPI41992.1"
                     /translation="MSVVQIVLILLVLLFFLLLSFLFAGLETGMLSIDQIKLEQEAKK
                     SKSKAAILQFVRQPDRFLGTILIGNNISNVIMASLSTYLVNRLFAPVFDARYTSLVVG
                     IVVLIFGEIAPKAIFRDNAETLVPLLFPFLQFFYYLLKPFVMVVTWLNNFLRKLLKLE
                     EGYQYDYLTKDDLSFLLSQAANAETEDNQMEMIEDALDFTELDAGNVMVPRTEIVAIQ
                     ENTPLSEVINIVRKEGFTRYPVYRNNIDDIIGILIIFDILKKDCTPDMIAGDIIHEPL
                     FVPENMDLDVLLKEMQTKQRSMAIVVDSFGGTAGIVTMEDILEEIVGDIEDEYDIDED
                     EHSVQQVGPNTWLVAADTEIDRLADDYGIELPEGDYETIAGLILDRLERIPHQGQVIN
                     IPPYKIQILQATAKQIIKVKIHKTSKIEV"
     gene            93813..95489
                     /gene="ftcD"
                     /locus_tag="PLG69_00380"
     CDS             93813..95489
                     /gene="ftcD"
                     /locus_tag="PLG69_00380"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPI41993.1"
                     /translation="MKLMECVPNFSEGRDKTILDAIATAIKNVKGVALLDIDPGADTN
                     RTVFTMAGEPEAVVEAAFQAIKKAAELIDMSKHKGAHPRMGATDVCPFIPISEMTMDE
                     CVEYAQQLGKRVGEELGIPVYLYEYAATKEEWRNLSNIRNGEYEALPEKAKDPYWKPD
                     FGPHTFNAKSGATAIGAREFLIAYNINLNTRDKKKASEIAQIIRESGRPARDENGKLL
                     KDENGNPIYIPGLFSHCKAVGWFIESYDRAQISINLTNYKITPPHLVLEKVRELASEM
                     GMQVTGSELVGLIPKSALVEAGKFYLQRLQESTGIPEKMIMQTAIQSMGLAELAPFDL
                     NKKVIEYAIAGKDLLVNLKVDAFCDLLSTDAPAPGGGSVSALCSAISGALSAMVANLT
                     IGKKGYEKVTEEAMKLAPRGQEIKQKSLEVIDKDTDAFFALMDAMRLPKKTEEEIAYR
                     KAQIENCTQQAINAPLETMRLAFSALELASKIAKIGNTNALSDAGVAALTAFCASQSA
                     YYNILINIPGITDEEFKQKTLAEAKELLEKCKIIAYSVEKYVCEQLKGKG"
     gene            complement(95701..96375)
                     /locus_tag="PLG69_00385"
     CDS             complement(95701..96375)
                     /locus_tag="PLG69_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HPI41994.1"
                     /translation="METMQNHPYKPFIPKDAKVLLLGSAPPYRFCTGNANDLKNKDMD
                     YYYGSNSNLLWEILFTAFEPDKIDSISYLRSLEEDRKIKTAYQVDFLQSFLARNQLGM
                     ADILLKFSRKGTSAEDYKLTVLEYQDILGILYNNSSLTKILCTSKNRVHYWLMDYLKQ
                     QKHYDLIEIRDNGNSLILKNNPHREIKIGILPSPSARSVMRYANKTEFLQQVSQIYKQ
                     AITNDE"
     gene            complement(96552..97457)
                     /locus_tag="PLG69_00390"
     CDS             complement(96552..97457)
                     /locus_tag="PLG69_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41995.1"
                     /translation="MKRHFLLFILLCLSRFLFSSYPLQLKTNNLAIYAQNPVSQPLAE
                     MLKAMDENIDSFQMELGVYVDALAPVYLIENNRSYQTLALGKAKIVEFSDAFYSGTEK
                     RIYVKPLASIQENHRKILMHEYIHWYLEQVFTQTPLWFHEGMATHFSRQMGFEQYLYF
                     LQQSFLGEKSDLFRLSYSYPEKKEDWSLFYLSSTMAISYLKNKKNEQWNSFWEMVAQQ
                     HRKNLQAPFTDCFNRAFHTTFYDFHKEYARYIKHLRYQYLFWSINALLALLIPVILII
                     AWRIRKKRLASLPDLPLPEDEETEM"
     gene            complement(97548..97808)
                     /locus_tag="PLG69_00395"
     CDS             complement(97548..97808)
                     /locus_tag="PLG69_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPI41996.1"
                     /translation="MRIDVLLNKLCLTKTRSIAKNACDKNLVLINGKPAKASADVKAG
                     DIIIFRLYNAEHEIRITQIPEGNVAKKDATRYYELLRQEDLS"
     gene            complement(97882..99081)
                     /locus_tag="PLG69_00400"
     CDS             complement(97882..99081)
                     /locus_tag="PLG69_00400"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPI41997.1"
                     /translation="MRILVLNCGSSSIKYQLIDMETESLMAEGIVEKIGEDIALFTYK
                     SEKYTKKKREMVVDNHEQGLQLILEALIDPANGVLNGLDEIDAVGHRLVHAGEHYSDA
                     VKVTEHVIQVMQECVSLAPLHNPANLKGIEAVKVNMPNCPQCGVFDTAFHQTMPAMAY
                     LYPLPLELYTNYKIRRYGFHGTSHKYVSLKAAEFLQRPLESLKIITCHLGNGASMTAI
                     KNGKSIDTSMGFTPLEGLMMGTRCGDIDPAIPIHLQQQLGLSVDEVNNILNKKSGMLG
                     LSHISNDMREIENEVLVKNNPKAIMALDVYCYRIKKYIGSYFAVLNGLDVLVFTGGVG
                     ENMPLLREQVCRDLEAIGIKLNLEENSQRTDGIQVLSANDSSVTVLKIPTNEELMIAR
                     ETKRLLS"
     gene            complement(99440..100012)
                     /locus_tag="PLG69_00405"
     CDS             complement(99440..100012)
                     /locus_tag="PLG69_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI41998.1"
                     /translation="MKYAFLLLLLAEIMMLSALTFEAEELNFYLENNLWEMDGLFHFA
                     NYDSLSAKELIFFPVPSDSFCLPPKIKALSVQDDKEGAVTLLNQDKNGFTFLLSLPAL
                     SFCSLHLDYTQTLKSNYAKYIITTTNSWGRPIPYAKYTLHPGISVQISSLPFPLQKQE
                     GRNYIWEFFDFAPEKEFEVTFQTIPAEDQN"
     gene            complement(100009..101016)
                     /locus_tag="PLG69_00410"
     CDS             complement(100009..101016)
                     /locus_tag="PLG69_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI41999.1"
                     /translation="MKRIVLALILFIATISAWSNPIVPNLISRFWFEQENVLEIELSA
                     FVGTFQTAEIIYLDAEDSLTIQVNPNAVTVQQINVPQSVNTPASGFFTIRIPQLYEET
                     VHWGTGLENDLSPLNGTQCAVQILYSNLIGDNVYGWAKDVSPAVCGPNYCSAKFTYRL
                     ECTDLSGLPVAGVSVYNSQSAGGIFINPAITNSAGIATGELYPMKTQIWVNNPITNEI
                     AFNTQFFAEPGALIPFSVQLPVSVEENIVSIPAGKLNLYPSVLNPHWNESIRISYDTK
                     LTGKAELVLYDLKGRHLAEKVYDQNEIEWQLPKLSSGIYFVRLTNQGKALGTAKLIIL
                     K"
     gene            complement(101694..>101971)
                     /locus_tag="PLG69_00415"
     CDS             complement(101694..>101971)
                     /locus_tag="PLG69_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPI42000.1"
                     /translation="ICLHDKIAAFSIFSPQTPDMVTEHFEKFDPEKKGAAQLINWETA
                     KYLQNRYKYLNREQDLGLEGLRQAKLSYQPEFLVKFLVTNLKSNAKK"
BASE COUNT        32331 a        18548 c        19252 g        31440 t
ORIGIN      
        1 cacaatcatt ctctctgaat ttggttctgt ggcaaagatt atttatatag atcggagttg
       61 cgtatgtcat ttagaaccct ttcccagagt gtatcaatgg gtgaaaaata ttcttccggg
      121 gtgatcagta catcaacatc tgggtataaa ctgtcagaag gatctctgct tgtatcaggc
      181 cgttgaaagt atttaacaga tacactgcac attatgcccg tattaggcaa tctgaagttt
      241 agtccctctc cataagaaga tggttttcct ccggtaggac ttccgtacac tatagcaagt
      301 ccgttatcct gacaaagagt agcaaacata actgccgagc tgtatgtttg acttccagta
      361 agcaggatca attttccatt atactttttt attgaatcat tactcatagc attgggaact
      421 atttcactaa gagtccgagg tctaaggaat ggagatttac catcataaaa aatcacggta
      481 aagggcaatt cagtttccaa tacttcataa cccaagtcag aggataatgc cttattcatt
      541 gaagtcagat tcatttgata gtgattgagc accaaatcac ttatcttcat cttcccaccg
      601 aagtagtaaa tagaatcagg cagtgagcaa aaagaaagga gtatatcacc catggtagaa
      661 ttgccaccgc tattgtttct taaatcaata accagattat ctatccctaa gctgtctatt
      721 acagcaaagc tctctttaag aaatgagatc caccctgaaa atggagtatc ggatgtccct
      781 actggatctg acattgcatt aaattggatg tagtatacat tgtcttctgg aagggcttgt
      841 ccccaatagt tatcatcatg taaagccgta ataccggttt tctggttgcc ccaaagagaa
      901 ggtctttcaa cttcgtataa attgacttct gaaaaggttc cattctgttt aatgatcaac
      961 tgtaaaacac cgtcgggtct atccagattc aaggcatgta agttcgctga gtttctgagg
     1021 aaaaaagaca cttggtctcg gaaccagtag acattctccc ccgtagttag ttgcattaat
     1081 tttgtcataa aggtctcagc atctatccca ttaatactta ttactttact tccaattaaa
     1141 tctttgaact cggtagaaat catttcaaca taccatccag attcatgcca ccacaatcct
     1201 atagggtaaa ttggtgatcc ctgattggaa aatgatgata ctcttgtatg tccgtcatgt
     1261 aaatcatggg taaaagctct caatctggct tcaaattgca gagtatcagt tacagtggcg
     1321 agacattcca tgaatgcttc aactttctga ttgaaactag tattatcagt ttctatgcca
     1381 ggatgagaat caagtaatat tctataaaag taattgaaat cttcctgaaa tacattcagc
     1441 tgagaattgg gttcacgaac tatattattg gcattcaact cggagtgcaa gtccactgct
     1501 attatagtaa tgaaagacgc aaccattaag taacggtaaa gtagattaat aattctacca
     1561 atgcttgttg agttgttctc aagcttttta ctcattttta ctccagtatt gatttgaaca
     1621 aacaatcttc aattactgca aaagatttta aatatcattc acattatctg tcaagcacaa
     1681 actaacaatt gaccaatctc aactgcatta ccaccgtccc gatcagcgag atattgtttc
     1741 cctgttttcg ttttagctca caatttttag attgggaaat cagtaaaaac tcggttgtaa
     1801 gtgaaatagt tagtgggttg tggtttccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnng gaaatcagta aaaactcggt tgtaagtgaa atagttagtg ggttgtggtt
     1981 tccgtgaagg cagatgaaaa ttgtaagcca aaacaagaaa aaatcctttt aatcctgttt
     2041 atcccttact tatttcttct tttctctttt taaaatcccc aaaatctctt taatcctgtt
     2101 tatcccctaa ctatttcttc tttttattat ttttacttca gcagaagtat ctttcgggtt
     2161 tgcaccttat taccttgctg tgctttaaga aaatagattc cggagctaac tttcttgcca
     2221 ttattatcgt taccctccca ggaaagggta ttttttcctt ctggttttcc ctgaaaaagt
     2281 gttttgatta actgtccttt caggttaaaa acctgaacca gtaaaggagc gttgctgttt
     2341 ttacagagaa tatttatttg ggtagaaaca gaaaaaggat tgggttgatt aggtaaaata
     2401 gaaatttccg caacaggaat attaacttca tccccaactg caacaggaaa accaaaataa
     2461 ttctctataa tgtaatgaac aacttgacgg ctttggttga aatccataaa gtaaagagga
     2521 aagctgaagg ttactgtttt acccgaaaaa taagtgtttt gaacacctac tactcctccg
     2581 ttgagaattc cctgaggtga attattgtcg taagcggaag cgtaatgata aataggagtt
     2641 gcctgagaat ttacggttaa tgcttctact tgataaaggt gtccattaaa agaagcggga
     2701 actttattgg ggtcaacatt gatatccgga tagccggaat aaacactttc tgctttattg
     2761 aatcgggctt gaatagtgta atcagttccg ctgatgccta aaacttcatt gataaatgaa
     2821 tctgcagaaa aaacggtagg ataatcagca ttcagttcaa aagccaatcc cggatggaaa
     2881 acactgaaaa gaactttgcc tccataataa atatagttct ttaaaacatc tttaacttga
     2941 ttgggataaa tcatattaga aaaatcgtta ccgtgccaaa taatgacgga ataaataccg
     3001 atatcgcata aacgcaatgt ctctgtaaaa tcagccaaat caaaatgtgc agtgttccac
     3061 aagccgtcaa ttaacaaatc gtaaaagtta tctactgcct catccgttgg ctgaaaagga
     3121 ctgcttccgc taaaatctgc cgtttcatca ataattaaaa gtccgcagtt catactgaga
     3181 ggtctggttt taagcactgg agtaaaggga ctgctattat tatcattatc aaaggcacaa
     3241 agacgatagt aatagtaaaa ttctcctccc tgaacattag aatctgaata actgcataaa
     3301 gttggaggta aagtggcaat aagcgctccc gtttctgagc tatttagaga acgatatatt
     3361 ttataaccgg ctaaatcaag ctcggtattt gactgccagg aaagcttaac tatttctggt
     3421 aagggctgat ctataaagct aacaggtgtt tgaggtatta acctgggaat acactgtgtt
     3481 gccacagagt agctttcaaa accggaaaaa tctactgaag atacagcaaa gaaataaagt
     3541 tgatccgtat ttaagccggt aatactaatt tcattattgg ttgtagtttg agaataaata
     3601 aggttattct gcgaaggtcc ccaggaaata tgaaaatgag aaaaatcagg atcgggaaca
     3661 ggattccagg aaaggtgaat actatttccc gttccttcat cctgagccgt aagatttgca
     3721 ggcggagatg gcatattggc aaaactggca gttacggcag ttgaagcacg aattacggat
     3781 gcacaataaa caggatcaag attggtaaca gtatcatcat tgctgtgata gacagaagaa
     3841 aagttatatt caaagaaata aacaaccgga aaaccgtgct gccagaaaga atgactgtca
     3901 ctgctggatg aattaagata accataaaca ggttgcaaag tagtgtattg ggatgtaatc
     3961 attgcagcat aatccgtatg ggaaataaaa ccatcatagg gcattagcaa aaccctcggg
     4021 tcccagaaat tgggacttgt atttgctatc atatcgtgat ttatcatcac tcggatattc
     4081 atattggttt gatctgccat atttgcataa ttacttgaac cacaaagccc aacctcttct
     4141 gcggcaaaag ttacaaaacg aatagaacat ttgggctgat aattagttgc catcatcact
     4201 cttgccattt ctagggcagc aactgtgcca ctggcattat catctgctcc gggagcaaaa
     4261 atcattggat tatcgttagt gatggaatca tgatgtccac ctacaataat ataggtatcg
     4321 ggatatacag ttcccgtaat tgtagctaca acattatatt gcatagttcc ataccactga
     4381 aaactatgca aaacggaatt gttaatgcca aaacgctgaa attgggattt aatccagttg
     4441 gcaactgtca aatggttatt agctaaagca taacgcgtag aaaaattctg caagcactga
     4501 aggaaatata aaacgctatc ttccgaaaca gtattaatca aattttctat ttcattccgc
     4561 gtagaactaa ttgctgtatg ccaagaaaga gtttcagaaa aacgcattgg ttccagaagt
     4621 aaagggacaa aaggattttg aatcttctgc cgaagctgaa tttcatctct atccgttttt
     4681 attaacacta ccgaacccat ttccaataag acctctccta cggttgaaaa atcaaacttg
     4741 gagtatcttt caccaatcgt tattagatag aactttccgc tttttggtgt tcccagattt
     4801 atggctccgg ggtaattatt cctgtctgtt ccagcaataa cataagaatc gttgtaataa
     4861 tatatttcca aatcggtctc agcaaggtct tgaactgctc tgcgcagtgt tttatcttcc
     4921 ttatctaaag aaagttccga tacgggcaaa ccgattagat attggggaac agcacataaa
     4981 atacttaaag atagcagggc aaaaattaga aggaaaaatt tcttcatctg tatctccttt
     5041 tggatatcta tatacgataa aaaagcaaat aacattcctg ttaatggttt ttttacaccg
     5101 aggacaccga aaacaccgaa ataatttata attttttcat tcctgcctta tttgggagat
     5161 actgatataa gttacctaat gaaaattcag tcagataggt taaaactgtc tggatttctg
     5221 ttttcacagg aatggaaaat agcttattac tcaaaaacga tatatctatt ttgctcttac
     5281 atctttctct ttttctcttt gtaaaataaa tctcggtgtc tacaatgttt tcggtgtcta
     5341 atttttctct ctttgtgaaa ttcccacttt ctcattgaca aaattcttag ctgaaaacct
     5401 tatgcctgat agttcattat tggtgttata atgggctttt acagaatgga gaaaatatga
     5461 aaattctgat cacaggcgga gccggattta tcggttccca tttagcggaa aggctattgc
     5521 aggaaggaaa cgaagtttat gtgatagata acctttccac aggccgtctg gaaaacatag
     5581 aatcctttaa agataaaccc aattttcact taaatattgg cagtgtgctg aatcgtgaac
     5641 ttttggataa actgattagt aatgtagaac aggtgtatca tttagctgct gcggttgggg
     5701 ttaaatatat tattgaaaac cctcttttat cgcttaaaac aaatattatg gggacagata
     5761 atgtgttgga attctgtaat aagtataaag ccaaagcgct tatcgcttcc accagtgaaa
     5821 tttacggtaa aaatgaacag ataccctttt cggaaacaga tgacagatta ctgggttcaa
     5881 ctcatattag tcgttggggt tatggttgca gtaaagccat agatgaattt ctggcattag
     5941 cttattttcg ggaaaagaag ttgccggtag taattgtgcg ttgttttaat accgtaggtc
     6001 ctaggcagac aggacaatat gggatggttt tacccaaatt tattaaagct gctcttttaa
     6061 atcaaccgct ggtgatttat ggcacaggag aacaaacgcg ttgttttgct gatgtttcag
     6121 atgtagtgga tgcctttata aagctgatga acactcctga atgtgccggt gagattttta
     6181 atgtaggaac aacggaatcc atttctataa ctgatttggc acaaaaggtt aaagatatgt
     6241 gccacagcaa atcccgtatt gaatatatga gatatgagga tgcttttgaa gaaggttttg
     6301 aagatatgat gcataggcaa ccggatttaa caaaaattaa aaattttatt ggctgggagc
     6361 caaaacataa attggaccat atcataagta ggataattga ttactatgaa aagtaggttt
     6421 ttgctgattc ttttaatgag cttgagcttg ctttcggctc taacacctac cccaacggga
     6481 gatataatcc ctatttctgt gagtttaacg ggttttgtgg aaaaccccgg ggtctatcaa
     6541 atgactcctg taaaccgtct ttccgacctt ttattgctta ataaaacagc aacgctggag
     6601 cgcattgaaa aaacacaggt tttacgggaa aaaagtgttg aagttcctaa accggctgaa
     6661 ttattgagtc ctccaactcc tgaaaaagaa aaggaagaaa taataacttt ggagaataat
     6721 cagggtctgc gcagaattaa aataactcgt gccgggaaaa tagagaccta cgatttgttg
     6781 aaattttacc gcttgggtga tatcagtcag aatcctttgc tgaaagatgg cgatgtcgtt
     6841 tttgttccag ccatcaagga ttttatttca attggtggag gcataaatct gcctggcgaa
     6901 ttggaatttg tggaaggtga taaacttggc acaataattg atttatctct cggttttact
     6961 tttgatgccg atatttccaa agtccagctc tatcgctata aagaaaacag aattgattat
     7021 gatgttttaa attatgatct taaagccaat cctgcatttt gggatttacc cttaaaagcg
     7081 gatgaccgaa ttcttatttc ctgcgatgca gaaattagaa ccaggcaaag aattaaaatc
     7141 tacggtcagg ttaaaaaccc tggtgaatat gtgattgatg ccaataccac tttatatgat
     7201 gttctgcaac aggcaggtgg cttaaccaaa cgcggtgata taaaatcaat ggtctattac
     7261 aatgaaaata tcaatgccga tccagacccc tttctggaaa tgctgatgca gagaactatg
     7321 agtgaaatga cccctttgga atattcctat ttacgcaata atttaatgca actaaaaggc
     7381 aaatacagta tagaccccgt taaaatgata aatagcgaag gaaaagaagc aaatccttat
     7441 ctgcttgatg gtgatcgtat ttatattcct gaaaaaattg atatggtttg ggttagcggt
     7501 caggttaaaa atcctggtat ggttccctgg ttggaaggaa aggactggaa ttattatatt
     7561 caggcagccg gcggctatac taataatcgg aaaacaggaa agggcagaat tattcgtgcc
     7621 aacagtggaa attgggttaa gcccgggaaa aatgtagtta tcagagcagg tgatactgtt
     7681 tttgttcctg ctcaaacaga caggtctatg tggacagatg taaaagatat tgtaacgctt
     7741 acttcttctg tagttactat catccttggt attagaactt ttaccaggaa ctaaaggaga
     7801 aaaaatgaaa gttccgatgc tggatttgca agcacaatac gaacctctga tgcctaaaat
     7861 taaagaagca ctggaaaggg tttttgctga acataactat attatgggac ctcaggtgaa
     7921 agaatttgaa gagaagatgg caaattacct cgggattaaa aatgccatcg gttgtgcttc
     7981 cggaacagat gctctcgttt tagccgttaa agcattggga attggagcag gtgatgaagt
     8041 tattactact cctttcagtt tttttgccac tgcttcttct atctggcgca ataatgctac
     8101 tcctgttttt gtggatatag accctctcac tttcaatctt gatcccggta atattgaaaa
     8161 ggcaataaca gaaaagacca aagcaatttt gccggtgcat ctttttggtc aatgtgccaa
     8221 tatggattcc attatggcta ttgcccataa atacaatttg tatgttattg aagataacgc
     8281 acagggtatc ggttgcacct gggacggaaa aatgagctgt tcttttggag atataggaat
     8341 tctatccttt ttcccgtcta aaaatcttgg cgctatgggt gatgctggaa tgtgtttaac
     8401 caataatgat gactatgcat ttaaattgcg tcaattgaga gtccatggtg aaaatcccaa
     8461 atactatcat caatgggttg gtttaaacag tcgtttggat accttgcaag cagctgtttt
     8521 aagcgttaaa ctggactttc tggagggttg gagtaaaggc agacgcgcca atgctgaatt
     8581 ttacaatgaa cacttaaaaa atgttcccgg aatacggatt ccctatatag acccaaaggc
     8641 agtaagcatt tacaatcaat atacgcttgt ctgcgaaaaa agagatgaac tgatggcata
     8701 cttaaaagaa aaagaaatcg gctgtgccgt ctattatcct ctacctttac atttgcagga
     8761 atgtttcagt tcgcttggct ataaaaaagg tgatttgccg gtagcggaag aaatggctga
     8821 aaaagttctt tccattccaa tttatcctga gcttacaagg gaacagaaag aatatgtatg
     8881 taaaaccatt acaaactttt attgccattg aaggaaatag aaatatgcat tttattgtct
     8941 gtataaaaca agttcccgga actacggaaa tcaaaattga ccctaatacc aataccttaa
     9001 tccgagaagg tgtggaaagt attctgaacc cctttgatgc ttatgccata gaagaagcag
     9061 tccgcttaaa agaaaaatat ggcggaaccg ttactgccat cagtatgggt cccaatcaat
     9121 gtgaaacaac tttacgcgag gcagtttcct taggtgtgga taatattatt ttactatcag
     9181 ataggcgttt tgctggagcc gatacttatg ccacaagttt aactttagct gccgctatca
     9241 gaaaaatagg ggattatact cttattttaa cagggcaaca ggcaattgat ggagatactg
     9301 gtcaggtagg acctggcatt gcagctcatt tgaatattcc gcagacctgt tttgtccgta
     9361 aaattgaatc ctttaatgaa caacaggcag tagtagaacg cttgctggaa gatggttttg
     9421 accgtgtttc aatgcaactt ccggcgctaa tttccgtggt gaaagaaatc aatgttcccc
     9481 gtttaccttc tttaagagga aaacgaaatg ccaaaacagt tccgctaact gtttggaatt
     9541 gtgatgattt gggactggat gaaaaagaaa caggactaaa cggttctcca acacaggtgc
     9601 tgaatatttt ttctccccat cacgaaaagc aggtggaaaa gtttgaagga aacagtgatg
     9661 aggcagtgga actaattgtg caacgccttt cagaacttac taatagatga ttaaatatcc
     9721 cgaaataaac cccgatattg tcagttttaa cttaggaggg ctaaatctgc atattcgctg
     9781 gtatggcttt ttttatgtgc tgagttttat tcttgccttc attttataca agccgttctt
     9841 gaaaaagaga aacatcaagc ttacaaaaga gcaatatgaa tctattatct tttatgtaat
     9901 gctgggtgtt attttgggtg ggcgtttggg ttatgtgctt ttttacaatc ttggctacta
     9961 tatttctcat cccttattga tttttgccgt ttgggaaggc ggaatgagct ttcatggagg
    10021 tgccttaggg gtaattattg ctgggctgat ttattgtcat aagcataaat tgaattttta
    10081 cgctctggct gatgccgcta ttcctattgt agctataggt ttaggattgg gacgcttggg
    10141 caatttcatc aatggtgaat tatggggaaa accgacagat ttaccttggg gaatgatttt
    10201 tcccggggca gataatttac cgcgccatcc tactcagctc tatgaacttt ttctggaagg
    10261 cattgtaatg ttcattgtca cctattatct gttgaaaaag ctgaaaaaag aagggctggt
    10321 tttctggagt tttattggtc tctatggtgt tttcaggttt ctaattgaat tcgtgcgtga
    10381 gccggatgat cttgattttt acacccgttt tggttacatt ttcggcttta tgagcattgg
    10441 tcaattctta agcttaataa tgattattgt ggctatctgg ggtttttaca aactcaatgc
    10501 tccgcataaa aaggaaaaga aaccgaaaat ataagttagt ttgcctgtat atgggaaatg
    10561 gttattttga tttggattcc tgcttttgcg ggaaggacaa aggacttttc ttgccaataa
    10621 taacataagt ccctttacaa gagaaatttg gcaaaccgag aaaacgattg aggttaataa
    10681 tgaagaaagc attaatatcc gttctaatta tctccgctat tatcatttct gcctgtgcag
    10741 gaccaaaaga taaaagcgaa gaagaaaaat tacgccagca gctgaaaaaa tgggaaagtt
    10801 ttgatagtga aggcatagca gaggtctcgt ataaaagttt ggctatccgt aaaatgttta
    10861 gtgctgccaa aaacggtagt gaattacgct ttgacctttt tgatggcggg cttttaggtt
    10921 cggcaggaga acctttactt actatgtatc ttagggatta tgttgccttg aaatcacctt
    10981 ttataccgat gctggaactt ttagatttaa caccttccgc tcctttacag agcttaaaat
    11041 tatctgctaa tgccgattca cttgttgctc tttacggcga gaggattatt cgggataaga
    11101 aactggagct gaatggagtt actatcaatt tcaacaagga ctacctttta gacagtgttt
    11161 gggaaccaga aaacaagacc caaatgaagg cattatacaa tagcaaagga gacctttccg
    11221 aattagttgt taccagtata gacaacattt ctcttagaat gagttttgat aaaattgaat
    11281 atgttcagcc ccagattatt cctctgccga aacctgagct ttccttttcg gaagatgcct
    11341 tgaaaggatt aaacaactta aatctggaat cactgctgaa ggactttctg caatataaaa
    11401 aggagaggca aaaatgattc cgcgttattc taaacctgaa atggaaagaa tctggacttt
    11461 ggaaaaccgt tacgaatgct ggctggaagt ggagcttgca gccgccagag caatgtttga
    11521 aaaaggcatt attcctaaag aggattggga gacaattaac actaaagccg attttgatac
    11581 gaaccgcatt gacgaactgg agcagattac caagcacgat gtaattgctt ttttaaccaa
    11641 tgtaggggaa aatataggtc ctgcctccag gtggctgcat tttggaatga cctcttccga
    11701 tgttttggat actgctacag ggatgcaatt aaaaagcagt ggagagctta ttttaacgga
    11761 aataagtcgg ttggcggaaa ttctgaaatt gaaagccagg caatataaaa acacaatttg
    11821 tatgggtcgt tctcatggaa ttcacgcaga acctacatct ttcggcttaa agtttgccct
    11881 ttggtatgaa gaaatgcagc ggaatataaa acgcttaaat gaagcaatag aagaaattag
    11941 cgttggtaaa ttttccggtg cggtaggcaa ttttgcgcat ttaagcccgg aaattgaaga
    12001 acttgcctgt aaatatctgg atttgcatcc ggcaaatgtt tccacgcaaa ttatacaaag
    12061 ggatagatat gcctttttcc tttcggtttt agcactaatt gcttccggca tagaaaaaat
    12121 cgctttggaa ataagacatt tacaaagaac cgaagtgcac gaagtggaag agaacttttc
    12181 caaagggcaa aagggttctt ctgcaatgcc ccataagcgc aatcctattg ttagtgaaca
    12241 gctttgcggt ttggcaagaa ttttgcgttc caatgcctta agcgctatag aaaataatgc
    12301 cttgtggcat gaacgcgata tttctcattc cagtgtggag cgcatcattt tgcctgattc
    12361 ttgcatttta attcactata tgctggatag aacctgttct ctgatagaaa atctgatagt
    12421 ttatcccgaa aatatgcaaa aaaacctgga actgactaaa ggtcttgttt tttcgcaagc
    12481 attgctgttg catcttgtcc aaagcggttt aacccgggaa aatgcctatt cctttgttca
    12541 gcaggaagca atgaaatcct gggaaacaga cagtagtttt ttggataatg ttttggcaaa
    12601 taaggaaata acttctgtcc ttgcggaaag cgaaattcgg gatatatttt cttatgaacg
    12661 ctatcttcgg aatgtggatt atatttacag gcgttgcgga attttataat ccgggagcaa
    12721 aaaaatgaag aaatacttaa ttatcgggct ggctgtggtt ttatgccttc ctgccttcct
    12781 tttttgcaaa gaggaaacaa accgccttat cggctactgg ctaactggtg ataaaacaat
    12841 gaaaatagag atttacgaca atggagacag cacttttgcc ggtaagatta tttggctgaa
    12901 agagcccaac gacaaaaacg gcaaacctaa aatggacacc aaaaatccgg atgaaaaact
    12961 gcggacgaga cctttgctgg aaatggttat tttaaccgga ctcattcccg aaagcaatac
    13021 caaatataaa tctggtaaac tttatgaccc caatagcgga aaaacatatt ccgccactat
    13081 tgaattagta aataataaca ctattaatat ccgttacttc attgctacac ctactttagg
    13141 cagaaccgat acctggactc gcacttcccg ctaattttta agcaggataa gtaattatct
    13201 ggctggatgt aatctacacc aagatcagaa cagagggcaa agttaatttt actgtggtgt
    13261 taatagcgat tggtctcaaa gaaaatggtt accgtgatgt tctgggtctg cacttgggga
    13321 acaaggaaag ctattatata ttttccatta tgatagtata tatatagtaa aagagaagtg
    13381 actgtcttat aaattttagc ttgtaaattt aaaagcccgc agggcgactc cgagcttttt
    13441 ttcattagcg agctaaaagt tataggacaa acattttact tttcctttat atgccctatc
    13501 aaaatggaat atatataagt cctatcccac atgataccag aataatttga aatcaatgat
    13561 taaaatgtct gaacttgctt aaagataaaa tccctgaata taatccgggg ttcttttcta
    13621 taatttcagg gttataggaa taatttactt cataagcagc attttcttgg tctcaactct
    13681 cccattactc tctattttat agcagtaaac tcctgaagaa cagattttac cattattatc
    13741 cttcccatcc cagaccatac tatgtttacc attgctcatt ttattgttga taagtcgctt
    13801 taccagctct cctttgaggt tgtagatatt gagatctaca ttgccaggtt ttgataactc
    13861 aaaagaaata gtagtttcag gattgaaagg attgggataa ttttttaaat taaatgtaat
    13921 aggaggtaac tgaatagaat cttctgcctc cattacctga ttcaggtcca gaatatttaa
    13981 accataaagt ccattagcgg tatataaata gccattttgt attaccatat cttctgtgga
    14041 taaattccag ctatatctac aaacaaattc aggagcagca ggattactaa tattataaaa
    14101 atataattca tcccagttag tatcggaaat aatcaacaga ttattgaaga ttatgcattt
    14161 gtaaatataa ctgcttggac gaggaaggat agtatttaat agtattggat tttgaggatt
    14221 tgaaacatca ataatctgta aaccagaatt cccatctgct atatatgcaa tatcatttga
    14281 aacaaaaaca gagtatgaat tagtaggtgt atcataagag cccagcagat atgggttatg
    14341 aggattagaa acatctataa tcagcagacc ctcatagtaa tctgctacat aggcaatatt
    14401 attagaaata gtcacaaaaa gtgctggtcc tggtgtattg taggaaccaa gaagcactgg
    14461 attatgaaga ttggaaacat ctataatctg taaaccagaa ttcccatctg ctacataagc
    14521 aatattattt gaaacaaaaa tagaggatgc aaaactaggt gtaacataag agcacagcag
    14581 agatgggttc tgaggattag agacatctat aatttgtaaa cctgcccaat aatctgctat
    14641 atatgcaata ttatttgata caaaaacaga gtatgcataa ccaggcgtat tataatagcc
    14701 cagcagagat aggttctgag gattggaaac atctataatt tgtagacccg aatcgtcatc
    14761 tgctatatag gcaatattat tagaaacaaa aacagagtat gcataataag gtgtatcata
    14821 ataacccaca agagccggat tctgaggatt ggaaacattt ataatctgta aacctgccac
    14881 ataatctgct acataagcaa tattattaga aacaaaaata gattctgcag aacctggtgt
    14941 atcataagaa cacacaagag ctggattttc aggattggaa acatttataa tctgtaaacc
    15001 cgtatcgcaa tctgctacat aagcaatatt atttgaaaca gtaacagatt ctgcatgacc
    15061 tggtgtatca taagagccca gcagagatgg gtactgagga ttggaaacat ctataatctg
    15121 taaaccagaa taatcatttg ctatatatgc aatattattt gaaacagtaa catatcttgc
    15181 actaccgagt gtaccataat agccaaaaag agccggattt tgagggttgg aaacatctat
    15241 aatccgtaaa cccccatagt aatctgctat atatgcaata ttattagaaa caaaaacaga
    15301 gtattgataa ccaagtgtat cataataacc cagcagagct ggattctgaa gattggaaac
    15361 atctataatc tgtaaacccg attccccatc tgctacatag acaatattgt ttgaaacaaa
    15421 aatagagttt gcagaacctg gtgtatcata ttccccaatt ttgtgataaa cagcacttaa
    15481 gggtaatata attattacca caattaaaaa taaaaccatc tttttcattt ttctcacctc
    15541 caattttatg aaattacctt taaatctctt ttcatttttt ggtcaagaaa aagaaaaata
    15601 ttttcataat atgacaacaa agctttttaa tatcgcaccg agagcaccga gagcaccgaa
    15661 aacctctcac agattacaca gatttcacag attataccgt taagtgagga gtcatttgtg
    15721 ccttgctaac ttttgaacct gttgataacc ttttaggcac ttatgacata tcacgaggtt
    15781 aacaattccc tcacggatag ttaccgccgt cattagtcct cactgccaaa caagttatcc
    15841 aaaatccaga ttgataaatc agcaatgaat agaaaatctg tgaaaaagct atataatcag
    15901 aaaataactt tgaaacaaca aaaaagccct tgagggcgaa acagttatag cgatgggtgc
    15961 gtaagcccct cgtaagttta aacatataga agtttttaaa ccttttctaa actttggtag
    16021 agcccccaag ggcgacactt ctattttgaa cttgttttgg ctcttgggaa aattttaagt
    16081 gtcgccctga ggggcttttt tggacaaggt tttgagaaat aaattaataa tgatattact
    16141 ttccccgagg ggtttacacc accatcgcta tcattgtttc gctctccgag cttttaaatt
    16201 aaagggaaaa tatattatag ggatgtttat aataatgaat ggcagtgatg actcctgact
    16261 taagcagttg ttcggttcta aattacaagg atatttagag gattggataa gcggaaaact
    16321 gtatctttca gagctattgg aaaggatccg ggaaaacttg aaaaagccga taccttttga
    16381 ggcaatccat cgcgacaagg tagcttttga tgaaaaaaac ttgacgagaa ttgacaaaat
    16441 gaaaagaatg taatttgtta cttgtctata atatagattg tgagaaacga gggataacag
    16501 atgccctcaa agaacctgag aaacaacaag ttggaattta caccaacatt tggggctaaa
    16561 cttaatttga ggtattgctg aacagtatgg acagaatgcc tacatagatg aacagaagta
    16621 aactaaaagc gctaaaaaaa ggagttaaaa tgaaaagagc ttatttgatt ataccctttg
    16681 ccattatttt attgctgctt acctcttgtg ctacaacagg cgaagagcag gcaaccaaac
    16741 ttgttaccaa atatgacatt gcccttacga aagttagtag aggcggagat gaaactacac
    16801 gaagtaaagc tcctgtggct gatactaccc tagtagataa attgcgttac cattatgatg
    16861 acgaatttat gcacactatc tggtatgctt ccgaaaatgg ctttgaactg accttataca
    16921 atgcttcgga taagaatatt attctggact gggaaaaggg tttatatctg gattatgaca
    16981 atataggcca tcgcttgttg atttccagca cccctgattc ggaaaaagat aaaccccaat
    17041 cacccagcac aatacctccc cgttctaata ttacggaaat tatctattct gccgatcata
    17101 ccaatccttc aaccctttta ggtgtttatg tgcgcactcc tcttctgcca acggaataca
    17161 aaaaagcagt ccgattcaac ggtaaggaaa tgaaaatcca gcttcccgtg agaattgata
    17221 ataccaccta taactatgaa tttactttca agattgttgg agtgcgtcag gaacaggcaa
    17281 aaagcaatct gctattcgga taattagtta agcttcccta ttattaaagg ttggcaataa
    17341 agacaaggtg gacttgtaga gtcctttttg gcaagtataa tttgattttc caagaagcat
    17401 tgcttaccca ttgcttaccc aatactaatg ccatccgtaa tgggtaagca atgggtaagt
    17461 aatgctccca agtaagaaac tgtattttct tctcttattg taagataaca aacaaaataa
    17521 aatatttcgg gagccatctc tgccaaaata acttctacac tacctgcaaa ttctttgtgc
    17581 agtgagaact cataacgctt tattgggtaa acggaggata ccattcctct gccaaaaatt
    17641 atgcaaagct tttgctacct ccgactacac aaaaaaaaca ggattgcgct tttcagcgca
    17701 accctgtaat ttgttacacc ataatagatt attcaatggt gatttctttt ttgggagagg
    17761 gctctttctt cggaattata actttcaaaa ccccatcttc catcttggct gagattttgg
    17821 aaacctcaac attctcagga agtaagaggt tacggcgata gcttccactg tatctttcac
    17881 agcggtaaac tgtgcctttg gtttcttctt tagtaacatt actattagct tcaatcatta
    17941 attgattatc atgaatgctg attttaacat cgtctttctt aaatccgggc agatttgcca
    18001 gaatctcaaa gtccttatca tgctccacaa tatcaattgc catagcccgg aagttatctt
    18061 cctctccttc ttcttcgtaa aaacgattga agaactcgtc aaataagctg agcatattgc
    18121 tcaccggtct cagttcgttt gttttgcgat atggtacgat tttcattttg aacctccttg
    18181 ttcattttat cgttcactaa ttattataat ccagtttttc caaaaagcaa gaaaaaaatt
    18241 agcagtcaaa gtgcaagagt gctaatttcc ttttatacaa agaaatttta gacactgaaa
    18301 acaccgaaat tttttttacc aagagaaaaa gagaaaaagt tcagacaccg aaaacgccga
    18361 aaacactgag aacaccgaga tttttttacc aagagaaaaa agattggatt taatacctgt
    18421 gttaaattaa atatagcttt gtccttcctg cgctcttgtc cctccggtac aagcaggaat
    18481 ccacagatga aaaaaaatat ttatcccttt tcaagttgaa aacgatattt gttcttaaga
    18541 gaacaaagcg caagtcgctc ctctgtttat ataacccagc tgtttaaaaa ggataattga
    18601 caaaaaggca aggcaggtat ttttggactt cagttttccg cttcccggaa atgcagttta
    18661 acttgataca aattaaggaa atagaatgaa agaagagttt atccgcaatt tctgcattgt
    18721 ggcacatatt gatcacggaa aatctaccct ggcagataga tttttggaag agacacatat
    18781 tatcggtaaa ggaacagagg tctctcagat tttggacagt atggatttgg aacgcgaaaa
    18841 aggcatcaca attaaaagtc atgctgtccg aatggttcat aattatcaag gtcaggatta
    18901 tgttttgaac ctgatagata ctcctggtca tgtggatttt tcttatgagg tttcccgtgc
    18961 tttggcatcc tgtgaaggag ccattctttt agtagatgcc tctcaaggaa ttgaggcaca
    19021 aacaatgagc aatctgtatc ttgcattaga taacaacctg gagattttac ccgtaattaa
    19081 taaaattgac ttacccaagg cagatattga aggcacggaa aacgatttat gcgaaatttt
    19141 aggttgccta cccgaagaaa taattaaggt tagtgctaaa acaggtgaag gagtaaaaga
    19201 ccttttagat gccatcgtaa ctcgtttacc agctcctaaa ggcaatcctg acacggctcc
    19261 cagagcattg atttttgatt cctattttga tatgtatcgg ggtgttgttg ttttagtgcg
    19321 tgtttttgag gggaaattac aaaaaggcga taaaataaag ctcttttcta cggcaagaga
    19381 atatgaaatt gaagaaatcg gctatctggg cttaaaattt tctccccaga cagaacttac
    19441 ctgtggtgaa gccggttata taattgctaa tatcaaagaa gttgccgatg ccagagtggg
    19501 tgatacaata actttaagca aagggggttg tgaaaatcct cttcctggtt tcagagaacc
    19561 caaaccaatg gtctatagca gtatttttcc tatcaatgga gaggattatg aaaatttggt
    19621 ggaatccatt gccaagctga aattaaacga tgcttcccta atttatgaaa aagaaagttc
    19681 tgccgctttg ggatatggat tccgttgcgg ttttttgggt atgctgcatc tggaaattgt
    19741 gaaagagcgt ctgttgcgtg aatataatat tcccattatg gctacaactc ccagtgtgcg
    19801 atttttaata aaactgaaaa gcggagaaga actgcaaatc aataatccgg ttgattttcc
    19861 tgatcccagc actattgaaa gtattatgga accctttatg gatacggaaa ttattgtgcc
    19921 tacggattac attgggaatg tgttaaagtt agttcaggaa cgcagaggaa ttcaaaaaga
    19981 cattcagtat atagatgaaa aaagagttac tctgcattat gagctgcctt taattgaaat
    20041 tatctttgat ttttacgata aactgaaaac cgttagtcgg ggttatgctt ctttggatta
    20101 tacttttaag gactatcggg aaacagatgt agtgaaagtg gatatcttaa taaacggtga
    20161 aaaggttgat gcaatgagct ttatctgcca tcaggataag gcgtataatt ggggaaaaag
    20221 tgtaactgaa accctggcgg aagttattcc gaaacatctt tttaagattg ctctgcaggc
    20281 agctattgga ggaaaaatta ttgcccgcag cactatcaat cctttgcgga aagatgtttt
    20341 agcaaaatgc tacggaggtg atgtttcccg caaaagaaag ctgctggaaa aacaaaaaga
    20401 gggcaaaaaa aagatgaagg aaattggttc ggtttccgtt ccccaagagg cattcctggc
    20461 tgtgttaaaa gctgataggg aataaagggt ggtctttttt cagagcaggc attgtttttt
    20521 aacactcttt ttctgccttt cttttgggct tattttagct ataaatattg gaaacataag
    20581 ctttcaaggc atttccggaa tagaagaaac agaacttttt aaggcaagcg gtctgcaaaa
    20641 aggacaggaa tacaacccgg aacttatttc caatgccact tctaacttgt ataaatattt
    20701 gcagaataaa ggcagatact ttgtttggat ttcctctccg gaattgattc cggaagatga
    20761 aggaactgtt tccctgctct ttaagatgca agaaaaagca ccttccgatg aagtaaaaat
    20821 ccgctttcag ggtatgcagt atttcagtga ggcaaaactc tttcaacttc tactgctttc
    20881 accggaaaga aattatcact taaaccagct ttcccgaatt atgcaacaaa ccctttcgct
    20941 ttatcaaagc aggggttatc tctttaccaa agttgaattg gattctttgg tttgggaaga
    21001 ggcattgagc gcttatatcg gaattgatga aggcaaaccg ttacgcgtga aaaattatat
    21061 ctttaaagga aataaagtta caaaggacaa aaccctgctg catctttccg gcttggaaaa
    21121 tattgacttg attactcccg aggtcttaac tcaagcagaa gaaaatatcc agcgaaagag
    21181 ttatatccgc aattgtaatg tggaacccgt aaatgaaaat accttgctga taaacattga
    21241 agaaggtaaa atgacttatc tggaaggcgt tttaggaatg aatcggatta acgaggaact
    21301 gaaattatca ggtcaaatcc gtttgcaatt cttgaacctg tggggtaccg accgtgccat
    21361 aaaacttttc tggaaacaga ttcctacttc caatagcgaa ctttctttgg cgtatcacga
    21421 atcaggtatt ccgggaattc ccattgcagg agatattgaa ctctatagag ctcaacagga
    21481 ttctacctgg ataaaaactc gcggtagctt agaaatttat tatcaaatgc tgaaacacag
    21541 cttaggtatt gaactggcaa cggaatcctt aaatcccggt gcaagaagac ccgctattat
    21601 aaataaggaa aaggcacaaa gtataggcgc tttttggaat tatagcaatt acgcaggtgg
    21661 cgtaaatcct tataaaggta tggaaatgaa atttctttac cgtttaaccg gaagcaatct
    21721 gacgaaacat ttatatggag ctacagaagc aggcgtgaag ggctatattc ctgttaaaaa
    21781 tcgctttgtg ggttttatag gtgcggaaat tcgtaatctg gaaaacaaaa atgcagaact
    21841 ctggcaacaa tataaaatgg ggggctatgg aactttgcgt ggctattatg aagatgaatt
    21901 cagctctttt cgccttgcct ggataaatta tgaattacgc tatcgcctta atcctgattc
    21961 gcgtatttat ctcctctttg atcaggggtt tttaggcaag gaaaaaaaca agttaaagac
    22021 cgatcttttt ggtatcggtt tcggaatgaa aataaataca cgcttaggaa ttttaggatt
    22081 ggaatatgct cttggttata gggataaaca ctttgccaat cttggttccg ggatgattca
    22141 tctgggcttg gatgtggcgt tctaaaaaaa atacttgcca agattttggt atcctaaaat
    22201 gatgtcttca ataactaagt atatagatgt gactaaggac tgtgtgaagg atagatgaga
    22261 gttttttaac tctatcaggc cttcaataga accggcaaat ttttatatgt cttgccaaaa
    22321 caaatcaaaa aaaagaaaaa aacctaagta cccgaaaggg aatttgctta tggaggaatc
    22381 aatgaagaaa gttctcttaa tcaccttgct cgctatgttg ctgatcagca cattggttac
    22441 tggctgcaaa aagaaagcag aagagcccca ggaagaagta gctcctattg aagaaactac
    22501 tcctgctcct gatacaacag tagctcctac agagggccaa acacctacta ctacaccaac
    22561 cggtaagtag tttatcagct aataaacaat agcgcagtcc ttaaaaaaac gggtgtcttc
    22621 ggatgcccgt tttttgtttt aagctgattt ttagccaccg aaagcaccga aagatttaag
    22681 caagaagatt acgcagattt ttagccaccg aaaacaccga aaacaccgaa agatttaagc
    22741 acacagattt ttagccaccg aaaacaccga aaacaccgaa agatttaagc acgcagatta
    22801 cgcagatttt tagccaccga aagcaccgaa agcaccgaaa tttatatata attataacca
    22861 ataatattag ttcgtagttc tgggttcgta gttttgggtt cgtagttctg agttcgtagt
    22921 tcgtagttgc ttattataac ttatattttt ctctttttct ctttttctct ttgtgaaaat
    22981 caactctttt tctctttgta aaagtaatct ctttttctct ttttctcttt tctctttgta
    23041 aaagtaatct ctttttccct tgacaaattt agtagcacta ataatgaagg tttaagatgt
    23101 taatattact catttctaag tgtagctaac atcggaagga gataattatg attggagaac
    23161 gacttaaaca gattcgtcaa gtattgggaa tcaaacaggt tgatttagcc aaggtcttaa
    23221 agattaatcc ttccgctatt tctcaaatgg aaagtggcag aaccaatcct tctttggaaa
    23281 cgctttcgga attggtagta aactataatg tgaatctgca ctggttaata acgggtatag
    23341 gaaaaatgtt taatactgct aatgatacat cttctcagaa tggttcttgg gataattttc
    23401 agaagctctt aaatgaccgt ttggaagaaa ttgtgcaggc acgcttggat ttaatggatt
    23461 ccgacacggt agaaattccg gttagtggag aaattgcagc gggtgaacca atggaaaatt
    23521 atggtacttt actggatgtt gtaacagtgc gtcgttcctt gataaatggt tctttgaata
    23581 attttgtggc tttaagagtg aatggacgta gtatggaacc ggatattaga aatcaggatg
    23641 tagtattaat ccgttattgt aatgaatggc gagagctagc aggcaagatt tgtgcggtgc
    23701 gtattgatgg aggaataaca ttaaagcgtt tgattttgga tgatgttcaa aagatgatcg
    23761 tattgctttc cataaatgaa aattatcagc cgattctgat tgatccggat agtcatacag
    23821 atgttacact aatcggttct ttatattttt tattcaggat attaccttaa ggagggataa
    23881 gtaaatgact ttagtttatc ttcttattat tgtagccatc gtctttgcta ttgtaaattt
    23941 atatatttta ctttctcaac ggcataaagg caaggaaaat ggaaatatca gtgaattgaa
    24001 gggtgccttt gagacctttg aacgctataa tcgtgaagaa atgagcagtt tacgaactga
    24061 acttttaagt atcagtgctg aaaaccgaaa agaacttagt gactctatta aagctcaaaa
    24121 tgaggtttta ataaaacaaa gcactgccag cagggaagaa ctgaataatt cctttaatac
    24181 cttaaaacaa cagattaata acgattctgc cgttaatcgg gaagaacaga aaaaagcatt
    24241 gaacgacctt tccgagaatt ttacccgcag aatgacagac ctgataaata ttcaacaaca
    24301 ggaagcggaa acatcgcgta agacattgga aactaaaatg gaacaaattc gtcaaaataa
    24361 cgagaccaaa ctggaacaaa tgcgtcaaac ggtagatgaa aaattgcacg ataccctgga
    24421 aaagcgttta ggggaaagtt ttaaacaggt tagtgagcgt ttggagctgg tgcataaaag
    24481 tatgggtgaa atgcaaagtt tggcaaatgg agtgggagac ctcaaaaaag cccttaccaa
    24541 tgtaaaaagg cagggagttt taggggaaat ccaattagcg aatttactgg aagacattct
    24601 tactccggag cagtatgaga aaaacttccg acctaataaa aaccgtgatg aaaaagtaga
    24661 atttgccatt cgcctgccag gaagttcgga aaaagaggaa tttgtatatc tgcctataga
    24721 tgccaagttt cctatagcgg actatcaaaa tattatgaat gcttatgata tgggtgattt
    24781 ggcggaagtg gaaaatgcca gaaaaagtat ggtgcaaagg attaaaaatt gtgccaaaga
    24841 tatccgggat aaatatatta atccacctgt tactactgat tttggaatgc tttttctacc
    24901 ttttgaaggt ctttatgccg aggtcttacg cagtccggga ctttttgaga caattatgcg
    24961 agattatcat gttatcattt gcggtccaac taccactgct gcggtaatta acagtttgca
    25021 ggtgggtttt cggaccttgg caattcagaa gaagacctca gaggtttgga aaatccttgc
    25081 ctccatcaaa ctggaatttg ggaaatttgg aactattttg gagcaaaccc aaaagaaact
    25141 gcaagaggca tcaaataata ttgaaaaagc aagttaccgc agccgtcaaa tagaaaaacg
    25201 actggataag gttcaggaat tgcctgtgga agaggcagaa atagaacaaa ttctggatgt
    25261 ttgatcgccg gaagaacgac atcctgtcat tctatttttt cttttataag ttgacaagga
    25321 catcaacttt ccgggcaaac aaggttgacg gaaaacatca accttccaat accgccaaac
    25381 aaataccttc cgttttgata attccggctg acgaagcagt tataatatta ctgattccgc
    25441 gtgattgata gtcccttatt acgaggtcgt tcaggtgata tttcaagttt tgggaccagg
    25501 aaaaaacaac tttttcggag agtgggataa gagaaagcaa ttttccctgg caattgttaa
    25561 attctatgct atccggcaga ataaaaatgg actggttttc actttcaatc ctataaggaa
    25621 cagggtagga tattctttcg cctcttggac tttttatttt taacaaattt atggcaatag
    25681 ccaaggcgtg atcaaaacga ccttgcatat cgttgcagat aattatttcc cgatagcatt
    25741 gttgctttaa acaccaatcc agtgccagtt cagtatccgt ttcgtttttt tccgtaggat
    25801 gcttgtggaa ggggatgtaa gcatattttt caagcatttc gggttcaatg gaatccaggt
    25861 ctccaatcag aacaaagggc tctaatccaa gctcatcaat tttttttatg ccgttatcaa
    25921 cagcgattaa atggtctttt ctttggatat tttgatagcg ggaaacaata tcttggggag
    25981 cgtgattggt aaaaatataa gcgcggttat tattaacgca tagcatagag aggttcatct
    26041 attcggttca aataagcaac aggatttact tttcccagat tattatgcac ttcataatgc
    26101 aaatgaggac ctgtagaaat tcccgaactg cccatcagac ctataatttg accttttata
    26161 acttgatctc cggaacgcac catatagctg tagagatgac cgtaaagcgt ttgatatcca
    26221 ttttcgtgat tgattattat ccttttacca taactcgttt cataatcggt agttaccaca
    26281 gttccggctg cagtggcata aattggagtt cctgcctcat tggaaatatc aagaccataa
    26341 tggaattcaa tttcattccg aataggatga actcgcaaac cccagccgtc tgaaattctg
    26401 ccgaaagtag gataaataga aggtatataa tcggcaggat tttcccctgt aggcaaagca
    26461 ggaattgctt gcaccggttc tgaaatcagc accagaattg tagccagctt tgtttccagg
    26521 ttttccatct gattgcgcaa agccggttca aaaacaaagt ccgccttttt tccctgctta
    26581 aaattcaggg caggatagtc ctctgagttc tcattaattt ttatttgtaa ttcatttaat
    26641 ctcttataga tagaatctac agtagcggag taaaaatcaa gtttggcacg tagttcctta
    26701 ttttccgttt ccaaccgctt aagaacttcg ttacgatgag aattgctacc tgccattgtg
    26761 aacataataa ttgtggaaac aagcaaaaat gccaaaagca caattatcaa aataaaggca
    26821 taatgaggca gggtgaagct gtgtttaact ccatccattc caacttgata gcttattttc
    26881 attttacctt tgttgataaa ttcgttttcc actggcgtcc ttttctctta tcttttttct
    26941 ttctatatta tgcaaaatgc gaaccaaaaa tggttatttg gcatcccgta ggggaatcga
    27001 acccctgttg cgtgactgag aatcacgagt cctaggccac tagacgaacg ggacacaaat
    27061 tgtttcctta tttttaccct ataaattggc gacccctagg ggaattgaac ccctcttgca
    27121 agaatgaaaa tcttgagtcc taaccaatag acgaaggggt cgcaaactgg tgatccaggt
    27181 tggaatcgaa ccaacgactc tctgcttaaa aggcagatac tctaccgctg agttactgga
    27241 ccttttttct gcttatcttt taactaaaaa tttagaggtg ggttctatgt caagtaaaat
    27301 cgtatttatt ttttaacacc gagatcactg aaggcattat gcgttctcac ttattttttc
    27361 aaatcggcgt ttttgctggc ttgcgatatc acgatttttt caaaacgata tcaggattat
    27421 ttcaaatatt tttagtatgg cattgtatga cttgcgatta tagccggcac tctttttatt
    27481 tttccaagaa gaaattccgt aggaacgacg ggttttaacc cagcgttatg aagttggaag
    27541 atatcttatt tcctttttgc gaatgagttc cgtagtaaag tggaagttgc taatgcattt
    27601 gttctcagct gcattttggg ggttaagtaa agtccattgt tttcagggca tctcagcttt
    27661 ttcccagctt gaattgctga agcattgctt acccattacg gatgggatta gcaatgggta
    27721 agcaatgagt aagcaatgct attaaggaag aaaatggaat tatatttatt tagtaggagt
    27781 taggaaaatt tagggggaag aagaaaatat tatatctgta actccatatt ttatatagtt
    27841 ttaatattca taaactctat ataataaaat aaatcctgta gtgatggcag ttagataacg
    27901 acgggtttta accccgacgc atattctatt ttaactcaca attttcactt gccctccagg
    27961 tacacacatc ccacaaacat cttcatttgc aaccgagtta taacttgttt acgaattaaa
    28021 aaattgtgag ttaaaacaag aacctgcaca ctgtcaaccg aactacattt ggtctcctca
    28081 tttcaaaatt gtgagttaaa acaagaaccc tgcacactgt caaccgagct acagcattcc
    28141 gtcaaattcc acggtcacgg aaaaaatata tattcagccg ctccagaaat gtggggtagg
    28201 aattgttcag aatttgtgac ttcggggaat actttcgttt taactctaaa ccgagcttca
    28261 gctttgaaag tagaaggcaa cgaacaaaat ttcctattac ctcagaatat agttgcaaac
    28321 acaacagggt cggtttagag tcaaaactcc cccctctact tgttttgact cgcaattttc
    28381 acttgccctc caggtacaca catcccacaa acatcttcat ttgcaaccga gttataactt
    28441 gtttacgaat taaaaaattg tgagttaaaa caagaaccct gcacactgtc aaccgaacta
    28501 catttggtct cctcatttca aaattgtgag ttaaaacaag aaccctgcac actgtcaacc
    28561 gagctacagc attccgtcaa attccacggt cacggaaaaa atatatattc agccgctcca
    28621 gaaatgtggg gtaggaattg ttcagaattt gtgacttcgg ggaatacttt cgttttaact
    28681 ctaaaccgag cttcagcttt gaaagtagaa ggcaacgaac aaaatttcct attacctcag
    28741 aatatagttg caaacacaaa agggtcggtt tagagtcaaa actcccccct ctgcttgttt
    28801 tgacttacaa ttttcattcg cttacccaat tatcagcatc cctcttcctt ctacacttcc
    28861 aactgcacta caaatggttt cccaatctaa aaattgtgag ttaaaacaag aacctgcaca
    28921 ctgtcaaccg aactacattt ggtctcctca tttcaaaatt gtgagttaaa acaagaaccc
    28981 tgcacactgt caaccgaact acaacattcc ctcaaattct acggtcacgg aaaaaatata
    29041 tattcagccg ctccagaaat gtggggtagg aattgttcag aatttgtgac ttcggggaat
    29101 actttcgttt taactctaaa ccgagcttca gctttgaaag tagaaggcaa cgaacaaaat
    29161 ttcctgttac ctcagaatat aattgcaaac acaaaagggt cggtttagag tcaaaacaag
    29221 aacccactat ttcgctattt tacaatttga tgtaggaatt accgctgctt ttagctatca
    29281 gtcctttcaa ttccaaattc agcagtaaag tagaaagttt tccaaaaccg agacccgttt
    29341 tcagcataaa ttcatcaaaa gtaagttctt tttgtgcaga agacaaaata tcatagaggg
    29401 tctgttcttc ggatgacaat tccggaataa gttccatttg ttcttttgga gaaggaacta
    29461 agcccaaatc cattaagagg tcattggcag aactgattaa tgaagcaccc tgtctgatga
    29521 gcaaattagg accttgggca ttgcggttat tgatatttcc tggcaaagca tagagcggtt
    29581 tattttgttc aatggcaaat ttggcagtga gcatagctcc gctatttaaa gagccctcaa
    29641 caactaaaac ggcattggca agagcgctta ttattctatt acgggtagga aaattccagc
    29701 gttccagttt gcttccgggt tcatattcac taaccagagc tccgttttcg catatatttt
    29761 ctgcaagtgt tttattcata gggggataga tattatccaa gccggaggca aaaacggcaa
    29821 ttgttttggc ttttgctttc agagctgtct gatgagcaat ggtatcaatt cccatagcta
    29881 agccactgac aacattcact ccttttgcgc ataagggaga aaggagttta cgacacattt
    29941 ccagtccgta ggaagttggt ttacgagttc ctacaactgc gatacaaagg tctttcaagg
    30001 ttcccgtaag gtctccttgg taatacaaaa tcaaaggtgg atcaaatatt tcttttaagg
    30061 cagcaggata atcaggatcg gttaaacaga gatattgaag ctgatgtttt tccattaaag
    30121 ttaaaattcg tttgttgtct tcgggcaggg taaattcgct taagtgcttg attgtggggg
    30181 gttttagttt gccattttga taaagcggat gagagggatt acccacaaat tcttgaggat
    30241 caggaaaact ctgtaaaagt tccagcgttc cttttgtgcc taaaagggga gctgatttca
    30301 agcagagcca tgctttatat cgttccatct aacggctctg ctgtaatata ttgtaaattt
    30361 tgtatttaag ttcatctaaa ccgtctttgc tgactgcgga aatggcaaaa atttcatcat
    30421 tatattcctt tttgaaggca ttgcgtattt ccgtcaattt ctgctttaag tcctcgggag
    30481 aaagagtatc tgttttgctg ataacaatta aatggggttt tttatccata aaggaatcat
    30541 aaagatacag ctccgacctc aaagttctgt atgcttccaa aggatcggga gtggcaatat
    30601 caattaaaaa aagcaagaga tgagttcgct gaatatgacg caagaattga tctcccaatc
    30661 ccttacctaa atgcgctcct ttaattattc ccggaatatc agccatcaca aaattctgat
    30721 aatcactaat ataaacaacc cccagcatcg gttccaaagt ggtaaattca tagtccgcta
    30781 ttttaggacg agctgaagat aacacactta aaagagtgga cttacctgca ttgggaaaac
    30841 ccactaaacc cacatcagcc atcagtttca aaaccagttc cagctccatc tcttctgttt
    30901 taacacctgg agttgcaatt cttggtgcct gatttgtggg agtggcaaaa ttataatttc
    30961 cttttcctcc ggagccacct gcggataaaa taaccttttc tccggcttgt gttatatcag
    31021 ccagtttata tttcttgttt tcttcaagca cataaatttc agtgcctaaa ggaagacgca
    31081 gaatacaatt cgcacctgaa gaaccggttt ttttagctcc ggcacctggt tttccgtttc
    31141 ctgccttgaa gaatttatga taacgatagt ccaaaagagt atttaaattg ctgtctccaa
    31201 cggcaataac atctcctccc cttcctccat ctcctccatc aggaccacct ttgggaacat
    31261 atttttcgcg tctgaaacta attgtgccat cacctccgtt tccggatttg atttttattc
    31321 gtgcgtagtc aataaacata tctgctgtta ctttgccacc acaaatttgc gcttaagcgt
    31381 ctgttttgcg ttttctactt tcataaaata tactcctgaa gataattgtt ttccggaggc
    31441 cattcctttc caattgatat ttaattcgcc gtttccggtt ggggactgat ttatttctgc
    31501 cactttttgt cctttgatat tataaatttc tatgcttaca ggacagttac acaagccctt
    31561 caaatttata tttaccggca aagaataaac cggattgggc atagtgcagg ataataaaga
    31621 gggaataagg taaccatctt gatttccaac aaaatgaaca acatcctgaa ctatatcaaa
    31681 accgttatca ctaaaccgcc aaataaggga atcaacttct atgacaggta actgtaaagt
    31741 gtaagttaca gggaaagaat aatccaaacc tgtaggcaaa aattgcaata ttgtgccatc
    31801 cggtaagata gcttcactta ttagaggaaa atccgcatcc gcatcccaat aatcaatcag
    31861 taaaattccc gttgagggat tgtaaccatt gaaagtgcaa acaggaagtg tattctggct
    31921 cacctgataa taattatcct gaaagataat aaaggcaggc gtgctataat caccaagccc
    31981 aatctgtggt tccaaactcg ctaaatctat atctactttc agcgtaagcc ctaacatcat
    32041 tatagcatct ggtaaagcac caaaatttgg atcggcagtt aaatcgctaa tattacaagc
    32101 aagatttaat gctcctccgg aaacctgact ttgaacattg cctaaacgcg taaaaacagg
    32161 ttgctgtgtg aaagaatcat aaccaacttt atacaacccg ctggaaatta aaccgggaat
    32221 attaaaacta tagaccatcg cataacctac actatctatt aacgaggtaa tattgcccaa
    32281 cacggcaaga tatacattgt aactggttag tgaattcatt atcggaaaac taccgctgca
    32341 gttcttcatt gtaaaatata ttttatcgga actaattgca gtgtaggact cccgtaaatc
    32401 cagattttga gaatatacag ttacactatc cccttctgca tctgttccaa tataagccag
    32461 attatctatt gaaggaggaa aagaattgct atcccaatag gcagaatgca tcattgctat
    32521 ggtttcaccc atttcttcaa tgctataatg aagtcggtaa cgcaattttt gattaaaaca
    32581 atagggtgcc aatgcttcct tttctcctgc agatatatca taaatagttg agttctgcca
    32641 gggcgcatca ttggcactat aataaaactc cggatttaat gtcccttcgc ttaagtcctc
    32701 ccaacgcaaa tgaatatagc cattttcatc tctggaacta tgacgaatgt tcccaaaagg
    32761 caattcatac tgagccatta gtaaagtagt tatacctact aaaataactg ttaaaatatg
    32821 tttcatcgct acctcacctt cctgaaactg gctaatacca tatatacttg taattcctgc
    32881 tgtcctctgt ccttactgaa aaagcgggaa gacattatcc ttctttgtgc aaatgcttaa
    32941 aggtattaaa atcactttac ctctattatt tataccattt ataatataat actggaaagc
    33001 ccagcttatg tcaagtattt tttgctgttc attttctaca ggaaaggttc ctgactaaag
    33061 atgatatctt caacaatagg attttataac agatttgccc taatcatctt attgagtgag
    33121 taatgaagag gtggtaatta tccaagcata gcttttaaaa tctgatttga gatcatatag
    33181 tgattgttta ctacttttgg ctaaaaggat atttccccag tcattcatag cagaatatta
    33241 caagtataga gtttcaatac aagaaacgaa acaagtagat attgattagt ttagaatgag
    33301 agctgtataa tagaatatta tttattagtt ctctggcttg gataacctga taataggaaa
    33361 ctatctggtt tcaaaaaata gctttgggac agaaaaattc taattcctaa aacatctaaa
    33421 caagaaccaa ttttaaacct aaatcacttc atcaaaacat aaattgttca agccgaattg
    33481 agttttctat tatatagata ttgctattcc gaaaagatca ataaaacata ggttttttat
    33541 tgttgtacca agtggaattt caaatcctaa aagtttaatc tccaaatgtt ttccctggca
    33601 atccatacaa gccgctggtt actccaaaaa cagatgtctt gctcaggatc ccctcaatta
    33661 tattttttgg ctattagatc caatagattc ggttatctgc ctcctatttc ttttgccacc
    33721 gcaacaacaa gttttaactc cagcactaca aactaagaac tttttgctta atggcatcat
    33781 aaatcctcac tgcagcagat gcattataat tgtcaaaaag ttcttgacaa tataaatagg
    33841 atatttatta gtgtctttat gaaatgtgaa ggtttctgcc gaaattatgg aaaagcgata
    33901 aaggactgaa gagaaaagga gtttatgaag atgaaaaata tcagcttcaa gttaaattcc
    33961 tgcaagagga atggtttttt tgccaggcaa gagaagaacg atagtttgtt cttgatgaag
    34021 aaatatagat atatgtggtt aggaagtgcg gtaaaaagtt tattccttat ttacctacga
    34081 ttatcaaaag atgttatttc ggttccgctc tttttgttca gaaaaaggga acattatcac
    34141 tggggcggtg tatcaataaa gcaatcagga aaacaatttt ttactactac ctactaatta
    34201 aggaggaaag atgaaaaaat cacttgtcgt tctgcttatt gttttggcga tgagcagttt
    34261 tctctttgcc gcggagtata ccattgtaga tggtagctcc aatagttatc atattcccgt
    34321 taacggaaat tcccattatg gatggagccg atttatattt acggctactg aaatgaatac
    34381 tgctggaata accggttcat ttactattta ttctattgct ttcaaaatta acaatactgt
    34441 ctccgattat actatggata atcagaaggt ctactttggt tacaattata acagtactct
    34501 ttctggttca acaagttatc ccaatcccgg tggcggtagt tcatttacca aggtctatga
    34561 tggcagtata acttttaatg gacccggctg gatggaaatt attttaacca cgcctttcaa
    34621 ttttacttgg gacggttatg cgggcttaga tattgtttgg gaaaactggg atggcagtag
    34681 aattaccagc ggttatccca aattttatta tgcttccaaa agcaatatgg cagtttacaa
    34741 agaatccgga accgggactt cttttccagc cagtactgga acaacttcca ataatcatcc
    34801 cagtttcaaa attattactc cccctacaga acttcccaat cctgcaataa atcctgatcc
    34861 tgtaaatggt gcatcaggag tagtaattga taaagaactg aaatgggcaa gcggaggtgg
    34921 ttcacccata aattacatag tcaatttggg aaccgataat cctccaacca atattgctta
    34981 taatgaagtt gttactgcaa ccaaatacac tcctgcaaac agatttgatt atgggactat
    35041 ctattactgg caagtgatac ctcataatat tattggtgat gcacaggatt gtccaatttg
    35101 gagttttact acaatggatg atccttccat tgccactttt ccttatcagg agagttttga
    35161 tggcactttt cctcccaata gcgactggtt aatgagaaca ggagttttgc aagacccaat
    35221 cactttaagt cctacaagta tatgggaaca ggatgactgg ctgaatattc ccggaaccga
    35281 taaggcagcc agaatcaata tctgggggcc ccttaatgcc tggttgatta cccccttatt
    35341 taatattccg gatgatagct attatctttc ttttgatttg gcagtgctaa aaagtgatca
    35401 aacaccaaca ggaacacctc cagtttatgc tcctgatgat agaattgccg ttttaattgg
    35461 agatggatat aactggtcta cagccaatat agtgcgtgaa tggaataata cgggttctcc
    35521 ttatgtttac aatgatatca gaatcagtgg agagaaagta gttattccct taaccgggca
    35581 tagtggtcat tgtcgttttg ccttttatgc aggttcaact gaatacaatg ccgataatga
    35641 tgttatgttt aacaatatgg aagttgctgc tttcagtaat cttcccttag ctgctcaaaa
    35701 tcccattcct gaaaataatg ctcaagatgt acctcttaat gctaaactgt tttgggatgc
    35761 gggaggcaat gcacctgttt cctataactt atatttggga caaactcttc ccggaacacc
    35821 tgtagaatgt ttatcgccac attacactcc tgcagcttta gcttatggaa caacatattc
    35881 ctggaaggtt gttcctgtta atccaatagg ggaagcaatt gattgtcctg tttggaattt
    35941 tactaccact ggaacagcca cggttggtgc cggtacagtt accatcaatg atgttccgat
    36001 aaatcccagc gtagaaattt ccggcttgga aggtgaaaca gtaataagtg ccagtgccag
    36061 ttttgctccg gaaggtattg gtttacctaa tgctggttta gtaattcagt taagcagttc
    36121 gggagttaat cttacaggaa aacatataac gattaatcat tcgcttggct ttatcccttc
    36181 ccagattgct tatcgtattt tacccagtga aacatatcat attatttcca atccgggaac
    36241 ctggactgct gaaacggtta gctttatttt ggaagctaaa gcggatggtg atgtggatat
    36301 tgtttttccg ttggatgcag aatccacttt gcctgtggag ctatcatatt ttaatgccat
    36361 atacacagcg gaaggttatg ttaccataag ctgggtaagc caatcggaga caaaccatag
    36421 cggttataac attttgcgta gcgaagatag aatatttgcc aatgctattc ggataaatcc
    36481 agttttgata gataaaggaa ccgaacttgg aactcagatt aaatataaat ttattgattc
    36541 tgaatttgcg cctaatacgg tttattatta ctggctggaa agtatttctt tgaatggtga
    36601 aacgcaatat tttggccctg tttttgttat gacgggagaa aatggttctg aaccagaaac
    36661 tcctgaatta ccctttataa caaaattgta taatgcctat cctaatcctt ttaatcccca
    36721 tacggttata agttacagta tatctaaacc ggcaactgta actttggaaa tatataattc
    36781 taaaggacag aaaatccgtt cgttcataaa caaccataat tcagcgggaa tttacagtat
    36841 gaattgggat ggcaaagatg atacaggtaa aacagttagt agtggtgtat atatctatcg
    36901 tatgaaaacc gataatttcc aacaaagcaa aaaaatgcta ctgacaaaat aattcgctga
    36961 aagcggaagt tagtgtaaga atcccgggtg aaaatgcccg ggattttttt aactattgag
    37021 aacaccgaaa acttttaaac tgcagacaga cctcttgact tcaagaactc tcgccttcgt
    37081 gctctccagt cctcaatggc ttaaaaaata agtaagcaag caaatagaga actgcccaac
    37141 caataaggaa accgagaatt ctgcctggtt gaaaaggact aaaaaactgg aacaggacaa
    37201 actgtttgct ttttgcctga taattttgta taaaaggcgt gaaaacagaa tccggggtag
    37261 cggaatttac ttcttccaag agcgcaacaa gttctaggtg ttcactgcgt ttgtttttat
    37321 agtgatattt atcatccagt aagtttttct ttgccagttc gggcagtttt gcctggagag
    37381 caataacgcc ttgttgaaaa agttcccagg ataagctgtc gtccaccaat ttatagtatt
    37441 cggataaatt tacgagtgtt ttcaacattc cggaaaggga atagggttcg gtaggaaatt
    37501 cctgaaacca aatgccacct tttttttcat aaaaactgaa attgcctttt tccggttgca
    37561 gggtatagag cattttcctg gctttaataa atgtttcggg gctacgcagg ttgcctgctc
    37621 tttctgttaa agcaagcatt gttgcactat gactggcgga actgaaccag ggttttttta
    37681 aatgtgcttt tgggtaatca taattatggg gaatattacc attttcatcc agatgctctt
    37741 ccagccattt actatacagc aaaaaggcat ttttccgcgg ttcctcaatg cgggttttat
    37801 aaaagcggga ggactcataa gcaatgaatt caggatcata aacctttccg gcagatggat
    37861 aataacgcat aggaatgcca tttttatcca ttgtaggcat ttccacaaaa gaactgccaa
    37921 aaatacttaa acaggtctta ctccaaaaat cggcaataag ggtctctgct atttgccaaa
    37981 ttgctatggc aaaaataaga ccgatgagca aaggtatcag gtgatgtttc aatttattca
    38041 tatttacctt cttttaagtt aagaatcatt tacacttcaa gtgaggagtc attggcgtct
    38101 ttataacttt tgtccttgtt gataaccttt ggacgcaaag gactcatcac gacgataaca
    38161 aaacctctca taggttacac tttctaacac ggataaaata ggtaagggac taacagtgtc
    38221 ttccggctac acaaaacttg cggaggaacg gcatcctact tctacacaga gaaaagcaaa
    38281 gagagaaaat aaaggattgc ttcaaataca acaatacaag caccagtagg taaattcagc
    38341 agaaaactaa aataaaatcc tataaaagcg gaaaaaacag ctaccagaac agagataact
    38401 attgctttgg aaagccgatg cacaagatta aaagcgatta ctgcgggcaa aataagttga
    38461 gccgttacca gaataattcc tgcgcttttc acattgataa caatattagc agccaaaagc
    38521 agcataaaaa tcaggttcac caggtctgtt ttaatccgat agaccttagc catcacagga
    38581 ttataggtta aataaaagag ttccttataa aaaaagcaga caaaggcaat attcaaaacg
    38641 gataaaacag ccagagcaat tatttcgttt ctggtaatca gcagaacatt gccaaaaagg
    38701 tagctggcaa ggtcaaaact gtaatttttt gaaagagaaa ttaggattat acccagtgcc
    38761 atacttaagg ataaaaatat ggttattgta ttggattcct gaatgttatg ctttttggcg
    38821 agcaatgtta ttcccaaagc aatcagcatc acaaaaatca gggtagtaac agttaaattc
    38881 aatcctgcca atatagccag ggcaattccg gcaaaagcaa tatgggaaag tgcttcaccc
    38941 aaataggata tccgccttaa ggtgacgaaa ggagcaaaaa cagctaaaga aattcccgaa
    39001 agcaaagcac ccaaaaaagc gtaaaccaga aacgaaaagc taaacatttt tgcctaataa
    39061 tccttttcaa taatccgcaa actatcacca aaggttttat gtatcacatc tgcatctata
    39121 agttccatct gattatgaca atgcaaacgg cgattcaagc aaaccagaaa gctgcaatat
    39181 ttagagagag tagttaaatc gtgagaaata gttataatcg ttttgccttt gctattgagt
    39241 tcttttagca gggcaaaaaa actttctacg gaaggacggt ctaaacttgc ctccggttca
    39301 tcaaggataa gatatttgct ttccgatacc agggcacgcg ctaaaaggac tctttgaaat
    39361 tcacctccgg aaagttttcc aatcagttct tttgccagat gagcaactcc tgtttgttcc
    39421 aatgcctcca ttgctttttg tttatggttt ttattaaaat gcgttcccaa aggaagttga
    39481 cctgccacac ccattaaaac aatatccaaa gcggttgcag gaaaggcatt atcaaattcc
    39541 tcatattggg gtagatagcc aatggtatta tttttcagcc agttatagtg ttccacacca
    39601 tctataaaaa tactgccttc tttcaattcc aataaaccga gaatcagttt taccaaagtt
    39661 gatttacctg ctccgttagg acctataata gcggcaaatt caccttcggc aagttccaga
    39721 aaaatatctt ccagaattat cttgccgttt attttgtatt ggaggtcttt aatttgaatc
    39781 attagtattg ctaaaatccc gctttcatct tttgccagtt gctatctatc aattcagcaa
    39841 tggtttgtgc tttgccgtca ctacctaaag gatcaagggt aagcagtttc aagttaaatt
    39901 ctttagagag tgtttcacca gcttttctat ccatttgcgg ctccagaaaa atagcttgga
    39961 cattatgttc ttttatgatg ttgcctagga gagcaagttc tttgggagta ggttctttac
    40021 caggactttc ctgcacaaag cccaattcct caatatcaaa ttcctggagg aaatagtgga
    40081 aagcattatg ataggtaata attgccgggt ttttgtattg agctctttcc gttttaattt
    40141 tatcaattag attttttagt tcctgttgca tttgttgagc attgtgtttg aagacaaaag
    40201 aagaattggg aaagcgagtg ctaagttctt tttccagaaa accaatgagg cgaatcatta
    40261 acgagggaga ggtccataaa tgggggtttt gagcgtgttg atgttggtgt tcaactggtt
    40321 caggaacttc ttcttccgca agtaatttcc tttccggcag aggaataata tctttcagca
    40381 aagtagcagc ttccacataa acatcttttc tttccgcaaa attcttttcc agatttgcct
    40441 ccaatcctaa accattggca agaatgaaag aagcggaact aaagtttttt aaatcggcag
    40501 gaccgggtga ccaggtatga ggtgaagcat ttgaaggaat aatacattta acctcaaatt
    40561 catttcccgc aagttgtttc agaataagct cgtagggatg aattgaagca agtagcaaag
    40621 gactttgctt ttcttcctgt ttttggcagg aaaaagcgaa gagcaaagtg ataagcacgc
    40681 tccatttaag atacttcatt tttacctcct aagcttttat acaccgagaa cacctaattt
    40741 accgaaagca ccgagtttat tttataatta ttagcaatgc taattatcat tttcaattct
    40801 caattcagca tataattttt ccggctaact ataaaccatt ttcccaattc catctttcca
    40861 tctcatcatc tcgctatttt tagggcacct cactttattt tagattttac agttcacaat
    40921 ttcctatatc gcatcatcca cccatcgtaa aggatgggtt gatgatgggt tgatgatagg
    40981 tagcaagcaa ggaagaaatc ctattccata tattttaaaa gagttgttag caaacggaca
    41041 cctgctcccg ttgcaccgta gttataaatt ccgtattctt tatctttgaa ggcggttccg
    41101 gcaatgtcaa tatgcagcca gggcttattc tgcacaaatt cttgcaggaa aaggcccgca
    41161 gtaatagtgc cggcagaggg acctccggaa tttttcaaat cggctatttc ggatttgagt
    41221 tgttctttat attcttcaaa agcgggaagt tgccagatat tttcaccggt aaagtgagct
    41281 gcatttttca gctgttccag ccagggttcg ttattggtaa tcacggcaga aatttgtgtt
    41341 cccaaagcgg taactgctgc acccgttaaa gttgcgatat caataatgcg gtcgcaatgt
    41401 tctttttcca gagcataatt gatagcatcg caaagagtta agcgaccttc agcatcggta
    41461 ttgactactt caatagtttt tccgctcatt gaggtaatta catctccggt atgataagca
    41521 tcccctgaaa tcatattttc gcaggcagcg atgatgccta ccacattaac tttcaatttc
    41581 atagtggcaa ttgcacacat agttccgata acggcagctg cacctcccat atcatctttc
    41641 atatttgaca ttccttgtgc acttttaata cagtaaccgc cgctgtcaaa ggtgatacct
    41701 tttccgatta aagcaatggt ttcctgatgg tgttcaggat ttcctaaatg gcgcataata
    41761 ataagttttg gctcgtgagt tgagccgcgt cccacagcta aaaaggcatc cattttgatg
    41821 cgtttaagtt tatccaggga atgcacatca atagaaaaac cgtattgcaa agctgcctgt
    41881 ttggctgttt ccgcaaggtt ttcgggagta ataacattgg caggttcatt taccaaattc
    41941 ctggcaaaat tggttgtttc ggcataaatg cgtccttcta ctatacctct gtttagatgg
    42001 cgggtatttt tagagaggag catataatgc aatgcctcaa tttcgctttc gtttttagcc
    42061 gagcttgagt atttattaaa ttgataggta accagtaaag ccgtttcgga aagcaaatga
    42121 ccaaaagcca catcggttat agggcaatca aacccttgaa aaagatagat ttgtttggct
    42181 cttaatttgg cagcgaaacg aattgcggaa gccaaaagat tgcggacttt attagtattg
    42241 aggtcgtttt tattgccggc acccaataaa ataatatttt gtcgttggat tccattcacg
    42301 atggcaaagt tctttaaatc cccgtaagcg aacttaaaat cctcactttt acaatagacc
    42361 tgagccgttt tagcgatgtt ttgcggtagc aattctacct tgtccaatgc cccgttttct
    42421 tcctggaaaa tgattataca atccatatct tccgggactt tacggatgat gtcgatcttc
    42481 attctgaact ccttattgtt tttttatagt atattaaaaa tcttcggata aacttaagta
    42541 atacatcggt ttggaaattt tagagaggtc agtaagccag gcaatatcaa attttaacac
    42601 aaaataacct aaattgagac gaggtccgaa accgtaaccc aattttatat cattcagttt
    42661 accgtctatc actcccctaa aatcagaatt tttatcccaa gcagctccca tatcggcaaa
    42721 aatgcttccg cgaatattgc gtagctgtaa aggtacggga aaagccaaat agagataatc
    42781 aaagaaagga aaacgcagtt cagcagaacc caaaatcatt ctttctccgt ctatatcttc
    42841 atttaatgcc cgcacaccat acaaaccatc aagattaaaa cgctgaggtg attcacctgt
    42901 actgattcct gcattcaaac gaaaagccag agaatagcgt ttttcaaaaa agttgtaaca
    42961 gcgcagatca agatagttgg taaaatagtt cagctcattt ttagcaaagc ttttccgaat
    43021 gagataataa taccgccaac caaccatagg tcctgtagag ccgaaaagag cattatcaaa
    43081 aacaaaagcc aagccaggtg caataaccgt atcggtctct ttggagatat ccatttgcca
    43141 gttttctgta ggaacatcct ccggtgccag ataatcccat tcctgtttca cataatacag
    43201 ctggctgtca aattccaaac gcgtaaaccg actgaaggga taacggtaaa taaaaagcat
    43261 tcccagttcg cgttctcgca agcgaaaata atcatcctga ccgatcagat aacgcctata
    43321 aagcgtttca tcatagaaat tatagacccc tattccgtaa tcggcacgat gctttagatt
    43381 cataaaagaa aggaaaagat tgctgtcttc aatttttcct gaaatcccca aactgatacc
    43441 tattccataa ttgcccatta aatcacttaa agtgagatca atagctccca cggtgcccgt
    43501 gtaagatgaa tacgccaatc cgccccagat gctgtctaaa gcgaattttg ttttatattt
    43561 ttctattttg ggcggaacat taatactatc cggtttatca tcccaggaaa aatcctgaaa
    43621 atgtttcaag gaatcgcttt tgtccgattc attattggaa atatgaggac gacggggatt
    43681 ttgttgtggg gattgtggtt ttgaaagatg ttgtgcttta gttctttttc cgtaataatc
    43741 aagacgggta aggtctatgg tctgtaaaag gtcatcagca cgcacaaaac tttgcggttg
    43801 gctgctttct tcataaatta gatttttcag gggattatcg tcaaaataga tatcccaggc
    43861 accgtcaaag tagttgctta taactagata actatcatca aagctgatat cgccattcat
    43921 aataccggca atgcatttag tgagttcagc gcgctgtccg gtttcccaat ccagaatttc
    43981 ataattggca aggttatatt tataggaaag aaagacgagc ttggaaccgt cttctgccca
    44041 aattggactg aaacaatcgt aattttcaaa ggttctttca actatttcgc cggtctcaat
    44101 attgaaagtg aaaatatcgc gcgtaaggtc tgaaaacaaa ccataacgga tttcctgctt
    44161 ttcacactta tgttcactat cgtaagcaat gtatttgccg tcgggtgaaa aacggggatg
    44221 agcatcgtca taactgtcat cagttagctg agttaatttt cctgtttcaa tttcataaag
    44281 atagaggtcg gattgcatat ttttttgacc tgccaaaacc agaaacaagc catcgggaga
    44341 aacatccgct tcataaattg cctgcaattc cggaatgctg atcgtctgca ctattttttt
    44401 agtgtttaca ttcaggattt gtattttatc accggtagct gtcttggcag aaaaagcaat
    44461 tctttgatta tcgggaaacc aggaaagagc cgaacgcaaa tagtaaaatt cttctgcctt
    44521 gccacttttc tcacccttaa aaatcaattg cggttgagct aatccctgag ttccagccag
    44581 ccaaatactg tatcttccac cggcattaga ataatataca tagcgtgaac cattaggaga
    44641 aaaacgaggt gccaaattga aataggaacc atctttttta tgaaatgtcc gctgttcaaa
    44701 attctccatc ggaatatcgt gactatttat cagaggatag taatctctct ttaactgata
    44761 atgccagcgg gattccaggt cttcaaaatc cattccgaaa acctttttcg tagcttcatc
    44821 aagattgaat gaggaatgaa tggcaaaaaa atactcggga actttttctc tgccccaggt
    44881 ttcagctata aaagttaaaa aactctcgcc taaacgatat gccaagtagc catcggcact
    44941 gttcaatcta ccgatggtat tatttatcac catatccaac aaatacatat tattatagtc
    45001 atcttcgcca ccaacggaaa gatattccgg aagtccttcc gaaaaccaaa aaggaaaaga
    45061 agtgggacgg agcatagaat tgggatttac aaaacgatta tccattgcat ttatataagc
    45121 atgagtaagt tcatgtgcca aaagttcttc cagattggca taactgcctt caaaaggaac
    45181 cacaacacgg ttatgtaaac tttccgtaaa accgcctact ccttcggtta aaagataata
    45241 agttatattg gtggataaaa attcttgctt agtactgtaa aaaataatag gaatccgggc
    45301 taaaatcgga actttgaatt ccgctttaat ataataatat atatcttcag ccattaaagc
    45361 cgcaatgcgg ccaaattgat cttcccctgc gggaaaatag agatcaaaat gcattgtttg
    45421 aatggtagag aaatcctgag gagcaaaatt aactttattt tgaccgaaac tgtaagcaaa
    45481 aacagaactg cataagacaa agaggattat tagacaatat agctttttat tcattagctt
    45541 tcctaaaata cgctgaattt aatgtatttt ttggggtttt ccttaatatc tttaatgagg
    45601 gcattgagat tatcggttgc attggtgatt ttttcatata actgcttatc atttagtaat
    45661 aaaccagcag taccttcgtt ttcggtaagg gctttggcag aacgattcaa attggcagaa
    45721 agaattttta acgaatccaa tgtagcagaa agtttctcta tagttatgtt accttccacc
    45781 aaagtatttt ttaagggttc tttattttcc gctataactt cctgcagaga agaggttaaa
    45841 tcctcaattt tattcactgt aataacgagt tcctgtttta tttctttagc tacattgccc
    45901 atttcagtta ctgtgccatc tgcattttgc actaacattc gggaactttg cagtaagcca
    45961 tcgggagaat taagtaattt aaccgtttca ttcaattcat ccaagcccgc agatgcttta
    46021 ctaatcaatt ccatcatacc ttctggggaa gttccctgtt gcaatttttt tatatttagg
    46081 ggattttttc catctcccgg aataatgttt aatgcctttc cacccatcaa agaactgtcc
    46141 gaaaccagaa accgtgttcc ttccgttaaa attatatcac ttctgatatt ggcagttgta
    46201 attaccttat ctttgctcgc agttatgctt tttactctac caacttccat tccgcgatac
    46261 ataactttat cgccaacttc caaacccatc acattcggaa aggcaatttt cagttcataa
    46321 tgggaacgga ctgctaaacg accgctaagc caaaaataac taaaaaccag aatcagacag
    46381 ataacaattg tccagatacc tgtttttatt tgcacgctac gcaggttagg atagaatttc
    46441 ttcattacct tttaggtctt cagctgtttc ttttcagcag cggggaagag gacattattc
    46501 agaattaaac gatagccggg tgaattttta tgcagttcta atatggtttc cggatctcca
    46561 actttatgct gataatcctc tggatcatga cctccgtaaa aagtgaaagt ccctttgcca
    46621 atattgccat gaatgtattt aacttcattc tgaccgggaa cttccgctaa aataatcaca
    46681 cttttcttta ccttatcctt gaaaaaagaa gtggtctgac ccagaaaacc gtttataaca
    46741 ttagtatgac attgcgtaag cattgtggga acaggatcat acttggcgct gaaatcaaaa
    46801 agctgaaaat aatccgcttc gggacctctt aaactggaat aatcgctggc atctatatcg
    46861 gaaaattcat attcataagg attgggacgc aaagtaaaat tttcaaaggc aaaagtgcgg
    46921 gaaaaatcca gtttactttg atactgaggg tctatgccat caccgtcaat agcggcatct
    46981 acaatatcta catctttggc tgctaaagca atatcataag tatcggtagc agcacacata
    47041 gcaaacataa aaccaccgtt tattacatag ttgcgtatct tttccgctac tgcatgcttc
    47101 aattgccaaa ccttggcaaa attatgttta gcagccattg cttcatttac acgcacatca
    47161 ttctgatacc agctcgtatt tctgtaagaa ccataaaact tgccatactg acctgtaaaa
    47221 tcctcatggt gtaaatgcag ccaatcatat tccggaagct tgcctgctaa aacttcatca
    47281 tcccaaacac ggtcataatc aatttccgca taatccaaag ccatagttac tgcatcatcc
    47341 cagggaagag cattaggagg tatataaacc gctattttag gttctttttc cagggcaatg
    47401 ctttccatat tatttgcctg aatttccatt aaaattgccg ctacatcaga agaagaaaca
    47461 ttttcataac gcacaccccg aatgttacaa agagcagtta aactttcgga atcgggaaaa
    47521 agaaaacttc ctccccgata gtttaaaagc catctgccaa caagatgttc tttcagaccc
    47581 gcaaaggcaa tgccataggc ctttagatgg ttggtctgcg tgttatccat agggattaaa
    47641 atatcggcag ataaaggtat tatagtaaaa ctgctaaaca ctattaagca tagcagaata
    47701 actgatatcc tttttcctcc agcacacacc aacgcttttc taaacaagga tttatcctcc
    47761 ttaatttaat tcctgcttat ataaagctat ttttatgcca taaaacgcat aaatgtcttc
    47821 tgctgtcaag ataaatctca caaacaacga aaacaaagag gacaccgaag aaaaagagta
    47881 ttttttacac ggaaaacact gaatacaccg agagcacaga aaaaactata caactaataa
    47941 acttccaata tagctccagc cctctctacc cctttcactg aaacacataa atcagccacg
    48001 gaatataaaa cagagcaata aaaatggata tagaaacaat agtagccgca aactctctat
    48061 ccaaattttc caaattagca aaaataagag tattaaaacc aataggcaaa gcagcacaaa
    48121 cagttactac agttctggtt attcccttaa acccaaaaag agcagataga acaataccta
    48181 tgcccaaacc aatgcccatt ctgataaaaa tggcaattgc cgcttttccg atattacagc
    48241 gtttgggatt aaaataaagt cctaaggcaa tcatcattaa agggaccatc ggttgcccca
    48301 taaaattgag aaaattcaaa gctaaagctg gcatcgggac tttagttaat ttccaggaaa
    48361 aggcaattaa catagcccaa agaggaggta aacgcaaaaa ctttttccac tcaatttcag
    48421 ctgtcttact attatcacca tactttatgg cattataata ggtaaaggta aaaatcataa
    48481 aggtattgcc aatatcaaaa agtgatgctc tggcaagtcc ttcattgcca aaagcagcaa
    48541 agaaaaaagg caaggcataa gcagtattca taatcattgt tcccaccagg aaactgccga
    48601 aagtggtttt ttccattttc aagtgttttc ctatagtgcg ggagataata tatatcagaa
    48661 agacaataat aatagctgta aatggaatta aagccatatc cgcttttagc tgaatattgg
    48721 aaattgcaat aatggttaaa gaaggtaaac atacattaag cacaaccctc aaaagaacag
    48781 gcgcatcatc tttagacaga agctttagaa gtttgaaggc aatgcctaca aaaaaagcca
    48841 gaataagagg aattattttt tcaataaagg gattcattgc tcaggaggaa gataaaaagt
    48901 gcgaaatagt aggttaaatt gttcttccag gtcttgatta tgtagcttat ctctttcggt
    48961 aatttctttg cttatttgcc aggaagggtc ttttgccagt gtttcttgca attgccattt
    49021 atgcctgaat tctcttccct caagtttaac taatgcctcc cattgttgtt ccgtgaaacg
    49081 aattgccaaa cctttatcct gcagaagctt ggtatttact ttcaaaggat aaacctcagt
    49141 gatacggata attttcagta tttcagaacg cgaaaaatat ttttctgcct ttagttcttt
    49201 ataaaccacc atctcgcggt aaaatttacg accattcaca gaagacagat gattaacatc
    49261 caagccctct ttttctatct gacggatagt tgtgtgattg aaagtatata ttgttttttg
    49321 gtaaacgttc tgttcaaaca cattctgcac aataaaaaga gctataaaag caaagaaaaa
    49381 agcaataact ggtgcagcta tccaagccaa agataccttg cctaatatat tgtaacggat
    49441 atttttaccc ccttttacca aaccaattcc aacaaccgct cccacgataa tttgggtgga
    49501 agatacagga actaaaggaa tgggaggtaa acctatactt aataataagt tttgcaaacc
    49561 acgcgaggca aaaataaaaa ggacaattgc ttctgatgct acagctataa gtgctgttac
    49621 cggtgaaaga tgaaaaatat cttttcctac agttcgcatc actttatgcg aataggtgta
    49681 aattccgaca attatagaaa ttgcacccaa aatatagagt tgctggacac ccgaaagagt
    49741 aaaactgccc aaattaatat ctttgaaagg agttaccgga acaaaaactc caaccacatt
    49801 ggctatgtta ttagcaccca aagaaaaagc accaaaggca ccgattataa ttaaccctaa
    49861 acgaacataa acatcttgct ctaaaagatg aatttttcct ctattgataa cgggtctgat
    49921 taaataaaaa agcaaagcgg caataattcc ggaaagcaca aaagcaatta cccagctgga
    49981 aacaatggta agtaaattac gaaagtcagt tagccgtccc gaaaaaaagt tccaaccaat
    50041 aattgcacct acaatggttt gagaagtgga aaccggaatt cccgttcgga caatagcagt
    50101 aatagttaaa gcagctgcca gggcaactgt aaaagcacct cctaaagcat caacacttcc
    50161 taaacgacct aaagttccgg aaggaccgct accttcaaaa acagctccta aaaagataaa
    50221 aatggacgca attaaagccg ctgttttgaa tttcagcatt ctggtttcta cagctgcacc
    50281 aaaaatattg ccggtatcat tggctcctaa agaccaacct aaaaaaagac cgctgagcag
    50341 gtaaataaaa atcatttata gttgacgctt gatagcataa atagataacc gatcacaaac
    50401 aacctgcgca taatccgaaa ttttttcaat atgtatggca aaaaaacgca gctgactttt
    50461 ttgggaaagt tcaatatcca aattgaatat ttcacgacgc aggcgttcac ctatatgatc
    50521 cgcttcgcgt tcaaagaaat agaccttgtg aatgtaattg tttaccgcag ctaaatccct
    50581 aaaaaatgaa cgcactgcaa cagagagctg ttcaacggca ttgcttgatg ccttagttgt
    50641 ttgcatccac aaatcattta agcactctgg aatttttggc atctcaatgg aaaattgaac
    50701 cagagagtcc tttatattat caataacctc atctgtattt tctaagatag caagaacatc
    50761 acctctgttt tccggaatta gagttcgttc ataaagaaag cgttcaatgc taacccgtaa
    50821 atcatctgcc cgatgttcat tttccctgat ttgagccaaa cgggtttcaa attgttcaat
    50881 ccggttattg aggtaatctt ccattgccag ttcaaaagtg gtagccgaat cacttaaaat
    50941 atccaaaaag gcatctattt gggtttcaat aaagcgggtt gtttttaaaa tcagagccat
    51001 tttctctcct tgagagttat aattttattc cttttattat tttaccagaa aaagagatac
    51061 tactttctgt caagcgaaaa gggaattcag gttaacggat gaacaggtta acaaatgaaa
    51121 ccccaagata aattaagggc aacctgaaat atctctttta gattgaaaaa gccctttgcc
    51181 attctcgcgg aagcaggaat tcataaaagt tttaacaaaa taactacatt ttcaatttgg
    51241 aaactatgta atttctattg cacatacttt gtaacgggta atatggataa ataattggat
    51301 gctcaataaa aaaattatgt tagaaaaaat agaggttact tttatgagga cggggtatga
    51361 ttttaaggat aaaatgaaat atcccttgac aatagaagtt acttgagata gcagaactat
    51421 ggaagttata tgaggtaatt ttatatgatt caagctatta gctataaacc cctgaattta
    51481 gagggagaaa aatataaaaa ttcttaatta attgagcttt tgaatactgt attgtttagg
    51541 aaaatatagg attatttggt tagtaaagtt aaaaggacat taccgataat ttacaaccat
    51601 ttcaatttta tagcgttatt caaaagtaca taatatatat ttcaaggtct ttaaaaaata
    51661 aataaattca agaggaatta tgaaagcgga cacaacaaac aacactttta gtgaaattat
    51721 tctttatcct gtagaggaag gaaaatatga aatagctcta aataaagata gtgaatctat
    51781 ttggttgaat ctcaatcaaa tagctgaact ttatcaaaca tctaaagcta atatcagtat
    51841 gcatatacat aacatcttaa aagatggtga attaaccagg aaagcaactg ttaagaatta
    51901 cttaacagtt caaattgaaa agggacgaca agttcataga aatataaggt attacaactt
    51961 agatatgatt ctggctatag gttaccgggt ccattcagag agaggaactc aatttcggat
    52021 ttgggctact caacatttaa gtgaattttt gcataaaggt tttatacttg atgatgaaag
    52081 attgaaaggt aacgatcgtc ttacggatta ctttgatgaa ttgttagctc gcatcagaga
    52141 tattagagct agtgaaaaaa gagcatattt acgggtaagg gagatttttg ctctggcaat
    52201 tgattataaa cctaatagta aagctgctaa taagttcttt gctactatgc aaaataaaat
    52261 gcattatgct gcaaccggta tgacagctgc ggaaattatt gccacaagag ctaatgctca
    52321 agttaccaat atgggactta ctaattggaa aggaaatata gtaagaaagg aagatttgtc
    52381 tattgcgaaa aactatctta cttcagatga aattgatatt ttgaatcgta ttgtaaatat
    52441 gtttctggaa caggcagagt ttagaacttt aagaaaacaa caaatttata ctaaggactg
    52501 ggagaagttt ttagacaaat ttctgatgga taatgaactt ccaattttaa atggatcggg
    52561 caaaataact cataatgaag ctaaagaatt agctgagaat gcttataatg aatttgaatt
    52621 aagacgcaga aaagctattg aagatgaggc tgaagcaaaa tatttagcag accttacaaa
    52681 agcaactaaa ttagtttctg caagaaggaa aaaaagataa ctatgggtta acaggtgaaa
    52741 agtttaacag atgaaccccc aagataaatt aatggcgacc tgtaatatgt ttgcctgttg
    52801 caaatacaat tatatttttt acttacctgg attcctgctt tcgcaggaag gataaggtaa
    52861 tttttatgct aaaaatgata caacttcttc tcgtagtata aattgacctc tctacctcaa
    52921 gaccttatcg caaaaactgt ttcaacgcat taacaaatgc ttcaacatct tccttgcagc
    52981 taatcgttac ccttaaacaa ttttgcaaac ggggatgaga acctacatcg cgaacagcaa
    53041 tatcgtttgc taaaagatga ttaaagaatt caggagtccg ttctgcaaga gaaaaagtaa
    53101 ggaaattggt agcagaagga taaaccttta tctcttctat ggtctgcaaa gttgcaaaaa
    53161 gctcttcccg caattgaatt gtttgttgta cctgagcttt aaagatatcc tgattttcca
    53221 gaatagtgag agcaaatgcc tggttcagaa tagaagtatg aaaggtagta agcactttgg
    53281 aaagttcata aatgttttcc gctgaagaaa ttgcatagcc aaaacgcaat ccagcagagg
    53341 aaaatgcttt ggaaaaagaa cggactaaaa tcaaattggg atactgcaat aatttgtctg
    53401 ccagtgtcct tccggaaaat tcaaaatagg tctcatcaac cagcacaagt ttatcaggca
    53461 aagatgtaat tatttgtaat agttggcaat cgggaaacaa attgcccgta ggattattgg
    53521 gattgcataa tatggctaac ttacaatccg ggtgactggt aagttccaaa tacttttccg
    53581 gggagaaact gaaatcctca tccaaatcca aaaatagcat tttcagtcct accatagcgc
    53641 taaaggtttt ataatcaaaa taggaaggtg ccaaggaaat agcaaaggat tgatcatttt
    53701 cccgcactcc tagaaagata tgatagagaa tatcatcagc tccatttccc cacaggacat
    53761 tttctttagt taaggaattg ccaatgtaag aaattaggga taaatgcagt ttttccgtta
    53821 caggagagag ataacggtta agttcaatct cttccatttt ggctaaaaat tgctgtttaa
    53881 ttgcctgata gggatttagg caactttcgt tcaggcacat cattcttttt ttaacgggaa
    53941 cacttatttt agagggcttt ttatctttca agtcactgcg aaaatagggc ataattcttt
    54001 ctccttctat caatattata agttgaaatc ggggaaaagc aaaagtgtca ttcttatccg
    54061 gtttccacat tgaaagtgca gttattattg aaaaaatatc cttgattcct gcttgtgcag
    54121 gaatgacaag agcatttcat tccgataatg attttatttt aaaaaaaagc cacgggagga
    54181 gtacccgtgg ctttgtctgt gtgttgctga ggagggatta ttaggggctt atttttcttg
    54241 ctctattgtt tgggaggtga ttttttttca ggcataccga aagaaccgca ttctccgcat
    54301 tcatcacaat tttctctgct ctgaggtctg aagtccttag catctttaca gggtgttggt
    54361 ttatgcatag caccctgaaa atgtggcata ttttttttca gggttgcctt ctgttcgggg
    54421 gtaagttctt tcattctggc agccaaaaaa tccactttag catcggctaa gacattgcgt
    54481 ttagtggcta tttgtttgtt taaatccttt gcctgcttgt agttttcatc tttcaaagct
    54541 ttctgcaggt caatttttaa atttttaatt tcggcattta gggtgttttt atttttctca
    54601 aaacttattt tcagttcttc aaattttttc ttttgggcat cggtaagttt caattcctca
    54661 aggcattttt gcatcggcat ttcgggtttc ggggatttaa tcccttccga ttgaacctta
    54721 ggattagagc gatctttagg ggcatccgtt tgggcaagta atgcaccgca aacgaatatt
    54781 agggcaaggg ttaggaatat aatttttttc atctgtttct cctgttttta ggtatattat
    54841 ctctgtgtaa gcgttttttg gcaaattcag cacggcgttg gaaatgttcc tgtgcttctt
    54901 ctgccgttaa ggtcttacga taggcaacca ttctttccgc caatcttctt tccagattgt
    54961 tttgagccac cagggaagaa tcaattaggg cattcacttt tgccatatct acagggtctt
    55021 ttgctaattc cagcatcagg gaatgtttga tagtttcaaa tttatccctt aaattcctga
    55081 tttcttcatc cctttcaaaa ggaggaggac caaaatgggg acgggaacct cgcatttctt
    55141 cgtttcttac aggtggttga ggatgagggt gaaaccagtg taaataaatg aaactgccga
    55201 taaaggcaag attaaaactc accgaaatca gcaggattat tattagccag cgtttactca
    55261 ccctgttctc cattttccgc tatattatcc agaattgtgt tttcaccaaa actgccgtaa
    55321 gcgacggaag tgagagtaat ggattccgtt ttttccggaa ttatgttgtt gtagctaact
    55381 ataccgactt ttacaccagc ccataagctt aagacaatag tgagcatagc agtaactggg
    55441 gcaagtttat aacgacggaa aaatcccgga ttttttgctt ccagacggtg caccttatcc
    55501 ataatttgtc cgtgaatcca ggaaggaaac tctggttttg ggaggttaga aagcatcaga
    55561 tgcagttttt gggcatctgc cagaaaaact ttacaggcag gacatttatc cagatgggat
    55621 tgaagttttg cctgaaagcg ggtatccaat tcgccatcaa gttctttttc cagatagcgt
    55681 ttggctttac tgcatctcat ttttctactc cttcttatat tttttgcttc caattactat
    55741 accattcaac tttctttatc ctgcaaaatt tttttcctta atgtgcgttt tgctcttacc
    55801 agcaaggatt caaccgcttt tatgctgatt cccaaagtag cagaaatttc cttgtaggac
    55861 attttttcgt aatactgtaa ctgaattacg gctgccaaac gcggaggcaa agctgagatt
    55921 gcatttttta gaactgtgta gtccgtttcg ttggcgttag ccgttttttc gggaatacaa
    55981 ttaaatctct ccggttccat atttatataa tgtgccttct gttttagaaa gctgagacat
    56041 ttattatggg caattttata ggtataagca agagctgtgg cttcatctat gcgatccaga
    56101 tttttgtata ccgctataaa tacggtttgg acgatatcca aggcatcctg ttcattagcg
    56161 cataaagtta acacataatt atagatgcgc ttttcattag cccttaaaaa tgcctcaaaa
    56221 tgttctttct tcatacctgt ttttacttat cattgtattt tttgaatgta aattccttta
    56281 tcattcctgt gcaaccagga ctccacatac ggtaaaaaat atttatgcat tttaatatgg
    56341 aatttacatc atatctttag tatgttgttt ttttaacttg caagaaacaa accaaacccg
    56401 aaacaaagct tcaaggtgta gccagagaac gctgattctc cgcatttttt gtaaccgtaa
    56461 cttatccaaa tttcgtgtta ttttcacgct gaaaagtggc ttcctccgtc tacacttcca
    56521 aaacaaaact tgactaattt ccctcttatc agttagcagt aacaactaag atgattaata
    56581 aaagcgtagt ttttcttgcc cgcaaatacc tgagcggacg caaaaccaga ggcatcggta
    56641 aagctcatta tctttgcctg attggtataa ctttaggagt tttgtcttta ctttgcgtaa
    56701 cttccgtgat gaatggtttc cgcttggata ttcgtaaccg gattacaggc acatttgcag
    56761 aaattcgtat ttcggcaaaa gaaggggaaa ccatcaaagg ttactctgct atttgcaatc
    56821 aattgcaaga tatgaagttc tctgccagtc ccgtaattcg gaatgaattg ttgctgaaat
    56881 atgattctgt agttttgcct acgctttgtt ttggtattga acccgatagg cataaaaatg
    56941 tctcttccct tttgcaaaaa ccggataccg gcagcaaaga aatgattcag ggtttgcttg
    57001 tgggaaatat ttctccggaa gaattttccg cttcaggcat tgctttaggg gcagggttgg
    57061 cttcacaatt gggtgtttat ttgggagatg aaattcaagt gctgtcacca atgttcaatg
    57121 ttccaactgc atttggaatg attccgcgca tccgttttct gaaagtgcag gcaatttttg
    57181 ccgcaggaat gcctgaatat gaccaaaatt tcagctttat gggcattgat aacgctgtct
    57241 tttttgcttc ttattcccct ggcgaggcag attatattga ggttaaaccc ttttcccaaa
    57301 tgcgcaaaaa tcagccactg aaaaccctga aaaaggcatt tccggacttt caaattgagg
    57361 attggagcag ttttgatccc aatctttatt ctgccatccg ctttgagaaa ttcctgatgt
    57421 ttgtgattat gctctttatg tttattattg ccagctttaa cctgaccggc aatctgctga
    57481 agatgatttc ccaaaagaaa cgggaattgg gcttgctaaa agctttagga ctgaccgata
    57541 aagacctaca aagcttattt ttagtgcagg cacttttttt atgttccgcg ggaattattc
    57601 tgggcttaac tttgggctcg cttttactgc tcatacaaaa atatacggga attgtaaaac
    57661 tgagtttggg aggaggaaat tccataattt tacccgtgaa atttgctttt acagattacc
    57721 ttttgattac tgttgttgcc tatttaatca ctattttaag cgtgctattg cctctcaaac
    57781 gcctaaacaa aataaacgct gtagaactaa ttaaacgcgc agtttagaaa cttctttctc
    57841 ctgcctatat tgctcctcag attcccttat gcttcccata tcgcgatatg ggaagcataa
    57901 gggaatgata tgggaatcgt aaaatttaca ataaagaaag ccataaatgt tattgattta
    57961 tctttttgtt ctaaaaaaag ttatgaagct aaaatgtccg tgacagaaaa aaattataat
    58021 tcaaataacc cataacgaat tgtgaatttc aaacaatgcc agtgaattat tgtgtagcag
    58081 cattgatcca ttgggttgca aaattcatct tttgcactgc aaccaaatgt atcccaagaa
    58141 atttatgctt tatatattta accgagatga attttcaaac ctatatatta ttggtattat
    58201 attggacaat cccttccttt cccgcaaaaa caggaataca aacaaaattt acccaaaata
    58261 cctgcatatc caatttaaaa atggtataat attatgctat ctaatgctat aatagtattc
    58321 atcttcaaaa tatgtctctt cactttgttc tttaatcctt gagtaaggaa aagagctttc
    58381 atctattttt acatattttc cttcctttgt ttgctgtata aatgttagca ataccttctc
    58441 ttcttttagg tttaattctg ttctcttatt tttcatgatt atatctcctt attaaactat
    58501 tattttttta cctgtttgta tttctatctg gtttttgata cctaataaat ctatttcaaa
    58561 accacactca caaataaatt tactgacctt ggggaagggt ataaatcctt tatttttaaa
    58621 atcagtatca attttagggt taggaacaag tttggcgtaa agattatatt ttttcccgca
    58681 tttgggacat tcctgttcaa tttcaaccac attaattgtt ggctttcctg gtaaaattct
    58741 tggggctcct acaggaactg cctgcttaaa tattttgttg tcttgtgtag caaatatttt
    58801 aaaagttgaa gtgctttcaa aaagaagtcg gcaaactgtt tggatcctat aaatattttc
    58861 agctaatttt gcattatcat caactctaat tatctttatg ccaattccct ccaagtcctt
    58921 aattttaatt gatctgccat gtgatctcca ttttgaatga ttcgttaatt ctgcagctat
    58981 cttttttgct ctttcaagtt tcatttgagc ttcaattgta cccaaacttc cagtttccgt
    59041 cattaaaata gcttatatta actgtctcaa aactaacttt tagtttctgg acaaccccca
    59101 tataatttat atcgtatctt ttttattctg cttctctaat taatttggag gttttctcaa
    59161 tatcattcac attttcgtat atcttcttta ttggctgtaa ttctgaaacg gaataatagt
    59221 aaattatatc ttgaattaga tcttccaata gattaattga aaatagagca tatctggttt
    59281 cttctaatgg gcttgatgtt aaagaggact tataagaacc atcatcttga taaaaactct
    59341 taattttaag gattaattct tcaactttat tgattagctg attaagttcg tttggtttta
    59401 catctatttt ggaatagaga aaatcaatgc aatatacgat tttagtgatg tcttcaagta
    59461 agatatcctt atttatatat gttagaagat aaataagcca taaattggca ttttgcggaa
    59521 tcttatttaa atgatcaaga ataaagcaga ccttgaaaaa actatctaaa tctttgggca
    59581 ttaactttac tattttatct tcatcaatta cgccttgaag taagttggac aatttgtctt
    59641 tattaatatc tagcaattga tgttgaaaat tgaggatgaa atttttggca tctattctct
    59701 tacaaatgct tttcaaatta gtatccggct tcagtatgct taaaaattta tctttcggaa
    59761 ctctataagc ataagttcgc catgcctcaa ccattccata ttggctatta ttaaagaccc
    59821 attctgcttt aaatattgca tattgatatg aaggtttata aatgtatata ataaatttat
    59881 ctttgatagg ttcacgattg cctttttttg atttagcaat tttggagacc ttgaaatcat
    59941 aaaattccaa atctgctttt tcagcatatt gaaattcaaa ctctattttt ttatcgttta
    60001 tttcagcaat ataatctggc tctcttgaga tcccgccttt tctcaaaaac cataagaatt
    60061 tatccccacc cgatttataa accttaacac atttttcggg atatagttct tttattttat
    60121 cttccagcca ttccaaaatt tctaattcag cgattgcttt tttcttcata tctttgcgat
    60181 attcaagata agtgcgggat tttatatcac tggattgggc ataaatggtt tttccattta
    60241 atttcataac ttgtcctttt taccaaattt ctgtttgatg atatcttaga gcttttactg
    60301 cttcataagc aatctctgga tatatttcat agccaatacc attgcgtttt aaatctgctg
    60361 cagcagaaag agttactcct gaaccgacaa aaggatcaag aatcgtttca cccacataac
    60421 taaatgcctt aattaaacgg aagggaagtt cataaggaaa tggtgcaata tgatttctat
    60481 tatcaccgct tccttgtggt ttcataaccc aaacatcgct tttcttgata gacagccaga
    60541 agtccttgtc caacttggat tgttcttttt gctctttagt aagatgacca tatttgttaa
    60601 agctcttctt ttccggttta tcaaattcca agatgaattc gtgcatatta ttgattaaaa
    60661 ttccacctgg atagggataa gttccaaaat gtgctcggac ggcatttgtt ttttgccaga
    60721 taatatctct tttaaatata aagccaattt tttcacacaa atctatggtt ctccccgcta
    60781 aattcaaggt tctaaacttt cctttttcta atcggacagg gaggttcatt atatttataa
    60841 atgcttttct ccctggttgt aatacccggt aaacttcttt ccagacctcg cctattttct
    60901 caaaccattc atcaatatta tgaatattcc caaggtcacc aggaagtggc tctcctgaat
    60961 acattttagt atcaaaatag ggtggagaag taatcatcag atggatgctg ttatcctcca
    61021 aatcgtccat tttcatagaa ttcttaatat atattttttg aattgtgcca tttatctcaa
    61081 gtgtatttac tttatcaata tcaatgttat tgctatgaga tttatattta agatttgcac
    61141 taaagtcctt taactctttc aggtcaaagc gcatttgtcc acttgcggca atttgcgctt
    61201 taattagctg ctgattgaca atattatgaa gcatctcaac tgttatacct aaaaatttgg
    61261 atgcttcaat agctgataga taagttctat ttttatagtc cgttggttcc attttgctat
    61321 ctcttttgct taatatcgtg actaatgcat tatagatata ataggttact tgcttcttaa
    61381 gttttcatcg tcttcttacc ttaacgaatt tacgaaaatt aaagctacta ttttatatct
    61441 aaggggtaac aaatcttttt tttgtcacct ttggtgcaaa ctgctaaaaa atgttttata
    61501 gttttaccgc ttcggatttt tttattcctg aataaattca gccgcctctc tcataattta
    61561 actaaaaatc acatataaac cttaaaaaac cgatgtaaaa tactgtcaat gaaatttcaa
    61621 cttaaaaaag caattatgat attacaacag aaattcaaag ctaaaagcta atttgctgtt
    61681 atataaaagg tgattaagct ataatgttat tctcctgtat aagcacatac tccaacccgg
    61741 ggagaattaa ctaccggtaa taacatatcc actccacatt tatgtttgct ggcacattta
    61801 tcacaaagcc atcctttgct atcccaaata caagcaacac aaacttgagt ggcaggtttt
    61861 ccacatacat aacatttttt ctcaggagga tcatttctgg ccataataat aataaatctg
    61921 cctttcgctt taagttccat ttcggatata accttcaagg taagaaaagt ggttgtcccg
    61981 aagtcgtatt cgtgataaaa ttttatccct ggatagaaaa cctcattcag cttaaatctc
    62041 atactttttt cttttaattc cggcattggc atcacggagt atgttttgtc ctcaatttta
    62101 aaggcactaa ggtgtccgca acattccagc catatatccc ttagaaaagt atccaacctt
    62161 tttagagata tatttgccgt tactaaaaga tgcatccaat attcaggcaa gtatcttccc
    62221 tcaaccaata aatgaaaaca ctttacgctt tgtgtttcat catattccaa agatgactcc
    62281 catgccggtt gcattgcttt acaagatgcc aaatgcttgg tcattgctga cttactgaaa
    62341 gttccaaagc aaaaatggca tttacctgtt gattgaattc tgggcatttc tatattgctc
    62401 ctcttttata ttttgctatt tctaataata tcgttgatta ctattcaaat gtcaaataat
    62461 attttgatga caaaagagat attccaaccc tgcttcaacg cctcaacgat attaatgata
    62521 ttagacaagg catcttacta taaaataaat aagagctact tgcgtagctc ttattattgg
    62581 gctgatcatc aatattgcct gctatttgac cagttcacat ctttcaacgc gggcaggcgc
    62641 tccagatgat tcacctagat attataatct tttttttctt cttgccaact ctttttttct
    62701 accccttacc atcgccttca tcatctgcgc tattttcttc tcttatcata aattatgtct
    62761 ctaaccgtca aggtacttaa atagtacctc tctgctacta tttctattat catgtcctaa
    62821 aatggcggac tgttttttgg gttttttcat ttttaacttt tgagataaat agcacatagt
    62881 atttagtaat tgatttggat gttattatat gttaaccata aaatccacaa tcccttcagg
    62941 ggcgaaaaac attccggtat atcgctttac ttcctttaaa ctgttattca caattgactt
    63001 atgaagatta tagttccctt acacttcttc ctgcctaaat atgggtttat tttcctggtt
    63061 tttatcttct atttcgttcg tattataaaa tcactgtaaa gcatttaaca agtttgctga
    63121 taagacattg ccgaataaaa gctaatataa tcggcaagct tttttctcgg ggctaaacga
    63181 tattcaatta acataattca ataattctta cttttttcta tagtagaata aaaatctaac
    63241 tgggatgagg ttttatgcgt tagtttttcc agaaattgca ctaaattagt ttctacatta
    63301 gtattttcta tccttttttg agctatgttg taataggttt gatcaatttc aatacctaca
    63361 aaacttctat ttagtcgttt tgccactgct cccgtcgttc cggaacccat aaagggatca
    63421 agaataatat cttcttgctt acttccaatc aaaacaattc tttctaagag cttttccggt
    63481 ttttcagtag gatgcattgt tttatgtttt tctgccgata gttcccataa attacgcatt
    63541 tgttttccat tagctaatct cactgccatt tcataattaa aataatattg ttttgatttg
    63601 gcagccaacc agattaattc tgttgatggt acaaaacggt ttttttgaat taaaggagct
    63661 gcattcggtt taaaccaaat aatatcgttt attatccaaa gatttaattg ttttaaaatt
    63721 acgccgatgg agggatgatt atgtaaagtt ccggaaatcc atattgttcc attatctttc
    63781 aaaactctaa tacattcctc aatccatttc ttgttaaatt catctatatc atcaatatga
    63841 tcccaagagc ccttgtcaca tttagctatc ttcccacttt tacaggtaag atgattttct
    63901 ccggaaagat tatagggcgg atcggcgaag attaaatcta tgctctttga ctgtaattgt
    63961 ttaattattt ctaaagcatc accattaaat aatttaatat caggcatttt cttttatctt
    64021 ccttcccaat attctattat gagcaatatt acaataatcc tcattaattt caattccaat
    64081 ccaatttctt tttaagcgag aagcaacaaa ccctgtagtt ccagtaccaa agaatgggtc
    64141 cagaataaga tcattttcat ctgacgaagc aagtaaaatt aattcaatta gtttttccgg
    64201 tttttgagta ggatgtaatg ctctcccatt ttcacccttt attctctctt tgccctgaac
    64261 aataggcatt tcaataaagt caataaaatc ccttaattgt ttcgacttac cgtctttagt
    64321 tttatgcgga ttgaaattct taattacctc ataattgaat ttccattttt tacctttcac
    64381 aaaccagcag acaaattccg ttgaatgggt aaaagttctt ttcgtgatat taggcattgc
    64441 attggtctta taccaagtta ggatattatt taatttgaag ccggtttttt ttgcagttaa
    64501 tataatttcc gctatgttat gatgtgtgca ggagatatat aaactgccag tattttttaa
    64561 tataatccgt actttactaa tccagttttc agaaaattca agataatctt catagttcca
    64621 agtgtcccaa tcctcattca ttttatagaa aggacctccg gtctgattat tttgtaaagt
    64681 taacgacttt ccagataaat tatacggtgg gtctgcatac actaaatcta tgctacttgc
    64741 agatatctct gtaaccatta ctttaagaca atcaccgcaa tatatatggt ttatctgcat
    64801 ctttttataa tcctttgttg ccaatctgca agggactttt caataaataa agcccagtta
    64861 gagaaactta ccaatctttt tgattcgtta actatatttg tatataactc atataattct
    64921 atatgagaaa atctcttacc ttttttaagc agtaataaca atgtatctaa tagcatagca
    64981 aattgttccg aagtcattgg cacgattttt tgaggtatgc cattatactc atattttaca
    65041 gaaatccaaa attgagaata agtatcaata tgaatccggg gcgcaataaa aatgcaaaag
    65101 acatcatcgt tctgatgttg aatttcaaaa tttctcaaat gcctcattac cggttgtcct
    65161 tccagcaccc attgaagttt tgatgaattt agagttacct ctataattgc tttgaatttc
    65221 ttgtaataac attctatatc cggtttattt gccggagaat gagatactgg ttcaccatca
    65281 tcatctaccg gataatttgg ttttatctga atttcgtcat tgatgatttt tagcgcttct
    65341 gttactaatt tttcaaattg ttccggctca tattctctta atttttttat atcttgcagg
    65401 gtaactatta tttcttctac tttatttaaa tcactcatta actttacttt cttaattctt
    65461 tcttttaatt ccaaattaaa tgctcttatc tgatctttat acttctctaa ttgattttta
    65521 tccaaatcgt gaatttccat atctaaaaat gtctgttgtt cttgactaat ttgtagcttt
    65581 ccttccttaa tcaaattatt agtatcatat tttagggttt gaactacctt ttctaaatta
    65641 tctcttttct cccagggtaa aagtggctgt tcaatatcag aaagataatt caaataatct
    65701 gttacagttt caaatttaat agatgctccc ttatacatat ctaacaattg ctctatttcc
    65761 acttttctgg aaggttcaag atcaataatc caattggagc ccaagggatc cattgagacc
    65821 ttaaaatatc ttgtaagccg aaaatatctc attatgttat cgccataatc aaacaaatta
    65881 tttatcttat ttgctggtat agtcgttgtc tgataaaaca ttttcacaaa gtcattaata
    65941 tattcatctt tgtttttcgc ttttctaaat tctaaaattc gttgcacata ttcatctacc
    66001 tggtcaaatt tgataagagt aggaacaaat aaattgaatt catttttatc taatcctttc
    66061 ttttctgctt ctccgttgat tttctttatt aatttcatta cggtgattaa cggcattata
    66121 tcaaaacctt ctttctcgga aaagtccgca ctccagggat ttggaaactg aagcttaagc
    66181 aaacttttaa aaaatatata acctatctcg taatcatctg ataaaaattt atttccaaga
    66241 tcagtaatta ttattggacc atatccttcc cttgctatag aaaaaccaag cttatttaaa
    66301 ggattaacag attgccttcc cctcattggc ggatcctcat atttttgcat aataaagatt
    66361 tgttcagcta ctttgtaagg gatttctgtc tctggttctt cataatattt tagataatca
    66421 acaggaatat ttagcggttt atatatacgc tctttaatta aaagcacttg atattttatc
    66481 tggttagaag tatcaaagct ttgaccttcc aattgcttta atacccgcaa aaaatctctt
    66541 aaccgttcag gatttctcac tgtcgttgaa attgaccaag gttttctcat attttcacct
    66601 cttataatac acaaatttca tttctcactg aacaacaaat aagtcaaggt atttctaaat
    66661 tgcagttact tcgctgcgga tagcttattt cccgatacag aattcgctga agattttatc
    66721 caagaggtcg tcggtggtga taactcctaa aatttcttcc aaagcattgc tggcggaaat
    66781 aagttcaaaa gcaatgaatt cgtagccctg attttcttct atagattgtt ttgcttgctg
    66841 taatgactgc aagcatttag atagagcaac taaatgtctg ttattgatga taattggttt
    66901 attgggcaat tcagtgttca gcattaaacg cttggaaatt gcatcggtaa ggtctttcaa
    66961 gccattttcc gttattacac tgcaatatat cccgcgagag atttgaggtg tattttcttg
    67021 cagcaagtca gctttgctga aaacaacaag ggttttggaa tgaagatcag gaggaagaga
    67081 agagggagaa agtaaatcgg aaaattgtag atttggatta gttatggtag ttgcttccac
    67141 aagataaaga ataaggtctg attcctgcat caattcatag cttttagtta ttccttcttt
    67201 ttcaatgtca tcaggaaatt ctcttagtcc agcagtatca taaataacta tgggaaagcc
    67261 atttagggat aaatattctt caagatagtc cctggtagtt cccggatggg gagtaacgat
    67321 ggctctgttt tgttgcaata aggcattaaa aagtgaggat ttaccaacat ttggtgcacc
    67381 tgttaaacaa attttaattc cttcccgcaa gcgtcttccc tgttcacctg tacttttcaa
    67441 ttcttcggcg gttttgataa tatctaaaag ttcttgatac agtgcattgg aatcgggtaa
    67501 aggtaaatcc tgatcggcaa aatcaattgc tagctcaaaa cggattcgca gttccgtaat
    67561 gcgggcaagc aattcttgta agtatttact cagaaaaccc ttaagttgca taagagctgc
    67621 tttttctgct tggtttgcct gggcttgaat aaggtcatta actgcctctg cctggcttaa
    67681 atccattttt ccgtttaaga aggcacgtaa agtaaattct cccggttttg ccaaacgaca
    67741 ggaaagaagt aaggtctgaa gaatacgatt tacaagattg ggatttccgt gacaggaaat
    67801 ttctataaca tcttcacctg tataactatg tggttctcta aaaacggaaa taagaacttc
    67861 gtctatcggt tcaccggttt ctgcataaaa ggtgccaaaa atcaggttat gcgaaggtga
    67921 agaaagaagt ttttggcttt gggaaaaatg attggaaaca agctcaatac agcccttgcc
    67981 acttaagcgg attactgcaa tggcagaaaa tccaagcgga gtgactggag cacaaataac
    68041 ttcgcttatt ttactcattt agtgtttgaa caatttgttt atgaagcttg ttaggcgtaa
    68101 aaccaatttc atcaagaagt tcagaatttt ttccttgagc aataaagtga tcgggatagc
    68161 caaaattgat aactttaact ggttcggttg caagcaattg agctattcgt gctcccatcc
    68221 ctccaataac ggcattgttt tcaaaagtga agatataatt gcaggtggaa gccaaagaca
    68281 aaagggtatt ttcatccagg ggtttaacgg aaagtaattg tatcagttga gcaggaattt
    68341 tatctttagc cagtaagtta tacactgcct cggctgtaaa aaaagcatct ccgcaagcga
    68401 ctaatgcaat ttttccttta ttggagtgaa ttatggcagg aaagggatta aaattggtaa
    68461 aggatttatg aacagaagaa tgaattgctg ttccacgagg ataacgaata gcaacaggac
    68521 cttcctgata atttgcagcc cagaataaca ttgcagaaag ttcttcagcc gtagaagggg
    68581 ttaaaataat catattcggc acaaaattca agtaggagag atcaaaagca ccatgatgcg
    68641 tagctccatc ttcacctaca agaccagctc tatcaataca aaaaacaacc ggtaatttag
    68701 gtaaagcaac atcgtggata atctggtcta aagcacgttg cagaaaagtg gaataaattg
    68761 ccacaaaggg tttaagtcct ttagtggaca ttcctgcggc aagggttacg gaatgctgtt
    68821 cagcaattcc aacatcaaaa aagcgatcgg gataggtctc ttcaaatttg gttaaaccag
    68881 ttcctgcagt catagcggct gtaatagcga ttattttggg gtctttttgg gctaaagtga
    68941 ttaaggttga acccatcaat tcgctccagg tttgttttcc gttgctgatt tgtttaccgg
    69001 ttttgaggtc aaaaggacct gttccatgaa ataattccga attcttttcc gctggaggat
    69061 aaccctttcc cttttgggta acaatatgga tcagaacagg accaaccata aagttcttta
    69121 ctcttttgat aattttcagg agatgctcaa tatcgtggcc atctatcgga cctacatatt
    69181 tatagccaag gtcttcaaaa ataatgttgg gcaccaggat attcatcatt gattcttcca
    69241 gtttctgagc tccataaata aaactgcgac gaatattggc tggtaaagtg taactgaaat
    69301 cccaaacctg ctttttaagg acattatagg atttactagc aagcatccga gccatatatt
    69361 tctgtaatcc gcctacattt ttggagatgg acatttcgtt atcattcagg ataacaatga
    69421 atttatcctt ttgcaggtga cccccatgat ttaatgcttc aaaactcatt cctccggtta
    69481 aagcaccatc tccaataaca gcaatacagt gaccctgttc attgtttaaa tccctagcac
    69541 aggcaattcc taaggcggca gaaatggaag tgctgctatg acctgtagta aaagcatcat
    69601 aaggactttc ttcacgattg gtaaatccgc tgagaccttt atactgtctt aaggtatcaa
    69661 attgagcatt gcgtccggtt aaaattttcc agctgtatgc ttgatgccct acatcccaaa
    69721 cgattctatc tttaagcgga tcaaaaagat gaagcaaagc aattgtgagg tctacagttc
    69781 ctaaactggg agctaaatga ccgccagttt tggagacaac ttctacaatg cgagagcgaa
    69841 cctccttagc caggattttc aattcgctaa cggttagagc ttgtatttgt ttcggctcgg
    69901 ttatttgttc caaaatcatt ttttctcctt gccgcctgct attttgccct tgcctcctct
    69961 gtttttacaa taataatttc cacaatcatt aaagcgaaag cgatggcaag caaaattttc
    70021 cagaggtcgt gacccaagcg ggaaaagaaa agtttatctt cccatttttc tcctaagttt
    70081 tttacacctt tggggaaatt agttctggct tcggaatcgc tgtagtcaat atttattgcc
    70141 atagcataac tgcgaggagt atcaggttca aaaatataaa ttccgggttc ggtagctaaa
    70201 taacggcggc tggtgataat ttcacctgtg gggagtttca atctgttaaa agtaagaacc
    70261 tgaccaattt tagcatttgt ttccggaatt tcagaaccgg caatataatc taatgtgcgg
    70321 aaagccagaa ccggaaaagc cgggtctata aaaaaaggat tattgtccgc agaaatattc
    70381 cagagccaga gtgactgctt gccgttgatt acagctaaag gaaagttttg agcagaaatg
    70441 agaactccgg cacttttgtt tactgcctgc cagtagtcag tcagaatagg atttttaagg
    70501 ggttttccgg aaacgaggga actgatatag tgatgggcat taatgttatc aatagttatt
    70561 gacttttggg tgcggtcttt tatttctaag ccaaaagtgt tgtttagata ggacttcagg
    70621 tcggcagaaa ggttgtttcc caggcagaaa agaatacctg atttacggtt ttgtaaagta
    70681 gtaaaaatct caagtaaacg agaagttaaa ggaccacaat tgtatgcaat aaaaagttga
    70741 aatttatcca ggtcacttaa tgagagggca tcaggagaaa ttacttgagg agtagaagag
    70801 ctataaaccc ttaaaacggt agccagaata ggaggtaaat tgctactttg agagataact
    70861 gcaacccggg gtttactgta aaaggggaaa gcgaaatagc aacggttatc cattatctgg
    70921 cgttcatcat taacttcaat atatccactc tgccagccat cagaacgaag ttcaacagga
    70981 atagtttcgg tgatagtgct ttgggaaggg atagagataa atttttcggc aagttttata
    71041 tcgtttacca cagctttaag gagaacatct tggcgtttgg tgctgccata attggcaacg
    71101 gtgaactgaa ttaattgctg attgtgtttt tccaccagct ggggtaaagc attggcagaa
    71161 ataactgcca agttttcata ttcagatgtt tcaggtaagg gaataagagc aataggaata
    71221 ttggttttaa gttctgcagg aggaattctt aaatcgctga taagatatat ttcccgattg
    71281 ggcatttgac tttcggcaag tttggtttct gccaaggcaa gcatttcttc caaagagaga
    71341 gggtggaaag tgatttttaa ttggtcaatc agcgtttcag gcaaggaacc ggcataaatt
    71401 tgactgtgca aaaggttcca gttttcatcg gaactgacag gaatgaggcg atcatcggga
    71461 ttggctttgg agtttatttt tttcagagca tctttggcat attggaggga actttttcct
    71521 ctttcagcat agtccatact gtaagaagta tcaattaaaa cggcaattgc cgtaggtgga
    71581 tgttttccgg aaggttttag tttcggggaa gagagcattg gacgagaaac agctaaaacg
    71641 accagcagga taatcagcat cctgataatc aacaaaagta tgtttttcag cttgctgcga
    71701 cttttctcct gttctttgct caatttgatg aaacgtaaag tagggaaaat aattttttgg
    71761 ggcttttttt ttgctaacag ccaaatcagc agaggcagaa tggttgctaa ggcaaaaaag
    71821 aggagagaag catttaggaa agaaagctga aacaattata tccccgcgct taaagaaaaa
    71881 agatagtcgc tatcggagag cttcattcct atgttattca gcgagaaatc aaagcggaag
    71941 tttttgtaat aatagccgcc accaagagta aagttaatgt tatccgttcc gtaaaaatcg
    72001 tgactgtaaa gaccaactct gaggtcaaga gaattacggt tttgggcagt tgagtaattg
    72061 cttttgccca aattaaaggt gtagccgtag ccaaaatgaa aagtggtttc gggttcttga
    72121 gcaattttgc cttgtatacc ggcaaaataa tggctgtttg tgccttcaga accaagtccc
    72181 aaagcggctc ttcttttaag ggttacggaa tcataatttt cccaccagag catagacagg
    72241 aggtcataaa aggaagcaga ataaacaata ttacccattt tgtaagtgaa accgaggtct
    72301 ccggaaatac ctttggcttt atcatcaata aagccatcgc ggattaggtt gtaaccctct
    72361 ttatgctctt tcaagtaaac gaggcgtcct gaaaggtatt taaggttaat tccggcagca
    72421 aatttgggat aattttcgtc tctgccaccc caagaaattt ggactttatc cattttataa
    72481 tcgtaatatt cactgttcat tccgttgttt gtgaactgac tgatatggac agcagaaacg
    72541 ggttgataac tgaaagagac aggaccggta ttgagcacga agtatttgaa ctgcttatct
    72601 ttcagggtat tgcttaagct aagagcttca tagaattcaa gatcttcctg agtgcttaaa
    72661 aggaaggaaa gggaaagcga gccaagtttg ttatctgcca ttaaagcagg attgccgtag
    72721 gcgcagaaag ggtcttcagg attggttaaa tttaaggcac ctgttcccat agcaataggg
    72781 gagacattat tggcagggag ggcgtaatca taagaagtta taggtaaagg agttacctgt
    72841 tgagcaagta ggagccaagc gggagagata agtaagatga tataaatgag tgacttaggc
    72901 attagaaatt ttattttgga catcagttga accctttttc tctaagataa taataagtcc
    72961 agttagctaa atagtaaccg gcaatacctg ttaagatacc aaaaacggca tcaatgaacc
    73021 agtgatagtg acaataaacg gtagcaagag aaaggaaaaa ggtaatgaca gtgcaaatat
    73081 agccaagcgg acgaatatat tttagggcgg aaatagttag aacaatggca atggcaatat
    73141 gactgctggg gaaagcacct cccaaatgat tggaagtgcg atagataaag accataatat
    73201 gagtgaaggg accaccccga taggttttgg ttaaagccat agcttcagga agaaaacgtc
    73261 caccgataac aggcagaaca gaatagatgg tataacaaca atagaagaca aaggtgagat
    73321 tgaagattac ttccctgaaa gcatcttttt gagtgaaata gagatagatg ggaattccag
    73381 caatcatagg ataatagcaa aaataggcaa aatgaaaaag ttcctgaatt gcccaatagg
    73441 aatagagttt tccccaaaca agagaaggca ggtaaccaaa gataaattga tcaattccca
    73501 taaaaaaggg atcgagccaa tcccgaaaga taatacgatt gaaagcgtga ccagaagtgt
    73561 aaaaatagcc gaaaaggaga acaggataga gacctctgaa aaaactgaga atttgataac
    73621 gcttggaagg gttttgagga tcgtaagacc atccgggttg tttttggagc caggcaagaa
    73681 aaagaactcc tacaaaaata gcaagataaa cagggatatg ttttataact tcaggagagc
    73741 gagtgatacc aaaacacata tacagaagta tccatccgca aaagataagg gtaataagat
    73801 caatggcaga gaggaagaaa gatgtttttc tatttgccgt tatggaggta tccgtcattc
    73861 agaacccgct atttcctatt agtaatggct ttatcgtaaa ggcggtaggt tttataaatt
    73921 ttggcaccga gtttttcagc ggcattaatc atcgggatat tgttttccag aatccaggaa
    73981 aattcgctgc ggtaatagcc cttggggagt ccatttttaa aagtatgata atagagcaaa
    74041 gtatcaattc ccttaccttg aaattccttt ataataccca tagtgataac gcggacggag
    74101 gtgatatgtt ttttagcttg gagagctttg ataattgtga taggatttac tttaccctgc
    74161 ataactttta agacctcatt gtaattgggt aaagttaaac tgaaaccggc aagctggcca
    74221 tcaacttcag caataaaaac catatccgga tcggcaatag ggagtaattc agcaacgatg
    74281 tgatcaaatt cagcttttgt cataggaaca ttgccccagt tatattgcca ggcacgggta
    74341 taaatttcaa agacggtttc aatatctttt ttgcgttgtt ttttatcgta ggacagggaa
    74401 tggatttgaa cattagtgcg tttggcaatt atttctgcca ttttggcaac gcgttcggga
    74461 atggaatgat attcactaat aaaggcatag aggtccattg ttttggttaa gccgtagttt
    74521 tcgtaaagtt ttggataata aggaaagtta tagggcatca taatcatcgg gggggaatca
    74581 aagccattga ttaataatcc gcaatcctga ttgacactga aattcatagg accgcggaga
    74641 gaagtaaaat tacgatagcg attccactca aaagcggcgt caaaaagggc attggcaact
    74701 tcctgattat caatacactc aaagaagcca aagaaaccga tattttcgtt atgttctttg
    74761 ttatgctgag tattggaatg agcagaaatt cttcctacgg gttctccatc tttaagagca
    74821 agaaaaagtt taacttcgga atggtaataa tagggatttt tcttggggtt aaagaacttt
    74881 ttctggtcac caattaaagg aggaacccag ttgggttcat ttttgtataa tttaaaagga
    74941 aggagaatga agtccttcag cttttttttg ttatctacag gaattacatt gatcattgcc
    75001 ttacctcatc aggggattta gaattctccc agttttttag aaacgatgat atttaaaata
    75061 cccatcatta tggcgttgcc aagtaaaaga gtaataaaat agggagggga agatttatgg
    75121 tagtgaaaca aggtgtaaaa aataatatat aaaataaccg gcaaaatcac aatgagatat
    75181 tgagcaaacc ttatttgctt aaagtggaga acaataagcc aggcaataac agcagaaata
    75241 agagttggaa gaaaagcaat gtaagcaagt atacccatat aggtaaaaat tccgcgtcca
    75301 ccccgaaaac gatgagtaaa aggcaagcag tgtccaataa gcatactgag tccataaatc
    75361 aacattaggt tttccgaata gagaatagtg aaatcgggat agttaataac tttgatatca
    75421 ataaaaccca ggagaaattc cacaaccagc aaaaacagag atgctttagc tacatcaatg
    75481 gctcctacta aagttcccag tggcttgctg atattagtat aaatattttc agtatcagca
    75541 agaccggttc caattttata aacattgaga gaacggaaac ttttggcaac gatgcgagca
    75601 gtggaaaaac agccgtaaaa ataagcgatg ataataataa gaataacaaa aaggtagatc
    75661 attcattaac tccatagcta cgctctccaa aaatggcagt gccaatgcgt aacatattgg
    75721 aaccttcttc caatgcttga acatagtcgt tactcatacc catagagaga tattgcatat
    75781 ctacattagg aatagcaaga gctgttattt cttcaaacag ggatttcatt cgggcaaaaa
    75841 gaggtctgct gacttcaggt tctgcgagca taccgatagt catcagtcct ttaatatgca
    75901 agttagaata ggttgagatt tggcaaattg cgtttttaac ttccacgaag gaaaagccgt
    75961 ttttggagaa ttcttggctg ctattgattt gaataaggat atcctgtgtg cgattggttc
    76021 tacctaaagc cagatggagt ttttttgcca gctccagaga atggaccgat tgaattaaaa
    76081 agggcttaag ttccagaacc tgatttattt tgttgctttg gagatgaccg ataaagtgaa
    76141 aacctttgta aggaacctta agaaagggaa ttttccttcg tgcttcctgc accctgtttt
    76201 cgccgatatg ttcaattccg gattgcaaag cggtttcaat tatttctaca ggatgggttt
    76261 tggtaacggc aaccaaagta atttcatctt tacgattcaa acgggacaaa atatccgcaa
    76321 tgttatcctg caggtgttta atgttttcgg ctatattcat ttcagaagta acatcttgcg
    76381 ttggaatgtt tttccttcaa tgttcaagcg atagaaatag acaccgctgg caacttttct
    76441 ggaaaagtcg tcatatccgt tccaaacaat actatgattg ccttcactat gaatagcgtc
    76501 tatcaaagtg cggatttttt gacctttgat attgaaaatt tccagtttgg cttgaacatt
    76561 ggcttttgta gtgtagcaaa tagtagtttc cggattgaag ggattgggat aatttcccag
    76621 taaagcgttt aaggcgggaa tattatattc gtcggaagaa tcaataaagc cggtggaagc
    76681 aaaagaaccc tgccttaaag cacttccccg ataggtgatc cagggaccta aatgggcagt
    76741 attacgacgc aggttaagca ttaaaacacc attagaaaaa cctaatacca gtttggtttg
    76801 gttattgtta tcaaagtcaa ctaaggttgc tggtgtgctg ccattgtaaa tattgataaa
    76861 ggggaaaccg cttaaaggac tgccatccgc tttgtaaaca taaacagaat tcatataatt
    76921 atggacaatt atctctaatt gagggttact atccagattg acaagaagag gaggacaagc
    76981 aaaattttct ccaatattta caggaaaacc gggaaggtca ataccggaat cagtgaaggc
    77041 atagaggata ccgttgagag ttacacccgc aatatctgtt cctgtattat cagcagtaat
    77101 atttcctaca gcaaaaccac cggtaatgtg agtattgata ttacgggtgg caatgatgtt
    77161 tccctcggga gaaatgatat aaatatccgt gggtgtagaa caaacgattc tattggtgtt
    77221 agtaatagtt ggaaagccgg ttacgggacc gtttaattga acaggaaaac cgtttcggac
    77281 aatgccatta gctccaacga cagttaaaag accgttagtg gagccggcta caatttcaaa
    77341 agagccattg ccgtcaaaat cggcggcagc aagttcgcta ttgataattc ctccaagttc
    77401 aaaaggaaag ccattcatta gttgtcctga ggaagaaaca gcatacaatt ttccgtttat
    77461 gccaccgaca attgtttcca ggatgccgtt gccatctata tcagccaaca taggagtgaa
    77521 taaaaaagtt gtatctgtct gataattaaa gatgatatct ccgtttaacc gaagagcgaa
    77581 aatatttcct gagcgagtgc agaaaaccag atcgttttga tcatcactat caatgggacc
    77641 gctagcaaaa ctacgattga ttaacattcc tgaaggggct tcaagggtat taagcaattc
    77701 tccgttacta ccaattatat agctgtttcc ataaacatct gcataaataa tttcaagacc
    77761 gggactggaa ttcaggtctg ccacaagagg tgctgactta ccattaacta ttgtttccca
    77821 gggaaaacga ttgtcaataa gagttatctg gaagaaaata ggaaatctat gtgcaccagt
    77881 tgattgaaaa gttataggat gctgagaatt aagcaccaga ttgaaactgt atattccaaa
    77941 actcatatca tcagaaagct ggagacggaa ataaatgtta gcaggacttt ggtttccggc
    78001 aagaatttga gggataataa tatcatcgga gagcaattcc actccgggag ggagattttc
    78061 tatgtggaga gaaacattgg ttgcatcagc ccaattgata ttattggaaa taagaggata
    78121 gaaacggata atttcaccgg gattaatgat accatcatta tcgccttctt catcagagaa
    78181 agtatagctt tccaaattta ataggggaag attgggatca taaggtttaa tagcaataga
    78241 agggtctccg aacaggacta cttcgtaata acaccagcgc ataacatcat tgcttaaggc
    78301 agaattcagg ttttgtaagc gggaaaaggt taaggcatca cctaaaacag gacaattctg
    78361 ttgaaacata cctcggaaaa actggcggtc ataaaattga gaagcaccat tgatgtttcc
    78421 aggactatac catccgtaac gagtgttgcc aataaaagaa aataaagcgc cagcggcagt
    78481 aacaaaatgc tcaccgatag cttcattgtt tccggaagtg cgttgatcaa aagcagcggg
    78541 ataacaaccc tgagaataga gaaaaccata ttccgtatta gttaaacttt caacggtagt
    78601 attggattga ccgataagag aaacttcgtt ggaatgaccc atatgattca taattcctgc
    78661 accgttactg attgcattgt aaacattatc agcactgtaa gtgccatctc tctgataaag
    78721 ggtttggaga tagtattcag caggaagata ttgagctaca tcatctttat agtcaccacc
    78781 ccaggttaag ggattattat tcaggttttc acccataaaa acagcatagt tattgctgta
    78841 ggtattatta tccacataat attgaatttt tctgaacata ttattgaatt cagtttgggt
    78901 ttcagcagga aatctaccga tatgaacttc cggaatcagg tctgtattat catttgtttc
    78961 accccagatt tgattttgat tagcattcca attgccatct aagttgctaa aatatatatc
    79021 ggtaggcatt ctagcgtcaa ctgtatcacc aacttgacca taggcgcctc gttccggaac
    79081 aatttcatcg tcaccaccta aaataacata ttcaagtggc tgggaactat tagcccaggt
    79141 ctgataagca tttataatga agttacggag tttttccgca ttgtcaacac caggataggc
    79201 agaaacgata tcttccatag agtaaataga attggaaaca cctttggcag aacgccaggc
    79261 agaatattca gaaaaccagc cgatcctggc attattggta ataattatca tttgtttggg
    79321 tgtggaaagg tctataatgc gattaggaac tggaacattg cgataattat ttgccaattg
    79381 ataggaggga attttatccg gattgcagat aagggaattt attatttgga gagttttagt
    79441 gcctggagtg taaaaatttg cctggtagca agcttcatct tcagaaaatt cactattaat
    79501 agaaatgcta atctgggagc tgacatacag tttttgcgtt acaggattat agcgataggg
    79561 atatacatta aacaaggcaa tttgatagcc ccgaaagtat tgtgttccta aaaactgcca
    79621 ctgctgagaa ggataaagac ggttttccgg aattgtattt gcaggtggaa aagaatttgt
    79681 aaatacctga tcagaaattg gcaattgagg tccggcaaag gcaaaagtaa tattttctgc
    79741 aaccagttct gcagttgata actgaacagt ggaattaaga tatctatgac caaaaggaag
    79801 cagaatattt accggagtaa aaggcaaaat cggttctccg ggatttatgg ttacaggaag
    79861 ttcttcagcc ggttttttat tttgccaggc aaaaggatca atatttattt ctgtattaaa
    79921 agcagccaaa ctgagacaaa tcagaatggt actaccaagg acaaggacct ttttcatagt
    79981 ttatatccat cttttttatt agttacttat aaaaagtagc aagatgtatt ccttgatcat
    80041 aaaacatatc aattcgtcaa gtattttcct gaaaatagag tattaaattc cgattcggca
    80101 agtggcaaag gggaatttgc cacttcattt tctttaagca aggaactatt tatttgctct
    80161 tttactaaaa ccgtctaatt tattattaaa agaactttta cttatgagaa aggagtaaac
    80221 tatgcaaata ccctttaata tgaaccggtt gaaagatacc gaaatccatt atccgcaacg
    80281 tttttccaat ttgttaacca aaaattacgg acttcttttc tacaatgaag gaaacaaagc
    80341 ttctcaggaa agcaatcatg ccgtgatttt agatttgata ggggttgaga gttctttgag
    80401 ggatatagag tttttctata aaagcaaagg tattcatccc tgtatctatc ctgcattaac
    80461 taataaagaa ctggagattt taacacccct gctggatgag cataatttta ccctgaagat
    80521 caagcaaagc gatttttttc tgcatgatca cgaaagcagt ttaagggaat ccgaagaact
    80581 gaaaatcgaa cgcattcacc aactggacat tgatattatg gaaaccattg ctatagaata
    80641 tggaggtgac tggacaatta gggtagtgga gcgtcatcta actcaccctt cctatcattt
    80701 tttgggtgga tattatcagg gtgaactggc aacaatggct tcagttagta tctttgccgg
    80761 ttacagcaga gttgatgatg ttttcaccag agaaaaattc agaggaaagg gttttgcagg
    80821 aggaatgatt catcatttaa taaattacca ccggaatatc tcacagaact atctctacct
    80881 gcaatcagat cttccttccg ctattaaaat ttacgaaaag gcaggttttt cccgtctgcc
    80941 caaggatttt caattttggg tggcttataa agaacataat tagagtaagt gctgaataat
    81001 gcatcaggga acagaaaggc aattttacag tatagggtaa gaaaaccggg cgttgcttca
    81061 aagaatataa ctttcagatt gaaaatgcat aactattttt tatcttctgt ggattcctac
    81121 tttcgcagga atgacatagg acttatcatt caagaagtga gataaaaaaa tcattggaga
    81181 agaatatttg caatccaagg ttaaagccaa atgtgctttc ttcattttcc ttgtattcat
    81241 caagtgcatg ttcataatca tctccgccat aatctcctaa acccatttga tatctgagtc
    81301 cgaaatccac atatacattt tcaacaatag gcagaacata tccagctttc aagccaagat
    81361 acattgcatt gcatccacca tcataatcac ttactttcca gctgtttgtg ttatagagaa
    81421 aatccaatcc cccgtaaatt tttcctggtg ccataggata gaaatatctt ccaccgattc
    81481 cgatacccaa agcagataag ctattttctt cattagaatc gcctttgctt tggccttcat
    81541 aagtatagtt aataagcaca tcggcagaaa ggttatccat aacaaaatat cccacctgag
    81601 gtgtaaaact aaacatagta atggcttttt catcagaatt gggtttgtga gaagcgaaag
    81661 aaattgttcc tcccggatta atcgttcctt tcgtgaatgc aaaaacactt gaagcgataa
    81721 taagggcgag aaggataagt aatccttttt tcattttaaa cctcctggtt tttatatatt
    81781 taatatattc ccaatcggat attttgtcaa gcagaaaatt acaatattat cattttttag
    81841 ctatatttat attttcagtt agatgcataa taccagtaac aaactgttaa atataggtat
    81901 taagtaaaag ataggaacta taaaaagttt tagggttgcc ttgaaaaaag atttatttct
    81961 gctttgagcc aaaaccaccc tcaaacccgc aaaaagatag agaaagaagt taaaaataga
    82021 ggatagggtt aaggattgtt gccttccttg agaaaggttg ttttaactca aggctaaatc
    82081 caagaagaat tattatgcct gcgtttggga ttagtgctgc gggctaaaaa agaaccctgc
    82141 tgaattgttg caaaatcctt tccggcaact tgatttcctt ttaagtaaat tgctttaaag
    82201 gagccggaga gttcaatatc tttaccttta ttacctttta ctatggttgt agcttcaggg
    82261 tcgtagagga caaaatcggc atccgaacca gggtctaagg agcccttttc cggatagata
    82321 cctaaacgtt tagcggcatt ttcactggtc attttaaggc aggtggcaag gggaactttt
    82381 ctttttttcc agagttcgga aagcacccaa aggtatgaat aattcattag ttccggattg
    82441 caaccctgga agagttcgca gtaattggga ttttttactg gaggagcaac gcaattggaa
    82501 agcaggtaaa ttttattgga gcgcagaagg tcaaaaagta aatcggcaaa atcagcatag
    82561 tcacacagga agggagtgct gaagacatca ggattataaa gagccatcag atcagaaata
    82621 caagtggcaa aagaaatatc gcttcttttg gaaacgctat taataaattc aattgtggga
    82681 taggaagata ttctaggaat gtgaatagga ttttcctgca ttctgcgtaa gattttttta
    82741 attgcatcca gctgagcaag aggactaaat tcctgataac tttcatagtc ataaccttga
    82801 aaggcaaagg aggtatcact ttcatagata tcaaaaaaca aatccattat ttccgtgaaa
    82861 gttatatcag gaatggcagg attgggagaa ggaacagcta aagcaatgcc tacaatgcct
    82921 ttactccaaa tttcctgagc ggattctgcg tgataaggat aatcgccaat atcaatatga
    82981 ccccaaagag ccagagaagt aaaagaattg gcatttacca aagcaatctt ctttatgaga
    83041 gccgaattat tgaaaacagg gctttgagta tgatagctaa gttcagcgag ggtagtccaa
    83101 ccgccttcta aagcaatttt agttatttta gccaagtcag ttgccgcatc agcgttttta
    83161 gctaaaatat gggtatgggg atcaattcca ccagggagca ttagtaaacc cttggcgtca
    83221 attatttgga catcttcttc cacttcaatc tcatcagtgg agagcttgat aattttatct
    83281 tcaaacagca cattcactgt tttcagggct gttgaagaag tgggtaggga aacggattta
    83341 agtaatttca taatttattt ctatttgaac caaagctgat ttccatcttt gttggaagca
    83401 gtttttggtt tcccgtatat tattattcgt ttcatttatt ttattctttc agcagttttt
    83461 gcatagtggt gtttagttct tccagagaga cattatcccg caaagagctg aaaattcctt
    83521 ctgccttttt ccaatatctt tttgctgtag gtatgttatt ttccgcagca gcaatttgag
    83581 ctttcaacca caaacaatga gccatacccc gaaaattgcc aaagcgataa tcccaatcct
    83641 gagctttatc ggcatagaaa ttggctgttt cataatcagc agttctataa taataatagg
    83701 caagagcata ccaggcagaa gaaagcagtt cggggtcatt atattttgct tttttctgct
    83761 gtttttccat cttttttgat aaatttatca gttccttaac ctgtttttct tcagcaattg
    83821 ccagatagct atccgcctgc agtttatcta tagcaagttt tagattaact tcagcaggat
    83881 aatctatttt gcttattgct ttttgcgata ttgcagagta gttattttcc agctgatagt
    83941 tataaagttc cagaagcaga agataataag ccagttcagg agctgtattt tgaatcattt
    84001 cagccatttg gtttttgtat tgcagataag ctttttcatt attattttcc aattctattc
    84061 ttgccaaacc gcttaagcag gcaagcatac ctttgatatc ggcaaaagaa cgatagtttt
    84121 ccgttaagaa aatgtaactt tcccgggcag aggaaaaatc cctgttgttt tccatttgca
    84181 aagcaaaacg caaatgatta tcagcatcta tttttgcctg tggttcaacg ggaattttta
    84241 cagtagaaca ggcagttaga attagcaaaa ttaggaataa ccatttacgg cacattggga
    84301 acctgatgta tatattttgg tgaaggcata ttttgttcag cagaaggact aatccctttc
    84361 cgcaaaagag gattgttatt aagtccttcc aaggtcttac gggctttagc caaactgtca
    84421 ttcaaattac ttaacagtaa acgcaattca ggattattta ctgtagctag aattttcacc
    84481 agttcagcag tagcggattc cagattattt aaggtttccg aaaggggttg taaaatttta
    84541 tcctgctcgg gatcaagcaa ttttatcaaa agagaatcgg gttttttata attatcaata
    84601 gcagtttgag cactcgctaa aataaattcc agttcattca tgcggttatt caatccttga
    84661 gtgagttctg ccagattatt caaagttcta agcaaaacac cactttcggg attgttatcc
    84721 tgatttaggt tggcggttaa aattgaaagt tctgtagtta tttcttcaac tttattcata
    84781 ctggcatttg ctttttccga agcatctgcc aaatttacca gaaaacgggg aatggaacct
    84841 ttatcggcat tgtaatcctc attgagagaa gaagaaagtt gagctaaatt atccaaaata
    84901 agggaaatcg gatcactggt ttgaggagct atcaatttta actgtttttt accctccgga
    84961 aaatcggaag aaagtatagt acttttatcc tgtaagtgct ccctggaatt gggattttga
    85021 ataaattcca aagtggtctt tcctattatc ggatttgtat ttcgtgctaa aatggaaccg
    85081 gtaaccatta tatttttata gcgattaaga atataaaact ccagcccgat attgccatct
    85141 tccataagcg aaaaatcacg cactcttcct acctcaaagc ccttgtataa tactggagtt
    85201 tgagtagaaa ttcccattgc atttgccaaa gtgctgtaat atacatattt cttttccaga
    85261 aggttttgcc tgatagcaat aaaaaccatt gccagcaaca ataataaaac tggtatgctg
    85321 ataaaggcaa aaacaatctt tcggctgtgt ttgatcttat ctatcatttt ttaaccctat
    85381 tatggcttgt tattataatt ttaaagtaca gttttttgtc aagcttttaa taggggatac
    85441 cgagagaggg ttttttttac aaagagagaa agagaaaaag agagggattt acaaggagaa
    85501 aaagaatgat tatttaaata aagagaaaaa agatacgact tttttgtgta gctggaggac
    85561 accgttcatc cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    85621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttgtca
    85681 ttcctgcgaa agtaggaatc cacaagagat aaaaaaatat ttatgtattt ccaacagaga
    85741 tttatattat tacttaatca acaaatagat caagttaaaa cctgtttcca agtgataata
    85801 atacttcatt ttattcggtg ttctctgtgt tctgattgtc ttcgggaaaa aaataatact
    85861 aaccccggct atcagttctt cattattaaa atcacaaaaa agaacttgac tgttaaacag
    85921 tatattcaat aaaaagaatt aaacaagata agacaggatt gatagagatg aaattcaagg
    85981 attcaagtca attgacaata cctattatgg aagaaatgcc gaaatttaat agtgtagtaa
    86041 tattaaatca ggaaaagttg atagaggttt tggaagagat tgaaaaaata gataatgtga
    86101 acttgatgaa aaacagaatt ctcgcgttaa aagctattat caataattat aataatgata
    86161 gagaaggatt actaaatata gattccaacg gagagacaat ttccctacgc agggatattt
    86221 tgttttcaga attaaatcaa atcttagaat cccaaaccct ggaaagagca cagtattacc
    86281 ttaaacgtct aaaaaatggg ataaaagaag aaaaaacaag taagataaat gatattaatc
    86341 ttcacagatg gaaagagtat gacgatattt taaccgacag tttatggtta tttgataaaa
    86401 gaaacacctc tggtgcacat ttaggatggt attggggtaa ttttattcct caaattccac
    86461 atcagatgat gttaagatat acaaagaaag acgactgggt gttagatact ttcttgggaa
    86521 gcggaacaac attaattgaa agtaagagat tgggaagaaa tggaatagga atagaattaa
    86581 atgctgaagt agcaaataaa gccgaaaaac taatccaaat ggaagctaat aaatatgatg
    86641 tgataagcaa tgttattatt ggggatagca gaactataga cctgaaacca actttacaaa
    86701 aatacaatat caataaattt caactgctta ttatgcatcc accctatcat gatattatcc
    86761 ggttttctaa agacgataaa aatgatcttt ctaatacgga aaccacagag gaatttttag
    86821 aaatgtttaa ggcagttgta aataatgtaa catcttatct ggaaaaaggg cgctatttag
    86881 ttttggttat tggagataaa tattccaaag gtgaatggat tcctttaggt ttttattgta
    86941 tgcaggaggt cttaaatagt ggttatctgt taaaaagtat cattgtgaaa aattttgagg
    87001 aaaccagagc taaaagaaat caacaggagc tttggcgtta tagagcactg gtcggcggtt
    87061 tttatgtttt taagcatgaa tatattatgc tttttaagaa aaaatagata ggagaagtat
    87121 atgcctttat atagtaaaaa agagatattg aatttgaaac ttaatagtat taaaagtaag
    87181 gagttaagag tattagccga gaatcttggt atttccacga aaggttcagc ggcggaaatc
    87241 attaaaagga ttttggaaat gaaaccacaa ccccctgaaa atattgttga tgtttacatt
    87301 aaatccattt tccggcaaag catacaagaa cgcaaagaac ttatatctga tgatgattta
    87361 aaaaatgaac tttctaaagt aaaatctttt tcctggggca ccaaacaggg annnnnnnnn
    87421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    87481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngattgtaga tttttaaggt tgctctgctg
    87541 ttttttgcca gagtgaaact gagagttgtt tccggattaa aaggattggg gtaattttta
    87601 tacagcaaag taagcggcgt cagatcatta ttgaggtcgg aattttctgt ggcagttccg
    87661 catacaatca taatattatc cagtgtccaa cccggatcgg ttaagtcaat atcattggaa
    87721 gaatcggtta agcggaaacg aagataaatt gattgtccgg caaaatcact taaagaaaca
    87781 aattcctttc taaagctatc ccactgtcca gatttgagcc aaatttcctg ccagttgttt
    87841 ccatccgggg aaatttccac tctgcagggg tcataattcc attctgtata aaggtgggaa
    87901 tcgaaaataa gccaggtttg attcgtagcg ggaatcagaa tcgggttgat ggttttaatc
    87961 cagacattgc aattaatagc ataaaaacca taaccaccaa tgctgtcggt aattgcatat
    88021 cccgaagcgg caatctgatt ttgcactttc cagggtcctt ctatttccca accggaactt
    88081 ccattttccc aatcctcact gaaaacaatt gtggcaggac tgaggttaaa ctccaaatga
    88141 ttatgacctt ctgtaagatt tacagttccg atatagggag tgtaacctaa agcataaatt
    88201 tgaattggat agtccccggc ataagtattc aagttagcaa aaccattcac aaaaagtgtg
    88261 tcagggaaaa tatcaccgat aataactctc gctgaaacgg gttcagaacc gcaacgaaca
    88321 cctattctta aagtagctgg aggttttggt tccaaattta tctggatatt tgtccacgag
    88381 ccgttattta ctatttgatt attgataatc ttgtcgtaat accctttttt gcgagctata
    88441 acggtgtaag aaccactagc taaaggacgg taaaattttc cattttgggg aaaagaagtt
    88501 ctgggacgaa accaaggcgc attatgttgc tggataataa tttccgcttc tgttggtatt
    88561 cctgctgcag taacggttcc ggtaagcatt gaagaagaag gaactgctga actaaacatt
    88621 aaagctctgt ttatcaacca gcggattcca ttactgcaac gctgaactgt tcccaccata
    88681 acagttgaat ccggttgaat atcactggtt ccacattcaa ctaaaagagc gattgtgcca
    88741 tattgctgat aaagccaatc gtgaaatgca ccctgacgac tcaaattggg atatgcctca
    88801 taagttccag tgccactttg tttgataatt tgagaagcaa aaccggtagc aatagaagca
    88861 gcaaaattaa ggtcgggact gggacgaata tatttccagt taaaaggata atagaccttt
    88921 tccgagaaat tgcccgtccg ggaagaatgc caacaaacgg cataaatgaa ttttctttgc
    88981 tcacaaagct gttgaaagcc aataagttca ctttcactca ttggggcagg accgcgataa
    89041 taatcatagg tctcatctcc aacaggacaa taaagtgtat ccccatgcac ccaattgaag
    89101 gcaaaattgc gatttatatc cactccgtct atatcacctc cggttaccgg atcaaaatca
    89161 aaaataccgt tgttattgtt atcccgttta ttcttacgat aggaaacatc cagattttcc
    89221 gtaactacat tgtgcccttc aggattgaga gttggaataa accataagtc aagctgcgaa
    89281 atccattgag cataaggttg ctgatagcgg taagttaata tttcggcaat attagccata
    89341 gttgtttgaa ctcccaaaac ctcttccgca tgaacttgtc cgacaaatag caaagccggt
    89401 tcgtattcat caatttccac attatcggaa atacgaatag cataaatagg aatatgatct
    89461 tgctgcgaat aaccaatgat atatatttta gcaatatcgg gatgtgactg ctgatgactt
    89521 tgcaataaag cataaatttg ggcataagta taataacaag taggcagggt tgcacttatc
    89581 aatgccggca aaaatagcat agtgcataat attatcttca tcgctatctt cattgtaact
    89641 tcctttgtgt agctggagct cgctgtaatt cctttttttc ttacagagga atgttgtcct
    89701 ccagctacac agttataaag caattttcag tccaaacaac tgttctttga gttaaaatcg
    89761 gttttattta tgctgccaat ctctttttat taaacaagat atctttgaac tggtctatag
    89821 ttatcaattg tttccagccg attttgactc aaagaaagaa nnnnnnnnnn nnnnnnnnnn
    89881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    89941 nnnnnnnnnn nnnnnnnnnn tgaactggtc tatagttatc aattgtttcc agccgatttt
    90001 gactcaaaga aagaagtaat ccggttatct gctttctatt tttcagttag ttaaaaaaca
    90061 aaatcactta cctttttgaa atttatcggg aacgaaaatc aatccaggtt tcacctttcc
    90121 actttttcac ttttttgtat tgacataatt cagcgagcta atttttaggt ttacttgtat
    90181 tgatttataa acaaggagaa ttagataaat gaagaaagga attcatccca aatatgaact
    90241 ggtaacagtt acctgtgctt gtggaaatac atttcaaacc cggagtacaa aagataaatt
    90301 gcaggtagaa atttgcaatg tatgccatcc cttttacaca ggaaaacaga agatagttga
    90361 tactgccgga agagtagaga aattcaacaa aaaatataac cttaaacaag agaattaggg
    90421 ttttacaccc gatatacgcc attttgtccg tttcctgaat acgggaaaat ggcgtttttt
    90481 ctatcttagg gagaagaatg caggaaaaga aagaaatcaa tgttggcggt caagccgtta
    90541 ttgaaggcgt gatgatgcgc ggtccggata aattagcaac agctattcgc agaaaagatg
    90601 gcagtattga attattaaaa actcctttta tcagcatcac acagaagaag aaatttttgg
    90661 gtctgcctat aattcgtggt tttgtttcgc tgattgagat gatgataatt ggcataaaga
    90721 ccctcacctt ttctgccaat cgttttgaac ttgatttact ggtagaagag gaaagcaaag
    90781 gcaaaaaaac aaaaaaaaga agtaaaacgg cagaaaatac aacaaacata gttagttata
    90841 taattgcctt cggtttagct tttctgcttt ttggctggct gccatataaa cttgcggatt
    90901 ggatgcatct ttctcgcaaa gatattctgt ttaatctttt tgccggagct attagaattg
    90961 tcttttttgt gctctatgta tatctgattt ctctaatgaa agatatcaag cggcttttcc
    91021 gttatcatgg tgctgaacat aaaaatgtca atgcttatga acataattgt cccttgataa
    91081 ttgaaaaaat acaggactgg tcaacaatac atcctcgctg tggcacaagt ttcatctttt
    91141 ttgtgctctt gatcagtatt cttatttttt ctcttgtaga tacattggtt tcagcgtaca
    91201 ttttacatac tcctgttccg gtttatttgc gcttggcata tcatattttg cttttacctc
    91261 ttgtttcagg tgtatcttat gaagtgctga aatattccgg taagaattta aaccacccta
    91321 ttgtaaaact tttaactgtt cccggaatgg ctttacaaca tataactact cagccacccg
    91381 ataatgatat gattgaaatt ggtttggtag ctatgaaagc tgctttggat atggatttaa
    91441 gtgaacataa ggtaaaaatt atagaggaat aaatctcttg cttccccaag aaaaattaca
    91501 aatcctggaa aatgaactga aggaaatgca ggaaaaaata agcgatcctg ctgttattaa
    91561 cgatccccaa aaatatcgca actgcctgcg ccgctttaag gaattaaggg aaattaatgc
    91621 tgcctggaaa cattatcaaa ccctaaaaaa gcatattgaa gaagttaatg ccctggaaaa
    91681 aacggaaaat gatccagaaa tgcttgattt gataaaagat gaagagcgtt ctttgcaaca
    91741 acagctggaa cagacagaac tgaaactaaa ggatttgctt attcctgctg accctaatga
    91801 cgctaaaaat gccatagtag aaattcgtgc cggaacaggt ggcgaagaag cagctctttt
    91861 tgctgccgat ttattcagaa tgtatagtta ctatgcagaa atgaagggtt ggaaattgga
    91921 agtaatttct atgaaccaaa cggaactcgg tggctataaa gaaatcattt ttcagctaag
    91981 cggtaaagat gtatattctt atatgcgctt tgaaagcggt gtccatagag tgcaaagaat
    92041 tcctgtaacc gaatcctcag gcagaattca tacttctgct gttactgttg ctgttttgcc
    92101 tgaagcggaa gatatagata tagacattca ggaaagtgat ttacgcatag atgtttacag
    92161 aagtactgga catggcggtc aaagtgtaaa tacaaccgat tccgctgtta gaattaccca
    92221 tttacctaca ggaattgttg ttacctgcca ggacgaaaaa tcccaaatta aaaacaaagc
    92281 caaagcactg aaggttttac gcagtcgctt attggatgag gaaatcagcc gccaggaaca
    92341 ggaaattgcc aaagcaagaa aggcacaggt ctctacgggt gatcgcagtg ctaaaatcag
    92401 aacttacaat tttccccaat caagagttac agaccataga ataaacttga caagttataa
    92461 tctggattcc tttttggcag gaaacataga ggagtttatt caagcattac gacttgcctg
    92521 gagaaacgaa aaggtgaaca cataaatgtc tgtggttcag attgttctga tcctgttggt
    92581 gttacttttc tttttattgc tcagcttttt atttgccggt ttggaaacag ggatgctttc
    92641 tatagaccag ataaaattag agcaggaagc gaaaaaaagt aaaagtaaag cagccattct
    92701 gcagtttgtc cgtcaaccgg atagattttt agggacaatt ctaatcggca ataacatttc
    92761 caatgtgatt atggcatcgc tttccaccta tttagttaat cgtctttttg ctcccgtttt
    92821 tgatgcgcgt tacacttctc tggtggtggg catagttgtg ttgatttttg gtgaaattgc
    92881 tcctaaagca atttttcggg ataatgccga aacccttgtt cccctattat ttcccttttt
    92941 gcaattcttc tattatttgc ttaagccatt tgtaatggtt gttacctggt tgaataactt
    93001 tctgcgtaaa ttgttgaaac tggaagaggg ttatcaatat gactatttaa ctaaggatga
    93061 cctttccttt ttgctttctc aggcagctaa tgccgaaacg gaagataacc aaatggaaat
    93121 gattgaagac gctttggatt ttaccgaact ggatgcagga aatgttatgg ttccgcgcac
    93181 ggaaattgta gctattcaag aaaatactcc tctttcggaa gttatcaata ttgtaaggaa
    93241 agaaggtttt acccgctatc ctgtttatcg gaataatatt gatgacataa tagggatttt
    93301 aattatcttt gatatcttga aaaaggattg cactccggat atgattgctg gagatattat
    93361 tcacgaaccc ctttttgttc ctgaaaatat ggatttggat gttcttttaa aagaaatgca
    93421 aactaagcaa cgcagtatgg ctattgtagt tgattctttt gggggaacgg caggaattgt
    93481 aacgatggaa gatattttag aagaaattgt aggtgatata gaagatgagt atgatataga
    93541 tgaagatgaa cattctgtcc agcaagttgg tcccaatacc tggctggtag ctgctgatac
    93601 ggaaattgat agattagcgg atgattatgg cattgaatta cctgaaggtg actatgaaac
    93661 aatagcaggt ttgattttag accgcctgga aagaattcct catcagggtc aggttattaa
    93721 tattccaccc tataaaatac aaattttgca ggcaactgca aagcagatta ttaaggttaa
    93781 aatacataaa acgagtaaga tagaggttta atatgaaact aatggaatgt gttcccaatt
    93841 tcagtgaagg aagagacaag acaattctgg atgccattgc aactgccatt aaaaatgtga
    93901 aaggggttgc tttactggat attgatcctg gagctgatac taatagaacc gtttttacaa
    93961 tggctggaga acctgaagca gttgtagaag cagcttttca ggcaataaaa aaagcagcgg
    94021 aactaattga tatgagtaaa cataaaggag ctcatcccag aatgggggct acggatgttt
    94081 gtccctttat tccaatttcg gaaatgacta tggatgaatg cgttgaatat gcccaacaat
    94141 taggaaaaag agttggcgaa gaacttggca tacctgttta cctatatgaa tatgcagcta
    94201 ccaaagagga atggagaaac ctgtctaata tccgtaacgg tgaatacgaa gcattaccgg
    94261 aaaaggcaaa agacccttat tggaaacctg atttcggtcc tcatactttt aatgccaaaa
    94321 gtggagctac tgctattgga gccagagaat ttctcattgc ctacaatata aacctcaata
    94381 cccgtgataa aaagaaggca agtgaaatag cacaaataat tcgtgaaagc ggaagacccg
    94441 ccagagatga aaacggtaaa ttgctgaaag atgaaaacgg taacccaatt tatattccgg
    94501 gtttattttc tcattgtaaa gcagtgggtt ggtttattga aagttatgac cgcgctcaaa
    94561 taagcataaa cctgacaaat tataaaatta cccctcctca tctggttttg gaaaaagtgc
    94621 gggaactggc atctgaaatg ggtatgcagg ttaccggaag cgaacttgta ggtttaattc
    94681 ccaaatccgc tttagtggaa gccgggaagt tttacttaca acgcttacag gaaagcaccg
    94741 gcattccgga aaaaatgatt atgcaaacag ctattcaatc aatgggactg gcagaactgg
    94801 ctccctttga tctaaataaa aaggttattg aatatgccat tgccggaaaa gacctgctgg
    94861 taaacctgaa agtagatgcc ttttgtgatc tgctttctac agatgcacct gctcctggag
    94921 gaggaagtgt ttctgctctt tgttctgcta taagtggagc tctttctgca atggtagcta
    94981 acctcacaat cggcaaaaag ggctatgaaa aggtaactga agaagcaatg aaattagctc
    95041 caagaggaca ggaaatcaaa caaaagtcac tggaagttat tgataaagat actgatgcct
    95101 tttttgcttt gatggacgca atgcgtttac cgaaaaaaac agaagaggaa attgcatatc
    95161 ggaaagcgca gattgaaaat tgtactcaac aggcaattaa tgcacctctg gaaacaatgc
    95221 gattggcatt ttccgcttta gagcttgcct ccaaaatagc aaaaataggc aatactaatg
    95281 ctctttccga tgcaggagtt gctgctttaa cagctttctg tgcatctcaa tctgcctact
    95341 ataatatttt aattaatatc cccggaatta cggatgagga gtttaaacaa aaaacccttg
    95401 ccgaagcaaa agaactgctg gaaaaatgta agattattgc ctatagtgta gaaaaatatg
    95461 tttgtgaaca gttaaaaggt aaagggtgaa caggttaaca gtttaacagg tgagacccac
    95521 gataggcata gtgaccaatt aagcatttgt gttataacta tatttttttc cttcttgaga
    95581 aagcaggaat caagataaaa ctcagcaata aagaaggaag aagaatatct gaatgcctat
    95641 tttctccata attttagtcc gtttttttca ggacaactaa accctcacat tcttcatttt
    95701 tcattcgtca ttagtaatgg cttgcttgta tatctgtgat acctgttgca gaaattcagt
    95761 tttattggca taacgcataa cacttctagc tgaaggagaa ggtaaaatcc ctatttttat
    95821 ttcccggtgg ggattatttt tcaggataag ggaattgcca ttatctctta tttcaattaa
    95881 gtcataatgt ttctgctgtt tcagataatc cataagccaa tagtgaactc tatttttgct
    95941 ggtgcataag atttttgtaa gagaagaatt attataaaga attcctaaga tatcctgata
    96001 ttccagaacc gttaatttat agtcctctgc gctggttcct tttctgctaa actttaatag
    96061 gatatctgcc attccgagtt gatttcgggc taaaaagctc tgtagaaaat caacctgata
    96121 agctgtttta atttttctgt cttcttccaa tgacctcaag taagagatgc tatctatttt
    96181 atccggttca aatgcggtaa ataatatttc ccaaagaaga ttactgttac taccgtaata
    96241 gtagtccata tccttatttt tcaggtcatt agcattaccg gtacaaaaac gatagggagg
    96301 tgcactacct aaaagcagaa ctttagcatc ttttggaata aaaggtttat agggatgatt
    96361 ttgcattgtc tccatatcat aaactcctaa aaagtgtagc tggaggacgc tattcctccg
    96421 cttttttgga aaaccctgtt cttatgtttt ttcccatttc caataaacct tgtagttact
    96481 ctattattaa aaaaaattaa attaacgcgg agctacagtg agttctgtct acacaaaaag
    96541 gtgagctatg tttacatttc tgtttcctca tcttcgggca aaggtaaatc aggaagagag
    96601 gcaagtcgtt tttttctaat tcgccaggca ataattaaaa tcacaggaat caataaagcc
    96661 aggagggcat ttatactcca aaacagatac tggtagcgca aatgtttaat atatcttgca
    96721 tactccttgt gaaagtcata aaaagtagta tgaaaagcgc ggttaaagca gtcggtaaaa
    96781 ggtgcctgca gattttttct atgctgttga gctaccattt cccaaaagga attccactgt
    96841 tcatttttct tatttttcag atagctgatt gccattgtgg aagatagata gaatagggac
    96901 caatcctctt ttttttccgg gtagctgtaa ctaagccgaa agaggtcgct tttttcccct
    96961 aaaaagctct gctgcaggaa gtataaatac tgttcaaaac ccatttgccg ggaaaaatga
    97021 gttgccattc cttcgtgaaa ccacaaaggt gtttgcgtaa agacctgttc cagataccag
    97081 tgaatatatt catgcattag tattttgcgg tgattttcct gaatggaagc aagaggtttt
    97141 acataaattc ttttttcggt tccgctatag aaggcatcgc tgaattccac aatttttgcc
    97201 ttgcccaaag ccaatgtttg ataacttcta ttattttcaa tcagataaac cggagcaagg
    97261 gcatccacat aaacgcccag ttccatctga aaagaatcaa tattctcatc cattgctttc
    97321 agcatttctg ccagaggttg ggaaactgga ttttgagcat aaattgccaa gttattggtt
    97381 ttaagctgta aaggatagct ggaaaaaaga aatcgtgata ggcataaaag aataaacagc
    97441 aaaaaatgcc ttttcaagtt ttggttcctt gtttttttaa cagtgaacgg caaattccat
    97501 tttggcacaa ggaattcggc actccgatat cctttaaaag catattctca ggaaaggtct
    97561 tcttggcgta ataattcgta atagcgggta gcgtcctttt tagctacatt tccttccggg
    97621 atttgggtta tccgaatttc gtgttccgca ttatacaagc ggaaaattat aatatctcct
    97681 gcctttacat ctgcagaggc cttagctggt tttccgttaa taagcaccag atttttatcg
    97741 caggcatttt tagctatgga acgggttttt gtcagacaga gtttattcaa aagcacatca
    97801 atgcgcatat ttatccttcc tgcttaaagt tccagagagc tctttccgta aggaaaagct
    97861 cttttaacaa ttcattattt ttcaagaaag taagcgtttt gtctcccgcg caatcatcaa
    97921 ttcttcgtta gtgggtattt tcagaaccgt aaccgaagaa tcatttgcgc ttaaaacttg
    97981 aattccatca gtccgttggg aattttcttc caaatttaat tttataccta tggcttccag
    98041 atcacggcag acctgttcac gcaaaagagg catattttca ccaactccac cagtaaaaac
    98101 caaaacatcc aatccattta agacggcaaa ataactgcca atgtatttct taatgcggta
    98161 acaatagaca tccaatgcca taattgcttt gggattgttt tttactaaaa cctcattttc
    98221 aatttcccgc atatcattgg aaatatgact taaacccaac attccgcttt ttttgttcaa
    98281 aatattgttt acttcgtcca cacttaatcc cagttgttgt tgtaaatgaa tcggaatagc
    98341 agggtcaata tctccgcagc gagtgcccat cattaaacct tccagcgggg taaaacccat
    98401 agaagtatca atggatttgc cgtttttgat ggcagtcatt gaagctccat ttcccaaatg
    98461 acaggtaata atttttaagg attccagagg acgctgtaaa aattctgccg ctttcaaact
    98521 tacatatttg tggcttgtgc catgaaaacc gtagcgccgg attttgtagt ttgtataaag
    98581 ctccaaaggc aaaggataca aatatgccat agcaggcatt gtttgatgaa aagcagtatc
    98641 aaaaacacca cattgaggac aatttggcat atttactttc acggcttcaa tccctttcaa
    98701 attggcagga ttgtgtaaag gagcaaggga aacacattcc tgcatcactt gaataacatg
    98761 ctcggtaact tttactgcat ctgaataatg ttctccggca tgcaccaaac gatgccctac
    98821 agcatcaatt tcatccaaac cgtttaaaac accattggca ggatcaatca gggcttcaag
    98881 aattagttgc aagccctgtt cgtgattatc caccaccatt tcccgtttct ttttggtgta
    98941 tttctcactt ttataggtga atagagcaat atcttcccct attttttcta cgataccttc
    99001 cgccattaag ctctctgttt ccatatcaat aagttgatac tttatagaag agctgccgca
    99061 attcaaaact aaaattctca tttttcctct tttttcccgt ttattttttt tctcaaagtc
    99121 ctgtttatga gctatatgtc aaatagtatt ttctcggttt tcccttccct atcagctaac
    99181 cattttattt acggtaagtt ataaccaata gaagggaaca tttcattctg tatgtttgct
    99241 ccttttcggg taaacgagag ggcattaagt ataattttct acttttttcc gctctttttt
    99301 ccttttcttt aacgctatat agatcaataa gatcccttat ctttcccata tcgcgatatg
    99361 ggaacgatat gggaatcata tcggaaggat atgagtgttg gctaagtgcg aaaagttatc
    99421 ttaatgtgtt tctatatagt tagttctgat cttctgcagg aatggtttgg aatgtaacct
    99481 caaattcctt ttcgggagca aaatcaaaaa attcccagat ataattcctt ccctcttgtt
    99541 tctgcagagg gaaaggaaga gaacttattt gcacacttat gccaggatgc aaagtatatt
    99601 tagcgtaagg tatgggtctg ccccaggaat ttgtagtggt gataatatat ttggcataat
    99661 tgcttttaag ggtttgagta taatcaaggt gcaaagaaca aaaagaaaga gcaggcagag
    99721 aaaggagaaa agtaaaaccg ttcttatctt gatttagcag agttactgct ccttccttgt
    99781 catcttgaac agataatgct ttaattttag gaggcaggca aaaactatca ctgggaacgg
    99841 gaaaaaagat caattcctta gcggaaagcg aatcgtaatt ggcaaaatga aatagaccat
    99901 ccatttccca taaattattt tccagataga aattcagctc ttccgcttca aaagtgagag
    99961 cggaaagcat cataatttca gctaataaca gcaggagaaa agcgtatttc acttaagtat
   100021 aataagttta gctgtcccca atgccttacc ttgatttgta agacgcacaa aataaatacc
   100081 gctggaaagc tttggcagct gccattctat ttcattttgg tcataaactt tttccgcaag
   100141 atggcgtcct ttcaaatcgt ataaaacaag ttctgccttg cctgtaagct tggtatcata
   100201 agaaattcgg atactttcgt tccaatgagg atttaataca gaaggatata gatttagttt
   100261 tcccgcagga atagaaacga tattttcttc cacactaaca ggcaattgca cagaaaaggg
   100321 aataagagct cccggttcag caaaaaactg agtattaaaa gctatttcat tggtaatagg
   100381 attatttacc caaatctgcg ttttcattgg ataaagctcc ccggtagcaa tccctgcact
   100441 attagtaatc gctggattta taaaaatacc tccggcggat tgactattat aaactgaaac
   100501 tccggcaaca ggtaaaccgc ttaaatcggt gcattcaagc ctgtaagtga acttggcaga
   100561 acaataattc ggtccgcaaa cagccggcga aacatctttt gcccaaccat aaacattgtc
   100621 accaataaga ttggaataaa gtatttgtac cgcacattga gttccattta ggggacttaa
   100681 atcgttctcc aaacccgttc cccaatgaac tgtctcttca tatagctgag gaattcggat
   100741 agtaaaaaaa ccacttgcag gtgtattaac actctgtggc acattaattt gctgaacagt
   100801 tacggcattg ggattaacct gaatggtaag tgagtcctcg gcatccagat aaattatttc
   100861 cgctgtctga aatgtaccca caaatgcaga aagctcaatc tccaatacat tttcctgctc
   100921 aaaccaaaat ctgctgatca aatttggcac gatgggattt gaccaagcgg aaatagtggc
   100981 aataaataat attaatgcca agacgatgcg tttcattttt cttacctcct tgagcaaaca
   101041 ctaaaaattt agcacaaata taaataaagc aatttttatt ccctaattaa caaaataagt
   101101 aggggttagc ttgcatagaa cgccaggatt ttatttatcc agaagccact gaaaacatta
   101161 agagcaccga aaataattat aatttgatag tgtttatata atttttatat tcataaaccg
   101221 taaataatag ggataaaatt ccgtatgtat ggcaagaaaa taaggacagg ttttgaacct
   101281 ggtgcaaaaa tgaaaatgat gagtgttaaa aaagggatat cccgtaacta aaagttagtt
   101341 acaagatagc tccttttatc tatggctgat caaagaaaga atatcgggag tcgttactgt
   101401 tgaaaaaact tctataatac ccgcaaaatc tttaggcact gaggaattac ggcaatttgt
   101461 ttataagata gttgcagtcg gcgtcaatta tgaataatac tatattccta ttcataaatt
   101521 ctaaacttaa gcaataattc ctgccaactc taacgggatt gttaatatat agcttttcaa
   101581 aggaaaaatc atattaaggg aaaattatga gtaatgattg cttttataga atagatataa
   101641 tgtatgtttc acctttccat ctttctaacc cttcgccttt cactgttcac tgttcacttt
   101701 ttcgcgtttg acttaagatt ggtaaccagg aatttgacta aaaactccgg ttgataggag
   101761 agttttgcct gtcttaaacc ctccaggcca aggtcttgtt ctcggtttaa atatttatac
   101821 cggttttgga gatattttgc tgtttcccaa ttaattaatt gagcagcacc ttttttctcc
   101881 gggtcaaatt tttcaaaatg ctccgtaacc atatcaggtg tctgaggtga aaaaatagaa
   101941 aaggcagcaa ttttatcgtg caggcaaata a
//
LOCUS       DAOPFL010000002        54546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_518_length_54546_cov_79.289461, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000002 DAOPFL010000000
VERSION     DAOPFL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 54546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54546
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_518_length_54546_cov_79.289461"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLG69_00420"
     CDS             <1..204
                     /locus_tag="PLG69_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPI42001.1"
                     /translation="RCVDVCPMNLVPCMIVSAVKYDNQKLAVRSGLNDCMKCGACAYV
                     CPAQIRLVQYIDTGKIRYAEAKK"
     gene            214..1317
                     /locus_tag="PLG69_00425"
     CDS             214..1317
                     /locus_tag="PLG69_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPI42002.1"
                     /translation="MKDRFIVSPAPHFHDKTTTKNVMWNVVLALTPALIFAIYYWGFN
                     SLLLTLLGAVSAMVTEAIIQKFRKVPITVSDGSAFLTGMLLAYNINAGAPWWVPVLGA
                     AFAIAIGKQVFGGLGNNPVNPALLGRAFLMASWPTIMTAGWAKPKSGTLSGITDPNVI
                     AVNLQNLSPKAYDLVTGATPLKVAQTLRDSSFVSSLGANAGQGFDLANRIFTNLTDLS
                     ALKNLFWGNIGGCIGEVSVFALLLGAAYLLYKNIIEWRIPLFYIGTVFVLSFFLAPLK
                     GSGFSPLLPFFHIFSGGLMLGAFFMATDYTTTPLTKSGRIIFAIGCGILTMVIRLWGG
                     YPEGVSYSILFMNVLTPLIDKLTMPKAFGRVKK"
     gene            1314..1904
                     /locus_tag="PLG69_00430"
     CDS             1314..1904
                     /locus_tag="PLG69_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPI42003.1"
                     /translation="MKIYLQLGLILLAFCVAATALLAYVNSLTKPQIDSIQQKEAEET
                     RRQLIPDSEFEEIKGDFTYYIAKDNKTKEVKGYVFTAEKNGYNGPVKTMAAIDKDFNI
                     IAIKVIQQTETPGLGTNSTQPKFTDQFKGKSVEQLIVDKDGGVPPNCIKAITGATITT
                     RAVTNSLKESITLLKKLVLEKSASTIETQTEEEVHK"
     gene            1901..2491
                     /locus_tag="PLG69_00435"
     CDS             1901..2491
                     /locus_tag="PLG69_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPI42004.1"
                     /translation="MSFMKELTKGIIKENPTFVIVLGMCPTLATSTSVNNAIGMGLAA
                     TFVLICSNIFISLIKNITPDKIRIPVYVIVIAAFVSIVDMCMAAYLPALHKSLGLFIP
                     LIVVNCIIMGRAEAYANKNNVINSIADGIGMGLGFTLALSVVATIREILGNGTWLNMR
                     VMPKTYDPMLITLLAPGAFITLGLLMALMNMLKEKK"
     gene            2491..3093
                     /locus_tag="PLG69_00440"
     CDS             2491..3093
                     /locus_tag="PLG69_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HPI42005.1"
                     /translation="MGFLGQLFTMAISAILIQNFVLSRFLGLCPYLGVSKKMDSAFGM
                     GMAVIFVMTLASAFCYLIYQYILVPFELEYLQTLAFILTIAALVQFVEMFIKKNSPAL
                     YKSLGVYLPLITTNCAVLGVAILNITPLADGTRYNFIWAVLNGFLSGVGFTFVLLAMA
                     GIRERLEMVEMPKSMRGMPSGLILAGTMALAFYGFMGMKF"
     gene            3108..3971
                     /locus_tag="PLG69_00445"
     CDS             3108..3971
                     /locus_tag="PLG69_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPI42006.1"
                     /translation="MLYLIVVPMAATGFSAILLPVAIMGSLGLIFGLILAFASKVFPV
                     TVDERIEKIIEVLPGANCGACGQPGCSGYATAIVNEGKDLNLCAPGGPAVVEKIAQIM
                     GKEATAIDKKIAVIHCSSGGKNNTKWKYAYQGIESCLSAVNIADGPNLCSYGCVGFND
                     CVAACKFDAISIDNEGMRVIDREKCTGCGACVTACPRKLIMLVPASMNVFISCSSKDK
                     NPLPKQNCGADKPCIGCGLCAKKCPAEAITVENNIARIDYSKCTDCGTCATVCPTKAI
                     IDLKAGSRPEA"
     gene            4372..6123
                     /locus_tag="PLG69_00450"
     CDS             4372..6123
                     /locus_tag="PLG69_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42007.1"
                     /translation="MRTFDAVLPYLKKSYTRILGGLVMLILVDLVQLAVPKVMQYAID
                     HIQERTIDQQGLLWIALIIFAFAIAVMVLRFFWRVLIIGNSHSIEKSLRQDFYDHLLT
                     LSQNFFNKSKTGDLMAYATNDLNAVRMLFGMGLISAMDIVLMTVASFAFMISINGKLT
                     LLAVIPMPIISLTVMYFGKKVHTRFKAVQKSFATLSGRVQETISGIRIVKAFCQEKTE
                     LKKIDDVSHDFVNQNIALAKIAGVFHPFMGFIVSISMIITIYFGGKSAIRGEISVGGF
                     IAFFQYLGMLVWPMIAIGWIVDIYQRGTASLKRLNEIFEIKPEIDDVFADERITHLEG
                     KILFQNLSFRYSDNLPLIFKDITAGIEVGKTLAIVGPTGCGKTTLIELLVRIYNPPRN
                     SIYVDDYELYTIPLNVLRRDIVLVPQDIFLFSESIADNIRLGNPDASDEEVFEAARIA
                     QVYDEIMEFEQKFETVVGERGVTLSGGQKQRVAIARALLTNPNILILDDALSAVDTKT
                     ERRILNRLIEIRKGKTTLIIAHRISSLQHSDLIIVINEGIIAEQGTHQNLLAKGGLYK
                     DLYEKQRIRARLEGEEE"
     gene            6123..>6506
                     /locus_tag="PLG69_00455"
     CDS             6123..>6506
                     /locus_tag="PLG69_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42008.1"
                     /translation="MHGGGGGGGPMGQGAYFEDELKTKIYNKRLFSNMLRYLKPYLKW
                     VILSFLFLMLISGAEVIIPLIQRSAIDDHIVSDKSIAVFTGENDYQNFIKRYEKQGFK
                     EYKYGENHFVIINAKDRNKINRTDLE"
     assembly_gap    6507..6606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6607..8446
                     /locus_tag="PLG69_00460"
     CDS             <6607..8446
                     /locus_tag="PLG69_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42009.1"
                     /translation="FVIINAKDRNKINRTDLEYLQEKGILKAETVFLLSDKDENINIL
                     KKYLQENLHPDTPNLSGWYKIGSKTIAVPKKELNKLPKEERLKVRNETVQKLLILALI
                     FLAIITLRFIASYFQTVFTAYFSQNAMADLRHDVFAHLQKMPTKFFDTNPVGRLVTRV
                     TNDIAAIDEMLASGVITLIQDVILIITIVILMLALNWRLALVSFSILPLVIWVIVIFR
                     KKTRVVYREVRKHLAVLNATLAEHIEGQKIIRLFNQYFHKRQEFATINQKYYLASIKQ
                     IRLFAFFRPIIHVSSQIAVALIIWYGGGKILQNVITIGLLMAFTQYISKLFEPINDFS
                     DKFNFLQSALASAERIFNLISLTPDDYRVEMHKNIKMKGEIEFQNVWLAYNDEEWVLK
                     DISFKVAPGEKIALVGHTGSGKTSIVNLILGMYPFQKGRILIDGKDIHNYSLADLRRN
                     VGIVQQDVFLFSGNIKDNIALNNNDLTDEEIIRVAKYVNADKFISQLPRGYYEPVMER
                     GATLSTGQRQLIAFARVLAYNPSIFILDEATSNIDTETELLIQDALNKVIKGRTSIII
                     AHRLSTIQNVDRILVLHKGEIVEEGSHFELLDKQGLYYDLYRLQYT"
     gene            8670..11474
                     /locus_tag="PLG69_00465"
     CDS             8670..11474
                     /locus_tag="PLG69_00465"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI42010.1"
                     /translation="MKKYWLIILALTLVTAIFADGNRYQVIAYGEDFESGAPGWTHYD
                     ATVSPNNWHIYNFGGTQGNVWWMGDPALAQGTNIGGYYNHQYLVLDTPARTLTSANAT
                     LTFKMRHNVETPGSNPPWNGWDACNVRVSTDGGTTWTPISGNPAYDFTSAYSFGFEHG
                     EGQGIPGWGGVREWTNCTFDLSAYVGQSVKIRFAFASDPAYCTSDAPAMYGMMVDDIS
                     FGGYSNNGVDDGQMTYSSLVPVGEDLWHIATDATAPSPTHCMVCQNASGSYNTNMLNY
                     LVSPPITLPNSGDMWVDFMVKGSYNDPNAFPEVDYFGWEISVDGGNTWYAMSNPYGTP
                     GGNNYVYTGLQNTWQSMTEGFGLSFFITEYHGETAIFRWYFKSDGDTPDGVGMFIDDF
                     RVYNDVFIAAPENLAAAVSGNSVTLTWNEPGSGGGGGEEGWIYYCGEPAGNSIGTNGV
                     ADFSVAAKWDAIGETNSIYPYVGMNITKIKFVPAEPTTLCSYAVRIWTGAAGVLVYDQ
                     AVPTITEDVWNEVILTTPYTIPAATTIMVGYRCNAQGGFPAGCDNGPQVEGYGNMIYM
                     NNNWHTLTAESASLTYNWTIQVYVADATGKEYVLGELPMNNSFAEGKFGVHYTEPVRG
                     RDVTGYKVYRDAGYVATVDGNVLTYTDTEVPGGPHSYYVTAMYGTVESGASNTVSVFI
                     LPAEHYEINHDDGTAEQGFSVGATKQMAVKFECNDEYDLKYVKVYVHTLGSAGLVIRV
                     YDANGPNGMPGAQLVTQYQYPAASVVAGWNFITLPTPVHLNNQAFYVALFETSGASLI
                     GLDTSSNGFSYQNTSGSWVPVTTGEIMVRAIIQGGNAGEDLTLPPVVLDARNYPNPFN
                     PETTISFSIPASGQTSVKIYNLKGQLVRTLVNETMNAGYQKVVWNGTDDNQKSVASGI
                     YFYRVNNAGKTITRKMLLAK"
     gene            complement(12172..12924)
                     /gene="phnC"
                     /locus_tag="PLG69_00470"
     CDS             complement(12172..12924)
                     /gene="phnC"
                     /locus_tag="PLG69_00470"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HPI42011.1"
                     /translation="MNPNSNLLQVNNLSKSYDGKKWALEDLSLKVQEGDFVILLGLSG
                     SGKSTFLRCINRLIEPTRGQILFRNEDILQLKGIELKHYRRQIGMVFQQFNLVKNLTV
                     LTNVLTGRLGYHSSLSPFSKEDYELAKQNLEKVGLQDFADKQVKFLSGGQQQRVGIAR
                     ALMQKPSLILADEPVASLDPATADSIMQYLGEINREGISIICSLHFLTLARRYGNKVL
                     ALKEGHKVFEGIPNQIDNIRFKEIYGQDAEEI"
     gene            complement(12921..13847)
                     /locus_tag="PLG69_00475"
     CDS             complement(12921..13847)
                     /locus_tag="PLG69_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPI42012.1"
                     /translation="MCKKYIWLAFVALLIFGCGTEESRLEKANKKLGSKQNPIKMYFV
                     PSLEAGKVVSSGEAIANYLEKETKLHFKVAVPTSYAAVIEALGTYQADVAWLPTYAYI
                     LAKQKYDAQVRLMTVRNGLTKYRGQFVARSDSKINSLQDIEGKIIAYTDAASTSGYIY
                     PSAILKQRGITPKDYFFAGGHPQAILAVYSGRADVGCSYWSPPDAKGKPMDAREKLLE
                     TYPDVFEKVKIVDYTDWIPNDTVTFRKNLPPELEGIIVQALYRYAQSAEGKKVLKALY
                     DIDGLEYASDKDYEIVRTTLKTMNMDPAELLK"
     gene            complement(13917..15188)
                     /locus_tag="PLG69_00480"
     CDS             complement(13917..15188)
                     /locus_tag="PLG69_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HPI42013.1"
                     /translation="MAILIQNVLPRSLAAKNDIKSGEKLLSVNGQPVNDFLDLEFYTS
                     DYEFELEIMSAEGISRKVKIQREEKSFLGIEPEPYKIRYCENACIFCFIDQMPPELRP
                     TLYIKDDDYLYSYVFGNYITLTNLDETDYARIINQRITPLYVSLHTTDNALRQKIMRP
                     SKQVDALSVLKKLSRHGIGFHIQIVCIPGINDGEALKQTLKDLHDSEINCLSIGIVPV
                     GLTKYRKNLDAIIPYNRQTATEILALIDEARKEYNSTIIYPADEFYILAERDIPDEDF
                     YADYPQLENGIGMLRLTIQTYKQKKRGLLKELRKKPVNYLMISSVCAQNVITRIAEDL
                     NKRLENQFIRVQVIHNDFFGPDISVCGLITYSDLWQQLAPQAGETIILPASIFNNEGE
                     TLDGKDHLTFQETWKNPILLIDQFFEDWDYL"
     gene            complement(15210..16331)
                     /locus_tag="PLG69_00485"
     CDS             complement(15210..16331)
                     /locus_tag="PLG69_00485"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPI42014.1"
                     /translation="MKKKFFVFFALLTCLGMLLGNITIQIKGEAKPVNLQETTLSSVS
                     YVALKDFSAIFKAISKEDRSDNRLYLNLYDEQFIFLENSPYYTLKTVSYNMHYPLLRK
                     GESLYLPSVFVTEQLKTHFPSLIQRKGSTLQIAKPIDNSVKTIVLDPGHGGKDPGAIG
                     KKLKANEKDINLAVALKLKNLLEKELGVNVLLTREDDRFVSLYDRTRFANEKRADLFI
                     SLHSNSSKSTTSRGIETYYLSTAQTSDARAVEAMENAVVERFEGGSEAKKKYDDLSFI
                     LSDLAQTEHLENSNNMAFNVQQNLIAGTQSIDRGVKQANFYVLRGAFMPSILVEMGFI
                     SHPEEEQLLVNEEYQDRLARTIFEGIKRFKFHYDRIRNT"
     gene            complement(16328..17272)
                     /locus_tag="PLG69_00490"
     CDS             complement(16328..17272)
                     /locus_tag="PLG69_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPI42015.1"
                     /translation="MSYSQMFSCNICPRNCGIERAKNKGYCGVTDKLRINLATLHFGE
                     EPCFSGTRGSGTIFFAGCNLRCVYCQNYEISTLCWGKDISVEELIRLMLKLQEEGAHN
                     INLVTPTHFTLQLREAIIQAKEKGLTIPVLWNSSAYEKVETLQLLTGLVDIYLPDFKY
                     AHKVYAQKYSSAPDYPAVAISAIKEMFSQVGLLELDEKGIAQKGLLLRMLVLPNKLAG
                     CKENLYTLTEELGTELTLSLMGQYYPAGKAKNYKELTRGITLSEYFEVVDTAVELGFT
                     KLYTQEISSSDTWTPNFSPAPEEINPVSEFNPQLELTL"
     gene            complement(17273..17674)
                     /gene="cdd"
                     /locus_tag="PLG69_00495"
     CDS             complement(17273..17674)
                     /gene="cdd"
                     /locus_tag="PLG69_00495"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPI42016.1"
                     /translation="MVELNLSEKELLEAAKKAAENSYSPYSHYKVGCAVKTKDGHIFT
                     GCNVENASYSLTICAERNAIFKAISEGHRTFSEMAVYVDSEESFPPCGACRQVIYEFA
                     PEIEIIYANRKAIHKALITELFPSAFTLKKD"
     gene            complement(17688..18788)
                     /locus_tag="PLG69_00500"
     CDS             complement(17688..18788)
                     /locus_tag="PLG69_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPI42017.1"
                     /translation="METLFSGLLGFQLKQVIMIIIGLILIWLAIKHKYEPTLLLPIGF
                     GTILANIPFSAAVGEHSPLGILFKAGIDTELFPLLIFIAIGAMIDFTPLLKNPFMLLF
                     GAAAQLGIFVTIVLAAGLGFEIKEAASIGIIGAADGPTSIYVANRFAPNLLGAISVAA
                     YSYMALVPIIQPPVIRLLTTKKERLIRMDYHSGDVPQIVKIMFPIAVTIIAGIFVPAA
                     LALIGFLMFGNLIRESGVLEGLSKTAQNELGNLVTILLGLTIASKMQGELFLVPQTLL
                     ILALGLVAFIFDTAGGVLFAKLLNVFKKEKINPMIGACGISAFPMSARVIHQMGLKED
                     PFNFLLMPAVSVNVGGQIGSVIAGGLILALLA"
     gene            19096..19656
                     /locus_tag="PLG69_00505"
     CDS             19096..19656
                     /locus_tag="PLG69_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42018.1"
                     /translation="MRNIVLILLVALLLTGCDLFSVRDSEPPAAQIPWIDFTTEVDSV
                     LLNLEYSYEYPQNAVNYARLFSSDYVFHFAPQDVSDFSIEQTWTRVQEQDMIQVLHNR
                     YKNMQVTLEKVDDQDQIESAQAKIYRQYNITGISRNEGKEVLTLAEGNMELHLKKEYG
                     YWYITKWYDYRSSNVPTWGKLKYENH"
     gene            19643..20218
                     /locus_tag="PLG69_00510"
     CDS             19643..20218
                     /locus_tag="PLG69_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42019.1"
                     /translation="MRTISIILLFLTLLIGSCNNPFCPELYDVSNEEIQNRTPEELLQ
                     NLERAYTERNINIYKSLLHPDFRFELLASEVSLIGIDMNNDGMRDSWWGYAQEVEYTD
                     RMFNKGSSDGTYPPPDDIKLRLQIPPSERWENDPEVGHEDWIIIPCNFDLILSYQESN
                     SSITANGIARFYLRPVGNGWKIAIWRDESNI"
     gene            20215..20631
                     /gene="ruvX"
                     /locus_tag="PLG69_00515"
     CDS             20215..20631
                     /gene="ruvX"
                     /locus_tag="PLG69_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPI42020.1"
                     /translation="MSSKGRILALDYGEKRIGIALSDPNRIFSKPLCVLSNKGFEQLV
                     EELKKLIALHQVTLLIIGIPYAIDGSITPKTEECQQIMNRLKDVLSIPVEGFDERYST
                     WEANEELKKMGYNWRQAKEIKDAMSAAMILKEFLNQ"
     gene            20628..21611
                     /gene="mltG"
                     /locus_tag="PLG69_00520"
     CDS             20628..21611
                     /gene="mltG"
                     /locus_tag="PLG69_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPI42021.1"
                     /translation="MKKAKPFLIGLAILLCLLILVIVYLVFVPLHSEERIVRIKQGDN
                     ARIIASKLSEAGIIRSKTMFIILTKIRKADRNLKPGSYIFGGNTYLWQTVSRLYKGQN
                     ESITITFPEGLSLYKTLKKIDASGLATYEELHKAATNPFLVKKLTGFDALSLEGFLYP
                     ETYRFPIEISPDSILAIPAGEFFRRLQKEGIDPFAVPDFYDKLILASIVEKEAGDESE
                     KEIIAGLFLNRMRQQMALQSCPTIDYILEPQGIKRSVLTYADTQINSPYNTYLYQGLP
                     PTPICNPSITTIKAVLNAKPNSYLYFFSDRQGKNVFSKTYEEHLAKQRTML"
     gene            21888..22709
                     /locus_tag="PLG69_00525"
     CDS             21888..22709
                     /locus_tag="PLG69_00525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPI42022.1"
                     /translation="MLGPYEINDIYQTDCVEGLKKLPENCIDLCVSSPPYDGIRDYHG
                     FSLDLHNVGIELYRVMKEGGIAVMVMQDQTRNFAKSLTTFRTAIDWCDNAGFRLFETL
                     IYRKYGAEGGWWKTRFRVDHEFMLVFLKGQRPQYFDKEPLKIPSKHGGKTLTGGGTRL
                     TNGIRIPTRPILINEMKCRGTVWEYLTAGDGSRLKHKHPATFPDKLPYDFIQCFCPPE
                     GIVLDIFMGSGTTALAAIELNRNFLGFEISEEYVKLAKQRIAIEGKKDEDQLMFL"
     gene            complement(22732..23706)
                     /locus_tag="PLG69_00530"
     CDS             complement(22732..23706)
                     /locus_tag="PLG69_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42023.1"
                     /translation="MENNLDVLNYQELIKKYSWILERDHNCILSPDSDGLLCGLFMSN
                     YLNWKIVGFYDGKILIKDKKIDLNECIFLDMEIFRDFIRSAGHHIVLYSQRAIPELWT
                     NLNQCIQPNLLRGYYGQTHFKNKYPLAMIHLLIGILDNQEKINIETESICPLLFTDGT
                     FKNLFNYPENCLSWLHYLGADRKSSALHKIFFNECYTITSLMIALKELFKVISQDDYS
                     DKIKISTREGKIDGLQKDNSYFRFDDNTWLKTENFLKYLSAKTKWNYIQDKWTKSDFD
                     VFQFTKKSNKARVGIFRQILSENPLSMAQTSGNLIEYTIDPHNIFKNI"
     gene            23940..24506
                     /locus_tag="PLG69_00535"
     CDS             23940..24506
                     /locus_tag="PLG69_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPI42024.1"
                     /translation="MKLNKMMLLLVLVAVVVMIASCSSNKKANIKPQGEEVYRPAWWA
                     TQPEQDYVCTYGQATKVSENLSVEAARANALQEAAQYVEVLVNSMLKNYEEEAGVKDP
                     QVLALTQNVVKAVSSAKFTGVLMGKIETRKVNEPDGVRYKTWIQLKIPKDEINRKLAN
                     SIRNEEALYNQFKASQAFQELDKAVEKY"
     gene            25030..26586
                     /locus_tag="PLG69_00540"
     CDS             25030..26586
                     /locus_tag="PLG69_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HPI42025.1"
                     /translation="MLKLGLNQEMVSRARKAAFQITNAFTWLFEGYTTVATERTVLRL
                     MGIDGALKDGKPLPNVVVDHLVENSALQKGAAIWVANAMLKKGLTAQQVAEEVAYKGL
                     DITQIPLASGEAILETLNPLVDKMLGHITAQKQKREELLAKYQPRPQPVIYVIVATGN
                     IYEDVVQAKAAAREGADAIAVIRSTAQSLLDYVPFGATTVGFGGTFATQENFKIMRKA
                     LDEVAEEENRYIYLTNYASGLCMPEIAAMAAMERLDMMLNDAMYGILFRDINPKRTLL
                     DQYFARMINAYAGIEIQTGEDNYLTTSDAVEKAYTVTASQLINESFALMSGIKPEKMG
                     LGHAFEIDPELENSFSFELAHALLTKTLFPDAPVKYMPPTKFASGNIFKTYLLNAMFN
                     FIGQLTGQGVQLLGMMTEAIHTPHLADRFLAIENAKYIFKAVKDLNQNLCLSEDSFIN
                     KRANEVLAQATEFLEKVAREGLFSAMAKGEFADIKRPENGGKGLEGVIMKDKDYYNPF
                     LEIMKQELAL"
     gene            26583..27326
                     /locus_tag="PLG69_00545"
     CDS             26583..27326
                     /locus_tag="PLG69_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HPI42026.1"
                     /translation="MSKNIIRPYGDTLNDGKMQLSFTLPVSCDALGKEAARKLLLQTG
                     FDEAEITSMSDLTEGFTHFIAYAVTSTSVDISKIKVKVVETPVMTMEETDAFVAEHLG
                     RKITVVGACIESDAHTVGIDAIMNMKGYNHRYGLERYHSFNAINMGAQVPCEELLAKA
                     REVNADAILVSQVVTQKNIHIKNLTRLIELAEAEGLRDKMLFICGGPRISHELAIELG
                     YDAGFGQGTYATDVASYIAITLAKQKAQE"
     gene            27358..28656
                     /gene="rseP"
                     /locus_tag="PLG69_00550"
     CDS             27358..28656
                     /gene="rseP"
                     /locus_tag="PLG69_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPI42027.1"
                     /translation="MLTLLVTIIAFGLMIFVHELGHFLMARSFKVGIERFSIGFGKAI
                     WTTEKNGIQYRLGWIPLGGYVKMQGENPEEEISVDKENTFLGKPWWKRALIAFSGPFA
                     NLLFGLLLFIIAFMLPQKQEDLVPVIQNAKGIWAEAFSPADSIISVNGKPIKGFQEFL
                     VSLSEKKPNTISYFHNGQKTVLEVAPSQVDSLIKSLEPKVDTTIGEVFTGMPAWRAGL
                     KPGDKVLAVDSVNVSNWYEMREKIVGSKNDEVLLTILRDGKILQRKIALEENVSMGDQ
                     KMIGISQYMPVKSVNRYNPLQAISYGTQSTISFIVMNYVGLYKLISKPEQLKNNLGGP
                     VMIATMGQQVAQRGFSSLIIFLASISLILMIMNLLPIPVLDGGHIFFAFLEGIFGKPV
                     PIKVQAFLQRVGFAILLLLMFYAFYADISKLLIRQFLLGQ"
     gene            28657..29769
                     /gene="tgt"
                     /locus_tag="PLG69_00555"
     CDS             28657..29769
                     /gene="tgt"
                     /locus_tag="PLG69_00555"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPI42028.1"
                     /translation="MFNFTLQKTNGKARAGIMKTEHGEILTPVFMPVGTLGTVKAMSP
                     KELEEVQAQIILGNTYHLYIRPGHTLIQKAGGLHQFISWNHPILTDSGGFQVMSLAAL
                     RKISKEGVKFQSHIDGSPHIFTPEKVIEIQEALGADIIMSFDECPPYPSTRKYVEKSL
                     ATTLDWAERGKKAFTNYKHQALFGIVQGGIYEDLREKSALELMEMDFDGYGIGGLAVG
                     EEKEDLLRITSFLNDILPIDKPRYLMGVGTPSDLLNNIDRGIDMFDCVMPTRNARKGS
                     IFTRYGKMIIKAARYKDDFSPIDPLCGCYTCTHFSRAYIRHLITMNEILGMRLTTIHS
                     LWFYQELMQMARKAIWENRWNEFLAEMLPALDRIVQ"
     gene            29759..31537
                     /locus_tag="PLG69_00560"
     CDS             29759..31537
                     /locus_tag="PLG69_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42029.1"
                     /translation="MFSKKTFLIYLGFLAIILVRGTLFCQETEFTSPADSIKITVPEP
                     WTENWLYKKVYPLIFKMEAELQQEQNLFSIFEQYSGKKIAKIYIVNLDVFEMLPDKPE
                     THIYNRILDLGNSLHYKTRDWIIKDMLFFAEGDDFNPEIMNRNLAYLKDLPYLREAEL
                     LISDNEDGTVDVFVLVRDKFSLELSGRIISQSKYRLKINEQNILGLGLGLKHIWHINP
                     KEMKTLSWETYYSDSNIKNTFFRVDAFWKEFSGNSNQDLYLSHPFLYPATPHSGGIEG
                     TRNYIHPPADTLTTEKWTLGSWYAHSFGFSDNLTNAYKYVAFGLEKNWFTKSPQVDEN
                     TGKPWQDNIFALSSFAFTKTGYTSFRNISYFLVNNVLPVGYLTETLLGYEFGEYHNRL
                     FTGLHYSRADKLPRGSYLYFASALETYISSEGTEQSVLAVEPLFISPLQHIGDFQSRY
                     FFNSNLVLGGKRYPSEQMNVSSLSGYHGIWNLQGDKLLRSNLENDITTPYQIWGFKIG
                     LFAFLDCAFATNHFSQISTKNVLLTEGAGFRIHNPALIWESIELCFALNQKKGSKGEW
                     KITLSTKKGLDLPDFSGKRPQTYKFQ"
     gene            complement(31608..32702)
                     /locus_tag="PLG69_00565"
     CDS             complement(31608..32702)
                     /locus_tag="PLG69_00565"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPI42030.1"
                     /translation="MFYCPTRIIFADNAMQKAKEYIASFGKRALIVTGKNSAKQSGAL
                     SDTISVLREADISYALFDRIAENPTLDIVMEGKSAFLQNNCDFVIGIGGGSPVDSAKA
                     ISLAAANSLNRDEMYNTSVFKKAYSILAIPLTAGTGTEVTQYSVLSDPLTKKKAGFGC
                     DLAFPTLAVLDPKYTLTLPPKVTLHTALDALSHLLEGLYSNQRSPIVYPFIYKGIASI
                     MKFLPIVLSEPENYLAREELMRDALYGGMVIAQGSTTLQHSIGYPLTSFYGIPHGLAN
                     AMVMEDIMELYYPAVQQELDDLFSYLQISKDDFYTWLKSLPFERKIDFSEDFIDKAIP
                     QIMSSRNMALNPLPVTEQQVRTLLENLRKT"
     gene            complement(32710..35634)
                     /locus_tag="PLG69_00570"
     CDS             complement(32710..35634)
                     /locus_tag="PLG69_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPI42031.1"
                     /translation="MRLQVKKVLLTLGLFISIGVLFSQTIENVKPGHKYSSPMPQNAY
                     LEEEICRADSAHGFDVLKYEITLTINDALHYIWGNVLATVIATENLTSMSYELTGFNV
                     SNVLVNGIHTPIINYGTYFTFPVNVSAGEQFTTQVFYSGTPQLVYSYYNIGMIFSNNT
                     VFTISDPDAARAWWPCYDHPWDKAIVDLHITMRSDWKVAANGIRTEIVNNGDGTSTTH
                     WIGENPMTTYLVCITAGPYVEINQTVPEQNNLPVQNFVMQNQYNNALIDLQNLPWMIS
                     WFSELFGDYPFEKYGNAVVSMSTYGAMEHQTMTTLGNYIISGNGAYETVIAHELVHQW
                     FGNAVSFLTFKDVWLSEGFATYGEFLWTDKRFGWQSACNYLHSNFHQFYLNWEANSGP
                     QTIYNPSFYNYFSPPSYEKAASVLHMLRLKLGDTNFFNLLRLYYNTYKNGNAITAEFE
                     ALAEQVSGQDLSQFFNQWIYGSGIPGVKYSLWQNETNNHLEIYAKTTSPTATSFTVEI
                     PFRITQNGISDSLLVIAAPDGYSNDFNYSVLSDNINIVSNYNHWTLLKELAEQIPHFT
                     EYLPTNNCVLLFWNTFIPNKDHQYRVYRKLSSAQNWSLLATLNNDTFSYADTTAISGI
                     SYDYQFRVVDVNGYFSRPSETVSVTPQAFSFTGELLVVDETRDGIGTLINPDDEMVDT
                     FYASALSLFSCPIDNWDCATQGLPNLETLGNYKLVLWHSDDFNQNLLQGNSSLLSSYI
                     AGGGKIVISGWKTPSVLTTEFFNRFGGGVTPIYDNLACLISAQSNIYPTLTVDTNKTL
                     SSWNSMLPYIFTFSGAEEPLYFANMMEGSNGAGQCIAFKHDFNGTLVLFGCPLYFMHL
                     DEVTNMFYELLPTLNPALPNSDETIIVPQATLSAFPNPFNPTATISFSLPVGGKAELV
                     LYNLKGQKVVTLSEGLMPQGKYSIYFNGSDEKGRALASGVYLLRLQHPAGIITKKITL
                     LK"
     gene            complement(36341..37096)
                     /locus_tag="PLG69_00575"
     CDS             complement(36341..37096)
                     /locus_tag="PLG69_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HPI42032.1"
                     /translation="MNKPTIGQRLSLVIKAMRLKDYQFANKYGISRASLSRYKLNERY
                     PDPEFLEALSRDQINLHWLFNGNGSMYAKDEFQELIQSNQVPISSTSSDKSNPAIISP
                     ILHPVDNQDFDPTRSITFPIVGEIAAGPPMEIRDEWSQVGQIQLPVSFLPGSPKQYTV
                     FQVNGQSMEPVIQHQDIVVIKSDQSWENADGKIAVVRTDDGLTIKKVQIDHHRQQIIL
                     QPFNIDYPVLVLDSDWNYGAFLIGTIALQLRFF"
     gene            37339..37911
                     /locus_tag="PLG69_00580"
     CDS             37339..37911
                     /locus_tag="PLG69_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42033.1"
                     /translation="MTTKKRCQSEVQKRANQGNVQAGTPVQAGVGMRLNSLITMMRGQ
                     NVQALDRIFQDFRLKCPSGSQFLKDLDMDIQVDLIWLPIDRVALLLNKNLKTIRRMVD
                     AGKLTALKHQIPTKNGATIKTFVLATEDIIQAEYDRFRDSLRKPELYFEEVIELGVHS
                     YHSVFLVYYENGNDLVAKASDKSGGSDVSL"
     gene            37898..40495
                     /locus_tag="PLG69_00585"
     CDS             37898..40495
                     /locus_tag="PLG69_00585"
                     /inference="COORDINATES: protein motif:HMM:NF020859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HPI42034.1"
                     /translation="MFPYSGRPTEEFLREYRSKLNELQELMESQGLKLQIKDGGRCIV
                     LPEVKSGMGKVSGKSKDDGDGISNSTNTASETDNSFMLSYSQQYNAKAGTSEDNLESE
                     TVYETYDDYMEFERKLALIHEARDNGVVIDFSSDFLDLAPVEEERLLYKDEAQLYGRF
                     CQEVIRRLYGAESKILAWKRITSDYNCKRLVPELYNLKGPRKERALREWLRVYHETNL
                     DMYALIHKSKGKYKERKISYQEQQYLLHLLLNPNRIKVGSAITNLKQMSRNGVIESQT
                     NERTLRRWCEEWAARHPAEWHQARDGSKFVSENVIKSIMRDDSTLQVGQVWVADGHTL
                     SFDILNPQTGRAQRMTMIMVKDWASRYPVGASLAFTEDSQHILTAFRNGFINCAQIAD
                     NNSGTMAVLPKYVYLDNGKAFRAKLFHDRWEEHDLNKELGGIFPRLNIGVRFAESYNA
                     KAKIIERFFKTFQEQFERFITTFRGASIDDKPSVLHRNEVWAKKLYAGKPPTIEETMQ
                     MIAFYVRFCYGETPHTSLNRRTPYEVFSAARIVESRQIEISRLNFLMMAMERKNIRAE
                     GIRLDKKVYWHRELVNHVGQPCIIRFDYSDARWIVVYDKNDVFICQAALRKTQHPFIE
                     VSMDQAVSHKELNKEYKEIKGLRREKERSAKKWVVNSQRAVDALLKNSHAKQEKLEDE
                     GARALFSSPPMLEAPPKDGDEIIEELTRKMMMRLPQHPDMLSPEEREQLDPKEKERLA
                     EQERDRIILKEIKAQFDRIGLEYKPGNDKPSLEFAIGFSQTAREYCKAQQQAQNEQEA
                     SDEDEATTATATTYVPGKEAGDHDPAQTIINPAELPDDEQAQDEAKNGQSPFAHLSRA
                     ELLKRIGIN"
     gene            40508..41218
                     /locus_tag="PLG69_00590"
     CDS             40508..41218
                     /locus_tag="PLG69_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42035.1"
                     /translation="MKQGKLVRTQNVIAADKCIEYLLTRPKLEMVGLGLLYGRPGLGK
                     TTYASRIAFSQGYLYLRLEATTTPKAFASKLLLALYKRFGMGEYIPYGTANDLFMMCI
                     TVLEDHKDTIIVVDEIDYAFRHPQLLGAIRDIVDVTTAIVILVGMQNAKDRLNQINEY
                     YFDRCNFFYEFLDLPKKDIAQICSEVLEIKFQPDIVDYVYFHSCGNVRKAMKLIRSVE
                     EIGRYKNLEVVSQADLDD"
     gene            41226..41729
                     /locus_tag="PLG69_00595"
     CDS             41226..41729
                     /locus_tag="PLG69_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42036.1"
                     /translation="MTDKELILNFINQYDLLFNAELIAKLTSVSREAIEKLLPDLLQS
                     QAIKQIEDSPPIYVRVNRYQARIGYQHYKGWTFSIADAHKLLDILEQGRYKSIRDIAQ
                     AIGKSRQWVYIYLEALASIEVVDLRQHIYVVISRQNVPKIGRKVQKGILGQLRSLNRA
                     GCYRLIE"
     gene            42124..42462
                     /locus_tag="PLG69_00600"
     CDS             42124..42462
                     /locus_tag="PLG69_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPI42037.1"
                     /translation="MDKKIAIPTTNKVLSAHFGHCEKFVIYNVVDSKIVNDEWITPPP
                     HEPGSHPKFLREMGCETIIAGGMGERAQTLFAENNIEVFIGVPDIPLTELVQMYIKSE
                     LKSGANSCDH"
     gene            42475..43338
                     /locus_tag="PLG69_00605"
     CDS             42475..43338
                     /locus_tag="PLG69_00605"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42038.1"
                     /translation="MKLTIASGKGGTGKTTFSTNLAMYLAEQYKVVLTDLDVEEPNSG
                     LFVHGRQTHQQDMFKMIPEWDKNVCTLCGNCQQLCSYHAVMKLGSQVMVFPELCHGCY
                     ACSELCPAHALPMHQIKMGELRQYRAGNLDFIDSRLDVGQEQAVPLIAQTLDYVERNF
                     DTAYLKLYDAPPGTTCPVIKATQDADLVILITEPTPFGLHDLKLAIETMRELEKDFVV
                     VVNRFGIGNDDVLRYCEKEGIIVLAKLPNDRHIAELYSRGELVYPHIPEFAEQLRLIG
                     DFIMSQQGMQS"
     gene            43335..44222
                     /locus_tag="PLG69_00610"
     CDS             43335..44222
                     /locus_tag="PLG69_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42039.1"
                     /translation="MKEIVVISGKGGTGKTSITASFAMLGGKDIVVADCDVDAADMHL
                     LMKPNVKTEEDFYSGNAAVIDRAKCLNCGKCASVCRFDAINLIEYLYSVSALNCEGCG
                     YCARVCPVKVISMVEQNVGKLYISATKADNMMVHARLLSGADNSGKLVARVKKEAKQI
                     AQETGKGFVIVDGSPGIGCPVISSLSGADFVVLVTEPTVSGLHDLKRVYELVNRFQIS
                     AGCIINKADINQDVVNHIEIYLRENNIVHLTDLPYDESFTSAMTIGQTIVEYDHDKLA
                     DLLTVSWNKIKQILSKEQQ"
     gene            44219..44557
                     /locus_tag="PLG69_00615"
     CDS             44219..44557
                     /locus_tag="PLG69_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPI42040.1"
                     /translation="MKIAFTAKGTGWDSPIDPRLGRTEYILLYDEDKDELSVTDNREI
                     EGVAHGAGPKTAQLLFDINPDVLITGNGPGDNAAVVLKQIPMKIYVGAGELTVKTALE
                     AYKNNELKEF"
     gene            44559..45923
                     /locus_tag="PLG69_00620"
     CDS             44559..45923
                     /locus_tag="PLG69_00620"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPI42041.1"
                     /translation="MVSFDVIIIGGGPSAIITGLTGKKQNPDMSFLMIKEEEKGLVPC
                     GIPYIFHDLGDVSKNMMGPAPFVAAGGEVLIDTVTEVSFKAKTLQTKSGLTFAYDKLV
                     FATGSVPIVPTFIKGYDLEGVEYIRKSYEYIKALKQKTDLAKNIVIIGGGFIGVEVAE
                     QLAKFTDKKVSLIEMQEYCLINAFSPRLTKLADTAVREQGVNLYTSAKVTEIIGQAGH
                     VAGVKLADGTEIPAELVIQTIGYTPNTMLAGQSGLEMNSKLAILRDNYMRTAVKDVFA
                     VGDCSESIGFVTGRPDNVMLASTATAEARILGYNLFKIGLIRTFSGTLSVFSTQIGSK
                     VFASCGAIEQTAEAAGIEYISGEFEDWDRHPESLSDASKLWISLVVSPKTGVIIGGEI
                     YGGKSAGEIINIISLAIQKGVTVYELVSYQIGTHPLLTTAPTKYVLIKAAEFAMSKIK
                     LFGN"
     gene            45945..46394
                     /locus_tag="PLG69_00625"
     CDS             45945..46394
                     /locus_tag="PLG69_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPI42042.1"
                     /translation="MPRVKLLRMLDDLPIIKGFRPIWMKANLRQAVIMNLEEYEALRL
                     IDYEKQIHEQAASTMNISRPTFTRIYETARQKLTTALVEGRSLLIEGGNVTIQESHWY
                     CESCFHKFSTAIASEADEMACPKCNSKQLLNLNDCFLSGCRRCRRCR"
     gene            46630..46953
                     /locus_tag="PLG69_00630"
     CDS             46630..46953
                     /locus_tag="PLG69_00630"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPI42043.1"
                     /translation="MPYHDGTGPFGDGRPGRGLGPCGRFGAPVGGGFGRGYGRRLGRG
                     FGGGYGRGFGFRNYGYYRDIPGYYNPEAKQDIYPYTREDLEAQKDELEKQIKWVNERI
                     AETEK"
     gene            47026..47157
                     /locus_tag="PLG69_00635"
     CDS             47026..47157
                     /locus_tag="PLG69_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42044.1"
                     /translation="MPNRDGTGIAGSGRPGRGMGPCNRAVSDKKVWEKALRKGTKKR"
     gene            47372..48190
                     /locus_tag="PLG69_00640"
     CDS             47372..48190
                     /locus_tag="PLG69_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPI42045.1"
                     /translation="MEDNEKKEQSLKANLQKIKHRIVVMSGKGGVGKSFIAVNLAYGL
                     ALQGKKVGLLDVDIHGPSLAKLMNIEGIPFPINQAGDMPMPVKVLSNLSVLTVATMLQ
                     AEDNALIWRGPLKMALIKQFFTDFEWEELDYLIVDCPPGTGDEPLSVIQTLEHVDGTV
                     MVTTPQDVALIDVKKSVSFSEKMNVPILGIIENMKYFVCPHCQKPTNIFAGNKIDELI
                     FEKSIDLLAELELDPNIGIASDKGRPYIYDYNKLSNAEKLMEAVLKIIAKVEIA"
     gene            48191..49132
                     /locus_tag="PLG69_00645"
     CDS             48191..49132
                     /locus_tag="PLG69_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPI42046.1"
                     /translation="MEYLFGPVPSRRLGVSLGVDIVPHKVCSLNCVYCEVDRTTNLTI
                     KREEYVPIDAVIKELEGYLSQKPELDFVTFSGQGEPTLNSGLGKVIDFIKDNFSLYKV
                     AVITNGTLFWDKDVRDGVNKADVLLPSLDAVSPITFLKLNRPSNELHIDRIIEGLIKL
                     RQEFRGKIYLEVFLVPGLNDTESELVLLKNAIKKIKPDLVQLNTLDRPGTELWVTPLS
                     QKKLRDILVFLKPLHVEIISKPQTRKQIQSFNENISDGILETIKRRPATDKDLCKILN
                     LNLNELNKYLSDLLESGEIETEEMNRGVFFKIKPRNK"
     gene            49226..49858
                     /locus_tag="PLG69_00650"
     CDS             49226..49858
                     /locus_tag="PLG69_00650"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPI42047.1"
                     /translation="MGDKFPDMKVQTTRGEITLPGDMKGKWFVLFSHPADFTPVCTTE
                     FVAFQKRYEKFRELNCELIGLSVDQVFAHIKWEEWINENLDVEIEFPIIADTGAVANK
                     LGLIHPGKGTNTVRAVFIMDAEGIIRIMLYYPQELGRNMDEILRAVKAMQIADKYGVA
                     MPANWPNNEIMNDHVIIPPAKDIKTAKERMEKAKTGEYECYDWWLCHKKL"
     gene            50096..50338
                     /locus_tag="PLG69_00655"
     CDS             50096..50338
                     /locus_tag="PLG69_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPI42048.1"
                     /translation="MTDKKVTVFSTPTCSWCVRVKDYLKKNGVSFSDLDVSKDYKAAQ
                     DMIRKTGQQGVPQIWVGSSAVVGFDKTKLDSILELN"
     gene            50496..50915
                     /locus_tag="PLG69_00660"
     CDS             50496..50915
                     /locus_tag="PLG69_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPI42049.1"
                     /translation="MENPDATATEGSPACGDQVTMYLKIEPDKHTIEDISFLSYGCAS
                     NIATASIITDLAKGKTLQEAKKITWHDAVEALDGLPPEKTHCSVLASDTLQAAISNYE
                     ITHGFKEVVNFNKDTILEELEKVIYPQVGEDTFPSSW"
     gene            51138..52316
                     /locus_tag="PLG69_00665"
     CDS             51138..52316
                     /locus_tag="PLG69_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPI42050.1"
                     /translation="MKKDAKFNTKLIHAGHKPEPYHSVITPIYQTSTFSFESAEQGED
                     CFAGDSDGYIYTRLGNPTTRALEDAVASLEGGVGGIAVASGMAAVTTIYMAYLGAGSH
                     IVSSAAVYGPSRVVLEKHFSRFGVESTFVDTSLIDLVAAAIKPNTKLIYIETPANPTI
                     AITDIVSCAELAHKHGILLIVDNTFCSPYLQRPLELGADVVLHSTTKFINGHADVVGG
                     VIVAKTPEAHKFLYSVMTNMGGNMDPHQAFLVSRGLKTLSIRMERAQENAMKLAVYLE
                     QHPKVAWVLYPGLTSHPQYELAKKQQDGPGAMISFGLKGGLEAGRTLMNNVKLAALAV
                     SLGGVESLIQHPASMTHAKMSYVDKQKAGITDDLVRFSVGIEDYEDIVEDIQQAFTYV
                     "
     gene            52788..53279
                     /locus_tag="PLG69_00670"
     CDS             52788..53279
                     /locus_tag="PLG69_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42051.1"
                     /translation="MLDKTLAIDKVELDNLEKTVCIDDLLQPVVHLRCPKYKVSLFVR
                     EKIHDELMDFLGGKGINPKFVPGDINYRYDKLSAIAAVIYQNRYRDELYRKEQNNPVI
                     AAMKFMDKGRNDRLYCREIYFEEEKQVIICEIHSGKKNQENRKKEKNIIKKVAGYEFK
                     IQS"
     gene            53257..53691
                     /locus_tag="PLG69_00675"
     CDS             53257..53691
                     /locus_tag="PLG69_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPI42052.1"
                     /translation="MSSKYNHEKIKQSIQGNLRRYEAIALSMVISFEIEDFIKERGIS
                     KQELAKAAGVSPSYLSQVFAGDRLLNLTMLAGISQKYQVKFHCDIQEAKVESIREYAF
                     ESFGKPHTKKANIYNFEDFKQVHNDKYNNQNRVIGSDCGNPA"
     gene            53675..54124
                     /locus_tag="PLG69_00680"
     CDS             53675..54124
                     /locus_tag="PLG69_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42053.1"
                     /translation="MEIRRSELQQEMFVVLDTQIYIDLPDDTKSKNIDDLDIVLKIYK
                     NNEDDHLVRIYYEIKAKKTYPDKPGLCIRVQASGDFRIDASIDVNSIQFGQLVNFSAT
                     AITYNNIRAYLQNITSYYPVDTYILPAIDLNDLCKKYYVNQKEENQE"
BASE COUNT        16898 a        10166 c        11508 g        15874 t
ORIGIN      
        1 cgctgtgtag atgtttgccc gatgaatttg gttccctgta tgattgtttc tgccgtaaaa
       61 tacgataatc aaaaacttgc tgtcagatca gggctgaatg attgtatgaa atgtggtgcc
      121 tgtgcctatg tttgtcctgc tcaaattcgt ttggtgcaat atattgatac cggcaaaatt
      181 cgttatgctg aagcaaaaaa gtaggtgaag aaaatgaaag atagatttat tgtttccccg
      241 gctcctcatt ttcacgataa aaccacaact aaaaatgtga tgtggaatgt ggtgctggct
      301 ttaactcccg ctttaatttt cgctatttat tattggggtt ttaattcgtt gttattaact
      361 ctgttgggcg ctgtttcagc gatggtcaca gaggcaataa ttcaaaaatt tcgcaaagtt
      421 ccgattacgg tttccgatgg cagtgctttt ttaacaggta tgctgcttgc ctataatatc
      481 aatgctggag ctccctggtg ggttcctgtt ttgggagctg cctttgccat tgctattggc
      541 aaacaggttt ttggcggttt aggcaataat cctgttaatc ctgctctttt aggcagagct
      601 tttttaatgg cttcctggcc caccataatg acagccggat gggcaaaacc taaaagtgga
      661 accttaagcg gaataaccga tccgaatgtg atagctgtaa acctgcaaaa tttatctccc
      721 aaggcatatg atctggtaac aggcgctact cccttaaaag tagctcaaac cttaagggac
      781 agcagttttg tatcttcgct tggtgcaaat gccggtcaag gttttgactt ggcaaatcgt
      841 atttttacca acttaaccga tttgtctgcc ttaaaaaatc ttttctgggg caatattggt
      901 ggttgcattg gtgaagttag cgtatttgct ttgcttttag gtgcggctta tttgctttat
      961 aaaaacatca ttgaatggcg aatccctctt ttctatattg gaactgtttt tgtgctctct
     1021 ttctttttgg ctccgctgaa aggttcaggt ttcagtccgc tattgccgtt tttccatatt
     1081 ttttccggtg gattgatgtt aggtgctttt tttatggcaa cggattatac aacaacaccg
     1141 ctaacaaaaa gcggaaggat aatttttgcc atcggttgtg gaattttaac tatggttatt
     1201 cgtttatggg gtggttatcc tgaaggagtg agctatagca ttttgtttat gaatgtgttg
     1261 actcctttga ttgataaact tactatgccc aaagcatttg ggagggtaaa aaaatgaaaa
     1321 tatatttaca gcttggctta atattacttg ccttttgtgt agcggcaact gcacttttgg
     1381 cttatgtgaa ttcccttact aaacctcaaa ttgattccat acaacaaaaa gaagcggaag
     1441 aaacccgtcg ccaattgatt ccggatagcg agtttgagga aattaaaggg gacttcacct
     1501 attacatagc taaagataat aaaactaaag aagtgaaggg ctatgttttt acggcagaaa
     1561 aaaatggcta taacggtccg gtaaaaacaa tggctgcaat agataaggat tttaatatta
     1621 tagctattaa agtaattcaa caaacagaga cacccggctt gggaacaaat tctacccaac
     1681 ctaaatttac cgatcaattc aaaggtaaat cggttgaaca attaattgta gataaagatg
     1741 gtggtgttcc tcctaattgt attaaagcca taacaggagc aactatcact acccgggcag
     1801 taaccaattc cttgaaagaa tccataacat tgttaaaaaa actggttctg gaaaaatctg
     1861 cctctaccat agaaacacaa accgaagaag aggtgcacaa atgagcttta tgaaagaatt
     1921 aaccaagggc ataataaaag aaaatcctac tttcgtgatt gtattgggga tgtgtcctac
     1981 tttagcaact tccacatctg tgaataatgc tatcggaatg ggattggcag caacttttgt
     2041 attgatttgt tccaatatct ttatttcgct gattaaaaat ataacccccg ataaaatccg
     2101 tattcctgtt tatgtaattg taattgcagc tttcgtttcc attgtggata tgtgtatggc
     2161 tgcttattta ccggctttgc ataaatcctt gggtctgttt attccgttga ttgtagtgaa
     2221 ttgtatcata atgggtagag ccgaagctta tgccaataag aataatgtga tcaattccat
     2281 tgccgatggc attggaatgg ggctgggttt tactcttgct ctatcggttg ttgctacaat
     2341 ccgggaaatt ttaggtaatg gcacctggtt gaatatgaga gttatgccta aaacttatga
     2401 tccgatgctg attactttgc ttgctccagg tgcttttatc actttaggac ttttgatggc
     2461 attgatgaat atgctgaagg agaaaaaata atgggattcc ttggacaact tttcacaatg
     2521 gcaatttctg ccattttaat ccagaacttt gttctttccc gtttcttagg tctttgccct
     2581 tacctggggg tttccaaaaa aatggattct gccttcggaa tggggatggc tgttattttt
     2641 gtgatgacct tggcttctgc tttttgctat ttgatttatc aatacatcct ggttcctttt
     2701 gaactggaat atctgcaaac cctggctttt attttgacaa ttgcagcgtt ggtgcagttt
     2761 gtagaaatgt ttattaagaa gaactctcct gctctgtata aatccctggg cgtctatctt
     2821 cccttaatta caaccaactg tgccgtttta ggtgtggcta ttctgaatat tactcctttg
     2881 gcggatggaa cgcgttacaa tttcatttgg gctgtcttaa acggctttct ttcaggcgtt
     2941 ggtttcactt ttgtcctttt ggcaatggcg ggaattagag aacgactgga aatggtggaa
     3001 atgccaaaaa gtatgcgtgg aatgcccagc ggtttaattc ttgccggaac aatggctttg
     3061 gctttttacg gttttatggg aatgaagttt taaaggaaag gtaaaatatg ctttatctaa
     3121 ttgttgttcc aatggctgca accggttttt ctgccatttt gttgccggtt gctattatgg
     3181 gttctctggg tttgattttt gggctaattc ttgcctttgc ttccaaggtc tttcccgtta
     3241 ctgttgatga acgcatagaa aaaattatag aggtcttgcc cggagctaat tgtggcgctt
     3301 gtggtcaacc tggatgttca ggatatgcaa cagctattgt gaatgaggga aaggacttga
     3361 atttatgcgc tcctggaggt cctgctgtag tagaaaaaat agctcaaatt atgggtaaag
     3421 aagcaactgc tatagataaa aaaattgccg taattcattg cagcagtgga ggtaaaaata
     3481 atactaaatg gaaatatgct tatcagggaa ttgaaagctg cctttctgca gtaaatattg
     3541 ccgatggacc caatctttgc tcctatggtt gcgtcggttt caatgattgc gtagctgctt
     3601 gtaaatttga tgctatcagt attgataatg aaggtatgcg ggttattgat agagaaaaat
     3661 gcacaggttg cggcgcttgc gtaactgcgt gcccgcgaaa attaataatg ctggttccag
     3721 caagtatgaa tgtgtttatt tcctgcagtt ccaaagataa aaatcctttg cccaaacaaa
     3781 attgcggagc cgataaaccc tgcattggct gtggtttgtg tgccaaaaaa tgccctgctg
     3841 aggcaataac tgtagagaat aatatcgcca ggattgatta cagtaaatgt accgattgtg
     3901 gaacctgtgc tactgtttgt cctacaaaag ctattataga cctgaaagca ggttcacgac
     3961 cggaagcttg atttcctcgt taaccaccac aaattcctga aggttgattt ccttatcaac
     4021 tacaggaaat tacacttcct gcagcttgtc attcctgtga aagcaggaat ccagaacata
     4081 aactacatag taagattgaa agtaaaacga cgaggacatc gttcttcctg taagtaagaa
     4141 accattagca ccttcagaat attattcctt tctatattct gaatggtgct tatgatttat
     4201 aaaggcgcaa aaaaggagct aacttaccac ttctctatct cattatctca cagataaaag
     4261 atttgacaaa aaagcccaat tgtaaaggtt gacattgaac tttactcttt aagggcaagg
     4321 ggaaagcaag gtctataggc atcctaaact aaaaattatg atggaagtaa aatgcgaact
     4381 tttgatgctg ttcttcctta tttgaagaag agctacaccc gaattttggg tggactggtg
     4441 atgttaattc ttgtggatct tgtgcagctt gcggttccga aagtaatgca atatgccata
     4501 gatcatattc aggaaaggac tatagatcaa cagggtctgc tttggattgc tttgattatt
     4561 tttgcctttg ccatcgcggt gatggtttta aggttttttt ggcgagttct gatcattggc
     4621 aattcacata gtatagaaaa aagtttaaga caggattttt acgatcatct gttaacctta
     4681 tcccagaatt ttttcaataa aagcaaaaca ggtgacttga tggcttatgc caccaacgac
     4741 ctgaatgcag ttcggatgct ctttggaatg ggtttaatat cggcaatgga tatagtttta
     4801 atgactgttg cttcttttgc ttttatgatc tctatcaatg gcaagctgac tttgctggca
     4861 gttattccta tgcctattat atcattaact gttatgtatt tcggcaaaaa agtgcatacc
     4921 cgttttaaag ctgtgcagaa aagttttgct actttaagcg gtagagttca ggaaaccatt
     4981 tccggtatca gaatagtgaa agcattctgt caggaaaaaa cggagctaaa aaaaatagat
     5041 gatgtttctc acgattttgt aaatcaaaat attgctcttg ccaagattgc aggtgtgttt
     5101 catcctttta tgggttttat cgtaagcata agtatgatta ttactattta ctttggaggt
     5161 aaatctgcta ttcggggaga aataagtgta ggtggcttca ttgctttctt ccaatatctc
     5221 ggaatgcttg tttggcctat gattgctatt ggttggattg tagatatata tcaaagagga
     5281 actgcttccc tgaaacggtt aaatgaaata tttgaaataa agcccgaaat tgatgatgtc
     5341 tttgcggatg aacgcataac gcatctggaa ggtaaaatcc tatttcagaa tttatctttc
     5401 cgctatagcg ataatctgcc tttaattttt aaagacatca cagcaggtat tgaagtagga
     5461 aaaacactgg caatcgttgg tcctacaggt tgcggtaaaa ctaccttaat tgaactttta
     5521 gtaaggatct acaatcctcc gcgaaatagt atttatgtag atgattatga gctgtatact
     5581 attccgctga atgttttgcg tagagatatt gttcttgttc ctcaggatat tttcctgttt
     5641 tcagaatcta ttgcagataa cattcgtttg ggtaatcctg atgcttccga tgaagaggtt
     5701 tttgaggcag ctcgtattgc ccaggtttat gatgaaataa tggaatttga acagaaattt
     5761 gaaaccgttg tgggagaaag aggtgttact ctttccggag gacaaaaaca acgcgtagct
     5821 attgccagag cgttgcttac caatcctaat atattgattt tggatgatgc tctttcagca
     5881 gtggatacaa aaaccgagcg gcgcattctg aaccgcttaa ttgaaattcg gaaagggaaa
     5941 acaactctta tcattgccca ccgcatttct tccctgcaac attctgattt gataatcgtg
     6001 attaatgaag gtattattgc agaacaggga actcatcaaa accttctggc taaaggtggg
     6061 ttgtataaag acctctacga aaaacaaaga attagagcac gcttggaagg agaagaagaa
     6121 taatgcatgg tggtggtggc ggtggcggtc ctatgggaca aggtgcctat tttgaagatg
     6181 aactgaaaac caaaatctac aataaacggc ttttcagtaa tatgcttcgt tacttgaaac
     6241 cctacctcaa gtgggttatt ctttctttcc tatttttaat gttgatttct ggtgccgaag
     6301 ttattattcc cttaattcaa agatcggcta ttgatgatca tatcgtttcc gataagtcca
     6361 ttgcagtttt taccggggaa aatgattatc aaaattttat aaaacgctat gaaaaacagg
     6421 gattcaaaga atataaatat ggtgaaaatc actttgtaat tatcaatgct aaagaccgta
     6481 ataaaataaa tcgtactgat ctggagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnncttt gtaattatca atgctaaaga ccgtaataaa ataaatcgta ctgatctgga
     6661 gtacttacaa gaaaaaggaa tcctaaaagc agaaacggta tttctgctaa gcgataagga
     6721 tgagaatatt aacatcttaa agaagtatct gcaggaaaat ttacatcctg atacgccaaa
     6781 tctttccgga tggtataaaa tcggctcaaa aacgattgca gttcccaaaa aagaactaaa
     6841 caaattaccc aaggaagagc gtttgaaagt gcggaatgag acagttcaaa agttgttgat
     6901 tttagctctt atctttttgg ctattattac tttacgcttt attgccagtt attttcaaac
     6961 tgtctttact gcctattttt cgcaaaatgc aatggctgat ttacgacacg atgtttttgc
     7021 ccatctgcaa aaaatgccaa ccaaattctt tgacacaaat cctgtagggc gtttagttac
     7081 gcgggttaca aacgatattg ctgccattga tgaaatgttg gcttcaggag ttataactct
     7141 aattcaggat gttattttaa ttattacaat tgtgatatta atgttagctt tgaactggcg
     7201 tttggcttta gtgagtttta gcatcttacc tttagtaatt tgggtaattg ttattttccg
     7261 gaaaaaaacc agagttgtct atcgggaagt gcgaaaacat cttgcagttc taaatgctac
     7321 tttggcagaa catattgaag ggcagaaaat tatccggctt tttaatcaat actttcataa
     7381 gcgtcaggaa tttgccacca tcaatcaaaa atattatctt gcttccatta agcagatacg
     7441 cttatttgct tttttccgtc ctatcattca tgtttcttct cagattgctg ttgctttaat
     7501 catttggtat gggggtggaa aaatactgca aaatgtaatt accatcggtt tattgatggc
     7561 tttcactcaa tatataagta aactttttga gcccataaac gatttttcgg ataaatttaa
     7621 tttcttacag agcgctttag cgagtgcaga aagaatattc aatttaatat cgcttacccc
     7681 tgatgattat agagtggaaa tgcataaaaa cataaaaatg aaaggcgaaa ttgaatttca
     7741 aaatgtttgg cttgcctata atgacgaaga atgggttctt aaagatatct cttttaaggt
     7801 tgctccggga gaaaaaattg ccctcgttgg gcatacaggc agcggcaaaa catctattgt
     7861 aaacctgatt ttgggaatgt atcctttcca gaaaggtaga attttaattg atggtaaaga
     7921 tatacataac tattcgttag ctgacttgcg tagaaatgta ggaatcgtgc aacaggatgt
     7981 tttcctcttt tccggaaata taaaagataa tatagctctc aataataatg accttaccga
     8041 tgaggaaatt atcagagtgg caaaatatgt taatgccgat aaatttatca gtcaattacc
     8101 tcggggttat tacgaaccgg ttatggaaag aggagcaact ctttctacag gacaaagaca
     8161 gcttattgcc tttgccagag tgctggctta caatccttcc atttttattc tggatgaggc
     8221 aacttccaat attgatactg aaaccgaact tcttattcag gatgctttaa ataaagttat
     8281 taaaggtaga acttctataa taattgctca tcgtctttct accattcaaa atgtagaccg
     8341 catcttagtg ttgcataaag gcgaaattgt agaagaaggt tcgcattttg aacttcttga
     8401 taaacagggc ttatattatg acttatacag gttacagtat acctgaaata gagcttgctc
     8461 agaaaaagtg attgacagaa ttgcgggttt gaaatagtat ataattatag ggattgagtt
     8521 tttgtcacca gattgacttt ttgtgctccc ttatatatat agagtgctta attgcggaag
     8581 aacgatatcc tttttggttg acgaggatat caaccttccg gagcaacaat aaaaagaaaa
     8641 aaacattact tgatctataa ggaggatcaa tgaaaaagta ttggctgatt atcttagccc
     8701 ttacactcgt aacagcaatc tttgccgacg gcaatcgcta tcaggttatt gcttatggtg
     8761 aggactttga atccggggct cctggctgga ctcattacga cgctacggtt tcacctaata
     8821 attggcatat ttacaatttt ggcggaactc agggaaatgt ctggtggatg ggagacccgg
     8881 ctctggctca aggaactaac attggtggtt attataatca tcaatacctt gttttggata
     8941 ccccggcaag aactttaact tctgctaatg ccactttaac tttcaaaatg cgtcacaatg
     9001 tagaaacccc gggttcaaat cccccttgga atggttggga tgcctgtaat gtaagggttt
     9061 ccactgatgg cggaactacc tggacaccta tttccggaaa tcctgcttat gattttactt
     9121 ccgcttattc cttcggtttt gaacatggcg aaggacaggg aattcccggt tggggtggag
     9181 ttcgggaatg gactaattgc acttttgacc ttagtgctta tgtgggacaa agtgtgaaaa
     9241 tccgttttgc ttttgcttcc gatccagctt actgcacaag cgatgcacct gcaatgtatg
     9301 gaatgatggt tgatgatatc agttttggtg gatacagcaa taacggagta gatgatggtc
     9361 aaatgaccta ttcttctctg gttcccgttg gagaagacct ttggcatata gcaactgatg
     9421 caacagctcc ttcacctact cattgtatgg tttgtcaaaa tgcttccggc agttataata
     9481 ctaatatgct gaactattta gtcagccctc ctattacttt acctaatagt ggagatatgt
     9541 gggtggactt tatggtgaag ggaagttaca atgatccgaa tgccttcccg gaagtggatt
     9601 attttggctg ggaaatttct gtagatggtg gaaatacctg gtatgcaatg agcaatcctt
     9661 acgggacacc cggaggaaat aattatgtgt atacaggcct gcaaaatacc tggcaatcta
     9721 tgaccgaggg atttgggttg agtttcttta ttaccgaata ccacggtgaa acagctattt
     9781 tccgctggta ttttaagtcc gatggtgata ctcctgatgg agtaggaatg tttattgatg
     9841 acttcagggt ctataatgat gtatttattg ctgctcccga aaatctggca gccgctgttt
     9901 ccggaaactc agttaccctt acctggaatg aacctggtag tggaggcgga ggtggagaag
     9961 agggctggat ttattactgt ggtgaaccag ccggaaattc tattggcacc aatggagttg
    10021 ccgattttag tgtagctgcc aaatgggatg ccattgggga aacgaatagt atctatcctt
    10081 atgtgggaat gaatattacc aagattaaat tcgttcctgc agagcccaca actctatgtt
    10141 cttatgcagt tcgcatttgg acaggagcag caggtgtatt agtttatgat caggcagttc
    10201 ctactattac tgaagatgta tggaatgaag tgatcctgac tacaccatat actattcccg
    10261 cagcgaccac aattatggtg ggataccgtt gcaatgcaca aggcggcttt cctgcaggtt
    10321 gtgataacgg acctcaagtt gagggttatg gcaatatgat ttatatgaac aataattggc
    10381 atactcttac tgctgaaagt gcttcgctta catataactg gactattcag gtttatgtag
    10441 ccgatgccac agggaaagaa tatgtgttgg gtgaattgcc aatgaacaat tcctttgcgg
    10501 aaggtaaatt tggtgttcat tataccgaac ctgtgagagg acgtgatgtt accggttata
    10561 aggtttatcg tgatgccggt tatgtggcta ctgttgatgg taatgtatta acttacaccg
    10621 atactgaagt ccctggtggt cctcattctt attatgtaac tgctatgtat ggaactgttg
    10681 aatccggtgc ttccaacacg gtaagtgttt tcattctgcc cgctgagcat tatgaaatca
    10741 atcacgatga cgggacagca gaacagggat ttagtgtggg tgctactaaa cagatggcag
    10801 ttaaatttga atgcaacgat gaatatgacc ttaaatatgt aaaagtgtat gttcacacct
    10861 tgggttctgc cggtcttgtt attcgcgttt atgatgctaa tggaccgaat ggaatgcctg
    10921 gagctcaact tgttacccag tatcaatatc ctgcagcttc agttgttgcc ggctggaact
    10981 ttattacttt gcccacaccg gttcatttga acaaccaagc attctatgtc gctttatttg
    11041 agacatctgg cgcttctcta attggtttgg atacaagttc aaacggcttc agttatcaaa
    11101 atacaagcgg ttcctgggta ccggttacaa ctggtgaaat tatggtgcgg gcaataattc
    11161 agggcggaaa tgcaggagaa gatcttactc ttcctccggt ggttctggat gccaggaatt
    11221 atcctaatcc tttcaatccc gaaacaacta tcagtttcag tattcctgcc agcggtcaaa
    11281 ccagcgtgaa aatctataac ctgaaaggtc aattagtccg aaccctcgtt aatgaaacga
    11341 tgaatgctgg ttatcagaaa gttgtctgga atggcactga tgataatcaa aagtcagtag
    11401 ccagcggtat atatttctat cgcgtaaata atgccggtaa aactattacc cgtaaaatgc
    11461 tgttagccaa gtaggtaaaa gatttattag gttaacaggt taacgagtta ataaggaaaa
    11521 ataatgaagc cccgaatttc ttcggggctt cttatatata attttataaa tgacaaatca
    11581 tctgtcattc ctgagaaaaa aggaatccct tcctttttga aaatgaattc cgcaacaagt
    11641 gcggaatgac aaagcaatat tccatctaaa aatgatataa tttaattcca atagtgcagg
    11701 agaaataata aaaagatata tttagcaatc gggttgccga attccgattc ggcaaatgct
    11761 aaaggcataa atttctttgg aaacctccag tttctttgtt aaagaaaata tttgccaacc
    11821 cattttccct caactgcatt atttgggttt aaggtaaaaa atatttcttt attttcgtga
    11881 taaaaaaaag ctaattagtt gcatcatata aagttaacag tccaaattgt tcatcttttc
    11941 ccgaacttaa gttcaaatgc gtaatttatt gaaacccaca agattaagta acgactccgt
    12001 aacacttccg taacacttcc gtaatttcgt tacggagttg ttacggaatt gttacttggt
    12061 cattaagcat tttagcgagc aaaaagaaag agctcgtaat ctttgtaaca ggcgggaatt
    12121 gttattgttt atagggggag ttagcaccga aattatgaat taaaaacatc tttagatttc
    12181 ttctgcatcc tgaccgtaaa tttctttaaa gcggatatta tcaatttggt taggaattcc
    12241 ttcaaagacc ttatgtcctt ctttcagagc taaaacctta tttccatacc tgcgtgccag
    12301 agttaaaaag tgtaatgaac agataataga tattccttct ctgtttattt ctcctaaata
    12361 ttgcataatg gaatcggctg tggcagggtc taaactggct accggttcgt cggctaaaat
    12421 taaagaaggt ttttgcatta aggcacgtgc aatgccaact ctttgttgtt gtcctccgga
    12481 aaggaatttt acctgtttat cggcaaaatc ctgtaagccc actttttcca gattttgttt
    12541 tgccagttca taatcctctt tgctgaaagg tgaaagagag gaatgatatc ctaagcgtcc
    12601 ggttaaaaca ttggtgagga cggtgaggtt tttcactagg ttgaattgct gaaaaaccat
    12661 tccgatttgg cggcgataat gtttcagctc tattccttta agttgcaaaa tatcttcatt
    12721 gcgaaaaagg atttgtcccc tggttggttc aataagacga ttgatacaac gcagaaaagt
    12781 agattttccg ctgccggaaa gtcctaataa aattacaaaa tccccttctt gcacttttag
    12841 agataaatct tccaaggccc attttttacc gtcataactt ttgcttaagt tattaacttg
    12901 aagcaaatta ctgtttggat tcatttaagc aattcagctg ggtccatatt cattgtctta
    12961 agggttgtgc gcacaatttc gtaatcctta tcggaggcat attccaagcc atcaatatca
    13021 taaagagctt tcagaacctt tttcccttcc gcactttggg catagcgata taatgcctgc
    13081 acaataatac cttccagttc gggaggaaga tttttccgaa aagttaccgt atcattggga
    13141 atccaatcag tataatcaac tattttaact ttttcaaaca catcgggata ggtctccagc
    13201 agtttttctc tagcatccat tggttttcct ttggcatcag gtggcgacca ataactgcaa
    13261 cccacatccg ctcttccact ataaactgct aaaatagctt gcggatgacc tcccgcaaag
    13321 aaataatcct tgggcgtaat acccctttgt tttaagatag cggaaggata aatatagccg
    13381 gaagtggaag cggcatccgt gtaggcaata atttttcctt caatatcctg cagagaatta
    13441 attttactat cactacgggc tacaaactgt cccctgtatt tggttaagcc attgcggaca
    13501 gtcatcaatc gcacttgcgc atcatatttt tgtttagcta aaatataagc ataagttggc
    13561 aaccaggcaa catccgcctg gtaagttccc agtgcttcaa ttacggcggc ataacttgtg
    13621 ggaacagcta ctttaaaatg taatttggtt tctttttcca gataattggc tattgcttct
    13681 ccgctggaaa caacttttcc tgcttccaga gaaggaacaa aatacatttt aatcggattc
    13741 tgtttagaac caagtttttt attggctttt tccaatcggc tttcttcggt tccgcaacca
    13801 aatattaaaa gtgccacgaa ggcaagccag atatactttt tgcacattgg cataaactcc
    13861 tcaatgaata atttttatct tcttttccta tagagccaaa ctcggtcaag aaaaaactac
    13921 agataatccc agtcctcaaa aaattggtca atcagcaaaa taggattttt ccatgtttcc
    13981 tgaaaagtaa gatggtcttt tccatccaag gtctcgcctt cattgttaaa aatgctggca
    14041 ggcaggataa ttgtttcccc tgcttgtgga gcaagctgtt gccataaatc cgaataagtt
    14101 atcaatccgc aaacacttat atcgggaccg aaaaaatcgt tgtgaataac ctgaacgcga
    14161 ataaactggt tttccaatcg cttattcagg tcttctgcta tgcgagtgat aacattttga
    14221 gcacagacgg aactgatcat taaatagtta accggttttt ttctcagctc tttcaacaga
    14281 ccgcgttttt tttgtttata ggtttgaata gttaaacgca acatcccgat tccgttttcc
    14341 aactggggat aatcggcata aaaatcctca tcgggaatat ctctttccgc taaaatataa
    14401 aattcatctg caggataaat aattgtgctg ttatattcct ttcttgcttc atcaatcaaa
    14461 gctaaaattt ctgtagcggt ttgccgatta taaggtataa ttgcatcaag gtttttacgg
    14521 tatttggtta agcccacagg aacgattcca atggaaagac aattaatttc tgaatcgtga
    14581 aggtctttca aggtctgttt taatgcctct ccatcattaa tacctgggat gcaaacaatt
    14641 tgaatatgga aacctattcc gtgccgactc aattttttta acacagataa ggcatctacc
    14701 tgttttgagg gacgcattat cttttgtcgc aatgcattat ctgttgtatg taaagacaca
    14761 tacaacgggg taattctctg attgataatt cttgcataat cggtttcatc aaggttggtt
    14821 agagttatgt aattgccgaa aacataagaa tataaatagt cgtcatcttt tatgtataag
    14881 gtgggtctta attcaggcgg catttggtca ataaaacaaa aaatacaggc gttttcacaa
    14941 tagcgaattt tgtatggctc gggttcaatt cccaaaaaac ttttttcttc tctttgaatt
    15001 ttaactttac gagagatacc ttctgcagac ataatttcca gctcaaattc atagtccgaa
    15061 gtataaaatt ccaaatccag aaagtcattt actggctgtc catttacgga aagcagtttt
    15121 tctccgcttt taatgtcatt tttggctgcc aaacttcggg gcagaacatt ttgaattaga
    15181 atagccattg tttttctctg ttttcttttt taggtgtttc ttatcctgtc gtaatggaac
    15241 ttaaaacgct tgatgccttc aaaaatagtg cgtgccagac gatcctgata ttcttcattc
    15301 accagtaatt gttcttcttc aggatggctg ataaagccca tttccactaa aatagaaggc
    15361 ataaaagcac ctcgcaacac ataaaaattt gcctgtttta cacctctatc aatactttgc
    15421 gtgccggcaa tcagattttg ttgcacattg aatgccatat tgttactgtt ttccaaatgt
    15481 tctgtctgag caaggtcgct gaggataaag gaaaggtcat cgtatttctt ttttgcttcg
    15541 cttcctcctt caaagcgttc tacaacagca ttttccatag cttccacggc tctggcatcg
    15601 gaggtttgag cagtggataa ataatatgtt tcaattcccc gggaagttgt tgacttactg
    15661 gaattggaat gcaaactgat aaagaggtca gctctttttt cattggcaaa acgggttcta
    15721 tcatataaag aaacgaaacg atcatcttcc ctggtaagta aaacattaac ccccagttct
    15781 ttttccagca agttcttaag tttcaaagca acagcaagat taatatcttt ctcattggct
    15841 ttcagttttt tccctatggc tccagggtct ttaccgccat gccctggatc aagaacaatg
    15901 gtctttacgc tattatcaat tggtttggcg atttgtaaag tgcttccttt gcgttgaatg
    15961 agagagggaa aatgtgtctt aagttgttca gttacaaaaa cagaaggcag atataaactc
    16021 tctcctttcc gtaaaagagg ataatgcata ttgtaagaaa cagttttcag agtataataa
    16081 ggtgaatttt ccaggaagat aaactgttca tcatagaggt tcaaatacaa tctattgtca
    16141 cttctatcct ctttactgat ggctttaaaa atagcgctga aatcctttaa agctacataa
    16201 gaaacagaag aaagcgttgt ttcttgcaaa ttaacaggtt ttgcttcgcc cttaatttgg
    16261 atagtgatat tacccaaaag cattcctaaa caggtaagca gggcaaaaaa gacaaagaat
    16321 ttctttttca tagtgtaagc tccaattggg gattaaattc cgaaacggga tttatttcct
    16381 ccggtgcagg agaaaaattg ggagtccaag tatccgaact ggaaatttct tgagtgtata
    16441 atttggtaaa accaagttct acagctgtat caactacctc aaagtattcg gataaagtaa
    16501 ttcctcgtgt tagttcttta tagttttttg cttttccggc aggataatat tgtcccataa
    16561 gagaaagagt tagctccgtt cccaattctt ctgttagggt gtataaattt tctttgcaac
    16621 cggctaattt attgggtaaa acaagcattc tgagcagcaa tcccttttgg gcaattccct
    16681 tctcgtcaag ttctaagagc cctacttgag aaaacatttc tttgatggca gaaatggcta
    16741 cggcaggata atctggagca gaagaatatt tttgggcata aaccttatgc gcatacttga
    16801 aatcaggcag ataaatatct accagtccgg ttaaaagttg cagtgtttct accttttcat
    16861 aagcagaaga attccacagg acaggaatag ttaagccctt ttctttggct tgaataatgg
    16921 cttccctaag ctgaagagta aaatgtgtag gagttaccag atttatgtta tgagctcctt
    16981 cttcctgcaa ctttaacatc agacggataa gctcttcaac tgatatatct tttccccaac
    17041 ataaagtaga aatctcataa ttttgacagt atacacagcg taaattgcat cccgcaaaga
    17101 aaatagtgcc acttccgcgt gttccgctaa aacagggctc ttctccgaaa tgaagtgtag
    17161 ccaaatttat tctaagttta tccgtaacac cacaataacc tttgtttttt gctctctcaa
    17221 ttccacaatt gcgtgggcaa atattacagg aaaacatttg ggaataggac atttaatcct
    17281 tcttcaaagt aaaagcggag ggaaataatt ctgttattaa agctttgtga attgccttcc
    17341 ggttagcata tattatttca atctccggtg caaattcata aattacttgt ctgcaagcac
    17401 cacagggagg aaaactttct tcgctatcca cataaacagc catttccgaa aaagttctat
    17461 gtccttcact gattgcttta aaaatggcat tgcgttctgc acagatagtt aaagaataag
    17521 aggcattttc tacattgcaa ccggtgaaaa tatgtccgtc tttagttttt acggcacaac
    17581 cgactttgta atgagaatag ggagaatagg agttttctgc tgcctttttg gctgcctcta
    17641 ataattcttt ttcactgaga tttaattcta ccatctctat cctcttttta agccaatagc
    17701 gctaaaatta agccaccggc aataacagaa ccgatttgac cacctacatt tacactcact
    17761 gcaggcatca ataaaaagtt gaaagggtct tcctttagcc ccatttgatg aataactctg
    17821 gcactcatag gaaaagcgga aattccgcaa gcaccaatca tcgggtttat cttttccttt
    17881 ttgaatacat taagtaattt ggcaaaaagc actcctccgg cagtatcaaa aataaaggca
    17941 acgagcccta aagctaatat taacaaggtc tgcggaacca gaaaaagttc accttgcatc
    18001 ttggaagcaa tagttaaacc caaaagaatg gttactaaat tccccagttc attttgagcg
    18061 gttttgctaa gcccttctaa aacaccggat tcccgaatta aattgccgaa cattaaaaaa
    18121 ccaattagcg ctaaagcagc aggaacaaat attccggcaa taatggtaac agcaatagga
    18181 aacatgattt taacaatttg cggaacatca ccactgtgat aatccatccg aattaggcgt
    18241 tcctttttgg ttgtaagtaa acgaattaca gggggttgaa taatcggcac taaagccata
    18301 taagaatagg cagcaacaga aattgctcct aaaagattgg gagcaaaacg attggctaca
    18361 taaatggaag taggtccatc tgctgctcca ataattccaa tactggctgc ttccttaatt
    18421 tcaaaaccta aaccggcagc taaaacgata gttacaaaaa tacccaattg agctgctgcc
    18481 ccaaaaagca gcataaaagg atttttcagt aaaggagtaa aatcaatcat tgctccgatg
    18541 gcaataaaaa taagtagggg aaataactct gtatcaattc cggctttaaa gagaatacct
    18601 aaaggcgaat gctctcccac agcagcagaa aacggtatat tggctaaaat agttccaaaa
    18661 ccaataggta acaacaaggt tggttcatat ttatgtttta ttgccagcca aatcaatatc
    18721 agtccaataa taatcattat aacctgtttc agttggaagc caagcaagcc ggaaaagagt
    18781 gtttccatta gcaaaactcc tatgtcttat tgcatttgcc actatcctga agagggattg
    18841 tttgtcaagc aataacacaa aaacaattcg ctcactgatt tcatagataa agaataagct
    18901 cttacagatt acacggataa agaataagct ctcacagatt acacggatta cacagatttc
    18961 tattttataa taaaccataa gaaaaaatcc agttgatcaa gttatctgcg ttctattttt
    19021 tctaccctct ctacctcaag acctcaagac ccaaattttg gttgacacaa tcttaaacat
    19081 cttttttatg aatttatgag aaatatcgtt ttaattctgc tggttgctct tttactgaca
    19141 ggttgtgatc tgttttcagt tcgtgattca gaaccacctg cagcacagat accgtggata
    19201 gattttacca cagaggttga ttctgtgttg ttaaatctgg aatacagtta tgaatatcct
    19261 caaaatgcag ttaattatgc acggcttttc agttccgatt atgtatttca ttttgccccg
    19321 caggatgtga gcgatttttc tatagagcaa acctggacaa gagttcagga gcaggatatg
    19381 attcaggttt tgcataaccg ctataaaaat atgcaggtaa ccttagagaa agtggatgat
    19441 caggatcaga tagaatcggc gcaagcaaaa atataccgtc aatataatat tacaggtatt
    19501 tcccgcaatg agggaaagga agttttaact ttggcggaag ggaatatgga actgcattta
    19561 aaaaaggaat atggctactg gtatataaca aagtggtatg attaccgcag tagtaatgtt
    19621 cctacctggg gaaagttgaa atatgagaac cattagcatt attctgcttt ttttgactct
    19681 tctgattggc agttgcaaca atcctttctg tcctgaactc tatgatgtgt ccaatgagga
    19741 aattcaaaat cgtactcctg aagaactttt acaaaatttg gaacgagcat acaccgaaag
    19801 gaatatcaat atttataaat cccttttgca tccggatttt cgtttcgagc ttctggcaag
    19861 cgaagtaagt ttaattggaa ttgatatgaa taatgacgga atgcgtgatt cctggtgggg
    19921 ttatgcacaa gaagtggaat atacggatag aatgtttaat aagggttcca gtgatggaac
    19981 ttatcctcct ccggatgata ttaagttacg gttacaaatt cccccttctg aaagatggga
    20041 gaatgatccg gaagtaggtc atgaggattg gataataatt ccttgtaatt ttgatttaat
    20101 cctttcctat caggaaagca attcttctat tactgccaat ggcatagccc gattttactt
    20161 gaggcctgta ggaaacggtt ggaaaattgc catttggaga gatgaatcca atatatgagt
    20221 tctaagggac gcattcttgc cctggactat ggagaaaagc gtatcggtat tgccttaagt
    20281 gatccgaatc gtattttttc caagccgctg tgtgttcttt ccaataaagg ttttgaacaa
    20341 ttagtggagg aattgaaaaa actaattgct ttgcatcagg tcactttgtt aataattgga
    20401 attccttatg ccatagatgg ttccatcact cccaaaacgg aagagtgtca gcagattatg
    20461 aatcgcttaa aagatgtctt atctattccg gttgagggtt ttgatgaacg ctattcaacc
    20521 tgggaagcaa atgaagaact gaaaaaaatg ggatataact ggagacaagc aaaagaaatt
    20581 aaagatgcta tgtctgccgc aatgatttta aaagagtttc tgaaccaatg aaaaaagcaa
    20641 aaccatttct tatcggtttg gcaatcttat tgtgtctgtt aattttggtt attgtttatc
    20701 tggtttttgt ccctttgcat agtgaagagc gcattgtgcg aattaaacaa ggagataatg
    20761 ccagaataat tgcttctaaa ctttccgaag caggaattat tcgcagtaaa acaatgttta
    20821 ttattctcac caaaatcaga aaagcagata gaaacctgaa accgggaagt tatatttttg
    20881 gaggcaatac ctatctttgg caaacggtta gccgtttata taaaggtcaa aacgaaagca
    20941 taacgatcac ttttccggaa ggtctttcgc tatataaaac cctaaagaaa attgatgcca
    21001 gtggcttggc aacttatgag gaactgcata aagcagcaac aaatcctttt ctggtaaaaa
    21061 aacttaccgg ttttgatgcc ctatctttgg agggttttct ttatccggag acctatcgtt
    21121 ttccgattga aatatcgccg gattcaattc tggcaattcc cgcaggagaa tttttccgcc
    21181 gtttacagaa agaagggatt gatccttttg ctgttcccga tttttatgat aaattgatcc
    21241 ttgcttccat tgtggagaaa gaggcagggg atgaaagcga aaaagaaatt attgccggac
    21301 tttttttaaa ccgtatgaga cagcaaatgg cacttcaatc ctgtccaaca attgactata
    21361 ttttggagcc ccaaggaatt aaacgcagtg ttttaactta tgcggatacc caaattaatt
    21421 ctccttataa tacatatctt tatcaaggat tgccaccaac accaatttgc aatccttcaa
    21481 ttacaaccat aaaagcggtt ttgaatgcca aacctaattc ctatttatat ttcttttccg
    21541 atcgccaagg aaaaaatgtg ttttccaaaa cttatgaaga acatttggca aagcagagaa
    21601 cgatgctgta gagtttatga ggtcaaaagt ttgaaggtca agaggttagt agaaaggtaa
    21661 acccttaacg agcataggaa aaatggtgta gccggaggaa gcagttcctc cgcaaagaat
    21721 gaatgctgaa ctaggaaaaa gcataattca gagtaaaaaa ccgcatattg gataatttgt
    21781 ttcatatatt taagatgaat ggctgaaatt atgaaaacga acacgaataa acggaacttg
    21841 acaagatatt ggtttatggc tatattattt aagggagata taatttaatg cttggacctt
    21901 atgaaataaa tgatatatac caaaccgact gtgtagaagg tttaaaaaaa ctgccggaga
    21961 attgtataga cctttgtgtt tcatcacctc cctatgatgg aataagagat tatcatggtt
    22021 tttccttaga ccttcataat gtaggtatag aactttaccg ggtgatgaaa gaaggtggca
    22081 ttgcagttat ggttatgcaa gatcaaacca ggaattttgc caaatcatta actactttca
    22141 gaactgcaat tgactggtgt gataatgccg gatttcgttt atttgaaaca ctaatttata
    22201 gaaaatatgg agcagaaggt ggctggtgga aaacaaggtt tagagttgat catgagttta
    22261 tgttggtttt tcttaaaggg caacgacctc agtattttga taaagagcca ttaaaaatcc
    22321 cttcaaagca tggcgggaaa acccttacag gcggtggaac tcgtttaaca aatgggatta
    22381 gaattccaac cagacctatt ttaataaacg aaatgaaatg ccgaggaacg gtttgggaat
    22441 acttaacagc aggtgacggt tcacgcttga aacataaaca tcctgctact tttcccgata
    22501 agttacctta tgattttatt cagtgttttt gtccgccaga aggtattgtt ttagatattt
    22561 ttatgggttc aggaactaca gctcttgccg ctattgaact aaaccgtaat tttttgggtt
    22621 ttgaaatatc cgaagagtat gttaaacttg ctaaacagcg gattgctatt gaaggcaaga
    22681 aagacgaaga tcagcttatg tttctgtaac tttctattgt ctcttggtta attatatgtt
    22741 cttaaaaatg ttgtgagggt ctatagtata ttctattaaa ttaccgcttg tttgtgccat
    22801 agataaagga ttttcggaaa gaatttgtct aaagattcct acccgcgctt tattactttt
    22861 cttcgtaaat tgaaagacat caaaatccga tttagtccat ttgtcttgaa tatagttcca
    22921 ttttgtcttt gctgataagt atttcaggaa attttccgtt ttcaaccagg tattatcatc
    22981 aaagcggaaa taagaattgt ctttttggag accatcaatc tttccctcac gagtagagat
    23041 tttaatctta tcactatagt catcctggct tataacttta aagagttctt tcaatgctat
    23101 cattaaggat gtgattgtgt aacattcatt aaagaagatt ttatgcagag cagatgattt
    23161 cctgtcagcc cctaaataat gtaaccagct taaacagttc tcgggatagt tgaataagtt
    23221 cttaaaagta ccatctgtaa aaaggagagg acagatgctt tctgtttcaa tattaatttt
    23281 ttcctgattg tccaatattc caattagtaa atggatcatt gctaaaggat atttgttttt
    23341 aaaatgggtt tgaccataat agccccgcag aagatttggc tgaatacatt gatttaagtt
    23401 agtccataat tcaggaattg ccctttggga atataaaact atatggtgac ccgcgcttct
    23461 gataaagtcc ctaaatattt ccatatccaa aaaaatacat tcattaagat caattttttt
    23521 atcttttatc aggattttac catcataaaa gccaactatt ttccaattta gataattaga
    23581 cataaacaaa ccacataata atccatccga atcagggctt aaaatgcagt tatgatctct
    23641 ttccaaaatc cagctatatt tcttaattag ttcttgataa ttgaggacat ctaaattatt
    23701 ttccaaagct gactccttgt aaattttaat ctccttaact ttaaatttgt tttgccatca
    23761 tctgtcaata attatagtca taacccaatc aatcttgtaa tttggaaaat tctgaaatcg
    23821 ttattttttt ttgtgtttgg tattttgaac ttgacatata cctgatgatt gattagattg
    23881 tatatttgac tatagagtat gaaaagcaaa aagaatattt aaccctaaag ggaggaaaaa
    23941 tgaagttaaa caagatgatg ttactcttag ttttagtggc tgtagttgtg atgattgcat
    24001 cttgcagttc aaacaaaaaa gccaatatta aaccacaagg tgaggaagtt taccgtccag
    24061 cttggtgggc tacccaacct gaacaggatt atgtttgtac ttatggacaa gcaaccaagg
    24121 tttcggaaaa tttatcagtg gaagcagcca gggcaaatgc tcttcaggaa gcagcacagt
    24181 atgtagaggt tttggtgaat agtatgctga aaaattatga agaagaagca ggtgttaaag
    24241 accctcaggt tttagctctt acacaaaatg tggtaaaagc cgtttcttct gcaaaattta
    24301 ccggtgttct aatgggtaaa attgagacca gaaaggttaa tgaaccagat ggtgttcgct
    24361 ataaaacctg gattcagttg aaaataccta aagatgaaat caacagaaaa ttggcgaaca
    24421 gcattcgcaa cgaagaagca ctttacaatc agtttaaggc atctcaggca tttcaggagc
    24481 tggataaggc agtagaaaaa tactaacaaa taaataaagt gtagccggag aacgccgttt
    24541 cttcgcaaga aaaaaaatgc agagcttcag cgagctccga ctacattgca agtgaaaatt
    24601 tataacgaaa aaagagagct acaaaagaga aaaattccat aggaacgata gatattagag
    24661 aagggtttca actcggagaa gaaaagacaa attttgtatg tggtttgctg aattctgatt
    24721 taccataaat agaggaaatt tatttctcgg agttctgcaa actctttggc aaataggaac
    24781 ttgccaaccc atttttgctc aactatatta tttgggataa actataacaa tctgtgagag
    24841 atttgcacta tctaagttat tgttcccagc aagatactta gtgactattc atcctccacc
    24901 caacctccac ccaaccttaa ggatgggttg atgttgacaa gcaagcaaga aaaaacaaaa
    24961 tcccttgacg aaacaggcag ttccaaaagt aatgcaacga ctttactttt caatatattg
    25021 gagcgataaa tgctaaaatt aggtcttaat caggaaatgg tatccagagc tcggaaagct
    25081 gcatttcaga taacaaatgc ttttacttgg ctgtttgagg gctatactac tgtagccaca
    25141 gaaaggacag tattacgatt gatgggaata gatggtgccc taaaagacgg taaacccttg
    25201 cccaatgttg tggtagatca tttggtggaa aattctgctc ttcagaaagg agcagcaatt
    25261 tgggtggcta atgctatgtt aaaaaagggt ttaacagcac agcaagtagc ggaagaagtt
    25321 gcctataaag gtttagatat aactcaaatt cctcttgcct caggcgaagc aattttggaa
    25381 actcttaatc ctttggtaga taaaatgttg ggtcatataa cagcccaaaa acaaaagcgg
    25441 gaggaacttc tggctaaata tcagccccgc ccccaaccag ttatctatgt aattgtcgct
    25501 actggaaaca tttatgaaga tgtagtccaa gccaaagctg cggcaagaga aggtgccgat
    25561 gctattgcag ttatccgttc aacagctcaa tccttattgg attatgtgcc ttttggtgcc
    25621 actacggttg gttttggagg cacttttgcc acgcaggaaa attttaaaat aatgcgtaaa
    25681 gcgctggatg aggtagctga agaagagaac cgttatattt acttaacaaa ctatgccagt
    25741 ggactttgta tgcctgaaat tgcagctatg gcggcaatgg aacgcttgga tatgatgctt
    25801 aatgatgcaa tgtatggcat tttgttcaga gatattaacc ccaaaagaac cttgctggat
    25861 caatattttg cgcgaatgat caatgcctat gcgggaattg aaattcagac cggtgaagat
    25921 aattatttaa ctacttccga tgctgtagaa aaggcatata cggttactgc gagccagtta
    25981 atcaatgaaa gttttgcttt aatgagcggt attaaacccg aaaaaatggg attaggacat
    26041 gcttttgaga ttgatcccga gctggaaaac agctttagtt ttgagttagc acacgctctt
    26101 ttaaccaaaa ccctctttcc tgatgcgcct gtaaaatata tgccaccgac aaaatttgcc
    26161 agcggtaata tttttaagac ctatctgctc aatgcgatgt tcaattttat cgggcaatta
    26221 acagggcaag gtgttcagct tttaggtatg atgacagagg caattcatac tccgcatttg
    26281 gcagaccgct ttctggctat tgaaaatgcc aaatatatat ttaaagcggt aaaagacctg
    26341 aaccaaaatt tatgtttaag cgaagatagt tttattaaca aaagagccaa tgaggtcttg
    26401 gctcaggcaa cggaattttt ggaaaaagta gctcgagaag gtctttttag tgctatggct
    26461 aaaggtgaat ttgccgatat taaacgaccc gaaaatgggg gaaaaggttt ggagggtgta
    26521 attatgaaag ataaggacta ttacaatccc tttttggaaa ttatgaaaca ggagcttgct
    26581 ttatgagcaa aaatataatt cgtccttatg gcgatacctt aaacgatgga aaaatgcagc
    26641 tttcttttac attgcccgtt tcttgtgatg ctttgggcaa agaagcagcc agaaaattat
    26701 tgttacaaac aggttttgac gaagcggaaa taacatctat gagcgattta acggagggct
    26761 ttacacattt tattgcctat gctgtcactt ccacaagtgt agatatcagc aaaatcaaag
    26821 taaaagtagt agaaacaccg gtaatgacaa tggaagaaac agatgctttt gtggcagaac
    26881 atttaggacg gaaaataact gttgtcggtg cttgcataga aagtgacgct catactgtcg
    26941 gaattgatgc cataatgaat atgaagggtt acaatcatcg ttacggatta gaacgctatc
    27001 acagtttcaa tgctatcaat atgggagctc aggttccttg tgaagaactt cttgccaaag
    27061 ccagagaagt gaatgcagat gctattttgg tttcccaggt agttacccag aaaaatatcc
    27121 atatcaaaaa tttaacccgc ctcatagaac ttgccgaagc agaaggatta agagataaaa
    27181 tgctgtttat ttgcggtgga ccgcgcattt cacacgaact ggcaatagaa cttggttacg
    27241 atgctggttt tggtcaagga acatatgcta ccgatgttgc cagctatatt gccattactc
    27301 tggcaaaaca aaaagcgcag gaataggcat ttataatgag ggcattagct taaatatatg
    27361 ttaaccttgc tggtaaccat aattgccttc gggctgatga tttttgtgca tgaactgggt
    27421 cactttttaa tggcgcgctc ctttaaagta ggtattgaaa gattttccat aggtttcggt
    27481 aaagcaattt ggacaactga gaaaaatggt attcaatatc gcctgggatg gattccttta
    27541 ggtggttatg taaaaatgca aggggaaaat ccggaagaag aaataagcgt tgataaagaa
    27601 aacacttttt taggcaaacc atggtggaaa agagctctta ttgccttcag cggacctttt
    27661 gccaatctgc ttttcggatt gttactattc ataattgcct ttatgctacc gcaaaaacag
    27721 gaagaccttg ttccggttat tcaaaatgcc aaaggaattt gggctgaagc attcagtcct
    27781 gcagacagca ttataagtgt aaacggcaaa ccgataaagg gttttcagga atttttagtg
    27841 tcgctttccg agaaaaaacc caataccatt tcctattttc ataacggaca aaagacagtt
    27901 ttagaagtag ctccctccca agtggattct ttaattaaat cccttgaacc caaagtagat
    27961 accacaattg gagaggtttt taccggaatg cctgcctgga gagcagggtt aaaacccgga
    28021 gataaagttc tggctgtaga tagtgtaaat gtttccaact ggtatgaaat gcgggaaaaa
    28081 attgtaggtt caaaaaacga tgaagttctg ttaaccattt tacgggacgg aaaaatcctg
    28141 caacgaaaaa tagctttgga agaaaatgtc tctatgggtg atcaaaaaat gattgggatt
    28201 agtcaatata tgccggtgaa aagtgttaac agatataatc ccctgcaggc aataagctac
    28261 ggcacccaaa gcaccatcag ctttattgtg atgaattatg tgggcttata caaattgatt
    28321 tcaaaaccgg aacagctgaa aaataatctt ggaggtcccg tgatgattgc tacaatgggt
    28381 caacaggttg ctcagcgcgg atttagttct ctaattatct ttcttgcttc cataagcttg
    28441 attttgatga taatgaacct tttgcccatt ccggttttgg atggaggaca tatctttttt
    28501 gcctttctgg aaggtatttt cgggaaacct gtaccgataa aagtgcaggc atttttacag
    28561 cgtgttggtt ttgctatttt actgttactg atgttttatg ccttctatgc cgatatttcc
    28621 aagctgctta tccgacaatt cttattaggg caataaatgt ttaatttcac tttacaaaag
    28681 accaacggaa aagccagagc cggaataatg aaaacagaac acggcgaaat tttaactcct
    28741 gtttttatgc ctgtaggaac tttgggaaca gtgaaggcaa tgagccccaa agaactggaa
    28801 gaagttcagg cacaaataat tttgggaaat acctatcatc tctatataag acccgggcac
    28861 actttaatcc aaaaagcggg tggtttgcat caatttatca gttggaatca tccaatctta
    28921 acagatagcg gtggttttca ggtaatgagc ttggctgctt tacgcaaaat aagcaaagaa
    28981 ggggtaaaat tccagtccca tattgatggc tctccccata tatttacacc ggaaaaagtg
    29041 attgaaattc aggaagctct tggcgcagat ataattatga gctttgatga atgccctccc
    29101 tatccttcta ccagaaagta tgtggaaaaa tcgcttgcta ctacattgga ctgggctgaa
    29161 agaggaaaaa aggcctttac gaattataaa catcaggcac ttttcggaat tgtgcagggg
    29221 ggaatttatg aggatttgcg tgaaaaatcc gcgttggaac taatggaaat ggattttgac
    29281 ggttatggaa ttggtggact tgccgtaggt gaagaaaaag aggatttatt aaggattact
    29341 tcctttctaa atgatatctt gccaattgat aagccacgct atttaatggg cgttggaact
    29401 ccaagcgatt tgctgaataa tatagaccgg ggcatagata tgtttgattg tgtgatgccc
    29461 actcggaatg cacgcaaagg cagtattttt accagatatg gcaaaatgat aattaaagca
    29521 gctcgctata aagacgattt cagccctatt gatcctctct gtgggtgtta tacctgcact
    29581 catttcagcc gtgcttatat caggcattta atcactatga atgaaatttt gggaatgcga
    29641 cttactacta ttcatagttt atggttctat caggaactga tgcaaatggc aagaaaagcg
    29701 atttgggaaa atagatggaa tgaatttctg gcagaaatgc ttcccgcgct ggacagaatt
    29761 gttcagtaag aaaacttttt taatatatct tggctttctt gctataatac ttgtaagagg
    29821 cacactcttt tgccaggaaa ccgaattcac ttctccagcc gattctatta aaataacggt
    29881 tcccgaacct tggacggaaa actggctgta taaaaaggtc tatcctctaa tttttaaaat
    29941 ggaggcagaa ctacagcagg aacaaaatct cttttccatt tttgagcaat atagcgggaa
    30001 aaagatagcc aaaatttaca ttgttaatct ggatgtgttt gaaatgcttc ctgataaacc
    30061 ggaaacacat atctataaca ggattttgga tttaggtaat tctcttcact ataaaacccg
    30121 ggattggatt attaaagata tgctgttttt tgccgaaggc gatgatttta atccggaaat
    30181 aatgaatcgt aaccttgcct atttgaagga tttaccctat ttaagagaag cagaactgct
    30241 gattagtgat aatgaagatg gcacagttga tgtttttgtc cttgtgcggg ataaattctc
    30301 tctggaactg agcggcagga ttatttccca aagtaaatac cgcttgaaga taaatgaaca
    30361 aaacattctg ggtttgggtt tgggactgaa acatatttgg catattaacc ccaaggaaat
    30421 gaagacacta agctgggaga catattacag cgattctaat atcaagaaca cttttttccg
    30481 cgttgatgct ttttggaagg aattttcagg taattcaaat caggacttgt atttaagcca
    30541 tccttttctt tatcctgcaa cgccacattc cggagggatt gaaggaacaa gaaattatat
    30601 tcaccctcct gctgatactt taactacgga aaaatggact cttggctcct ggtatgcgca
    30661 tagtttcggc ttttctgata atctgacgaa tgcctataaa tatgttgctt tcgggttgga
    30721 gaaaaactgg tttaccaaaa gtccgcaagt tgatgaaaat acaggtaaac cctggcagga
    30781 taatatcttt gccttaagtt cttttgcttt cacaaaaacc ggctacactt cctttagaaa
    30841 catcagttat tttctggtta acaatgtttt gcctgttggc tatttaactg aaacactttt
    30901 gggttatgaa ttcggagaat accataaccg tctttttacc ggtttgcatt attcccgggc
    30961 agataaactg cctcgcggtt catatcttta ttttgccagt gctttagaaa catatattag
    31021 cagtgaagga acggaacaaa gcgttttggc tgttgagccc ctttttattt cgcctttaca
    31081 acacatagga gatttccaaa gcaggtattt tttcaacagc aatttagttc tcggagggaa
    31141 acgctatccc agcgaacaaa tgaatgtttc ttccctttcc ggttatcatg gaatctggaa
    31201 tttacagggt gataaactgt tacgctctaa tttggaaaac gatattacta ctccttatca
    31261 aatctggggc tttaaaatag gactctttgc ctttttggat tgtgcctttg ctaccaatca
    31321 cttttcccaa ataagcacta aaaatgttct gctgacagaa ggagccggtt ttagaattca
    31381 taatcccgct ttaatttggg aatcaattga actctgtttt gctctgaatc aaaaaaaggg
    31441 gagtaaaggc gaatggaaaa ttacgctctc cacgaaaaaa ggactggacc ttcctgattt
    31501 ttcaggtaaa agaccacaga catataaatt tcaatgaaga atgaatacta tttagattaa
    31561 tagttttgca gtgattgttt gatagaaaca gcaactttct ggagagatca agttttccga
    31621 aggttttcca aaagggttct aacttgctgt tcagttaccg gtaaaggatt cagagccata
    31681 ttacgactgc tcataatttg aggaatagct ttatctataa agtcctcact gaagtcaatt
    31741 ttgcgttcaa aaggcaggga ctttagccag gtataaaagt catctttact tatttgcaaa
    31801 tagctgaaca ggtcatccag ttcttgctga acagcaggat aatagagctc cataatatct
    31861 tccattacca tagcatttgc caaaccatga ggaataccat aaaaagaggt taaaggataa
    31921 ccaatagaat gttgaagagt agtacttccc tgagctataa ccattcctcc gtaaagtgca
    31981 tctctcatca gttcttctct tgccaaataa ttttctggtt cggaaagaac aataggcaga
    32041 aacttcataa tagaagcaat acctttatag ataaagggat agacgattgg tgaacgctga
    32101 ttactataaa gcccttccaa gagatgactt aaagcatcca aggcagtatg cagagtcact
    32161 tttggtggca aagttaaagt atatttcggg tctaaaacag ctaaagtagg aaaagctaaa
    32221 tcacagccaa aaccggcttt ctttttagta agaggatcag aaagaacact atattgagta
    32281 acttctgttc cagttcctgc tgtaagggga attgccagaa tagaatatgc ctttttaaaa
    32341 acggaagtat tatacatttc atctctgttt aaggaatttg cagcagccaa ggaaattgcc
    32401 tttgcggaat caacaggact gccacctcct ataccgataa caaaatcaca gttattttgc
    32461 aaaaaagcgg atttgccttc cattacaata tccaaagtag gattttccgc aatgcggtca
    32521 aaaagagcat aactaatatc cgcttccctc aaaacagaaa tggtgtcgga taaagcgcca
    32581 ctttgtttag cgctgttttt tccggttaca attaaagctc tttttccgaa agaggcaata
    32641 tattcttttg ccttttgcat cgcattatcg gcaaaaataa tacgggttgg acaatagaac
    32701 atttagctcc tacttaagca gagtaatctt tttcgttata atgcctgcag gatgttgcaa
    32761 acgaagcaaa tagacaccgg aagccaaagc tctgcctttt tcatcgcttc cgttaaaata
    32821 gatgctatat ttcccttggg gcataagacc ttcggataaa gtaacaactt tctgtccctt
    32881 caggttgtat aataccagtt ctgcttttcc gccaacaggt aaagagaagg aaattgtagc
    32941 agtcggattg aaaggattgg gaaaagcgga aagagttgcc tgtggcacaa taatagtttc
    33001 atcggaattc ggtaaagcag gatttagagt aggtaagagc tcatagaaca tattagtaac
    33061 ctcatctaaa tgcataaaat acagcggaca gccaaagaga actaaagtgc cgttaaaatc
    33121 gtgcttgaaa gcaatacact gtcctgctcc attacttcct tccatcatat tggcaaaata
    33181 taaaggttct tcagctccac tgaaagtaaa aatatatggt aacatagaat tccaggagga
    33241 aagggtctta ttagtgtcca ctgttaaagt tggatagata ttggattgag cactgattaa
    33301 acaggcaaga ttatcgtaaa taggagttac tccacctcca aagcggttaa aaaattccgt
    33361 cgttaaaact gaaggggttt tccagccgga aataactatt tttcctccac ctgcaatata
    33421 actgctaagc aaagaagaat taccctgcag caaattttgg ttaaaatcat ccgaatgcca
    33481 aagcaccagt ttataatttc ccaaagtttc cagattggga agaccctgtg tagcacaatc
    33541 ccaattatca atcgggcagg aaaataaact taaagcggaa gcataaaaag tatcaaccat
    33601 ttcatcatcg ggattaataa gagttcctat tccgtctctg gtttcatcaa caactaaaag
    33661 ttcacccgta aaactgaatg cttgaggtgt aacggagacg gtctctgaag gtctggaaaa
    33721 atatccgttt acatcaacta ctctaaattg ataatcataa ctgataccag aaattgcagt
    33781 tgtatcggca tagctgaaag tgtcgttatt taaggtagca agtaaagacc aattttgtgc
    33841 actgctcaat ttacgataga cccgatactg atgatccttg tttggtataa atgtattcca
    33901 gaaaagtaaa acgcaattat tggtgggcag atattccgta aaatgaggaa tttgttccgc
    33961 taattccttc aacaaagtcc aatggttata attggaaaca atattgatat tatcgctcaa
    34021 aacggaataa ttaaaatcgt tggaatagcc atcaggagcg gcaattacca ataaagaatc
    34081 gctaattcca ttttgagtaa tccgaaaagg aatttccacc gtgaaggaag tggcagttgg
    34141 agaagtagtt ttggcatata tttccagatg gttattagtt tcattttgcc acagggaata
    34201 tttcacaccg ggaattccag aaccgtaaat ccattggttg aagaactgac taaggtcttg
    34261 tccactaact tgttctgcca gtgcttcaaa ttctgccgta atagcattcc catttttata
    34321 ggtattataa taaagccgaa gcaagttgaa aaaattggtg tctccaagct ttagccgtaa
    34381 catatgtaac acgctggctg ctttttcata agagggcggc gaaaaatagt tataaaaaga
    34441 agggttatat atagtttgag gtcctgaatt ggcttcccaa ttcagataaa actgatgaaa
    34501 attactatgt agataattac aggcactttg ccagccaaat cttttatctg tccagagaaa
    34561 ttctccgtaa gtagcaaaac cttcagaaag ccaaacatcc ttaaaagtta aaaaactgac
    34621 tgcattccca aaccattgat gaacaagttc atgagctata actgtttcat aagctccgtt
    34681 tccactaata atataatttc ctaaagtagt catagtttga tgttccatag ctccgtaagt
    34741 gctcatactt actacagcat tgccatattt ttcaaaagga taatcaccga aaagttcaga
    34801 aaaccaggaa atcatccaag ggaggttttg caggtctatt aaagcattat tgtattgatt
    34861 ttgcattacg aaattctgaa cgggcagatt attctgttcc ggaactgtct gatttatttc
    34921 tacataagga ccagcagtta tacaaaccag ataagtagtc atcgggtttt ctccaatcca
    34981 atgagttgta gaagtgccat caccattatt cactatttcc gttctaattc cattagcggc
    35041 aactttccag tcactgcgca tagtaatgtg caaatccaca attgctttat cccaggggtg
    35101 atcataacag ggccaccagg cacgagcagc atcaggatcg gaaattgtaa aaacggtatt
    35161 attactaaaa atcattccga tattatagta actataaact aattgaggag tacctgaata
    35221 aaaaacctgc gttgtaaatt gctctcctgc agatacatta accggaaaag taaaataagt
    35281 gccatagtta ataattggtg tatgaattcc gtttaccaaa acattactca cattgaaacc
    35341 ggttaattcg taagacatac tggtaagatt ttccgtggca ataaccgttg ctaatacatt
    35401 accccaaata taatgtaaag cgtcattgat agttagcgta atttcatatt ttagcacatc
    35461 aaaaccgtga gcagaatcgg cacggcaaat ttcttcttcc agatatgcgt tttgaggcat
    35521 aggagaacta tatttatggc caggtttcac attttcaatt gtttgactaa acaacacccc
    35581 tatgctgata aataagccaa gagttagaag aactttttta acctgcaatc tcataataac
    35641 ctctgaatta aaaaaatgtt atattaactt atacaagcat atttggttcc aaattttata
    35701 aaataaatgc cggtatacta tatatcaatt ccgatttttg aaattcgcat ttattaactt
    35761 ttgcggacga atcatcctct tcaattcata tcttatttca tctttatcca ttattttgct
    35821 tgacatcttc tctctccttt atttcttgtc tataatgaaa gacaatgtaa gtaatattag
    35881 ttagatggtc aaaaaaagta acttatttta gttatggtgg taatatatgg attatatacc
    35941 tttattagaa gagctgttaa attctcttac gataagcaaa aaaagattgc agaaactatt
    36001 agcatttctt agggaaatgc ctttatccca aaactcgcac aactttatcc ttaatcctca
    36061 atgatgatta taaatagatt atctatactc aagatgaaga aaaaattcat atactttcca
    36121 ttattcacat tataaactga cacccaaagt tgcaagtagt gacacaaggg gaaagtgggg
    36181 ataaggcttt gatgggtggc gagatacgat ggactggctt tggaaatgtc caagcgcttg
    36241 tttggacgtt tatggactgt ttgctgacac tattactgca aattagcgag gcgtttggac
    36301 aggctttgga aagagcagtt ggaaaatcgg aactttgaaa ttagaaaaag cgcaattgga
    36361 gtgcgattgt gccgatcagg aaagctccgt aattccaatc tgagtctaaa accagaaccg
    36421 ggtagtctat attaaatggt tggaggataa tctgttgcct gtgatggtcg atttgcacct
    36481 tcttgatggt gagaccatcg tcagtcctta caactgcaat ttttccatcc gcgttttccc
    36541 agctctgatc gctcttgatg accacgatat cctgatgctg gattacaggc tccatgctct
    36601 gcccatttac ttgaaagaca gtatattgct tggggctgcc aggcaggaat gagactggta
    36661 actgaatctg accaacctga ctccactcgt ctctgatctc catgggaggt cctgcagcta
    36721 tctcacccac tatgggaaaa gtgatagatc tagtgggatc gaagtcctga ttatctactg
    36781 gatgaaggat tggagagatg atggctggat ttgatttatc ggaactggtt gagcttattg
    36841 gtacttgatt agactggatc agctcttgga actcatcctt ggcatacatg gagccgtttc
    36901 cgttaaagag ccagtggaga ttgatctgat ctctggagag ggcttccagg aactcaggat
    36961 cgggatagcg ctcgttcagc ttgtacctgg agagggaagc acgggagatg ccgtacttat
    37021 tggcaaactg gtaatccttg agtcgcatcg ccttgatcac caagctaagg cgctgtccga
    37081 tagtcggttt attcatcgta aactccatac agaaactttg ccattgacta tatccttatg
    37141 gctgcaagat tggtttctga tgccattctg ctgcagggta gtttggagtc aatactaaag
    37201 tatatattgt gacccaggtg gattataaag tattgccgga gaatgcgtaa gaaagctggc
    37261 aaactcgcag aaaggacttc ccaaggttct gcgcttgtgg ttgttagcac cctactatat
    37321 acataagaga ggtgtagaat gactaccaag aaacgctgcc aatcggaagt gcagaaacgg
    37381 gcgaatcagg gcaatgtcca agcgggtacc cctgtccaag cgggggtggg aatgaggctt
    37441 aactcattga taactatgat gagaggccaa aatgtccaag cgcttgaccg catttttcag
    37501 gatttcagac tgaaatgtcc aagtgggtcg cagtttttga aggatttgga catggacatt
    37561 caagtcgatt tgatctggtt acccatcgat agggtggcct tactgctgaa caagaacctg
    37621 aagacaatca gaaggatggt cgatgccggg aagctgactg cgctcaagca tcagataccc
    37681 accaagaacg gggctacgat caagaccttt gtacttgcaa ccgaagacat catccaagct
    37741 gagtatgatc ggttcagaga cagcctgcgt aaacctgagc tctattttga ggaagtgatc
    37801 gaattgggag tccacagcta tcacagtgtc ttcctggtct attacgagaa tggtaacgat
    37861 ctggttgcta aggcaagcga taaatcagga ggttcagatg tttccctata gtggcagacc
    37921 gactgaggag ttccttagag aatatcgcag caagctgaac gaactgcaag agctgatgga
    37981 aagtcaggga ctgaagctgc agatcaagga tgggggcaga tgcatagtgc tcccggaggt
    38041 taagtctgga atgggtaagg ttagtgggaa aagtaaagat gatggtgatg ggattagcaa
    38101 tagtacaaac actgcatctg aaaccgacaa tagcttcatg ctctcctaca gccagcagta
    38161 taatgccaaa gccggtactt cagaagacaa tttggagtcc gagacggtct atgagaccta
    38221 tgatgactat atggagttcg aacgcaagct cgctctgatc catgaagcca gagataatgg
    38281 ggtagtgatc gacttctcca gtgactttct cgatctggct ccggtggaag aggaaaggct
    38341 gttatacaag gatgaggctc aactctatgg caggttctgc caggaagtga tccgcagact
    38401 gtatggagca gaatccaaga tcctggcctg gaaacgaatc accagtgact ataactgcaa
    38461 gcgactggtt cctgaactgt ataacctcaa agggcccaga aaagagagag cgctacggga
    38521 atggctacgg gtctaccatg agacgaatct ggatatgtat gctttgatcc acaagagcaa
    38581 gggtaagtac aaagaacgca agatcagcta tcaggaacag caatacctac tgcacctgct
    38641 gctcaatccc aatcgcatca aggtcggctc tgccatcacc aacctcaagc agatgagcag
    38701 aaacggagtc attgaatccc agacaaatga acgaactctg agacgctggt gcgaggaatg
    38761 ggctgcccgg catccggcag agtggcatca agccagagat ggcagcaagt ttgtctctga
    38821 gaatgtcatc aagagcatta tgagagatga tagcactctg caagtaggac aggtctgggt
    38881 ggcggatgga cataccctct ccttcgatat cctcaatcct cagaccggaa gagcccaacg
    38941 tatgaccatg atcatggtga aggactgggc aagcagatat ccggtgggag cctcactagc
    39001 ctttactgaa gatagccagc atatcctcac agccttccgt aacggcttca tcaactgcgc
    39061 tcagatcgca gataacaaca gtggcaccat ggcagtgctg cccaagtatg tttatctgga
    39121 taacggcaag gctttccgag ccaagctatt tcatgatagg tgggaagagc atgatttgaa
    39181 caaggaactg ggcggtatct ttccccggct caatatcggc gtcaggttcg cggagagtta
    39241 caatgccaaa gccaagatca tcgaacgctt cttcaagacc ttccaggagc agtttgagcg
    39301 gttcatcacc accttcagag gagcctcgat tgacgataag ccctcagtcc tgcacagaaa
    39361 cgaggtctgg gcaaagaagc tatatgccgg gaaaccacct accattgaag agacgatgca
    39421 aatgatcgct ttctatgtac gcttctgcta tggtgagaca ccacatacct cactcaatag
    39481 acggactcct tacgaggtat tcagtgcagc caggatagtc gagagccggc agattgagat
    39541 ctccaggctg aacttcctga tgatggcgat ggagcgcaag aacatcagag ctgaaggcat
    39601 caggttggat aagaaggtct actggcatcg ggaactggtg aatcatgtgg gacagccctg
    39661 catcatcagg ttcgattaca gcgatgcaag gtggatagtg gtctatgaca agaacgacgt
    39721 gttcatctgc caggcagcac tccgaaagac ccagcatccg tttatcgagg tctcgatgga
    39781 tcaggcagtc tcgcataagg aactgaataa agaatacaaa gagatcaagg gcctcagacg
    39841 agagaaagag cgtagcgcca agaagtgggt ggtgaactca cagagagcag ttgatgccct
    39901 gcttaagaac tcccatgcca agcaggagaa gcttgaggat gaaggagcca gagcgctgtt
    39961 cagtagtcct cccatgttgg aagcacctcc caaggatggc gatgagataa ttgaggagct
    40021 gactagaaag atgatgatga gactgccgca gcatcccgat atgctgagtc cggaagagcg
    40081 ggaacagctt gatcccaagg agaaagagag actggcagag caggagaggg ataggataat
    40141 cctaaaggaa attaaagcac aattcgaccg catcggactc gaatacaagc ccggtaatga
    40201 taaaccatcc ttggagtttg cgataggctt cagtcaaacg gctagagagt actgcaaagc
    40261 tcaacaacaa gcccaaaacg aacaagaagc atcagatgaa gatgaagcta ccactgccac
    40321 agcaacaact tatgttccag gcaaggaagc aggagatcac gatccagcgc aaacgatcat
    40381 caacccagca gaattgcctg atgatgagca agctcaggat gaggccaaga acgggcagtc
    40441 acctttcgca cacctctcaa gagcagaact cctcaaacgc atcgggataa actaatagga
    40501 ggatatcatg aaacagggaa aactggtcag gacgcagaac gttatagctg cagacaaatg
    40561 catcgaatac ctgctaaccc gacccaagct ggagatggtc ggacttgggc tcctatatgg
    40621 cagaccagga cttggcaaga ccacctatgc cagccggata gccttcagtc agggttatct
    40681 ctatctcagg ctggaggcaa ccaccacccc caaggccttt gcatccaagc tgctattagc
    40741 cctatacaag agatttggca tgggagagta cattccttac ggaacagcaa atgacctctt
    40801 catgatgtgt ataacggttc tggaggatca caaggatacg atcatcgtgg tggatgagat
    40861 agactatgcc ttccggcatc cccaactctt gggagcgatc agggatatag tggatgtgac
    40921 cacagcgata gtgatcctgg tcggtatgca gaatgccaag gaccgactaa atcagatcaa
    40981 tgagtattac ttcgacagat gcaacttctt ctatgagttc ctggacctgc caaagaagga
    41041 catcgcccag atatgcagtg aggtcttgga gatcaagttc cagccggata ttgtggacta
    41101 tgtgtacttc cactcctgcg gtaatgtcag aaaagccatg aagctgatcc gttcagttga
    41161 agagataggc agatacaaga acctcgaagt agtctcgcaa gccgaccttg atgattgagg
    41221 tcagaatgac ggacaaagaa ctcatcctca acttcatcaa tcagtatgat ctcctgttca
    41281 atgcagaact tatagccaag ctgaccagtg taagcagaga agcaatagaa aagctactac
    41341 ccgatctgct ccaaagccag gcgatcaagc agatagaaga tagtccaccc atctacgtgc
    41401 gagtcaaccg ctaccaagcc aggatcggct atcagcacta caagggctgg acttttagca
    41461 tagcggacgc ccacaagctt ctggacatcc tggaacaagg tagatacaaa tccatccgag
    41521 acatcgctca agccatcggc aagtccaggc agtgggtcta catctatctg gaagcactgg
    41581 catcgattga ggttgttgat ctaaggcagc acatttatgt ggtcatatcc cgccaaaacg
    41641 ttcccaaaat cggtagaaaa gtccagaaag gtatattggg tcaactgcgg agtctgaata
    41701 gggctgggtg ttatcgatta atagaatgaa aagtccgtaa ttgcggctat gttattagaa
    41761 cgatatcagg ggcgtgaatc tctatgtttt cccttgtgtt caccacatag ttataagtat
    41821 ttcccatgat aaatcagaca tggctaggat gattcttcaa tcctatgcaa atcttactaa
    41881 ttgattccaa gtcaatacga taaatatcat ctcaaacagc agcataatga ccagatcgaa
    41941 aattctcatt gacatttatt ctgctctctt aaaatgagca tatgctcttt tgagtatagt
    42001 tgttttcaag gcattaagtg ctgataagca tactcttgcc atagtttata agcggtccaa
    42061 aagtcagctt ttataagtat ttatttagtt agagtccagt gactcaaata taaggagaaa
    42121 gtaatggata agaaaatcgc cataccaaca accaacaaag tactgagtgc tcattttggg
    42181 cattgtgaga aattcgttat ctataacgtt gtagatagta aaattgttaa tgacgaatgg
    42241 attacacctc caccccacga acccggttca caccctaagt ttctgagaga aatgggttgt
    42301 gaaactatta ttgccggagg tatgggcgaa agagcacaga ccctttttgc tgaaaacaat
    42361 atagaagtat ttattggagt tcctgatatc cctttgactg agcttgtgca gatgtacatc
    42421 aagtcagaac taaaatcagg agccaatagc tgtgaccatt aaggtgttct caaaatgaaa
    42481 ttgaccattg ccagtggaaa gggtggaaca ggtaagacga ccttttccac aaatctcgcc
    42541 atgtatctgg cagagcagta taaagtcgtg cttactgacc tggatgtgga agaacctaat
    42601 tcgggattgt tcgttcatgg acggcaaacc catcagcagg atatgtttaa aatgattcct
    42661 gaatgggata aaaacgtatg tacgctatgt ggaaattgtc agcagctctg ctcttatcat
    42721 gctgtaatga aattgggcag ccaggtaatg gtttttcctg agttatgcca tggttgctat
    42781 gcctgcagtg aactatgccc ggcacatgcc ttaccgatgc accagataaa aatgggtgaa
    42841 ctcagacaat acagagcagg caatctcgac tttatagata gccggcttga cgttggtcaa
    42901 gaacaggcag taccgttgat agcacagacg ttggattatg tggaacggaa ttttgatact
    42961 gcttacctca aactatatga tgcgcctccg ggaacaacgt gtccggtcat caaagcaact
    43021 caggatgcag atttagtgat actcatcaca gaaccgaccc cctttggact ccacgacctc
    43081 aaattggcaa ttgaaaccat gagagagttg gagaaagact ttgtagtagt agtcaatcgc
    43141 tttggcatcg gcaatgatga tgtcctccgg tactgtgaaa aagaaggcat catagtgctg
    43201 gcaaagcttc ccaatgacag acatatagca gagctatatt ctcgcggtga acttgtttac
    43261 cctcatattc cggaattcgc agaacaactg cgattaatag gtgatttcat tatgagccaa
    43321 caagggatgc aatcatgaaa gagattgtag taatttcagg taaaggcgga acaggtaaaa
    43381 cttccattac agcatctttt gccatgttgg gtggcaagga tatcgtagtg gcagattgtg
    43441 atgtcgatgc tgccgatatg catttattga tgaaaccaaa cgttaaaaca gaagaagact
    43501 tttatagcgg aaatgcggct gtgattgaca gagcaaaatg cctaaattgc ggcaaatgcg
    43561 cttctgtatg tcgttttgat gctatcaatt tgattgaata tctctattca gtttctgctc
    43621 tcaactgcga aggatgtggg tactgtgccc gtgtttgccc ggttaaggta atctctatgg
    43681 tcgaacaaaa tgtaggcaaa ctgtatattt ctgccaccaa agcagacaat atgatggtgc
    43741 atgccaggtt gctgagcgga gcagataact ccgggaaact ggtcgcccga gttaaaaaag
    43801 aagctaagca gatagctcag gaaacaggaa aaggatttgt gattgtggat ggttctccgg
    43861 gaatcggctg tccggtgatt tcatccttat ccggagcaga ctttgtggtt ttggtcactg
    43921 agcctaccgt ctctggcttg catgatttga agcgtgttta tgaactcgtg aatcgcttcc
    43981 agatatctgc cggttgcata atcaacaaag cagatattaa ccaggatgtc gtcaatcaca
    44041 tcgaaatcta tctaagagag aacaacatcg tccacttaac cgatttaccg tatgatgagt
    44101 cttttacatc agctatgact ataggacaaa ccatagtgga atatgaccat gataagcttg
    44161 cagatctgct gacagtaagc tggaataaga ttaaacagat attatcaaag gagcaacaat
    44221 gaaaatagca tttacagcta aaggcacagg atgggattct ccgatagacc ctcgcctggg
    44281 cagaacagaa tacatcctgc tttacgatga agataaagat gaactttctg tcacagataa
    44341 tagagaaatt gaaggagtag cgcatggtgc tggtcctaag actgcacagt tactgtttga
    44401 tataaatccg gatgtcctaa taacggggaa tggacccggt gataatgcag cagttgtatt
    44461 gaagcaaatc ccgatgaaaa tatacgttgg ggcaggcgaa ttgactgtca aaaccgcact
    44521 tgaagcttac aaaaataacg agctgaagga attctaaagt ggtcagtttt gatgtcatca
    44581 ttattggagg aggaccttct gctatcataa ccgggcttac tggtaaaaaa cagaatccgg
    44641 atatgagttt tcttatgatt aaagaggaag agaagggatt ggtcccttgt ggaattcctt
    44701 atatcttcca tgatttgggg gatgtttcca aaaacatgat gggtccagcc ccatttgtag
    44761 cagcaggtgg tgaggttctg attgacacag tgacagaagt tagctttaag gcaaagacac
    44821 tgcaaactaa atcagggtta acatttgcct atgataaact ggtctttgcc acaggttcgg
    44881 ttccgatagt tcccaccttt atcaaaggtt atgatttgga gggtgtagag tacatccgga
    44941 aaagctatga gtacattaaa gccctcaaac aaaagactga tttggcaaag aatatcgtga
    45001 ttatcggggg tggcttcatt ggtgtggagg tagcagagca gcttgccaaa ttcacagaca
    45061 aaaaagtttc ccttatagaa atgcaggaat actgcttgat caatgcattt tctcccagac
    45121 tgacaaaact ggctgatact gccgttcggg agcagggagt caatctttat acttccgcca
    45181 aggtgactga aataatcggt caagctggtc atgtggctgg cgttaaactc gctgatggca
    45241 cagaaatccc ggcagaactg gttattcaaa ctatcggata tactcccaat acaatgcttg
    45301 caggtcaatc aggattagaa atgaactcaa aattggctat tctgcgtgac aactacatga
    45361 gaactgctgt gaaggatgta tttgctgtag gagattgctc agaatctatc ggctttgtca
    45421 caggtaggcc ggataatgtg atgctggctt ccacagccac agccgaggct cgcatattgg
    45481 gctacaatct gtttaaaata ggtttgatac gcactttttc aggcacactt tccgtatttt
    45541 ctactcaaat cggaagcaag gtatttgctt cctgcggtgc aattgaacaa actgctgaag
    45601 ctgcagggat tgaatatatc agcggagaat ttgaggattg ggacagacat ccagagagtc
    45661 tatcagatgc ttcaaagtta tggataagtt tggttgtatc tcccaaaaca ggggttataa
    45721 tcggtgggga gatttatggt ggaaaatcag caggtgaaat cataaacatc atcagtctgg
    45781 caattcagaa aggagtgact gtttacgagc tggtatctta tcaaataggc acacacccgc
    45841 ttctaacaac tgcacccact aagtacgtac tcatcaaagc tgcggaattt gctatgagca
    45901 aaatcaaact ttttggtaat tagcccaata acctgaggag tattatgcca cgcgtaaaat
    45961 tacttcgtat gctggatgat ttgcctatca tcaaaggctt tcgtcctatc tggatgaaag
    46021 ccaatttacg tcaggctgtc atcatgaatc tggaagagta tgaagcattg cgtctcatcg
    46081 attatgaaaa acagatacat gaacaagctg ccagcactat gaatatatcc cgacctactt
    46141 ttacacgtat atatgaaact gccaggcaga aattgaccac agcattggta gaaggacgca
    46201 gtttactaat tgaaggtgga aatgttacca tccaagagtc gcattggtat tgtgagagct
    46261 gttttcacaa gttttccact gccatcgctt ctgaggcaga tgaaatggcc tgtcccaaat
    46321 gtaattccaa acaattatta aacttgaacg actgctttct aagcggttgt cgcagatgcc
    46381 gcagatgcag ataatagaaa acggcttaaa caacaaacca taaaaaaacc ttataggagg
    46441 ctaaaatgcc acggcatgac ggaacaggac ctgccggtga tggccgtccc ggaagaggga
    46501 tggggccatg ccaaaggttt ggaagcaatg taaacaataa cagaagcaca ggatatactg
    46561 ctgttaacat cttaatagac atgataaaat catttctagc aaaaaaacca atacgacaag
    46621 gaggtaaaaa tgccgtatca tgatggaaca ggacccttcg gcgatggcag gcccggaaga
    46681 ggattaggac catgcggaag gtttggagcc ccggtagggg gaggatttgg cagaggttat
    46741 ggccgcagat tgggacgcgg ttttggcgga ggttatgggc gcggattcgg ttttcgcaat
    46801 tatggatatt acagggatat tccgggttat tacaatcctg aagccaaaca ggacatatat
    46861 ccctataccc gtgaggacct agaggctcaa aaggatgaac tggaaaagca gattaagtgg
    46921 gttaacgaga gaatagctga aacagaaaag tagaatcagc tttttcagat aaaacccgct
    46981 atagaaaatt agctaatcta ctttcaaacc ttgcagggga ttattatgcc aaatcgtgac
    47041 ggaacaggaa ttgctggttc gggtcgtccc ggaagaggaa tgggaccttg caatcgcgca
    47101 gtcagtgata aaaaggtttg ggaaaaagca cttcgtaaag gaactaaaaa aagataatgt
    47161 agaagatgtt cctacaaaga agacaccaac acaaaaggaa aagagttgat tagttcaaca
    47221 ttagaacata gcgtctcgtc aatcctcaac agtcttagta atcttttttt gaattttaag
    47281 taaagaaaag ttacaggatt ttgtcaagtt tgacgggaca ctatcaatta caatgatatc
    47341 tgaaaaagaa agggaatcaa aggcatattg aatggaagat aacgaaaaaa aagagcaaag
    47401 tctaaaagcg aatctgcaga aaatcaaaca tcgtatcgtg gtcatgagtg gcaaaggtgg
    47461 agtcggtaaa agcttcatcg ctgtcaactt ggcttatggc ttagccttgc agggtaaaaa
    47521 agttggtctg ctggatgtgg atatccacgg accttccctt gccaagctga tgaatattga
    47581 aggaattccc ttccccatca accaagccgg agatatgccg atgccggtaa aagtactatc
    47641 taatctaagt gttctgaccg tagctactat gctgcaggca gaggacaatg ccctcatctg
    47701 gcgcggaccg ctcaagatgg ctttgataaa acagttcttc actgattttg agtgggaaga
    47761 gctggattat cttattgtag attgtcctcc cggtaccggc gatgaaccac tttcggtaat
    47821 ccagacttta gagcatgtgg atggcacagt aatggttaca actcctcagg atgtcgcatt
    47881 aattgatgtg aaaaaaagtg tctccttttc tgagaaaatg aacgtcccta ttctcggaat
    47941 aatagagaat atgaagtatt ttgtctgccc gcattgccag aaaccgacca atatttttgc
    48001 tggcaacaag atagacgaac ttatctttga gaagagcata gatcttcttg ctgaattaga
    48061 gctagaccca aatatcggga tagcaagtga caagggcaga ccatatatct atgattataa
    48121 taaactttcc aatgctgaaa agctcatgga agctgtgctt aaaatcatcg ctaaggttga
    48181 aatagcctaa gtggaatatt tgtttggtcc tgtcccctca cgcagattgg gagtttctct
    48241 cggtgtggac attgtccctc ataaagtctg cagcttaaac tgcgtttatt gtgaagtgga
    48301 caggacaaca aaccttacca taaaaagaga ggaatatgtt ccgatagacg ctgtcatcaa
    48361 agaacttgaa ggttaccttt cccaaaaacc agaactggac tttgtcactt tctccggtca
    48421 gggagaacca accctcaata gtggattagg caaggtgata gatttcatta aggacaattt
    48481 ctctctgtat aaagttgcgg ttatcactaa tgggactctt ttctgggaca aagatgtaag
    48541 agatggggtc aataaagccg atgttctatt gccatctttg gatgcagttt caccgataac
    48601 ttttttgaag ctcaaccgtc caagtaatga gttgcacata gatcgaatta ttgaaggact
    48661 tatcaagtta agacaggagt tcagggggaa aatttatctg gaggttttct tagttccggg
    48721 cttgaatgac acagaatcag agctagttct actaaagaat gccataaaaa agatcaaacc
    48781 ggatttagtt cagttaaaca ctttagacag acctggaact gagctatggg taacacctct
    48841 ttctcaaaag aagctaaggg atatattggt atttctcaaa ccgctgcatg tagaaatcat
    48901 ctccaaaccc caaaccagaa agcagataca aagtttcaat gagaatatct cagacggaat
    48961 tctggaaacc ataaaaagaa gaccagctac agataaagat ctgtgcaaaa tcctgaatct
    49021 gaacctaaat gaactgaata agtacttgag cgacttactc gaaagtggag aaatcgaaac
    49081 agaagagatg aacagaggtg tgtttttcaa aataaaacct cgcaataagt aaatgaaact
    49141 tctaatatta tatgtaatga acagtactta aataaccgaa ggagaaaaaa tggaacaaac
    49201 aagcatcaat taagggatcc cattattggg agacaaattc ccggacatga aagtccagac
    49261 cacccggggc gagataactc tgcccggcga tatgaaagga aaatggttcg tcctatttag
    49321 ccatcccgcc gattttacac cggtatgcac gactgaattt gtagccttcc aaaagcgtta
    49381 tgaaaaattc cgagagctca actgtgaact tatcggattg agtgttgacc aagtctttgc
    49441 ccatatcaaa tgggaagaat ggatcaatga aaacttggat gtcgaaatcg aatttcccat
    49501 catcgctgat acgggtgcag tcgccaacaa actcgggctt attcatcccg ggaaaggcac
    49561 caacacagtc agggcagtat ttattatgga tgcagagggt attatccgta ttatgctata
    49621 ttacccccaa gaattgggta ggaacatgga tgaaatactg cgagcagtaa aagcgatgca
    49681 gattgctgat aaatatgggg ttgccatgcc tgctaactgg ccgaacaatg aaatcatgaa
    49741 tgaccatgta atcattcccc ccgcaaagga tatcaaaacc gccaaagaac gcatggagaa
    49801 agccaaaact ggtgaatatg aatgttatga ctggtggctt tgccataaaa aactgtaaaa
    49861 tgcataataa aagaccgttg aataatctat aacatcggat tcatcctgtc ggagtgccgg
    49921 atttatccgg cactccgcag gtcggactat acatcataaa taataaccat gcatgcaaat
    49981 agtcattagt caagcaccga ctgaagtcag tgcagcgacc ggttgaaacc ggtgataccg
    50041 ctatgcgcaa cggtcttaat aaaggtattc taaattagaa ttaagaggtt ataaaatgac
    50101 agataaaaaa gtaaccgtat tcagtactcc gacctgttct tggtgtgtaa gggtcaaaga
    50161 ttacttgaag aaaaatggcg tctcgttcag tgatttagat gtttcaaaag attacaaagc
    50221 agcccaggat atgattcgta aaaccgggca gcagggtgtc ccccagattt gggtgggaag
    50281 ttcagctgta gtaggttttg ataaaacaaa actagatagc atactggaac taaattaaac
    50341 gggagcctct atcgttcgct tgtaagtcaa acggctatct ccaccttgct aagcgaatgc
    50401 agaacccata acaactcaaa acaaagcagg gaaagtaaat gcaatattct caaaaggtca
    50461 tagaccactt tatgcaccca cataacgtag gaaagatgga aaatccggat gctaccgcaa
    50521 ccgaaggtag tcccgcatgc ggagatcagg tgaccatgta cctaaaaatt gagccggata
    50581 aacatacgat cgaagatata agcttccttt cttacggttg cgcatcaaac attgccactg
    50641 cttcgattat cacagaccta gccaagggca agactttgca agaagctaaa aaaatcactt
    50701 ggcatgatgc tgtggaagcc ttggatgggc tgcctcccga gaaaactcac tgttcagttc
    50761 tggcttctga taccctccag gctgcaatct caaactacga gatcactcat ggatttaagg
    50821 aagttgttaa ttttaataag gacactatac ttgaggaatt ggaaaaggtt atctatccgc
    50881 aggtcggaga ggacacgttt ccctcaagct ggtgaaaaac attgatttca tcgatggaga
    50941 agtgctgatt gatcttgata ttttaaactt cgatcaatgg cacgagaaca tcttgcagga
    51001 gattcgagaa catcttgaga agtatcctga agtcaagaat atccagataa aaaatccttc
    51061 attacggctt tgaggctacc gcacagagac cctaataatc ataacttcta aataataaaa
    51121 agtaaagagg tatctaaatg aagaaagacg ctaaattcaa caccaaactc atccacgccg
    51181 gtcacaaacc ggaaccctat cattcagtta ttactcccat ttatcaaaca tccacttttt
    51241 cctttgaatc cgcagagcag ggagaagatt gctttgccgg agatagtgac ggttatattt
    51301 ataccaggct gggcaatcct accacccgtg ctttagagga tgcagttgcc agtctggaag
    51361 gcggagtagg tggtattgcg gttgccagtg gtatggcggc ggttacaaca atctatatgg
    51421 cttatctggg tgctggcagc catatcgtct cttctgcggc tgtctatgga ccttccagag
    51481 tagttttaga gaagcatttt tcccgctttg gagtagaatc cacttttgta gatacttctc
    51541 taatagactt ggtcgctgct gcaatcaaac ccaataccaa actgatctac atcgagacac
    51601 ccgccaaccc gaccattgcc atcacagata tagtttcttg tgccgaactt gcccacaaac
    51661 atggtatttt attaattgta gacaacactt tctgttcgcc atatttacag cgtccattgg
    51721 aactgggagc agatgttgtg ctgcactcca ctactaagtt catcaatggt catgctgatg
    51781 tagtgggtgg tgtaattgta gctaaaacac cggaagctca taagttcctg tatagcgtca
    51841 tgaccaacat gggcggtaat atggacccgc atcaggcatt tctggtcagc agaggtttaa
    51901 agactttgtc catccgtatg gaaagagcac aggaaaacgc tatgaagctg gcagtttatc
    51961 tggaacagca tcccaaagta gcatgggtgt tgtatcctgg tttgacttcc catcctcaat
    52021 atgaacttgc taaaaaacag caagacggtc caggtgcaat gatatctttt ggattaaaag
    52081 gtgggctgga agcaggcaga actttgatga ataacgttaa attggctgcg ctcgcagtat
    52141 ccttgggcgg agtggaatct ttgatccaac acccggcttc aatgacccat gcgaaaatgt
    52201 cttatgtaga caagcagaaa gccggtatca cagatgatct ggttcgcttt tctgttggga
    52261 ttgaggatta tgaagatatt gtcgaagata tccagcaggc ttttacttat gtttgaatga
    52321 attaatagac atactcactc tcagaggatt ggctttatct gatatggctg atcctctttt
    52381 ttgtatgttg tatgcgatcc aggtaccaat tgcatttggt tcaagtatat ttcatcagtt
    52441 tatgatacca tttcccaaac ctgttttttg ctcaattgga ggccagcaaa catggatatg
    52501 atcaagacct gctttctaac atcaaaatat tgatttctag tgcatactgc gcattgtaag
    52561 caatcatcca aattggcact tgcagatatc atgtcagaaa ttgcaatttt cggtgatcga
    52621 aattgcagaa aacctgtcag ataataccta aattggcttt gttaaggatg agcaaggatg
    52681 cgctacgatg ctacagtttg tagatttcgc tgcacaattt gcagatttcg ttgtcacgtt
    52741 gcacacattc tcataggacc ttaaaccgaa agtaaggaga gtaaattttg cttgacaaaa
    52801 ctttagctat agataaagtg gaattagata acttggaaaa gacagtatgt atagatgatt
    52861 tattacagcc ggttgttcat ttaaggtgtc ctaaatacaa agtatctttg tttgtacggg
    52921 aaaaaatcca cgatgaatta atggattttt tgggtggaaa aggaataaac cctaaatttg
    52981 tacctggcga tataaattac agatacgata agttatcagc aatagcagcg gtgatttacc
    53041 agaaccggta tcgtgatgaa ctatacagaa aagagcaaaa taatcctgtg attgctgcta
    53101 tgaagtttat ggacaaaggg agaaatgaca ggttatattg cagggaaatt tactttgaag
    53161 aggaaaagca ggtaatcatt tgtgagatac atagtggtaa aaagaaccag gagaacagga
    53221 aaaaagaaaa aaatataata aaaaaggtgg cagggtatga gttcaaaata caatcatgaa
    53281 aagataaaac aatctatcca gggtaatctt cgcaggtatg aagcaattgc cttaagtatg
    53341 gtgataagct ttgaaattga ggattttatc aaggaacggg gaattagcaa acaagagctt
    53401 gctaaagctg caggggtttc accttcctat ttaagccagg tttttgctgg agatagattg
    53461 ctcaacctta ctatgcttgc tgggatatca caaaaatacc aggttaaatt tcactgtgat
    53521 attcaagaag ccaaagtaga aagtatccga gaatatgcat ttgaaagctt tggtaaacca
    53581 catacgaaga aggcgaatat ttataatttt gaagatttca agcaggtaca taatgataaa
    53641 tacaataatc agaatagagt tatagggagt gattgtggaa atccggcgta gtgaacttca
    53701 acaagagatg tttgtagtat tagatacgca aatatatatt gatctacctg atgatactaa
    53761 aagcaaaaat attgatgacc tggatattgt cttaaagatt tataagaata atgaggatga
    53821 ccatcttgtg cgtatctatt atgagatcaa agcaaaaaaa acttatccgg ataaaccagg
    53881 attgtgtata agggtgcaag cttcaggaga ttttagaatt gatgcttcaa tagatgtgaa
    53941 ttcaattcaa tttgggcagt tggtgaactt ctctgcaact gctatcacat ataataacat
    54001 tcgcgcttat ctacagaaca taacttccta ttatcctgtt gatacctata ttcttcccgc
    54061 aattgattta aacgatctat gcaagaaata ctatgtgaat caaaaagaag aaaatcaaga
    54121 atagataagc tgattcaggt tatttcaaac tttgtttctt ttttttgtta taacaattgg
    54181 gagaaaggta gttagaaact attttacgga tatacatagg atatttaatt gaaataatta
    54241 gtttggcacc aaggttaaac ttaactaatg ccatagaaat aattaataat atatagcaat
    54301 ccaatttcca agttctgatt gtacaaatat taggtataaa tttaaacaaa atccttcctt
    54361 gtctttggca aagaaagatt ttgccaactc atttttctaa cattgcatta tttgggttaa
    54421 aagtataagg atttctgttt tcacagggat gacaaaagaa ttagcaaaag ggaaaggata
    54481 taactcctta tgccaagaag cattagttac gcaatacgga ctcattgcta atgccatcag
    54541 taatgg
//
LOCUS       DAOPFL010000003        39568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_928_length_39568_cov_97.086478, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000003 DAOPFL010000000
VERSION     DAOPFL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 39568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39568
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_928_length_39568_cov_97.086478"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /gene="lon"
                     /locus_tag="PLG69_00685"
     CDS             complement(<1..2179)
                     /gene="lon"
                     /locus_tag="PLG69_00685"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPI42054.1"
                     /translation="MNYTTKIPRTLPVLHMSNVVMFPYLLMPLVVSDEESKLVIDYAL
                     ANDKLMAFFLDQEKDDTGITELANFGTAVTILRMLRNQDGSISMLLQGSTRIKLQKIV
                     QKNPFIMVDVEAIPEQFEEDTEIQAYRTVALELLEKIAQESNILNREMITGLSNIKQA
                     GRVADIIAGNIDLPISDRQKILETIDLKQRFRYLNNCLAELIKQMKVENHIRSNIQLE
                     MSEDQRRYYLREQMDAIRRELGETDEVSKEIMKWQVLIEQKNLPDYVKETALEELERL
                     ATMQPVSSEYSVVRNYLDWIVNLPWREYSKDRLDLKKIERILEKDHYGLKEAKERILE
                     FIAVKKLKGNLKGPILCFVGPPGTGKTSIGKSVARALSRKFIRMSLGGIHDEAEIRGH
                     RRTYIGAMPGKIIMEIKRQGTANPVFMLDEIDKVGRDFRGDPSSALLEVLDPEQNNSF
                     VDNYINLPFDLSEVLFITTANTLDTIPPALRDRMEIIEFTSYLENDKIEIAKHFLIPR
                     EKENNGLAKEKITFTKAALQEIIRYYVREAGVRNLQRRIGSIFRKIAKEVAMGTHQKW
                     IIKAEDIAKYLGPRKLTLEMANRKPEIGVATGLAWTGYGGEILFCETLRMPGKGNIIL
                     TGLLGEVMKESARIAVSYLKANHSVFIIPPKLFETSDIHIHFPSGAVPKDGPSAGLTL
                     TVALASLFTGQKVRHDIAMTGEITLEGKVLAIGGLKEKLLAAKR"
     gene            complement(2294..2794)
                     /locus_tag="PLG69_00690"
     CDS             complement(2294..2794)
                     /locus_tag="PLG69_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPI42055.1"
                     /translation="MIELETLAKIPLFYDVNSEALAYLQPRFKQLLVNKGTVIIKENT
                     EGDQIYILVKGIVQVTKDLVKGFDEDQLLTEKVLAKLSADSLPTFGENGVLGNGTRMA
                     NIIALSDCELYTLSKADFDNFAEYNYQAGYIVMKNIARKLSESLKDTDENLVKLATAL
                     YIAVQG"
     gene            complement(2806..3978)
                     /locus_tag="PLG69_00695"
     CDS             complement(2806..3978)
                     /locus_tag="PLG69_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPI42056.1"
                     /translation="MKLFLHPFRKISDRLSIRGEDQKLFIRFLWVCSFIAILLLISAL
                     LAWAFEQKGTQENTIKSFWDGIWWAIVSIATVGYGDKYPVTFQGRLVGIILIIVGYSS
                     LSFFTGLVASLFVEDRLKGAKGLKTIRTHNHIVICGWNNTAEFFLKALLEKQVSEMDI
                     CIVINATPDFFERLESRFPTLSLKFVRGDAIQEETLKRASVDTSAQVIILADEELDPS
                     VADDHSIIVANAVHYLTKNDKITVQLVNPENRSMLQRLGIRNIIIWDDIGGYILANSI
                     ADNNYLAVFCQLVQDKQNHLQTQKIPSDFIGKSFGDLSDYYYRECGYLLLGLLTKEPG
                     LELSSIFTEDISGIDQFIQYALTKSQKKIREEKSNIRWKPEKDVQLQKNDYAILMI"
     gene            complement(3982..4560)
                     /locus_tag="PLG69_00700"
     CDS             complement(3982..4560)
                     /locus_tag="PLG69_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42057.1"
                     /translation="MIFLKSLTFILWNIALGTLLVLLLNWLLFNRKARYLFGKKIPLT
                     PGFFVAKRDWLFDKARSILHDYLDQAAHPYLKDGYLYGWIKKVRQYLWEKTSFIDEWR
                     FLPAKLKLLVRNKIVDAFTAIAESILRKTVPRLVEQLQIEHRIDEFDIQFSVDFFYGY
                     FKRYVYKPMLLICAGLNLLFGILNMVWFLIIV"
     gene            complement(4561..5322)
                     /locus_tag="PLG69_00705"
     CDS             complement(4561..5322)
                     /locus_tag="PLG69_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPI42058.1"
                     /translation="MKYKRFCAVLPFVFLTALLFSVVVPKQVGFVNDFANILSAETRN
                     KINDWAIELREKTDVDFVIVTLPDIGGEDIVDFGVKLYEQWGIGSKRDEGVLILLALK
                     ERKLRFEVGYGSEGYLTDAYISEVYQTMKSYLTKGNEKWDDAFIQGSLMLLSTIAKEK
                     GITLTGVSEYSQKKKESSGGVGLLAFLILFIILIIATRGRILEWLFWMSIFGGRGGGG
                     NSGSWSSGSGGFGGFGGFGGFGGGRSGGGGGGGGF"
     gene            complement(5312..6544)
                     /locus_tag="PLG69_00710"
     CDS             complement(5312..6544)
                     /locus_tag="PLG69_00710"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPI42059.1"
                     /translation="MTNPEQEVDKTIFASIEEAIEIIRKGDMLIVVDDENRENEGDLL
                     MAADKITPEKVNFMISQGKGLLCAPLDIEIANRLDIPLMTNKSSDRQGTKFAVSVDVI
                     EGTTTGISAIERAKTIKTLADPQANASMFMRPGHIFPLIAERGGVLRRAGHTEAAVDL
                     AKLAGLSPVGAICEIIREDGEMARLDDLLPFAKKHNLKIITIEALIHWRRKKERLVHP
                     VSKAQLPTKYGQFDIITYISDISDEYHIALVMGEIKRDEPILVRVHSECLTGDALFSL
                     RCDCGEQLARAFQMISEEGKGVILYMRQEGRGIGLLNKIKAYHLQDEGVDTVQANIKL
                     GFPPDLRDYGIGAQILKDLGIRKMRLMTNNPKKIVGLSGYGLEIVERVPIEIKPQENN
                     REYLKTKKNKMGHILNEI"
     gene            complement(6537..7571)
                     /locus_tag="PLG69_00715"
     CDS             complement(6537..7571)
                     /locus_tag="PLG69_00715"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPI42060.1"
                     /translation="MKDKLKVIYTLQEMQRLDKFLAELKIQELYSRTFIEHLIEEERV
                     LVNLTPVKKSYLLKEGDEVIINLPEPEPMNVLPQDIPLDVVYEDEHLAIINKKAGMIV
                     HPGFGNPEGTLVNAILYRFGENLSSGKEFNRPGIVHRLDRGTSGLIIIAKNDLTQAKL
                     SDMFARREIKKTYFAITTGIPEPEKGEIETYLARSNKNPRTICVTNEGRYALTHYKTI
                     KTWHYFALVEINPATGRMHQIRVHFAHIRYPILGDLLYNSRRYVQSLVPQNMKKKVTE
                     LLLNHLRRQALHSWKLEFLHPITGYPLSVSAPIPDDIQYTLNWLDNNFSIDTDTSDGN
                     LILNEEIYND"
     gene            complement(7561..8298)
                     /locus_tag="PLG69_00720"
     CDS             complement(7561..8298)
                     /locus_tag="PLG69_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42061.1"
                     /translation="MKRSFKARYHILIAILFGIIVLQTLALLFSVARAQDLTTLANDI
                     HSIVIIFVFIIFVYIVILYNYIPFRLHKAIREVEQLIEEISNGNYQIDIDSSIYDQDK
                     DFQELIYALDKMLGIIIRFDQAKADKIFEHHQRIQLLINLLPQSILITGANGDVVYCN
                     EPLRKRFPMISETVNLNELIWKNDYHNRIFSVILDSLRYGNNIYDIIVEDVVYLGKVK
                     INGSIVRNRKGLSTGAVYVLEFYDNER"
     gene            complement(8305..9009)
                     /locus_tag="PLG69_00725"
     CDS             complement(8305..9009)
                     /locus_tag="PLG69_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPI42062.1"
                     /translation="MARYAVIGLGRFGMTVAKILTENGMEVIAIDKDQDLVNEISSIV
                     TSAVCMDSTDEAALRSQNLNEADAVIIGIGSNIEECILTAAILKKIGVGIIYAKIESE
                     LHGRILKMLGVQHTLLPEEIVGNQLAKTLISRNVLDYINLSSGHIVVEMVAPKEFVGK
                     PLQELALPTKRGVNVIAIKYNYLSVTEDGQNVIEKRLNDMPGANDIINEGDVLILLGP
                     KGNIDKLIYETAVKKD"
     gene            complement(9039..10733)
                     /locus_tag="PLG69_00730"
     CDS             complement(9039..10733)
                     /locus_tag="PLG69_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPI42063.1"
                     /translation="MPAEKKSGTFKHIELIAYILAFWSFFVLFLEPIFEYYTSSYSTI
                     EFITALANLVLLVLTILSRVLSREVKENKTVVYFDLVMLILGSLLLTYQAKFVIFFLL
                     IRQTWFIVQFILFRAFEGKIYKLLMRNPPVSLMFSFAGVILIGTILLMLPASSTEGYI
                     TNFVDALFTATSATCVTGLIVVDTGTYYSLFGQIVILLLIQVGGLGIMTISTAFALML
                     GQRISLNVTNLMHNVVGKTPVIDVLKLLRSTVLVTLIIEITGALFLFTSFAKLMPAGQ
                     AIYYSLFHSISAFCNAGFSLWSNNLAGFVGSPLVIFSFSFLIILGGLGFAVLIDIYHY
                     FFSSRRVRKLALHSKIVLSTTAILIVTGFLGLYIGEYNNSMKGFSVAGRVLSSWFQSV
                     TARTAGFNTIKIGSYSSASVLLTVLLMFIGASPGSTGGGIKTTTFSTLMLSVLSMLTG
                     KRDLVVFKRKISLANAREATTLATLALAIILTIIFILMLIEPFPFEKILFEAFSAFGT
                     VGLSLGITSQLSYAGKLLITLLMYIGRIGPLTLVYAISIRKRQPHISYAEERISIG"
     gene            complement(10748..11380)
                     /locus_tag="PLG69_00735"
     CDS             complement(10748..11380)
                     /locus_tag="PLG69_00735"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPI42064.1"
                     /translation="MLDVIVFLGIQGSGKGTQAELLAERTGFQHINIGDLFREEVHKQ
                     TELGKKVQAIIAKGELVQDELVFEIVNASLTADCAGVVFDGFPRTLAQAEYLLKNYNV
                     RRVYYLELSEEEAIARIEARRLCSSCGETYNILSKKPKQEGICDKCGSPLVQRTDDSG
                     EAIRERVKAFYEQTFALKEYFAEKGLLKEISARKSIEEIQQEIINDLKIR"
     gene            complement(12458..14467)
                     /locus_tag="PLG69_00740"
     CDS             complement(12458..14467)
                     /locus_tag="PLG69_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI42065.1"
                     /translation="MNKIAKIIVLIACIAIIWTGLTADEVIIGSGTQTARIPLDFFYQ
                     NSLFECLYYQNELGFAQGTISRIAFYNNFVTLLNNKPTKIWLGTTNLTTLNAGWIPST
                     ELTLVFDGQVNYPSGIHIIDIELDTLFHYTCGTLVLMVQRPMDSGYFSSSDKFYCQTD
                     TMFRARNAYHDTQLLDPANPPASGFVLNGQYPKIRITYTAQSIASDLSCSQVISDKVV
                     TLGATEQVQVKINNYGYEDQDNYIVNLKKGDGELLATALGNPVSAGQTLIQTLMWVPT
                     TPGTYDIFAEVVLNGDQDLTNNQSPHINVTAFEAGFFSLTIGIGNLSGNLPLDFSYHT
                     SLFETLIYAPEIDTTGIITHLIFYNNFIENINDKPVIIWLGETDLVNLPLTWIPSSNL
                     AMVYYGTMSFPAGQNEIIFPLYPTYNYQGQNLVLMVQRPFDYNVYNSNNVFRMQSSAM
                     QRTKYIYSDQQEHDPGAPPLYAFDSYQFPQTGFIMRPPNTGFLRGTVRNGTDLVIGAT
                     VMADGSQTTYTSSAGTYSLLLSSGLHNITASYPGLNTVTRQVNITAGIVTTEDFNLEG
                     NAVEDPQTPHISTSLKGIYPNPFNPETTISFSIYRDNTPIVIELYDLKGRKVKTLINS
                     VYNRGEQQFHFSAKDEKNQDLASGVYFIRLAAPDYSKTVKALLLK"
     gene            complement(15407..15784)
                     /locus_tag="PLG69_00745"
     CDS             complement(15407..15784)
                     /locus_tag="PLG69_00745"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPI42066.1"
                     /translation="MIIGIGCDIIEVERIQNAIEKNSRFCEKLYTASEIEYCSGKANS
                     YQSFAVRFAAKEAVMKALGTGWNGEVNWIDIEVVTSEKGSPTINLTGGAKDLADKLKV
                     NNICLSLAHEKGYALAFVILEGE"
     gene            complement(15784..16248)
                     /locus_tag="PLG69_00750"
     CDS             complement(15784..16248)
                     /locus_tag="PLG69_00750"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPI42067.1"
                     /translation="MSERVFYNSPDGFFHLLLEIEEETIMSINFCPAQVSSQPSNDLE
                     REIFRQFDLYFTGRSKEFDLPFFATGTVFQLMVWEEVMRIPYGNTLTYKEIAERIANP
                     NAGRAVGKALNKNPVPILIPCHRVIGSKGKLTGYGAGIAIKKKLLELERSNL"
     gene            complement(16595..16948)
                     /gene="rplS"
                     /locus_tag="PLG69_00755"
     CDS             complement(16595..16948)
                     /gene="rplS"
                     /locus_tag="PLG69_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPI42068.1"
                     /translation="MDILQQVSRDQIRTDLPDYRVGDTVKVHYKIKEGNKERIQVFQG
                     IVIQKRGAGISKTFTVRKVSSGVGVERIFPQNSPNIDKLEIIRHGQVRRAKLFYLRSA
                     QGKAGRIKEKRRFTV"
     gene            complement(17171..17842)
                     /gene="trmD"
                     /locus_tag="PLG69_00760"
     CDS             complement(17171..17842)
                     /gene="trmD"
                     /locus_tag="PLG69_00760"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPI42069.1"
                     /translation="MIFEVLSLFPDAFSGFLNSSIIARAREKKLLEVNLTDYRQFSLN
                     KHHKVDDYPYGGFAGMVIQAQPIYSALIELLEIGNAPVVYFTPQGRPLTQKILESYSS
                     YKRIILLCGHYKEIDQRVRDLCVSDEISLGDYVLTGGEIPALAFIDGVSRLLPGVLSD
                     RESANSDSFSNEGLGFPCYTRPEIFMSLGVPEVLLSGNHKEIKKWALEQGKRLTVTRR
                     PDLKK"
     gene            complement(17839..18369)
                     /locus_tag="PLG69_00765"
     CDS             complement(17839..18369)
                     /locus_tag="PLG69_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42070.1"
                     /translation="MKHPPLIGIGKLGRQNADGFHPVMIKPEYRSVFSELEDLYLIFN
                     SDRVFYVTISERMRKDNKTWIKLKEDGVSEEQFLHKDVVIAIDDTAIETEETSLNYLI
                     GYQVIFAGEELGIVEDFFFNGAQDVLQIVDSKGTEYLVPFVDYYIDTVIDNLRCVILQ
                     NAVDLVALYKVEAKKK"
     gene            complement(18370..18600)
                     /locus_tag="PLG69_00770"
     CDS             complement(18370..18600)
                     /locus_tag="PLG69_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPI42071.1"
                     /translation="MKDLIEFIVKALVDDPSEVNITEITGDKITLYELRVSKADIGKV
                     IGKRGRTASAIRTIINAVSTKQGKRAELEIIE"
     gene            complement(18627..18920)
                     /gene="rpsP"
                     /locus_tag="PLG69_00775"
     CDS             complement(18627..18920)
                     /gene="rpsP"
                     /locus_tag="PLG69_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPI42072.1"
                     /translation="MVKLRLRRMGAINQPFYRIVAVDSRKKRDGKYIECIGWYDPKPD
                     PSKIKIDTERALYWLGVGAQPSDTVRSLLRKAGVLQIWHNNKQMKNQKTAEAE"
     gene            complement(19037..20359)
                     /locus_tag="PLG69_00780"
     CDS             complement(19037..20359)
                     /locus_tag="PLG69_00780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPI42073.1"
                     /translation="MKIAVIGSGYVGLTTSACFAEMGNTVISVDKDENKIEMLQAGKV
                     PIYEPGLEDMILRNMTAKRLNFTLDTKQAVQDSQIIFIAVGTPPGEDGSADLQYVIAA
                     AEEIASYLNEPKIIVNKSTVPVGTADLVNEKIQEVLNARGVDINYAVVSNPEFLKEGS
                     AIDDFMSPDRVVIGTDNPKAGEIMRTLYEPFCRTNDRILIMGIRSAEMSKYAANSFLA
                     TKISFINEISRLCDAYDADVAEVRNGMCSDSRIGYKFIFPGVGYGGSCFPKDIKALIN
                     MSRKVGYEPRILKAVEEVNSEQKKVLVEKVKAHFGKNLKGKTFAVWGLAFKPQTDDMR
                     EAPSVVIINELIAMGATVKAYDPVAMQEAKKLFGNNNSVTLCADEYETLKDAVAMLLI
                     TEWHQFRYPDFERMSKIMRQKVIFDGRNQYNPKAVKEMGFTYYGIGRR"
     gene            complement(20356..21288)
                     /locus_tag="PLG69_00785"
     CDS             complement(20356..21288)
                     /locus_tag="PLG69_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPI42074.1"
                     /translation="MKILVTGGAGFIGSNVADAYLQAGHEVVIVDNLVTGNRRNINPK
                     AIFYEMDICDESLSEVFAKEKPDMVNHHSAQISVPLSIENPLLDVINNVYSWVNVLQN
                     CVRTGVKKVIYISSGGAIYGEAEEYPTSEKYNPKPLSIYAINKSVGEDYLYFYRHQYG
                     LNYTVLRYANVYGPRQISQGEAGVVSLFTEKLLKGEIPTVYRYDNEPDGMIRDYVYVG
                     DVVQANLLALERGEGEVFNIGTCIPTTTKDLYYAIAKQLGINREPYYGPARKGDLHRS
                     LLSCEKAKKVLGWKPETGLSEGISQVIKYFKELQ"
     gene            complement(21285..21860)
                     /locus_tag="PLG69_00790"
     CDS             complement(21285..21860)
                     /locus_tag="PLG69_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HPI42075.1"
                     /translation="MATHKPVYVDELYGELALFTDDRKWVVVHTKPRCEKKLADYAKQ
                     HQITYYLPQYTSKRVYQRRKVDVNKVLFPSYVFVGIDFKGKQELLISGYTVNFLKVNA
                     QKELIDELQAIYYGRQKNVQMEPGLWLEKGLEVEIINGPLKGIRGVVEDQSKLTEVRL
                     QVNILKQAVLVKVRTQDIKIIGEFEIVEINE"
     gene            complement(21863..23263)
                     /gene="ahcY"
                     /locus_tag="PLG69_00795"
     CDS             complement(21863..23263)
                     /gene="ahcY"
                     /locus_tag="PLG69_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPI42076.1"
                     /translation="MDYKIADLSLADWGRKEIDLAEAEMPGLVALKERYRHRKPLAGA
                     KITGSLHMTIQTAVLIETLVELGAQVRWASCNIFSTQDNAAAAIAQRGIPVFAWKGET
                     LNDYWWCTLQALSWEEGPDLIVDDGGDATLMVHEGYRLEEQYKQTGKLPEVDAENAEM
                     RIVQELLINQLTIDPYKWHRAVKCLRGVSEETTTGVHRLYQRRNEGTLLFPAINVNDS
                     VTKSKFDNLYGCRESLADGIKRGTDIMVAGKIVMICGYGDVGKGCAQSMRGLGARVVI
                     SEIDPICALQAAMEGYEVNTVENMVEIADIFITATGNCDVIRVDHILKMKNNAILCNI
                     GHFDNEIQVNKLYEMEGVEKVNIKPQVDLIKLPNDKAIILLSEGRLVNLGNATGHPSF
                     VMSCSFSNQVLAQIELWQNEHEINVYTLPKKLDEEVARLHLPKLGAQLTQLSKKQAEY
                     INVPIEGPYKQDYYRY"
     gene            complement(23285..25516)
                     /gene="pnp"
                     /locus_tag="PLG69_00800"
     CDS             complement(23285..25516)
                     /gene="pnp"
                     /locus_tag="PLG69_00800"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPI42077.1"
                     /translation="MHNFNITRRETDFCGRKLIVETGKMAKQANGSVFIQYGETAVLV
                     TATMSKEPAEDQDFFPLTVDYIEKMYAAGKIPGGFFKRESKPSTDATLMARVIDRSIR
                     PLFPEGFRNQVHIVVTVLSFDGVTDPSTMGVFGASLALSISDIPFNGPIASVTVGYID
                     NQFVVNPHFNEIETQSKLNLTVAGSESSIVMIESAASELSEEMMLDAVFTGHKEIQKL
                     CALQQDFISACGKEKVEVVLDSVPEEIMNKVENAFGAKIAEAAVIFGKQERQDAFDAA
                     EAEMVEMFSQQEDTDLSPWEETERYYKMAFDELIRRYVRDSILRKQHRVDGRGTDDIR
                     EITCEIDVLPRVHGSALFTRGETQSLGTLTLGGGTDEQVIDGLETEYKKSFYLHYNFP
                     PYSVGEVGRIGPTGRRELGHGNLAERALKAVLPDKDSFPYTIRIVADTLESNGSSSMA
                     SICSGCLALMAGGVPIKAPVAGIAMGLIMEGEDYVVLTDIMGLEDHLGDMDFKCAGTR
                     EGITAMQMDIKISGITREIMQIALNKAKSARFKILDIMAECIAEPRSDLAPWAPRIES
                     FKVPIDKIGEIIGPGGKMIKSIIEECQVQIDIQDDGTVRILSPDKESINKAKSIIKGI
                     AVDPVAGDMFEGQVTRIEPYGIFVKILGGTKEGMVHISEMYTGRINHPLDMVKLGDKV
                     QVRTLGMDKGKLSLSMKGVAGNPEPHPDAHREEQQNYHQQNRDDRNRGRNSGRNDYHR
                     HHR"
     gene            complement(25794..26063)
                     /gene="rpsO"
                     /locus_tag="PLG69_00805"
     CDS             complement(25794..26063)
                     /gene="rpsO"
                     /locus_tag="PLG69_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPI42078.1"
                     /translation="MPLKPEAKQAIMAEFKLHESDTGSPEVQVALLTQRIKDITEHLK
                     EHPKDFSTRRGLLKLIGQRRRLLDYLKKKDITRYRNLIKTLGIRK"
     gene            complement(26311..26664)
                     /gene="ssrA"
                     /locus_tag="PLG69_00810"
     tmRNA           complement(26311..26664)
                     /gene="ssrA"
                     /locus_tag="PLG69_00810"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            27346..28023
                     /locus_tag="PLG69_00815"
     CDS             27346..28023
                     /locus_tag="PLG69_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42079.1"
                     /translation="MKISAIFYAIVILAFFMPFFMVSCDQTEVASLSGVKLVIGGEVK
                     LSIAEEIEKIEDVGKKYQNPVVNIQPFALIALFIAVLALVLVLVLPIQYYLLPAYISI
                     IGVLSLQLLNVGILQIITAYQPLFDPSIDLNSILIIKAQPAFWLANLAFILGGIFTLI
                     AGVKSSSISAPAQEPEEVQEEYITSEIQEWVEEYPSEETFSDSDEENLPAEEENSVLI
                     EEKNTPE"
     gene            28035..29093
                     /locus_tag="PLG69_00820"
     CDS             28035..29093
                     /locus_tag="PLG69_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPI42080.1"
                     /translation="MDSAKELRWIRILLLIIALAVVASILKTLKSIFIPLIFSIFLLF
                     LFAPLINYLKKHKVPMVLILLITLVIIAVFLGLVILLIYAASNSLINGLPRYEDKFNE
                     LIAKGTEYLQNLASNWNINTENISIANIAQIISSGFISIPQFISNTVNTLVSIIQNIF
                     LIIFFLIFLLLEIEKLPLRLRKATSKLSKEQTLDILQNIEKQIQNYLTIRTLVNLSAA
                     LLCMLWMLIFGVDFILVCGILLFVLDFIPDVGSVISSAIPILIYLLQSGFSFLWLIFA
                     LLIVATQMLIGNIIEPKLQGVQLNLTPIMVLVSLIFWGWLWGIVGMLICVPLTSAINI
                     ILKQVAPNNFISTLISSE"
     gene            complement(29392..30129)
                     /locus_tag="PLG69_00825"
     CDS             complement(29392..30129)
                     /locus_tag="PLG69_00825"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPI42081.1"
                     /translation="MPIQEEFEKSGAWIFKWRSYLPLLMFILGIGAILDLKNSQFIKY
                     DTIIEVICFSISGCGIAIRVLTIGHTPSGTSGRNTKSQVADKLNTTGMYSLLRHPLYF
                     GNFFIILGVVLFTRHIWVIIIYPLIFTLYYERVIFAEEAFLRKKYGEEFVSWASKTPV
                     YFPKFSHYKKPEFPFSWKKVIRNEYQTFFALICSLFALEIISDLIIRQRFVFDPAWKI
                     IFITSCLFYLIIRFINKKTKLLHTSGR"
     gene            complement(30364..31578)
                     /locus_tag="PLG69_00830"
     CDS             complement(30364..31578)
                     /locus_tag="PLG69_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPI42082.1"
                     /translation="MSEINNKRERKNIFRKLIHFFALNSSMLGMLIMVILVGMGEKMA
                     ERFLPLYLIAMGGSSIVIGLLNGMDNLLSALYSFPGGWLADRIGYKKALKIFNLMAML
                     GYLIVVIFPSWIAVLVGAVFFISWTAISLPATMSLINSVVPANRRSMGISLNSIVKRI
                     PMSLGPILGGALILHYGRIQGVRYAFIVAFILAGVALFIQDKFIQEQPPEAKTERKKG
                     RGLFSANLRNLLVSDILVRFCEQIPYAFVVVWVVENNHINELQFGILTTIEMVTAMLI
                     YIPVAYLADRSTRKPYVAITFVNFTIFPLVLLFSHSFPAMVIAFIIRGLKEFGEPTRK
                     ALIMDLAPENAKAYTFGVYYLIRDVIVAFAAFGGAFLWQKSPQLNFLTASAFGLLGTL
                     YFIFRVKPQKQF"
     gene            31913..33103
                     /locus_tag="PLG69_00835"
     CDS             31913..33103
                     /locus_tag="PLG69_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HPI42083.1"
                     /translation="MIEVLIEKCVGCGACLRACAYDAIKIEEKLAIIDSDKCVLCGAC
                     VSACPFDAILLRKAKTEAIDKSEYNGIWVFAEQREGVIAPVVFELLGKGRELADELGS
                     ELSAVLLGHNIEGLSSELIAFGADQVIEIDDPALKHFWDEGYAYALAELALKYKPSII
                     LAGATVIGRSFIPRVAIRLHTGLTADCTGLSIDPETGDLLQTRPAFGGNIMATIKTAN
                     HRPQMATVRHKVMNPIPRDDTRSGIVIQEQINFDLDEERTTWLGFEKEKTGLINITEA
                     NIIVSGGRGLKEAKNFAIIEELAEALGGAVGASRAAVDAEWIAYSHQVGQTGKTVKPL
                     IYIAVGISGAIQHLAGMSSSDYIIAINKDPDAPIFKVADLGIVGDLFDIVPKLTKRIK
                     EQRV"
     gene            33100..34311
                     /locus_tag="PLG69_00840"
     CDS             33100..34311
                     /locus_tag="PLG69_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPI42084.1"
                     /translation="MIIKPAISVISPDRMEQGIDLYLWGSPFEEIDLRKDCSNAIAYI
                     PLINSHDHLISNWVPRAGNNRPYKNSHIWVEDMKESFAFQERDIFWHNNGSFRLEEPN
                     SLILAKLGAYKSLFCGCSVVQDHSSNQVDAYYEAMPIVVPKNFRQCHSLTLGNWWGGE
                     TPEKEMELTKGKMPFIVHLGEGKDEITKGEFSQLKKMGLLRANTLIIHGIALTAEEIS
                     EVANVGASICWCPSSNFFLIGETLKIDEVLKHKANVCIGTDSTMSGGVNLIAEFLKIR
                     EHFPKIPTKEIYRMATVNAATALYLPQYFAFIIPNKCRNLLLVDKVERDPYENLFALE
                     ASTIQLLLVEGIPRFGDSEWMEILNLDENMYSTFRTGNKEKFVIGDPMELNDQIDEVL
                     GYHKDFPYLPF"
     gene            34441..35070
                     /locus_tag="PLG69_00845"
     CDS             34441..35070
                     /locus_tag="PLG69_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPI42085.1"
                     /translation="MEKHLNVTEIYYSLQGESAYSGYPCIFIRLSECNLRCVYCDTQY
                     AFGKGKSMAISSIMEEVKKYPCSLTEITGGEPLLQEDVDALFEELYKFSYKILLETNG
                     AISLEKVPDYVIKIVDVKTPGSGMEKSFLPENLQYLNPQDELKFVITDKVDYQFAVDF
                     LKKYKPQVRCVHFSPVTELLNPKELACWMLEDGLEARLTLQLHKIIGMA"
     gene            35304..36293
                     /locus_tag="PLG69_00850"
     CDS             35304..36293
                     /locus_tag="PLG69_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42086.1"
                     /translation="MKSVYGLLLIGVILVFFSPALLSDTVKLTNGNVIYGHITREDID
                     KVTIVDQNGIENIIPRNLIIFIDKQLETTREVQKVIITLKSGIEVKGTLILDDANSVT
                     IKDESGIEKKIAKSNIRDIVIDLDKPDERPITIKSYEEPETEIETETEAEIVTTPAEK
                     TNNFDFGLKFGSQKPNSNAISDKYSNGIVYGGELIFWLKNKYGIGLNVEKFSEKSEYW
                     FYNEGNYEYNISIIPITLTVYYKHYNNKIVPYIGLGIGQYNLTEKIKYPWYYYYKELE
                     VSDEEYGFHCLFGLQYKILFVEVKYSTMFKSTWGAEEEYDDIGGLSIAGGLRF"
     gene            complement(36484..36696)
                     /gene="thiS"
                     /locus_tag="PLG69_00855"
     CDS             complement(36484..36696)
                     /gene="thiS"
                     /locus_tag="PLG69_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPI42087.1"
                     /translation="MTDKPVFFVNGNKLPWKEGLVVADALKMMNYTFKMLVIKMNGEL
                     IKRENWQNIPIPENADIKVIHLISGG"
     gene            complement(36700..37317)
                     /gene="thiF"
                     /locus_tag="PLG69_00860"
     CDS             complement(36700..37317)
                     /gene="thiF"
                     /locus_tag="PLG69_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPI42088.1"
                     /translation="MISREEYFSKRDPSAFPLWQKATVGIAGAGGLGSNIAFSLARAG
                     IGTLIIADYDIVTLENLNRQQYTIAQIGKLKVEALAENIQSFNPFINLVLHPVKITPA
                     NFNAIFGTADLLLEALDEAEQKEMLISCWTSIYPNKHLIVASGLAGYGKNESIHTEHY
                     GFLHLVGDMKSELNAEISPVATRVAVVANMQANLALELLAETKGM"
     gene            complement(37314..38063)
                     /locus_tag="PLG69_00865"
     CDS             complement(37314..38063)
                     /locus_tag="PLG69_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HPI42089.1"
                     /translation="MKALGIIGYHQTGKTTLATCLIRELTKKGYNVCSIKDIHSETYH
                     SDTEGKNTFLHTQAGSKAVFAKGLYDSALLFPRPLNLKEMVSYLQADYLIIEGLQNAP
                     VSKIVCAENTDQLDELIDDTCIGISGVISSKMKSFKGLPVFSLPDDLAVLLSTIEAKC
                     FDILPLAEPECCSACGKDCYTMAEDIIQGRAKRSDCVLAEQAELRLWVGDREIVIVPF
                     VQKLLQDIVIAFVNNLKDIDPKGNIRLEIKR"
     gene            complement(38066..38542)
                     /gene="ispF"
                     /locus_tag="PLG69_00870"
     CDS             complement(38066..38542)
                     /gene="ispF"
                     /locus_tag="PLG69_00870"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPI42090.1"
                     /translation="MFRCGFGYDVHCLVSDRELILGGVKIPYSKGLSGHSDADVLIHS
                     IIDALLGALALGDIGTFYPDNDLAYKDIDSRILLRKTYTLITEKGWKLNNLDAVICCQ
                     EPKLQSFINAMRANLALDLHCAIEQISLKATTEEGLGISGKGEGISAFCQLLLVTD"
     gene            complement(38547..>39568)
                     /locus_tag="PLG69_00875"
     CDS             complement(38547..>39568)
                     /locus_tag="PLG69_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HPI42091.1"
                     /translation="VAIPILCGPEIAVPATKSFMNQITLFYYLAIKTAQMKLNEIDNN
                     LTHEQRLWRQQELDDYFVSLAKIPSLLKETLDNVSGEIEFMAGKIYMEPSIHILATKI
                     SGVAMEGALKIRETVLTHAEGREASEFKHGPNTILGKNTVFGVKHLRSLLRYFSENID
                     EIESLCEQEGIPHSETKEVYKALADYIFTHNQPFNLSPQGTNIFNRFVQDKDFFEHLY
                     RNYPLIYVTGPDERDVNLTISQINTHKIRGANTFVIAEENEQLLKNASTRPSENSYYA
                     WSYIMLPKTGSTLLTCFSASIVLQLLALKMSVRKMKKLDKLGVLDHGVHPDVPKNVSK
                     SITVD"
BASE COUNT        12204 a         8076 c         6921 g        12367 t
ORIGIN      
        1 ctctttttgc cgctaaaagt ttttctttca agccaccgat ggctaaaacc tttccctcta
       61 aagtgatttc acctgtcatt gctatatcgt ggcgcacttt ttgtcccgta aaaagtgaag
      121 ctaaagctac cgtaagagtt aaaccagctg aaggtccatc cttaggtaca gctccagaag
      181 gaaaatgaat atggatatca cttgtttcaa aaagtttagg gggaataatg aaaacactgt
      241 gattagcttt caaatagcta actgcaatgc gtgccgattc tttcatcact tcgcccaaaa
      301 gtccagttaa aataatattt ccttttcccg gcatacgcaa ggtctcacag aaaaggattt
      361 cacccccata acctgtccag gcgaggcctg tagccactcc tatttcaggt ttgcgatttg
      421 ccatttccaa agttaattta cgcggaccta agtatttggc aatatcctcc gctttaatta
      481 tccatttctg atgtgttccc atagctactt ctttagctat tttgcggaaa attgagccga
      541 ttcttctttg tagatttcgc actcccgctt ctcggacata ataacgaata atttcttgca
      601 gagctgcttt ggtaaaagtt attttttctt ttgccaaacc gttattttcc ttttcccgag
      661 gaatcagaaa gtgcttggca atttcaattt tatcgttttc cagatagctg gtaaattcaa
      721 ttatctccat tctgtctctt aaggcgggag gaattgtatc taaggtattg gcagtggtaa
      781 taaacagcac ttccgaaaga tcaaaaggca gattgatata gttatccaca aagctgttat
      841 tttgttccgg atcaagaact tccaataaag cggaagaagg atcacctcta aaatccctgc
      901 caaccttatc aatttcatcc agcataaaaa caggattggc agttccctga cgcttgattt
      961 ccattattat tttacctggc atagcgccaa tataggttct gcgatgaccg cgaatttctg
     1021 cttcatcatg aattccgcct aaggacatcc gaataaactt gcgggaaaga gctctggcaa
     1081 ctgatttgcc aatagaggtc tttcctgttc ctggaggacc tacaaaacaa agaataggtc
     1141 cttttaagtt acctttcaat ttctttacgg caatgaattc caggatacgt tctttagctt
     1201 ctttcaagcc gtaatggtct ttttccagaa tgcgttcaat ctttttcaaa tccaaacggt
     1261 ctttgctata ctctctccaa ggcagattaa caatccaatc aagataattt ctaactacgc
     1321 tgtattcgct ggaaacaggt tgcatagtgg caagacgctc taattcttcc aatgcagttt
     1381 ctttcacata gtccggaagg tttttctgtt caataagtac ctgccatttc ataatttctt
     1441 tgctaacttc atccgtttca cccaattctc tgcgaatggc atccatttgt tctcgcaaat
     1501 aataacgacg ctggtcttca ctcatttcca gctgaatatt actgcggata tgattttcca
     1561 ccttcatttg tttaatcagc tccgccaaac aattgttcaa ataacggaaa cgctgtttga
     1621 ggtcaattgt ttccaatatt ttttggcggt cagaaatagg caggtctata tttccggcaa
     1681 taatatcagc cactcttcct gcttgtttta tattactaag cccggtaatc atttcccggt
     1741 tgagaatgtt gctttcctga gctatttttt ccagcaattc taaagctacg gttctgtatg
     1801 cctgaatttc ggtatcttct tcaaattgtt ccggtattgc ttctacatct accattataa
     1861 aaggattttt ttgcacaatt ttttgaagtt taatgcgggt tgaaccctgc aggagcatac
     1921 tgattgaacc atcctgatta cgcagcatac gtaaaatagt aactgctgtg ccaaaattag
     1981 ccaattcagt tattcctgta tcgtcttttt cctggtcaag gaaaaaagcc atcaatttat
     2041 cgttagccag tgcataatca atcaccagct ttgattcttc atccgaaact accaatggca
     2101 taagcaggta gggaaacatc actacattgc tcatatgcag aaccggtaaa gttcggggta
     2161 ttttagtcgt gtaattcact tcatctccta atgatttttt acttttagtg tttgcgaaaa
     2221 agatacaaat tcacattcat tcttttcaac tatataataa tcaattcaca gcaataagca
     2281 aattttagaa atatcacccc tgaacagcga tgtaaagagc agttgccagc tttactaaat
     2341 tttcatcggt gtctttcaaa ctttccgata atttccgggc tatgtttttc atcactatat
     2401 aaccggcttg gtaattatat tcggcaaaat tatcaaaatc cgccttagaa agtgtgtaaa
     2461 gttcacaatc gctgagagca ataatatttg ccattctggt tccattacct aaaactccgt
     2521 tctctccaaa agtaggaagg gaatcggcac taagctttgc cagaaccttt tccgttaaga
     2581 gctgatcttc gtcaaagccc tttacaaggt ctttagtaac ctgcacaatt cctttcacca
     2641 ggatataaat ctgatcgcct tcggtatttt ctttgataat taccgtgcct ttattaacca
     2701 gtaactgctt aaaacggggt tgcaaataag ctaaagcttc ggaattgaca tcataaaata
     2761 aaggaatttt tgccagtgtt tccagttcta tcataataag tccttttaaa tcatcaggat
     2821 ggcataatca tttttttgca gttgaacatc cttctcgggt ttccagcgaa tgttgctttt
     2881 ttcttcccga attttctttt gggacttggt tagcgcatat tgaataaatt gatcaattcc
     2941 actaatatct tccgtaaaaa ttgaagaaag ttccagtccc ggttctttgg ttaataatcc
     3001 taaaagtaaa taaccgcatt cccgataata ataatcggat aagtccccaa aactttttcc
     3061 gataaaatcg gaaggaattt tctgcgtctg cagatgattt tgtttatcct gaaccagctg
     3121 gcaaaacaca gccaaataat tattatcagc tatgctattc gccaaaatgt aacccccaat
     3181 atcatcccag ataataatat tccgaattcc caaacgctga agcatagaac gattttctgg
     3241 attcaccagc tgtacggtta tcttatcatt tttggttaaa taatgaactg catttgccac
     3301 aatgatactg tggtcatcag caacagaagg gtctaactct tcatcagcca aaataataac
     3361 ctgagcagat gtgtccaccg aagctcgttt taaggtctct tcctgaattg cgtcacccct
     3421 aacgaatttc agggataatg taggaaagcg tgattccaac cgctcaaaaa aatccggcgt
     3481 ggcattgata acaatacaaa tatccatttc ggagacctgt ttttccaaaa gtgctttcag
     3541 aaaaaactcg gcagtattat tccagccaca aatcacgata tgattatgtg ttcttatcgt
     3601 tttcaaaccc ttagctcctt tcagccggtc ttctacaaaa agagaagcaa ccagaccggt
     3661 aaaaaatgat aatgaagaat aacctactat aatcaatata atgcctacca accgaccttg
     3721 aaaagtgaca ggatatttat ctccataacc tactgtggct atagaaacaa ttgcccacca
     3781 gattccatcc cagaatgatt ttatggtatt ttcctgagtt cctttctgct caaaagccca
     3841 agccagcaaa gccgaaatta aaagcagaat agctataaag gaacaaaccc ataaaaaacg
     3901 gataaataat ttttgatcct caccgcgaat agacaagcgg tctgaaattt tccgaaaggg
     3961 atgcaggaac agcttcattt tttatacgat aatcaggaac caaaccatat tcagaatgcc
     4021 gaataacaga ttgagcccgg cacaaataag taacatcggt ttataaacat aacgcttgaa
     4081 atagccatag aaaaaatcca cactgaactg aatgtcaaat tcatcaatcc gatgttcaat
     4141 ttgcagctgt tccaccaatc tgggaactgt ctttcttaaa atgctttctg ctattgcggt
     4201 aaaggcatcc acaattttgt ttctaaccaa cagctttaac tttgcaggta aaaaacgcca
     4261 ttcatctata aagcttgttt tttcccacaa atactgacgc acctttttta tccaaccgta
     4321 aagatagccg tcttttaggt aaggatgagc cgcctgatca agataatcgt gtaaaattga
     4381 acgcgctttg tcaaacagcc aatccctttt ggcaacaaag aaacctggag ttaacggaat
     4441 ctttttccca aatagatacc tggctttgcg attgaacaac agccaattta aaagaagcac
     4501 cagaagagtt cctagggcta tgttccataa gataaaagtg agtgacttta agaaaatcat
     4561 ttaaaaacct cctccacctc ccccaccacc tgaacgtcca cctccaaaac ctccaaaacc
     4621 tccaaaaccg ccaaaaccgc cacttccgct actccaagaa ccgctatttc ctccacctcc
     4681 acgtcctcca aaaatactca tccaaaaaag ccattccaaa attctgccac gagtagctat
     4741 aattaaaata ataaaaagaa tgagaaaagc aagcaaacct acaccgcctg aactttcttt
     4801 cttcttttgt gaatattcag aaacgccggt taaagtaatt cctttttctt tggcaatagt
     4861 ggaaagcaac attagcgagc cctgaataaa ggcatcatcc catttttcgt tacccttggt
     4921 gaggtagctt ttcattgttt gataaacctc actgatatag gcatcggtta aatatccttc
     4981 cgagccgtag ccaacttcaa aacgcaattt tctttcttta agagctaaaa gaattaaaac
     5041 accttcatcc cgtttacttc ctattcccca ttgttcataa agtttaaccc cgaaatccac
     5101 aatatcttct cctccgatat caggaagagt aacaattaca aaatccacat ctgtcttttc
     5161 tcgcagttca attgcccagt cattaatttt attgcgggtt tcagcactta aaatattggc
     5221 aaagtcattt acgaaaccca cctgtttagg aacaaccaca ctaaacagta atgctgttag
     5281 gaaaacaaac ggtaaaacag cacagaaccg tttatatttc attcagaata tgccccattt
     5341 tgttcttttt agtttttaaa tactcgcggt tgttttcctg gggtttaatt tcaattggca
     5401 cccgttctac aatttccagt ccgtaaccgc ttaaacctac tattttttta ggattattag
     5461 tcatcagccg catttttctt attcctaaat ccttcagaat ttgtgcaccg attccatagt
     5521 cccttaaatc gggaggaaaa ccaagtttga tatttgcttg aactgtatct acaccttcat
     5581 cctgaagatg gtatgcctta attttattca gtaaaccaat tcctcttcct tcctgacgca
     5641 tatacaaaat cacacccttt ccttcttccg aaatcatttg aaaagcacga gccaattgtt
     5701 ctccacaatc acagcgcaaa ctgaataaag catcgccggt taaacattcg gaatggactc
     5761 ttaccaaaat aggttcatcg cgctttattt cacccatcac taaggcaata tggtattcat
     5821 ccgaaatatc actgatatag gttataatat caaattgacc atactttgtg ggcaattgtg
     5881 ccttggaaac aggatggaca aggcgttctt tttttctccg ccagtgaatt agcgcttcaa
     5941 tagtaataat tttcaggtta tgctttttgg caaaaggcag caaatcatcc aaacgagcca
     6001 tttcgccatc ttcgcgaata atttcacaaa tagcgccaac agggctcaaa ccagcaagtt
     6061 tagccaaatc aacagctgct tcagtatgac ctgctcttct taaaacacca cctctttctg
     6121 caattaaagg aaagatatga cccggacgca taaacataga ggcattggct tgcgggtctg
     6181 ccaatgtctt aattgtttta gctctttcaa ttgcagaaat tcctgttgtc gtgccttcaa
     6241 taacatccac ggaaacagca aacttcgtgc cttgacgatc actactttta ttagtcatca
     6301 aaggaatatc caaacggttg gctatttcta tatccaaagg agcgcagagc agacctttgc
     6361 cttggctgat cataaaattt accttttccg gagtaatctt atcagcagcc attaaaaggt
     6421 caccttcatt ttccctattt tcgtcatcca caacgattag catatcacct ttgcggataa
     6481 tttctattgc ttcttcaata gaggcgaaaa tagttttatc cacttcttgt tctggattag
     6541 tcattatata tttcctcatt caaaatcaga ttgccatcac tggtatctgt gtcaatacta
     6601 aaattattat ccaaccagtt caaagtatat tgtatatcat caggtatggg agctgaaaca
     6661 gatagaggat aaccggttat gggatgtaaa aattccagtt tccaggaatg taatgcttgt
     6721 ctgcgtaaat ggttcaacag cagttcggta accttttttt tcatattttg cggcaccagg
     6781 gattgcacat agcgtctgct attatataat aagtccccta aaataggata acggatatgg
     6841 gcaaaatgca ctctaatctg atgcattctt cctgttgccg gatttatttc taccagggca
     6901 aaatagtgcc aggtttttat tgttttataa tgagtgagtg catagcgtcc ttcattggta
     6961 acgcagatag ttctgggatt tttgttactg cgtgcaagat aggtctctat ttctcctttt
     7021 tcgggctcgg gaattcctgt agtaatagca aaataggtct ttttaatctc tcttctggca
     7081 aacatatcgg aaagctttgc ctgggtaaga tcatttttag ctattataat cagtcccgaa
     7141 gtacctctat ccaagcgatg aacaattccc ggacggttaa attcttttcc gctggaaagg
     7201 ttttctccaa agcgataaag aatggcattc accaaagttc cttcgggatt tccaaaacct
     7261 ggatgcacaa tcattcctgc ttttttattg ataatagcga ggtgttcatc ttcataaacc
     7321 acatccaagg gtatatcttg aggcaaaaca ttcatcggtt ccggctcggg aagattgata
     7381 ataacttcat ctccttcctt aagaagatag ctttttttta cgggagtaag attaaccaaa
     7441 acacgctctt cttctatgag gtgttcaata aaagtgcggg aataaagttc ctgtatcttc
     7501 aattccgcca gaaacttatc caagcgttgc atttcctgta aagtatagat taccttcagt
     7561 ttatctttca ttatcataaa attccagcac ataaacagcg ccggtagaaa gaccttttct
     7621 gtttctcact atggaaccat taattttaac cttgcccaaa tacactacat cttcaacaat
     7681 tatatcgtaa atattattgc catagcgcag ggaatctaag ataacggaaa aaatgcggtt
     7741 atggtaatcg tttttccaaa tcaattcatt caaatttacc gtttctgaaa tcatcggaaa
     7801 gcgttttctg aggggttcat tacagtagac aacatcacca ttagctccgg taattaaaat
     7861 actttgtggc agaaggttaa tcaaaagctg gatacgttga tggtgttcaa atattttatc
     7921 tgcctttgcc tgatcaaaac ggataattat tcccagcatt ttatccagag cataaatcaa
     7981 ttcctgaaaa tccttatcct ggtcataaat agagctatca atatcaatct gatagttgcc
     8041 attggaaatt tcttctatca gctgttccac ttcacgaatt gctttatgca ggcggaaagg
     8101 tatatagttg taaagaatta cgatatacac aaaaatgata aagacgaaga taatcacaat
     8161 tgaatgaata tcgttagcaa gagttgttaa gtcctgtgcc cgagctacag aaaaaagcag
     8221 ggcaagagtt tgtaacacta tgatgccaaa gagaatggca atcaaaatat ggtatcgtgc
     8281 tttaaaagag cgtttcattg tttctcaatc cttcttaacg gctgtttcat agatcagttt
     8341 atctatattg ccttttggtc ctaataaaat taaaacatcg ccttcgttga taatatcatt
     8401 ggctccaggc atatcattca gccgtttttc tattacattt tgaccgtctt ccgtaacaga
     8461 gagatagttg tatttgatag ctataacatt taccccgcgt tttgtaggaa gggcaagttc
     8521 ctgtaacggc ttacctacaa attctttagg agcaaccatt tccactacaa tatgaccgct
     8581 ggagagattg atatagtcca aaacattacg ggaaatcaag gttttagcca gctggttgcc
     8641 aactatttct tccggcaata aagtatgttg aactcccagc attttcagaa ttctgccatg
     8701 gagttccgat tcaattttgg catagataat tcctacgcct atttttttca ggattgcagc
     8761 agtgagaatg cattcttcaa tattgcttcc aattccgata ataacggcat ccgcttcatt
     8821 cagattttgc gatcttaaag ctgcttcatc ggtactatcc atacagacag cagaagtaac
     8881 tatagaactg atttcattta ccaaatcctg atctttatca atggcaatta cttccatacc
     8941 gttttctgtc aagattttgg caacagtcat tccgaaccgg cctaatccaa ttactgcata
     9001 tcttgccata tttttctcct tatttcaagt aggttaatct aaccgatgga aattctttct
     9061 tccgcataag atatatgggg ttgtcttttg cgaatagaaa tggcatatac cagggttaga
     9121 ggtccgattc ttccaatata cattaataaa gttatcagca gtttacccgc ataagataac
     9181 tgtgatgtta ttcccaaact taagccaact gttccaaaag cggaaaatgc ctcaaaaagt
     9241 attttctcaa aaggaaacgg ctcaatgagc atcagaataa aaataatcgt taaaataatc
     9301 gccaaagcta atgttgccaa agttgttgct tctcgggcat tagcaagaga tattttgcgc
     9361 ttgaaaacta ccagatccct ttttccggta agcatagaaa ggacagaaag cattaaagtg
     9421 gaaaatgttg tggtctttat tcctcctcct gtagaacctg gagaagcccc aataaacatc
     9481 aataaaacag ttaacagtac agacgcggaa ctgtatgaac caattttaat cgtgttaaag
     9541 ccagctgtcc ttgcagttac ggattgaaac caggaactta aaaccctgcc agcaaccgaa
     9601 aaacctttca ttgaattgtt atattcacct atataaagcc ccaaaaaacc tgtaactatc
     9661 aatattgcgg tagtggaaag aacaattttg gaatgcaaag ctaacttacg aaccctcctg
     9721 gaagaaaaga aataatgata aatatctatc aggacggcaa aacccaatcc gcctaaaata
     9781 atcagaaaag aaaaactaaa aattaccaaa ggagacccta caaaaccggc aagattatta
     9841 ctccacaaag aaaaacccgc attacaaaaa gccgatatag aatgaaacaa agaataatat
     9901 atcgcttgac ccgcaggcat tagtttagca aaagaggtaa aaagaaataa ggcacctgtt
     9961 atttctatta tcaaagtaac caacacggta cttctaagta atttcagaac atcaattaca
    10021 ggggtcttac ctacaacatt atgcatcaag tttgttacat ttaaagaaat tctttgcccc
    10081 agcattaaag caaaggcagt agatatagtc ataattccta aaccgccaac ttgaattaag
    10141 agtaagatta cgatttgacc aaacaaagaa taataagttc ctgtatctac aactatcaaa
    10201 cccgtaacac aagtggcaga tgtggctgta aataaagcat ctacaaagtt tgtaatataa
    10261 ccctcagtag atgaagcagg taacatcagc aaaattgttc caattaaaat aacgcctgca
    10321 aaactgaaca tcaaagaaac aggaggattc ctcatcagca atttgtatat tttaccctca
    10381 aaagctctga ataaaatgaa ctggacgata aaccaggtct gccggattaa aaggaaaaag
    10441 attacaaact ttgcctgata ggtgagcagt aatgaaccca aaatcaacat caccaaatca
    10501 aaatagacaa ccgttttgtt ttctttcacc tcccgggaaa gcaccctgga aagaatcgta
    10561 agcactaata gaactaaatt tgctagagca gttataaact caatagtaga ataggaactg
    10621 gtgtaatatt caaatatggg ctccagaaaa agaacgaaaa aactccagaa agccagtata
    10681 taggcaatta gttcaatgtg tttaaaagtt cctgatttct tctctgcggg cattttagtt
    10741 tattttgtta ccttattttc aggtcgttta ttatttcctg ttgaatctct tcaattgatt
    10801 tgcgggcaga tatttctttc agcaaaccct tttctgcaaa atattccttc aacgcgaagg
    10861 tctgttcata aaatgcctta actcgttccc gaattgcttc tccggaatca tccgtgcgct
    10921 gaactaaagg ggaaccgcat ttatcacaaa tcccttcctg ctttggtttt ttgcttaaaa
    10981 tattataggt ctcgccacaa gaactgcaaa gacggcgggc ttcaatgcgg gcaattgctt
    11041 cttcttcgct tagttccaaa tagtaaactc ttctcacatt ataatttttt aacagatatt
    11101 ccgcctgtgc caaagtgcgg ggaaaaccgt caaaaactac accagcacaa tcagctgtta
    11161 aagaagcatt tacaatttca aataccagtt cgtcctgaac cagctcacct ttggcaataa
    11221 ttgcctgcac ttttttcccg agttccgttt gtttatgaac ttcttcccgg aataaatcgc
    11281 caatattaat atgttgaaat ccggttcttt ctgccagaag ttccgcttga gttccttttc
    11341 cacttccctg gattcccaaa aagacgatta catctaacat tttttacctc attttttaca
    11401 aaaaaaagca attacattct tctgtcaagc gaaatgaaaa atagcaagag gttaaggggg
    11461 caagaggaca agaaaacaag aggacaagag ggtttttact aaatattttc acttgtcttc
    11521 caggaaaata ctcccccaga actaattcac ttacaaccga acttatatcg gtttacaaat
    11581 tgaaatattt tgctttataa tagaataagc cctcgtaatt tgtattaacc tataatgcat
    11641 acttacataa acaattagcc aaaaatatca tctcttccct aatagcattg cttacccatt
    11701 gcttacccat tacggatggc attagcaatg ggtgagcaat gctaaagtaa tgcacttagg
    11761 aaaaaatgag ttgttctttt aaataaatat aagtaatcag ataatttccc cttggaaaag
    11821 ctatagataa aaataccctt aaggattgca agtatctaat gtcatttttt gaatgataag
    11881 ctgtttgtca ttcttgcgca agcaggaatc cacacaagat aaataatatc tatgcgtttt
    11941 ccatccgata ttttataaaa accgatttta acccggtgta aggggagaaa aaccactcaa
    12001 ctacacttct atgaagaaaa aagttcccta ggaacgacag ataataacaa cgggtttcaa
    12061 cccggtgcaa agccatcaga aaagtcatta tctctttttc caagaaagag ttccgtagga
    12121 acaacagata ctaacaacgg gtttcaaccc ggtgcaaagc tatcaggaga gtcattatct
    12181 ctttttcctg caaaaagttc cgtagggaaa atacaatagc actcctttca tagtagatat
    12241 ggcaactgat ttagcagttt ctaattccgc ttaagtaact gacatttatt tttcagagtt
    12301 ttacgaattc tttggtaagt tggaatttgc taacccattt gtcctcaact gccttgtttg
    12361 gaataaaact actttgtctt acagaatttt taagacataa ttttccactt gaaaccgata
    12421 ttagatactg taaaaatatc ttttttacac aaaatgccta tttcagcaaa agtgccttaa
    12481 cggttttact ataatcgggt gcggcaagcc ggataaaata aactcccgag gcaaggtcct
    12541 ggttcttttc gtcctttgca gagaaatgaa attgctgttc tcccctgtta tagacggaat
    12601 ttataagagt tttcacttta cgtcctttga ggtcatataa ttctattaca atcggggtgt
    12661 tatccctata aatgctgaag gaaatagttg tttcaggatt gaaaggattg ggataaattc
    12721 ctttcaggga agtagaaata tgaggtgttt gaggatcttc aaccgcgttg ccttccaaat
    12781 taaaatcttc agtggtaaca attcccgccg taatgttcac ttgtctggta actgtattca
    12841 atccgggata ggaagctgtg atattatgca aaccactgct caataataag gaataagttc
    12901 ctgcggaaga ggtgtaagta gtttggctgc cgtcagccat aacagttgct ccaattacta
    12961 aatcagttcc atttctcacg gttcccctta aaaaacctgt attgggagga cgcataataa
    13021 atcccgtttg aggaaactga taagaatcaa aagcataaag tggaggtgct cccggatcgt
    13081 gttcctgctg gtcactgtaa atatattttg tgcgttgcat tgcgcttgat tgcatccgga
    13141 atacattgtt ggaattataa acattataat caaaaggacg ctgaaccatt aaaacaagat
    13201 tttgaccttg ataattgtaa gttggataaa gcgggaaaat tatttcattc tgtcccgccg
    13261 gaaaggacat tgttccataa taaaccattg ccagattaga agatggtatc caggttaaag
    13321 gcaaattgac aaggtctgtt tcacccagcc agataataac aggtttatca ttaatatttt
    13381 caataaaatt attgtaaaat atcaggtgcg ttataatccc cgttgtatct atttccgggg
    13441 cataaattag cgtctcaaat aaactggtgt gataactgaa atccaaaggc agattaccgc
    13501 tcagattacc tattcctatg gttaaggaga aaaatccggc ttcaaaagct gtaacattta
    13561 tgtgaggaga ttgattattg gtaagatcct gatcaccatt caacacaact tcggcaaaaa
    13621 tatcataagt gccaggagtt gtaggaaccc acattaatgt ttgaataaga gtttgtcctg
    13681 ctgaaacagg atttccgagg gcagtagcta aaagttctcc atcccctttt ttgaggttaa
    13741 caatgtaatt atcctgatct tcatagccat agttattaat cttcacctga acctgttccg
    13801 tagcacctaa agtaacaact ttatcagaaa taacttgcga acaggaaagg tcgctggcaa
    13861 tgctttgagc cgtgtaagta attcttattt taggatattg accattaagc acaaaaccgc
    13921 ttgcaggcgg attggcagga tctaataatt gagtatcatg ataggcattt cgggcacgaa
    13981 acatagtatc cgtctgacag taaaatttat cactggaaga aaaataacca ctatccatag
    14041 gacgttgcac catcaaaacc aaagtcccac atgtataatg aaacaatgta tccagctcta
    14101 tatcaattat atgaatacct gaaggataat taacctgccc gtcaaaaacc aaagttaact
    14161 ccgtagaagg aatccaaccg gcatttaagg tggtaagatt cgttgttccc aaccaaattt
    14221 tggtgggttt gttgtttagc aaagttacaa aattattgta aaaagcaatg cgggaaatag
    14281 ttccttgggc aaaacccaac tcattttgat aatataagca ctcaaataaa gagttttgat
    14341 agaagaaatc caaaggaatt cttgctgttt gagttcccga accgataatt acttcatcgg
    14401 cagttaaacc tgtccagata atggcgatac acgctattag cacgattatt ttggctatct
    14461 tgttcatcat aaactccctg tttcttaact gcaagtaatg aaattataaa gtaaatgaag
    14521 caacttttga accgtttttt gttataaaat tgaagaagca gaaatcacga ggtaatatca
    14581 tttttttgaa tgataagcaa tttgtcctac atccgaaagc agaaaccttt gtcagtcctg
    14641 cttcagcaga aatccacaca agataaataa tatctatgcg tttcccatac ttataaaaat
    14701 agatttcaac ccggagagca aaaatacaat atattgtact ttaaaaagtt ccataggaac
    14761 gacagatact atagaagggt tttaacctga cgaaaaatat atctacgcaa ggaaaaaagt
    14821 tccttaggaa cgacagaatt atgattttct ctgataaatt ttcatttttt ctggctaatg
    14881 acttccttct ctacataagt ccaatctatt ttattgtatc tttacctgct ggatcttttg
    14941 ctttttctcc tagttgcttt atgcgggttg aaacccgcta ttagtatctg tcgttcctaa
    15001 ggaacttttt attatagtgg taaagtttct cccattttca ccgggttaaa acccgttgtt
    15061 agtatctgcc atccctaacg ggattttttt agatttcaat aaggggattt caatctggtg
    15121 caaaagaatg aatgatcaat ctatcttttt cctctacaaa cgagttccgt aggaacgaca
    15181 ggttctaaca acgggtttta acccggtgat aagataagaa cctacaaaac tatatttatt
    15241 ggggaaaaaa gttccgtagg aacgacagat attaacaacg ggtttcaacc cggtgaaaag
    15301 caatcagaaa agtcattatc tctttttcca agaaagagtt ccgtaggaac ttttttccca
    15361 aagaagtatg gttttgtgga ttattctcta tgcctcaact aaacttttat tctccttcca
    15421 aaataacaaa agccaacgca tatccctttt catgagccaa actaagacaa atattattta
    15481 cctttagctt atctgcaagg tcttttgcac ctcctgttag attgatagtc ggagaaccct
    15541 tttctgaagt tacaacctct atatctatcc agttaacttc tccattccaa ccggttccta
    15601 atgccttcat cactgcttcc ttggcagcaa aacgaactgc aaaggactga taggaatttg
    15661 ctttgccaga acaatactca atttccgaag cagtataaag cttttcacaa aaacgggaat
    15721 ttttttctat agcattttga atacgttcta cttcaataat atcacaacca ataccaataa
    15781 tcattacaga ttgctccttt ccagttccag caactttttc ttgatagcga taccggctcc
    15841 ataacctgtt aattttccct tggaaccgat tactctgtga caaggtataa gaataggaac
    15901 cggattttta ttcagtgctt tgcccacagc cctgccggca ttcggattag caatgcgctc
    15961 tgctatttcc ttataagtta atgtgttacc ataaggtatc cgcatcactt cttcccaaac
    16021 cataagctga aaaacagtgc cggttgcaaa aaaaggaagg tcaaactctt tgctcctacc
    16081 tgtaaaataa aggtcaaatt ggcggaatat ttctctttcc aggtcattgg aaggttggct
    16141 ggaaacttga gccggacaaa aattgatgct cataatggtc tcttcctcta tttccagcag
    16201 cagatggaaa aagccatccg gactattgta aaatactctt tctgacatat aaaactcctt
    16261 taagtcagga gtcattagcg ccttaataat ttttccctta cagatttctt tgaagacgct
    16321 tataactcgt gacgacttat tatctctcac aaattacata gataatatag atttattaac
    16381 tttatcgtta cgagtcctca ttgctaaatg agtttgtagg taaagtataa tttatttcag
    16441 cgataatgac tcctcacttt tcttattatc attttcgcct caatttattt tcattcttcc
    16501 ttaaaaagtg gagggatggc gtactccggc tacacaaaac aaagcccctt cctaaaattt
    16561 aagaaggggc ttaagattga gtttttctgt gtaactatac agtgaatctt cttttttctt
    16621 tgattcttcc tgctttacct tgagcgctac gcagataaaa tagtttcgct cttcttactt
    16681 gtccatgacg aataatctca agtttatcaa tatttggaga attttgagga aaaatcctct
    16741 ccactccaac accactggag accttacgca cggtaaatgt tttagaaata cctgcgcctc
    16801 gtttttgaat aacgataccc tgaaaaacct gaatgcgttc tttgttacct tctttaattt
    16861 tataatgaac tttaacggta tcaccaacgc gataatccgg taagtcagtt ctaatttggt
    16921 ctctgctaac ttgttgcaga atatccattt gggttcctcc catatatata ctcggaaggt
    16981 aaaaaaccgt atgcaaacaa ggattaaacg gattaaaaaa aggagttttc tctatcccgt
    17041 tctaatattt atttacttca ttaagagcat ttctgtcata aaataaagcc ggaaattaat
    17101 aaattttccc taccggcaaa ttccttgctc acattagttg ttttaggttt ctttcttcct
    17161 gcttgtttgt ttactttttc aaatctggtc tgcgggttac tgttaagcgt tttccctgtt
    17221 ccaatgccca tttctttatt tctttatgat ttccgctaag caaaacttcc ggaacgccta
    17281 agctcataaa aatttccggt cttgtataac agggaaaacc aagcccttca ttactaaaag
    17341 aatcactatt ggcactttcc ctatcactca gcactcctgg cagtaatcta cttactccat
    17401 caataaaagc cagagccgga atttcgcctc cggtcaaaac ataatcccct aaagatattt
    17461 catctgaaac acagagatca cgcactcttt gatcaatttc cttatagtga ccgcaaagca
    17521 ggattatgcg tttataggaa gagtaactct ctaaaatctt ttgagttaaa ggtctgcctt
    17581 gcggagtgaa ataaacaact ggagcatttc ctatctctaa aagttctatt aaagcactat
    17641 aaatcggttg tgcctgaata accattccgg caaaaccacc ataaggataa tcatcaactt
    17701 tatggtgttt atttagagaa aactgacgat aatccgtcaa atttacttcc agcagttttt
    17761 tctctctggc tcgggcaata atgctgctgt ttaaaaaccc gctaaaggca tcgggaaaaa
    17821 gggagaggac ttcaaagatc atttcttttt tgcttctact ttataaagag caacaaggtc
    17881 tacagcattt tgcagaatca cgcaacgcag attatcaatt accgtatcta tataataatc
    17941 cacaaaaggg accaggtatt ccgttccctt gctatccaca atctgcaaaa catcctgcgc
    18001 tccgttgaaa aaaaagtcct ctacaattcc caattcctca cccgcaaata taacctggta
    18061 acctataaga taattaagag atgtttcttc cgtctctatt gccgtgtcat ctatggcaat
    18121 cactacatct ttgtgtagaa attgttcttc ggaaacacca tcttccttca gttttatcca
    18181 ggttttatta tctttccgca ttctctcact gatagttaca taaaacactc tatcgctgtt
    18241 aaagatcaaa tacagatcct caagctcaga aaacacgctt ctgtattccg gcttgatcat
    18301 cacaggatga aagccatctg cattctgcct acccagtttg ccgattccga taagaggggg
    18361 atgtttcatt tactcgataa tttcgagctc ggcacgcttc ccctgttttg tgctaacagc
    18421 attaataatg gttctgatgg cacttgcggt tctaccgcgt ttgccgatta cttttccaat
    18481 gtctgctttg gaaaccctaa gctcataaag ggtaattttg tcgccagtga tctcggtaat
    18541 attcacttcc gaaggatcat caaccagagc tttaacaatg aattcaatga gatctttcat
    18601 ttttcacctc agttttcttt tattctttat tctgcttccg cagttttctg gttcttcatt
    18661 tgtttattat tatgccaaat ttgcaagaca cctgctttgc gtaacagaga acgaactgta
    18721 tctgatggct gtgcaccaac gccaagccaa taaagagcac gttctgtatc tatttttatt
    18781 ttggaaggat cgggcttggg atcataccag ccaatgcatt caatatattt gccgtcacgc
    18841 tttttgcgtg aatcaactgc tacaattcgg taaaagggct gatttatagc acccatcctt
    18901 ctcaatctta attttaccat tcattctcct tatcattatg aacataatta ttttcaattt
    18961 tattcttaga ttttagaatg cttatttccg tcaagataaa ttttttatgc ctttactgaa
    19021 aaagctttgc actgttttag cgacgaccaa ttccataata agtaaaaccc atttccttaa
    19081 ccgctttagg attatattga ttccgaccgt caaaaatcac tttctgccgc attattttac
    19141 tcatcctttc aaaatcggga tagcgaaact gatgccattc cgttatcagt aacattgcca
    19201 ctgcatcttt cagggtttca tattcatcgg cacaaagagt tacggaatta ttattgccga
    19261 aaagcttttt cgcttcctgc atagctacag gatcataagc tttaaccgta gcacccatag
    19321 caataagttc attgattatt acaacggaag gagcttcacg catatcatcc gtttgcggtt
    19381 tgaaagccaa gccccaaacg gcaaaggtct tacctttcaa gtttttgcca aaatgagctt
    19441 tcactttttc caccaagacc ttcttctgtt cggagttaac ttcttccacc gcttttaaaa
    19501 tacgcggttc ataaccaact ttacgagaca tattgataag tgctttgatg tccttaggaa
    19561 aacaacttcc cccataaccc actccgggaa aaataaattt atagccaatg cgggaatcac
    19621 tgcacattcc atttctcact tctgcaacat ctgcatcgta ggcatcgcaa agacgggaga
    19681 tttcgttgat gaaagatatt tttgtagcca gaaaagaatt tgccgcatac tttgacattt
    19741 ctgctgaacg gatgcccatt atcaaaatgc ggtcatttgt tctgcagaag ggttcataga
    19801 gggttctcat aatttctccg gctttaggat tatccgttcc aatcacaact ctatccggac
    19861 tcataaaatc atctatggca gagccctctt tcaggaattc cggattggac acaacagcat
    19921 aatttatatc cacaccacgc gcattaagca cttcctgaat tttttcattt accaaatctg
    19981 ccgttccaac cggaactgtg gatttattaa cgataatttt gggttcattc aggtaggaag
    20041 caatttcttc tgcagcagca ataacatatt gtaaatcagc agaaccgtcc tctccgggag
    20101 gagttcctac agctataaaa ataatttgtg aatcctgcac tgcctgcttt gtatctaatg
    20161 taaaattaag acgcttggct gtcatattgc gcagaatcat atcttccaaa cccggctcgt
    20221 aaataggaac tttgcctgct tgaagcattt ctattttgtt ttcatcctta tctacactga
    20281 ttaccgtatt tcccatttct gcaaaacaag cacttgtagt taagcccaca tagccacttc
    20341 ctataactgc tattttcatt gtagctcctt aaaatatttt ataacttggg aaatgccttc
    20401 gctaagacct gtttccggtt tccagcccaa gacctttttt gccttttcac aggaaagcag
    20461 ggaacgatgt aaatctccct ttctggcagg tccataatag ggctcacggt ttattcccaa
    20521 ttgttttgct attgcgtaat ataaatcttt agtagtggtt ggaatgcagg ttccaatatt
    20581 gaaaacttca ccctctcccc tttccagagc cagtaaattg gcttgaacaa catcccccac
    20641 ataaacatag tcccggatca ttccatccgg ctcattatca taacgataga cagttggtat
    20701 ttcacctttc agcagttttt ccgtaaaaag agagacgact cctgcctctc cctgggatat
    20761 ttgtcttggt ccgtaaacat tagcataacg caaaaccgtg taattcaagc catattgatg
    20821 acggtaaaaa tagaggtagt cctctcctac gcttttattt atcgcataaa tggaaagggg
    20881 cttgggattg tatttttcag atgtaggata ctcttccgcc tcaccataaa tagctccacc
    20941 tgaagaaata tagataactt ttttaacccc tgtcctaacg caattttgca acacattcac
    21001 ccagctataa acattgttta ttacatccag aaggggattt tcaatggaga gcggaacgct
    21061 tatttgagcc gaatgatgat ttaccatatc gggcttttct tttgcaaaaa cctcgcttaa
    21121 actttcatca cagatatcca tttcgtaaaa aatcgcttta gggttgatgt tacgcctgtt
    21181 gccagttact aaattatcta caataacaac ttcgtgccct gcttgaagat aggcatctgc
    21241 tacattggaa ccgataaaac ctgctccgcc tgttactaaa atcttcattc attaatctcc
    21301 acaatttcaa attcaccaat aatcttgatg tcttgtgttc taactttaac cagcactgcc
    21361 tgctttaaaa tatttacctg caaacgcact tccgtgagtt tggattggtc ttcaactaca
    21421 cctctgatgc ctttcaaggg accgtttata atttccactt ccaaaccctt ttccagccaa
    21481 agtccgggtt ccatttgcac atttttctgc cttccataat agattgcctg taattcatca
    21541 atcagttctt tttgagcatt caccttcaga aaattgacgg tataacccga aatcaataat
    21601 tcctgtttac ccttaaaatc aatccctaca aatacataag agggaaaaag cactttattc
    21661 acatctactt tgcgtctttg ataaacccgc ttggaagtat attgtggcag gtaataagtt
    21721 atctgatgct gttttgcata atcggcaagt ttcttttcac agcgtggttt ggtatgcact
    21781 acaacccatt tcctgtcatc tgtgaaaagc gcaagttcac cgtaaagttc atctacatag
    21841 acaggtttat gcgtagccat ttttaatagc gataataatc ctgtttatag ggtccttcaa
    21901 taggcacatt aatatattct gcctgttttt tggagagctg agttaattgt gctcccagct
    21961 tgggaagatg taagcgtgct acttcttcat ccaatttctt aggcaaagtg taaacattta
    22021 tctcgtgttc attctgccag agttctattt gtgccaaaac ctgattactg aaagaacagc
    22081 tcatcacaaa ggaaggatga cctgtagcat tacccaaatt taccagccga ccttccgaaa
    22141 gcaaaataat tgctttatca ttgggaagtt tgattaagtc aacttgtggc ttgatattca
    22201 ccttttccac accttccatc tcatagagtt tatttacctg aatttcattg tcaaaatggc
    22261 ctatgttaca caaaatagca ttgtttttca tcttcaaaat atggtcaacc cgaataacat
    22321 cacaatttcc tgttgccgtt ataaaaatat ccgcaatttc taccatattt tctacagtat
    22381 tcacttcgta accttccatc gctgcctgta atgcacaaat agggtctatt tcactgatta
    22441 caactctggc acccaaacca cgcattgatt gtgcacagcc ctttcctaca tctccataac
    22501 cgcaaatcat aacaattttg ccggcaacca taatatccgt tcctctcttt atgccatccg
    22561 cgagggattc tctacagccg taaagattat caaacttaga tttagtaacc gaatcgttta
    22621 cattgatagc gggaaataac aatgtgcctt catttcttct ttgataaagg cgatgaactc
    22681 cagtggttgt ttcctcacta actccgcgta agcatttcac tgctctatgc catttgtaag
    22741 ggtctatagt taattgattg ataagcaatt cctgcactat tctcatttct gcattctcgg
    22801 catcaacttc gggcagtttt cctgtttgtt tatactgttc ttccaaacgg tatccttcat
    22861 gaaccatcaa agttgcatcc ccaccatcat ctacaattaa atcgggtcct tcttcccatg
    22921 aaagtgcctg taatgtacac caccagtaat cattcagagt ttctcctttc caggcaaaaa
    22981 cggggattcc tctttgggca atagctgctg cagcattatc ctgagtagaa aagatattac
    23041 agcttgccca acgcacttga gctcccagtt ctaccaatgt ttcaattaaa acagcggtct
    23101 gaattgtcat atgcaagctg ccggttattt tggctcctgc caggggttta cgatgacgat
    23161 agcgttcttt taaagctacc agaccgggca tttctgcctc tgccaaatca atttcttttc
    23221 tgccccagtc cgctaaactc aaatcggcta ttttgtaatc cattttttcc tctatttatt
    23281 ttttttaacg atgatgacga tgatagtcat tcctaccgct atttctacct ctattcctat
    23341 catcacgatt ttgctgatga taattttgct gttcttctct atgggcatca ggatgtggtt
    23401 caggatttcc agcaactcct ttcatagata aagaaagttt gcctttatcc attcccaggg
    23461 ttcttacttg cactttatct cctaatttaa ccatatccag gggatgattt atgcggccgg
    23521 tatacatttc cgaaatatgc accattcctt ctttcgttcc ccctaaaatt tttacaaaaa
    23581 ttccgtaggg ttcaatgcgg gttacctgac cttcaaacat atcaccggca acgggatcaa
    23641 cagcaattcc tttaattata cttttggctt tattaatgga ttctttatcg ggagaaagta
    23701 ttctcaccgt tccgtcatcc tgaatatcaa tctgtacctg acattcttct ataattgatt
    23761 ttatcatctt tccgccaggt cctataatct ctccaatctt atctataggc actttgaagg
    23821 attcaattcg gggagcccaa ggagctaagt cacttctggg ttcggcaatg cattcagcca
    23881 taatatccaa aatcttaaag cgagctgatt ttgccttatt taaagcaatt tgcataattt
    23941 ctctggtaat tcccgaaatc ttaatatcca tttgcattgc tgttatacct tcccgcgttc
    24001 cggcacactt gaaatccata tctcccaagt gatcttccaa acccataata tcggttaaaa
    24061 caacatagtc ctcaccttcc attattaaac ccatagcgat tccggcaaca ggagctttta
    24121 taggaactcc acctgccatc aaagctaaac atccgctaca aatagatgcc atagatgaag
    24181 aaccgttaga ttccagtgta tctgccacta tgcgaatagt gtaaggaaag gaatctttat
    24241 cgggcaatac agcttttaaa gccctttctg ctaaatttcc atgtcccagt tcacgacgac
    24301 ccgtaggtcc tattcttccg acttcgccaa cgctataggg gggaaaattg taatgcagat
    24361 aaaagctctt cttatattct gtttccaagc cgtctatcac ctgttcatca gttcctccgc
    24421 ctaaagttaa agtccccagg gattgtgttt cacctctggt aaaaagtgcc gatccatgca
    24481 ctcggggtaa aacatcaatt tcacaagtaa tttcccgaat gtcatctgtt ccgcgaccat
    24541 ctacacgatg ctgttttctt aaaatggaat cacgcacata gcgacggata agctcatcaa
    24601 aagccatttt atagtatctc tccgtttctt cccaagggct taaatccgta tcttcttgct
    24661 gggaaaacat ttctaccatt tctgcttcag ctgcgtcaaa ggcatcctgt ctttcctgtt
    24721 tgccaaaaat aacggctgct tcagctattt ttgcaccgaa ggcattttcc accttgttca
    24781 ttatttcttc cggaacgctg tccagaacta cttctacctt ttcttttccg caggcagaaa
    24841 taaaatcctg ttgcaaagcg cagagttttt gaatttcctt atggccagta aaaacagcat
    24901 ccagcatcat ttcttcgctc agttccgaag ctgctgattc aatcatcaca attgaactct
    24961 cacttccggc aacggttaaa ttgagcttgg attgggtttc aatttcatta aaatggggat
    25021 ttactacaaa ttggttatca atgtagccaa ccgtcacact ggcaataggt ccgttgaaag
    25081 gtatatcaga aatggaaaga gccaaagaag ctccaaaaac acccattgta gaaggatcgg
    25141 ttactccatc aaaagaaagc acggttacta caatatgaac ctgattccga aaaccttccg
    25201 gaaacaaagg acgaatggaa cggtcaataa cccttgccat caaagtggca tcagttgagg
    25261 gtttggactc tcgtttaaaa aaaccgccgg gtattttgcc ggctgcatac atcttttcaa
    25321 tgtaatcaac agttaaggga aagaaatctt gatcttccgc gggctcttta gacatcgtgg
    25381 ctgtaaccaa aacagctgtt tctccgtatt gaataaaaac actgccattg gcttgctttg
    25441 ccattttgcc tgtttctaca atcaatttgc gtccgcaaaa atcggtttca cgacgagtaa
    25501 tattgaagtt atgcataact tctccttttt tatctttagc gggagaagaa gcaataggcg
    25561 aaaaggtctt gaccctaaac cttttagctt attgctaatt actcccgcct tttttacagg
    25621 aaggttgctt ctggctaatc ctttaagtaa ggagtcatca ctgcctcaat aacttttacc
    25681 aaatttattt ggcagtgagg actcataact atttcaaaaa tccggaaggt tgacctcagg
    25741 gtcaaccatt aaacaacaat atgtcattct tccggaatta gctacaccaa agtttatttc
    25801 cttatgccga gcgtcttgat taaattacga tatctggtaa tatctttctt ttttaaataa
    25861 tcaaggagac gcctgcgttg cccgatcagt ttcaacaagc cgcgccgggt agaaaagtcc
    25921 ttaggatgtt ctttcaggtg ctctgttata tcttttattc tttgtgttaa cagagcaacc
    25981 tgaacttccg gcgagcccgt gtctgattca tggagtttaa actctgccat gatagcttgt
    26041 tttgcctcag gttttaacgg catttcatcc tccagatgat tttgagaacc atattttaga
    26101 gattagtata attgtccact gtaatttttt gtagcgaggt caaaaagttg gttatatgca
    26161 tatacataag tgattattta taagtgtcta atagtataat aaactgaaaa tcacttcatc
    26221 aattaccaat tagttttctt ttcctcttgt tctctttgtt aacaatctaa attaactctt
    26281 gagtaaattc attcttcatt agcagaattt tggtggaggt ggcgggaatc gaacccgcgt
    26341 ccagaaaacc gatcacccga aaatctacat acttagttgt ctttatctta agaaaagcag
    26401 tgaaagacaa tcagacctgc tttcccgcag ccgattaaac ttaggtaaaa cttatcagca
    26461 atcagttcta ccgcatccgt actgttccga cgcccaatct ccttccgacg gaccgaagaa
    26521 gttgaacgtt aacagcctat gctgctaaag cgtaattgtt gtttgcagtt aatttgcagt
    26581 ttgttacttg ttaacggagt ggtataacaa tctccggtat gcatttcgag cactctgatt
    26641 ccctgtcgaa accatttcac cccctataaa ctgtataaaa gaactataaa tggataaatg
    26701 tatttgccct gcaaaaatct cttactataa ccataataat caaaatacct ttgctgtcaa
    26761 cctgaatttt tggagttgat gagacaaccc cgacagatat tatagaaggg ttttaaccct
    26821 ttgataagaa aagaacctac aaaactatat ttattgggga aaaagttccg taggaacgac
    26881 agatattaac aacgggtttc aacccggtga aaagaaaaga acctacaaaa ctatctttat
    26941 tggggaaaaa agttccgtag gaacgacaga tattatcaac gggtctcaac ccggtgataa
    27001 gagaagaacc tataaaactg tatttctttt tggacgaaag ttccgtagga acgacagatc
    27061 atagagaggg ttttaaccct ggaacctttt ttaactcaca attttttaat tggagattca
    27121 gttattgctc ggttgaaagt gaaataatta gtggatagtg cttttctgga aggcaagtga
    27181 aaattgtgag tcaaaaaagg caggttgaaa ttgcaataat tagtgaatgg tggttttctg
    27241 gaaagtaagt gaaaattgta agtcaaaaaa ggaagaaacc gattttgctt gacagtatat
    27301 aatgatttat aatatctgaa aacaataaaa gaaagggagg atagaatgaa gatatccgca
    27361 attttctacg ctattgtaat tcttgcgttt tttatgccgt tttttatggt cagttgtgat
    27421 cagacagaag ttgcgtcttt aagtggcgtc aaattagtta taggtggtga agtaaaactt
    27481 tctatagcag aagagataga aaaaatagag gatgtaggta aaaaatatca aaatcctgtt
    27541 gtaaatattc agccctttgc tttaatagca ttatttatag ctgtattggc attagttttg
    27601 gttttggttc ttcctatcca atactattta ctacctgcat atattagtat tataggtgtc
    27661 ctttctctcc agttgttaaa tgtaggaata ctgcaaatta taactgctta tcagccgctt
    27721 tttgatcctt ccatagattt gaacagcatt ctgattatta aagcgcagcc ggctttttgg
    27781 ctggcaaatt tggcttttat tttagggggt atttttactc tgattgcagg tgtgaaaagt
    27841 agctctatat cggcaccagc acaagaaccg gaggaagttc aagaggaata tattacttct
    27901 gaaattcagg aatgggtgga ggaatatcct tcagaagaaa cattttctga ctcggatgaa
    27961 gaaaatttgc cagcggaaga agagaattct gttcttatag aagaaaaaaa tacacctgag
    28021 taaatagggc atatatggat agtgcaaaag agctgcgctg gataagaata ttattgctta
    28081 tcatcgcttt agccgttgtg gcttctattc tcaaaacgct taaaagtatc tttattccct
    28141 taatcttttc tatttttctg ctttttcttt ttgctccgct gattaactat ctgaagaagc
    28201 ataaagttcc aatggtctta atcttgctga ttactttagt gataatagct gtctttttag
    28261 ggttagtaat attgctaatt tatgctgctt ccaatagttt aatcaatggg ttgcctcgtt
    28321 atgaagataa atttaatgaa ctaatagcca aaggaacaga atatcttcaa aaccttgcat
    28381 ctaactggaa tatcaatacg gagaatattt ccattgctaa tatagcacaa attataagca
    28441 gcggatttat ttccattcct caatttatca gcaacacggt gaacactctg gtaagtatca
    28501 ttcagaatat ctttttaata atcttttttc ttatcttttt gcttttggag attgaaaaat
    28561 tgccattgcg attaagaaaa gctacctcca aattaagcaa agagcagaca ttggatatcc
    28621 tgcaaaacat agaaaaacaa attcagaact atttaactat cagaacctta gtaaatttat
    28681 ctgccgcgct tctgtgtatg ctgtggatgc ttattttcgg cgtggatttt attcttgtct
    28741 gcggaatttt gctttttgtc ttggatttta ttcccgatgt aggttcagtt atttcttccg
    28801 caattcccat acttatttac ctgctgcaga gtggtttcag ttttttatgg ttaatatttg
    28861 ccctattgat tgttgccact caaatgctga ttggcaatat aattgagccc aagttacagg
    28921 gtgtgcagtt aaatctaact cccataatgg tattggtttc tctcattttc tggggttggc
    28981 tttggggaat tgtaggaatg ctgatttgtg tgccattgac ttctgctatc aatattattc
    29041 tgaaacaggt tgcaccaaat aattttattt ccactctgat cagcagcgaa taatattttt
    29101 tttaagcttg ctttaccctg taaagattat gtaacaattg agtaacgact ccgtaacgag
    29161 ataagggcag tgttacggaa gtgttacgga agtgttacgg ggttgacttg gaacgaacta
    29221 atttttaaac aattaggtgt tcaaattgaa gacggagatg atgatgtgtt tcatatcatt
    29281 ctaagtattt acacagcaga aagatagaat gttttctcca gcttttaggc aggaacatat
    29341 aaaactcaac tgggcacaac ccaaaaagag aaaataaatt acttcctgta gttatctgcc
    29401 ggaagtgtgc aagagctttg tctttttatt tataaaccta attatgagat aaaataaaca
    29461 gctcgtaata aaaatgattt tccaagcagg atcaaagaca aatctttgcc taataataag
    29521 atcactaatt atttccagag caaaaagtga gcagatgaga gcaaaaaatg tttgatattc
    29581 attgcggata acttttttcc aggaaaaagg aaattcgggt tttttataat gactgaattt
    29641 agggaaatac acaggtgttt tacttgccca agatacaaat tcctctccgt atttcttccg
    29701 gagaaaagct tcttccgcaa agatcacccg ctcataataa agagtaaaaa tgagcggata
    29761 aattatgata acccaaatat gccttgtaaa taacaccacc cctaaaataa tgaaaaaatt
    29821 accaaaatat aagggatgcc tgagaagaga atacataccg gtagtattta atttatcggc
    29881 aacctgactt ttagtatttc tcccggaagt tccggaaggg gtatgaccaa tagtaagtac
    29941 tctaattgcg atgccacaac cgcttataga aaagcagata acttctatta tcgtatcgta
    30001 ttttatgaat tggctatttt ttaagtccag aatagctcca ataccgagaa taaacattaa
    30061 aagtggaaga taacttctcc acttgaatat ccaagcccct gatttttcaa attcttcttg
    30121 gatgggcatt ttcactccta aatttgtttt atttggtaca gatatatata aacttatcta
    30181 aagtaagaga taatatatgg tagttatccc ttttcattac aattatagat ttaagcattt
    30241 tcccttccaa tttaattcca tcttccatat tttcatctca gtaattatca ttttcataat
    30301 cgcttttaat tccacttttc tctatgttaa aaataagttt gctgaattcg gtgctcatag
    30361 tggctaaaac tgtttctgcg gtttaaccct aaaaatgaaa tacagcgttc ccagcaaacc
    30421 aaaagccgaa gcagttaaga aatttaattg cggacttttt tgccacagga aagctcctcc
    30481 aaatgctgca aaagcaacaa ttacatcccg aatcagatag taaactccaa aagtatatgc
    30541 cttagcattt tccggagcta aatccataat caatgccttt cgtgtcggtt ctccaaattc
    30601 tttcaagccc cgaataataa aggcaattac cattgcagga aaactatgac tgaagagaag
    30661 aactaaagga aaaatagtaa aattaacaaa agtaattgct acataaggtt tgcgagtgct
    30721 tctatctgcc agataagcaa ccggaatata aatcagcatt gctgttacca tttcaatagt
    30781 agttaaaata ccaaactgca attcgttaat atgattattt tctactaccc aaacaactac
    30841 aaaggcataa ggaatctgtt cacaaaaacg caccaggata tcggaaacaa gtaaattacg
    30901 caaattggca gaaaaaagac ccctcccttt ttttctttcg gttttcgctt ccggaggttg
    30961 ctcttgaata aatttatcct gaataaataa agccactcct gctaaaataa aagccactat
    31021 aaaggcataa cgcacaccct ggatgcgtcc gtaatgtaaa attaaggcac cacctaaaat
    31081 cggacctaaa gacattggaa tccgctttac aatagaattc agagaaattc ccatagagcg
    31141 tctgttggca ggaactacgg aattaatcaa actcatcgtt gccggtaaag aaatagctgt
    31201 ccaggaaata aaaaatacag cccccaccaa aactgctatc caggaaggaa aaatgaccac
    31261 gattaaatag cccaacatag ccattaagtt aaaaatcttt aatgcttttt tgtaacctat
    31321 tctatccgct aaccaacctc ccggaaaaga atataaagca cttaagagat tgtccattcc
    31381 atttaagaga ccaataacta tagaactgcc acccatagca ataagataca aaggcaaaaa
    31441 gcgttccgcc attttttcgc ccatccctac aagaataacc ataatcaaca ttcccagcat
    31501 actgctgttg agagcgaaaa aatggattag ttttctgaat atgttttttc tttcccgctt
    31561 attattaatt tcggacatta aaaaagctcc tgacaactgc aaaacatctt cattatatta
    31621 acaatataag gcattattaa tccgcagtca aattttttta accctttgaa ataaacccaa
    31681 accctgaata aataaaagca cgcggatatg gctgattttt tatgacttac tgtattttta
    31741 actcctaccg ttacaagtcc tcactgccaa attaaataga aaaaatgata aaattgttaa
    31801 aggcagtgac tactccttac ttaattcctt ctccatttgc attttccttc tctttcactt
    31861 tggacttgac agaaaaataa atataaaaaa taatggaaaa aggagaatgt ttatgattga
    31921 agtgttaatt gaaaaatgcg tcggttgcgg tgcttgtttg cgtgcatgtg cttatgatgc
    31981 cataaaaatt gaagaaaagc ttgctattat tgacagtgat aaatgtgttt tatgtggtgc
    32041 ctgtgtttca gcttgtcctt ttgatgctat tttattacgg aaagccaaaa cggaagcaat
    32101 tgacaaaagt gaatataatg gaatttgggt ttttgccgaa caaagggaag gcgttattgc
    32161 tcctgtagtt tttgaacttt taggaaaggg tcgtgaactt gccgatgagc ttggttcaga
    32221 actttcggct gttcttttag gacataatat tgaaggtctt tcttccgagc ttatagcttt
    32281 tggagcagat caggttatag aaattgatga tcctgcatta aaacattttt gggatgaggg
    32341 ttatgcttat gctcttgccg aacttgcttt gaagtataaa ccctctatta ttcttgccgg
    32401 agcaactgtt atcggaagaa gttttattcc tcgggtggca attcgtttac atactggctt
    32461 aactgctgat tgtacaggac tttccattga tccggaaaca ggagatttat tacaaacccg
    32521 tcctgctttc gggggcaata ttatggctac aattaaaaca gctaatcatc gtccacaaat
    32581 ggctacagtg cgtcataaag ttatgaatcc cattcccaga gatgatactc gcagtggaat
    32641 tgtaattcag gaacagataa attttgattt ggatgaggaa agaactacct ggcttggttt
    32701 tgaaaaggaa aaaaccggtt taattaatat cactgaagcc aatatcattg tctccggtgg
    32761 aaggggcttg aaagaagcaa agaattttgc tataattgaa gaactggctg aggcattagg
    32821 aggagcagtt ggagcttcca gagctgctgt tgatgcagaa tggattgctt attcgcatca
    32881 ggttggacag acaggaaaga cagttaaacc ccttatttat attgcagttg gaataagtgg
    32941 tgcaattcag caccttgcag gaatgtccag ttccgattat attatcgcta tcaacaaaga
    33001 tcccgatgct cctatcttca aagttgcgga tttaggcatt gtaggtgatc tttttgatat
    33061 tgttcccaaa ttaacaaaaa ggataaaaga acaacgagta tgataattaa gcccgcgatc
    33121 agtgttatca gccctgacag aatggagcag ggaattgatt tatatctttg gggaagtcct
    33181 tttgaggaaa tagacctgcg caaggattgt tccaatgcca ttgcctatat tcccttaatt
    33241 aattctcatg accatttaat cagtaactgg gttccccgag caggaaacaa ccgtccctac
    33301 aaaaattctc atatctgggt tgaagatatg aaggaatcct ttgccttcca ggaaagggat
    33361 attttttggc ataataacgg tagttttcgt ttggaagagc ccaattcgct tattttggct
    33421 aagctgggtg cctataaaag cttattttgc ggatgcagtg tagttcagga ccattcatcc
    33481 aatcaggtgg atgcctatta tgaagcaatg ccaattgttg tacctaaaaa tttccgtcag
    33541 tgtcattctt taactcttgg taactggtgg ggaggtgaaa ctccggaaaa agaaatggaa
    33601 ttgactaaag ggaaaatgcc ttttatagtt cacttgggtg aaggaaaaga cgaaattaca
    33661 aaaggggaat tttcccagct gaaaaaaatg ggattgttga gagcaaatac tttaattatt
    33721 catggcattg ccttaactgc agaagaaatt agtgaagtag caaatgttgg agcttctatt
    33781 tgctggtgtc cttcttccaa ttttttcctt atcggagaaa cactaaagat tgatgaagtt
    33841 cttaagcata aggcaaatgt atgtattgga acggattcca ctatgtccgg aggagtgaat
    33901 ctgattgctg aattccttaa aatcagagag cattttccca aaattcctac caaagaaatc
    33961 tatcggatgg ctaccgttaa tgcagcaaca gcactttatc tacctcaata ttttgctttt
    34021 attattccta ataaatgccg taatctattg ctggtggata aagttgaacg cgatccctat
    34081 gaaaaccttt ttgccttgga agcatcaacc atacagcttt tattggtaga aggaataccc
    34141 cgtttcggag atagtgaatg gatggaaatt ttaaatttgg atgaaaatat gtatagcacc
    34201 ttccgcactg gaaacaagga gaaattcgtg attggtgacc ctatggaatt gaatgatcag
    34261 attgatgagg ttttggggta tcataaggat tttccgtatt tgccttttta ggttgagagg
    34321 tcttgtggaa atggagaatg aatgaggagt ttgaaaaact tcttatttat tcaaagctgt
    34381 gtaatctgtg taatctgtgt aatctgtgta atctgtgaga gaatattatc gggttaaaag
    34441 atggaaaaac acttgaatgt aacagagatt tactattctt tacagggaga atctgcctac
    34501 agtggctatc cctgtatttt tatccgtctt tccgaatgta acttacgctg tgtttactgt
    34561 gatacccaat atgccttcgg taagggaaaa tcaatggcta tcagttccat aatggaagaa
    34621 gtgaagaaat atccttgttc tctgacggaa attactggag gagagccatt attacaggaa
    34681 gatgtggatg ccttgtttga ggaattgtat aagttctctt acaaaatttt gctggaaact
    34741 aacggagcta tctctctgga aaaagttcct gattatgtaa ttaaaattgt agatgtaaaa
    34801 acccccggca gtggaatgga aaagagtttt cttcctgaaa acctgcaata tctgaatccc
    34861 caggacgaac tgaagtttgt gataactgat aaagttgact atcagtttgc cgttgatttc
    34921 ttaaaaaagt ataaacccca agttaggtgt gttcattttt caccggttac agaactttta
    34981 aaccccaaag aattggcttg ctggatgctg gaagatggac ttgaagcaag attgacattg
    35041 caattgcata aaataatcgg gatggcataa aattaccttg cctaaagcgc aaaaagagat
    35101 agttttttac aaagaggaaa gagaaaaatt gactttatcc tattatgttc cctaaggatg
    35161 agaaaagatg attaaaagtt tttgtattca atgtagacag aggatgcttt tcctgcgcaa
    35221 aaaaatacgg agcatcagca aactcaagct acacaaatta cggagctaca gaaagctccg
    35281 actacataat ggaggttcaa attatgaaaa gtgtttatgg cttactttta ataggtgtca
    35341 ttctggtttt ttttagtcct gctttgctat ccgataccgt taaattgact aacggaaatg
    35401 taatttatgg acatatcacc agagaagaca tagacaaagt tacaattgta gatcaaaatg
    35461 gaattgaaaa catcattcca cgtaacctta tcatctttat tgataaacaa ttagagacaa
    35521 caagagaggt tcagaaagta attattactt taaagagcgg aattgaggtt aaaggaactt
    35581 taatcctgga cgatgctaac agcgtaacta tcaaagatga atcgggaatt gagaagaaaa
    35641 tagcaaaaag caatattcgc gatattgtta ttgatttaga taaaccggac gaaagaccaa
    35701 ttactattaa aagttatgaa gaacccgaaa cggaaataga aactgaaacg gaagcggaaa
    35761 ttgttaccac tcctgcggaa aaaaccaata attttgattt tggactaaaa ttcggctctc
    35821 agaaacccaa ttcaaatgct atttctgata aatacagtaa tggaattgta tatggaggtg
    35881 aactgatttt ttggctgaaa aacaaatacg gaatcggact aaatgttgaa aaattcagtg
    35941 aaaaatcaga atattggttc tataatgagg gtaattatga atataatatt tctattattc
    36001 ctattactct aacagtttat tataagcatt ataataataa aatagtgcct tatattggtt
    36061 taggtattgg acaatataat ctaactgaaa aaattaaata tccctggtat tactattata
    36121 aagaattaga ggtatcagat gaagaatatg gctttcactg tttatttggt ttacaatata
    36181 agattttatt cgttgaagta aaatactcca cgatgtttaa aagtacctgg ggagctgaag
    36241 aagaatatga tgatattggg ggcttgagta ttgcaggcgg gctcaggttt taattaacaa
    36301 agttccgaaa aattactatt tactccggaa taacgatgtc ccatcgttaa tttctaccca
    36361 atttcaaccc aacctcaagg gtgggtggat gatgagaaga ggatgagttg tttacaggaa
    36421 tcaagtaagg atgttttccc caaggggatg attatataaa atacaggtag ttgcttatat
    36481 ttttcaacca ccgctgatca gatgaataac tttgatatcg gcattttctg gaatgggaat
    36541 attctgccag ttttctcttt tgataagttc accgttcatt ttaataacca gcattttgaa
    36601 ggtgtaattc atcattttca aagcatctgc aacaacgagt ccttctttcc agggtaattt
    36661 atttccgttt acaaagaaaa caggtttatc tgtcatttgt tacattcctt ttgtttccgc
    36721 aagtagttcc aaagccaaat ttgcctgcat attggctaca acggcaacac gggttgcaac
    36781 ggggcttatt tccgcattta attcactttt catatcgcct acaagatgca aaaaaccgta
    36841 atgttccgta tgtatgcttt cgtttttacc gtagccagcc aatcctgaag ccacaattaa
    36901 atgtttattc ggataaatag atgtccagca ggaaattagc atttcttttt gttctgcctc
    36961 atccaacgct tctaaaagca ggtctgcagt tccaaaaata gcgttaaaat tggcaggagt
    37021 aatttttacc ggatgcaaaa caagattaat aaaggggtta aaggactgaa tattttccgc
    37081 taatgcctcc actttcaatt tgccaatttg ggctatagta tattgctggc gattaagatt
    37141 ttccagcgta acaatgtcgt aatcggcaat aatcaaagtt ccgattccgg ctctggctaa
    37201 ggaaaaggca atatttgaac ccaaacctcc cgctccggca attcctacag ttgctttttg
    37261 ccaaagagga aaggcggaag gatcgcgctt ggaaaaatac tcttcccggg aaatcaacgc
    37321 tttatctcca acctaatatt gcctttgggg tctatatctt taaggttatt cacaaaagca
    37381 ataacgatat cctgtaataa tttttgcaca aaaggcacaa tcacaatttc cctatccccc
    37441 acccaaagtc tgagttctgc ttgttcagcc aaaacgcaat cactcctttt tgccctgccc
    37501 tgaataatat cttccgccat agtataacag tcctttccac aggcagaaca gcattccggc
    37561 tctgctaaag gcaaaatatc aaagcatttt gcttcaatcg tgcttagtaa aacagccaaa
    37621 tcatccggta aggaaaaaac tggtaaaccc ttaaaactct tcattttaga agaaataacc
    37681 ccggaaattc caatgcaggt gtcatcaatc aattcatcca gttgatccgt attttccgca
    37741 caaacaattt tagacacagg agcattttgc agaccttcaa taattagata atccgcttgc
    37801 aggtaggaaa ccatttcttt taaattcagc ggtcttggaa acaataaggc actgtcatat
    37861 aagcccttag cgaaaacagc tttacttcct gcctgagtat ggagaaaagt atttttacct
    37921 tcagtgtcgg aatgatatgt ttcactatgt atgtctttga tagagcaaac attatatccc
    37981 ttctttgtca gttctcttat caggcaggtt gccaaagttg ttttgccggt ttgatggtag
    38041 ccaataattc ctaatgcctt catatttaat ccgttacaag cagtaattga caaaaagcgc
    38101 taattccttc tccttttcca ctgatgccaa gtccttcttc agttgttgct ttaagggaaa
    38161 tttgctctat ggcacaatgc aaatccaggg caagattagc cctcattgca tttataaagg
    38221 attggagttt tggttcttga cagcagataa cggcatccaa attatttaat ttccagccct
    38281 tttccgtgat taaagtatat gttttgcgca gtaaaatgcg ggagtcaata tccttataag
    38341 ccaaatcgtt atcgggataa aatgtcccga tatcccctaa agccaaagct cctaaaagag
    38401 catcaataat gctgtgaatt aacacatcgg catcggaatg acccgaaagc cccttggaat
    38461 aaggaatttt tacccctccc aaaataagtt ccctatcgga aacaaggcaa tgaacatcgt
    38521 agccaaaacc gcatcggaac atttacttaa tccactgtta tacttttgga tacattttta
    38581 ggaacatcag gatgaacacc atgatcaaga actcccaatt tatccagttt tttcattttc
    38641 ctcacgctca tttttagagc cagcaattgc agaacaatac ttgcggaaaa acaggttaat
    38701 agagtagaac ctgttttggg caacataata taagaccagg cataatagct gttttcactg
    38761 ggtcgtgtag aggcattttt cagcagttgc tcattttctt cagcaatcac aaaagtattg
    38821 gcaccccgta ttttatgggt atttatctga gagatggtga gatttacatc gcgctcatcc
    38881 ggtcctgtaa cataaattaa tggataatta cgatagagat gttcaaagaa atccttatcc
    38941 tgaacaaacc ggttgaaaat attagtccct tgaggtgaaa ggttgaaggg ctgattatga
    39001 gtaaaaatat aatcagccaa tgctttataa acttcctttg tttcgctgtg cggaatgcct
    39061 tcttgttcgc aaagcgactc tatttcatca atgttttcac taaaataacg caagagagaa
    39121 cgcagatgct taacgccaaa aacggtgttt ttccctaaaa ttgtattggg accatgtttg
    39181 aattcggatg cctctcttcc ttcggcatgc gttaaaactg tctctcttat ttttaaagca
    39241 ccttccatag ccacgccact gattttggta gctaaaatat gaatggaggg ttccatataa
    39301 atcttaccag ccataaattc aatttcacca ctcacattat ccagggtctc tttcaaaagg
    39361 gaaggaattt tggcaaggga aacaaagtaa tcatcaagtt cctgttgacg ccataaacgc
    39421 tgttcatgag taagattatt atctatttca ttcaatttca tctgggctgt tttaatggca
    39481 agataataga acagagtaat ttgattcata aagctcttgg tggcaggaac ggcaatttcc
    39541 ggtccgcata aaataggaat tgccacat
//
LOCUS       DAOPFL010000004        39050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_949_length_39050_cov_93.875933, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000004 DAOPFL010000000
VERSION     DAOPFL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 39050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39050
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_949_length_39050_cov_93.875933"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..74)
                     /locus_tag="PLG69_00880"
     CDS             complement(<1..74)
                     /locus_tag="PLG69_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPI42092.1"
                     /translation="MNKIRVRFAPSPTGFLHIGGLRTAL"
     gene            complement(280..861)
                     /locus_tag="PLG69_00885"
     CDS             complement(280..861)
                     /locus_tag="PLG69_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42093.1"
                     /translation="MRKDNTNQKAIDIKQVISLELNQHPELKLIDIYKLVFQAYLGPA
                     HLLADTNESAKKIMTEILLHPEPYKPLKQDIGSQNGFFRLSLDCLVPVLQLKKEKAIS
                     ELYRKSFLLAELMQESVLDFTYNLDITKLWKETIPVIQQLIVFDINEWQQVNAIAESK
                     AIPGHSQQYKTLYNPKYRVLKYQFYDDIEKLLQ"
     gene            complement(848..2311)
                     /locus_tag="PLG69_00890"
     CDS             complement(848..2311)
                     /locus_tag="PLG69_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPI42094.1"
                     /translation="MKILANLYLWLNGKFTEETQSLLVDKGIIIEHLPYPVPEQYKNI
                     FQQNFNGAYAYPGFIDTHTHSFEGGLYCSGVDLSKVKNIADVLELLSAENAKKTKEEI
                     IFAWQLDETLLQEKRFPTVQELDSAVPDKALLLRRIDGHSCMLNSFARKQIKELNTKN
                     EVLKGAENDRAVRHFHSCLTDEEIIEAYQVSAKIGLEGGFTSLHTMIGDAHNSIAHYE
                     LIRDNLDKFPLRFILYPQSFNISAALDAGAKRIGGCILADGSIGSETAAVSEPYLNST
                     SKGVLYQSDEFWQKFISEASKHHLQVAVHCIGDRAIRQINNVYKNISATEELRCELIH
                     CELTDDELIREIAVSKAVPVMQPNFDLLWGGDNGFYAQKLGIKRSQIMNRFATILKAG
                     ITITGGSDWYVTPLNAMQSINAAINHHNPQERLTLAQAIDIYTKNAAWLNFEENITGQ
                     IKTGFQADLSIYSLPLETETSQLRYVIREGEIEYAER"
     gene            complement(2308..2901)
                     /locus_tag="PLG69_00895"
     CDS             complement(2308..2901)
                     /locus_tag="PLG69_00895"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPI42095.1"
                     /translation="MIHELLRHKKIILASVSPRRKELFSLLGISFEAIPADIEEYINS
                     EAPQTQAMQNALRKAQFVKDKVDKDALVVSADTIVVLDNHILGKPADTKEAGNYLRLL
                     SGRSHSVFTGICIYYNEMANINYEQTFVTFAELSETEIESYLATGEPLDKAGAYGIQG
                     FGAQFVTKVEGCYFNVMGFPIRLFYEMLKTILVEEEQ"
     gene            3043..4128
                     /gene="serC"
                     /locus_tag="PLG69_00900"
     CDS             3043..4128
                     /gene="serC"
                     /locus_tag="PLG69_00900"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPI42096.1"
                     /translation="MERVHNFNAGPAVLPEEVLREAQADLVNYKGMGLSVMEMSHRSK
                     EYEAIINEARDAAKRIYGLGDDWDVLFLHGGASLQFVMIPMNFLPEGKIANFIHTGVW
                     SKKAMAEVKKLSKPVHIAASSEDKNFSYIPKTYQLSDNPAYLHITTNNTIYGTEWKID
                     PDVPKDVPLIADMSSNFMSKPIDINKYSFIYAGAQKNIGPAGCAVILVKKDFLATGAT
                     NLPSMLDYQLHSKNGSLYNTPPCFTIYMIGLVLKWIENYGGLARIEENNKAKAKYIYD
                     VIDASDGFYRGTVVPEDRSLMNITFRLPSEELENLFISEAKKNGMIGLKGHRDVGGCR
                     ASCYNALPLNAAYDLAEFMKNFQKQNG"
     gene            4230..4856
                     /locus_tag="PLG69_00905"
     CDS             4230..4856
                     /locus_tag="PLG69_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPI42097.1"
                     /translation="MSILDLIVKGGFLMYVLTVISVISIGIIIEKYRQFRKVRLANNK
                     LQLSLKSQDKLENIRALINMHKSECPMGLMLDKLFNNESDDLDLINQSMEATANMELH
                     RLEKGLGWLSTFAAIAPLIGFLGTVIGMVNVFMKIQAASQSGVDISMLAGGIWVALLT
                     TVGGLIVGIATIIFYNDLVQKLENIVKDMQENAVEYIIKYKKLKQGKL"
     gene            4853..5257
                     /locus_tag="PLG69_00910"
     CDS             4853..5257
                     /locus_tag="PLG69_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPI42098.1"
                     /translation="MKLRVSKQRISTTMLISMTDVIFLLLLFLLIASNFVSQTGLPIK
                     LPGSSTAVRQSYQTLLITYYNDGRLYFMDKPIEIKELETVLQQKYQNPEQVVRIAVEK
                     QVPVQKLINLMDIVRNSGYERIFVATTVLEQK"
     gene            5257..5979
                     /locus_tag="PLG69_00915"
     CDS             5257..5979
                     /locus_tag="PLG69_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42099.1"
                     /translation="MRRFQFSKLFNGYFISVAFHLLLFILFAFYVIQPYVPEKWYTFE
                     WEPLDRTTVIETHTANKLEEITMGNNIPGTEGITKAAGISEKKEINTVNGNPVSIGDS
                     EPAIAMPINVLPKDNAKTTIPSSFPRNRNSNALKEVPGTITGSNFGFSSSLEESGGEA
                     YIISQTKPQINPTEEGEVYLEFKLTPKGTVDMSSVNILSYTSATYAEAVRKVMPSWKF
                     GFKGTYNPKQLYRLRCRFVINE"
     gene            5972..6805
                     /gene="folP"
                     /locus_tag="PLG69_00920"
     CDS             5972..6805
                     /gene="folP"
                     /locus_tag="PLG69_00920"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPI42100.1"
                     /translation="MNKYCFPERIPVLMGILNVTEDSFSDGAQFLDRELAIKHTEVMI
                     AEGAEIIDIGAESTRPGSLPVPAEIQEQRIIPILSSLKEKYPEVTFSVDTQEATVAEK
                     AIELGASIINDISALRTDPALAELLSANPEVKVILMHMQGTPRTMQINPVYQDVLVEI
                     KDFFQERIDFCLAKGILRENIMLDPGIGFGKNLEHNLTILGNLRTFKEFGLPLVVGAS
                     RKSFIDKISPSSPAQRIGGCLAAAFVSAYEEIDILRVHDVLLHRQFFEVLTAIAKAKR
                     D"
     gene            6806..7552
                     /gene="cdaA"
                     /locus_tag="PLG69_00925"
     CDS             6806..7552
                     /gene="cdaA"
                     /locus_tag="PLG69_00925"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPI42101.1"
                     /translation="MEFLIPRFKDIVDILIIAFLIYQALLIVRKSGGYQVLWGLLFVV
                     VLYILAVIFNLKMVSSLLSAIRNYWFIAVVILFQPELRSILARLNLPRELSKVFSKQE
                     KSSLYTPLIDAVSSMSFRKIGALIVLENKRKLHEYIYSGEPIDAIVSMRLILTIFNPK
                     SVLHDGAIIIRDQRIMAAKVVLPLSKKAEYVRKFGTRHLAGIGITEISDAIAIIVSEQ
                     TGQVSVAKAGKIQTNVAFEELLQILTDVTK"
     gene            7539..8183
                     /gene="coaE"
                     /locus_tag="PLG69_00930"
     CDS             7539..8183
                     /gene="coaE"
                     /locus_tag="PLG69_00930"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424716.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPI42102.1"
                     /translation="MSQNKKPLLIGITGNIGSGKSAFCNFLAANGLKVISADAVANQH
                     LEDPAIKEALIKRYSTAILSPSNEDNGKGIINRKILADIVFGSEEETQYLNSLIHPLV
                     LQDFQRIVEQSNEEALCFEVPLLFEANLQDCFDYIVLISASLETRLMRLEKRGEDRTK
                     AQQRMLHQMTDTEKRFLVNLVIENDGDLLSLQKSAVSFIEKIPYLPHKKVRPFV"
     gene            complement(8491..12177)
                     /locus_tag="PLG69_00935"
     CDS             complement(8491..12177)
                     /locus_tag="PLG69_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42103.1"
                     /translation="MKKTLFFVILLLSFIGLTAEQIVVSQYPDDIRLTNSNPNNMELE
                     FTLGSFYREPVTINGDSWYHLFLKKEGLTLEAGMPQVPVLARSLIIPALAKMHLNITN
                     SEYVELTLPVAPSKGNLTRDIDPATIPYTFADFYQSGESYPAEIAYLTEPFILRDYRG
                     ITVRFQPFIYYPATQTLRVYTKLNISVYAQGTDLTNALLNPKTSYSRYFESTYQNMFL
                     NFSAAKYPSLDEEGRILVIKHSMFDATIQPWVDWKRQLGFTVDVVDVTQAGSTAANIK
                     TYIQNQYDLNNGLMFVQLVGDAPQIPTLTYSGGGSDPSYALLAGNDNYYDIFVGRFSA
                     QTVAELETQVARSIYYERDIAPGATWLEKAIGIASAEGGSGQGDMGESDITHMNNIRT
                     DLLNYGYTTVDQIYDPGATAAQVSNSVNQGRGFINYCGHGADTYWVTTNFNNNNANAL
                     TNDYMLPLIVSVACVNGNFVSQTCFAEAWMRSVNETTNAPAGAITFWGSTINQSWAPP
                     MRGQDEVTDLLVGNLKYRVGGLLFNGAHKIIEVYGSQGLADARTWTIFGDASLMVRTK
                     NPQVITATFNPVLFLGMSSFAVQTIPGGRITLTNNGIIYGKGIADATGNCVINLDIIP
                     DLPMDMTLTISAFDYQTYIQTIQVLPSSGPYLVVEETTFSDVNDNIFTTGETVFLDMN
                     LSNIGSETATSVSVTLSTNDSYVTLLNPTLTIGDINSGNVSSTGTFQIQLSNSIPDEH
                     TVNLHIFIATSDGETFENNCSFIAYAPAITWGPLQINDSLGNNNGRIDAGENVTITFN
                     VTNSGHCDAADISTTLIVNGVSHLITPIISTIENLPVSQTGQMIYNVTFSSQIPMGTS
                     VQLTAMTMFGEYLSVHTYTIVVGIVMENFDFGFSNFPWVFEGGNWTISPDSFNGSMAA
                     KSPFINNNSSTSISITYQCPQAGEISFWKKVSSEPSCDFLKFYINGILKFQWSGTDDF
                     WSQVTYPVSPGTNVFKWEYVKNNGSYQGSDCAWLDDIIFPSTGEQIGIPVFVIDMEDI
                     NFGEVIVNTIVSQTVTICNCGTASMLGTIATESPFSLGEPSMPAYYLEYIIPVGESFT
                     FNVNFQPLQNSVYTGTLIINSDDPNALVNTIPLYGTGQPVANDDPVAVLVTSLKGCYP
                     NPFNPTTTISFSIKEKAPVELVIYNILGQKVRTLVNQPLEPGEHSIVWNGTDNKGRSV
                     ASGIYFYRMKAGNYSETKKMVLKK"
     gene            complement(12290..16081)
                     /locus_tag="PLG69_00940"
     CDS             complement(12290..16081)
                     /locus_tag="PLG69_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42104.1"
                     /translation="MKKISFILLLVFTTAILVAASGTIKLQDNPAKVQLLEKSDTGLS
                     IAYAVDELQFREINTKEGIFTELNALNYTTTNVTGLPALPLMRQLISVPLGANVTANI
                     ISSSTKVINLDEQGVLYPLMPRQESVSKSANLEQLPFEVNRDFYNANMWTDNPSITVT
                     EIGMMRGTRIFAVDFVPIKYNPVLKKIEVIYQAEVKVAFSGANFIATNELQAKTYSPA
                     FEGVFAQTLINYNAPKVSFLRYPIGYVIILPDNFIAPMQPFIDWKKQEGYNVIVAPTS
                     ITGTTTTSIKAYMQNLWNSATTENPAPSYLLIVGDVAQVPTNNGNAGSHPTDLTYVRL
                     QGTDYMPEMYFGRFSATSVAEVTNQVNKTLMHEQYTMPSDAYLSEVVMIAGVDSYYST
                     THANGQINYGTNNYFNPAHNITSHTYLYPASGSSASAIVQNASNGMGYINYTAHGDVT
                     EWYNPNFSISNINSLQNTNKYSYVVGNCCLTSKFDNPICFAEAWLRATNKGAIIYIGG
                     TNSTYWDEDYWWGVGYKPPVVGTGSPFVAGRTGAYDALFHDHNEPFADWAGNAGSNVV
                     MGNMAVVQSNSSRINYYWEIYSIMGDPSLVPYLGIPAQNAYTPPAQMFLGFNSMDIQT
                     DPYSYVALSMNNELHGVGLADASGNLTLNYTPFTEPGIAKLVITRSLRKPLIANIEII
                     PNEGPYVTVGPLELNDGNNNIAEAGDNINISLQFNNVGIETASNLTATLTCDSPYILL
                     NNATVQLPDIASGNSINQTNAFQISILPDVPDQHLAGFNITVSDGEDTWVTQRSLTIN
                     APNVVFGNHTLFDSNSDGIYQPGETVTISLSISNTGHMNVDGGSLAIVLNSPYAALDN
                     YNFIIPPLTAGNGIPIVLHLFISQDCPNDEVIPIGFAMNAGIQLLNHSIMIPVGIIGE
                     SFESGDFTAFPWVNNSTNPWTIISGTSNAHFGNYAAKSGAIGNNGSTTLQITMEVSSA
                     GNISFWRKVSSEANYDWLKFYIDNNETGSWSGEIGWSEVSYPVTAGSHIFKWTYSKDV
                     GSVSGSDCAWLDDIRFPGMGVLETAIFYTTTTQIEFTNVLPNTTVSVDFALRNLGNLA
                     MQGIITCPPDFELSQYGNALPANYTYQIEAGQTVLYTLSYIAGNSVSEIQTNMQITTN
                     DPHHPVVIIPVNLYPLANDDNVNIPYVTALHKNYPNPFNPETTIHYSLSEAGPVKIEV
                     YNIKGQLVRNLVNERKTAGNYTVIWNGKDEQGKNVSSGIYFYRMQTKNYSSTKKMMLM
                     K"
     gene            16597..18060
                     /locus_tag="PLG69_00945"
     CDS             16597..18060
                     /locus_tag="PLG69_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPI42105.1"
                     /translation="MPKLITKFDPVIARKNAKRCKERGIILPTIRQQMFPETIPEAIK
                     EKLQPVGLWDINPLNLFRITWKNDIKTGLFGKPNFLEIPSELSGVKARIIGLVGKYFP
                     TGAHKVGAAYGCLVPRLVTGTFDPEIHKAVWPSTGNYCRGGAFDCALLACPAVAILPE
                     EMSKERFDWLREIGAEVFATPGCESNVKEIYDKCAELRSDPSYVILNQFDEFGNSFWH
                     YHITGNAIAEVFAMMQKPGNRLSGFVSATGSAGTIAAGDFLKKHFPLMKINASEAMEC
                     PTLLMNGFGGHRIEGIGDKHIPWIHNVRNTDIVSAIRDEDCMRLLRLFNEPAGLNYLK
                     KNGIKPELAEKLELLGISSICNLLASIKMAKYFEYNEDDVIVTVFTDSAEMYQSRLQE
                     QTALKGEYTELQAALDRESILQAQSYDNLLELSYWDKKRIHNLKYYTWVEQQGKTYQE
                     ILQQWEPEYWIETFENNLEELDKAIEEFNSLGQRIES"
     gene            18091..18615
                     /locus_tag="PLG69_00950"
     CDS             18091..18615
                     /locus_tag="PLG69_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPI42106.1"
                     /translation="MKVTAIILASGKGNRFGLPKAEVKIQGQSFLEKIKETLSQADVD
                     DIFTARYKNTTDMLATLRLAVKELKNKQKNSGFLIFPVDFPFVQAKTVQELIKCHKQN
                     PDAIIRPVYQNKSGHPVLLPFLLNLEQDDKKQGLKYIIQNCGLPIFDVSVEDDGILKN
                     INTKEDLELWMPKN"
     gene            18597..19145
                     /locus_tag="PLG69_00955"
     CDS             18597..19145
                     /locus_tag="PLG69_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPI42107.1"
                     /translation="MDAQELIFGRHSVRDFLPDPIPIDIINQILEAGRCAPSTQNRQP
                     WRYLVFTENAKIRKLALNCGLIGLSNFFIHKAPCLIIACADLKDNIKINGQDYYLVDT
                     AISFHQMLLYARNYGIESCWLAAFSEKKLKGYLKLPAQWKIVAMAPLGYPAEKKGLYS
                     KAVSGFAKSKDRKPLETIVRFM"
     gene            19263..20324
                     /gene="dinB"
                     /locus_tag="PLG69_00960"
     CDS             19263..20324
                     /gene="dinB"
                     /locus_tag="PLG69_00960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPI42108.1"
                     /translation="MKKIIHIDMDAFFAAIEIRENPRLKGKCVIVGGPPNSRGVVSTC
                     SYEARKFGVHSGMSSYQAWNLCPEAIFIHPHFNLYKKVSHQIKEIFYQWTDLVEPLSL
                     DEAYLDVTENKLGEDDAVKIAKLIKAEILEKTGLTCSAGVSYNKFLAKIGSDLNKPDG
                     LTYIPPEKASEVLFSLPIEKFYGIGKVTSAKLKKKGINNGADLYKYELKDLIRLLGKA
                     GHFYYYVVRGIDKREVITEFEPKSLSCETTFYEDIDNLDELLIILQQLAERLSNRLIQ
                     KGIKGNNITLKIKYDNFELITRSSNTPSYINKADELFAYGEQLLIANWDSSRKVRLLG
                     LAIGKLDNTNTDEQLFIPL"
     gene            complement(20307..21227)
                     /locus_tag="PLG69_00965"
     CDS             complement(20307..21227)
                     /locus_tag="PLG69_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPI42109.1"
                     /translation="MSKQNKAYLFAGASILAWSTISTAFKLALNYLTPVGLLFFASLT
                     AFLFLGIVNAVSNPSLFKTKELFKTVGRNLKISFLRGLLNPFIYYLMLFEAYSRLRAQ
                     EAQALNYTWAIVLALFSIWLLKEKFRIIDFVSLLISFFGVWVISTKGQITSLQFDDAL
                     GSFLAVSTSIIWAFYWILNIKDKRPALTKLNYNFFLGFLFIALFALFTHKSLFTPQAI
                     YGYGILGGIYVGIFEMGLTFLLWNKALELTDNTAVISNLIFFTPFLSLLFISSVLKES
                     IHYATFIGLLLIVFSNLLQKGLPAILKRNE"
     gene            complement(21464..22549)
                     /locus_tag="PLG69_00970"
     CDS             complement(21464..22549)
                     /locus_tag="PLG69_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI42110.1"
                     /translation="MKKQIIIFCLMLLVLTACSTARQKLSPQGNVNLKTADVYYSQQD
                     VEQAEIYYLKVLEDNPDHAIALRRLGDISLFKAENFTAREVEFYEDAYHYYAKAISVT
                     EQFPNLTDQDRIDLRDMKKRKERAWTRIFLAAGKEKEAGNTQRAMEIYELAHKLEPER
                     PEPMIQLKNIYLVELKDDVKAEQILQQLLQKDPDKLEYLMEMGTFYYNKGNYAEAVKY
                     FEKARPQIPTNIDNLMNISACYYELKDFEKAMSATKTALEIEPNNVDLLDNARSIAAQ
                     LNDIDQAIFYLKKLLDIRPNEDDFSLLATYLYNKQNWQELIKYAEQWYNWDKSNKIAV
                     EYIIWAAQQTGNKQLETKYTAIKKTMP"
     gene            complement(22761..23534)
                     /locus_tag="PLG69_00975"
     CDS             complement(22761..23534)
                     /locus_tag="PLG69_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPI42111.1"
                     /translation="MQSIILSPLDYIIVLPFIFLAGFIDSIAGGGGLISLPAYWSVGI
                     PPHLALGTNKFSSCCGTLFSTANYFKAKMIDIPVALVSAGMAIIGSWLGASTALRVSS
                     QVLNYLLIILIPAVAIFSLLNRNLGMQSKAEFLAKRLRMLLGGIAGLVIGFYDGFFGP
                     GTGTFLILIYTSLLHYDFVTANGNTKVVNLASNLSALITFAFASNIYYPLAVPGALCG
                     IAGNILGSKLVILRGNKLIRNVFILALLLLFARVIYNLV"
     gene            complement(23538..26786)
                     /locus_tag="PLG69_00980"
     CDS             complement(23538..26786)
                     /locus_tag="PLG69_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42112.1"
                     /translation="MAINKPCFFSLLFILGITTLFAQSLVIEHNLELEPNRYKYPLAN
                     SPIIKNSVSVWADSLLLVEGTDYWVNHQKAELILLTQPEVPVLKVEYILVPEFLTVPL
                     QNWKPQEYSDTLLTSIKKRKNPILAEDARLDIQGTKTFAITFSDESTFDLKQSLFVNI
                     SGELAHNVYINAQLSDSQSKLSPEGDSKELSSLDNVFIRIYGPKYELAMGDLTLKYTG
                     TRYMEYYSKFEGLNAWLRGKHYVQTAFSAGGGKNASVKITIIDGKQGPYYLKANDTQS
                     NFIVVAGSEEIFVDGSKWERGIDYSIDYSEGSVMFKHLISSSNNVLARFQYSDEYYPQ
                     SSYLNSSQLQLAEHLTLTHHFIWQQDDKKNPLLYEFSSADLDSLKSAGDNFVWGEGIY
                     LVEAGTGDYKLIQLSQIEYYEYAPGDSLACYNIVFSYVGSGNGDYEEYSPGKYRYLGS
                     KMGSWLPQKLLVPPEKKGNLDLAVAYSKDNLNAGVEVLGSVNDRNTFSVKDDEDNDAG
                     LLYAYLEVPFSRYSFRLEHEQRSEKTYLFGKYRNPDAEYDFAAIETADSLAQQESNIQ
                     ISRKQTNWNASLLLRYRRIYDLYTLQALRFTSSSSGKGLLPAINLHSTLSAQDYANDD
                     VRDGTLQYHQGDISWAWKILRLRFETLYNLLDTKNYGNSYLKANPGITIGNPSSFLTQ
                     LSYTTDKTKLQENDAWNEISSSNTYALKQMINLGEQRIDLDVTHREVQQPYSTSNPKS
                     NYDLINFRSSNSFLKNSIGFYSNYQLNQTEFYPKIRELQYVGSGLGYYDSTGVSVTDG
                     DYDWVYITSSTGSLSSEINCLLNLYWKPGNVSSMDFIKRLQSDTSINLIEQSFQRNNW
                     KSYFFLPGSVYNEETTIYGRQNIEEVLWLDLIRNKLTANLQISMDRTLDKRYQTPDRT
                     FSFSQVAQFDIKGFNAYNTRLQFSNETNRDTHYLSETDIISASALVQRNLNPQSNIQA
                     ELSCSSESGGKQDGSENYRLYSFAFSPSLKSVFMQKYRLSASLAITYNRLSGSEYFSF
                     LPQKRAGWIPSANLSGVYRINTFCLITLDYRFSDYPKQSSSHELKLEFKAEI"
     gene            complement(26791..27234)
                     /gene="umuD"
                     /locus_tag="PLG69_00985"
     CDS             complement(26791..27234)
                     /gene="umuD"
                     /locus_tag="PLG69_00985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HPI42113.1"
                     /translation="MKLHSDGSIKAIYSATQGKKQYRPLMSSKIPAGFPSPAQDYIEG
                     KLDLNEYLIVHPASTFFVRVDGYSMQGAGIMPDDILIVDRALEATNNRIVIAIVDGEL
                     TVKRLKIKGKKYYLVPENDEFEPILIQEGMDFIIWGVVTYVIHKV"
     gene            complement(27296..28477)
                     /locus_tag="PLG69_00990"
     CDS             complement(27296..28477)
                     /locus_tag="PLG69_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI42114.1"
                     /translation="MKQKIMTFVVLVLLVLLSACGSNKKVQAPVEPVRNPLILAHSAA
                     ARGTEAFNDEFYNDAISAFTEAISLFNEAALTATPADSVAENIEKMNLNIAKSYIELA
                     NESMELSSFDEALDYYSKALNVYKSITPKLITQEELQQNIIGIYNNMAITAKQAGKYE
                     QALTYYDQILKLTPNDADVLYAKFYVLRDNIKDENRAYAVLIDYAELTQDAKAFINVA
                     DSYAEKGNLIEAGKYYQKALAIEPSVDLYRRLANYYSGLKDYNNANLYLQKVADSNPE
                     PSELAQIYALMAKNYEGMNDKKKMVEYYEKSLSIERNPQLALLLASYYNSQKNYAKVI
                     SNANITLSIDPKNADALMLRGVSYYQMKNYSAAKADLERLQNDPKYGTQAKNLLKNIP
                     K"
     gene            28776..29057
                     /locus_tag="PLG69_00995"
     CDS             28776..29057
                     /locus_tag="PLG69_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPI42115.1"
                     /translation="MSRDELVKKIAANAGTSQKVASAALTAVLEGITESLKKGEKVSL
                     VGFGSFSVAHRKARNGINPKTKKPLKIPARNVPVFKAGKNLKEAVKKAK"
     gene            complement(29351..31525)
                     /locus_tag="PLG69_01000"
     CDS             complement(29351..31525)
                     /locus_tag="PLG69_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPI42116.1"
                     /translation="MSYQHFSYSPLTAGKHTVGLAGDFTSWEIIPLEEIGGIYTLSID
                     LPPGVYQYKFIVDGNWIPDKNNPHQVSDNFGGVNSLLIVEEEKEEVTWEDILAQLPNK
                     APEKFYQFFRSDVNNYELRFSWYPKLAETINLLTESWNIEFKRIGQNPLYEVFYCLFK
                     QTGIFSFRIKIQYENKALYFGAEGFSEKEEDISPLKINLKDIPLFAIPDWVSRSIIYQ
                     IFPDRFYNGNKDNDPDFSEWYYADCKEPPPDGKTLSPEKEYYHLVSDWNDISGLKQSP
                     WQKKGIPDFFSFYGGDIAGVRQKLEYLLDLGINVIYFNPLWQAKSNHKYDSADYHSID
                     PHFATTEEMMDFVKIAHQKGIRIILDVAFNHTGETFWAFRDCVEKGPQSPYWNWYDWK
                     KWPLPKPLPPDFNPKEYYQCWWGIKDMPDLNYDLALPHPDENAVRDIRKARPNAPLVD
                     YLISTVRWWLIDIGIDGFRLDVPDEVPFWFWELFRNEVKKAKPDAWIVGEIWHEARQW
                     VSPLYFDSVMNYAFFKTPVIEFFVLNIISKEEFCQKIETGLGLYPFHSLKAMMNLLGS
                     HDTPRIFEICRGNVQRLKLAVIFQMCFIGAPHIYYGDEIAMEGGKDPDNRRPFNWNWE
                     DNPVACEVRAFYKQIISLRKAHPAFWDGHFAFLPAPENAIAFLREGESEKILVFLNLG
                     LDTITISIPDEGEILFGNIDTKKQLQTLSAVILSLPVQPENS"
     gene            complement(31522..34449)
                     /gene="uvrA"
                     /locus_tag="PLG69_01005"
     CDS             complement(31522..34449)
                     /gene="uvrA"
                     /locus_tag="PLG69_01005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPI42117.1"
                     /translation="MKTAIIIKGAQEHNLKNIDLTIPRNNLVVFTGVSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGQMEKPKVDYIEGLSPAISIEQKSASKNPRSTVGTA
                     TEIYDYLRVLYARIGKQYCYNCGRPVGSQTIDQMISHLMQNKPGTKLQILAPIVQNRK
                     GEHKDELEMLRQEGFVRVMINGQLRNLEEEIVLDKKSKHNIDVVVDRIVIKEEIESRL
                     SDSLELALKLADGIVKIDYPEEKQETILSAQNACPHCHIGFEELSPQSFSFNSPIGAC
                     PLCGGLGYKLEFDPDKVITDPELSILEGAVAPWGRLEAKQHSWTLNTVQSLARAYDFS
                     LSTPWYELPEIVKQLILYGSKGKKFKNAWQNENGRGEFMMRFEGIIPQLERRMHETTS
                     EEMRRYYLLYISDKPCPACNGNKLKPASLAVKIAGKNISEITAMSIREAYNFFNKLQL
                     KGNEKIIAEEILKEIKNRLGFLMNVGLHYLTLDRRSPTLSGGEAQRIRLASQIGSQLV
                     GVMYILDEPTIGLHQRDVAKLVAMLLQLRDLGNTVIVVEHDEYTIRTADQIVDFGPRA
                     GIYGGEIIATGTLETILQNPQSLTGAYLSRRLKIPVPEKRIKPDGRMLSIINARHNNL
                     KNLNVDIPLGLFVCVTGVSGSGKSSLINQTLSPFLHNHFYHTAQKVGKMDEIKGIEHI
                     DKIITIDQQPIGRTPRSNPSTYVKLFDPIRELFAQLPASKMRGYTPGRFSFNVKGGRC
                     EACEGAGVKQIEMHFMADIFVTCEVCKGKRYNQETLSIRYKGKNIADVLNMDVQEAIE
                     FFDAIPAIRNKLKTLQEVGLDYLKLGQPSPTLSGGEAQRIKLSRELSKVSTGNTLYVL
                     DEPTTGLHFDDINKLLKVLKKLVAMGNTIIVIEHNMDVIKSADWIIDLGPEGGDEGGK
                     IICSGTPEEVMACPESATGLHLKQQLEKDKEETQNLLLEMNSEDKARKNNLKKKTTKR
                     QAL"
     gene            34885..36105
                     /gene="hydF"
                     /locus_tag="PLG69_01010"
     CDS             34885..36105
                     /gene="hydF"
                     /locus_tag="PLG69_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPI42118.1"
                     /translation="MSSTPRGERLNIAIIGKRNAGKSSLINALVGQDIAIVSAVPGTT
                     TDPVDKRYELLPLGPVTFFDTAGVDDTGELGEKRIKATQKILYRADIAIFVSDGNAFN
                     NAELKMIEKIREMELPMLIVFNKKDIIPVCQENIAFCQNNSLPYISVSSVTKEGIDEC
                     KEKLIQLSPKYLKENRIIAGDLVEPKNSVILVTPIDTSAPKGRLILPQVQTLRDLLDR
                     NCLVTVVRETELKSALEKTKEPPSLIITDSQVIEMVAKIVPEEIPLTTFSILFARYKG
                     ELDILLEGINRLKTLQDGDKILIAEACSHHIQKDDIGRVKIPNWMQKFSQKNLNFEFY
                     AGHDFPENLEEYAVCIHCGGCMITPMEMNRKIMECRRRGVPVTNYGMTISLLKGVLDR
                     TIKPLLKAKAKQAQ"
     gene            36102..36893
                     /locus_tag="PLG69_01015"
     CDS             36102..36893
                     /locus_tag="PLG69_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPI42119.1"
                     /translation="MKISALQFAPRLLDKEYNLKKMKQLLIGLKTDLVVLPELCNSGY
                     VFANKEEVFSVAEEAGKGETYFVLNDIANSINCNIVYGYPELDGNNLFNSCMLIMSDG
                     RFYNYRKTHLFNREKLFFSPGDTGFQVFNILNGVKIGMMICFDWQFPEAARTLALLGA
                     QIICHPANLVLPWCQQAMKTRCLENRVFAVTANRIGTELNAGETVSFTGQSQILNTKG
                     EVLSSLSVSEERTITVEINPEEANDKAVTERNDAFKDRRPEFYKL"
     gene            36890..38893
                     /gene="ligA"
                     /locus_tag="PLG69_01020"
     CDS             36890..38893
                     /gene="ligA"
                     /locus_tag="PLG69_01020"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPI42120.1"
                     /translation="MNKNKLKARIDRLRAEIEHHNRLYYTLAKPEISDYEYDMLVKEL
                     QELEAQYAEGAIEESPTQKVGSDLTPGAKTIPHKVRMASLENAYSLEEVLAFINRTNN
                     DLGYASEYCAELKIDGFSINLYYENGNFIYATTRGDGLEGEDVTENFRLLPDVPFTID
                     YKGAIEIRGEIYIPVQDFLALNEFRRENELKTFANPRNAAAGSIKLKNREEVKSRHLK
                     ALFYALGYYETPLQYKNEIIDKQTRLLDFLDELGFPTSKRTLCKNYQEVEKFCLQMEQ
                     ERYTIPYDIDGIVIKVNSLEDQKKLGWTAKSPKWAIAYKFKPEEKETVLESVSFQVGR
                     TGAVTPVANLKPVFISGSTVSHATLHNADEIKRLDLHYGDTVLVVKSGEIIPKILSVN
                     VSKRPVNAKPVIFPANCPVCNAPLQRDEDGSINYCPNANCPAQIQRKLEHFASREAMD
                     IYGLGSSLIARLIETGQIKWIPDIYHLDYNKIAEMERMGKKSAENLQKAIEASKTKNF
                     DRVLFALGIRHIGSITARTLAQHFKNIDALIMAKEEELLSVPDIGSIVARSIIGFFSQ
                     KSNRELIDSLRKVGLQFTYISPQSSAILAGKTFLITGTLPNYSRKDMETLITEHGGKI
                     LSGVSKQLNYLIVGENPGSKLDKARQLGTVKIISEAELLDMLQ"
BASE COUNT        12386 a         7266 c         7410 g        11988 t
ORIGIN      
        1 agtgcagtcc tcaaacctcc aatatgcaaa aaaccggttg gactgggagc aaaacgcaca
       61 cgaatcttgt tcataatata tttactcctg atttttggat ggcaaattgc gagttgcaat
      121 tgcgaaaaaa gttaactgac ataattttta tattggcaat gctctttgta taaataattg
      181 tttggattcc tgcctgcaca ggaatgacaa aaagattatg caaattataa atatgtaatt
      241 cgttattcca ttcagtaata gcgttatatt cacttaaaat cattgtagta atttctctat
      301 atcatcataa aattgatatt tcagcactcg gtatttgggg ttataaagag ttttgtattg
      361 ctgtgaatgt cctggaatag ctttgctttc agcaatagca ttaacttgct gccattcgtt
      421 aatatcaaat actattagct gttgaataac aggaatagtt tctttccaga gcttagttat
      481 atccaaatta taagtaaaat ccaaaacaga ttcttgcata agttctgcta aaagaaaact
      541 tttacgataa agttcgctga ttgctttttc ttttttcagc tgtaaaacag ggaccagaca
      601 atctaaactt aaacggaaaa aaccattttg ggaaccgatg tcctgtttta atggcttata
      661 aggttcggga tgcaagagta tttctgtcat aattttttta gctgattcat tagtgtcggc
      721 aagcaaatgc gcaggaccca aatatgcttg aaagacaagt ttatagatat caattagttt
      781 tagttccgga tgttgattta gttccagaga aataacctgt ttgatatcta tagctttctg
      841 gttagtatta tctttccgca tattctattt ctccttctct aataacataa cgcaattgag
      901 atgtttcagt ttccagcgga agggaatata tagataaatc agcttgaaag ccggttttaa
      961 tttgtccggt aatgttttct tcaaagttca accaggcagc atttttggtg taaatatcaa
     1021 ttgcctgagc aagagttaaa cgctcctgcg gattatgatg attgattgct gcatttattg
     1081 actgcattgc atttaaagga gttacatacc aatcggaacc cccggtaata gtaattccag
     1141 ctttgagtat tgtggcaaag cgattcatta tttgactacg cttaattcct aatttctggg
     1201 cataaaagcc attatctcca ccccataaaa ggtcaaaatt gggttgcata acgggcactg
     1261 ctttagaaac agcaatttcc ctaattaatt catcatctgt taattcacaa tgaattagct
     1321 cacagcgtaa ttcttcagta gcagagatat ttttataaac attattgatc tgccgaatag
     1381 ctctatctcc gatacagtga actgctacct gcagatgatg tttacttgct tcgctaataa
     1441 atttttgcca gaattcatca ctctgataga gaaccccttt ggaagtgctg tttaaatagg
     1501 gttcagaaac agctgcagtt tcagaaccga tggatccatc tgctaaaata catcctccga
     1561 ttcttttggc tcctgcatcc aaagctgcac taatattgaa ggattgagga tagaggataa
     1621 aacgcagagg gaatttatcc agattatctc tgataagctc atagtgagca atgctgttat
     1681 gagcgtcacc aatcatcgta tgcaaagaag taaagccacc ctccaaacca atttttgcgg
     1741 atacctgata agcttctata atctcctcat ccgttaagca actatgaaaa tggcggactg
     1801 cccgatcatt ttctgctccc tttaaaactt cattttttgt atttaactct ttaatctgct
     1861 tacgggcaaa agaattgagc atacaagaat gaccatcaat tcttcttaag agtaatgctt
     1921 tatcgggaac agccgaatcc agttcttgaa ctgtggggaa acgcttttcc tgcaataagg
     1981 tttcatccaa ttgccaggca aaaataattt cctcttttgt ctttttggca ttttcagcag
     2041 aaagcaattc cagaacatca gcaatgtttt taactttgga taaatctacg cctgagcaat
     2101 ataaaccacc ttcaaagctg tgagtgtgag tatcaataaa accgggataa gcataagcac
     2161 cgttaaaatt ctgttgaaaa atatttttat attgttccgg aacaggatag ggtagatgtt
     2221 caataattat tcccttgtct actaataaag attgggtttc ttctgtaaat ttaccattca
     2281 gccagagata gagattagct aatattttca ctgttcttcc tcaactaaga ttgttttcag
     2341 catttcataa aataagcgga taggaaagcc cataacattg aaatagcatc cttccacttt
     2401 tgttacaaat tgtgctccaa aaccctgaat tccataagcg cctgctttat ctaaaggttc
     2461 tcccgttgcc aaataagatt caatttcggt ttccgacagc tctgcaaaag tcacaaatgt
     2521 ctgctcataa ttaatattag ccatttcatt atagtaaata cagatgccgg taaaaacact
     2581 atgtgagcgt cccgaaagta atcttaaata gtttcctgct tctttagtat ctgcgggctt
     2641 gcctaaaatg tgattatcca aaactactat agtatcggca gaaacaacta aggcatcttt
     2701 atctacttta tccttaacaa attgagcttt tcgtagagca ttttgcattg cctgagtttg
     2761 cggtgcttca ctattaatgt attcttcaat atctgcggga atagcttcaa aggatattcc
     2821 caaaagcgaa aaaagctcct ttctccttgg tgaaacagag gcaaggatta tttttttatg
     2881 gcgtaacaat tcgtgtatca tcatattaaa cctttgtcac agagaataaa aaagcggttt
     2941 taagtcaata tatttctcta tttaccaaaa aatgtcttgc ctaaaagggc ggcattagaa
     3001 aaacttgttt tatattgaaa atatatatcc aggaggatac aaatggaacg cgtacataac
     3061 tttaacgctg gtcctgcagt tctgcctgaa gaagtgctta gagaagcaca ggcagatctt
     3121 gttaattaca aagggatggg actttccgtt atggaaatga gccatcgcag taaagagtat
     3181 gaagccatta tcaatgaagc cagagatgct gcaaaacgga tttatggtct cggtgatgat
     3241 tgggatgtgc tttttctgca tggaggtgca agtttgcaat tcgtgatgat tccaatgaac
     3301 tttctgccgg aaggtaaaat tgcaaacttc attcataccg gtgtgtggag caaaaaagca
     3361 atggcggaag ttaaaaaatt gagtaaacca gtgcatattg ctgccagttc ggaagataaa
     3421 aatttcagtt acattcccaa aacctatcag ctttccgata atcctgccta ccttcacatt
     3481 actacaaaca atacgattta tggcactgaa tggaaaatag accctgatgt tccgaaagat
     3541 gttcctttga ttgcagatat gagctcaaac tttatgagca aaccaattga tatcaacaaa
     3601 tacagcttta tttatgccgg agcacagaaa aatattggtc ctgctggctg tgctgttatt
     3661 ttggtgaaga aggacttttt ggctacagga gctacaaatc tgccatcaat gctggactat
     3721 cagttacatt ctaaaaacgg cagtttgtat aatacccctc cctgtttcac tatctatatg
     3781 attggtttgg ttttaaaatg gattgagaac tatggtggac ttgccagaat tgaagaaaat
     3841 aacaaagcta aggcaaaata catttacgat gttattgatg cctccgatgg tttctataga
     3901 ggaactgtgg ttccggaaga ccgttctttg atgaatatca ctttccgcct tcccagcgaa
     3961 gaactggaaa atctttttat tagtgaagct aaaaagaatg gaatgattgg tctgaaaggt
     4021 catcgtgatg taggtggatg ccgtgcttcc tgttataatg cgcttcctct taatgctgct
     4081 tatgatttgg ctgaatttat gaaaaatttc caaaaacaga atggataatt catacttgaa
     4141 aagaccctga ctattaaaaa ctgctgtagt atctttatag atgctacagc agttaaggtt
     4201 ataagagaaa aaataatacg cgaggttaaa tgtctatctt agatttaatc gtgaaaggtg
     4261 gattcctgat gtatgtgctt acggtaattt ccgtaatcag catcggcatc ataattgaaa
     4321 aatatcgtca attcagaaaa gtacgcctag ctaacaataa acttcagctg tctcttaaat
     4381 cgcaggataa attggaaaat atccgcgctc ttataaatat gcataagagt gaatgtccta
     4441 tgggactgat gctggataaa ctattcaaca acgaaagcga cgacttggat ttaattaacc
     4501 agagtatgga agcaacggca aatatggaat tgcatcgttt ggaaaaagga ctgggttggc
     4561 tttctacttt tgcggcaatt gcacctttga ttggattttt gggcactgtt attgggatgg
     4621 tgaatgtttt tatgaaaatt caggcagcca gtcaaagcgg agttgatatt tctatgctgg
     4681 caggtggaat ctgggttgct ttgttaacta cggtaggggg tttaattgta ggtattgcga
     4741 caatcatttt ttacaacgat ctggtgcaaa aattggaaaa tatcgtgaaa gatatgcaag
     4801 aaaatgcagt ggaatatatc attaaatata agaagttaaa acaaggtaaa ctatgaaact
     4861 gcgtgtttcc aaacagcgaa tttctacaac aatgcttatt tcaatgaccg atgttatctt
     4921 ccttcttctg ctgtttttac tgattgcttc aaacttcgta agccaaaccg gattaccgat
     4981 taagcttcct ggttcttcca ctgctgtccg gcaaagttat caaaccctgt taattacata
     5041 ttataatgac ggacgcctgt attttatgga taagcccata gaaattaaag aactggaaac
     5101 agtccttcag caaaaatacc aaaatcctga gcaagttgtg cgcattgctg ttgaaaaaca
     5161 agttccggtg caaaaactca taaatttaat ggatattgtc cgtaattccg gctatgaaag
     5221 aatctttgtt gccacaactg ttctggagca aaaatagtgc gccgttttca attttctaaa
     5281 cttttcaacg gctatttcat ctctgtagca tttcacctgt tgttgtttat tctttttgcc
     5341 ttctatgtta tccaacctta tgttccggaa aaatggtata cttttgaatg ggaaccctta
     5401 gataggacta ccgtgattga aacgcatacg gctaataaac tggaagaaat aactatgggt
     5461 aacaatattc ccggaactga gggaataact aaagcggcag gaatttctga aaaaaaagaa
     5521 ataaataccg tgaatggaaa tcctgtaagt ataggtgata gtgaacctgc tattgctatg
     5581 ccgataaatg tgttacctaa ggataatgcc aaaacgacaa ttccttccag ctttcctcgt
     5641 aatagaaatt ccaatgcttt gaaagaagta cccggaacca taacaggcag taatttcggc
     5701 ttttcttctt ccctggaaga aagcggggga gaagcatata taatcagcca aacaaaacct
     5761 caaataaatc ctactgaaga gggtgaggtc tatctggaat ttaaacttac tcctaaaggc
     5821 acagtagata tgagcagcgt aaatatactt tcttatacca gcgcaactta tgccgaagca
     5881 gtgagaaaag tgatgccttc ctggaagttt ggttttaagg gaacttataa ccccaaacaa
     5941 ctttatcgtt tgcgttgcag gtttgttatt aatgaataaa tattgctttc cggaacggat
     6001 acctgtttta atgggtattt tgaatgttac tgaggattct ttttcggacg gagcacaatt
     6061 cctggacaga gaattagcta ttaaacatac ggaggtaatg attgctgaag gagcagaaat
     6121 aattgatatt ggagcagaat caactcgtcc tggttcttta cctgttcctg cagagataca
     6181 agaacaaagg ataatcccta ttttaagctc attaaaagaa aaatatcctg aagttacttt
     6241 ttcagttgat actcaggagg caacagttgc tgaaaaggca atagagcttg gcgcttctat
     6301 tatcaatgat atttcggctc ttagaaccga tcctgctttg gcagagcttt tatcagcaaa
     6361 tcctgaggta aaagttattt taatgcatat gcagggaact ccgcgcacaa tgcaaataaa
     6421 tcctgtttat caggatgtgc tggttgaaat aaaggatttc tttcaagagc gtattgattt
     6481 ttgtcttgcc aaagggattt taagagaaaa cataatgctt gatcccggta ttggatttgg
     6541 caaaaacctg gaacataatt taactattct tggcaatctg agaacattta aggaatttgg
     6601 cttaccgctt gtagtaggtg ctagcagaaa gagctttata gacaaaattt caccttcgtc
     6661 gcctgcacaa cgcataggag gatgccttgc agcagctttc gtttctgctt atgaagaaat
     6721 tgacatttta cgtgttcatg atgttctttt gcacaggcag ttttttgagg tcttaactgc
     6781 tattgccaaa gcgaaaaggg actaaatgga atttttaatt cctcgcttta aagatatagt
     6841 agatatcctt attattgcat ttctgattta ccaggcacta ttgattgtgc gtaaatcagg
     6901 tggttatcag gttttatggg gtctgctgtt tgtagttgtt ctctatattt tagccgttat
     6961 cttcaatttg aagatggtta gttcactttt aagtgccatt cgcaattatt ggttcattgc
     7021 cgtcgttatt ttgtttcaac cggaattgcg ttctatttta gctcgcttga atctgccccg
     7081 ggaactaagc aaggtcttta gcaaacagga aaaatcatcg ctttatactc ccctgataga
     7141 tgctgtttct tcaatgagtt tccgtaaaat aggcgctctg attgtgttgg aaaataaaag
     7201 gaaactgcat gaatatattt atagcggtga acccattgat gctattgtgt ctatgcgttt
     7261 gatccttact attttcaatc ctaaaagtgt gttgcatgat ggagctatta ttattcgtga
     7321 ccaacgcatt atggctgcta aagtagtttt gccactatct aaaaaggcag aatatgtgcg
     7381 taaatttggt actaggcatc ttgctggtat tggaataaca gaaataagcg atgctattgc
     7441 tattattgtt tccgaacaaa caggtcaggt ttctgtagcc aaagcgggaa aaatacaaac
     7501 caatgttgct tttgaagaac ttctacaaat tttaaccgat gtcacaaaat aaaaaacctc
     7561 tgcttatagg tataacggga aatataggca gtggcaaaag tgctttttgc aattttctgg
     7621 ctgctaatgg cttgaaagtt atttctgcag atgcagttgc caatcaacat ttggaagacc
     7681 cagcaatcaa agaagcatta ataaaacgct attctacggc tattctatct ccttccaatg
     7741 aagataatgg caaaggtata attaatcgta agattttggc agatatagta tttggcagtg
     7801 aagaggaaac ccaatactta aattctttaa tacatcctct tgtgttacag gattttcaaa
     7861 gaatagtgga acaaagtaat gaggaagctc tctgctttga ggttcctctt ctttttgagg
     7921 caaatttaca ggattgcttt gattatattg tattgataag tgcttctctg gaaacacgac
     7981 ttatgcgttt ggaaaaaaga ggagaagaca gaaccaaagc tcaacaaagg atgttacacc
     8041 agatgacgga tacagaaaag cgttttctgg taaatttagt tattgaaaat gatggtgatt
     8101 tgcttagttt acaaaagtca gcagtatctt ttatagaaaa aatcccttat ctaccacata
     8161 aaaaagtccg cccttttgtg tagcccgagg acgctgttca tcggcatctg tgtaaagaca
     8221 attgtcacga gccatcactg tcaaataaaa aatagtgtat aacataaaat ttattttagc
     8281 agtaacgact cttgacttgc atagtgacgc ctcctgccta tgatgggtaa aagaaaaatg
     8341 acagggatgt cgtttttcca acaaaaaaaa gcggagctac aatgagctcc gcctacactt
     8401 tttttcaaat ttccttagga ttcctgcttg tgcaggaatg acaaagggtt tttcaatcta
     8461 agtatgatac aatctcttga cctcaagacc tcattttttc agcaccattt ttttggtctc
     8521 ggaataattt cctgccttca ttctatagaa atagattccg ctggctacag aacgaccctt
     8581 attatcagtt ccattccaaa caatgctatg ttctcctggt tctaagggtt ggttaaccaa
     8641 agttcggact ttttgtccta aaatattgta aataaccagt tccacaggtg ccttttcctt
     8701 tatgctgaaa ctgattgtag ttgtaggatt aaaaggatta ggatagcatc cttttagaga
     8761 agttaccaaa acagcaacag gatcgtcatt ggcaaccggt tgtcctgttc cgtataaggg
     8821 aatagtattt actaaggcat tggggtcgtc agagtttatt attagggttc cagtgtatac
     8881 tgagttttgt aggggctgga aattcacatt aaaagtaaag gattcaccta ccggaataat
     8941 gtattccaaa taataagcag gcatagaagg ttctcctaaa gagaaaggtg attctgttgc
     9001 aatagttccc agcattgaag cagttccgca attgcatata gtaacagttt gggagacgat
     9061 agtattgact ataacttccc caaaatttat atcttccata tcaattacaa atacaggtat
     9121 tccaatttgt tcgcctgtgc tggggaaaat aatatcatca agccatgcac aatcgctacc
     9181 ttgataggat ccgttgttct taacatattc ccatttgaaa acattagttc caggagaaac
     9241 aggataagta acctgactcc agaaatcatc ggttccgctc cattggaatt ttaaaattcc
     9301 attgatgtag aatttaagga aatcgcaact tggttcggaa gagacctttt tccagaaaga
     9361 tatttctcct gcttgagggc attgataagt aatgcttata gaagtgctgg agttattatt
     9421 aatgaaaggt gatttggcag ccatagaacc attaaaacta tctggagaaa tagtccaatt
     9481 tccaccttca aaaacccagg ggaaattgct gaaaccaaaa tcaaagttct ccattactat
     9541 tcccactaca attgtatagg tatgaacact taagtattca ccaaacattg tcattgctgt
     9601 aagctgaaca gaagttccca ttggaatttg agaactgaaa gttacattat aaatcatctg
     9661 accggtttgg ctaacaggca gattttcaat agtggaaata ataggagtta tcaaatggga
     9721 tactccattt acaatcaaag tagtggaaat atcggcagca tcacagtgtc ccgaattagt
     9781 tacattaaag gtaatagtta cattttcacc ggcatcaatg cggccgttat tattacctaa
     9841 actatcattt atctgtaatg gaccccaggt aatagccgga gcatacgcta taaagctaca
     9901 attattttca aaagtttctc catcactggt agcaataaat atgtgtaaat ttaccgtatg
     9961 ttcatcggga atgctgttgg aaagctgaat ttgaaaagtt cctgtagagc taacatttcc
    10021 actgttaata tctccaattg tgagagtagg gtttaacagg gttacataag aatcgttggt
    10081 ggatagagta acactcacac tggtggcagt ttcactaccg atattagaaa ggttcatatc
    10141 cagaaagacg gtttcccctg tagtaaaaat attatcatta acatcgctga aagtagtttc
    10201 ttctaccact aaatagggtc cgctactggg taaaacctgt atggtttgaa tataggtctg
    10261 ataatcaaat gcggaaatag ttaaagtcat atccattggc agatcgggaa taatatccag
    10321 attgataaca caattaccgg tagcatcggc aattccttta ccatagataa taccgttatt
    10381 agttaaagta attctaccgc cgggaatggt ttggacagca aaactgctca tacctaaaaa
    10441 caggacagga ttgaaagtag cagtaataac ctgagggttt ttggttctca ccattaacga
    10501 tgcatctcca aaaatagtcc aggttctggc atcagcaaga ccttggctac cataaacttc
    10561 aataatttta tgtgccccgt taaatagtaa gccgccaacc cgatatttta aattgccaac
    10621 taagagatcg gttacttcat cctgaccacg cataggagga gcccaacttt gattaattgt
    10681 agaaccccaa aaagttatgg cacctgcagg agcattagta gtttcgttta cagaacgcat
    10741 ccaggcctca gcaaaacaag tttggctaac aaagtttcca ttcacacagg caacagaaac
    10801 aataagaggt aacatataat cattggttag agcattagca ttgttgttgt tgaaatttgt
    10861 agttacccaa tatgtatcag caccatgacc acaataattg ataaaacctc taccctgatt
    10921 tacagaatta cttacttgag cggcggtagc acctgggtca tagatttgat caacggttgt
    10981 ataaccataa ttaagcaggt cggtgcgaat attattcata tgtgtaatat cgctttcacc
    11041 catatctcct tggccgcttc caccctctgc agaagcaatt ccaattgctt tttccagcca
    11101 ggttgctccc ggagctatat ctcgttcata atatatactg cgagcgactt gagtttccag
    11161 ttcagctact gtttgtgcag aaaaacgacc cacaaagata tcataataat tatcattccc
    11221 tgccaaaagt gcataggaag gatcacttcc tcctccagaa taagtaagag tgggaatttg
    11281 aggcgcatca ccaaccagtt gcacaaacat caagccatta ttcaaatcat actggttttg
    11341 aatgtaggtt tttatatttg cggcagtaga ccccgcttgc gtaacatcaa caacatcaac
    11401 ggtaaaacca agttgtcttt tccaatcaac ccagggttga atagtagcat caaacatact
    11461 gtgtttaata actaaaatcc tgccttcttc atccagagaa ggatatttgg cagcactaaa
    11521 atttaggaac atattctgat aagtgctttc aaaataacgg ctataggatg ttttcggatt
    11581 caaaagagcg tttgtgaggt cagttccctg tgcgtaaaca gaaatattca gttttgtata
    11641 gaccctcagg gtttgagtag caggataata tataaagggt tgaaaacgaa cagttattcc
    11701 gcggtagtca cgcaaaataa aaggttcggt taagtaagca atttcagcag gataactttc
    11761 cccgctctga tagaaatcgg caaatgtgta gggaatagta gcaggatcaa tatcacgggt
    11821 tagatttcct ttggaaggag ctaccggcaa agtaagctca acatattcac tattggtaat
    11881 attcagatgc atttttgcca aagcgggaat aattaaactt cttgctaaaa caggaacttg
    11941 cggcattcct gcttccaaag taagtccttc ctttttcaaa aagagatgat accaactatc
    12001 tccatttatc gtaacaggtt cgcgataaaa actgcccaga gtaaactcca gttccatatt
    12061 attgggattg ctgttagtta aacgaatatc atcaggatat tgactaacaa caatttgttc
    12121 agctgttaag ccaataaaac tcaacagcag aattacgaag aataaggtct ttttcataga
    12181 ttccttcctt tattgctgac taaaatataa ggataatcat tcattatttg aactctcttt
    12241 tccgtgaagc aggcatcctt tcaataagaa tgcctgcttc aaacggtatt tatttcatta
    12301 acatcatttt tttcgtagaa gaatagtttt tggtttgcat ccggtaaaaa tagattccac
    12361 tggaaacatt tttaccctgt tcatcttttc cattccagat aacagtataa ttgcctgcgg
    12421 tcttgcgttc atttacaaga ttacgcacta attgaccctt aatgttatag acctcaattt
    12481 taaccggtcc tgcttcgcta agtgagtaat gaatggttgt ttcagggttg aaaggattag
    12541 gatagttctt atgcagtgcg gtcacataag gaatattaac attatcatca ttggcaagag
    12601 gatacaaatt aacgggtatt atcaccaccg gatgatgagg atcattagtc gtaatttgca
    12661 tattggtttg aatttcactc actgaatttc cagcaatata actgagagtg taaagcactg
    12721 tctgcccagc ttcaatctgg taggtgtaat tagcaggtaa agcattgccg tattgactaa
    12781 gttcaaaatc agggggacag gtaataattc cctgcattgc taagttaccc aaattgcgca
    12841 aggcaaaatc aacgctcacg gttgtattgg gaagaacatt ggtaaactca atttgggtag
    12901 ttgtagtata gaatattgca gtttccaaaa ctcccattcc cgggaaacgg atatcatcca
    12961 accaggcaca atcactacct gaaactgatc caacatcttt agaatatgtc cacttgaaaa
    13021 tatggctacc agcggtaacg ggatagctta cttccgacca accaatttca ccgctccagg
    13081 aaccggtttc attattgtca atatagaatt tgagccaatc gtaatttgct tcactactta
    13141 ctttacgcca gaacgaaata tttcctgcag aagaaacttc catagttatc tgtaaagtgg
    13201 tgctgccatt atttcctata gcacccgatt tggcagcata attaccaaaa tgggcattag
    13261 aagtgccact aataatagtc cagggatttg tgctgttatt tacccaaggg aaagcagtaa
    13321 aatcaccgct ttcaaaactt tcaccaataa taccgacagg gatcataata ctatgattca
    13381 atagttgaat tcccgcattc atagcaaatc cgattggaat aacttcgtca ttaggacaat
    13441 cctgactgat aaacaaatga agaacaatag gtataccatt gcctgctgta agcggaggaa
    13501 tgatgaaatt gtaattgtcc aaagcagcat aaggactgtt taaaacaata gctaatgagc
    13561 caccatcaac attcatatga ccggtattgg aaatagataa ggatattgta actgtttcac
    13621 ctggctgata aattccatcg ctattgctat caaacaatgt gtgattacca aaaactacat
    13681 tgggagcgtt gatagttaaa ctgcgttgag ttacccaggt atcttcacca tcgctaacag
    13741 taatattgaa acctgcaaga tgctgatcag gaacatcagg caggatgctt atctggaaag
    13801 catttgtttg attaatactg ttgccggaag caatatcagg caactggact gtagcattat
    13861 ttaacaagat ataagggcta tcgcaagtga gggtagcagt gagattagaa gcggtttcaa
    13921 ttcctacatt attgaattgc agggaaatat ttatattgtc accggcttca gcaatgttgt
    13981 tatttccatc atttaattcc aaaggtccga cagttacata aggtccctca ttgggtataa
    14041 tttctatatt ggcaattaag ggtttacgca aggaacgggt tattaccagt tttgctattc
    14101 cgggttcagt aaaaggagta taattcaaag ttaaattacc actggcatca gctaaaccaa
    14161 cgccatgcaa ttcattgttc attgaaagag ctacatagct gtaaggatcg gtttgaatat
    14221 ccatactgtt gaaacccaaa aacatttgag caggtggagt ataggcattt tgagctggaa
    14281 ttcccaaata gggaactaaa gaaggatcac ccataatgga ataaatttcc cagtaataat
    14341 ttatccggct actattggat tgcacaactg ccatatttcc cattacaaca ttacttccgg
    14401 catttccagc ccaatcagca aagggttcat tatgatcatg aaagagagca tcataagctc
    14461 ctgtacgacc cgctacaaaa ggagagccag ttcctacaac agggggctta tatccaactc
    14521 cccaccagta atcttcatcc caataagtgc tgttagttcc accgatataa attatagcac
    14581 ctttattagt agcacgtaac catgcttcag caaagcatat tgggttgtca aatttactag
    14641 ttaaacagca atttcccact acataactgt atttattagt attttgcaaa gagttgatat
    14701 tactaattga aaagttagga ttataccatt cagttacatc accatgcgca gtgtaattta
    14761 tatagcccat tccattggaa gcattttgca cgattgctga tgccgaactt cccgatgcag
    14821 gatagagata ggtgtggctg gtaatattat gagcaggatt aaaataatta ttagttccgt
    14881 agttaatctg gccattggca tgggtagtgg aatagtaact atcaacacca gcaatcataa
    14941 ctacttcgct taaataggca tcgctgggca ttgtatattg ttcatgcatt aaggttttat
    15001 tcacttgatt ggttacttcg gctacagaag ttgcggaaaa tctaccaaaa tacatttccg
    15061 gcatataatc agttccctgt aaacgaacat aagtaagatc agtgggatgt gaacctgcat
    15121 ttccattatt ggtaggaacc tgagcaacat cacctacaat aagaagataa gaaggagcag
    15181 gattttcggt tgtagctgaa ttccacaaat tttgcatata tgcctttatg ctggttgtag
    15241 ttgttccggt tatagaagta ggcgctacaa ttacattata accttcttgt tttttccagt
    15301 caataaaagg ttgcattgga gcaataaagt tatccggtag aatgattaca tagccaatcg
    15361 gatagcggag aaaagaaacc tttggtgcat tataattgat aagtgtttgt gcaaatacac
    15421 cttcaaaagc gggagaatat gtttttgctt gaagttcatt ggtagcaata aagttcgccc
    15481 cactaaaagc aactttaact tccgcctgat aaattacctc tatctttttg agaacaggat
    15541 tgtattttat aggtacaaaa tcaacagcaa aaattcttgt tccgcgcatc attccgattt
    15601 ccgttacagt aatggaaggg ttatcagtcc acatattggc attatagaaa tctctgttta
    15661 cttcaaaagg taattgctcc aagtttgctg acttggaaac tgattcctga cgcggcatta
    15721 gaggatataa aacaccctgt tcatccaaat taataacctt agtgctactg ctgataatat
    15781 tggcagtaac atttgcccct aagggaacac taatcagctg tcgcattaaa ggtaacgctg
    15841 gtaagccagt aacattagta gtagtgtaat ttaaggcatt tagttcagta aaaatacctt
    15901 ccttggtatt aatttctcta aactgtaatt catctacagc ataagcgata gaaagccccg
    15961 tatcgctttt ttccagcaat tgcacttttg ccggattatc ttgtaatttt atggttccac
    16021 tggcagctac taagatagct gttgtgaaaa ccagtaagag tatgaaagaa atcttcttca
    16081 ttacttctcc ctttatatta ttcttatctt tatatatttt actgcctcta catataatat
    16141 aagcaagaat gaagccgaaa acagtaaaat attgtaattt tagaggacga aaccccaaaa
    16201 taaatcttat ccgagttgtt gattattcgc taacatttac ataaataaat gagttaactt
    16261 atttagctta atagcattgc ttaagcatta cggactcatt gctaatgcca ttagtaatgg
    16321 gtaagcaatg ggtaagcaat gctattaagg aagaactgaa aattgttttg taacatcata
    16381 agaatatgaa tgttagcatt gatgcaaagg taaaaggaaa ctataataac agttaaattt
    16441 gtattggtga aaaagaaaat cggtagtcca caattttcat ctgcattaaa gttttttagc
    16501 tgggagaagt tatttggttt cggcttaaat tcctttacaa aaaacccttc ttttattttt
    16561 ggttctttct attgattgtt aaagaaggag atataaatgc ccaaattgat tacgaaattt
    16621 gaccccgtaa ttgccaggaa aaatgcaaag cgttgtaaag aaagaggaat aattcttcct
    16681 acaattcgtc aacagatgtt tccggaaacg attccagaag cgataaaaga aaaacttcaa
    16741 cctgttggac tttgggatat caatccctta aatctttttc ggattacttg gaaaaatgac
    16801 ataaaaacag gactcttcgg caagcccaat tttttggaaa taccttcaga actttccgga
    16861 gttaaagcca gaattattgg tttggtagga aaatattttc ctacaggagc tcataaagtt
    16921 ggggctgctt acggatgttt ggttccgcgt ttggtaacgg gaacttttga tcccgaaatt
    16981 cataaagctg tttggccttc aacaggaaat tattgtagag gtggtgcttt tgattgtgct
    17041 cttttagctt gcccggcagt tgctatttta cctgaagaaa tgagtaaaga acgttttgat
    17101 tggttgcggg aaattggagc ggaggttttt gccactcccg gatgtgaatc caatgtaaaa
    17161 gagatctatg ataaatgtgc agaattgcgt tcagacccaa gttatgttat tttaaaccaa
    17221 tttgatgaat ttggcaattc tttttggcac tatcatatta ccggtaatgc tattgctgag
    17281 gtctttgcaa tgatgcaaaa accgggcaat cgtttaagcg gatttgttag cgctacaggc
    17341 agtgcaggga caattgcagc gggagatttt ctgaaaaaac attttcccct aatgaaaata
    17401 aacgcttcgg aagctatgga atgtcccact ttattaatga atggttttgg cggtcatcgg
    17461 attgaaggaa tcggcgataa gcatattccc tggattcata atgtccggaa tacggatatt
    17521 gtaagcgcta tcagagatga ggattgtatg cggcttttgc gtttattcaa tgaaccggcg
    17581 ggattaaact atttaaagaa aaatggaata aaaccggaat tggcagaaaa actggaactt
    17641 ttaggtatat catccatctg caatctgctg gcttctatca aaatggcaaa gtattttgaa
    17701 tacaatgaag atgatgttat tgtcacggtt tttacggaca gtgccgaaat gtatcaatcc
    17761 cgtttacagg aacaaacagc tttaaaaggc gaatatactg aattgcaagc agctttagac
    17821 agagaatcaa ttcttcaggc acaaagttat gataatttgc tggaattaag ctattgggat
    17881 aaaaagcgga ttcataatct aaaatactat acctgggtag agcaacaagg taaaacatat
    17941 caggaaatcc ttcagcaatg ggaaccggaa tattggattg agacatttga aaataatctg
    18001 gaggaattgg ataaagcaat tgaggaattc aatagcttag gacagaggat agaaagctga
    18061 gggcaaagag attagggctg agagaatttt atgaaggtga cagctatcat tcttgcttcg
    18121 ggtaaaggga accgatttgg tttgcctaaa gccgaagtga aaattcaagg tcaaagcttt
    18181 ctggaaaaga ttaaggagac cttatcccag gcagatgtag atgatatttt tactgcaaga
    18241 tataaaaata ctaccgatat gctcgccact ttacgcttgg ctgttaagga attgaaaaat
    18301 aaacagaaaa atagcggttt ccttattttt ccggtagatt ttccttttgt gcaggcaaaa
    18361 acggttcagg aacttattaa atgccataaa caaaatccgg atgctattat tcgccccgtt
    18421 tatcaaaata aaagcggaca ccctgttttg ctaccttttt tgttaaactt ggaacaggac
    18481 gataaaaaac agggtttgaa atatattatt cagaactgcg gtttaccgat atttgatgta
    18541 tcggttgagg atgatggtat cttaaaaaac ataaatacaa aagaggattt ggagttatgg
    18601 atgcccaaga attgattttc ggaagacaca gtgtacggga ttttttaccc gaccctattc
    18661 ctatagatat tataaaccaa attttagaag cgggaagatg cgctccttca actcaaaatc
    18721 gtcagccctg gcgttatctg gtttttacgg aaaatgctaa aattcggaaa ttggcattaa
    18781 actgcggatt gatagggctt tccaattttt tcattcataa agcgccctgt ttaattattg
    18841 cctgtgccga tttgaaggat aatataaaaa taaacggaca ggattattat ttggtggata
    18901 cggctatttc ctttcatcaa atgctattat atgcccgcaa ctatggcatt gaaagttgtt
    18961 ggctggctgc tttttccgaa aagaaactaa agggttattt aaaacttccc gctcagtgga
    19021 aaattgtagc tatggctcct ttaggatatc ctgctgagaa aaaaggatta tattccaaag
    19081 cagttagcgg ttttgccaaa agtaaagata gaaagccact ggaaacaatt gtccgtttta
    19141 tgtaaaaacc cttgtattca ccgaatttag attcggcaaa agacgaagta taaattgctt
    19201 ttatctaatt ccgttacttg taacaaaagg gaatttgcca tcccattctt tctcaaagag
    19261 ttatgaagaa gattattcat attgatatgg atgccttttt tgcggcaata gaaattaggg
    19321 aaaatccccg tctgaaaggc aaatgtgtaa tcgttggagg acctccaaat agcagaggtg
    19381 ttgtttccac ctgttcttat gaagcacgca aattcggagt tcatagtgga atgagcagtt
    19441 accaggcatg gaatttgtgt cctgaggcaa tttttattca tccccacttt aatttatata
    19501 aaaaggtctc ccatcagata aaggaaattt tttatcaatg gactgatttg gtagaaccgc
    19561 tttctttgga tgaggcatat ttggatgtta ccgaaaacaa attgggcgaa gatgatgccg
    19621 ttaaaatagc caaattaatt aaagcagaga ttttagaaaa aacgggacta acctgttctg
    19681 ccggagtttc ctataataaa ttcctcgcta aaatcggcag tgatttgaat aaaccggatg
    19741 gtttaactta tattcctccg gaaaaggcaa gcgaagttct tttttctcta cccattgaaa
    19801 aattttacgg catcggcaaa gttacttctg ctaagctaaa gaaaaaggga ataaataatg
    19861 gagctgacct ctataaatac gaattgaagg acttgataag attattaggg aaagctggtc
    19921 atttctatta ttatgttgtt cggggtattg ataaacgcga agtgataacc gaatttgaac
    19981 cgaaatcttt aagctgtgaa acaacttttt atgaggatat tgataatttg gatgaacttt
    20041 taattatttt acaacagctt gcagaacgct tgtccaaccg tctgatacaa aaaggcatta
    20101 agggaaataa cataacccta aaaataaaat acgataactt tgaactgatt acccgcagtt
    20161 ccaatacacc ttcttatatc aataaagctg atgaattatt tgcttacggc gaacagctct
    20221 taattgccaa ttgggatagt tcccggaaag tccgtctcct tggtttggct attggcaaac
    20281 tggacaatac taatacggat gaacaattat tcattccgct ttaatatagc cggtaaacct
    20341 ttctgtaaca ggttgctgaa gacaattaac aaaagtccga taaaagtagc atagtggata
    20401 ctttctttta atacggaact gataaaaagt agcgataaaa aaggagtaaa gaaaatcaga
    20461 ttagaaataa cagccgtatt atcggtcaat tcaagggctt tattccaaag cagaaaagtt
    20521 aaacccattt caaaaatgcc tacataaata cctcccagaa tgccgtaacc gtaaattgct
    20581 tggggcgtaa aaagagattt atgcgtaaaa agagcaaaaa gagctataaa caaaaagccg
    20641 agaaaaaaat tgtaattgag cttcgtaaga gcaggtcttt tatccttaat atttaaaatc
    20701 cagtaaaatg cccaaataat agaagttgaa acagctaaaa atgaacctaa ggcatcatca
    20761 aactgtaagg aagtaatctg cccttttgta gaaataaccc aaaccccgaa aaagctgata
    20821 agtaaagaaa caaaatcaat aatccgaaat ttttctttca atagccaaat actgaataaa
    20881 gctaatacaa ttgcccaagt gtaatttaac gcttgcgctt cttgagctcg caaacgggaa
    20941 tatgcctcaa aaagcattaa ataatagata aaaggattaa gtaatccccg cagaaaagaa
    21001 attttcagat tcctgcctac tgttttaaac aactccttcg tcttaaaaag agaagggtta
    21061 ctaactgcgt taacaattcc cagaaaaaga aaagccgtta aagaagcaaa aaataacaat
    21121 cccacagggg ttaaataatt taaagccaat ttaaatgccg tgctgattgt tgaccaggca
    21181 agaatagaag ccccggcaaa caaatatgcc ttattttgtt tggacattaa ataaattcct
    21241 ttggctcata ttttgtccga agtcatttgc tatattagtt aggagtcatt agcgccttaa
    21301 taattgtaac ccctgaaata aattatacgg cgcttatgac tcgtgacagc acaaaaatct
    21361 ttcacgcaga ttatatagat atcgctgatc tatatcttaa aaacttctta cggaggaacg
    21421 gcgtcctccg accattccta cattttcaac caacccaaaa caattaaggc atagtttttt
    21481 tgatggcagt atatttggtt tccaattgtt tgtttccggt ttgttgagca gcccagataa
    21541 tgtattcaac ggctatttta ttggatttat cccaattata ccactgttcg gcatatttaa
    21601 tcagttcctg ccaattttgt ttattgtaaa gataggtggc aagcaaactg aaatcatcct
    21661 cattcggacg gatatccaat aactttttaa gatagaatat tgcctgatca atatcattaa
    21721 gttgagcagc aatagaacga gcattatcta aaagatcaac attatttggt tctatttcca
    21781 aagcagtttt agtggcggac atagcttttt caaaatcttt cagttcatag tagcaggcag
    21841 agatattcat caaattatca atatttgtag gaatttgagg tcttgctttt tcaaagtatt
    21901 ttacggcttc agcatagtta cccttattgt aatagaaggt tcccatttcc attaaatatt
    21961 ccagtttatc aggatctttc tgtaacaatt gctgaaggat ttgttcggct ttaacatcat
    22021 ctttaagctc aaccagatag atatttttaa gctggatcat tggttcagga cgttccggtt
    22081 ccagtttatg tgccagctca taaatttcca tagcgcgttg tgtatttcct gcttcttttt
    22141 ccttgcctgc agccaagaaa atcctggtcc aagctctttc tttccttttt ttcatatctc
    22201 tcaggtcaat acgatcctga tccgtaagat tgggaaattg ttcagtaacg ctaattgctt
    22261 tggcataata atggtaggca tcttcgtaaa attcaacttc ccgggctgta aaattttccg
    22321 ctttaaacaa actgatatca cccaagcgtc ttaaggcaat agcatgatcc ggattatctt
    22381 caaggacttt caaatagtat atttctgctt gttccacatc ttgttgggaa taatatacat
    22441 cggcagtttt cagattaaca tttccctggg gactaagttt ttgacgagcg gtagaacagg
    22501 cagttagcac taaaagcatt aggcagaaga tgattatttg ctttttcatc cacttttctc
    22561 ctcggattta tttctttttg cacaaaaaaa ttgaatgtgc caaaaagtca agataattgt
    22621 ttccggaagg ttgtagtcct cttcaaccaa attaagtcac gagtccttat tgtcaaataa
    22681 ctttgtggga tatgtaaaat tcttgaaggc aatgatgact cctgacttat aaatgaacaa
    22741 cagggacatc gttcattttg ttacacaaga ttataaatta ctcgggcaaa aagtagcagt
    22801 aaagcgagaa taaaaacatt tctgattagc ttattgcctc ttaaaataac cagtttagaa
    22861 cccagaatat ttcctgctat tccacataag gcacccggaa cagcaagcgg gtaataaata
    22921 ttggaagcaa aggcaaatgt aatcaatgca gatagattgg aagccaaatt tacaactttg
    22981 gtattaccat tagcggttac aaaatcatag tgtaaaaggg aagtataaat taaaatcaga
    23041 aaagttcctg ttccaggacc gaagaatcca tcataaaagc caattactag tcccgctatt
    23101 cctcccaaca gcatacgcaa tctttttgcc agaaactctg ctttgctttg catccccaga
    23161 tttctgttca acaaagaaaa aatagctact gcaggaatca ggataatcaa taaataattg
    23221 agcacttgtg aggaaaccct taatgctgtt gaagcaccaa gccaagagcc aattatagcc
    23281 attccggcac ttactaaggc aacaggaata tcaatcattt tagctttgaa ataattggca
    23341 gtggaaaaaa gagttccgca acaggaagaa aatttattag tgcctaaagc caaatgaggt
    23401 ggtattccga cactccaata agcaggtaaa gaaattaaac ctccacctcc agctatggaa
    23461 tcaataaaac cagcaagaaa aataaaaggc aggacgataa tataatccag gggacttaaa
    23521 ataatacttt gcataactta tatttccgcc ttaaattcca gttttaattc atgcgaactt
    23581 gattgttttg gataatccga aaaacgataa tccagggtaa taaggcaaaa agtattgatt
    23641 ctataaacac cgcttaaatt tgcagaggga atccagcccg ctcttttttg gggtagaaag
    23701 gaaaaatact cgcttcccga aaggcgatta taagttattg ccagagaagc agaaaggcgg
    23761 tatttttgca taaatacact tttcagggaa ggagaaaagg caaaagaata cagacgataa
    23821 ttttcactgc catcttgctt tcctccggat tccgaactac agctcagttc tgcctgaata
    23881 ttgctttgcg gattcaaatt acgctgaact aaagcactgg cacttataat atcagtttcg
    23941 cttaaataat gtgtatcgcg gttggtctca ttgctgaatt gtagacgggt attataagcg
    24001 ttaaaccctt taatatcaaa ttgagcgact tgggagaaac tgaaagttct atccggtgtt
    24061 tgatatcttt tatccaaagt tcggtccatt gaaatctgaa gattggcagt aagtttatta
    24121 cgaatcaaat ccagccacag aacttcttct atattttgtc tgccgtaaat tgttgtttct
    24181 tcattgtata ctgatccggg taaaaagaaa tagcttttcc aattattgcg ttgaaaactt
    24241 tgttcaatca aatttatgga ggtatcgctc tgcaaacgct ttataaaatc catggaagag
    24301 acatttcccg gtttccagta aaggtttagc agacaattta tttctgagga taaacttccc
    24361 gtgctgcttg taatataaac ccaatcgtaa tcaccatccg taacacttac cccggtgctg
    24421 tcataataac ccaaaccgga acccacatat tgcaattcac ggattttggg ataaaattcg
    24481 gtttgattga gttgatagtt actgtaaaag ccgatagaat ttttcagaaa gctattgctg
    24541 gaacggaaat tgatcaggtc gtaattggat ttgggattgg aagtggaata cggttgttgg
    24601 acttcacggt gtgttacatc caggtcaata cgctgttcac caaggttaat catttgtttt
    24661 aaggcgtagg tattagagct ggaaatttcg ttccaggcat cattttcctg gagtttggtt
    24721 ttatctgttg tataggaaag ctgggtaaga aaggatgagg gatttccgat agtgattccg
    24781 gggtttgctt tcagatagct gttcccataa ttttttgtat ccaaaaggtt atatagtgtc
    24841 tcaaaacgca agcgcaatat tttccaagcc caggaaatat ctccctgatg atattgcaaa
    24901 gttccatccc taacatcgtc attagcataa tcctgtgccg aaagagtgct gtgtaaattt
    24961 atggcaggca gcaaaccttt tcctgaagag gaagaagtga aacgcaatgc ttgtaaagtg
    25021 tataaatcat aaatcctacg gtagcgcaaa aggagagatg cattccaatt tgtttgctta
    25081 cgacttattt gaatattgct ttcctgctgt gccaaagaat cggctgtttc tatagctgcg
    25141 aaatcatatt ctgcatcggg attgcgatat ttaccaaaga gataggtttt ttcactgcgt
    25201 tgttcgtgtt ccaacctgaa agaataacga gaaaaaggaa cttccaaata agcataaagc
    25261 aaaccggcat cattatcctc atcatccttt accgaaaaag tattacgatc atttacactg
    25321 cctaaaactt caaccccagc attcaaatta tctttagaat aagcaacagc caaatccaga
    25381 tttccctttt tttcaggggg caccaaaagt ttttgaggca gccaagaacc catttttgaa
    25441 cctaaataac gatatttccc cggagaatat tcttcataat caccgtttcc actgccaaca
    25501 tagctgaaaa caatattata acaggcaaga gaatcacccg gagcatattc atagtattca
    25561 atttgggaca actgaattag tttataatca cctgttcctg cttcaaccaa ataaatacct
    25621 tcaccccaaa caaaattatc ccctgcagat tttaaggaat ccaaatcggc agaactgaat
    25681 tcataaagca agggattttt cttatcatct tgttgccaga tgaaatgatg tgtcagcgtt
    25741 aaatgttctg caagctgtaa ctgagaagaa ttgagataac tgctttgagg ataatattca
    25801 tcagaatatt gaaatcgtgc caatacatta ttggaagagc taataagatg tttaaacatt
    25861 acactgcctt cgctgtaatc aatactgtaa tcaattcccc tttcccattt actgccatcc
    25921 acaaaaatct cttcactacc ggcaacgaca ataaaattgg attgggtatc attagctttt
    25981 aaataataag gaccctgttt tccgtctatg atcgttattt ttacggaggc atttttacct
    26041 cctcctgcag agaaagccgt ttggacataa tgtttacccc tcagccaagc atttagccct
    26101 tcaaatttgc tgtaatattc catatagcgt gtcccggtgt attttagcgt caaatcaccc
    26161 attgcaagtt catatttagg accataaata cgaatgaaaa cattatccag ggaactcaat
    26221 tccttggaat ccccttcagg agaaagtttt gattggctat cggaaagctg ggcattgatg
    26281 taaacattgt gggcaagctc accggaaatg tttacaaaca gagattgctt caaatcaaaa
    26341 gtagattcat cggaaaaagt gatggcaaat gtttttgtgc cttgaatatc caaacgggca
    26401 tcttctgcca gaataggatt ttttcgcttt ttaatagaag ttagcaaggt atcactatat
    26461 tcctgaggtt tccaattttg taacggaaca gttaaaaatt ccggcacaag aatatattcc
    26521 acttttagaa caggaacttc gggttgtgta agcaaaatca attctgcctt ctgatgattt
    26581 acccaataat ctgttccttc cactaatagc aatgaatcag cccaaacgga aacgctgttc
    26641 ttaattatag gtgaatttgc cagaggatat ttatagcgat taggttctaa ttcaagatta
    26701 tgttctataa ccagggactg agcaaacagt gtagtaattc caagaataaa gagcaaacta
    26761 aagaaacagg gcttgtttat tgccacctga ttaaacttta tggataacgt aggttacaac
    26821 accccaaata atgaagtcca ttccttcctg aatgagaatt ggttcaaatt catcattctc
    26881 cggcactaaa taatattttt tccctttaat tttcagtcgt tttactgtta attcaccatc
    26941 cacaatagca attactatgc ggttattagt tgcctccaat gctctatcta caatcagtat
    27001 atcatcgggc ataattcctg caccctgcat tgaatagcca tcaactctta caaagaaagt
    27061 tgaggcagga tgaactatca aatattcatt caaatccagc ttgccttcaa tataatcctg
    27121 tgccggagaa ggaaaaccgg caggaatttt ggaactcatc aagggacgat attgtttttt
    27181 cccttgagta gcagaataaa tagcttttat tgagccgtca ctatgtaatt tcattgtttt
    27241 ttcagaatgc aggacgagtt gccaattcaa atcccgtcct gcatttaaga tttagttatt
    27301 taggtatatt cttcagcaaa ttcttggctt gagttccata tttaggatcg ttttgcaacc
    27361 gttctaaatc agctttagca gcagaataat tcttcatctg ataataggaa acaccacgca
    27421 acattaaagc atcagcattt ttggggtcta tgctaagggt gatattggcg ttgctgatta
    27481 ctttggcata attcttttga gaattgtaat aacttgctaa aagcaatgcc agttgaggat
    27541 tgcgttctat acttaaggac ttttcgtaat attccaccat ctttttctta tcattcattc
    27601 cctcataatt tttagccatc agagcgtata tttgtgccag ttcagagggt tcgggattgg
    27661 agtccgctac cttttgcaaa taaaggttcg cattattata gtccttcaaa ccgctatagt
    27721 aatttgccaa acgacgataa agatcaacgc tgggttcaat agctaatgct ttctgataat
    27781 attttccggc ttcaatcaga ttgccttttt ccgcatagct atctgctaca ttgataaatg
    27841 ccttggcatc ctgagtgagc tctgcatagt caattaaaac tgcataggca cgattttcat
    27901 ccttaatatt atcccgtaaa acataaaact tggcataaag aacatcagca tcgttgggag
    27961 tgagtttcaa tatctgatca taataagtta gcgcctgttc atatttacct gcttgtttag
    28021 ctgtaatagc catattgtta tagataccaa ttatgttctg ttgtagttct tcctgggtta
    28081 tcagtttggg agtgattgac ttataaacat tcagggcttt gctatagtaa tccaatgctt
    28141 catcaaaact gcttaattcc atactttcat tggcaagttc tatataggac ttggcaatgt
    28201 tcaaattcat cttttcaatg ttctcggcaa ctgaatcggc aggagtagca gttagcgcag
    28261 cttcattaaa caaggaaatt gcttctgtaa aagcagaaat agcatcatta taaaattcat
    28321 cgttgaatgc ctcagttcct cttgcagctg ctgaatgagc taaaattaag ggatttcgga
    28381 ccggctcaac gggtgcttga acttttttgt tgctgccaca ggcactaagc aacaccaaaa
    28441 gaacaaggac tacgaaggtc attattttct gtttcattgg taaaacctcc agattttttt
    28501 attaactatg ccctcattgg tctgattttt gtcaacttaa aaaagtataa agcccaaaac
    28561 aaaagaaaaa ttacaaataa ttttttgccc ttgcaataat gcgctaactt actatataat
    28621 aaagtattac gcatattaag aactctgctg aaacctctaa tttcgtaata aaagcgaaaa
    28681 ataagcattt ttttattgac agaattttcg gaatgcactt tgttaacaat tgaaagatgt
    28741 cctactcttt acactaaacc aaataaggag gtaaaatgag cagagacgaa ttggtaaaaa
    28801 agatcgcagc taatgctggt acttctcaaa aagtagccag tgcagccctc acagctgttc
    28861 ttgagggcat aacggaatcc ttgaaaaagg gtgaaaaagt ttctttagtc ggttttggtt
    28921 cttttagcgt tgcacatcgt aaagcgcgca atggtatcaa tcccaaaacc aaaaaacctt
    28981 tgaagatccc ggcacgcaat gttccggttt tcaaagctgg taaaaacctt aaagaggcag
    29041 ttaaaaaagc caaatagttc aattccttta caaacagggc agaagacatt tcttgttttc
    29101 tgcccttttt ttagtcagga gttattaacg cttttaagtt aggagtcatt aagcgttttt
    29161 ataacttttt ttagctggga aatattttta ttggcgttta tgactcgtga gggcaaaaaa
    29221 ttttatacgc agattacgcg gattttatta tgcactaaga acgcagagaa caccgaaaaa
    29281 aatgaagaac tataaattat aacttcctat taatctgttt tattcattta aatccgtgtt
    29341 ttttaagctg tcacgagttc tccggctgca caggtaaaga aagaattaca gcggaaagag
    29401 tttgcaactg ttttttagta tctatatttc cgaagagaat ttcaccctcg tcaggaatac
    29461 ttatagtaat agtatcaaga cccaaattca agaataccaa tattttttca ctttctccct
    29521 cgcgtaaaaa agcaatagca ttttcaggag caggcaagaa agcaaaatga ccatcccaaa
    29581 aagcaggatg agctttcctt aaagaaatta tctgtttata aaaagctctt acttcgcagg
    29641 caacaggatt atcttcccaa ttccagttaa aaggtcttct gttatcagga tcttttccac
    29701 cttccatagc aatttcatct ccgtaataaa tatggggagc tccgataaaa cacatctgaa
    29761 agataacagc caatttaagg cgttgcacat ttccccggca aatttcaaaa atacgaggtg
    29821 tgtcatgact gccaagcaga ttcatcattg ctttcagtga atgaaaagga taaagaccaa
    29881 gtccggtttc tattttttgg caaaattcct ctttgctgat gatatttaac acgaagaact
    29941 caattacggg agttttaaaa aaggcatagt tcattacaga atcaaaatat aaaggagaaa
    30001 cccattgtct tgcttcatgc catatttctc caacaatcca ggcatccggt tttgcctttt
    30061 ttacttcatt ccggaaaagt tcccaaaacc aaaagggaac ttcatccggc acatccagtc
    30121 ggaaaccatc aattccaata tcaattagcc accagcgaac ggtagaaatt aaataatcaa
    30181 ccaaaggcgc attaggacga gcttttctta tatcacgcac agcattttcg tcgggatggg
    30241 gaagagcaag gtcataattc aaatcgggca tatcttttat tccccaccaa cattgataat
    30301 actctttcgg attaaaatcc ggaggaaggg gttttggcag gggccatttt ttccagtcat
    30361 accaattcca gtagggtgat tgaggtcctt tttccacaca atcccgaaaa gcccaaaagg
    30421 tctctccggt atgattgaaa gccacatcca gaattattct tattcctttt tggtgagcta
    30481 tttttacaaa atccatcatt tcttccgtag tagcaaaatg aggatctata ctatggtaat
    30541 ccgcgctgtc atatttatga ttggattttg cctgccacaa aggattgaaa taaataacat
    30601 taatgcccaa atcaagtaga tattcaagct tttgccgcac tcctgcaata tctcctccgt
    30661 aaaaagaaaa aaaatccggt atcccttttt tttgccaggg actttgtttt aatccgctaa
    30721 tatcgttcca gtcacttact aaatgataat attctttttc cggagaaagg gtttttccat
    30781 caggaggtgg ttctttacaa tcggcataat accattcact gaaatcggga tcgttatctt
    30841 tattgccgtt atagaaacgg tcgggaaaaa tctgatagat aatactgcgg ctaacccaat
    30901 ccggaattgc aaacagagga atatctttta ggtttatttt taggggagag atatcttctt
    30961 ccttttcgga aaagccctct gcaccaaaat acagagcttt attttcatat tgaattttta
    31021 tgcggaagga gaaaatacct gtttgtttaa acaaacaata aaagacctca taaagcggat
    31081 tttgtccgat acgcttgaat tctatattcc aagattcggt taaaagattt attgtttctg
    31141 ctaatttggg ataccaggaa aaacgcagtt catagttatt tacatctgaa cgaaagaact
    31201 gatagaattt ttcaggagct ttgtttggca gttgtgcaag aatatcttcc caagttactt
    31261 cttctttttc ttcttccaca atcagcagag aatttacacc cccaaaatta tcagaaacct
    31321 gatgagggtt gtttttatcg ggaatccaat tgccatccac tataaattta tattgataaa
    31381 ctcctggagg caggtctata gacagagtgt atattccgcc aatttcctcc agaggaataa
    31441 tttcccagga agtaaaatca cctgctaaac cgactgtatg ttttccggca gttaagggtg
    31501 aatagctaaa atgttgataa ctcatagcgc ctgtcttttg gttgttttct ttttgaggtt
    31561 atttttacgg gctttgtctt ccgaattcat ttccagtagc agattttgtg tttcttcctt
    31621 atctttttcc aattgctgtt ttagatgcag acctgttgcc gattcaggac aagccataac
    31681 ttcttcagga gtgccagaac agattatttt tccgccttca tcgcctcctt caggacccaa
    31741 atcaattatc caatccgcgg attttattac atccatatta tgttctataa caataatcgt
    31801 atttcccata gctacgagtt tttttagaac cttcagcagt ttgttgatat catcaaagtg
    31861 aagtccggtt gttggttcat ccaaaacata aagtgtattg cctgtagaaa ccttgctcaa
    31921 ttccctggaa agttttattc tttgtgcttc tccaccggaa agagtggggg agggctgacc
    31981 cagtttcaaa taatccaatc ctacttcttg caaggttttc agtttattac gaatagcagg
    32041 tatggcatca aaaaactcaa ttgcttcctg cacatccata ttaagcacat cagcaatgtt
    32101 ttttccttta taacggatag aaagtgtttc ctggttgtag cgtttgcctt tgcagacctc
    32161 acaggttaca aaaatatctg ccataaagtg catttctatt tgttttactc cggcaccttc
    32221 gcatgcttca cagcgtccac cttttacatt gaaggaaaat ctaccgggag tgtaaccccg
    32281 cattttggaa gcaggcaact gagcaaaaag ttcacgaatg ggatcaaaca atttaacata
    32341 agtagaagga ttggaacgcg gagttctgcc gatgggttgt tggtctatag taataatttt
    32401 atctatgtgt tcaattcctt taatttcatc cattttacca accttctgag cagtatgata
    32461 aaaatgattg tgcagaaaag gagaaagggt ttgattgatt aagctgcttt tcccgcttcc
    32521 ggaaacgcct gtaacgcaga caaaaagtcc caaaggaata tccacattca aattcttcaa
    32581 attattatgc cgggcattta tgatactaag cattctgcca tccggtttaa ttcttttttc
    32641 cggaacagga atttttaagc gtcgggaaag atatgctccg gttaatgact gcgggttttg
    32701 caaaattgtt tccaaagttc ctgtggcaat tatttctccg ccataaattc ctgctcttgg
    32761 tccaaaatca actatctgat cggcagttct aatggtatat tcatcatgtt ctacaacgat
    32821 tactgtatta cctaaatccc taagctgcaa aagcatagcc acaagtttgg caacatcgcg
    32881 ttgatgaagt cctatggttg gctcgtctaa aatatacata actccaacta attgactacc
    32941 aatttggctt gctaaacgaa tgcgttgcgc ttcacctcct gaaagagtag gagaccttct
    33001 atccaaggtt aaataatgta aaccgacatt catcagaaaa cccaaacgat tctttatttc
    33061 tttcagaatt tcttccgcaa taatcttttc atttcctttc aactgcaatt tattaaaaaa
    33121 attatatgcc tcacgaatgc tcattgctgt aatttcgctg atatttttac cggctatttt
    33181 aacagccagc gatgccggtt tcagtttatt tccattacag gcaggacagg gcttatcact
    33241 aatatacaga agataataac gcctcatttc ttcagaggtt gtttcgtgca ttctcctttc
    33301 cagctgagga ataattccct caaaacgcat cataaattca cccctgccat tttcattctg
    33361 ccaagcattt ttaaattttt tccccttaga accgtaaaga ataagctgtt tcactatttc
    33421 cggcaattca taccaaggag tggaaagaga aaaatcataa gccctggcta aactttggac
    33481 tgtgtttaaa gtccagctgt gttgttttgc ttccaaacga ccccaggggg caaccgctcc
    33541 ttccaaaata ctcagttcgg ggtctgttat aaccttatcc ggatcaaatt ccagtttata
    33601 acctaagcca ccacaaagag gacaagcgcc aatagggctg ttgaaagaaa agctttgagg
    33661 ggaaagttct tcaaaaccga tatgacaatg cgggcaagca ttttgagctg aaagaattgt
    33721 ttcttgcttt tcttccggat agtcaatttt tacaatgcca tctgcaagtt tcaaagctaa
    33781 ttccaaagaa tcggaaagac gactttcaat ttcttccttg attacaatcc tatcaaccac
    33841 tacatctata ttatgcttac tctttttatc caatacaatt tcttcttcca aatttcttaa
    33901 ttgaccattg atcattactc gcacaaaacc ttcctgacga agcatttcca gttcatcttt
    33961 atgttcgcct ttacggtttt gaacaatggg agctaaaatt tggagtttgg tgccgggttt
    34021 attttgcatt aaatgggaaa tcatctgatc aattgtctga gaaccaaccg gtcttccgca
    34081 attgtaacaa tattgttttc cgattctggc atacaaaacc cttaaataat cgtaaatttc
    34141 ggtagcagtt cctaccgtgg aacggggatt tttactcgct gatttttgct ctatagatat
    34201 cgcaggagaa agaccttcaa tatagtcaac tttgggtttt tccatttggc ctaaaaattg
    34261 tctggcataa gcagaaaggg attctacata tcttctttgg ccttcagcat atagagtatc
    34321 aaacgctaag gacgattttc ccgaaccgga tacgccggta aaaaccacaa gattattacg
    34381 aggaatagtt aaatcaatat ttttcagatt atgttcctgc gcacctttta taataatagc
    34441 tgtcttcaaa tttcacctca tcgttagcca aatctttttg tgtctgacta attgtcaaat
    34501 gaaaatcgga agaacgaggt cctcatcggt ccctggaaat tctttttggg ttggcaagga
    34561 cagcaaccat cagaacgaaa aaattgaaaa ataaaacatc attgaatcat aatacttaat
    34621 cgcttgttat atagtagatt aagtaacgac tccgtaacac ttccgtaaca cttccgtagt
    34681 tccgttacgg ggttgttacg gaagtgttac tttgttataa gaagtaaaag agaacaggga
    34741 ttgtgctatg cctataatag gtttctcctt ttcactattt cacttttccc ctttttccgc
    34801 tatagctatc aaataaagaa agaacttgac taatacttta tttattgatt ttaggaattg
    34861 aaagatttta tattggagaa atagatgagt agcacccctc gtggtgaacg cttgaatatt
    34921 gctataatcg gaaaaagaaa tgccggtaaa agcagtttga taaatgccct ggtgggtcag
    34981 gatattgcca ttgtatccgc tgttccaggc acaacaacag accctgttga taagcgttat
    35041 gagctcttgc ctttgggtcc cgtaactttt tttgacactg ccggagtgga tgataccggt
    35101 gaattaggtg aaaaaaggat taaggcaacc caaaaaatac tctatcgtgc agatatcgct
    35161 atttttgttt ctgacggcaa tgccttcaat aatgccgaac tgaaaatgat agaaaagatc
    35221 agggaaatgg aactgccaat gcttattgta tttaacaaaa aagatataat accggtatgc
    35281 caggaaaata ttgccttctg ccaaaataat tccctgccgt atattagcgt ttcttccgtt
    35341 accaaagagg gtatagatga atgtaaagag aagcttattc agctctctcc caagtatttg
    35401 aaagaaaaca gaattattgc cggagacctt gtagaaccta aaaacagtgt tattttagta
    35461 actccgattg atacttctgc tcctaaagga cgccttattt taccgcaggt tcagaccttg
    35521 cgtgatttgc ttgaccggaa ctgtctcgtt acagttgttc gggaaacaga gctaaaatcc
    35581 gcattggaaa agacaaaaga acccccttct ctgataataa ccgattccca ggtaatagaa
    35641 atggtggcaa aaattgttcc tgaagaaatt cctttaacta ctttctctat cctttttgcc
    35701 cgctataaag gtgaactgga tatccttctg gaaggcataa accgccttaa aacactgcaa
    35761 gatggagata aaattttgat agctgaggcc tgttcacatc atattcaaaa agatgatatc
    35821 ggcagggtta aaatcccgaa ctggatgcaa aaattcagtc aaaaaaacct gaattttgag
    35881 ttctatgcgg gacacgattt tcctgaaaat ctggaagaat acgctgtttg tatccattgt
    35941 ggcggatgta tgattactcc gatggaaatg aacagaaaaa ttatggaatg ccgtagacgc
    36001 ggcgttcccg ttactaatta tggtatgaca atatccttgc tgaaaggcgt tttagaccga
    36061 actattaaac cccttttaaa agctaaggcg aaacaagcac aatgaaaatc tccgctttgc
    36121 aatttgcccc acgtttactg gataaagaat acaacctgaa aaaaatgaaa cagttgctta
    36181 tcggactgaa aacagacctg gttgtcctgc cagaattatg taatagtggt tatgttttcg
    36241 ctaataaaga agaggtcttc tccgttgctg aagaagcagg aaaaggtgaa acatactttg
    36301 tgttaaatga tattgccaat agcattaact gtaatattgt ttatggatat cctgagcttg
    36361 atggcaataa cctttttaat tcctgtatgt taattatgtc tgacggcaga ttttataact
    36421 atcgtaaaac ccatcttttt aatcgggaaa aactattttt cagtcccgga gataccggtt
    36481 ttcaggtttt taatattttg aatggagtaa aaatcggaat gatgatctgc tttgactggc
    36541 aatttcctga ggcagccaga actcttgctc ttttaggagc tcaaataatc tgccatcctg
    36601 ctaatttagt tctgccctgg tgtcaacagg caatgaaaac ccgttgttta gaaaatcggg
    36661 tatttgctgt tactgccaat cgtataggca cagaattaaa tgcaggagaa acagttagct
    36721 ttactgggca aagccaaata ttaaatacca aaggtgaagt tctttcctct ctgtcagtta
    36781 gcgaagaaag aactataacc gtagaaataa atcctgaaga agcaaacgac aaagccgtaa
    36841 ctgaacgcaa tgatgccttt aaagatagaa gaccagagtt ttataaatta tgaataaaaa
    36901 taaattaaaa gccagaattg accgcttaag agccgaaata gaacatcata atcggctcta
    36961 ttatactctt gccaaacccg aaatcagtga ttatgaatat gatatgctgg tgaaggaatt
    37021 acaggaattg gaagcacaat atgcagaagg tgcaattgaa gaatcaccta ctcaaaaagt
    37081 tggaagcgac cttactccag gcgctaaaac cattcctcat aaagtgcgga tggcaagttt
    37141 ggaaaatgct tattctttgg aagaggtttt ggctttcatc aatcggacaa ataatgattt
    37201 gggttatgcc agtgaatact gtgcggaatt aaaaatagac ggatttagca tcaatcttta
    37261 ttatgaaaac ggaaatttta tttatgccac aacccggggt gatggtttgg aaggtgaaga
    37321 tgtaacagaa aattttcgtt tgctcccgga tgttcctttt accattgatt ataaaggagc
    37381 catagaaatt agaggcgaaa tatatatacc cgtgcaggat tttctggcgc tgaatgaatt
    37441 ccgtagagaa aatgaattga agacctttgc taatccgcgt aacgcagctg ccggctcaat
    37501 taagctgaaa aatagagagg aagtgaagtc ccgtcattta aaggcattgt tttatgcttt
    37561 gggctattat gagacacctc tgcagtataa aaatgaaatt atagataagc aaactcggtt
    37621 attggacttt ttggatgaac ttggtttccc aaccagtaaa agaactttat gcaagaatta
    37681 tcaggaagtg gaaaaattct gcctgcaaat ggaacaggaa cgttatacta ttccctacga
    37741 tattgatggc attgtgatta aggttaatag tttggaagac cagaaaaaat tgggctggac
    37801 ggctaaaagt cccaaatggg caattgccta taaatttaag ccggaagaaa aagaaaccgt
    37861 tttggaatcg gttagttttc aggttggcag aaccggtgca gttactcctg tagctaatct
    37921 aaagccggtt tttatttccg gaagcactgt ttctcacgct actttacata atgccgatga
    37981 aataaaacgg ttggatttgc attatggcga tactgtttta gtggttaaaa gcggagaaat
    38041 tatccccaaa atcctttctg taaatgttag taaacgccct gtaaatgcca aaccggttat
    38101 ttttcctgct aactgtcctg tttgtaatgc acctttacaa cgcgatgaag atggcagtat
    38161 aaactactgt cctaatgcca attgccccgc tcagatacag cgtaaactgg aacattttgc
    38221 ttcgcgtgaa gcaatggata tttatggctt aggtagttca ctaatagcgc gtttgataga
    38281 aacaggtcag attaaatgga tacctgatat ctatcatctt gattataata aaattgcaga
    38341 aatggaaaga atgggaaaaa aatctgccga gaacttgcaa aaagccattg aagcatctaa
    38401 aaccaaaaat tttgacagag ttcttttcgc tctcgggata agacatatcg gctctattac
    38461 tgcccgcact ctggctcaac actttaaaaa tattgatgct cttattatgg caaaggaaga
    38521 agaactttta agtgttcccg atattggttc cattgttgcc cggtccatta tcggtttctt
    38581 ctcccaaaaa agcaatcggg aattgattga ctctttgaga aaagtcggat tacaattcac
    38641 ttatataagc ccgcaaagct ctgctatctt agccggaaag acctttttaa taacgggaac
    38701 tctgcccaac tatagtagaa aagatatgga aacactgatt acggaacacg gaggaaaaat
    38761 tttaagcggt gtaagtaaac agctgaatta tcttatagta ggtgaaaatc ccggctccaa
    38821 actggataag gcacgccaac tgggaacagt taaaattatc agtgaagcgg aacttttgga
    38881 tatgctgcaa tgaaaatatt gaaacgctat atcctgaaag agcatatcgc accttttttt
    38941 attgctctga tggtagttac ctttgtgctg ttgagcgaca gaattattga cttaatgaat
    39001 ctgataattg aaaagaaact gccctggcaa acggtttttg aggtctttgg
//
LOCUS       DAOPFL010000005        37954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_997_length_37954_cov_108.265706, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000005 DAOPFL010000000
VERSION     DAOPFL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 37954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37954
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_997_length_37954_cov_108.265706"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="PLG69_01025"
     CDS             <1..1397
                     /locus_tag="PLG69_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPI42121.1"
                     /translation="NKHLCLTPMHLYIYWIKISNKKGEKMLRGNEIKPAEGKLGVMLV
                     GLGAVSTTFVAGIQAIKKGISEPFGSVTQMSTIRLGKRTENRTPLIKDFVPLANLEDL
                     EFMAWDIFEYDAYEAALKAGVLEKELLDKIEPELRSLKPQKAVFNNNYVKKLHGTYVK
                     TGINKMDLAQQLMDDIKRFQEKNNCSRLVMAWAASTEVYLESDPVHNTLESFEQGLRD
                     NHPAIAPSMIYAYAALQMGIPFLNGAPNLNLEIPALRKLAEEKKVPVAGKDFKTGQTL
                     MKTIIAPGLKARMLGIHGWFSTNILGNRDGEVLDDPESFKTKEVSKLSVLETILEPEK
                     YPQLYKDLYHKVRINYYPPRGDNKESWDNIDIFGWLGYPMQIKINFLCRDSILAAPVL
                     LDLILFIDLAQRCNMSGVQEWLSFFWKSPMTAPGLYPENDLFIQQMKMKNTLRYMMKE
                     DLITHLGLEYYNEM"
     gene            1459..2565
                     /locus_tag="PLG69_01030"
     CDS             1459..2565
                     /locus_tag="PLG69_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPI42122.1"
                     /translation="MKKIKLLFRIGYAYHKSAFDPIIDLLLNNDKYDVWFSLDMEKIK
                     YFIFEFPYRNQIIEDWKKLGYRFTNETKGFDIVISGDTLRNAKDYGKTLLIFLNHGTG
                     IKNILYRNLARSPGVKYQIFVEGQHRVDSLLKCPYLGKSEVHLIGLPKLDYYFQGKFN
                     REEVLQRWGLNPAKKTILFAPTYKPTCMYEIKDDIFEQTRDYNLIIKLHPYSWMGKYA
                     PHKQHRIFEKRVKKYPHSVLLPFTEFNIVPYYAAADTVISEASSTVFDFIALNKFGIV
                     YDLACDKLNHSDGQPLLEIDNREFLKGAYPHIQNGKQLPEAIATALNPTLDMIAKADE
                     YRQRYFYGLDGKAALRFVEKMEELYYEGGHENGV"
     gene            2569..>3255
                     /locus_tag="PLG69_01035"
     CDS             2569..>3255
                     /locus_tag="PLG69_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPI42123.1"
                     /translation="MKGVIISAGIGSRLWKTTKQVPKTLLPYKNGTILSTIIEQLKQA
                     GITELIIVVGFKKNYIVDYLQKNPPALPVTFVENLEWEKGNALSVYKVKNYIIAEPFL
                     LSMADHLVKVEALKQIVDSPERINLLLVDPFITENFDLDDATKVKTENDYIIAIGKEL
                     TDYNAIDCGIFRLEPDFFSAVEKAVAKGKDSISNAIAELIPLKRIKSITLNKANQWLD
                     IDTPEAYQFAQ"
     assembly_gap    3256..3355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3356..4071)
                     /locus_tag="PLG69_01040"
     CDS             complement(<3356..4071)
                     /locus_tag="PLG69_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPI42124.1"
                     /translation="MNKANFYRTELLVGQQGMQKLARTCVAIIGLGGVGSYSAEALAR
                     SGIGKFILIDFDVIEATNINRQILALHSTIGKPKVEIMQQRILDINPQADVIIYKEFL
                     DKENQERLLAGADYCVDAIDSLGSKICLLEYLIKNEGKFISVMGAGNRLDLFQVHLST
                     IDKSYNCPLARRVRKLLSKRGIQGNFPCVYSSELPLVPEKNNDISEEGVIDRAKQKQV
                     IGSISYLPAVMGLMAASYVI"
     gene            complement(4064..4789)
                     /locus_tag="PLG69_01045"
     CDS             complement(4064..4789)
                     /locus_tag="PLG69_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPI42125.1"
                     /translation="MAFIIDAHCHLANLDVLMPVKPLMDETKKFGLDFWLSSALTKSE
                     IAWHQKNPDKRIAYSAGIHPYYKECDLELKDIEELCIEKKIWAIGEIGLDRNNPDFAG
                     QLKIFIEQLELAKQYNLPVVLHLVGHISEAYKLLKQYNLSYLVHGYAGSIQGFKELLR
                     LKSFFTISERLLKWDKKELLRAMIDSKRYLFETDITRDYVQEEETNPLLRLNTLISSV
                     AEFAEIPRGKLIEQQQKNFNSYE"
     gene            complement(4799..>5356)
                     /locus_tag="PLG69_01050"
     CDS             complement(4799..>5356)
                     /locus_tag="PLG69_01050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPI42126.1"
                     /translation="PSEKDRQKAEKLLQPLSGKTIIAVAPGSIWQTKCWEPNSYISLC
                     QKLLDSGYAIVLIGGESDKLISNEIENALGKNNTNLINLAGVTNLLQSAAVIAKCSLM
                     ICNDSGAMHIANAMQTRVFAFFGPTVQRFGYYPYREGDRVFEVELECRPCGSHGSKKC
                     PQKHHNCMKQIEVEPVFKAVETTLR"
     assembly_gap    5357..5456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5457..6223)
                     /locus_tag="PLG69_01055"
     CDS             complement(<5457..6223)
                     /locus_tag="PLG69_01055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42127.1"
                     /translation="MKVLHIDAEKHWRGGQQQAVYLYEGMLKSDYKCGFVCCPGSQLQ
                     QYLEKKRLPYHCLMFRGESDIISAFNLARLCRRYSYNILQLHSSHSLSWGLWAKFFYP
                     SLKLVATRRVDFPIGKNPLSAFKYKTKAVNKIVAVSDNIRIVLISCGVPSEKVVVIYS
                     GIDLHKFDTVQVPTDFRQTWNIPEKSIIVGTVAAFAGHKDYPNFLKAASLAVQENPNL
                     FFMAVGGGDLLSEMKNIAQELGLQGHIAFTGFQKEVG"
     gene            complement(6220..6966)
                     /locus_tag="PLG69_01060"
     CDS             complement(6220..6966)
                     /locus_tag="PLG69_01060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPI42128.1"
                     /translation="MEQLSAVLIVKNEEQNIARCLKSISWVDEIVILDTGSSDATVNI
                     CKQLGAKIYYLDKWEGFGKAKQQAVDLATFDWILSIDADEEISSELQKELQELKNKRF
                     ENTAWRLKRRSYYLGKPIHFCGWQNDAPLRVFNRKQGKFNEKLVHEGVQTSQKVKSCK
                     HYLYHFTNPTAEVHYTKMRLYGELSTKQLYAQGKRANEFIAWVRGIFKFLKMFIFQLG
                     FLDGAKGFLLCKNSAWGVWYRYHLLGKMKK"
     gene            complement(6967..8118)
                     /locus_tag="PLG69_01065"
     CDS             complement(6967..8118)
                     /locus_tag="PLG69_01065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42129.1"
                     /translation="MNSLERVLGYPFYYTGVLADKILHWNNPSSLFFFFPHFHTGGAE
                     QVHLDILRIFADQKPFIIITKNSRNKTHYQAMQKSGRLIEIYKYLNNTNRFIVKNFFY
                     GFLASMINRHPKSVVISANSTIFYELSHYLTKSYLINILHGFYGINPKIWLLATPKIN
                     KRIVVSQAVYDEVQQYYKANGWEEKYNSRLKLIYNSIPLCDDISPKDYQAPLKIVFIA
                     RQAKEKRFYLYQQVARKIIREFPKIEFYCIGDHINTEPVINLGEICDRQKLYETIKDF
                     HILILCSISEGFGLVIAEAMTCGLVPLATDVGGVRESFQDGINGFLIKSKEEQEIIDE
                     FISDIKVLNSSKKLLQQMSNSAREYVQEHFNYDRFKVEYTKVVEEGRNR"
     gene            complement(8115..8951)
                     /locus_tag="PLG69_01070"
     CDS             complement(8115..8951)
                     /locus_tag="PLG69_01070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42130.1"
                     /translation="MSISLIVPVFNYLNLLEKCLFSVYRQSVHPDEIILSDDGSDEDI
                     VAFYNAQKRKTAIPLILVRQKHLQFRAARVRNNGVSVSKGDVLVFLDQDIVIPQDYLK
                     CIMANLNRRRFLSGYPVRLSKEQSDKLTFEIIEQNNWNTILTAEQKRKIQKQYKKDFL
                     SYLLFRYLHLGGHGAKLRGGVSAIFKSDFELVNGYDENYIGWGNEDDDLGRRLQELGK
                     SGFNFVSNQIPLHLYHTHYHDQGERKNANYSERCKKKISHRNYRCQKGLSSIRNDLEI
                     LE"
     gene            complement(8948..9823)
                     /locus_tag="PLG69_01075"
     CDS             complement(8948..9823)
                     /locus_tag="PLG69_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42131.1"
                     /translation="MSILPICDEFVIAIGKSDPDDDTKERILALKEPKIKIIDTEWDP
                     KLCKRGVINAWQTDIAKNQCQGDWLFYLQADEVVHEKYLPVIKKRCEELLNDKEVEGL
                     LFYYKHFWGDYYHYQNGHGWYPFEIRIIRNDPHIHSYQSAQSFRYFEYYDNPRQEKGT
                     RKLKVAKVDAEIYHYGWVRPPQLMQNKRRALDSVHWGKEKADAYYNNAPQYFDYGPLS
                     LLAVFNGTHPKVMQERISCFDWQDKLQLTGKPNPYREPHKHETFKYRFLTFIEKHFNG
                     GKQIGTFKNYILLKR"
     gene            complement(9926..11836)
                     /locus_tag="PLG69_01080"
     CDS             complement(9926..11836)
                     /locus_tag="PLG69_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42132.1"
                     /translation="MKRKIKNRWNNIILIFRMMLRYWHYMLAGFISMLLFALFNGVSV
                     TLVIPLFDYVFKPNKINLLYTDFSSFLVALGEMLKQHISSSGGFFPAIQNYSALWEDA
                     KVLMLQTDSLSLLYLLCIAVFLVILFKNVFYLLHKIFFNSVRGRTIRDIRNYMFSRYL
                     NQSLTFFSRHRIGDAIVRMVGDVDIVSEQLINSFVNVIREMITVLVFARIAYLLNPVL
                     LLYSIIILPVFSLTIGLLGKKIKKYSKRIQEQLSNLFSNVEEVLNSMKIVQAFRHEQY
                     ERKNFEAINNKHYHMWFKAQYYGTLSTPISELNTAITGIIVIIIGGKMILTPGSSFSL
                     GDFTAFLFALFSMLHPLKVITQVYTDIRKALVSLDRIGLVMNQESRIQDKPDAITKET
                     FDSEITFENVTFGYKENKPVLKNFSLTIPKGSKVAFVGASGGGKTTIANLMNRLYDVT
                     YGAIKIDGIDIRDIKLDNLRKFFGVVTQDSILFSKSIKENIAYGSQEEVSDEQIIKAA
                     QIANAEEFINEMPNKYDEVLGSKGSDLSGGQKQRLCIARAIVGDPPILIFDEATSALD
                     TDSEQKVQMAIDKATQNRTVILIAHRLSTILKADKIVVLEKGKIVGMGTHQELMQNCP
                     RYQHLYNLQQGK"
     gene            complement(11888..11964)
                     /locus_tag="PLG69_01085"
     tRNA            complement(11888..11964)
                     /locus_tag="PLG69_01085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11928..11930),aa:Arg,seq:acg)
     gene            complement(12488..12760)
                     /locus_tag="PLG69_01090"
     CDS             complement(12488..12760)
                     /locus_tag="PLG69_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42133.1"
                     /translation="MKFKKLIPTFIPFAYELIHIYKRNAKHDQNIRKKDRTAEKIGSL
                     EHLMVRLEKKVQQNREIYIKSFNKIKIWLAINSILLIAILVKLFIG"
     gene            complement(12769..13614)
                     /locus_tag="PLG69_01095"
     CDS             complement(12769..13614)
                     /locus_tag="PLG69_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42134.1"
                     /translation="MKKLLFVCLLLIPSVLLLAVFDDYQPSAIARGMGGAYTAVSKDA
                     NAIFFNPAGLINTTFSAKLSFSQLYNQDFSELKTVALGYKLPKNLGTVAIGARAMDVD
                     FEDVNLMSEQVYSLAHSFILLEDIHSRISFGYTGNLYLLSMDGEDDDTALGIDLGALA
                     VLHSRTHIAFAVTNINKATMGDENQIDLPSKLALGIAYVPYEKVTTSIEVKKDFAKET
                     EFMGGVEAHLFEPLAIRFGVHQNPATYNAGLSIYIQNLEVDYSFTYHSVLPGTHYFSL
                     GYKFK"
     gene            complement(13623..15056)
                     /locus_tag="PLG69_01100"
     CDS             complement(13623..15056)
                     /locus_tag="PLG69_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPI42135.1"
                     /translation="MKNYILTFLAIFLVAGISAQTVLSCHDIQYTTDPTGNSPYYTQT
                     VTVQGIVTHIIPNSAFYIADPEGGEWSGLYVYHRNTSNVVSIGDLVKLTGTVDEYYNL
                     TEFTNVSAYEIISQGNPLPPFLELSTADMPSGTNHPENEKYEGVLVRFQDVTIKSTPD
                     SYGQFLIADHSNIWAMVDNGLYAIPASSIVVGDSWYIIQGIVDYHSSAGFKLNPRNAS
                     DMIKQDTIENSIIKIVRTDPTTEPTLNSDVSMNLISTKIKSEWGVMSYSVTFKVNPTK
                     LIFSGLDIDSTLTREMPDFQISAGGDTISWTYFSQDGIIYPDNDALLIKILVRPLVYG
                     ENIIDLVSFKYNETEVNNLIDGSVIVKVPKKIAYLNIGKQGDKKNIFNPEMGEEIIIS
                     YGTCTGYLSKAVIRIYDAQGRLVYTPVHTNITSATGIQEFKWNGRDANLKLVPPGLYY
                     CHLEVTDRSSGGKDNTVQPIVIKSALK"
     gene            complement(15057..15623)
                     /gene="lspA"
                     /locus_tag="PLG69_01105"
     CDS             complement(15057..15623)
                     /gene="lspA"
                     /locus_tag="PLG69_01105"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPI42136.1"
                     /translation="MKQPRTQLKTAPAYFIMLLILVLDQLTKTLVRVNMQMYECIPLL
                     QNLFGDTFILIRVQNSGAAFSFGFGSDLVNRIIFICVTVLVVGIIIYLLHYAQHKIQV
                     IAFGLILGGALGNLIDRVLFGPVTDFFSMDFPDFIMERFPVFNIADSSIFIGVVLMII
                     DMLFIKDKPSPLPETGEHIEISEFNKEL"
     gene            complement(15620..16021)
                     /locus_tag="PLG69_01110"
     CDS             complement(15620..16021)
                     /locus_tag="PLG69_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPI42137.1"
                     /translation="MATKKLPPEKLKHYEEVIRAELEESIAYIESINKEQSLGARESS
                     GDLSSYAFHQADQGSDTNLMEQSVMMLESEREKIRQLNEALQRISDGTYGICEFCGEF
                     IQENRLEIIPYATCCVACKEKNEDKKRRQKR"
     gene            complement(16082..16915)
                     /locus_tag="PLG69_01115"
     CDS             complement(16082..16915)
                     /locus_tag="PLG69_01115"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPI42138.1"
                     /translation="MSSYKDTSAWLKTKLPKQPQIAVILGTGLSNMAEVFPLLYSIPY
                     NQIPGFVQSTAPTHKGNLLLCEVEGIPVIFLQGRFHYYEGYSMQEVVFPVRVLSALGI
                     KVLIVTNASGSLRKELEPGTIVQLQDHINFMGTNPLIGMNDDDLGERFPSMNKVYDPE
                     LCLLADKVAFDLGIKTAKGVYIAVTGPSLETKAECSAFASWGADLVGMSTVPEVITAR
                     HSGLKVLAYSIVTNYSNLFHNLAHSQEEIQRIADISGKQLQKLIAELILRLTRTFSLT
                     S"
     gene            complement(16916..17500)
                     /locus_tag="PLG69_01120"
     CDS             complement(16916..17500)
                     /locus_tag="PLG69_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPI42139.1"
                     /translation="MPLFPLDIRHQEFSTAMFGYSKKEVRAFLDQIASEVEAFQKQLE
                     KEIARQEQKQNEVKQEALQAGSAVEELKRREELISRTLVFAEKTKADIIANARKEAEN
                     IIKEADLKAKRAIQEAKQYLSVLEHQYLQLKEQKRQFLMQFKVELQSFQDRINKDPLL
                     SKDSELRLDSEFHQIKEEIVPKPENTETQNEKNK"
     gene            complement(17514..17786)
                     /locus_tag="PLG69_01125"
     CDS             complement(17514..17786)
                     /locus_tag="PLG69_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPI42140.1"
                     /translation="MIYSTSLTGSILLFAVRIIQIYNILIIARVFASWIIRNPYNRLY
                     HFLISITEPVLGPIRHILPPMMGLDLSPIIAFFILDLLSRLLTSLI"
     gene            complement(17783..18346)
                     /locus_tag="PLG69_01130"
     CDS             complement(17783..18346)
                     /locus_tag="PLG69_01130"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPI42141.1"
                     /translation="MLNIVNQKTGWIEVICGSMFSGKTEELIRRIRRAEYAKQKVLVF
                     KPVIDNRYDANNIVSHSQMQAPSIPINAPGEIYSYLDDDVKIIAIDEAQFFDPSLVGI
                     CNDLADRGYRVIVAGLDQDYKGEPFGSMPQLMAIAEYITKNLAICVVCGNPANRTQRT
                     IHKDEQILVGSTDAYEARCRNCHEVLD"
     gene            complement(18907..19188)
                     /locus_tag="PLG69_01135"
     CDS             complement(18907..19188)
                     /locus_tag="PLG69_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HPI42142.1"
                     /translation="MKQLPKLLAVVLLLVIISSCTINKPICATSNPLGEKQGVYTQIG
                     ILFFQPVANSDAAIAKAAENGGITNISTVDYNTTWLLFLMKYQTIVTGN"
     assembly_gap    19573..19672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19673..19960)
                     /locus_tag="PLG69_01140"
     CDS             complement(<19673..19960)
                     /locus_tag="PLG69_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPI42143.1"
                     /translation="MRENVAVPKMSNEHLRYLSKLKQKKFRLQEGLVIVEGKRTLEQL
                     FAWSIKPLELYLVEGEPTVSAAISYTVSEQGMKRLCDSEQPQKIAGLFSLPK"
     gene            complement(20135..21811)
                     /locus_tag="PLG69_01145"
     CDS             complement(20135..21811)
                     /locus_tag="PLG69_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HPI42144.1"
                     /translation="MKYFLIIFLLTIGIFLSAESWTVLLYMAADNNLAEMGRLDINSL
                     ESVAQPANLNLIVQADFPEGAKRYKIQQDNSEQITSPVLANLGTINSGDPNTLNSFIK
                     WGFNRYPAQRKMLIIWSHGDSWYKDNSGKWICPDDNAQDLISVSGGELVLALSGIPHL
                     DILLFDACSMQCIEVINEIHSFADYIIGSEDLVPQYGFPYEDIIPLFDGDFDELLAQI
                     PELYVSSYLPGQGINPGGALWSVTCSVVKTELVPQFVQQIKNFAISQRFLAPKYFTVR
                     NSCYSMNTGLADIDIRDFLEKAQTVWTSGTQNLLSLWTSIVISSCFTNPEETGNIGTA
                     AIWFPDSRFNFENGWRQYHKLNFSRSRWFSFVNSAIGNDTFPPETPILISQNLIYNTL
                     QISLQANPDPDSLYYEITNAGGEHFQYFYTSPDNAVFTIMLQISQAGTYQIRAIDQSG
                     NKSQPLIGNYTYSNPQASIIINPNPVSGTSLAVLRWWASEELAGKIQLEIYNLKGQKV
                     INRYLGEVIAGEGNFLLSAEPKFRALPSGVYILRLHLGNRKFTKKLTILY"
     gene            complement(21808..22887)
                     /gene="buk"
                     /locus_tag="PLG69_01150"
     CDS             complement(21808..22887)
                     /gene="buk"
                     /locus_tag="PLG69_01150"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPI42145.1"
                     /translation="MNYRVLAINPGSTSTKIAVYDDSTPVFEKTLRHDPEELNQFGDL
                     FNQTDFRKALVMEAMEENNVHPQSLNAVVGRGGLVRPVSGGTWKVNEAMLRDLKDITI
                     WGRSHASNLGAFLAKAVAEPLGIPYFIVDPVATDEMEDIARLAGIPEIEHKSLFHALN
                     IRYICRLLAEKLGKNLTEVNFIGVHMGGGISVAAIKNGRVVDVNNALLGTGPFSPQRA
                     GSLPIGDLIEMCYSGKYTKKELTTYLSKGAGLMAYLGTDSGIEVGKRVAEGDQKAKLV
                     LDAMCYRISKEIGSCSAVLAGKVDGIYLSGGLAYNDYIVNFIKEHTKFIAPIYLYPGE
                     KEMEALCQGGIRVLNGTEEAKEYPY"
     gene            23068..23427
                     /locus_tag="PLG69_01155"
     CDS             23068..23427
                     /locus_tag="PLG69_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPI42146.1"
                     /translation="MTPKEKNETQPADTHICDEECQHEQFVIPVQSEDGSTQNYIPIG
                     HIDYEDKQYIALVPEGEDTYDIYQMVGEGDIVTFYPIEDEELYDLIADKFNELFLFEM
                     DKMGLLDEENFLDPEED"
     gene            24129..26591
                     /locus_tag="PLG69_01160"
     CDS             24129..26591
                     /locus_tag="PLG69_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42147.1"
                     /translation="MKRKIVFGFICLFLCGSCWLTRKIDTHQFALKEMTQFATPLNGW
                     ITAGLKEEGEDFFICHSQYDENLYFVLVNDIQGKTTSQINLPSKIIDLNTLIDKRNND
                     QWLFISSNDSDSLYLMGVKYIWQIPLQRENKYFSSLERTDWAKGRVDYIYNGSIYPIF
                     LEDIDNDGKMDLLCQLIDFFTAKPRGLILYDFETGFIKWRYELPCNPRSILWDDFDGN
                     GSKEIIVSNDALKNTTEIINDVDDCSGWIIVLSAKGDLLFKEKEFSGYGRIYLDAADV
                     KQDGIKEIYAVNCTWGSEKHFNIASVFNWNGKRLIKNRTLELPSTLESNQFPNFLQQM
                     DSRSDYYLHLVDTKAGLLILDTNLNPVKHNYKDFVNQIWDIGDLDRDGNKELFLQKKD
                     GSLEILDKNYYRRARIKNPFPDTIIYNANIVKTGFETKPLLLVGTNKQINYYEYTSLS
                     AFTILLSLLRNYALLLFILAALFCIILIIRNMYHKKNTLKLIDSWEQGILKVNKKGII
                     KYHNFKIKELANDFELLKRKKLEHCFPALAKPFQEFCNANLKTGSYIGTLNPNPQSKV
                     YQVYFIRMTSYRKFYYLVIRPYTEEGAIAEKMEWAETARRLSHHVRRYITNILLALET
                     LNTDTDPTRNKYYQIIRSEIDKVRVFTHAFQRFTELQDYQLELQDIIPSIEHCLSKIQ
                     FPENIQLIKNWSLASISAYIEPIRFEEALTNIVNNALEAMPEGGTLHISVKEFPLASS
                     PQGQLRVLIEIEDTGKGIPQKYLEDIWKPFFTTNQSGTGIGIPETKKIIDSMGGLMNI
                     QSEENIGTVVTIWLKGDSNVRN"
     gene            26578..27927
                     /locus_tag="PLG69_01165"
     CDS             26578..27927
                     /locus_tag="PLG69_01165"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPI42148.1"
                     /translation="MLEISILLADDDLIFLQLTSDFLGKNGYAVDCASNIEATKTLLQ
                     KNNYDIMMLDMCFPALYDGFNLLDEVHENYPDLPVLMISGSGHIPDAVKAIQKGAADF
                     IEKPIDTTHLILRLERIYDGIVTKKQMQSLRLATIGMQGNSAIMQNIYDDICKAARFD
                     CPVLITGETGVGKELAARAVHRLSKYSAKNLVSINCASIPKELFEAELFGYESGAFTG
                     AVKAHRGYFEYADNNSLFMDEISQLPLEVQAKLLRVVSENEIQKIGGKVQKVNVRIIS
                     ATNQDLEKLTAEKLFRKDLLYRLNTIEINIPPLRKRIEDIPVLAEFFMNEFCTRNDIP
                     PKPISPSAVSWLCKQPWEGNVRELKNAVERAVVFSKNDHLTMVDFTTPSESDITDREY
                     GSLRKTITNFEKVYIENVLRIYNYNVRQAAYHLQMDKSNLFKKISALKITLPNHKPI"
     gene            complement(28298..29104)
                     /locus_tag="PLG69_01170"
     CDS             complement(28298..29104)
                     /locus_tag="PLG69_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPI42149.1"
                     /translation="MYKSIKQQQLEQRILKLLSGKALKTAEWLFANPELQAGQEYANI
                     VSIKRLGFNDHGPVHMRKATLNAIKMFNLLNRSGIKMNLEKEGAGTADDSLTAVILAS
                     LLHDLGMAITRESHELLSIQLAIPYIDTVLNTLHSENEIVLKTAIRSLAIEGIFGHMA
                     THKIHSLEAGLVLIGDGSDMEKGRARIPTMLSSQTRVGDIHRTSASAIQKVKIFKGQE
                     KPINITVEMNASVGFFQVEEVFFPKINISPVKPYIELYAGVTDREMLRYL"
     gene            complement(29117..29569)
                     /locus_tag="PLG69_01175"
     CDS             complement(29117..29569)
                     /locus_tag="PLG69_01175"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPI42150.1"
                     /translation="MRPFDTLTLMQFIWAFLAILGFSIRSNLKGIRILFTALSGGLCW
                     AFYLIILYYSKSMLFSVFVAIILVCIYSEIVAPRLKTPVSVFVTCAIIPLVPGRGLFN
                     SMQLYIAGDNLHASKAILQTLLIAGTISIAIAIVSSVTNLINKLMNRF"
     gene            complement(29569..30360)
                     /locus_tag="PLG69_01180"
     CDS             complement(29569..30360)
                     /locus_tag="PLG69_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPI42151.1"
                     /translation="MPKVSSQQVLELALEIGRIILQSGGTTNRVELMMKKVCNCFGYP
                     ETESYVTPTGIFISIKDEKGNIFTSIKRIENRRIDLGKITRISTMVNCMEVKALSEQE
                     LQEQLVNFNSELKKIDNENPWSPWIRDLCGGVTSGFFCLLFGGSWAEFAVAFFIGAVV
                     TFGLKYLEKLAFNNFLLNAIAAALIVMLAKTLDIFIPYIRLDNIIIGGIMLLVPGLSI
                     TNAIRDTMSGDLVAGTARAVEALFITVGIVAGSASMLKIWSMWGF"
     gene            complement(30347..31957)
                     /locus_tag="PLG69_01185"
     CDS             complement(30347..31957)
                     /locus_tag="PLG69_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42152.1"
                     /translation="MQKNVKEALLLDKLFALLETNGEVMESVKHQKLNKKHYSFEELK
                     TQNRNTENLLKAIKESRALEARLESIFSELSLLPIKHLKTTERLELNELFEIKSFLFS
                     YLHLQEILQEHKLNHQHPLPDMQKMFSLLDPEGNKLPTFRIYPSYSSVLKKLTQKQFA
                     IAKRLKEARKRDLEKAKQELGMPTLKEEFTLSRNRKEQLEAILASKYFIVVSEGLANY
                     NFRLADSKETADLKAELHQLSIELTEEENKILCSLTTQIQNAFSNFELAYNSAGEYAW
                     DFCLAKFALHYNCCLPNITPSFEGIYLTEAVNLPLKLFLETLQRQYQPLNITMSKKDN
                     LITGPNMGGKSTALITIGQMCILASLGIPLPAKKAKLPLYDEVYYNHDAGENSETLSS
                     FAREVVSLSNMLLRKGNKLVLLDEFAKGTNPEEGEAICVATLKYLINSNNTIISATHF
                     SAPAELAGLAHFTIKGIDEQGLKRLEKMQDSNLETRLALLSEAMDYSLLPVSEKTAPP
                     KCAIRIAGILGLPAEILQQLPKEKNAQS"
     gene            complement(31986..34295)
                     /locus_tag="PLG69_01190"
     CDS             complement(31986..34295)
                     /locus_tag="PLG69_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPI42153.1"
                     /translation="MEANRKAPAPFLLPVLFWTIGIILAKCFLQGINFQLIIAGGLTI
                     VAIFMRKIRQILLLLLFLFLGMLRFSLCQQESSQLAMLLQEKEHLQQEITFNVTRVFS
                     VEEKRYAIELDSLASYPLQEKIILSTNENLIPGRTYRLLAEIYPLTRDPILDIYPNRY
                     SAVAYQLGKAQEFPNLKVSAIIGRLRYELLSSLEAKLGEDADWAKALLFGESGLKQEY
                     ITQLTGAGIIHLIVVSGMHIWFIYLIMVSLLRMFFKREIAEFLFLPLILLYAALNNWA
                     PSVTRSIIMISVGILARWLQSPVSGAQTLALSLFIITLLSPLQLFNIGLQLSFVSVLV
                     IFYGLPNFRLFSRDKILSNPFYKVLNDLQDGILLSALISIAITPFTLYYFGTASLNGI
                     IGNTLGIPLSGILLPLSFLVLITPQSWYLTKIFVIVYRAVNILWIRWMQFCYNLPFSL
                     SANYLSLFQAMALAVVILWGFFLIRGKFKFALQAVIPMSLLVTGFMLIPTWQKPEASV
                     YIFNCGVSDCTLIRLANGETMLIDTGGTKKKGFAENILNKSDYSEESWLSKNLLPWLG
                     KKGITKIDYLVLTHLHSDHCGGLITLLNHKKIKHIFVSDESTKQELWNYASKQKYFGT
                     AQIHTVTDTCSFFLGKAKLKFLHPDKSYHPLNENNSSLVCRLDTKKERLLFTGDIEAE
                     AENYLVDNYAQELKADFLKIPHHGSRNSSSSEFLQAVNPQEVWITASSRNIYHFPHPE
                     TIKRLQKQQTKILITGNGTIRKMIDSRSN"
     gene            complement(34607..35020)
                     /locus_tag="PLG69_01195"
     CDS             complement(34607..35020)
                     /locus_tag="PLG69_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42154.1"
                     /translation="MRVVDRLEFPKRYKPKQFILIIVIVLVILGIFIQRSGVRRNASR
                     IQISDVRISNFGTQFIELEYTLANTGKRDQEIALMARVWDVEGEEIASALFSVKVKAN
                     SISKRTKMLDKLNRALQEGERPYKVEIALYTRHIP"
     gene            complement(35031..36557)
                     /locus_tag="PLG69_01200"
     CDS             complement(35031..36557)
                     /locus_tag="PLG69_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42155.1"
                     /translation="MAKKICLAILLLFCSLLPALRLIPDSAFAPEIQEGLKPSLLSYP
                     QRAKEDSISIYNNGLYLPFVSRSSVGDAVLDICRLGNTNYSILSYQSPDYKLRGELNF
                     IGGLEPVKKDDSYSFLYRGWQLKSQYGKHIEMNTKWWNGIFLGNQNAARNSWLVDGYC
                     NTTDEKLNIDNLSGDISYNAKNLTLALGRGKFPVGNSISGSIILNDGVNDYGYILAEG
                     RAGIFSISFLHTSLMADSTYNIYNDHFYNNKNYPDKYLALHQFGFHPCSNLHLFLGEE
                     IIYGNRSPEISYFLPNAFWRAIEHNLWDRDNVMIFAGGSYRPVQPLFLYAQVLIDEFS
                     SSKLFSDWWGNKYALQGGMRWNVPFSLPESPSADITTEITAVRPFTYTHYLNHTMFST
                     DGRPLGYPKGSNLVDVSCLLRLPYKDIFLWQGGVSYCKQGSFGSDWRQNYHNVFNTAE
                     EIDEGTADWFQGTKTETITLKSNVLIDIFAHHRLLFGFETSHLDDWQNTFYSAWQFIY
                     "
     gene            complement(36561..37610)
                     /locus_tag="PLG69_01205"
     CDS             complement(36561..37610)
                     /locus_tag="PLG69_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42156.1"
                     /translation="MTKRFLVTLILLTAVFCSALEVTYIDWQNYGSAATRIVIGLYGT
                     GDYKVDKSNDFVEIKLDNADVSKVDISNSAEGLIKSIEQFGDKIVISTFCPYCLEQME
                     LDAPRRLVLDLIVAQPDKQQKIELADFYTSVGKLNSADKIYSELHRNYPSDYEILYKW
                     ACLLKQRGSGRLKEIVGKIPEKSEYYSLAQQMLKGTEKKDSAQLCSPQNAQNACKISS
                     VEEKTTTDSLYIMPKTQQDKKQTTKPAKIKKLYGKKWISGIMLLIILILLLILIYHFI
                     ASISRKKRKTTLNINIEPENKEDIHPEENNTLCLMVRRLLDFGWTNSEIAKELKISEE
                     EVERLVQLCNQDESQ"
BASE COUNT        11928 a         7474 c         6369 g        11883 t
ORIGIN      
        1 aaaataaaca tttatgcttg acacctatgc atttatatat ttactggata aaaataagca
       61 acaaaaaggg agaaaaaatg cttagaggta atgaaataaa acctgcagaa ggcaaacttg
      121 gcgttatgtt agtcggttta ggagctgtaa gtacaacttt tgtagctggc attcaagcaa
      181 ttaaaaaagg catttccgag ccctttggtt cggtcactca gatgtccaca atccgtttag
      241 gtaaaagaac agaaaataga actcctttaa taaaggactt tgttccctta gctaatttgg
      301 aagatttgga atttatggct tgggatattt ttgaatacga tgcttatgaa gctgctttaa
      361 aagcgggagt tctggaaaaa gaactgctgg ataaaataga accggaactg cgttctctga
      421 aaccacaaaa ggcggttttc aacaataact atgtaaaaaa attgcacggc acttatgtga
      481 aaaccggaat caataaaatg gatttagctc agcaactgat ggatgatatc aaacgcttcc
      541 aggaaaaaaa taattgtagc cgcctggtta tggcttgggc tgcctctaca gaggtctatt
      601 tagaatcgga ccctgttcat aatactctgg aaagttttga acagggtttg cgcgataatc
      661 atccggcaat tgcgccttca atgatttatg cttatgccgc tttgcaaatg ggaatccctt
      721 ttctgaacgg agctccgaac ttaaatctgg aaattcccgc tctgagaaaa ctggcggaag
      781 agaaaaaagt tcccgttgca ggtaaagatt tcaagacagg tcaaaccctg atgaaaacta
      841 taatcgctcc aggtttaaaa gccagaatgt taggaattca cggttggttc tctacaaata
      901 ttttaggtaa tagagacggt gaagttcttg atgaccccga aagctttaaa accaaagaag
      961 ttagtaaact ttccgtgctg gaaacgattc tggaaccgga aaaatatcct caactctata
     1021 aagaccttta tcataaagtc cgcatcaatt attatccccc gcgtggcgat aataaagaaa
     1081 gctgggataa tatagatatt tttggctggc tcggttatcc gatgcaaata aaaataaatt
     1141 tcctgtgcag agatagcatt cttgctgctc ctgttttgtt ggatttaatt ctctttatag
     1201 atttggcaca gcgctgtaat atgtccggag ttcaggaatg gctttctttc ttctggaaat
     1261 caccgatgac ggctcccggg ctctatcctg aaaacgatct tttcattcag caaatgaaaa
     1321 tgaaaaatac cctgcgttat atgatgaaag aagaccttat tactcatctg ggtttggagt
     1381 attataacga aatgtaattt tcttgtatgg ggcttgtctc catacctatt ttgatacttt
     1441 tagcagatta aacagacgat gaagaagata aaactcctgt tcagaatagg ttatgcctat
     1501 cataagtccg cctttgatcc gattatagac ctcttgctga ataacgataa atacgatgtc
     1561 tggttctctt tggatatgga aaaaataaag tatttcatct ttgaatttcc ctatcggaac
     1621 cagataattg aggattggaa aaaacttggc taccgtttta ccaacgaaac caagggcttt
     1681 gatatcgtaa tttccggtga cacacttcgt aatgcaaagg actatggaaa aacactattg
     1741 atttttctga atcacggaac aggaattaaa aatatacttt atcgcaattt agctcgctct
     1801 cctggggtta aataccagat ttttgtggaa ggacaacata gagttgattc cttgttaaaa
     1861 tgcccttatt tgggaaaaag cgaagtccat ttaatcggct tacccaaatt ggactattac
     1921 tttcagggta aatttaatag agaagaggtc ttgcaacgct ggggtcttaa tcccgctaaa
     1981 aaaacaatcc tcttcgctcc cacttataaa ccaacctgta tgtatgaaat aaaagatgac
     2041 atctttgagc aaaccaggga ctataactta ataattaaac ttcaccctta cagctggatg
     2101 ggaaaatatg ccccgcataa acaacatcgc atttttgaaa aaagggtgaa aaagtatccc
     2161 cattccgtgc ttttaccttt tacagaattt aatattgtcc cttattatgc tgctgccgat
     2221 acagttatca gtgaggcatc cagcacggtt tttgatttta ttgctctgaa taaatttggt
     2281 attgtttatg accttgcttg cgataaactt aatcattcgg acggtcaacc tctgttggaa
     2341 attgataacc gggagttttt aaaaggcgct tatccccata tccaaaacgg aaaacagctt
     2401 cctgaagcaa ttgctacagc tctcaatcct actttagata tgattgccaa agcggatgaa
     2461 taccgtcaac gctatttcta tggcttggat ggaaaagccg ctctccgttt tgtagaaaaa
     2521 atggaagaat tatactatga aggcggtcac gaaaatggag tgtaaatttt gaaaggtgta
     2581 ataatttctg ccggaattgg cagtcgcctt tggaaaacta caaagcaagt tcctaaaacc
     2641 ctgcttccct ataaaaacgg aactattctt tccactatta ttgaacagct aaaacaggca
     2701 ggaataacgg aactgattat agtagttggt tttaagaaga attatatcgt agattatctg
     2761 cagaaaaatc ccccggcttt accagttact tttgtggaaa accttgaatg ggaaaaaggc
     2821 aatgctcttt cggtttataa agtaaagaat tatataatag ctgaaccttt tctcctttct
     2881 atggctgatc acctggtaaa agtggaggca ctgaaacaaa ttgtggattc ccctgaacgc
     2941 ataaacctgc ttttagttga cccctttata acggaaaact ttgatcttga tgacgctacc
     3001 aaagtaaaaa ctgaaaatga ttacataatt gccatcggaa aagaactgac tgattacaat
     3061 gctattgatt gcggaatttt ccgtttggaa ccggatttct tttctgcggt agaaaaggca
     3121 gttgccaaag gaaaggactc tatcagcaat gccattgcag aattgatacc gttaaagcgc
     3181 ataaagtcaa ttactttaaa taaagcaaat caatggttgg atatagacac tcctgaagca
     3241 tatcaatttg ctcaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncttat
     3361 tacataagac gcagccatca atcccattac agcaggtaaa taactgatag aaccaataac
     3421 ttgtttttgc tttgctctat caataactcc ctcttccgaa atatcattat ttttttcagg
     3481 aacaaggggc aactcggaag aataaacaca gggaaaattt ccctgaatac ctctcttgct
     3541 taatagtttt cttactcttc ttgctaaagg gcaattgtag cttttatcaa tggtagaaag
     3601 atgaacttga aaaaggtcta aacgatttcc cgctcccatc acactgataa atttgccttc
     3661 atttttaatc aaatactcca atagacatat tttggaaccc aaactatcaa tggcatctac
     3721 acaataatcc gctcccgcaa gtaatctttc ctgattttcc ttatctaaaa attccttata
     3781 aataatcaca tctgcctgtg gattgatatc cagaatcctc tgctgcatta tttccacctt
     3841 gggcttgcca attgtggaat gcaaagctaa aatctgcctg ttgatattag ttgcctcaat
     3901 tacatcaaag tcaatcaaga taaatttccc tattccagaa cgagctaagg cctctgcaga
     3961 ataagaacca actccaccca aaccaataat tgcaacgcag gttcgggcta atttctgcat
     4021 tccttgttgt ccaactaata gctctgttct ataaaaattc gctttattca tagctgttaa
     4081 agtttttttg ttgttgttct attaatttac ctcttggaat ttctgcaaat tcagctacag
     4141 aagaaataag agtatttaag cgcaaaagag gattagtttc ttcttcctgc acataatctc
     4201 gggtaatatc tgtctcaaaa agatagcgtt tactatctat catagctctt aaaagctctt
     4261 ttttatccca cttcaataaa cgctcggaaa tagtaaaaaa ggactttagg cgtaaaagtt
     4321 ccttaaaacc ctgtatggaa cctgcataac catgcacaag ataggacaga ttatattgtt
     4381 tcagcagctt atatgcttcg ctaatatgcc caaccagatg taaaaccaca ggcaaattgt
     4441 attgtttggc aagttccagc tgctctataa atatcttcag ttgacctgca aaatcaggat
     4501 tgtttctgtc caagccgatt tctccaattg cccaaatttt tttctctatg caaagttctt
     4561 caatatcctt cagttccaaa tcgcactctt tgtaatatgg atgaattcca gcactatagg
     4621 caatgcgttt atcagggttt ttttgatgcc aggcaatttc cgattttgta agtgcagaag
     4681 aaagccaaaa gtccaagcca aatttctttg tttcatccat taagggttta accggcatta
     4741 gcacatccaa attggctaaa tgacaatgcg catctattat aaaagccatc gttctttcct
     4801 atctcaaggt agtttctact gccttaaaaa ccggctctac ttctatctgt ttcatacagt
     4861 tatgatgttt ttgcggacat tttttactgc catgactacc acagggtcta cattccagtt
     4921 ccacttcaaa aaccctgtct ccttcccgat aaggataata gccaaaacgc tgaaccgtag
     4981 gtccaaaaaa ggcaaaaacc cttgtctgca tagcattggc aatatgcatt gcgccactat
     5041 cattacaaat cattaaagaa cattttgcaa ttacagcagc actttgcaat aaattagtaa
     5101 ctcctgctag atttattaga ttagtattat ttttccccaa ggcattttca atttcattac
     5161 ttatgagttt atcgctttcc cctccaatta aaacaatagc ataaccacta tctaaaagtt
     5221 tctggcagag tgagatataa gaattcggtt cccagcattt ggtttgccag atagaacccg
     5281 gtgctacagc tattattgtt tttccggata agggttgaag taatttttcc gctttctgcc
     5341 tatctttttc ggaaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgtc
     5461 ccacttcttt ttgaaagccg gtgaacgcaa tatgtccctg caaacctaat tcttgagcaa
     5521 tatttttcat ttcggaaagt aaatctcctc caccaacagc cataaaaaac aggtttggat
     5581 tttcttgcac tgccagagag gcagctttca gaaaattggg gtagtccttg tgacctgcaa
     5641 aagcagctac agttccaaca ataatgcttt tttcaggtat attccaggtt tggcgaaaat
     5701 ctgtcggaac ctgaacagta tcaaatttat gcaggtctat tccactataa ataacaacaa
     5761 ccttttcaga aggaacacca caggaaatta gaactatgcg aatattatcc gaaacagcaa
     5821 caattttatt aactgctttg gttttatact taaaagcgga taaaggattt ttacctatag
     5881 gaaagtcaac tcttctggtt gctacaagtt ttaagctcgg ataaaagaac tttgcccaaa
     5941 gaccccaact gagagaatga ctgctatgca gttgtaaaat attataagaa taacgacgac
     6001 aaagacgagc taaattaaaa gctgaaatta tatctgattc acctctgaac ataagacaat
     6061 gataaggtaa ccgttttttt tccaaatact gctgtaattg agaaccagga caacaaacaa
     6121 aaccacattt ataatcactt ttaagcatac cttcataaag atatactgcc tgttgttgac
     6181 cccctctcca atgtttttcc gcatcaatat gaagaacttt cattttttca ttttccctaa
     6241 aagatgataa cgataccaaa cgccccaagc agaattttta cacagtaaaa aacccttggc
     6301 accatccaga aaacccagct ggaaaatgaa cattttaagg aacttgaaga tgcctctaac
     6361 ccaggcaata aattcattag ctcttttgcc ttgcgcataa agctgtttgg tacttaattc
     6421 tccatacaaa cgcattttag tataatgaac ttctgctgta ggattagtaa aatgataaag
     6481 ataatgctta caggacttaa ctttttgcga ggtctgcact ccttcatgaa ccagtttttc
     6541 gttaaatttt ccctgctttc tgttaaatac acgcaatgga gcatcatttt gccaaccgca
     6601 aaaatgaatt ggtttaccca aataataaga ccttcttttt agccgccagg cagtgttctc
     6661 aaatctttta tttttcagct cctgcagttc tttttgcagt tccgagctaa tttcttcatc
     6721 agcatcaata gacaaaatcc agtcaaaagt agcaaggtct actgcttgct gtttggcttt
     6781 gccaaaacct tcccatttat ccaaataata tattttagct cccaattgtt tacagatatt
     6841 cactgttgca tcgctggaac cggtatccaa aatcactatt tcatccaccc aagaaataga
     6901 ttttaaacag cgggcaatat tttgttcctc gttttttaca atcaaaactg cagaaagttg
     6961 ctccatttac cggtttctgc cttcttcaac tactttggtg tattcaactt taaaacggtc
     7021 ataattaaaa tgctcttgca catattcccg agctgaatta ctcatctgct gtaataattt
     7081 cttgcttgaa ttcaatacct taatatcaga gataaattca tctattattt cctgttcttc
     7141 tttggattta attaggaaac catttattcc atcttgaaag gattctctca ctcctcctac
     7201 atcagtagcc aaaggaacca gaccacatgt catcgcttca gctattacta aaccaaaacc
     7261 ttcggaaata gaacatagaa ttaaaatatg aaaatccttt attgtctcat acaacttctg
     7321 acggtcacaa atttccccta aatttattac gggctcggta ttaatatggt cgccaatgca
     7381 gtaaaattct attttcggaa attcccgtat aattttacga gctacctgct gatataagta
     7441 aaatcttttc tcttttgcct ggcgcgcaat aaaaacaatt tttaggggtg cttgataatc
     7501 tttaggggaa atatcatcgc agagaggaat agaattgtag attaatttta agcgactatt
     7561 atatttttcc tcccatccat tagctttata atactgttgc acttcatcat atactgcctg
     7621 agagacaaca atacgtttgt taattttggg agtagctaaa agccagattt taggatttat
     7681 gccgtagaaa ccatgcaaaa tattaattaa ataactctta gtaagatagt gagataattc
     7741 gtaaaatata gtgctgttgg cagaaataac tactgattta ggatgtctgt ttatcattga
     7801 agccagaaat ccatagaaga aattcttaac tataaaacga tttgtgttat taaggtattt
     7861 gtatatttca atcaatcttc cgcttttttg cattgcctga taatgggttt tgttacgcga
     7921 attcttagta attataataa aaggtttttg gtctgcaaaa atacgcaaaa tgtcaagatg
     7981 aacctgttct gctcctccag tatgaaaatg aggaaagaaa aaaaagaggg aactgggatt
     8041 attccaatgt aaaatcttat cggctaaaac ccccgtataa tagaaaggat aacctaatac
     8101 tcgctctaag ctattcattc taatatctct aaatcattac gaatagaact taatcctttt
     8161 tggcacctat aatttctatg tgaaatcttt tttttacagc gttcgctata attggcattt
     8221 tttctttcac cctgatcgtg ataatgtgta tgatacaggt gaagagggat ttggttagac
     8281 acaaaattaa aaccgctttt tcctaattcc tgtaagcgtc tacccaaatc atcatcctca
     8341 ttcccccaac caatatagtt ttcatcatat ccattaacca attcaaaatc acttttaaag
     8401 atagcagata cacctcctcg caattttgcc ccgtgtccac caagatgtag ataacgaaaa
     8461 agcaaatagg atagaaagtc ctttttatac tgtttttgta tttttctctt ttgttcagca
     8521 gttaaaattg tattccagtt attctgttct ataatttcaa aagttaattt atcgctttgt
     8581 tctttactca aacgcacagg atagccgctt aaaaaccttc tccggttaag atttgccatt
     8641 atacatttta aataatcttg cggaatcaca atatcctgat ccagaaaaac caaaacatcg
     8701 cctttactaa cggaaacacc gttgtttctt actctggcag cgcgaaattg aagatgcttc
     8761 tgtctcacta aaataagcgg aattgcggtt ttcctttttt gagcgttata aaatgcaaca
     8821 atatcttcat ccgaaccatc atcggaaagg ataatttcat ccggatgcac actttgtcta
     8881 tacacagaaa acaggcattt ttccagtaag ttcaaataat taaatacagg aacaattaag
     8941 cttatactca tcgtttcaat aaaatatagt ttttgaaagt gccaatctgt ttaccgccat
     9001 taaaatgttt ttctataaag gttaaaaagc ggtatttaaa ggtttcgtgc ttatgtggtt
     9061 cacgataagg attgggtttg cctgtaagtt gcaatttatc ctgccagtca aaacaggaaa
     9121 tcctttcctg cataacttta ggatgagttc cgttaaatac tgccaatagc gacaaaggtc
     9181 cataatcaaa atattgaggt gcattattat aataagcatc tgctttttct ttcccccagt
     9241 gcacagaatc caaagcccgg cgtttgtttt gcattaattg aggagggcgt acccaaccgt
     9301 agtgataaat ttctgcatca actttagcaa ctttcagttt acgggtacct ttttcctgtc
     9361 tgggattatc gtaatattca aaatagcgaa aggattgagc actctgataa gaatgaatat
     9421 gtgggtcgtt acggataatt cttatttcaa aaggatacca gccatgccca ttttgatagt
     9481 ggtaataatc accccaaaaa tgtttatagt aaaagagtaa accttccact tctttatcgt
     9541 ttaacagttc ttcacagcgt tttttaatca ccggtaaata cttttcgtga acgacttcat
     9601 ccgcttgtag atagaaaagc cagtcacctt gacattgatt tttggcaatg tcagtttgcc
     9661 aggcattaat tactcccctt ttacacaatt ttgggtccca ttctgtatca ataattttta
     9721 ttttcggttc ctttaatgcc aaaatgcgtt ctttagtatc atcgtcaggg tcactcttac
     9781 ctattgcgat tacgaattca tcgcaaatag gcaaaatgct caatatggac tccaccaccg
     9841 ggtagtatag ttttattccg ttgcgtacaa atgaaaatcc acttatcttc ataattgatt
     9901 ttcccctttc tgcctttttt gtaacttact taccctgttg taaattatag agatgctgat
     9961 agcgaggaca attttgcatt aattcctgat gtgtgcccat acctacaatt tttccctttt
    10021 ccaaaactac aattttatcc gccttaagaa ttgtagaaag gcgatgagca attaggataa
    10081 cggttctgtt ttgagttgcc ttatcaattg ccatttgcac tttttgttcg gaatctgtat
    10141 caagtgcaga agttgcctca tcaaaaatta aaataggagg gtctccaaca atagcacgcg
    10201 cgatgcaaag tcgttgtttt tgtcctccgg aaaggtcaga acctttgctg ccgagaactt
    10261 cgtcatattt attcggcatc tcatttataa attcttccgc attagcaatt tgggcggctt
    10321 taatgatttg ttcgtcgcta acctcttcct ggcttccgta agcaatattt tccttgatgg
    10381 atttggaaaa gagaatggaa tcctgagtta caacaccgaa aaacttgcgt aagttatcca
    10441 atttgatatc tcttatatct attccatcta ttttaatggc tccgtaagta acatcataga
    10501 ggcggttcat taagttggca atagttgttt ttccaccacc actggcaccg acaaaggcaa
    10561 ctttacttcc tttaggaata gttaaactaa aattcttgag caccggtttg ttttctttat
    10621 agccaaaagt tacattttca aaagttattt cgctatcaaa ggtttcttta gtaatggcat
    10681 caggtttatc ttgaatgcgg gattcttggt tcattaccaa gccaattcta tccagcgaaa
    10741 caagagcttt cctaatatca gtatagacct gagtaatcac ttttagcgga tgcagcatag
    10801 aaaacagagc aaaaaggaaa gctgtaaaat cacccaaaga aaaactgctg cccggggtaa
    10861 gaatcatttt cccgccaata atgattacta ttatgccggt aattgcagta tttaattccg
    10921 aaatgggagt gctcaaagtt ccgtaatatt gtgccttaaa ccacatatga taatgtttat
    10981 tgttgattgc ttcaaaattc tttctctcat attgttcgtg tctgaatgcc tgcacaattt
    11041 tcatactatt cagcacttct tccacattag agaagagatt ggaaagctgt tcctgaatgc
    11101 gtttggaata tttttttatc ttcttaccta aaagacctat cgttaaagaa aataccggta
    11161 aaataataat gctgtataaa agcaaaacag gattcaataa atacgctatt cgggcaaaaa
    11221 caagcaccgt aatcatttcc cgaattacat tcacaaagga attgattagc tgttcactaa
    11281 cgatatctac atcacctacc attcggacaa tagcatcccc aatgcggtga cgactgaaaa
    11341 aagttaagga ctgatttaag taacgggaaa acatataatt gcgtatatcg cgaatcgttc
    11401 ttcctcttac ggaattgaaa aatatcttgt gtaaaagata aaagacattc ttgaaaagga
    11461 taactaaaaa aaccgctata cataataaat acaataaaga aagggaatcc gtttgcagca
    11521 tcagcacttt agcatcttcc cataaggcac tataattttg aattgccggg aaaaaaccgc
    11581 cggaagaact gatatgctgt tttagcatct ctcctaaagc tacaagaaaa ctgctgaaat
    11641 cggtgtataa caaattgatt ttattcggct tgaacacata atcaaacagg ggaataacta
    11701 aagttacgct cactccatta aaaagagcaa aaagcaacat acttatgaaa ccagcaagca
    11761 tataatgcca gtaacgcaac atcatcctga aaatcaggat aatattattc catctgttct
    11821 tgatttttcg tttcattgcc ataattaatc cacaaaaaaa agccagcaac tgctggcaag
    11881 aaaaaagtgg cacgccctaa gggagtcgaa cccccaacct tctgatccgt agtcagatgc
    11941 tctatccaat tgagctaagg gcgcacatta atgtcataaa attttgtctt gttaatctgt
    12001 caacttattc ttgcaaaaga acctcactcc gttttatagt atttaagcag taattgatac
    12061 ctcatatttt gcctaaaata gaacaaaaca cgaagatagg attagcattt cttttttctc
    12121 tctctcgcta ttggttatta catctatttc agcaaaacga ccaagttctt ttcttttctc
    12181 ctgaagaaat tataagcatc ttcggcaatt taccaaatat ttctcttcca atgcttattt
    12241 cttgctttaa cctgttatga ccaggtaaca tttatgtaac aactccgtaa cgaaactact
    12301 gcagtgttag ggaagtgtta cttggtcgtt agagaattga ctatgtagca agcaattagg
    12361 caattcaaat aatgccccgt gcaataggga aattttttat ctcctaaaaa cactgtaatt
    12421 atctaaatca cagagagtta tttatagctt tccggtgctt ttcattcatc attcttcatt
    12481 tttcgttcta tccgataaac agttttacga ggatagctat caggagtatg gaattgattg
    12541 ccagccaaat ttttattttg ttaaagcttt tgatatatat ttcccggttt tgctgaacct
    12601 ttttttccag acgaaccatt aaatgttcca gggaaccaat tttttcagct gtccgatctt
    12661 tttttctgat attttgatcg tgtttggcat ttcgcttata aatatggatc aattcataag
    12721 caaaagggat aaaagtagga attaatttct tgaatttcat actaaccctc atttgaattt
    12781 atagcccaaa gaaaaataat gtgttccggg caaaacacta tgataggtaa atgaataatc
    12841 cacttccaaa ttctggatat agatgcttaa accggcatta taggtagcgg gattttgatg
    12901 gacaccgaag cgaattgcca agggttcaaa taaatgagct tccactccac ccataaattc
    12961 cgtttctttg gcaaagtcct ttttcacttc tatagaggta gtaactttct cataaggaac
    13021 ataagcaatt cctaaagcca gtttactggg caggtctatt tgattttcat cacccattgt
    13081 ggctttatta atattggtaa cggcaaaggc aatatgggtt ctggaatgca aaacagctaa
    13141 agctcccagg tctattccca aggcagtatc gtcatcttca ccatccatac ttaagaggta
    13201 caaatttcct gtataaccga aagaaattcg ggaatggata tcctccagta aaatgaaact
    13261 atgtgccaaa ctatacacct gctcactcat caggttaaca tcttcaaaat ccacatccat
    13321 tgctcttgca ccaatagcaa ccgttcccag atttttagga agtttataac ccaaagcaac
    13381 tgtcttcaac tcactgaagt cctgattgta aagctgggaa aagctaagtt tagcgctaaa
    13441 agtagtatta atcagcccgg caggattgaa aaatatggca ttggcatctt tagaaacagc
    13501 tgtataggca ccacccattc ctcttgctat agcagatggt tgatagtcat caaacacagc
    13561 taaaagtaat acggatggga ttagaagcag acagacaaac aataattttt tcattataaa
    13621 cctcacttca aagcactttt aattacaatg ggctgaactg tattatcttt tcccccgcta
    13681 ctccgatcgg taacttccaa atgacaataa tacaaacccg gaggcactaa ttttaaattg
    13741 gcatcgcgtc cattccattt aaattcctga atacctgtgg cagaagttat atttgtatgc
    13801 acaggtgtat aaaccaaacg accttgagca tcataaattc ttataactgc tttggaaaga
    13861 taaccggtac aggttccgta gctgataata atttcttccc ccatttccgg attgaagata
    13921 tttttcttgt ccccttgttt accaatattc agataggcaa ttttcttcgg tactttaaca
    13981 attacagagc catcaatcag gttattaact tctgtctcat tatatttgaa agagacaagg
    14041 tcaattatgt tttctccata aacaagaggc ctaaccagaa ttttaattaa caaagcatca
    14101 ttatcaggat aaataattcc gtcctgagaa aaataagtcc agctgatagt atctccaccg
    14161 gcactaattt gaaaatcagg catttcacga gttaaagtgc tgtcaatatc caaccccgaa
    14221 aaaataagtt tggtgggatt aactttgaaa gtaacggaat aactcatcac tccccattcg
    14281 gacttaatct tggtgctaat taaattcata gaaacatcag aattaagggt gggctcagtt
    14341 gtaggatcgg ttcttactat ttttataatg gaattctcta tagtatcctg cttaatcata
    14401 tcgctggcat tacgcggatt tagtttaaaa cctgcgctac tatgataatc aacaatgccc
    14461 tgtataatat accaactatc acctacaaca attgaacttg ccggaatggc atataatcca
    14521 ttatctacca tagcccaaat attactgtga tccgctatta aaaattgacc atagctatcg
    14581 ggagtggact taattgtaac atcctgaaat ctgaccagca ctccttcata cttctcattt
    14641 tcaggatgat tagttccgga aggcatatct gctgtagata attctaagaa aggaggcaaa
    14701 ggattacctt gagatattat ttcataagca cttacattgg taaattcggt taagttataa
    14761 tattcatcaa ctgttccggt aagctttacc agatcaccta tgcttacaac attactggtg
    14821 tttctatgat aaacataaag tccgctccat tcaccccctt ctgggtctgc aatataaaaa
    14881 gcagaattgg gtataatatg agttacgatt ccttgaacag taactgtttg ggtgtaataa
    14941 ggcgaattac ctgtaggatc ggtagtatat tgaatgtcgt gacaggaaag aactgtttga
    15001 gcagatattc ctgccaccag gaaaattgcc agaaaggtaa gaatataatt cttcatttat
    15061 agctccttat taaattcact gatttctata tgttctcctg tttcgggaag agggctcggc
    15121 ttatctttga taaacagcat atcaatgatc atcaaaacca ccccgataaa gattgaacta
    15181 tcagctatgt taaacacggg aaaacgttcc ataataaaat cgggaaaatc catagaaaaa
    15241 aagtccgtta ccggtccaaa taaaacccgg tctatcaaat ttcccaaagc tccacccaaa
    15301 atcaaaccga aagcgataac ttgaatcttg tgttgagcat aatgtaataa ataaataata
    15361 atgcctacta ccagaaccgt tacacagata aaaataatac ggttgactaa gtcactacca
    15421 aaaccaaaag aaaaagcagc accgctattt tgcactctaa ttaaaataaa tgtatcacca
    15481 aataaatttt gcaacaaagg aatgcattca tacatttgca tattcactcg caccaaggtt
    15541 ttggttaact gatccaaaac taaaatcagc agcataataa aataagccgg agccgttttt
    15601 aactgtgtac gaggttgttt caacgctttt gccgcctttt tttgtcttcg tttttctctt
    15661 tacaggcaac acagcaggtg gcataaggaa taatttccaa acggttttcc tgaataaatt
    15721 caccgcaaaa ttcacaaata ccgtaggttc catcacttat tctttgcagt gcttcattca
    15781 gctgacgaat tttttctctc tcgctttcca gcatcataac cgactgctcc ataaggttag
    15841 tatccgaacc ttgatctgct tgatgaaaag catagctgga aagatcaccg ctactttcac
    15901 gagcacccaa actttgttct ttattgatgc tctcaatata agcaatactt tcctctaatt
    15961 cagcccgaat aacttcttcg tagtgtttca gtttttcagg cggtaacttc ttcgttgcca
    16021 ttatattctc cttcggtttt gctcttttaa aaatcaaatt attcaaatat tataatacca
    16081 attatgatgt caatgaaaaa gtccttgtta accttaaaat caattctgct attagctttt
    16141 gtagctgctt tcccgatata tcggcaattc tttgaatttc ttcttgacta tgtgccaaat
    16201 tatgaaacag attactatag ttagtcacaa ttgaataggc aagaacttta agccctgaat
    16261 ggcgagctgt tataacttct ggaactgtag acattccaac aaggtctgca ccccaagaag
    16321 caaaagcaga gcattcggct tttgtttcca aagaaggtcc tgtaactgct atataaacac
    16381 ctttagctgt tttaattccc aaatcaaaag ctactttatc tgccagcaaa caaagttccg
    16441 gatcatatac cttattcata gagggaaatc tttcacccaa atcatcatca ttcattccta
    16501 ttaagggatt agtgcccata aagttaatat gatcctgcag ttggacaata gttcctggtt
    16561 ccagttcttt tcttaaactt ccggaagcgt tagtaacaat taaaaccttt attcctaatg
    16621 ccgataaaac ccttaccggg aaaacaactt cctgcattga atagccctca taataatgaa
    16681 agcgtccctg taaaaaaata acaggaattc cctcaacttc acataacagc aaatttcctt
    16741 tatgcgtagg agcagtgctt tgaacaaaac cgggtatttg attataaggt atagaataaa
    16801 gtagcggaaa tacttctgcc atattactta atcccgttcc taaaataact gcaatttgtg
    16861 gttgtttagg taatttggtt ttcagccagg cagaggtatc tttatagctg ctcatttatt
    16921 tgttcttctc gttttgtgtt tccgtatttt ccggtttggg cacaatttct tcttttattt
    16981 gatgaaattc actatctaac ctcaattcac tgtctttgct taaaagaggg tctttattta
    17041 ttctatcctg aaaactttgg agttccacct taaactgcat caggaattgt cttttctgtt
    17101 cttttaattg gagatattga tgttccagca cgcttaaata ttgctttgct tcctggatag
    17161 ctcttttagc tttcaggtct gcttccttaa taatattttc tgcttccttg cgagcgttgg
    17221 ctataatatc cgctttggtt ttttctgcaa aaacaagagt tcggctaatc aattcctctc
    17281 ttcttttcaa ttcttctact gcactaccgg cttgtaatgc ttcttgtttc acttcatttt
    17341 gtttttgttc ctgacgggca atttcttttt ccagctgttt ttgaaatgct tcaacctcag
    17401 aagctatctg gtctaaaaaa gcacgcactt cttttttgct gtagccaaac attgcagtgg
    17461 aaaactcctg atgtcttata tccaaaggaa ataggggcat aaattcctcc accttaaatt
    17521 aacgaagtta aaagacgaga taatagatca agaataaaaa atgcaattat cggagataaa
    17581 tccaatccca tcatcggagg taaaatatga cggattggtc ctaaaaccgg ttctgtgata
    17641 cttattaaaa aatgatataa acgattatac ggattccgaa ttatccagga agcaaaaacg
    17701 cgcgctataa ttaaaatatt atagatctga ataatccgaa ctgcaaaaag taatatggaa
    17761 cccgttaaac tggttgaata tatcaatcca aaacctcatg acaatttctg catcttgctt
    17821 cataagcatc tgtggaacca accagaatct gctcatcctt atgaattgtg cgttgagttc
    17881 tattagcagg attaccgcag accacacaaa tagccaaatt cttggtaata tattctgcaa
    17941 ttgccattaa ttgaggcata cttccaaagg gctcaccttt ataatcctga tccaaaccgg
    18001 ctacaataac cctataacct ctatctgcca aatcattaca gataccaaca agggaaggat
    18061 caaaaaattg tgcttcatca atagctatta tttttacatc gtcatccaga tagctgtaaa
    18121 tttctccggg agcatttatc ggaattgaag gtgcctgcat ttgtgaatga gaaacaatat
    18181 tattggcatc gtaacgatta tcaataaccg gcttaaaaac cagcactttt tgtttagcat
    18241 attcagcgcg tctgattctc cgaattagtt cctccgtttt accactgaac atacttccgc
    18301 aaataacttc tatccatccc gttttctgat ttacaatatt caacattcct gttcctccct
    18361 tagaaaatat attttaatta ccaagtcctg tatctcccct tttttgtcaa ttcttcattt
    18421 accgcatccc tttcgtaact tccattatag cagaggaaaa ctattataga acaaggatat
    18481 tttatttata gaccccgcta aaaaatgtaa ttatattttc ctaaacttac ctgaatactt
    18541 acctgatcag taattataaa gcgctttact taataaagga atgatattat ccaggtaaca
    18601 aaaaacggac ttcctcctcc catatagttt ccctatatct gaaaactata agaaaatacg
    18661 cgtaaacacc acttcccaca aaaaaaactt tttgccggcg tagcttacaa atcaaagtcc
    18721 cgttgtctta tttgtttatc actaagcttt aattactttt aaatatcctt caggcaaaca
    18781 taaagttacc gttcccactt catttcctac agttcaaagt tctgtttcct ttcctgaaaa
    18841 tatcatccta actgaaaaca gaaacaaaat aaaagagaac tattctctaa aaacaacact
    18901 acagtatcaa tttcctgtta ctatggtttg gtatttcatc agaaagagaa gccaggtagt
    18961 gttataatca acagtagaaa tattagttat ccctccattt tcagctgcct tggcaatagc
    19021 ggcatctgag ttcgcaacag gttgaaagaa caaaatacca atttgagtat aaacaccttg
    19081 tttttcgccc aacggattag aagtagcgca aatcggtttg ttgattgtgc aggaactgat
    19141 aattaccagt aacagcacca ctgcgagaag ttttggtagt tgtttcattt ttgtttctcc
    19201 tttatattaa gacacctgta cgggtcttat atttataatg tgcttgatag gaatttaaat
    19261 cttccgcttt tttttgaaac aaaaagccag gtttcatagt aatagatgac agttctcttc
    19321 aatagtattt taattttgaa gcacaaccct gaataatgta attcgggtaa acaaagtatc
    19381 ctttcagcca cgcaattttc catactcaca ctccttttat gatatagttc tattataaat
    19441 ataattataa atttgtcaag tgcaaatttt tttcttctac tttggaataa aataagatta
    19501 gttcaagcta agagtataag ctaaaatacc ggctgaaatt gatacattca gcgattccat
    19561 ttctccgggc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttgggca
    19681 aagaaaacaa acctgctatt ttttgcggct gttccgaatc gcaaagtcgt ttcattcctt
    19741 gttcggaaac ggtataactt attgctgccg aaacggttgg ttcaccttca acgagatata
    19801 gttccagggg tttaatgctc caagcaaaga gctgttccaa agttcttttc ccttcaacaa
    19861 ttaccagacc ttcctgcaaa cggaatttct tctgtttcag tttagaaaga tagcgcagat
    19921 gttcattact cattttagga acggcaacat tttctctcat caactatcct taattccaag
    19981 ttctcttatt tttaatcaga tagggactta tccacaagct ctgtggataa ctataattta
    20041 ttaactgcct gaaaataata gctgttatat tttgtagatg actttccaca aaacttatcc
    20101 acaggttata cacattattc ccaaacgcat aattttagta taagatagtc agcttttttg
    20161 taaatttcct gtttcccagg tgcaatctta aaatgtaaac tccggaaggc agagctctaa
    20221 atttcggttc tgcagaaagc aggaaattgc cttcacccgc aattacttct cccaaatatc
    20281 tatttattac tttttggcct ttcaggttat aaatttccag ttgaatttta cctgcaagct
    20341 cttcacttgc ccaccacctt aaaacggcta aggaagttcc agaaacagga ttcgggttaa
    20401 ttatgattga tgcctggggg ttagaatagg tataatttcc aattaagggt tggcttttgt
    20461 tgccactttg gtcaatagct cggatttgat aagttccggc ttgggagatt tgtagcataa
    20521 ttgtaaacac agcattatcg ggactggtat aaaaatattg aaaatgttca cctccagcat
    20581 ttgttatttc atagtaaagg gaatcgggat cgggatttgc ctgcagagaa atttgcaaag
    20641 tgttataaat cagattttgg gagatgagaa taggtgtttc aggaggaaaa gtatcatttc
    20701 cgatagccga atttacaaaa ctgaaccaac gggagcgaga aaaattcagc ttatgatatt
    20761 gtctccagcc gttttcaaaa ttgaatctgc tatcaggaaa ccaaattgcc gcagttccaa
    20821 tatttcctgt ttcttcagga ttggtaaaac aactgctgat aacaatggaa gtccaaagag
    20881 agagcaaatt ttgtgttccg cttgtccaaa ccgtttgcgc cttttccaaa aaatctctga
    20941 tatctatatc tgcaagtcct gtgttcatag aatagcagga attgcgaaca gtaaaatatt
    21001 taggggcaag aaatctttgg ctgatagcaa aattctttat ctgttgtaca aactgtggca
    21061 ctaattctgt tttcaccact gaacaggtaa cagaccaaag agctcctccg ggatttatac
    21121 cttgtcccgg caaataggaa gaaacataga gttcgggaat ttgcgctaac aactcatcaa
    21181 agtcaccatc aaaaagggga ataatatctt cataaggaaa accgtattgg ggaacaaggt
    21241 cttccgaacc gattatataa tccgcaaagc tgtgaatttc attgattact tcaatgcatt
    21301 gcatactgca agcatcaaac agtaaaatat ctaaatgggg aattcctgaa agtgccaaaa
    21361 ccagttctcc cccggaaaca ctaatcaggt cttgagcatt atcatcggga caaatccatt
    21421 ttccgctgtt gtctttatac cagctatccc catgtgacca aataataagc attttgcgct
    21481 gagcgggata gcggttgaag ccccatttta taaaactatt taaggtatta ggatctccgc
    21541 tattgattgt tcccaggttt gctaaaaccg gtgaagttat ttgttccgaa ttatcctgtt
    21601 gaattttata gcgttttgct ccttccggaa agtcagcttg cacaattaaa ttcaaattcg
    21661 caggttgggc aacactttcc agagaattta tatctaatct gcccatttct gccaaattat
    21721 tatcagcagc catataaaga aggacagtcc aactttctgc actaagaaaa atgccaatag
    21781 taagcagaaa gataatcagg aaatatttca ataaggatat tcctttgcct cttcggttcc
    21841 atttagaact ctaattccac cctggcagag agcttccatt tctttttcgc caggatataa
    21901 atatatcgga gctataaact ttgtatgctc tttaataaaa tttactatat aatcgttata
    21961 tgccaaacca ccgctcagat aaattccatc cacttttccc gcaagcactg ctgaacagga
    22021 accgatttcc ttggaaattc tatagcacat agcatctaaa acaagtttag ctttttggtc
    22081 accttctgca actcttttgc caacctcaat tccgctgtcg gttcctaaat aagccataag
    22141 acctgcaccc ttggaaagat aagtggtaag ctctttttta gtgtatttac ccgaatagca
    22201 catttctatc aaatcgccga tgggaagaga gccagctctt tgaggagaaa agggtcccgt
    22261 acctaaaaga gcattattaa catctactac tcttccgttt tttatagcgg caacagaaat
    22321 tcctcctccc atgtgcactc caatgaaatt aacttcagtt aagttctttc ccagtttttc
    22381 tgccagtaaa cgacaaatat agcggatatt taaggcatga aacaaacttt tatgttcaat
    22441 ttcaggaatg cctgctaagc gcgcaatatc ctccatttca tccgtggcta caggatcaac
    22501 tataaaatag ggaatgccaa gtggttcggc aaccgctttt gccaaaaagg cacctaaatt
    22561 agaagcatga cttcttcccc aaattgtaat atctttcaga tcgcgtaaca ttgcttcatt
    22621 tactttccat gttcctccac ttacaggacg aactaaacca cctctaccaa ctacggcatt
    22681 taaactttgg ggatggacat tattttcttc catcgcttcc ataaccaatg ccttacgaaa
    22741 atccgtctga ttaaaaagat ctccaaattg atttaattct tcaggatcgt ggcgtaaggt
    22801 cttttcaaaa acgggagtgc tgtcatcata aacagcaatt ttggttgatg ttgaaccggg
    22861 atttatcgcg agcacacgat aattcatttt tcctccagaa tgttaatttt gttttgttta
    22921 tcttaaaaaa tacaaggctt aaaaccgtca atcaattttc tttcccttcc cctgctttct
    22981 atatctgctt tacttaaaaa caaagatttg gcttgccaaa aaatatgctt tccaaattat
    23041 gactaaaata taaacaagga tactccaatg accccaaaag aaaaaaacga aacccaacct
    23101 gcagatacac atatctgtga cgaagaatgt cagcatgagc aatttgttat tcccgtccaa
    23161 agcgaagatg gctctactca aaattacatc cctatcgggc atattgacta tgaagataaa
    23221 caatatattg ccttagtacc tgaaggagaa gatacttatg acatctatca aatggtcgga
    23281 gaaggagata ttgttacttt ttaccccatt gaagatgagg aattgtatga tcttatagcc
    23341 gataagttta atgagctttt cttattcgaa atggacaaaa tgggattgct ggacgaggaa
    23401 aattttttag accctgagga agattaatta ttaaccaata atttctctcc ctattcccaa
    23461 aattcccgta agagagtttc ttcgcctcta tacgataagc catttattta agctaactat
    23521 atgagaggat tgatattaac ttgtatcctt ctgttcttat attttcctaa ttttgtttgc
    23581 tgctaagctg gatctttctt attacccaac aacccatcct ttactatggg ttgaggatgg
    23641 gagatgaata aggaactgga aaccgactgg taaaagttta atccaaataa tgccattgag
    23701 gaaaaatggg ttggcaaatt ctttctttgc caaagaatgc atagagctct gaaaaataaa
    23761 tgcgtttggt ttattgcaaa ttagatttcc acaactaaca taccaaatct gacttttctg
    23821 tatagaatta aaaacctctt acatatctgt cgtttcaacg gaactttttt ttctcgcgtg
    23881 agaatatttt tgggccaggt tacaacccgt tgttaataac tgtcattcct ccgtaatctt
    23941 tactccaata tatgattaat gaatttggat atagtattaa acctcatttc taaggcatta
    24001 ttggggataa tacatcacaa atatagaata caaaacaggt ctgcaaaatt aaaacatttt
    24061 gaatttttcc cttgacagga tattcttttt tcagcttaaa gaaaacatta tattttattg
    24121 gtatgaaaat gaaaagaaag atagtttttg ggtttatttg cctcttctta tgcggtagct
    24181 gttggcttac ccgtaaaatt gatactcatc aatttgcgct taaagagatg actcaatttg
    24241 ctactccctt aaatggatgg ataacagccg gtttgaaaga agaaggtgag gactttttca
    24301 tttgccattc tcaatatgat gaaaatttat attttgtttt ggtaaatgat attcaaggca
    24361 aaaccacctc ccaaatcaat ctccccagca aaattataga tttgaatacc cttattgata
    24421 aaagaaataa tgaccaatgg ctatttatta gcagtaacga ttccgattcc ctttatttaa
    24481 tgggtgttaa atatatctgg caaattcctc ttcaaagaga gaacaaatat ttttcctctt
    24541 tagagcggac tgactgggca aaaggcagag ttgattatat atataacggt tctatttatc
    24601 cgatttttct ggaggacata gataatgacg ggaaaatgga tctgctttgc cagttaattg
    24661 atttttttac tgctaaacca agaggtttga ttttatacga ttttgaaacc ggctttatca
    24721 aatggcgtta tgagttacct tgcaatccca ggagtatttt atgggatgat tttgatggaa
    24781 atggcagcaa agaaatcatt gtttctaatg atgctttgaa aaatactacc gaaattatca
    24841 atgatgtgga tgattgtagt ggttggatta ttgttctttc tgcaaaagga gatctcttat
    24901 ttaaagaaaa agaatttagc ggttatggca gaatttatct tgatgctgca gatgttaaac
    24961 aggatggaat taaggaaata tatgccgtaa actgcacctg gggaagcgaa aaacacttca
    25021 atattgcctc tgtctttaac tggaatggca aacgactgat caaaaataga acattggaac
    25081 ttccttcaac tttggaatca aaccaatttc ccaatttttt acaacaaatg gatagcaggt
    25141 ctgattatta tcttcatttg gtagatacta aggcaggatt acttattctg gacacaaatt
    25201 tgaaccccgt aaaacataac tataaggact ttgttaacca aatttgggat attggagacc
    25261 tggatagaga tggcaacaaa gaactgtttc tgcaaaagaa agatggctct ctggaaattc
    25321 tggataaaaa ttattaccgt agagcaagaa ttaaaaatcc ctttccggat accataattt
    25381 ataatgcaaa tattgtaaaa acgggatttg agacaaagcc attgctctta gtgggaacca
    25441 ataaacaaat caattattat gaatatacat ctctttccgc ttttactatt ctgctttctc
    25501 tcctgcgaaa ttatgctctt cttttgttca ttctggctgc cttattctgt attattttga
    25561 ttatcagaaa tatgtatcat aaaaaaaaca ctttaaaact aatagatagc tgggaacaag
    25621 ggattttaaa ggtaaacaaa aaaggcatta tcaaatacca taatttcaaa atcaaagagc
    25681 ttgctaatga ttttgaactg ctaaaaagaa agaaattgga gcattgtttt cctgctttgg
    25741 ctaaaccttt tcaggaattt tgcaacgcca atttgaaaac aggaagttat attggaaccc
    25801 taaatccaaa tccgcaaagc aaggtctatc aagtttactt tattcggatg acttcctaca
    25861 gaaaattcta ttatctggtt atccgaccct atacggaaga aggtgcaatt gcagaaaaaa
    25921 tggaatgggc agaaactgct cgtcgtcttt ctcatcatgt tcgcaggtat ataaccaata
    25981 ttttgctcgc tctggaaact cttaacacag atacagaccc cacccgcaac aaatattatc
    26041 aaattatccg ctccgaaatt gacaaggtaa gggtctttac tcacgctttt caacggttta
    26101 cggaactgca agattatcaa ttggaactgc aggatatcat tccttccatt gaacactgtc
    26161 tatcaaaaat tcagtttccc gaaaatatac aattgattaa gaattggtcg ctggcttcca
    26221 tttctgccta tattgagccc attcgttttg aagaagcact cactaatatc gttaataacg
    26281 ccttggaggc aatgcctgaa gggggaactt tacatatcag tgttaaggaa tttcctcttg
    26341 cttcttcacc ccaaggtcaa ttgagagtgt taattgaaat tgaagatacc ggcaaaggta
    26401 ttccgcaaaa atacctggaa gatatctgga aaccgttttt tactaccaat caatccggaa
    26461 caggtatcgg tattccggaa actaaaaaaa taattgattc tatgggaggg ttaatgaata
    26521 ttcaaagtga agaaaatatt ggcactgttg tcactatttg gctaaaagga gattctaatg
    26581 ttagaaatta gtatcctttt ggcagatgac gatcttattt tccttcagct gacttccgat
    26641 tttttaggga aaaacggcta tgctgtggac tgtgcttcca atattgaagc aacaaagacc
    26701 cttttacaaa aaaataatta tgatattatg atgctggata tgtgttttcc tgctctttat
    26761 gacggcttta acctgttgga tgaagtgcac gaaaactacc ctgatttgcc tgttttaatg
    26821 atttccggta gcggacatat ccccgatgcc gtaaaagcaa ttcaaaaagg agcggcggat
    26881 tttattgaaa aaccaataga tactacccat ttgatcttgc gcctggaacg aatctatgat
    26941 ggaattgtaa ccaaaaaaca aatgcagagc ttacgcttag ccacaattgg gatgcaaggc
    27001 aattctgcta ttatgcaaaa catctatgac gatatctgta aagctgccag atttgattgt
    27061 cccgttttaa ttacaggtga aactggtgta ggaaaagaac ttgctgccag agctgttcac
    27121 cgcttaagta aatatagcgc aaaaaatctg gtgagcataa actgcgcttc cattcccaaa
    27181 gaactttttg aggcagaact tttcggctat gaaagtggag catttacagg tgctgtaaaa
    27241 gcacataggg gctattttga atacgctgat aacaattctc tctttatgga tgaaatcagt
    27301 cagctccctc ttgaagttca ggcaaaactt ttacgcgtgg tctcggaaaa tgaaattcag
    27361 aaaattggcg gtaaagttca aaaagtgaat gtccgcatta tcagtgctac taatcaggat
    27421 ttggaaaaac ttaccgcgga aaaactcttt cggaaagacc ttctctatcg tctgaatacg
    27481 attgaaataa atattccccc tctcagaaaa cggattgaag atatccctgt tctggcagag
    27541 ttctttatga atgaattctg tacccggaat gatattcccc ccaaaccaat tagcccttct
    27601 gccgtttcct ggctttgtaa acaaccttgg gaaggaaatg ttcgtgaact gaaaaatgca
    27661 gtagaaagag cggtagtttt ttccaaaaat gatcacttaa ctatggtaga ttttactaca
    27721 ccctctgaaa gtgatattac tgaccgcgag tatggctctt tgcgcaaaac aattaccaat
    27781 tttgagaagg tatatataga aaatgtttta aggatttata attataatgt tcgccaggca
    27841 gcataccatt tacagatgga taaatccaac cttttcaaaa agatatcggc tctgaaaata
    27901 accctgccca accataaacc aatctaatct aaaggtcctt tgtcattcct gcacaaacag
    27961 gaatccattt tcactgtggt aaattttacc ccgccatcct ttttattatc tgcataacca
    28021 ccataattat caatgtatta tatttgtaaa ctggtttggc acaatctctg catttaagaa
    28081 aaacgaatct ttatattctt gggtttagga aagtaggatt agggtctgta ggcacggtct
    28141 gtaggctgtt cttttttcct gctgttttcg tgtattttgt ttcccacaga ttacgcagat
    28201 cttattattt gaagaacaat taaagtgata ctataacttt catcgtattt ctaatgttct
    28261 atctcacttt ttcctatcta cttctcaacc tctcgcctta caaatacctc agcatttctc
    28321 tatccgtcac tcctgcataa agctcaatat acggtttcac tggactgata ttaattttgg
    28381 gaaaaaatac ttcttccacc tggaaaaaac ctaccgaagc attcatttcc acagtaatat
    28441 tgatgggctt ttcctgcccc ttaaaaattt tcactttttg aatagcggaa gcagaagtcc
    28501 tgtgaatatc tccaactctg gtttgagaag aaagcatcgt aggaattctg gctctgcctt
    28561 tttccatatc gcttccatct ccaattaaaa ccaaacccgc ttccaaagaa tgaattttat
    28621 gggttgccat atgaccaaaa ataccttcaa tggcaagaga acgaatagct gtttttaaaa
    28681 caatttcatt ttccgaatgc aaagtgttta aaactgtatc tatataagga atagcaagtt
    28741 gaatagaaag caattcatgg gactcgcggg taattgccat tcccaaatca tgcagcaaag
    28801 aagctaaaat tacagctgta agggaatcat ccgcagttcc tgctccttct ttttccagat
    28861 tcattttaat gcccgaccga ttgagcaaat tgaacatttt aatggcgttc aatgttgctt
    28921 tccgcatatg gacaggaccg tgatcattaa agcccaaacg cttaatagat acaatattgg
    28981 catattcttg acctgcttgc agttccggat tagcaaaaag ccattcagct gttttaagtg
    29041 cttttccaga aagcaacttc agaattcttt gctccaactg ttgctgtttt attgatttat
    29101 acattttatt aagctcctaa aatctgttca tcagtttgtt gattaaattg gttactgaag
    29161 acacaatagc aattgcaatg gaaatggttc cggcaataag cagggtctgt aaaatggctt
    29221 tggaagcgtg caaattatcc ccagctatgt ataattgcat agaattaaag agacctcttc
    29281 ccggaactaa aggaataata gcacaggtta caaaaacgga aaccggtgtc tttaaacgcg
    29341 gtgctactat ttccgaataa atgcacacca agataatagc tacaaataca gaaaaaagca
    29401 ttgacttgct ataatacaaa ataattaaat aaaaagccca acacagtcct ccgctgagcg
    29461 ccgtaaaaag aattcgtatc ccttttaggt tggaacgaat ggaaaaacct aaaatggcta
    29521 aaaaagccca aataaattgc attaaagtta aagtatcaaa cggacgcatc aaaaacccca
    29581 cattgaccaa attttaagca ttgacgcact tcccgctacg attcctactg taataaatag
    29641 tgcttctaca gctctggcag ttccagcaac aaggtctcca ctcattgtat cacgaatagc
    29701 atttgtaata gatagccctg gaacaagtaa cattattcca ccaatgatta tattatctaa
    29761 acgaatataa ggtatgaaga tatccaatgt ttttgccaac ataacaatca acgctgccgc
    29821 tatggcattc agcagaaagt tattaaaagc caatttctcc agatatttta agccaaaagt
    29881 aacaactgca ccaatgaaaa aagcaacagc aaattcagcc caggaacctc caaaaagcaa
    29941 gcagaaaaat ccacttgtga cacctccaca gaggtctctt atccaaggac tccaagggtt
    30001 ttcattatct atttttttca gttcgctatt aaaattaacc aattgttcct gcaattcctg
    30061 ttcggaaagc gctttaactt ccatacaatt caccattgtg ctgatgcgag ttattttccc
    30121 caggtctata cgccgatttt ctattctttt aatactggta aaaatattgc ctttttcatc
    30181 tttgatgctg ataaaaatgc cggtaggagt tacatacgat tctgtttcag ggtagccaaa
    30241 acagttgcag acctttttca tcatcaattc cacgcggttt gtagttcctc cgctttgtaa
    30301 aattatgcgt ccgatttcta aagctaattc aagcacctgt tgagaactaa ctttgggcat
    30361 ttttctcctt gggtagttgt tgtaaaattt cagctggtaa acctaaaatt ccggcaatgc
    30421 gaattgcaca tttaggaggt gctgtctttt cagaaacagg taaaagagaa taatccattg
    30481 cttccgaaag aagtgccaag cgtgtttcca aattgctgtc ctgcattttt tccaaacgct
    30541 taagaccttg ctcatcaatt cctttgatag taaaatgagc caaacctgca agttctgccg
    30601 gagcactgaa atgagtagca gaaatgatag tattattact atttatcaaa tacttaagcg
    30661 ttgctacaca aattgcttcc ccttcttcgg gattggttcc tttagcaaat tcatccagta
    30721 aaacaagttt atttccctta cgcagtaaca tattagataa agagacaact tcccgggcaa
    30781 aagaacttaa tgtctcactg ttttctcccg catcgtgatt gtaataaacc tcatcatata
    30841 gaggtaattt agctttttta gccggcaaag gtattcccag tgaagccaaa atgcacattt
    30901 gaccaatagt tattaaagca gtgcttttgc cacccatatt aggacctgtt atcagattat
    30961 cctttttgct cattgtaata tttaggggtt ggtattggcg ttgcaatgtt tccagaaaaa
    31021 gtttcagagg caaattaacc gcttcggtaa gataaatacc ttcaaaagaa ggagttatat
    31081 ttggcaggca acagttataa tgcaaagcaa atttagctaa gcaaaaatcc catgcatatt
    31141 ctccggcact gttataggca agctcaaaat tactaaaggc attttgtatt tgagttgtaa
    31201 gagagcataa aattttattt tcctcttccg tcagctcaat tgagagttga tgcagttcgg
    31261 ctttcaaatc agcagtttct ttagaatcag ccaggcggaa attataattt gccagccctt
    31321 ccgagaccac aataaaatat ttggatgcta aaattgcctc cagttgttct ttacgattgc
    31381 gggaaagagt aaattcctct ttcaaagtag gcatacccaa ttcctgtttt gccttttcca
    31441 aatcccgttt ccgagcttct ttcaaacgct tagctatagc aaactgcttt tgggtaagct
    31501 tttttaagac agaagaatag gaaggataaa tccgaaaggt aggaagttta ttgccttcgg
    31561 gatcaagcag ggaaaacatt ttttgcatat ccggtaacgg atgttgatga ttaagtttat
    31621 gttcctgtaa tatttcctgc aaatgcaaat aactgaaaag aaatgatttt atctcaaaaa
    31681 gttcatttag ttccaaacgc tcggtggtct ttaaatgctt tatgggcaaa agcgaaagtt
    31741 cactgaaaat actttctaac cttgcttcca atgctctact ttctttgatt gcttttaata
    31801 aattttctgt attccggttt tgagttttca attcctcaaa gctgtagtgc tttttattca
    31861 gtttttggtg tttaacgctt tccataactt cgccattggt ttccagcaag gcaaaaagtt
    31921 tatctaataa cagcgcttct ttcacatttt tttgcattat ttcatcctta taaaggcatt
    31981 ttatattagt tacttctgct gtcaatcatt tttctgatag ttccgtttcc cgtgatgagg
    32041 attttggttt gctgtttttg taagcgcttt attgtttcgg gatgaggaaa gtgataaata
    32101 tttctgctgg aggcagttat ccaaacttct tgagggttaa ctgcctgtaa aaattcggac
    32161 gaactggaat tgcgggaacc atgatgtggg atttttaaaa agtccgcttt cagttcttgc
    32221 gcatagttat ctaccagata attttcagct tcggcttcaa tatcccctgt aaaaagaagg
    32281 cgttcctttt ttgtatccaa acggcaaacc agagaagagt tattctcgtt taaaggatga
    32341 taggatttat ccggatgcag gaatttcaat tttgccttgc ctaaaaagaa tgaacaggta
    32401 tcagtaactg tatgtatttg agctgttcca aaatatttct gtttagatgc atagttccag
    32461 agctcttgtt tggtgctttc atcgctgaca aaaatatgct ttattttttt atgattcagc
    32521 agtgttatca aacctccaca atgatcgcta tgtaaatgcg taagaacaag atagtcaatt
    32581 ttggtgatgc cttttttgcc cagccaggga agtaaattct tacttaacca actctcttcg
    32641 gaataatcgc ttttatttaa aatattttcc gcaaaccctt tcttctttgt gccacctgta
    32701 tcaatcagca ttgtttcacc atttgccaaa cgaattagag tgcaatcact tacaccgcaa
    32761 ttaaaaatat atacacttgc ctcaggtttt tgccaagtag gaatcagcat aaaaccggta
    32821 accaacagag acataggaat tacggcttgc aaagcaaatt tgaatttacc gcgaataagg
    32881 aaaaaacccc acagaataac aactgctaaa gccattgcct gaaacagaga taagtaattg
    32941 gcagaaaggg aaaaaggcaa gttgtaacaa aactgcatcc agcgtatcca taaaatatta
    33001 actgctctat aaactataac aaatattttt gttaaatacc agctctgagg agttatcagc
    33061 actaaaaaag atagcgggag caatattccg ctaagtggaa tgcctaatgt attgccaatt
    33121 ataccattta aagatgcagt cccaaaataa tagagagtga aaggagttat tgctatggaa
    33181 ataagcgccg aaagtaaaat accatcttgt aaatcattaa gcactttata aaagggattg
    33241 ctcaatattt tgtcccggga aaataaccga aaattgggca atccgtaaaa aataaccagc
    33301 accgaaacaa aggaaagctg aagtcctata ttaaacaact gcaaaggact taagagagtt
    33361 ataataaaca aagataacgc caatgtttga gctcccgaaa caggactttg cagccaccga
    33421 gctaaaatac ctactgaaat cataataata gaacgagtta cggaaggagc ccagttattg
    33481 agtgccgcat aaagtaaaat taacggcaga aaaagaaact ccgctatttc ccttttaaaa
    33541 aacatcctta acagcgaaac cataatcaaa taaataaacc agatatgcat tccgcttaca
    33601 actatcaaat gtataattcc tgctccggta agctgagtaa tgtattcctg ctttaatccc
    33661 gattcaccaa aaagcaatgc ttttgcccaa tccgcatctt ctcccaattt agcttccagt
    33721 gaacttagca gttcataacg caatctgcca attatagcgc taacttttag gttgggaaat
    33781 tcctgtgctt tgcccaattg ataagcaact gcagaataac gattaggata aatatccaaa
    33841 ataggatcgc gggttaaggg ataaatttcc gctaataaac gataagttct gcctgggatt
    33901 aagttttcat tggtagatag gataattttt tcttgcaaag gataagaggc aagggaatca
    33961 agctctatgg cataacgctt ttcctccact gaaaagaccc ttgtaacatt aaaagttatt
    34021 tcctgctgaa ggtgttcttt ttcctgtaat aacatcgcca gttgagaact ttcctgttgg
    34081 cataaagaaa agcgtaacat acctaaaaac aaaaaaagta acagcagtaa aatttggcgt
    34141 atcttacgca taaagatagc aacaattgtt aatccaccgg ctataattaa ttgaaaattt
    34201 attccctgca ggaaacattt tgccaggatg ataccgatag tccaaaacaa aaccggcaag
    34261 agaaaaggag caggtgcttt tctgtttgcc tccatttaca ttttatctcc atcttcagga
    34321 ttttttattt aagtcaggag tcattagcgc tttcccaatt ttatctctta caatatcttc
    34381 attggcgctt atgactaatg acgacgataa attattctct cacagatttc atagatgaca
    34441 cagatttata attttttatc cttcccttat aaacaaaaat ttatcctaac tgccatttga
    34501 tattcattct tccttcctaa tatcacgcgt tttcaatgcc aaattcattt gtaagtagcg
    34561 taaaagatat tgcggcagtg aactacgaaa atgtcgttct tccaggttaa ggtatatgtc
    34621 ttgtatagag cgctatttcc actttataag gtctttcccc ttcttgtaaa gctcgattta
    34681 gtttatccag cattttagtc cgtttgctta tactatttgc cttcactttc acactaaata
    34741 aagcgctggc aatttcttcg ccttccacat cccaaaccct tgccattaat gcaatttcct
    34801 ggtctctttt accggtattg gcaagagtgt attccagttc tataaattga gtgccgaaat
    34861 tggagatgcg gacatcgctg atttgaattc gggacgcatt tcttcttaca ccggagcgtt
    34921 gaatgaaaat ccccagaata acaagaacaa taacaataat caggataaac tgtttcggtt
    34981 tataccgctt cgggaattca agccgatcaa ctactctcat ttttgaaccc ttaataaata
    35041 aactgccaag cagaatagaa ggtattttgc cagtcatcaa ggtgagaagt ttcaaaacca
    35101 aaaaggagac ggtgatgagc aaaaatatct ataaggacat tacttttcaa ggtaatagtt
    35161 tctgtctttg ttccttgaaa ccaatccgcc gtgccttcat caatttcttc cgctgtatta
    35221 aaaacattat gataattttg cctccagtca ctgccaaaac ttccctgttt gcaataagat
    35281 acgcctccct gccaaagaaa aatatcttta tatggcaaac gcaaaagaca agaaacatct
    35341 accaaattgg aacctttagg atagcccaga ggtctgccat cagtagaaaa catcgtatga
    35401 ttcaaatagt gcgtataagt gaaaggacgg acagctgtaa tttctgttgt tatatctgca
    35461 gaaggacttt ccggtaaaga aaaaggaaca ttccaacgca ttccaccctg taaagcatat
    35521 ttatttcccc accaatcact gaaaagtttg ctggaactaa attcatctat aagaacttgt
    35581 gcataaagaa ataacggttg aacaggacga taagaacctc cggcaaaaat cattacatta
    35641 tctctatccc aaaggttatg ctcaattgct cgccagaaag cattaggtaa aaagtagcta
    35701 atttccggtg aacgattgcc atagatgatt tcttcaccaa ggaaaagatg caaattagaa
    35761 caaggatgaa agccgaattg atgcaatgcc agatacttat cgggataatt tttgttgttg
    35821 taaaaatggt cattatatat attgtaagtg ctatctgcca ttaaagatgt gtgtaaaaaa
    35881 gagatactaa aaatgcctgc tctgccttct gccaaaatat aaccgtaatc attcacccca
    35941 tcattcaaaa tgatacttcc gctaatagaa ttgcctacgg gaaattttcc ccgtcctaaa
    36001 gccagagtta agttcttggc attgtagctg atatctccgc taagattatc aatgttcagt
    36061 ttttcatcgg tggtattgca gtaaccatcc accagccagg aatttcttgc tgcgttttga
    36121 ttacccaaaa aaattccgtt ccaccattta gtattcattt ctatgtgttt tccatattgc
    36181 gatttcaact gccaccctct atagagaaag ctgtaactat catccttttt aacaggttcc
    36241 aaacccccaa taaaattcag ttcccctcgc aatttataat caggactttg ataactcaat
    36301 atagaataat tggtatttcc caaacgacaa atatcaagaa cagcgtcacc tacgctggaa
    36361 cgcgagacaa aaggaaggta taatccatta ttataaatag agatactatc ttctttagcg
    36421 cgttggggat aacttaataa actgggtttt aacccttcct gaatttccgg agcaaaggca
    36481 gaatccggta tcaaacgcaa agcaggtaat aatgaacaaa aaagtaacag gatagccaaa
    36541 catattttct tagccatatt ttattgggat tcatcctgat tacacaattg caccaggcgt
    36601 tccacttcct cctcggatat ttttaattct ttagcaattt cactatttgt ccagccaaaa
    36661 tcaagcaaac gacgaaccat aagacagagg gtattatttt cctcggggtg aatatcttct
    36721 ttattttcgg gttcaatatt gatgttcagt gttgttttcc ttttctttct gctaatgctt
    36781 gcaatgaaat gatatattaa aattaacagc aaaatcagga tgattaacag cataattccg
    36841 ctaatccatt ttttcccata taatttttta attttagcgg gtttagttgt ctgcttctta
    36901 tcctgctgag ttttgggcat aatatatagg gagtcggttg ttgttttttc ttcaacggag
    36961 cttattttgc aggcattttg cgcgttctgc ggggaacata attgggctga atcttttttc
    37021 tcagtcccct ttagcatttg ttgcgccaaa gaataatact cggatttttc aggaattttc
    37081 cccactattt cctttagccg accactacct ctctgtttta ataaacaagc ccatttatac
    37141 aatatctcat aatcacttgg ataattacgg tgaagttcgg agtaaatttt atcagcgcta
    37201 ttcaattttc ccacagaggt atagaaatcc gcaagctcta ttttttgttg tttatccggt
    37261 tgagcaacaa taagatccaa aactaaacgc cggggtgcat ccaattccat ctgttccaaa
    37321 cagtaaggac agaaagtaga gataactatt ttatccccaa attgttctat actttttatc
    37381 aaaccctcag cagaattgga aatatctact ttactgacat cggcattatc taactttatt
    37441 tctacaaagt cattactttt atcaacttta taatcccctg tgccataaag acctattacg
    37501 attcttgtag ccgctgaacc ataattctgc caatcaatat aagtaacctc cagagcagaa
    37561 caaaatactg ccgtcagtaa aataagcgta actaaaaatc tcttagtcat ttatgcatct
    37621 ctttgctttt tctgaaaagg aagtggcaaa ttccgctttg ccccttaaat ttcagcactt
    37681 taaaaggtat gctgaaaagc acttaccgcc atcttttgct atcctctttg ctatcaccat
    37741 tttagcatag caggatatta attcctgata acttcttcct ttttccaact tatattagta
    37801 tttcactctt tttcaacttt ataaaattgg caagttattt ttttcgctgg ggctgtccct
    37861 aaacaatgat agcgatgatg gcggaagcca ctcgtttaaa tgaaaaatga agaatgaatt
    37921 aattcaggag tcatcatcgc ttgtataact ttgc
//
LOCUS       DAOPFL010000006        37303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1036_length_37303_cov_93.863993, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000006 DAOPFL010000000
VERSION     DAOPFL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 37303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37303
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1036_length_37303_cov_93.863993"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="PLG69_01210"
     CDS             complement(<1..1947)
                     /locus_tag="PLG69_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPI42157.1"
                     /translation="MRKSILCLLMLIVSISIIYGDYFVNFEGTNETKTAYASGNVTLS
                     GLSWNMTEALIGNLANDWKNGTRSARLRGYGTSAMTMLENKTNGLGTLSFYYRRYSTE
                     AQVDWKVEYSVDNGSSWTQIGSPFTAPASDTPSLFSEQVNVSGNVRIRIKRATESGTT
                     DRRLNIDDITLTDYTAAEPTLILDPTVLIGFTYDLGEGPSASQSYTLSGAYLSPSSGN
                     ITITGSSSFEVSSDNVTFSNSFSLPYSGGILSPATLYVRLKAGLSEGNYLNENITHSG
                     GGASAFLAVNGTVTSPSVLLTAEGYLEDFTSFTSLESLPFGWTLSNQYTYGGDFGTGS
                     TGGLRGNGVLGIQLTASAPNNALTATLTLKNQTGATINNLNVAYQGKVARTDQPGTPK
                     WVVSVNGTEIPELEYSTAEGINQQKSAVVTGLNIAVGENIIIQWFTTSAGTSGARRQI
                     GIDDVNINTNIPVNPLINVTANLVTFQTAQGTPSEPQSYFLSGIDLSQNINISAPNGF
                     EISVDGGLTYATHTSVLPTYEGNVFVRMTGTAVGTFGGNIIHTSLGATDVYLPVAGIV
                     TGATGYAVDLFFSEYIEGSSSNKSIEIFNGTGSEVNLSDYKVALYSNGSTTPSSSLIL
                     SGTLENGDVYVISHPSANANILAVT"
     gene            complement(2091..2765)
                     /gene="tsaB"
                     /locus_tag="PLG69_01215"
     CDS             complement(2091..2765)
                     /gene="tsaB"
                     /locus_tag="PLG69_01215"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPI42158.1"
                     /translation="MKLALDTTQNFGSIALADEKRIIYSAYFDIKITHSETLMPAIDY
                     AFQFCNVERRELKEIYVCIGPGSFTGLRIGLATAKGIAFALGLSLYAFSSLELSALPA
                     SRLGKNILSAIDAKMKEVYFAYYDQAIKEIIPPAIMKPEDLCQLHLDDFILCGSATEM
                     LSPLLQKAGYNFHNLNPIMKIPSAAGLFFLPEMLPEKYVPQDIEDLEPMYLREAQAQV
                     KKKKEN"
     gene            complement(2762..3214)
                     /locus_tag="PLG69_01220"
     CDS             complement(2762..3214)
                     /locus_tag="PLG69_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPI42159.1"
                     /translation="MQRKDFEEILDFAIAREREAIKFYQNLQNQAQFQDQKEMLKELE
                     AMEQNHITIIEKLRQTGVKDEDIHKTPNLKISEYITADPETLDLSYQNILIKGMKREE
                     TSFLLYSEMSVKFPDPEISTLFRRLAADEAQHKKYFENLYDDWMRKGN"
     gene            complement(3295..3435)
                     /locus_tag="PLG69_01225"
     CDS             complement(3295..3435)
                     /locus_tag="PLG69_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPI42160.1"
                     /translation="MQKYQCMACGWIYDPAENDNIPFEDLPDDFVCPECGVGKDMFEP
                     LD"
     gene            complement(3456..4046)
                     /locus_tag="PLG69_01230"
     CDS             complement(3456..4046)
                     /locus_tag="PLG69_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPI42161.1"
                     /translation="MPNKETRTAENLMKSFAGESQARMRYLYSAKTAKNEGFEQISNI
                     FTETAENEKEHAKIFFKHLQKMGLEGEIINIISSYPVGWSADSTLKNLEYAANGENEE
                     WTELYPSFADVAEEEGFSDIAHSWRMVAKVEREHEKRFRKLYDNIKNQRVFIREEKVF
                     WKCLNCGYIHEGTEAPKSCPSCNHPQSYFELFMENY"
     gene            complement(4066..4443)
                     /locus_tag="PLG69_01235"
     CDS             complement(4066..4443)
                     /locus_tag="PLG69_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPI42162.1"
                     /translation="MIALRQMWHCPICGNVVEVIYVGGGELVCCGQPMQLLKENTVDA
                     AVEKHIPVVTDLGDKIEVSVGSIPHPMEEKHYITCIEVLTEKKVLRKELKPGDEPKAK
                     FCVKMDKVIAVREYCNVHGLWKA"
     gene            4607..5335
                     /locus_tag="PLG69_01240"
     CDS             4607..5335
                     /locus_tag="PLG69_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42163.1"
                     /translation="MKRLVIMLLALLLLAGCVQYDEELWINRNGSGHATIRVVHRSPY
                     ENPEEIMRKAALPGINLQSYDIQRKGPDVIYTINFKFKSIDAFNNVNDQLGAADFWGK
                     ITLNKEPGRRITFKRRIALGSQEQELEEEGIENILGLLQPENPVWTYKVHLPWKIIST
                     NALPENVDRENGTVTWSFDTRKMWHKQELMTVELQKDFPWFLLVIGAVIVVLLIFLIH
                     WMFRFPKKSHWRERLKHSENAEKS"
     gene            complement(5435..8191)
                     /gene="polA"
                     /locus_tag="PLG69_01245"
     CDS             complement(5435..8191)
                     /gene="polA"
                     /locus_tag="PLG69_01245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPI42164.1"
                     /translation="MADKLYLIDGTALLYRAHFAFIKNPLINSKGMHTSAIFGVINSF
                     LHILENTQAAYVAISFDRKAPTFRHNLYEAYKAQRPPMPEELTAQIEPVKRFFELIGL
                     KEIGLDGYEADDILGTLAKQYKDQFQIVFLTSDKDYCQLVDERSVMLDPMKDIVLDRD
                     AVYAKYGVYPEQFIDYLALVGDPSDNIPGVSSIGPKTAAMLLSEYKTLDNIYANLDKL
                     KPRLQEVLRENKENAYLSRQLAKIDTDVPIPKPDLEHLTFWAKDLRKAIPLLEEYEIN
                     SIKRKIELKYPETNATKQQTEYQDDIFKEKEKPQEDTAETEEKMPFSAVLVNTLNFVT
                     LLKELKEAKQISLDTETDSPEPMRANLVGISFCMDEKKAYYLPLGHQLAVNLPLPEVL
                     RHLEDALKGKLLLGHNLKFDLIVLARNGLDLDNPLFDTMLAAYILDPGTLNYSLETCA
                     LNELNYKMIPISDLIGKGKNQSTFDLVEPEKACIYSAEDAWAVYKLYPVYRRKVDFSV
                     MATLFDTIELPLIKVLQQMEMNGVAIDCSVLSEISHQINLELKTLTDKIYAFAGYEFN
                     INSTQQLAKLLFEEKKLPYGRKTKTGYSTDNSVLEELAIDYEIAGIIIQYRQLSKLQS
                     TYVSALPKMINPQTGRIHSSFNQTVTSTGRLSSSNPNLQNIPVRTEIGREIRKAFCAK
                     DAEHLILDADYSQIELRLLALMSEDEVLIDAFKQDLDIHKRMAAKIFNVDIEKVTPDQ
                     RRAAKTINFGILYGMGQRKLSRELGISLNEAKDIIDGYYAQFPSIRNFINQCVNKARQ
                     QHYAETLFGRRLYLPNIDSKNQGLKSEAERIAVNMPIQGTAADLIKIAMLNIHKQIDK
                     REDIKMILQVHDELLFEVQQEALEETKNIVKTYMENALPPKYAEKVHLKTDVGVGKNW
                     FEAH"
     gene            complement(9679..10767)
                     /locus_tag="PLG69_01250"
     CDS             complement(9679..10767)
                     /locus_tag="PLG69_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42165.1"
                     /translation="MKRYLYVMLYILLIMGCSTVPKVVPHPWNKTLSDEEIIQGSTIA
                     IETTILSTPLIGSQELLEEEIAQKAKSLLERRGFYISNSNYQYKMKVLFSVEKQLETI
                     TTSMIQNSNYSGSAMTVKSGYGITLANLISETIQVSKSEVSTNQYDTYTYSFACEIYT
                     ASGKLIWKNDAIQKAYKFNVLDYITPMLQIAFSSLPKDENIYPRVPKIKETQVKNFLS
                     VKVYQTYLRCPALPNLICFEDYWLDDYEYKPHKFKQNYSKNPQAFMAYIDLLQTAEFA
                     IPGSKIKKWDNPLDMNNWRTVYLIGKYYLGNDQNPVNIVVKLKGTPSFYVVSKCNIVT
                     DEEYNSYYQKYNLWINKLTEFFDFFEKG"
     gene            11182..11655
                     /locus_tag="PLG69_01255"
     CDS             11182..11655
                     /locus_tag="PLG69_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPI42166.1"
                     /translation="MERPSWQQYFMEMAYLASKRSTCLRRKVGAVLIRDNQIIATGYN
                     GSPKGVPHCEDIGCLREQQKVPSGKNQELCRGVHAEQNAIIQAAINGSSTRGAILYCT
                     NQPCSICARLIINAEIKTVYIAESYPDELGEELFREAGVELIKFDLQSNTLKKLI"
     gene            11655..12194
                     /locus_tag="PLG69_01260"
     CDS             11655..12194
                     /locus_tag="PLG69_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPI42167.1"
                     /translation="MSIFRRFTVKDLIIITSIAALGIAIKPIINPVTKLFSTPLGIPG
                     GSITGGLYMLWLVLAVVIVDKPFTGTLYGLLQSILVLLIGMAGKMGAFSLVAYTAPGI
                     VADLLFWLLPYKDKLITHLFLCGCANLTGSLISSAIFFQMPTVMIVFNTCMAFSSGIL
                     GGYLGFGIYQSLKAARIIQ"
     gene            12191..13117
                     /locus_tag="PLG69_01265"
     CDS             12191..13117
                     /locus_tag="PLG69_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42168.1"
                     /translation="MKKYLCFLLLSGVMMTLSSLEVIDTKGISHFYSNAELYKKETQE
                     IKTSREKDGTVRLNTWQGFRFDIWLKEQNLGDFATIRFESSDRYQVTLEKSEFDSLES
                     YLVTGQDGIPFEENMLRLIFPALREMQWIKGLERVVLEDFQPLIRPQRFYLLQETLKK
                     YPLQQDPEPFVKTQGWYLRDILSDLSEAEEKQVILYSRDGLKQHLTYPLHLSGAILEK
                     TAEQHYNLKSPQIPGGMWMKDVIYLQCDNNALISEDSINQLIRLAKILHWETTPEMQF
                     RIVFEDREEVMNFTDALAEPQVFRGALYLELF"
     gene            13117..14466
                     /locus_tag="PLG69_01270"
     CDS             13117..14466
                     /locus_tag="PLG69_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI42169.1"
                     /translation="MLFLQDFGISFAEQKLWEGINLKIPEGSIFHLKGPNASGKSSLL
                     NAISGIIPEYVKAKTTGNILLGQTDLQAIPLKEKFHYLWHQLSDAEAQLFFPDCLSEL
                     AFALEHLALPAENIVAKINLAAAQFGLEKMLQRDPSTLSGGEKKLLLCAVAETIDPPL
                     LLLDEPLNGLDKQATDLVFKWLKNKKEQGKIIVVADHNPLMETVADFSYTLPENPQPL
                     TIDNSLFTEAVLNLQDKRDCAIKPIPDVLYNIEELSFAYPNAEFLFKDLSIKINSTEN
                     ILLKGKNGAGKSTFLKLLTGLLKPNKGKIYLQGKLLQGLNPKNFAHFYYQSQITKENL
                     LGISVKQNWLFWQLAIPELKDLPIKEDPLFTELSAGQQKMVSQQILPYMLSKFWILDE
                     PFASLDENAGKQLFNLLQYKSRNYPGMLIVSHSLEAHRELFDRVFSFQNQTLQEEKG"
     gene            14463..15188
                     /locus_tag="PLG69_01275"
     CDS             14463..15188
                     /locus_tag="PLG69_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPI42170.1"
                     /translation="MKKSSLHPLTHILIALLLSTFVFIAKKPVSLLCLTILALLYAVW
                     RMENGWQKVFKTLWHTLPFLLVLAFFQIVFRRTGTLLWSYGILKISEEGVLISLQLTL
                     RLLTVIYCAKALAVMDYTEFSKAFSAIRLPEELSFMLSYAIHLVPKFLGQLKGFVTGL
                     KLRGIDAAGLSFSKRLQVYKQLSITALAELIRSSETAAIALELRGFRSEGKRSKLHRQ
                     KFSYRDLLCLLFFSVLVIIVTRI"
     gene            complement(15572..16816)
                     /locus_tag="PLG69_01280"
     CDS             complement(15572..16816)
                     /locus_tag="PLG69_01280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPI42171.1"
                     /translation="MNWGKDILLAFIFLLLTTFCLISLMGINPKHPAEIKPDLQKPSE
                     PWIIQTIPEGGSIFSVLANLNLPLKEVGLLAFKFGDYIDVSTIQPGDTLKILLSEDKQ
                     HIAKMMFVQEPTTRHHFTVSADSLVYSLEALPVKRVKRILEGKLEGTLDASLLAMGFA
                     PQDKQAINNGLETEINFARDARNGDFFRVFVEERIFEGKTLPGRKIFYVQYSGERTGT
                     CELFRYEDEEEKSVLNGLYTKEGKSCHSNGVGFPLSVIHLVSPFGRRLDPFYGNWANH
                     QGADYRAHYGTPVYAVANGKVTDAGYNGGWGNEIRIKHPSGLTTQYAHLSSMSVRKGQ
                     TVKKGQIIGRVGSTGHSTGAHLHFGLIKGKKYINPTNLKMVGTEKLNETQMARFKSQQ
                     QIILQDMENLMHPKPVIASAKK"
     gene            complement(16813..17757)
                     /locus_tag="PLG69_01285"
     CDS             complement(16813..17757)
                     /locus_tag="PLG69_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPI42172.1"
                     /translation="MTDRDNLTRVTVNLGLFSNILLSIVKTCVGILGHSAALLADGIN
                     STSDVVYYIAVKIFMKQAQKPADVEHPYGHRQLESISAIVVGAFILTTGIAIFWESVN
                     KVYDLLSKTEIGQPLSIWTLVIALFTFGLKIFLYTYTRKNIPKTKNPTLKALANDHLN
                     DIMAAVAVVVGVLLGRLGYYWMDPAAGAIVAIYIIKTGVEIIMESSRELMDYLPDEDF
                     VRELKTEAMAVEGVRSIEDLGIHRFGPYFTVNMTITVDGDISVDKGNIISDSVEKRLL
                     DKFSTGLRQVHIHFHPYFEKHSSADPDPGSKTNIGVNL"
     gene            complement(17762..18757)
                     /locus_tag="PLG69_01290"
     CDS             complement(17762..18757)
                     /locus_tag="PLG69_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42173.1"
                     /translation="MEFSWISGRRYTNIAIVGICKNAGKTTVLNYILAKYPFPWGILS
                     TGRDGETEDTLFKIPKPKVKIPEGCFFCTDAKTLLQHGSGISILTKTSFQSGSRELWI
                     VKSEKYLETEITGPASVSGQIACAELLQKMGAEKVLIDGSLDRKSIVLSNAVDAIILT
                     AGASFGNQQAIIEELKRLITLSQIGTYLSPTLQKLAEQNKILIKHKGRWKQTGLVSLI
                     ANETKLLEILSKLEKPSHLYLPGAYSSSVNNHLGKHLSGIQLIFRHPECIKLSSNELD
                     VFLKNHNPLALIPFKLKAIALNPKGIGNSDIDADEFHQNLRRTFKEYPLLDVMEI"
     gene            complement(18768..20015)
                     /gene="ablA"
                     /locus_tag="PLG69_01295"
     CDS             complement(18768..20015)
                     /gene="ablA"
                     /locus_tag="PLG69_01295"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPI42174.1"
                     /translation="MSKLIDIKSIATPEQWNDWHWQIKNRITTHAQLSKYIELQPEEE
                     AVFKDKAFSFRMAITPHYLSLIDHNNPYDPIRLQAIPRIAESHISPSDMADPLSEDAD
                     APVPGMTHRYPDRVLLLLTDQCAMYCRHCTRRRKAGEHDAPMPKENVEKALEYIKEHK
                     EVRDVILSGGDPLTLSDERLDDILNRLSKIEHIEIVRLGTRTPVVLPQRFTDSLLKIL
                     QKYKFVWLNTHYNHPNELCEDSCKALAKIAETGIPMGNQSVLLKGVNDNVDVMKALVH
                     KLVKNRVRPYYIYQCDLSEGISHFRTPIAKGIEIMESLRGHTSGLCVPTYVVDAPGGG
                     GKIPVMPNYVISQMPGRVILRNYEGFITAYTEPEFVEQDKSKYRELCADEYKSTEGIM
                     ALMRGKKVAIGPAETKRNRRRKH"
     gene            complement(20030..21088)
                     /locus_tag="PLG69_01300"
     CDS             complement(20030..21088)
                     /locus_tag="PLG69_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPI42175.1"
                     /translation="MKSNGCRYGTHRVIEPKGVLPQPAKILNNDMSEIWDNEMLIDVI
                     RLNIDSASFHQIKNKLIAQGHQDLEKAFAEHAIELTNRTGKHKNEDTGSGGMFIGRVA
                     AIGDKFEMKEEVKVGDKIASLVSLSLTPLKINKVKKVLLDKDQMEIEGQAILFSSGVY
                     AKLPDDLDENLALSVLDVAGAPAQVERLVKPDDTVVIIGANGKSGILCNAVAKERAGI
                     GGKVIGAVRNENYIPTCKATGCDEVILAQATDAITIQKEVSRLTNGKMADVVINVVNT
                     EDTELPSIMAAKDRGLVYFFSMATSFTKAALGAEGIGADVDMMIGNGYAHHHSEIALD
                     LLRRNPVLMKIFKERYAE"
     gene            21745..23052
                     /locus_tag="PLG69_01305"
     CDS             21745..23052
                     /locus_tag="PLG69_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42176.1"
                     /translation="MIITFSVQNFFSIRNKVTLDFRATSDKTLEDYYVVSVEKPKLRI
                     LRMAMIYGANASGKTNLLMALDFIKRFISDTSINKEIPIPIAPFALDEDKSSIFEIDF
                     LQKGIKYSYYLELNKKNIIQEELSYYPKGRKNLIFSRKLVDIKGFIYEYEWRNAAINE
                     KQKDALELTVQNQSILSKIATIQYSGVLQEARNWFTITLKNMLGSKTVLLGFNYFNYL
                     NTPEKEKRYKPLYIELLKRADFFIEDITFEEKIFTMAELPPEVRAKFETNPKEQEDDK
                     EQEEISSLKTEFIHKTPSETYVLNYHMESMGTQRYFGLIGILLELIYKNRIVPIDEIE
                     SSLHIDLIIHYITTFLRNKRKGQLIFTSQNTALLKEKDILRRDAIWITERNEDGSTSL
                     TSVSEYPVRKEHSIESIYRKGLIGGLPNLGTTLLEGENETKDE"
     gene            23033..23656
                     /locus_tag="PLG69_01310"
     CDS             23033..23656
                     /locus_tag="PLG69_01310"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HPI42177.1"
                     /translation="MKPRTNKPTKPFKNTVLCFCEGETEKVYLTCLKKDRYKNVHIEL
                     KPQISNADFEKTFKDIKHQLAKPKKTNYKYIFYVLDMDAIYNQNQIDEYNRQKERIKS
                     LVQAKERLTIIESRPCIEFWFLLHYKNTDKCFVNCDEIIVELCKYIPEYCKNQNYIAS
                     LYEKLKDKLETARQNSEAICKKERVDNEDYSYTQMHQLIQILDDLKK"
     gene            23821..24717
                     /locus_tag="PLG69_01315"
     CDS             23821..24717
                     /locus_tag="PLG69_01315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPI42178.1"
                     /translation="MTKTGTETSPFGTKGRINHDSSKFYNSKLYEELPIQKANDKTEN
                     PFPEEYLNKVILGSAENMHEIPDNSVQLMITSPPYNVSKDYDQNLSLKQYLQLLENAF
                     KETYRVLVNGGRACINVANVGRKPYIPLSDYISQIMIKIGFNMRGEIIWNKAASASPS
                     TAWGSWLSAANPILRDIHEYILVFSKGDFYRYKKNRQNTISKEDFIQWTKSVWVMKAE
                     SAKKIGHPAPFPEELPYRLIQLYSFKDDIVLDPFMGSGTTAIAALKSKRYFTGYEIEP
                     AYIDLCNKRISAYLDQLSIDLE"
     gene            complement(25005..26861)
                     /locus_tag="PLG69_01320"
     CDS             complement(25005..26861)
                     /locus_tag="PLG69_01320"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPI42179.1"
                     /translation="MPVKKVIIYLVIILLIVEIIFAFPFFKNLEYKAQDMLFRMRGKL
                     PVSDKIVIIGIDDATFSALNETWPFPRDYHSKLIENLNKAGAKQIIFDIEFTEKTLPE
                     SDLRLAETANRYQNVIFAGKVLTSQRPGEPSQIIPPISPITEYNLPWGIVNMEADPDG
                     FIRKYTLFENFDQTKLYSIGIASLANLRVYQNNWAEHIKVNSKALHLTDKSIPLADKN
                     KAIINFRGPAGTYPAFSYASILDDSTFFMPGYQGAELDEFYEIRDSGVLKDKIVLVGA
                     TIDELHDKFPTPYGGEWTSGVEIQANFLDMVLTGNYLKKLNPWLFLLIEVVLLIGLWF
                     LFKVLKPQFSALALLGLLIILFIINLLLFNKAGIICPIAQTIILLILIYVASILNHYW
                     VTMKEKRFIRNAFQQYLAPELVNQLLANPKSLKFGGSLQEITVLFSDIRNFTTYSEKH
                     SPQETVNILHEYLTEMVKIIINNHGILDKFVGDEIMALYGTPVPLPNHSFDACKTALQ
                     MRECLSRLQEKWQSEGIEPFEIGIGINTGMAVVGNLGSEQIFDYTAIGDTINLGARLE
                     GLNKEYNTFNHIIISEFTYEKVKDLVNANYLDEVKVKGKNIAVKIYELVSLK"
     gene            complement(26947..29340)
                     /locus_tag="PLG69_01325"
     CDS             complement(26947..29340)
                     /locus_tag="PLG69_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42180.1"
                     /translation="MKKYLLLILSIILATQIFAVALMNISPQVYSFHQKCDLKVEVQQ
                     GLSDIAKMKVYYRIGKSNRWMTEEMKQDSPGSPYFIATLPADYLTTDVIEYYFCVELS
                     QGNKEYFPPQNETVPNYILEPDIAFGEAEPGFVLLTDDPIISADDGYMLVVSFFAISE
                     EIDPASIEVWVGGKNVTSLSQISPPLIVYKDETPQSGTQKAVIRARKEGKTVHSPIWS
                     TEILPSSKALKQFTYQGTVNFASNYYNYSNDDALGYTKSDAATWADFYGNYGILNMQA
                     NLYFSSLEHSNSQPVNRYTLGFNIPHLEVFAGDYAPTFSQLTLNGKNLRGIYSRLYYK
                     SLSLSVTHGQTVRKTTNELDLDSEVDGMQKSGTFKQEAFGTRIQYCDEDNFIFGINFT
                     RHNDVISSLDSTYYQYTKVDTVGNESKIYTTNAQENAVLGIDTQMHIRKPNLVIGAEI
                     ASSLWNTNTIPGAMTQEELEEYLGKDFFLDPQDFSRLFIINKNMIPIVPGRASIAWLT
                     YVRTCFYNNLLNFQYSETGSAFNAFGSAYQIPDSKVISITDNFNVSKYFILSGGLNLI
                     EDNLMKHKSETNNTTSWYLQSLIRIPQAPYFKFAYYNNSGKNEENKDIAYEKESFQKV
                     KNKSQNLIFGIGYNITQIPYVPTQVDIGYQMGTDNSKKSDSDLTDNKNNNFNITMLNR
                     FQMIPLTLQFAMTLNNNKDLLAITNKKGKNNNFFVGATYSLWEDKIKPYTNYRIVSAS
                     GAQGDRSYQYYTLGVEAYPIKSLSVNTNIALTDFNNKDNTPGDYSNFIWRVLLSQRF"
     gene            complement(29353..30030)
                     /locus_tag="PLG69_01330"
     CDS             complement(29353..30030)
                     /locus_tag="PLG69_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPI42181.1"
                     /translation="MKKIFLFAIILILTVNLLAVESVAIISASKGKVDLRRAAKVIKH
                     KTGDFLQNNDEIRTGGESFAAYKYIDGSATVKVFSHSIVQVYASTKDKKLAKTVKLNQ
                     GSVFSQIKSDSGPFSVQTPTTVASVKGTGFMAKFDEQEQTKFIVTEGELELKILDKTE
                     TQSVAAGNTAIIQADGSFEIRPSTEEDIQEIELTELESMQEKEIRKMRIPVVAPNGQT
                     RYIDITW"
     gene            complement(30043..31149)
                     /locus_tag="PLG69_01335"
     CDS             complement(30043..31149)
                     /locus_tag="PLG69_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42182.1"
                     /translation="MKRFLFILVIALLLPTLAVAREGFSVTFSPQTINVNSFNSASFD
                     PFDPDQQPILTHLTIDTGTVPKKIDLKLVLEWNNINLVETIFSSINTQNSWKLTNRDL
                     ITNEASTYFDYKEGYAKITVKDAIENSSTLKSAVLAGYFPDGVLKFKVWVREFSTAPW
                     TNDPNLPYDEFKIIIRNAGNITLLSPGVPIGKTPALVNGIPISFLWNSQLTDFNDYKL
                     IIKEFPPNNPPTVNTIDRTGAVFYETPENTKENSGFADFLPFTDGNYYAWKVTTALAT
                     EYNPKKQNTGNNILSSNWYVFQFVDEEKAAASISEFQAHLNMLQNSILLNLYTQGYVP
                     AGIVIIDGKEFSGQEAINLIDALQGQEISVELKD"
     gene            complement(31153..32097)
                     /locus_tag="PLG69_01340"
     CDS             complement(31153..32097)
                     /locus_tag="PLG69_01340"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPI42183.1"
                     /translation="MEKIVVLKGGTSPEREVSLVSGAEIASALRNMDYSVIEIDPADY
                     PKLSDLLTAIQNEQPLLVFNGLHGGSGENGELQAALSLAGIKFTGSGFKASCFSMDKY
                     ISKLIALAEGIPVPQYILMREDLLEDYQDVEDYSGFSNQLGLPIIVKPNDAGSSVGIS
                     RVECLEDLKPAVQKALQYSHSVLLEEYIPGRELTVTIIDSEAYPVVEIKPLEGWYDYT
                     NKYTHGKTKYEAPAQIDDTVAKLLQLYALRLWKAFGLSGYARIDFRYDGLKPYFLEVN
                     TLPGMTSLSLTPMAAKAAGMSFQELLQKIIYIADKEGR"
     gene            complement(32162..33073)
                     /locus_tag="PLG69_01345"
     CDS             complement(32162..33073)
                     /locus_tag="PLG69_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42184.1"
                     /translation="MLEIELTYPKPVPNRSLNIWSNPVFMQTVAEISNFIPWHLVCYK
                     GTTIVAVLPLYEKKILGCRVLKRSSLSYYQGLNLWLEENSLPARKLLDALQITMAIAR
                     GLNSNFKRIRINLTPETYDIRGFLWNKMQAVPFYTFVHNLNSELKPLPDKRRDLAQAE
                     KQNYTFEEGLNTEEFIGLLKILKVKKNWNPDCDYQGLVDFIRILYREGMLRQMNLTQQ
                     GKIVSSNLILQDGKKVYNIYQATEDNALKKGACSLHTVKLIELLQKEGFQELDFCGSS
                     IPEIARFNSALGLQLKLFFQIRSSAMV"
     gene            complement(33073..33429)
                     /locus_tag="PLG69_01350"
     CDS             complement(33073..33429)
                     /locus_tag="PLG69_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPI42185.1"
                     /translation="MKPKLLLVDDDQLLREVIGDFLSSHGYEVDLADNADSALEKFEP
                     GKYQLALIDYMMPGMNGLELMKILLKQDPKIFCLIMTGYPTVDSAFQSVVDGASDYIV
                     KPFQLKELLNTIGSYI"
     gene            33601..33813
                     /locus_tag="PLG69_01355"
     CDS             33601..33813
                     /locus_tag="PLG69_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPI42186.1"
                     /translation="MKGKVKWFNKNKGYGFIITDDNKEYFVHWKSIVTNSPRELKVLE
                     QDELVTFDLMETDKGIQAINIIRVNP"
     gene            33878..35521
                     /locus_tag="PLG69_01360"
     CDS             33878..35521
                     /locus_tag="PLG69_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPI42187.1"
                     /translation="MPFLPVNQSDLKERDWDYLDIILISGDAYIDHPSFAAAIIGRTL
                     EAAGFRVGIIPQPDWRNDSSFTVLGKPRLFFGITAGNMDSIVNHYTAQRKLRNDDAYS
                     PDGISGKRPDRAIIIYTNIIKRLFKGVPIVIGGIESSLRRIAHYDFWQDKVRNSILAD
                     SKADILIYGMGERPVTELAILLQKGVPVSSIQNLPSTVVFSSSPPAKEDIILPEAEKC
                     SAKETFYQMTRQFEDNYRTKTIFQKNAGRWLKHNPPAKPLTTKEMDSIYALPFAYQPH
                     PVYAGHKIPAYEQIKDSITSHRGCYGGCNFCAIACHQGRNIQSRSETSLLKEAQKHTG
                     TITDVGGPTANMYASFCRLDFPETCKRSSCLVPDICPNLQINHSVQLKILAKIGKLPR
                     IKHLFIASGIRYDMAVNDDNYIEAIATKYTGGRLKLAPEHSVSSVLRLMNKPQIEVFE
                     KFCKKFYFYTNKAGLKSQIIPYLIIGHPGTTMEDAFELRNWLRKNKLKVEQVQEFTPT
                     PMTISTCMYYTGLDYETGKPIHIPKPSEIRRQKELIINR"
     gene            36013..36141
                     /locus_tag="PLG69_01365"
     CDS             36013..36141
                     /locus_tag="PLG69_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42188.1"
                     /translation="MIDIMERFEPGEKRKGHNLSLLLRMSFAGTKDINDGFEPGEK"
     gene            36727..>37303
                     /locus_tag="PLG69_01370"
     CDS             36727..>37303
                     /locus_tag="PLG69_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42189.1"
                     /translation="MQSSTYTASNIKVLKGLEAVRKRPAMYIGGTSERGLHHLVYEVI
                     DNSIDEALAGYCDEIICTITTEGNIRVEDNGRGIPVDIQKDMGVPAVQVVLTVLHAGG
                     KFDDKSYKVAGGLHGVGVSVVNALSEFLEVNIFKDGKEYFMRFERGLPVTELQVLGDT
                     NKTGTVVTFRPDPLIFETTEFSFDYLTSRLRE"
BASE COUNT        11513 a         6930 c         6890 g        11970 t
ORIGIN      
        1 tgtaacggct aaaatattcg cattggcact gggatgggaa ataacatata catcaccgtt
       61 ttccagagtc ccgcttaata ttaaagaaga agaaggagta gttgaaccat tggaatagag
      121 ggctacctta taatcagata aatttacttc agaacctgtt ccgttaaaaa tctcaataga
      181 tttattggaa gagctgcctt caatgtattc ggaaaagaat aaatcaacag cataacctgt
      241 tgctccggta actattcctg ctacaggaag ataaacatcc gtagccccca aactggtatg
      301 aataatgttt ccgccaaaag ttccgacagc agttccagtc atccggacaa aaacatttcc
      361 ttcataagta ggaagaacac ttgtgtgggt agcataagtt aaacctccat ctacagaaat
      421 ctcaaaaccg ttgggagcag aaatattgat attttggcta aggtcaattc cgctgagaaa
      481 ataactttgc ggttcggagg gtgtgccttg agctgtttga aaagtaacta aattggctgt
      541 aacattgatc aggggattaa ctggaatatt agtattgatg tttacatcgt caatgccaat
      601 ttgtcttctg gctccactgg ttcctgcact cgttgtaaac cattgaataa taatattctc
      661 tcctacagct atattcaaac cggttacaac tgcacttttt tgttgattaa ttccttctgc
      721 tgtagaatat tccaattcag gaatttcggt tccgtttaca ctaactaccc atttgggagt
      781 tcctggctga tccgttctgg caacttttcc ctgataggca acattaaggt tattgatagt
      841 ggctccggtt tgatttttta aggtaagagt ggcagttaaa gcattattgg gagccgaagc
      901 tgtaagctga attcccaaaa ctccgttacc tcttaatcca cctgtagaac ctgttccaaa
      961 atcccctccg taggtatatt ggttacttaa agtccatcca aaaggcaaac tctccagaga
     1021 agtaaaggaa gtgaaatctt ccaaataacc ctctgcagtg agtaaaacag agggggaagt
     1081 aacagttcca ttaacagcta aaaaagctga ggcaccacct ccactatgag tgatgttttc
     1141 atttagataa ttaccctcac ttagtcctgc tttcaaacgc acatataaag ttgcggggga
     1201 aagaatgcct ccactataag gtaaagagaa actattagaa aaagttacat tatcagaaga
     1261 gacctcaaaa ctactggaac cggtgatagt tatattaccg gatgaaggac ttaaataagc
     1321 tccggataag gtatatgatt gtgaagcaga aggtccttct cccaggtcat aagtaaatcc
     1381 tattaaaaca gtaggatcaa gaataagagt cggttctgca gcagtataat ctgttaaggt
     1441 tatatcatca atatttaagc gtctatccgt tgttcctgat tccgtagctc gttttattct
     1501 aattctaaca ttaccgctta catttacctg ttcggaaaat aaactgggtg tatcgctggc
     1561 aggagctgta aaaggagaac caatttgagt ccaggaacta ccattatcaa cactgtattc
     1621 aactttccag tcaacttgtg cttcagttga ataacgacga taataaaaag aaagagttcc
     1681 taaaccgtta gttttatttt ccagcattgt cattgctgat gttccataac cccgcagtcg
     1741 ggcagaacga gttccattct tccagtcgtt agctaaattg ccaattaatg cctctgtcat
     1801 attccaggaa agtccgctta aagttacatt tcctgaggca taagcagttt ttgtttcatt
     1861 tgtcccttca aaattgacaa agtaatctcc atagataata gagatgctaa cgattagcat
     1921 tagtaagcag agtattgatt ttcgcatttt atattctcct tattacactg aagacaccga
     1981 aaatatgtat tagcaacata gatataacgg ataaacacgg attattaaat taataattat
     2041 aaaattcttc tctttttctc tttatttctt tgttttccct ctcacttttt tcaattttcc
     2101 ttcttctttt taacctgcgc ttgggcttct ctcaaataca ttggttccaa atcttcaatg
     2161 tcttggggaa catatttttc cggcagcatt tctggcaaga aaaaaagtcc tgctgcagaa
     2221 ggtattttca taataggatt aagattatgg aagttatatc cggctttttg taataaggga
     2281 ctaagcattt ccgtagcgct tccgcaaaga atgaaatcgt ctaaatgcaa ttggcaaagg
     2341 tcttccggtt tcataatcgc aggaggaata atttctttaa ttgcctgatc gtaataggca
     2401 aaatacactt ctttcatttt ggcatctatg gcagataaaa tgttcttacc taaacgactt
     2461 gctggtaaag cagaaagttc caaagaagaa aacgcataaa gagaaagacc caaagcaaaa
     2521 gcaattcctt tggctgttgc taagccaatt cttagtccgg taaaagaacc gggtccaatg
     2581 catacataga tttctttcag ttccctccgc tctacattgc agaattgaaa agcatagtca
     2641 attgccggca tcaaggtctc gctatgggtt attttaatgt caaaataggc ggagtaaata
     2701 atcctctttt cgtctgctaa agctatggaa ccgaaatttt gagtagtatc aagagcaagt
     2761 ttcaatttcc tttccgcatc caatcgtcat ataaattttc aaagtatttt ttatgctgtg
     2821 cttcatctgc agcaaggcgt ctgaaaaggg tagatatttc aggatcggga aattttacgc
     2881 tcatttcgga ataaagtaga aaggatgttt cttcgcgttt cataccttta ataagtatat
     2941 tttgatagct taggtctaaa gtttctggat cagctgtaat gtattcgctt attttcaaat
     3001 tgggggtttt gtgaatatct tcgtctttaa cacctgtctg gcgtagcttt tcaattatcg
     3061 ttatgtggtt ttgttccatt gcttctagtt cttttagcat ttctttctga tcctggaatt
     3121 gagcttggtt ttgcaaattc tgatagaatt taattgcttc ccgctcacgc gctatggcaa
     3181 aatccagaat ttcctcaaag tcctttcgtt gcatttctgt cctcacaaat tttcagtttt
     3241 ataaaaacca ccggggagat gtgattctcc ccggttaaac gggaacggat ttattcaatc
     3301 caaaggttca aacatatctt ttccgacgcc acattccggg caaacaaaat catcgggcag
     3361 gtcttcaaaa ggaatgttgt cgttttcggc aggatcgtaa atccatccac aagccataca
     3421 ttgatatttc tgcattttat tatctccttg agagtttagt aattttccat aaaaagttca
     3481 aaataacttt gaggatgatt gcaggaagga cagctcttag gtgcttccgt tccttcatgg
     3541 atgtaaccgc aattcaagca tttccagaag accttttctt cacgaataaa caccctttga
     3601 ttttttatat tatcatagag cttacgaaaa cgcttttcat gctctctttc cactttagct
     3661 accattctcc aggaatgggc aatatcgcta aaaccctctt cttcagccac atcggcaaag
     3721 ctgggataaa gctctgtcca ttcttcattt tctccattag ctgcatattc caaattttta
     3781 agtgtgctat ctgcagacca gccaacggga taggatgaaa taatattgat aatttcgcct
     3841 tccagaccca ttttttgcag atgtttgaaa aatatcttgg catgttcttt ttcattttcc
     3901 gcggtctctg taaagatatt ggaaatttgt tcaaagccct cattcttagc tgttttagct
     3961 gaataaaggt atctcattct ggcttgactt tctcccgcaa aggatttcat cagattttcg
     4021 gcagttctgg tttctttgtt gggcatattt tcctccttca attgcttatg ctttccataa
     4081 accgtgaaca ttgcaatatt cacgcacagc aataactttg tccattttca cgcaaaactt
     4141 tgccttaggt tcatctcctg gcttcagttc tttgcgtaaa accttttttt cggtgagcac
     4201 ttcaatacag gtaatatagt gcttttcttc catcggatga gggatacttc caacggagac
     4261 ctcaatttta tctcccaaat ccgttacaac ggggatatgt ttttctactg cggcatcaac
     4321 agtattttct ttaagtaatt gcattggttg accacagcaa actaattcgc caccgccaac
     4381 ataaattact tcaacaacat ttccgcaaat cgggcaatgc cacatttgtc ttaaagctat
     4441 catcttattc ctccttaagt taatatgcat tttcctttta tgatagattg atagttttgt
     4501 caagtaagaa ttttttaccg tggaataatc aagaattttc ttgccaaaaa aacgcttttg
     4561 gtttttatgc tttacaaagt attaaattag taaaaaaggt tggggaatga aacgccttgt
     4621 aataatgctg ttagcgcttc tgcttttagc tggatgcgtt caatacgatg aagaactatg
     4681 gattaaccgt aacggttcag gacatgcaac tattcgtgta gttcatcgtt caccttatga
     4741 aaatccggaa gaaattatgc gtaaagcagc attaccaggt atcaatttac aatcctacga
     4801 cattcagaga aaaggtccag atgttattta cactatcaat ttcaagttta aaagcattga
     4861 tgccttcaat aatgtaaacg atcaattagg cgctgctgat ttttggggga aaataacctt
     4921 aaataaagag ccaggcagaa gaattacttt caaacgcaga attgcacttg gaagccagga
     4981 acaggaactg gaagaagaag gtattgaaaa tatattgggt ttgctgcaac ccgaaaatcc
     5041 tgtttggaca tacaaagttc atcttccctg gaaaattatt tctaccaatg ctctaccgga
     5101 aaatgtggat agggaaaacg ggactgttac ctggagtttt gatacccgta aaatgtggca
     5161 taaacaggaa ttaatgactg ttgaattgca aaaggatttt ccctggtttc tcttagttat
     5221 cggggctgta attgttgtct tgctcatttt cctgattcat tggatgttcc gctttccgaa
     5281 aaaatcacat tggagggaac gactaaagca tagcgaaaat gcagaaaaaa gttaaaaaaa
     5341 gattttctct cacagataac acagatttca cagatttaat atgtatggga tgaagaggat
     5401 ttattagctt ttttatctta acattcttca ttcttcagtg tgcttcaaac caattttttc
     5461 ctactcccac atccgttttc agatggactt tttccgcata ttttgggggt agagcgtttt
     5521 ccatataggt cttaacgata ttttttgtct cttccaatgc ttcttgctga acctcaaaaa
     5581 ggagttcatc atggacttgg agaatcattt tgatgtcttc gcgtttatct atctgcttat
     5641 gaatattgag catagctatt ttaatcaggt cagctgcagt tccttgaatt ggcatattaa
     5701 ccgcaattct ttccgcttcg ctttttagtc cctgattttt actgtctata ttaggaaggt
     5761 ataatcgtct gccaaaaagg gtttccgcat aatgttgttg acgagcttta tttacacatt
     5821 ggttgataaa attacgaatg gaagggaatt gggcatagta accgtctata atgtcttttg
     5881 cttcattgag agagattccc agttcgcggg aaagtttgcg ttgtcccatt ccgtagagaa
     5941 tgccaaagtt aatggttttt gcagctcttc tttggtcggg agtaaccttt tcaatgtcca
     6001 cattaaaaat tttggcagcc attcttttat gaatatccag gtcttgttta aaagcatcta
     6061 ttaagacctc atcttcgctc atcagagcca gcaaacgcaa ttctatctgt gaataatctg
     6121 catccaggat caggtgttct gcatctttgg cacaaaaagc tttccggatt tcgcgtccaa
     6181 tttctgtgcg cacgggtata ttttgcagat tgggattgga agaagaaagg cgacctgtgg
     6241 aagtaacggt ttgattaaaa gaggaatgta ttcttccggt ttgaggattg atcattttag
     6301 gcagagcgct tacataagta gattgcagtt ttgaaagctg tctatattga ataattattc
     6361 cggcaatttc atagtcaatg gcaagttctt ccagcacact gttatccgta gaatagccgg
     6421 ttttggtttt tctaccgtag ggtagttttt tttcttcaaa gagcagtttt gccaattgct
     6481 gggttgaatt gatgttaaat tcatatcccg caaacgcata tattttatct gtcagggtct
     6541 tcaattccag atttatctga tgggatattt cgcttaaaac agaacagtca attgccactc
     6601 cgttcatttc catctgttgt aagaccttga taagggggag ttcaatcgta tcaaaaaggg
     6661 ttgccataac gctaaaatct acttttcttc ggtaaacagg atagagttta taaacagccc
     6721 aggcatcttc tgcagagtat atacatgctt tttccggttc taccaggtca aaagtgctct
     6781 ggtttttacc tttgccaatt aagtcactaa taggtatcat tttataattc aattcattca
     6841 gagcgcaggt ttccagagag taattcagtg ttccaggatc aagaatataa gcagccagca
     6901 ttgtatcaaa cagtggatta tcaaggtcta atccattgcg ggcaagaact attaaatcaa
     6961 acttgagatt atgtcccaat aaaagtttcc cttttaaagc atcttccaaa tggcgaagaa
     7021 cttccggcag cggcaaattt actgcaagtt gatgtcccaa tggaagatag tatgctttct
     7081 tttcatccat acaaaaggat attcccacca aatttgcccg cataggttcg ggggaatccg
     7141 tttccgtatc aagagaaatt tgttttgcct ctttcagttc tttgagcaga gtaacaaaat
     7201 tgagagtgtt aaccaaaacg gctgaaaaag gcattttttc ctctgtttct gctgtgtcct
     7261 cttgtggctt ttccttttcc ttaaaaatat cgtcctgata ttcggtttgt tgttttgttg
     7321 cattggtttc cggatatttc agctctattt tccgcttaat ggaattaatt tcatattcct
     7381 ccagcaaagg aattgctttt ctcaaatcct ttgcccaaaa agttaggtgc tctaaatcgg
     7441 gtttgggaat cggaacatcg gtgtcaattt ttgctaattg ccttgagaga taagcatttt
     7501 ccttattttc ccgaaggact tcctgcaagc gtggttttaa cttatccaaa ttagcataga
     7561 tattatccag cgttttatat tcggaaagta gcattgcagc agttttaggt ccgatgctgc
     7621 tcacgccagg aatgttgtca gaagggtctc caacaagcgc aagataatca ataaactgct
     7681 ccggataaac accatatttg gcatatacgg catcccgatc caaaacaata tctttcatcg
     7741 gatcaagcat aactgaacgc tcatcaacca attgacagta atccttgtca ctggttaaaa
     7801 acacaatttg aaactgatct ttatattgct ttgccaaagt ccccagaata tcatccgctt
     7861 cataaccatc aagaccgatt tcctttaagc cgattaactc aaaaaatcgt ttcacgggtt
     7921 caatttgagc cgtaagttct tctggcatag gagggcgctg agctttgtat gcctcataaa
     7981 gattatgccg aaaagtagga gctttacggt caaaagatat ggctacataa gccgcttgtg
     8041 tattttccag gatgtgcaga aacgaattta ttactccgaa aatagcactg gtatgcattc
     8101 ccttgctgtt gattaaagga ttcttaatga aggcaaaatg agccctgtat aacaatgccg
     8161 tgccatcaat taaatataat ttatcagcca tttcctaacc gtcctttggc ttgattttct
     8221 tacttgataa cacaataggg aacgggattt tttggcaagg gaattctgca aagcgcgata
     8281 aagaattaga atgaaaaaag ttgagaggat tgataaggtt gagatgattt agagagttta
     8341 gaaggtttat aaggttgaga tggtttagag agtttagagg gttgagaagg tttataaggt
     8401 tgagatttca taaaagctcg tcaggacgac acaatgatag cgatgggtgt gtaaacccct
     8461 cgtccaattc acgattttca gcagagaaag ttttgactca caattttcac ctgccttcca
     8521 gaaagccaca atctacaaac tatttcacgt acaaccgcat tttaactggt ttccaaattg
     8581 aaaaattgcg agttaaaaca gaaagcaagc acacttacaa ccgagctata acaatctcac
     8641 aaattatacg gtaatgaaag aatctatatt tacacgataa aaggaaaagc aagaataccc
     8701 tttttaaaat tatatcattt acgaatgaac aattatattc tttacaatgt attatactgt
     8761 ggataccaat aaaaacaacc tgatcatcct gtatctgctt ttgaatttta tttataagaa
     8821 taatagagtt aaatccgttc ctggatgtta aataattata ttttgcctat ccaaatagct
     8881 atcctatcta ttatattgta tttcttattt tggtaaaata ttcttatttt ccctattatt
     8941 ttcagggtta aaacccttct ataaaatctg tcgttcctac ggaacttttt cttaaaaaaa
     9001 gagataggta gtttttctta cccttttcgc cgggttaaaa cccgtcgtta gtatctgtcg
     9061 ttcctacgga acttttttcc tattatttat tcttattgaa tataactatt gtataaaata
     9121 tgaacttacc aatacaaaat ttcattcttc tcaataaatt ccctaactcc tactaaataa
     9181 atataattcc gttttcttcc ttagtagcat tgcttactca ttgcttaccc attgctaata
     9241 ccatccgtaa tgggtaagca atgcttcagt aattcaagtt ggaaaaagct gaaataccct
     9301 gaaagcagtg tactttattt aaccccaaaa atgtagttta taacaaatgg attggcaact
     9361 tctactttgc caaggagttc gcagaaattt gaaaaataaa tgcattcggt ttattgcaaa
     9421 ttagaatacc gcaactaaaa tacaaaattt gtcttttctt ctccaggtta aaacccttct
     9481 ataaaatctg tcgttcctac ggaactcatt ctcccagaag gaaataagct attttctatg
     9541 ctcttttcgc cgggttaaaa cccgtcgtta gtatctgtcg ttactacgga actttttccc
     9601 atatatgagc ttctgctttt atagaaattc cgattaactt gtaaatctga aaacgctatt
     9661 ttatatatac tgttcccatt atcctttttc aaaaaaatca aagaattctg ttaatttatt
     9721 tatccagaga ttatatttct gatagtatga attatattct tcatcagtaa cgatattgca
     9781 tttggaaaca acataaaagg atggagttcc ttttaatttg actactatat tcactggatt
     9841 ttgatcatta ccaagataat atttcccaat taaatagacg gttctccaat tattcatatc
     9901 aagtggatta tcccattttt ttatttttga acctggaatg gcaaattcag ctgtttgtaa
     9961 cagatcaata taagccataa atgcttgagg atttttagaa taattttgtt tgaatttatg
    10021 aggtttgtat tcataatcat ctaaccagta atcttcaaaa caaattaaat taggtaaagc
    10081 aggacaacgc aagtatgttt gataaacttt tacagaaagg aagtttttaa cctgtgtctc
    10141 tttaatttta gggacccgag gataaatatt ttcatctttg ggtaaactgg aaaaagcaat
    10201 ttgtagcatt ggagtaatat aatcaaggac attaaattta tatgcttttt gaattgcatc
    10261 attcttccag attagttttc cagatgctgt ataaatctcg caagcaaagc tataagtata
    10321 agtatcatat tgattggtgg aaacttcact tttagagacc tgtatagttt cggaaataag
    10381 gtttgccaaa gttataccat aaccactttt aacggtcatc gccgatccgg aataattaga
    10441 attttgaatc attgatgtag taatagtttc cagctgtttt tcaacggaaa ataaaacttt
    10501 catcttgtat tgataattgc tgtttgaaat ataaaaacct cttctttcaa gaagtgattt
    10561 ggctttttgc gctatttcct cttccaataa ttcttgggaa ccaattaagg gtgtggataa
    10621 aatagttgtt tcaatggcaa ttgtagagcc ctgaattatt tcctcatctg ataaagtttt
    10681 gttccaagga tggggtacta ctttgggaac tgtggaacaa cccataatga gtaaaatgta
    10741 tagcataaca taaagatagc gtttcatttt tataatctcc tcaatccatt ttttttcgtt
    10801 gaatcttttt ctctaatggt gatgctgaaa agatatcaat tttatgaata attatttcat
    10861 cttgaaaatg ctttaatttg tcaagagaat tccggctgaa tctttggagg tagttttaac
    10921 tcacaatttt caactgcttt cccaattata aacaatcctt ttgctttttc acttccaacc
    10981 gcgctataaa tggtttccca attaaaaaat tgtgagttaa aacagaaaat cgttatatct
    11041 ttcactttac gaggggctta agtacacatc gctataattg tttcgccctt cgtttttttt
    11101 agaatgagtt acagcaaagc tattgacaga aagcagggtg gttaaaaaat tgctaaatgt
    11161 taatataaag aggaaaaaag agtggaaaga ccttcctggc aacagtattt tatggaaatg
    11221 gcatatcttg cttccaagcg tagcacttgt ttacgcagaa aagttggagc tgttctcatt
    11281 cgtgacaatc aaattatagc tactggttat aacggcagtc ctaaaggagt tccccattgt
    11341 gaagatattg gttgtttaag agaacagcaa aaagttcctt cgggcaaaaa tcaagaactt
    11401 tgccgaggag ttcatgctga acaaaatgct ataattcagg cagctatcaa tggttcttct
    11461 actcggggtg ccattcttta ctgtaccaat cagccctgca gtatctgtgc ccgtttaatt
    11521 attaatgccg aaattaagac cgtttatata gctgaatcct atcctgatga attaggggaa
    11581 gaactcttta gagaagcagg tgttgaactt atcaagtttg atttgcagag taatacctta
    11641 aagaaactaa tctgatgagt attttccggc gttttacggt aaaggactta attataatca
    11701 cttccatagc cgctttggga attgccataa aaccgattat caatcctgta accaagttat
    11761 tttctacacc tttgggaata cctgggggtt caattaccgg tggactttat atgttatggt
    11821 tagttttagc ggttgtgata gtagataaac ccttcaccgg aacgctttat ggtcttttac
    11881 agtcaatttt ggtattactt atcggaatgg caggaaaaat gggtgctttt tctttagtgg
    11941 catatactgc accgggaatt gttgcagacc tattattttg gcttctgccc tataaggata
    12001 agctgataac tcatttgttt ctatgcggtt gtgccaattt aaccggctct cttatttctt
    12061 ccgctatttt ttttcagatg cctactgtga tgatagtttt caatacttgt atggcattta
    12121 gttccggtat tttaggcggc tatcttggtt ttggcattta tcaatcgctg aaagcagcaa
    12181 ggattattca atgaagaaat atttgtgttt tttactctta tcgggagtta tgatgactct
    12241 atcgtctttg gaagtaatag atacaaaagg cattagccat ttttatagta atgctgaact
    12301 ttataaaaag gaaacgcagg aaataaaaac ctctcgcgaa aaagatggaa ctgtgcgtct
    12361 taacacctgg cagggttttc gctttgatat ttggctaaaa gagcaaaact taggtgactt
    12421 tgctaccatc cgttttgaat cctcagaccg ctatcaagta accctggaaa aaagcgaatt
    12481 tgattctttg gaaagttact tggttacggg tcaggatgga attccttttg aggaaaatat
    12541 gctgcgctta atttttcctg ctttaagaga aatgcaatgg ataaagggtt tggagcgtgt
    12601 tgtgctggag gattttcaac cgctaattcg tccccagcgt ttttatttgc tgcaggaaac
    12661 cctgaaaaaa tatcctttac agcaagaccc cgagcccttt gtaaaaacac agggttggta
    12721 tctcagggat attttgtcgg atttatcgga agcggaagaa aaacaggtaa ttttatacag
    12781 tagagatggc ttaaaacagc atttgaccta tcctttacat ttgtcagggg caattctgga
    12841 aaaaacagct gaacagcatt acaacttaaa aagtccgcaa attccaggtg gaatgtggat
    12901 gaaagatgtt atttatctgc aatgcgataa taacgctcta attagtgagg actctatcaa
    12961 tcagctgatt cgtctggcaa aaatattgca ttgggagacc acaccggaaa tgcaattcag
    13021 gattgttttt gaagataggg aagaggtgat gaattttaca gatgccttgg ctgaaccgca
    13081 ggtctttagg ggagcgcttt acttagagct tttctgatgc tttttttaca ggatttcggc
    13141 atttcttttg cagaacagaa attatgggaa ggtatcaatc taaaaattcc tgagggctct
    13201 atatttcatt taaaaggacc taatgccagc gggaaaagtt ccttgttgaa tgccattagc
    13261 ggtataattc cggaatatgt gaaagccaaa actacaggga acattctttt ggggcaaacg
    13321 gatttacaag caattccctt aaaggagaaa tttcattatc tatggcatca gttaagcgat
    13381 gcggaagcac agctgttttt tccggattgc ctatccgaac ttgcttttgc tttggaacat
    13441 cttgccttgc ctgcggaaaa tatcgttgct aaaataaacc tggcagcagc acaatttgga
    13501 ctggaaaaga tgttacaacg agacccttca actcttagcg gaggcgaaaa aaaacttttg
    13561 ctttgtgctg ttgcggaaac aattgatcct cctttactgc ttttggatga acccttaaac
    13621 ggattggata aacaagcaac cgatttggtc tttaagtggc tgaaaaacaa gaaagagcaa
    13681 ggtaaaataa tagtagtagc tgatcacaat ccgttaatgg aaacagtagc tgatttttcc
    13741 tatactttac cggaaaatcc gcaaccgctg actatagata attcattatt taccgaggca
    13801 gttctgaatt tgcaggataa gcgggattgt gctataaaac ctattcctga tgttctttac
    13861 aatattgaag aactcagttt tgcttatcca aatgcggaat ttttgtttaa agacctctct
    13921 attaagataa acagcacgga aaatattcta ctgaaaggga aaaatggagc cgggaaaagc
    13981 actttcctta aattgttaac tggattgcta aagcccaata aggggaaaat atacttgcag
    14041 ggaaagcttc tgcagggttt aaaccccaaa aactttgctc acttttatta ccaaagccag
    14101 attaccaagg aaaacctttt agggataagc gttaaacaaa actggctatt ctggcaactg
    14161 gcaattccgg aactaaagga tttacctata aaagaagacc ctttgtttac agagctttct
    14221 gccggacagc agaaaatggt ttcgcaacaa attttaccct atatgttatc taaattctgg
    14281 atattggatg agccctttgc ttctttagat gaaaatgccg gcaagcaact gtttaattta
    14341 ctgcaatata aaagccgaaa ctatccggga atgctgattg tttcacattc tttggaagcg
    14401 catagggaac tttttgatag agtgttctct ttccaaaatc aaactttgca ggaagaaaag
    14461 ggatgaaaaa gagttctttg catcctctaa ctcatatctt gatagctttg ttgctttcca
    14521 ctttcgtttt tatcgccaaa aagccagttt ctttgctatg ccttactatt ttggctttgc
    14581 tttatgctgt atggaggatg gaaaacggct ggcaaaaagt attcaagaca ctttggcata
    14641 ccttgccctt tttattggtt ttagctttct ttcaaattgt tttccgacgc acaggaacat
    14701 tactttggtc ttatggaata ctaaaaatca gcgaggaggg agttcttatt tctctacagc
    14761 taactttgcg tttattaacc gttatttatt gtgccaaggc attagcagta atggattata
    14821 cggaattttc caaagcattt tcggctattc gtttacccga agagctatcc tttatgcttt
    14881 cttatgctat tcatttggta ccgaagttct tagggcagtt aaaaggattt gttaccgggc
    14941 ttaaattgcg aggaattgat gctgctggtt tatccttttc caaacgctta caggtttata
    15001 aacaattgtc cattacagct ttggcagaac tgatcagaag tagtgaaaca gcagctattg
    15061 ctttggaatt aagaggtttt agaagtgaag gaaaacgaag taaactgcat cggcagaagt
    15121 ttagctacag ggatttattg tgtttgctgt tcttttcggt tttggttatt atagttacaa
    15181 ggatttgatg tagcacgcag attttatttt ttcacaaaga gaaaaagagg gtattttttt
    15241 tcacaaagag gaaaagagga aaagagaaaa gagggaattt tttcacaaag aggaaaagag
    15301 aaaaagaggg tattttttac aaagaggaaa gaggaaaaga gattttttta caatgagaac
    15361 accgaaaaaa ctgagaacac ctctttcttt gttaatattc ttttttttcc tctttatctc
    15421 tttgttaaaa ttatcttttt tcctctttat ctctttgttc aaattatctc ttttcctctt
    15481 tatctctttg ttagaattat ctctttatct ctttgttcaa attatctttt tttcctcttt
    15541 atctctttat ctctttctct ctttgttcta tctatttttt tgcactggcg atcacaggtt
    15601 tgggatgcat caaattttcc atatcctgca gaattatctg ttgctggctt ttgaaccttg
    15661 ccatctgcgt ttcattcagc ttttccgttc ctaccatttt caggtttgta ggattgatat
    15721 actttttacc tttgattaaa ccaaaatgca gatgagcacc tgtggaatgt cctgtagagc
    15781 caacgcgtcc tataatttgt ccttttttaa cggtctgtcc tttgcggaca ctcattgaag
    15841 aaagatgtgc atattgagtt gtaagtccac tgggatgttt tatccgaatt tcatttcccc
    15901 agccgccgtt ataacctgca tccgtaactt ttccattggc tacggcataa acaggagttc
    15961 cataatgcgc acgataatca gctccctgat gattagccca gtttccataa aaaggatcaa
    16021 ggcgtctgcc aaaaggagaa acaagatgaa ttacagataa aggaaaacct actccgttgc
    16081 tgtggcagct ttttccttct ttagtataaa gaccgtttag cactgatttt tcttcctcat
    16141 cttcataacg gaaaagttca caagtgccgg ttcgttcacc gctatattga acataaaata
    16201 tttttctgcc gggcagggtt ttcccttcaa aaatgcgttc ttctacaaag acgcggaaaa
    16261 aatcaccgtt acgcgcatca cgggcaaaat ttatttctgt ttccaagccg ttatttattg
    16321 cttgtttatc ctgaggagca aaacccatag ctaatagaga agcatccaag gtcccttcta
    16381 attttccttc caggatgcgt ttaactcttt taacgggtaa tgcctccaag gaatagacaa
    16441 gagaatcggc actaaccgta aaatgatgac gggtagttgg ttcctgcaca aacatcattt
    16501 tagcaatgtg ttgtttatct tccgaaagca atattttcag cgtatcccca ggttgaatag
    16561 tggatacatc aatatagtca ccgaatttaa aagccagcaa gccaacttct ttcaagggta
    16621 aattcaaatt tgccagcacg gaaaaaatac ttcctccctc aggaatggtt tgtattatcc
    16681 agggttctga gggtttttgt aaatcgggct taatttctgc aggatgttta ggattgatac
    16741 ccattaaact gataagacaa aaagtagtta aaagcagaaa aataaaggcg agaagaatgt
    16801 cttttcccca attcataagt taactccaat gtttgtttta gagcccgggt cggggtctgc
    16861 agaagagtgt ttttcaaagt aaggatggaa gtgaatatgc acttgtctta agccggtgct
    16921 gaatttatcc agcaggcgtt tttctacaga atcggaaata atattgcctt tatcaacgct
    16981 gatatctcca tccactgtaa tagtcatatt cactgtaaaa tagggaccga aacgatgaat
    17041 ccccaggtct tcaatactac gaactccttc cacagccatt gcttcggttt tcagttcacg
    17101 cacaaaatcc tcgtcaggaa gataatccat cagttctctt gaggattcca ttattatttc
    17161 tactccggtt tttatgatat aaatagcaac tatagctccg gcagccgggt ccatccaata
    17221 atagccaaga cgacccaaaa ggacaccaac tacaacagct acggcagcca taatatcatt
    17281 cagatggtca ttagccaatg ccttgagagt aggatttttg gttttgggta tgtttttgcg
    17341 agtgtaagta tagaggaaaa ttttcaagcc aaaggtaaag agcgctataa ctaaagtcca
    17401 aatagaaagg ggttgtccaa tttccgtttt ggaaagcaaa tcatagacct tatttaccga
    17461 ttcccaaaaa atggctattc cggtagtaag aataaacgcc ccaaccacaa ttgcagaaat
    17521 agattccagt tgtctgtgac cataaggatg ttctacatcg gcaggttttt gggcttgttt
    17581 cataaagatt ttaacggcaa tgtaatagac aacatcggaa gtggaattta taccatcggc
    17641 taaaagagcg gcactatgac ctaaaatgcc tacgcaggtc ttgacaatag ataataaaat
    17701 attggaaaac agacctaaat ttacagttac tcgggttaaa ttatcgcggt ccgtcactat
    17761 tttatatctc catcacatcc aacagaggat attctttaaa agttctgcgt aagttctgat
    17821 gaaattcatc ggcatcaata tcgctgttac caattccctt aggattaagc gctattgcct
    17881 tcagcttgaa aggaattaag gcaagggggt tatgattttt taaaaagaca tcaagttcat
    17941 tactactaag tttgatacat tccgggtgac gaaagataag ttgaattccg cttaggtgtt
    18001 ttcctaaatg attattcaca ctgcttgaat aggcaccggg aagatagaga tggcttggtt
    18061 tttccagttt acttaaaatt tccagcaact tggtttcgtt ggcaattaaa gacaccaaac
    18121 ccgtttgttt ccatcttcct ttgtgtttta tcaatatctt attttgttcg gcaagttttt
    18181 gtaaggtggg gcttaagtat gttccaatct gagataaagt tatcagtcgt tttagttctt
    18241 caattattgc ctgctgatta ccaaaactgg ctccggcagt taaaattatg gcatcaacgg
    18301 cattgcttaa aacaatggat ttacggtcca gagagccatc tattagaact ttttcagctc
    18361 ccattttttg cagtaattca gcgcaggcaa tttgtccact aacgcttgcg ggaccggtta
    18421 tttctgtttc cagatatttt tcgcttttta ctatccagag ttctctgctt ccgctttgga
    18481 aagaggtttt agtcagaatg cttattcctg agccgtgttg caacagggtt ttagcatcgg
    18541 tacaaaaaaa acagccctca ggaattttca ctttgggctt gggaatttta aacaaagtat
    18601 cttccgtttc accatcgcgt cctgtactca aaatacccca cggaaaagga tattttgcca
    18661 gaatatagtt caggacagtt gtcttacctg catttttaca gattcccaca atcgctatat
    18721 ttgtgtaacg cctgccggaa atccaggaga attccaaaaa gtccccttta atgttttcta
    18781 cgacgattac gcttcgtttc tgcgggacct atggctactt ttttaccccg cattaacgcc
    18841 attatacctt ctgtggattt atattcatcc gcacaaagtt cacggtattt gcttttatcc
    18901 tgttccacaa attccggttc cgtgtatgcc gtaataaaac cttcataatt tcgtaaaata
    18961 actcttcccg gcatttggga aataacataa ttgggcatca caggaatttt tcctccacca
    19021 ccaggagcat ctaccacata agttggaaca cataatccgg aggtatgacc tctcaaactt
    19081 tccataattt caatcccttt ggcaataggg gtgcgaaaat ggctgatacc ttcggataaa
    19141 tcacattgat atatatagta agggcgaact ctatttttca ccagtttatg caccagggct
    19201 ttcattacat ctacattgtc gtttacacct ttcagtaaca cggattgatt gcccattgga
    19261 attcctgttt cagcaatttt ggcaagcgct ttacaggagt cctcacaaag ttcattggga
    19321 tgattataat gcgtattcag ccaaacaaac ttatatttct gcaaaatttt caacagtgaa
    19381 tccgtaaagc gttggggcaa aacaaccgga gttctggtgc ccaaacgcac aatttctata
    19441 tgttcaatct tacttaatct gtttaaaata tcatccaaac gctcgtcact taaagtgagg
    19501 ggatcacctc cgcttaagat aacatcgcgc acttctttgt gttcttttat atattccaat
    19561 gccttttcca cattttcttt gggcatagga gcatcgtgtt caccggcttt tctccggcgg
    19621 gtacaatgcc tgcaatacat tgcacattgg tcagttagaa gaagcaaaac ccgatcagga
    19681 tagcgatgtg tcattccagg aacaggagca tcggcatctt cacttaaagg gtctgccata
    19741 tcggatggcg aaatatggct ttccgcaatg cgcggaattg cctgtaaacg gatcggatca
    19801 taaggattat tgtgatcaat caaagaaaga taatgaggag tgatagccat ccggaaagaa
    19861 aatgccttgt ccttaaaaac tgcttcttct tcgggttgca gttcaatgta tttagatagt
    19921 tgagcgtgag ttgtgatgcg atttttaatt tgccaatgcc agtcattcca ttgttcagga
    19981 gtggcaatgc ttttaatgtc aattagtttg ctcattttgt cctctttatt tattccgcat
    20041 agcgttcttt aaatatcttc attaaaactg gattgcgccg taataaatcc aaagcaattt
    20101 cggaatgatg atgggcataa ccattaccaa tcatcatatc tacatcggca ccaattcctt
    20161 ctgcgcccaa agctgccttt gtaaaagaag tagccataga gaaaaaatag accaaaccgc
    20221 gatctttggc tgccataata ctcggcaatt cagtatcttc agtatttaca acattgataa
    20281 ctacatccgc cattttgccg ttagtaaggc gcgagacctc tttttgaata gtgatagcat
    20341 ccgtagcttg agcaaggata acttcatcac aaccggttgc tttgcaagtg ggaatataat
    20401 tttcattgcg caccgctccg atcacttttc caccaattcc tgccctttct ttcgctacgg
    20461 cattgcataa aataccgctt ttaccgttag caccaataat aacaactgta tcatcaggtt
    20521 ttaccagtcg ctctacttgt gcaggagctc cggcaacatc taaaacggaa agagccagat
    20581 tttcatccag atcatccggt aacttggcat aaacgccact gctgaaaaga attgcttgac
    20641 cttcaatttc catttgatcc ttgtctaaca gcactttttt cactttattt attttgagag
    20701 gagttagcga aagcgaaacc agagaagcaa ttttatcccc cactttcact tcttccttca
    20761 tctcaaattt atcacctata gcggcaaccc tgccgataaa cattccgccg gaacccgtat
    20821 cttcattttt atgcttgccg gttctgttgg taagttcaat agcgtgttcc gcaaatgcct
    20881 tttccaaatc ctgatgtcct tgggcaataa gcttgttttt aatctgatga aaggaagcgg
    20941 aatcaatatt taaacgaata acatcaatca gcatttcatt gtcccaaatt tcgctcatat
    21001 cgttattcaa tatttttgcg ggttgcggta aaacaccttt tggctcaatt acccggtgcg
    21061 ttccatatct gcatccatta cttttcattt gcttctcctt tctgaatgtc tatcttatta
    21121 tttaatttca aattagctat aaaaatccac ttttttatat ttgctcttgc tgtcaattct
    21181 tttatataag taagctcttt ctataattct ctccaaaaca ggttattttc tttagagagt
    21241 tcctttccta aagcttttga ataacacgat atttgctttt cttatattgt cccgtgcttg
    21301 ccaatttttt acttgttcac tgccgatata attccgatat gattcccata tcgttcccat
    21361 atcacgatat gggaagcata agggatctta ttgaaaagta tagcgttagc cgtcaagaaa
    21421 agaacttatg ataaaacata gatagaaaaa gcgaaatgcc agctctacaa attttagctt
    21481 gctaattaaa aaacccgcag ggcagcacaa tgatagatat ggtagtgtaa accccttgta
    21541 aaatgaaaga tataaaactt taaaacctta tctaaaactt tgggagagcc ccgagcgcaa
    21601 cacttctatt tagacggatt tttggctctg gggaaaattg tagttgggaa attacagaaa
    21661 aactgcaaaa ttttgcttga cattttcata tctctaataa tattatcttt agaatgcaga
    21721 aaaaatgcaa atctggagaa taaaatgatt attactttca gtgtccaaaa cttcttttcc
    21781 atccggaata aagtaacttt ggattttcgt gccaccagtg acaaaaccct tgaggactat
    21841 tatgtagttt cggttgaaaa accaaagcta agaattctta gaatggctat gatctatgga
    21901 gctaatgcct ccggaaaaac taatctgctt atggctttgg attttataaa acgatttatt
    21961 agtgatacat ctataaacaa agaaattcct attccaatag ccccttttgc tttggatgaa
    22021 gataagtcat ctatctttga gattgatttt cttcagaagg gaatcaagta tagctactat
    22081 ctggaattga acaaaaaaaa tattattcag gaagagctaa gctattatcc aaagggaaga
    22141 aagaacctta tcttttcccg caaattagtt gacatcaaag gttttatata cgaatatgaa
    22201 tggagaaatg cagcaataaa tgaaaaacaa aaagacgctt tggaattaac tgtccaaaat
    22261 caatcaattc tttctaaaat agcgacaata caatattcgg gtgttctcca agaagccaga
    22321 aactggttta ctataactct aaaaaatatg ttgggttcta aaacagtact tttagggttt
    22381 aactatttta attatcttaa tactcccgaa aaagaaaaac gctataaacc attatacata
    22441 gaactattaa aaagagctga ttttttcatt gaagatatca cttttgaaga aaaaatattc
    22501 actatggctg aactaccccc tgaggttaga gctaaatttg aaactaatcc taaagagcaa
    22561 gaagatgata aagaacaaga agaaatatca tctctaaaaa ctgaatttat tcataaaact
    22621 ccatcagaaa cttatgtgtt aaattaccat atggaatcaa tgggcactca aagatatttt
    22681 ggtttgattg gaattctttt agagcttatt tacaaaaatc ggattgttcc tatagatgaa
    22741 attgaaagtt cattgcatat tgatcttatc attcattata ttaccacttt tttaagaaat
    22801 aagcgcaagg gacaattaat tttcacctct caaaatacgg ctctcttaaa agaaaaagat
    22861 attttacgca gagatgccat ttggattaca gaaagaaatg aagatgggag tacctcactt
    22921 accagcgttt ctgaatatcc cgttcggaaa gaacattcta ttgaaagtat atatagaaaa
    22981 ggattaatcg gcggtttacc caatctggga actactctct tggaaggtga aaatgaaacc
    23041 aaggacgaat aaaccgacaa aaccattcaa aaatacggta ttatgtttct gtgaaggtga
    23101 aacggaaaag gtatatttaa cttgtcttaa aaaagataga tacaagaatg tgcacataga
    23161 gctgaaaccc cagatcagca atgccgattt tgaaaagacc tttaaggata ttaaacacca
    23221 acttgcaaaa cccaaaaaaa caaattataa atatatattt tatgtgttgg atatggatgc
    23281 gatttataac caaaaccaaa ttgatgaata caatcgtcaa aaagaaagaa ttaagtcgct
    23341 ggttcaggca aaagaaagat taacaataat tgaatccaga ccctgtatag agttttggtt
    23401 ccttttacac tataaaaata cggataaatg ttttgtcaac tgtgatgaaa ttattgttga
    23461 attatgtaaa tatattccgg aatattgtaa aaatcagaat tatatcgcct ctttatacga
    23521 aaaactgaaa gataaattgg aaactgcaag gcaaaactca gaagcaatat gtaaaaaaga
    23581 aagagtagat aatgaagatt attcctacac tcaaatgcac caattaattc aaattttgga
    23641 tgatttgaaa aaataatatc tcccattatt ttaaaaggaa gaaaattcta attctacatc
    23701 tttacttgac aaatcaagag gggaaattaa aaagaaaaat agtgattaac ttattgggag
    23761 ccaaataaat ggaaaggggt attaatttta tattaatggt aagaggataa aaggcaatct
    23821 atgacaaaaa caggtaccga aaccagtcct tttggaacca aaggaagaat taatcacgat
    23881 tcttccaagt tctataattc caaattatat gaagaattac cgatccagaa agctaatgat
    23941 aaaacggaaa atcctttccc ggaagaatat ttgaataagg ttattctcgg ttcggcagaa
    24001 aatatgcacg aaattcctga taattctgtt caattaatga ttacttcgcc tccctataat
    24061 gtcagcaaag attatgatca gaatttatcg ttaaaacaat atctgcaatt attggaaaat
    24121 gcttttaaag agacctacag agttttagtt aatggtggaa gagcttgtat caatgtcgct
    24181 aatgttggca gaaaacccta tatccctctt tcagattata tttctcaaat tatgattaag
    24241 attggattca atatgagggg tgaaataatc tggaataaag ccgcaagtgc aagtccgtca
    24301 actgcctggg gaagttggtt gagtgctgcc aatcccattt taagagatat tcatgaatac
    24361 attcttgttt tttccaaggg tgatttctat cggtataaaa agaatagaca aaacactata
    24421 agtaaagagg actttattca gtggacaaaa tccgtgtggg ttatgaaagc cgaatcggca
    24481 aaaaaaatag gccaccctgc tccttttcct gaagaattgc cctatcgctt aattcaactt
    24541 tattccttta aggatgacat tgttcttgat ccttttatgg gtagcggaac aactgctata
    24601 gcagctttaa aatcaaaacg atattttacc gggtatgaaa ttgagcccgc ttatattgat
    24661 ttatgcaaca aacgcattag tgcttatctt gatcagctaa gcattgatct ggaataaaat
    24721 aatatcatca ttatattgat agatcataaa tggcagaaaa agttcctacg gaactttttc
    24781 tcgggaaaag agataatggc tctcctgctt tcttttcacc gggttgaaac acgttgttaa
    24841 tacctgtcgt tcatacggaa ctttgatgaa taaatggatt cctactggtg caggaaaagc
    24901 aaggagctgt caatttgaaa taatattaga ttgttacttg agctgatgag ctcatatatt
    24961 ttaacaatat aatatattaa ccataagggg aaaaatatgg tttattactt caagctgacg
    25021 agctcatata ttttaactgc gatatttttt cctttaacct tcacttcatc caggtaattg
    25081 gcatttacca ggtctttaac tttttcataa gtgaactcac tgatgatgat atgattgaaa
    25141 gtgttatatt ccttatttag tccttccaat cgtgctccca aattgattgt atcgccaata
    25201 gcagtataat caaagatttg ttcgctaccc agatttccca caacagccat tccggtatta
    25261 ataccgatac ctatttcaaa gggttcaatg ccttcgcttt gccatttttc ctgtaagcgg
    25321 gaaagacatt ctctcatttg caaagctgtt ttacaggcat caaaagaatg attaggcaaa
    25381 ggaacgggtg ttccataaag agccataatt tcatccccta caaatttatc cagaataccg
    25441 tgattgttga ttattatttt aaccatttcg gtaaggtatt cgtggaggat attgactgtt
    25501 tcctgaggag agtgtttttc ggaataagta gtaaaattgc ggatatcgga aaacaggaca
    25561 gttatttcct gtaaagaacc gccgaatttt aagcttttag gattggcaag aagttgatta
    25621 acgagttccg gagcaagata ttgctgaaaa gcatttctga taaagcgttt ttctttcatc
    25681 gttacccaat agtgatttaa gatgctggca acataaataa gaatcaaaag gataatcgtt
    25741 tgtgcgatgg gacaaataat tccagctttg ttaaacagca acagattgat aataaataaa
    25801 ataatcagca gtccaagaag agctaaagcg gaaaactggg gttttaacac tttaaaaaga
    25861 aaccaaagcc caatcagcaa aacaacttca atcagcaaaa acagccaggg attaagtttc
    25921 ttcaagtaat ttcccgttaa caccatatcc agaaaattgg cttgaatttc tactcccgaa
    25981 gtccattctc cgccataagg agtgggaaat ttatcgtgca attcatcaat tgtagctcca
    26041 accaacacaa ttttatcttt taaaacacca ctatctctta tttcgtaaaa ttcatccaat
    26101 tctgcaccct gataaccagg cataaaaaaa gttgaatcat ccaaaatgct ggcataagaa
    26161 aaagcaggat aagttcctgc aggaccccgg aaattgatga ttgccttgtt tttatctgca
    26221 aggggaatac ttttatcggt taaatgcaat gccttgctgt taactttaat gtgctcagcc
    26281 caattgtttt gatagaccct taaatttgcc agagatgcaa ttcctatgct atagagtttg
    26341 gtttgatcaa agttttcaaa aagggtgtat ttacgaataa aaccatccgg atcggcttcc
    26401 atatttacaa ttccccaagg caaattatat tctgttatgg gactaatggg aggaataatc
    26461 tgactgggtt ctccgggacg ctgtgaagtt aagacctttc cggcaaaaat tacattctga
    26521 tatctatttg cagtttctgc cagccgtaaa tcactttccg gcaaagtttt ttccgtaaac
    26581 tcaatatcaa atataatctg cttggctccg gctttattca gattttcaat taatttggaa
    26641 tgatagtccc ggggaaaggg ccaggtttca tttaaagcgc taaaggtggc atcatcaata
    26701 cctataataa caattttatc ggaaaccggt aatttacctc tcattcggaa aagcatatcc
    26761 tgagctttgt attccagatt cttaaagaaa ggaaaagcaa agataatttc tacgattagc
    26821 aggattataa cgaggtaaat aattactttt tttacgggca tctaaacctc ctgaataaaa
    26881 agcccgtctt gcagaaaaat aactttctgc aaaacggaag caataaattg taagtcacta
    26941 ttcaaattag aaccgctgtg aaagcagaac tctccaaata aaattactgt aatcaccagg
    27001 ggtgttatct ttattattaa aatcggttaa agcgatatta gtatttacgc ttaaggactt
    27061 tatagggtat gcttcaaccc ctaaagtgta atattgataa cttctatcac cttgagctcc
    27121 actggcagaa acaatccgat aattggtata gggctttatt ttatcttccc acaagctgta
    27181 tgttgctcca acaaagaaat tattattttt tcccttttta ttggttattg ccaaaaggtc
    27241 tttattgtta tttagtgtca tagcaaactg cagggtaaga ggaatcattt ggaaacgatt
    27301 taacatcgtg atattgaaat tgttattttt attatccgtt aagtccgaat cgcttttttt
    27361 actgttatcg gttcccattt ggtaaccgat atccacttga gtaggcacat aaggaatttg
    27421 agtaatatta taaccgatgc cgaagattaa gttttgggac ttgtttttaa ctttttggaa
    27481 cgactctttt tcgtatgcga tatccttgtt ttcctcattt ttgccggaat tattgtaata
    27541 ggcaaatttg aaatatggag cttgaggtat gcgaatgaga gattggagat accaggaagt
    27601 tgtattattg gtttcacttt tatgcttcat caggttatct tcaatcaaat tcaaacctcc
    27661 gcttaagata aaatatttag aaacattgaa gttatccgta atggagatga ctttgctatc
    27721 gggaatttga taggcagaac caaaagcatt gaaagcagaa ccggtttcgg aatattggaa
    27781 attcaagaga ttattataaa agcaagttct aacataagta agccaggcaa tactggcacg
    27841 acctggcaca ataggaatca tatttttatt gatgataaac aatctgctaa aatcctgagg
    27901 atcgaggaag aaatctttgc cgagatattc ttccagttct tcctgtgtca ttgctcccgg
    27961 aatagtgttt gtattccaaa gcgagctagc aatttcagct cctataacca aatttggttt
    28021 acggatatgc atctgggtat ctattcccaa aactgcattt tcttgtgcat tagtagtgta
    28081 aattttactt tcattaccta cggtatctac tttggtatat tgatagtaag tgctatccag
    28141 agaactgatt acatcattat gacgagtaaa gttaatgcca aagataaagt tatcctcatc
    28201 acagtattga atacgagttc caaatgcctc ctgtttgaat gttcccgatt tctgcatccc
    28261 gtctacttca gaatcaagat caagttcgtt tgtcgttttg cgaactgtct gtccgtgagt
    28321 tacagataac gataaggatt tgtaatataa gcgggaataa attcccctta agttttttcc
    28381 attcaaagtt aattgactga aggtcggagc ataatcacca gcaaaaactt ccagatgagg
    28441 aatattgaaa cccagggtat aacgattcac aggttgactg ttactatgct ctaatgatga
    28501 gaaatagagg tttgcctgca tatttaatat tccgtagttt ccatagaaat cagcccaggt
    28561 agcggcatcg ctttttgtgt aaccgagagc atcatcattg gagtaattat aataattgga
    28621 ggcaaaattc acagttccct gataagtaaa ttgctttagt gctttacttg agggtaaaat
    28681 ttcggttgac cagataggag aatggactgt ttttccctct tttcttgctc taataaccgc
    28741 tttttgggtt ccgctttgag gtgtttcatc tttataaaca atcaggggag ggctaatttg
    28801 agacaaagaa gtaacatttt tgcctccaac ccaaacctca atgctggcag gatcaatttc
    28861 ttcactaatg gcaaagaaag aaacgaccag catatagcca tcatctgcag aaataatggg
    28921 atcatcggtt aaaagaacaa aaccgggttc tgcttctccg aaggcaatat ccggttccag
    28981 aatgtagttg ggcactgttt cattttgtgg aggaaagtat tctttatttc cctggctcaa
    29041 ttcaacgcaa aagtaatatt ctatcacatc agtagttaaa taatcagcag gcagagtagc
    29101 aataaaatag ggcgaaccag gactatcctg tttcatttct tcggtcatcc agcgattgga
    29161 tttaccgata cgataataga ccttcatttt ggctatatcg cttaaaccct gttgcacttc
    29221 caccttcaaa tcgcattttt gatggaaaga ataaacctga ggcgaaatat tcatcaaggc
    29281 aacggcaaaa atttgagtag cgaggataat tgataatatc agcaataaat attttttcat
    29341 ctttatatcc tcctaccagg taatatcaat atacctggtt tggccattag gagcaacaac
    29401 cggtatacgc attttcctga tttctttttc ctgcattgat tccagttctg tcaattcaat
    29461 ttcctggatg tcttcttccg tactgggtct gatttcaaaa ctgccatctg cctgaatgat
    29521 agcagtattt cctgcagcaa ctgattgggt ttcggtttta tccaaaattt tcaattccaa
    29581 ttccccttcg gtaacaatga atttggtctg ttcctgctca tcaaatttag ccataaaacc
    29641 ggttccttta acggaagcaa cagtagttgg cgtttgcacg gaaaagggtc cggaatcact
    29701 ttttatttgg gaaaaaacgc tcccctggtt tagtttaact gttttggcaa gctttttgtc
    29761 tttggtagaa gcataaacct gcacgataga atgggaaaag acctttacag tagcagaacc
    29821 gtcaatatat ttataagcag caaaggattc tcctccggtg cgtatttcat cattattctg
    29881 caaaaaatca ccggttttat gtttaatcac ttttgctgct ctgcgtaaat ccactttacc
    29941 tttagaggcg gaaataatag ctactgattc cacagctaat aaattaacgg ttaaaatgag
    30001 gatgatagcg aaaagaaata tctttttcat ttttcctcct cttcagtcct tcaattccac
    30061 tgaaatttcc tgtccctgca aagcatcaat cagatttata gcttcctgtc ctgaaaattc
    30121 tttaccatct ataatcacaa ttcctgcagg aacataacct tgcgtgtaaa gatttaacaa
    30181 gatactattt tgtagcatat tgaggtgtgc ctgaaattct gaaatactgg cggcggcttt
    30241 ttcttcatcc acaaactgga aaacatacca gttactggaa aggatattat taccggtgtt
    30301 ttgctttttt gggttatatt ctgttgccag agcagttgtt actttccagg cataatagtt
    30361 gccatctgta aaaggcagga aatcagcaaa accgctgttt tcttttgtat tctctggggt
    30421 ttcataaaaa acggctcctg ttctgtctat agtatttacg gtaggcggat tgttaggagg
    30481 aaactctttg ataatgagct tataatcgtt aaaatcagtc agctgtgaat tccataaaaa
    30541 gcttatggga attccgttaa ctaaagctgg tgttttacca ataggaactc caggagaaag
    30601 caaagtaata tttccagcat tacgaattat gattttgaat tcatcataag gtaaattagg
    30661 atcattagtc caaggagcag tggaaaattc tcttacccaa accttgaatt taaggactcc
    30721 atcgggaaaa taacctgcca aaacagctga ttttaaggtg gaactgtttt ctattgcatc
    30781 ttttactgtt atttttgcat atccttcttt ataatcaaaa taggtgcttg cctcgttggt
    30841 tattaaatcc ctgttagtta atttccagga attctgagta tttatggagc tgaaaatagt
    30901 ttctaccaaa ttaatattat tccactccag aactaatttc aggtctattt ttttaggaac
    30961 cgtgcctgta tcaatagtta gatgggttag aataggttgc tggtcagggt caaaaggatc
    31021 aaaactggca gagttgaaac tgtttacatt aatggtttgg ggtgaaaagg taactgagaa
    31081 accttctctt gccacagcta atgttggcaa taacaaagcg ataactaaaa taaaaagaaa
    31141 gcgtttcatt ttttacctcc cttccttatc agcaatatag attatttttt gcagtaattc
    31201 ctgaaaagac attcccgctg cttttgcagc cataggagtt aaagaaagcg aagtcattcc
    31261 aggcagtgta ttgacttcca aaaaataggg tttgaggcca tcgtagcgaa aatcaattct
    31321 ggcataacca cttaaaccga atgctttcca taaccttaag gcatagagct gtaaaagttt
    31381 ggctacggta tcatcaattt gagcgggggc ttcgtattta gtttttccgt gagtatattt
    31441 attggtataa tcataccaac cttccagggg tttgatttcc acaacaggat atgcctccga
    31501 atcaattatt gtgacggtta attccctacc ggggatatat tcttccaaaa ggacgctatg
    31561 tgaatattgc aatgcctttt ggacagcagg tttcaagtcc tccaaacatt ccactctgct
    31621 tatgcctacg gaagaaccag catcatttgg ttttacaatt ataggaagtc ccaactgatt
    31681 ggaaaatccg gaataatctt ccacatcctg ataatcctcc agaaggtctt cccgcattaa
    31741 aatatattgg ggaaccggaa ttccttccgc caaagcaatc agcttggaaa tgtatttatc
    31801 catactgaaa cagctggctt tgaaaccgga acctgtaaac ttaattcctg ccagggaaag
    31861 agctgcctgc agttcaccat tttctccgct acctccatgc aaaccattaa agaccagtaa
    31921 tggctgttca ttttgaatgg cagtaagtaa atcagaaagt ttcgggtaat cggcagggtc
    31981 tatttctatt actgaatagt ccatatttct taaggcagaa gcaatttctg caccgctaac
    32041 caaagagacc tctctttcag gagaagttcc ccctttcaaa acgacaattt tttccatttt
    32101 tacttatcct ttattgcctg cagagttttt attccgcaga caggtaagtt ttcctttttt
    32161 attataccat agcagaagac ctaatttgaa agaaaagctt cagctgaaga ccaagtgcac
    32221 tattgaatct ggcaatttca ggtatgctgc tgccacagaa atccagttcc tgaaaacctt
    32281 ctttttggag aagttctatc agtttaaccg tatgcaaact acaggcacct ttttttaagg
    32341 cattatcttc agttgcctga tagatattat ataccttttt gccatcctgt aagataagat
    32401 tactgctaac tattttacct tgttgggtta aattcatttg ccgtaacatt ccttcccgat
    32461 ataaaatacg gatgaaatca accaatccct ggtaatcgca atcgggattc cagttttttt
    32521 taaccttcag aatttttagt aagccgataa attcctcagt attcaatccc tcctcaaaag
    32581 tataattttg tttttccgct tgggcaaggt ctctgcgttt atcgggaaga ggttttagtt
    32641 cgctgttaag attatggaca aaagtataaa aaggaacagc ttgcatctta ttccacaaaa
    32701 aacctctaat atcgtaggtc tcaggagtta agtttattct aattctctta aagttactgt
    32761 ttaagcccct tgctattgcc atcgttattt gtagggcatc aagcaacttt cttgccggca
    32821 gagaattttc ctccagccaa aggtttaatc cctgataata ggataaagag gagcgtttta
    32881 acactctgca accaagtatt tttttctcgt aaagcggtag aacagcaaca attgtagtgc
    32941 ctttataaca gaccaaatgc catggtatga agttggatat ttcagctacc gtctgcataa
    33001 aaacaggatt gctccaaata tttaaagagc gattgggaac gggtttcgga tatgttaatt
    33061 ctatttccag catcagatgt aggaaccgat tgtatttaat aattctttta gttgaaaggg
    33121 ctttacaata taatcggaag caccatctac aacggattga aaagcagaat caacagtagg
    33181 gtaaccagtc ataatcagac aaaagatttt cgggtcttgt ttcaataaaa ttttcattaa
    33241 ctccaatcca ttcattccag gcatcatata atcaattaat gccagttggt atttaccagg
    33301 ttcaaacttt tccaaagcgc tgtctgcatt gtcagccaaa tcaacttcat aaccgtggga
    33361 cgataaaaaa tcacctatta cttctcgtag taattgatca tcgtccacta atagaagttt
    33421 tggcttcata taaactcctc tgaatttgtt ggttacctta tataaactat gataatcaat
    33481 tctcttaaat agtcaataga aaaatgttat gagagtcgtg aggtcttgcc ttttttttaa
    33541 ttgacataat cagctatatt tttttctatg acattatgat aaaaatatgt gaggttcaat
    33601 atgaaaggaa aagtaaaatg gtttaacaag aataaaggtt atggctttat tattactgat
    33661 gacaacaagg aatactttgt ccactggaaa tctatagtta ccaattctcc gcgggaactg
    33721 aaagttttgg agcaggatga actagttact tttgacttga tggaaacgga caaaggcatt
    33781 caggcaatca atattatcag agttaatccc tgattagatg cctacctttt ttcaggggta
    33841 atggatttta taactttcca ttaccccttt tttagcgatg ccttttcttc cagttaacca
    33901 atcagacctg aaagagaggg attgggatta tttagacatt attcttatca gtggagatgc
    33961 ctatatagac cacccttctt ttgctgcagc aattatcgga cgcactttag aggcagccgg
    34021 ttttagagtt ggaattattc ctcaaccgga ttggagaaat gattcctctt ttaccgtttt
    34081 aggaaaaccg cgtctctttt ttggcattac tgcaggcaat atggattcta tagtaaatca
    34141 ttacacagcc cagagaaaac tgcgtaatga cgatgcttac agtcctgatg gaatttctgg
    34201 aaagcgtcct gacagagcta tcattatcta taccaatatc ataaagcgtc tttttaaggg
    34261 tgtgccaatt gttattggag gtatagaatc atccttgcgt cggattgctc attatgattt
    34321 ttggcaggat aaagttcgca atagcatttt ggcagatagc aaagcggata ttttaattta
    34381 cggaatgggg gagagacctg taacggaact ggcgatttta ttgcaaaaag gagttcctgt
    34441 aagcagtatt caaaatttgc cttccactgt tgttttttct tcttctcctc cggcaaagga
    34501 ggatattatt ttgccagaag cagagaagtg ttccgctaaa gagacctttt atcaaatgac
    34561 cagacaattt gaggataatt atagaacaaa aactatcttt caaaagaatg caggacgctg
    34621 gctgaaacat aatccaccgg caaaaccatt aactacaaag gaaatggata gcatttatgc
    34681 cttgcctttt gcatatcaac cccatcctgt ttatgcaggg cacaaaattc ctgcttatga
    34741 acaaattaag gattctatta cttctcatcg cggatgttat ggtggttgca atttttgtgc
    34801 tattgcctgt caccagggac gcaatattca atccagatcg gagacctctc tgctgaaaga
    34861 agcacaaaaa cataccggca caattaccga tgtgggaggt ccaactgcaa atatgtatgc
    34921 ttcattttgc cgtttggatt ttcctgagac ctgtaaaaga agttcctgtc tggttccaga
    34981 tatttgtccc aatttgcaaa taaatcattc ggttcagttg aaaatcctcg ccaaaatagg
    35041 gaagttaccc cgtatcaaac atttatttat tgcctccgga attcgttatg atatggcagt
    35101 aaatgatgat aactatattg aagccattgc tactaaatat acgggagggc gtttaaaact
    35161 tgcgccggag cattctgttt ccagtgtttt acgcttgatg aataaaccgc agattgaggt
    35221 ctttgaaaag ttttgtaaaa agttttattt ttacactaat aaagcgggac taaaaagcca
    35281 aattattcct tacctgataa ttggtcatcc aggaactact atggaagatg cttttgaact
    35341 tcgtaattgg ctcagaaaaa ataaattaaa ggtagaacag gtgcaggaat ttacccctac
    35401 gcctatgaca atttccactt gtatgtatta taccggttta gattatgaaa caggaaagcc
    35461 gattcatatt cctaaaccct ctgaaatacg caggcaaaag gaactgatta taaaccggta
    35521 gaacgacatt cgcgtcgttc attttgtagt tgttaggcgg taaggaataa atatattgta
    35581 aattagaggt aaacaaaaaa tggtgttcat aatcctaatt attcttatag gatacggctt
    35641 aataaagtta tataagttat ctaaccctta taatcctttt cacccaacag gaacaataat
    35701 agatgaatat ctccaaaaag gtaatcggaa catcaatata gagataagaa taagagatag
    35761 ggacgaaaaa gattaaaagt ttaacatttt tatagtttcc aaaagcaata caatgagagc
    35821 gttgagagca taaacctttc cttaaaaccc aataatgcaa tagaaataat agttaatatc
    35881 atttctccat tcgttaagca catattgtag taaaaattcc gtagaaagca aattagatta
    35941 gttccctatt gaaaattcag ttatatagtt taaaaatatc tggattcctg atttcgcggg
    36001 aaagacatta gaatgataga tattatggaa agatttgaac ccggtgaaaa gcgtaaagga
    36061 cataacttat ctctcttatt aagaatgagt ttcgcaggaa cgaaagatat taacgacggg
    36121 tttgaacccg gcgaaaagtg aacaacttat ctcttttgtt aagaaaaagt tccgtaggaa
    36181 cgacagatac taacaacggg ttttaacccg gtggtaaaaa tccctttaga aatcttcagt
    36241 ttctttggca aattggaagt tgccgaccca tttttattca accgcattat tggggataaa
    36301 aattaaaggt tatagcagaa tgcctataat cgccactctt tattgaagtt atatattctt
    36361 ccttaggagc attgcttacc cattgcttac ccattgctaa tcccatccgt aatgggtgag
    36421 caatgagtag gtagtgctat tatagaagaa aagcaacttt cacaatgaca gcatattaac
    36481 ctgaaaggga gaaaaaatgg gtggaaaaaa agggaatttt aatgccatct aaaaacaaaa
    36541 aaaacgggtt ttataacata ttgcaagata aaggattata aatagaaaat agataaagaa
    36601 agtgcttgac aataacacta tatcatttac tgtggcaaca aattaaggat tcaaattcca
    36661 ttgagattca tctatttgga aaaaaatcct tagctttatt cataaaatta taggagacaa
    36721 tagaatatgc aaagctcaac ttatacagcc agcaatatta aagtgctgaa gggtctggaa
    36781 gctgtccgta aaaggcctgc aatgtatatt ggaggcacca gtgaacgcgg tttgcatcac
    36841 ttggtttacg aagttataga taattctata gatgaagcat tagcaggtta ttgcgatgaa
    36901 attatttgca ctataaccac cgaaggaaat attagagtgg aagacaacgg acgcggaatt
    36961 cctgttgata ttcaaaaaga tatgggagtt ccagcagtgc aggttgtctt aacggttctg
    37021 catgcaggag ggaaatttga cgataaaagt tataaagtag ccggaggtct gcatggagtt
    37081 ggtgtttcag tagtaaatgc tctttcggaa tttctggaag tgaatatttt taaggacggg
    37141 aaagaatact ttatgcgctt tgaaagaggt cttccggtaa ccgaattaca ggttttaggg
    37201 gataccaata aaaccggaac agtagtaact ttccgtcccg atccgcttat ttttgaaaca
    37261 acggaattca gttttgatta tttaacttcc cgcttaagag aac
//
LOCUS       DAOPFL010000007        34522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1172_length_34522_cov_94.717150, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000007 DAOPFL010000000
VERSION     DAOPFL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1172_length_34522_cov_94.717150"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(754..1968)
                     /locus_tag="PLG69_01375"
     CDS             complement(754..1968)
                     /locus_tag="PLG69_01375"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HPI42190.1"
                     /translation="MKNILCNIVNPISPEEIQFLPKQVISIEESKISAITSLPEFQGR
                     IDEDRSNEYALPGFIDLHTHLSQYYIRGLYEPALLPWLNKYVFPEEERSKNIEYAEKL
                     SRDFFSAMLKAGTTTCVIYTAPFFSACDMAFEIAKETGIRALIGMTMMDMNCPENLPQ
                     NSHKTLEESILLYEKWHGKNAKLDYIFTPRFAPTCSLELMKEVAKYAIEHNAWIQTHL
                     SENKEEIEMVKEIFGFKNYTEVYQKAGLLTQHSIFAHCIHLGDEEIKMLAENKCKIAH
                     CPDSNFFLKSGEFPLQKIEEAGIEYGLGSDVGAGTSLNMLYHTKMMNYRQSDYPVLPA
                     KALYHITLGSAKLLGVDKIIGSLEIGKEADIVFLKPPLNYPLKNNGISQLVFCGQEFS
                     LTETLVAGRNKE"
     gene            complement(1965..4634)
                     /locus_tag="PLG69_01380"
     CDS             complement(1965..4634)
                     /locus_tag="PLG69_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HPI42191.1"
                     /translation="MRYINLFIFILLVFGCYAITNEEINFLCDKEQFQILAKYDETIQ
                     AKVLYGDEEDLQTVLNYSHRANLPELAKQCHKRLALNYNSLEDALQWLMLCETEEVDS
                     LTFYNDFSAITEALTDSLDSAVWLYYTKRCSEEELYAKIATTKKYNTIIEAMAKDLID
                     EISIERNDSLAFNLLNEFETKYPHSRYRSIALYYKLYHFANRKNWQELIKALPQHTNL
                     DPVSAYIASLFLLSPTFRKDFEGEENLLELAEQYLTLALSDCEQTLLYDIYSADDWKA
                     RVLQQQAKLLFYRIIEPYGLFGDELDIPMLEKNKLKQQQELLALLAQVQFSNNNRGEQ
                     AEKHFWTAKALLLTGKKTDKQKAAEHLTQCLISGSPRNRYDVEAMALITKLHTDLKIK
                     EEPLKWMRKMMNYKGICFEDKSENAGLNGKGYTRVALADYNSDGFTDILFNGKYLYRN
                     EGKMKFTELTDSAGLANLSSNGGIFADFNKDGLLDLVSYSHSAEGRGDQLLKNMDNIR
                     FVNVNERAGDIDDTYPTEAVAWIDIDQKGYPSLYSANYEIWLKRSGFPDYFWKNDGGY
                     FTDKSDAYGFREPPYTDNPGLPGRGVAPADFNNDGKQEIYVTNYRLSRNFCWQQIDTL
                     FVDVAALYGIAGTYKNGYYGHSIGADWGDYDNDGDLDLFVANLAHPRYIDISDISMLL
                     RNDGLTYRIVEKDTIYYWQFTDVTEQAGIKYDELHSDPLFFDADNDGFLDLFITSVYE
                     NERSYLYHNKGDGTFEDVTWLAGARVYNGWGNACADLDRDGLQDLIVGSGNGSKILHN
                     GTITKNKGLFIKPYWKNDIVLLENNAENFTKVPNSPAFGTRVEITVKRLFHKKQKFIR
                     ELCSAKGTGSQNAPELHFGIGKAKVIKIRKVEQ"
     gene            complement(4631..5512)
                     /gene="hslO"
                     /locus_tag="PLG69_01385"
     CDS             complement(4631..5512)
                     /gene="hslO"
                     /locus_tag="PLG69_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPI42192.1"
                     /translation="MTKDYILRGTVHNNNFRVFAVNSTNCVQTARDLHDLSPIATLLL
                     GRMISAAAMLSWDLKDEESEITMRIDCEGDIQGAIVICTQNGFLRGYTKNPRLFYATR
                     EDNFKVGKAVGKGTLTISKDKKGTRSYFSTIELINGEIAEDLAYYYQQSEQIPTAVNL
                     GILIDKEAKVRSAGGFIIQQLPYADKKIADVINENIAQTPNLSDLMDMGLSIPEILSL
                     FVFKGLQWQINDEKEIAYRCNCSYELFSKALLLLGKEELQSLKEGIKPVCLYCNKEYN
                     FSSEDIQSLIEQLQEQK"
     gene            complement(5505..6998)
                     /locus_tag="PLG69_01390"
     CDS             complement(5505..6998)
                     /locus_tag="PLG69_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPI42193.1"
                     /translation="MKRNYLIILLLLCLKLLAGAQFIKGELYLEINLTASPQLTITPK
                     GKNANITIAEMDGLISRNFDSAITINIMNPETLVHYAILDFTEIVYAAQDDEQAVVRD
                     FFAWENSRLKIKKELQHFLPESFTTEEEAKQYASIQGIPIDKIKEVQLLNSTVQIQDA
                     TGNLCYMETPLKIHSEGEIYFNRDSYDYSGDFILKTVKKQIILNQLLPLETYLAGVVQ
                     NEIGDNAPAEALKAQIVAARTHALTLLLNNKHKTDGYDLCNSTHCQVYKGKYLLNEQI
                     LQAVYECASEVLFYNGNLAETTYHSCCGGKTEAADIIWKGKPIDYLKGVTCLNAVNSF
                     DLSKEKDARAWIDKPLAEEEMSSWERGALSWHKSISLNQLSKNAGLSSISRIEILQRG
                     SSGRITKVKLIGNETISLNSEYQIRQVFGNLLSSFFYIEGSYQIANGTVIIIPKPNIK
                     IKGRGAGHGVGMCQVGALRMARKGTKYEEILQHYYPGTTINKDWIDD"
     gene            complement(7101..9137)
                     /gene="metG"
                     /locus_tag="PLG69_01395"
     CDS             complement(7101..9137)
                     /gene="metG"
                     /locus_tag="PLG69_01395"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPI42194.1"
                     /translation="MKYLVTSALPYANGKLHIGHLAGAYLPADIFVRYLRLKGEDVIY
                     ICGTDEHGTPISITADKEGITPQEVVNYYHNSIKKAFEGIGIEFDNFSGTSRPPHYKL
                     AADFFLQLYNKGYIKPKNTLQFYCEKDKRYLPDRYVEGICPLCGFEDARGDQCDKCGQ
                     IYETTTLIEPKCKICGSTPVIKETKHWFLLLEGFREELKRWLATKDYWKENVRNFILN
                     LLDQGLVERSVTRDLSWGVPVPLPEAKGKVLYVWFDAPIGYISATVEWAEKIGQPEKW
                     KEYWLNPETRLIHFIGKDNIIFHSLIWPSMLMGQDTTYCLPYDIPANEFMNLEGKKIS
                     TSKNWAIWVDEFIQDFDSEYLRYYLAVNAPERQDSDFSFKDFQNKINIELNNTLGNLA
                     NRVFAFANKNFEGKITACALSETANMAIAEADKLLKEIEENYLDYQVKKNTRLIMDIA
                     RLGNRYFDENKPWAQLKENRDATNETLYVCSNLLAKISVAFSPIMPRSMRYLRQMMNL
                     DDNCKFSEAYNLLGDIVLNEIKPLFRKIEDKEIEAQIAKLHSQAIINEGDKSDFNTPE
                     IAPLKELISYDDFAKLDLRLAKVLTAERVPQTDKLLKLKVDIGIEQRELIAGIAVDYE
                     PNNLIGKTVLMLVNLEPRKIRGITSQGMILAADSNGKLLLLIPDGEGIPGAVVQ"
     gene            complement(9134..10303)
                     /locus_tag="PLG69_01400"
     CDS             complement(9134..10303)
                     /locus_tag="PLG69_01400"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPI42195.1"
                     /translation="MKKFGGTSVGNIDLIRNIAHRLTSEYHKGEGLVLVVSAMAKTTD
                     ELFSLAYGISKHPSRRELDMLLTAGERISMSLLSLALMEEGIPSISFTGSQSGIITDE
                     KHGNARILKVNAFRIQEELEKGKVVIVAGFQGVSTSKEITTLGRGGSDTSAVALACYL
                     KANKCEIYTDVDGVFTADPKLVEKPKLLRQISPELMLTLCYNGSKVLHPRAAEYALKY
                     GVEVEVKSSFTFAPGSLITNKKTENNEENNMEERKVTAIAHKNTLLRYTFPLNDKVLK
                     TLSDWYYEIYKSFVNEGLLELFVESKYETEVDYFLQERDIKPISKKGDFAFVNLIGLG
                     LGHDPAFLATVMENCQNFNILRSFNNEQSIELLLPDQNCAAAVKTLHKIYVEEQV"
     gene            complement(10704..16166)
                     /locus_tag="PLG69_01405"
     CDS             complement(10704..16166)
                     /locus_tag="PLG69_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42196.1"
                     /translation="MKTTKIAILILMCSFSALVAGTIIPIEKPLSVVSQNAGKIKLQL
                     QVPELQIEDIDNSNFKILSMQGAETTAETGFPELPVFSAWIAIPPKGDIEIKVTGGEI
                     ITQKGFIPKPVFATKEQEIASEYNKTAYHSASLYPANSYSYSQPQIIRDFRVVQITLN
                     PVQYVAETQELKIQKQMEVEIEVKDRQGINEMDEYNGYSYAFTNLYESMIANFDYYRN
                     TMMAPQPARILLIYGNNTDAIFIAKLNEFVAWKRQKGYDVNFVSTVETGGASTQAIKN
                     YIQTQYDNPSTRPDFIILLGDTNGSYAIPTYTEQMSTYGGKGDYPYTFLAGNDYLGDA
                     FIGRISAENISQLDVLFTKIYAVEKNINVSGNAGAWVNKMLLIGDPGNSGISTIYVNK
                     FIKELAKRTNPDYTFLENYSSGFPAYIDNGFNQGVAFFNYRGWLGMSGWSPSASLING
                     NKLPHSTILTCSTGNFENNTATSESLIRLGTSAAPAGAITAIGMATSGTHTTFNNCLN
                     AGIYDGIFTYGMRSMGEALLNAKLYIKQVYGSTFDQQANYFAHWCNLMGDPTVEVFTQ
                     IPDIFNLACADILPLGSNLLDVEVTNSFGTGVKDACVTLYSTAQNIIVGRDYTDSEGK
                     ATINISGGMLNELTVTVSKHNYKPFQKVVTIDPTGSLVYLNKIVIDNGTNGSYGNGDS
                     FATAGETIALSLEIKNTTSATVPATTAILSADDPYITILNSESSFDAIDSNATLWSNS
                     VFTFSIVNNLPAQHNVRFTLQLTTTDQNVYSFVFHTVIYNALLLVHNSSISAGGNNIL
                     DPGENGFLSLTIKNNSIAPVFDVYAELSSLNDLITVTDSTSYVGSILGNSLGTTVEGF
                     EVFARPLLIPGMQMPLRLHLYNEWGFDQYTEFNIQIGSVNSHTPLGPDAFGYFIYDIT
                     DTAYPDCPVYEWIEINPSLGGAGTKITAFSDPGSSTDEGDQTGAVTLQQIDLPFTFPF
                     YGIDYNQITVCVNGFIVMGFTENGEFRNYHLPGGYGPSPMLAPFWDDLCLINDAGIYK
                     YYDSQNHRFIIEYFKMRNGYNRSSLETFEVIFYDPVFYPTSMGQGMVKFQYQDFNNVD
                     IGQGGYSPSHGNFCTIGIKDHTNTIGLEYTFNNQYPSAAAPITNFSALLITTAPVLYE
                     SPFLVLNEVIINDTNFNQIAEPGETIELGIKLMNIGLGTATQVQLSAFCASEYAVLIN
                     AISNYSNILGDATAINNTPITLQIDANCPNAAEIPINLHISISDNSWDFTVYITVHKP
                     DIQYSGMYMNDSITGNGNGLIDPGETLDLIINFANNTPVSANNISSTITCLSEYVTVH
                     NNSCIISSLPAGGISQAIYNITISPNVIVGNNLTFYLTYLADFTSVHSEQLVLSVGTT
                     GMNADFENNNGNFVPNPSSNGWEWGESTVAGAHSGTKVWGTRLNSTYPNNANYQLTTP
                     SVYIGSNFMLEFWHYYNSEANYDGGNVKISTNGGTTWTLLTPVGGYSNTNLSVLNGPG
                     YSGNSGGWVFARFNLSIYANQSVQFRFTFASDSGITGDGWFIDDVRTTGFVEFAGKVF
                     GTVTSSNSAIDFSIVNVQNSASWISHPDNEGNYAIYLPIGSHSIWASADGYKNSTSAV
                     ITISLANPLVQQDFYLGYFTPVSGISYMLADSVLTLNWNPPSEPEFPLIHYEIYRRIN
                     AGAFALAGITVIPVYTETLRELGTDYYFYVVCVYSEGSSMPSEILHYNYLTDIEDNVN
                     PALITQLLNNYPNPFNPETTIRFTLQETAPAKLYVYNIKGQLVKKLIDEVLPSGMHQI
                     VWNGKDNNNRNVASGMYFYRLESKNYSCIKKMLLLK"
     gene            complement(16263..17285)
                     /locus_tag="PLG69_01410"
     CDS             complement(16263..17285)
                     /locus_tag="PLG69_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42197.1"
                     /translation="MPKSYIITLAIIVLILLSSCAKRNTEYQEGDWLTLRNVLPIVGN
                     PLDIKIDDNYIYVAHDQGGMSRISRTDYHIQWITEFWAEDGSQKVLGRIKKVAVVPEH
                     NFMFLVDTEATDAIRVINTSDPDSLIYILDFIGGTSNIKDLDSFAITPVPFLQGTYKM
                     VCAYCAAGSFKYDYYDGTIFNSNVFTISPGAAAGFKLTNNYIFIAAEQLGLYIYNRND
                     RQLIGNIHLSGEAQKVEVNNNIAYVAGRQSGLHIVDVSNPASPVLLSTFDTSGYATSL
                     AVSGNKLALSSGGGGIYLFDVSIPSKPQLLQHLTSCGYTNTVKFMDNKLVVGARDQGI
                     LIYEIK"
     gene            complement(17649..19298)
                     /gene="hcp"
                     /locus_tag="PLG69_01415"
                     /gene_synonym="priS"
     CDS             complement(17649..19298)
                     /gene="hcp"
                     /locus_tag="PLG69_01415"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPI42198.1"
                     /translation="MSMFCYQCQETSRNLGCTMRGVCGKSETLANLFDLLIYGLKGLA
                     YVSVKLQEKDRYYPEDSVFVMSALFKTITNVNWDEENIKDTIETTLAVRDKRKDELCK
                     LMEGKCDTCPEAVTFQLKRDEYDEFAKKVGVLRTENEDIRSLRETIIYGIKGICAYGE
                     HAAILGFQDDDVNKFIMEGLSATLDDNLTVDQLVNLVLRTGENAVKVMAKLDEANTKT
                     YGNPEPTKVKLGVGKNPGILISGHDLKDLEELLKQTEGKGVDIYTHGEMLPAHYYPAF
                     KKYKHFIGNYGDSWWHQLEDFENFNGAILMTTNCLVPLRKENTYLERLFTTGTVNYPG
                     AVHIPDREKGREKDFSPVIARALKCQPPKELETGEITGGFAHSTVLSLADKIIEAVKS
                     GAIKRFVVMAGCDGRMPSRKYFTEVAEKLPKDTVILTAGCAKYRYIKLPLGDINGIPR
                     VLDAGQCNDSYSLAVIALKLKEAFGLDDVNKLPLSFDLAWYEQKAVAVLLALLSLGFK
                     NIILGPTLPGFLSPNVAKVISDTFGLQQITDADSDIAKMLA"
     gene            complement(19315..19632)
                     /locus_tag="PLG69_01420"
     CDS             complement(19315..19632)
                     /locus_tag="PLG69_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPI42199.1"
                     /translation="MKSRTWQDVEPKVNANGILGSKIYEAPEGEIVHLNLAPGAHLKK
                     HITPVNVAFYVLEGTATLEIGEEKQSFPEDTLIESPKDIPHGVFNEGNSNLRLLVIKM
                     PKP"
     gene            complement(19642..20229)
                     /locus_tag="PLG69_01425"
     CDS             complement(19642..20229)
                     /locus_tag="PLG69_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPI42200.1"
                     /translation="MELTERQKDIIMAGIAIIANQGYEKLTTKNLATKIGVTEAALYR
                     HFKSKRDLVTTILGYFEELSNKVLQEIREKDYSPLESIRHFVEDRYILFSEHPNLAKV
                     MFSEELFKNDPTFKGQFQCIMHKHKQVMENYIIQAQQEGKIRSDLSPTQLFRIIVGSM
                     RFTVTQWNLSDGAFDLKKEGSELIETIIKLIETKQ"
     gene            20565..21635
                     /gene="hydE"
                     /locus_tag="PLG69_01430"
     CDS             20565..21635
                     /gene="hydE"
                     /locus_tag="PLG69_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPI42201.1"
                     /translation="MRPDKNKLIELLSTEGEENLQTLYNQAYKVKKKYVGTTVYLRGL
                     IELSNICSKNCYYCGIRSGNKELKRYQISYEEAIAEAKLCQQMHYGSIVIQAGERNDK
                     PWIDFITKLIIGIKELSEGKLGITLSLGEQKREVYQQWFEAGAHRYLLRIETSNEELY
                     KSLHPAAQSFETRLKCLHSIKECGYQVGTGVMIGLPGQTIEDLANDILFFYDEDIDMV
                     GMGPYIPHHNTPLKHLVDSFNPAEALKMGLKMIAACRIALKDVNIASTTALQALHPEG
                     RELGLKAGANVLMPNITDTKYRKGYQLYEGKPGLNETAIQYQKELEQRISALGETIGY
                     DEWGDSKHFYSREVQRSMKNEK"
     gene            21770..22093
                     /locus_tag="PLG69_01435"
     CDS             21770..22093
                     /locus_tag="PLG69_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPI42202.1"
                     /translation="MLPGGKNMQQLMKQAQKMQAEIMKQQEELANTVFSASSGGGMVT
                     VEMNGQFEVVSLKIDPQVVDPEDVEMLEDLITAAVQEVLNKVNEASSNVMSKVTGGIN
                     IPGLG"
     gene            22094..22693
                     /gene="recR"
                     /locus_tag="PLG69_01440"
     CDS             22094..22693
                     /gene="recR"
                     /locus_tag="PLG69_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPI42203.1"
                     /translation="MLFSENLEKLIQSFNRFPGIGRKTAQRLAWYLVAQDKNFALNLA
                     ETIKTTVESFKTCSQCNMLSESDPCPFCISPDRSDSYLCVVENSSDIQILEGMNEFKG
                     RYFVLGHLLSPLDGYGPEQINTAMLLERIENLHPGEVILALKPSAEGEATIHYLWELL
                     KDKGISITRLSTGIPFGGDLEYSNLNTLANAWKRRYTVQ"
     gene            22662..23285
                     /locus_tag="PLG69_01445"
     CDS             22662..23285
                     /locus_tag="PLG69_01445"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPI42204.1"
                     /translation="MPGNADIPFSNMFTGIIEATGKIVTHQLISGKKLITISRPAIFD
                     DLKIGCSIACDGICLTVVQFDDKAFTVEIMNETLQKSTAKNWTSGKILNLERALKLNS
                     RLDGHFVQGHIDRELRLISHLKREGTDYLHLELPSQERNLIVPQGAIALNGVSLTIAE
                     IRNNYFSVALIGHTLTHTNLGTIKTGEKVNVEYDILGKYVLAQIPKG"
     gene            23329..24612
                     /locus_tag="PLG69_01450"
     CDS             23329..24612
                     /locus_tag="PLG69_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPI42205.1"
                     /translation="MRFNKTFICLFFLLIILFLISGCGNKRSPTGGPQDIDKPVVLES
                     SPAEFGDISSGYIEISFSKPMDRNTLANSIYIYPPVQNKKITLDGANLKIHIKEPLKP
                     DTNYFITLTKRLKDLRGNTLEKNQTLIYSNGKLATNRIAGTITYEEPTDKGLPLELTL
                     LSADSLLVLSSVIQGGAYAIDNLNPQKHILRTYIDKDFNGRYDFGREPFFEGITDGSS
                     VATLNITMAYGDTTRAKISQVNVLSDRELQVILSEDIKSYDALNIQTKNTPLSIAYQL
                     IEGYIINLLTAKMDSTEYTLTLTGVKDKKDNISNKLETKFKVRPVADTLAPKIIFTNP
                     RNGASVNTLLPVLEIHFNEIIPKANIKAKLLCGNQEIPLKHLSETGRIHRFQPPKELE
                     NYKSHLLIISSETTDFSGNHLKNDYELQFLPLLRK"
     gene            complement(25134..25604)
                     /gene="rpsG"
                     /locus_tag="PLG69_01455"
     CDS             complement(25134..25604)
                     /gene="rpsG"
                     /locus_tag="PLG69_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPI42206.1"
                     /translation="MPRKHRAVVREVLPDPKYNDITVTKFMNCLMVKGKKSIAEKIVY
                     GAFDIIAAKTKEDPLVVFKSALDNVRPLVKVVSRRVGGATYQIPIEVNEKNGKALAFR
                     WIIANARARSEKTMTEKLAAELIAAHKKEGASIKKREDTHKMAEANKAFAHLRW"
     gene            complement(25621..26007)
                     /gene="rpsL"
                     /locus_tag="PLG69_01460"
     CDS             complement(25621..26007)
                     /gene="rpsL"
                     /locus_tag="PLG69_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPI42207.1"
                     /translation="MPTINQLVRKGRAEVEKKRKNRALMECPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLVNGFEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DSAGVEKREKSRSKYGTKRPKAKTVK"
     gene            complement(26050..30531)
                     /gene="rpoC"
                     /locus_tag="PLG69_01465"
     CDS             complement(26050..30531)
                     /gene="rpoC"
                     /locus_tag="PLG69_01465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPI42208.1"
                     /translation="MLKESKHEIKPNEYDAVRIKIASPETIRNDWSHGEVTKPDTLNY
                     RTLKPEKDGLFCERIFGPERDYECACGKYKKKRFQGTVCDRCGVLVTTSRVRRTRMGH
                     IDLAVPIAHIWFVKSAPSKIGTLLDMTVKDLERVLYYESFIVIDPGDSPFEKMELLEV
                     DEYYEIKDKVADNFLALMGGEAIRELLKRINLKNEALDLRTRLKFEESALRKQKLINK
                     LKIVDAFIKSGNRPEDMILEALPVLPPTLRPLVPLEGGRFATADFNDLYRRVITRNNR
                     LKALLEIRAPEVILRNEKRMLQEAVDALIDNGRKARPVRGRGNRPLKSLTDQLKGKQG
                     RFRQNLLGKRVDYSGRSVITVGPELKLYQCGLPKEMAVELFKPYLIDKLQKIGEVDKV
                     KNAKKLIAKKQPEIWSILEEVIKDYPVLLNRAPTLHRLGIQAFMPVLTDNKAIQLHPM
                     VCVPFNADFDGDQMGVYIPLSVESQIEARVLMLSTRNLLLPANGRLAMAANQDIVLGC
                     YYLTMEETPPPVEIEKLRHFYGPDEVIAAYEAEEQFCSSKGETVYERNLDLHTWIRLK
                     LESEYIVTTVGRVILNQIMPPEVGFQNIVFDKGKLNDLAMLCFDAVGQWRTAQVLDQI
                     KELGFHYATRAGVTFSFDDIVVPKRKDEIIAQADQEVKKITDLHQKGGITENERFGRV
                     VDQWKKTTVRVTDEMMEELSKTRNGLNSIYMMYKSGARGSKDQIKQLGGMRGLMDKPT
                     KIGSTGGADVIETPIKSNFKEGLTVLEYFVSTHGARKGLADTALKTADAGYLTRRLVD
                     VAQNAIITIEDCGTTRGVNMTALKEGNEIVQTLAERIQGRTAVDDIVSPITGRILVSA
                     GEEISNKMARTIQNHGLISVHVRSILTCEAEKGICAKCYGRNLATQKPATIGDPVGII
                     AAQSIGEPGTQLTLRTFHIGGAASTATELAEVVASHDGIVKFDRMNTVINTENQLISV
                     SYLGKILIVDENDENKVLEEFKVEYAATVHVQDGQKVAKDTKLLSWDQFNNPLISTAK
                     GVLHYENFIKDITYKEEYNDITMARELTIIESKDRRKQPQFRIVGEDGTVRLIPLPTG
                     LIVRVEDGSFVHIGDILGQTSRMTIKQRDITGGLPRVQDLFEARVPKDKAKISDIDGI
                     VTIGGLKKTGRDIFVTPPNGLFAPCDGKVVVLYDEDKNQYIAVLPDKAVYSENDGKVS
                     IIEENRKKIIQVAKRNQNPVQYPVPKGLQILVKEGESVKKGTPLCGKLYAVPPEQESI
                     VETGDNVTMHQPLAGRKYIIPSGKRIIVHQGDYVESGDALSDGPLDPHDMLVKGVIEA
                     QILILNEIQEIYRKQGVKIDDKHVSVIIRQMFKKVRITDSGSTSFLQGDIVDKVLVEK
                     ENREAIEFGKTPAQFEQLLLGITKTSLLTDSWLSAASFQETTKVLTKAAIEGRVDHLE
                     GLKESIIIGHRIPVGTGTKLYNNMLKKAVNEGKTVAQIIDEFAHRSKEEESGDYLDF"
     gene            complement(30545..34324)
                     /gene="rpoB"
                     /locus_tag="PLG69_01470"
     CDS             complement(30545..34324)
                     /gene="rpoB"
                     /locus_tag="PLG69_01470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPI42209.1"
                     /translation="MRKVKSYSRISGRFAELGIPEVEIPNLLAMQVDSFNDFLQKDIH
                     PQRREKKGLQAVFENIFPLEDSKGNFLLEFIEYNVLQEKYSLEECRERNLSYQAPLKA
                     KMRLSVFENREGVREHKDTLEQEVFLGEIPLITEQGTFVINGAERVIISQLQRSPGVF
                     FSEEKHPSGKTLYSAKVIPYSGSWLDFDIDIHDVMFVHIDKRRKLPVTTLFRAIGIST
                     NEDLRKTFYKSEKLNLKEAKGRHLFADIYEKGNPEPIAMANEQITDSLIKFLSERKIT
                     KVEVIDYNYEIARKVLENTMAKDPTTNQEEALKKIYNLIRPGEDAPLEAAKQLVDRMF
                     FNEKRYNLGEVGRYKINSRLGINVDPKIMTLTVEDFVYIFKTLINIYHDEDEVDDIDN
                     LANRRVRTVGELLQEQYSIGMAMVARIIQERMAISNIDEVTVHDLVNSNALISVVQSF
                     FLTGQLSQFMEQTNPLAALRHKRALSALGPGGLARERAGFEVRDVHSSHYGRICPIET
                     PEGPNIGLIVSPAIYSRINHFGFLETPYRKVVDGCITDEYEYLDASQEEKYIIAQSDV
                     HLDDNRRITDEMIFARDRGEFVQVSPNLVHYMDVAPQQMVSVSAALIPFLEHDDANRA
                     LMGSNMQRQAVPLINPQAPLVGTGMEKIASMDTSSIAVAPYDGIVTKVTSAFIDLKPI
                     NEKDEAYYLSTGNRIHLKKFVRTNQDTCINQRPIVRVGDTVKKGQPISDGACVEDNRL
                     ALGTNLMVAFMPWYGYNYEDAVILNEKVASEDTLTSIYIEELEVLVRNVKNGREELAY
                     DIPNVPAQALRNLDKTGIIRVGSVIHAGDIIVGKVTPKSVEIDPSPEENLMRALFGDR
                     AGDFTNSSLKAKPGMEGVVIDVKVFSRLEEGSDLEEENEDKIEKLKEELALRRSKIEE
                     FKEEKLSAVLVGQIAKNIWDEKTNIPFIAPGKKITKQDLKRINFKKLDLDVELVEDRE
                     VNDYIYSQIVLKIKQSLEQSDNIYKKSRERIKHGDELQYGVRKMVKVYVAKKRKIEVG
                     DKMAGRHGNKGVISIIAPIEDMPFMEDGTPVDIVLNPLGVPSRMNIGQIMETHLGWAA
                     KTLGFEVETPIFDGASNEDIVSELRKAGLPEDGKTVLYDGKSGEPFKERVTVGIIYMM
                     KLNHLVADKMHARSTGPYSLITQQPLGGKAQHGGQRLGEMEVWALEAYGAANVLEEML
                     TIKSDDVDGRNNAFKAITRGENPPPPGVPESFNVLISELKSLGFDIEFIKEEENK"
BASE COUNT        10416 a         6952 c         6084 g        11070 t
ORIGIN      
        1 ccaaaagggt ttctggactt atactaccaa aacacgggaa acatcataat aaaaaaactt
       61 ctcacagatt acacggattt cacagataaa gtttagctac cgataatccc gaaaacccgg
      121 aaattcagga gttgttacta ccgaaataaa tattgtccaa aatctataat cataaatcat
      181 aagtgcgata tacaattcca ttttatcaca actgcaatat tagagttaaa tacattttcc
      241 ctctttcaag aaagataagt tttcgctttc ttgactatct aaagacaaag taacattcaa
      301 gtaacaacca agtaacgaaa caactgcagt gttacttcag tgttacggag tcgttactta
      361 agcgtctata taacaagcta ttagctgttt ctaatggtaa tggacagaaa ataggaataa
      421 ttatatgtga cttatatagg ttggctaaaa attagaaaga ttttgagata gcgaagtagc
      481 aaggtagtaa tatatttatc atattatatt acagttatat aatatcatta tttgaataaa
      541 tattcttttg tccttacggc ggaagcatga atccctacaa gttctgagaa aataactgcc
      601 tttgtaattt agtaactatt ttatacaatt tgcaaatttg ttgggcagga agaacttatg
      661 gcatcaggta tttagatcgt tataagttat atgccccata aaaattttct ccccagtaaa
      721 tgttatcagg gtgcaaatga gtccttactt aaatcattct ttatttcttc cagctacaag
      781 tgtttccgta agagaaaatt cctgtccgca aaaaacaagt tgtgaaatac cgttattttt
      841 tagagggtaa ttcaaaggtg gtttcagaaa gacaatatcc gcttcctttc ctatttccaa
      901 agaaccgatt atcttatcta caccaagaag tttggcacta cctaatgtta tatgataaag
      961 tgccttggca ggtaaaacag gataatcgct ttgacggtaa ttcatcatct ttgtatgata
     1021 gagcatattc aaagaggttc cggcacctac atcactacca agaccgtatt caatacctgc
     1081 ttcctcaatt ttttgcaaag ggaattctcc acttttcagg aaaaaattgg aatccggaca
     1141 atgcgctatt ttacatttat tttctgccaa catttttatt tcttcatcac caagatgaat
     1201 gcaatgggca aaaatactgt gttgagttag caaacctgct ttttgataaa cttcagtata
     1261 attcttaaac ccgaagattt ccttcaccat ctctatttct tctttgtttt cagaaagatg
     1321 ggtctgaatc caagcattat gttctattgc atatttagca acctctttca tcagttccag
     1381 tgaacaagtt ggagcaaaac gcggagtaaa aatataatcc agtttggcat ttttcccgtg
     1441 ccatttttca tagagcagaa tactttcttc cagtgtctta tggctgtttt gtggaagatt
     1501 ttccgggcag ttcatatcca tcatcgtcat tccgattaaa gctctaattc cagtttcttt
     1561 tgcaatttca aaagccatat cgcaggcaga aaaaaagggt gcggtataaa tgacgcaggt
     1621 tgtagtgcct gcctttagca ttgcagaaaa aaaatctcgg ctcagctttt cggcatattc
     1681 aatattttta gagcgttctt cttcaggaaa aacatattta ttcagccagg gtagcaaagc
     1741 cggttcgtaa agtccccgaa tataatattg gcttaaatgc gtgtgtaagt caataaaccc
     1801 gggcaaagca tattcgttag acctgtcttc atctattctc ccttgaaatt caggtaagga
     1861 tgtaatagca gatattttac tttcttcaat gctgataacc tgtttcggta aaaattgtat
     1921 ctcttcaggg gaaataggat taacgatgtt acaaaggatg tttttcattg ttcaactttc
     1981 ctgattttga ttactttagc tttgccaatg ccaaaatgca gttccggagc attctgagaa
     2041 ccggttcctt tggcactgca aagttcccgt ataaattttt gttttttatg aaaaagccgt
     2101 tttactgtta tttctaccct ggtgccaaag gcaggactat taggaacttt ggtaaaattc
     2161 tctgcattat tttccagtaa gacaatatcg tttttccaat agggcttaat aaaaagacct
     2221 ttattctttg ttattgtccc attatgcaat atcttagatc cattgccact gcctacaata
     2281 aggtcctgta aaccatctct atctaaatct gcacaggcat ttccccaacc gttataaacc
     2341 ctggctccag caagccaggt tacatcttca aaagtgccat cgcctttatt atgatagaga
     2401 tagctccgtt cattctcata aacggaagtg ataaacaaat ccaggaagcc gtcattatca
     2461 gcatcaaaga aaagaggatc gctatgcaat tcgtcgtatt ttattcctgc ctgctcggtt
     2521 acatcggtaa attgccaata gtaaatagtg tctttttcca caatcctata agttaagcca
     2581 tcattcctca ggagcatact gatatcggaa atatctatat aacggggatg agcaaggtta
     2641 gcaacaaaca aatccaaatc gccatcatta tcataatcac cccagtccgc tccaatgctg
     2701 tgaccataat atccattttt atatgttcct gctatgccgt aaagagcggc tacatcaaca
     2761 aaaagagtat ctatctgttg ccagcagaaa ttacggctca aacggtagtt ggttacatat
     2821 atttcctgtt ttccatcgtt attaaaatca gcaggggcaa ctcctcttcc gggtaaaccg
     2881 ggattatcgg tataaggagg ttcgcgaaag ccgtaggcat cacttttatc cgtaaaatag
     2941 ccaccatcgt ttttccagaa ataatcggga aaaccgctcc gcttcagcca gatttcataa
     3001 ttggcagaat agagtgaagg gtaacctttt tggtcaatat caatccaggc aacagcttct
     3061 gtaggataag tatcatcaat atctccggct ctttcattca cattcacaaa ccttatatta
     3121 tccatatttt tcagcaactg gtcacctctt ccttcagcag aatggctata ggaaaccaaa
     3181 tctaaaagtc cgtctttatt aaaatcggca aaaatgcctc cattgctact caaattagcc
     3241 aaaccagcac tgtccgttag ctctgtaaac ttcatcttcc cttcatttct ataaaggtat
     3301 ttgccgttga aaagaatatc cgtaaaaccg tctgaattat agtccgccaa agcaactcgt
     3361 gtatatcctt tgccgttaag acccgcattt tcgcttttat cttcaaagca gattccctta
     3421 tagttcatca ttttgcgcat ccattttagc ggttcttctt ttattttaag gtcggtatgc
     3481 agttttgtta tcagtgccat tgcttctaca tcatatctat tacggggtga acctgaaatc
     3541 aaacattggg taagatgttc tgccgctttt tgtttatctg ttttctttcc tgtcagtagt
     3601 aatgctttag ctgtccaaaa atgtttttct gcttgttcgc cacgattatt gttactgaat
     3661 tgaacttgag caagtaatgc aagtaattcc tgttgttgtt tcagcttatt tttctccagc
     3721 atcgggatat ccaattcgtc accaaaaagt ccgtagggct caattatcct ataaaacagc
     3781 aattttgcct gttgttgtaa aacccttgct ttccaatcat cagcactata aatatcataa
     3841 agcaaggttt gttcgcaatc ggacaaagcc aatgttaaat attgttctgc cagttccagc
     3901 aggttttctt caccttcaaa atcctttctg aaagtggggg agagcaaaaa cagagaagca
     3961 atatatgcgg aaacaggatc taaattagtg tgttggggta gtgcctttat cagttcttgc
     4021 caatttttac ggttggcaaa atgataaagt ttatagtata aagcaataga acgatagcgg
     4081 gaatggggat atttcgtttc aaattcattc aacagattaa aggcgagaga gtcgttgcgt
     4141 tcaatactga tttcatcaat caaatccttt gccattgctt caatgattgt gttatacttt
     4201 ttggttgtgg caattttagc atatagctct tcttcactgc atctttttgt gtagtaaagc
     4261 caaacagcac tatccaaaga atcggttaat gcttccgtga tagcactaaa atcattatag
     4321 aaggttaagg aatcaacttc ttccgtttca cagagcatca gccactgtaa agcatcttcc
     4381 aaagagttat aatttagcgc taaacgctta tggcattgtt ttgccaattc tggtaagttt
     4441 gctctatggg aataattcaa aaccgtttgc aggtcttctt catctccata taaaactttt
     4501 gcctgaattg tttcgtcgta tttcgcaaga atctgaaatt gttccttatc gcataaaaaa
     4561 ttgatttctt cattggtaat ggcataacag ccgaagacaa gtagaataaa gataaacaaa
     4621 ttgatatatc tcatttttgt tcctgcagtt gttcaattaa gctctggata tcttcagagc
     4681 taaaattata ttccttattg caatagaggc aaacgggttt gatgccttcc tttagtgatt
     4741 gcagttcctc ttttcctaaa agcaataatg ccttgctgaa tagttcatag ctgcaattac
     4801 aacgataggc gatttctttt tcatcgttaa tttgccactg aagtcccttg aacacaaaaa
     4861 gggaaaggat ttcgggaatt gataatccca tatccatcaa gtcgctgaga ttgggagttt
     4921 gtgctatatt ttcattaatc acatcagcta tttttttatc cgcataaggt agctgttgaa
     4981 taataaaacc tcctgcagag cgcacttttg cttctttatc aattaagatt cccaaattta
     5041 ccgctgtagg tatttgttct gattgttgat agtagtaagc caggtcttcg gcaatttctc
     5101 cgttgataag ttctatagtg ctaaaataag atcttgttcc ttttttatct ttgctgatag
     5161 ttaaagttcc ttttccgact gctttgccta ccttaaagtt atcttctctg gttgcataaa
     5221 agaggcgggg attttttgta tagccacgca gaaagccatt ttgcgtgcag attacaattg
     5281 caccttgaat atcaccttcg caatctattc tcattgtaat ttcactttct tcatctttca
     5341 agtcccagga aagcattgct gctgctgaaa tcattcttcc taaaagcaaa gtggcaattg
     5401 gagaaaggtc atgtaaatcg cgagcggttt gcacacaatt ggtactatta acggcaaaaa
     5461 cccgaaaatt attgttatga actgtcccgc gtaaaatgta atccttagtc atcaatccaa
     5521 tccttgttta ttgttgttcc cggataatag tgctgtaaaa tttcctcata ttttgttcct
     5581 tttcttgcca ttcgcaaagc tccaacctga cacattccaa ctccatgacc tgcacctctg
     5641 cctttaattt ttatatttgg cttggggata attattacag taccattagc aatttggtaa
     5701 gaaccttcaa tataaaagaa tgacgaaagc aggtttccga aaacttggcg gatttgatat
     5761 tcgctattta agctaattgt ctcatttccg atcagtttta ctttagtgat gcgtccggaa
     5821 gagccgcgct gaagaatttc aattctgctg atggaactta aacctgcatt ttttgacaat
     5881 tgattaagcg aaatggattt atgccaggaa agcgcaccgc gttcccagga actcatttct
     5941 tcttctgcca aaggtttatc tatccacgcg cgagcatctt tttctttgct taaatcaaaa
     6001 gaattaactg cattgagaca ggtgacacct tttaaataat caatcggttt acctttccag
     6061 ataatatcag ctgcttccgt ttttcctcca cagcaactat gataggttgt ttcagcaaga
     6121 tttccgttat aaaacaaaac ctcagaagca cattcataaa cggcttgcag gatttgttca
     6181 ttcagcaaat attttccttt gtaaacctgg cagtgagtgg aattgcataa atcgtaacca
     6241 tcggttttat gtttgttatt caatagcaaa gttagggcat gagttcttgc tgcaactatt
     6301 tgtgctttaa gtgcttctgc gggagcattg tcaccaattt cattttgcac tactccggca
     6361 agataggttt ctaaaggcaa taattgattc aagataatct gtttttttac ggtcttgaga
     6421 ataaaatcgc cactgtaatc atagctatct cggttaaaat agatttctcc ctcggaatga
     6481 attttcaagg gtgtttccat atagcaaaga tttcccgtag catcttgaat ctgaacagtg
     6541 gaattaagca attgcacttc tttgatttta tctataggaa tgccctgtat agaggcatat
     6601 tgttttgcct cttcttccgt agtaaaactc tccggcagaa agtgttgaag ctccttttta
     6661 attttcaggc gggaattttc ccaggcaaaa aaatctcgga ctaccgcttg ttcatcatcc
     6721 tgggctgcat aaacaatctc tgtaaagtcc agaatagcat aatggacaag tgtttccgga
     6781 ttcataatgt taattgtgat agcggaatca aaattgcggg atattagtcc atccatttcc
     6841 gctatagtta tattggcatt tttccctttg ggagtaatag ttagttgagg agaagcagtt
     6901 aaattgattt ccaggtagag ttcacctttt ataaattgcg ctccggcaag caatttaaga
     6961 caaagtaaca gcaaaattat caggtagtta cgcttcattt ctctttatga tatcaaaaag
     7021 aggttgttat tcctgtggaa gcaagaatcc agataaaatt gagtaaaata aacacatttt
     7081 caagatgaat acgatataaa ttattgaaca actgcaccag gaattccttc tccgtcagga
     7141 attaaaagca gtaattttcc gttggaatcg gcagccagaa tcattccctg cgaagttatt
     7201 cctctaattt ttcgcggttc aagatttacc agcattaaaa cggttttgcc aattaaatta
     7261 tttggttcgt aatcaactgc aattccggca atcaattcgc gttgttcaat gccgatatct
     7321 acctttaatt ttaacagttt atccgtttga ggaactcttt ctgcggttaa tactttggct
     7381 aagcgcaaat ccaatttggc aaagtcatca taactgatta actctttcag cggagctatt
     7441 tccggagtgt taaaatccga tttgtcacct tcatttatta ttgcttgtga atgcagtttg
     7501 gcaatctgtg cctcaatttc tttatcctct attttccgga aaagcggttt gatttcgttt
     7561 aaaacgatat ctcccaataa attatatgct tcgctgaact tgcaattatc atccaaattc
     7621 atcatctgcc ttaaataccg catagacctt ggcataattg gactaaaggc aacggatatt
     7681 tttgccagta aattagagca aacataaagt gtttcgttag ttgcatctct attttctttc
     7741 agctgtgccc agggcttgtt ttcgtcaaaa tagcggtttc ccaaccgagc tatatccatt
     7801 atcagacgcg tatttttctt cacttgatag tccagataat tttcctcaat ttcttttaag
     7861 agcttatctg cttcagctat tgccatatta gccgtttcac ttaaagcgca ggcagttatt
     7921 ttaccctcaa aattcttgtt agcaaaggca aaaacccggt tggcaaggtt gcccaaagta
     7981 ttattcagct ctatatttat cttattttga aaatccttaa aactaaaatc ggaatcttgt
     8041 ctttcgggag cgttaactgc caaataataa cgcagatact cactgtcaaa atcctgaatg
     8101 aattcgtcta cccaaattgc ccaattctta ctggtagata tttttttccc ttccagattc
     8161 ataaattcat tggcaggaat atcataaggc aggcaatagg tagtatcctg tcccatcagc
     8221 attgagggcc aaataagcga atgaaagata atattatcct ttccgataaa atgaattaaa
     8281 cgggtttcgg gatttaacca atattccttc catttttccg gttgacctat tttttctgcc
     8341 cattccacag tagctgaaat atatcctatg ggagcgtcaa accaaacata aagaactttg
     8401 ccttttgctt cgggaagagg aacgggaact ccccaggata aatccctggt aacggagcgt
     8461 tcaactaaac cctggtcaag caaattcaga ataaaattcc gcacattttc tttccaatag
     8521 tcctttgtgg caagccagcg tttaagttct tcgcgaaatc cttccagtag caaaaaccag
     8581 tgtttggtct ctttaattac gggagttgaa ccgcatattt tacatttcgg ttcaatcaag
     8641 gttgtagttt cgtaaatttg accgcatttg tcacactgat cacctcttgc atcttcaaaa
     8701 ccgcaaaggg gacaaattcc ttccacatat ctatcgggaa gatagcgttt atctttttca
     8761 cagtaaaact ggagcgtgtt tttgggtttg atataaccct tattgtataa ttgcagaaag
     8821 aagtcagcag caagtttata atgaggggga cgggaagttc cgctgaaatt atcaaattca
     8881 ataccaatac cctcaaatgc ttttttaata ctattatgat aatagtttac tacttcctga
     8941 ggtgtaattc cttctttatc agctgttatg gatattggag tgccatgttc atccgttcca
     9001 caaatgtaaa taacatcttc acccttcaaa cgcaaatagc gaacaaaaat atctgctggt
     9061 aaataagccc cggctaaatg tccgatatgc aattttccat tagcgtaagg aagggcgctg
     9121 gtaactaaat atttcatacc tgttcctcaa cataaatttt atgcagggtt tttaccgctg
     9181 cggcacaatt ttggtctggc agtagaagct ctatgctttg ttcgttatta aagctccgca
     9241 ggatgttaaa attttggcag ttttccatta cggttgccaa aaaagcaggg tcgtgaccta
     9301 agcccaatcc gataagattt acaaaggcaa aatcgccttt tttgctgatt ggctttatat
     9361 ctctttcctg cagaaaatag tccacttccg tttcatattt gctttctaca aaaagttcca
     9421 gaagaccttc attgacaaag cttttataaa tttcataata ccagtcgcta agagttttta
     9481 gcactttgtc attaagcggg aaagtatatc tcagtaatgt atttttatga gcaatagcag
     9541 ttaccttgcg ttcttccata ttattttcct cattgttttc tgtcttttta ttggttatca
     9601 gcgaaccggg agcaaaagtg aaagatgatt ttacttctac ttccacaccg tatttaagag
     9661 cgtattccgc agctcgggga tgcaagacct tagagccatt gtaacaaaga gttaacatca
     9721 gctccgggct gatttgtctt aataatttcg gcttttccac aagcttggga tcagcagtaa
     9781 aaactccgtc cacatctgta taaatttcgc atttatttgc ctttaaataa caagctaaag
     9841 caactgcaga agtatcagaa ccaccacgtc ctaaagtagt tatttcttta ctggtgctaa
     9901 caccttgaaa acccgctaca ataactactt ttcccttttc cagttcttcc tgtatacgaa
     9961 aggcattcac tttcaaaatc cgggcattac catgcttttc atcggttatt attccgcttt
    10021 gagaaccagt aaatgaaata gagggaattc cttcttccat tagggctaac gacaataaac
    10081 tcatactgat ccgttcacca gcagtcaata acatatccaa ttcccgacgc gaaggatgct
    10141 tggaaatccc gtaagctaaa gaaaatagtt catccgttgt tttagccatc gccgaaacaa
    10201 ctaaaacaag accttctcct ttgtggtatt cactggttaa tctatgagct atgttacgga
    10261 ttaaatcaat gttaccaact gaagttccac cgaatttttt cactattatt gccatatctt
    10321 tctttatttc aagatttgta gttgctgtca aggaaaaaaa tctcgctttc ttaatagcat
    10381 ttctttagca ttacggactc attgctaatg ccattagtaa tgggtaagca atgagtaagc
    10441 aatgctatta aggaagaaaa tggaattata tttatttagt aggagttagg agaattcctg
    10501 ctttctgttt taactcacaa ttttttattt gggaaactaa ctatagcgcg attggaagta
    10561 aaaaaacaag aggagtgttt ataattggga aagcaggtga aaattgtaag tcaaaacttt
    10621 ctcgcaggtg aaaattgtga gtcaaaacct tctaaatcat aaattttcca agttttctct
    10681 ggtttcccta ttaaattcac tcttcatttc aagaggagca ttttttttat gcaggaatag
    10741 tttttacttt ccagccggta gaaatacatt ccggaagcca cattgcggtt gttattatct
    10801 tttccgttcc agacaatttg atgcattccc gaaggtaaaa cttcatctat cagctttttc
    10861 accagttgtc ctttaatgtt atatacataa agtttagcgg gagccgtttc ctgcaaagta
    10921 aatctgatag ttgtttccgg attgaaagga ttaggataat tatttaacaa ttgagttata
    10981 agagcaggat tcacattatc ttcaatatct gtaagataat tgtagtgcaa aatctctgaa
    11041 ggcatactgc taccctccga ataaacgcaa accacataaa aataataatc cgtgcctaat
    11101 tctcttaatg tttccgtata aacaggtata actgtaattc ctgccaatgc aaaagctccg
    11161 gcattaattc tacgataaat ttcatagtgt attaaaggaa attccggttc agaaggggga
    11221 ttccagttaa gagttaaaac gctatcggct aacatataac taattcccga aacgggagta
    11281 aaataaccca gataaaagtc ctgctgaact aaagggttag ccagagaaat tgtgatcaca
    11341 gcagaagtag aatttttata gccatctgca gaagcccaaa ttgaatgcga gccaataggg
    11401 agataaattg cgtagtttcc ttcattatca ggatgactaa tccaggaagc agaattttgc
    11461 acattaacta tgctgaaatc aatagcagaa ttactgctgg ttaccgttcc gaaaaccttt
    11521 cccgcaaatt caacaaaacc tgtagtgcga acatcatcta taaaccatcc atctcctgta
    11581 ataccactat cactcgcaaa agtaaaacgg aattgaacac tctgattggc ataaattgaa
    11641 agattgaaac gggcaaaaac ccagccacca ctgtttccac tataacctgg tccgtttaac
    11701 acactcaaat ttgtgtttga ataaccacct accggagtaa gcaaagtcca ggttgttccc
    11761 ccgtttgtag aaattttaac attgccccca tcataatttg cttctgaatt ataataatgc
    11821 cagaattcca gcataaaatt gctgccgata taaacactcg gagtagtaag ctggtaatta
    11881 gcattattag gataagtact attcaatctt gttccccaga ccttagttcc ggaatgagct
    11941 cctgcaacag ttgattcacc ccattcccaa ccgttggatg aaggattggg aacaaaattt
    12001 ccgttattat tttcaaaatc ggcattcata cctgtagtgc ctacacttaa aaccaattgt
    12061 tcactatgaa cacttgtaaa atcagccaaa tatgttagat agaaagtaag attgttccca
    12121 actatcacat tcggagaaat agttatatta tatattgctt ggcttattcc ccctgcaggt
    12181 aaggaagaaa taatgcagga attgttatga acagtaacat attccgaaag gcaagtgatt
    12241 gtgctgctaa tattattagc agaaacagga gtgttattgg caaaattgat aatcaaatca
    12301 agtgtttcac ccggatcaat aagtccgttt ccatttcctg taatactatc gttcatatac
    12361 attccactat attgtatatc aggtttatga acagtaatat aaacagtaaa atcccagctg
    12421 ttatctgaaa tgctgatatg caagttgatt gggatttcag ctgcattggg acaattggca
    12481 tcaatttgaa gagtaattgg ggtattattt atcgctgttg catctcctaa aatattactg
    12541 taattgctga tagcattgat cagaacggca tattcgcttg cacaaaaagc acttaactga
    12601 acttgtgtag ctgtgcctaa tcctatattc attagtttaa tacccagttc aattgtttct
    12661 cccggttcgg ctatctgatt aaagttggta tcatttataa ttacttcatt caataccaga
    12721 aaaggtgatt cgtatagaac tggagctgta gtaatcaaca gagctgaaaa atttgttatg
    12781 ggagctgctg cactgggata ttgattgtta aaagtatatt ccagccctat agtattcgta
    12841 tgatctttaa ttccgatagt gcagaaattg ccatgcgaag gactgtatcc tccctgcccg
    12901 atatccacat tattgaaatc ctgatattga aatttaacca ttccctgtcc cattgaggta
    12961 ggatagaaaa cgggatcata aaaaataact tcaaaggtct ccagagaaga acgattgtat
    13021 ccattacgca ttttgaaata ttctataata aacctgtgat tttggctatc gtaatattta
    13081 tatatccctg catcattaat taaacataaa tcatcccaga aaggagcaag catgggagaa
    13141 ggtccgtaac cacctggaag atgatagttc cgaaattcgc cattttccgt aaaccccatt
    13201 actataaagc catttacgca aacggtaatt tgattgtagt caatgccata aaagggaaaa
    13261 gtgaaaggca aatctatttg ctgtaaagtg accgctccgg tttgatctcc ttcgtcagtt
    13321 gaagaaccgg gatcggaaaa agcggttatt tttgttcccg ctcctcccaa ggaaggattg
    13381 atttctatcc attcatacac aggacaatca ggataagcgg tatcggttat atcataaata
    13441 aaatagccga aagcatcggg acctaaaggt gtgtgagaat taacactacc tatttgaatg
    13501 ttaaactcgg tatattgatc aaagccccat tcgttatata ggtgtaaacg caacggcatt
    13561 tgcattccag gaatcaataa aggccgggca aagacctcaa aaccttctac agtagtcccc
    13621 aaactgtttc ctaaaatgga acccacataa gaagtagaat ccgtaacggt tatcagatcg
    13681 ttcaaagaag ataattcggc ataaacatca aagacaggag caatggaatt atttttaatg
    13741 gttaaggaaa gaaaaccatt ttcacccgga tcaagaatat tatttcctcc tgcagatatt
    13801 gaagagttgt gaacaagcaa caaagcatta tagataacag tatgaaatac aaaggaataa
    13861 acattttgat cagttgttgt taattgcaat gtaaaacgca cattatgctg agcaggtaaa
    13921 ttgtttacta tgctgaaagt aaatacagaa ttagaccaaa gcgtggcatt actgtcaata
    13981 gcgtcaaaac tgctttcaga attcaaaata gtaatataag gatcatctgc agaaagaatg
    14041 gctgttgttg ctggaactgt tgctgaagtt gtatttttaa tttccaaact tagagctatg
    14101 gtttctcctg cagtggcaaa actatcgcca ttaccgtaac tgccattagt gccgttatca
    14161 ataactattt tgtttaaata aacaagagaa cctgtagggt caattgttac aactttttgg
    14221 aagggtttat aattatgttt ggaaacggta accgtcagct catttagcat cccgcctgaa
    14281 atattgatag ttgccttacc ttcactgtcg gtataatctc tgccaactat aatattctgg
    14341 gcagtactat aaagtgttac acaggcatct tttactcccg tcccaaagga atttgtaact
    14401 tccacatcca aaagattact gcctaaaggt aaaatatctg cacaagcgag gttaaaaata
    14461 tcaggaattt gagtaaaaac ctctaccgta gggtctccca ttaaattaca ccaatgggca
    14521 aaataattcg cctgttgatc aaaagtagaa ccataaacct gcttaatgta taatttggca
    14581 tttaacaatg cttctcccat actgcgcata ccataagtaa aaatcccgtc ataaattccg
    14641 gcattcaaac aattgttaaa agtagtatga gttccactag tagccattcc aattgctgta
    14701 attgctccag caggagctgc agaagttcct aaacgaataa gagattcgga tgtagcggtg
    14761 ttattttcaa aattaccagt gctacaagta agaattgtag aatgaggtag cttattgcca
    14821 ttgattaaac tggcggatgg actccaaccg ctcattccta accaaccgcg ataattgaaa
    14881 aaggcaactc cctgattaaa accattgtca atataggcag ggaatccgct ggaataattc
    14941 tccagaaaag tataatcggg attagttctt tttgccaatt ccttgatgaa tttatttaca
    15001 taaatggtag aaattccact gttaccaggg tctccaatca gcaacatttt atttacccag
    15061 gcacctgcgt ttccggatac attgatattc ttttccacag cataaatttt agtaaaaagc
    15121 acatccaatt gactgatatt ttctgctgaa attctaccga taaaagcgtc tcccaaataa
    15181 tcgttacctg ctaaaaaagt ataaggataa tcccctttac ctccataagt ggacatttgt
    15241 tcagtatatg taggaatggc ataacttccg ttagtatcac ctaaaaggat aataaaatcg
    15301 ggacgcgtag aaggattatc atattgcgtt tggatatagt tcttaattgc ctgagtagaa
    15361 gctcctccgg tttcaactgt gcttacaaaa tttacatcat agcccttttg ccttttccag
    15421 gcaacaaatt cattcagttt ggcaatgaaa atagcatccg tattgtttcc gtaaataagt
    15481 aatattcttg caggttgagg agccatcatc gtattccggt aataatcaaa attggcaatc
    15541 atagattcat aaagattggt gaaggcataa gaatagccgt tatattcatc catttcattg
    15601 attccctgtc tgtccttaac ctctatttca acttccatct gtttttggat tttcagttct
    15661 tgcgtttctg ctacatattg aaccggatta agagtaatct gcacaaccct gaaatcacgg
    15721 ataatttggg gttgactata gctgtaacta tttgcaggat ataaggaagc actatgataa
    15781 gctgttttgt tatattcgct tgcaatttcc tgttcttttg tggcaaaaac cggtttagga
    15841 ataaacccct tttgggtaat aatctctcct ccggtcactt ttatttctat atctcctttt
    15901 ggaggaatag caatccaagc agaaaaaacg ggcaattcag gaaaccctgt ttccgctgtt
    15961 gtttctgcac cttgcatact caggatttta aagttgctgt tatcaatatc ttcaatttgc
    16021 agttcgggaa cctgtaattg cagttttatc tttcctgcat tctgagaaac cacggaaagg
    16081 ggtttttcta tcggaataat tgttcctgca actaaggcac tgaatgaaca catcaggatt
    16141 agaatggcga ttttagttgt tttcatcttc cgtaaacctt tatttggtat aaattaaaaa
    16201 gaacctgaag ttaaatttac tctaacttca ggttctttcc cattgtaaga ttgtctcata
    16261 aattacttta tctcgtagat aagaatgccc tgatcgcgag ctcctactac taatttatta
    16321 tccataaatt tcacagtatt ggtgtaaccg caggaagtta aatgctgtaa caattgcggt
    16381 tttgaaggaa tactaacatc aaaaagataa atacctcctc ctccggaaga aagagcaagt
    16441 ttatttcctg aaacagctaa ggaagtagca taaccacttg tatcaaatgt tgacagcaac
    16501 actggtgaag caggattgct tacatcaaca atatgtaaac cgctttggcg tcctgctaca
    16561 taagcaatat tgttattaac ttccactttt tgagcttctc cacttaaatg aatattgcca
    16621 atcagctgac ggtcattacg attataaata tataaaccaa gctgttctgc ggctatgaaa
    16681 atgtaattat tggtcaattt aaaaccagca gctgctccag gagaaatggt aaacacatta
    16741 ctgttgaaaa ttgtgccatc ataataatca tatttgaaac ttcctgctgc acaataggca
    16801 caaaccattt tataagttcc ctgtaaaaaa ggaacaggag ttatggcaaa actatccaaa
    16861 tcctttatat tacttgttcc acctataaaa tcaagaatgt aaattaggga atcgggatcg
    16921 ctggtattaa ttactctaat agcatcagtt gcctcggtat ccactaaaaa cataaaatta
    16981 tgttccggta ctaccgctac ctttttaatt ctgcctaaaa ctttctggct accatcttct
    17041 gcccaaaatt cagtaatcca ctgtatatga taatccgttc tactaattcg ggacattcca
    17101 ccctggtcat gtgccacata tatataatta tcatcaatct tgatatccaa aggattgcct
    17161 acaatcggta aaacatttct caaagtaagc caatctcctt cctgatattc agtattgcgt
    17221 tttgcgcatg aacttagcaa tattagcacg atgattgcta atgttattat ataactttta
    17281 ggcattgagt tcctccactg ccttttttca tctttcctat agaataatgc aaaaatcatt
    17341 ccgaaataaa atatgcttta cattatgata ggacatataa agagaaatgt caggtttgta
    17401 acttttttat agattgccaa tgaataaaag cccagcgcta tcattgtatc gttcttcgga
    17461 tttttgtggt tgaatgtttc atagtgttgt tcagattaaa aaggtattgt taggcatttg
    17521 gcatatatta tatcattttg aatgttaagc catttttcat tcctgcgcaa gcagaaatca
    17581 ttaaaaacag aaagagctct ctgcttcttt tcaagagagc tcttttttag gattagggtt
    17641 ggagatttct atgccaacat ttttgctata tcgctgtccg catcggtaat ttgttgcaaa
    17701 ccaaaggtat cagaaataac ttttgccaca ttgggagaaa gaaatccggg caaagttgga
    17761 cctaaaataa tattcttaaa tcccaaagaa agtaaagcca gaagaacagc tacggctttt
    17821 tgttcatacc aggcaagatc aaaagaaaga ggaagtttat ttacatcatc caaaccaaat
    17881 gcctctttca gtttcaaagc aataactgcc agcgaatagg aatcattgca ttgtccggca
    17941 tccaaaaccc ttgggattcc attaatatca cctaaaggaa gttttatata acgatatttg
    18001 gcacatccgg ctgtaagaat aaccgtatct ttaggcaatt tttccgctac ttcagtaaaa
    18061 tatttcctgc ttggcattct tccatcacaa ccagccatca caacaaagcg ttttatagct
    18121 ccacttttaa ctgcttcaat gattttatcc gccaagctta aaacagtgga atgagcaaaa
    18181 ccgccggtaa tttcaccagt ttcaagttct tttggaggtt ggcattttag agctcttgca
    18241 attacagggc taaaatcctt ttctctgcct ttttccctat ccggaatatg cactgctcca
    18301 ggataattta ctgttcctgt agtaaataaa cgctccaaat aggtattttc cttgcgcaag
    18361 ggaacaaggc aattcgtagt cattaaaata gctccgttaa agttttcaaa gtcctccagt
    18421 tgatgccacc aggaatcacc ataattaccg ataaagtgtt tgtatttctt aaaagcggga
    18481 taataatgag caggcagcat ttctccgtga gtgtatatat caacaccctt gccttcggtt
    18541 tgtttcagaa gttcttccaa atccttcaaa tcgtgaccgc taattaaaat tccggggttc
    18601 ttacctactc ccagttttac ttttgtgggt tccggattgc cgtaggtttt ggtatttgcc
    18661 tcatccagtt ttgccataac tttaacggca ttttctcctg tccgcaaaac caaatttacc
    18721 agctgatcaa cagttaaatt atcatctaaa gtggcggaca gaccttccat aatgaacttg
    18781 tttacatcat catcctgaaa acctaaaatg gcagcgtgtt caccgtaagc gcaaatacct
    18841 tttatgccgt aaataattgt ttctcgtaaa gaacggatgt cctcattttc tgttctaagc
    18901 acaccaactt ttttggcaaa ttcatcatat tcatctcttt tcagctgaaa agtaactgct
    18961 tcgggacagg tatcacattt accttccatc aatttacaga gctcatcttt ccgcttatcc
    19021 ctcacagcca aagttgtttc aattgtgtct ttgatgtttt cttcgtccca attgacatta
    19081 gtaatagttt taaataaagc gctcatcaca aaaacggaat cttcaggata atatctatcc
    19141 ttttcctgca gtttcacgga tacataagcg agacccttca atccgtaaat caacagatca
    19201 aacaaattgg caagtgtttc actttttccg caaacaccac gcattgtgca tcccaggtta
    19261 cgagatgttt cctgacattg ataacagaac atactcattt tattttctcc ttttttaggg
    19321 ttttggcatt ttaattacca ataatcttaa atttgaattt ccttcattga atacaccgtg
    19381 aggaatatcc tttggacttt caatcagagt gtcttcagga aaactctgtt tctcttctcc
    19441 aatttccaaa gtggctgttc cttccaatac atagaaagcc acatttacag gagtgatatg
    19501 tttctttaaa tgagctcctg gtgctaaatt aagatgaact atctctcctt cgggtgcttc
    19561 gtaaattttg gaacccaaaa taccattggc atttactttg ggttccacat cctgccaggt
    19621 tctgcttttc atttttcctc cttattgttt tgtttctatt agttttatta ttgtttctat
    19681 cagttctgaa ccttctttct tcagatcaaa agcgccatca ctcaaattcc actgagtaac
    19741 tgtaaatctc attgaaccca caataattct gaataattga gttggagaaa ggtcacttcg
    19801 gattttgcct tcctgttgtg cctgaatgat ataattttcc attacctgct tgtgtttatg
    19861 cataatacac tgaaactgac ctttaaaggt aggatcattt ttaaatagct cttcggaaaa
    19921 catcactttt gccaggttgg gatgctcact gaaaaggata tacctatcct ccacaaaatg
    19981 cctgatactt tcaagtggag aataatcttt ttctcttatt tcctgtaaaa ccttatttga
    20041 aagttcctca aaataaccta aaatcgttgt cactaaatcc cttttacttt taaaatgtct
    20101 gtaaagtgct gcttctgtaa cccctatttt agttgccaga ttctttgtag ttagcttttc
    20161 ataaccttga ttagcaatta tagcaattcc agccattatt atatcttttt gtctttcagt
    20221 tagttccatc cttcttccct tatgtaagta agtattcact aactaaaata atcaagcccg
    20281 cataaatgtc aagaaaaaag taaggaaagt tgagaagtca agagatagag aggatacctt
    20341 ctatttgtgt agccggagga tgcggttcct actctgcaaa aaaaatgcag agctacagcg
    20401 agttctgaca acacattttc cagtttgtaa cttaaagaaa aaaataaatc agctttggtg
    20461 tcctcagtaa cctcattgtg cttaatgtaa ataaatctgc gtcagttatt ttttaattgt
    20521 taatccaagc atatcacttt agtatattat tagaggaata tagtatgcga ccggataaaa
    20581 ataaactaat agaactgctt tccaccgaag gggaagagaa ccttcaaaca ttatataatc
    20641 aggcatacaa ggttaaaaaa aagtatgtgg gcactaccgt atacctgcgt ggtttaattg
    20701 agcttagtaa tatctgttcc aagaattgtt attattgtgg aatccgtagt ggcaataaag
    20761 aacttaagcg ttatcagatc agttatgaag aggcaattgc agaggcaaaa ttatgccaac
    20821 aaatgcatta tggttcaatt gttattcagg caggggaaag aaacgataaa ccgtggattg
    20881 atttcatcac caagctcata atagggatca aagaactatc tgaaggtaaa ttaggaatta
    20941 ctttatccct tggagaacaa aagcgagagg tctatcagca atggtttgaa gcaggagcac
    21001 atcgttatct tttacgcatt gagacctcca atgaagagtt atacaaatca ttacatccag
    21061 ctgctcaaag ttttgaaaca cgtttaaaat gcttgcattc aataaaagaa tgcggttatc
    21121 aagttggcac aggagttatg attggtttac cgggacaaac aattgaggat ttagccaatg
    21181 atatactatt tttctatgat gaagatatag atatggtagg aatgggaccc tatatccctc
    21241 atcataatac acctttaaag catttggtag atagttttaa tcctgcagag gcattaaaaa
    21301 tgggacttaa aatgattgct gcgtgtcgca tagctctaaa ggatgttaat attgcttcta
    21361 ctactgcttt gcaagcgcta catccggaag gtagagaatt ggggttaaaa gcaggtgcca
    21421 atgttttaat gcctaatata actgatacaa aatacagaaa gggttatcaa ctttacgagg
    21481 ggaaacctgg cttgaatgaa actgctattc aatatcaaaa ggaattggaa caacgcattt
    21541 ctgcccttgg agaaacaatt ggctacgatg aatgggggga ttcaaaacat ttttacagcc
    21601 gagaagtgca gaggtcaatg aagaatgaaa aatgaagata gctccgaaac cataaagaaa
    21661 gctcaatgtt ctatgtgttt tcggtgttct tggtgtaaat aaatcttttc cttgacggat
    21721 acaccttatt ccaagaataa gcacaatact ataagaaaag gagttttata tgcttcccgg
    21781 tggaaaaaat atgcagcaat tgatgaaaca agctcaaaaa atgcaggcag aaataatgaa
    21841 acaacaagaa gaactggcga atacagtttt ttccgcttct tcgggtggag gaatggtaac
    21901 tgttgaaatg aatggacagt ttgaagttgt ttccttaaaa atagaccctc aagttgttga
    21961 tccggaagat gtagaaatgc tggaagacct tattactgca gcagttcagg aagttctaaa
    22021 caaggtgaat gaagcatcca gcaatgtaat gagtaaagtt acaggcggaa ttaatattcc
    22081 cggtttgggt taaatgcttt tttccgaaaa cttagaaaaa ctaattcaga gctttaaccg
    22141 ttttcctggt attggacgca aaaccgctca acgacttgcc tggtatctgg ttgcgcagga
    22201 taaaaacttt gctttaaacc tggcggaaac aataaaaaca accgttgaga gttttaaaac
    22261 ctgcagtcaa tgtaatatgc tttcagaaag cgatccctgt cctttttgca tttctccaga
    22321 ccgttccgac agttatcttt gcgtggtaga aaacagttcc gacatccaga tattagaagg
    22381 tatgaacgaa tttaaaggca ggtattttgt tttagggcat ttgctttccc ctttggatgg
    22441 ctacggacct gaacaaataa acacggctat gttgttggag agaatagaaa atcttcatcc
    22501 aggcgaagtt attttagctc taaaaccctc tgcggaaggt gaagcaacaa ttcactatct
    22561 ttgggaattg ctaaaagata aaggaatcag cataacccgc ctttcaaccg gaattccttt
    22621 cggaggagat ctggaataca gtaacttaaa tactttagcc aatgcctgga aacgcagata
    22681 taccgttcag taatatgttt accggaatta ttgaagcgac gggaaaaata gttactcacc
    22741 agctaataag cggaaaaaag ctgataacaa tttcccgacc cgctattttt gatgacctga
    22801 aaattggttg cagtattgct tgtgatggaa tttgccttac agttgttcag tttgatgaca
    22861 aggcatttac agtggaaata atgaacgaga cactccaaaa aagcacagct aaaaactgga
    22921 cttcaggtaa aattttaaat ctggaaagag cattaaaact gaattctcgt ctggatggac
    22981 actttgttca gggtcacata gatagagaat tgcgcttgat ttcacatctg aaaagagaag
    23041 gaaccgatta tctgcatttg gaacttccca gtcaggaaag aaatctaatt gttcctcaag
    23101 gtgcaattgc tctgaatgga gtcagtttaa ccatagcgga aattcgcaat aattatttta
    23161 gtgttgcctt aattggtcat actttaacgc atacaaatct gggaacaata aaaactggcg
    23221 aaaaggtaaa tgtggagtat gatatcttag gcaagtatgt tttagcgcag ataccgaaag
    23281 gttaatcaaa aaaagataaa attgttttac cccaaagggt taaagtttat gagatttaat
    23341 aagaccttta tctgcctctt ctttctgctg attatactat ttctaatcag cggttgtggc
    23401 aataaacgca gtcctacagg cggtcctcaa gatattgata agccagtagt tctggaaagt
    23461 tctccggcag aatttggtga tatttcttcc ggatatattg agatatcttt ttctaagcca
    23521 atggatagga atacattagc caattctatt tacatctatc ctcccgtgca gaataaaaaa
    23581 attaccttgg atggagccaa tcttaaaatc catattaagg aacccttaaa gcccgatacc
    23641 aattacttta ttactttaac caagcgtctt aaggatttac gtggcaacac cttggaaaag
    23701 aatcaaaccc ttatttacag caatggaaaa cttgccacca atcgtattgc tggaacaatc
    23761 acttatgaag aaccaactga taagggtttg cctttggagc taactctttt gtctgccgat
    23821 tctttattgg ttttaagcag tgttattcaa ggtggtgctt atgccattga caacttgaat
    23881 cctcagaaac atatcctccg tacttatata gataaagatt ttaatggtcg ttacgatttt
    23941 ggtagagagc cgttttttga agggattacc gatggttctt ccgtggcaac tttaaatata
    24001 acaatggctt acggtgatac tacgagagct aaaatatccc aagtgaatgt gcttagtgac
    24061 cgcgaactgc aagttatttt aagtgaagat attaagagct atgatgcact taacatccag
    24121 acaaaaaata cccctttatc cattgcctat caattgattg aaggatatat aattaattta
    24181 ttaaccgcca aaatggatag cacggaatat accttaaccc ttactggagt gaaagataaa
    24241 aaagataaca tcagtaacaa actggaaacc aaatttaaag ttagacccgt agctgatact
    24301 ttggctccca aaatcatttt tacaaatccc agaaacggtg cttcggtaaa tactctttta
    24361 cctgttttgg aaatccattt taatgaaatt attccgaaag ccaatattaa agccaaattg
    24421 ctctgcggaa atcaggaaat acctctcaaa catttgtctg aaactggacg cattcaccgt
    24481 tttcaacccc caaaagaact ggaaaattac aaaagccacc tgctaattat cagttcagaa
    24541 accacagatt ttagtggcaa tcaccttaaa aatgattatg aattgcagtt tctgcctctc
    24601 ctgagaaaat agatatcggg ataatagctc tggaaggaaa aggatttaag ggatttttaa
    24661 tttaaggttg agaggattgg agaatatttt aactcacaat tttctcccgc tttccagaaa
    24721 aacacaatct acgaactatc ttatttacaa ttccatttta actggtttcc aaattgaaaa
    24781 cttgtgagtt aaaatagaat actcctaact cctacaaaat aaatataatt ccgttttctt
    24841 ccttaatagc attgcttacc cattgcttac ccattactaa tggcattagc aatgagtccg
    24901 tattgagtaa gcaatgctac ttgggacaat aacttacaaa agtgtaaacg aaggatgccg
    24961 ttcctccgca ggaataatgg ttaaaaaaaa gtatttgtag ataactccgt aatgggcaaa
    25021 aaaatatttt gttacagcag aattagaagc ggataaaggt aataaaagaa aatgccttca
    25081 ctctcatcag atgagtaaag gcattaaaca atttaatatc ttaaaaaaag agcttaccat
    25141 cttaaatggg caaaagcttt attggcttca gccattttat gggtatcctc tcttttcttt
    25201 atggaagcgc cttctttttt atgagctgca ataagttcgg cggcaagttt ttcggtcatt
    25261 gtcttttcgc ttcttgcgcg tgcgttggca ataatccagc ggaaagctaa tgctttaccg
    25321 tttttttcat ttacctcaat aggaatttga taggttgctc ctcccacgcg tcgggaaaca
    25381 actttaacta aaggacgaac attatccaag gcggatttga agacaacaag ggggtcttct
    25441 ttcgttttag cagcgattat atcaaaagca ccgtacacaa ttttttcggc gatgcttttt
    25501 ttgcctttaa ccatcaggca gttcataaat ttggtaacgg taatatcgtt gtatttagga
    25561 tccggaagga cttcccggac aacagctcta tgttttctgg gcataagttc tatcctccct
    25621 ttactttacg gtttttgctt taggtcgttt ggttccatat ttggaacggg acttttcgcg
    25681 tttttctact ccagcggaat caagagctcc gcgaacgata tgataacgaa cacccggaag
    25741 gtctttcacg cgtcctccgc gcactaaaac aatagagtgt tcctgtaagt tatgaccttc
    25801 accgggaata tatgctgtaa cttcaaatcc atttacaaga cggacacgag ctacttttct
    25861 gagagctgaa ttaggttttt tgggcgttgt tgtataaaca cgagtacaaa ctccgcgcct
    25921 ttgcggacat tccataagcg ccctgttttt tcttttcttt tcaacttcag ctcttccttt
    25981 acggactaat tggttaatag ttggcactga ttttctcctt ttctatattt ttagtgttta
    26041 agattatttt tagaaatcaa ggtagtcccc gctttcttct tcttttgaac gatgtgcaaa
    26101 ctcatcaata atctgagcaa cagttttacc ttcgttaact gcctttttaa gcatattgtt
    26161 atagagtttg gttccagttc cgacaggtat tctgtgaccg ataataatgc tttctttcaa
    26221 gccctcaaga tgatcaaccc tgccctcaat agctgccttg gtaagcactt ttgtggtttc
    26281 ctggaaggaa gctgcagaaa gccagctatc ggttaacaga gatgttttgg taattcctaa
    26341 aagcaattgt tcaaactggg caggtgtttt gccaaattca attgcttctc ggttttcttt
    26401 ttccaccaga actttatcaa caatatcacc ctgcaggaaa ctggtgctgc cgctatctgt
    26461 aattctaact tttttaaaca tttggcgaat aataacgctc acatgtttgt cgtcaatttt
    26521 cacaccctgc ttgcggtaaa tttcctgaat ttcgtttaaa atcaggattt gagcttcaat
    26581 aacccctttc actaacatat catgtgggtc aagaggaccg tcagaaagtg catctccact
    26641 ttctacatag tcaccctgat gaactataat ccgctttccg gaaggaatta tatactttct
    26701 gcctgcaaga ggttgatgca tagtaacatt atcacctgtt tccacaatac tttcctgctc
    26761 tggaggaacg gcgtaaagtt tgccgcaaag aggagttcct tttttaactg attctccctc
    26821 tttaaccaga atttgcaagc ccttagggac aggatattga accgggtttt gattgcgttt
    26881 agcaacctga ataatcttct tccggttttc ttcaataata cttaccttgc catcgttttc
    26941 actataaaca gctttgtcgg gaaggacggc aatatattga tttttatctt catcataaag
    27001 gactacgacc tttccatcac aaggtgcgaa aagtccgtta ggtggggtaa caaaaatatc
    27061 tcttccggtt ttctttaagc caccgatagt aacaattccg tcaatatcgg aaatttttgc
    27121 tttatctttg ggaactcttg cttcaaaaag gtcttgcaca cgaggtaaac cacctgtaat
    27181 atcgcgttgt ttaattgtca ttcgtgatgt ctgacctaaa atatcaccaa tatggacaaa
    27241 gctgccatct tccacgcgga caataagtcc cgttggtaga ggtattaaac gcactgttcc
    27301 gtcttcacct acaattctga attgaggttg ttttctccgg tctttggatt caatgatggt
    27361 aagctcgcga gccatagtga tgtcgttata ttcttcttta taggtaatat ctttgatgaa
    27421 attctcataa tgcaaaacac ctttggctgt agagataaga ggattattga actgatccca
    27481 ggaaagaagt tttgtatctt tagctacctt ttgaccatcc tgaacatgaa cggtagctgc
    27541 gtattctact ttaaactctt ccaaaacctt attctcatca ttttcatcca caattaagat
    27601 tttacccaaa taactcacgg aaataagctg attttccgta ttgataacgg tattcatacg
    27661 atcaaatttt acaattccat catgcgaagc aacaacttct gccagttcgg ttgctgtaga
    27721 agcagcgcct ccaatatgga aagttctaag cgttaattgt gttcccggtt caccaatact
    27781 ttgagctgca ataattccaa caggatcacc aatagttgca ggtttctgag tagcaagatt
    27841 gcgaccataa catttggcac aaattccttt ttctgcttca caggtaagaa tgctgcgcac
    27901 atggacagag atcaaaccgt gattttgaat agttcttgcc attttgttac tgatttcttc
    27961 acctgcggaa actaatattc tacctgtgat aggacttacg atatcatcaa ctgctgttct
    28021 tccctgaatg cgttctgcca gggtttgcac aatttcattt ccttctttaa gggctgtcat
    28081 attcacaccc cttgtggttc cgcaatcttc aattgtgata atagcatttt gtgctacatc
    28141 aaccaaacgg cgtgtaagat atcctgcatc cgctgtttta agtgctgtat cagctaatcc
    28201 tttacgagct ccgtgagtgg aaacaaaata ttcaagaaca gtaagccctt ccttaaaatt
    28261 acttttaatt ggggtttcaa taacatctgc tccacccgtt gaaccaattt tagttggctt
    28321 atccattaaa ccgcgcattc cacccaattg tttaatctgg tctttgcttc cgcgggcacc
    28381 tgatttatac atcatataaa tagaatttaa gccgtttctg gttttagaaa gctcttccat
    28441 catttcatcg gtaacgcgaa cagttgtttt cttccattgg tcaacaactc gtccgaagcg
    28501 ttcattttcc gtaattccgc ctttctggtg aagatcggtt atttttttca cttcctgatc
    28561 ggcttgagca ataatttcat ctttccgttt aggaactaca atatcatcaa agctgaaagt
    28621 aacacctgca cgagtggcat aatgaaatcc caattcttta atctgatcca gaacctgagc
    28681 tgttctccat tgtcccactg catcaaaaca aagcattgcc aaatcattca atttcccctt
    28741 gtcaaatacg atgttttgaa agccgacttc cggaggcatt atctggttga gaataaccct
    28801 tcctacagtt gtaacaatat attcggattc caatttcaga cgaatccagg tatgcaggtc
    28861 taaattgcgt tcataaactg tttcaccttt ggagctgcaa aattgttctt ctgcttcgta
    28921 agcagcaata acttcatcag gaccgtaaaa atgtctcaat ttttctattt ccacaggtgg
    28981 cggtgtttct tccatagtta aataataaca gcccaaaacg atatcctgat tagccgccat
    29041 agccaaacgt ccatttgctg gtaacaataa atttcgggta gaaagcatta ggactcttgc
    29101 ttcaatttgt gattctacag acaggggaat ataaacaccc atttggtcac catcaaaatc
    29161 ggcattaaaa ggaacacaga ccataggatg aagctgaatt gctttgttat cagttaaaac
    29221 aggcataaat gcctgaattc ccaaacggtg aagagttgga gctctattca acaaaacagg
    29281 ataatcttta ataacttctt ccaaaattga ccatatttca ggttgctttt tcgcaataag
    29341 cttcttggca tttttaacct tatccacttc tccaattttc tgcagtttat caataagata
    29401 tggcttaaaa agctccacag ccatttcttt gggaagtccg cattgataaa gtttaagttc
    29461 aggacccact gtaatcacgc tccgtccgga ataatccact cgtttaccta aaaggttttg
    29521 acggaagcgt ccctgctttc ctttaagctg atcggttaat gactttaagg gcctattacc
    29581 tcttcctctt acaggacgag cttttctacc attatcaatg agggcatcaa ctgcttcctg
    29641 caacattctt ttttcattac gaaggataac ttctggagca cgaatttcca gtaatgcctt
    29701 cagacggttg tttctggtga taactcttct gtataaatca ttaaaatctg cagtggcaaa
    29761 acgccctcct tccaaaggaa ctaaaggtct taaagttgga ggtaaaacag gtaatgcttc
    29821 caaaatcata tcttcgggac gattgccgct tttgatgaag gcatctacta ttttcaattt
    29881 attgattagc ttctgtttac gcaaagccga ttcttcaaat ttaagccggg ttctgaggtc
    29941 aagtgcttcg tttttcaggt tgatacgctt aagcaattcg cggattgctt ctcctcccat
    30001 aagagccagg aagttatccg ctaccttatc tttaatctca taatattcat caacttccag
    30061 aagctccatt ttctcaaaag gactatctcc ggggtcaatt acaatgaagg actcgtagta
    30121 aagcactctt tccaggtctt taaccgtcat atcaagtaaa gtgccaattt tactcggggc
    30181 acttttaaca aaccagatat gagcaatcgg aactgccaaa tctatgtgtc ccatacgcgt
    30241 tctgcgaacc ctggaagtag ttacaagcac tccacatcta tcacaaacag ttccctgaaa
    30301 tcttttcttt ttatattttc cgcaagcgca ttcataatcc ctttcaggac cgaaaattct
    30361 ttcacaaaat aaaccatctt tctcaggttt gagagtgcgg taatttaatg tatcgggttt
    30421 tgtaacttct ccatgcgacc aatcattcct gatagtttcg ggagaggcaa ttttgatgcg
    30481 gactgcatca tattcattag gtttgatttc gtgtttactt tcttttaaca ttttctctcc
    30541 ccttctactt gttctcttct tctttgataa attcaatatc aaaacccagc gatttcagct
    30601 cgctgataag aacattaaag gattcaggaa ctcctggagg aggtggattc tcaccacgag
    30661 tgatagcttt aaaagcattg ttgcgtccat ccacatcgtc acttttgata gttaacatct
    30721 cttccaaaac gttggcagca ccgtatgctt ccaaagccca aacttccatt tctcctaaac
    30781 gctgacctcc atgttgagct tttccaccta agggttgttg ggtaataaga gaatagggtc
    30841 ctgttgagcg cgcgtgcatt ttatctgcta caagatgatt cagtttcatc atatagataa
    30901 tgccaaccgt tactctttct ttaaagggtt cacccgattt accatcatag agaactgttt
    30961 tcccatcttc cggtaaacct gctttgcgta attcagaaac aatatcctcg ttagaagcgc
    31021 catcaaaaat aggggtttct acttcaaagc caagtgtctt ggcagcccaa cccaaatggg
    31081 tctccattat ttggccaata ttcattcggg aaggaactcc caaaggattt aacacaatgt
    31141 ccacaggggt tccatcttcc ataaaaggca tatcttcaat aggagcgatg atagaaataa
    31201 cacctttatt tccatgacga cctgccattt tatcgcctac ttcaatcttg cgttttttgg
    31261 caacatacac tttaaccatt ttccgcacgc catattgaag ttcatcacca tgcttaattc
    31321 tttcgcggga ttttttatag atattatcgc tttgttcaag tgattgtttt attttcagga
    31381 caatttggga atagatatag tcgtttactt ccctatcttc aaccagttcc acatccagat
    31441 caagcttttt gaagtttatc cgtttcaaat cctgtttcgt tatcttcttc ccgggtgcta
    31501 taaagggaat attggttttt tcatcccaaa tatttttagc aatttgtccc actaatactg
    31561 cagaaagttt ttcttcctta aattcttcaa tcttactgcg tcgcagggct aattcttctt
    31621 ttagtttttc tatcttatct tcattttctt cttccaaatc gctgccttct tccaaacggg
    31681 aaaagacctt tacatcaata actacaccct ccattccagg tttggctttt aaggaactat
    31741 tggtaaaatc accggcacgg tctccaaata gagcacgcat caggttttct tccggtgaag
    31801 gatcaatttc tacactcttg ggagtaactt tacctacaat tatatctcca gcatgaataa
    31861 cacttcctac tctgattatg cctgttttat ccaaatttct caatgcctga gcaggaacat
    31921 tgggaatatc ataagcaagc tcttcacgcc cgtttttcac attacgaacc aaaacttcca
    31981 attcttcaat ataaatagat gttaaagtat cttcactggc taccttttcg ttaagaataa
    32041 cagcatcttc ataattgtaa ccataccagg gcataaatgc aaccattaaa ttagttccta
    32101 atgccaaacg attatcttcc acacaagcac cgtcagaaat tggctgccct ttttttacgg
    32161 tatccccaac acgcacaata ggacgctgat tgatacaagt atcctgatta gtgcgcacaa
    32221 atttttttag atggatgcga ttaccggtag aaagataata cgcctcatcc ttttcattta
    32281 taggtttcag gtctataaag gcagaagtaa ctttcgtaac tataccatca taaggagcta
    32341 ctgcaatgga agaagtatcc attgaagcaa tcttttccat tcctgttcct actaaaggtg
    32401 cttgaggatt aattaaagga actgcttgtc tctgcatatt ggaacccatt aaagcacgat
    32461 tggcatcatc atgttccagg aaaggaatta atgctgctga tactgaaacc atctgttgag
    32521 gtgctacatc catataatga acaaggtttg ggctaacttg aacaaattca ccccggtcgc
    32581 gggcaaaaat catttcatct gtaattctac ggttgtcatc caaatggaca tcactttgag
    32641 caataatata tttctcttcc tgtgaagcat caagatattc atactcatca gttatgcatc
    32701 catcaacaac cttgcggtag ggtgtttcta aaaaaccgaa atgatttata cgggaatata
    32761 ttgccggaga tacaattaaa ccgatgtttg gtccttcagg tgtttcaatc ggacaaattc
    32821 taccatagtg agaagaatga acatcacgaa cttcaaaacc agctctttcg cgagccaaac
    32881 ctccaggtcc taaagcagat aaagctctct tatgtcttaa agctgccaaa ggatttgtct
    32941 gctccataaa ttgggataac tgacctgtta aaaagaatga ctgaactact gaaataagag
    33001 cattgctgtt taccagatcg tgcactgtaa cttcatcaat attggaaatt gccatccgtt
    33061 cctgaattat tctggcaacc atagccattc caatagaata ttgttcctgg agcaattcac
    33121 caacagttct aactctgcgg tttgccagat tatctatatc atcaacctca tcttcatcat
    33181 ggtagatatt gatcagggtc ttgaaaatat atacaaaatc ctcaacagtg agggtcatta
    33241 ttttaggatc aacattgata cctaaacggc tattgatttt gtagcggcct acttctccca
    33301 ggttataacg tttttcattg aaaaacattc tatccacaag ctgtttggcc gcttccagag
    33361 gggcatcttc tccgggacga attaagttgt aaattttctt tagagcttct tcctgattgg
    33421 ttgtagggtc ttttgccata gtgttttcca gcacttttct ggcaatttca taattgtaat
    33481 caattacttc aaccttagta atcttccttt ccgataaaaa cttaattaaa gaatccgtta
    33541 tctgctcgtt agccatagct ataggttcag gattgccttt ttcataaata tcggcgaaaa
    33601 gatgacgacc ttttgcctct tttaaattca atttttcgga tttatagaat gtcttcctca
    33661 aatcttcgtt agtggaaatt ccaattgccc ggaaaagagt tgttacagga agtttacgcc
    33721 gtttatctat atggacgaac attacatcat gaatatcaat atcaaaatcc agccaagaac
    33781 cgctgtaagg aattactttt gcagaataga gtgtttttcc actgggatgt ttctcctccg
    33841 aaaagaaaac accgggtgaa cgttgtagtt gtgaaataat tacccgttct gctccattaa
    33901 taacaaatgt cccctgttca gttatcagag gtatttctcc taggaaaact tcctgttcca
    33961 aagtgtcttt atgctcgcgg acaccttctc tgttttcaaa cacgcttaag cgcatttttg
    34021 cttttaacgg tgcctgataa gataaatttc gttcgcggca ttcttccaga gaatatttct
    34081 cctgcaacac attgtattct atgaattcca acaggaaatt accctttgaa tcttcaaggg
    34141 gaaatatgtt ttcaaatact gcctgcagac cttttttttc tctgcgttgt gggtgaatgt
    34201 ccttttgtaa aaaatcattg aaggaatcca cctgcatagc cagaagattg ggtatttcca
    34261 cttcagggat acccaattca gcaaatcttc cggaaattcg ggaataactt ttgacctttc
    34321 tcaaaagcgc tctccttttg gttcttataa accagaatgt tggctcttaa gtgttaattt
    34381 tttctttttc acgctattag gcgacattta gaatgataaa ctgactaatt ggctttttca
    34441 aaaccaaagt ttaaggatgg ttgtatccag aaattagtcc aaaaaaagtc cctgcggact
    34501 ttttttggac ctttatcctg at
//
LOCUS       DAOPFL010000008        30101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1487_length_30101_cov_67.638721, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000008 DAOPFL010000000
VERSION     DAOPFL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 30101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30101
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_30101_cov_67.638721"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLG69_01475"
     CDS             <1..182
                     /locus_tag="PLG69_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPI42210.1"
                     /translation="KHAKPTQQNPQGGIITMEAPIDASNVMLFNEKLNAVSKPVYQVR
                     EGHRIRVCKKSGDEL"
     gene            196..741
                     /gene="rplE"
                     /locus_tag="PLG69_01480"
     CDS             196..741
                     /gene="rplE"
                     /locus_tag="PLG69_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPI42211.1"
                     /translation="MNRLKERYKTQVMDNLMKRFGYKNPHQVPRLEKIVVSMGVGQAT
                     QNKAILDNAVKDMETICGRKPVVTKARKSISNFKLRKGMPIGCKVTLRNEVMYEFYDR
                     LISIAIPRIRDFRGIPADSFDGRGNFSFGLKEQTVFPEIEYDKIDMIRGLNITIVTTA
                     KTDEECRALLQELGMPFQKNE"
     gene            752..937
                     /locus_tag="PLG69_01485"
     CDS             752..937
                     /locus_tag="PLG69_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPI42212.1"
                     /translation="MAKKSLIIKQQRTPKYKVRKYNRCKICGRPRSYMRDFGMCRLCF
                     RKYASEGQIPGITRSSW"
     gene            956..1369
                     /gene="rpsH"
                     /locus_tag="PLG69_01490"
     CDS             956..1369
                     /gene="rpsH"
                     /locus_tag="PLG69_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPI42213.1"
                     /translation="MSISDPIADALTKIRNAYRAGHTQVIVNHNKLIEALVNILAEEN
                     FVNSFQVLDKDPEHKINYKRILIILRYTNAGEPVIQGIQRISKPGRRVYVKADKLPSV
                     YNNTGCAVISTSMGLMVDRDARLKNVGGEYICKVW"
     gene            1379..1951
                     /gene="rplF"
                     /locus_tag="PLG69_01495"
     CDS             1379..1951
                     /gene="rplF"
                     /locus_tag="PLG69_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPI42214.1"
                     /translation="MSRIGRAPIKLQPGTQVDVADNKITVKGKLGELNWSLLPGITVE
                     VEENLLKVKRADDSKSQRAFHGLSRALIQNMITGVTEGFQKILHIYGTGYSAEVVGPW
                     LKLTLGFSHDILLQIPEGLEVTTEAVPRSKGMRSDFQSIITIKGIDKQLVGEFAAEVR
                     GCRPPENYKGKGIRYFDEYVIIKAGKAGTK"
     gene            1967..2338
                     /gene="rplR"
                     /locus_tag="PLG69_01500"
     CDS             1967..2338
                     /gene="rplR"
                     /locus_tag="PLG69_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPI42215.1"
                     /translation="MITSSCKIKRDLRNRRHLSIRKRVSGTPERPRLVVFRSLKNIYG
                     QIIDDTQGVTLVSMSTIAKDIGSLAGKKKTEQSYEVGVKLGEKALAAGITKVTFDRAG
                     YKYHGRVKALAEGARKAGLEF"
     gene            2348..2866
                     /gene="rpsE"
                     /locus_tag="PLG69_01505"
     CDS             2348..2866
                     /gene="rpsE"
                     /locus_tag="PLG69_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPI42216.1"
                     /translation="MNYEQNHPDEEKLIEKIVETKRVAKVVKGGRNFSFTAIVVVGDK
                     QGNVGVGNGKANEIVDAIRKAKEKAVKNMFKVPIVKGTVPHEVVARYGASKVLIKPAA
                     PGTGIIAGGTARAIFEAAGIENILCKSLGSNTPTNVVKATINGLKSMRTLSDISRLRN
                     KTMAQLTGQEEK"
     gene            2863..3048
                     /gene="rpmD"
                     /locus_tag="PLG69_01510"
     CDS             2863..3048
                     /gene="rpmD"
                     /locus_tag="PLG69_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPI42217.1"
                     /translation="MKLKVTQIRSTINRIEDHKKVIKALGLGKIGKSRIHNDTPSLRG
                     MINKVAYLLKVEELKEE"
     gene            3051..3506
                     /gene="rplO"
                     /locus_tag="PLG69_01515"
     CDS             3051..3506
                     /gene="rplO"
                     /locus_tag="PLG69_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPI42218.1"
                     /translation="MLTLSDLERPSCKKNKKRLGKGQGSGWGHQAGRGHKGKKARAGG
                     NVPPRFEGGQMPLNRRVPKRGFKNIFRENYRVLNLYRLQDLDITELDIPTMEKLGLIP
                     GKGKKANAPVKVLAGVKDEFTKTVTIKANAFSQKAKEIIEKNGGKAEVV"
     gene            3529..4821
                     /gene="secY"
                     /locus_tag="PLG69_01520"
     CDS             3529..4821
                     /gene="secY"
                     /locus_tag="PLG69_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPI42219.1"
                     /translation="MFRIPDLKKKILFTALFLVLYRLGSFIPLPGVNATALKVFFEGA
                     REGGNTLFGLLDLFVGGSFERASVFALGIMPYITASIVIQLLGSIIPYFEKLRKEGAD
                     GQKKLNQITRYGTVALAAFNAITITMWLTNLSGGVVPRPNFLFHLTAIITLVTGTMIV
                     MWIGEQITEHGIGNGISLIIFAGIIARYPEGFVRMIQNIPGNPSYVWKAIISVVLMVL
                     VTAAVIFVTEAVRKIPVQYAKRIVGRRVYGGQSTYIPLRVNTAGVIPIIFAQSVLMFP
                     ATIAALLGGDSYFWTTLRNWLSPGAVLYTILYVGLIIFFAYFYTAIVLNPTEMAENMV
                     KYGGHIPGKKPGKKTAEYINSVLVRITLPGAIFFAFIALLPEIMSNRLDLPFYFGGTG
                     LLIVVGVALDTLQQIESHLVMRHYDGFMKKGKLRGRSS"
     gene            4823..5584
                     /gene="map"
                     /locus_tag="PLG69_01525"
     CDS             4823..5584
                     /gene="map"
                     /locus_tag="PLG69_01525"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPI42220.1"
                     /translation="MIYIKTPAEIEKMRKSCRIIGELLDALETIIRPGISTLEIDKFA
                     EDFIGSRGGKPSFKGYRVDGLPPFPGSICASVNEQIVHGIPNAKKILQDGDIIGIDVG
                     VVLDGYNGDAARTYKVGKISAEAEELLQVTKEALERGISAAIPGNRVGDISYAIGSFV
                     AFRGYYVADNLTGHGVGRNLHEEPQIPNTGKPGRGPRLSAGMTLAIEPMVNIGTNRVL
                     ENGWEFKVADNSLSAHFEHTILVTDNKPEILTVAD"
     gene            5598..5816
                     /gene="infA"
                     /locus_tag="PLG69_01530"
     CDS             5598..5816
                     /gene="infA"
                     /locus_tag="PLG69_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPI42221.1"
                     /translation="MSKAGVIEVEGVVTEALPNTTFRVTLENGHEILAHSSGKMRMNY
                     IRILPGDKVKVELSPYDLTRGRITYRYK"
     gene            5865..5981
                     /gene="rpmJ"
                     /locus_tag="PLG69_01535"
     CDS             5865..5981
                     /gene="rpmJ"
                     /locus_tag="PLG69_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPI42222.1"
                     /translation="MKVKASVKKICKDCKIIKRNGIIRVICSSNPKHKQRQG"
     gene            5993..6361
                     /gene="rpsM"
                     /locus_tag="PLG69_01540"
     CDS             5993..6361
                     /gene="rpsM"
                     /locus_tag="PLG69_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPI42223.1"
                     /translation="MAHIAGIELPKNKRLFIGLTYIYGIGRSSAREICKKANIDEMKK
                     VADLTMEEEKILRDIIHNEYVVEGALRTQVAMNIKRLMEIGCYRGLRHKRGLPVRGQR
                     THTNARTRKGPRGSTIKKKK"
     gene            6370..6759
                     /gene="rpsK"
                     /locus_tag="PLG69_01545"
     CDS             6370..6759
                     /gene="rpsK"
                     /locus_tag="PLG69_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPI42224.1"
                     /translation="MAAKTRTKKKRVRLAFDEGLFYVHSSFNNTIVTLTDRAGNVLAW
                     SSGGKVGNKGSRKSTPFAAQIAATEVAKAGLDMGIQKVGVIVKGPGGGRESAIRAVNA
                     AGMKVTMIKDATPIPHNGCRPPKTRRI"
     gene            6772..7389
                     /gene="rpsD"
                     /locus_tag="PLG69_01550"
     CDS             6772..7389
                     /gene="rpsD"
                     /locus_tag="PLG69_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPI42225.1"
                     /translation="MAKYTGPKAKICRKFGENIYGNAKYDKILSKRKYPAGQHGRNLR
                     RKLSDYGLHLKEKQKMRHTYCLMEKQFSNYFKKAAKAPGVTGDNLLQLLERRLDNVVY
                     RMGFAVTRMQARQFINHGHFLVNGRKVDIPSYLVKAGDIIEVRPKSKGMKPLLEAMDK
                     TEVTSPYSWLSVDKENFRGQFNSIPAVSEIPVTCDLRLIVEFYSK"
     gene            7415..8590
                     /locus_tag="PLG69_01555"
     CDS             7415..8590
                     /locus_tag="PLG69_01555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPI42226.1"
                     /translation="MMYLEPLQMPNEVDYDKASYSRTYGKFEIGPLEPGYGTTIGNTL
                     RRILLSSIQGAAVRFVRIEGLHHEFCPIPGTNSDYIDLILRLKQLVISSSSVEEVPLV
                     LEHKGKGVITASMIQETAYVKIINKDLYLLEVVDDIDFRLEMIVGIGRGYVSADRQNP
                     EGKAVGFIPVDSIYSPVLKVNFSVSHQRVKERMNFDRLIMEIYTNGAVEPSIALFLAA
                     KILKDMAAKISLFEIEPQYIRDIELDPELEEKERILKTSVKEIELSVRAANCLAQAKI
                     ETIGDLVSKTEAEMLKFRNFGKKSLEEIVQKLKKYDLHLGMDVEGIYRQIREARSRGV
                     IPAEEKEIPAPETKTKTKPKTKTVAEKPAPKEKKTAESVTTATVRKPVPSSKKKVEK"
     gene            8587..8994
                     /gene="rplQ"
                     /locus_tag="PLG69_01560"
     CDS             8587..8994
                     /gene="rplQ"
                     /locus_tag="PLG69_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPI42227.1"
                     /translation="MRHRVEGRKFGREKDARNLMMKNLVKSMIEHGQLKTTLPKAKEL
                     RGYVERVVTYGKKDTVHSRRLAYKVLGDRTLVKKLFNEIAPAFEGRNGGYTRVLKAGF
                     RKGDNAPMAIIQFVEESTVKPKKDAIKAKDLNK"
     gene            complement(9785..12268)
                     /gene="leuS"
                     /locus_tag="PLG69_01565"
     CDS             complement(9785..12268)
                     /gene="leuS"
                     /locus_tag="PLG69_01565"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPI42228.1"
                     /translation="MEYRFSEIEKKWQNIWRERGIFNAPDRSSMPKYYVLSMFPYPSG
                     VLHIGHISNYSIGDVISRLKMMEGYNVMQPIGYDAFGMPAENYAIEHNSHPRLTTEEN
                     IAAMRIQFDSMGFGFDWSREISTCRPEYYRWGQYLFKKLYEKGLVYRKKGFQNWCDKC
                     QTVLANEQVVDGHCWRCGNIVEQKELEQWYFKITEYAEELLDFSQVIDWPERVITMQK
                     NWIGRSEGAKIDFILEGTNAKIPIFTTRPDTIYGVTFMALPPEHPLVQQWLREEPDNK
                     ELQDFCHKVINEDKVVRSSEETIKEGIFSGRYAINPFNGERIQIWITNYVLMDYGTGA
                     VMAVPAHDQRDFEFAKKYNIPIKIVIQKPDNSLKIEEMQEAYIEPGIMTNSDKFNGMD
                     SEQAKMAITDWARENGWGEKTVSYRLRDWCISRQRYWGNPIPVIHCPHCGIVLVPDED
                     LPVKLPDNVQVGRTTKNPLLSVPEWINVPCPKCGTPAKRETDTMDTFVDSSWYYARYT
                     DPNNDKEPFSKELADYWLPVDQYIGGIEHACMHLLYARFFHKFMRDLGWVNCDEPFAR
                     LLTQGMVTKDGAKMSKSKGNVVDPQYIIDRFGADTLRTFLLFASPPEKDVEWSDDGIM
                     GAFRFLNRVWRLVESNRETIKRGLQTGESSEPLSEEIYNLRYETHYAIFRWREDCLER
                     LQFNTAIASCMEFLNAIAKIKEPDKLNSAELKIYAFACVTLPKMLYPFAPHIAEELWQ
                     ILGNEKMLNECGLPEYEEKYLTRNNVTYVVQVNGKIRGKLEVPIDINSEDLKTKALEI
                     ENVQRFLQGLNIKKIIVVPGKMVSIAAGK"
     gene            complement(12460..12621)
                     /locus_tag="PLG69_01570"
     CDS             complement(12460..12621)
                     /locus_tag="PLG69_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42229.1"
                     /translation="MAYIYCLKTNFYFRDSPDTPNLVNPFYPVHPIPILIFNLRHLRN
                     LREPFFILF"
     gene            complement(13035..13706)
                     /locus_tag="PLG69_01575"
     CDS             complement(13035..13706)
                     /locus_tag="PLG69_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPI42230.1"
                     /translation="MLRLKRFKWSVLVIILQFIVMGYFLSILPADAKIPIHWNIHNEI
                     DGWTTKTNAALFNVAVSLGLFLLMFLMPYYSPWYRRYSKRNENFIPAITFILLLFLAL
                     INIYCLYIAARGKEPAIKLIQVLIGLLFIFLGNLLPKAPRNFFVGIRTPWTLANEEVW
                     IKTHRLGGCMFVFSGLIMVLNGFILTANKTLQEISGIFALVILLYPVLYSFLIFRKLE
                     NRNPK"
     gene            complement(13707..13979)
                     /locus_tag="PLG69_01580"
     CDS             complement(13707..13979)
                     /locus_tag="PLG69_01580"
                     /inference="COORDINATES: protein motif:HMM:NF033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HPI42231.1"
                     /translation="MFDSFFKAIADANRRKILLLLKKKGTMSAGEIATHFDISKPALS
                     EHLKILKNADLITAIKKKQYIYYALNTTVFEDILAFISEIINKSEV"
     gene            15107..15568
                     /locus_tag="PLG69_01585"
     CDS             15107..15568
                     /locus_tag="PLG69_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HPI42232.1"
                     /translation="MRVLANILWHFPFCGFMTALTAYIVGGLFFISVIGAPIGLGLFQ
                     FGKFLLAPFSYSMVSKKDLNIEQNKAWKTFGIIATIIYIPFGLALCVLTIFQIAGLCI
                     TIIGIPVAVVLAKSLGTYFNPVNKKCVPVSVAKEIDNRKSQKQVNDYLANN"
     gene            16727..18052
                     /locus_tag="PLG69_01590"
     CDS             16727..18052
                     /locus_tag="PLG69_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42233.1"
                     /translation="MLISFSFSNWKCFRDEVTFSLVAGREKHFTDRLPRVSKYKLKIL
                     PIAAIYGANASGKSALVSALAFAQNFIVGGTAPDQKIPLKPFLLDKEHKNMNSSFSFT
                     ILVDELIYEYSFTLSREKVIYEKLDSILKNSVKNIFERSGEGKKYKVQINNVAKEYRE
                     ALKFIGKHTIRKNQLFLTGTIYQNSDCFRPIYNWFKNSIVIIEPESQFIPILSLASEK
                     NKDLYSKLLNALDTGISDLTLQKRDVSKIEQKMLNTIFRDRIKNDEEYFMPDYGSKDR
                     TVYRISRDHIEANEIFSVHKDVDGEPVDFSFADESDGTNCLLDLLPAFIELKNQTGKV
                     YIIDELDRSLHTKLSKSLLKYYLNSCNENSRSQLIFTTHDVMLMDQNTFRRDEFWVTD
                     RSSEGVSSLYSFSDYKDIRSDKDLRKYYLQGNLKGIPRILISELYSDID"
     gene            18063..18677
                     /locus_tag="PLG69_01595"
     CDS             18063..18677
                     /locus_tag="PLG69_01595"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HPI42234.1"
                     /translation="MAGNGISKLKRKEKFISPSKLYVIVTEGETEVNYFEMIRKQIDS
                     NIYVVCKKGKHPDTDYLIKTANKYLKDHNCNELTHCWIVLDCDVLSDHNVESLKKWRK
                     TNNYSAKQEVGITSPNFEYWLILHFECPKKELTKQQCLTELKKHFPDYRKPFKNCNFN
                     IDQIQNAVKNGSMKEISLGERKNGTNLHLLLQDLILPSLELIMK"
     gene            19048..20013
                     /locus_tag="PLG69_01600"
     CDS             19048..20013
                     /locus_tag="PLG69_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HPI42235.1"
                     /translation="MEPVIISASRATDIPAFYSDWLISRVKAGYLRWKNPFNGQFQDV
                     SFAKTRLFVFWSKNPSPMLKKLSFFDEKGYNYYFQFTLNDYEQENWEKNLPSLQQRLN
                     TFIELSELIGKQKVIWRFDPFIISAKLHPDELLKRIENIGDQLFPYTNRLVISFIDIE
                     IYQKVKRNLNALPDNIQEIDTNTMHYFARELMRLNQKWNLKIATCAEKIDLQQYGIEH
                     NRCIDDRLIIELFSADTELMNFLGYRPTDQTDFLSDQNEKSSYSYEKLKDKGQRKFCG
                     CIKSKDIGQYNTCPNGCVYCYANSNPEMAWKNWNKAQRKQSDTIV"
     gene            19937..24166
                     /locus_tag="PLG69_01605"
     CDS             19937..24166
                     /locus_tag="PLG69_01605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPI42236.1"
                     /translation="MPIPILKWLGKTGTKHKGNKVTLSYDINNDPLFLALDKIETVTF
                     KDIVEKAKQYQVKGVKTDRGKKILETPEECNQYIVGYYVKHKDKMCDSLAKLFDYKGL
                     YNYDLNNIIIYDWACGQGLGSWLFLDEIKKRHLKITVSEIMLIDPSDIAIQRAKKFIG
                     YKDKSIKLTPIIKEFKELSTEEIRRDADTLRIHIFSNILDIDAIDLNNLSQLIIESQN
                     NQDYVIIAISPLKEDRLKNFIDTFPDKSKEKISVRICTHQTNCNDCSEENKGLCSISI
                     QNRNTKRIEYIYLIKTKHQTIKPVYIIPEKEEWVEEVFHKAQAAYFSDPVKKDMELMQ
                     QIKDKSYFQYEIVSGTSQPDEIQLSVLNVILNQIVRGIPTYLPIDIENLLVNQFSDLI
                     RRESKYESIQDSFIKPDIFFDPLLQAVFIDAPIYMAQTQYAILQHIKANFHPDIVKFT
                     LLETDKFYAHFSMLNLQNIFHNFMSLIPDLELPQIDYTVYCDFSNATLVDCNNKFGFA
                     IKDITTINEQDLSDEVIVYWDELPEMIKNKNHRKFINIKTLTTDYYDLEKFQSSGLIN
                     YGEFFEDEHKIKQDVFHSLQYFLQTIFRKEDFWPKQLEILQNTLSLKSIMAVQTTGAG
                     KSLLYQFSVLMQPGYCVVIEPIRSLMKDQADELEQLYIDAIYINSDLDATEKERRLYE
                     FTGGKSLFLLISPERMQMKDVKLQLACMDIYDRYHSYFVIDEAHCVSEWGHDFRPSYL
                     SLAKNAREYLKPKNNITLPIIALTATASLDVQHDVQKEMTSSEDNKPEIIADEIIREE
                     IDYFIFKEQDDGNKRKDKLHSLLESLTSESQSKPLSKDNAALIFCSYKAGKNGVKGNN
                     QDGYYYQLKDKYSNIGVGYFCGTDGVTEEEVNQMTKYQSDFKQNKIGIMVATQAFGMG
                     FNKPNIRYCFHIDLPKSVETFVQESGRIGRDKDKSNSYLIYTEGDYKFKKKLLTDDYL
                     NKEIAEEIFNSILDLRTDYNELEISYLLPKIKYNKGTYKFEQGIYFNKRIDSNMEGSC
                     FYNLSSHKLIQVEKKPMDEYTYNQIDEIIKRTNFLELMQNKRQYSLKDIIESNSNIIY
                     HLKLPHQLLIKELRGNSKIQAYLTNGRYKNNEKPYEEYKILISKCEYVLYRMQLLGII
                     VTYSRNYTDNIFEFDFRTPQLKEILEHYKDYLRKYLSEKELKQRIGQISEQLKDKSLM
                     AGYLVIFNDLSAYFDDTVYKKRKQSLNEMYNIAENFQGRSSEIIRKHFDLYFNSKFME
                     ELSSRTDSGKIEDIGIIAEFLQEVDNLSRGSRIENLNHLQGACARLLEESPDNYVLCI
                     LRSYAQLCSAEGNEKELESGVENLEKGWYKMLLDYENLDKMLLHNEVYLPYLTGLKQD
                     LSDTDYEKVFKKLSVLYVKAMLQDFNKYLTS"
     gene            24177..24857
                     /locus_tag="PLG69_01610"
     CDS             24177..24857
                     /locus_tag="PLG69_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42237.1"
                     /translation="MEQYENIMNELNSIIAEYKDNLVSLRAKFEDIKAIIDNLERMKN
                     QASKTTEEISELEKKYGVLAELYNKTDANLNETIADLSTQYQQLTEKQEQIVGIMESF
                     RKTQAEYAAQHNSAIHDLTIQEKADMENLHNYMEEVSGKINDEVIRALSQKMDVSTQF
                     LNRNLNIITKNQNKANSELEKNMMDRIQKCEKKLGSKMINCSIVIIILLAVIFFLIII
                     YTNQYSVY"
     gene            complement(25218..26294)
                     /locus_tag="PLG69_01615"
     CDS             complement(25218..26294)
                     /locus_tag="PLG69_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42238.1"
                     /translation="MAKVLYIEDEIAKNISTIIKFFRPILNDKRITNKLKDLENSDLL
                     NPQDIIDACRYNSSLDIAYNFPDALEKIIKNHQNYDLFIIDRNLSLFPYDKDLERIFN
                     LLEKYDYKSIDEIKEYYEREGDLLLCLLLKLNSDYQKIIYFLTANTNDGLRVLDDMKS
                     LIRSGNFNEEHIIEKGSENEEKIGIILSDLKSFKIQNQYPKQCEILRKHLDEDIVKQY
                     INMIKDKEANKDKWKDYLSKLRNILENILLDIAYNLKEPNAEYWNKKYKNELIINKFI
                     SNDGLSNYNKRNNIGYNSIIKNACYSIYGISSEFGIHNISKEVDIENINTYNLTNNTV
                     NSLLYQFNDVVLWYDKVLDIIQKQ"
     gene            complement(26287..28707)
                     /locus_tag="PLG69_01620"
     CDS             complement(26287..28707)
                     /locus_tag="PLG69_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42239.1"
                     /translation="MNKSNNQKTNPPIKNSEYEKLHAKFMNYMENKQFKEILDWMKEA
                     LKENEYDFYYRWRALAISKNEKSLLEDYYQALEDANRALELNYCENNLILKWQILSVL
                     DGAHADFGIRLYCYYCLDNNSEEICIEEPLDNNTIVKHKLSEEPKPLIPEDKKKYITE
                     EKFKTLYELIQLTDKLVYYKEMVKSIIYKAEANLNNLLLEKLQQVYQDISHHIDYENN
                     AEWYNLKREIEVKLEKWDEALLSTEKAYQDDNNLESYLRHKYEIYLQIKDYHKAIDVL
                     EQMIKNFPPPVVNPSDIFETNIPDSSYAHKLELLFITEQYDKYTKEVFNLYKQGLRIC
                     LSYYLQKLIQIKQYNAILNNLDEVLLANQVIGWFALCTYIEINNNAKASKIIDNINFD
                     TLLEEIANLYSFSLLKRFSYVQIYDDSYINLLFSSLNWQKMSDLLYIRFLAKLFEKGI
                     CDWSPQHNIITKYLEIKSPNYKFKEVAEELDTFSNNPDDLKVKLELLRYTYQEIDKDN
                     KVQSYLPFIRGLDNQLCNLIFKENEKKIMQAKLDERNRILSNLSHSIKNLLKAVIDPL
                     INLREDVPEKGVVIDNAIKGANLIREIVNAINYSYTTSLEDLRWDISHPDKERMSLQD
                     MIYDSLKYSISNMFDFRYFSPFALNYFASTLSNEEFKNIQNNWKTVSENNSKEALVKF
                     AENYLFLIDIFIDESGAYLIGNSKSSAIKMMILFQEIIFNAVKYVSYIPRENRIISIT
                     LKSDIDKLIFSVKNRFNPNITVKTTGIGNLLIDNFARALNCISEVKTDKQYYTVSLEF
                     MNLWRNNG"
     gene            complement(29048..29179)
                     /locus_tag="PLG69_01625"
     CDS             complement(29048..29179)
                     /locus_tag="PLG69_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42240.1"
                     /translation="MKRVVCGYFLLSCKLFRGLDKLETLSLRTNGMNQTKPEKGYTI"
BASE COUNT        10184 a         4980 c         5417 g         9520 t
ORIGIN      
        1 aaaagcatgc caaacccaca cagcaaaatc ctcaaggtgg tattataact atggaagcac
       61 ctattgacgc ttccaatgta atgcttttca acgaaaaact gaatgctgtt tccaaaccgg
      121 tttatcaagt tcgcgaagga catcgtatcc gagtttgcaa aaagagcggt gacgagcttt
      181 agaggagaaa aataaatgaa ccgtttgaaa gaaagatata aaacccaggt tatggataac
      241 ttaatgaaaa ggttcggtta caaaaatccg catcaggttc cccgattaga aaagattgta
      301 gttagtatgg gagtgggaca ggcaactcaa aataaagcta ttttagataa tgccgttaag
      361 gatatggaaa caatttgcgg tagaaaaccg gtagttacaa aagctcgaaa atctatctcc
      421 aacttcaaac tacgcaaagg aatgcctatt ggttgcaaag ttactttgag aaatgaagtg
      481 atgtatgaat tttatgatcg cttaatctct atagcaatac cacgaattcg tgattttcgc
      541 ggaattccgg ctgattcctt tgatggtaga ggaaattttt cttttggttt aaaagaacag
      601 acagtatttc cggaaattga atatgacaag atagatatga ttcggggatt aaatataacc
      661 atagttacca cagctaaaac cgatgaagaa tgccgtgctc ttttgcaaga gctgggtatg
      721 cccttccaga aaaacgaata aggagatata gatggcaaaa aagtcactca tcatcaaaca
      781 gcaaagaacc cctaaatata aagtaagaaa atacaatcgc tgtaaaattt gcggacgccc
      841 acgttcctat atgcgcgatt ttggaatgtg ccgtttgtgt ttccgtaaat atgcatctga
      901 gggccagata cctggaatca ccagaagtag ctggtaaaat aataggagga aaataatgag
      961 tattagtgat ccgatagctg atgcattgac taaaattcgc aatgcatatc gtgccggaca
     1021 tacacaggtg attgttaacc ataataaact gattgaagca ttggttaaca tccttgcgga
     1081 agagaatttt gtaaatagtt tccaggtttt ggataaagac ccggagcata aaataaacta
     1141 taaacgtatt ttaattattc ttcgttatac gaatgccgga gaacctgtaa tacaaggaat
     1201 tcagagaatt tccaaacccg gaagaagggt ttatgtaaaa gccgataaac tgcctagcgt
     1261 ttataataat actggttgtg cagttatttc taccagtatg ggtttaatgg tagatcgtga
     1321 tgctcgcctg aaaaatgtag gcggcgaata tatttgtaag gtttggtaag ggggaaaaat
     1381 gtcacgaatt ggaagagccc cgatcaaatt acaacctgga acgcaagtgg atgtagcgga
     1441 taacaaaatt accgttaagg gaaaattggg agaattgaat tggtctttgc ttcccggaat
     1501 tactgttgaa gtagaagaaa accttctgaa agtgaaaaga gcagacgata gtaaaagcca
     1561 acgtgctttt catggtctta gtcgtgcttt aatacaaaat atgattaccg gtgttacaga
     1621 aggatttcaa aagatattgc atatctatgg aacaggttat tctgctgaag ttgttggacc
     1681 atggctaaag ctaactcttg gtttttccca tgatattttg ttacaaattc ctgaaggatt
     1741 ggaagttact acagaagctg ttccgcgttc taaaggaatg cgttcggatt ttcaaagtat
     1801 aattactatc aaaggtattg ataaacagtt ggtaggcgaa tttgctgccg aagttcgcgg
     1861 ttgccgtcct cccgaaaatt ataaagggaa aggcataagg tattttgatg agtatgttat
     1921 aattaaagct ggaaaagccg gaaccaaata agggagaggg gaaaagatga taacttcaag
     1981 ctgtaaaatt aaaagagacc tccgaaaccg tcgtcattta tctattcgga aacgcgtaag
     2041 cggaactcct gaacgtcctc gtttagtagt ttttcgttcc ctgaagaata tatatggtca
     2101 aattatagac gatacacagg gtgtaacttt agtttcaatg tctactattg ccaaagatat
     2161 aggttcctta gccggaaaga agaaaacgga acagagctat gaagtaggtg taaaacttgg
     2221 tgaaaaagca cttgctgcag gaattacaaa agttactttt gatcgtgccg gatacaaata
     2281 tcacggtaga gtgaaagctc ttgctgaagg tgccagaaaa gctggtttgg aattttaagg
     2341 agcaaaaatg aattacgaac aaaatcatcc agatgaagaa aaattgatag aaaagatcgt
     2401 tgaaacaaag cgtgttgcca aagttgttaa aggaggcagg aacttcagtt ttaccgcaat
     2461 agttgtagtt ggtgacaaac aaggaaatgt tggtgtggga aatggcaaag caaacgaaat
     2521 tgtggatgct atccgaaaag ccaaagaaaa agccgtaaaa aatatgttta aagttccgat
     2581 tgtgaaagga actgttcctc atgaggttgt agctcgttat ggcgcttcta aagttttaat
     2641 aaaacctgca gctcctggca cgggaattat tgcaggagga actgccagag ccatttttga
     2701 ggcagcagga attgaaaaca ttttgtgcaa atccttgggc tctaacactc ctactaatgt
     2761 agtaaaagct actattaacg gtttgaaatc aatgcgtact ttatcagata tttcacgctt
     2821 gagaaataaa acaatggctc aacttaccgg tcaggaggag aaatgaaatt gaaagttact
     2881 caaatccgca gtactataaa tcgtattgaa gaccataaaa aggttatcaa agccctcggt
     2941 ctggggaaaa taggtaagag ccgtattcat aatgatactc catcccttag aggtatgatc
     3001 aataaggttg cttacctctt aaaagttgaa gaactgaagg aagaatagcg atgttaactt
     3061 tatccgattt agaaagacct agttgcaaga aaaacaaaaa acgcttggga aaaggacaag
     3121 gttccggatg gggacatcaa gccggacgtg gtcataaagg taaaaaagca cgtgccggtg
     3181 gaaatgtgcc tcctcgtttt gagggtggtc aaatgccttt aaacagaaga gttccaaagc
     3241 gtggtttcaa aaatatcttc cgtgaaaatt atcgcgtact gaatctttat cgtttacagg
     3301 acttggatat tactgaactt gatattccta cgatggaaaa gctgggttta attcccggta
     3361 aaggcaaaaa ggcaaatgca ccggttaaag ttctggctgg tgtaaaagat gaattcacta
     3421 aaaccgttac tatcaaagct aatgcttttt cacaaaaagc gaaagagatt attgaaaaga
     3481 acggtggtaa agcggaggta gtgtaacttg ttcaagacga ttggtaatat gttccggatt
     3541 ccggacctta agaagaagat cttatttaca gctctctttc tggttttata ccgtttggga
     3601 agtttcattc cgcttcccgg agtgaatgcc actgcgctaa aagttttttt tgaaggtgct
     3661 cgtgaaggtg gaaatactct ttttggtcta ttagaccttt ttgtgggtgg cagttttgaa
     3721 agagcatctg tctttgcctt aggtattatg ccttatatta cggcttccat tgttattcag
     3781 ctgttgggaa gtattattcc ctattttgaa aaattgcgta aagaaggagc tgatggccag
     3841 aagaaattga atcagattac ccgttatgga acagttgcat tggctgcttt taatgccata
     3901 actattacaa tgtggcttac caatctttcc ggtggagttg tgccgcgtcc aaatttcttg
     3961 tttcatttaa ctgccataat aactttggta acgggaacga tgattgttat gtggattggg
     4021 gaacaaatta ccgagcatgg aattggtaat ggaatttcac taatcatttt tgccggaatt
     4081 attgctcgtt atcctgaagg ttttgttagg atgattcaaa atattcccgg caatccttct
     4141 tatgtatgga aggcaattat ttctgttgtt ttaatggttt tagtaactgc tgccgttatc
     4201 ttcgttacgg aggcagtgcg taaaataccg gttcaatatg ccaagcggat agtgggaaga
     4261 agagtttatg gaggtcaaag tacctatatt cctttgcggg tgaatactgc tggagttatt
     4321 cctattatct ttgctcagag tgttttaatg tttccggcaa cgatagcggc tcttttagga
     4381 ggggatagtt atttttggac taccttgaga aattggttgt cgccaggagc tgttttatac
     4441 actattttat atgttgggct tattattttc tttgcctatt tttatactgc cattgtgctc
     4501 aatcctacag aaatggcaga aaatatggtt aaatacggtg gtcacattcc cgggaaaaaa
     4561 cccggtaaaa agaccgctga atacattaac agtgttcttg ttcggataac gcttccggga
     4621 gctattttct ttgcctttat tgccttgctg ccggaaataa tgagcaaccg tttagacctg
     4681 cctttttatt ttggaggaac aggtcttttg attgtggttg gtgtagcttt ggatactttg
     4741 caacagattg aatcccatct tgttatgcgt cactatgatg gttttatgaa gaaaggtaaa
     4801 ttaaggggac gctccagtta agatgatata catcaaaacg cctgcagaga ttgaaaaaat
     4861 gcgcaaaagt tgccgcatca ttggtgaatt attagatgct ctggaaacca taatccgtcc
     4921 tggaatcagt actttggaaa tagataaatt cgctgaggat tttattggca gtagaggtgg
     4981 caaaccttcc tttaaaggat atcgggtaga tggtttacct ccttttccag gttctatttg
     5041 tgcttctgta aacgagcaga ttgttcatgg cattcccaac gctaaaaaga ttttgcagga
     5101 tggtgatatt atcggtattg atgtgggagt tgttttggat ggttataatg gagatgcggc
     5161 aagaacttat aaagtcggca aaatatctgc tgaggcagaa gaacttttac aggtgactaa
     5221 agaagccctg gaaagaggaa tctctgctgc tattccggga aaccgtgtgg gcgatatttc
     5281 ttatgcgatt ggttcttttg ttgcctttag gggttattat gttgcagata atcttacggg
     5341 tcatggagta ggacgcaatt tgcatgagga accacaaatt ccgaatacag gaaaaccggg
     5401 aagaggtcct cgtttatctg caggtatgac tttagctatt gagccaatgg ttaatattgg
     5461 cacgaatcgt gtattagaaa atggttggga atttaaagtg gctgataata gtttatctgc
     5521 ccattttgag cataccattt tagttaccga taacaaacca gaaatattaa cagttgctga
     5581 ttgaggaata ttatactatg agtaaagctg gtgttataga agtggaaggt gttgtaaccg
     5641 aagcacttcc taatacaacc tttcgtgtta ctctggaaaa tgggcatgaa attttggctc
     5701 attcttccgg gaaaatgcga atgaactata tcagaattct gcctggtgat aaagtgaagg
     5761 ttgaactttc accttatgac ttaacgcgag gcagaatcac ctaccgctat aaataattat
     5821 aaatgattgc aaacagccat cttggctaaa gcaaggagat gataatgaaa gtgaaagctt
     5881 ccgttaaaaa aatctgtaag gattgcaaaa ttattaaacg caacggtatt atccgtgtta
     5941 tttgcagttc aaatccaaaa cataaacaga gacaaggtta aggaggaaaa acttggcaca
     6001 tatagcaggt attgaacttc ccaaaaacaa acgtctgttt atagggttaa cttatatcta
     6061 tggcattggc aggtcaagtg ccagagaaat ttgtaagaag gcgaatattg acgaaatgaa
     6121 aaaagtagcc gatcttacga tggaagaaga aaaaatccta cgcgacataa ttcataatga
     6181 atatgtggtt gaaggagcac ttcgcactca agtagctatg aatattaaac gcttgatgga
     6241 aattggttgt taccgcggtt tacgccataa acgcggactt cctgtccgag gacaaagaac
     6301 acatactaat gccagaacca gaaaaggtcc tcggggtagc acaattaaga aaaagaaata
     6361 ggagaaatta tggctgctaa aacaagaacc aagaaaaaaa gagtgcgtct tgcttttgat
     6421 gagggacttt tctatgtcca ttccagtttc aataatacta ttgttacttt aaccgatcgt
     6481 gcaggaaatg ttcttgcctg gtcaagtggt ggaaaagttg gtaacaaggg ctctcgtaaa
     6541 agtacacctt ttgcagctca aattgccgct acagaagttg ctaaagcagg tctggatatg
     6601 ggaattcaga aagttggggt gattgttaaa ggtcccggtg gaggtagaga atccgctatc
     6661 agggctgtaa atgctgcagg aatgaaagtt actatgatta aagatgcaac tcctattccg
     6721 cataatggct gccgtccacc caaaaccaga aggatataag gagagtaaca aatggctaaa
     6781 tatacaggac ccaaagcaaa aatatgccgt aaattcgggg aaaatattta tggcaacgcc
     6841 aaatatgata aaatacttag caagcgtaaa tatcctgccg gacagcatgg cagaaattta
     6901 aggcgtaagc tttctgatta tggtttgcat cttaaggaaa aacaaaaaat gcgccatact
     6961 tattgcttaa tggaaaagca attcagtaac tatttcaaaa aagctgctaa agcacctgga
     7021 gttacaggcg ataacttatt gcaattattg gaacgccgtt tggataatgt agtttatcgt
     7081 atgggttttg ccgttacaag aatgcaggcg cgtcagttta tcaatcacgg tcatttttta
     7141 gttaatggca ggaaagttga tattccttcc tatttagtta aggcaggaga tattattgaa
     7201 gttagaccca aaagtaaagg tatgaaacct cttttggaag caatggataa aacagaagtt
     7261 acttcgccat attcctggct gagtgttgat aaagaaaatt tccgcggtca atttaattca
     7321 attcctgcag taagcgagat tccggtcact tgtgacctgc gtcttattgt tgaattttac
     7381 tccaaataag gcgttataag cataaggagc agctatgatg tatcttgaac ctttacaaat
     7441 gccgaatgaa gttgattacg ataaagcatc gtattcacgc acttacggca agtttgaaat
     7501 cggtcctttg gaaccgggat atggcaccac aataggaaat accttgcgtc ggattttgct
     7561 ctcttccata caaggagcgg ctgttcggtt cgttagaatt gaaggtttgc atcatgaatt
     7621 ttgtcctatt ccgggaacaa attctgatta tatagattta attttgcggt taaaacaact
     7681 cgttatctct tccagttctg tggaagaagt tcctctggtt cttgaacata aaggaaaagg
     7741 cgtaataact gcctctatga ttcaggagac cgcttatgtg aaaattatca ataaagacct
     7801 ctatctgctg gaagttgttg atgatattga ttttcgtttg gaaatgattg taggtatagg
     7861 gcgcggttat gtttcagcag acaggcaaaa tcctgaaggt aaggcagttg gttttattcc
     7921 tgtagattcc atttattcac ctgttctgaa agtgaacttc agcgtttccc atcaacgggt
     7981 gaaagagaga atgaattttg accgtttgat tatggaaatt tatacgaacg gagccgtaga
     8041 gccatctatt gctctatttc ttgcagctaa aattttgaag gatatggcag ctaagatatc
     8101 tctgtttgaa attgaaccac aatatattcg tgatatagaa ttagaccccg agttggaaga
     8161 aaaagaacgc attctgaaaa caagtgtgaa ggaaattgaa cttagcgttc gcgctgccaa
     8221 ttgtctggct caagcaaaaa ttgaaaccat tggcgatttg gtttccaaaa cggaagccga
     8281 aatgctgaaa ttccgtaatt ttggcaaaaa atcactggaa gaaatagttc agaaactgaa
     8341 gaaatatgat ttacatctcg gtatggatgt tgaaggtatc tatcgtcaaa ttagagaagc
     8401 cagaagcaga ggagtaattc ctgctgaaga aaaagaaatt ccggctccag aaacaaagac
     8461 aaaaaccaaa ccgaaaacta aaacagttgc tgaaaaacca gcacctaaag aaaaaaagac
     8521 agctgaatct gtaactactg caacggtgcg taaacccgtt cccagttcta aaaagaaggt
     8581 ggaaaaatga gacacagagt tgaaggaaga aaatttggtc gggaaaaaga tgcccgcaat
     8641 ttgatgatga aaaatcttgt aaaatcaatg atagaacacg ggcaactgaa aacaaccctt
     8701 cctaaagcta aagaattaag aggatatgtt gaacgtgttg ttacttatgg caaaaaagat
     8761 acagtccatt ccagacgtct ggcttataaa gtattgggcg atagaacact ggtaaaaaaa
     8821 ctatttaacg aaattgctcc agcttttgaa ggtcgtaacg gtggctatac acgtgtgtta
     8881 aaagctggtt tccgcaaagg ggataatgca cctatggcta ttattcaatt tgtggaagaa
     8941 tctactgtga aacctaaaaa ggatgccatc aaagctaaag acctgaataa ataaaaaact
     9001 ctcaataact acatggattt ggattcacaa agagataaag agaattaatg aagaatgggg
     9061 aatggagaat tgagaatgga aaatggaaat ttgagaatgt aaggaagtca ggagtcgtca
     9121 tcgctgaaat aaattattac ctatcctcaa acttatttag cgatgaggac tcgtgacgac
     9181 tttgattata tagctggagg acgctgttcc tccgcaattt ttagccaccg aaaatcccca
     9241 aaaaatactc tcacagattt cagggatatt tttggttaat aagtcaggaa tgaacaagga
     9301 aaaagggatt ttagggttat ttttaattat attcaaaaat attatgcttt ctgtttatgc
     9361 caaaccggaa ttcggtatcc catttataaa tgtgccttat aactatttcc aaaccattgt
     9421 tggtgcttat tttctgcata attatttgtg gtagatatag ggtggcttat ctcggtaatt
     9481 tgccgagagg cgtcaagata ttactaccgt agataattaa gtatgatgct tttcctaatg
     9541 atcgaaattc tcgtcaaccc ctgtcaacca agcatagaat catattattt cttaaatgaa
     9601 aagacccttg tcatttctaa gcaagcaaga atctggataa gttccgatat aataattgca
     9661 ttttccagat gaaaactata ttattcccgc ttttttacat cggggacacc gagaatactg
     9721 aaaacaccga gatatttttg gggtctctca ttatattcat tttttatgct tcattcttaa
     9781 ttcactattt tcctgcggca atagaaacca tttttcccgg cactactatt atctttttaa
     9841 tgttcagtcc ctgcagaaag cgctgcacat tttcaatttc cagggctttg gtttttaagt
     9901 cctccgaatt aatgtcaatt ggaacttcca gtttccctct tatttttccg tttacctgaa
     9961 ccacataagt aacattattt cgggttaagt atttttcttc atattcagga agtccgcatt
    10021 cattcagcat tttttcattt cccaatatct gccaaagttc ttccgcaata tgaggagcaa
    10081 agggataaag cattttaggc aaagtgacgc aagcaaaagc gtaaatcttt aattccgcag
    10141 agttcaattt atctggttct ttaattttcg ctatggcatt gagaaattcc atacagcttg
    10201 caatggcagt attgaattgg agacgctcta aacaatcctc tctccagcga aatatggcat
    10261 aatgggtttc ataacgcaag ttatatattt cctctgaaag aggttcagaa ctttcacctg
    10321 tctgcaaacc gcgttttatg gtttcccgat tactttctac caaacgccaa acccggttca
    10381 aaaatctgaa ggcacccata attccatcat cgctccattc cacatctttt tcgggaggtg
    10441 aggcaaaaag caaaaaagtg cgtaaagtat ctgcaccaaa gcgatctata atatattgcg
    10501 gatcaacaac attgcctttg gatttgctca ttttggctcc atcctttgta accatcccct
    10561 gtgtaagtaa acgtgcaaag ggttcatcac aatttaccca acccaaatcc cgcataaatt
    10621 tgtggaaaaa acgggcataa agcaaatgca tacaggcatg ttcaattcca ccaatgtatt
    10681 gatctacggg aagccaataa tcagccagtt ctttgctaaa gggctcttta tcgttattcg
    10741 gatcggtata acgagcataa taccaactgc tgtctacaaa agtatccatt gtatccgttt
    10801 ctctttttgc tggagttccg catttcggac agggcacatt tatccattcc ggaacgctca
    10861 aaagcggatt tttggttgtt ctgcctactt ggacattatc cggcaattta acaggtaaat
    10921 cctcatccgg aactaaaact atcccacaat gcggacaatg aataaccgga atgggatttc
    10981 cccaataacg ctgacgcgaa atacaccaat cccttaaacg ataactaacg gttttttcac
    11041 cccaaccatt ttccctagcc caatcggtta ttgccatttt ggcttgttca ctatccattc
    11101 cgttgaattt atcggaatta gtcattattc cgggttctat atatgcttct tgcatctctt
    11161 caatttttaa ggagttatca ggtttttgaa taactatttt aatcgggata ttgtattttt
    11221 ttgcaaattc aaaatcgcgt tgatcgtgag ccggaacagc cataactgct cccgttccgt
    11281 aatccataag cacataattg gtaatccaga tttgaattct ctcgccatta aaaggattta
    11341 tagcataacg accactaaaa atgccttctt taattgtttc ctcagaactg cgcacaactt
    11401 tatcctcatt aataactttg tggcaaaaat cctgcagttc tttattatcc ggttcttccc
    11461 tcagccactg ttgaactaaa ggatgctccg gcggcaacgc cataaaagtt accccgtaaa
    11521 tagtatccgg acgggtggta aaaatcggta tttttgcgtt tgtcccttcc aaaataaaat
    11581 caattttagc accttccgaa cgtcctatcc aattcttttg catcgtgatc actcgttcag
    11641 gccaatcaat cacttgggaa aaatccaata gttcttctgc atattccgta atcttgaaat
    11701 accattgttc cagttctttc tgctctacaa tgtttccaca gcgccaacaa tgaccatcca
    11761 caacctgctc gttagccaaa acggtttgac atttatcaca ccagttctga aacccttttt
    11821 tccggtaaac caatcccttt tcatataatt tcttgaaaag atactgtccc caacgataat
    11881 actccggacg acaagtgctt atttctctgc tccaatcaaa accaaaaccc atactgtcaa
    11941 attgaatacg catcgctgct atgttttctt cagtagtaag gcgcggatgg gaattgtgct
    12001 caatagcata attttccgca ggcataccaa aagcatcata accaatgggc tgcatcacat
    12061 tatatccttc catcattttc aatcgcgaaa tcacatctcc aatagaataa ttggaaatat
    12121 gacctatatg caaaactccc gaaggataag gaaacattga caatacataa tatttgggca
    12181 tggaacttct atcaggtgca ttaaatatcc ctctctcacg ccaaatattc tgccatttct
    12241 tttcaatttc actaaatcgg tattccattc tatctctccc ggtgtatcat tttatcttat
    12301 aataccttgc aaaatttggg agaccgcata attgtcaagc aaaagctgta aaggaatttt
    12361 gacccacaat tttcacctac tttcacagaa accacacccc acaaacattt tcgttttcta
    12421 cccctctata actctttatc taattaaaaa aattgggagt taaaatagaa taaaaaaggg
    12481 ctcacgcaga tttcgcagat gacgcagatt aaaaatgaga ataggtatgg gatgaacagg
    12541 ataaaaggga tttacaagat ttggagtatc ggggctgtcc cgaaaataaa agttagtttt
    12601 gagacagtaa atataagcca ttttaaggaa caatctcctc tttaggatac atctttgtat
    12661 acctgaaatg gtaaaaaatg gacttttgaa aggttgtgag acaaccccga ctgctattat
    12721 agaagggttt caacccggcg aaaagaagac agaataaaaa aaagttccgt tggaactttt
    12781 tcatcttggt ccaatagtta ttatggggta aaatgtaatt tttatatcta actatatacg
    12841 ctttagcaaa ccacaaaagg gaggagcttt gaattatata aacattgatt taatatgcca
    12901 attattcaac ggtctttcaa ttactataac tgacttttcc atatagaaat tatataatat
    12961 cctgcctgtt aaccggaaag ggactaattc ttttcagctg ttttttcctg ttttgcgttg
    13021 ttgtttttaa ctctttactt tggatttctg ttttccagtt tgcgaaagat aagaaatgaa
    13081 taaaggaccg gatacagcaa aatcaccaag gcaaaaattc cgctaatttc ttgtaaggtc
    13141 ttattagccg tcaggataaa accgtttaaa accataatca atccgctaaa cacaaacata
    13201 caacccccca aacgatgtgt ttttatccac acttcttcat tggcaagtgt ccagggagtt
    13261 ctaattccca caaaaaaatt gcgcggcgct ttgggtaaca aattacccag aaaaatgaac
    13321 aacaaaccaa tcaatacctg aattaattta attgccggtt ctttaccccg tgccgcaata
    13381 tatagacaat agatatttat taaagccaaa aataaaagta aaatgaaagt tatggctggt
    13441 ataaaatttt cgtttctttt gctataacgc ctataccaag gtgaataata gggcatcaaa
    13501 aacatcaata gaaacagtcc taaacttaca gccacattaa ataaagcggc attagtttta
    13561 gttgtccaac cgtctatttc attgtgaatg ttccaatgaa tgggtatttt agcatccgct
    13621 ggtaaaatag ataaaaaata tcccataacg ataaattgga gtataataac cagaactgac
    13681 catttaaatc gcttcagtcg taacatttat acctcacttt tgtttatgat ttctgaaata
    13741 aatgccagta tatcttcaaa taccgtagtg ttcaaggcat aataaatata ctgttttttc
    13801 ttgatagctg taattaaatc agcatttttc aaaattttca gatgctcact taaagccggt
    13861 ttggaaatat caaaatgcgt agctatttcc cccgcactca ttgttccctt ttttttcaag
    13921 agcagcaaaa tctttctccg gttggcatca gctatagctt taaaaaagga atcgaacatc
    13981 ttgcaactcc tttgcctaaa agcaagcagg tattattatt taattaaata cctaattaaa
    14041 caagagccga aatatgtcca gtattttctc ttctttttta tgcaaactta actttttttc
    14101 agagttaact actacgcagg caagaggttt taatatatct taagaataaa tttgcaaaaa
    14161 gataaaaaaa tccttgacat tttaatagca agataatcct aaaccaaaat cttatagtag
    14221 gagttactat tatgaaaaag tccttaatta ctctcgttct ggcacttttt gcaagtgccg
    14281 tcttttccac cgtctgaaat gtgaataaca tttccggaat agcagcagat tttaacgact
    14341 taattgtcgc ttgtaactcg tctgaattat gcgattatgg gactgtcccg aaactaaaag
    14401 ttagttttga aacggtaaac ataagccatt ttaaagaaca atctactctt tacgatacat
    14461 ctttgtatac ctgaaaatgg caaaaaatgg acttttgaaa ggttgtgaga caaccccatt
    14521 acagtagcat tggaagcaag cattgtgcat ttacagcaag acggtgaata tcaacttatt
    14581 ttttatacaa ccaatgctcg cggacaaaaa agtttgaaaa ggttaaaaat ctctatatgt
    14641 ttaaacttac gaggggctta cgcgcccatc gctatcattg tatcgccctg cgggctttta
    14701 aatttacaag ctaaaattta tcagacagtc acttctcttt ttatatatgt attatcataa
    14761 tggaaaatac ataagataca atggaggatt ataggttggc aaaactatat tcatccataa
    14821 aatgaattct gtaataagtg cggaatgaca aatacaagca tagtaaataa taatttcatt
    14881 gtcattcctg tgcaggcagg aatccattca aaatcacaaa agtaatcgca tattccatag
    14941 aaaaacttta ttatcaatcc atccttctat cctctatttc ttagataatg ttaactattt
    15001 atccctgcaa taacagaagg ctaaaaaatt catattgtta tttttgtgct tgacaacaag
    15061 attaaaatca aaaatatgaa acaaattcaa aaaaaggaga aatgttatgc gagttttggc
    15121 aaacattctg tggcacttcc cattctgcgg ttttatgacc gccttaaccg cttatattgt
    15181 tggcggactt ttttttattt ctgtaattgg tgcacctatt ggcttaggtc ttttccaatt
    15241 tggcaaattt ctgttagctc ctttcagtta ttcaatggtc agcaaaaaag acctgaatat
    15301 tgagcagaac aaagcttgga agacctttgg aataattgct acaattattt atattccttt
    15361 tggattggca ctttgcgttt taacgatttt tcaaattgcc ggtttatgta ttacaattat
    15421 cggtattccg gtagcagtgg tattagcaaa aagtttggga acctatttta atcctgttaa
    15481 taagaaatgt gttcccgtat ctgtggctaa ggaaattgat aaccggaaaa gccaaaagca
    15541 agttaacgat tatttagcaa ataattagtt atttatcgga caatttaaaa ggaagaaatc
    15601 aggagtactc actgccaaaa actctttttc atatttatgc tgagttatgg cagtgaggac
    15661 tcgtgatgaa ttagtttttc taacatattt cactgatatt agtaggtttt aacttactat
    15721 tttcacctgc cttcaatgta accacttatc cgcatttatt ttcacttaca accgctctac
    15781 gactggtttc caaaccgaaa aatcgtgagt caaaacagaa agccctccgg aatatttacg
    15841 ctatttctct cccaaaattg ttgtttaaca atttctgctt ttttgcgcta tacggcggat
    15901 gagattccca tatgattccc atatcgcgat atgggaatga tatgggagtc atatcagaat
    15961 ttaattgaaa ttatttcggt ttttctttgg aagtgatatt cattaaattt tgctaatgtt
    16021 tgccgtatat tgagatagca gattacttga caattataga aagtgctgaa ccggttttct
    16081 atgaatttgt ggctgcattt tgttttgact cacaattttc aagtttgtag accagctaaa
    16141 aagcggttgt aagtgaaaaa gatcataggt gtaaggacat tgaaggcaga tgataattgt
    16201 gagttataac tgcctaatga caaccaaggt gtttcgttac cgcttgcctt tcggttaaga
    16261 aataaagcgt atgatgtggt tgacgaggat gtcaattttc cggtataaaa atagtttgaa
    16321 ccaacagcga atattttcct gatctgacag gtataaatta ataagttaaa gatgggtaag
    16381 aaatatatag taatatcctt tcccttttga aaattcattc gtcaatcctg tgaaagcaca
    16441 atacagaaat actaaaatac tattgctgac tttttcatat agaaataata tgatattatt
    16501 ttcatactgg aaagacataa atatcttgct aatcacacat aaattttttc tttctaagga
    16561 atggcaagtg gcttttattt aagaagtgaa ataactcatt aaaaaggaat ccttaaagga
    16621 tgagatatca aatataagag aaaatttctc ttgacaatat gatgatgatt taatatagag
    16681 aatttattat tagttattag agagaaaatc tcttaaagga gccaaaatgc ttatatcctt
    16741 ttccttttcc aactggaagt gtttcagaga cgaagtaact ttttctcttg ttgccggtag
    16801 agaaaaacac tttacggata gattacccag ggtaagtaag tataaactta aaatattacc
    16861 tattgctgca atttatggag caaatgcttc aggtaaatct gctctggtat ctgctcttgc
    16921 ttttgcccaa aattttattg tcggaggcac ggcaccggat caaaaaatac ctttaaaacc
    16981 ttttttactt gataaagagc ataaaaatat gaatagctcc tttagtttta ccattctggt
    17041 agatgaactt atctatgaat atagttttac tctttcccga gagaaagtta tttacgagaa
    17101 attggattca atcctaaaaa attcggttaa gaacatattt gaacgctcag gtgagggtaa
    17161 aaaatataaa gttcaaatta ataatgtagc taaagaatat agagaagcat tgaaatttat
    17221 tgggaaacat acaattcgta aaaatcaact gtttttaaca ggaacgattt atcagaatag
    17281 tgattgtttt agacctatat acaactggtt caaaaattca atagttataa tagaacctga
    17341 gtcccaattt attcctatat taagtttagc aagtgagaaa aacaaagatc tatattcaaa
    17401 acttttaaat gcattagata ccggaatatc tgaccttacg ctacaaaaaa gagatgtttc
    17461 gaaaattgaa caaaaaatgc tgaatactat ttttcgagat agaataaaaa atgatgaaga
    17521 atattttatg cctgactatg gtagtaaaga tagaactgtt tatcgtattt ccagagatca
    17581 tattgaggca aacgaaattt tttctgtaca taaagatgta gatggtgaac cggttgattt
    17641 ttcttttgcg gatgagtcgg atgggactaa ttgtttattg gatttgcttc ccgcttttat
    17701 tgaattaaaa aatcaaaccg gcaaggtgta tataattgac gaattggata gaagtttaca
    17761 tacaaaacta agtaagtcct tattaaaata ctatcttaat tcctgcaacg aaaacagtag
    17821 aagtcagctg atttttacaa ctcatgatgt gatgttaatg gatcaaaata ctttcagaag
    17881 agatgaattt tgggttacag acaggtcttc tgaaggggtt tcttctctat attcctttag
    17941 tgattacaaa gatatccggt ccgataaaga tcttcgcaag tattatttac agggtaatct
    18001 gaaaggaatt ccgagaatat taattagtga actatattcg gatattgatt gaggttaaca
    18061 ttatggcagg aaatggaata agcaaactaa agcgtaaaga aaagtttata agtccaagta
    18121 agttatatgt tattgttaca gagggcgaaa ctgaagttaa ttattttgaa atgataagaa
    18181 agcaaataga tagtaatata tatgtcgtat gtaaaaaggg aaagcatccg gataccgatt
    18241 atttaattaa gacagccaat aaatacttga aagatcataa ttgcaatgaa ctaactcatt
    18301 gttggattgt tttggattgc gatgtcttaa gcgatcacaa tgtagagtca ttgaaaaaat
    18361 ggagaaaaac aaataattat tcggcaaagc aggaagtggg cataacctct ccgaattttg
    18421 aatattggtt aatattacat tttgaatgtc ccaaaaagga attaaccaag caacaatgcc
    18481 taactgagct aaaaaaacat tttcctgatt acagaaagcc cttcaagaat tgtaacttta
    18541 acatagatca aattcaaaat gcagttaaaa atggttctat gaaagagatt tctttaggag
    18601 aaagaaaaaa tggaaccaat ctgcatcttc ttctccaaga cctgatattg ccttctttag
    18661 aactcataat gaaataaaaa atctctcata gataacacag gtatcaataa taggttgtaa
    18721 ttataaaaaa agcccgcagg gcgatacaat gatagcgatg gtggtgtaaa cccctcgtaa
    18781 aaatataagc ccctcttttt tctcttttct ctctttttaa ctttcctgtt tacaaaacat
    18841 attagtcacc tgaaggttga cttcctcgga aactaaaaaa actggaaagt tgctgtcctc
    18901 gttaactatc tctcacgctt tattccgtta atgaaatgat agttagaccc ctcaataaat
    18961 aatttccttg tctgaaatac aattatttct aaaatggaat caatatccaa agtgagataa
    19021 taatttggag gcaaaaaaga aaacaatatg gagccggtaa ttatttctgc ttcccgggct
    19081 acggatatcc ccgcttttta ttccgactgg ttaatttcgc gtgttaaagc gggttattta
    19141 agatggaaaa atccttttaa tggtcagttc caagatgtct cctttgctaa aacccgactt
    19201 tttgtgtttt ggagcaaaaa tccttcccca atgctaaaaa aactctcttt ctttgatgag
    19261 aagggctata attactattt tcaatttacc ctgaatgact atgaacagga aaattgggag
    19321 aaaaatctgc cttctttgca gcaacgccta aataccttta ttgaactttc tgaacttatt
    19381 ggcaaacaaa aagttatctg gcgttttgat ccttttatta tcagtgctaa attgcatccc
    19441 gatgaactct taaaacgcat agaaaatatt ggtgaccagc ttttccctta tacgaataga
    19501 ttagttatca gcttcataga tatagaaatt taccaaaaag tgaaacgcaa cctcaatgct
    19561 ttacccgata atattcagga aattgatact aacacaatgc attattttgc ccgggaactg
    19621 atgcgactaa accaaaaatg gaacttaaaa atagctacct gtgccgaaaa aattgacctg
    19681 caacaatatg gaattgaaca taatcgctgt attgatgacc gcttgattat tgaactcttt
    19741 tctgctgata ctgaattaat gaatttcctg ggctatagac ccactgacca gacagatttt
    19801 ttatctgacc aaaacgaaaa atcttcctac agctatgaaa aattgaaaga taaaggacaa
    19861 cgcaaattct gcggttgcat aaaaagtaaa gatatcggtc aatataatac ctgccctaat
    19921 ggctgtgtat attgttatgc caattccaat cctgaaatgg cttggaaaaa ctggaacaaa
    19981 gcacaaagga aacaaagtga cactatcgta tgacataaat aatgatccct tatttcttgc
    20041 tctggataag atagaaactg ttacttttaa agatattgta gaaaaagcaa aacaatatca
    20101 agttaaagga gtaaaaaccg acagaggtaa aaaaatcctt gaaacaccgg aagaatgtaa
    20161 tcaatacatt gttggatatt acgttaagca taaggacaaa atgtgtgata gcttagctaa
    20221 actatttgat tacaaaggtc tatataacta tgatttgaat aatattatta tctatgattg
    20281 ggcttgtgga cagggtttag ggagttggct ttttttagat gaaataaaga aaagacattt
    20341 aaaaattaca gtttcggaaa ttatgttgat tgatccttcc gatattgcca tacaaagagc
    20401 caaaaaattc atcggatata aagacaagag cattaaactt actccaatta taaaggaatt
    20461 taaagagcta tccacagagg aaataagaag agatgctgat acattacgaa ttcatatttt
    20521 ttccaatata ttggatattg atgcaataga tctaaacaat ttgtcccaat tgattattga
    20581 atcccaaaac aatcaggatt atgtaatcat tgccataagc cccttgaaag aagataggtt
    20641 aaagaacttt atagatactt ttcctgataa aagtaaagaa aaaatatctg taagaatttg
    20701 cacccaccaa accaattgta atgattgttc tgaagagaac aagggattat gttctataag
    20761 tattcaaaat agaaatacta aacggataga atatatttac ctgataaaga caaaacatca
    20821 gacaattaaa cctgtgtata tcattccgga aaaggaagaa tgggtagagg aagtttttca
    20881 taaagctcag gcagcttatt ttagtgatcc tgtaaaaaaa gatatggaat taatgcaaca
    20941 aataaaagat aaatcctact ttcaatatga aattgtttcc ggaacttctc aacctgatga
    21001 aatacaacta tctgttctta atgtgatttt aaaccaaata gtaagaggaa ttccaactta
    21061 tcttcctata gatattgaaa atctgttagt gaaccaattc agtgatctaa tccgaagaga
    21121 atctaaatac gaaagtattc aggatagctt tataaaacca gatatattct ttgatccttt
    21181 attacaggca gtatttattg atgcaccaat ttatatggct caaacccaat atgctatcct
    21241 tcaacacata aaagccaatt tccatccaga tattgttaaa ttcactttat tagaaacgga
    21301 taaattttat gcccattttt ctatgctaaa cctgcagaac atttttcata actttatgtc
    21361 tcttattccg gatttagagc taccccaaat agattataca gtatattgcg acttttccaa
    21421 tgcaacatta gttgattgta ataataaatt tggatttgct attaaagaca taactacaat
    21481 aaatgaacag gatttatctg atgaggttat tgtttattgg gatgaactgc cagaaatgat
    21541 aaaaaacaaa aatcatagga aattcataaa tattaaaacc cttactacgg attattatga
    21601 ccttgaaaaa ttccagagta gcggactaat aaactacggt gagttctttg aagatgagca
    21661 caagataaaa caggatgtgt ttcactcctt gcagtatttt ttacaaacaa tctttagaaa
    21721 agaggatttt tggcccaaac aattagaaat tctacaaaat actctatctc taaaatctat
    21781 tatggcggtt caaacaactg gtgcaggtaa atctttactt tatcaattca gcgttttgat
    21841 gcaaccagga tattgtgtgg ttattgagcc cataagatct ttgatgaaag accaggctga
    21901 tgaactggaa caattataca tagatgctat ttacattaat tcagatttgg atgcaacgga
    21961 aaaagaaaga agattgtatg aatttacagg aggtaaatcc ttatttttat tgatatctcc
    22021 agaaagaatg caaatgaaag atgtaaaact gcaacttgct tgtatggata tttatgatag
    22081 ataccattct tactttgtga ttgatgaagc tcattgtgtt tcagaatggg gtcacgattt
    22141 tagacccagt tatttatctc tggcaaaaaa tgcccgtgaa tatctgaaac ctaaaaataa
    22201 tataacttta ccaataattg ctttaacagc tactgcctct ttggatgttc agcatgatgt
    22261 gcaaaaagaa atgacttcca gtgaagataa taagccagaa attattgccg atgaaattat
    22321 cagagaagaa attgattatt ttatctttaa agaacaagac gatggaaaca aaagaaagga
    22381 taaattgcat agcttacttg aatctctaac ctcagaatct caatccaaac ccttgtccaa
    22441 agataatgcc gccttgatat tctgttccta caaagcgggg aaaaatggag ttaagggaaa
    22501 taaccaagat ggatattatt atcagcttaa agataaatat agcaatatcg gagttggcta
    22561 tttttgtgga actgacggtg ttacagagga agaagtgaat cagatgacaa aataccaatc
    22621 tgactttaaa caaaataaga tcggaattat ggtggctact caggcctttg gaatgggttt
    22681 caataaacct aatatacgct attgtttcca tattgattta cctaaatcgg tagaaacatt
    22741 tgttcaggaa tccggaagaa ttggaagaga taaagataaa tccaacagtt atttaatcta
    22801 tactgaggga gattataaat ttaaaaagaa gttattaact gatgattact taaacaaaga
    22861 gatagcagaa gaaatattta attcaatatt ggatttaagg acagattata atgaattaga
    22921 aataagttac cttttgccaa aaatcaaata taataaaggt acttataaat tcgagcaagg
    22981 aatatatttt aataagagaa ttgattcaaa tatggaagga tcgtgttttt ataatttatc
    23041 atcgcataag ttaattcaag tagaaaaaaa accaatggat gaatatactt ataatcaaat
    23101 agatgaaata ataaaaagaa ccaattttct tgaattaatg cagaataaaa gacaatatag
    23161 cttaaaggac ataattgaat caaacagcaa tataatatat catttaaagc ttcctcatca
    23221 attattaata aaagaactaa gaggtaactc caaaatacaa gcatatctaa ctaatggtag
    23281 atataagaat aatgaaaaac cgtatgaaga atataagata ttaatttcta aatgtgaata
    23341 tgtactctat aggatgcaac tattaggcat tatagtaaca tatagccgaa attacacaga
    23401 taatatattt gagtttgatt ttagaactcc acagctaaaa gaaattcttg agcattataa
    23461 agattacctt agaaaatact tatctgaaaa agaattaaaa caaaggattg ggcagataag
    23521 tgaacagctg aaagacaaaa gcctgatggc aggatattta gttatcttta atgacctttc
    23581 tgcatatttt gatgatacgg tctataaaaa aaggaaacaa tctttgaatg agatgtataa
    23641 catagcagaa aactttcaag gaagaagttc tgagataatt agaaagcatt ttgaccttta
    23701 tttcaattcc aagtttatgg aagaactcag ttcaagaacg gatagcggaa agattgagga
    23761 tataggtata atagccgaat ttctgcaaga agtggataat ttatccaggg gctccagaat
    23821 tgagaatctc aatcatttgc aaggtgcttg tgccagatta ttggaagaaa gtcctgataa
    23881 ctatgtttta tgtatattaa ggtcttatgc tcaactatgt tctgctgagg gcaatgaaaa
    23941 agaactggag tccggcgtgg aaaatttaga gaaagggtgg tataaaatgt tgcttgatta
    24001 tgaaaacctg gataagatgt tattacacaa tgaagtatat ttaccttatt tgacaggttt
    24061 aaaacaggat ttatccgata ctgattatga aaaggtcttt aaaaaactat cagttttata
    24121 tgtaaaagca atgcttcaag attttaataa atatttaacc agttagggag gaaaaaatgg
    24181 aacaatatga aaatattatg aacgaattga attctattat tgctgaatac aaagataatt
    24241 tggtttcact gagagccaag tttgaggata tcaaagctat tattgataat ttagaaagga
    24301 tgaaaaacca ggcatctaaa actacagaag agatatctga actggaaaag aaatatgggg
    24361 tattggcaga attgtataat aaaaccgatg ctaaccttaa tgaaactatt gccgatctat
    24421 ctacccagta tcaacagtta acagaaaagc aggaacaaat cgtggggata atggaatcat
    24481 ttagaaaaac acaagctgaa tatgctgctc aacataattc tgcaatccat gatttaacga
    24541 ttcaagaaaa agcggatatg gaaaacttgc ataactacat ggaagaagta tctggtaaga
    24601 ttaatgatga agtaataaga gcattatccc aaaaaatgga tgtaagtact cagtttttaa
    24661 atagaaactt aaatattatt acaaagaatc agaataaagc aaatagtgaa ctggaaaaga
    24721 atatgatgga tagaatacaa aaatgtgaga agaaattggg aagcaaaatg ataaattgct
    24781 ctattgtaat aattattcta ctagctgtga ttttttttct cattattata tataccaatc
    24841 aatattcagt ttactaagta tgtttcactt attaaatgcg aaagaccttg tggcagtaat
    24901 tagcaaaata tgcgagaaaa gttattctga gagagcttat ggctacaata ttatatccaa
    24961 cttatacaga agaaatatat tatagataac accgaaattt ggttctcaaa ttaagatttg
    25021 gcagatagtg gaagtattaa ttatttatag atttttagtt tcttgggcaa atccgaattt
    25081 gccaatccat aatgcattat ttatacgata tttattgaaa agacataaat acttgactaa
    25141 taatatatgg attcctgctt tcgtaggaat tacaagtaac ttgcattcaa aaagtaatat
    25201 taggtaaaac tgagcatcta ttgcttttgt attatatcca atactttatc ataccataaa
    25261 acaacatcat taaactgata tagtaaggaa ttaactgtgt tgttagttaa attataggta
    25321 ttgatatttt ctatgtccac ctctttacta atattgtgaa taccaaattc acttgaaatc
    25381 ccgtatattg aatagcacgc atttttaatt atggaattat atccgatgtt gtttctttta
    25441 ttataattgg ataatccgtc attactgata aatttattta ttattagctc atttttatat
    25501 tttttattcc aatattctgc atttggttcc tttaaattat atgctatatc gagcagtata
    25561 ttttcaagaa tattccttaa tttagatagg taatctttcc atttatcttt attcgcttct
    25621 ttatctttta tcatattaat atattgttta actatatctt catcaagatg ctttctaaga
    25681 atttcacatt gttttgggta ttgattctgg attttaaagg acttcaaatc tgataaaatt
    25741 ataccgattt tttcctcatt ttcagaaccc ttttctatta tatgctcttc attaaaatta
    25801 ccagaacgaa tcaaggattt catatcatca agcactctta atccatcatt ggtattagca
    25861 gtaagaaaat agattatttt ctgataatca gagtttaatt ttaataaaag acataacaat
    25921 aaatcgcctt cccgttcata atattcttta atttcatcta ttgacttata atcatatttt
    25981 tccaatagat taaaaatccg ttcaaggtct ttgtcataag gaaagaggct aagattacga
    26041 tctatgataa aaagatcata attttgatga tttttaataa ttttttctaa agcatccggg
    26101 aaattataag caatatctaa ggaactgtta tagcgacatg catcaattat atcttgaggg
    26161 tttaataaat cgctattctc aaggtctttc aatttgttcg taattctctt gtcatttaga
    26221 ataggacgaa agaactttat aattgtagag atgtttttag ctatttcatc ttcaatgtat
    26281 aaaactttag ccattgttcc tccataaatt cataaattcc aaactaacgg tatagtattg
    26341 tttatctgtt ttaacttcag aaatacaatt aagagctctg gcaaaattgt ctatcagtaa
    26401 atttccaatt ccggttgttt ttactgtaat attgggatta aatctatttt ttacagaaaa
    26461 tataagctta tcaatatcgc tttttagggt aatactaatt attctatttt cccgaggtat
    26521 ataggaaaca tattttacag cgttaaatat aatttcttga aaaagtatca tcattttgat
    26581 agccgaactt ttgctgttac ctattaaata agctcccgat tcatctataa atatatcaat
    26641 taaaaataga taattttccg caaatttcac taatgcttct ttggaattat tttcagaaac
    26701 ggttttccaa ttattctgaa tatttttaaa ttcctcattg gataaggtag aagcaaaata
    26761 attaagggca aacggagaaa aataacggaa gtcaaacata ttactgattg aatacttcaa
    26821 actgtcatag atcatatcct gaagagacat tctttctttg tcaggatgag atatatccca
    26881 ccgtaaatct tctaaagaag tagtataaga ataattgatt gcatttacaa tttccctaat
    26941 caggtttgcg ccttttatag cgttgtctat aacaactcct ttttctggaa catcttccct
    27001 taagttgata agcggatcaa taactgcctt aagtaaattt ttaatgctat gagataaatt
    27061 agacaaaata cgattacgct catccagttt tgcctgcatt atttttttct cattttcctt
    27121 aaagatgagg ttacaaagtt gattatctaa accacgaata aaaggtaaat agctttgtac
    27181 tttattgtct ttatcaattt cttggtaagt gtaacgcaat aactccaatt ttacttttag
    27241 atcatccgga ttattagaaa aagtatccaa ttcctccgcc acttccttaa atttataatt
    27301 aggtgattta atttcaagat atttagttat aatgttatgt tgtggactcc aatcacaaat
    27361 tcctttttca aatagtttag ctaaaaacct gatgtataat aagtcactca tcttttgcca
    27421 gttcaaactg gaaaataaga gattgatata ggaatcatcg taaatttgta catagctaaa
    27481 cctttttaat aatgaaaagg aatataaatt tgcaatctct tcaagtaaag tgtcaaaatt
    27541 gatattatct ataattttac ttgcttttgc attattattt atttcaatat atgtgcaaag
    27601 ggcaaaccaa ccaataacct gattagctaa caatacctca tcaaggttgt ttagtatagc
    27661 attatattgc tttatctgga taagtttctg taaataatag gataaacata ttcgtagtcc
    27721 ttgtttataa agattgaaca cttctttagt atatttatca tattgttcgg taataaacaa
    27781 aagttccagc ttatgagcat aagaggaatc gggtatgtta gtttcaaaaa tgtcgctagg
    27841 attaacaaca ggtggaggga agtttttaat catttgctca agaacatcaa ttgctttgtg
    27901 ataatcctta atttggagat aaatctcata cttatgtcga agataagatt caagattatt
    27961 atcatcttga tatgcttttt ccgtgcttaa gagagcttca tcccattttt ctaattttac
    28021 ttcaatttct ctttttagat tataccattc cgcattattt tcgtaatcta tatggtgtga
    28081 aatatcttga tatacttgtt gtaacttttc taatagcaaa ttattgagat tcgcttcagc
    28141 tttatatata attgatttta ccatttcttt ataatatact aatttatcag tgagctgtat
    28201 taactcataa agggttttaa atttttcttc ggtgatatat ttttttttat cttctggaat
    28261 taagggtttt ggctcctctg ataatttatg ttttactata gtattattat caaggggttc
    28321 ttcaatgcaa atttcttcac tattattgtc caagcaataa taacaataca atcttatgcc
    28381 aaaatctgca tgtgcaccat ccaatacact aagtatttgc cattttaaaa ttagattatt
    28441 ttcacaataa tttagttcta atgctctatt agcatcttct aaagcttgat aataatcctc
    28501 aagaagagat ttttcatttt tggaaatagc caaagctctc catctgtaat aaaaatcata
    28561 ctcattttct ttaagagctt ctttcatcca atctaatatt tccttaaatt gtttattttc
    28621 catataattc ataaacttag catgcaattt ctcatattcg gaattcttaa taggtggatt
    28681 tgttttttga ttatttgatt tattcatacg aacctcagat aatttttttg aaaatagcgt
    28741 tatatttttg gttgcctaaa ttgatgataa taatatttga tctagctctg gttaaactta
    28801 ctccaattat tataacatca gagcaatgta tggacatccc cttaatgaaa ttgataatta
    28861 cctcctttat gtttagtccc aaatgttggt gtaaattcca actcgttgtt tctcaggttc
    28921 tttgaggaca tctgttatcc cttgttactc acaatctatg taaggtaaag cgactgattg
    28981 cattcttttc atctgataaa tcaatgtcaa gttctttttc aaaattaagc taccttgctg
    29041 agttgcctca aatggtatag cccttttctg gttttgtctg attcattcca ttggttctta
    29101 aagataaagt ttccagctta tccagtcctc tgaatagctt gcaggaaagc aaaaaataac
    29161 cgcagacaac tcttttcatc tgtgaagatc ctgttgcatt agtctcttcg cctgaactct
    29221 tcatttatac gctccagtga gtttgtgcat ttgattctac tctaatgtac tacagggaaa
    29281 tcaagatata cctatatctt atggtaagtg ctatcatgcc aatccagaag tttgtccttt
    29341 cctttggatt gggctatcct agtaagctct ttccacacca atagaaaata ttctttcgtc
    29401 ctggatccta ccaggtcttt taggaatagc aataaccaca gaaggtccct cttggacacc
    29461 ttgctctgtg cattgtggta attcgttcta ccttccacac actgataatg ctccgctcca
    29521 aagattgagc taatctcagt ttaaatgata ctttggatgc caatttccag cactttcact
    29581 aactctcaag gaagcattgt gcaaaataac ttgaaaaatt cctccttctc atctttgata
    29641 atattctgtt gagtcatgat ttgctccttt ttctttaatt ttaattaaac catcaaaaca
    29701 tataggggga tctggctcaa cctttttttc attccgttca acaattttac accaaccttt
    29761 gagacactaa ctggaaacca aaattaatga gggaacttag tatatcatac tttatagcaa
    29821 atttatagaa tactttaatt cagaaacctg ttttctaaat aaattacagt aagtgcttct
    29881 tcctttttta cttgacggaa taaagccaat tgaaagaatt gaaaaaaagc aaaaatgaat
    29941 attaggacag gtaatgaaag atagagggaa tatcctggaa cttctaaagc aagaagtatc
    30001 tcttgcttta ggttgtacgg aaccggcagc tgttgccttg gcaggtgctt atgcttcaag
    30061 tgttttaaag gatacaatta tctctgccga tttgattgta a
//
LOCUS       DAOPFL010000009        27823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1683_length_27823_cov_105.079624, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000009 DAOPFL010000000
VERSION     DAOPFL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27823
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_27823_cov_105.079624"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..425)
                     /locus_tag="PLG69_01630"
     CDS             complement(51..425)
                     /locus_tag="PLG69_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42241.1"
                     /translation="MTNEEQLELIKLKKENLRMQKALDDSQLHNLSLETIIEIVENSF
                     GEKKIFRFATIRGTREKNALFRIPMNLKVICKALNYTRQAYYKIAKKMSLLQKKHMKI
                     LAQVQLIRLQKKWVVKVKSIPS"
     gene            complement(523..753)
                     /locus_tag="PLG69_01635"
     CDS             complement(523..753)
                     /locus_tag="PLG69_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42242.1"
                     /translation="MDQKVIQKGMYNRYSVAFKMKIVEEADNGLISMNAARKLYNIHG
                     KNTITEWVGAVPKIKVSFKTVNISHFKEQSPL"
     gene            complement(939..1505)
                     /locus_tag="PLG69_01640"
     CDS             complement(939..1505)
                     /locus_tag="PLG69_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPI42243.1"
                     /translation="MKKDIILAGVGGQGILTIATILGTTAIKRGMNLKQAEVHGMSQR
                     GGDVQSHLRLSDETIWSDLIPFGSADMILSVEPLEALRYLPYLKDDGWIITNSTPFKN
                     IPNYPPEEEIYKEIKKIPNYVLLDADTKAKEQNASRSMNIVVLGAAIKHLGFSKEEIE
                     EAIKTIFAPKGEAIVEANLRALQAGMEG"
     gene            complement(1502..3109)
                     /locus_tag="PLG69_01645"
     CDS             complement(1502..3109)
                     /locus_tag="PLG69_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPI42244.1"
                     /translation="MPKLMLLGDEALAQGALDAGISGFYGYPGTPSTEIIEYAQRSKQ
                     AEENNVHRTWSSNEKTALETALGMSASGKRAMVTMKHVGLNVAADPLMNSAITGASGG
                     LIIAVADDPSMHSSQNEQDSRYYGHFAMIPVLEPSNQQECYDLAFYGFELSEKYHIPI
                     LLRLTTRLAHSRSGVTRRNPLPQKELKKPEDIFQFMLLPAIARKKYQHHLSLQKDFLA
                     ESENSPYNVYTAERKNTKLGIITTGLAYNYLREAYHGEEIPYPVLKICQYPLPEKQIR
                     QLYETCDELLVIEEGMPFVEEQLKGLAFPGLKPIHGRLDGYLPRAGELNPNLVAKALS
                     LPDTIGRPVPDIVVGRPPALCVGCPHSDTFLSLNEVMAEYGRGNVFSDIGCYTLGFMP
                     PYNSINSCVDMGASITMAKGASDSGLFPAVAVIGDSTFCHSGITGLLDCIYDKANVMI
                     IILDNATTAMTGGQNYTGYGKLEDICLGLGVEKEHIRVFVPLKKNYPEMIQIYKEELA
                     YNGVSVVIARRECIQTLKKKNKMEIQE"
     gene            complement(3136..4599)
                     /gene="malQ"
                     /locus_tag="PLG69_01650"
     CDS             complement(3136..4599)
                     /gene="malQ"
                     /locus_tag="PLG69_01650"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPI42245.1"
                     /translation="MRKTGILLHISSLPSDFGIGDFGPEAVRFAEYLKAEGYTYWQIL
                     PINHCGYGNSPYNPLSAFALNPYFISPELLYQQGYINQLMLEAVKIPRSNVTRYEQVY
                     ILKDKMLAQATANWMLANRTDDYIEENAPFLKPYLTFLSLSKFYDDTCWYNWNEEHRH
                     YSEKLYQKVRLMNSSYFNLRAATQAMLVEQLSWFKERLQELGIILIGDLPLYLAYDSA
                     EVWAHPEYFDLDEQGRRLHFAGVPPDAFAEKGQLWGNPLYRWDVLKQDGFQLFMERIK
                     QALGYLDILRLDHFIGYVNFWQVEPELDANGQPVLPKDAIKGSWVRALPEDFFSALLQ
                     RFSIKRFIAEDLGILNDDVCRIREQYGFPGMIVLQFCFEEGVPDVKKYPPERWLYTGT
                     HDNQTLRGWFESLSPNSPSYKHLQEYCALHQFTDCQAHLNAENIHLIMRNIALSSSCQ
                     QVIFPYQDILGLGDEARMNIPGTALGNWQWRLENLNF"
     gene            complement(4599..5600)
                     /gene="holA"
                     /locus_tag="PLG69_01655"
     CDS             complement(4599..5600)
                     /gene="holA"
                     /locus_tag="PLG69_01655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPI42246.1"
                     /translation="METSIFALDFTADKVRLGSSYLLIGTDSYLVDRVLDTIRAKLKK
                     KEKADTFIIYGDEVKSPELADLLDTYSIFSAAKLIIIKNAESLKTKELSTLADYFSSP
                     SEIQSLAIVTEKADARHENWKKIKAGTKLITCDPPPYGGAIRAWLDKALKDINKKMTD
                     KAIEEFLNRIELDYYNAANELTKLDLLTRDRTTITEEDIMQSLGTTRSGTLIDFYRAL
                     GRKQAKQAIEAMEKMLFADWKPLQILFQLTRFYFNIWKILLLKKAHITDNEIVSKYIT
                     DFHPNQRREFLDLSRYYNINALEKIFALLLETDQQFKLTVAEPQILLCNCLLKILDA"
     gene            complement(5600..6415)
                     /locus_tag="PLG69_01660"
     CDS             complement(5600..6415)
                     /locus_tag="PLG69_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPI42247.1"
                     /translation="MSLIQVILMGILQGLTEFIPVSSSGHLVLAQHFFGIGDSENIVF
                     ELFMHLGTLLAVLVFFRKTLWELIKSLFSWGNTVNREQHRKNRTLVYYLIISTLATGV
                     IYILFGDFMEAVFNMPMLVAIFLLVTGVIVFVSDYFNDKGIPACNIGFLQAVLIGIGQ
                     GVAILPGISRSGTTIACSLATGMKRKDAAQYSFLLSIPAILAGNLSQYKAFANLKPQL
                     LINYLAGFVCSFLVGYLVIAFLIRLIEVSRLKYFAVYCWLIGLLSIVLIILGF"
     gene            complement(6426..7715)
                     /locus_tag="PLG69_01665"
     CDS             complement(6426..7715)
                     /locus_tag="PLG69_01665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPI42248.1"
                     /translation="MPKVRKKKLRKVKRRRKGGNPGIFILTGFICCFLIWIIIKEPAD
                     KNSELSKAETVSKNSELPVKPKKKTKKEVKAISPNASKILPKDKKAQETTIDNTKNTP
                     ESAITTTIEKLGIPKSYFRKRNTENLTIFNVPIDKSEMDLVYANMIFKGELERNGAKF
                     IKGMDSAGKQTLLFSFPDTAKQYEINIFYDNNLYRNKQKKRTITIIVDDFGTIGGDLL
                     TGFLALDKEVCFSIFPEEEYSVQTMKLAKEQGRLTLIHIPMEPIGYPEVNPGKNPILV
                     QYDERQIEKIMTRFIEQLPYCEGANNHMGSLATTDEEVMNAVMTILKKHNKFFLDSRT
                     TNVSVAYSVAQKNHLKSYRNDLFLDSPNISQSTMDAKLNQIIELSNRNQNVIAITHCH
                     NYDKLDYLKRFINRLKAAGFTLIPLTDIDKYNVPEIL"
     gene            complement(7699..8517)
                     /locus_tag="PLG69_01670"
     CDS             complement(7699..8517)
                     /locus_tag="PLG69_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42249.1"
                     /translation="MIKSILNALFGDYNGIQVFVAPITLLYWIDSGSLLSSATSLLSF
                     RMHYLPLLAFLIIFVFSVFMLIKIKLLYNCNNSEYLDMVIQFNVSVMALVLIGLIIYA
                     ISSFLAYFYGIKGTVKSGLLLLFKLYTVFLILYHYLFNVVLTPYYQKQYGHPRALKAF
                     LSWARNNKFLLFRYILLILLVVFFAVRFYQLILRFALMPLIGFIDKYTGISIKFKLYP
                     FVMIEDIFVNVLVLTGAFLVSNLFFFPLIWVLKYLVNRFIPFKNLLRTSYAQSA"
     gene            8987..9385
                     /locus_tag="PLG69_01675"
     CDS             8987..9385
                     /locus_tag="PLG69_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI42250.1"
                     /translation="MKTQIWVNNPITNEIAFNTQFFAEPGALIPFSVQLPVSVEENIV
                     SIPAGKLNLYPSVLNPHWNESIRISYDTKLTGKAELVLYDLKGRHLAEKVYDQNEIEW
                     QLPKLSSGIYFVRLTNQGKALGTAKLIILK"
     gene            9382..9954
                     /locus_tag="PLG69_01680"
     CDS             9382..9954
                     /locus_tag="PLG69_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42251.1"
                     /translation="MKYAFLLLLLAEIMMLSALTFEAEELNFYLENNLWEIDGLFHFA
                     NYDSLSTHKLIFFPVPSDSLCLPPKIKAIAIQDSNGAEVTMLNQAKKGFTFLLSLPAL
                     SFCSLHLDYTQTLKSNYAKYIITTTNSWGRPLPYAKYTLHPGINVQIRSLPFSVQKQE
                     GRNYIWEFFDFAPEKEFEVTFQTIPAEDQN"
     gene            complement(10524..12002)
                     /locus_tag="PLG69_01685"
     CDS             complement(10524..12002)
                     /locus_tag="PLG69_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42252.1"
                     /translation="MKRTLLILTLALTLTLLFSQTQELPDLTVSGESSFKPFLYKRSL
                     LFSPELNMGDSLPAFVPPGVPLLEKPKPIPAMQHRSYLQFEGNMDFGLNSFISYYPDS
                     FILNALTYTLDMRSPISELLSVHNNLFLGTELSSHFPLSFRFQNVSSQADDFDNTVLD
                     FNFSHHRPDLTIGNVSFKDISAQLGYNYIYQKNLNKPYKCNYLNVYLASCLEEEILNW
                     KTKFMVKSGDFGIQIAPVYNGDIMDISKPGIHLLADAYTIVPSLEFLYRYPITGWGVL
                     SLSNVPLLEGNDYLSFLEATPWISFSDAHKLKKTPLNFNAGLEYLYPQKINFSLGRLN
                     INNNLRYEIDSPVLVSTNNYRIPTLRYTDVFSNLTSVEAFFKMDKLNMHQGLELELAY
                     LTESDYTRAPYRPLLNLDSRFAYGYGNWYFNLDILQHYFTKDHRGHDLPEAIILNLGA
                     EYHKDNSAAYAQLANLFNRKEWVFSEQPGKGRNLYIGLKHRF"
     gene            complement(11999..14929)
                     /locus_tag="PLG69_01690"
     CDS             complement(11999..14929)
                     /locus_tag="PLG69_01690"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI42253.1"
                     /translation="MKYFLFTLLTLTLTCFCFAEFIVPQNNAPLMAATNSENYAYRYI
                     VELYNVGDTLNLTKEITQFKATYPESVYLPYIKFIEGNLAMEANAFSQAIDIYNSLLE
                     TNLNQNVRSEIILNYAYCLTQLKQYDSALKLLQRLETETGNPTYLELANSLRGDIYFQ
                     LGQYYSAEKAYLRAVKAFPDNEALLFSLFSTFLALGKDDQAFTLLKNQSPKDTYFINY
                     IQSWLEYLLENEYYNDFDNFVESYKSDEIAFVPEIVDIRIRRALRTSDFDQISRLLEG
                     LNNNTPQFNYYRALVLIRDGKENQADSLLAELVNSTQPEIAVPAYLERLKLLFKKNPQ
                     NAIEQLQTYLKQTPNELMKAELYYTLGYFYFQQKNYTEAIRQLGQARNYETSRELNSR
                     IDFLIAEAFYFAGNSNLAKDAFNRYLSRYPSGNKADKAYFYLGYLSFLEKDYTEAKNN
                     FQELINLYPESFYGNEALYYLAEMDFYLANYNLALKKYLYLYEKNPENEVIALRIAQI
                     YFYLGDYDQSENFLQNLVPNYDICLLKGNIMLAKKNYSPALEQFILAEGFATDNVRKI
                     EAQSYRALCLYQMKRFKDASTLYLKLSREKESPDTYLFLAAKSAYAAKDYHLALELYN
                     NFIDKYPESSHFLEALTDIANTYYNMGNYERAVNDWINILTRFRNTTKFTENQLATIH
                     DALVGLELALKRVNNKDELLNDLLVLPDTFFSEYIKFELNYILMKVYADELKWADLIA
                     TAEKIRTEFPEQKHEDIELLMATALIELNQYAEADTLLAELYAQSKNNEALLKWAELE
                     YITGNYDSALEKYKLAYKNNPDGATWVKLLECSEANNYKDFDKLWSLGSNYLKNHPEV
                     NVIRMRQLYNAQRFEEAEALIEFIINNSLSTLDHCNAFLVMGMIDYHRHSYLSAISTL
                     KKVILLFPEYKEIRSKAIYYSVLSLLESGAKTEAEALFFKYREELDEAAKMELTDVFL
                     EVNQ"
     gene            complement(14946..20429)
                     /locus_tag="PLG69_01695"
     CDS             complement(14946..20429)
                     /locus_tag="PLG69_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42254.1"
                     /translation="MFIDHRYEVLERLGSGTWANVFKVRDIRTDKLFTLKLFQYVSSE
                     ELYSRFSAEDMHRITQLEHPNLNRVVDFGHIADHIYFISEHFDGKPLSRFKFSKSRIN
                     LIYDIAVQISYALNALHTQNILHKDLKLENVLYRLEGNSIVVKLIDYGFSKIDTPKDS
                     QAVSGSLPYLAPEVYLGKSPSKASDFYALGVILYKLTTGSFPFSVDQINALITGGNQY
                     FIPNFPSELNKDIPLPLEKFILRLLDRNPDNRFTNSEEMVDYINRIQPVQYPFSVSWS
                     LINTLRFNSYLVREKYAHQLLDYIPAVENGNGKIISVMGGDGLGKDSIMSLFRYHLLS
                     GKYFIFDYTCSKTDHEAFFALIKEFVQSLSADEIQEYTDLQMISEKFRRYLFESEQEA
                     KTISQTTSELKLDFESVKSLLIELSSNKSIIFIIRNFQYVHHNTIDFLNFMSSYIVKH
                     RILIAITCNEYNKINQIEHTVMLHIPNLNLEETKKYINRLLANITPVSNQNGVPNAFI
                     EEIYKRSAGNPQFIVEILIDLTQRKKLYYNKELHYPLNLSDYQLPTRLLHSIYSRLSH
                     LRDVNYLHLQKLSIVQTPLTRELMLYILKIKDAELYSLLNDSIYNEILRKHRKYYYFT
                     FIEAKQRLFGECNERRQILVSQRVLKFYANETILDFETCKGLIANAFLANDYSCARFY
                     YLKYYEMLNQENEQEQAFEAILNVLNLDFNPALQVPLQNIIADIAIFQEKMEMTGFFE
                     RAGFILQRISELPDFFEKHYLLGTVDFLAENLRQALKYFQTAEKYVHTGKHQLLVWLA
                     YAQIYTKLDPSKMKHYIDKALKQEMTLELQIAFVDRLAVYYSLNKDFDAAIKTIESFL
                     EKLPTENNTKVMIRLAAMHNDLGVFYSTQKKIEEADEHLVTALNIWKRYNIKRYLGLI
                     YNNLSDLYLKQGLTVIAEHYSELAYQYSDDLNLTMSKALALLNQGEAKIKMGDFSTAE
                     QKLLKCQELVLSKGSDNYLQSVKRNLALAKSKIKGFGHYYTFIWENEPELLNGQIKEM
                     SPLVKTYFYYLYETGNAKKLRRLLTKNVHINYKHIHEEEFYHNALALTAMVEKDYERA
                     LNELKIALRHAGEINNNYAIAVFYVLQIRCYYGLKDVELARELIEIALPVIKNNQYRY
                     WQCELEVLALKLDLLNPDIPLRVILRQVNEYLIAWQEYEYYQLNVELYQIKIQIFTEL
                     KQEELAQAEFENYKAYLEKITENISPDGRQNYLTINLYFLKNLKNFNFLPVVSRSRDL
                     VKQWNDMLYNITNIHNIERIKFLIEKGLIQVLAPWQFWLMQYSEKVSSYTCFQNYNTG
                     KDNLINTQLMPFIERAYKTDNLVVLEQNNRHLMIVPFQIGSKKIGFLLVSDKGEMPFT
                     KQEIYLMRSTKQHLSALLVRIEDFSQITQRIEKMNQLMSITHELMRIVNLNELEPAIV
                     SACIDFTNSTRGFLIKRDSDGAIIYKVQLNAAKQILPTVSGVSKTVLSLSQNTQEIVS
                     TYNAVEDNRFKSAISVQDYVLHSIFCAPLVVDNNVYGYIYLDNLNDNTREMYLNTELI
                     KLLLEQITIALKNAMLYENLLQKNNELNTFETLKDEFMAIVSHELNTPLTTLQGYVTR
                     LKRNLFADEEEKRDIINKIENSVKKLILTINDITTLNTYNLTKSLPLTDISVQEILEI
                     VHQEVQILSRNRRMFIKLEIEKDLPPIKANWEALHLMIYNLVLNAIRFTNDFGTVIIG
                     ARKSAFQSEKIDGKEALVLYVQDNGIGIPEYQLQNVFRTFYELNEIYAHKSGIIEYRS
                     SGLGLGLSTSKRIAELHGGKIWIKSKENEGTTVFVSIPLKQNK"
     gene            complement(20473..21360)
                     /locus_tag="PLG69_01700"
     CDS             complement(20473..21360)
                     /locus_tag="PLG69_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPI42255.1"
                     /translation="MPNKQIQNKIEYLGFRIALAGLKLLPAKAAKSVLKGLFWFGGWV
                     IGIRKYVALKQLSKVYPDKSKKEKAEIVKKLYRNMALTVYESYLMDDDTLYKNIRIQG
                     REYIDNALSLNRGVIFAAAHYGNWEAARILPKAGIPISGIAKPQRNTLFDNYTNAIRE
                     RCGMRIINMKRGLRDIIHEFKENRIVAILIDQNAGSSGLVMDFLGFPASHWKGVAKLS
                     LRYKIPIVPSFARRAEDDRIQFEFFPPILHEELEDTEENYKIVLEEVDKILENQIHKH
                     PEQWFWVHLRWKHSYNMLA"
     gene            21662..22270
                     /locus_tag="PLG69_01705"
     CDS             21662..22270
                     /locus_tag="PLG69_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPI42256.1"
                     /translation="MNDQLQDLLKRVYEEGVAKANAEAEKIISQAKEQAEETIAKAKA
                     EAEAILKDAEKKAEDLKKNTQSDLNMAYNHTLTALKQKITDLVLSEALNTKLNDAFND
                     TEFVKKLILETLTAWRENTSSGTIIISEKMKPHLDEFYIKSLKDIFDGKLKVDFSPVM
                     KQGFIIAPEDGTYKIKFEGDDFANLFKNYLRPRTKEILFGNQ"
     gene            22274..23161
                     /locus_tag="PLG69_01710"
     CDS             22274..23161
                     /locus_tag="PLG69_01710"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPI42257.1"
                     /translation="MKQEYYYFVSGLPNINIDDTKLPWTPEEFLAEAKTQVSENDYHL
                     LEILHLPMDLDNLLRVLYKTNKDRNMEGLYDDAFWEEYLAFLRDVIDNPEVKTPIEFS
                     ALPNFIKEIVLNTLKQEEMQPLPQTELALLKKFYAWTAKSPNDFIRDWFAFDAHIRNI
                     LTAINGRKFNLPYAQYLVGEGETTEKLSKSHTADFGLGKEDELYNALTRIYEQNNILY
                     RERGYDILRWRWIDDYNFFNYFNIDRILGYYCQLRILTRWIKSSPELGKEVFNSILSD
                     LNNSFSFPADFNIKSTQRK"
     gene            23175..24929
                     /locus_tag="PLG69_01715"
     CDS             23175..24929
                     /locus_tag="PLG69_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPI42258.1"
                     /translation="MTTGIVKGIISNLVQVEVSGPVSQNEICYINLGKEKLMAEVIKV
                     VGNIANTQVFESTRGLKPGDTVEFTDHLLEVKLGPGMLSKNFDGLQGDLNKRKGVFLT
                     RGEYTDPLDENSLWKFTPLVKVGDKVSAGDWLGSVPESWITHKIMVPFVMEDEYTVKS
                     IAGEGDYKITETIAVLIDKEGKDIEINMIQKWPVKIPITAYVEKPRPFKLLETGVRTM
                     DTLNPMVEGGTGFIPGPFGAGKTVLQHSISKNAEADLIIMAACGERANEVVELFVEFP
                     ELDDPRTGRKLMERTIIVCNTSNMPVAAREASVYTAMTIAEYYRNMGLKVLLLADSTS
                     RWAQALREMSNRMEELPGPDAYPMDLPAIISNFYARAGFVYLKSGTTGSITFIGTVSP
                     AGGNLKEPVTESTKKAARCFYALSQARADSKRYPAVDPIDSYSKYLEYPEVIEYLNSN
                     VNPHWVKDVNHTKDILLRGKEAQEQINILGDDGVPLSYHDKYWKSELVDRIILQQDGF
                     DKVDQSTPMKRQQYMLELILEICDTDFVFEGFEEVMPYYMQLINICKQMNYMEFQTPE
                     FNNYEAELHKIVAERRAK"
     gene            24929..26251
                     /locus_tag="PLG69_01720"
     CDS             24929..26251
                     /locus_tag="PLG69_01720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPI42259.1"
                     /translation="METQAFQKIYTKLNQITKATCTVQATGVGNDELATVAGRLAQVV
                     KIIGDNVTLQIFAGTEGIGTDAEVVFFGKAPTLKVSDELGGRFFNAYGEPIDGGPEIE
                     GKEVEIGGPSVNPVRRKQPSELITTGIAGIDLNNTLVTGQKIPFFADPDQPYNEVMAM
                     VALRAQSDKIILGGMGLSNDDYLLYKNTFENAGVIDRIVSFVNTTEDPTVERLLVPDM
                     ALTAAEYFALEKKEKVLVLLTDMTLYCDALSIVSNRMDQIPSKDSMPGSLYSDLAKLY
                     EKAVQFPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLKRDTDIGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQVMNTAVRLYADAANAKTKLENGFDLSDYDIRVLDFAKE
                     YSDKILAVDVNISTDEMLDRTWELFQKYFNRAEIGIKDEFMNLYWKKG"
     gene            26248..26847
                     /locus_tag="PLG69_01725"
     CDS             26248..26847
                     /locus_tag="PLG69_01725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPI42260.1"
                     /translation="MNLKFQYNKISQLQLIKQLAVRQRALPTLKNKESALRLEVKKAR
                     DQAFALDQKVKERTAELDAFMKLFVEFDPYIVKIKNVEIKTRKIAGVKTPLLENIEFE
                     IADYNLFKAPSWYPEGIALLKEICQLQIERDFYIRKMQILEQVRKKTTQKVNLYEKVQ
                     IPAFEDAILKIKRYLEDEENLSKASQKILKSRFEQESQL"
     gene            26844..>27823
                     /locus_tag="PLG69_01730"
     CDS             26844..>27823
                     /locus_tag="PLG69_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42261.1"
                     /translation="MIEKMRKYTFVLYHQDYENFLAELQKLGVVHIIRSCDEKTESQT
                     KNLELMKEYGECVKFLTKLKSTAPKQASPLPTKALLNKINEAREEKDKLQHSIDTVKK
                     QIRDLSPWGHFDYDLVRKLKEYGLTIRFYTCLKNHFKPEWQEKYPLKIINEVGGILYF
                     VVITDNGDPALEADTFSFHQHTLQEFEDKLKELENEQKDIDDYLYSIAPTAIEMFQDE
                     LKRLSREYEFEDALQQGKYEAENTIRLLSGWIPVSLEQGLKDFLEKNNIIYVSEDAKP
                     EENPPIKLRNNWFARLFEPISKMYMLPYYDEFDLTPFFAPFFALFFGFCNA"
BASE COUNT         8665 a         5191 c         5439 g         8528 t
ORIGIN      
        1 gattcctcca attattgttg acaattatat gcatctgata gaaaaggata ttaagagggg
       61 atacttttta cctttaccac ccatttcttc tgtaaccgaa ttaattggac ttgagctaaa
      121 attttcatat gttttttctg caataaagac atcttttttg ctatcttgta ataggcctgc
      181 ctggtatagt tcagggcttt acagataact tttaaattca ttgggattct gaagagagca
      241 tttttttctc tagttcctct gatagtagcg aagcgaaata tttttttttc gccgaaggaa
      301 ttttccacaa tttcgattat agtttccaga gagaggttat gaagttggct gtcatccaga
      361 gctttctgca tacgcagatt ttcctttttg agcttaatta gctccagttg ttcttcgttg
      421 gtcataatat acaccgcctt atttatattt tggtttctgg ggctgtctca caacctccca
      481 aaagtccatt ttttgccatt ttcatgtata caaagatgta tcctaaagag gagattgttc
      541 cttaaaatgg cttatattta ctgtcttaaa actaactttt attttcggga cagccccaac
      601 ccattcagtg attgtattct taccatgtat gttgtaaagt tttcgagcag cattcatact
      661 aattagacca ttatcagcct cttccacaat cttcatttta aaggcaacac tatagcgatt
      721 atacattcct ttttggatta ctttttgatc catttttttt ccttgatttc ggatcccgtg
      781 ccaacttttt taagaagcct aaaattaact tttcccttag gggtatccca tcatcaaccc
      841 atcatccact catcctgaag gttgggttga tgatgaatta taagcaggga ataaaataat
      901 cggttcttta ataatgtaat tgatatttta cgagagggtt atccttccat cccggcttgc
      961 agagctcgca ggtttgcttc tacaattgct tctcctttag gtgcaaagat agttttaatt
     1021 gcctcttcaa tttcttcctt actaaagccc agatgtttaa tggcagcgcc taaaactact
     1081 atattcatac ttctgctggc attttgttct ttagctttag tatctgcatc cagtagaaca
     1141 tagttgggta tttttttaat ttctttgtag atttcctctt ccggcggata gttagggata
     1201 tttttaaaag gtgtggaatt ggtaataatc cagccgtcat cttttagata gggtagatag
     1261 cgtaatgcct ccagcggttc aacgcttaaa atcatatcgg cagagccaaa gggtattaaa
     1321 tcgctccaaa tagtttcatc ggaaaggcgc aaatgggatt ggacatctcc cccacgctga
     1381 ctcattccat gcacttctgc ttgtttcaaa ttcatcccgc gtttgattgc tgtggttccg
     1441 aggatagtag caatggttaa aatgccctgt cctcctactc cggcaagtat tatatccttt
     1501 ttcattcttg tatctccatt ttgttctttt tctttagggt ctgaatacat tcccgacggg
     1561 caataacaac agaaacacca ttataagcta gttcttcttt ataaatttgg atcatttcgg
     1621 gataattttt cttgagagga acaaacacac ggatatgttc tttttccact cccaatccca
     1681 gacaaatatc ttccaatttg ccataaccag tatagttctg cccacctgtc attgcagttg
     1741 tggcattatc cagaatgatt atcatcacat tggctttatc gtaaatgcaa tccaacagcc
     1801 ccgtaattcc tgaatgacaa aaagtgctat caccaataac ggcaacggca ggaaaaagtc
     1861 cactatcgga agctcctttt gccattgtaa tagaagcacc catatccacg caagaattga
     1921 tggaattata cggtggcata aagcccaaag tataacagcc gatatcgctg aaaacattgc
     1981 ctctaccata ttccgccatc acttcattca gggataaaaa agtatcggaa tgaggacaac
     2041 caacgcataa agcgggaggt ctgccaacta caatatcggg aacaggtcta cctattgtat
     2101 caggtaagga aagagcttta gctacaagat taggattaag ctctccggca cgaggtagat
     2161 agccatcaag acgtccatga ataggtttca atccgggaaa agcaagccct ttaagctgtt
     2221 cttccacgaa gggcatacct tcttctataa ccagcaattc gtcacaggtc tcatagagtt
     2281 gacggatttg tttttcgggc aaaggatact ggcatatctt tagcactgga taaggaattt
     2341 cttcaccgtg atatgcttcc cgcagatagt tataagctaa gccggtagtg ataataccta
     2401 acttggtatt tttcctttct gccgtatata cattatacgg ggaattttcg gattctgcca
     2461 gaaagtcctt ttgcagggat agatgatgtt gatacttttt cctggcaatg gcaggaagaa
     2521 gcataaattg aaaaatatct tccggctttt tcaattcctt ttgcggtaaa ggattgcgtc
     2581 tggtaactcc agaacgggaa tgcgccaaac gcgtagttaa gcgtaatagt atcggaatat
     2641 gatatttttc cgagagttca aaaccgtaaa aagccagatc ataacattcc tgttgattgc
     2701 ttggttccaa aacgggaatc atcgcaaaat gtccataata gcggctatcc tgttcattct
     2761 gcgagctgtg cattgaaggg tcgtccgcga cggcaataat taaaccaccg ctggctcctg
     2821 taatagccga attcattaaa gggtcagctg ctacattcaa cccaacatgt ttcatcgtta
     2881 ccatagctct tttgcctgaa gcgctcattc ccaaagcggt ttccagtgcg gtcttttcat
     2941 tggaactcca ggtgcggtga acattgtttt cttctgcctg tttagacctt tgagcgtatt
     3001 caatgatttc ggtggaggga gttcccggat aaccgtaaaa acctgatatt ccggcatcca
     3061 atgctccctg ggcaagggct tcgtcaccca acagcataag ttttggcatt ttatctcctt
     3121 tttattttat ttattttaga aatttagatt ttccagacgc cactgccaat tccccaaagc
     3181 ggttcctgga atattcattc ttgcctcatc tcccagtccc aatatatctt gataagggaa
     3241 aataacctgt tggcaactgg atgagagagc aatattgcgc ataatgagat gaatgttttc
     3301 agcattcaaa tgcgcctgac aatcggtaaa ctgatgtagt gcacaatatt cctgtaagtg
     3361 tttataggac ggagaattgg gagaaagtga ttcaaaccag ccacgcaggg tttgattatc
     3421 gtgagttccg gtataaagcc aacgctcggg agggtatttt ttcacatcgg gaacgccttc
     3481 ttcaaagcag aactgcaaaa caatcatccc cggaaaacca tattgctcac ggatccggca
     3541 aacatcatca ttgagaatgc ccaggtcttc ggcaataaaa cgctttatgc taaaccgttg
     3601 taacagagcg gaaaagaaat cttcaggtaa ggcacgcacc caggaaccct taatagcgtc
     3661 tttaggcaaa actggttgac cattagcgtc cagctcgggc tctacctgcc aaaaattcac
     3721 atagccgata aagtggtcta agcgtaaaat atccagatag ccaagtgctt gtttgattct
     3781 ctccataaaa agctgaaatc catcctgttt caatacatcc catcggtata aaggatttcc
     3841 ccagagctga cctttttccg caaaagcatc aggtggaact ccggcaaagt gtaaacgcct
     3901 tccttgttca tccaagtcaa agtattccgg gtgagcccaa acttcagcgc tatcgtaagc
     3961 aagataaaga ggcaaatccc caattaaaat tatgcccagt tcttgtaagc gttctttgaa
     4021 ccaggataat tgttctacca gcattgcttg agtagcggca cgcagattga aataagaact
     4081 attcatcaaa cggacttttt gatagagctt ttcactgtag tgacgatgtt cttcgttcca
     4141 gttataccag caggtgtcgt cataaaactt gcttaaggaa agaaaggtta agtatggttt
     4201 caggaacgga gcattttctt caatataatc gtcagtccta ttggcaagca tccagttggc
     4261 agttgcctga gcaagcattt tatccttcag gatatagacc tgttcatagc gagtaacatt
     4321 gctgcgggga atttttaccg cttcaagcat cagctgattg atatagccct gttgatagag
     4381 caattccgga ctgataaaat aaggatttaa agcaaaagcg gaaagcggat tatagggtga
     4441 attgccataa ccgcaatgat ttataggtaa tatctgccag taagtatacc cctctgcttt
     4501 caggtattcg gcaaaacgga cggcttcagg tccgaaatca ccaatcccga aatcggaagg
     4561 cagtgaactg atatgcaata agatacctgt tttgcgcatt aggcatccag aattttaagc
     4621 aaacaattgc aaagcagaat ttgcggttcg gcaacggtta atttaaattg ttggtctgtt
     4681 tccagcagga gggcaaaaat tttttccagc gcattaatat tatagtaacg ggataaatcc
     4741 agaaattctc ttcgctgatt aggatggaaa tccgtaatat acttgcttac gatttcatta
     4801 tcggtaatat gagctttttt caggagtaat attttccaga tattgaaata gaaacgcgta
     4861 agctgaaaaa ggatttgcaa gggtttccaa tcggcaaaaa gcatcttttc cattgcttct
     4921 attgcctgtt ttgcttgttt tcttcccaga gcacgataaa aatcaatcaa agtcccgctg
     4981 cgtgtagttc ccaggctttg cataatatct tcctcggtaa ttgttgttcg gtcacgggta
     5041 agcaaatcca atttggtaag ttcgttagcg gcattataat aatcaagttc aatgcggttg
     5101 agaaattctt caattgcctt atcggtcatt tttttattaa tatctttcaa tgctttatcc
     5161 aaccaggcac gaatagcgcc tccgtagggt ggtgggtcac aagttattaa tttagttcct
     5221 gccttaattt tcttccagtt ttcgtggcgg gcatccgctt tttcggttac aatagctaaa
     5281 ctttggattt ccgaaggcga agaaaaataa tcggctaagg tactcagttc tttggtttta
     5341 agtgattctg catttttaat gataataagc ttggcagcgg aaaaaatgga ataagtatcc
     5401 agaaggtctg ccagttcagg gcttttcact tcatcaccat agataataaa agtatccgct
     5461 ttttcctttt ttttcagttt ggctctgatg gtatccaaaa ctctatctac cagataggaa
     5521 tctgttccaa taagcaaata ggaagaacct aaacgcactt tatccgctgt aaaatcaagg
     5581 gcaaatatac ttgtttccat taaaaaccca agataattaa aacaatagag agcaagccaa
     5641 ttagccaaca gtaaacagca aagtatttca aacgactgac ttcaattaaa cggattaaaa
     5701 aggcaataac cagataaccg acaagaaagg aacagacaaa gccagctaaa tagtttatca
     5761 gcaattgcgg cttcaaatta gcaaatgctt tatattggga aagatttcct gccagaattg
     5821 ccggaatgct taataaaaaa gaatattgag ctgcatcctt gcgcttcatt cctgtagcca
     5881 aagaacaggc aatcgttgtt ccagaacggg aaatcccggg taaaatggca actccttgtc
     5941 ctataccgat taaaacagct tgcagaaagc caatattgca agcaggaata cctttatcgt
     6001 tgaaataatc ggaaacaaaa acaataactc cagtaacaag caaaaagata gctaccaaca
     6061 tcggcatatt aaaaacagct tccataaaat ccccaaacag gatatatatc actcccgttg
     6121 ccaaagtgga aataatcaga taataaacca gcgtgcgatt tttacgatgc tgttcccgat
     6181 taacagtatt tccccaggaa aaaagcgact taataagctc ccaaagagtt ttacgaaaaa
     6241 agaccaaaac ggcaagcagc gttcccaagt gcataaagag ttcaaaaaca atgttttcac
     6301 tgtcaccaat gccaaaaaaa tgttgcgcta aaaccaaatg ccccgaactg ctaaccggaa
     6361 taaattccgt taacccttgt aatataccca tcaggattac ctgaattaag ctcataaatt
     6421 aacctctaaa gtatttcagg gacattatat ttatctatat cggtaagggg aatcaaagtg
     6481 aaacctgcag ctttcagacg gttgataaag cgttttaagt aatcaagttt atcgtaatta
     6541 tgacaatggg taatggcaat cacattttga ttacgattgg aaagctctat tatctggttc
     6601 agtttagcat ccatagtgga ttgactgata ttgggagaat ccagaaaaag gtcattgcga
     6661 tatgatttca gatgattttt ttgagcaacc gaataggcaa cggaaacatt ggtggtacga
     6721 ctgtccagaa agaatttatt gtgttttttc aaaatagtca tcacggcatt catcacctct
     6781 tcatccgttg ttgccaaact gcccatatga ttgttcgctc cttcacaata aggcagttgt
     6841 tcaataaagc gggtcattat tttttcaatc tgccgctcat catattgaac caagatagga
     6901 ttctttcccg gatttacttc cggatagcca atgggctcca taggaatatg aataagagtt
     6961 aggcgtcctt gctcctttgc cagtttcatt gtttgcacgc tatattcttc ttcgggaaag
     7021 atggaaaagc agacctcctt atccagagcc agaaaaccgg taagcaaatc accgccaatt
     7081 gttccgaagt catctacaat gatggtaata gtccgttttt tctgtttgtt ccgataaaga
     7141 ttattatcat aaaagatatt tatctcatat tgtttggcgg tatcgggaaa actgaagagc
     7201 aatgtttgtt tacctgctga gtccattccc tttataaatt tagctccatt gcgttccagt
     7261 tcacctttga aaatcatatt agcatatacg aggtccattt ccgatttatc aatgggcaca
     7321 ttgaatatag ttaagttttc cgtattgcgt ttgcggaaat aggacttggg gatgccaagt
     7381 ttttcaatgg ttgtggtaat tgccgattct ggagtgtttt ttgtattgtc tatagtggtt
     7441 tcctgggctt ttttatcctt gggtaatatc tttgatgcgt ttggagaaat tgccttcact
     7501 tcttttttgg tttttttctt tggtttaacc ggtagttcgc tgtttttaga aaccgtttcg
     7561 gctttagaga gctcggaatt cttatccgcg ggctctttta tgattatcca tatcaagaaa
     7621 cagcaaatga atcctgtcag aataaaaatt cccggattac cgccttttct ccgacgctta
     7681 accttacgca gtttcttttt acgcactttg ggcatagctt gtcctcaata gatttttgaa
     7741 aggtatgaaa cgattaacta aatattttaa tacccagata aggggaaaga aaaatagatt
     7801 ggataccaaa aatgcaccgg taagcactaa cacattcaca aagatatctt ctatcataac
     7861 aaagggataa agcttgaatt taatagatat gccggtatat ttgtcaataa agccgattaa
     7921 cggcatcaga gcaaaacgca ggattaactg atagaaacgc acagcaaaga aaacgactag
     7981 caaaataagt aaaatataac ggaaaagaag gaatttattg ttccgtgccc aagaaagaaa
     8041 cgctttcaaa gctctaggat gaccatactg cttttgataa tagggagtta aaaccacatt
     8101 aaataaataa tgataaagaa taagaaacac ggtatagagt ttgaataaca aaagcagacc
     8161 cgattttaca gtacccttta tcccgtaaaa atacgccaga aaactggaaa tggcataaat
     8221 tattaaaccg attaacacca aagccatcac gctaacatta aattgaatta ccatatctaa
     8281 atattcgcta ttattgcagt tgtatagtaa cttgattttt atcagcataa aaacactgaa
     8341 cacaaagata attaaaaagg caagcaaggg caaataatgc atccgaaaac taagcaaact
     8401 ggtagcggaa gatagcaaac tgccactatc tatccaatac aaaagagtta tgggggcaac
     8461 gaaaacctga attccgttgt aatcgccaaa aagggcattt aagatactct ttatcacctt
     8521 agcttgccgt aagtgcatta tagtccttca cttttgtttt atgtatcctt ttctggaaat
     8581 tacagaattc tgtcaatata aataaggaag caagaagagt agagggcaaa gggaatgtaa
     8641 gttgtgaggt gtcctgaaaa tagcggacta atattaagga gaaagaaatg aacaaaaatt
     8701 ctaatgaaag gatgcaagtc cgctaaatgg aactcaatgt gcggtacaaa tactttattc
     8761 caatcttatt ggtgacaatg tttatggttg ggcaaaagat gtttcgccgg ctgtttgcgg
     8821 accgaattat tgttctgcca agttcactta caggcttgaa tgcaccgatt taagcggttt
     8881 acctgttgcc ggagtttcag tttataatag tcaatccgcc ggaggtattt ttataaatcc
     8941 agcgattact aatagtgcag ggattgctac cggggagctt tatccaatga aaacgcagat
     9001 ttgggtaaat aatcctatta ccaatgaaat agcttttaat actcagtttt ttgctgaacc
     9061 gggagctctt attccctttt ctgtgcaatt gcctgttagt gtggaagaaa atatcgtttc
     9121 tattcctgcg ggaaaactaa atctatatcc ttctgtatta aatcctcatt ggaacgaaag
     9181 tatccgaatt tcttatgata ccaagcttac aggcaaggca gaacttgttt tatacgattt
     9241 gaaaggacgc catcttgcgg aaaaagttta tgaccaaaat gaaatagaat ggcagctgcc
     9301 aaagctttcc agcggtattt attttgtgcg tcttacaaat caaggtaagg cattggggac
     9361 agctaaactt attatactta agtgaaatac gcttttctcc tgctgttatt agctgaaatt
     9421 atgatgcttt ccgctctcac ttttgaagcg gaagagctga atttctatct ggaaaataat
     9481 ttatgggaaa tagatggtct atttcatttt gccaattacg attcgctttc cacgcacaaa
     9541 ctaatctttt ttcctgttcc cagtgatagt ttatgcctac cgcctaaaat taaagccata
     9601 gccattcagg atagcaatgg agcggaagta actatgctaa atcaagcaaa gaagggtttc
     9661 acttttctcc tttctctgcc tgctctttct ttttgttctt tgcaccttga ttacactcaa
     9721 acccttaaaa gcaattatgc taaatatatt atcaccacta caaattcctg gggcagaccc
     9781 ttaccttacg ccaaatatac tttgcatccg ggcataaatg tgcaaatacg ctctcttcct
     9841 ttctccgtgc agaaacaaga gggcaggaat tatatctggg aattttttga ttttgctccc
     9901 gaaaaggaat ttgaggttac attccaaacc attcctgcag aagatcagaa ctaactatat
     9961 agaaacacat taagataact tttcgcactt agccaacact catatccttc cgatatgatt
    10021 cccatatcgt tcccatatcg cgatatggga aagatatggg atcttattga tctatataac
    10081 tttaaagaaa aggaaaaaag agcggaaaaa agtgttctat tttaattcac aatttttcaa
    10141 tttggaaacc agttaaaatg aggttgtaag tgagatagtt agtaaattgt ggttttctgg
    10201 aaggcgggtg aaaattgtga gtcaaaattt tctttgctga ataatcgtga atctgacgag
    10261 gtgcttacgc caccatcgct attcttgtgt cgccctgacg ggctttttgg caaagggcag
    10321 agagcaaagg gtatctcaac cctctaaact ttctcaaccc tctcaaccct ttaaactttc
    10381 tcaaccctct caaccttctc aaccataaat ctaccaagtt tccttgttaa attcattctt
    10441 cattccaaat ccgtaaaatc ttcatagacc tgttcattca agacctatta ttcaaatctg
    10501 cgggaaataa tccttgtcca atcttaaaaa cggtgtttca agccgatata gagattacgt
    10561 ccttttcccg gctgttcgga aaagacccat tcttttcggt tgaatagatt ggcaagctga
    10621 gcataggcag cggaattatc cttatggtat tcagcaccta aattgaggat tatagcttcg
    10681 gggaggtcgt gtccacgatg gtctttagta aaatagtgct gtaaaatatc cagattaaaa
    10741 taccagttgc catagccata ggcaaaccgc gagtccagat taaggagtgg acgatagggc
    10801 gctctcgtat aatcgctttc ggttaagtag gcaagttcca gttccaaacc ctgatgcata
    10861 ttgagtttat ccattttgaa aaatgcttct acagaagtta aattggagaa gacatcggta
    10921 tagcgaaggg tgggaatgcg ataattattg gtgcttacta aaacagggct atctatttcg
    10981 tagcgcaggt tattgttgat attcaatctg cctaaggaga aatttatttt ttgaggatag
    11041 agatattcaa gacctgcatt gaagttcagc ggtgtctttt tcagtttatg agcatcgctg
    11101 aaagaaatcc agggtgttgc ttccagaaag gaaagatagt cgtttccttc caaaagaggc
    11161 acattggata aacttaaaac accccagccg gttatgggat aacgatagag aaattccagg
    11221 gaaggaacaa tagtgtaagc atcagcaagt aaatgaattc caggtttaga aatgtccata
    11281 atatcaccat tatagacagg ggcaatttga attccgaaat caccggattt taccataaat
    11341 ttggttttcc aattcaggat ttcctcttcc agacaggaag cgaggtatac attcaggtag
    11401 ttacatttgt agggcttatt gaggtttttt tggtagatat aattgtaacc caattgagcg
    11461 gaaatatcct tgaaagaaac atttccaata gtcaagtcgg gtctgtgatg actaaaatta
    11521 aaatccaaaa cggtattatc aaaatcatcc gcttgcgagg aaacattctg aaaacggaaa
    11581 gaaagcggaa aatgggaaga gagctcggtt cccagaaaca ggttgttatg aacggaaagc
    11641 aattcggaaa ttggagaacg catatccaga gtgtaagtta atgcattcag gataaagcta
    11701 tcgggataat aactgataaa actattaagt ccgaaatcca tatttccttc aaactgcaga
    11761 taactgcgat gttgcatagc gggaatcggc ttgggctttt ccagtaaagg aactcccgga
    11821 ggaacgaaag caggtaaact gtcccccata ttgagctccg gtgaaaagag caaagagcgt
    11881 ttatagagaa aaggtttgaa ggaactttct ccactaactg ttaaatcggg aagttcctgg
    11941 gtttgagaaa acaatagtgt tagggttaaa gcgagggtaa gaattaacaa agtgcgtttc
    12001 attgatttac ctccaaaaaa acatccgtca gttccatttt tgctgcctca tcaagttcct
    12061 ctctatattt gaaaaataat gcctctgctt cggtttttgc accggattcc agcaaagaca
    12121 agacggaata ataaattgct ttactgcgaa tttccttata ttcagggaaa agtaaaatca
    12181 cctttttcaa ggttgagata gctgaaagat aggaatgccg atgataatca atcattccca
    12241 ttaccagaaa ggcattacaa tggtctaaag tggacaaact gttattgatg ataaattcaa
    12301 tgagagcttc tgcttcttca aagcgctgtg cattatagag ctgacgcata cggataacat
    12361 ttacttcggg atgattctta agataatttg agcccaaaga ccaaagcttg tcaaaatcct
    12421 tatagttatt ggcttcggaa cattccagaa gcttaaccca agtagcgcca tcgggattgt
    12481 ttttataggc aagcttgtat ttttccaaag cggaatcata gttaccggta atatattcca
    12541 attctgccca tttcagtagt gcttcgttat ttttgctttg agcatacaat tccgcaagca
    12601 gggtatccgc ttcagcatat tgattcagct caataagcgc cgtagccata agcaattcaa
    12661 tgtcttcgtg cttttgttcg ggaaattccg ttctgatttt ttcagcggta gcaatgaggt
    12721 ctgcccattt taattcatca gcatagacct tcattaggat ataattgagt tcaaatttga
    12781 tatattcgct gaagaaggta tcaggtagaa cgagaagatc atttaacagt tcgtctttgt
    12841 tattcacccg tttcaaagcc agctccaagc caactaaagc atcgtgaatt gtagccagct
    12901 gattttcagt aaactttgta gtattgcgga aacgggttaa aatatttatc cagtcgttta
    12961 ccgctcgttc gtaattgccc atattataat aggtattggc aatatcggta agcgcttcca
    13021 gaaaatgaga ggactcggga tatttatcta taaaattgtt gtatagttcc aaagccagat
    13081 gatagtcctt tgcagcataa gcagatttgg cagcaaggaa aagataggta tcaggacttt
    13141 ctttctcccg ggaaagtttt aggtataagg ttgaggcatc tttaaagcgt ttcatttgat
    13201 ataaacagag agctctgtaa ctctgtgcct caattttacg cacattatca gtggcaaagc
    13261 cctccgcaag aataaactgt tctaaagccg gagaataatt cttttttgcc agcataatat
    13321 tgcctttcaa aaggcaaata tcgtagttgg gaacaaggtt ttgcaaaaag ttctcgcttt
    13381 ggtcataatc gcctaaatag aaataaattt gagcaatgcg taaggcaatc acttcatttt
    13441 ccggattctt ttcatataaa taaagatatt ttttcagagc cagattgtag tttgccaaat
    13501 aaaaatccat ttccgccaga taatataacg cttcattgcc ataaaacgat tccggataga
    13561 gattgataag ttcctgaaag ttatttttag cttcagtata atccttttcc agaaaactga
    13621 gataaccgag atagaaatat gccttatctg ctttgtttcc tgatggatag cgactgagat
    13681 aacgattgaa ggcatctttt gctaagttac tattgccagc gaaataaaat gcttcagcaa
    13741 tgaggaaatc aatcctgcta ttcaattccc tgcttgtttc gtaatttctg gcttgtccta
    13801 actgccttat tgcttcggta taatttttct gttgaaagta aaaatagccc aaagtataat
    13861 aaagttcagc tttcatcagc tcattaggag tttgttttaa ataggtctgc aattgttcaa
    13921 tggcattctg cggatttttc ttgaacaata attttaatcg ttccagataa gcaggaacgg
    13981 caatttccgg ttgggtagaa tttactaatt cagccaaaag cgaatctgcc tggttttctt
    14041 taccatccct aattaaaacc agagcgcggt aataattaaa ctgaggagta ttattattta
    14101 aaccctccag caaacgggag atttggtcaa aatcggatgt gcgtaatgcg cgacggattc
    14161 tgatatccac tatttctgga acgaaggcaa tctcgtccga tttataggac tctacaaagt
    14221 tgtcaaaatc attataatat tcattttcta aaagatattc caaccagctt tgaatgtaat
    14281 ttataaagta tgtatcttta ggtgattgat ttttaagcaa agtaaatgcc tgatcatctt
    14341 ttcccaaagc aaggaaagta gaaaacaggg aaaagagaag agcttcatta tcgggaaatg
    14401 ctttcacggc tctcaggtat gccttttccg ctgaataata ttgaccgagc tgaaagtaaa
    14461 tatcccctcg caaactgttt gccagttcca agtaagtagg gtttccagtt tcggtttcca
    14521 gacgctgaag aagttttagt gcagaatcat actgctttag ttgagttaga caataagcat
    14581 aattcaggat aatttcactt cgtacattct ggttgagatt tgtttccaac aaagaattat
    14641 aaatatcaat cgcttgggaa aaggcatttg cttccatagc cagattgcct tcaataaact
    14701 tgatgtaagg tagataaacg gattcgggat aggtagcttt aaattgagta atttctttgg
    14761 tcaaattcag agtgtcacct acattgtata gttctacaat atagcggtaa gcataattct
    14821 cgctattagt tgcagccatc aatggcgcat tattttgggg cacaatgaat tctgcaaaac
    14881 agaagcaggt aagggttaag gtaagcagag taaaaagaaa gtatttcata aacttttaat
    14941 ctcacctatt tattttgttt taagggaatg gaaacaaaca cggtagttcc ttcattttct
    15001 ttacttttaa tccagatttt gccaccgtga agttctgcga tgcgtttgga ggtggaaagt
    15061 cccaaaccaa gtccgctact gcgatattct ataattccgg atttgtgagc atagatttcg
    15121 tttagttcgt agaatgtgcg aaatacattc tggagttgat attcaggaat tccgatgccg
    15181 ttatcctgaa catatagaac caacgcttct tttccgtcta ttttttcgct ttggaaagcg
    15241 gatttgcgag ctcctataat aacggtgcca aaatcgttgg taaaacggat agcattcaat
    15301 acaaggttgt aaatcatcaa atgaagggct tcccagttgg cttttatggg gggtaaatcc
    15361 ttctctattt ccaatttaat gaacattcta cgatttcggg aaaggatttg gacttcctgg
    15421 tgaacgattt ccagaatttc ctgaaccgag atatcggtaa ggggcagtga tttggttaag
    15481 ttataagtat ttaaagtagt tatatcattg atagtaagga taagcttttt tacggaattt
    15541 tcaattttat taatgatatc ccttttttct tcctcatcgg caaagagatt gcgtttgaga
    15601 cgggtaacat aaccctgcaa agtagtgagc ggagtattca gttcgtgaga aacgatagcc
    15661 ataaattcat ctttcagcgt ttcaaaagta ttcagttcat tgttcttttg gagcagattt
    15721 tcatagagca tagcattttt taaagcgatg gtaatctgtt ccagaaggag cttaattaac
    15781 tctgtgttca aatacatttc cctagtatta tcattcaggt tatccaaata aatatagcca
    15841 taaacattgt tatctacaac aagaggagcg caaaaaatgc tgtgcaaaac ataatcctga
    15901 acgctgatgg cacttttgaa acggttatct tccactgcat tgtaggtgga aacaatctct
    15961 tgggtgtttt gggataagct taaaacggtt ttgctgacac cagaaactgt tggcaaaatt
    16021 tgtttggcgg cattgagctg aactttatag ataattgctc cgtcgctatc gcgtttgatg
    16081 agaaaacccc tcgtgctatt ggtaaagtca atacaggcag aaacgatggc gggttccagt
    16141 tcgttcagat tcacaattct catcaattcg tgagtaattg acatcagctg attcattttt
    16201 tctatgcgct gagtaatctg actgaagtcc tcaattctaa ccagcagagc agagagatgc
    16261 tgttttgtgg aacgcataag atagatttcc tgtttggtaa aaggcatttc acctttatcg
    16321 gaaacgagca agaaacctat ttttttagag ccaatctgaa agggaacaat catcagatga
    16381 cgattatttt gttccagaac gaccaggtta tcggttttat atgctctttc aatgaaaggc
    16441 ataagctggg tgttaattaa attgtctttt cccgtattgt aattttgaaa acatgtgtaa
    16501 gatgaaacct tttcggaata ttgcatcagc cagaattgcc agggtgccaa aacctgaata
    16561 agcccttttt caattaagaa ttttattctc tctatattat ggatattagt aatgttgtaa
    16621 agcatatcat tccattgttt aactaaatcc cgactgcggg aaacgacagg taaaaagtta
    16681 aagtttttca gatttttcaa gaaatagagg tttatggtta agtagttctg tctgccatca
    16741 ggactgatgt tttcagtgat tttttccaaa tatgctttgt aattttcaaa ttctgcttgt
    16801 gccaattcct cctgttttaa ttccgtaaat atctgtattt taatttggta gagttccaca
    16861 ttcagctgat aatattcata ttcctgccag gcaataaggt attcatttac ttgccttaaa
    16921 attactctga ggggaatatc gggattcaaa aggtcaagct ttaaagccaa aacttccagt
    16981 tcgcattgcc aatagcgata ttgattattt ttgatgacag gcagagcaat ttcaattaac
    17041 tcgcgcgcaa gttctacatc ctttaagccg taataacaac ggatttgtaa aacatagaaa
    17101 accgcgatag catagttatt atttatttct cctgcatgac gcagagcaat tttcagttca
    17161 ttcaaagctc tttcatagtc cttttccacc atagctgtta aagccagagc gttatgatag
    17221 aattcttctt cgtggatatg cttgtaattg atgtgaacat ttttggttag taatcttctc
    17281 agttttttag cattacctgt ttcgtaaaga tagtaaaaat aggtcttcac cagaggactc
    17341 atttccttaa tctggccatt aagcagttcc ggttcatttt cccaaataaa ggtgtaataa
    17401 tgcccaaaac ccttaatctt gcttttagct aaggcaagat tgcgttttac tgactggaga
    17461 taattgtcac tgcctttgga gaggactaat tcctggcatt ttaaaagttt ctgttcggca
    17521 gtagaaaaat cgcccatttt gatttttgct tcaccctgat ttaataaagc caatgctttg
    17581 gacattgtca aattgaggtc atcggaatat tgataagcca gttcggaata atgttcggca
    17641 attacggtta atccctgttt tagatataag tcgctaagat tattgtaaat caaacccaaa
    17701 tagcgtttta tattatatct tttccagata tttaaagctg taacgagatg ttcatccgct
    17761 tcttcaatct ttttctgagt actatagaat acccctaagt cattatgcat agcggctaag
    17821 cgaatcataa ccttggtatt attctcggtg ggaagctttt ccagaaagct ctctatggtc
    17881 tttattgctg catcaaagtc cttattgagc gaataatata ctgccaaacg gtcaacaaaa
    17941 gcaatttgca attccaaagt catctcttgt tttagggctt tatctatata gtgtttcatt
    18001 ttagaggggt caagcttggt ataaatttga gcataagcca accaaacaag tagctgatgt
    18061 tttcccgtgt gcacatattt ttcggctgtc tggaaatatt ttaatgcttg acgcaggttt
    18121 tcagcgagga aatcaaccgt tcctaaaagg taatgtttct caaagaaatc ggggagttca
    18181 gatattctct gcaagatgaa accggcacgc tcaaagaaac cggtcatttc cattttttcc
    18241 tgaaaaatag cgatatcagc aataatattt tggagaggaa cttgcagagc gggattaaaa
    18301 tccagattca aaacattaag aatggcttca aatgcttgtt cttgttcatt ttcctgattc
    18361 aacatctcat aatatttcaa atagtaaaaa cgggcacagc tataatcatt tgccagaaaa
    18421 gcattagcaa tcaaaccctt acaagtttca aaatccaaaa tcgtttcgtt agcgtaaaat
    18481 tttaaaacac gctgagaaac gagaatttgc cttctctcgt tgcattcgcc gaaaagtcgc
    18541 tgttttgctt caataaaagt gaagtaataa tatttccggt gttttcttaa aatttcgtta
    18601 taaatgctgt cgttgagcaa agaatataat tctgcatcct ttattttcag gatataaagc
    18661 atcaattcac gggttaaggg ggtttgaact atgcttaatt tctgcaaatg taaatagtta
    18721 acatctctta agtgacttaa gcgtgaataa atagaatgta acagccgagt tggcaattga
    18781 taatcactaa ggtttaaggg ataatgcaat tctttattat aatagagttt tttccgctgg
    18841 gtcaggtcaa ttaaaatttc tactataaat tggggatttc cagctgaacg tttgtatatt
    18901 tcctctataa aagcgttagg aacgccattc tgattactca cgggagttat atttgccaat
    18961 aaacgattga tatatttttt ggtttcttcc aaattcaagt taggaatgtg cagcataaca
    19021 gtatgttcaa tctggttgat tttattgtat tcgttacagg taatggcaat taaaatgcga
    19081 tgtttaacga tgtaagagga cataaaatta aggaaatcta tagtattatg atgcacatat
    19141 tggaaattgc gaataataaa gatgatggat ttgttgctgg aaagttcaat cagtaaactt
    19201 ttaacggatt caaaatctaa ttttaattca ctagtagttt ggctgattgt ttttgcttcc
    19261 tgttccgatt caaaaaggta acgacggaat ttctcggaaa tcatttgtaa atctgtatat
    19321 tcttgaattt catcggcact tagagattga acgaattctt tgatgagagc aaaaaatgcc
    19381 tcgtggtctg ttttggagca ggtgtaatca aaaataaagt atttcccgct gagcaaatga
    19441 taacggaaaa gggacataat actgtccttc cccaaaccat ctccacccat aacggatata
    19501 attttaccgt tgccattttc tactgctgga atataatcca acagttgatg tgcatatttt
    19561 tcccgcacca gataactatt gaaacgcaaa gtattgatta gagaccagga gacggaaaaa
    19621 ggatattgca ccggttgaat gcggtttatg taatctacca tttcttcact gtttgtaaaa
    19681 cggttatcgg gatttcggtc aagtaaccgc aaaataaact tttccagagg taagggaata
    19741 tccttgttca gctcgctggg aaaattggga atgaaatatt gattgccacc ggtaattaac
    19801 gcatttattt ggtctacact gaaaggaaaa ctgcctgtgg taagtttgta taaaataact
    19861 cccaacgcat agaaatcact cgctttgctg ggggactttc ccaagtaaac ttccggcgct
    19921 agataaggca atgaaccgga aacagcttgt gaatctttgg gagtatcaat tttagagaaa
    19981 ccatagtcaa tcagttttac aacaatggaa ttgccttcca gacggtaaag aacattttcc
    20041 aatttgaggt ctttatgtaa aatgttctgg gtatgaagtg cattcaaagc ataagaaatt
    20101 tgaacggcta tatcgtaaat cagatttatc ctggatttgc tgaatttgaa acgagaaagt
    20161 ggtttgccgt caaagtgttc actgataaaa taaatatgat cagctatatg accaaaatcc
    20221 actacccgat tcaaattggg atgctcaagt tgagtaatgc gatgcatatc ttccgcactg
    20281 aaacgagaat ataattcctc ggaagaaaca tactgaaaca gctttagcgt aaaaagcttg
    20341 tcagtacgaa tatcacgaac tttaaacaca ttagcccaag ttcctgaacc caagcgttct
    20401 aaaacttcat aacgatggtc tataaacata ttaaagctcc attgcctgat ttattggttt
    20461 tacgatgtat tttcaggcaa gcatattgta ggaatgtttc catctcaagt gcacccaaaa
    20521 ccactgttcc ggatgtttgt gaatttgatt ttccaatatt ttatccacct cttccagaac
    20581 aattttataa ttctcttccg tatcttccag ttcttcatgc aaaatcggag gaaagaactc
    20641 aaattgaatt ctgtcatctt ccgctctgcg tgcaaaactg ggaactatag gaatcttgta
    20701 acgcaaagaa agtttggcta ctcctttcca atgtgaagca ggaaaaccta aaaaatccat
    20761 taccaatcct gaactacccg cattttggtc aataagaatc gcaactatcc tattttcctt
    20821 aaattcgtga ataatgtctc gcaaaccgcg tttcatattg ataattcgca ttccgcaacg
    20881 ctcgcggatg gcattggtgt aattatcaaa tagagtattg cgttggggtt tggctattcc
    20941 gctgatggga attcctgctt taggcaaaat tcttgctgct tcccagtttc cataatgcgc
    21001 tgcggcaaag atcacacccc tatttagaga taatgcattg tcaatatatt ctctgccttg
    21061 aattcggatg tttttatata gcgtgtcgtc atccatcaga taactttcat aaacggttaa
    21121 cgccatattc cgatataatt ttttaacaat ttctgccttt tcctttttgc ttttatccgg
    21181 ataaaccttg ctaagctgtt ttaaggcaac atatttacgg atgccaatca cccaaccacc
    21241 aaaccagaag agacctttga gcactgactt tgccgctttc gctggaagaa gttttagccc
    21301 cgcaagagct atcctgaaac ctaagtattc tattttattt tgtatctgct tattaggcac
    21361 ttggctatcc ttaataaatc cacctttttc tcctgttacc attattttta acgggcttga
    21421 actgacaaga aatatctgaa agcggaggtg gatttttaca aagagagaaa gaaggaagta
    21481 aagggttggg agggttgaga ggacctttgc tcttttctct ttgctaaaaa aatatttcgg
    21541 tgttctaaat gctttcagtg ttttcggtgt tatgaataaa aaatccaggt aaccgatttt
    21601 aattgacaaa aatttggcat tctttaaatg tcacaaaaag tgataacact ggagatacat
    21661 tatgaacgac cagttgcaag atttactaaa acgggtttat gaagaaggtg ttgccaaagc
    21721 caatgcggaa gctgagaaaa taatcagtca ggcaaaggaa caagcggaag aaaccatagc
    21781 caaagccaaa gcggaggcag aagcaatttt gaaagatgcg gaaaaaaagg cagaagacct
    21841 gaaaaagaat actcaatccg atttgaatat ggcatataat catactctca cagctctcaa
    21901 gcaaaaaatt accgaccttg ttctttccga agctctcaat actaaattaa acgatgcctt
    21961 caatgatacg gaatttgtga aaaagcttat tctggaaacc cttacagctt ggagggaaaa
    22021 tacttcatcc ggaacaatta tcatcagtga aaagatgaaa ccccatttag acgagtttta
    22081 tattaaatcc ttaaaagata tctttgatgg taagttgaaa gttgattttt cacccgttat
    22141 gaaacagggc tttattatcg ctcccgaaga tggaacttat aaaattaaat ttgagggaga
    22201 tgattttgcc aacctgttca aaaattactt acgccctcgt actaaggaaa tcctctttgg
    22261 taatcaataa tttatgaaac aagaatatta ctatttcgtt tctggattgc ccaacatcaa
    22321 tattgatgat acaaaactgc cttggactcc ggaagaattt ttggcagagg caaagaccca
    22381 ggttagcgaa aatgattatc atcttctgga gattttacat ctgccgatgg atttggacaa
    22441 tctcctgaga gttttgtata aaacaaataa agaccgcaat atggaaggtc tttatgatga
    22501 tgctttctgg gaagaatatc tggctttttt acgcgatgta atagataatc cggaagttaa
    22561 aactcctatt gaattttcag ctctcccgaa ttttatcaaa gaaatagtgt taaatacctt
    22621 aaaacaggaa gaaatgcaac ctctaccaca aactgaattg gcacttctaa agaaatttta
    22681 tgcctggaca gcaaaaagtc cgaatgattt tatccgggac tggtttgcct ttgatgctca
    22741 tataagaaat attttaaccg ctataaacgg acgcaaattt aatctgcctt atgcccaata
    22801 tcttgtcgga gaaggtgaaa caacggaaaa actgagtaaa agccatactg cggatttcgg
    22861 tttgggcaaa gaagatgaac tttacaatgc actaactcgc atttacgaac aaaacaatat
    22921 tctctatcgg gaaaggggtt atgatatttt acgctggcgg tggattgatg attataactt
    22981 ctttaattat ttcaatatag accgcatttt aggttactat tgtcagttgc gtattttaac
    23041 tcgctggata aagtcatctc ccgaattggg aaaagaggtt tttaattcca ttttaagcga
    23101 tttgaacaat agtttcagct ttcccgctga tttcaatata aaaagcacgc aaagaaaata
    23161 gaaggtaaac aaatatgaca acaggtatag ttaaaggaat aatttccaac ctcgttcagg
    23221 ttgaggtttc aggtcctgtt tcccaaaatg aaatctgcta catcaatctg ggcaaagaaa
    23281 agttgatggc agaagtcatt aaggttgtag gaaacatagc caatactcag gtttttgaaa
    23341 gcacacgcgg tttaaaacca ggtgatactg ttgaatttac cgatcatttg ctggaggtaa
    23401 aacttggtcc cggaatgctt tccaagaatt ttgacggttt gcaaggtgac ctcaataaaa
    23461 gaaaaggtgt ttttttaact cggggtgaat atactgaccc cttagatgaa aattccctct
    23521 ggaaattcac tccccttgtc aaagttggag ataaagtaag tgcaggtgac tggttaggtt
    23581 cagttcccga aagttggatt actcataaaa ttatggttcc ttttgtgatg gaagatgaat
    23641 atactgttaa aagcatcgct ggggaaggtg actataaaat aaccgaaacc attgccgttt
    23701 tgatagacaa agaaggcaag gatatagaaa ttaatatgat tcaaaagtgg cctgtgaaaa
    23761 ttcccattac tgcttatgta gaaaaaccgc gtccctttaa gcttttggaa actggcgtcc
    23821 gcacaatgga tacactaaat cctatggtgg aaggtggaac cggttttatt ccaggtcctt
    23881 tcggagcagg aaaaacagtt ttacaacatt ccatatccaa aaatgcggaa gcggacttaa
    23941 ttattatggc tgcttgcggt gaaagagcaa atgaagttgt ggaactcttt gtggaatttc
    24001 ccgaactgga tgatcccaga acaggaagaa aacttatgga aagaactatc attgtttgta
    24061 acacttccaa tatgcctgtt gcggcaagag aagcatctgt ttatactgca atgactattg
    24121 cagaatatta tcgcaatatg gggctcaagg ttttactgct ggctgattcc acttcgcgtt
    24181 gggctcaggc attaagagaa atgagtaaca gaatggaaga acttcccggt ccggatgctt
    24241 atccaatgga ccttccagcc attatttcca atttttatgc ccgtgctgga tttgtttact
    24301 taaaaagtgg aactactggt tctataactt ttataggaac tgtttctcct gcgggtggaa
    24361 atttgaaaga gccggttacg gaatccacca aaaaagcagc acgttgtttt tacgcacttt
    24421 ctcaagccag agcagacagt aaacgctatc ctgcagttga tcctattgat tcctacagta
    24481 aatatctgga atatcccgaa gttattgaat atttgaattc caatgtaaat ccgcattggg
    24541 tgaaggatgt aaatcatacc aaagatattc tcttgcgcgg aaaagaagca caggaacaaa
    24601 taaatatttt aggtgatgat ggtgttccgc tttcgtatca tgacaaatac tggaaaagtg
    24661 aacttgtgga tagaattatt ttacaacaag atggctttga taaagtagac caatccaccc
    24721 cgatgaaaag acagcaatat atgctggaac taattttgga aatttgcgat acggatttcg
    24781 tgtttgaggg ctttgaagaa gttatgccct attatatgca gttaatcaat atctgtaagc
    24841 agatgaatta tatggaattt caaacgccgg aatttaacaa ttacgaagcg gaactacata
    24901 aaatagttgc agaaaggaga gctaaataat ggaaacccaa gcttttcaaa aaatatatac
    24961 caaactcaat caaataacta aagctacttg caccgttcag gcaacaggag tgggaaatga
    25021 cgaactggct acagttgcag gacgtttggc tcaggtagtt aaaattatag gtgataatgt
    25081 aactctgcag atttttgccg ggactgaagg aattggaact gatgctgaag ttgtcttttt
    25141 cggaaaagca cctactttga aagttagcga tgaattaggt ggcaggtttt tcaatgctta
    25201 tggtgaaccg attgacggag gtcctgaaat tgaaggtaaa gaggtggaaa ttggaggtcc
    25261 ttctgtaaat cctgtgcgca gaaaacaacc ttccgaattg attactacag gaattgcagg
    25321 aattgatttg aataatactt tggttacagg acaaaaaata cctttctttg cagaccctga
    25381 ccaaccctat aatgaagtaa tggctatggt tgccttaaga gctcagagtg ataaaattat
    25441 tttagggggt atggggcttt ccaacgacga ctatctgctt tataaaaaca cttttgaaaa
    25501 tgctggagtg atagatagaa tcgtttcttt tgtaaatact acggaagacc ctactgtgga
    25561 acgacttctg gttcctgata tggcgcttac tgctgcagaa tattttgctt tggaaaaaaa
    25621 ggaaaaggtc ttggtgttgt taactgatat gacactttac tgcgatgctt taagtattgt
    25681 ttccaaccgt atggaccaaa ttccttccaa ggatagtatg cccggttcgc tttacagtga
    25741 tttagcaaag ctctatgaaa aagcagtgca atttcccgat ggtggctcca taacaattat
    25801 tgcagttaca actctttcgg gaggagatat tactcatgcc attcccgata atacaggtta
    25861 cataacagaa ggacagcttt tcctgaaacg cgatacggat attggaaaag ttattgttga
    25921 tcctttccgt tctctttctc gtttgaaaca gcttgtgata ggcaaaaaga caagggaaga
    25981 ccatccccag gttatgaata ctgcagtgcg tctttatgcc gatgctgcca atgctaaaac
    26041 aaagctggaa aacggttttg acttatccga ttatgatatc cgcgtgcttg attttgccaa
    26101 agagtattcc gataaaattt tggctgttga tgttaatatc agcaccgatg aaatgttgga
    26161 tagaacctgg gaacttttcc agaagtattt caaccgtgct gaaataggta tcaaagacga
    26221 atttatgaat ttgtattgga agaaaggatg aacctgaaat ttcagtataa taaaatctct
    26281 cagttacaac tgattaaaca acttgcggtt aggcaaagag ctttgccaac cttgaaaaac
    26341 aaggaaagtg ccttacgcct ggaagttaaa aaagcgcgcg accaggcatt tgctttagac
    26401 caaaaggtga aagaacgtac tgcagaactt gatgctttta tgaaattgtt tgtggaattt
    26461 gatccttaca tcgtgaaaat taaaaatgtg gaaattaaaa cccgtaaaat agctggtgta
    26521 aaaacccccc tattggaaaa catagaattt gaaatagcgg actataacct ttttaaagct
    26581 ccttcctggt atcccgaagg aattgctttg ctgaaagaaa tttgccaact ccaaattgaa
    26641 agggactttt atattcgcaa gatgcaaatt ttggagcaag tgcggaaaaa gacaacgcag
    26701 aaagtgaatc tctacgaaaa agtacaaata cctgcctttg aagatgccat tctaaaaata
    26761 aaacgctatc tggaagatga agaaaattta tccaaggcat cacagaaaat acttaaatcc
    26821 cgttttgaac aggagagcca gctatgatag aaaagatgcg taaatatacc ttcgttctct
    26881 atcatcagga ttatgagaac tttttggcag agctgcaaaa gctgggtgtg gtgcatatta
    26941 ttcgtagctg tgatgaaaaa accgaatccc aaaccaaaaa ccttgaactg atgaaggaat
    27001 atggtgaatg tgttaaattc cttacaaaac taaaatccac tgcacctaag caggcaagtc
    27061 ctttacccac aaaggcattg ctgaataaaa taaatgaagc aagagaagaa aaggataaac
    27121 ttcaacactc tatagataca gttaaaaaac aaattcggga tttaagtccc tgggggcatt
    27181 ttgattacga tttggtgcgt aaacttaaag aatacggatt gaccattcgt ttctatacct
    27241 gcttaaaaaa tcactttaaa ccggaatggc aggaaaaata tcccttgaaa attattaacg
    27301 aagttggcgg aattctatac tttgtagtta ttacagataa tggagatccg gctttagaag
    27361 cggatacctt ttcttttcat cagcataccc ttcaggaatt tgaagacaag ctgaaggaac
    27421 tggaaaatga gcaaaaagat attgatgatt acttgtattc catagctcct acagctattg
    27481 aaatgtttca ggatgagctt aaacgcctaa gtcgggaata cgaatttgaa gatgctctcc
    27541 agcaaggtaa atatgaagcg gaaaatacta tccgtctttt aagcggttgg attcctgtca
    27601 gtttggaaca gggtttaaag gactttctgg agaagaataa cattatttat gtttcagaag
    27661 atgccaaacc tgaagaaaat ccccccataa aattaagaaa taactggttt gcccgtcttt
    27721 ttgagcccat cagcaaaatg tatatgttgc cctattacga tgaatttgat ttaactcctt
    27781 tctttgcacc tttctttgct ttgtttttcg gtttttgcaa tgc
//
LOCUS       DAOPFL010000010        27517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1716_length_27517_cov_92.411114, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000010 DAOPFL010000000
VERSION     DAOPFL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27517
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_27517_cov_92.411114"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..2018
                     /locus_tag="PLG69_01735"
     CDS             231..2018
                     /locus_tag="PLG69_01735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPI42262.1"
                     /translation="MLDQLRKKQKVVIYIVAIVFILGMAAVGIGELLSPKPYLGKVNG
                     TKITYEMYQKKIQEIYERYAEMYKDQPIDENTRRSIENQAWETLVNDILWQQQIKKHK
                     IKITEDDVLTEMQNNPPQELMQNESLQTNGRFDKSKYLSALKNNPQFFAAMEEYVRGY
                     LPRQKLQEKIKAQAGITIDSLKAQYAKETDSVSGKFIWFEPNKSDPVTVTDAEIKAYY
                     DKNRETEYKKGPASRIKYLAFEEKPSDKDYASVKLLADELYNRALKGEDFAALAKDNS
                     DDPGSKDNGGSLGVFGKGQMVPEFEQVAFALKVGEISKPVKTSFGWHIIRCDSIATIT
                     KDNPQIKASHILLKVEASDETKNAILDKAESAAKLIKKKGIDAAAKELGVEVQETDWV
                     PHDQDYIPGIGNNGALLQFMRKGKEKAISDVITDQQNRKIVAVLTDNKKVYYEDFEKV
                     KLRIKYQLEKEKKVANVKKKAEEFVAKYPSSEYFSAAAKEGWQIIELNNHKRGLSVPN
                     VGLCEEFTNAALALEAGQTSGLIHTKEGSFIISCTERIKPDFNAFAKDKDRQEQIRKT
                     LEDAAWNRWYDQMRKSAKIIDNRAKYGII"
     gene            2019..3422
                     /gene="glmM"
                     /locus_tag="PLG69_01740"
     CDS             2019..3422
                     /gene="glmM"
                     /locus_tag="PLG69_01740"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPI42263.1"
                     /translation="MNKLMTGVSGVRGVFADTLNPIIAIKYAAHFAILQKQSYPELKP
                     EIIVGRDSRTTGKAMLHSIISALISVGCDVTDLGIVSTPTLLLKVQESDAIGGIAITA
                     SHNPPEWNAMKFVDADGMFLSPEKAEKFLSSVEEDIPWAKWKAMGELKIDNTAIQEHI
                     AKILNIPYLNIEQIRSRQFKVVLDSVNGAGGLISPLLLKELGCTVYEINSEPNGIFAH
                     IAEPLKENLGQLEETVSFFKADIGFATDPDVDRLAIVSEEGKCIGEELSVALAELFVL
                     PKKKGDIVVNLSSSMLSDDIAKSFGVQVQRTKVGEINVGKKMQEINSPIGGEGNGGII
                     CPDVHYSRDAIAGMALILGLLAETNKPLSAIVNSLPKYYFAKDKITVDASLMETLMNN
                     APFFFQNYQLDTRDGIKAIGEKHFIHIRKSGTEPIIRIYVESDSPEKSRELCEIVKTM
                     LGEINTFKDTDNNERNM"
     gene            complement(3893..6055)
                     /locus_tag="PLG69_01745"
     CDS             complement(3893..6055)
                     /locus_tag="PLG69_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPI42264.1"
                     /translation="MNTVKKSLILLLFIFLLNSLTAEKLGYALSGGGARGFAHIGVLK
                     VLEEEGIHPDYIAGTSIGAIIGALYAMGYSATELEEMCLKLNWNELTRDTHQRKDLYI
                     GQKRWAPYGNAVFELNESWVPQLPSSVFVGNNLNLELFKLTAPASQIQNFNELPIPFI
                     CNATDLLTGEAVSFTEGSLLQALRASISIPSLFEPFELNGHYYIDGGVSQNMPIDLLH
                     QMGADKVLGIKVNSTLRDEERINNLLEILDQTINIGITRNLKEHLDECDLLIEPELTQ
                     FVSTDYQHLQDIIAAGEESARQMIDQIRAFKNEFYPSSPKITTFDKALNSFYITQINV
                     QGNKNISPSKVREYVGLETGKTYSSEEIANACINAWNSQLFKTIYPVLQKNQDNQYDL
                     FIYVKERERKQLAVNLVYNSEEKLMAGLVLSLNNYLLKNSRLLAELKLGGRNELNVDY
                     VKNFGEEWGAYYRLFPYVGERTIYEYSEHHKINSFKSTEFGINSGVGVFAKDLAIAEF
                     FVYTDQTNLHSDISQTPLLPESSVVSGFGIKGYHESLDDFIFPTSGARVFGKFNFARY
                     SEISDFIYSCFQGRAEFYVPVRNIFSVHSSIYYGSYLNSTEHDILDPFIISGTDGFMG
                     YSRYEVSAPHYQILSFGFSSLPKNRYFIDAGFQGMRYSEKDVWSNSEDWEYCGYLGLG
                     YKNSILPTKLYVSLNKDKKVHSFLSIGYDFDIFFFSRR"
     gene            complement(6172..6708)
                     /locus_tag="PLG69_01750"
     CDS             complement(6172..6708)
                     /locus_tag="PLG69_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42265.1"
                     /translation="MKMDFIFGNIFWGLLIILLGLSIFLKGFNLHIPLVRVFIAIIII
                     MFGIKLLFGIKTHKYNKPKHYSRESTINYSGSRNEYNIIFSSGIIDLTDLPADAKDVE
                     INVVFGSGTVILPDSIAFNINPTSVFGSTVLPPKRPELQGKPIDIESNVVFGRLEYKY
                     APPPKATTEHAETEKETF"
     gene            complement(6727..8937)
                     /gene="purL"
                     /locus_tag="PLG69_01755"
     CDS             complement(6727..8937)
                     /gene="purL"
                     /locus_tag="PLG69_01755"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPI42266.1"
                     /translation="MKKDEITPELVAEHNLSPEEYERILDILKREPTFVELGIFSVMW
                     SEHASYKNSALLLKTLPKEGSFILAKAGEENAGLIDIGQGLAISFKIESHNHPSALEP
                     YHGAATGVGGILRDIFTMGAKPIAVLDSLRFGKPENPKVQHLLKEVVRGIADYGNCFG
                     VPTVGGEVYFEDSYEGNPLVNAMAVGIVKHNKIARSAASGIGNAVLIVGAKTGRDGIH
                     GATFASVDLSEKSEEKRTAVQVGDPFLEKLLLEATLEAIEEGLIVAIQDMGAAGLTCS
                     SSEMAGKGNVGMEIDVSLVPQRETGMTPYEIMLSESQERMLVIVKPENLERIKSIFAK
                     WDLDAVQIGKVTADKILRVFYGEEKVAEIPAETLILGGSAPVYKRASKYPEYLETLYN
                     FDPDKVPEPSNYAEVLFKLISSPNLCSRFPVYNQYDYMVQIGTVVEPGSDAAVIRILD
                     TDMAIALSTDCNGRYCFLDPYSGTVSAVAESARNVACSGAKPIAITNCLNFGNPYKPE
                     VYYCFSETIRGMSDACKILGTPVTGGNVSFYNENPEGAIYPTPVIGMLGLMNDFRKAT
                     NQYFKKENTRIILLGSLQADLGGSEYLKIQFNEVAGKPPKIDLAEELRLQDLILELID
                     NSLINSAHDVSDGGLLVAVLECCFAPDNIFGATLNFAFSDKPSKVYFGEAGGRILISA
                     PPENCQAIKETAEKHNIEYHELGYVSGKAEFVVNNHIDEDIEILYSAWRQGIKI"
     gene            complement(8949..10049)
                     /locus_tag="PLG69_01760"
     CDS             complement(8949..10049)
                     /locus_tag="PLG69_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPI42267.1"
                     /translation="MLVLKDPGLRKLEKFIANNPLTHSTILHWTGSMWGIGCRLSSSE
                     TIARIETQKQRADKQGFIALIPDMSVLEQDKIPAPIRPLMNQYWPGNLTLVFNYDDPQ
                     FAGIKNGNKVALRVPSDPALRALISLLGEPLISTSVNVSGLMPEENYERIVRNYDSWF
                     DFALLPSSREMKRPSEPSTIIEYIGSEECGSNDDIKCIREGSIPFYEIKQAFQLPVVM
                     FVCSANICRSPMAEKLFRKMIREHNLGLATDSCGLLPGGRMISLESLQLLLERGIIDA
                     EQHISKQVTPQLISSSWLILTMEERQRDLLRSAYPNNARRIWTLNEIVDEQGDIDDPF
                     GNNLESYKKTCEIIEDRLQRLTEKLKNNELRI"
     gene            complement(10063..11160)
                     /locus_tag="PLG69_01765"
     CDS             complement(10063..11160)
                     /locus_tag="PLG69_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPI42268.1"
                     /translation="MPRKCLIILLCAFFTLSYADELEDKMRQLREMQRQLESTEQKVK
                     QTQTKKQQTESEIKRTASLKQRTEKNLQKLKTEEKIVQDSLKSVQQRIAVTEMRLQDM
                     HREQNAELDLLLRVDKSYKKLNINHRDQRLLKDFIQGTRQKIDILSGYKIALTQVQEL
                     HKAHATKVSRGMREESSKKSSYEKQIKTLSSQSQKLTEEQKRLQAQVEKLKKDSAELE
                     GLIAQLMKQQGKEPPSYQFTGKKISWPLRGKIIRSFGQETRSYGTSVVSNGIDIAAKE
                     GTKVVAADDGEVIFADRYGGQGKLIIIDHKNGFFTLYAYNSELLVSRGTKVKRGQTIA
                     KSGMTGSASEPSLHFELRKDGKAINPIPYLE"
     gene            complement(11221..12987)
                     /locus_tag="PLG69_01770"
     CDS             complement(11221..12987)
                     /locus_tag="PLG69_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42269.1"
                     /translation="MNKHIKWILEQYKAQKWFVLIMVVLTLLSSLVSIAFPYVFKEML
                     DLLKDILQFPQKYPEPMKAVYRIIKIMFAIGLAQLVTGLYPGIRGWVNMRFEHSLRVL
                     YFKHILTKDFSFFQKFRTGDIVTRLTDDLSDYPRVSWFLCSGIFRAFNSFSMIVFALV
                     VMFSINVKLTLLSIAPLPFMMLIFYFTADKVYTNYNRNQQAISAINNQLEMSFSGIRI
                     VKSFVSEEKYNRFFDLALAKRFKTEMSMVKLNAILSLIWQYIDYIAQIGVILFGGYLA
                     VKGEITVGTFFLFYTYLSMMIYPLIDLPQLFVSGKQAFVNIDRLEEMKNYPTLNDEFK
                     GHHKVKEFSTLSFENVSFQYPDKPSPVLKNVSFELGSGEKMLILGATGSGKTTIANLI
                     LGLLKANSGIIKINGKPINEIDIKSLRNIVAYVPQEPLLFGGSVKDNILFAVEQASEK
                     DYQIAVKTSQLEDEIASFPDRDNTFVGNRGLSVSGGQKQRLTIARALIKKPQLLILDD
                     ITASLDAENEEKLWQALRENYPDISAIVISHRLSTLHYVDSVLFIDSQGYAHKGSHDY
                     LVFNNQEYHDFLREHLKQENEK"
     gene            complement(12980..14737)
                     /locus_tag="PLG69_01775"
     CDS             complement(12980..14737)
                     /locus_tag="PLG69_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42270.1"
                     /translation="MAGDKQTFTYYLKLLFPFVKKDLGLLIFGLFAMLVTSGLRLLNP
                     LILAQIIDHSIPDKNMAQMFQYGAYFVIVVLLSGLLSYLQILLLSRLGIKIITKFKGN
                     VFSHLLKLPISWFNKQPVGELIARVESDSERVKVLFSDLSITIIGNVLFFIGGFVILF
                     ILQWQITIYILPAIILCGIGYWFLFKYLNKFFRLIRERYAIITAKITDYVQGIQMIQA
                     LNQETKALQDVEKASLDKKKVEVHTQIIEYGSQSIFSFMINVVFIIIVILISAPKIIA
                     GTLTVGILIVFIQYIYQLVWPLMQISENVMQIQRSFASLKRILELTELPTEDDTYTGT
                     KIPVFEQEIKFENVWFAYKEGEWVLKDISFTIPKGKKIALVGPSGSGKTTTVSLLCGF
                     YPVEKGKILIDGVPLSEMDFREWRKKIGLILQDVYLFPGSILENVRVYNDQISEDKVQ
                     KAISIVQLDDFIKKLPEGINAELAERGQNVSQGEKQLISFARALAFEAEIIIMDEATA
                     SIDPQTEAKIQRSMENLLSGKTALVVAHRLTSVLDADEILYFSDGRITARGKHNELLQ
                     TSPEYQKLVELQMLRVQDE"
     gene            complement(14786..14863)
                     /locus_tag="PLG69_01780"
     tRNA            complement(14786..14863)
                     /locus_tag="PLG69_01780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14826..14828),aa:Pro,seq:cgg)
     gene            15385..16299
                     /locus_tag="PLG69_01785"
     CDS             15385..16299
                     /locus_tag="PLG69_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPI42271.1"
                     /translation="MKIATTPRVLYYHIVADKFPEIYHSGISVSNFFRQLGQLKRRGY
                     KFRSLSEVLANPKKFNAKDIVLTFDDGFSTNYPVLMYLCKEYNIKPTVFLIGKCVDNK
                     EMAWNHKLLLMKRYCSPVKLSAQIDNYVPGATISSFFSRIEMSEKDTVTDSLWKEVMP
                     FSESDYLQKHKPFLSLAQMKSLQKAGVEFGIHSWSHPDFSRLIYPEAYTELDLCFKQM
                     EELSVPCKRYFAYPYGRPAQNEIETRLIKNFQLSATFGISYGLGDNSLPYTRWQRIKM
                     DGRKLTNREQFLLLPLLRSIKASRFNFL"
     gene            16406..18760
                     /locus_tag="PLG69_01790"
     CDS             16406..18760
                     /locus_tag="PLG69_01790"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPI42272.1"
                     /translation="MHNNIPFLWIIAPLGAILALIFAAIFFRQVKKQDPGNAKMQEIA
                     GYVREGAFAYLKQQYKGVGIFFIIAFSVFQIMAWGLGVLHWLIPWAFLTGGFFSGLAG
                     YIGMNMATLASNRTTQACTKGLNKGLRIAFRAGSVMGLTVVGLALIDISAWFFILNLL
                     KFPLQQITVIMLSFGMGASTQALFARLGGGIYTKAADVGADLVGKVEANIPEDDPRNP
                     ATIADNVGDNVGDVAGMGADLYESYSGSILATAALGMSAVTMLGPAYADWAMKFVVAP
                     MILAGLGILLSIIGIFTVSTKENAGTKELMFALNKSVYSSSILIAICAYFVTKALLPP
                     DYYFGVFISTVIGLAAGILIGLFTEFFTSHSYKPTREIANQADYGPATVILEGISVGM
                     RSTAAPVLIIVIGILSAFICSHGFSSIEMGLYGIGFGAVGMLATLGVTLAMDAFGPIA
                     DNAGGNAQMCNLPEDVRMMTDNLDSVGNTTAATGKGFAIGSAALTAMALLAAYLEEVR
                     GALHLVGTKAGKAVFLHTNYNTKFTENIPLAKASIADFIQFFQINVLNPKFLLGVFIG
                     AMVAFVFSSLTIKAVGRAAGKMVKEVRRQFKEIPGIMEGTGKPDYAKCVKISTQAAQR
                     EMLIPALVGLLSPIITGLIFGVPGVLGILAGALSTGFVLAVMMNNAGGAWDNAKKYIE
                     TEKKAKGTEIHKASIVGDTVGDPFKDTAGPCINILVKLMSMISIVFSCLIVSYCLHVE
                     KINPPFTKVHLDRKVENKVIMVDKDSLDVNGNYPPTTTTEIIVK"
     gene            complement(18920..20311)
                     /locus_tag="PLG69_01795"
     CDS             complement(18920..20311)
                     /locus_tag="PLG69_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HPI42273.1"
                     /translation="MNSLGFKFPVQERKTALYNLEEEWYLPLLSKHLGIPMQSIKRSN
                     FGQYSMAVNYLTSVLEEAAAINKVAVYNIDHMAQLLFYGPDVVTLLNRALTGNFTDMK
                     VGQCKYTLLLNEQGGVQDDMILMRVTDTSFILVINAGHDITDTIEVDGEKQEYLADID
                     RIMQCKKEGEEVWAKDISDTLLKIDVQGPLSYKLLKEVYGESVLKNRNNPEKNMNFFT
                     FNEFERNGHHYYLSRTGYTNRWGWELYIPVAVAEEDAKIIISKALDFGGLLVGLGGRD
                     ENRISAGPFGLPLMGQEYDPYHTPVNAPLFETAVDMNKEYFVGKEALAKEIAEGVQKR
                     LYIFVSEGIVSNRGIYKDGKRLGTVTSSINSPNVSWEMRLAIGSKRKNVNEEKGTAAI
                     GMGWFYSPLFEKDDEGKEIGEIDGKPIRIPVEFYREDENRKPTGTPVLGYVTLEGITP
                     ATAHKPLKNIENL"
     gene            complement(20567..21382)
                     /locus_tag="PLG69_01800"
     CDS             complement(20567..21382)
                     /locus_tag="PLG69_01800"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HPI42274.1"
                     /translation="MKQEIIELYNNPPEKWTAKHKALFAAFIDALNKGEIRSCEKEKS
                     IWKVNEWVKMGILLGFRMGELTIYQWSEAKPFFDKDTLPEKQFTLADRIRIVPGGSSA
                     RNGCYISAGVTIMPPAYINIGAYVDRGTLIDSHSLVGSCAQIGKNVHLSAGAIIGGVL
                     EPVGMRPVILEDDVFVGGNTGIYEGIIVQNKAVIASGTVITASTPIYDSVREKFLERD
                     SGNSFTIPSKAVVVPGSRRLKSNPDFQIACPIIIKYRDDKTDKAVELEQALRI"
     gene            complement(21379..22551)
                     /locus_tag="PLG69_01805"
     CDS             complement(21379..22551)
                     /locus_tag="PLG69_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPI42275.1"
                     /translation="MKQSRFDEIIDRRNSGCYKYDGLKMLYGKSELIPLWVADMDFAV
                     SDEIISALSERLKHPVFGYNLRLDDFYEAIISWQERRFDWKVQKNWIIAVPGLVPAIS
                     LAVLSLTEPGEGILIQTPVYRPFYDAVLDHKRKLLTSALVNTNGIYSIDWQDFEEKLK
                     QAKLFIFCSPHNPVSRVWTKEELTKIGSLCAKYKVPVFSDEIHADIVYPGYKHLPLAS
                     LNDFSEICLTGVSPSKSFNIAGLATAVVIVSNSEIKNKFNELNTKLHLYLGNTFGIRA
                     LIAAYSESENWLDELLCYLDKNRIYITEFVQNELHGVKISPIEGTFLAWLDFRNWGLT
                     EANLQEIIVNKAGLALEPGTEFGKDGEGFMRLNFGCPKSILEIALKRLQILAREYG"
     gene            complement(22532..23068)
                     /locus_tag="PLG69_01810"
     CDS             complement(22532..23068)
                     /locus_tag="PLG69_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42276.1"
                     /translation="MPREIKVKSIRNELPATYRYSPWTRFIMLFVALAICIYAIFFFV
                     KFVTGTTPMFFKVLPLIILFVALDSVFKQLTSLYSVTFYEDRICFGFLCKPKIIIPYE
                     NITSIDLTKQISFSIILKYKDKDREKIFKTSASFPKILEILLNLADFAPQAQLNDLLG
                     KVVSNLRNLAGKSETEQV"
     gene            complement(23047..23910)
                     /locus_tag="PLG69_01815"
     CDS             complement(23047..23910)
                     /locus_tag="PLG69_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPI42277.1"
                     /translation="MIVKEINDIQRVNLHLHTNVSDGNISPKELIKRSQEIGLDLISI
                     TDHDTADAYTQIPDNILPLRILPGMEISSLYKGDDVHILAYGCDFNNPALRAMTEMYL
                     KGRKERAQKIIKLLTDMGMPISLEEVIAIAGSRELIVRPHIAQVLVNHKFCQTRNEAF
                     EKYIGDNKPAYVPKPEVSVSDVISIIHQADGFAVIAHPGKLAKLSYLEDIITMDIDGL
                     EVWHPDHYQWEIENFIKIATEKGLYMTGGSDYHSEQDKHNLFDIIPLPEVVLTSVKKL
                     WKEYQCRVKLR"
     gene            23987..24766
                     /locus_tag="PLG69_01820"
     CDS             23987..24766
                     /locus_tag="PLG69_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPI42278.1"
                     /translation="MEYDPLKNRLARAIDMFPALRKLFYMAFNLIFLRQRYVLKEIRN
                     LFRETDKINLYDAGAGFCQYSAYVLSHWLNSNAFATDLKTDYLRSFSAYADIYYPGRF
                     TYKTTDLQAFIPSQKFNLALAIDIMEHIEDDVSALNNLYNALKKKGYLLLSTPSDLNE
                     TAKFTSEHIRAGYNKKELENKLNRIGFRIIKSIYTYGTYGALSWKLLIKIPLQLISKK
                     LYLLLPLYYLLVYPLAEILMRLDMKTDNKQGTGILIVAQKP"
     gene            complement(24935..27394)
                     /gene="sppA"
                     /locus_tag="PLG69_01825"
     CDS             complement(24935..27394)
                     /gene="sppA"
                     /locus_tag="PLG69_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPI42279.1"
                     /translation="MKSIVKALILLLPFLFCSLAMAQTKSDSIFVFLNKQDFPIASVD
                     NMFIPISNPSLLGTGSASGIGLAYLNDDKEWQNHYWIFLNTDLLSYIYERDHSYKYHT
                     LALGAELFPAHILPNLYAGTNYRWQEGGFEDGSFRTGVTYRPHNSTSLAFTWDNPKHQ
                     SPYYRLGLAVRPFVFTDAIADYRLELSVDANYARYEMDKDYEFKKPVLGIQTQILDGV
                     KVGATYNWEEESALVNFSLCPRNLEAGGLLRSKKNDNYGIAWAQLTDWEHKPFLGHTK
                     PSWYKMDLKGNIVTYTAPKYAIGKIKIYDTGDKSIESVIDNIKQAKDDPEIEGILLKN
                     PSFSTSLALQEELIDAFNDFKSSGKKVCFYYNNIGNGGYIFASSIADKIYLNPTGSVD
                     LRGLSISSPYLKNMLASLGIEVLNFRSHEYKDAGNMFSEERMTSAEREAYESLLQSLY
                     DQILQSMETGRGDRLVASADEIINGGPYFIANDALEKGLVDALIYEDKLNKQLKKDFK
                     FSSQYKELTEYREYAWAKPKENLVAVIYASGNIVSGKGTPGQKIAQETTVDLIRKARK
                     DKQYKGIILRVDSGGGSAQASDIILRELELAKTENKKPIVVSMGGTAASGGYYISCNA
                     DKIIAEPSTLTGSIGVVGLMFNGTEMFQKIKVNWDTVKKGEHADMGSIYRPWTEEEKQ
                     MVTRSIENCYDDFVKKVDEGRKNMTLEQVKQYAQGRVWTGEQAQKIGLVDELGGLEKA
                     KESMSELIDKKGKLTLVDATTKKEGLKISINIGELNVFTPVKVMNAVNSDYIKLYELW
                     SDFGQDKALMLCPVVPETLQF"
BASE COUNT         8535 a         5432 c         4891 g         8659 t
ORIGIN      
        1 tagtgcaatt tataatctct ggtagggggg tacttatacc ctttactagc caaatgtttg
       61 ccttttatta ccccaatgca catattcagt ttgatgagga catcaacctt caagatgaac
      121 ggcatccctg tcgttcagaa aacaagtatg aaaaaaatcc ttgttaaaaa atgaagcttc
      181 aaaaaggtgg cttttatatt agtaatttaa cctctataag gagttttaga atgctggatc
      241 agctgagaaa aaaacagaaa gtagttattt atattgtagc tatcgtattc attctgggta
      301 tggctgctgt tggaattgga gaattattat ccccgaaacc gtatctggga aaagtaaatg
      361 gcactaaaat aacctacgag atgtatcaga aaaagataca agaaatttat gagcgctatg
      421 ctgaaatgta taaagatcag cccatagacg aaaacacacg cagaagcatt gaaaatcagg
      481 catgggaaac attggtaaat gatattctct ggcagcagca gattaagaaa cacaaaatca
      541 aaattacgga agatgatgtc ttaaccgaaa tgcaaaataa cccgccacaa gaattgatgc
      601 aaaacgaatc cctgcaaacc aacggtagat ttgataaatc caaatatctt tctgctttga
      661 aaaacaatcc tcaattcttt gctgcaatgg aagaatatgt tcggggatat ctgccccggc
      721 aaaagctgca ggaaaaaatt aaagcccagg caggaatcac aatagacagc ttaaaagcac
      781 aatatgccaa agaaaccgat agcgtttccg gaaaatttat ctggtttgaa cccaataaat
      841 cggaccctgt aaccgttacc gatgcagaaa taaaagcata ttacgataaa aatagagaga
      901 ccgaatataa aaaaggtccc gcttcacgta ttaaatatct tgcctttgag gaaaagccct
      961 cagataaaga ttatgcctca gttaaacttt tagctgatga actttataac cgtgctctca
     1021 aaggtgagga ttttgctgct cttgctaaag ataattcaga tgacccgggt tctaaagata
     1081 atggcggttc tttaggtgtt tttggtaaag ggcaaatggt tccggaattt gaacaggtag
     1141 cgtttgcttt aaaggttgga gaaatcagca agcccgttaa aacaagtttc ggttggcata
     1201 ttatccgttg tgacagcatt gctacaatta ccaaagataa tcctcaaatc aaagcaagtc
     1261 atatactatt gaaagttgaa gcatccgatg aaaccaaaaa tgctatcctg gataaagctg
     1321 aatctgctgc caagttaatt aagaaaaaag gaattgatgc tgccgccaaa gaattgggag
     1381 ttgaagttca ggaaacggat tgggtgcctc atgatcagga ttatattccc ggtattggca
     1441 ataacggagc tttgttacaa tttatgcgta aaggtaaaga aaaagcaata tccgatgtaa
     1501 ttaccgatca acaaaaccgc aaaatcgttg ccgtgttaac agataacaaa aaggtctatt
     1561 atgaggattt tgaaaaagtt aagctacgaa ttaaatatca gctggaaaaa gaaaagaaag
     1621 tagccaatgt aaagaaaaaa gcagaagaat tcgttgccaa atacccttcc agcgaatatt
     1681 tctccgccgc tgccaaggag ggctggcaaa taattgaatt gaacaatcat aaaaggggtc
     1741 taagcgttcc taatgtagga ctttgcgaag aatttaccaa tgcagcttta gctttggaag
     1801 caggacaaac aagcggactt attcatacaa aagaaggaag tttcataatc agttgcacgg
     1861 aaagaattaa gcccgatttc aatgctttcg ccaaagataa ggatagacag gaacaaattc
     1921 gtaaaacatt ggaagatgct gcttggaacc gttggtatga ccaaatgcgc aagtcggcta
     1981 aaataattga taaccgcgca aaatacggta ttatataaat gaacaaatta atgaccggtg
     2041 ttagtggtgt taggggtgtc tttgcggaca cccttaatcc tattatagca ataaaatatg
     2101 ctgctcattt tgcaattctg cagaaacaaa gctatcctga actgaaaccg gagattattg
     2161 tgggaagaga tagtcgcaca acaggtaaag caatgttgca cagcataatt tctgccctaa
     2221 taagtgtagg ttgtgatgta acggatttgg gcattgtttc cactcctact ctcctattga
     2281 aagttcagga atcggatgcc attggaggca tagccatcac tgcatcacat aatccacctg
     2341 agtggaatgc aatgaaattt gtagatgcag atggaatgtt cttaagtcct gaaaaggcgg
     2401 aaaaattcct gtcctcggta gaagaagata ttccctgggc aaagtggaaa gcaatgggag
     2461 aattaaaaat agataatact gccatacaag aacatatagc caaaatttta aacattccct
     2521 atttaaacat tgaacaaatc cgttcccgtc aatttaaagt ggttctggat tccgtaaacg
     2581 gcgctggtgg tttgatttcc ccacttttac tgaaagaact tggctgcact gtttatgaaa
     2641 taaatagcga gcccaacggc atttttgccc acatagcaga acccttgaag gaaaatttag
     2701 gtcagttgga agaaactgtt agctttttta aagcggacat aggttttgct acagaccccg
     2761 atgttgaccg cctggcaatt gtttccgaag aaggaaaatg catcggagaa gaattgagcg
     2821 ttgccttagc cgagcttttt gtgcttccca aaaagaaggg agatattgta gtaaatctta
     2881 gcagttcaat gctttcggat gatattgcca aaagctttgg cgtgcaagtt cagcggacaa
     2941 aagtcggcga aataaatgtc ggaaaaaaaa tgcaggaaat aaattctcct atcgggggtg
     3001 agggaaatgg cggaattatt tgtcctgatg tgcattactc ccgtgatgct attgccggaa
     3061 tggctctgat tttaggtttg cttgcggaga caaataaacc gctttccgct atagttaata
     3121 gcttgccaaa gtattatttt gccaaggata aaataacggt agatgcctca ttgatggaaa
     3181 cattgatgaa taatgctcct tttttctttc aaaactatca actggatact cgggatggaa
     3241 ttaaagcaat tggagagaaa cacttcatcc atattcgcaa atcaggaacc gaacccatta
     3301 tccgtattta tgtggaaagc gattccccgg aaaaatcccg tgaactctgt gagatagtga
     3361 aaacaatgct tggagaaata aataccttca aagatacgga taataacgaa agaaatatgt
     3421 aagcggcttg agacaatctg tgataataaa aatcaggata aattccatag aaagacgaaa
     3481 ataatgtctt gattgccaga tagtaaacaa aattaagctg gcaatagata tcttacccga
     3541 tggtcccgtc gttcgtaaga ttatattaac cgcagcttta tcttaaatga aattacaaaa
     3601 cagaagcggt gctcccatca taattttgat ttataaaaat taccttgagg actttgtcta
     3661 actacctgtt gcatagtcat ttctttaacg acactttaac gatcctgtaa caatacccta
     3721 acttcgctaa ggaatcgtta aagaagtttt aaagaagcat aaaagattca acttggcgag
     3781 gaatagtgcg gagaaaaaat agacaaaagg caaagggttt atcagccatt tatcttaatt
     3841 gaaaaaatct gtgttatctt tattatctgt gttatttgtg agagctaaaa ccctacctgc
     3901 gggagaagaa aaagatatca aaatcatagc cgatacttaa gaaactatgc actttcttat
     3961 ccttattcaa ggaaacatat agtttggtag gtaaaataga atttttataa cccaatccta
     4021 aatatccgca atattcccaa tcttcagaat tactccaaac atccttttcg gaatagcgca
     4081 taccttgaaa tccggcatca ataaaatagc ggtttttagg caatgaagaa aacccaaatg
     4141 ataaaatttg atagtgggga gcactaactt catatctgga atatcccata aaaccatctg
     4201 tgccactgat tataaaagga tcaagtatat cgtgttccgt agaatttaaa taactgccat
     4261 aatagattga ggaatggaca ctgaaaatat ttctaacagg aacataaaac tctgccctac
     4321 cttgaaaaca gctataaata aaatcactaa tttcactata gcgagcaaaa ttgaactttc
     4381 cgaaaaccct ggctccggat gttggaaaaa taaaatcatc caaactttcg tgataaccct
     4441 ttataccaaa accgctaact accgaacttt caggtaataa tggggtttgg gaaatatcgc
     4501 tgtgaagatt tgtttgatcc gtataaacaa aaaattccgc aatagcaagg tctttagcaa
     4561 aaactcctac tcccgaattg ataccgaatt ccgtagattt aaagctattg attttatggt
     4621 gctcgctata ttcatagata gttctttcgc ctacataagg gaaaagacga taataggcac
     4681 cccactcttc accgaagttt ttcacataat caacatttaa ctcatttctt ccacccagtt
     4741 ttaattctgc caaaaggcgg gaatttttga gcagatagtt attcagagaa agaacaagtc
     4801 ctgccatcag cttttcttcg ctattgtaaa ctaagtttac agccagttgt ttacgttccc
     4861 gttctttcac atagataaag agatcatatt gattgtcttg atttttttgg agaaccggat
     4921 agatggtctt aaaaagttgt gaattccagg cattaataca agcattagca atttcttcac
     4981 tgctataggt cttccctgtt tccaaaccca catattctct tactttggaa ggactgatat
     5041 ttttatttcc ttgaacattg atttgagtta tataaaaact attcagcgcc ttgtcaaagg
     5101 tagttatttt cggactggaa ggataaaatt cattcttaaa agcccttatt tgatcaatca
     5161 tttgcctggc agattcctct ccggcagcaa taatatcctg cagatgttga taatcagtag
     5221 aaacaaattg agtaagttcc ggttcaatca gcaggtcaca ttcatccaaa tgttctttca
     5281 gattgcgagt tataccgata ttaatggtct ggtctaatat ttctaaaagg ttattaattc
     5341 tttcctcatc gcgcaaggta gaattaactt ttatacccag aaccttatcc gctcccattt
     5401 ggtgtaaaag gtcaataggc atattttggg aaacaccgcc atcaatgtaa tagtgaccat
     5461 tcagttcaaa gggctcaaac aaacttggaa tggatataga agcacgcaat gcctgaagta
     5521 aagacccttc tgtaaaagaa acagcttcgc cggttaataa atcggttgca ttacagataa
     5581 aaggaatggg cagttcatta aaattctgaa tctgagaagc gggtgcggtt aatttaaata
     5641 attccagatt aagattatta cctacaaaaa cggaagaagg aagttgtgga acccaggatt
     5701 cattaagttc aaaaacagca tttccataag gtgcccagcg tttttgtccg atataaaggt
     5761 cttttctttg atgtgtatcc cgcgtcaatt cattccaatt caatttcaga cacatttctt
     5821 ccaattcggt agcagaataa cccattgcat aaagagctcc aataatagct ccaatactgg
     5881 ttccagctat ataatctgga tgaatgcctt cttcttccaa aacctttaaa acaccaatat
     5941 gggcaaaacc tcttgctcca cctccactta aggcatagcc aagtttttcg gcagttaagc
     6001 tattcaaaag gaagatgaat aacagtagga taagtgactt cttcacagtg ttcataacaa
     6061 ttataagtta catattcctt tgtcctgcct gcgcaagtag gaatccataa atccacctaa
     6121 gaatgaacat aataactgca tttgttcaat gaaaaccata tcggttaggc attaaaatgt
     6181 ttctttttcc gtctcagcat gttcagttgt tgcttttggg ggtggtgcat atttatattc
     6241 caggcgtcca aaaaccacat ttgactctat atcaataggt tttccctgga gttcaggtct
     6301 tttgggcgga agaacagttg agccaaaaac agaagtagga ttaatattga aggcaatgct
     6361 atccggtagg ataactgttc ccgaaccgaa aaccacatta atctctacat cttttgcatc
     6421 ggcaggaaga tcagtgaggt caattatacc cgaggaaaag atgatattat attcgttgcg
     6481 agaaccgcta tagttaatag tgctttccct ggaataatgt tttggcttat tatatttatg
     6541 cgttttaata ccgaagagca gtttaatgcc aaacataatg ataataatgg caataaacac
     6601 tctcaccaaa ggaatatgaa ggttaaatcc tttcaaaaaa atggataatc ccaaaaggat
     6661 tatcagtaaa ccccaaaaga tattgccaaa aataaaatcc attttcattt ccttatcctt
     6721 tttattttat atttttattc cctgcctcca ggcagagtaa agaatttcta tatcttcatc
     6781 tatatggtta tttacaacaa attctgcctt gcctgaaaca taacccagtt cgtgatattc
     6841 tatgttatgt ttttcggcag tttcttttat ggcttggcag ttttccggag gcgcactaat
     6901 taaaattcta ccgcctgctt caccaaaata gaccttagag ggcttatccg aaaaggcaaa
     6961 attcagtgta gctccgaaaa tattatccgg agcaaaacag cactccaata cagctactaa
     7021 taatccacca tcggaaacat catgagcgct atttattaag ctattatcaa tcaattccaa
     7081 aattaaatcc tgcaagcgca gttcctctgc caaatcaatt ttgggaggtt tgccagcaac
     7141 ctcattaaat tgtattttaa gatattcact acctcccaag tccgcttgca aagaacccaa
     7201 aagaataatt cttgtgtttt cctttttgaa atactgattt gttgccttac gaaaatcatt
     7261 catcaaacca agcattccga taacaggtgt aggataaatt gctccttccg gattttcatt
     7321 atagaaagat acattacctc cagtaactgg agttcctaaa attttacagg catcgctcat
     7381 tccccgaatt gtctcactga aacaataata gacctccggc ttataaggat tgccaaaatt
     7441 aagacaattg gtgatggcaa ttggtttagc tccactgcaa gcaacatttc tggcggattc
     7501 tgcaacggca gaaactgttc ccgaataggg gtctaaaaaa caatatctgc cattacagtc
     7561 ggtgcttaag gcaattgcca tatccgtatc caaaattcta attactgcgg catccgaacc
     7621 tggttctaca acagttccaa tttgcaccat ataatcatat tgattataaa ccggaaagcg
     7681 actgcaaaga ttgggagaag aaataagctt aaaaagaact tctgcataat ttgagggttc
     7741 gggaacttta tccggatcaa aattatataa tgtttcaagg tattcagggt atttgcttgc
     7801 gcgtttataa accggtgccg agccacctaa aataagtgtt tcagctggaa tttccgctac
     7861 tttttcttct ccgtaaaaaa cccggagtat tttatcggcg gtaactttac caatttgaac
     7921 tgcatctaaa tcccatttgg caaatatgga cttgatgcgt tccagatttt ccggtttcac
     7981 aatcacgagc atcctctcct gcgattccga aagcatgatt tcataaggag tcattcctgt
     8041 ttctctttgc ggaacaagag aaacatcaat ttccatacct acatttcctt tccctgccat
     8101 ttctgaactg gaacaggtta aacccgcagc tcccatatct tgaatagcaa caataagccc
     8161 ttcctcaatt gcttctaaag ttgcctccag caacagtttt tccagaaaag ggtctccaac
     8221 ctgcacggct gttctttttt cttcggattt ttcactcaaa tccacagagg caaaagtagc
     8281 tccatgaatt ccatctcgtc ctgtttttgc gcctacaatg aggactgcat tcccaattcc
     8341 ggaagcagct gaacgggcta tcttattatg tttcacgatt cctacagcca tagcattaac
     8401 taaaggattg ccttcatagc tatcttcaaa atagacctct cctccaacgg tgggaactcc
     8461 gaaacaattt ccgtaatctg ctatgcctct aacaacttct ttcaatagat gctgaacttt
     8521 aggattttcc ggctttccaa aacgcaaaga atccagaaca gcaatgggtt tggcacccat
     8581 agtaaaaata tctcttaaaa tgcctcctac acctgtagca gcaccgtgat agggttccaa
     8641 agcagaagga tgattatggc tttctatttt aaaagatatt gctaagccct gtccaatatc
     8701 aatcaagccg gcattttctt ctccggcttt ggctaaaata aaggagccct ctttaggaag
     8761 ggtctttaat aataaagcgg agtttttata acttgcatgt tcactccaca ttacgctgaa
     8821 aattcccagt tctacaaaag tcggttcacg ttttagaata tctaaaatgc gttcatattc
     8881 ttcagggctt aagttatgct cagcaacaag ttcgggtgtt atttcatcct ttttcataaa
     8941 attaacttct atatcctcaa ttcattattt ttcagctttt ccgttaatct ctgcaaacgg
     9001 tcttcaataa tttcacaggt ttttttatag ctttccagat tatttccaaa aggatcatct
     9061 atatctccct gttcgtcaac tatttcattc agagtccaaa tcctgcgtgc attattggga
     9121 taggcactgc gcaataaatc acgctgtctt tcttccatcg ttaaaatcaa ccaactggag
     9181 gaaatcaact ggggagtaac ttgtttagaa atatgttgtt cagcatcaat aatgcctcgt
     9241 tccaacagca actgaagtga ttctaaggaa atcatccgtc ctccgggaag caaaccgcaa
     9301 gaatctgttg ccagtcctaa attgtgttct ctaatcattt tgcggaatag cttttccgcc
     9361 attggagaac gacaaatatt ggctgaacag acaaacatca ctaccggtag ttgaaacgcc
     9421 tgttttattt cataaaaagg aatagaaccc tctcgtatac attttatatc gtcattactt
     9481 ccgcattctt cagaaccgat atattctata atagtagaag gttcggatgg tcttttcatt
     9541 tcccgggaag aaggtagtaa agcaaaatca aaccaagaat cataattacg cacaattctc
     9601 tcataatttt cttccggcat cagacctgaa acattaacgc tggtactgat aaggggttct
     9661 cccagcaaag aaattaaagc tcttaaagca ggatcggaag gaactcttaa ggcaacttta
     9721 tttccatttt ttattccggc aaattgaggg tcatcatagt taaaaaccag ggttagattt
     9781 ccaggccaat actgattcat caaaggtcta ataggtgcag gaattttatc ctgttctaaa
     9841 acactcatat cgggaataag agcaataaaa ccctgtttat ccgctctttg tttttgtgtt
     9901 tctatgcgag caattgtttc tgaagaggaa agtcggcaac cgattcccca cattgaacca
     9961 gtccaatgca aaatagttga atgggttagc ggattattag caataaattt ttccagcttg
    10021 cgtaagccgg ggtctttcaa aacaagcatt atttcttcct cctcattcca aatacgggat
    10081 aggattgatc gcttttccat ctttacgcag ttcaaaatgt aatgagggtt cacttgccga
    10141 gccggtcatt ccgcttttgg caatggtttg acctcttttt actttagttc ctctgcttac
    10201 cagtaattca ctgttatagg cataaagagt gaaaaagccg tttttatgat caataataat
    10261 aagtttgcct tgtccaccgt agcgatcagc aaaaattact tctccatcat cagcagcaac
    10321 tacttttgtt ccttctttag ctgcaatatc aattccgtta ctaactacgc ttgttccata
    10381 gcttcgggtt tcctgtccga aactacgaat tatttttcct cttagtggcc aggagatttt
    10441 tttgccggta aattgatagc ttggtggttc tttgccttgt tgtttcatca attgagcaat
    10501 gagaccttcc agttcagcgc tgtccttttt cagtttttcc acctgtgcct gtaatctttt
    10561 ctgttcttca gttaattttt gactttgact ggaaagggtt ttaatctgtt tttcgtagct
    10621 ggatttttta gagctttctt cgcgcatacc acgagaaacc tttgtagcgt gagctttatg
    10681 aagttcctga acttgtgtaa gcgcaatttt atagccggaa agaatatcta ttttttgtct
    10741 ggtgccttga ataaaatcct tcagtaaacg ctgatcacga tgatttatat ttaatttctt
    10801 ataggattta tccactcgca aaagcaaatc cagttccgca ttttgttctc tgtgcatatc
    10861 ctgcaaacgc atttccgtaa ccgcaattct ttgctgaact gatttaagag aatcctgtac
    10921 aattttttct tccgttttca gcttttgcag gtttttttct gttctttgtt tcagagaagc
    10981 agttcgtttt atctcacttt cggtttgctg ttttttggtt tgtgtctgtt tcactttttg
    11041 ttctgtgctt tccaattggc gttgcatttc tctcaattgc cgcattttat cttccagttc
    11101 atcggcataa gatagtgtga aaaaagcaca tagcaaaatt attagacatt tacgaggcat
    11161 tatattctcc tctcaaaaat ctcactgcca gaatttccaa gtgtcctata agtgtcaatc
    11221 ctatttttcg ttttcctgtt ttagatgctc tctaagaaag tcgtgatatt cctgattatt
    11281 aaaaaccaaa taatcatgag aacctttatg agcatagccc tgagagtcaa taaaaagcac
    11341 actgtcaaca taatgcaggg tagaaagacg atgagaaatt acgatagcgg aaatatcagg
    11401 ataattttcc cgtagtgcct gccaaagttt ttcttcattt tctgcatcca aggaagcagt
    11461 tatatcatct aaaataagca gttgcggttt ttttattaag gcgcgggcaa tggttaatcg
    11521 ttgtttttga ccaccggaaa cgctaagtcc tctatttcct acaaaggtat tatctctatc
    11581 cggaaaagaa gcaatttcat cttccagctg agaggtttta actgcaattt ggtaatcctt
    11641 ttcagatgct tgttctaccg caaaaaggat gttatctttc accgaaccac caaaaagaag
    11701 tggctcctgg ggaacatagg caacaatatt acgcagggat ttaatatcta tttcatttat
    11761 gggctttcca ttgattttaa tgatgccgct attggctttc agcagtccta aaataagatt
    11821 ggcaatagtt gtttttccac tgcctgtagc tcctaaaata agcatttttt ctccgctgcc
    11881 aagttcaaaa ctaacatttt taagcacggg agagggctta tcgggatact ggaaagatac
    11941 attttcaaaa gataatgtgg agaattcttt taccttatga tgacccttaa attcgtcatt
    12001 caaggtagga taattcttca tttcctccaa acggtctata tttacaaatg cctgtttgcc
    12061 tgaaacaaag agctgaggca agtcaattaa cggataaatc atcatagaaa gataggtgta
    12121 aaacaggaaa aaagtgccca ccgtaatttc tcctttcacg gcaagatagc caccgaatag
    12181 gataactcca atttgagcaa tgtagtcaat atattgccag attaaagaaa gaatggcatt
    12241 tagtttcacc atactcattt ccgttttgaa ccttttggca agcgcaagat caaaaaagcg
    12301 gttatatttt tcttcgctca caaagctttt tacaatcctg attcccgaaa aactcatttc
    12361 cagttgatta ttgatagcag aaattgcctg ctgattacga ttgtaattcg tataaacctt
    12421 gtccgctgtg aaatagaata tcagcatcat aaatggcaga ggagctatag aaagaagggt
    12481 aagttttaca ttgatgctga acatcacaac caacgcaaaa acaatcatac tgaaagaatt
    12541 gaaagcccga aaaataccgg aacataaaaa ccagctaacc cttggataat cggaaaggtc
    12601 atctgttaaa cgggtaacta tatctcctgt tctgaatttc tggaagaaac tgaagtcctt
    12661 ggttaaaata tgcttaaaat aaagcacccg caaagaatgc tcaaaacgca tatttaccca
    12721 gcccctaatt ccgggataaa ggcctgtaac aagttgtgcc aaaccgatag caaacataat
    12781 tttaataatt ctataaaccg ctttcatcgg ttccggatat ttttgcggaa attgtaaaat
    12841 gtccttcagc aggtcaagca tttctttgaa aacataagga aaagcaatag acacgaggga
    12901 agacagaagg gttaaaacta ccattatgag cacaaaccat ttttgtgcct tatactgttc
    12961 cagaatccat tttatgtgtt tattcatcct gcaccctcaa catttgcaat tccaccagct
    13021 tttgatattc aggggaggtc tgtaacagct cgttatgttt acctctggca gttattctac
    13081 cgtcactgaa atataagatt tcatcggcat ccaaaacgga agtaagtcgg tgagctacaa
    13141 ccaaagctgt ttttccggaa agcagatttt ccattgagcg ttggattttt gcctcggttt
    13201 gagggtctat ggaagctgtt gcttcatcca taataattat ttccgcttca aaagccagag
    13261 ctctggcaaa actaatcagt tgtttttcac cctgggaaac attctgtccc cgttctgcca
    13321 attctgcatt aattccttca ggcagttttt ttataaaatc atccaattgg acaatagaaa
    13381 ttgctttttg caccttatct tcgctaatct ggtcgttgta aacgcgcaca ttttccaaaa
    13441 tgctaccagg gaaaagatac acatcctgca aaataagtcc aatttttttc cgccattccc
    13501 taaaatccat ttcgcttaaa ggaacgccat caattaaaat tttgcctttt tccacaggat
    13561 aaaaaccgca taacaaactt accgtagttg ttttaccact gccggaagga ccgactaacg
    13621 caattttttt gcctttggga atggtaaatg atatatcctt cagcacccat tcaccttctt
    13681 tataggcaaa ccacacattt tcaaacttaa tttcctgttc aaaaacaggt attttcgttc
    13741 ctgtataggt atcatcttct gttggcaatt ctgttaattc caatattctt tttaaggaag
    13801 caaaagaacg ctgaatctgc attacatttt ccgaaatttg catcaggggc caaacaagct
    13861 gataaatata ctgaataaaa acaatcagaa ttcctaccgt taaagtccca gctataattt
    13921 taggtgcaga aatcagaatt actataatga taaagaccac attgatcata aaactgaaga
    13981 tactttgaga accgtattca attatctgcg tatgaacttc tacctttttc ttatctaatg
    14041 atgccttttc cacatcttgt aaagcttttg tttcctgatt taatgcttga atcatttgaa
    14101 taccttgcac ataatccgtt attttagcgg taattatggc ataacgttcc cgaatcagac
    14161 gaaagaattt attcagatat ttaaacaaaa accagtagcc aattccacaa agaataattg
    14221 ccggcaaaat atatatcgta atttgccatt gaagaatgaa gagaattaca aaaccaccaa
    14281 tgaaaaacaa tacattgcca attatagtaa tagacaaatc cgaaaacaga acttttactc
    14341 tttcactatc actttccacc cgtgcaataa gttcccctac gggctgttta ttgaaccagg
    14401 aaataggtaa cttcaataaa tgactgaaca cattaccttt gaatttagtg atgattttta
    14461 tgcctaaacg cgataataac aaaatctgca aataggaaag aagaccggaa agcaaaacaa
    14521 ctattacaaa atatgcgcca tactgaaaca tctgcgccat attcttatcc ggaatactgt
    14581 ggtcaataat ttgtgctaaa ataagtggat tgagcaagcg taagccggaa gttacaagca
    14641 ttgcaaaaag tccaaagatt agcaagccca aatccttctt cacaaaagga aagaggagct
    14701 tcaaataata tgtgaatgtc tgcttgtctc cagccacttt ttacaccttt tttctgcttc
    14761 tttttatagt gatttataca aaaaatggtc gggatgagag gatttgaacc tccgacttct
    14821 cggtcccgaa ccgagcgcgc taaccggtct gcgctacatc ccgatcttta atcagcacta
    14881 aatttgttat tgtctttttc tgtcaagcaa gaacttttgc ctcaatcatt aaaaacctgc
    14941 cttgtttatg ttatttgcaa catacagtat tgcaatatac tacagaaatt ttttcctttt
    15001 tccctatttt tatcccctgc atattttggg cttaacttta tatctatttt tattccaagc
    15061 tgttataccg ttttttcttg ccttccttaa tagcattact tacccattgc ttacccattg
    15121 ctaatggcat tagtattggg taagcattgc ttctgtaatg caaataagaa acctcagtca
    15181 aagcaagtca gtaaaagcga ggagtcgtta ttatgtaagt cagaaatcat catccggatt
    15241 tataaaataa aaggatttcc gtgctataaa gggcacaatt tctttcttta aactcggcat
    15301 tgggattttc cggtaaaaaa tgttccttga cagttatgta ttgtttatta aaaggaaaaa
    15361 attaaaagta actggattaa atatatgaag atagctacta ctccccgagt tctttactac
    15421 catattgtag ccgataaatt cccggaaatt tatcatagtg gtattagtgt ctccaatttt
    15481 tttaggcaat tgggacaact aaaacgccgg ggatataaat ttcggtcgtt aagtgaagtt
    15541 ttggcaaatc caaaaaaatt taacgctaaa gacatagttc taacttttga tgatggtttt
    15601 tcaactaact atcctgttct tatgtattta tgtaaagagt ataatattaa gccgactgtt
    15661 tttttaattg gtaaatgcgt tgataacaaa gaaatggcat ggaatcataa attgctttta
    15721 atgaaacgct attgttcgcc ggtaaaactt tcagcgcaaa tagacaatta tgttcccgga
    15781 gcaacaatat catctttttt ttcccgcatt gaaatgtctg aaaaagatac cgttaccgat
    15841 tccctttgga aggaagttat gcctttttca gaaagcgatt atctccaaaa acataaaccc
    15901 tttctttcgc ttgcccagat gaaaagttta caaaaagccg gtgttgaatt tggtatccac
    15961 agttggtctc atccagattt ttcgcgttta atttatcctg aagcatatac cgagcttgat
    16021 ttgtgcttta aacaaatgga agaattatcc gtgccttgta agcgttattt tgcctatcct
    16081 tatggtcgcc ctgcccaaaa tgaaatagaa acaagattga ttaaaaattt tcagctctct
    16141 gctacttttg gaatcagcta tggtcttggt gataacagtt taccctatac acgttggcaa
    16201 agaattaaaa tggatggtag gaaattgacc aatcgggaac agttcctgct tttgcccttg
    16261 ttgcgttcca taaaagcatc caggtttaat tttttatgac cgcacacaac ttttttcatt
    16321 gacaattttt tgcaaattca cataatgaca aaagcaataa aaattataca tagctttttt
    16381 gaaaataatg agttagaggt tgtatatgca taataatatc cctttcctgt ggattattgc
    16441 acccttaggt gctattctgg ctttaatttt tgcagcaatt ttctttcgtc aggttaaaaa
    16501 acaagaccct ggcaatgcta aaatgcagga aattgcaggt tatgttaggg aaggtgcttt
    16561 tgcctattta aagcaacaat ataaaggtgt aggtatattc tttatcattg ctttcagcgt
    16621 ttttcaaata atggcttggg gattgggtgt tttacattgg ttaattccct gggctttttt
    16681 aaccggtggc ttttttagtg gtttagctgg ctatatcggg atgaatatgg ctacccttgc
    16741 ttccaataga actactcagg catgtactaa aggacttaat aaaggtttgc ggatagcttt
    16801 tagagctgga agtgtgatgg gtttaaccgt tgtgggtctg gctttaattg atatatctgc
    16861 ctggtttttt attctcaatc tgcttaaatt ccctctgcaa caaattaccg ttataatgct
    16921 ttctttcggg atgggagctt caacacaagc cctttttgct cgtttgggag gaggtattta
    16981 tactaaagca gctgatgtgg gtgccgatct tgttggtaag gttgaggcaa atattccgga
    17041 agatgaccct cgtaatcctg ctaccatagc agataatgtt ggagataatg ttggtgatgt
    17101 tgcaggtatg ggagccgatt tatatgaaag ttattccggt tctattttgg ctacagctgc
    17161 cttaggaatg agtgctgtaa ctatgttagg tcccgcttat gccgactggg caatgaaatt
    17221 tgtggtagca ccaatgattt tagccggttt gggaattctg ctttctatta taggtatttt
    17281 caccgtcagc accaaagaaa atgcgggtac taaagaactg atgtttgctt taaataaaag
    17341 tgtttattcc agttctattc tgattgctat atgtgcttac tttgtaacta aagctttgct
    17401 tccccctgat tattattttg gggtatttat ttcaacggta attggattgg cagctggaat
    17461 tttaattgga ctttttaccg agttcttcac ttcacatagc tataaaccaa cacgggaaat
    17521 agctaatcag gcagattatg gtcctgcaac tgttattctg gaaggaattt ctgttggaat
    17581 gcgttctaca gcagctcctg ttttaataat tgtaataggc attttatctg cttttatttg
    17641 cagtcacggt ttttcttcta ttgaaatggg tttatatgga atcggttttg gagcggtcgg
    17701 aatgcttgct actttaggtg taactttggc aatggatgcc tttggtccta ttgctgataa
    17761 tgcgggtgga aatgcacaga tgtgtaattt gcctgaagat gttagaatga tgacagataa
    17821 cctggattcc gttggtaata ccactgcagc tacaggaaaa ggttttgcta tcggaagcgc
    17881 tgctttaact gctatggctt tacttgctgc ctatctggaa gaagttcgcg gagctttaca
    17941 tttagtagga actaaagcag ggaaagctgt ctttttgcat actaattata ataccaaatt
    18001 cactgaaaat attcccttag caaaggcatc aattgccgat tttattcaat tcttccaaat
    18061 aaatgtgtta aatcccaaat ttttgctggg tgttttcatt ggggctatgg ttgcttttgt
    18121 attttcatca ctaactatta aagctgtagg aagagctgcc ggtaaaatgg taaaagaagt
    18181 tcgccgtcaa tttaaagaaa ttccaggaat tatggaagga acgggaaaac cggattatgc
    18241 caaatgcgtt aaaatatcta ctcaggcagc tcagagagaa atgcttatac ctgctttagt
    18301 aggtctctta tctcctatca ttacaggttt aatttttggt gttccgggag ttttaggtat
    18361 ccttgctgga gccctttcca ccggttttgt tttagctgtg atgatgaata atgctggggg
    18421 cgcttgggat aatgccaaaa agtatataga gacggaaaag aaagctaaag gcactgaaat
    18481 tcataaagcc agtattgttg gtgacacagt tggcgaccca tttaaagata ctgcaggtcc
    18541 ctgcatcaat attctggtaa agctgatgag tatgatttct attgtctttt cgtgtttgat
    18601 tgtaagttat tgtctccatg tggaaaaaat caatcctcct tttacaaaag ttcacttaga
    18661 cagaaaggta gaaaataaag ttattatggt agataaggat tccttagatg taaatggaaa
    18721 ttatcctcct accaccacaa ccgaaatcat agtaaagtaa acacggaagg ttgatgtcct
    18781 cgtcaaccaa attcttgcag tttatttcgt tactcaaaag ttcgcgttaa agaaaaaact
    18841 ggattataag tggaacgaca agatgtcgtt cttccgtttt gtgtagtagg aggaccaacg
    18901 tcctccggct acatactttt cacaggttct ctatattctt cagcggttta tgtgctgtag
    18961 ctggtgtaat tccttccagg gtaacataac ccaaaacagg tgttcctgtc ggcttacggt
    19021 tttcatcctc gcggtagaat tcaaccggta tacgaattgg tttaccatca atttccccta
    19081 tttcttttcc ttcatcatct ttttcaaaca atggagaata aaaccaaccc attccgatag
    19141 cagcagttcc tttttcttca ttcacatttt tacgcttgga accaattgct aaacgcattt
    19201 cccacgaaac attgggagaa tttatactgc tggtaacagt tcccaggcgt ttgccatctt
    19261 tataaatacc ccgattagat acaatacctt cggaaacaaa gatatataaa cgcttttgca
    19321 ccccttccgc tatttctttg gctaatgcct cttttcctac aaagtattcc ttgttcatat
    19381 ctaccgcggt ttcaaaaaga ggagcattta caggtgtatg ataagggtca tattcctgcc
    19441 ccatcaaagg taaaccgaaa ggtcctgcag aaatcctgtt ttcatctcta ccacctaaac
    19501 caactaagag tcctccgaag tccaatgcct tggaaataat aattttggca tcttcttcag
    19561 caacggcaac aggaatataa agttcccaac cccatctgtt agtatatcct gttctggata
    19621 aatagtaatg gtgtccgttg cgttcaaact cattaaaagt gaagaaattc atattctttt
    19681 ccggattatt tctgttcttc aacacacttt caccatagac ctcttttagc aatttgtaag
    19741 atagaggacc ttgaacatct atttttagca aggtatcgga aatatctttt gcccaaacct
    19801 cttccccttc ttttttgcat tgcataattc tgtctatatc agcaagatat tcttgttttt
    19861 cgccatctac ttcaattgta tcagtaatat catgtccagc attgataacc agaataaaac
    19921 tggtatcagt aactctcatc agaatcatat catcttgcac accaccctgt tcgttcaata
    19981 gtaaagtata tttacattgt ccaactttca tgtcggtaaa atttccggtt aaagcccgat
    20041 ttaaaagagt tactacatca ggtccgtaaa agagtaattg tgccatatgg tcaatgttat
    20101 agacagctac tttattgatg gcagctgctt cttctaacac agaagttaaa taatttaccg
    20161 ccatagaata ttgaccgaaa ttgctgcgtt tgatgctttg cattggaata ccgagatgtt
    20221 ttgataaaag gggaagatac cattcctctt ccaaattgta gagggctgtt tttctttcct
    20281 gaacaggaaa cttgaaacct aagctgttca tctttaaact ccttatgata tatctttttt
    20341 tgtcatattg gaaaaagcag gtctttttgt ccagaataat cttgtttttg taaatgaaac
    20401 ctaataatta agtaaggaat agtcaatatc tttcactgat ttattagcaa tatgcatttt
    20461 acggataaaa cgaatttacc cggattgcaa tggatgatag gtagagaaga aggttgagaa
    20521 tctctccgct ctctgctctt tcaggatagt catcccaaac ctattattat attctaagtg
    20581 cctgttccaa ttctactgct ttatcggttt tatcatctct atattttata ataatcggac
    20641 aggcaatttg aaaatcagga ttacttttca gtcgtcgtga acctggaact actacagctt
    20701 tagaaggaat ggtaaaacta ttgccgctat cgcgttctaa aaacttttcc cgaacgctgt
    20761 cataaatagg tgtggaggcg gttataacag ttccggaagc aattactgcc ttgttttgaa
    20821 caattattcc ttcatagatt ccagtgtttc cacctacaaa aacatcgtct tctaaaataa
    20881 caggacgcat tcccacaggt tctaataccc caccaataat agctccagcg gaaagatgca
    20941 cattttttcc aatttgggca caagaaccaa ccaaggaatg tgaatcaata agagttcctc
    21001 tatcaacata ggcaccgata ttgatataag caggaggcat aatcgttact ccggcagaaa
    21061 tataacaacc atttctggca gaacttcctc cgggaacaat tctgattcta tctgctaaag
    21121 taaattgctt ttccggtaga gtatccttat caaaaaaagg tttcgcttca ctccattgat
    21181 agatagttaa ttcccccatc cgaaagccaa gaaggatacc cattttcacc cattcattca
    21241 ctttccaaat acttttttct ttttcgcagg agcggatttc tcctttattt aaggcatcta
    21301 taaaagctgc aaataatgct ttatgctttg ctgtccattt ttccggtggg ttattataaa
    21361 gttcaattat ttcctgtttc atccgtattc ccttgctaaa atttgtaaac gctttaaggc
    21421 aatctctaaa atgctctttg gacatccgaa atttaacctc ataaaacctt ctccgtcttt
    21481 tccaaattca gttccgggtt caagagcaag tcccgcttta ttaactataa tttcttgtaa
    21541 gttcgcctct gtcaaacccc agtttctaaa atccagccaa gctaaaaaag tcccttcaat
    21601 agggcttatt ttcactccat gtaattcatt ctgcacaaat tcagtgatat atattctatt
    21661 cttatctaaa taacaaagta attcatccaa ccagttctcg gattcactat atgcagcaat
    21721 taacgctctt atcccaaagg tattaccaag atagagatga agttttgtat taagctcatt
    21781 gaacttattc tttatttctg aatttgaaac aattacaact gccgttgcca aacctgcaat
    21841 attaaaactt ttagaagggg aaacacctgt taaacaaatt tcggaaaaat cattcaatga
    21901 agccaaagga agatgtttgt aaccaggata aacaatatcc gcatgaattt catcggaaaa
    21961 gacagggact ttatatttag cgcataaact gcctatttta gttaattctt cttttgtcca
    22021 aactctactt acaggattat gcggactaca gaaaataaat aattttgcct gtttcaattt
    22081 ctcttcaaag tcctgccagt caatactata aatgccattt gtattcacca gagcagaggt
    22141 taaaagtttt cttttatggt ccagaacagc atcataaaaa ggacggtaaa caggggtctg
    22201 aataagaatt ccctctccgg gttcagttaa cgaaagaaca gctaatgaaa tagcaggaac
    22261 caaaccggga acagctataa tccagttctt ttgcaccttc caatcaaagc gtctttcctg
    22321 ccaggaaata attgcctcat aaaaatcatc cagacgcaag ttatagccaa agacaggatg
    22381 tttcaagcgc tccgataaag cggaaataat ctcatcggaa acagcaaaat ccatatcggc
    22441 aacccataaa gggataagct cacttttgcc atacagcatt ttcagaccat cgtatttata
    22501 gcaaccgcta ttacgacggt ctattatttc atcaaacctg ctctgtttca ctttttcccg
    22561 ctaaatttct taaattgcta acaactttac ccaataaatc attcaattga gcttggggag
    22621 caaaatcagc caggttaagc aaaatctcca ggattttagg aaatgaagct gaggttttga
    22681 atatcttttc cctgtcttta tctttgtatt tcaaaataat gctgaaggaa atctgtttcg
    22741 tcaaatcaat ggaagttata ttttcatatg gaataatgat tttgggttta cataaaaatc
    22801 caaaacatat tctatcttca tagaaggtca cgctataaag agaagtaagt tgtttgaaca
    22861 ccgaatccaa cgctacaaac agaatgatta aaggcagaac cttaaaaaac attggtgtag
    22921 tgccagtcac aaattttacg aagaaaaaga ttgcatagat acaaattgcc aaagccacga
    22981 ataacataat aaatctcgtc cagggagaat aacgataagt ggctggcaat tcgttacgga
    23041 ttgattttac cttaatttca cgcggcattg atattccttc caaagttttt ttacgctggt
    23101 gaggacaact tccggcagag gaataatatc aaacaaatta tgtttatcct gttcgctgtg
    23161 ataatcactt cctcctgtca tatacagtcc tttttccgtt gcaattttga tgaaattttc
    23221 tatttcccac tgataatggt ctggatgcca aacctctaaa ccgtcaatgt ccattgtaat
    23281 aatatcttcc agataggata gttttgccag ttttcccgga tgagcaataa cagcgaagcc
    23341 atctgcctga tgaataatag aaataacatc agaaacagaa acctccggct tgggaacata
    23401 agcaggtttg ttgtctccaa tatatttttc aaatgcttca ttcctcgttt ggcaaaactt
    23461 atgattcacc aaaacctgag caatatgagg acgcacaatt aattctctgc ttccagcaat
    23521 agcaattact tcttccaaag atattggcat acccatatct gtcagcaatt taattatttt
    23581 ttgggcgcgt tcttttctgc ctttcaaata catctctgtc atagctcgta aagcaggatt
    23641 attaaaatca cagccataag ctagaatatg cacatcatca cccttataca gagaacttat
    23701 ttccattcca ggtaagattc tgagaggcaa tatattatct ggaatttgcg tataggcatc
    23761 tgcagtatcg tggtcagtta tggaaattaa atccaagcca atttcctgag agcgttttat
    23821 cagttcctta gggcttatat ttccatcgga cacattagta tgcaaatgca gattaacacg
    23881 ctgaatgtca tttatttctt ttactatcat aattcaaatt ctgggttgac ttattttggc
    23941 tctgctaaaa atagtcaatg tatttttttt atcgggagaa tgtgaaatgg aatacgaccc
    24001 cttaaaaaac cgtctggcaa gagctataga tatgtttcct gctttgcgca aactttttta
    24061 tatggctttt aaccttattt ttctccgcca gagatatgtg ctgaaagaga ttcgcaattt
    24121 gttccgggaa acggataaaa ttaatttata tgatgccgga gctggctttt gtcaatattc
    24181 cgcttatgtt ctatcgcatt ggctcaattc caatgctttt gctacagacc tgaaaacaga
    24241 ttatttaaga tccttttctg catacgcgga tatttattat ccaggcaggt tcacttataa
    24301 aacaaccgat ttacaagctt ttatcccttc ccaaaagttt aatttagctt tagcaattga
    24361 tattatggaa catattgaag atgatgtttc cgccctgaat aatttgtata atgccctgaa
    24421 aaaaaaggga tatttgcttc tttctactcc gtcagaccta aatgaaacag caaaatttac
    24481 ttccgaacac attcgtgctg gctataataa aaaggaactg gaaaataaac tgaaccggat
    24541 tggtttccga attattaaat ctatttatac ttatggaact tatggtgctt tatcctggaa
    24601 attattaatt aaaatacccc tccagctgat ttctaaaaaa ctttatcttc ttctgccttt
    24661 gtattatctt cttgtttatc cattagctga aatcctaatg cgtttggata tgaagacaga
    24721 taacaaacaa ggaacaggaa ttctgattgt tgctcaaaaa ccttgaaccc gaaaggaacg
    24781 agacacggaa ttggaaggta gaatgaattt aacaaggaaa cttggtagat ttatggttga
    24841 gaaggttgaa agcccaaagg gcaaagatta tctcaacctt caaaccctct caaccttctc
    24901 aaacctttca atcctgttaa ttccagatta ttattcaaaa ctgcaatgtt tccggaacaa
    24961 ccgggcatag catcagtgct ttatcctgtc caaaatcgct ccacaattca tagagtttga
    25021 tataatcaga attaacagca ttcattactt taacaggtgt aaaaacattc aattcaccga
    25081 tattaatgct tatttttaat ccctcttttt tagtagtggc atcaacgagg gttaactttc
    25141 cttttttatc gataagttca ctcatacttt ccttagcttt ttccaatccg ccaagttcat
    25201 caacaagtcc aattttttgt gcttgttctc ctgtccagac ccttccttga gcatattgtt
    25261 taacttgttc caaggtcata ttcttgcgtc cttcatccac cttcttaaca aagtcatcat
    25321 aacaattttc tatactgcga gttaccattt gtttttcttc ttcagtccaa ggacggtaaa
    25381 tagaacccat atcggcatgt tcaccttttt taacggtatc ccagtttact tttatttttt
    25441 gaaacatctc tgtgccattg aacatcaatc ccacaacacc gatagaacca gttaaagttg
    25501 agggctcggc aataatttta tcggcattac aggaaatgta atatccaccg ctggcagctg
    25561 ttccgcccat agaaacaaca atcggttttt tattttctgt ctttgccagt tccagttctc
    25621 ttaaaataat atcgctggct tgagcacttc cgccaccgct atccactctt aaaattattc
    25681 ctttgtattg tttgtctttt cttgctttgc ggataagatc aaccgttgtt tcctgagcaa
    25741 ttttttgccc cggagttcct tttcccgaaa caatattccc gctggcataa ataaccgcga
    25801 ccaaattttc tttgggtttt gcccaagcat attcgcgata ttcagttaat tctttatact
    25861 gacttgaaaa cttaaaatct ttctttaatt gcttattcag cttgtcttca taaatgaggg
    25921 catccactaa tcctttttcc agggcatcat tagcaataaa atagggaccg ccattgataa
    25981 tttcgtcagc tgaagcaact aatctatcac cgcgtcctgt ttccatactt tgtaaaattt
    26041 ggtcgtatag tgattgcaga agggattcat aagcttccct ttccgcggaa gtcattcttt
    26101 cttcggaaaa catattaccc gcatctttgt attcgtgact gcggaaattg agtacttcaa
    26161 tacccaagct ggcaagcata tttttcaaat aaggactgct gatacttaaa ccccgtaaat
    26221 ccacagaacc cgtcggattt aagtaaatct tatcagcaat ggaggaagca aagatataac
    26281 cgccattgcc aatattatta tagtaaaaac aaactttctt gccactgctt ttaaagtcgt
    26341 taaatgcatc aatgagttct tcctgtaaag ctaaagaagt tgaaaaggaa ggatttttca
    26401 gaagaatacc ttctatttcg ggatcatctt ttgcctgttt aatgttatca atgactgact
    26461 ctatggattt atcgccggta tcgtatattt tgattttccc tattgcatat ttcggagcag
    26521 tgtaggttac aatgttgcct ttcaaatcca ttttatacca ggaaggtttt gtatgtccca
    26581 aaaatggttt atgctcccaa tctgtaagct gagcccaagc aattccataa ttatcattct
    26641 tcttagaacg caataagcca cctgcctcaa gatttctggg acataagctg aaattaacta
    26701 aagctgactc ttcttcccaa ttataggtag ctccgacttt tactccatcc aaaatttggg
    26761 tctgaattcc cagaaccggt tttttgaatt cataatcctt gtccatttca tagcgcgcat
    26821 aattagcatc tacagaaagt tccaaacggt aatccgctat agcatccgta aaaacaaaag
    26881 gacgcactgc caatcccaaa cgataatagg gtgattgatg tttgggatta tcccaggtaa
    26941 aggctaaaga agtagaatta tgtggacggt atgtaactcc agttctgaaa gaaccatctt
    27001 caaaaccgcc ttcctgccaa cggtaattcg ttcctgcata taagttgggt aaaatatgtg
    27061 caggaaaaag ttcagctccc aaagctaaag tatgatattt ataactatgg tcgcgttcat
    27121 aaatataact gagcaaatca gtattcaaaa atatccaata gtgattttgc cattctttat
    27181 catcattgag ataagctaaa cctatacccg aagcagaacc ggttcccagc aaagaaggat
    27241 tgctaatagg aatgaacata ttatccacgc tggcaatggg aaaatcctgt ttattcaaga
    27301 atacgaaaat actgtcactc ttcgtttgcg ccatcgctaa cgagcaaaaa aggaatggta
    27361 ataacaggat tagagcttta actatggact tcattgtttc tccttttttc gtttaataat
    27421 actttaactc agggtttagt taaacaaaaa taattttttt tcgggagtgt tcattttatt
    27481 gggtctgttt tctaataaaa taatcggaaa accatag
//
LOCUS       DAOPFL010000011        27422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1727_length_27422_cov_86.319545, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000011 DAOPFL010000000
VERSION     DAOPFL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27422
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1727_length_27422_cov_86.319545"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..2239)
                     /locus_tag="PLG69_01830"
     CDS             complement(200..2239)
                     /locus_tag="PLG69_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPI42280.1"
                     /translation="MSRHLHFYLGIVLMLVSIFLTAENVPVTLSKNNSNNDSDILAEY
                     NGGVITRKDIELKISKLPPQYQSRYKTVGGQMEVLNAIAAEEVFYQKARQMGLENDPI
                     VLEHISAIEKRFYIQEYYKRNVSDLVFITDDDLLSYYNQNLSQFYQYPYVTIDYIQAE
                     NEDAAKKALAELKKGISFATVSDTYNQNSYAKGLKGRIKNVRLNGNIPGIGNDIELEN
                     IIRNAQVDTLNFIGPVKTDYGWHILRIVDWIEGYQKDFNEVKPEIEQRVRPMKERELL
                     NALTDNLKIKYAVIIDSAIVNTISLDPNKRNSNKPILDNIIVSSSEPSLQYTVRQLLQ
                     NFDKLSPQEQLFINKGGGINLLIEQELTQGLMYLEAKANGYEKYFSENEDYLQAKRSY
                     VLRRAFEKLVSDTIEITDEEIAERYEREKERFSTPAYRTIEVLFFDNKKDANTAWKKY
                     SKAYKKHNEQKMQEIIKKYSLKPENAILDHQYNNGIITGIGQDAEFSSQIWNNPVGYL
                     SPVFTSARGDIVFFRTLKETPSSYKSKEETETQLRRMIKSEKEKAKMEEVTEDLFVEF
                     NMKKYPERITLTMTAEELFNYADNAARQRNFKDAIMYYDQIINNFKNNSDDYKASFMK
                     AFLIAEEMKQKDLALQLFKEFLQKYPTGDLNDSAQFMIDSLEGRVPEDFEPLRED"
     gene            2427..2864
                     /locus_tag="PLG69_01835"
     CDS             2427..2864
                     /locus_tag="PLG69_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPI42281.1"
                     /translation="MLRKLKNQKGFTLIEILVVVIIVAILAALAVPRYLRYVEKSRST
                     EAQTAINTIRKTYDIYMQTNGTTEGFSMEQALKEASLGESTLKNWKFEVVGNPPKKFI
                     ATSTQDFAGGEGKQVWYDVTDAKFHGYGIDTWTNPETGGMETD"
     gene            2884..3939
                     /locus_tag="PLG69_01840"
     CDS             2884..3939
                     /locus_tag="PLG69_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPI42282.1"
                     /translation="MTIHELLRFTAEAGASDLHIAAGSHPMVRVNGRMKKLNLPIQSP
                     EEVETLVYGVMNESQQALFKKNLEIDFSTKLSNDVRFRVNAFHQINGVSCAFRVIPNE
                     IKSYEELHLPDILKRLTTKEKGLILVTGPTGSGKSTTLATMIDTINDSRNCHIITIED
                     PIEFVHRSKNSLINQRELGHDTWSFTAALRSALREDPDVILVGEMRDLETVSLALTAA
                     ETGHLVFATLHTGSCTKSIDRIIDMFPKEQQQQVRSMLSESLEAVIAQTLLPTKDGKG
                     RVPAVEIMIANAAVRNLIREEKTYQIPSIIQASTKEGMQTRDQSLYNLVMNNLIERTI
                     AEEFADNPKQFATGAGF"
     gene            3942..4418
                     /locus_tag="PLG69_01845"
     CDS             3942..4418
                     /locus_tag="PLG69_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42283.1"
                     /translation="MPKDIYILPENNKTKKANGVFGLLSSQGGYSLAEVLVVAIVMAV
                     MIITIYIGIMYADRQVTKNYRHRVATLMLTGELEKQYTLYMKEGIFRPFTNMPVIIEQ
                     TDEVTVNGTISISVGKDRENYMSGNYDFTYLIGEIRWIDPATEKEHYVRLREDFYE"
     gene            4429..5076
                     /locus_tag="PLG69_01850"
     CDS             4429..5076
                     /locus_tag="PLG69_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPI42284.1"
                     /translation="MKRMIFNEKGFTLTETVAVIAISSLLILTAAVGIGVFFRKYQEL
                     SAYVDLQKDMVSFLNVLKYGQHIGSRSSDIEFMGITSARELKLIRMTTIPGVSKGIEI
                     KPPISDLYPNDYVRYYLHDGFIKMEYRHRGTDSSSPTILFPEKGLKDRVIIEDFLISD
                     ANANNAIFKYRPNEKLCVINVDFKAKVKIRQNEFKSVHYKTVMAMKNMERPSPTN"
     gene            5096..7054
                     /locus_tag="PLG69_01855"
     CDS             5096..7054
                     /locus_tag="PLG69_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42285.1"
                     /translation="MINILKSESGNIAIAMLLVVIGAMSGLTMSSIAFRDTMATMAEL
                     EDIQCIHFLRTEASRGEAYLKVAAKRDPEIVGGVRTPERKIGMRGSDFQKTYKMQSLI
                     NKIYKELESEVVEVGGEIQASQTGASTTYYKIRSLVEGKTGTSDVYYGRKNLPLTRKY
                     GELSLVQDTGPVFMYFTDNEMSPNDTNVYFYGYDVINGPVHSNTDIQIKQLGGGNNNS
                     WPTFLALVTTAGEIISTPSSYPEEAIFRGGLIEHYDEYEFPENMIEIRQNGQRVGPSY
                     SEDRIMVIDIDDYNVSGWQGNITYRRQQIPLYLNNYPNGYPYPPGSETAINRFPTADT
                     TWVPLAPFSAHNGRAYYVESKLWIKGTIRGFQTWASADTMWLIGDLLYAGTPRGSDPL
                     SNTRDMLGLVSEKSIIIKYAYRNPADSLRIHTNMGSDSSNPVGGIWIYAAMAALGKGN
                     GNSFEDGVFTFEYQHPHGSIPAVKFNPSTDDPDVGPIVFDMIDLHRHYWPQSTAHPWP
                     ADLDFPWYNPIWPEANPYMERGTISIWGGVNQRRRGFVHRSMNDTEYPSNSGVWKPSI
                     DMCGGPCSTTATVVQLFQNPTVNVTLQCRHYPGAGGGQIGYKKNYNYDSRMYRVKPPF
                     WPYFKKQGERLPLEQGSWYLKKPPKNLI"
     gene            7096..8166
                     /gene="pilM"
                     /locus_tag="PLG69_01860"
     CDS             7096..8166
                     /gene="pilM"
                     /locus_tag="PLG69_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPI42286.1"
                     /translation="MKKKKQPRFKETVGIDIGTHSIKIVHLKKLHEGFKLLNYEISPT
                     VATGTEYIPSDLLPERYGPVLCEMLNTLKINPKRIQHLVTSIGGDNTSIKQIKTIFLP
                     DEELESALFFEAKKHIPISGTDMILDYQVISLEEKTNNMNILLAATSKDVLNEHTNVL
                     MTAGLAPNIVDIDSLAVVNSFILNTFVEDGVYILLNLGAHRTNMIIWGPGAKLFARDI
                     PYGGYNFTRDIMRKRQLEWAEAEHHKLEFGLGDNPAVENVQTVSMLDITEKPTEDSIV
                     DEVRRSLRFYVKEAGNSDFRKIYLMGGTAKLKGLKEFIEEKVAIPTEIFMPFINVEMP
                     EKFQDKKDPQLALAIGLAMRLE"
     gene            8182..8775
                     /locus_tag="PLG69_01865"
     CDS             8182..8775
                     /locus_tag="PLG69_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42287.1"
                     /translation="METTFYFKINLNKYGEMRLQAEREKKQFINAVIIFAVGLVILIA
                     AWIYINGMINTRVENRQRYLNELQKELASYETSEDYLSSKDLDKLAETFNNRIFWAKK
                     MVALSNEINEKLAVRKFTYNNGVLTLNGITEVDKNVKELDLIQSFIDRLKANEEISND
                     FPRIKSGFITRQIIKDTAILEFVIECYSKEASGGGIR"
     gene            8772..9323
                     /gene="pilO"
                     /locus_tag="PLG69_01870"
     CDS             8772..9323
                     /gene="pilO"
                     /locus_tag="PLG69_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPI42288.1"
                     /translation="MRERYLIFILCILLITILFFMFTINSVTAKQHKIKELDRKIKTA
                     QEKLNSARIMDQQLSQFSRIIDNSLTREASFSFDEINAFKTTIGRLADQRTITINKLS
                     DSNKFSLPGLVETTYNLELEATYVQIGQFISDLESMDNIIKIQALDISPLAVSDKEVT
                     APNAPNRYRVTLELSVFKVKREA"
     gene            9325..9864
                     /locus_tag="PLG69_01875"
     CDS             9325..9864
                     /locus_tag="PLG69_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42289.1"
                     /translation="MQQKFIKDLAIALIVLSLFAMAVRLVSLYNTWSKIPEKSVHTKE
                     SVSDTLLTKIKSIENSIQDRKMFVFNSSKDPLRQGNIIKDKVDREKEYQEMLKNTFRL
                     AATARDEYGNKIAYIEYQGELLEAHIGDTVKGRHILDIQDNSIRYSYNGTITTTELAP
                     RPAKPIEETTYGSGTSGNW"
     gene            9892..11292
                     /locus_tag="PLG69_01880"
     CDS             9892..11292
                     /locus_tag="PLG69_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPI42290.1"
                     /translation="MKHIRKFKSHIALVLVLSFLACITLSAQQRRDPILDTKVTFNAD
                     DATLSSVFKALSRLSNTNIVLAIDQAGTAEKEEKRVTINIKDVPIETAVSLVASSAGL
                     SYRVIGSNTFLVGQKQNIMEEIGERSDIIFLNNLDAKKVSDALQNTSGKITPLEGQNA
                     LMVYGNPETFKEIEKLVKSIDIQQQQIEIRVRLIEVHLNEAKQIGVDWSKLNHLTTIL
                     AEDPVNQYGTGLPYNYADATGYLPHGDPTDFGVIPDEQYFQRINGFNDIGHFSRQLTA
                     FDVTIDWLLENNAAQLLTDTRVTALNGELATMHIGEVVPFVVTDKENQLQVERSETGI
                     ILNVTPKVNKDGNITMNINPEVSSVTELVGGYIPRTKVRRVSSAVTVPNEHKIIVGGL
                     LNSNITQKINKIPLLGDLPLIGKIFQHRYEMVENTDLIIEITPRLVSMQETAPTPKLD
                     KRLTRTLIQYEEEEEE"
     gene            11294..>12549
                     /locus_tag="PLG69_01885"
     CDS             11294..>12549
                     /locus_tag="PLG69_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42291.1"
                     /translation="MKKYSSLMSILTLLLIILVSFSASCDKRNPPPILAPVPLPPSAS
                     QERYITRMTANPDRIYADYNITYSIVSAEIKDGEGFGVPNQMVKFKTNLGRIISDVPT
                     DSSGVAKTTFYDDGDIGIATISAIVRNYDASGDTLIWQDSTAITVEIKEIPPVENVEL
                     KLKDLAPPYTMSIMQVITVTAITTNAEGQEVPNNTLVTFSCNERGYFVDSGGNEIGTS
                     AVVGTYNGRATVKYNSGTRATTMPGEEPAMITATVGGVSDTKEIVIEPGNPANIRLKT
                     YVQVDNELVETDTSSVNSPNWIFVQAKLTDIYHNACPYQPVKFSTDIGSFLNTTQTVT
                     QNTQLDGIASVRFTPGLSAGAASIQATANNDTLRAVTIFMINSSELYSLDFTQSSQLN
                     LNVANTGGLESAILRVKLRDINGNLI"
     assembly_gap    12550..12649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12650..13704
                     /locus_tag="PLG69_01890"
     CDS             <12650..13704
                     /locus_tag="PLG69_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42292.1"
                     /translation="ESAILRVKLRDINGNLIDAPQKVYFRIANINAPAGANINGYPQQ
                     DSVEVISNGGEAQVSVNSGTESGIVKIKVSWVSENGNRYIYALKTNIVIHSGPPVPGG
                     IISFVSGFDSGQELGGGLWRVQCGAIVKDIYNNPVDWGTTVWFSIVDNANCEIEASAR
                     VGNQNAEGDSTAGVAYTYVTYDGTLTYHTITIRANCGDDPYGVPIFGDTPCILPLNGP
                     EAYIEAQPLVLMFDLNQTNYEFSDIYFYLTDAQGCQISNAKVMFLALDGGHFIYSSNA
                     IYDPASLPNEYWRLLTNQNGYAIARIAFSPVEAPPPVEEIPQPVPMRVRATLVEALLN
                     RETVVTLIKFPGTAPW"
     gene            13819..15528
                     /locus_tag="PLG69_01895"
     CDS             13819..15528
                     /locus_tag="PLG69_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPI42293.1"
                     /translation="MVYNPQFARLGDILVHEGYVTEEQVKDALLKQGNFGLKLGETLI
                     KLGYLTENELLEALHKQLGYDVVQDKELMDLDINIVSSIPEPYAKENKVLALREEGDG
                     VVVAMTDPENLIVSDSLEKILGKNIKPVLIGNSSLQDAIEKYYKSIRTTTEVEDAVGG
                     FEFVAVDEDENEITIAAATEDVNAPVVKMINLIINEAIKAGATDIHIEPLTKISRIRY
                     RVDGALREVMTPPIGMHASLISLVKVMSKLNIAERRLPQDGHISLKTAMKSVDVRVSI
                     TPTVLGEKVVMRLLDKGEFGFKLTTLGFEEDDMEIFKKIIRRPYGIIIVSGPTGSGKS
                     TTLHAALKEIENIESNIITVEDPVEYRLEGVTQIETKEQIGLTFGSALRSVLRQDPDI
                     VLIGEIRDEETADIAIKFSLTGHLVFTTLHANDAPATITRMIDIGIKPYLVGSSLSLV
                     MAQRLVRKICKYCIRDYIPTEQEIQDAGLTPEEASKINFKIGAGCVHCDNTGYSGREG
                     IFELLTINPEIRRIIYEGGNQDLIREAALRNGMRTLHDAAITKMKKGITTIKEVIKMT
                     VVE"
     gene            15553..16806
                     /locus_tag="PLG69_01900"
     CDS             15553..16806
                     /locus_tag="PLG69_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPI42294.1"
                     /translation="MPNFAYIIKDAKGARIEGMIKAETLDMAVDKLAKEGNTIISVKA
                     AAEGAFKGKLSLFDKVMLAIYKFRTGVSLKSLVFFTRQLSTMFSAGLTIEKAISDLEK
                     EEKSKKFAKVLRKLSDDIRKGYSLSEAMQQHPGVFNPLYIALVTAGEVSGTLHTVLDE
                     LADYLEKMEDTRRKVYSALAYPIFIVIFLFFVVWGMFYYIIPMFADVYANFHADLPAP
                     TTLAINISHFITNNVFMFIVIVLLVIFTFFLINLTDKGRYVFDSIKLKIPVIGKVISN
                     SIMSKFSRTFSILMAAGVPIMDTMELTENVVQNAVIESAVRKARVMVKEGYGVANAFR
                     RTGMFPPTLLQMIATGEETGDMDKLLSKAAEFYEKLVDSVIDRLTSLIEPLLIIFMAV
                     IVGSIIVVIYLPIFDLGEAMSQGLR"
     gene            17704..20283
                     /locus_tag="PLG69_01905"
     CDS             17704..20283
                     /locus_tag="PLG69_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HPI42295.1"
                     /translation="MKKYLCILVSIFPLLSFGAPFWNIPSSVSQPDGSVLNLFASGDE
                     YANRLHDANGYTIIQSAVDGYYYYAILEKGEPVPSAYRYGTIDPSAYGLIPHINISRE
                     ARQRKIDFMNAQKRRNERGPNIGNVNNLCIYIRFSDQTEFEIPRSVYNARFNEVGDNA
                     VSLRSYFQKVSYNQLQFMTYHYPACADIINLSYQDTHPRSYYLPYNAVTNPNGYIDFD
                     ARTDREHSLLMNAVYAVASEVPASLNIDADNDGCVDNVCFIIRGPHSAWSDLLWAHRW
                     VLFSHNVSIRNKRIQDYTFQPEDQNDVTTLCHEMFHSVGAPDLYHYEYDGLTPVGCWD
                     IMESGEGHMCIFMKYFYGQWISSLPLAQIGNTYTLNPVTSPTNNVYMYPIPGSNYEFL
                     VFEYRKKGSDVFEEKLPGSGLLIYRIDQRFEGNADGPPEGIYVYRPNGTLTHNGLVAE
                     APFSADNYRIAFNVYTNPQPFLNNGNTFPINLKNITSTGETISFTLASPTESMAPVIS
                     SISPTSGSILPAETIQLNPQITDPANALLRVEYTLDGVLVYTANSELFSGEINGNLLT
                     AGVHNIGITAISSSGLTTNLNVYYRIIDPNLQNWFSWISPEPLWTEYSRGAIPIKVAV
                     NMDLGTQEYLVKGLRFKITPDPWGEPDIPGLVVAQINRFANGAITEQVLLSIGYIFNP
                     GYDPNFIYTISDTTSISGQIAVILDLFEYQNICFDQNASCGNSWITEPNRIWTDALGR
                     GVVGSAGIELLLQKPNVASDDPFMPAVNLSLSSYPNPFTETNKIKYSLPASGKVNLSI
                     YNIKGQKVTTLQAENKKSGYYELEWNGKDSSGNKVSSGLYFCRLETNGKIVTTKLLKL
                     KGM"
     gene            20495..21838
                     /gene="gcvPA"
                     /locus_tag="PLG69_01910"
     CDS             20495..21838
                     /gene="gcvPA"
                     /locus_tag="PLG69_01910"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPI42296.1"
                     /translation="MPYISNTERDRQKILKTIGVNKFEDLLTAIPEKLRMKKPLALSE
                     PLSELEINKNIQAKKSKNISCEKVNSFLGGGIYDHFIPSAIETIVSRPEFLTAYTPYQ
                     AEVSQGTLQYIFEYQTLICELTGMEIANAGMYDGASSIAEAILMAVRKNHLTKAILPA
                     TLNPNYIQVIKAYTEGNGIELLFAPEKDGVTDLSALESMLNDTIGSVVIQTPNFYGNI
                     EDAKAIESIVHKNPKCLLIACVDPVSLAIFMPPAEYNADIAVGEGQALGNNMFFGGPL
                     FGFFATKLDMARLMPGRIVGGTIDKDGKKAYVLTLQAREQHIRRAKATSNICSNESLC
                     TLAAVVYLCLLGKEGLVEIATQSAQKAHYTANELCKIPGLALAYPKTYFFKEFVLKTP
                     KKAEEIIKALLPKKFYAGIDLAPYGKPDELMIAVTEKKTKAEIDSFVQAMREVVK"
     gene            21835..23289
                     /gene="gcvPB"
                     /locus_tag="PLG69_01915"
     CDS             21835..23289
                     /gene="gcvPB"
                     /locus_tag="PLG69_01915"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPI42297.1"
                     /translation="MSKTIFEHSTAGRKGVTLPKREIDIPVATMFPETMLRTKKARLP
                     EVSELDVMRHYITLSQKNHFIEKGLYPLGSCTMKYNPKLHETLIRDSAFTSLHPYQPE
                     ETLQGALELMYELQKDLAEIAGMAGVTLQPSAGAQGEFTGIKIVSAYHKAKGNTHKDT
                     IIIPDSAHGTNPATCSLVGYKTVELASNEKGRCDIAKLRELVNENTAGIMITNPSTLG
                     LFETQIEEIADIIHSVDALMYMDGANLNALLGIVQPGKIGFDLMHFNLHKTFATPHGG
                     GGPGSGPVAVNENLLPFLPVPVITKDEKGYHLSFANEKTSIGKVHSFYGNFAVLIRAY
                     IYIKMLGAEGLQRVSENAILNANYLMKRLNPYYHIEHQEPCMHEFVMDGTWQKKESGI
                     TTLDIAKRLLDKGFHAPTIYFPLIIPEAMMVEPTETESLESLDAFATAMIEIAAECKE
                     NPELVKEAPLTTPVRRVDDARAVKMLEPIFPLQD"
     gene            23320..23949
                     /locus_tag="PLG69_01920"
     CDS             23320..23949
                     /locus_tag="PLG69_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPI42298.1"
                     /translation="MKKYILTLSVLLALFAFAYAQPVKLGFVNTDRLLLDSNEAAEVA
                     RLFALDKQNWTNQIKSLDEEIKQMERDFEIRKLTMNDAAKREAQSRIDTKKAEAGRLL
                     EEYFGDGGKAEQRYRELIDPLTLKIHNLIKKIAEDEKYTMIFDVSMGSVLYALPALDL
                     TDQILLELNKDTIKPTEGTTPEPPKPEGQKPPEIKPPEGFGDEFKPDKL"
     gene            23946..24995
                     /gene="lpxD"
                     /locus_tag="PLG69_01925"
     CDS             23946..24995
                     /gene="lpxD"
                     /locus_tag="PLG69_01925"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPI42299.1"
                     /translation="MKRFNIPINSELLTQICQGELKGKIPSVLCKVSEPEEADEQTII
                     FLEQEKFLEKVKASSAGLIITTEKFISELPERALLLVQKPYYSLMRLISWWLEQENKD
                     FIYTIQSTAIVAEDVRFEGEVAIGDNVVIGSGCILGKGVIISEGCSLGKNVSVGAGTK
                     LYPNVCVYDDCVIGRNCILHSGVIIGADGFGFMLIEGIQQKIPQVGNVVIGDGVEIGA
                     NSCIDRATLGSTIIGSGTKIDNLVQVGHNCIIGEHSILCAQVGLAGSTIVGDYVYLAG
                     QVGIADHLQIGNRAMVGAQSGVSTNIPDDGRYFGYPALEANLTKRIMAVQKNLPEMYH
                     FYLKAKKQETENGEK"
     gene            24995..26389
                     /locus_tag="PLG69_01930"
     CDS             24995..26389
                     /locus_tag="PLG69_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPI42300.1"
                     /translation="MEYKQTIGGESTYTGIGLHSGEISTIRFKPAKADEGIVFIRTDL
                     PGKPKIPADIDHVVDISRGTTIGINGATVATIEHVLSAITGMRIDNICIEINGPESPV
                     ADGSAVVFLNLLRQCGIVQQDSEREYFELDSPISFSAPEDNVDIVIVPSNELKITFMI
                     DYKHPYLGTQYTWLPSLDNYEKDFAGARTFCFINEILELKNMGLIKGGSLENALVIAE
                     PNISEAELKHLQDIFGYHKPITVSQEGILNSHPLRYYNEFVRHKVVDLIGDIALLGVP
                     IKGHILAARSGHKTNVELVKKLRQIQKKHQLQKIYQKDKSQNVVFDINAILRILPHRY
                     PFLLVDKIIEFIPGESLVGIKNVTINEPFFQGHFPGHPIMPGVLIIEGMAQAGGIMLL
                     NQMDNPQNYVAYFASIDNVKFRKPVLPGDTLRYELKVISLKRSLAKMHGETFVEGEKV
                     AEGDFLAMIMEREK"
     gene            26386..27159
                     /gene="lpxA"
                     /locus_tag="PLG69_01935"
     CDS             26386..27159
                     /gene="lpxA"
                     /locus_tag="PLG69_01935"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPI42301.1"
                     /translation="MTIIHPTAIIEEGAVIGDNCYIGPYCHIGKDVVLGSNNNLIANV
                     TILGDTIIGDGNKIFPYAVLGTEPQDLKYKGEPTRLRVGSNNTIREFVTINCSNQMEE
                     DTVVGDNNLLMEYAHIAHNSQIGSGCVIANVVQCGGHIHIGDFATVGGLTAIHQFVHI
                     GAYAFVGGASATNKDIVPFSRGQGNPYKTVGLNSIGLMRKGFASETIAAIKEIYNLFY
                     RSGLNTSQALEKALQIPNPTPEQIIFIQFVQNAQRGISK"
BASE COUNT         8640 a         4883 c         5630 g         8169 t
ORIGIN      
        1 ttcaccgggt taaaacccgt tgttaatatc tgtcgttcct acggaactcg ttctcgggaa
       61 aggagataat gactcttctc ctttctcagg aatgacaaag agcttgtcat tcacaaatgg
      121 atattatata ttttcagaaa tgatataact tacgatgaac tacaagatgt tgttcttccg
      181 caaagaaact gcaactttcc taatcttctc tgagaggttc aaaatcctcc ggaactcttc
      241 cttctaagct gtcaatcata aattgtgcgg aatcattaag gtcaccggta gggtattttt
      301 ggagaaattc cttaaaaagt tgcagagcca ggtctttttg tttcatttct tcggcaatta
      361 ggaatgcctt cataaaggaa gctttatagt catcggagtt atttttgaaa ttattaataa
      421 tttggtcata atacataata gcatctttga aattgcgttg tctggcagca ttatcggcat
      481 agttgaaaag ttcttcggca gtcatagtaa gggtgatgcg ttcgggatat tttttcatat
      541 tgaattccac gaagaggtct tccgtaactt cttccatttt cgctttttct ttttcggatt
      601 ttatcattct tcgtaattgg gtttctgttt cttctttgct tttataggaa ctgggtgttt
      661 ctttcagagt gcggaagaaa acaatatctc ctctggcgga agtaaaaaca gggcttaaat
      721 agccaaccgg attattccaa atttgactgg agaattcggc atcctgtcca attccggtaa
      781 taattccatt attgtattga tgatccaaaa tggcattttc cggtttaaga gaatattttt
      841 taataatttc ctgcattttt tgttcattat gtttcttgta tgctttgctg tattttttcc
      901 aggcagtatt ggcatctttt ttattatcaa agaagagcac ttcaatagtt ctatatgcag
      961 gagtggaaaa tctttctttt tcgcgttcat agcgttcagc aatttcttcg tctgtaattt
     1021 ctatggtgtc ggaaaccagt ttttcaaagg cacggcgcag gacataagat ctttttgctt
     1081 gaagataatc ttcattttcg gaaaaatatt tttcatagcc attagcttta gcttccagat
     1141 acataagacc ctgtgtaagt tcttgctcta taagcagatt aattccccca cccttattta
     1201 taaaaagctg ttcctgagga gaaagtttat caaaattctg cagtaattga cgcacagtat
     1261 attgtaaact cggttcgctg gaacttacga tgatattatc cagaatgggt ttattactgt
     1321 ttcgtttatt aggatccaag ctaatggtat ttacgatagc gctgtctata ataacagcat
     1381 acttaatttt cagattgtcc gttaaagcat ttagcagctc ccgttctttc ataggacgaa
     1441 cacgctgttc aatttcgggc ttaacttcgt taaaatcctt ttggtaacct tcaatccagt
     1501 ccactattct taaaatatgc caaccgtaat cggtttttac gggaccaata aagttaaggg
     1561 tatccacttg agcattgcga ataatgtttt ccaattcaat atcgttgcca atacctggaa
     1621 tattaccgtt taaacggaca tttttaattc tgccttttaa gcccttggca taactatttt
     1681 ggttatatgt atcggagaca gttgcaaaag agataccttt tttcaattct gccaatgcct
     1741 ttttggcagc atcttcgttt tcggcttgga tataatcaat agtgacataa ggatattggt
     1801 agaattgtga tagattttga ttataatagg aaagcaaatc gtcatcagta ataaatacta
     1861 aatcagatac attgcgttta taatattcct gaatataaaa acgcttttct atggcagaaa
     1921 tatgttccaa aacaatagga tcattttcca aacccatctg cctggctttt tggtaaaaaa
     1981 cctcttcggc agcaatagca tttagcactt ccatctgccc tcccacagtt ttataccgtg
     2041 attgatactg aggaggcagt ttggaaattt tgagttcaat atccttacgg gtaataactc
     2101 caccgttata ttctgctaat atatccgaat cgttatttga gttattttta cttaatgtaa
     2161 cgggtacatt ttctgctgtc aaaaatatac tgacgagcat caatacaatt cccaggtaaa
     2221 aatgtagatg acgggacatt cttatactcc ttatcatatt gcttaacata atagatgtca
     2281 catttataaa agggcagaag aagtcaagac atttatagaa acataaactg ataacaaaac
     2341 atcggtttta atttttgctg aggttttggc acgcattgtg cattatagaa ataataaaaa
     2401 aaacaatccc ataggaggat aaacaaatgc tcaggaaact gaaaaatcaa aagggtttta
     2461 ccctgattga aatcttagtg gtggttatta ttgtagccat tttggctgca ttggcagttc
     2521 cccgctatct gcgttatgta gaaaaatccc gtagtacaga agcacaaact gcaataaaca
     2581 ccattcgtaa aacctacgat atctatatgc aaaccaacgg aaccacagaa ggtttttcta
     2641 tggaacaagc attaaaagaa gccagtttgg gtgaaagtac tcttaaaaac tggaaatttg
     2701 aagttgtagg taaccccccg aaaaaattta ttgctacttc cactcaggac tttgctggtg
     2761 gtgaaggaaa acaggtttgg tacgatgtta ccgatgccaa attccatggt tacggaattg
     2821 atacttggac taatcccgaa accggaggaa tggaaactga ctaactctta ggaggacagt
     2881 aaattgacaa tccatgaatt actgcgcttt actgcagaag caggtgcgtc tgatcttcat
     2941 atagcagctg gctcacatcc tatggttcgg gtaaatggca gaatgaaaaa attaaatctg
     3001 ccaattcaaa gtccggaaga ggtggaaaca cttgtttacg gagtgatgaa cgaatcacag
     3061 caagctctct ttaaaaagaa tctggaaatt gacttttcca cgaaattgag caatgatgtc
     3121 cgattcagag tgaatgcttt tcaccagata aatggtgttt cctgtgcttt cagagtgatt
     3181 cctaatgaga taaagagtta tgaggaactc catctacccg atatcttaaa acgcttaacg
     3241 acaaaagaaa aagggctgat tctggttaca ggacctacag gaagcggaaa atcaactact
     3301 ttggctacaa tgattgatac tataaatgat agccgtaact gccatattat tactattgaa
     3361 gaccctattg aatttgtcca ccgcagtaaa aacagcttaa tcaatcagcg tgaactgggt
     3421 catgatacct ggagctttac agctgcttta agaagtgcct tgcgtgaaga ccccgatgtg
     3481 attttagttg gtgaaatgcg tgatctggag acggtatctt tagcattaac agctgctgaa
     3541 acaggtcact tggtttttgc tactttgcat accggaagct gcacaaaatc catagaccgg
     3601 ataatagata tgtttcctaa ggaacaacaa cagcaagtgc gttcaatgct ttcggaatct
     3661 ctggaagcgg ttatagcaca aactttgtta ccaactaaag atggtaaagg tcgtgttccg
     3721 gctgtagaaa taatgattgc taatgctgct gtgcgaaatc tgatccgtga agaaaaaacc
     3781 taccagatac cgtctataat tcaagcaagc actaaagaag gaatgcaaac cagagatcaa
     3841 tcgttgtata acctggtaat gaataatctg attgaaagaa caattgctga agaatttgcg
     3901 gataatccta aacaatttgc tacaggagca ggtttttagg tatgccgaaa gatatttata
     3961 tcctacctga gaacaataaa acaaaaaaag caaacggagt gtttggtttg ctgagttctc
     4021 agggagggta tagtttggcg gaagtcctgg ttgtagcaat agtgatggct gtaatgatta
     4081 taacgatata tattggaata atgtatgccg ataggcaagt aaccaaaaat tatcgtcata
     4141 gagttgcaac tctgatgctt acaggtgaac tggaaaagca atatactctc tatatgaaag
     4201 agggtatctt tcgccccttc acaaatatgc ccgtaataat tgaacagacg gatgaagtga
     4261 ctgtgaatgg aactatctcc atatcagtcg gtaaagatag ggaaaattat atgagcggaa
     4321 attatgattt tacctacttg attggcgaaa tcagatggat tgatcctgca acggaaaaag
     4381 aacactatgt ccgtttgcga gaagattttt acgaataggg gatggatgat gaagcgaatg
     4441 atatttaacg agaagggatt tacattaaca gaaacggttg cagttattgc aatttcttct
     4501 ttgctgattt taacagccgc tgtaggaatt ggcgttttct ttcgtaaata tcaggaactg
     4561 agtgcttatg tggatttaca aaaggatatg gtttcttttt taaatgtctt aaaatacggt
     4621 caacatattg gttcgcgttc ctcggatatt gaatttatgg gaattaccag tgcccgggag
     4681 cttaaactta ttagaatgac aactattcca ggtgtcagta aaggaataga aattaagccc
     4741 ccgatttcag acctctatcc taatgattat gtgagatatt atttgcatga cggttttata
     4801 aaaatggaat acagacatcg gggaacagat tcatcatcgc ctactattct ttttcccgaa
     4861 aaaggtttga aagatagggt gattattgag gactttttaa ttagtgatgc caatgctaat
     4921 aacgcgattt ttaaatatag accgaatgaa aagctctgtg taataaatgt tgatttcaaa
     4981 gccaaggtta agattcgcca aaatgaattt aaaagtgtgc attataaaac agttatggcg
     5041 atgaaaaata tggaaagacc atctcctaca aattaaattt gggaggttta atattatgat
     5101 aaacatcctt aaaagcgaaa gcggaaatat tgccattgca atgctcctgg ttgtaattgg
     5161 agcaatgagt ggcttaacta tgtccagtat tgcatttcgt gatactatgg ctacaatggc
     5221 agaactggaa gacattcaat gtattcattt tcttcggaca gaagcaagta gaggtgaagc
     5281 atatctgaaa gtagctgcta agcgcgatcc tgaaattgta ggaggagtta gaacaccgga
     5341 aagaaaaatt gggatgagag gaagcgactt ccaaaaaaca tacaaaatgc aatcccttat
     5401 taataaaata tataaagagt tggagtcaga agttgtagaa gttggaggcg aaatccaggc
     5461 gagccaaaca ggtgccagta ctacctatta taaaattagg tctttggtag aaggaaaaac
     5521 gggaacttcc gatgtatatt atggaagaaa aaatctgccg ctaacccgta aatatggaga
     5581 attatcttta gttcaggata cgggacctgt ctttatgtat tttactgata atgaaatgtc
     5641 tcccaatgat acgaatgtct atttttatgg ctatgatgta ataaacggtc ctgtccatag
     5701 caataccgat attcagataa aacagttagg aggtggaaat aataatagct ggcctacctt
     5761 tttagcttta gtaactacag caggagaaat aatctccacc cccagttcct atcccgaaga
     5821 agctatcttt cggggtggtt taattgaaca ttatgatgaa tatgaattcc cggagaatat
     5881 gattgaaatc cgtcaaaatg gacaaagagt aggcccatca tatagcgaag acaggataat
     5941 ggttatagat atagatgatt acaatgtatc tggttggcag ggtaacataa cttacaggcg
     6001 ccaacagatt cctttatatc tgaataatta tcccaatggt tatccctatc ctcccggttc
     6061 agaaactgcc ataaatcgtt ttcctacagc tgatacaacc tgggtaccgt tagctccatt
     6121 tagtgcacat aacggaagag cttactatgt ggaaagtaaa ctttggataa aagggacaat
     6181 caggggtttt caaacctggg cttctgcgga tacgatgtgg ttgattggag atttattata
     6241 tgcaggtact ccccgaggta gcgatccatt gtccaacacc agggatatgt taggtttagt
     6301 atcggagaaa tccattatta taaaatatgc ctatcgcaat cctgctgatt ctcttcgtat
     6361 ccatactaat atgggttctg atagttccaa tccggtaggc ggaatctgga tttatgctgc
     6421 tatggcagct cttggaaaag gaaatggtaa tagctttgaa gacggggtct ttacctttga
     6481 atatcaacat ccgcatggct caattccggc tgtaaaattc aatccttcaa ccgatgatcc
     6541 ggatgtagga cctattgtat ttgatatgat tgatctgcat cgtcattatt ggccccagtc
     6601 aactgctcat ccctggcccg ccgatttgga ttttccctgg tacaatccta tttggcctga
     6661 agcaaatcct tatatggaaa ggggaacgat aagtatttgg ggaggagtta atcaacgccg
     6721 tcgtggtttt gttcatagaa gtatgaatga taccgaatat ccttccaata gcggtgtttg
     6781 gaaaccttct atagatatgt gcggtgggcc ttgttccact acggcaaccg ttgttcagct
     6841 gttccaaaat cccactgtga atgtaacttt gcagtgtcgt cactatcctg gagcaggagg
     6901 agggcaaatt ggttataaaa agaactataa ctatgattcc cgaatgtata gagttaaacc
     6961 acctttttgg ccctatttca aaaagcaggg agaacggtta cccttggaac agggaagctg
     7021 gtatttgaag aaaccgccta agaacttaat atagaaacaa ataaagtaat aaataatgaa
     7081 agataaagag gaagaatgaa gaaaaaaaaa caacctcgat tcaaagaaac cgtgggaatt
     7141 gacattggaa ctcacagcat taaaattgtt cacctgaaga aattgcatga aggatttaaa
     7201 ctgctgaact atgaaattag ccccacggtt gctaccggaa ctgaatatat tcccagtgat
     7261 ttacttccgg agcgttatgg acccgttctc tgtgaaatgc tgaacacact aaaaattaat
     7321 cctaaacgaa ttcagcattt ggttacttcc atcggaggtg ataacacgag tattaaacaa
     7381 atcaaaacca tctttctccc cgatgaggaa ctggaatcgg ctctgttttt tgaagctaag
     7441 aaacacattc ctattagcgg gacagatatg attctggatt atcaggtcat tagtttggaa
     7501 gaaaaaacca acaatatgaa tatcctgctg gctgctactt ccaaagatgt attgaatgaa
     7561 cataccaatg ttttaatgac cgcgggttta gcacccaata ttgtagatat agattctctg
     7621 gctgttgtaa acagctttat tctgaatact tttgtggaag atggagttta tattttgtta
     7681 aatcttggtg cgcatagaac aaatatgatt atctggggac caggagccaa attatttgct
     7741 cgtgatattc cttacggcgg ttataatttt acgcgggata ttatgcgtaa gagacagctg
     7801 gaatgggctg aagctgaaca ccataaactg gaatttggtt tgggggataa tcctgccgta
     7861 gaaaatgttc aaacagttag tatgcttgat attaccgaga aacctaccga ggattccatt
     7921 gtggatgaag ttcgtcgttc cttgcgcttc tatgttaaag aagcaggtaa cagtgatttc
     7981 cgcaaaatct atttaatggg tggaactgct aaacttaagg gtttaaaaga attcatagaa
     8041 gaaaaagtag caattccgac ggagattttt atgcccttta tcaatgtgga aatgccggaa
     8101 aaattccagg acaaaaaaga cccccaactt gctttggcaa tagggctggc tatgcggttg
     8161 gaataaggga ggatttttct gatggaaact acattttatt ttaaaatcaa cctgaataaa
     8221 tatggtgaaa tgcgtttgca agccgaacgc gaaaaaaagc aatttattaa tgctgtaata
     8281 atctttgctg ttgggctcgt tattcttatt gcggcatgga tttacatcaa tgggatgatt
     8341 aatacaagag ttgaaaaccg acagagatac ctgaatgaat tgcagaaaga attggcaagc
     8401 tatgaaacaa gtgaggacta tctttccagt aaagaccttg ataagcttgc ggaaacattt
     8461 aataatcgta tcttctgggc taaaaaaatg gttgccttaa gtaatgagat aaatgaaaag
     8521 ctggcggtga gaaaattcac ctacaataat ggtgtcctta ctcttaacgg cattaccgaa
     8581 gttgataaaa atgtaaaaga attagattta attcagagtt ttattgatcg gttaaaagcc
     8641 aatgaagaaa tcagtaatga ttttcccaga atcaaatcgg gctttataac ccgacagatt
     8701 attaaagata ctgctattct ggaatttgta attgaatgtt atagcaaaga agcatctggg
     8761 ggaggaatac gatgagagaa agatacctga tattcattct gtgcatcttg ctgattacaa
     8821 tactgttctt tatgtttacc ataaacagtg ttactgcaaa acaacataag attaaagaac
     8881 tggatagaaa aataaaaact gctcaggaaa aactaaacag tgcacggatt atggaccagc
     8941 aattaagcca gttttcccgt attatagata atagtttaac cagggaagcc agttttagct
     9001 ttgatgaaat taatgctttt aagaccacca ttggccgttt ggctgaccag cgaactatta
     9061 ctattaataa actttccgac agcaataaat tctcacttcc aggtttggta gaaacaacat
     9121 ataatctgga actggaagct acttatgtgc aaatcgggca gtttatatcc gatttggaat
     9181 ctatggataa tattatcaaa attcaagcat tggatatcag cccgcttgca gtttccgata
     9241 aagaggttac tgctcccaat gctccaaatc gttatagggt taccttagaa ctctctgtat
     9301 tcaaggtaaa acgggaggca taagatgcaa cagaaattta taaaagacct ggcaatagcc
     9361 ctaatcgttc tctcgctttt tgctatggct gttcgtcttg taagtttata caatacttgg
     9421 agcaaaattc cggaaaaatc ggttcataca aaagaatctg tttcggatac tcttttaacc
     9481 aaaattaagt ccatagaaaa ttctatccaa gaccgtaaaa tgtttgtatt caattcgtct
     9541 aaagacccct tgcgtcaagg aaacatcatc aaggacaaag tggatagaga gaaagaatac
     9601 caggaaatgt tgaaaaacac tttccgttta gccgctacgg ctcgtgatga atatggcaat
     9661 aaaatagctt atattgaata tcaaggtgag cttcttgaag cacacatagg agataccgtg
     9721 aaaggacgcc atattttaga tatccaagat aatagtatcc gctatagtta taatggaaca
     9781 attactacta cagaactcgc tccccgacct gccaaaccaa ttgaagaaac gacttatggt
     9841 agtggcacca gcgggaattg gtaattaaat ataatgatac tgggagtaaa tatgaaacac
     9901 attagaaaat tcaaaagtca tattgcactt gtcctcgtgc tatctttttt ggcttgtatc
     9961 acacttagtg ctcagcaaag aagagaccct atcctggata caaaagtgac ttttaacgca
    10021 gatgatgcaa ctctttcctc ggtgtttaaa gctctttcca gattgagcaa caccaatatt
    10081 gttttggcta ttgatcaagc aggaaccgca gagaaagaag aaaagagggt tactatcaat
    10141 attaaagatg tacctattga aacagccgtc tctctggttg caagctctgc tggtttatct
    10201 tatcgggtaa ttggctccaa cactttcctt gtgggccaaa aacagaatat tatggaagaa
    10261 atcggtgaac gcagtgacat catttttcta aacaatctgg atgccaagaa agttagcgat
    10321 gcccttcaaa atacaagcgg gaagattacc cccttggaag gacaaaatgc cttaatggtt
    10381 tatggcaatc cggagacctt caaagaaata gaaaaattgg ttaaatcaat tgacatacaa
    10441 caacaacaga tagaaataag agtccggtta attgaagttc accttaatga agcaaaacaa
    10501 attggagttg actggagtaa actaaatcat ttaactacaa ttctggcaga agatcctgta
    10561 aaccaatatg gaaccggctt gccatataat tatgctgacg ccacaggtta tctaccacat
    10621 ggtgatccta ctgattttgg agttattcct gatgaacaat attttcagcg tataaacggt
    10681 tttaatgata ttggacattt cagccgtcag ctaactgctt ttgatgttac tattgactgg
    10741 ttgctggaaa ataatgctgc tcaattgctt actgatacaa gggttacagc tttaaatgga
    10801 gaacttgcca ctatgcatat tggagaagta gttcccttcg tcgttacaga taaggaaaat
    10861 cagctgcagg tagaacgttc tgaaacaggt atcattctca atgttacacc taaagtaaat
    10921 aaagacggta atattacaat gaacatcaat ccagaagtaa gttcagttac agaactggtt
    10981 ggagggtata taccccgcac taaagtccgc agagtttctt cggcagtaac tgttcctaac
    11041 gagcataaaa taattgttgg tggattgcta aattccaata ttacgcaaaa gattaacaaa
    11101 attcctttgt tgggcgattt gcctttaatt ggcaaaatat tccagcatag atatgaaatg
    11161 gtagaaaaca ccgatttgat catagaaata actcctcggc ttgtttctat gcaggaaacc
    11221 gctccaacac caaaattgga taagcgttta acccgaactc taattcaata tgaagaagaa
    11281 gaggaggagt aaaatgaaaa agtatagctc cttaatgtca attcttacac tcctgctgat
    11341 catcctggtc agtttcagtg caagttgtga taaacgaaat cctccaccaa ttttagctcc
    11401 ggttccctta cctccgtccg catcacagga aaggtatata accagaatga cagctaatcc
    11461 ggatagaatt tatgccgatt acaacatcac ttactctatt gtttctgcgg aaataaaaga
    11521 tggtgagggt tttggagttc ctaatcagat ggtaaaattt aaaaccaatc tgggcaggat
    11581 tatttctgat gtccctaccg atagcagtgg tgtagctaaa actactttct atgatgacgg
    11641 agatatcggt atcgctacta tttcagctat agttcgcaat tatgatgcca gtggcgatac
    11701 tttaatctgg caggattcca ctgcaataac tgtggaaatc aaagaaattc ctccggttga
    11761 aaatgttgaa ctgaaactta aggatcttgc tccaccctat acaatgtcta ttatgcaggt
    11821 gataactgta actgcaatta ctacgaatgc agaagggcaa gaggttccca ataatacatt
    11881 agtaactttc agttgtaacg aaaggggtta ctttgtagat agcggaggta atgaaattgg
    11941 cacaagtgcc gtagtaggaa cctataatgg acgagctacg gtaaaataca attccggaac
    12001 tcgtgccaca actatgccgg gggaagaacc agcaatgatt acagcaacag ttggaggtgt
    12061 ttccgatact aaggaaatag taattgaacc aggaaatcct gccaatataa gattaaaaac
    12121 ttatgtacag gtggataatg aacttgtaga aacagataca agttctgtta acagtcctaa
    12181 ctggattttt gttcaagcga aattaacaga tatttaccat aatgcttgtc cctatcaacc
    12241 ggttaaattt tccacagata taggttcttt tttgaatact acgcagacag ttacgcaaaa
    12301 tactcaactt gatggaattg cttctgttag gtttacaccg ggattatctg ccggggctgc
    12361 ctccattcaa gcaactgcca ataatgatac tttaagggca gttaccattt ttatgataaa
    12421 ttccagtgaa ttatactcgt tggattttac tcaatccagt cagcttaact tgaatgtagc
    12481 aaatacgggt ggtttggaat cggcaatatt acgggtaaaa ttaagagata taaatgggaa
    12541 tctgattgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggaatcggca
    12661 atattacggg taaaattaag agatataaat gggaatctga ttgatgctcc tcaaaaagtt
    12721 tactttagaa tagccaatat aaatgcgcct gccggtgcca atattaatgg ttatcctcag
    12781 caggattctg tggaggttat ctccaatgga ggtgaagccc aggtttcagt aaatagcgga
    12841 acagaatccg gtattgtaaa aattaaggtc tcctgggtaa gtgaaaatgg aaatcgttat
    12901 atttatgcct tgaaaacgaa tattgtaatt cattcaggac ctccggttcc aggtggaatt
    12961 atttccttcg ttagcggatt tgattccgga caggaattag ggggtggatt atggcgtgtc
    13021 caatgtggtg ccatagttaa agatatatat aataaccctg ttgattgggg aacaactgta
    13081 tggttctcaa ttgtggacaa tgcaaattgt gaaatagaag cgtcggcgcg cgttggtaac
    13141 cagaatgctg aaggtgattc tactgccggt gttgcctaca cttatgtaac ctacgatgga
    13201 actttaacat atcacacaat aactattcgt gccaattgtg gcgatgatcc ttatggtgtt
    13261 cctatttttg gtgacacacc ttgtatttta cccttaaatg gtcctgaagc atatatagaa
    13321 gcacaaccgc tagtattgat gtttgatttg aaccaaacaa actatgaatt ttcggatatc
    13381 tacttttatc ttacggatgc acaaggttgt cagataagca atgccaaggt aatgttcctg
    13441 gcgttggatg gcggtcattt tatttacagt tctaatgcta tctatgatcc tgcttctctc
    13501 cctaacgaat attggcgatt gttaaccaac caaaatggat atgccattgc cagaattgcc
    13561 tttagtcctg tagaagctcc tccaccagtg gaagaaattc cccaacctgt tccaatgagg
    13621 gtaagagcaa cattagtaga agctttgctc aatcgtgaaa ctgttgtcac tctgatcaaa
    13681 ttcccgggta ctgcaccctg gtaaagaata cataataatt ttcccgattt gcgggttgga
    13741 atattcggaa ctttagttct gaaaaaaaca acccgcgaat tgagaataaa ctgaataatt
    13801 ttgttataag gaaaaataat ggtttataat ccacaatttg cccgtctggg agatatcctg
    13861 gtacacgagg gatatgtcac agaagaacaa gtgaaagatg cattgctcaa gcagggaaat
    13921 ttcggcttga aattaggtga gacacttatc aagctgggtt atctaacaga aaatgaactt
    13981 cttgaagcat tacataaaca acttggctat gatgttgttc aagataaaga attaatggac
    14041 ctggatataa atattgtaag ttcaattccg gagccctacg ccaaagaaaa taaagtttta
    14101 gctttacgcg aagagggcga tggagtagta gtagcaatga cagatccgga aaacctgatt
    14161 gtttcggaca gcttggaaaa aatattgggc aaaaatatca agccggtttt aattggcaat
    14221 tcttctttac aggatgccat agaaaagtat tataaaagta ttcggacaac aactgaggta
    14281 gaagatgccg taggcggttt tgaatttgta gcagtggatg aagacgagaa cgaaatcact
    14341 attgctgctg ctacggaaga tgttaatgca ccagtagtta agatgatcaa tcttatcatc
    14401 aatgaggcaa ttaaagccgg agctactgat attcatattg aacctttaac gaaaatatca
    14461 cggattcgtt atcgggtaga tggtgccttg cgcgaagtta tgacgcctcc gataggaatg
    14521 catgccagtt tgatttccct ggttaaggtt atgagtaaat tgaatattgc cgaacgccgt
    14581 ttgcctcaag atggtcatat ttccctaaaa accgcgatga aaagtgtaga tgttcgtgtt
    14641 tccattactc ctactgtttt aggtgaaaaa gtagtaatgc gtcttttaga taagggagaa
    14701 tttggattca aacttaccac attgggattt gaagaagacg atatggaaat ctttaaaaaa
    14761 attatccgtc gtccctatgg cattattatt gtttcaggac ctacaggaag cggaaaatcc
    14821 actactcttc acgcggcact aaaagaaata gaaaacatag aatctaatat tataactgtg
    14881 gaagaccccg ttgaataccg tctggaagga gttacgcaaa ttgaaaccaa agagcagata
    14941 ggtctcacct tcggttctgc tttgcgttca gttttgcgtc aagaccccga tattgttctc
    15001 attggagaaa ttcgtgatga agaaacagct gatattgcta ttaaattttc attaaccggt
    15061 catctggttt ttactacttt gcatgccaat gatgcacctg caactattac ccgtatgata
    15121 gatattggca ttaaacccta tcttgttggt tcttccttgt cactggtgat ggcacaacgg
    15181 ttagtgcgta aaatttgtaa atattgtatc agggattata tccctacgga gcaggaaatt
    15241 caggatgccg gtttaacgcc tgaagaagca agtaaaatca attttaagat tggagcggga
    15301 tgtgttcatt gtgataatac cggttattcg ggaagagaag gtatctttga attgctgacc
    15361 ataaatcctg aaatccgcag gattatttat gaaggcggaa atcaagacct tattcgggaa
    15421 gcagcattaa gaaatggtat gcggactctt cacgatgctg ctatcactaa aatgaaaaag
    15481 ggaataacca caatcaagga agtcatcaag atgactgtag ttgaataata aatttgagga
    15541 aggtaaatcg ctatgcccaa ttttgcctat ataattaagg atgccaaggg agccagaatt
    15601 gagggtatga tcaaagctga aacccttgat atggctgtag acaaacttgc caaagaagga
    15661 aacactatta tcagcgtgaa agctgcagcg gaaggtgctt ttaaaggaaa actttctctc
    15721 tttgacaaag ttatgctggc tatctataaa ttcagaaccg gcgtaagcct aaaatccctc
    15781 gtctttttta cgcgtcagct ttctactatg ttttcagcag gtcttactat tgaaaaagct
    15841 atatcagacc tggaaaagga agagaaaagc aaaaaatttg ccaaagtcct gcgtaaactc
    15901 tcggatgata tccgtaaagg atattctctt tctgaagcaa tgcaacaaca cccgggagtg
    15961 tttaatccgc tatatatagc tttagttact gcaggagaag tttccggaac cctgcatacg
    16021 gttttggatg aattagcgga ctatttggaa aagatggaag ataccaggcg taaggtttac
    16081 agtgctttag cttatccaat tttcattgtt attttcctct tctttgtggt ttggggaatg
    16141 ttctattaca ttattccaat gtttgccgat gtgtatgcca atttccatgc cgatcttccg
    16201 gctccaacta cattagctat aaatatcagc cattttataa ccaataatgt atttatgttt
    16261 atagttattg tgctgctggt aatctttacc tttttcctga taaatcttac cgataaggga
    16321 cgctatgttt ttgattccat aaaactgaaa attccagtaa tcgggaaagt tatttctaat
    16381 tccattatga gtaaattttc ccgaactttc agtattctga tggctgccgg agttcctatt
    16441 atggatacga tggaactaac cgaaaatgtt gttcagaatg ccgttattga aagtgcagtt
    16501 cgtaaagcca gagtgatggt gaaagagggt tatggcgtag ctaatgcttt tcgcagaaca
    16561 ggaatgtttc ctcctacttt attgcaaatg attgctaccg gggaagaaac aggagatatg
    16621 gataaattat tatccaaagc tgcagagttc tatgaaaaac ttgtggattc cgttatagac
    16681 cgcttaactt cgctaataga accactttta atcattttta tggctgttat cgtaggaagt
    16741 attatcgtag ttatctatct gccaatcttt gacttgggag aggcaatgtc ccagggacta
    16801 cgttaagtta agaaaataaa aaaaaatatt gtatgcggag gatattgttc ctccgccttt
    16861 tttatagccg aaagagcttt atttttcgga aggttggcat ccttgttaac caagcgcaaa
    16921 aaagagttga gaaggttgag aaagttgaga gggttgagaa ggtttagaag gttgggaggg
    16981 tttagagagt tgagaaagtt gagattccaa aaagcccgaa gagcgaaact tctatagcga
    17041 tgggtgcgta agcccctcgg gaaaaaaggt tgagagggtt gagaaggttt agaaggttga
    17101 gatttcatca aatccggaag ggcgacacaa aaatgtagat gggtgcgtaa gcccctcgga
    17161 aaattatgcc aaatcggaag ttgccaaccc ctttttcctc aactgcatta tttgggttaa
    17221 aattagatgt ataacttctt atattttaat gccttcctac ttgactccgt aacacttccc
    17281 taacacttct gtaacacttc cgtaacactt ccgttgtctc gttacggaat tgttacttaa
    17341 ttgttacata gtttttacac cttcaagaaa gcaactgaaa actattataa atttctgttt
    17401 gaaaatgcat aaatgtttaa tggcactata tgaattcctg cttttgcaga attgacaaag
    17461 gacttttcat ccataaaata agataatgac caaataaatg cggaattaaa attgcccagt
    17521 tccggaaaaa ataacatccc tgctacttct ttttctataa acttctctac ttatttactc
    17581 ctggacttca taccctttta acttaatgac ttcccgacct caagacccat agacctcttg
    17641 aactcttgac tttatccttc aataggtata ataattgctt tatttataca taaggagata
    17701 aaaatgaaaa aatatctgtg tatcttggta agtatatttc cccttttaag tttcggggct
    17761 cctttttgga atattccttc ttctgtaagt caacctgatg gttccgtttt aaaccttttt
    17821 gccagtggtg atgaatatgc caaccgttta cacgatgcca acggctacac gattatccaa
    17881 agtgctgttg acggctatta ctattatgcg attttggaaa aaggtgaacc tgttccttct
    17941 gcctatcgtt atggcacaat tgacccttct gcttatggtt taattcccca tatcaatatt
    18001 tccagggaag caaggcagag aaaaattgat tttatgaatg cgcaaaaaag aagaaatgaa
    18061 cgcggtccta atatcgggaa tgtaaacaat ttatgcatct atatccgctt cagtgatcaa
    18121 accgaatttg aaattccccg ctctgtttat aatgcccgtt ttaatgaagt tggagataat
    18181 gcagtcagct tacggagcta ttttcagaaa gttagttaca atcaactgca atttatgact
    18241 tatcactatc ctgcctgtgc ggatataatt aatctttctt atcaggatac ccatccccgt
    18301 agttattatc ttccctataa tgcagttacc aatcctaacg gttatattga ttttgatgca
    18361 agaacggata gagaacacag cttacttatg aatgccgttt atgctgttgc ctctgaagtt
    18421 cctgcctctt taaatattga tgccgataat gatggatgcg tggataatgt ctgttttatt
    18481 attcggggac ctcattctgc ctggtctgat cttttatggg cacaccgctg ggttttgttt
    18541 agccataatg tttctatacg caataaacgg attcaggatt atacatttca gccggaagac
    18601 caaaacgatg taactactct ttgtcatgaa atgtttcata gtgttggcgc tccagacctt
    18661 tatcattatg aatatgatgg cttaacaccg gttggttgtt gggatataat ggaaagtggg
    18721 gaaggtcata tgtgtatttt tatgaaatat ttttatggtc aatggattag ttctcttccg
    18781 cttgctcaaa ttggtaatac ctatacctta aatcctgtta catctccaac caataatgtg
    18841 tatatgtatc ctattcccgg ctccaattat gaatttcttg tgtttgaata ccggaaaaag
    18901 gggagtgatg tttttgaaga gaaattaccc ggctcgggac tattgattta tagaattgat
    18961 caaaggtttg aaggcaatgc tgatggaccc cctgagggaa tttatgttta tagacccaat
    19021 ggaactttaa cccataatgg actggttgct gaagcccctt tcagtgctga taattataga
    19081 attgccttta atgtttatac caatcctcag ccctttttaa ataatggaaa cacttttcca
    19141 attaatctca aaaatatcac ttctacagga gaaaccatta gttttacact ggcttcacca
    19201 actgaatcaa tggctcccgt aattagttct atatctccta ccagtggttc tattttacct
    19261 gcagaaacaa ttcagctaaa tccccagata actgatcccg caaacgcttt gttaagagtg
    19321 gaatatacgc ttgatggtgt tttagtttat acggcaaata gcgaactttt ttccggggaa
    19381 attaacggta accttttaac tgcaggagtt cataatatag gaataaccgc tatatcctct
    19441 tcgggattaa ctacaaacct gaatgtttat taccgaataa ttgatcctaa cttgcaaaat
    19501 tggttttcct ggatttctcc tgaaccgctt tggacagaat actcccgggg tgctattcca
    19561 ataaaagttg ccgtaaatat ggatttggga actcaggaat accttgtaaa aggtcttcgt
    19621 tttaagataa ctcctgatcc ctggggtgaa cctgatattc cgggacttgt tgttgctcaa
    19681 ataaatcgtt ttgccaatgg ggcaattaca gaacaggttt tattgagcat cggttatatt
    19741 tttaatcctg gttacgatcc caactttata tacacaatat ctgatacaac aagcatttca
    19801 ggtcagattg ccgtaatatt agaccttttt gaatatcaaa atatctgttt tgaccaaaat
    19861 gcctcctgtg gtaacagctg gattactgag cccaatagaa tatggactga tgctttaggt
    19921 agaggcgttg ttggctctgc aggaatagaa ttgcttttac aaaaacctaa tgttgcttcc
    19981 gatgatcctt ttatgcctgc tgtaaattta tctctttcca gctaccccaa tccctttacc
    20041 gaaaccaata aaatcaaata ttccctgccc gcaagcggta aagtaaatct atccatttac
    20101 aatattaaag gtcagaaagt tacaactctg caggcagaaa ataaaaaatc cggatattat
    20161 gaactggaat ggaacggaaa ggattcttca ggaaataaag taagtagcgg attatacttt
    20221 tgccgtctgg aaacaaacgg aaagattgtt acaacaaaac tgctgaaact gaagggaatg
    20281 taaaatgaag aatttaaaat taaagagggt ttagagggtt gagaaggttg agagggttga
    20341 gaaggtttag agggttgaga aggattagat gctctctgcc ctttgctctc tgcgttgctg
    20401 atataatagc attctaaaaa gcaattgaca tattcaacgc ttttaatttt caagtaaaga
    20461 atagaaagaa atgtaattaa cataaggagc aataatgccc tatatatcta atactgaaag
    20521 agataggcaa aaaatcctga agacaatcgg cgtaaacaaa tttgaggatt tgcttacagc
    20581 aattcccgaa aaattaagaa tgaaaaaacc gcttgcccta tctgagcccc tttcagaact
    20641 ggaaataaat aaaaatatcc aagctaaaaa gagcaaaaat atttcctgcg aaaaggtaaa
    20701 ttccttttta ggcggtggaa tttacgatca ttttatccct tctgcaatag agacaattgt
    20761 ttcccgtccc gaatttctta ctgcatacac tccttatcag gcagaagtta gccagggcac
    20821 acttcagtat atatttgaat atcagacctt gatttgtgaa cttacaggga tggaaattgc
    20881 caatgctgga atgtatgatg gcgcaagttc aatagcggaa gcaattttaa tggctgtgcg
    20941 taaaaatcat ttaaccaaag ccattttgcc tgcaacctta aatccgaatt atattcaagt
    21001 aattaaagca tacactgaag gtaatggaat tgaactttta tttgctcccg aaaaagatgg
    21061 agtaacggat ttatctgcct tagaaagtat gctaaatgat acaattggca gtgtagttat
    21121 tcagacccct aatttttatg gcaacataga ggatgccaag gcaattgaaa gtatcgtgca
    21181 taaaaaccct aaatgtttac ttattgcttg tgttgatcct gtttctttag ctatctttat
    21241 gccacctgca gaatataatg cagatattgc agttggagaa ggtcaggcgc ttggcaataa
    21301 tatgtttttt gggggtccgc tctttggttt ttttgctact aaactggata tggctcgttt
    21361 aatgcctggc agaattgttg gcggaactat agataaggat ggtaaaaaag cttatgtttt
    21421 aactctgcag gcaagagagc aacatatccg tcgggcaaaa gccacttcca atatctgttc
    21481 caatgaatcg ctttgcactt tggcagcggt tgtttattta tgtttattgg ggaaagaagg
    21541 tcttgttgaa atagccactc aatccgctca aaaagcacat tatacggcaa acgagctctg
    21601 taaaatcccc ggcttggctc ttgcctatcc aaagacatat ttcttcaaag aatttgtgct
    21661 taaaactccg aaaaaagctg aagaaataat taaagctcta ttacccaaga aattctatgc
    21721 cggaattgac cttgctcctt acggaaaacc tgatgaactg atgattgccg taacggaaaa
    21781 gaagaccaag gcagaaattg acagtttcgt ccaagcaatg cgggaggttg taaaatgagc
    21841 aaaaccattt ttgaacatag cactgccggc agaaaagggg taactttacc caagcgggaa
    21901 atagatattc ctgtggcaac aatgtttccg gaaacaatgc taagaaccaa aaaagctcgt
    21961 cttcccgaag ttagcgaact ggatgtgatg cggcattata ttactctttc ccagaaaaat
    22021 cattttatag agaagggttt atatcctttg ggcagctgca caatgaagta taatcccaag
    22081 cttcacgaaa ccttaattag ggattcagct tttacttctt tgcatcctta tcagccggaa
    22141 gaaacactgc aaggcgcttt ggaactgatg tatgaactcc aaaaagacct ggctgaaatt
    22201 gccggaatgg caggtgttac attacaaccc tctgccggag ctcaaggtga atttaccggt
    22261 atcaaaattg tttctgctta tcataaagcc aaaggaaata cgcataaaga cacaatcatc
    22321 attcctgata gcgcgcatgg aactaatcct gccacctgta gtttggtggg ctataaaaca
    22381 gtggaacttg cctctaatga aaaaggtcgt tgtgacattg ccaaattgcg tgaattggta
    22441 aatgaaaata cagcgggaat tatgattacc aatcccagta ccttgggact ttttgaaacc
    22501 caaattgaag aaatagctga tattattcac agtgttgatg ccttaatgta tatggatgga
    22561 gctaacttaa atgccctttt gggtatagtt cagcccggga aaatcggttt tgatttgatg
    22621 cacttcaatt tgcataagac ctttgccaca ccccatggag ggggaggtcc cggttcaggt
    22681 cctgtagctg taaatgaaaa tctactgcct tttttgcctg ttcccgttat tactaaagac
    22741 gaaaagggct atcatctttc ttttgcaaat gaaaagacct ccattggcaa agtgcatagt
    22801 ttttatggta atttcgctgt attaatccgt gcctatatct atattaaaat gttaggagcg
    22861 gaaggtttac aaagggtttc cgaaaatgcc atattgaatg ccaattacct tatgaaacgc
    22921 ttgaatccat attatcatat tgaacatcag gaaccctgta tgcatgaatt tgtgatggat
    22981 ggaacctggc aaaagaagga atcgggaatt actactttgg atatagccaa acgcctttta
    23041 gataaagggt tccatgcacc tactatatat tttccgctca tcattccgga agcaatgatg
    23101 gtggaaccaa cagaaacaga atccctggaa agtttggatg cctttgccac tgcaatgata
    23161 gaaattgctg cagagtgcaa agaaaatcca gaacttgtta aagaagctcc tttaacaact
    23221 cctgtaagaa gagtggatga tgctcgcgca gttaaaatgc tggagccgat ctttccactt
    23281 caggattaac tttatacctt tttcagaaga ggagaaacaa tgaaaaaata tatcttaact
    23341 ctatcagttc tgttagcact atttgctttt gcttatgccc aacccgttaa gcttggtttt
    23401 gtaaataccg accgtctgct cttggatagt aacgaagcag cggaagtagc caggttattt
    23461 gcgttggata aacagaactg gacaaatcaa attaaatcgc tggatgaaga aatcaaacaa
    23521 atggagcgcg attttgaaat ccgcaaacta acgatgaacg atgccgctaa aagagaagct
    23581 caaagtagga ttgataccaa aaaagcggaa gcagggcgcc ttctggaaga atattttggt
    23641 gatgggggaa aggcggaaca gcgttatcgc gaacttattg atcctttaac cttgaaaatc
    23701 cataacctca ttaaaaaaat tgccgaagac gaaaaataca caatgatttt tgatgttagt
    23761 atgggcagtg tcctttatgc tcttcccgct ttggatttaa ctgatcagat tttactggag
    23821 ctgaataaag atacgattaa accaactgaa ggaacaacac cagaacctcc caaaccggaa
    23881 ggtcaaaaac ctcctgaaat taagccacct gagggtttcg gtgatgaatt taagccggat
    23941 aaattatgaa gcgttttaac attcctataa atagtgaact ccttactcaa atctgccagg
    24001 gagaactgaa aggcaaaata ccatctgtcc tttgtaaagt tagtgaaccg gaagaagcgg
    24061 atgaacaaac aattattttc ttggagcagg aaaaatttct ggaaaaagtg aaagccagtt
    24121 ctgccggctt gattattact acggagaaat ttatttccga gcttccggaa agagctttac
    24181 tacttgttca aaagccctat tattcactaa tgcgattgat tagctggtgg ctggaacagg
    24241 aaaataagga ctttatttat acaattcagt caacagcaat tgtggcagaa gatgttcgtt
    24301 ttgaaggaga agtggcaata ggagacaatg ttgttatcgg tagtggttgt attttaggca
    24361 agggagtgat tattagcgaa ggatgttctc tgggaaaaaa tgtttctgta ggagcgggca
    24421 ccaagctcta tccaaatgtt tgcgtttatg atgattgcgt tatcggcagg aattgtattc
    24481 ttcacagcgg agtaattatc ggcgcagatg gttttggttt tatgctaatt gagggtattc
    24541 agcaaaaaat tccgcaggta ggaaatgttg ttattggcga tggtgtggaa attggagcta
    24601 atagttgtat tgatagagct actttaggca gcactataat cggcagtggc actaaaatag
    24661 acaacctggt tcaggttggt cataattgca ttattggaga gcatagtatt ctttgtgctc
    24721 aagttggctt ggcaggaagc actattgtag gtgattatgt ttatcttgca ggtcaagtag
    24781 gaattgccga tcacttgcaa atagggaaca gagctatggt tggagctcaa tcaggtgttt
    24841 ccactaatat tcccgatgac ggaaggtatt ttggctatcc tgctctggaa gcgaacctta
    24901 caaagcggat tatggctgtg caaaagaatt tacccgaaat gtatcatttt tacttgaagg
    24961 caaaaaaaca agaaactgag aacggagaaa aataatggaa tacaaacaaa caattggcgg
    25021 agaatcaact tatacaggaa ttggattgca ctctggagaa attagtacga tcagattcaa
    25081 acccgcaaaa gcagatgaag gaattgtttt tatccgaacc gatttacccg gaaaacccaa
    25141 aattcctgca gatattgatc atgttgtaga tatttcccgg ggcacaacaa ttggcattaa
    25201 tggcgctacc gtagccacta ttgaacatgt tctgtctgcc attactggaa tgagaattga
    25261 taatatctgc attgaaataa acggacctga atcacctgtg gcagatggtt ctgccgttgt
    25321 tttcttaaat cttttacggc aatgcggaat tgtgcaacag gatagtgaaa gggaatattt
    25381 tgagctggat tcgcctatca gtttttccgc tccggaagat aatgtggata tcgtaattgt
    25441 gccttccaat gaactgaaaa taacctttat gattgattac aaacatccct atctgggaac
    25501 gcaatatacc tggttgcctt ctttggataa ttatgaaaag gattttgcag gagcccgtac
    25561 tttttgtttt atcaacgaga ttctggaact gaaaaatatg ggtttaataa agggtggttc
    25621 tctggaaaat gctttagtta ttgccgaacc taatatcagc gaagcagaat tgaagcactt
    25681 gcaggatatt tttggttatc ataaaccgat tactgtttct caggaaggta tattgaacag
    25741 ccatccttta aggtattaca acgaattcgt ccggcacaaa gttgttgatt tgattggcga
    25801 tatagctctt ttgggagttc ctattaaagg gcatattctg gcagcgcgta gtggacataa
    25861 aaccaatgtg gaactggtga aaaagttgcg acaaatacag aaaaagcacc agctccaaaa
    25921 gatataccaa aaagacaaat cccaaaatgt ggtctttgat atcaatgcca ttttgcggat
    25981 tttaccacat cgctatcctt ttttgctggt agataaaatt attgaattca ttccaggaga
    26041 gtcacttgta ggcattaaaa atgttactat caacgaaccc tttttccagg gtcattttcc
    26101 tggtcatcct attatgccag gagttctcat tattgaagga atggctcagg caggtggaat
    26161 aatgttactt aatcaaatgg ataatcctca aaattatgtt gcttattttg ctagtattga
    26221 taatgtaaaa tttcggaaac ctgttttacc tggagataca ctgcgttatg aactgaaggt
    26281 gatttcccta aaaaggtctt tggcaaaaat gcacggagaa acattcgtgg aaggagaaaa
    26341 agttgctgaa ggtgactttt tggctatgat tatggagaga gaaaaatgac tattattcac
    26401 cctactgcaa ttatagaaga aggcgctgtt attggcgata actgttacat cggtccctat
    26461 tgtcatatcg gaaaagatgt tgtattgggt tcaaataata acctgattgc caatgttact
    26521 attttgggag atacaattat aggagacggc aataaaattt tcccctacgc tgttttggga
    26581 actgaacctc aggatttgaa atataaaggt gaaccaaccc gtttgcgggt aggcagtaac
    26641 aataccattc gcgaatttgt tactatcaat tgcagtaatc aaatggagga ggataccgtt
    26701 gttggcgata acaacctttt aatggaatat gcgcatattg ctcacaacag tcaaatcggt
    26761 tccggttgcg ttattgctaa tgttgttcag tgcggaggac atattcatat tggtgatttt
    26821 gctacggttg gcggtttaac ggcaattcac cagtttgtcc atattggtgc ttatgccttt
    26881 gtgggtgggg cttcggcaac aaacaaagat attgttcctt tttctcgcgg acagggaaat
    26941 ccctataaga cagttggact gaatagcatt gggttaatga gaaagggctt tgccagcgag
    27001 acaattgccg ctatcaagga aatatataat ctcttttatc gtagtggatt aaatacttct
    27061 caggcattgg aaaaagcact tcaaatacca aatcctacac ctgaacagat aatctttatc
    27121 cagtttgtcc aaaacgccca aagaggtatc tccaaataat attgaactta ccgggaaaat
    27181 aatgcctgcg gttgttcagt atgcttttat accatttccg tattcataaa cccaactaat
    27241 gcagttcaga ataaatggat tggcaaattg gaagttgtca gctcattttc cctcaactgc
    27301 attatgggag ttaaaccctt taaaaagata gaatcttacc tgcttgcaac tcatcctcca
    27361 cctatccttt aggttgggtt gatgatgggt tataattaag gatgaggaaa aaatataccc
    27421 tc
//
LOCUS       DAOPFL010000012        25872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1906_length_25872_cov_111.158035, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000012 DAOPFL010000000
VERSION     DAOPFL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 25872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25872
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1906_length_25872_cov_111.158035"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLG69_01940"
     CDS             <1..345
                     /locus_tag="PLG69_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42302.1"
                     /translation="IEDREMIYNPNIVKVVTDTQNYALYFSRSVIPFQRDSQIDLKYY
                     RHIGVYAYSPECLHRFVYLSPSILERTEKLEQLRALENGIPIKMVETSYQGIGIDTPQ
                     DLKTIEKQLSVT"
     gene            413..1870
                     /locus_tag="PLG69_01945"
     CDS             413..1870
                     /locus_tag="PLG69_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPI42303.1"
                     /translation="MNKHKSKVLTIIFLAASVFVTAVPVTILHTNDTHGAYLPQNSRF
                     GKIGGYAVLEYYLNAEREKTSAALYLDAGDQQTGSIFASQNYDNAIGGAVIKVFNYLK
                     LDATTYGNHEFDYSQDNTIKHRELANYPFLSTNILDKNNQPFGGIPYKIFSLDSLQIG
                     VMGLTLTELPEKVKRENIAGLTILPYKEAIDKYIEEVDKQSDLIILLTHNGFEADSLL
                     ATVLDNRVDLIIGGHSHTVISEPCQVNGIYIASTGSYLNYLGAINLEVQNDRITAYAS
                     KLIPLLEPENLPITPLNHFVQTMADSIEKETGKVIAHIPVDWVPDKFKETAVSRWAAE
                     ALKQEYYEFYKPDLAIINNGGIRKVIPAGPVTLKDMYELFPFNNYIVLFSCYGRDLLT
                     MEALNKEIAKNKPYDIVQTSSTEWKKKKRLFGLLKPKEVYYINGKPVDPNKIYRVVSF
                     DYVLGQWDKYLGFKPFDVQETGELFTEVMIRQVKK"
     gene            complement(2557..3315)
                     /locus_tag="PLG69_01950"
     CDS             complement(2557..3315)
                     /locus_tag="PLG69_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPI42304.1"
                     /translation="MKISACVFPVLEKIDQAIASMHRYIEEANAQKSDLIIFPEAALG
                     GLNITGIYTQDSQNCLAMDSSEVKSLQQKAIQYSIGIGFGFLEKEKGCIYDSFVLFDK
                     FGQIAVHYHRISRGWLPSEVTSNDYACGNIPGIADTIYGKIGVLICGDLFEPEILNCL
                     IQVETDFYFHIMARSFPCRNNFQKNWDEEEFPFYLNKYQKLGKPIMVCNCVEENAKEE
                     ERYCGGAWFIKGNRIINRMPLLQTGLLNVELLSE"
     gene            complement(3487..3924)
                     /gene="dtd"
                     /locus_tag="PLG69_01955"
     CDS             complement(3487..3924)
                     /gene="dtd"
                     /locus_tag="PLG69_01955"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPI42305.1"
                     /translation="MRITLQRVSKAICYVDNEIVGKIENGLLIFVGFATNDTFEILPL
                     AVRKCLELRIFNDENDKMNKSVLDIQGSVLVISQFTLYANCKRGRRPDFTQSAPPEKA
                     KELYEEFVHLFKQNNINVQTGIFAAKMQIELVNEGPVTINLEF"
     gene            complement(3924..4532)
                     /locus_tag="PLG69_01960"
     CDS             complement(3924..4532)
                     /locus_tag="PLG69_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42306.1"
                     /translation="MTKRLFGPCLLVLLLIFIGSCSTVTTLSFTERDVKDSPNLVKNN
                     SFSPYSTIGEEALKGWMVILNPKETGESPVVIDPEVALEGKTSLRIDASDNSVLILSE
                     PFNVRRYGGYYLRCYFKTNSPHPPRPQLRFIVFKENGKIVNRFKIKAKVNEDWERATI
                     SAGFIKPGARFGRLGIYIPPFKEGSIWIDDTGCFEVHGFKID"
     gene            complement(4529..5749)
                     /gene="rodA"
                     /locus_tag="PLG69_01965"
     CDS             complement(4529..5749)
                     /gene="rodA"
                     /locus_tag="PLG69_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPI42307.1"
                     /translation="MFINRKKFDFVLFTLLLLLIIFGCVIIFSASTTVIGGQIHTENN
                     WWKQIIFAFLSLGVILLLLRLPMPIFDLLILPLYILNILALILVFFTPAVSGAHRWFS
                     LGGINLQPSESAKLLTILLVAKVISKENLNEYKQILYGFLLTLLPALLIILEPDFGTT
                     LVFWASLIAMLIAADVPLYFILLIISPVVSVICSVWLPAIPFWIAILVILLLKSHLSW
                     VAITVSGIINVFIALITPVFWMGLKDYQQNRILTFMDPTRDPLGAGYQIIQAKIAIGS
                     GSVFGKGWLKGTQKNMKFLPEHHTDFIFSVLGEEFGFIGCMLLLLLFVAFFLRLIHNL
                     GELKVRERKVATAGILAYLMFQTFINIGMNIGLVPATGIPLPFISYGGSNLLVNSIAV
                     GVVLKYLNERGFIK"
     gene            complement(5739..7652)
                     /gene="mrdA"
                     /locus_tag="PLG69_01970"
     CDS             complement(5739..7652)
                     /gene="mrdA"
                     /locus_tag="PLG69_01970"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPI42308.1"
                     /translation="MIKNQTTVIFSLVLAILFLLLAVSLFRLQVVEGTKYQRIAESNF
                     VRIRRITATRGEIYDHKYRPIVQNVPSQNLFLTSGKIKNLPALSKFLEHNFGISENEL
                     KDLIFKQRFKTYEEILLADNIPYETVLTLSEELNYYPELSFRIGSTRNYLYPNHFTGY
                     VGRINEQEYELYQKEDYSLNSYIGKTGLERYYEVLLRGKDGREIVQVDAQGRSLQLFE
                     DGGYIEPLNGLSLVLTIDNDLQEFVAGIFPEGMKGAIVVSDIRSGGILAYVSKPDYDP
                     NLFMQKISPETWEYLNRAEKPMMDRIIYGTYPPGSVFKPVTAGIGLDRGLVTPETRLA
                     FCAGGLKVGNRFFRCWYSSGHGSTSIVDALRVSCDTYFYDLSLKINLDDFYNYVRDSH
                     LYGRTGIDLPNERQGFFPNTEWYRKTFGKQEGIIGHKVNLAIGQGEVLTTPLQINAFY
                     GAIANNGLWVQPHLLKQTVGRGRLTREQVIPLTKKKLPMRPETLKTIQQGLWSVVNAP
                     GGTGANARINGATIYGKTGSAENVMGRKTHAWFCGYLVTNKPEIVVTVFLENAGGGGA
                     MAAPLTAKIFNYYMGNLEIIKQIAPLPPQFRTAEGIEAEEQEQGIEDSGFLTPETETE
                     PSVSQPQEESNVH"
     gene            complement(7645..8139)
                     /gene="mreD"
                     /locus_tag="PLG69_01975"
     CDS             complement(7645..8139)
                     /gene="mreD"
                     /locus_tag="PLG69_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPI42309.1"
                     /translation="MVWKGIWSFILGLFFLYLQILVMPALAIWNVIPNILLPWLIYTV
                     WTRPRDLALIVVFIIGLMFDSVNPMTFGLYAFVFCLLALAINEFRKPFETESFVAKML
                     TIGIVNLLFSIIQLLVFGVTFSFASPLLTKSLLGFGYNVLLSLIVFWGLQLLSKVRLN
                     ISND"
     gene            complement(8139..8831)
                     /locus_tag="PLG69_01980"
     CDS             complement(8139..8831)
                     /locus_tag="PLG69_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42310.1"
                     /translation="MSKSKQTEPIITIGGVKFERIPVKTKILTPQDDIGEVIKEYALP
                     LMQPGDILTISESPLAITQGRAIPVAEIKVSPLAKLLSRFVAKVPYGIGLGAPTSMQC
                     AIEETGVLRILLAAFIGAIGKLLGRKGDFYRVAGIQAALIDAATTSPVPPYTDMVIKG
                     PVHPKEVCEDLHSRFGYEFAVMDINDIGGCWMIGSSPNVSKHFMEKVMKDNPQGQGDE
                     LTPFCIVRRVDN"
     gene            complement(8824..9588)
                     /gene="mreC"
                     /locus_tag="PLG69_01985"
     CDS             complement(8824..9588)
                     /gene="mreC"
                     /locus_tag="PLG69_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPI42311.1"
                     /translation="MLKKNLVPLILIVLSLLMLIGSNESRLKKAGWMGNTVFFPFVRS
                     LKAVETRQELKQEVFSLRKRLAETTLENLALQNELKQYTSAFSIAFDIGEADLVQAEI
                     IGISGQYQELNLIVDKGIGSGIQIDDPVISANGIVGKIIQVSSDHSIVLPFSNPRFQL
                     PVMDSRTSVQGILQADISGSIAMNLIKLGSDIAVGDTIVTSNLSRLFPKSYPVGTIKR
                     IRESQDNLFISADINPFTLVENLEHIFILKKAVKNE"
     gene            complement(9656..10693)
                     /locus_tag="PLG69_01990"
     CDS             complement(9656..10693)
                     /locus_tag="PLG69_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPI42312.1"
                     /translation="MSVFDFFGMMANDIAIDLGTANTLVYKKNGGIVINEPSVVAVST
                     DNKKIIAIGNPAKLMLGKNPDEVRVIKPLKDGVIADFQVTELMLRNLILRAQKKRLLV
                     RPRVIVCVPSGITEVEKRAVRDSALHAGSREVHLISEPIAAAIGADLPIDMACGNMIM
                     DIGGGTSEIAVISLSHIVVQNSIRVGGDKIDNDIINYLRKKSNLYVGVQTAEKIKTTI
                     GSAYPLKQELTMDVRGRDIVSGYPVTIKITSEEIREAISETVATIIDAIKRLFERTAP
                     ELSADIAERGIFLTGGGALLKGLDEKIRKTVDLPVHVVPDALECVVKGAGKVLDDIDR
                     YRQVLIKRIED"
     gene            complement(11189..13714)
                     /locus_tag="PLG69_01995"
     CDS             complement(11189..13714)
                     /locus_tag="PLG69_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42313.1"
                     /translation="MRRIRFLIFALLLIMLSPIYGKHSDKYLLANYSYFRCHQNIPDF
                     YYTLLDSMQTAYYNASVITFLPGDFPQRSDLLLKEMDKHKLDVLLYDMAFTEGSKNPE
                     YGSEAFSIANYWRFEAEYDSTFRENLLDDMYFYNNSYTTGTPVFNELASGKYLLQLKN
                     GQPGYAFNKLEFRWQEKGYTKYNIGNEFRFIQREMIDGRKGDIKDNPAGDDTLYITIA
                     FKCSNIPDDPNTELMRFSFNGFDKANKECQVRHYNTYNSRYGTVSYLTVGEYNKLPLI
                     TKDQEGNSVWQHKEIVLKISLQDLYYAGLLDSNIIWKYLLINLNPQIYWNGKGILELD
                     YVEFEDTMYKRQKTDTELIEAVRNRMYNLCSRYDNIKYFYLTDEPTQGQFSSFRRLEK
                     DIFKDLKTFAPSCSGFYTCSILHRKNVIKPNNMMYDHIGLYSKIVEPELIAFDIYPLW
                     GWMQWNNPTERRGVQRRLDYDMLDQYKHYKELCMKTGAKYMPCPQSYGEWNYTDAENG
                     YWALLRPPKYMQKCLQLLPLCYGADGILTYRIYNRIYEENVMKLGFQEFTTIDVSPSG
                     KVTFRPGWEGLKEANQKIIFYADYLEPREWLDADAILTTGYRNPEQLKSVMVKAIEVL
                     PQKLVQNDVDLYDGYVQCGLFKAEGKYPYFMLVNRRTEYISMVNNPTRSDSLLNVPPD
                     KLDDHFLPAPPQTVKFSIDNSAKEILGKYVALYDPFSEELFYSDSNTPEISIDPGDGR
                     LLQMCATLPKKVEGKAEMNHLAILQGNITLEKKADVTVKSDCKLIIKDNSKITVKKGA
                     KLNIFGEVEIGDNVQIQVHKGGTIYLNETDCKWGKNSKISGEK"
     gene            complement(14276..15172)
                     /locus_tag="PLG69_02000"
     CDS             complement(14276..15172)
                     /locus_tag="PLG69_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPI42314.1"
                     /translation="MEKTILIIYLLLGLLLFSCTKKTDTEIPEDKVEPEQMEKTEEKT
                     ESKSVSALDMTAIESYKYTWSNNTDAMPPVVIIIDDFGQNAGQLLDDFSALPKEIAFS
                     ILPDLPYTQTVARLAEKTNHETLIHIPMQALDHQANPGKRYIRTGMDKYAISDLLQDF
                     YAQIPNAVAANNHMGSEVTSDLATMNIILEELDNLGLYFLDSATTNKSAAFTAGKNLG
                     LKIAKRDIFLDVPDNSDATIISKIEGLAKYKGRNEPVVIITHCHNRDKLNALQKFLKQ
                     IDNMEIELISVSQLFRKLGYPA"
     gene            complement(15154..15645)
                     /locus_tag="PLG69_02005"
     CDS             complement(15154..15645)
                     /locus_tag="PLG69_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPI42315.1"
                     /translation="MPKRILIILGLIGICFLFSCKKTASPTPEPTPKYEFRQDGTLEI
                     ISPEGITKATFAIEIAEKEEELMRGLKYRETMAENQGMLFIFEFPDIYDFWMQDTYLP
                     LDMLFIASDGTINYIYEKAIPFSENRITPSSLHKYVLEVNAGTVKKFNIKTGDKIKWK
                     RQS"
     gene            complement(15657..18536)
                     /locus_tag="PLG69_02010"
     CDS             complement(15657..18536)
                     /locus_tag="PLG69_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPI42316.1"
                     /translation="MKRVFFTLCLLILATAVLAYPLRIQSWNLQEDIKKLNELRVSID
                     YVNLQTGIIHIEVRNDAERDKIHSAGILTELLPNPAEEYFASLAEDIKDGRSYYTLTE
                     YQNFMQQTADQYPNICQLVQFGTSVQNRPLLMLKISDNATIEENEPELKYFGSIHGDE
                     VVGYDMLIRLIQLLTTQYGIDPRITNMVNNTEIWINPMLNPDGYAAGIRYNANGIDLN
                     RNFPMPTGVQHPDGEPWAAETIAVMDFSNAHDFDLALNFHGGSLVINYPWDYTTTLTP
                     DNDLLIEMALTYSRENLPMYNSTEFLHGIVNGAAWYIVTGSMQDWNYHYTDCIEMTAE
                     ISNNKWPPASTLDTFWNENRESMLKYIEFAQNGVKGIVTNSSGTPISAIITVAGNSKL
                     EHTDLPIGDYHRLLLPGTYQITASADGYIPQTANITVPTTGYITQNFTLQPAMLTTFE
                     GQIRTPAGNTLCGASITLKTNPPITVQSDIEGLFSFNVYEGDYQIDISALDNATYTYP
                     VQIRQNDKRNIFIMQNSLFSDNFENGLGNWTATGTWGIVTYNDSNVLTDSPTGNYGNM
                     QNNSITLTNPISLIRVSNPYLSFKCKYALENSYDFVYVEASANGSNWIILDSFTGTVN
                     NWINQSYSLASFAGGQLYLRFRIKTDWGQTADGIYIDDVYIIGINNNIALYGDVTSEG
                     IINMQDIAFINEYAIGLDPIPEIDLRPWEAYRITNADVDGNGTIDAFDSYLLCKYIYV
                     PDYSLPVQTGIPEEVPVPVLTASYNNNLNLNFSNIDELKSLTVSVAPNYINQVHHLGI
                     YTNQPFVQAINYETGTYGYAGYEIEQQSISITLEPNPQDFTLYYTVNGVPGSQFVNTG
                     NAADDPNAPETVTYLLPNSPNPFNPETTLSFMLAKNNTLVALNIYNLKGQLVRHLVSC
                     ILPSGKHSFVWNGLDDTGKEVSSGVYLYRLSTPDYQQTRKMLLAK"
     gene            complement(18655..18753)
                     /gene="ffs"
                     /locus_tag="PLG69_02015"
     ncRNA           complement(18655..18753)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLG69_02015"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(18788..20656)
                     /locus_tag="PLG69_02020"
     CDS             complement(18788..20656)
                     /locus_tag="PLG69_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42317.1"
                     /translation="MQKRILFCLVIVWLIYSPFLAAQTQGLDWTIYSSQQALSIENYM
                     QYKYFPTAGTNLIISSKSNLENRLNFNQEAKNADLNVGFQIARKKFLHTLSSGYLTFY
                     DASDLEPSAYVNKTATLGYQINYTPVESLNIGIFSKGIFRNEQDRYVSGNLLSSEGYW
                     IGSDIHYATYFSNSIAGIGTTGERKKMDWESFDFAQVNANYNFYGNYLNVDCLANYSY
                     RSEDIYILTAPRQNENRSNYSLSDNQLRRNLTFAGSVQYYPDDNVQIQLRDDYSQRKT
                     SYSQNEIRNSTDYYNLAQLQFDYQLFPSLVWQNNLRHTYAIKDYYYGLNTRHTENRHF
                     GSRVNWEYSDGDSLIVDYAIDLQIIKFPEDNNQWDNDLLSHNLLWGWKHYWHERILLG
                     NWFGYGFSKDVYLDSLLSANNKRINSWTLSPQCNILLGDRLAFFQVYKIRADYSNYIY
                     KTGKSNTLYRQLSYQYDLVFDTYPLIARSLDPRWLKLPFRNSPDNAFMIDLGFAYEEN
                     QYAQKRKEFYELQTKNRRWTANIDFRYDIRTFYFSFSPQYSWGTWQELTFNLNSAWLF
                     NNNSLLEFKLSPFIDDFEEIEPLSLHNFSINKFFRCVKQCSESADWRLSTTLKLRF"
     gene            complement(20607..21389)
                     /gene="rsmA"
                     /locus_tag="PLG69_02025"
     CDS             complement(20607..21389)
                     /gene="rsmA"
                     /locus_tag="PLG69_02025"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPI42318.1"
                     /translation="MKALKALGQNFLTDKSIAEKIVALGELQPEDSVWEIGPGTGILT
                     DEIIKNNVQLRAFELDRRLYKYLSERYQNRIILEQTDILQADWNSLIRKDGTPLKLIA
                     NIPYQITSPLLALLEEYSQFFSRIVLMVQKEVAERLSAKPGNKNYAPLTIRLRLVFDI
                     SSKIQVSREKFSPMPKVDSAVILLLPRENKPVIKHPEHFHKLLHAAFVHRRKTLANNL
                     IPVLGKEKTEELAKLSHIDFGKRGEELAEEDFILLSDCLADL"
     gene            complement(21450..21626)
                     /locus_tag="PLG69_02030"
     CDS             complement(21450..21626)
                     /locus_tag="PLG69_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42319.1"
                     /translation="MAKNNRGKGLKKVPNNGRGECPVCHRTGIKLLYEVKVGENTYKV
                     CKSCKGIAAEKLAG"
     gene            complement(21799..22194)
                     /locus_tag="PLG69_02035"
     CDS             complement(21799..22194)
                     /locus_tag="PLG69_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPI42320.1"
                     /translation="MKIIHYDEVELEPVTAEGAEKAQIRWLIAQKDGAPTFAMRMFEI
                     EPGGHTPHHQHKWEHEVYCLSGKGALVTDRGEMAFGADDAIFVDPDILHSFKNTGNET
                     LKFLCLIPHEKPIIKKALNPFADEEANNC"
     gene            complement(22363..23469)
                     /gene="ribD"
                     /locus_tag="PLG69_02040"
     CDS             complement(22363..23469)
                     /gene="ribD"
                     /locus_tag="PLG69_02040"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPI42321.1"
                     /translation="MTDAQIKQYMQRAIKVAEKARGKCSPNPFVGSVIVKNNQVISEG
                     CTLEYGSDHSEIQALKKAGKKAKDATLFVTLEPCSHYGKTPPCAQAIIDAGIKEVYIG
                     IYDPNPLVKGKGIAMLKEAGIDVHYGFLEEKIRTQLECYLCYIQKERPFVTLKTALTL
                     DGKYAAEDGTAHWITGKKAREYAHQLRSENDVVLTGIRTILFDDPRLNVRLPGNYKQP
                     LRVVLDPYLKIPVLSYFVSISAKFPGMIITSPDNLQTDKADILRQKGVKIEALETKDG
                     HFNLHEVLNLLYKMNYYSVLLETGSGIAEAFLKEELIDKIIFIYGAKILGGTKSPLPN
                     LGIESIYKALYIWKTSCRKIENDIVVTAYPVYHI"
     gene            complement(23466..24641)
                     /locus_tag="PLG69_02045"
     CDS             complement(23466..24641)
                     /locus_tag="PLG69_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42322.1"
                     /translation="MLKLFKSSRCQSCYTERAIRVCPRKRKNICWQCCNETRCDTRCP
                     ENCDYAPQKEESKPFPSFKADSRAEAENAIKCHIDLWMGRENPFLKNKTPAKEASENP
                     QEMLAWLSGFQYPVYFPMDYLLKKLDLPFTPAEKKEDPENCVEKWMKTLIQLKWNELR
                     QYSINQSSMEDLAERYTELIQAIPQLGKISTYSILYSGLGEDGISALVYMELNHKIDW
                     TLVLSNITGSWKIRQNIAGSPQAYFKQNQIYTAIAEALGKADDAKVWELINSSLKIYP
                     DSPDLYYYRALYWQLVKQREQAAVDFFNAIALDNSWTEPYLHLSAHYFVKGDYEQAKI
                     WLEELKILQPDDPNVLNNLAAAYAGNGETEKAKELWQEIVQKYPAFEYARKNLEKLQ"
     gene            complement(24642..25385)
                     /locus_tag="PLG69_02050"
     CDS             complement(24642..25385)
                     /locus_tag="PLG69_02050"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPI42323.1"
                     /translation="MNYKKERERISNFIRDYCRKSRFENLVIGLSGGVDSALVSALAV
                     QAIGREHLFAFCLPSRESNPESVKDAFLVAECLGLFMQTINIDELTESYFKKYAPEAS
                     PLRKGNWMARIRMNILYDQAAKHNALVIGTSNRSELLVGYFTQFGDSACAFEPIAHLY
                     KTEVWELARELCLPEKVINKTPTADLWSGQSDESELGISYPILDSVLKYLTEGERKPE
                     VAEEIIVKVQKMMDNSSFKRKMPPFLERQ"
BASE COUNT         8018 a         5184 c         4512 g         8158 t
ORIGIN      
        1 attgaagacc gggaaatgat atacaatcca aacattgtaa aagttgtaac tgatacacaa
       61 aattatgctt tgtatttttc ccgttccgtt attccttttc agcgagacag tcaaatagac
      121 cttaaatatt atcgtcacat tggtgtttat gcctattctc cggaatgttt gcaccgattt
      181 gtatatttgt caccctctat attagagagg acagaaaaat tggaacagct aagagctctg
      241 gaaaacggaa ttcctattaa aatggtagaa accagttatc aaggcatcgg aattgataca
      301 ccacaagacc tgaaaacaat agagaaacag ctttctgtta cttgatatta gtttttgaat
      361 gaaagttttt tgtcattcct gcgcaggcag gaatgcagag aagagaagaa ttatgaacaa
      421 gcataaaagc aaagtcctaa ctatcatctt tttagctgcc agtgtttttg tcactgcagt
      481 tcctgtaact attttgcata ctaacgacac ccatggtgcc tatctacctc aaaattcaag
      541 gtttggtaaa attggcggtt atgctgttct ggaatattat ttgaatgcag aaagggaaaa
      601 gacatcagca gcgctttatc tggatgccgg agatcaacaa acgggcagca tatttgcttc
      661 gcaaaattat gataatgcaa ttggtggagc tgttataaag gtctttaatt atctaaaatt
      721 agatgctaca acttacggta atcacgagtt tgattattcg caagacaaca caatcaaaca
      781 tagggaactg gcaaattatc cttttctcag cactaatatt ctggataaga ataatcaacc
      841 ctttggcgga attccctata aaatattttc cttggattcc ttgcaaattg gtgtaatggg
      901 attaacttta accgaactac cggaaaaggt gaaaagggaa aatattgccg gattaaccat
      961 tctgccttat aaagaggcaa tagataagta tattgaagaa gtagacaaac agagcgatct
     1021 gataatactg cttacccata acggctttga agcggattcc ttattggcaa ccgttttgga
     1081 taaccgggtg gatttaatta ttggaggtca ttcccatacc gttatttctg agccctgtca
     1141 agtgaatgga atttacattg cctccaccgg aagttatcta aactatttgg gtgccatcaa
     1201 tttagaagtg caaaacgacc gcattacggc ttatgcaagc aaacttattc ccttgctgga
     1261 accggaaaat ttacctataa cacccctaaa tcattttgta caaacaatgg cagacagcat
     1321 agaaaaagaa acgggaaaag ttattgccca tattccagtg gactgggttc ccgataaatt
     1381 taaggaaaca gcagtttccc gatgggctgc tgaggcatta aaacaagaat attatgaatt
     1441 ctataaaccc gatttggcta ttatcaataa cggaggcatt agaaaagtta ttccggcagg
     1501 acctgttacc ctgaaagata tgtatgaact atttcccttc aataattaca ttgtcctgtt
     1561 ttcctgttat ggaagagatt tattgactat ggaagcactt aataaagaaa tcgctaaaaa
     1621 caaaccctac gatattgtgc aaacaagttc aacggaatgg aaaaagaaaa agcgtctatt
     1681 cggtttgctg aaacccaaag aggtctatta tatcaatgga aaaccggttg atccaaataa
     1741 aatataccgc gttgttagct ttgactatgt tttgggtcag tgggataaat atttgggttt
     1801 taagcccttt gatgtacagg aaacaggtga actctttacc gaagtgatga ttagacaagt
     1861 aaaaaaatga taaatgaaga atgaaattaa caaggaaaac ctattagaat tgagaattga
     1921 aaattaaaaa aggttgagaa ggttgagagg gttgagatgg tttagttctt gttttaactc
     1981 acaattttta gattgggaaa ccatttattg cacggttgga agtgtagaag caagagaagt
     2041 ggtgataatt gggagaacag gtgaaaattg tgagtcaaaa ctttctcctc ggtaaaaatt
     2101 gtgagttaaa atagaatatg cgctggggtt aaaacctgtc gttatctaac tgccatcact
     2161 acaggattta tcttattata taaagtttat gaatattaaa actatataaa atatggagtt
     2221 acagatataa tattttcttc ttccccctaa attttcctaa ctcctactaa ataaatataa
     2281 ttccattttc ttccttaata gcattgctta ctcattgctt acccattgct aatcccatcc
     2341 gtaatgggta agcaatgctt cagcaattca agctgggaaa agctctgaaa acaatggact
     2401 ttatttaacc cccaaacatc aacccaacct ttaggatggg tggataatgg ataacaatta
     2461 agaacggtgt aataaattgt gtagatggag ctccccaaaa tcacaaactt ttttttcttg
     2521 gggaggaacg gtgtcctcca gctacatttt tgcggtttat tcagacaaca attctacatt
     2581 taacagcccg gtttgtaaca gaggcattct gttaataatc cgatttcctt taataaacca
     2641 ggcgcctcca caatatcttt cttcttcttt ggcattttcc tcaacgcaat tgcagaccat
     2701 aatcggttta ccaagttttt gatatttatt tagatagaag ggaaattctt cttcgtccca
     2761 atttttctga aagttattcc tgcagggaaa actccttgcc ataatatgga aataaaaatc
     2821 cgtttctacc tggataagac aattaagtat ttccggttca aacaaatccc cgcagattaa
     2881 aactccgatt ttaccgtaaa tagtatctgc aatccccgga atatttccgc aagcatagtc
     2941 gtttgaggta acttctgaag gtagccaacc cctggatatt ctgtgataat gaacggcaat
     3001 ctgaccaaat ttgtcaaaca agacaaagct gtcataaata caaccctttt ctttttccaa
     3061 aaagccaaaa ccgattccta tggagtattg tattgctttt tgctgtaagg attttacttc
     3121 agaagaatcc attgcaaggc aattttggct atcctgagtg tagattcctg taatgttcaa
     3181 gccccctaaa gcggcttcag gaaaaataat caggtcgctt ttttgcgcgt ttgcctcttc
     3241 tatatagcga tgcattgatg caattgcctg gtctattttt tctaggacag gaaaaacaca
     3301 ggcggatatt ttcattttgt tgttctcctt tttggcgaag gaacagcgtc ctccagctac
     3361 actttgtttt tgttttagct cacaattttt ttaatcagga aatcagtgca agctcggttg
     3421 taaaatttca cgaggggcta acgccacctt ctgttcttca ttctcttttc tctttgtaaa
     3481 aaaaactcag aattcaaggt tgatagtaac cggaccttca tttacaagtt ctatctgcat
     3541 ttttgcagca aaaatacctg tttggacatt aatattgttc tgtttaaaca gatgcacaaa
     3601 ttcctcatag agttcttttg ctttttccgg aggagcagat tgggtaaaat cgggtctgcg
     3661 tcccctttta caattagcat agagggtaaa ttgagaaata actaaaaccg aaccctgaat
     3721 atctaaaaca cttttattca ttttgtcatt ttcatcatta aatatacgca gttccaaaca
     3781 tttccgcaca gcaagaggta aaatttcaaa ggtatcgtta gtggcaaaac cgacaaaaat
     3841 tagaagaccg ttctctattt tgccgacaat ttcgttatct acatagcaga ttgctttact
     3901 aactctttgc agggtaatac gcatcagtca attttaaagc catgcacttc aaagcagccg
     3961 gtatcgtcaa tccagataga cccttcttta aaaggaggaa tataaattcc aaggcgtcca
     4021 aaacgggcac ctggtttaat aaaaccggca ctaattgtag cgcgttccca gtcctcgttt
     4081 actttggctt ttattttgaa acgattgaca attttgccat tttctttaaa gacaataaag
     4141 cgtaactgag gtcgtggcgg atgaggagaa ttagttttaa aataacatct taaataataa
     4201 cccccatagc gacgcacatt aaatggctca cttaaaatca aaacggaatt atcggaagca
     4261 tcaatccgca gagatgtttt cccttccaaa gccacttcag gatcaataac tacagggctt
     4321 tccccggtct ccttgggatt aagaataacc atccaaccct ttaaagcttc ttctccaata
     4381 gtggaatagg gactgaaact attattctta accaggtttg ggctatcttt cacatcccgt
     4441 tcggtgaaac ttaaagtagt aactgtagaa caggagccga taaaaattaa cagcaagacc
     4501 aataaacaag gaccaaataa acgcttagtc atttaataaa acccctttcg tttaaatatt
     4561 ttagaacaac tcccacggca atagaattta ctaataaatt agaacctcca taacttatga
     4621 acggtaacgg aatccccgtg gctggaacca gaccaatatt cattccaata ttgatgaagg
     4681 tctgaaacat aagataagcc aaaatacctg ccgtggcaac ttttctttcg cgcaccttca
     4741 attccccgag attatgaata agacgcaaga aaaaagccac aaacaacaac agtaaaagca
     4801 tacaaccgat aaaaccgaat tcttccccca acacggaaaa aataaaatcc gtatgatgtt
     4861 ccggtaaaaa tttcatattc ttttgcgttc ctttcagcca acctttgcca aaaacagaac
     4921 cgctaccaat agcaattttg gcttgaataa tttgataacc cgctcctaaa ggatcgcgcg
     4981 tggggtccat aaaagttaaa atccgatttt gttgataatc cttcaatccc atccaaaaca
     5041 caggtgttat caaagctata aaaacattga tgatgcccga aacagtaatc gctacccagg
     5101 aaagatggga ttttaatagt aaaataacca aaatcgcaat ccaaaaagga atggcaggca
     5161 accaaacaga acagatcaca cttacaacag gactgattat caaaagaata aaatatagcg
     5221 gaacatccgc tgcaatcaac attgcaatta gcgatgccca aaaaacaaga gttgtgccaa
     5281 aatcaggttc aagaataata agcaaagcag gcaatagagt taacaaaaag ccataaagta
     5341 tctgcttgta ttcatttaga ttctctttgg aaataacttt ggctactaat aaaatagtaa
     5401 gtaatttggc actttccgag ggctgaagat ttattcctcc taaagaaaac caacggtgag
     5461 ctccactcac tgcaggcgtg aaaaacacta aaattaaagc caggatatta agaatataaa
     5521 gcggtaaaat tagaaggtca aaaataggca tcggaaggcg taatagcaaa agaattactc
     5581 ctaaggaaag gaatgcaaag ataatttgtt tccaccagtt gttttctgtg tgtatctgac
     5641 ccccgataac cgttgtagaa gcactgaaaa taattacaca gccaaaaata atcaacagta
     5701 gtaaaagagt aaagagcaca aaatcaaact tttttctgtt aatgaacatt actctcttcc
     5761 tggggttggc ttacagaagg ttccgtttcc gtttccggtg ttaaaaatcc gctatcctct
     5821 attccctgtt cctgttcttc tgcctcaatt ccttcggcag ttctaaattg aggaggcaaa
     5881 ggtgcaatct gtttaataat ttctagatta cccatataat agttaaaaat cttagctgtt
     5941 agaggagcag ccatagctcc acctccacct gcattttcta aaaaaacggt aactacgatt
     6001 tccggtttat ttgtaaccag atagccacaa aaccaggcat gtgtttttct gcccatcaca
     6061 ttttctgcgg aaccggtttt accgtatatt gtggctccgt taattctggc attggctcct
     6121 gttccacctg gagcatttac aacagaccat aaaccctgtt gaattgtttt cagggtttcc
     6181 ggtctcatcg gtaacttctt ttttgttaaa ggaataacct gttcgcgggt aaggcgcccc
     6241 ctgcctacag tttgttttaa taaatgtggc tgaacccata aaccgttatt ggctatagct
     6301 ccataaaagg catttatctg taaaggtgtt gttagcactt ctccctggcc aattgccaaa
     6361 ttaactttgt gtcctataat accttcctgc ttgccaaagg tctttctgta ccattcggta
     6421 ttggggaaaa aaccctgcct ttcattaggc aaatcaatcc ctgttcttcc atacaaatgt
     6481 gaatcgcgca catagttata aaaatcgtcc aaatttatct tcaacgacaa atcgtaaaaa
     6541 tatgtatcac aggataccct taaagcgtca acaatactgg tactaccatg cccactgcta
     6601 taccagcaac ggaaaaaacg gttgccaact ttaagtcctc cagcacaaaa agccaaacgc
     6661 gtttccggtg taaccaaacc cctgtccaaa ccgattcctg cggttacagg tttaaaaacg
     6721 gaacccggag gataagttcc gtagatgatt ctatccatca ttggcttttc tgcacgattc
     6781 agatattccc aggtttcggg cgaaattttt tgcataaaga gattaggatc ataatccggc
     6841 ttgctaacat aagcaagaat acctccgctg cgaatgtcgc ttacaacaat tgctcctttc
     6901 attccttcgg gaaaaatacc ggcaacaaat tcctgcagat cattatcaat agtcaacaca
     6961 agagagagcc cattcaaggg ttctatatat cccccgtctt caaaaagctg taaactgcgt
     7021 ccttgagcat ctacctgaac aatttccctg ccgtcttttc cgcgtaaaag aacttcgtaa
     7081 tagcgttcca gacctgtttt tccgatatag ctattgaggg aataatcctc tttctgataa
     7141 agttcatatt cttgttcgtt aatccttcct acataaccgg taaagtgatt aggataaaga
     7201 taattgcggg tagagccaat gcgaaaggaa agctccggat aataattcag ttcttcactt
     7261 aaagtcagca ctgtttcata ggggatatta tctgccagca agatttcttc gtaggtctta
     7321 aacctctgtt taaagatgag gtctttcaat tcattttcag aaatgccgaa attgtgttcc
     7381 agaaatttgc ttaaagcagg taaattcttt atcttgccgc ttgttaaaaa gagattttga
     7441 gaaggcacat tttgcactat aggtctatat ttatggtcat aaatttctcc ccgggtggca
     7501 gttattcttc taatacggac aaagttgctt tccgcaatgc gttgatattt agtcccttca
     7561 acaacctgca aacgaaaaag agatacagcc aaaagaagaa aaagaattgc cagaacaagg
     7621 gaaaagatta ctgtcgtttg atttttaatc attgctgatg tttaaacgga ctttgcttaa
     7681 aagttgcaaa ccccaaaaaa caattaaaga taacaaaaca ttatagccaa aaccaagtag
     7741 tgattttgtt aaaagcggac tggcaaaact gaaagttacc ccgaaaacaa gcaattgaat
     7801 aatagagaaa agcagattga caattccaat agtgagcatc ttggctacaa aactttccgt
     7861 ctcaaagggc ttacgaaatt cattgatagc taaagccaac agacaaaata caaaggcata
     7921 caagccaaaa gtcatcggat ttaccgaatc aaacatcagt ccaataataa aaaccacaat
     7981 caaagcaagg tctcgcggac gagtccaaac cgtataaatc aaccaaggta aaagaatatt
     8041 aggaataaca ttccagatag ctaaagcagg cattaccaga atttggagat acaaaaagaa
     8101 caaacccaga ataaaactcc aaattccttt ccaaaccatt agttatccac tctacgcacg
     8161 atgcaaaaag gagttaattc gtcaccctga ccttgaggat tatctttcat tactttttcc
     8221 ataaagtgtt tgctgacatt tgggctactg ccaatcatcc aacaaccacc aatatcatta
     8281 atatccataa cggcaaattc atagccaaaa cgggaatgta agtcctcaca gacctctttg
     8341 ggatgcacag gacctttaat aaccatatcc gtgtaaggtg gaacaggaga agtagttgca
     8401 gcatcaatta aagcggcttg tatacctgca actctgtaaa aatcaccctt cctgcccaaa
     8461 agtttaccta ttgctccaat aaaagctgcc aaaagaattc tgagaacacc tgtttcttca
     8521 atagcgcatt gcatactggt tggagctcct aaaccaatgc cataaggaac tttggcaaca
     8581 aagcgactaa gcaattttgc taaaggagaa acttttattt cggcaactgg aatagcacgt
     8641 ccctgagtaa tagccaaagg gctttcactg atagttaaaa tatctcctgg ttgcattaaa
     8701 ggcaaggcat actctttaat aacttcacca atatcatcct gaggagttag gattttggtt
     8761 ttaacaggaa tgcgttcaaa cttcactcct ccaattgtga taatgggctc tgtttgttta
     8821 cttttactca tttttaactg cctttttcaa gataaagata tgttccaggt tttccaccaa
     8881 agtaaaggga ttgatatctg cactgataaa taaattatcc tgcgattccc gaatgcgttt
     8941 tattgttcca acgggatagg atttgggaaa aagacgcgaa agattggaag ttacaattgt
     9001 atcaccaact gcaatatccg aaccaagctt aattaaattc atagcaattg agccactaat
     9061 atccgcttgt aaaattccct ggacggaagt gcgggaatcc attacaggca attgaaaacg
     9121 gggattggaa aaaggaagaa caatagaatg gtctgacgaa acctgaatta ttttacccac
     9181 aataccatta gcagaaataa ctggatcatc aatttgaatt ccggagccaa ttcctttatc
     9241 cacaatcaga ttaagttcct gatattgacc tgaaatgccg ataatttctg cctgcaccaa
     9301 atccgcttca ccaatatcaa aagctatgga aaaagcactt gtatactgct tcaattcatt
     9361 ttgcagagct aaattttcca gagtggtttc tgctaatctt ttacgcaggg aaaaaacctc
     9421 ctgtttcaat tcctgcctgg tttccactgc tttcaaagac cttacaaacg gaaagaaaac
     9481 cgtattaccc atccagccgg cttttttaag gcgactttca ttgctgccaa ttagcattaa
     9541 cagcgaaagg actattaaaa ttaggggaac aaggttcttc ttcagcatct tatatcccta
     9601 ttaaaatagg gtattatctt tttactaagc ttatcctgaa gaggaaaatt cgggttcaat
     9661 cctcaattct tttaataagc acttgacggt aacggtctat atcatccaaa acctttccag
     9721 cacctttaac aacgcattct aaggcatcag gaacaacatg gacaggtaaa tcaactgtct
     9781 tgcgtatttt ttcatccagc ccttttaaca gagcacctcc accggttaaa aatattcccc
     9841 gttcagcaat atcggcagaa agctcgggag cagttctttc aaagaggcgt tttatggcat
     9901 caataattgt agctactgtt tcagagattg cttcccgaat ttcttcagaa gttatcttaa
     9961 tcgttacggg gtaaccggat acaatatctc ttccgcgcac atccattgtt aattcctgtt
    10021 tcaaaggata ggcactgcca attgtggtct taattttttc tgcggtctgc acacctacat
    10081 aaagattact ttttttccga agatagttta taatgtcgtt atcaatttta tctccgccta
    10141 cccggataga attttgaaca actatgtgcg ataaggaaat aacggcaatt tcactagtgc
    10201 cacctccaat atccataatc atatttccgc aagccatatc tattggtaag tcagcaccaa
    10261 tagctgcagc aataggttca gaaattaaat gaacttcacg cgaaccagca tgcaaagcac
    10321 tgtcgcgcac agctcttttt tctacttccg ttattcctga aggaacacaa acaatcactc
    10381 ggggacggac taaaaggcgt ttcttttgtg cccttaaaat aagattgcgt aacatcagtt
    10441 ctgtcacttg aaaatcggca attacaccat ctttcaaagg tttgataact ctcacctcat
    10501 ccggattttt acccagcata agtttcgccg gattgcctat agcaataatc tttttgttgt
    10561 cagttgaaac agcaactacc gagggttcgt tgatcacaat tccaccattt ttcttataga
    10621 ccaaagtatt ggctgttccc aaatcaattg caatgtcatt agccatcatt ccaaaaaaat
    10681 caaatacgga cattcaagac ctctctatta tctgctggca agcaggatga attttaataa
    10741 taacaatttt gctatcttat accttatttt ttatttagtt ggctatgtca agtaaaaacg
    10801 atttgttacc tctcatagat tttacagaag tggaatctca cagatttcac tgatttcaca
    10861 gattggaaag aagagcatac agattttgcc gatttaagca gattttttta caaagagaaa
    10921 agagaatttt ttttgtagca ccgaaaacac ctaaaacata gtgaaaattt ccaatataac
    10981 tttcagattg aaaatgcata aatatatctt atcttctctg gatgcaggaa tggcaaagaa
    11041 catttctttc aagatatgat ataatttgcc cccaaacttc ttgaccttag taagtcaaga
    11101 gtcctcactg ccaaaataac tttcacacct tttattaaca catttggcag ttaggactaa
    11161 taacgacgat tagacctcgg ataccctttt acttctctcc actaatttta gaattcttac
    11221 cccatttgca atcggtttca ttcaaataga tcgttccccc tttgtgaact tgaatttgga
    11281 cattatctcc aatttcaact tcaccgaaga tattcagttt ggcacctttt ttcaccgtta
    11341 ttttgctgtt atctttaatt atcaatttgc agtccgactt aacggttaca tccgcttttt
    11401 tctccagggt tatatttcct tgtaagatag ccagatggtt catttccgct ttcccttcta
    11461 cttttttggg cagggttgca cacatttgca gtaggcgtcc atcaccagga tcaatactaa
    11521 tttccggtgt attggaatcc gaataaaaca gttcctcaga aaatggatcg tagagagcta
    11581 catattttcc caaaatttct ttagcgctgt tatctatact aaatttaaca gtttgaggtg
    11641 gtgctggcag aaaatggtca tccagtttat cgggaggaac attcagcaaa ctatctgagc
    11701 gagttgggtt attgaccata gaaatatatt ccgttctgcg gttgaccaac ataaaatagg
    11761 gatatttacc ttctgcttta aagagaccac attgcacata gccatcgtaa agatcaacat
    11821 cattttgcac aagtttttga ggtagaactt caattgcctt taccatcaca gatttcagtt
    11881 gttcaggatt tctatatccg gtagttagaa tggcatctgc atcaagccat tcccggggtt
    11941 caaggtaatc agcataaaaa attatttttt gatttgcttc ttttaagccc tcccaacctg
    12001 ggcggaaggt tacttttccc gagggcgaaa catctatagt agtaaattcc tggaaaccga
    12061 gtttcattac attttcttca taaatccggt tataaattct gtaggttaaa attccatctg
    12121 ctccatagca tagaggcaaa agctgtaaac atttctgcat atatttggga ggacgaagta
    12181 aagcccaata accattttcg gcatccgtat aattccattc tccgtatgac tgcggacagg
    12241 gcatatattt agctcctgtt ttcatacaga gttctttata atgtttatat tggtctaaca
    12301 tatcataatc cagccgtctt tgaactcctc ttctttctgt agggttattc cattgcatcc
    12361 atccccagag aggatatata tcaaaagcaa tcaattcggg ctccactatt ttagaataca
    12421 acccgatatg atcatacatc atattgttcg gtttaataac atttttccgg tgcaaaatgg
    12481 agcaggtata aaagcccgaa caactgggag caaaggtttt aaggtctttg aaaatatctt
    12541 tttccagtcg gcgaaaagaa ctgaattgtc cttgagtggg ttcatcggtt aaatagaaat
    12601 actttatatt gtcataacgg gaacaaagat tatacattct gtttctgaca gcttcaatca
    12661 gttccgtatc tgtcttctgg cgtttataca ttgtatcttc aaactccaca taatcaagct
    12721 ctaaaatgcc tttcccgttc caataaatct gaggattgag gttaatcagc aaatatttcc
    12781 atattatatt gctatctaaa agccctgcat aataaaggtc ttgcagagat attttaagca
    12841 caatttcctt atgttgccaa acagaattgc cttcctgatc tttagtgatt aagggaagtt
    12901 tattatattc gcctacagta agataagaaa ccgtgccata tctggaatta taagtattat
    12961 aatggcgcac ctgacattct ttatttgcct tatcaaagcc gttgaaagaa aatcgcatca
    13021 gttccgtatt aggatcatca ggtatatttg aacatttaaa tgcaatagta atatataaag
    13081 tatcatcgcc tgccggatta tctttaatat cccctttcct gccatcaatc atttcacgct
    13141 gtatgaatct gaattcatta cctatattat attttgtata acccttttcc tgccaccgga
    13201 attccagttt gttaaaggca taacctggct gtccgttttt taattgcaaa aggtattttc
    13261 cggaagcaag ctcattaaaa acaggagtgc ctgtagtgta ggaattgtta taaaaataca
    13321 tatcatccag cagattttct ctaaaagtgg aatcatattc tgcttcaaaa cgccagtagt
    13381 tagcaatgga aaatgcttct gaaccgtatt ccggattttt acttccttcc gtaaatgcca
    13441 tatcataaag caaaacatcc aacttatgtt tatccatttc ctttagtaaa aggtcagacc
    13501 tttgaggaaa atcacccggc aaaaaagtta tcacagaagc attataatat gccgtttgca
    13561 ttgaatcaag caaagtataa tagaaatcgg gaatattctg atggcatcta aaataggaat
    13621 aattcgccag taaatactta tcggaatgct taccataaat aggactgagc attattaaca
    13681 gtagggcaaa aatcaaaaat cttatgcgtc gcatttcaac ctcgttaata gcttattatt
    13741 tatctgtttt tcaaacacat aaacatctta tacaggatac agatttagga aatagctgtc
    13801 aagagattat tctgcgttct ttgcataacc acaaggaata taaatagtta ttgtctttag
    13861 ttacctcttt ttttctcttt cctttgatgc ccctcatcat ctttccatca tccacccatc
    13921 ctaaagtatg ggttgatgat gggaaattaa taggtaacaa gaaagcagga gaaaaaggaa
    13981 aaaataaaca aatgttttag gaagctatga tttcttttta tagtgaaaag tcatttgcta
    14041 ttcctgtgtt ctattgtcat tcaaattgat atcttattta gaaacctgtt gtttctttgg
    14101 caaattggaa tttgccaacc ccctttgttt tctacagcat tattagggtt ttattataaa
    14161 attatctttg gggtaaatcc agtaaatccg gttgatcctg ttatctgcgt tctattattc
    14221 ttattcttaa ttctgaatta tttctctttt ctctttgtga aaccaattct ctttttcaag
    14281 caggatatcc aagtttgcgg aaaagttggg aaacgcttat cagttcaatt tccatattat
    14341 ctatctgttt caggaatttt tgtaaagcgt ttagtttatc gcgattatgg caatgggtaa
    14401 taattacaac cggctcattt ctgcccttgt attttgctaa cccctcaatt ttgctgataa
    14461 ttgtagcgtc gctattatcc ggaacatcca gaaagatatc tcttttggcg attttcaatc
    14521 ccaggttctt gccagctgta aaagctgcgc ttttattggt tgtagcgcta tccagaaaat
    14581 ataaaccaag attatccagt tcttccaaaa taatattcat tgtagcaaga tcggaagtaa
    14641 cctcactgcc catatgatta ttggcggcaa cagcattagg aatttgagca taaaaatcct
    14701 gtaataaatc ggaaatagca tatttatcca tccctgtccg gatatatctt ttcccaggat
    14761 tagcttgatg atcaagtgcc tgcattggaa tatgaatcaa tgtttcgtga tttgttttct
    14821 ctgctaaacg agcaactgtt tgcgtatagg gcaaatccgg aagaatagaa aaggcaattt
    14881 ctttcgggag agcggaaaaa tcatcaagta actgacctgc gttttggcca aaatcatcaa
    14941 taataatgac aactggaggc atagcatctg tattattaga ccaggtatat ttataggact
    15001 ctatagctgt catatccagc gcagaaacgg atttgctctc tgttttttct tctgtttttt
    15061 ccatctgttc aggttcaact ttatcttccg gaatttctgt atccgttttt tttgtgcaac
    15121 tgaacaaaag taagcccaag agcaaatata tgattaggat tgtcttttcc actttatttt
    15181 atctccggtt ttaatattaa atttcttcac tgttccggcg ttcacttcca aaacatactt
    15241 atgcagagaa ctgggtgtga ttctattttc actgaaagga attgcctttt catagatata
    15301 attgatagtg ccatcggaag ctataaacag catatctaaa ggcagataag tatcctgcat
    15361 ccaaaaatca tagatatcag gaaattcaaa aataaacagc ataccttgat tttcagccat
    15421 tgtttcacga tattttaagc cccgcatcag ttcttcttct ttttctgcaa tttctatggc
    15481 aaaagtggct tttgtaattc cttcgggaga aattatttcc aaagttccat cctgccgaaa
    15541 ttcatatttg ggtgttggct caggagttgg tgatgcggtt tttttacagc tgaacagaaa
    15601 acaaatcccg atcagcccta aaattataag aattcgcttg ggcataaata cctttcttat
    15661 ttggcaagca acattttacg ggtttgttga taatcgggag tacttaaacg atataaatat
    15721 actccggagc taacttcttt tcctgtatca tccaagccgt tccagacaaa actgtgtttt
    15781 cctgaaggca gaatacagga aaccaaatga cgcacaagct gtcctttaag attataaata
    15841 ttcaacgcaa ccagggtatt attttttgcc agcataaagg acaaagtcgt ttccggattg
    15901 aaaggattgg gactattggg caaaagataa gttactgttt caggagcatt aggatcatct
    15961 gcagcattac cggtatttac aaattgcgaa cctggcactc cgtttaccgt ataatagaga
    16021 gtaaaatcct ggggattagg ttctaaagtt atagatattg attgctgttc aatttcataa
    16081 ccagcataac cataagtgcc tgtttcataa tttattgcct gcacaaatgg ctggttggtg
    16141 taaattccca aatgatgaac ctgattgata taattgggtg ctacagaaac agttagggac
    16201 ttcagttcat ctatattgct gaaattcagg tttaggttat tattatagct tgctgttaaa
    16261 acaggaacag gcacttcttc cggaattcct gtttgcacag gtaaagaata atcagggaca
    16321 taaatatatt tacagagcag atagctgtca aaagcatcaa ttgtgccatt gccatctaca
    16381 tcggcattcg ttatccgata tgcttcccag ggtcttaaat caatttctgg aatgggatca
    16441 agaccaatgg catattcatt gataaaggca atatcctgca tattgattat accctctgaa
    16501 gttacatctc cataaagggc aatattatta ttgatcccta taatataaac atcatcaata
    16561 taaatcccat cggcagtttg accccaatca gtttttattc tgaatctaag gtataattgc
    16621 cctcctgcaa aagaggcaag agaatagctt tgatttatcc agttgtttac ggttcccgta
    16681 aaactatcta aaataatcca gttgcttcca ttggcagatg cttctacata tacaaaatcg
    16741 tagctatttt ccagcgcata tttacacttg aaagacaaat aaggattaga cactcttata
    16801 agcgatatcg gatttgtaag agttatgcta ttgttttgca tattaccata atttcccgtt
    16861 ggagaatcgg ttaatacatt agaatcattg taagttacaa ttccccaagt tcctgttgca
    16921 gtccagtttc ccagtccgtt ttcaaagtta tcagagaaaa gcgaattctg cataataaag
    16981 atattgcgtt tgtcattttg cctgatttgg acaggataag tgtatgttgc attatccaag
    17041 gcagaaatat caatttgata gtcaccttca tacacattaa aggaaaataa tccttcaata
    17101 tcggactgga cagtaattgg cggattggtt tttaaagtaa tgcttgctcc acataatgta
    17161 tttcctgccg gagtacgaat ctgtccctca aaagttgtta gcatagctgg ttgcaaagta
    17221 aagttttgcg taatataacc agttgtggga actgtaatat tcgctgtttg aggtatataa
    17281 ccatcagcag aagcagttat ttgataggtt cctggaagca gtaaacgatg ataatctcct
    17341 attggcaggt ctgtatgttc cagtttggaa tttcctgcta cagtaatgat agccgaaatt
    17401 ggagttcctg aagagttggt aacaattcct ttaacaccgt tttgggcaaa ctcaatatat
    17461 tttaacattg attctctgtt ttcattccaa aaagtatcca acgtagaagc aggaggccat
    17521 ttattattgc taatttcggc agtcatttca atacaatcgg tatagtgata attccaatct
    17581 tgcatactac cagtaacaat ataccaggca gctccattaa ctattccgtg taaaaactcg
    17641 gtgctgttat acattggcag attttcgcga gaataggtta aagccatttc aatgagcaaa
    17701 tcattatccg gagttaaagt agtagtgtaa tcccagggat agtttataac aagggaacct
    17761 ccatgaaaat tgagagccaa atcaaaatcg tgagcgttac tgaaatccat tactgcaatt
    17821 gtttcagcag cccagggttc tccatccgga tgttgaactc ccgtaggcat aggaaaattt
    17881 ctgttcaaat caattccatt agcattatat cgtattcctg ctgcatagcc atctggatta
    17941 agcatcggat ttatccaaat ctctgtgtta tttaccatat tggttatccg cgggtcaatt
    18001 ccatattgag ttgtaagtaa ttgaattaaa cggataagca tatcgtagcc gacaacttca
    18061 tctccatgaa tactgccgaa atatttaagc tccggttcgt tttcttcaat cgttgcatta
    18121 tcacttattt tcaacataag taaaggtctg ttttgaacac tggtgccaaa ttgaaccaat
    18181 tgacagatat tgggatactg atctgccgtc tgttgcataa aattctggta ttcggttaaa
    18241 gtataataac ttctaccatc tttaatatcc tctgccaaag aagcaaaata ttcttctgcc
    18301 ggattgggta aaagctctgt aagtattcct gcactatgaa ttttatccct ttccgcatca
    18361 ttacgcactt ctatgtgaat aatacctgtt tgcaggttta cataatcaat gctgactctt
    18421 agttcattaa gctttttaat atcctcttgc aagttccagc tctgaatacg taaaggatat
    18481 gccaaaacag ccgtggctaa aattagcagg cataaagtaa aaaataccct tttcattctc
    18541 ttatccttat aacttcattg ggaaaaaata attcctttgt cattcctacg caagtaggaa
    18601 tctatatctt actaaataag cataaattat accatttttg tttcaatata gagaaaaagc
    18661 caggcaaaca atgtcacata actggtaaag cttaatgctg cttcattcct gatctgacat
    18721 agttcacttg cagttattgc attgcgcctg gcaaattcag agatttttct ctcgcttatt
    18781 ttgtcaatca aaaacgcaat ttcagggtag tggacaaacg ccaatccgct gattcagaac
    18841 attgtttaac acatctaaaa aacttattga tgctgaagtt atgtaaactt aggggctcaa
    18901 tctcttcaaa atcatcaata aacggagaaa gcttgaattc caacaacgaa ttattattaa
    18961 aaagccaggc ggaattaaga ttaaaagtca attcctgcca ggttccccaa gaatattgag
    19021 gtgaaaaact aaagtaaaaa gtccgaatgt cataacggaa atcaatatta gcagtccagc
    19081 gtctgttttt ggtttgcaat tcataaaatt cctttctctt ttgagcatat tgattttctt
    19141 cataagcaaa accaagatca atcataaagg cattatcggg actattccga aatggcagtt
    19201 ttagccaacg cgggtctaaa gaacgagcta ttaaaggata agtatcaaaa accaggtcat
    19261 attgataact caactgacga taaagagtat tgcttttacc cgttttatag atgtaattac
    19321 tataatccgc tcttatttta tatacctgaa aaaaagctaa acggtctccc agcaaaatgt
    19381 tacattgggg agatagcgtc cagctgttaa tccttttatt attcgcagaa agcagggaat
    19441 ctaaatatac atctttgctg aagccatagc caaaccagtt tcccaaaaga atgcgttcgt
    19501 gccaataatg tttccatccc caaagcaagt tatgggatag caggtcatta tcccattgat
    19561 tgttgtcttc cggaaactta attatttgca aatcaatagc ataatcaaca attaaagaat
    19621 caccgtcact atattcccaa ttaacacgac taccaaaatg cctgttttcc gtatgccttg
    19681 tattaaggcc ataataatag tccttaatcg cgtaagtatg ccttaaatta ttttgccaaa
    19741 ccaaacttgg aaatagctga tagtcaaatt gcagttgagc cagattataa taatctgtgc
    19801 tattacgaat ttcattttgg ctgtaactgg tttttctttg actatagtca tcccttaatt
    19861 gaatttgaac attgtcatca ggataatatt gaactgatcc tgcaaaggtt aaatttctgc
    19921 gtaactgatt atccgacagc gaataattgc tcctattctc attttgtctc ggagcagtta
    19981 aaatgtaaat atcttcgctt ctgtagctgt aattggcaag gcaatccaca ttcaaataat
    20041 taccgtaaaa attgtagtta gcattcactt gggcaaaatc aaaggactcc caatccattt
    20101 ttttgcgttc gccagttgtt cctataccgg ctatggaatt ggaaaaataa gtggcataat
    20161 gaatatcgga accaatccaa taaccctcac tggaaagcaa atttccgcta acatagcgat
    20221 cttgttcatt gcggaatatg cctttgctga aaatacctat atttaaactc tccaccggcg
    20281 tatagttaat ttgataaccc aaagtggcag ttttatttac ataagccgag ggctcaagat
    20341 cactggcatc ataaaaggtt aaatatcccg agctgagagt gtgcaaaaac ttcttccgag
    20401 ctatttgaaa accgacattc aaatccgcat ttttggcttc ctgattgaaa ttcagccggt
    20461 tttcaagatt acttttactg ctgattatta aatttgtgcc ggcagtagga aaatatttgt
    20521 attgcatata attttcaata cttaatgctt gctgactgga atatattgtc caatccagtc
    20581 cctgagtttg agctgctaaa aaaggactat aaatcagcca aacaatcact aagcagaata
    20641 aaatcctctt ctgcaagttc ttctcctctt ttgccaaaat ctatatgcga aagctttgcc
    20701 aattcctctg ttttttcttt tcccaaaaca ggaatcaaat tattagccag tgtttttctc
    20761 cgatgaacaa aagccgcgtg caaaagttta tgaaaatgtt caggatgctt aataactggt
    20821 ttattttctc gcggaagcag caaaataacc gcagaatcca ctttaggcat tgggctaaac
    20881 ttttctctgc taacctgtat tttgctggaa atatcaaaaa ccaaacgcag acggatagtt
    20941 aagggagcat aatttttatt tccaggttta gcactaagcc gttctgcaac ttctttttgc
    21001 accatcaata caattctgga aaagaactgg gaatattctt ccaaaagagc taaaagcgga
    21061 ctggttatct gatagggaat attggctatt aatttgagcg gagttccatc tttccgtata
    21121 agagaattcc agtccgcttg cagaatatcc gtttgttcca atattattct gttttgatag
    21181 cgttcggaaa ggtatttata caatcgcctg tccagttcaa aagctcttaa ctgaacattg
    21241 tttttgataa tttcgtcagt taaaatacct gttccgggac ctatttccca aacgctatct
    21301 tccggttgta attcacccaa ggcaacaatc ttttccgcta tagatttatc ggtaagaaaa
    21361 ttctgcccga gtgctttcag ggctttcata caaaagagtg ctttcggtaa ataaaaggta
    21421 ttttcccggc aagcgcagaa tttaattacc taaccagcta atttttcagc agctatacct
    21481 ttacagcttt tgcagacctt gtaggtattt tccccaactt taacctcata gagaagtttt
    21541 atacccgttc tatggcagac gggacattct cctctgccgt tattaggaac ttttttcagt
    21601 ccttttccgc gattattttt tgccattgtt ttctcctttt tctgtttatt attttataat
    21661 tctatgcttt tcacctttaa ctaaagtaat cacgagtcct cattgccaaa taaaattgaa
    21721 ggattgtgaa attattaaag gcagtgacga ctccttactt tttaagtggt taacaaagtt
    21781 catcaacatt ctggtttttt agcagttatt tgcttcctca tcggcaaagg gatttagcgc
    21841 ttttttgatt atcggttttt catggggaat caaacagaga aacttcaatg tttcatttcc
    21901 tgtatttttg aatgaatgta aaatatcagg atcaacgaaa attgcgtcat cagctccaaa
    21961 agccatttct ccccggtctg taactaaggc accttttccg gaaagacagt aaacttcatg
    22021 ttcccactta tgttgatggt ggggagtatg cccaccaggt tcaatttcaa acatcctcat
    22081 tgcaaaagtt ggagcgccat ctttttgggc aatcagccag cgaatttgtg ctttttcagc
    22141 accttccgca gttacgggtt ccaattctac ttcatcgtaa tggataattt tcattgtttc
    22201 ctccttctcg gaatttattt tgtaatatca tttttgcctt aaaagtcctt tggcattcct
    22261 gcaaaagcaa gaatcaaaat ataatttact atcctaactg catttccaat ataaaaatta
    22321 tacaatagat aaactaaagc gccaagaact aaagacaaat tcttaaatat gatagacagg
    22381 gtaagcagta actacaatat cgttttcaat tttccggcag cttgttttcc atatatacaa
    22441 tgctttatag atactttcaa tgcccaaatt aggcaaagga cttttagttc cgcctaaaat
    22501 tttggctccg tagataaaga taattttatc tatcaattcc tctttgagaa atgcttccgc
    22561 tattccgctg cctgtttcca gcagaacgct gtaataattc attttgtata ataaatttaa
    22621 gacctcgtgt aagttaaagt gtccgtcttt agtttccaga gcttcaattt ttaccccttt
    22681 ttgtcttaaa atatcggctt tatctgtttg cagattatca ggcgaggtaa taatcattcc
    22741 gggaaattta gcagagatac ttacaaaata ggaaagcaca ggtattttaa gataagggtc
    22801 taaaacaact ctcagtggtt gtttgtaatt tcccgggagc cgcacattca gtcgtggatc
    22861 atcaaaaagg atagtcctta ttccggttaa aaccacatcg ttttcactgc gtaattgatg
    22921 ggcatattcc cgtgcttttt ttccagttat ccagtgtgcc gtgccatctt cagcggcata
    22981 ttttccatcc agcgtaaggg ctgttttcaa agttacgaag ggtctttctt tctgtatata
    23041 acaaaggtaa cattccagct gggttcttat tttttcttcc aaaaagccgt aatgaacatc
    23101 aattcccgcc tctttcaaca tcgcaatccc tttgccttta accaaaggat tgggatcata
    23161 aattccaata tagacctctt taattccggc atcaattata gcttgagcac agggcggtgt
    23221 tttaccgtaa tgggaacagg gttctaaagt tacaaataaa gtagcgtctt tggctttctt
    23281 gcctgctttt ttaagtgcct gaatttctga atgatcgctt ccatattcca aagtgcagcc
    23341 ctcacttatc acctgattat ttttcacgat aacagaaccc acaaagggat tgggagaaca
    23401 tttacctctg gctttttctg caactttaat tgctcgttgc atatattgtt ttatttgggc
    23461 atcggtcatt gcagtttctc caaatttttc ctggcatatt caaaagcggg atatttttgc
    23521 acgatttcct gccaaagttc ttttgctttt tccgtttctc cgtttccggc ataagcagcg
    23581 gcaagattat tcagcacatt ggggtcatcc ggttgtaaaa tcttcagttc ttccagccag
    23641 atttttgcct gttcataatc ccctttcaca aaataatggg cgcttaaatg taaatagggc
    23701 tcggtccagc tattatcaag agcaatggca ttaaagaaat ccaccgccgc ttgctctctt
    23761 tgttttacca attgccagta taaagcgcga tagtaataga gatcgggact atcgggataa
    23821 attttcaagc tgctgtttat cagttcccaa actttagcgt catcagcttt gcccagtgct
    23881 tctgctatag ctgtatatat ctgattttgt ttaaaatatg cttgcggact gcctgcaata
    23941 ttttgtctta ttttccagct gccggttata ttactcagca ccaaagtcca gtcaatttta
    24001 tgatttaatt ccatataaac gagagcgctt attccatcct cacccaatcc gctgtataaa
    24061 atactgtaag tagaaatctt acccagctgg ggaatggctt gaatcagttc tgtatagcgt
    24121 tctgccaaat cctccataga gctctgattg atactatatt gtcgtaattc attccatttc
    24181 agttgaataa gggttttcat ccatttttct acacagttct cagggtcttc ttttttctca
    24241 gcaggggtaa aaggcaaatc taactttttc agcagataat ccattggaaa atataccgga
    24301 tactgaaatc cactcaacca ggcaagcatt tcttgggggt tttcactagc ttctttagcg
    24361 ggagttttat ttttcaagaa aggattttct ctacccatcc aaaggtctat atggcatttt
    24421 atagcatttt ctgcttcggc gcgagaatca gctttaaagg aaggaaacgg ttttgattct
    24481 tctttttgag gagcataatc acaattttcc ggacagcgtg tatcacagcg tgtttcatta
    24541 cagcattgcc agcaaatgtt ttttcttttg cgaggacata ctctaattgc ccgttccgta
    24601 taacaggatt gacaacgcga gcttttgaat aatttaagca tttactgcct ctctaaaaaa
    24661 ggaggcattt tacgcttgaa acttgaattg tccatcattt tctgcacttt aacgattatt
    24721 tcttccgcta cttccggttt tctttctcct tccgttaaat atttcaggac gctatctaaa
    24781 ataggataac tgattcccag ctcggattca tcgctttgcc ctgaccataa atcggctgta
    24841 ggggttttat tgataacttt ttcgggcaag cataattccc ttgccagttc ccaaacctca
    24901 gttttgtaaa gatgtgctat tggttcaaaa gcacaagcag aatctccgaa ttgagtgaaa
    24961 taaccgacta aaagttcgct tctattgctc gttccgatta ctaaagcgtt gtgttttgcc
    25021 gcctggtcat aaaggatatt cattcggatt cgtgccatcc aattcccttt gcgtaaagga
    25081 cttgcctccg gagcatattt tttaaaataa ctctccgtaa gttcatctat atttatggtt
    25141 tgcataaata gacctaagca ttctgccact aaaaaagcat ctttaacact ttccggattg
    25201 ctttcccggg aaggtaaaca aaaggcaaat aaatgctctc tgccaattgc ttgaacagct
    25261 aaagcggata ccagagctga atccacgcca ccggaaagac ctatcacgag attttcaaat
    25321 ctgcttttcc ggcagtaatc ccgaataaaa ttgcttatcc tctctctttc ttttttataa
    25381 ttcattatga ttttcgcttc cttccattta tctattactt ttttatcatt atcttttttt
    25441 ctcttttttc cgtcaagcaa attctctttt ggtgttattt ttctaactta acataacaaa
    25501 ttgccctaca aacaattgct acttcttcct taatagcatt gcttacccat tgcttaccca
    25561 ttacggatgg cattagcaat gagtccgtat tgctaagaca atgctgttgg gaaagggaaa
    25621 aatgaaaaaa gaatgatgtg agagcagaag cagtaagatt atcaaagtga gtttcttcaa
    25681 agtgagatta tcaaaagtgc aattgaaaca tcttatctct tttgttaaga atgagttccg
    25741 taggaacgac agatactaac aacgggtttt aacccggtga aaagtgataa gtgaaacatc
    25801 ttatctcttt tgttaaaaaa aagttccgta ggaacgacag atactaacaa cgggttttaa
    25861 cccggtgaaa aa
//
LOCUS       DAOPFL010000013        25423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1967_length_25423_cov_99.102373, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000013 DAOPFL010000000
VERSION     DAOPFL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 25423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25423
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1967_length_25423_cov_99.102373"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="PLG69_02055"
     CDS             <1..1490
                     /locus_tag="PLG69_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPI42324.1"
                     /translation="GNNNGKLDPGETATLSIPLNNSGGANSPAGSATLTCTTTGITIN
                     NGSASFTAITAGSSATLSFSITAASSVSQGTLAALNFNATAGAYTAAKTQNLEIGAPT
                     VVIIGTGTSTQSYPLDRYYNYSAFEAIYLASELQYIGTIKSLGFYKASGSDVNPIEAV
                     TIYMKNTAESTLSDGDYSTTGYTQVYSGSFTNDATSGWMSVDLSPQFTCNGSNLAILI
                     VKGYQEYISNYPNWTYTTTTTTRVRCNHSDSAAPTSLTGSTSLPNLQIKIFPTPGILY
                     PPQNLTAAPRNGSVTLSWQAPLSGQPTGYKIYKNSALLTTVTGLTYTDTAVVNGTTYS
                     YYLKAVYATGESSSTATVTATPFNVFETGAIIGTGTASTGTTEASPINVFYQSLHGQS
                     VYTKAELNNAGVFGPVYISQVGFNVTGVPNKTMPNFVVRMKHTTATDVSSWIDNTNLV
                     TVYTNASYTISTTGYNMLTLSTPFLWNGTDNLLIDTAFGLIGSYS"
     gene            1566..3590
                     /locus_tag="PLG69_02060"
     CDS             1566..3590
                     /locus_tag="PLG69_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPI42325.1"
                     /translation="MFSSGSTSVYKPNVKLFLTAVAGNPQISVNPTSFSFGSVAVGTT
                     STQNFTIINTGGSTLTGSIITPTGYSVTAAAKEIRNTLSFSIPAGQSKTYILSFSPVS
                     AISYNGNVTISSNSQTQANLSLPVTGSGYLPPTISVNTNILSATLTSGAESTQTFTIS
                     NTGSQSLTYTMVVSEPTGRNEAISPVKTTGSKSIAGSTLTLNADEYTSGTTVDWTFTV
                     TNASTDTEWLKEIIVTFPAGVTVNSATNFVGGSGGDLVPDNTTGAGITISWFGESDYG
                     YGLIHGSESATATVNVTISAGLVTTIVLPYTINGDIWGAEPNTVSGEISLACSLPPVE
                     WFSVTPNSGNIPAGGNQTISGHFSAVGMAEGIYNAVLTIQSNDPVHPITTLPVTMDVS
                     SGNHSPRITLPESFSFAKNGSLTVAFNSYISDADNDPLTLSVSGNNHIHIAINGTQVT
                     FTADLNWVGSENITFGVSDGELTAYDNVIVIVNPVNVPDWQPIVYPNNPATVYAQVSI
                     EGIPAQLNDLVGAFVNNECRGTGEIVLIDRATAYSTILVNLASDGELVQFKIYSYAND
                     AVYPVQETLTMQTGSVYGSAENPVVLNGTFNIVLTPPQVNLVSHQNTYRLTWNAVPNA
                     NNYRIYSCSEPYGTYQLVHTTANLYWEIPTTQQKCFFKVVAEQIGISKGK"
     gene            3591..5453
                     /locus_tag="PLG69_02065"
     CDS             3591..5453
                     /locus_tag="PLG69_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPI42326.1"
                     /translation="MRKTILVALLLLLAFSLFAKEWNQYYFRFELADKTTLPEISNII
                     SIDNVRGNWVYAYANDEEYSKFRELGLKTQLLPSPASLIEPVMSSDVKQTKLWDSYPT
                     YDSYVNTMNSFAANYPNLCQIVNAGTTVNGRSLLFAKISDNVNIAEAEPEVMYTSTIH
                     GDETTGFVLMLRLIDTLLSSYGTDQRLTNLVNNLEIWICPNTNPDGTYYGGNNSVSGA
                     RRYNYNGYDLNRNFPDPNGNQYSGQPLQQETTLMMNLANNHHFVYSVNFHGGAEVVNY
                     PWDYTYTAHPDENWYISTSFVYANNAIANGPSGYFTSVSSNGITNGADWYVITGGRQD
                     WMNYSAHCREVTIEISNTKMPSASTLPGYWNYNYEAMISYLEQAMYGIHGIVQDPYGN
                     PLSATITVNGYDNSYSTVITDPAKGDFYRYLSPGTYNLTISASGFPDKTISGVVVNAN
                     TATSISVTMGELPHYQQITLSPGWNLLSFNVDLGTNNFSSVFGSNLLQIKDTAKSYAP
                     SMPSYFNTLSSLQSAKGYWVNNSSAQNLSIQGQLLNTSNYPIALNSGWNLIPYLPDNS
                     LPVASAIASILTKTQEVRYLSSVWNPVSGGALSVLEPGKAYWIRVSEPCQLLYP"
     gene            complement(5943..7721)
                     /locus_tag="PLG69_02070"
     CDS             complement(5943..7721)
                     /locus_tag="PLG69_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPI42327.1"
                     /translation="MPGINCLINAQGITQQTQNTIDNYLQRLEATFPHLQFTKLMPCR
                     EVLISISQHRGYRVYTEKYRGWQIFHELQDNSFLTQAFQDILEQAEISQNPLSNMQNR
                     WNSELKEINNSFFLFAVNPELHKIILANDALARLPVYYTKQKEYFLLGRDISFVKAIS
                     NQLTLDPLFLALYLSLTYVPGRGTFYREIDTLAGGTLGVYDWLTDELQISSQQELRFI
                     EPLFGRSHKKWLAELVETFTAICVSYHTELPKVVSLSGGMDSRCVAGALQSKNIDFTA
                     ISFLDANQDAQDDVLIAVQTAELLKKTHRVLQLSPCAPEHYEKLFSLKAGLNYFCVAM
                     FLQYLEMILALYPESALFLTGDGGDKVMRYLLPDKQLTDEKQWLNYWYNQNAVFSPKA
                     SAELFGIQQKAMEEYLLNHISTYPTKDYNYKYAFALLAERSARWAFEGEDRNRYYFRS
                     ETPFLDYQFYRLAMQIPMEQKKDNLLYYSFLSALSPALAKLRYAHHQWSPAKMRNPFY
                     RYLVNKTRNFRIQYRKPKGKINSQPKFAEQEYLITRILKQSENPLLQALMPGAKNILT
                     LPDLQKLSSNQLGTLYTCVSVITGIV"
     gene            7957..8682
                     /locus_tag="PLG69_02075"
     CDS             7957..8682
                     /locus_tag="PLG69_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPI42328.1"
                     /translation="MQFDVSYFCFDGNSGKKNQDRVLVNKDLLTEGINHLQNLDNLFC
                     FVADGIGSLANSENAAQFVLEQLKYLPLKAEQNWQEIIDNFLQDINYQLVLWNRQQGD
                     FFDSATTLCGLIYQEGKFLTVNAGDSEIWLYREKYLQRLNKLNVLWEDIPNSPITNYF
                     GTDYPNLKLDFSSAVSSLNPGDKIVVTTDGLLKAISKKELIQILEEDSPLYIKIDSLY
                     HKLCAKYNPDNLGAIFISEKSNG"
     gene            complement(8940..10181)
                     /locus_tag="PLG69_02080"
     CDS             complement(8940..10181)
                     /locus_tag="PLG69_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI42329.1"
                     /translation="MKKIFIFTLLISAVFCFAFQVDTFGPTADDFSCANLDQVPASFG
                     IQIANQKKLCLWENGIWNSYSYFTTGLTIKGTYMLNADTMLVAMGIYSYSDGIYNFDI
                     NTHNWQLNDWFILPNFVKYNPHNQLYYVGERDGLFYSANGHNWTRITSLGSNKCNSIA
                     WYDSNIITNNGQMVYYSNDNGLNWQQSSISNLSSFRFDDNGILYAIMDVGSDSDGLWR
                     SNDFGANWTNILYTSALSCLGPIYNNNVTLGWRESYADSCFVGMLNSSGEVTFFNHPL
                     LNAPVKQIDIFPYINTPSFYVLNSGGCFFVTGFLVGIDDPVIPQIPQAKISTYPNPAL
                     SYADIYLSKVSPEPCKLTIYNLKGQKVRNLDLAETLKEQTLSWDLKNNNGFKVSAGIY
                     FLVLQDTKGNCLAKTKLAVIK"
     gene            complement(10405..11220)
                     /gene="pyrF"
                     /locus_tag="PLG69_02085"
     CDS             complement(10405..11220)
                     /gene="pyrF"
                     /locus_tag="PLG69_02085"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPI42330.1"
                     /translation="MAKIASKSFWYKYQERYQAIKSVLCVGLDSDFSQLPEGVKNSEN
                     PIWEFNKAIIEATLDYACAYKPNLAFYLADGIRGLDALYKTVEFIPDNLPVILDCKIG
                     DIGNTMNAYLESFFTDLKVDAITLNPLMGSDVMHPVQDRNAFAFVLCLTSNPSASDFL
                     QEGMAEKIANWLQDYPKENFGAVVGATHSANIKTMRNLLKDRILLIPGIGAQGGDLKT
                     VLQEAKDSSETPNILVNSSRGIIFASKGKDFATAAREEARKLTQEMKNALSKG"
     gene            complement(11186..11722)
                     /gene="pyrR"
                     /locus_tag="PLG69_02090"
     CDS             complement(11186..11722)
                     /gene="pyrR"
                     /locus_tag="PLG69_02090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPI42331.1"
                     /translation="MLVKSQIMDKDQMERSLQRMAHEIIEQNRGLEKIRLVGIRSRGV
                     PLAERLSSYLKLISNQEVPVGILDITLYRDDLSTISHQPVIKGSAIDFDIEDAVVVLV
                     DDVLYTGRTVRAAIDALMDFGRPKQIQLAVLIDRGHRELPIKADYVGKNVPTSKEEII
                     KVSLEETDGEDSVKIVLV"
     gene            complement(12047..14032)
                     /locus_tag="PLG69_02095"
     CDS             complement(12047..14032)
                     /locus_tag="PLG69_02095"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HPI42332.1"
                     /translation="MDKHSCVEMTTMHYEPDRPKAENPVKVQDLTFRDGHQSLFATRG
                     RTEDMLNVAELMDQVGFYSMEIWGGATFDTMHRYLGEDPWERIRVLKKHLTKTPLSML
                     LRGQNLVGYQNYADDVVEAFVQRCCDNGIDIFRVFDALNDFRNFITAVKIIKKNNKLF
                     EGAICFSLTEQRMGGDIYNIEYYVAKAKQLEEMGADTICIKDMAGLIAPYDAYELIKA
                     LKENVKVPVHLHTHFTSGMGDLALFKAIEAGVDIIDTCVAPYAYRTSHPAIEPLVISL
                     LGTNRDTGFDIKLLNKIGKEMEKDIPKYLEFADTTKFSIIDTDVIIHQTPGGMLSNLV
                     NQLRQMDALDKLEDVFKEIPKVRKDLGQIPLVTPTSQIVGIQAVNNALFDSYEGEYSR
                     ITEQVKDLCYGLYGKTTLPINPEVQKKALKGYPRGETPITCRPGDVIPPAMEQVHKEC
                     EGLAKNIDDELIVALYNITGKRFLRIKYGLEPMPEEMKPKTLADVKKEQELIAKAKAG
                     KLIEKPEPQEKPQDLRQFDVFVDNTYYLVEVSEKGGTPKIVNSRPAPVISETIVKPAN
                     NPNPQVKPSNPAPEPKPAPVVNSEGGTKITAPMPGMFIRYEKKPGDPVKQGETVLVLE
                     AMKMYNNIPSPVDGVLVEAPFTGGANVAKGDVLAVIK"
     gene            complement(14046..14234)
                     /locus_tag="PLG69_02100"
     CDS             complement(14046..14234)
                     /locus_tag="PLG69_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42333.1"
                     /translation="MKDNKEIAAIGAVIALIQSEETSITYKEPVRGTPSQPYTAYGRE
                     LTMHYRDLTQRRIIKRCR"
     gene            complement(14254..15807)
                     /locus_tag="PLG69_02105"
     CDS             complement(14254..15807)
                     /locus_tag="PLG69_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPI42334.1"
                     /translation="MQSTKEAIQGLLEKEEKILQMGGEKAIAKQKQGGKLTARERLDL
                     LFDEGTFRELDMFVSHRCDNFGMEKIEIPSDGVITGHGLVDGRPVFAFSQDFTSRGGS
                     LGEMHAAKICKIMDMALKSGVPLIGIQDSGGARIQEGVDALKGYGDIFFRNSRASGVI
                     PQITAIMGPCAGGAVYSPAMTDFVFMVKNTSFMFITGPDVIRAVTGEETTQEDLGGAM
                     AHNSKSGNAHFACENDADAIQKIKVLLSYLPSNNMEEPPRIKTNDDPWRLCPELDSII
                     PDDPKMSYDMRDVIHSVVDDGIFFEPHYYYAQNVIIGFARLNGRTIGIVASQPTILAG
                     CLDIDASDKATRFVRFCDAFNIPLVTFVDVPGYLPGTHQEWNGIIRHGAKLLWCYSEA
                     TVPKLTVVTRKDYGGSYIAMSSRHLGADMVFAWPTAEIAVMGAQGAANIIFRKEIAAA
                     EDQEAKRQEMIQIYEQEFNNPYVAATRGYVDAVIVPAETRKRLIDALEILCTKSESLP
                     PKKHGNIPV"
     gene            16300..17757
                     /locus_tag="PLG69_02110"
     CDS             16300..17757
                     /locus_tag="PLG69_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPI42335.1"
                     /translation="MKELSADLSNLAEVFSALFPPEKKSSAQTFFLKQLSLKMHSYYQ
                     GKMQTLPKAGIWGFNWFNVWYTPGVSSVSTAIRDNIAKSYELTNRSNLVAVVSDSTRV
                     LGDGDCGIAGGLGVMEGKAMLMKYLGACDAISLCIDSRAENDKPQAQKIIDFVKMLQP
                     SVGAVNLEDISQPNCYLVLDELRETCSIPVWHDDAQGTACVTLAGLINALKLADKEID
                     NIKCVLYGAGASNTTIAGFLLQSGLNPERLIIFDSKGSLHPGREDLKSNPDKFRQWSL
                     AQNSNPECVEGIENALQNADVLIALSTPGPGTIKPEWISKMAKKSIVFTCANPVPEIY
                     PYNAQKAGAFIVATGRGDFPNQINNSCGFPGILKGVLTVQASKITDTMAIAAAHSLAE
                     YAEKRGIHPENIVPQMDEEDVFAYEAAAVARQAITEGLAGLKFTSEEVFKQVQKEINA
                     TRALCQEMMENGYIAPPPRNLIAETLQQTIVRFPN"
     gene            17761..18657
                     /locus_tag="PLG69_02115"
     CDS             17761..18657
                     /locus_tag="PLG69_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPI42336.1"
                     /translation="MDLSGINNFRKSRTGKIILYFLKLGLSIFILWMIFRKVNFISAL
                     KDIISLPLGIVLLLLVISFIRHTLQFHNWYFSLQINNLFKARKKEVISSYLIGQTLRF
                     AIPGGPASFAKIFYVPNSSKISSFWSSIAEKSFMTWTTWFFALLAGMLYYRQSPLWVW
                     IILLLILIFLPFLVTLIFGASKKFSSLQALYSVQFPKIISVQVLSNLLCFVQYWLILN
                     CFLPINFWQSWIRMALTQFSNTIPITISGLGLREGFAIHFLAGAGFTAEQAVSVSLTL
                     FFIHDVLPALIGIFYLLKSKKV"
     gene            complement(18911..19861)
                     /locus_tag="PLG69_02120"
     CDS             complement(18911..19861)
                     /locus_tag="PLG69_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPI42337.1"
                     /translation="MKLWITYLKAVLAMLFWSATFVWFKIAYKTYLPYEVTFLRLLLA
                     TILLFPFVWLTGKREKVPKKDLLHLMLVSFCEPFMYFIGEANGMRIVSSTLGSLIIST
                     IPIFSAVGAWLILREKLPLLVVLGLILSTSGVAVMSLGSDNFSATFKGILLLLIAVFS
                     GVFYGITVRNLTLRYNPFTIVAWQNFFGMLYFLPLFLVVDGKHFFSVQHSLEGLATIA
                     AMSIFASVIAFILYTGVIRDLGVAKSNIFTNLIPVFTVALAYIILGDKLGLPGWIGLI
                     LAIIGLFLSQYPDWQKYRKQEKTELLPVVENAETNISNEL"
     gene            complement(19861..20460)
                     /locus_tag="PLG69_02125"
     CDS             complement(19861..20460)
                     /locus_tag="PLG69_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPI42338.1"
                     /translation="MHKLTKLNTPIFWAEGHPGSLKRENWQIEVSGSCEKPGIFTWES
                     LLALPQKEVKGRLTSVTRWSVEGFWQGIPLSEILQAVAVKENCRYIRFWSINKIYDTS
                     IPIEIANKEKSLLAYSFDGELLTEDYGGPIRAFIPYLWGYKSAKSVVAIELMDYYVPG
                     FWEKRGYTDSGEIEAGPCRDLNDGGKIKYIPDGEVLKFN"
     gene            21186..21671
                     /locus_tag="PLG69_02130"
     CDS             21186..21671
                     /locus_tag="PLG69_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPI42339.1"
                     /translation="MAIYSVNEVIEMAVQIERSGYAFYNEAVKRKDLDEKAKDFLIYL
                     RDEELRHEKTFLALRNEVDLQVLQLSVDWELIAEYLKTIVESRIFSNEFSAIQLATKA
                     KDIKEIVENAITFEKDTLLYFHTLSDNINESRTHNALRKIINEEVSHILKLSEMKKKL
                     V"
     gene            21811..22341
                     /locus_tag="PLG69_02135"
     CDS             21811..22341
                     /locus_tag="PLG69_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPI42340.1"
                     /translation="MAKGKHRKNKKSNTGRIISILLVLFILLILVYNPISLRLTTIGV
                     AIYYGLNPVVFYRVIKTESSFRSFAISDAQAIGLGQIKESTAFYIHKDHKRGYLFLPV
                     YNLKLAAKYLVYLKNRYNGNWSLTLAAYNWGESNVNRRLKGIEIEKDKDYSSLFRDIP
                     ETYSYITKILYPKNKA"
     gene            22397..23041
                     /locus_tag="PLG69_02140"
     CDS             22397..23041
                     /locus_tag="PLG69_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42341.1"
                     /translation="MLRNQNGISVYTVLSIILFIVLVFILALPNFFNLDKEKNLEDCI
                     NNMKQIWVATTDYMRDTNADFNGDLSLLIKTPKKDDPKNTYLSSNLYCPETSHQKKEY
                     LVYGKYVAEQIGTEIKHNYGIIILCPNLAQYPKHIIEKGFYENMEPTQLQNYMSEDID
                     YINSETGLNGAKKVELINKYIEIWKTDPDAFAKRKANTTALKAILFPEKFGITK"
     gene            23153..24433
                     /locus_tag="PLG69_02145"
     CDS             23153..24433
                     /locus_tag="PLG69_02145"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPI42342.1"
                     /translation="MFYNEFLEHIFKRYSGNVKLELNRMQGLLNDMGNPERKLNGFHI
                     GGTNGKGSVCATLEALCRESGLNTALNTSPHLINYNERFRINGKEPSFETILNTFLRY
                     ENLFDKWEASFFEITTALAFSMFSEQNVDVSIIEVGLGGRLDATNLFVPDVAVITNIG
                     LDHIKTLGGTLEIIAGEKAGIIKEGIPLVLGDIEDSPRSIIEAVAKAKKVPVFRYKRD
                     WQTSIKNDNLTGINFDYSFGNYEFPNLEANLIGEHQAINIGSALTAFILYAEKKKLPV
                     AEEVIRKALQHISWKGRMQVLSQKPIIIIDGAHNVHGVKALMSTLEKIFPNRKVKFMV
                     SILGDKDYKQMLSLICPKAEQIYVARNQSERAASVEDQVAVIEEYNIPYKTAPTVAEA
                     YSLAVKECKEDDILIVCGSLFTVGEVLEIPKDNA"
     gene            24408..25349
                     /gene="rnhC"
                     /locus_tag="PLG69_02150"
     CDS             24408..25349
                     /gene="rnhC"
                     /locus_tag="PLG69_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HPI42343.1"
                     /translation="MKSQKIMHKELENYLSHLLPELQKTGISIDEQIEIPYGVQLKVS
                     RHNYKSILNIYYSQKKGISPVPTGNNNPLKKLLWDLCRLQEELKLDMPPMHTWNAWIG
                     SDECGKGDYFGPLVVCAFAYKRDSENEFKNVGVKDSKKINSEEIKRIAKYLYKNYANC
                     IHCIVIKPEKYNELIAKFKAQKKNLNDLLAWLHSTAIIALQKNHPESQGVLVDQFSVS
                     HKTKYALQEKQFPLPCIERTGAEIDPAVAAASIIARYQFLEAFEAMRKFYQLNFPQGA
                     NGNVIKYAQDFIKQYGEKRLGEVAKLHFKTTNQIENI"
BASE COUNT         7823 a         5005 c         4760 g         7835 t
ORIGIN      
        1 ccggaaacaa taatggtaaa cttgatcccg gagaaacagc aaccttaagc attcctttaa
       61 acaattccgg gggagctaat tctccagctg gttctgcaac tttaacctgc actacaacag
      121 gaataactat caataatggc tctgcatctt tcacagctat tactgccgga agcagtgcta
      181 cattatcttt tagtattact gctgcctctt ctgtcagtca gggaacttta gcagcactta
      241 attttaatgc tacagctggt gcttatacag ctgctaaaac ccaaaatttg gaaattggag
      301 ctccaactgt tgttattatt ggtaccggaa catctaccca gagttatcct ttagatagat
      361 actataatta ttccgctttt gaggcaattt acctcgcttc tgaacttcaa tatatcggta
      421 caatcaagtc ccttggtttt tataaagcca gtggtagtga tgttaatcct attgaagcgg
      481 taaccatcta tatgaaaaat actgctgaat ccactttaag cgatggtgat tattctacca
      541 caggatacac ccaggtttat agtggcagtt ttacaaatga tgctaccagc ggatggatgt
      601 ctgttgattt aagtcctcaa tttacttgta atggttccaa tctggcaatt ttgattgtaa
      661 aaggttatca ggaatatatt tccaattatc ctaactggac ttatactacc acaactacaa
      721 cccgggttcg ttgtaatcat agtgattctg ctgctccaac cagtttgacc ggttctacca
      781 gtttgcctaa cctccaaatt aaaatattcc ccactccagg tattttatac cctcctcaga
      841 atttaaccgc tgctccgaga aatggaagtg ttactcttag ctggcaggca cctctgtcag
      901 gtcaaccaac cggttataaa atatataaaa attcagcact gctaactacg gttacaggtc
      961 taacctatac agatactgca gtagttaatg gcacaaccta cagctattat ctgaaagctg
     1021 tttatgccac tggagaatca agctctactg ccacagttac agctacacct ttcaatgttt
     1081 ttgaaacggg tgctattatt ggaaccggaa ctgccagcac gggaacaacg gaagcatctc
     1141 caatcaatgt tttttatcaa agcttgcatg gtcaatcggt ttatacgaaa gccgaattaa
     1201 ataatgccgg ggtatttggt cctgtttata tctcgcaggt aggatttaat gttactggcg
     1261 tgcccaataa aacaatgccc aattttgtag tgagaatgaa acataccaca gccacagatg
     1321 ttagcagttg gattgataat acgaatttgg ttacggttta taccaacgcc agctatacta
     1381 tttcaacaac cggttacaat atgcttactt taagcactcc ttttctgtgg aacggaacag
     1441 ataacctttt aattgatact gcctttggtt tgataggaag ttacagttaa acagggacag
     1501 tacaatatac ctctgttacc aatggctata gatatacacg tagtgattct gctgatcaaa
     1561 ccaatgtgtt tagcagtggt tcaacctctg tttataaacc taatgtaaaa ttgttcttaa
     1621 ctgccgttgc tggtaaccca caaatatctg taaatccgac ttctttttcg tttggttcgg
     1681 tagcggtggg aacaacttcc acgcagaact tcacgattat aaataccggc ggttctactc
     1741 ttaccggttc tataattact cccaccggtt attccgttac cgctgcagca aaagaaattc
     1801 gcaatactct tagcttttcc attcctgcag gacagagcaa gacctatatt ttaagctttt
     1861 cacctgtctc tgcaatcagc tataatggca atgttactat tagcagcaac agtcagaccc
     1921 aagctaattt atctcttccg gttacaggca gcggatatct tcctcccact atatctgtaa
     1981 ataccaatat tttatctgct actttgactt ctggagctga aagcactcaa actttcacaa
     2041 tcagtaatac cggctcacaa tccttaactt atactatggt agttagtgaa cctactggca
     2101 gaaatgaagc catcagtcct gttaaaacaa ccggcagtaa aagtattgcc ggctctactt
     2161 taactttgaa tgcagatgag tatacttccg gcacaacggt tgactggact ttcacagtaa
     2221 ctaatgccag cacagatact gaatggttga aagaaattat tgtcactttc ccggcgggag
     2281 taactgttaa tagcgcaact aattttgtcg gtggtagcgg aggtgactta gttcctgata
     2341 atacaaccgg agcaggtatt accattagtt ggtttggaga atctgattat ggatatggtt
     2401 taattcacgg ttcagaatct gctaccgcta cagtaaatgt aactatcagt gccggattgg
     2461 ttactacaat tgttcttccc tatacaataa atggcgatat ttggggtgca gaaccgaata
     2521 ctgtaagcgg agaaatttcg cttgcctgtt ctcttcctcc ggtggaatgg tttagtgtaa
     2581 ctcccaatag cggaaatatt cctgccggtg gaaatcaaac tattagcggt catttttctg
     2641 ctgtcggaat ggcagaaggt atttataatg cagttttaac tattcaaagc aacgatcctg
     2701 ttcatcctat aacaaccctg ccggtaacaa tggatgtttc ttctggcaat cattccccgc
     2761 gaataactct gccggagagc ttcagttttg ctaaaaacgg ttctctaaca gtggctttta
     2821 attcctacat cagtgatgcc gataatgatc ccttaaccct ttctgtgagc ggaaataatc
     2881 atattcatat tgctattaac ggaactcagg taactttcac tgcggattta aactgggtgg
     2941 gaagtgaaaa tattactttt ggtgttagtg atggtgaact aactgcttac gataatgtaa
     3001 tagtaatagt taatcctgtt aatgttcctg attggcaacc tattgtttat cctaataatc
     3061 ctgctacggt atatgcccaa gtgtctattg aaggcattcc ggctcagctg aatgatttgg
     3121 tgggtgcttt tgtgaataat gaatgtagag gcacaggaga aattgtgtta attgatagag
     3181 ctactgccta ttctaccatc ttagtaaatc ttgcttccga tggtgaactg gttcaattca
     3241 agatttattc ttatgcgaat gatgctgttt atcctgtgca ggagaccctt actatgcaaa
     3301 caggaagtgt atatggttct gctgaaaatc ctgttgtttt aaatggcact ttcaatatag
     3361 ttcttacacc tccccaggtt aaccttgtaa gccatcaaaa tacctatcgt cttacttgga
     3421 atgcagttcc caatgccaat aactatcgta tttattcctg cagtgaaccc tacggaactt
     3481 atcagctggt gcacacaacg gcaaatttat attgggaaat tcccactact cagcaaaaat
     3541 gtttcttcaa ggttgtagcg gagcaaattg gcatcagtaa aggcaaataa atgagaaaaa
     3601 ccatccttgt tgctctgctt ctgcttttgg cattttccct ttttgccaaa gagtggaatc
     3661 agtattactt ccgttttgaa ttggcagata agacaactct gccggaaata agtaacatca
     3721 tttctattga taatgttcgc ggtaattggg tttatgctta tgccaatgat gaagaataca
     3781 gtaaattcag ggagttgggc ttaaaaaccc aactcctgcc ctctcctgca tctctaattg
     3841 aaccggtaat gagttccgat gtgaaacaga caaagttatg ggatagttat cctacttatg
     3901 atagttatgt aaacactatg aattcctttg ccgctaatta tcccaattta tgccaaattg
     3961 taaatgccgg aactacggta aacggacgca gtttgctgtt tgccaaaatt tctgataatg
     4021 ttaatatagc ggaagcagaa ccggaagtta tgtatacctc aacaattcat ggagatgaga
     4081 ccaccggctt tgttttaatg ctacggttaa tagataccct tttaagcagt tatggaaccg
     4141 atcagcgttt aaccaacctg gtaaataatc tggaaatttg gatttgtccc aataccaatc
     4201 ccgatggaac ttattatgga ggcaataact ctgtttccgg agcccggaga tataactaca
     4261 acggatatga tttaaaccgc aattttcccg atccaaacgg caatcaatat agtggacaac
     4321 ccttacagca ggaaacaacc ctgatgatga atctggcaaa taatcatcac tttgtttaca
     4381 gcgttaattt tcatggtggg gcagaggttg taaattaccc ctgggattat acttatacag
     4441 ctcatcccga tgagaactgg tatatttcta cttcttttgt ttatgctaat aatgctattg
     4501 ccaatggacc ttccggttat tttaccagtg tttcttccaa cggtataacc aatggagctg
     4561 actggtatgt tattacgggt ggcagacagg actggatgaa ttattctgct cactgccggg
     4621 aagttactat agaaatatcc aacactaaaa tgccttctgc gtcaactttg cctgggtatt
     4681 ggaattataa ctatgaagca atgatttcct atctggaaca ggcaatgtat ggaattcacg
     4741 gaattgtgca agacccttac ggaaatcctt tatccgctac gattactgta aacggatatg
     4801 ataacagcta ttccacagtg attaccgatc ccgcaaaggg tgatttttac cggtatttaa
     4861 gccctggaac ctataatttg actatttctg ccagtggctt tcccgataag accattagtg
     4921 gcgtggttgt taatgccaat actgccactt ccatttccgt aactatgggt gaacttcctc
     4981 actatcaaca aattactctt agtccgggat ggaatttgct cagcttcaat gtggatttgg
     5041 gaacgaataa tttcagctct gtttttggca gtaaccttct gcagattaag gatacagcaa
     5101 aaagttatgc accctcaatg ccttcttatt ttaatacact ttcatcgcta caaagtgcca
     5161 agggttattg ggtgaataat tcttctgctc aaaatctcag tattcaaggt cagttactga
     5221 atacttcaaa ctatcctatt gccttaaata gcggctggaa tttaatccct tatttgccgg
     5281 ataattcctt gcctgttgcc agtgccatag cttctatttt aactaaaaca caggaagtgc
     5341 gttacttatc ctctgtttgg aatcctgttt cagggggagc tttaagtgtt ttagaaccgg
     5401 ggaaagcata ttggataagg gtctctgagc cctgtcaatt gctgtatccg taagcggaag
     5461 aacgatgtcc tcagcgttct ctggcaacct tgtctttttg taaccacgct tatttcggca
     5521 gcgaaataaa gcatgagagg tggttgaccg ggaatttaac cttcgggaaa agtcaggagt
     5581 catttgcgcc ttaataactt tgttctttac gatatctttt aaggcgcaat atgactcgtg
     5641 acgattttat atcagcaaca agtagttaac ggattttttt attttttctt tcacagattt
     5701 cccggataac atagataaga ttttataatt tcaggttaaa acctcaattt tattcctata
     5761 tttcactttt ccaccttttc gctaatgtcg tcaccaattc tcactgctaa taaaaggaat
     5821 aaacagaaaa ggacacggat gttcttttcc cagaatgcat tgcttaagca ttgctcaccc
     5881 attactaatc ccatccgtaa tgggtaagca atgggtaagc aatgctatta aggaagagat
     5941 aactaaacta ttccggtgat gacagatacg caagtgtata gagttcccag ttgattggag
     6001 cttaattttt gcaggtctgg caaggtgaga atattttttg cacccggcat aagcgcctgt
     6061 aaaaggggat tttcactctg tttcaggatt cgggttatta aatattcttg ttctgcaaat
     6121 ttgggctgag aattgatttt tcctttcggt tttctatatt gaatgcggaa gttgcgtgtt
     6181 ttattaacca aatagcggta gaagggattt ctcattttag cgggtgacca ttgatgatga
     6241 gcgtatctca attttgccag ggcaggtgaa agagcagata agaagctgta atataaaagg
     6301 ttatccttct tttgctccat tggaatctgc attgccagac ggtaaaattg atagtccaaa
     6361 aagggggttt cggaacggaa ataatagcga ttgcggtctt ccccttcaaa tgcccaacga
     6421 gcagaacgtt ccgctaaaag agcaaaagca tatttatagt tataatcctt tgtcggataa
     6481 gtagatatat gattaaggag atattcttcc atcgccttct gttgaatgcc aaatagttcg
     6541 gctgatgcct tgggagaaaa aacagcattc tgattatacc agtaattcag ccactgtttt
     6601 tcgtcagtta attgcttatc aggcaaaaga taacgcataa ctttgtcacc gccatctccg
     6661 gttaaaaaca aagcggattc gggatataga gctaaaatca tttccaaata ttgcagaaac
     6721 atagccacac agaaatagtt caaacccgct ttcaaggaaa ataacttctc ataatgttca
     6781 ggagcacaag gagatagctg taaaaccctg tgcgtctttt tcagtaattc tgctgtctga
     6841 acagcgatta aaacatcatc ttgcgcatcc tgattggcat ccagaaagga aattgctgta
     6901 aaatctatat ttttgctttg aagcgctcct gccacacaac gcgaatccat tcccccgctt
     6961 aaagatacaa cttttggaag ctctgtatga taggaaacac agatagctgt aaatgtttct
     7021 acaagttctg ccagccattt tttatgcgac ctgccaaaga ggggctctat aaacctcaat
     7081 tcctgctggg aacttatttg caattcgtca gttagccaat catatacacc taaagttcca
     7141 cccgccaaag tgtctatttc ccggtaaaaa gttccgcgtc ccggaacata agttagggaa
     7201 agatacaaag ccagaaacaa aggatccaag gttagttgat tgctgattgc tttaacaaaa
     7261 gatatatccc tacccaatag gaaatattcc ttttgttttg tatagtaaac aggtaaacgg
     7321 gctaaagcat cattggcaag aattattttg tgtaattcag gatttacggc aaacaggaaa
     7381 aaactgttgt taatttcttt gagctcggaa ttccacctat tttgcatatt ggataacggg
     7441 ttttgagaaa tttccgcctg ctctaaaatg tcttgaaaag cttgggttaa aaaggaatta
     7501 tcctgtagct cgtgaaatat ttgccaaccc ctgtattttt ctgtataaac ccgatagccc
     7561 cgatgttgag aaattgatat cagcacttcc ctgcaaggca taagcttggt aaattggaga
     7621 tgcggaaaag tggcttccaa cctttgcaga taattgtcta tggtattttg ggtttgctga
     7681 gttataccct gagcgttaat caggcaattt attccgggca ttagttctcc ttatgccatt
     7741 tcttaaataa aaaaccattt gtcttctcgt gtaagcagga atcctttttg ttactcctgc
     7801 gcacgcaaaa atccctctaa atctactaaa ccctctttgc ctaaagtgat tatctgtcaa
     7861 atgctttttc gtttttccaa gttttgaaaa aaatccttgt caagatttgt gagacgacta
     7921 tgatagcaat ataaaagaaa aaaggatttt aggaaaatgc agtttgatgt ttcctatttc
     7981 tgctttgacg gcaacagcgg aaagaaaaat caggacagag ttctggtaaa taaagacctt
     8041 ttgacagagg gaattaatca tctgcagaat cttgataacc tcttttgctt tgtagcagat
     8101 ggaattggct cgttggcaaa cagtgaaaat gcagctcaat ttgttttgga acagctgaaa
     8161 tatctgcctt taaaagccga acaaaactgg caggaaataa tagataactt cctgcaggat
     8221 ataaattatc agctggtatt gtggaatcgg caacaaggtg atttttttga ttccgctaca
     8281 actctttgcg gtttaatcta tcaggaaggc aaatttctga ccgtaaatgc aggcgattcc
     8341 gaaatttggc tttacaggga aaaatatctg caacggctga ataaactgaa tgtgctgtgg
     8401 gaagatattc ctaatagccc tattaccaat tattttggca ccgattatcc gaacctaaag
     8461 cttgatttta gctcagcagt ttcttctcta aatccgggag ataaaatagt agtaactacc
     8521 gacggactcc ttaaagctat tagcaaaaaa gaactaattc aaatcctgga agaagatagc
     8581 cctttgtata taaaaattga ttccttatac cataaactct gcgctaaata taatcccgac
     8641 aatcttggag ctatctttat ctccgaaaaa agcaacggtt aaaataaggt tgagagggtt
     8701 gagaaggttt agaaggttta gagggtttat atataaggtt ttgaaggttt ataaagttta
     8761 gaaggttgag ctatcattgt gtcgctctcc gagcttttgg aaccagggaa aaagaaaaaa
     8821 agggcttaca ttttacgagg ggcttacgcc accatcgcta tcattgtgtc gctctccgag
     8881 cttttaagaa atctaaaccc tctcaacctt ctaaaccctc tcaacctcta agcctgtatc
     8941 tatttaatta ccgccaactt cgtttttgcc aggcaatttc ctttagtatc ctgtaacacg
     9001 agaaaataaa ttccggcaga tactttaaaa ccgttattat ttttcaaatc ccaggataag
     9061 gtttgttctt tcaatgtttc agcaaggtcc aaattcctta ctttctgacc tttgaggtta
     9121 tagatagtta atttgcaggg ttcaggacta actttactta aataaatatc agcataagat
     9181 agtgctggat taggataggt agatattttt gcctgtggta tttgaggaat aacggggtca
     9241 tcaattccta ccagaaaacc ggtcacaaag aaacaacccc cagagttcaa aacataaaaa
     9301 gaaggggtat tgatataggg aaagatatct atctgtttaa ccggtgcatt aagtagagga
     9361 tgattaaaaa aggttacttc ccctgaagaa ttcagcatac cgacaaagca actatctgcg
     9421 taactttctc tccaaccaag ggttacatta ttattataaa ttggtcctaa gcaagaaaga
     9481 gctgaagtat aaaggatatt tgtccaattc gcgccaaaat cattagaacg ccaaagtccg
     9541 tctgaatcac ttcctacatc cataatagca tataaaatgc cattatcgtc aaacctgaaa
     9601 ctacttaaat tgctaatgga tgattgctgc cagttaagtc cgttatcatt ggaataataa
     9661 accatctgac cattattagt tatgatattg ctatcatacc aggcaataga attgcattta
     9721 tttgagccca gggatgttat cctggtccag ttatgtccat tcgctgaata aaacaaacca
     9781 tcccgttctc ccacataata taattggtta tggggattat actttacaaa attaggcaat
     9841 ataaaccaat cattcagttg ccagttatgc gtgttaatat caaagttata aataccatca
     9901 ctgtaagaat aaattcccat agcaaccagc atcgtgtccg catttagcat ataagtacct
     9961 ttaatagtta aacccgtagt aaaataggaa taactattcc agataccatt ttcccagagg
    10021 caaagttttt tctgattagc tatctgaatt ccaaaacttg ccggcacctg gtctaaattg
    10081 gcacaagaaa aatcgtctgc agtaggtcca aaggtatcaa cttgaaaggc aaaacagaat
    10141 actgcggaaa ttagcagtgt aaaaataaaa atcttcttca tctttctcct cctctttgct
    10201 taaagagagt aaataaaata ttaaaccaac ctcgttgtaa gctctataaa aaaaataagt
    10261 gaaaaataag aataagcaac ttagataaca tagagcaaca acacggattt tacggattaa
    10321 aaagtatctt ggatgtaata ttacttattt catcaatcct gctaatccaa gtagtacagt
    10381 tatttgagtt ctatttttta tttactaccc cttgcttaat gcattcttca tttcttgtgt
    10441 taattttctt gcttcttccc ttgcagcagt ggcaaagtcc tttcctttgg aggcaaaaat
    10501 aattcccctt gagctattga ccagaatatt gggtgtttca gaactatctt ttgcctcttg
    10561 taaaacggtt ttcaggtctc caccctgagc tccgattccg ggaataagca atatgcgatc
    10621 ctttaataag ttacgcattg ttttaatatt tgcgctatgc gttgcgccaa ctacagctcc
    10681 gaaattctct ttgggataat cctgtaacca gttagctatt ttttctgcca taccttcttg
    10741 caaaaaatcg gaggcagaag gattggaagt taaacacaac acaaaagcaa aagcatttct
    10801 gtcctgaacc gggtgcataa catcgcttcc cataagagga ttaagggtta tggcatcaac
    10861 tttgaggtct gtaaaaaaac tttcaaggta ggcattcata gtattgccaa tatcgccaat
    10921 tttgcaatct aaaattacgg gaagattatc cggaataaat tctactgtct tataaagggc
    10981 atccaaaccg cgaattccat ctgccaaata aaatgccaga ttgggtttat aggcacaagc
    11041 ataatccaaa gttgcttcaa taatggcttt attaaattcc caaataggat tctcgctatt
    11101 tttaacacct tccggaagct gagaaaaatc ggaatccagc cctacacaaa gaaccgattt
    11161 tattgcctga tagcgttcct gatatttata ccaaaacgat tttgacgcta tcttcgccat
    11221 ctgtttcttc cagtgagacc ttaattattt cctctttgga agtggggaca tttttgccta
    11281 cataatccgc tttaataggc agttcccgat gaccccgatc aattaaaacc gccagttgaa
    11341 tttgtttcgg tctgccaaaa tccattaaag catcaatagc agctcgaacc gttcttcccg
    11401 tataaagaac atcatcaaca agaactacaa ccgcatcctc aatatcaaaa tcaatagcgg
    11461 aaccctttat aaccggctga tgactaatgg tagaaagatc atcacgatat aaagttatat
    11521 ccaaaatgcc aaccggaact tcctgattgg atataagttt taaataactg gaaagacgtt
    11581 ctgctaaagg aactccccgg gaacgaatgc ccaccagacg gattttttcc aaaccgcggt
    11641 tttgttcaat aatttcatga gccatcctct gcagagaacg ctccatttgg tctttgtcca
    11701 ttatttggct tttaacgagc attttacctc cttaaagagt ggaaaggtga aacaaggggt
    11761 gtaaatagta aaggaaaatt catctgatat ataggggttg agggtttcac ttttgtctcc
    11821 ttttactcca atgcagattc tactttggaa atgtaattat attttcaaaa ctaacttgga
    11881 ttcctgcttt cgcaggaatg acaagaaaaa cttcattaaa aaaatcatat agaatgcgga
    11941 ggaaaagcga cctccggtta cccaaaacag gtttgcaact caaattatct cctggtttca
    12001 cttttcactg ttcacctttc actgttcact tttcaccatt cacctttcac ttgataactg
    12061 ctaacacatc ccctttggca acattagcac ctccggtaaa aggtgcttca acaagcactc
    12121 catctactgg agaaggaata ttattataca ttttcattgc ttccagaaca aggacagttt
    12181 ctccttgctt aacagggtca ccaggttttt tttcgtatct gataaacatt cccggcatcg
    12241 gagcggtaat ttttgtaccg ccttcggaat taactacagg agcaggttta ggttccggag
    12301 ccggattaga aggttttact tgaggattgg gattattagc cggttttaca attgtttcgg
    12361 aaataacagg tgcaggacgc gaattaacta ttttaggggt tcctcctttt tccgaaacct
    12421 ctaccagata gtaggtatta tccacaaaaa catcaaactg acgcaggtct tggggttttt
    12481 cctgcggttc cggtttttct atcaattttc ctgctttagc tttagcaata agttcttgtt
    12541 cttttttaac atctgccagg gttttaggtt tcatttcttc cggcattggt tccaagccat
    12601 atttaattct caagaacctt tttccggtaa tgttatagag ggcaacgatt aattcatcat
    12661 caatgttttt agctaagccc tcacattctt tatggacttg ttccattgca ggaggaataa
    12721 catctccggg gcgacaggtt attggtgttt ctcctctggg ataacctttt aatgcctttt
    12781 tctgcacttc cggattgata ggaagagtag tttttccata aagaccatag caaaggtctt
    12841 tcacctgttc cgtaatccgg gaatattcac cttcataaga atcaaacaag gcattattaa
    12901 ctgcctgaat acctacaatc tgactggtgg gagtaactaa aggaatttga cccaggtctt
    12961 ttctcacttt ggggatttct ttaaacacat cttccagctt atccaaagca tccatttggc
    13021 gtaattgatt taccagattg gaaagcattc cgcccggggt ctgatgaata attacatccg
    13081 tatcaattat agagaatttt gtagtatcgg caaattccag atacttggga atgtctttct
    13141 ccatttcctt gccgattttg tttaggagct taatgtcaaa accggtatcg cgattggttc
    13201 ccaaaaggga aattaccagt ggttcaattg ccggatggga agttctgtaa gcataaggag
    13261 ctacacaagt atctataata tcaacgcctg cttcaattgc cttaaataaa gccaaatcac
    13321 ccattccaga agtaaaatgg gtatgcaaat gaactggcac ttttacattt tccttcagtg
    13381 ccttaataag ttcgtaagca tcgtaagggg caattaagcc agccatatct ttaatgcaaa
    13441 ttgtatcggc acccatttct tccagctgtt tggctttagc aacataatac tcaatattat
    13501 aaatatcacc acccattcgt tgttcagtta aagaaaaaca aatagcaccc tcaaagagct
    13561 tgttgttttt cttgatgatt ttaacggcgg ttataaaatt gcggaaatcg tttagggcat
    13621 caaacacccg aaaaatatca ataccgttat cacaacagcg ttgcacaaat gcttccacca
    13681 catcatccgc ataattttga tagccaacca gattttgtcc tcgtaacaac attgagaggg
    13741 gtgttttagt gagatgtttt ttcagcaccc gaattctttc ccaggggtct tctcccaagt
    13801 agcgatgcat agtgtcaaaa gttgctccac cccaaatttc catagagtaa aagccaactt
    13861 gatccatcag ttctgccaca ttaagcatat cttcggttct gccacgtgtg gcaaaaagtg
    13921 actgatgtcc atcccgaaaa gttaaatcct gaactttaac aggattttcc gctttgggac
    13981 ggtctggttc ataatgcata gtggtcattt ctacgcaact atgtttatcc atttatggtc
    14041 tccttttatc tacatctttt aattattctt ctttgcgtta aatcgcggta atgcattgtc
    14101 agttctctgc cataagcagt atagggttga gaaggagttc ctcttaccgg ttccttataa
    14161 gttatagatg tttcttcact ttgaataagg gctataacag ctccaatggc agcgatttct
    14221 ttattgtctt tcatcttttt gcgttcctaa tgtttaaacc gggatattgc catgcttttt
    14281 gggaggcaaa ctttcacttt tggtgcaaag tatttccaat gcgtcaatta agcgttttct
    14341 tgtttccgca ggcacaataa cggcatccac ataaccccgc gtagctgcta catagggatt
    14401 attaaattcc tgttcgtaaa tttgaatcat ttcctgtctt tttgcttcct gatcttcagc
    14461 ggcggcaatt tctttccgga aaataatatt ggcagcacct tgagcaccca taacagcaat
    14521 ttccgctgtg ggccaggcaa aaaccatatc tgcacctaaa tgtctgctgc tcatagctat
    14581 atagcttccg ccataatctt tgcgggtaac aactgtcaat ttgggaactg tggcttccga
    14641 ataacaccat aaaagttttg ctccgtgacg aataatgcca ttccattcct gatgagttcc
    14701 gggaagataa cctggcacat ctacaaaagt taccagggga atattaaagg catcacaaaa
    14761 gcgcacaaaa cgcgttgctt tatcggaggc atcaatatcc aaacaaccgg ctaaaattgt
    14821 aggttgactg gctacaatac caattgttct tccatttaaa cgcgcaaaac cgataattac
    14881 attttgagcg taataatagt ggggctcaaa aaagatgccg tcgtcaacta cgctatgaat
    14941 aacatcccgc atatcataac tcattttagg gtcatctgga atgatagaat ccagttcagg
    15001 acataaacgc caggggtcat catttgtctt tatgcgaggt ggttcttcca tattattgga
    15061 aggcaaatag gatagtagca cttttatttt ctgaatggca tcggcatcgt tttcacacgc
    15121 aaaatgagcg tttccgcttt tactgttatg agccattgca ccacccaaat cttcttgagt
    15181 tgtttcttca cctgttacag cacgaattac atcaggacct gttataaaca taaagctggt
    15241 gttttttacc ataaaaacga aatcggtcat tgcaggagaa taaacagctc cacctgcaca
    15301 gggacccata atggctgtga tttgaggtat aactccactg gcacgggaat tacggaaaaa
    15361 gatatctcca taacccttta aggcatccac gccttcctga atacgagctc cacctgaatc
    15421 ttgaatacca attaagggga caccgctttt taatgccata tccataattt tgcatatttt
    15481 agccgcatgc atttctccca aagaaccacc acgggaagta aaatcctgac tgaaagcaaa
    15541 aacgggacgt ccatctacta aaccgtggcc tgtaataact ccatcggaag gaatttctat
    15601 tttttccatt ccgaaattat cacaacggtg ggaaacgaac atatccagtt cccgaaaagt
    15661 accttcatca aaaaggaggt caagccgttc acgagctgtt aatttgcccc cttgtttttg
    15721 tttggcaatg gctttttcgc cgcccatttg aagtattttt tcttcttttt ccagtaagcc
    15781 ctggatggct tcttttgtgg actgcatcag ttatcccctc tatttcttat catattttgt
    15841 atgttgaatt cggcataaag aaaaaccttt tccccattta aaggtggcaa aggtgtttat
    15901 attttggtct ttaaaacagg aattattttt ctctgccagg tagaaattcc tgcttatttt
    15961 ctctttctac agccaaggaa cttaaagcaa gcatttcttt taccttaaaa agtcaaactc
    16021 tgtttcggct ttattttgtc aaatcaaatt tccggggact aaaagtttat tatcggttga
    16081 ctgggtagag gaacaaataa ttacaataat tagcaacacg gatttaacgg cttcacacgg
    16141 atgttttttt tattaattct ctattgccta aagagagaac aaaaaatgac gaggatgtca
    16201 tttattccga aaagccaatg ctcaattctt cattcctgct tgacaaaaat ctgtctccca
    16261 aaagcaatca ctttacctcg ttggcacaaa ggataaaaga tgaaagaatt atcagcagac
    16321 ctttccaatc tggcagaggt tttttctgct ctctttccac cagaaaaaaa gtcctctgct
    16381 cagaccttct ttttaaaaca gctttcactg aaaatgcatt cctattatca gggaaaaatg
    16441 caaaccttac ctaaggcagg aatttggggt ttcaactggt ttaatgtttg gtatacaccc
    16501 ggggtttctt ctgtttcaac agccatcaga gataatatag ccaaatccta cgaattgacg
    16561 aatagaagta atttggtagc tgttgtaagt gattctaccc gcgttttagg agatggcgat
    16621 tgtggaattg ccggcggctt aggtgttatg gaaggcaagg caatgttaat gaaatactta
    16681 ggtgcttgtg atgccatttc tttatgtatt gatagtcgcg cggaaaatga caaaccgcag
    16741 gcacagaaaa ttatagattt tgtaaaaatg ctccagccct ctgtgggagc tgttaatctg
    16801 gaagatattt cccagccgaa ttgttatctt gttttagatg agctgcgtga gacctgttca
    16861 attcctgttt ggcatgatga tgctcaggga actgcctgtg taactttagc cggacttatc
    16921 aatgccttaa aattggcaga taaggaaata gataacataa aatgtgtact ttacggagca
    16981 ggtgcttcca atactactat tgcgggtttt cttttgcaga gtggcttaaa tcctgaacgg
    17041 cttattattt ttgatagcaa aggttctctt caccccggga gagaagacct gaaaagcaat
    17101 cctgataaat ttcggcagtg gtctttagct caaaacagca atcccgaatg cgttgaaggt
    17161 atagaaaacg ctctgcaaaa tgcggatgtt ttaattgcgc tttctactcc aggtccggga
    17221 acaataaaac cggaatggat aagcaaaatg gcaaaaaagt ccattgtttt cacctgtgca
    17281 aatccggttc cggaaattta tccctacaat gcccaaaaag caggcgcttt tattgttgct
    17341 acagggagag gggattttcc caatcaaata aataattcct gtggttttcc cggtatcctg
    17401 aaaggtgttt taaccgtgca ggcaagcaaa ataacggata caatggctat tgccgcagct
    17461 cattctttag ctgaatatgc ggaaaaaaga ggcatccatc cggaaaatat tgttccgcaa
    17521 atggacgagg aagatgtttt tgcttatgaa gctgcagctg tagccaggca ggcaattacg
    17581 gaaggattgg caggacttaa atttacttct gaagaggttt ttaagcaggt gcaaaaagaa
    17641 ataaatgcaa ctcgcgctct ttgtcaggaa atgatggaaa atggctatat tgcacctcct
    17701 ccaagaaatc tgattgcaga aacattacag caaacaattg tccggttccc aaattgaact
    17761 atggatttaa gcggtataaa taattttaga aagagccgaa caggcaaaat tattttatat
    17821 tttcttaagc tgggattaag tatctttatt ctctggatga tattccgcaa agtaaatttt
    17881 ataagtgccc taaaagatat aatttccttg ccgttaggta tagttctcct cctgcttgtt
    17941 ataagtttta ttcggcatac tttgcaattt cataactggt atttttcttt gcagataaat
    18001 aatttattta aagcgcgtaa aaaagaggtt atcagttcct acctgattgg tcagacctta
    18061 cgttttgcaa ttccgggagg tcctgccagt tttgccaaaa ttttttatgt tcccaatagc
    18121 agcaagatat cttctttttg gagttcaata gcggaaaaga gttttatgac ctggacaacc
    18181 tggttttttg ctcttctggc aggaatgcta tattaccggc aaagtccttt atgggtatgg
    18241 ataattcttc tgcttatcct tatttttctg ccttttctgg taacacttat tttcggagca
    18301 agcaaaaaat tttcttcctt gcaagcactt tattctgttc aatttcccaa aataatatcc
    18361 gttcaggtgt taagcaatct gctctgtttt gtccaatatt ggttgatttt aaattgtttt
    18421 ttgcctataa acttttggca gagttggata cgaatggctt taactcaatt ttccaatact
    18481 attcccatta ccatttccgg tttgggtttg cgagagggtt ttgccattca ttttttggca
    18541 ggagcaggtt ttactgcaga gcaagcggta tcggtatctt taactttatt tttcattcat
    18601 gatgttttac cggcgttaat cggtatattt tatctcttga aaagcaagaa agtttagaaa
    18661 actaaaagtt gatgtcttcg tcaaccactt tcctaaaagg ttgaggaggt tgagaggatt
    18721 tatctggttg aaacataata cacctataaa ataagaagtt tctacggaac ttttttcgct
    18781 gaaactaata tatttttcag gggattgcaa ctcgttatta tatacttatc aagcccaagc
    18841 gtatttttat ttatggcttt ttaaagggga aaaagtaata taccgcataa tggttcttaa
    18901 ctgtaaagct ctaaagttcg ttggaaatat tggtctctgc gttttcaaca acgggtagca
    18961 attccgtttt ttcctgtttc cggtattttt gccaatcggg atattgtgat aaaaagagac
    19021 cgataatggc taaaataagt cctatccatc caggcaggcc caatttatca cctaaaataa
    19081 tataagccag agcaactgta aaaacgggaa taagattggt aaaaatattg gattttgcca
    19141 ctcccaaatc gcgaataacc cctgtataaa gaataaaagc aattacggaa gcaaaaatag
    19201 acattgcagc aatagtagca agtccttcca atgaatgctg aacactgaaa aaatgctttc
    19261 cgtcaaccac aagaaacaag ggcaggaaat agagcatacc aaaaaaattc tgccaggcaa
    19321 ctatcgtgaa aggattgtaa cgcaatgtta aatttcgcac cgttattcca taaaaaaccc
    19381 cggaaaaaac tgcaatcagc aataaaagaa tacctttaaa agtagcggaa aaattatcac
    19441 tgcctaaact cataaccgct actccagagg tggataaaat aagccctaaa accactaata
    19501 aaggcaattt ttccctcaaa atcagccagg cgccgacagc agaaaaaata ggaatagtgg
    19561 aaattatcaa actgcccaaa gtggaagaaa caattctcat tccgtttgcc tcgccgataa
    19621 aatacataaa gggttcgcag aaagaaacca gcatcaaatg caaaaggtct ttcttgggaa
    19681 ctttttcccg ctttcccgta agccagacaa agggaaaaag caaaatagta gccagcaaca
    19741 aacgcagaaa agttacctca taaggtaaat atgttttata agcaattttg aaccaaacaa
    19801 aggttgcaga ccagaaaagc attgccaaaa cggcttttag ataagtaatc cagagtttca
    19861 tcagttaaat ttcagcactt cgccgtcagg aatatatttt atttttccgc cgtcatttag
    19921 gtctctacag ggtcctgctt caatttcacc actatctgta taacctcttt tttcccaaaa
    19981 accgggaaca taataatcca taagttcaat cgctacaacg ctttttgcgg atttataacc
    20041 ccacagataa gggatgaaag cccggattgg acctccgtaa tcctcggtga gtaattctcc
    20101 gtcaaaacta tatgccaaaa gggatttttc cttattggcg atttctatgg gaatagatgt
    20161 gtcgtaaatt ttgttgatac tccaaaaacg aatatagcgg caattttctt taactgcaac
    20221 ggcttgtaaa atttcagaga ggggaattcc ctgccaaaat ccctctacag accatcttgt
    20281 tacgcttgtt aggcgccctt tcacttcttt ttgaggcaaa gccaataaac tttcccaggt
    20341 aaaaatgcct ggtttttcac agctaccgct aacttcaatt tgccaatttt ctcgctttaa
    20401 tgaaccggga tgaccctcag cccaaaaaat tggtgtattc aattttgtca gtttatgcat
    20461 ttcttttctc ctatagctat ttggagtgat taaagttata atttgcctat ttttaacttt
    20521 agcgtattgt caatgttttt ctttagcaca tttcaatttg gtaattagat agtggataag
    20581 gcaaggttga ataagggatt agaattacac aatgcataaa atattaataa gttagcccga
    20641 tattttgtcc acaagggtgt aaaatgcata attgcttgtt atttagttga ttaagtaacg
    20701 actccgtaac acttccgtaa cactgccgta gttccgttac ggagttgtta cttgattgtt
    20761 acaatattat aagggggtct tgcaagtata aatttaattt tatatctggg cactaaaaga
    20821 ccatcgctat ggttgtgtcg ccctgacggg ctttaatata tctaaaccct ctaaaccttc
    20881 tcaaccctct aacccttatt tttcaagggg gttacgtacc catcgctata attgtgtcgc
    20941 cctccgggct tttatatggt ataaacctta aaaattccaa ttttagccta atttccttgt
    21001 taaattcatt cttcattttt catttatcat ttcctcaact ctctcaattc ttttttgcga
    21061 gtggttaata aggacatcaa tcttccggta ttgttacctt gccatcctgc tttgttctca
    21121 ttttcctctt gactattctc tttgatatca taaactggag taaaaaaatt tccagggagg
    21181 aaaaaatggc aatatattct gtaaacgaag taattgaaat ggcagtccaa attgaaagaa
    21241 gcggttatgc tttctataat gaggcagtta aaagaaaaga ccttgatgaa aaagcaaagg
    21301 actttttaat ttatttacgg gatgaagaat tgagacatga aaaaaccttt ttggctttga
    21361 gaaatgaggt agatcttcag gttttacagc tctccgttga ctgggaactt attgccgaat
    21421 acctgaaaac aattgtagag agcagaattt ttagcaatga attctctgcc attcaacttg
    21481 ccactaaagc caaagacata aaagaaattg tggaaaatgc gattactttt gaaaaggaca
    21541 ccctgctcta tttccacact ctttccgata atattaacga atccagaact cataatgctt
    21601 tacggaaaat aatcaatgag gaagtaagtc atattttgaa actgagcgag atgaagaaaa
    21661 aactggtgta actatcccgt attcttgcat aaatagatta tagaaaaaat ccagaataat
    21721 taaaagaaaa ttacagaaat tgaggagcaa tcaagaagga taaacaaaaa gcaagacgaa
    21781 caaaagaaag ataataaaaa acaagcaata atggctaaag gtaaacacag aaaaaataaa
    21841 aaaagcaata ccggaaggat catcagcatc cttctggtat tgtttatttt gcttattttg
    21901 gtctataatc ctatttctct gaggttaaca actattggag ttgcaattta ttatggactt
    21961 aatccggttg tattttaccg cgtgattaaa acggaaagtt cttttcgcag ttttgccatt
    22021 tctgatgctc aagctattgg tttgggacaa attaaggaat ccactgcctt ctatatacac
    22081 aaagatcata aaaggggtta tctcttttta ccggtttata atttaaagct ggcagcaaaa
    22141 tatttggtct atctgaaaaa cagatataat ggcaattgga gcttaacttt agctgcttat
    22201 aactggggtg aaagtaatgt taaccgccgt cttaagggca ttgaaattga aaaagataag
    22261 gactatagtt ctcttttcag agatattccc gagacctatt cttatatcac gaaaattctt
    22321 tatcccaaaa ataaagcttg acgagaataa cacatcagaa aaataataat ttttagaatt
    22381 catttaggag tgatttatgc tgcgtaacca aaatggaatc tcggtatata ctgttctcag
    22441 tatcattctc ttcattgtct tggtatttat cctggcattg ccgaatttct ttaatctgga
    22501 taaagaaaag aaccttgagg actgtattaa taatatgaaa caaatttggg tggcaacaac
    22561 tgattatatg cgtgatacca atgcagattt taatggtgat ctttcattgc tgataaaaac
    22621 acctaaaaaa gatgacccca agaatactta tttgtcttcc aatttgtatt gtcctgaaac
    22681 cagtcatcag aaaaaagaat atcttgttta cggaaaatat gtagcagaac aaatcggaac
    22741 agaaataaag cataactacg ggattattat tctctgcccc aatctggctc aataccccaa
    22801 gcatattatt gaaaagggat tctatgaaaa tatggagccc acacaactgc aaaattatat
    22861 gtctgaagat attgattata ttaattccga aactggctta aatggagcta aaaaagttga
    22921 attgataaac aaatatatag aaatctggaa aaccgatcct gatgcctttg ccaaacgcaa
    22981 agccaataca actgccctca aagcaattct ttttcccgaa aaattcggga taaccaaata
    23041 acgaacatta ttctaattta ggttaatatg agattcaaac agcatcttga agttatgatg
    23101 ctgtttgaat ttttttatag ctaacaactt ttaattcagg taaaagaacc cgatgtttta
    23161 caatgaattt cttgagcata tcttcaaacg ctattccgga aatgtgaaac tggaattgaa
    23221 tagaatgcag ggtttgctaa atgatatggg taatccagaa cgcaaattaa acggttttca
    23281 tattggggga actaatggaa aaggaagtgt ttgtgcaacc ctggaagctc tttgtaggga
    23341 aagtggatta aacactgcct taaatacttc gccgcattta ataaactata atgaacgctt
    23401 ccggattaac ggtaaagaac cttcttttga aacaattctc aacaccttcc ttcgctatga
    23461 aaatctgttt gataaatggg aagcatcttt ctttgaaata actactgctc ttgctttttc
    23521 aatgtttagt gaacaaaatg tagatgtttc aattattgaa gttggcttgg gaggtcgttt
    23581 ggatgctacc aatctttttg ttcctgatgt tgccgtaatt accaatatcg gacttgatca
    23641 tataaaaacc ctgggaggaa ctttagaaat aattgccggg gaaaaagcgg ggattataaa
    23701 agaaggtatc cctttggttt taggagacat agaggattct ccccgctcta ttattgaagc
    23761 tgtggcaaaa gcaaaaaaag ttcctgtttt tcgctataaa cgcgattggc agacctctat
    23821 caagaatgat aacctaaccg gtataaattt tgactatagt tttggcaatt atgaatttcc
    23881 caatctggaa gcaaatttaa taggagaaca ccaggcaatt aatattggtt cggcacttac
    23941 cgcttttatc ctctatgcgg aaaagaaaaa attgcctgtt gcggaagaag ttattcggaa
    24001 agcactacaa catatttcct ggaaaggtag aatgcaagtg ctttcccaaa agccgattat
    24061 aatcattgat ggagctcata atgtgcatgg tgtgaaggca ttaatgtcca ctctggagaa
    24121 aattttcccc aaccgcaaag taaaatttat ggtctccatc ttaggagaca aggactataa
    24181 acaaatgctg tctcttatat gtcctaaagc ggaacaaatc tatgttgccc gcaatcaatc
    24241 cgaaagagct gcttcagttg aagaccaggt agccgttata gaagaatata atataccgta
    24301 taaaacagct cctactgtcg ctgaagccta ttctttggct gtaaaagaat gtaaggagga
    24361 tgacatttta attgtttgtg gctccctttt tacagttggc gaagtgcttg aaatcccaaa
    24421 agataatgca taaagaactg gaaaattacc tttctcatct gttgcctgaa ttacaaaaaa
    24481 cgggaatctc tattgatgaa cagatagaaa ttccttatgg agttcaatta aaggtctccc
    24541 gccataatta taaaagtatt ctgaacatct attattcaca aaaaaagggt atttcccctg
    24601 ttcccacagg aaataataat cccttaaaaa aactcttatg ggatttgtgc cgtttacaag
    24661 aagaattaaa attggatatg cctccaatgc atacctggaa tgcctggata ggaagcgatg
    24721 aatgcggaaa aggtgactat ttcggtcccc tcgttgtttg tgcctttgcc tataaaagag
    24781 atagcgaaaa tgaattcaaa aatgttggag tgaaggatag caaaaaaata aacagcgaag
    24841 aaataaaacg aattgccaaa tatctctata agaattatgc taactgcatt cattgcattg
    24901 tgattaaacc ggaaaaatat aacgaactga tagccaaatt caaagcccaa aagaaaaacc
    24961 tgaatgattt gcttgcctgg ctccattcca ctgctattat cgctttgcag aaaaaccatc
    25021 ctgaaagcca aggtgtgctt gttgaccaat tcagtgtgtc acataaaaca aaatatgctt
    25081 tacaggaaaa acaatttccc cttccctgca ttgaaagaac cggagctgaa atagatcctg
    25141 ccgttgctgc cgcttccata attgcccgtt atcaatttct ggaggcattt gaggcaatgc
    25201 gtaaattcta tcagctgaat tttcctcaag gcgctaatgg taatgttatc aaatatgctc
    25261 aggattttat taaacaatac ggagaaaaac gcttgggcga agttgccaaa ctgcatttca
    25321 aaaccaccaa ccagatagaa aatatataaa taatttgaca tagaaccatt attagatatt
    25381 atgtctgtta aagaagaaag aggaagaaaa aatgaaaatc aag
//
LOCUS       DAOPFL010000014        25135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_1999_length_25135_cov_101.788477, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000014 DAOPFL010000000
VERSION     DAOPFL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 25135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25135
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_25135_cov_101.788477"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1745
                     /gene="ptsP"
                     /locus_tag="PLG69_02155"
     CDS             12..1745
                     /gene="ptsP"
                     /locus_tag="PLG69_02155"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPI42344.1"
                     /translation="MQKLIGTALNSGIFIGRAIHISLPEPEIENGNIKREDIPREIDR
                     LQNSLSAVEEEINQELHSSNLPPNEAEIISTQREILRDPEIYKQLLTAIQNKLHFATQ
                     AVAECFANIINHFETLKVETFAVRAADYRDVQMRLLNALSGGKNALIHKLGKDQIPVL
                     QEIVPSLVSKLAREGIKAYLVAKGSYNSHSSILSRALGLIAVANIPCLLEAVKDDDLL
                     IVDGYSGLLFINPDEETLDFYKQKTKQEQRKQEQLRKLQSSASKTATGIKIKLLTNIG
                     VLDELEAIKNLSCEGIGLFRTEFLYLGKQSLPSEEEQFAVYKELAERMNPLSITIRTF
                     DLGGDKLAPLSSEKQEENPYLGNRGIRFSLSYPDIFSTQVRAILRASAFGNVQIMFPM
                     IIDAEDFRKAKRFVQNCQQELSAQGIACKLKIPLGAMIEIPSAALCSEELARECDFLS
                     IGTNDLVQYALAADRNNERVSSYYIQHHPAVLGLIKQTVKSAEKYQTPLSVCGEMASN
                     PQYIPLLIGMGITELSVNPGSFLVAKSIIRKCDAHLFALTEDLNFATPLSAVEHLIEY
                     DLKPYYQNREE"
     gene            1746..3098
                     /locus_tag="PLG69_02160"
     CDS             1746..3098
                     /locus_tag="PLG69_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPI42345.1"
                     /translation="MLKYEYHNLLASKHIRSNLPKEKVLDCIDQVKNAHLEIMAEHNA
                     NLLGFYDLPDCSTKHIDDFIANLNPVFDTMLVLGIGGSALGNKALYSALKTEAELPRK
                     LFVYDNVDPVFLYEILSQINLEKTLVNVITKSGTTAETMAGYMIVADIIKRKFPKDYQ
                     KRIIITTDKEKGFLRQVINQEGYPGFTVPDNVGGRFSVLSDVGLLSSAFTGIDIKALL
                     QGAKDMREICWNPNIWENPAYLNGLIHFLYYRQGKNISVMMPYSNSLYDFADWYRQLW
                     AESLGKRKDIKGREIYVGQTPVKALGTTDQHSQVQLYTEGPNDKVFTFLTVEHFKHDY
                     TIPNLHPDREEVNYLGGKKLSELLNAERLATEIALSKAMRPNGNIVFPQIDAYHLGQF
                     IMLYEIQTVFTGKLLCINPLDQPGVEAGKIATYALMNKKGYDQERNEIEQYKKDRGLT
                     "
     gene            3685..4995
                     /locus_tag="PLG69_02165"
     CDS             3685..4995
                     /locus_tag="PLG69_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HPI42346.1"
                     /translation="MDRKAKQSAPQSFPEIEESALIFNAEQGKVYHFTKRSIEVIRLA
                     NPYPAAYRKSFETNNWQCYIPSKPFPFLFNKPHINNLSSTIYPIRKNAQNKESVYKKM
                     VAYAGEDKIALLNLFSDRFWFIYCLLMREGNYAVELMNTNPALGYLLSAPAVFHPLKS
                     KKYWQAVNKLVKMKRKDILGYFGFLACETMVKTFAKIKPELCSVDLLVLLRTALQNYP
                     SILRQLSFWEVLNSISLYIYCKGLGEILNHNLIADISTRENEEKKEIIRWIEDICRIL
                     NILKAYKMNVPKHELLTMNDLNAIHDKLLEEMLQIRDVIKNHFPEPPLPDIKCNTLWI
                     EHIQNSAELYIEGSEMHHCIYSYTSNIQQGRCYVAKMLYPERLTIMYKETPKEGLKLI
                     ETHGKCNSKPASESIDLIELWLKGYFISSQEPEQPVALTSIEKL"
     gene            5358..6491
                     /locus_tag="PLG69_02170"
     CDS             5358..6491
                     /locus_tag="PLG69_02170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42347.1"
                     /translation="MKICIVSNSHPTNDVRLYYKLAMSLAKQNEVYLITTNGIVNNTH
                     NPYQIVVDASSRRLAIWLLGKKVKELKPEIIICVEPLTLLIAWVLKKKLKFKVVFDVH
                     EFFALSFSERFPRVLRFPAYLFYQLFLKQLMKIADAVFTVNQEICNQLLGRNKRKPYL
                     VLPNYPVKNVWDYECNIPGSLEQLCQMKFDFIYIGGLTEDRGIFKILKVVSLLKHDFP
                     FLKVLILGKFFKTETEKRFNQSINDYNLNAIIYYQSWIPAEKIGLLLKRCRFGLWFFN
                     PKNRRLRLSTPLKVLEYLSAGLPVITIKTPLMKALIEKNGVGICSAYQSNALADACAK
                     MLKLSDDEYNAMSKKCLELSENKYNWETMEPELFKVINGLSKK"
     gene            6488..7780
                     /locus_tag="PLG69_02175"
     CDS             6488..7780
                     /locus_tag="PLG69_02175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42348.1"
                     /translation="MKILFISYFYPPLAGPASLRNLKTVKYLSKAGNTIDVLTVGNIV
                     YNYYDNGLLAESLQNRIIHIPSFDPMSILEKLSGKNKTVSNQIYHNTPERIKLFIRQL
                     YPLDDKVLWLPNLYKAGKKALKDEKYDLLYVSCGPFSSAVAVRTLSKHFKVPYILEMR
                     DYWTLLSDYNLQGTYLNRLFSHYTEKKLLQDASLIVTVSKGIAEDISKHFGSFLADKT
                     FILYNGHDEEDFLSLPAVSQDKSKYTLSYFGALYAKRSLKWLLKALKDLQKENKLPPN
                     FSLHLYGNYHIETLQEIKQSGIEEMVKIIPQLNHQEAISAMLSSDALLLLINSDSPKG
                     TLTSKVFEYLRTGKPILALVPQNGEAGKLLQESGQNYICPMESVSAIKICAEKLFADR
                     EKELKFHYPEDKYERRKQVEELNKKLLSVAGREGKSFF"
     gene            8029..11469
                     /locus_tag="PLG69_02180"
     CDS             8029..11469
                     /locus_tag="PLG69_02180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPI42349.1"
                     /translation="MSFVHLHNHTQYSMLDGACRVDRMIKLAKEYAMPAVAITDHGNL
                     FGAIDFYKTAKNAGIKPILGIEAYIINGDLESETGKNEIRYHLILLAQNEQGYKNLMK
                     LSSYSYIKGFYYKPRISKTLLKQYSEGLICLSACVKGEIPALIYNDRMQQAEEVALWY
                     KDLFQESFYIEIQDHNLEPEKKIMPRLIDLAKKLEIPMVLTNDCHYLNKNDYEAHDIL
                     LCIQTGKLLHDPDRLHYGTNQLYFKSPEEMKELFPDVPEAYNNTLKIADMVDLELHYD
                     KFLLPNIETPPEFKTMGEYLRFLCYENAKIKYPDLNETIKQRIEYELEVIDRMGFNGY
                     FLIVKDIIDNARKQSVPVGPGRGSGAGSIVAYLLDITQIDPLQYGLVFERFLNPERIS
                     MPDIDIDFCAQNRSKVIDYIIQRYGRESVTQIITFSTLGAKSVIKDVARVLSVPASEA
                     NAITKTIQGNKTLDEAYNQYPEFSSLINSNDLYRTIFKHSKVLEGLVRQTSVHPAGVV
                     IAPGDLTDYVPLACSTQKGEEKVILVQFEGKWLNDLKLLKMDILGLKNLTLIQKTIDL
                     VKQAQDIDIDINHISLEDKKVYNILGKGETDGVFQFESDGMRKYLMELKPNKIEDLIA
                     MVALYRPGPMQFIDSYIARKHGREKVVYDHPLMENALKETYGVTIYQEQVMQIAREMG
                     GFTGGEADSLRKAMGKKNSDLMQQYQEKFRQGAKAQNVPDAVIEKIWQDWLRFAEYAF
                     NKSHATCYALVAYQTAYLKAYYPVEFMAALLSLEDDPTKIPGKIEVCKKMGIKIIPPN
                     INRSDSEFRVHGKEVLFGLRAIKNLGDAAMRDIIEDRNQNGPYTSIFEFCNRLDSTSV
                     NKTILESLIASGAMDELEGTRAQKWNVIELALQFSSNDQKDRKRGQSTIFDFMDTEDE
                     DNNYNPPLPSVEPWSYIYQLEKEKEVLGFYLSGHPLFEYRSLIKHLTNANSSTGKTKN
                     NGELLIAGIINNISKKKDNKGNPIAFVEFEDLYGKFEVPLFNKDFMQYQKKISPGKVF
                     FIIGNKSTFNGNEDSILRILPNALIDFKELPSELSGEIKINIPNAHLKNGLLTELGNR
                     ISRNTGKFKLIIKIQSEDLNDYQLESRKLFFPDEALLNWLEKEKIDFQVRVATNGKNN
                     "
     gene            11453..12559
                     /locus_tag="PLG69_02185"
     CDS             11453..12559
                     /locus_tag="PLG69_02185"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPI42350.1"
                     /translation="MEKIISFCLLLFAFSFLFAQEIFYEHYGNEVEIWVVLPYNSINF
                     KKGSDEALYQVSLQISDAKKKQVATLGKSQVIPKCNWLNDCGIPIHFQQELPQGSYNV
                     ILQIKNKAMGERRSYSRQFIIGSSSTEIGLSWIIAKREGLEYLPHTLSGLVSDEVKLY
                     QSYSISLDSLKITIDNQVLTVKNPQNPIVLDLLPYLQTDAQNLMNLIFYENNIYYKME
                     PFLFTPWYAYSLRYSLEDQIRQIRYIANQNEWKYLRRLPKEKQAEAIETFWKANDPSP
                     GTVRNELREQFYQRVMIADERFTLHKKIAGWTTDQGRIYIKYGEPDDVYSEVYALDKY
                     PYIIWYYYDKNLEFIFADITGYGQYTLRNKDEEY"
     gene            12546..13883
                     /locus_tag="PLG69_02190"
     CDS             12546..13883
                     /locus_tag="PLG69_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPI42351.1"
                     /translation="MKNIRLFFSLVIILFAFSTLLGSDKLNMFIDYNRFLDKNKNTIL
                     LLDYQIPYENLAFLATNNGYFAELNVKVKITEADSIFLEHEINDIIGVKNKADTGNRK
                     KSYLNRLSFTLHKPLYTIQFSALDINSQKNFFYEFQIETLPPSSLLSDIELNSEVKPD
                     TSQYLVKFKRNNVLYRSEPSILFNKSINDYIYLYLEIYNWNSTSESCLLNLSLEKDSL
                     VVMDEYIDFQPGPGSESINLKIPLKDLNPGFYNGTIVLQSAERTEERNIEFAVIEEKE
                     EEFFLFANPDEEYELMRIFLGNKIPSDWGKMNQDTKRRYITQFWRDMAFSTNRSVQSI
                     MDLVQKRIDYANRNFTHFKQGWTSDMGRIYIRNGPPDDIQRDTSSDESRFVRKDYQIW
                     KYVSGKKPVYMFIDIQMNGNYRLIYVENDDLEASNPDWMRYVGSDFDTSKLDN"
     gene            complement(13880..14266)
                     /locus_tag="PLG69_02195"
     CDS             complement(13880..14266)
                     /locus_tag="PLG69_02195"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPI42352.1"
                     /translation="MKKYSLQDFSHIGEDLAARYLVSNGYTITCRNYRKKYGEIDIIV
                     EKDQHLVFCEVKTRTSHSIEWALASIGFSKQRKISRTAQLYINENPQFAKHIFRFDVL
                     LVFYYENTDTFEIKHFENAFDAILEY"
     gene            14352..15551
                     /locus_tag="PLG69_02200"
     CDS             14352..15551
                     /locus_tag="PLG69_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42353.1"
                     /translation="MKKTLLTVIALVAVLGVAQAAPFQTLGMLRTPDAYVLPNKAAEF
                     LVVGYYRDISAPKPLTEDAKDASKGFFPYTMIGVGILDRVELSMFLGDYTSLDGLVYL
                     LNAKVKIIEETLLIPQIAVGMDNILSPVEKHGAQYLDVNNDFADNPEADSYEPYSPYA
                     VASKQVVFWGIPWMFNLGIGSNRFVGQAPRARYLTGTFFSVEMSPVRDLTLQGEFDGE
                     DINIGINYAYKNFGFKLGASAIEDRFKDSDYKNNIRVALGISYLFDKYAEAKRRPDLG
                     LYASYGKKPSAGGEVVEVGETPIETGVVVPPTGTTVSPTGEIVVVPPTGTAAKPPAGG
                     EVVVGPGTQLQTPGLVTPGTAGYQQLSPEVQDLLKELQMLKEERQKAQQAMEELRKWI
                     EELKKKQ"
     gene            15705..21554
                     /locus_tag="PLG69_02205"
     CDS             15705..21554
                     /locus_tag="PLG69_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42354.1"
                     /translation="MKHNLKIIFLIVVAVSFLAVVYAQDRSQYTKEQIQEQISQEKQL
                     IRELAKMFLVDALPELVKEDPTLAEQALVKYKDVFSSMDQNEFIYLLGHFYVRVGENA
                     KAISHFTSLLNTHLNEDARKMLNLVLFQQMVHYLEQGNRKTAKDFLSAIVFENYNIDR
                     YYPTYLYIWADMAADDGEYESVINTLNSYIENRDIIMNRLLPEKMAILARVQNLNLEP
                     YYANPSPAEYKRLASQIDGIKADLTRNYNELISLKGIFYLDSIVNLHSEEMAMLDSLK
                     MVILDYIDQKVQAEETMVRGNLLFQQVKQLSSNYQKQLEIMDKILEKQYERFLTNDPS
                     VQGQNFSDMELKRLFDIEKNIQLYNNLIAELDKDIADPDLANIQEQLKRTRADYSEKR
                     TSLLIRKNDLLQTRKHISDVEEQIFNALLQEYYSLSQDKKDIDVQLAEIEQFMQEESK
                     NIFTEEQRNKIKNRIATQIAVTADSGVRNEPIRKNAQDLVANVEFVKLQLDYRNLHIK
                     EQARIAQIEKWTEQQLTERQAEILAEKRDLITRIQNFINNNPNFKAVEQPNGTFLISN
                     ADLYYNLAELQYAVYLETPTIALESYRKVVQLDPNNVNIDAALYNIGFISSQLSHQRI
                     GDNKARFYEINRTAVALDDASRYKESDFAEAISSYQRIIDNYKTSPYYDESLYRLGIL
                     YYYIATDADQPQRYYALATNCFDEIINKPDSKYKYDAIYQRGWLRLNSFQEQDFMLAL
                     NDFLTLLNAVDSGKISDPMIAKGYREDAIDNIAFCLIALDGSDFYSPAKGVAELQNIA
                     ASYKNPEIISSIIDKAAANKLNLSTIQAIDFIWLKIDINPLALENPTLVDSILQLYAS
                     AQYELREGQNFDQIAQDLYQNIITNYGKNTAWYQANMNNSNISAQLAVIKNAYEKRAI
                     RLQNEFLADPANETKLLAYYQHIDQYAAFPELQGENYVAWKAQADKTKLLLGNTLTER
                     SNLPKHYLTAIRNLQEFNAKYPQDEDFFLNEGLIYTYSFNIYNLMKDLYGEGFQPEPG
                     LPANADELFALLQSNSQRFISVLRSDAYKNPEREIQAVNILLSLADIQYDRGKLPDAK
                     ELYLSALENETLIPDRTKFTVYGKLASIAENEQNYAQAETYYRKALPYAATPDEREAI
                     INNICYQIQNSFEAAESAGNYSLAASERLRLAEELGSKDAQRVTGYKMGAQEAYIKAK
                     EYQKAIDILLELAGTRTDIGEIYYYYFRAAEIAEADTAMNNPQLAKSIRESFIAKYPS
                     SNQAFKLRMAMIQEMEKNPAQKNAAADAYLALYEEARNKTINTGNITPDALLAKAAIL
                     YSEAENKDKAVETYNRFITLYPNSENVIPFMQESADYYLAKGDTLKFEQLAKEIYQKD
                     KTKGDRYQWIATLKLSKLVYNFDNAYKNKNYTDAFKFRDEYKRLEAVYKKEGLNFETK
                     DFSSTKNYEYFAAVEKEYNDLQKRIAFLKNFDNQLTAIEKGTLMTSSPAALIKVNAST
                     TWQNHLIGGSYRRIPNFKASVKAEVAKVNKLLADADKQNIELENYRRLRALNLIARIY
                     EKGVNVINTQIGVYVRTASEVEGVRQEYKDVLDAFIATLAADYSNDLFSLAYTTHLNI
                     YNFYQLAGYTDYYTQQSYAKLQEWNLLPDYKMEEYPLGSGWTEKMDGTNVNLPVQNIT
                     SPKGVRLSCVQIPSNKELILNRTVNAKIQPEFALLHLVYPYDIKIQLNGTDLKPGVVP
                     TDTLDVNKPITTTRLAYLLPSSAWVEGQNIIELTVPNDSPEQQSLYMNLQVFIDRNKL
                     AASIPPETVMLYTDPTWRIITVNPETGVETASAATITGNFGITLDNIVGLEETFAKPI
                     WFPETAPVSSAIFEVDFYLDTEFRDGLINFVAPEVASVYLNGQELAANLHLDYDPEPF
                     RVYTSQVTIDPAKVTAGKNTLRFVVTNNSNYRGFIATVKIIKAGKGDIR"
     gene            21551..21850
                     /locus_tag="PLG69_02210"
     CDS             21551..21850
                     /locus_tag="PLG69_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42355.1"
                     /translation="MKKVFILLAVLLFFTSIIYAQAATTTTQQQPVKGFEGVRRYVEE
                     KELVIEIKALIPSTDIFQDKSDTYRKAGEYKFSFTIEDIHKQSDTLLNKWLKESK"
     gene            21847..22614
                     /locus_tag="PLG69_02215"
     CDS             21847..22614
                     /locus_tag="PLG69_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPI42356.1"
                     /translation="MKTKNIVLMIISLFMFVGLFAQPATETAAPAAPAKQGYSLIQVF
                     KDSGNWAYAILLVLVIGLVYAFVRWYQLYPKEKIDAAGFYLKLRNYIRNDQLDEATKI
                     AESYKGTTMGFIFWSALMVFKDSRKSGLTGSDLRNSVQNSLEEAVLQTVYKLDSGLFW
                     FDTLAQVATYLGLLGTIWGLLGAFAGLAGLTGAAQQTALTDGIKKAIGTTALGLMTAI
                     PLTLIKGWLLTRANKIISNIDEFSVKLINTINNAIKD"
     gene            22620..23144
                     /locus_tag="PLG69_02220"
     CDS             22620..23144
                     /locus_tag="PLG69_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPI42357.1"
                     /translation="MRQYRPSAMRQQKSQTVPQEPNLVPIMNLFLTIIPMLLMMVVIS
                     QIALLALNFSQGESGVSYGQGGGGGGAKTEKVEIHILGEQYAQENNTTLGMEIREPGF
                     KPMTISAINGYYDYVTLNKTLQEIRSRKGTLSDITVLVHPDVLYGDLMKTIDICKVNG
                     FTQVHYTATKVEYY"
     gene            23156..23701
                     /locus_tag="PLG69_02225"
     CDS             23156..23701
                     /locus_tag="PLG69_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPI42358.1"
                     /translation="MQILDKLKSSNKSERRVRKKFEEAKGLSITSLMDVLTIILVFLV
                     KNISTEVIKISAEPNITYPHTITNDKLLEKAGTTPIKIFPDRVVVGTEALEYGSPADL
                     LTNAQKRNDLLTYLKMEAEDIIQHQKAETCLLVQADYSIPCEYITEIVRAATAAGYSY
                     IYFATLEDADWLKKYSVSLAE"
     gene            23714..>25135
                     /locus_tag="PLG69_02230"
     CDS             23714..>25135
                     /locus_tag="PLG69_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42359.1"
                     /translation="MSKLLNLTLHYKGKKLDQVRYGRDFKNKFFIGSDKYLFWQILDE
                     SFPEKHLFVTKKGDQLYLQLPPGAKVSCAKDGEPVDESYLTKSFILQGNKLLLRPDMT
                     GTLAIAPNWEISYEFKEPYVTVLTPEQQQIAAQYACRPQPTADEKFSRNMILLFVILT
                     VIFVIIFDLFLKKQVSYDTTLEQKLQTIQKAERIQAEALAKPSTFEEAGEYAEVPEET
                     AGKGQPGGVPGGKGTAKSAGAIFGSGFGTFDPSATSAARPFQAVTLMEGFVTSRPGGG
                     GGGGAGPGVGGAGPGGGVGGTTFDPNATRSFTSDIGSVATKAPTVRGSEIRPEGVAIV
                     KASGDQSKLMPSGVAFGKTAVTEQTLAKFKSKQVTQVTEETISQAPAERQTIIDEVRR
                     AVEIRKGQIQSLYMKWNATIPFSGSVKIRLLINASGKVQDAIITPEGNMPNGFLTELE
                     QLCESWSFPINEEFDYEFKFRLRK"
BASE COUNT         8217 a         4524 c         4988 g         7406 t
ORIGIN      
        1 ttggagaata aatgcaaaaa cttataggaa ctgccctgaa tagcggaatt tttatcggca
       61 gggcaattca tatttctttg ccggaaccgg aaattgaaaa cggcaatatt aagcgcgaag
      121 atattcccag ggaaatagac cgtttacaga attccctttc cgccgttgaa gaagaaatta
      181 accaggaact tcattccagt aatctacccc caaacgaagc ggaaataatc tccactcagc
      241 gtgaaatttt gcgtgatccc gaaatttaca agcaattgct aactgccatt caaaacaaat
      301 tacactttgc tacacaagcg gttgccgaat gttttgccaa tattattaat cactttgaaa
      361 cgctgaaagt agaaaccttt gccgtgcggg ctgctgatta tcgcgatgtg caaatgcgtt
      421 tgctgaatgc tttatccggt ggtaaaaatg cccttattca caagctggga aaagaccaaa
      481 ttcccgtgtt acaagaaatt gtgccttccc tggtgagcaa acttgccagg gaaggaatta
      541 aggcatacct tgtagccaaa ggaagctaca attcacactc ttctatttta agccgggctt
      601 tgggtttaat tgcggttgct aatattcctt gtttattgga agcagttaaa gatgacgacc
      661 ttttaattgt agacggctat tcaggtttgc tttttatcaa tccggatgag gaaactttgg
      721 atttttacaa acaaaaaaca aagcaggaac aaagaaaaca ggaacaacta agaaaactgc
      781 aatcctccgc ttctaaaacc gcaacaggta ttaaaattaa gctgctaacc aatatcggag
      841 tgctggatga actggaagct attaaaaacc tttcctgtga gggaattggc ttattcagaa
      901 cggaattttt gtatttggga aaacaaagct tgccttcaga agaggaacaa tttgccgttt
      961 acaaagaact tgcggaaaga atgaaccctc tgtctattac tatcagaact tttgatttgg
     1021 ggggagataa acttgctcct ttgagctctg aaaaacagga agaaaatcct tatttaggca
     1081 atcggggcat aagattttcc ctttcctatc cggatatttt ttccactcag gtgagagcta
     1141 ttttaagagc ttcggctttt ggcaatgtgc aaattatgtt tccgatgatt atagatgcag
     1201 aggatttccg caaagccaaa aggtttgtcc aaaattgtca gcaggaactt tctgctcaag
     1261 gaattgcttg caagctaaaa attcccttgg gagcgatgat agaaattcct tctgccgcat
     1321 tatgctctga ggaattagcc cgagaatgcg attttttaag tatcggaact aacgaccttg
     1381 tgcaatatgc tttggctgca gaccgcaata atgaaagggt gtcatcttac tatattcaac
     1441 atcatcctgc ggttctggga ttgattaaac aaacggtaaa aagcgcggaa aaatatcaga
     1501 ccccgctttc cgtttgtgga gaaatggctt ccaatcctca atatattcct cttttaatcg
     1561 gaatgggaat tacggaatta agcgttaatc cgggaagttt tctggtagcc aaatccatta
     1621 tccggaaatg tgatgcccat ctttttgcct tgaccgaaga ccttaatttt gccactcctt
     1681 tatcagcagt ggaacattta attgaatatg atttaaaacc ctattatcaa aacagggagg
     1741 aataaatgct gaaatacgaa tatcacaacc tcttggcttc caaacatata cgcagtaatt
     1801 tgccgaaaga gaaagtgctg gattgtattg atcaggtgaa aaacgctcat ctcgaaatta
     1861 tggcagaaca caatgctaat ttacttggtt tttatgattt gccggattgc tctaccaaac
     1921 acattgacga ttttattgct aatctcaatc ccgtttttga tacaatgctg gttttaggca
     1981 ttggcggttc cgctttaggc aataaagcat tatattccgc tttgaaaact gaggcggaat
     2041 taccccgtaa actctttgtt tatgataacg tagaccccgt ttttctctat gaaatcctgt
     2101 ctcaaataaa tctggaaaaa actttggtga atgttatcac caaaagcgga accacagccg
     2161 aaacaatggc tggctatatg attgtggcag atataattaa acgcaaattc cctaaggact
     2221 accaaaaacg gattattatc actacagata aggaaaaggg atttttaagg caggtaataa
     2281 atcaggaggg ttaccccggt tttaccgttc cggataatgt aggaggcagg tttagtgttc
     2341 tttctgatgt gggtttgctt tcttccgctt ttaccggaat tgacattaag gcattgctgc
     2401 aaggagcaaa agatatgcgg gaaatttgtt ggaaccccaa tatttgggaa aatcctgctt
     2461 atctgaatgg tttgatccat tttctctatt accgccaagg gaaaaacatc agcgtaatga
     2521 tgccctatag caattctctt tatgattttg cggattggta tcgtcaactt tgggcagaaa
     2581 gtttgggtaa acgcaaggac attaaaggca gggaaattta tgtgggacaa acacctgtga
     2641 aagcattagg aacaaccgat caacattctc aggtgcaact ttatactgaa ggtcccaacg
     2701 ataaggtctt cactttctta accgtggaac actttaaaca cgattacact attcccaatt
     2761 tgcatcccga ccgggaagaa gttaattatt taggtggcaa aaaactttcc gaactcctta
     2821 atgcggaacg acttgctaca gaaattgccc tcagtaaagc aatgcgtcct aatggcaata
     2881 ttgtctttcc ccaaattgat gcctaccatt taggacaatt cattatgctc tatgaaattc
     2941 aaaccgtttt tacaggcaaa cttctttgta tcaatccgtt agaccaaccc ggagtagaag
     3001 caggaaaaat cgctacctac gccttaatga ataaaaaggg ttacgaccag gaacgcaacg
     3061 aaatagaaca atacaagaaa gaccgtggct taacctgaaa atgattcagt gctttccact
     3121 ttagtaaatg tattacagga ttctgattca gcaaatatga agtagagagt tcttcgcaga
     3181 taactttgct atctgtggca aataggattt ttacatctct cttgctactt ataattacta
     3241 tctatttctt ctgtattatt cgggataata ttctaactta ctaaaatatt atgcgattaa
     3301 cacttttctt ttagttcttc cttaatagca ttgcttaccc attgcttacc cattacggat
     3361 ggcattagta atgggtaggc aatgctaaat taatgctctt aggggagagt tgctatcttc
     3421 cacaaaatca atataatgta attttaatat tgataaatca taaaaggtgg ttgacgatga
     3481 attcaacctt ccggttttac tctttgaagc gggaaaaaat ctcaactttc taaaccttct
     3541 caactatcta aacattttca actttctcaa gcttattttt acttgacatt ttttactcat
     3601 tggggcgaga gtaatttttg aaaaagagaa gaaaagccga aaaataagca tccaaagaga
     3661 taataatgag ggaggtaacc ttctatggac aggaaagcaa aacagtccgc tccacaatcc
     3721 tttccagaaa tagaagaaag tgcgttaatt tttaacgcag aacagggcaa agtttatcac
     3781 tttaccaagc gatctataga agttatccgc ttggcaaatc cttatcctgc agcgtataga
     3841 aaaagttttg aaacgaataa ctggcaatgc tatattcctt ccaaaccctt tccttttctg
     3901 tttaataagc cacatatcaa taacttatcc agcaccattt atcccattcg gaaaaatgct
     3961 caaaataagg aatccgtcta taaaaagatg gttgcttatg cgggtgagga taaaattgct
     4021 ttactgaacc ttttttctga ccgtttttgg tttatatatt gcctgctgat gagagaagga
     4081 aattatgctg ttgaactaat gaacacaaat cctgctttgg gttatttgct tagtgcaccc
     4141 gctgtttttc atcctctgaa aagtaaaaaa tactggcaag cggtaaataa actggtgaaa
     4201 atgaaacgca aagatatcct gggctatttt ggttttcttg cttgcgaaac aatggttaag
     4261 accttcgcta aaatcaaacc cgaactttgt tccgttgatt tattagttct tctcagaacc
     4321 gctttgcaaa attatccttc cattctgcgt caactctctt tttgggaagt tctcaattct
     4381 atttccttat atatatactg caaaggattg ggggaaattt tgaatcacaa cctgattgcc
     4441 gatatctcta ccagggaaaa tgaagaaaaa aaagagatca tacgctggat tgaagacatc
     4501 tgcagaatac ttaatatctt aaaagcatat aagatgaatg tgccaaaaca cgaactctta
     4561 actatgaacg atttgaatgc tatccacgat aaattgctgg aagaaatgct acaaataaga
     4621 gatgtgatta aaaaccattt tcctgaaccc cctctacccg atattaagtg taataccttg
     4681 tggattgaac acatacaaaa ctctgccgaa ctctatattg agggctcaga aatgcatcat
     4741 tgtatctatt cctatacctc caatattcaa cagggaaggt gctatgttgc caaaatgctt
     4801 tatcctgaac gacttactat tatgtataaa gaaactccaa aagagggctt gaagttaatt
     4861 gaaactcacg gaaaatgcaa ttctaaaccc gcatctgaat ccatagacct gattgaactt
     4921 tggttgaaag gatattttat cagttctcaa gaacctgaac aaccggttgc tcttacttct
     4981 atagaaaagc tttgatttac accccaataa tgccttataa attgttaata aacatattaa
     5041 gcaattgggt tgacgaattc cgacttaagt aaatgctaaa agcattaatt tattcagaaa
     5101 cctaaagttt ctatggcata gaaagaattt gccaacccat ttttccctga ctgctatatt
     5161 tggatttaac taatgcgact atgttgtttt tctttccggt tcatagaaat tatcctcttt
     5221 tcctcttttt ctctttgtaa aacaaaactc tttttctctt tctctctttg taaaaaaatc
     5281 actcttttct tcttgacata aaagagcaga ataagaaaat ataataatta ggataattta
     5341 aaaggcagtt tccgataatg aagatatgta ttgtttccaa ctcgcatcct actaatgatg
     5401 tgcgtttata ttacaagctg gcaatgagtt tagccaagca gaatgaagta tatttaatca
     5461 ctaccaatgg catcgtgaat aatacccata atccatatca gatagtagtt gatgcttctt
     5521 ctcgtcgttt agctatctgg cttttaggga aaaaggtaaa ggaactgaaa ccggagataa
     5581 taatttgtgt ggagccctta accttgctaa ttgcctgggt tttgaagaaa aagctgaaat
     5641 tcaaagttgt ttttgatgtg catgaattct ttgcgctttc tttcagtgaa aggtttccgc
     5701 gtgtattgag atttcctgcc tatttatttt atcagttatt tttaaagcag ttaatgaaaa
     5761 ttgccgatgc agtttttact gtaaatcagg agatatgtaa tcaattatta ggcagaaata
     5821 aacgcaaacc ttatttggtt ttacctaatt atccggtgaa aaatgtttgg gattatgaat
     5881 gcaatattcc aggttctttg gaacagcttt gccagatgaa atttgatttt atttatattg
     5941 gaggacttac tgaagaccgt ggtatcttta aaattttgaa agttgtttcc ttattgaaac
     6001 acgattttcc ttttttaaag gtgcttattc tgggtaaatt ttttaagaca gagaccgaaa
     6061 agagattcaa tcaaagcatc aatgactata atttgaatgc tataatttat tatcaaagtt
     6121 ggattccggc ggaaaaaata ggacttttac tcaagcgctg tcggtttgga ctctggtttt
     6181 ttaatccaaa aaacaggcga ttacgacttt ccactcctct caaagtgctg gaatatcttt
     6241 ctgcagggct tcctgtaata acaattaaaa cacctttaat gaaagcgcta atagaaaaaa
     6301 atggtgtagg aatatgttcc gcttatcagt ccaatgcctt agctgatgcc tgcgcaaaaa
     6361 tgttaaaact tagcgatgac gaatataatg ctatgagcaa aaaatgcctg gaattatcgg
     6421 aaaacaaata caattgggaa acaatggagc cggaattatt taaagtaata aacggactga
     6481 gtaaaaagtg aaaattcttt ttatcagcta tttttaccca cctttagcag gacctgcttc
     6541 tttgcgaaac ctgaaaacgg ttaaatattt aagtaaagca ggcaatacaa ttgatgtgct
     6601 aaccgtgggc aatattgtat ataactatta tgataacgga cttttagccg aatccctgca
     6661 aaatcggatt attcatattc cttcttttga ccccatgtct atcctggaaa agctgagcgg
     6721 gaaaaataaa acggtctcca atcaaatata tcataacaca cctgaacgaa taaaactctt
     6781 tatccgtcag ctttatccat tggatgataa agtgctgtgg ttgcccaatt tatacaaagc
     6841 aggcaaaaaa gcgctaaagg atgaaaaata tgacctcctc tatgtttcct gcggaccatt
     6901 ttcttctgct gtggctgtaa gaacgctaag taaacatttt aaggtgccat acatattgga
     6961 aatgagagat tattggactc ttttaagtga ctataatctg cagggaactt atctcaaccg
     7021 acttttttca cactacacgg aaaaaaagct cttgcaggat gcttctttaa tagttaccgt
     7081 tagcaaaggt attgcagaag atataagtaa acattttggt agctttcttg cagataaaac
     7141 cttcatttta tataacggac acgatgaaga ggactttctt tccctgcctg ctgtttccca
     7201 agataaaagt aaatatacgc taagctattt tggtgctctt tatgctaaaa gatcgttaaa
     7261 atggctgctg aaggcactga aagatttaca gaaagaaaac aaactgccac ctaatttttc
     7321 tctccacctc tatggtaatt atcacattga aaccttgcag gaaattaaac aaagcggaat
     7381 tgaggaaatg gtgaaaatta ttccccagct gaaccatcag gaagcaattt ccgctatgct
     7441 ttcttccgat gccttactgc ttttaataaa ttcggatagc ccgaaaggaa ccctaaccag
     7501 caaggtcttt gaatatttac gaaccggaaa acctatcctg gctctggttc ctcaaaatgg
     7561 tgaagcagga aaacttttgc aagaaagtgg acaaaattat atctgtccaa tggaatccgt
     7621 ttctgctatt aagatttgtg cggaaaagct atttgcggat agagaaaaag agctgaagtt
     7681 tcattatcct gaagataaat acgaaagaag aaaacaggtg gaagaactaa ataaaaaact
     7741 actttctgtg gcaggaagag aaggaaagag ttttttttaa caaagaggaa aagagggaaa
     7801 gagtttagtt ttacaaagag gaaaagagaa tagttttaca aagaggaaaa gagaaaaaga
     7861 ggaaaaagaa aaataacaat gcactttgaa ctttgaactt tgaacgctcg cactttgaac
     7921 tcccgcactt tgaacgtccg cactttgaac ttcgccaact ttcagatatt ctttgacaga
     7981 tttctgtatt ccatttttat atgtttagat aaactcaggg agtgagagat gtctttcgtt
     8041 catttgcata accatacaca atacagtatg ttggatggtg cctgcagagt tgacaggatg
     8101 ataaaattgg ctaaagaata tgctatgccg gctgttgcta taacggatca cggcaatctt
     8161 tttggtgcca ttgattttta taaaacagcc aaaaatgccg gtatcaaacc tatcctcggt
     8221 attgaggcat acattatcaa tggcgacctg gaaagtgaaa ccggcaaaaa tgaaatcagg
     8281 tatcatttaa tattactcgc tcagaatgaa cagggctata aaaacctgat gaaactctcc
     8341 tcctattcct atattaaagg attctattat aagcccagaa tttccaagac cctgctaaaa
     8401 caatacagcg aaggtctaat ttgcctttct gcctgcgtta aaggtgaaat tcccgctctg
     8461 atttataatg accgaatgca gcaggcggaa gaagttgctt tgtggtataa agaccttttt
     8521 caggaaagtt tttatattga aattcaggat cataatctgg agccggaaaa gaaaattatg
     8581 ccccgtctga tagatttggc aaaaaaactg gaaattccga tggtgctaac taatgactgt
     8641 cactatttga ataaaaacga ttatgaagca cacgacatat tgctttgtat tcaaacggga
     8701 aaacttttac acgatcccga ccgtttgcat tacggaacta atcagttata ttttaaaagc
     8761 ccggaagaga tgaaagaact ctttcccgat gtccctgaag catataataa taccttaaaa
     8821 atagcagata tggtagacct ggaactgcat tatgacaaat ttctgctgcc caatattgaa
     8881 acccctcccg aatttaaaac aatgggtgaa tatctgcgtt ttctttgcta tgaaaatgcc
     8941 aaaattaaat acccggattt gaatgaaacc ataaagcaac gcattgaata tgagctggaa
     9001 gtgattgaca gaatgggttt caatggctat tttctcattg ttaaagatat tattgataat
     9061 gctcgtaaac aaagtgttcc tgtaggtccc ggtcgtggtt ccggtgccgg aagtattgtt
     9121 gcttatttac ttgatataac acaaattgat cctttgcaat acggactggt ttttgaaaga
     9181 tttctgaatc ccgagagaat tagtatgccg gatatagata ttgatttttg cgctcaaaac
     9241 aggagcaaag ttattgatta tattatacaa cgctatggaa gagaaagtgt aactcaaata
     9301 ataacttttt ctactttggg agctaaaagc gtaattaaag atgtagccag ggttttgagt
     9361 gttccagctt ccgaagcgaa tgctataacg aaaactattc aaggcaataa aactttggat
     9421 gaagcatata accaatatcc ggaattttcc tccctaatca atagtaatga tttatatcgc
     9481 actattttta aacattccaa agtgctggaa ggtctggtgc gtcaaacaag tgttcatcct
     9541 gcaggagttg ttattgctcc cggagacctt acggattatg ttcctcttgc ctgtagcacg
     9601 caaaaaggtg aagaaaaggt tatcctggtg caatttgagg gaaaatggct gaatgatttg
     9661 aagctattga aaatggacat tttgggtttg aaaaatctta cccttattca aaagacgatt
     9721 gatttggtga aacaagcgca agatattgac attgatatca atcacatttc tctggaagat
     9781 aaaaaggtct ataacatatt gggtaaaggc gaaactgacg gtgtttttca gtttgaatcg
     9841 gatggtatgc gtaaatatct tatggaactg aagcccaata agattgagga cttaattgct
     9901 atggtagctc tctaccgacc cggtcctatg cagtttatag atagttatat agctcgtaaa
     9961 cacggacgcg aaaaagtggt ttatgatcat ccgctgatgg aaaatgcgct aaaagagacc
    10021 tacggagtaa caatctatca ggaacaggtg atgcaaattg cccgtgaaat gggtggtttt
    10081 acaggtggcg aagcagatag tttaagaaaa gctatgggga aaaagaattc ggacttaatg
    10141 caacaatatc aggaaaaatt caggcaggga gctaaagccc aaaatgttcc tgatgcagtt
    10201 atagaaaaga tttggcagga ttggttacgc tttgcggaat atgcttttaa taaatcccat
    10261 gctacctgct atgctttagt tgcatatcaa acagcatatc tgaaggcata ttacccggtg
    10321 gaatttatgg ctgccttgct ttccctggag gatgatccga caaaaatacc cggtaaaatt
    10381 gaggtctgta aaaagatggg tataaaaatc attcctccga atataaaccg tagcgatagt
    10441 gaattcaggg ttcacggtaa agaagtcctt tttggcttac gagcaattaa aaatctgggt
    10501 gatgccgcaa tgcgggatat tattgaagat agaaatcaaa acggtcctta caccagtata
    10561 tttgaatttt gcaaccgttt agatagtacc agcgtaaata aaaccattct ggaaagtttg
    10621 attgcctcag gtgcaatgga tgaacttgaa ggcaccagag ctcaaaaatg gaatgtaata
    10681 gaactggcgt tgcaatttag cagtaatgac cagaaagata gaaaaagggg acaaagcact
    10741 atttttgatt ttatggatac tgaagatgag gataataact ataacccgcc actgccttcg
    10801 gttgaaccct ggtcttatat ctatcaattg gaaaaagaaa aggaggtttt gggattctat
    10861 ttaagcgggc atccgctgtt tgaatatcgc tcgttaataa aacacttgac caatgccaat
    10921 tccagcaccg gcaagacaaa aaataacggt gaattgctta ttgcgggaat tataaacaac
    10981 atttctaaaa agaaagataa caaaggtaat cctattgcct ttgtggagtt tgaagacctt
    11041 tacgggaagt ttgaagtgcc attgtttaat aaggatttta tgcagtatca aaaaaaaatc
    11101 agcccgggaa aggtcttttt tatcattggc aataaatcca cctttaacgg caacgaagat
    11161 agcatcttaa gaattttacc aaatgcttta attgacttta aggaattacc ttcggaattg
    11221 agcggagaaa tcaaaatcaa tattccaaac gcacatctga aaaacggatt actaactgag
    11281 ctgggtaata gaatatctcg taatacaggc aagtttaaat tgataatcaa aattcaaagc
    11341 gaagacctga atgattatca attggaatct cgcaaactct tctttcctga tgaagcattg
    11401 ctgaactggc tggaaaaaga gaaaattgat tttcaggtga gggtagcaac caatggaaaa
    11461 aataattagc ttttgccttc tgctgtttgc tttcagcttt ctttttgctc aggaaatttt
    11521 ctatgagcac tatggaaacg aagtagagat atgggttgtt ttgccatata attcaatcaa
    11581 cttcaagaaa gggagcgatg aagcactcta ccaggtttct ctgcagatta gtgatgctaa
    11641 aaaaaagcaa gtggcaacct tggggaaaag ccaagttatt ccgaaatgta actggcttaa
    11701 cgattgtgga attcctatcc attttcagca ggaacttcct cagggctcat ataatgtaat
    11761 tttacagata aaaaacaaag caatgggaga gagacgaagc tactctcgtc aattcataat
    11821 tggttcttcc agcacagaaa ttggactcag ctggattatt gctaaaagag aaggactgga
    11881 atatcttccc catacgcttt ccggactggt tagcgatgaa gtgaaactat atcagagtta
    11941 ttcaatttct ctggatagct tgaaaataac tatagataat caggtcttaa ctgtaaaaaa
    12001 tcctcaaaat ccgatagttt tagaccttct tccctatctg cagacagatg cccaaaacct
    12061 gatgaacttg atattttatg aaaataatat ctactataaa atggaacctt tcctgtttac
    12121 tccctggtat gcttattctc tgcgttattc tctggaggat caaataagac aaatccgcta
    12181 tattgctaat caaaacgaat ggaaatattt acgacgctta cccaaagaaa aacaggcgga
    12241 agcaattgaa acattttgga aagcaaacga tcccagcccc ggaactgtta gaaatgaatt
    12301 aagggaacaa ttttatcaaa gagtaatgat tgccgatgaa cggtttacct tgcataaaaa
    12361 aattgccggc tggacaacag atcagggtag aatatatatc aaatacggag aaccggatga
    12421 tgtctattcg gaagtttatg ctttggataa atatccttat attatttggt attactatga
    12481 taaaaacctg gagtttatct ttgcggatat tacgggttac ggacaatata ccttgaggaa
    12541 taaggatgaa gaatattagg ttatttttta gcttagtaat aattcttttc gcctttagca
    12601 ccctcttagg gtcggataaa ttaaatatgt ttattgacta taaccggttt ctggataaaa
    12661 ataaaaatac tattctttta cttgattatc agatacctta tgagaatttg gcttttctgg
    12721 ctacaaataa tggatacttt gcggaactga atgtaaaggt taaaattacg gaagcagata
    12781 gtatattttt agagcatgaa ataaacgata ttatcggagt aaaaaacaaa gccgatactg
    12841 gtaaccggaa aaaatcatat cttaaccgac taagtttcac tctccataaa ccgctttaca
    12901 cgattcaatt tagcgctctg gatattaata gccagaaaaa ctttttctat gaatttcaaa
    12961 ttgagaccct tcctcccagt agcttgctta gcgatataga attgaacagc gaagttaaac
    13021 ccgatacttc tcaatattta gttaaattta aacgaaataa tgttctctac cgttctgagc
    13081 cctcaattct gttcaacaag tccattaatg attatatcta tctctatctg gaaatctata
    13141 attggaattc cacttcggaa agctgtctgc taaacctttc tttggaaaag gattcgcttg
    13201 tagttatgga tgaatatatt gattttcaac ccggtccagg cagtgaaagc atcaatttaa
    13261 aaatacccct taaagacctg aatcctggct tttataatgg aactattgtc cttcaaagcg
    13321 ccgaacgcac tgaagaaaga aatatagaat ttgcggtgat tgaagaaaaa gaagaggaat
    13381 tttttctgtt tgcaaacccc gatgaagaat atgaactaat gcgtatattt ttaggcaata
    13441 aaataccttc ggactgggga aaaatgaatc aggatacaaa aagaagatat attactcaat
    13501 tctggagaga tatggctttt tctaccaacc gtagtgttca aagcattatg gatttggtgc
    13561 agaaacggat tgattatgca aaccgtaatt ttactcattt taaacagggc tggacttctg
    13621 atatggggcg tatttatatc agaaacggac cgccggatga tattcaacgc gataccagtt
    13681 cagatgaaag ccgttttgtg agaaaggact atcagatatg gaaatatgtt tcagggaaaa
    13741 aaccggtcta tatgttcatt gatattcaga tgaatggcaa ttaccgttta atttatgtag
    13801 aaaatgatga tctggaagct tcaaatcccg attggatgcg ttatgtggga agcgattttg
    13861 atacttccaa actggataat taatactcta aaatagcgtc aaaagcattt tcaaagtgct
    13921 taatctcaaa agtatcggtg ttttcatagt agaaaacgag caaaacatca aagcgaaaaa
    13981 tgtgtttggc aaattgggga ttttcattaa tatataattg tgcagttctg gatatttttc
    14041 tctgtttgga gaaaccaatg gaagccagag cccattctat agagtgagag gtacgggttt
    14101 taacctcaca aaaaacgagg tgttgatctt tttccacaat aatatctatc tcgccatatt
    14161 tcttcctgta attacggcaa gtaatagtat aaccgttact tacgagatag cgagcagcaa
    14221 gatcttcgcc gatgtgagaa aaatcctgaa gtgaatattt tttcataaag caaaaaaaag
    14281 cttgacgatt atattgtcaa taagaatgat gaactttgat aagaaagtaa caccgaaagg
    14341 tggagggaaa aatgaaaaaa actttgttga ccgtaattgc cctggttgct gttcttgggg
    14401 ttgctcaagc agcacctttt caaaccttgg gaatgctaag aacacccgat gcttatgttt
    14461 taccaaataa agctgcagag tttctggtgg taggttatta tcgggatata tcagctccca
    14521 aaccacttac tgaagacgcc aaagatgcct ccaaaggttt ctttccgtat acaatgatag
    14581 gagtaggaat tctggacaga gtggaactta gtatgtttct tggggattac acctctttag
    14641 atggtttagt ttatttactt aatgcaaagg tgaaaattat tgaggagacc ttacttattc
    14701 cccaaattgc cgtaggaatg gataatatct tatctcctgt agaaaagcac ggagctcaat
    14761 acttggacgt aaataatgat tttgctgata atccggaagc tgattcttac gagccctatt
    14821 ctccttatgc agtggcatct aaacaggttg tcttttgggg aataccttgg atgttcaatt
    14881 tagggattgg ctcaaaccgc tttgtgggac aagctccgag agcgcgttat ttaacaggaa
    14941 cctttttctc tgtggagatg tcaccggtca gagatttgac tttgcaggga gaatttgatg
    15001 gtgaagacat caatataggg ataaattatg cctataaaaa ctttggtttt aagctgggag
    15061 ccagtgctat tgaagaccgg ttcaaggata gcgattataa gaataatatc cgtgttgctt
    15121 tagggatttc ctatttgttt gacaaatatg ctgaagctaa acgccgtccc gatcttggtc
    15181 tttatgccag ttatgggaaa aagccatctg ctggcggaga agtagttgag gttggagaaa
    15241 cacctattga aacaggtgta gttgttcctc caacaggtac tactgtaagt cccactggag
    15301 aaattgttgt tgttcctcct acaggaacag cagccaaacc tcctgctgga ggagaagttg
    15361 tagttggtcc tggaacccag ttacagaccc ctggtttagt tacacctgga actgcaggtt
    15421 atcaacagct ttctcctgaa gttcaagatt tgcttaaaga acttcaaatg ctgaaagaag
    15481 agaggcaaaa agctcaacaa gcaatggaag agctgcgcaa atggatagaa gaattgaaga
    15541 aaaagcaata attaatctta aactactcat ctaaaatgtg taggttgagg aatcatgttt
    15601 cccctaaata ctgatgaaga aggttcagga tgcatttgtt aagtgaaaaa atcctgaacc
    15661 ttatcatcat agggaaaata aagagaatat aagtgagcgg gattatgaaa cacaatctta
    15721 aaattatatt cctaattgtt gttgcagtgt cattcttggc agtggtttat gctcaagata
    15781 gatctcaata taccaaagaa caaatccagg agcaaatatc ccaggaaaaa cagctgatca
    15841 gagaattagc caagatgttt ttagttgatg cactgccaga attggtgaag gaagatccga
    15901 cactggctga acaggcactg gtaaaatata aagatgtttt ttccagtatg gatcaaaatg
    15961 aatttattta tttacttggt catttctatg ttcgtgtagg agaaaatgcc aaggctatca
    16021 gtcattttac ttccttgctc aacactcatt tgaatgaaga cgcccgaaaa atgctgaact
    16081 tggttttatt ccagcaaatg gtgcattatt tggagcaggg taaccgaaaa acggcaaagg
    16141 atttcttaag cgctatagta tttgagaatt acaatattga tagatattat cctacttatc
    16201 tatatatatg ggctgatatg gctgctgatg atggtgaata tgaaagtgtt ataaatacgc
    16261 ttaatagtta cattgaaaac cgggatataa taatgaatcg tttgcttccc gaaaaaatgg
    16321 caatcctggc aagagtgcag aacttaaatt tagagcccta ttatgctaat cccagccctg
    16381 ctgaatataa aaggttagct tcccaaatag atggtataaa agctgattta acccgcaatt
    16441 ataacgaatt aatttctttg aaagggattt tttatctgga ttccatcgtt aatctgcatt
    16501 cagaagaaat ggctatgctg gattcactaa agatggttat tcttgattat atagatcaaa
    16561 aagttcaggc tgaggaaaca atggtaaggg gtaatttgtt atttcagcaa gtaaaacaat
    16621 tatcttccaa ctaccaaaaa cagcttgaga taatggataa aattctggaa aagcagtatg
    16681 agcgctttct gactaatgat cccagtgttc aaggtcaaaa ttttagtgat atggaactga
    16741 aacgattatt tgatattgaa aagaacattc agctctataa taatcttatt gctgaactgg
    16801 ataaagatat tgcggatcct gatttagcta atattcagga gcagttgaaa aggaccagag
    16861 cagattattc cgaaaaacga accagtttat tgatcaggaa aaatgacctg ttgcaaactc
    16921 gtaaacatat tagcgatgtg gaagaacaaa tctttaacgc tcttttgcag gaatattact
    16981 ctctctctca ggataaaaaa gatatagatg ttcagctggc tgagattgaa caatttatgc
    17041 aagaagaatc aaaaaatatt ttcacagaag aacaacgaaa taaaatcaaa aaccgtattg
    17101 ctactcaaat agccgtaact gctgatagtg gtgtgcgcaa tgagcccatt cgtaaaaatg
    17161 cccaagattt agttgctaat gtggaatttg ttaaattgca actggattat cgcaacctgc
    17221 atattaagga acaagcacga atcgcgcaaa tagaaaagtg gactgaacaa cagctgactg
    17281 agcggcaggc agaaatcttg gcggaaaagc gtgatttaat tacccgtatt caaaatttta
    17341 ttaacaataa tcccaatttt aaggcagttg aacagccaaa tggaaccttt cttatcagta
    17401 atgctgatct ctattataat ctggctgagc ttcaatatgc tgtttatctg gagaccccta
    17461 ctattgcctt ggaaagttat agaaaagttg ttcagcttga tcccaacaat gtgaatatag
    17521 atgcagccct atataatatt ggttttatca gtagtcagct tagccatcaa aggataggcg
    17581 ataataaagc aaggttttat gaaataaaca ggactgccgt agctcttgat gatgcaagca
    17641 gatataagga gtcagatttt gccgaggcga tcagttccta tcaaagaatt atagataact
    17701 ataagacctc tccttattat gacgagtccc tataccgtct gggaattttg tattattata
    17761 ttgctaccga tgctgatcag ccgcagcgtt actatgcttt agccacaaac tgctttgatg
    17821 aaattattaa caaaccggat agtaaatata aatacgatgc catttatcag cgtggatggt
    17881 tacgcttaaa ttcttttcag gaacaggatt ttatgctggc tctgaacgat tttctaactt
    17941 tgttgaatgc tgttgacagt ggcaagattt ctgatcctat gatcgctaaa gggtaccgtg
    18001 aagatgctat agataatatt gctttctgcc tgattgcttt ggatggaagc gatttctatt
    18061 caccagccaa gggtgtagca gaattgcaaa atattgctgc cagttacaag aatcctgaaa
    18121 ttataagcag cattatagat aaagctgctg caaataagtt gaatttatct actattcaag
    18181 caattgactt tatctggctg aaaatagata taaatcctct tgccctggaa aatcccactt
    18241 tagtggattc cattttacaa ttgtatgcca gcgctcagta tgaactgcgg gaaggtcaga
    18301 atttcgatca aatagctcaa gatttgtatc agaatattat aaccaactat ggcaaaaaca
    18361 ctgcctggta tcaagcaaat atgaataaca gcaacatttc agctcagctg gcagtgatca
    18421 aaaatgctta tgaaaagaga gctattcgcc tgcaaaacga atttcttgcc gatcctgcca
    18481 atgaaaccaa attgcttgct tattatcagc acatagatca atatgctgct tttccggagc
    18541 tgcagggtga aaattatgtt gcctggaaag cccaagcaga taaaaccaaa ctactacttg
    18601 gcaatactct taccgagcgt tccaatttac ccaaacacta tttaacagcg ataagaaacc
    18661 tgcaggaatt taatgcaaaa tatccccaag atgaggattt cttcctgaat gaaggtttga
    18721 tctatactta ttcctttaat atttacaacc tgatgaaaga cctttatggg gaaggatttc
    18781 aacctgagcc aggtttaccc gctaatgcgg atgagctgtt tgctttatta cagtcaaatt
    18841 ctcagcgatt tattagtgtt ctgcgcagtg atgcctataa aaatcctgag cgtgaaattc
    18901 aggcagttaa tattttactt agtttagcag atattcaata tgatcgtgga aaattgcctg
    18961 acgctaaaga gttatatctc tctgctttgg aaaatgaaac tttgattccc gatagaacaa
    19021 aatttaccgt ttacggaaag cttgcgtcaa tagcggaaaa tgagcaaaat tatgcccagg
    19081 cagaaacata ttaccgtaaa gcattacctt atgcggctac tccggatgaa cgggaagcta
    19141 taattaataa catttgctat caaattcaaa atagctttga agcagccgag agtgctggaa
    19201 attattctct ggcagcaagt gaacgattgc gtttggcaga agaacttggt tctaaggatg
    19261 cacaacgtgt taccggttat aaaatgggag ctcaagaggc atatattaaa gcaaaagaat
    19321 atcagaaggc aatagatatt ctattagaac ttgctggaac gcgaacggat attggtgaaa
    19381 tttactatta ttatttccgt gcagcagaaa ttgccgaagc cgatactgca atgaacaatc
    19441 cccaacttgc caaaagcatt cgtgaaagct ttattgctaa atatcccagt agcaatcaag
    19501 cattcaagct acgtatggca atgattcagg aaatggaaaa aaatcctgct cagaagaatg
    19561 ctgctgcaga tgcttatctt gctctttatg aagaagccag aaacaaaact attaataccg
    19621 gaaatattac tcctgacgca ttattagcaa aagcagccat actttacagc gaagctgaaa
    19681 acaaggataa agcagttgaa acctataacc ggttcataac cttatatcca aacagtgaaa
    19741 atgtgatacc ctttatgcag gaaagtgctg attattattt agctaaaggg gatactctta
    19801 agtttgagca actagctaaa gaaatttacc aaaaagacaa aactaagggt gaccgctatc
    19861 aatggattgc tactttaaag ctgagtaaac tggtttataa ttttgataat gcctataaga
    19921 ataagaatta taccgatgcc tttaagtttc gtgatgaata taaaaggttg gaagcagttt
    19981 ataaaaaaga aggactaaat tttgaaacga aggactttag ctctaccaag aattatgaat
    20041 actttgccgc agtggaaaaa gaatataacg atctccagaa acgaatcgct ttcctgaaaa
    20101 attttgacaa ccagcttacc gctattgaaa agggaacttt gatgacatca tctcctgcag
    20161 ctctaatcaa agttaatgcc agcactacct ggcagaatca cttaattgga ggttcctatc
    20221 gtcgtattcc caatttcaag gcatctgtta aagcagaagt agccaaggta aacaagcttc
    20281 ttgccgatgc tgataaacag aatattgaat tggaaaatta tcgccgtctg cgcgcgttga
    20341 acttaattgc ccgcatttat gaaaagggag ttaatgttat taacacccaa attggagttt
    20401 atgtgcgaac tgccagtgaa gtagaaggtg tccgtcaaga gtataaagat gttttagatg
    20461 cttttattgc aactctggct gccgattata gcaatgacct ctttagtttg gcatacacta
    20521 ctcatttaaa tatctataac ttttatcagc tggctggtta taccgactat tatacgcagc
    20581 aaagttatgc caaactgcag gaatggaatt tgttgcccga ttataaaatg gaggaatatc
    20641 ctcttggttc cggctggact gaaaaaatgg atgggacaaa tgtaaattta ccggttcaga
    20701 acataacttc gcctaaaggt gtgcgcttga gttgtgttca aataccttcc aataaagaac
    20761 ttattctaaa tcgcactgtt aatgctaaaa tccaacccga atttgcttta ttgcacttag
    20821 tttatcctta tgatattaaa attcagctga atggcactga tctgaaaccc ggtgttgttc
    20881 ctaccgatac cttagatgtt aataagccga taactacaac ccgtttagca tatttactac
    20941 cttccagcgc ctgggttgaa ggacaaaaca taatagaatt gactgttcct aatgatagtc
    21001 ctgaacagca aagtttatat atgaatttgc aggtctttat agaccgcaac aaattagccg
    21061 cttccatacc tccggaaaca gtgatgcttt atactgatcc tacctggaga attataaccg
    21121 ttaatcctga aacgggagtg gaaacagctt ctgctgcaac tattacaggc aattttggta
    21181 tcaccctgga taacattgta ggactggaag aaacttttgc caaacctatt tggttccccg
    21241 aaacagcacc tgtaagcagt gctatttttg aagttgattt ttacttggat accgagttcc
    21301 gggacggttt aatcaacttt gtagcaccgg aagtagcttc ggtttatcta aatggacagg
    21361 aattagctgc taatcttcat ctggattatg atcctgaacc gtttagggtt tatacatccc
    21421 aggttacgat agacccagca aaagtaactg ctggcaagaa taccttgcgt tttgtagtaa
    21481 ctaacaattc aaactaccgt ggctttattg caacagttaa aattattaaa gcaggaaagg
    21541 gggatatacg atgaaaaaag tttttatcct attagcggtg ttgctctttt ttacaagcat
    21601 tatctatgcc caagcagcaa caacaaccac tcaacagcaa ccggtaaagg gttttgaagg
    21661 tgtccgcaga tatgtggaag aaaaagagtt ggttattgaa ataaaagctc ttatcccatc
    21721 taccgatatt tttcaagata aatccgatac ctatcggaag gcaggggaat ataagttctc
    21781 ctttacaatt gaagatattc ataaacaaag cgatacatta ctaaataaat ggctgaagga
    21841 gtcaaaatga aaacaaaaaa tatcgtcctt atgatcatct cactctttat gtttgttggc
    21901 ctgtttgctc agccagcaac agaaacagca gctccagctg caccagctaa acagggttat
    21961 agcttaattc aagtatttaa agatagcggt aattgggctt atgctatcct gttggttctt
    22021 gttattggtc ttgtttatgc ttttgtgcgt tggtatcagt tatatcccaa agagaagatt
    22081 gatgccgctg gattttattt aaaacttcgc aattatatcc gcaatgacca gctggatgaa
    22141 gcaactaaaa ttgctgaaag ctataaggga actactatgg gctttatctt ctggagcgct
    22201 ttgatggttt tcaaagattc tagaaaaagt ggtctaacgg gttctgattt gagaaattct
    22261 gtccaaaatt ctctggaaga agctgttctg caaacagtgt ataaactgga tagcggttta
    22321 ttctggtttg atactttggc tcaagtagct acttatcttg gacttttagg aaccatttgg
    22381 ggtttgttag gagcatttgc aggtttagct ggtttaactg gtgcagcaca acaaactgct
    22441 ttaactgatg gtattaaaaa agcaattgga acaaccgctc tgggtttgat gaccgctatt
    22501 cctttaaccc taattaaagg ttggttgcta acccgggcaa acaaaattat cagtaatatt
    22561 gacgagttta gtgttaaatt gatcaatact attaacaatg ccattaagga ttaaaaacta
    22621 tgagacaata cagaccatcc gcaatgcgcc agcaaaaatc ccaaacggtg ccccaggaac
    22681 cgaacctggt gccgattatg aatctgtttt taaccattat tccaatgttg ctaatgatgg
    22741 tggttatttc gcagattgct cttttggcgc taaatttctc gcaaggggaa agcggtgtaa
    22801 gttatggaca aggtggcgga ggcggaggtg ctaaaactga aaaagtggaa atccatattt
    22861 tgggtgaaca atatgcccaa gagaataata ctactttagg tatggaaatc cgcgaaccgg
    22921 gctttaagcc gatgactatc tctgccatca atggatatta tgattatgta accctgaata
    22981 aaactctgca agaaattaga agccgaaaag gaactcttag cgatattact gtcctcgttc
    23041 atccggatgt cctttatgga gacctgatga aaacgataga catctgtaaa gtcaatggat
    23101 ttacccaggt tcattatact gccactaaag tggagtatta ttaaaggagt gacttatgca
    23161 aattctggac aaactaaaat cttctaataa aagtgaacgt cgcgtccgta aaaaatttga
    23221 ggaagcaaaa gggttgtcca ttacttcatt gatggatgtg ctaaccatta ttttggtttt
    23281 cctggttaaa aatatctcta cagaagtaat aaaaatttct gcagaaccca atattaccta
    23341 tcctcatacc atcaccaatg ataagctttt agaaaaagcg ggaacaactc cgattaaaat
    23401 attcccagat cgtgttgttg ttggaactga agccctggaa tacggttcgc cggcagattt
    23461 gctaacaaac gcccaaaaac gcaatgacct tttaacttat ctaaaaatgg aagccgagga
    23521 cattattcag catcaaaagg cagagacctg tcttctggtt caagccgact attccattcc
    23581 ctgtgaatat ataacggaaa ttgtccgtgc tgctacagca gcaggttact catacatata
    23641 ttttgccacg cttgaggatg ccgattggct taaaaagtat agtgttagtt tggctgaata
    23701 aaaaggaggg aaaatgtcca aattattaaa cttaactctg cactataaag gcaaaaaact
    23761 ggatcaggtg cgttatggac gcgattttaa gaacaagttc tttatcggtt cggataaata
    23821 cctcttctgg caaatcctgg atgagtcatt tccagaaaag cacctgtttg tcactaaaaa
    23881 aggtgatcag ctttacctgc agttgcctcc tggtgcgaaa gtaagttgtg ctaaagatgg
    23941 agaaccggta gatgaatctt atctgacaaa aagtttcatt ctgcagggta ataaactttt
    24001 acttcgtccc gatatgacag gaactcttgc catcgcacct aattgggaaa tttcttacga
    24061 atttaaagaa ccttatgtaa ctgttcttac tccggaacag caacaaattg ctgctcaata
    24121 tgcctgtcgt cctcaaccta ctgccgatga aaaattcagc cgtaatatga tcctgttgtt
    24181 cgtcattcta accgttattt ttgtgattat ctttgatctg tttctaaaaa aacaggtttc
    24241 ctacgatacg actctggaac agaaacttca gacaattcaa aaagcggaac ggattcaagc
    24301 agaggcatta gcgaaaccca gcacttttga agaagcgggt gaatatgccg aagttcctga
    24361 agaaactgca ggaaaaggac aaccgggtgg agttcccgga ggtaagggaa ccgctaaatc
    24421 agcaggtgca atttttggca gtggctttgg tacttttgat ccgagtgcca cttcagctgc
    24481 aagacctttt caggcagtca ctcttatgga aggatttgtt acttctcgtc ccggaggtgg
    24541 aggtggtgga ggagcaggac ctggtgttgg aggagcagga cctggtggag gtgttggcgg
    24601 aacaactttt gaccccaatg ccactagaag ttttaccagt gatattggct ctgtagcaac
    24661 caaagcgccc acagttaggg gttctgaaat ccgtcccgaa ggggtagcta ttgttaaagc
    24721 ttcaggagat caaagcaaat tgatgcccag tggagttgct tttgggaaaa cagctgtcac
    24781 agaacaaacc cttgcaaagt ttaaaagcaa acaagtaact caagttactg aagaaaccat
    24841 ttctcaagca cctgcagaac gccaaacaat tattgatgaa gttagaagag ctgtggagat
    24901 tcgtaaagga cagatacaat ctctgtatat gaaatggaat gccactattc ctttttcggg
    24961 aagcgtaaaa attcgtctgt taattaacgc ctccggaaaa gtgcaggatg ccataatcac
    25021 ccctgaaggc aatatgccta atggtttttt aaccgaactg gaacaacttt gcgaatcctg
    25081 gagcttccct ataaatgaag aatttgatta cgaattcaaa ttcagattac gcaaa
//
LOCUS       DAOPFL010000015        24957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2034_length_24957_cov_99.043370, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000015 DAOPFL010000000
VERSION     DAOPFL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 24957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24957
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_24957_cov_99.043370"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            644..1090
                     /locus_tag="PLG69_02235"
     CDS             644..1090
                     /locus_tag="PLG69_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42360.1"
                     /translation="MKVKFQYGLAGYTGKADGLVFCYNRRMGRVYARKNISPKLVENH
                     HKMGSITANLHSLLPSTGFKDDLRLYVMRYNGLPENAEKEVYSWVNIYLKMMHQLAKS
                     YPEIDLKTITRDYIYDNDLPCISVKRAVEAGLLPPVTDWELLDRTL"
     gene            complement(1184..2842)
                     /locus_tag="PLG69_02240"
     CDS             complement(1184..2842)
                     /locus_tag="PLG69_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI42361.1"
                     /translation="MNKWILIALTAVLILFGCTANRAVPKNEETEPYTGIVTKVAILP
                     LKTMDSSSRNIQKILTVRDFDYVFTKFPQYSLLNMDEVAKQFKMSGYKDVEDLEIEEM
                     KELSEMIGCDILVMGSINSIRSDEFALSMRFFSAKTEELTPLDFKVTNAREQRWKTLE
                     TTFIAKLDDVVSNEVNKIYNIALNNYANGNYAEAEKNLVFALGLDPDLKDAYYYLGSV
                     YYKQGKLEQAIQNLELNLEKNPQHTQTLSILIDIYEKTNQAAKRLNAMEKLATINENE
                     ELWLTIANLYSEQNNIAKAEEALQKALELNPNYIEAKTRLAFLLYDNNRFDEAIPYLE
                     AIFDKYPENELVSTRLANAYQKANRLDDAIAKYENTIKNNPQNTMAYLSAVNLYRLKA
                     TQTSDPAEVAAINKKAIDILNALITNQPNNAIAYMNMAAIYLSQNKLQDAELYANKAL
                     QKDPTLYLPYIYLATISQSKGTNDYNNFIDLEKQAAKAVGKKASTLKTQRDNARNSAV
                     NNFRKALEYLNNAKARTTDEHAIADINNRIARVNQLINQATATY"
     gene            complement(2975..3688)
                     /locus_tag="PLG69_02245"
     CDS             complement(2975..3688)
                     /locus_tag="PLG69_02245"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPI42362.1"
                     /translation="MRNYFYYDILDNTMREYNFLKELCEEPLCVRAGAQNEGLGRANH
                     IWLSPSGGLWFTFDYENNRSLPSFALYLGFCIHQCLQSLFEPLQGKLQIKWTNDIIFS
                     NRKLGGILCNYLPAKRTYIAGIGLNTNNEIDAELGKFGAISLKDILGFEVSNDELCRL
                     IIKSVEDNCRFMENKKTYIDYCNAHLFGKGRFALLEMGGINIEAEIIEIDSNGALSIR
                     NEKGELIAVHTGSILQFLD"
     gene            complement(3689..4726)
                     /gene="queA"
                     /locus_tag="PLG69_02250"
     CDS             complement(3689..4726)
                     /gene="queA"
                     /locus_tag="PLG69_02250"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPI42363.1"
                     /translation="MPDLLATDTYDYYLPKELIAQFPAEKRTDSRLMQVKRQSREIYH
                     HRFTAILELLKEGDLVIINSSKVFPARLFATKETGAKIEILLLHQLNENTWQCIAKPG
                     KKLKSPQWLHFSDNLSGYISQGNSEGIREIQFTCKTDFWDEIKRIGHIPLPPYIERSD
                     TILDSERYQTVYAEENGSVAAPTAGFHFDAEILNALKKKGVLFSEVILHIGIGTFRPV
                     KTKRITDHKMHSELATIPEITAAAINLAKKEGRRVVCVGTTSVRSVESFWQDGEMQSG
                     SKWTDLFIYPGFNFKVTDALLTNFHLPKSTLLMMISAFGGLETIKKAYNIAVQEKYRF
                     FSYGDAMFIED"
     gene            complement(4726..5493)
                     /locus_tag="PLG69_02255"
     CDS             complement(4726..5493)
                     /locus_tag="PLG69_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPI42364.1"
                     /translation="MKVKFYTIFFFLILMVNLTAMEVPFGEGEKLTFTIKYGLVSAGE
                     ATLEAKSSVYQGSPVWHLSTKAKTYPFFDKIYKVRDTVESWWDKNNLQPYKFSKYLQE
                     GHYRQHRVHIYDHSSLNSTYKKWSFQNQVFENSEMEIPPGTQDLLSAFYLVRTKNLQV
                     GKPIMVNITADGRNMPTEIVVHRKEQQKTIFGNIECLVIEPKLKGEAIFKQSGRILIW
                     VTNDAYKIPVRLESKITIGSFVATLKNAEQVPYKIQI"
     gene            complement(5493..6233)
                     /locus_tag="PLG69_02260"
     CDS             complement(5493..6233)
                     /locus_tag="PLG69_02260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPI42365.1"
                     /translation="MRQILISGANSQIGSYLAKEYYKEGYKLVLLYHKRKERISELFN
                     KDGIFSCPVDLCNSEAVKEAVFRAEQFLHQSSDTLIHCASIRSSDALPLYQTEPMQFA
                     NVLSTNMMSAYNILRWTLPSMQENAFGRVIMFGSDVSQKGLQNGSAYSASKAGIVNLV
                     KSVALEMAKYNIVINAISPAPVETNLEEDYSGEYLNFRKRYFAEYLAQVPTGKLVTKE
                     EIKKVTDLLIDENIKNLNGEEIIIDGGV"
     gene            complement(6230..7429)
                     /gene="coaBC"
                     /locus_tag="PLG69_02265"
     CDS             complement(6230..7429)
                     /gene="coaBC"
                     /locus_tag="PLG69_02265"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPI42366.1"
                     /translation="MSGKKILLCVSGGISAYKTIDLASQLSKEGWEVKCVLTDNALKF
                     VTPINFSAITHNSVHTSLFSDIDPIPHISLVSWADLIVVAPATANIIAKAANGIADDL
                     LSTILVACNKPVLFVPAMNVNMYKSVIVQENLQKLKERGNYILQPATGMLACGIEGEG
                     KYPPNTEVIYAIKTYLNYKTDLQGIKVLLTAGATIEAIDPMRYISNRSSGKMGLAIAR
                     ALALRGAEVTLIYGNIQLDIPYYLKEAVFCESSAKMYEAVITRYAEMDWLICCAAVAD
                     YKPKETILQKLPKQEELNLELVPTIDILAELGKLKKANQRLIGFAAQSDNLIQSAIDK
                     YYRKNLDLICANDIRYAGRDDDELIVQGKIKGYPPLKEDPNINSVVMKGDKFLLAHNL
                     INILKEL"
     gene            7756..8775
                     /locus_tag="PLG69_02270"
     CDS             7756..8775
                     /locus_tag="PLG69_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42367.1"
                     /translation="MTYKKIKSLLFCFLFANCAIMNGLEVPLFLRWKSINTGAYCPNQ
                     TGKEIHWNEFYQIGMGIDSLAYKDLFATMELRSRANFIENHIEIYKFDLSWAKNNWEI
                     TAGSIPWGYGLPNKLYSFAPVSADEDQYSYQATRLNRVYLGYNFAENKIAIDVGGDNH
                     NQVTGKLTYQRQKPFGYFQATEEIRAQDNHWQTPVSISSVNTQRQKGDLVLEAQLAFS
                     FLPNYDVTEKHTTTYALLAGKYKINDLTELFGSGEYLELEPTLMSYQQYRIAFSRKIS
                     VYSLNPFYYLDYYDKSTFHRIGLSWDWHFCQTQRIGLIYYMEGSKLNKAKHNLGIQAN
                     LELGL"
     gene            8772..9506
                     /locus_tag="PLG69_02275"
     CDS             8772..9506
                     /locus_tag="PLG69_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPI42368.1"
                     /translation="MKKPFFIVFLLSIILGSCKFITPPERNTPIICLTFDDQRDGVYT
                     YAFPLMQEYGYRGTCYVNSQYLYGPKNMSLAQIQTLHNDYNWEIGGHSLRHEDLAELT
                     YEEADYTLSQDYWNLYNWGFHPRSFAMPRGKCPLEYYPIITKYYEYLRGCNDYAMHIP
                     LNVQGLGYLAFQSSWTADIYKERIRRGIANGEDLIILGFHAIEEPDNIYGTNCPVSEF
                     AEILRYINHLGLEVLPLSEAVDKLNK"
     gene            9674..9750
                     /locus_tag="PLG69_02280"
     tRNA            9674..9750
                     /locus_tag="PLG69_02280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9708..9710,aa:Arg,seq:ccg)
     gene            9904..11673
                     /locus_tag="PLG69_02285"
     CDS             9904..11673
                     /locus_tag="PLG69_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPI42369.1"
                     /translation="MNEVLLTLEDVSCLYGERIILQGVSFGIHSSEKIGIVGINGSGK
                     TTLLRIISGLIKPNTGTVTMRKDLKVCLLEQDPVYISELSVLQHTFPVAGEIKDEYHY
                     KSILSRLGINNFDEKMGDLSGGQRRKADLARVLAAEPDLLLLDEPTNHLDLETIEWLQ
                     NYLANAGKAVIFVTHDRYFLDAVATKIIEIERTKLFYYEGNYSAYIRGKLIRDTDDKR
                     KETRRQAQLKKELDWLNRGAKARTSKPKDHIDRVKELLSKSYLISHQDLDISFQIDRL
                     GKTILELHRLSKSYSGKVLFTDIDHNFQNMERIGIIGPNGCGKTTLLKIIVGEEKPDN
                     GTVKIGVNTHFAYYKQDEDSFDPNLTVYEYIAQFAEVIKTAEGNKVTATEMLKRFLFD
                     GKMQQMKLGALSGGERKRLYLLKALMFGANFIIMDEPTNDLDIRTLEILEDYLDAFKG
                     CLLIVSHDRFFLDRTVDYLFIFQDGKLRKFAGNYSDYLLVKRFEQDATEEKKEPIITR
                     PKRIAKGLSYNEQREFGLLEKEIEKIESELLELESRLNDFANPLSPNDYYHISIAMQE
                     LENRHQELLTRWLELSDKQNAGN"
     gene            complement(12721..13716)
                     /gene="trpS"
                     /locus_tag="PLG69_02290"
     CDS             complement(12721..13716)
                     /gene="trpS"
                     /locus_tag="PLG69_02290"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPI42370.1"
                     /translation="MKKIALTGIKPTGIPHIGNYLGAIKPALKLSETCEARYFIADYH
                     ALNACKDPVELKKMTVEIASAWLACGLDPINTLFYRQSDVPETFELLTILMAFTPKGL
                     MNRAHAYKATVQNNLEANKDPDDGVNMGLFTYPVLMAADILLFDTDFVPVGTDQFQHI
                     EMAQDIAQSFNYVYKTQALKIPQPIAQEDTKTVVGLDGRKMSKSYNNTIPMFCTEKEL
                     KKLIMSIVTNSQTIEEPKDPDTSNIFALYKNFATPEQVENMRARYLKGGLGWGQAKQE
                     LFEVINAELKPIRERYYYWMNHQEDIWNALKEGSQKARLLASQKIAQLRKIIGID"
     gene            complement(13713..15047)
                     /locus_tag="PLG69_02295"
     CDS             complement(13713..15047)
                     /locus_tag="PLG69_02295"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPI42371.1"
                     /translation="MIKKYTIVSLGCPKNLVDSERFVAIMESYGMIRTDVWEEANILL
                     INSCSFIFNALEELNCLLADVIDATKKDITKIVVTGCVMNRGYEQLKDYYPEVDAWIP
                     LKDFAAFENYLRENILPSLETPKRLSYTQRVHLEDGNYVYLRIADGCNNKCSYCMIPY
                     IRGKQVSEPIETLIEEAKSMQEYGRELILIAQDTCSYGTDIYGKKALPELIEALHKET
                     DYDWIRILYLHPDNFELQWTELWQKFPKLLPYFDIPIQQVSPKIIKAMKRRKSYQELK
                     ELFFHIQQEIPNAVFRTTLMVDYPNETKEDLELLERFLSEVPFLQGGVFAYSPEAKDL
                     KDNIYDEFDWKKSKKLLFAWEDKFYQIRNQLLEKYVGTIQSALIEDYDPYSEQYVGRL
                     WFQAPEIDGCVYIDKLPADNNSLVEVEIVDVIGDEVMSIFHSNINSNKGITK"
     gene            complement(15044..16294)
                     /locus_tag="PLG69_02300"
     CDS             complement(15044..16294)
                     /locus_tag="PLG69_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPI42372.1"
                     /translation="MSTFKPFKALRPIPDKAKAIASLPYDVMDSDEARIEVQKNPLSF
                     LHVEKPEVDLPLGTDLYDPAVYAKARENLYKYVSDGYMKQDTKPAYYIYKQVMDGRAQ
                     IGLVGLTSVDEYMDGTIKKHELTREEKEADRIRHIDACDAHPSPVFFTYRHQNIIDET
                     VAKVMANKQPEYDFISDDGIQHTLWVMDDPEDIKTIQNAFAALPNLYVADGHHRTASA
                     AKVGLKRREQFPDYTGDEEFNFFMAVIFPDNQLKIYDYNRVVKDLNGLTKDEFLAKVA
                     EKWNVTPIPEGENFAPQKKHNISMYLEGKWYRLEPKPGTWNEKNIVEDLDVSILQNNL
                     LQPILGINDPRTDQRIDFIGGIRGLGELVKRVDSGREKVAFAMYPTSMDELIGIADAG
                     EIMPPKSTWFEPKLRSGLFIHLLK"
     gene            complement(16363..16731)
                     /locus_tag="PLG69_02305"
     CDS             complement(16363..16731)
                     /locus_tag="PLG69_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPI42373.1"
                     /translation="MSGFKELKVWQIGIDLVLKVYKITESFPKSEIYGLSSQMRRCAV
                     SIPSNIAEGSSRNNPKEFTQFLYIAQGSLSELDTQFTLAQLLRYIKDFNQIEELIRQI
                     RSMLTGLIKAQKTQNPQTKF"
     gene            complement(16785..18056)
                     /locus_tag="PLG69_02310"
     CDS             complement(16785..18056)
                     /locus_tag="PLG69_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPI42374.1"
                     /translation="MDNLIKDLLAFLSGSKSSFFASLEIRKRLEKAGFTYLPEDKPFK
                     IMRGEKYYLCHQGTTIVAFITGSEKIAKTGFNLAGSHIDYPCLKLKPQSLKTEKGITK
                     IGVQLYGLPIISTWLDRELGIAGKIIVKTAKGYKVEYVDLKKPVAVIPNVAIHLNREV
                     NSGFAYNPQTHLNAILSVSSTGTNPLYSLIAEELKVKEEQIAESDLYLYDFTTPNLLG
                     LKQEMIVSQGLDNLAMTHSILSALLKCEKPQKTAVAIFFNNEEIGSQTSQGANSLLLE
                     EILERICLSQSGSREDFYLALRNSFFISADVANAWHPSFAEKYEPDYAPLMNKGPVIK
                     FDAYNRYASDAESSYRFIQLCEKAKVPYQKFLVRSDATSGVTIGPILSSKLGLKTVDI
                     GNPIWAMHSIRETGGTEDHKYLVQVLEEYYK"
     gene            18238..18690
                     /locus_tag="PLG69_02315"
     CDS             18238..18690
                     /locus_tag="PLG69_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42375.1"
                     /translation="MKVNFKYGIKTYSGTMDEVTYGSYRKNTICIGRKYVKPRLTDQN
                     ALVGGKMKNLAIIYNDVSESYKQELKQYALLNEVNVPKGKLLPNAYALWVKMMFKFAD
                     ADAEHIDLTTLTYSDLQTVGDEILSISSAVENGYMDNVPGADKLTANM"
     gene            18703..18867
                     /locus_tag="PLG69_02320"
     CDS             18703..18867
                     /locus_tag="PLG69_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42376.1"
                     /translation="MNGDNILIKELATRINKLVNIPLINEQNEQAFFELIISILLGIF
                     LDEVDKQLLK"
     gene            19271..19423
                     /locus_tag="PLG69_02325"
     CDS             19271..19423
                     /locus_tag="PLG69_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42377.1"
                     /translation="MEDDEDGMLTGKQYQLLIANLFADDKSSYRDKFYGDSCLRRANK
                     GIFRKK"
     gene            19442..19921
                     /gene="ribE"
                     /locus_tag="PLG69_02330"
     CDS             19442..19921
                     /gene="ribE"
                     /locus_tag="PLG69_02330"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPI42378.1"
                     /translation="MKVMEGKLISKGYKFALVVSRFNEFISSRLLEGALDCLRRHEVN
                     DGDISVIWVPGAFEIPLVAKEAALKKDIDAVICLGAIIRGATPHFEYISAEVTKGIAA
                     VGLETRKPIIYGILTTDSIDQAIERSGTKAGNKGFTAASSALEMLNLLAEMKNGAKA"
     gene            19905..20366
                     /gene="nusB"
                     /locus_tag="PLG69_02335"
     CDS             19905..20366
                     /gene="nusB"
                     /locus_tag="PLG69_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPI42379.1"
                     /translation="MGQRRKAREMAVQCLYSLEFSEVEKDYREYGLLNEYPDILLSLA
                     EAEHIQPNSPVYAFADELVKNTIINIEQVESEIDKISDNWELEDIALLDRSILCLAAY
                     ELLFTDTPAPVVINEAIEIAKKFSSEASGKFINGILDALNKEIIQKGKLTK"
     gene            20370..21278
                     /locus_tag="PLG69_02340"
     CDS             20370..21278
                     /locus_tag="PLG69_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42380.1"
                     /translation="MEKIKCSVGIFAHNEAGNIIPLLEAIENQELRETEISEIIVVSS
                     ASTDGTDQLVSSFAETHKNVKLLTQPKREGKSSAINLFLANAQEDIAVIISADVIPAK
                     QTIEKLVSAFKNPAIGATGGRPVPVNEESNYLGYIVHLLWRLHHRMALIYPKLGEMIA
                     FRKVMDSIPKDSAVDEASIEAIIREKGLKLKYIPSAIIKNKGAENLADHIKQRRRIQN
                     GHLWLKAHQHYKVVSQDSGILLKVVLQELKEKPQTFFKLVGAILLEVYCRLLGSWDYY
                     VKGKNPFAWEIARSTKDLNQPKRERK"
     gene            21281..22363
                     /locus_tag="PLG69_02345"
     CDS             21281..22363
                     /locus_tag="PLG69_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPI42381.1"
                     /translation="MIYLIFRIIGFWLMKNLGFPHLMPMNYTVSLLYTCNSRCNTCNI
                     WKKQAKNLTLDEYQKIFKNIGKSPYWITFSGGEPFLRNDIVDVVTAIYKISHPRIINI
                     PTNGILVQTIIEKTDAIASACPKAQIIVNISIDGIEEQHDKIRNVPGNYKKAIATFNG
                     LKALKHKNLSVGIHTVISKFNVDSFPSIANELMRLKPDSYITEIAEERVELDTMGTDI
                     TPSLVAYKSAIDYLIHRIKNSKFKGMSKVTMAFRIEYYNLVKRIMRDKRQIIPCYSGV
                     ASCQISPDGDIWSCCIKAKSLGNLRKNNYNFRKIWFSPLAEMERRSIHKKKCWCPLAN
                     AAYTNMLLHLPTLFRVSWRSFIHWWS"
     gene            22366..23775
                     /locus_tag="PLG69_02350"
     CDS             22366..23775
                     /locus_tag="PLG69_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPI42382.1"
                     /translation="MVSLQSLHSSPPRSVCEYKASREGRFAWFLLTWHPEKDFVLEEI
                     VAEIKLEILNSKVGSLSRLEIEKWMKQFFADLHWKLYGLLHKTELAEKGISLFFGALY
                     DNELYFVQFGRIFCLLTDNKKMRPIGRDWHNYQVQSLDGLQLLGLKDSDPVIKPQRVY
                     IGSNQRLIVISGELAQKIFPSVDDPSAIETLIETYATEDNPLWLILEGKEELVKTKRR
                     KLSRLQVTSIILIVLALLAALYMMVGNRILDQLVYKMRLSLKEEKTLHLEQIPTNLNI
                     NSENLKKYIDKLINLPARNISFSIAWSTELPYNVSLSPSFTLGTIYLVSQNRLSAYDK
                     KSRQLLWTKMLEDDIRSTTVHQGMLILYLANQQIIGLNDEGNILWQQHLPTESITGDR
                     FIPCIIKNSDDPRLDRSIMVIPSKRGISILDPVRGETISSLTFKHDLDALSVYDSFAN
                     CFYAVVGNAVLCVELKIVN"
     gene            23779..24450
                     /locus_tag="PLG69_02355"
     CDS             23779..24450
                     /locus_tag="PLG69_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPI42383.1"
                     /translation="MKRSLIFFCLLLCLPFLAVVIDNPVKIGFARLQYDGGGDWYNDP
                     EVLPNLAKYANLVMGASFPIDQSVVRASDPKLFDYPFLYLTGHGNIRFTDKEVENLRT
                     WMLRGGFLYADDDYGMDESFRREIKRIFPERELVELDPSFPLFYAFFDFSSGLPKIHL
                     HDGKPPQAFGIFDDNGRLMCLYTYETNISDGWADPETHNDPPEIREKALKFGVNILYY
                     VMHKQ"
     gene            24447..>24957
                     /locus_tag="PLG69_02360"
     CDS             24447..>24957
                     /locus_tag="PLG69_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPI42384.1"
                     /translation="MKIIALSDCHYKFHCASNADKESANKLISFLKSIPGKYDLMVLS
                     GDIFDFWLEGKFTVVKQYFPVLCALSAIKESGTRIVYISGNHDFWFGNFLSDYIGCEI
                     HPDGFTLNTDGKKIRFEHGDTRTFNDLRYQLYRKVIRWKAVHKIASFLHPDLALTIGS
                     RFSRTSRQRK"
BASE COUNT         7563 a         4706 c         4795 g         7893 t
ORIGIN      
        1 tatttgcatt cacaaatttc tcaagcactg gaagaatgaa aaatgaattt tataaggata
       61 tcaagtgaga attgcaaatt gtaaattgag aagtgaaaat tgagaattgc cctgacgggc
      121 tttaatgtat ctaaaccatc taaaccttct caaccatcta aaccttattt tccgaggggc
      181 ttacgccacc atcgctatga aagtgtcgcc cttcggggct ttaatgtatc taaaccctct
      241 aaaccttctc aaccttctaa acctcatttt ccgaggggct tacgccacct tctacatcat
      301 tgtgttgccc atcgtgcttt taaagatctc aaccttttca accattttca attctcaatg
      361 ctgaatactc aattttcaaa tttatcattt ttcaaccttc attaattgtt tgttacatag
      421 tcaattaagt aacgactccg taacacttcc gtaacacttc cgttgtctcg ttacggagtt
      481 gttacttgat tgttactttg ttataactgg ctacagagag caggattcgt ccttcaatta
      541 aaaattattt ttgtccgaat gaaagttttt tggcattttt gtcaccactt cgcatttttt
      601 tccggcgtat atattataga aggacaaaat aaggagaacg aaaatgaaag taaaatttca
      661 atacggctta gcgggttaca caggcaaggc agatggcctt gtattctgtt acaatcggcg
      721 aatgggcagg gtttatgccc ggaagaatat ctcgccaaaa ttggtggaaa atcatcataa
      781 aatgggttca ataacggcaa atctgcattc gttactgccc tcaaccggtt ttaaagatga
      841 ccttcggctc tatgtgatgc gctataacgg tttaccagag aatgccgaaa aggaggtgta
      901 ttcctgggtg aatatctatt tgaagatgat gcatcaactg gcaaaatcgt atccggaaat
      961 agacctaaaa actattaccc gggattatat atacgataat gatctacctt gcatcagtgt
     1021 taaaagggct gtggaagcgg gtttactgcc cccagtaacg gactgggaac tgctggatag
     1081 aactttgtaa agaaggttaa taggtgaaaa ggttagcgag taatttttac ctatttactc
     1141 gctaacctgc caacctacta atatctcaac tgcattattg tgattaataa gtagctgttg
     1201 cttgatttat gagctgatta actctggcaa tacgattatt gatatcggca atagcatgtt
     1261 catcagtagt tcgtgctttg gcattattga gatattccag ggctttacgg aaattattga
     1321 cagcagaatt tctggcatta tcgcgttggg ttttaagagt gcttgctttt ttacctacgg
     1381 ctttagcagc ttgtttttcc agatcaatga aattgttata atcgttagtt cccttgcttt
     1441 gactgatagt agcaagataa atatagggca gatatagggt tgggtctttt tgtaaggcct
     1501 tattagcgta taattcggca tcctgtaatt tattttggct taaatagatt gccgccatat
     1561 tcatatatgc aatagcatta ttgggttgat tggtaatcaa agcatttaag atatcaatag
     1621 cttttttatt gatggcagca acttctgctg gatcggatgt ttgagttgcc tttagacgat
     1681 atagattcac agcacttaaa tatgccatag tgttttgagg attatttttg atcgtatttt
     1741 catatttggc aatggcatca tccagacgat tagctttctg ataggcattg gctaaacgcg
     1801 tagaaacaag ttcattttcc ggatatttat caaatatggc ttccagataa gggattgctt
     1861 cgtcaaaacg gttgttatca tacaaaagaa aagccaaacg cgtttttgct tcaatataat
     1921 tagggttcag ttccagagct ttttgtaaag cttcttctgc tttagcaata ttattttgtt
     1981 ccgaatacaa attagctatg gttagccaca attcttcgtt ttcattgatg gtagcaagtt
     2041 tttccatagc atttaagcgt ttagcggcct gattggtttt ttcgtaaata tcaattagaa
     2101 tggaaagggt ttgagtatgc tggggatttt tttccagatt taattccaga ttttgaattg
     2161 cctgttccaa ttttccttgt ttataataaa cggaaccaag ataatagtag gcatctttga
     2221 gatcgggatc aagacccaaa gcgaagacga gatttttttc cgcttctgca taattgccat
     2281 tggcataatt attgagagcg atattataaa ttttattcac ttcgttggaa acaacatcat
     2341 ccagtttggc aataaaagtg gtttccaagg ttttccagcg ttgttcccgg gcattagtaa
     2401 ccttaaaatc aagcggggta agctcttcgg ttttagcgct gaaaaagcgc attgacaaag
     2461 caaattcatc actccgaata ctatttatac tacccataac gaggatatca cagccaatca
     2521 tttcagataa ttctttcatt tcttcaattt ccaggtcttc cacatcttta taaccggaca
     2581 ttttgaattg cttagctacc tcatccatat tcaaaagaga atattgggga aattttgtaa
     2641 aaacataatc aaagtcacgg acagttaaaa tcttttggat gttacggctt gaagaatcca
     2701 ttgttttaag gggtaaaata gctactttag taacaattcc cgtataaggt tcggtctctt
     2761 catttttagg gactgcgcga tttgctgtgc agccaaagag aataagcaca gcagttaacg
     2821 ctatcagtat ccatttattc atttttcctc cagagtatag caatcgaaat aattttgttt
     2881 aaccaacttt tacaaagagt gtatctgtca agcaaaaccg tttttggctt atagtaaaac
     2941 gatttttatg tgcgtgacaa agaaaaaata tgtttcaatc cagaaattga agaatggaac
     3001 cagtatgcac agcaatcaat tctcccttct catttctgat tgataaagcg ccattgctgt
     3061 caatttcaat aatttctgct tcaatgttaa ttccccccat ttccagtaag gcaaagcgtc
     3121 cttttccaaa aagatgtgca ttgcaataat caatataggt ttttttattt tccataaacc
     3181 ggcagttgtc ttcaacggat ttaataatta aacggcaaag ttcatcattg gagacctcaa
     3241 agcccaaaat atctttaaga gaaatagctc cgaatttacc cagttccgca tcaatttcgt
     3301 tattggtatt taagccgatt cctgcaatat aagttctttt agccggcaga taattacaga
     3361 gaatacctcc taatttacga ttggagaaaa taatatcgtt agtccactta atttgcaatt
     3421 tgccctgcaa tggctcaaaa agggattgta aacattgatg aatacaaaaa cccagatata
     3481 gagcaaaaga gggaagtgac ctgttatttt cataatcaaa agtaaaccat aaaccgcctg
     3541 aaggcgataa ccagatatga tttgctcttc ctaaaccttc attttgagct ccggctctca
     3601 cacaaagggg ttcttcacat aattctttta aaaaattata ttccctcatt gtgttgtcta
     3661 atatatcata ataaaagtaa ttacgcattc aatcctcaat aaacattgca tcgccatagc
     3721 tgaaaaaacg atatttttct tgaacagcaa tattataagc tttcttaatt gtttctaagc
     3781 caccaaaggc agagatcatc attaataggg tagatttggg caaatggaaa ttggttagca
     3841 gagcatctgt aaccttaaaa ttaaaaccgg gataaataaa aaggtctgtc catttggaac
     3901 cgctttgcat ttctccatcc tgccaaaaac tttctacact gcgcacactg gtcgttccaa
     3961 cacaaacaac tcttcttcct tcttttttag ctaaatttat agcagcagcc gttatttcgg
     4021 gtatagtagc aagctcggaa tgcattttgt ggtctgttat gcgcttagtt tttactggac
     4081 gaaaggttcc aatgccgata tgtaaaataa cttcgctgaa aagcacgcct ttctttttta
     4141 aggcatttag aatttcagca tcaaaatgaa atccagcagt aggcgcagca acagagccat
     4201 tttcttcggc ataaacggtt tggtatcttt cactatccaa aattgtatca ctgcgttcaa
     4261 tataaggtgg cagaggaata tgaccgatac gcttaatttc atcccaaaaa tcggttttac
     4321 aggtaaattg aatttctcta attccttcac tatttccctg gctaatataa ccgcttaaat
     4381 tatcggaaaa atgtagccat tgaggagatt tcaatttttt tcccggttta gctatacact
     4441 gccaggtgtt ttcattcagt tgatgcaaga gtaaaatttc tattttagca ccggtttctt
     4501 tggtggcaaa aaggcgtgca ggaaaaacct tactgctatt gatgatgact aaatctcctt
     4561 cttttagcaa ttccagaata gcggtaaaac ggtgatggta tatttctctg ctttgtcttt
     4621 ttacttgcat caaacgcgaa tcggttcttt tttctgcagg aaattgagct attaattctt
     4681 tgggcaggta ataatcgtaa gtatcagttg ctaaaagatc aggcatcata tttgaatttt
     4741 atagggaacc tgctcagcat ttttaagggt ggctacgaaa ctgccaattg ttattttact
     4801 ttccaaacgc acgggaattt tgtatgcatc gttggtaacc caaatcaaaa tcctgccgct
     4861 ttgtttgaaa atcgcttctc ctttgagttt gggttcaatt accaagcatt ctatattgcc
     4921 gaaaatggtt ttttgctgtt ctttccgatg aactacaatt tcggttggca tatttctgcc
     4981 gtcagctgtt atatttacca ttatcggttt tcctacttgc agattttttg ttcgcaccag
     5041 gtaaaaagcg cttaaaaggt cttgagttcc tgggggaatc tccatttcgc tgttttcaaa
     5101 tacctggttt tggaagctcc attttttata ggtagaattt aaagaggaat gatcgtaaat
     5161 atgaacccga tgctgacggt aatgcccttc ctgtaaatat ttactgaatt tgtagggctg
     5221 aagattattt ttatcccacc agctttcaac cgtatcacgc actttataaa tcttatcaaa
     5281 aaagggatag gttttggctt tagtagaaag atgccaaacc ggagaaccct gataaactga
     5341 acttttagct tccaaagttg cttctccggc acttaccaat ccatatttaa tagtgaaggt
     5401 aagtttttct ccttccccaa aagggacttc cattgccgtc aaattcacca tcaaaatcag
     5461 gaagaagaag atagtgtaga atttgacttt cattatacac ctccgtctat aatgatttct
     5521 tctccattga gattttttat gttctcatca atcagcaggt ctgtcacttt ctttatttct
     5581 tcctttgtaa ctaattttcc ggtaggaacc tgcgctaaat attctgcaaa atagcgcttg
     5641 cggaagttta aatattctcc gctgtaatcc tcttccagat ttgtttccac cggagctggt
     5701 gaaattgcat tgattacaat attatatttt gccatttcta aagcaacgct ttttaccaaa
     5761 tttactatac ctgctttgga agcggaatag gcacttccat tttgaagccc cttttggcta
     5821 acatcgcttc caaacattat tactcttcca aacgcgtttt cctgcattga aggtaaagtc
     5881 caacgcaaaa tattataagc actcatcata tttgttgata agacattggc aaattgcatc
     5941 ggctctgttt gatagagagg caaagcatct gaactgcgaa ttgaagcaca atgaattagg
     6001 gtatcggaac tttgatgcag gaattgttct gctctaaaaa ctgcctcttt tacagcttcg
     6061 gaattgcata aatccacagg acaggaaaat atgccatctt tattaaataa ctcagaaatg
     6121 cgttccttgc gtttatgata gagtaataca agtttatatc cctctttgta atattcctta
     6181 gccaggtagc tgccaatctg agaattggct ccacttatta aaatctgcct catagttctt
     6241 tgagaatatt aatcagatta tgtgccagca gaaatttatc tcctttcatc accacggaat
     6301 tgatattggg gtcttctttc aaaggcggat agcccttaat ttttccttgc acaataagtt
     6361 cgtcatcatc cctgcctgca taacgaatat cattggcaca aatgaggtcc agatttttac
     6421 gatagtattt atctatggca ctttgaatta aattatcgga ttgagctgca aaaccgatta
     6481 acctctgatt tgcctttttc aattttccca gctctgctaa aatatctatg gtaggaacaa
     6541 gctccaagtt cagttcttcc tgtttgggaa gcttttggag tattgtttct ttgggtttat
     6601 aatccgctac agcagcacaa caaatcagcc aatccatttc tgcataacga gttataacgg
     6661 cttcatacat tttagcggag ctttcacaaa aaaccgcttc tttcaaataa taaggaatat
     6721 caagctgaat attgccataa atcaaagtaa cttctgcccc ccttaaagct aaagcacgag
     6781 ctattgccag acccattttg ccggaagaac gattactaat gtaacgcata gggtcaattg
     6841 cttcaattgt tgcaccggca gttaacaaaa cctttattcc ctgcaagtcc gttttataat
     6901 tcaaataggt ctttatagca taaataactt ccgtattggg tggatatttt ccttctcctt
     6961 caatgccaca ggcaagcata cctgttgccg gttgtaaaat ataatttccc cgctctttta
     7021 atttctggag attttcctgc acaataacgg atttatacat atttacattc atcgcgggaa
     7081 caaaaagcac cggtttatta caggcaacta aaattgtgga aagtaaatca tccgcaatgc
     7141 catttgccgc tttagctatt atgtttgccg ttgccggagc tacaacaatt aaatcagccc
     7201 aagagacaag tgaaatatga ggaatagggt caatatctga aaacaaagaa gtatgcacac
     7261 tgttatgcgt tatggcacta aaatttatag gtgttacaaa tttcagggca ttatcggtta
     7321 aaacacattt aacttcccaa ccctctttgc ttagctgact cgctaagtca attgttttgt
     7381 aagcagatat gcctccgctt acacagagta atattttctt tccggacatt gtttcctcta
     7441 tatcgttatt tgacttttac tttaatttta attcctgatt tgctgtcaat aaaaaaggtc
     7501 aagaagtcaa gagggtaaga agttaagaga ttaaaaggtc tgaaaaaagg attttgtgta
     7561 aacggagctc acggaaactt ggcataaaga aaaagcggag gaatgaggtc ctctggctac
     7621 acaacaaaaa aggatttgac agatatgggg ctttgttaat aaaagaaata agctaaaaaa
     7681 aagagcaaca caatgaaaga caacaacata catataatta ttaacagata ttacttactg
     7741 ttaaagtgaa atattatgac atacaagaaa attaaatccc tgcttttctg ttttctgttt
     7801 gctaattgtg ctattatgaa cggtctggaa gttcctttat tcttgcgttg gaaatctatt
     7861 aatacaggtg cctattgtcc caaccaaaca ggaaaggaaa tacactggaa cgaattttac
     7921 cagataggaa tgggtattga ttcccttgct tataaagatc tttttgctac aatggaactt
     7981 cgcagtagag ccaattttat agaaaaccac atagaaatat ataagtttga cctttcctgg
     8041 gctaaaaata actgggaaat tacagccggt tcaattcctt ggggttatgg attacccaat
     8101 aaattatatt catttgctcc tgtttctgcc gatgaggatc aatacagtta tcaggcaacg
     8161 cgtttaaata gagtttattt aggatacaat tttgccgaaa acaagatagc aattgatgtt
     8221 ggaggtgata atcataatca ggtaacagga aaattaactt atcaaagaca gaagcccttt
     8281 ggttattttc aagctacaga agaaattaga gctcaagata atcactggca aacacctgtt
     8341 tccattagtt ctgtaaatac gcaaagacaa aaaggagacc ttgttttaga agcacaactg
     8401 gcttttagtt ttcttcccaa ttatgatgta actgaaaagc atacaactac ttatgctctt
     8461 ctggcaggaa aatataagat aaatgactta accgagctat ttggcagtgg agaatatttg
     8521 gaattggaac cgactttaat gagttatcag caatatagaa tagctttcag ccggaaaata
     8581 agtgtttatt ccctaaatcc tttttactat cttgattatt atgataaaag cacctttcat
     8641 agaattggct tatcctggga ttggcatttt tgccaaacac aacgaatcgg attaatatat
     8701 tatatggagg gcagtaaatt gaataaagcc aaacataatt tgggaattca ggcaaatctg
     8761 gaattgggat tatgaaaaag ccgtttttta ttgtgttttt actctctatt atactgggtt
     8821 cctgtaagtt tattactccg ccagaaagga ataccccaat aatttgtctt acttttgacg
     8881 atcaacggga cggagtttat acttatgctt ttcctttgat gcaggaatat ggctatcggg
     8941 gaacctgtta tgtaaattcc caatatcttt acggacctaa aaatatgagt ttagctcaaa
     9001 ttcaaacctt gcataatgat tacaactggg aaattggagg tcactctctg cgtcatgaag
     9061 atttggctga attaacttat gaagaagcgg actatactct cagtcaggat tactggaacc
     9121 tttataattg gggttttcat ccgcgttctt ttgctatgcc gagaggaaaa tgtcctttgg
     9181 aatattatcc tattataact aaatattatg aatacttacg tggctgtaac gattatgcaa
     9241 tgcacattcc tcttaatgtt cagggtttgg gttatctggc ttttcaaagt tcctggacag
     9301 ctgatattta taaagaacgc atccgcagag gtatagctaa cggggaagac ctgataattc
     9361 ttggttttca tgcaattgaa gagccggata atatctatgg aacaaattgt ccggtatcgg
     9421 aatttgcgga aatattacgc tatataaatc atctgggatt ggaagtttta cctctttccg
     9481 aagcagtaga taaactgaat aaatagtgta atcgaagctc gctgaatctt tgcatttttc
     9541 tatcctttta tctttgtgga agaaaggtgt cctccgtcta cacatatttt attaggacta
     9601 aaaacctcct gctctctttt acttcttgat tgacataaaa cccctttgca gaaataatgc
     9661 gttagaaaat tgcgtccctg tagctcagat ggatagagtg gcaccctccg aaggtgaagg
     9721 tcaggcgttc gaatcgcctc agggacgcca aaatgattaa cgggttaaca agtgaagtga
     9781 aaaagtgaaa cccgcgctat aaatagaaag cagtaatata actaattata tatgtataaa
     9841 ttaaatgaag ataaatatct gcgtaatctg cgtgaaactt agcggaatct gcgggaaaaa
     9901 aatatgaatg aagtcctatt aacccttgaa gatgtatcct gtctatatgg cgagagaatc
     9961 attttacagg gtgtttcttt tggcattcat tcttccgaaa aaattggcat tgtaggcata
    10021 aacggcagtg gcaaaacaac tttactgcgt attatcagcg gtttaatcaa gcccaacacg
    10081 ggaaccgtta caatgcgtaa ggatttaaaa gtgtgcctgt tagagcaaga ccctgtttat
    10141 atttccgagc tttcggtttt gcagcatact tttcccgtag caggagaaat aaaggatgaa
    10201 tatcattata aatcaattct cagccgttta ggaataaaca attttgacga aaaaatggga
    10261 gacctttctg gagggcaacg cagaaaagcc gatttggcaa gagttttagc tgccgaaccg
    10321 gatttattgc ttttagatga gcccactaat catctggatt tggagaccat tgaatggctg
    10381 caaaactatt tggcaaatgc cgggaaggca gttatttttg ttactcacga ccgctatttt
    10441 ctggatgctg ttgccaccaa aatcattgaa attgaaagga cgaaactctt ttattacgaa
    10501 ggaaattaca gtgcctacat tcggggaaag ttaatcagag atacggatga taagcgtaaa
    10561 gaaaccaggc gtcaagcaca attaaaaaaa gaacttgatt ggctaaatcg gggtgcaaaa
    10621 gcaagaacca gtaagcccaa agaccatatt gacagggtaa aggaactgct cagcaaaagt
    10681 tatcttattt cccatcagga tttggatatc tcttttcaaa ttgaccgttt agggaaaacc
    10741 attctggaat tgcatcgtct cagcaaatcc tactctggca aagtcctttt tacggatata
    10801 gaccataact ttcagaatat ggaaagaatt ggaattatcg gtcctaatgg ctgtggcaaa
    10861 acaacattgc tgaaaattat cgtgggagaa gaaaagcccg ataacggaac tgtgaaaata
    10921 ggtgtaaata cccattttgc ttattataaa caggatgagg atagttttga tcccaatctt
    10981 accgtttatg aatacatcgc tcaatttgcc gaagtaataa aaaccgctga aggcaacaaa
    11041 gttactgcca cggaaatgct gaaaagattt ctttttgacg gcaaaatgca acaaatgaaa
    11101 ttgggcgcac tttccggagg agaaagaaaa cggctctatc tcttgaaggc actgatgttt
    11161 ggagccaatt ttattattat ggatgagccc acaaatgact tggatattcg cactctggaa
    11221 attttggagg attacctgga tgcctttaaa ggttgcctgt taattgtttc ccacgatcgt
    11281 ttctttttgg atagaacggt ggactatctc tttattttcc aagatggtaa attgcgtaaa
    11341 tttgccggta actattccga ttatttgctg gttaagcgtt ttgaacagga tgcaacagag
    11401 gagaaaaaag aaccgattat tacgcgtccc aagcgcattg ccaagggatt gagctataat
    11461 gaacaacggg aatttggttt gctggaaaag gaaattgaaa aaatagaaag tgaacttctg
    11521 gaactggaaa gccgacttaa tgattttgcc aaccctctct ctcccaatga ttattatcat
    11581 atcagcattg ctatgcagga gctggaaaat agacatcaag aactacttac gcgttggctg
    11641 gaactgagcg ataagcaaaa tgcagggaat tgacagtatt tttttacagg aagcgtaact
    11701 ttatatttta tagtgatttt tgttgccttc cctaattgca ttgcttaccc attgcttacc
    11761 cattacggat ggcattagca atgagtccgt attgctatag taatgcttgc ggggaaaagt
    11821 gttagcaacc aggataacac agctaaaaca aatttacacc gattttttgg ataatttatc
    11881 ccaaataatg cagttgaaat aaggttaatc ttatattcag gtgcattaat tatatattga
    11941 attaaaaatt ccgtaggaat gatagttatt aacgacgggt tttaacccgg cgaaaaatat
    12001 cactaagaaa gagaaaaagt tccgtaggaa cgacagatat tatagaagga ttttaacccg
    12061 gtgaagaata gtcaaatttt gtattttata caatttcctg aatgaaaatt cctttgtcat
    12121 acctgcggaa gcaggaattc atattagttc tgacaaaata actgcatttt caatatggaa
    12181 acaatattag ttgtggaatt ctaatttgct ataaaccaaa cgcatctatt ttgtagagtt
    12241 ctgcgaaatc tttggcaaag aaaaaatttg ccaaccaatt tgtcattaac tgccttattt
    12301 tggtttataa atcatcaatc cggttgctcc ggttatctta gttctatttt tgtttccttt
    12361 agggaatagg gaaggttaat agaaataagt tggggctttg attttacgag gggcttacgc
    12421 caccttctac atcattgtat cgccctgacg ggcttttagg aaaagggaaa gttaaaagaa
    12481 aaggttgatt aggtcttcta tttcccgagg ggctaacgcc accatcgcta tcattgtatc
    12541 gccctttggg gcttttagga aaagggaaag taaaaagaga aggttgatta tgtcttccat
    12601 ttcccgaggg gcttacgcca tcatcgctat cattgtatcg ccctaaagag ttttaatgta
    12661 tctaaaccct ctaaaccttc tcaaccctct aaaccttatt tcccgagggg cttacgcctg
    12721 ttaatctata cctattattt ttctcaactg tgctattttt tgggaagcaa gaaggcgtgc
    12781 tttttggctg ccctctttta gggcattcca gatatcctct tgatgattca tccagtaata
    12841 atagcgttcg cgaataggtt taagttcagc atttataact tcaaaaagtt cctgttttgc
    12901 ctgtccccaa cccaagccac ctttgagata acgagctctc atattttcta cctgttcggg
    12961 tgtggcaaaa tttttataga gcgcaaaaat atttgaggta tctgggtctt taggttcttc
    13021 aattgtttgg ctattggtta caatgctcat aatcaatttt ttcagttctt tttcggtgca
    13081 gaacatcgga atggtgttat tgtaactttt gctcattttg cgtccatcca gtccgacaac
    13141 ggtttttgta tcttcttgag ctatcggttg aggaattttt agtgcttgcg ttttatacac
    13201 atagttgaag ctttgagcaa tatcctgtgc catttctata tgctgaaatt gatctgttcc
    13261 cacaggaaca aaatccgtat caaacaacaa aatatccgca gccattaaaa ccggataggt
    13321 aaaaagaccc atattcactc cgtcatcagg atccttattt gcttccaggt tgttttgcac
    13381 cgttgcttta taggcatggg cacgattcat taaacccttg ggagtaaatg ccataaggat
    13441 cgttagcagt tcaaatgttt ccggcacatc actttggcgg taaaaaagtg tatttatggg
    13501 gtctaaaccg caagctaacc aggcagaagc aatttccaca gtcatttttt tcagttctac
    13561 agggtcttta caggcattta aagcatggta atcggcaata aaataacgcg cttcacaggt
    13621 ctcagaaagt tttaaagcgg gttttatggc tcccagataa tttccaatgt ggggaattcc
    13681 ggttggttta attccggtta aagctatttt tttcatttag ttatcccttt attgctattt
    13741 atattagagt gaaaaatgct cataacttcg tctccgataa catctacaat ttccacttcc
    13801 acgagagaat tattatcggc aggaagttta tctatataca cacagccgtc aatttcagga
    13861 gcttgaaacc ataggcgtcc tacatattgt tcgctatagg gatcataatc ttctattaaa
    13921 gctgattgaa tagttccaac atatttttcc agaagttgat tccttatttg ataaaattta
    13981 tcttcccaag cgaacaacaa ttttttactt tttttccagt caaattcatc gtagatatta
    14041 tctttcaggt cttttgcttc gggagaataa gcaaatacac ctccctgcaa gaaaggcact
    14101 tcggaaagaa atctttccag aagttctaaa tcctcttttg tttcattcgg ataatcaacc
    14161 atcaaagtag tgcggaaaac tgcattaggg atttcctgtt gaatatggaa aaagagttct
    14221 ttcagttcct gatagctttt tcttctcttc attgccttaa tgattttggg gctcacttgt
    14281 tgaataggga tatcaaaata aggcaataat ttaggaaact tttgccagag ctcggtccat
    14341 tgcaattcaa agttatccgg atgcaaatag agaatgcgaa tccaatcgta atctgtttct
    14401 ttatgcaatg cctcaatcaa ttcgggaagg gcttttttgc cataaatatc ggttccgtaa
    14461 gaacaggtat cctgagctat taaaataagc tctctgccat attcctgcat tgattttgcc
    14521 tcttcaatta gggtctcaat cggttcggaa acctgttttc ctctgatata aggaatcata
    14581 caataggagc atttattatt gcaaccgtct gctatccgca aatacacata attgccatct
    14641 tccaaatgaa ctctttgcgt gtaggataac ctttttgggg tttccagaga aggcagaata
    14701 ttttcccgca aatagttttc aaaagctgca aagtccttta acggaatcca ggcatccact
    14761 tccggataat aatctttaag ttgttcgtaa ccacgattca ttacacagcc ggtgacaact
    14821 attttagtaa tatccttttt cgtagcatct ataacatccg ccaataaaca attcagttct
    14881 tccaatgcat tgaagataaa ggaacagctg ttaatcaata atatatttgc ttcttcccaa
    14941 acatcagttc ttatcattcc gtagctttcc ataatggcta caaagcgttc gctatccact
    15001 aaatttttag gacagcccaa ggagacaatc gtatattttt ttatcattta agtaaatgaa
    15061 taaacagtcc actgcgtaat ttaggttcaa accaagttga tttgggaggc ataatttctc
    15121 ctgcatcagc aattcctata agttcatcca tacttgtagg atacatagca aaagcaactt
    15181 tttcccttcc gctatcaact cttttaacca attcacccag acctctgatt ccaccgataa
    15241 aatcaattct ttgatcggtg cgcgggtcat tgatacctaa aatgggttgc aaaagattat
    15301 tctgtaaaat ggaaacatcc aggtcttcca caatgttttt ctcgttccag gttcccggct
    15361 tgggttctaa acgataccat tttccctcaa gatacatact gatgttgtgt ttcttttgag
    15421 gagcaaaatt ttctccttca gggatggggg taacattcca tttttcagct actttggcta
    15481 aaaattcgtc tttggttaaa ccgttcaggt ctttaaccac ccgattataa tcataaatct
    15541 tcaattgatt atccggaaag ataaccgcca taaaaaagtt gaattcttcg tcgcctgtgt
    15601 aatcgggaaa ctgctcgcgc cttttcaatc caacttttgc cgcgctggca gttctgtgat
    15661 gtccatctgc tacatacaaa ttcggtaagg cggcaaatgc attttgaatg gtctttatat
    15721 cctcaggatc atccatcacc caaagtgtat gctgaatacc gtcatcgctt ataaaatcat
    15781 attctggctg tttattagcc ataaccttag ctacagtttc atcaataata ttttgatgac
    15841 gataggtaaa aaacacagga gaaggatgtg catcacaggc atcaatatgc cgaatgcggt
    15901 ctgcttcttt ttcttctctt gttaattcat gctttttgat agtgccatcc atatattcat
    15961 ctacggaagt aaggcctacc aaaccaattt gagcacggcc atccataact tgtttataaa
    16021 tgtaataagc cggttttgta tcctgtttca tatagccgtc ggaaacatat ttatagaggt
    16081 tttctctggc tttggcataa acagctggat cgtataaatc ggttcccaag ggaagatcaa
    16141 cttccggttt ttctacatgg agaaagctta aaggattttt ttgcacttct atgcgtgctt
    16201 catctgaatc cataacatca taaggcaaag aagcaattgc ctttgctttg tccggaatgg
    16261 gacgcagggc tttgaagggt ttgaaagtag acatttatat actcctttat tttttagtcg
    16321 agaggtcgag aggtcgagag gtcttgaggt ctgaggacat cgttaaaatt ttgtttgagg
    16381 gttttgtgtc ttttgtgctt tgataagacc tgtcaacata cttcttatct gtctaattaa
    16441 ctcttcaatt tggttaaaat ctttaatgta acgaagtagt tgagctaatg taaattgagt
    16501 atccagttct gaaagtgaac cttgtgctat atataagaat tgggtaaatt ctttggggtt
    16561 atttctactt gaaccttctg caatattaga aggaattgat actgcacatc gtctcatctg
    16621 tgaagataat ccataaattt cacttttagg aaatgactct gttattttat ataccttcaa
    16681 gaccaaatca attccaattt gccagacctt tagttcttta aatccgctca ttttttctcc
    16741 ttcttttttt atttctgacc tctcgacctc ttgacctctc gacctcattt ataatattcc
    16801 tctaaaactt ggacaagata tttatggtct tcagttcctc ctgtttcccg gatggaatgc
    16861 attgcccaaa ttggatttcc gatatcaact gtcttcagcc ccaattttga agataaaata
    16921 ggaccaattg taactccact ggtggcatcg ctacgcacta aaaacttttg ataaggaact
    16981 tttgcctttt cacaaagctg aataaagcga taggagcttt ctgcgtccga ggcgtatctg
    17041 ttataagcat caaacttaat aactggtccc ttattcatca aaggagcata atccggctca
    17101 tatttttcag cgaaagaagg atgccaggca tttgctacat cagcactaat aaaaaagctg
    17161 ttgcgtaaag ccaggtaaaa atcttctctg gaaccgcttt gactgaggca aattcgttct
    17221 aaaatttcct ccagaagtaa ggaatttgct ccctgagagg tctgcgaacc gatttcttca
    17281 ttattaaaaa agatagctac tgctgttttt tgaggtttct cacatttaag aagggcggaa
    17341 agaatagaat gagtcatagc caggttatca agaccttgag aaacaatcat ttcctgtttc
    17401 aatcccaata agttcggggt tgtaaaatca tacaaatata agtcactttc ggcaatctgt
    17461 tcttccttaa ccttaagttc ctccgcgata agagaataaa gtggatttgt gcctgtagaa
    17521 ctaacactga gtatagcatt taaatgggtc tgcggattat aagcaaaccc actatttact
    17581 tcccgattca aatgtatggc tacattggga ataacggcta ctggcttttt taagtccaca
    17641 tattccactt tataaccctt tgccgttttt actataattt ttccggcaat tcccaattcg
    17701 cggtctaacc aagtgctgat aataggtaag ccataaagct gaaccccaat tttagtgatt
    17761 cctttttcgg ttttcaaact ttggggtttg agctttagac agggataatc aatgtgactt
    17821 ccggcaaggt tgaaacctgt tttggcgatt ttttcactac ctgttataaa agcaacaata
    17881 gtagttccct gatgacagag ataatatttt tcgcctctca taatcttgaa aggtttatct
    17941 tcaggaagat aggtaaaacc tgctttttcc aaacgcttcc gaatttccaa actggcaaaa
    18001 aaactgcttt tactgccaga gaggaaagcg agtaaatcct ttattaaatt atccattatc
    18061 taaactccaa tatttattta ttttaaatgc cagattttta atctccgctt tcgtgtcaag
    18121 aagggcaatt tgagaaaaaa tccaaatttg tcaccacctt tcatttttgt caccacctat
    18181 ataagtgaag gcaagatttc cggaaacgcc taaaacaaaa aacaaaggag aaacaaaatg
    18241 aaagtaaact tcaagtacgg aatcaaaacc tactccggca ctatggatga agtaacctac
    18301 ggcagttatc gtaaaaatac tatctgcata ggccgtaagt atgtaaaacc ccgtcttacc
    18361 gaccaaaatg ctttagttgg cggcaagatg aaaaacctgg caattatcta taatgatgtt
    18421 tcagagagct ataaacaaga gctgaaacaa tatgcccttc taaacgaggt aaatgttcct
    18481 aaaggcaaat tgcttccaaa tgcatacgct ctttgggtta aaatgatgtt taagtttgcc
    18541 gatgctgatg cggaacatat agacctgact actctaacct attctgatct gcagacagtt
    18601 ggagatgaaa tcctttctat ctcttctgcc gtggaaaatg ggtatatgga taatgttccc
    18661 ggtgcggata aactgaccgc taatatgtaa aggaggtaaa taatgaacgg tgataatatc
    18721 ctcataaaag aactcgctac ccggattaac aaattggtaa acatcccttt gatcaatgag
    18781 cagaatgaac aggcattctt tgaactgata atctctatcc tgctcggtat cttcttggat
    18841 gaagtggata aacagttact caagtaacat aaattcctaa ccatctgtaa aagtgccccg
    18901 gtttttgccg gggctttttc tgtgcccttt ttatttccgt ctgctggtgt aaactttcct
    18961 cggtaaagaa agcaggtaaa aaaggaaaag agggaggagg aagaattatc aataataatc
    19021 tgtaccccta acgggaactt aatttttctc tgtgctatcc gggaaataga ggagagataa
    19081 aattgaagct cggtaagaag cataagcacc actaaacttg gagtcggaat tataatggga
    19141 taggagctaa gaacttcttg cttacaagga gctaatgctt actaatttgc caggagaatt
    19201 cgttcctccg gaagattttc cttgaacaaa ttataggagc tgaaataata ggttttaagg
    19261 aaaaattagg atggaagatg atgaggatgg aatgctgaca ggcaaacaat atcagctctt
    19321 aattgctaat ttatttgcag acgataaaag cagttatagg gataaattct atggggattc
    19381 ctgcttgcgc agggctaaca aagggatttt taggaaaaaa taatctacaa aggagccaaa
    19441 gatgaaagtt atggaaggaa agcttatttc caagggctat aaatttgctc ttgtagtcag
    19501 tcgttttaac gaatttatca gttcccgatt gctggaagga gctttagatt gcctcaggcg
    19561 tcacgaagta aacgatggag atatcagcgt aatatgggtt cccggtgctt ttgaaattcc
    19621 tctggtggca aaagaagccg cattaaaaaa agatattgat gccgtaatct gtttgggagc
    19681 aataattcgg ggtgcaactc cccactttga atatatcagt gcagaagtca ctaaaggcat
    19741 tgctgcagtc ggtttggaaa cccgcaagcc gattatttac ggtattttaa caacggatag
    19801 tattgaccaa gcaatagaac gctctggaac caaagccggt aacaagggtt ttacggcagc
    19861 cagttctgct ttggaaatgc ttaatctttt agctgaaatg aaaaatgggg caaaggcgta
    19921 aagcacgaga aatggctgtt cagtgccttt attctctgga attttcagaa gtggaaaagg
    19981 actatcggga atatggtctt ttgaatgaat atcctgatat tctgctttct ttggcagaag
    20041 cggaacatat tcaacccaat tccccggtct atgcttttgc ggatgaactg gtaaaaaaca
    20101 cgattatcaa tattgaacag gtggaatcgg aaattgataa aataagcgac aattgggagc
    20161 tggaagatat tgccctgctt gatcgcagca ttttatgcct ggctgcctac gaacttttgt
    20221 ttacagatac tccggcacca gtagtaatta atgaggcaat tgagattgcc aagaaattca
    20281 gcagtgaagc atccggtaaa ttcatcaacg gcattctgga tgctttaaac aaggaaataa
    20341 ttcaaaaggg aaaactaaca aaataggtaa tggaaaaaat aaaatgttct gtcggaatat
    20401 ttgcccataa cgaagccggc aatatcattc ctttactgga agcaattgaa aatcaggaat
    20461 tgcgagagac cgaaatcagc gaaataattg ttgtttccag tgccagcacc gatggaacag
    20521 accaactggt aagctctttt gccgaaactc ataaaaatgt aaagcttctc actcagccaa
    20581 aaagggaagg caagtcctct gctattaatc tttttttagc caatgcccaa gaggatattg
    20641 ccgtaattat aagtgctgat gttattcctg ccaaacagac catagagaaa ttggtttccg
    20701 cttttaaaaa tcctgcaatt ggggcgacgg gaggtcgtcc tgttccggta aatgaggaaa
    20761 gcaattatct gggttatata gttcatcttt tatggcgttt acatcatcgg atggcattga
    20821 tttatcccaa attgggagaa atgattgcct tccgtaaagt gatggatagt attcctaaag
    20881 atagtgcagt tgacgaagcc agcattgaag caataataag agagaagggc ttgaaactta
    20941 aatacatacc ttctgccatt attaaaaaca aaggagctga aaatctggca gaccatataa
    21001 aacagcggcg tcgtattcag aacggtcatt tatggctgaa agcgcatcag cattataaag
    21061 ttgtttctca ggatagcggt attttattga aagttgtttt acaggagttg aaagaaaagc
    21121 cccagacctt ttttaaattg gtgggagcta ttcttctgga agtttactgc cgtctgcttg
    21181 gcagttggga ttattatgtg aagggaaaaa atccctttgc ctgggaaata gcgcgctcca
    21241 caaaggactt aaatcaaccg aaaagggaaa gaaaatagaa atgatttacc tcatatttcg
    21301 cataattggt ttctggctga tgaaaaacct gggttttcct catctgatgc caatgaatta
    21361 tacggtcagt ttgctttaca cttgcaattc gcgttgtaat acctgtaata tttggaaaaa
    21421 gcaggcaaaa aatttaacct tggatgaata tcagaaaatt ttcaaaaata taggcaaatc
    21481 gccatactgg attactttca gcggaggaga accattttta cgcaatgata ttgtagatgt
    21541 ggtaaccgct atctacaaaa tcagtcatcc ccgcattatt aatattccca caaacggtat
    21601 tttggttcaa accattatag aaaaaacgga tgctattgcc agtgcctgtc ctaaagctca
    21661 aataatagtt aatatttcca ttgacggcat agaagaacag cacgataaaa tacgcaatgt
    21721 tcccggaaac tacaaaaaag caattgctac tttcaacggt ttaaaagccc tgaaacacaa
    21781 aaacctcagc gtaggcattc acactgttat ttccaagttc aatgtagata gttttccctc
    21841 tattgccaat gaattgatgc gtcttaagcc cgatagctac attactgaaa tagcggaaga
    21901 aagagtggaa ctggatacga tgggaacaga tataactcct tccttggttg cctataaaag
    21961 cgctattgat tatcttattc atagaatcaa aaacagtaaa tttaaaggaa tgagcaaggt
    22021 tactatggct tttcgcattg aatattacaa tctggtgaaa aggattatgc gtgataaaag
    22081 gcaaattatt ccctgttatt caggagttgc ctcttgtcag atttctccgg atggcgatat
    22141 ttggagctgt tgcattaaag ccaagtcctt gggaaacctg cgaaaaaata actataactt
    22201 tcgtaagatt tggttttctc cgcttgccga aatggaacgt cgttccattc ataaaaagaa
    22261 gtgctggtgt cctttagcca atgctgccta cactaatatg ctgttacatc tgccaactct
    22321 ttttcgtgtt tcctggcgca gttttattca ttggtggagc taaaaatggt ttctttacag
    22381 agtttgcact cctctcctcc ccgtagcgta tgtgaatata aggcatcccg tgaaggacgc
    22441 tttgcctggt ttttgcttac ctggcatcca gagaaggatt ttgtgctgga agaaatcgtt
    22501 gccgaaatca agctggaaat tcttaattcc aaagttggct cgctttcgcg tctggaaatt
    22561 gagaaatgga tgaaacagtt ctttgccgat ctgcattgga aactctatgg actattgcat
    22621 aagaccgaac tggcagaaaa aggtatttct ctcttttttg gcgccctcta tgataacgag
    22681 ctttattttg ttcagtttgg cagaatattt tgtcttttaa ccgataacaa aaaaatgaga
    22741 cctatcggaa gggactggca taattatcag gttcagtcat tggacggttt acaactgctt
    22801 ggtttaaagg acagtgatcc tgttattaag ccacaacggg tgtatattgg cagtaatcaa
    22861 cgattgatcg ttatttcagg ggaactggca caaaagattt ttcccagtgt tgatgatcct
    22921 tctgccatag agacattaat tgagacctac gctacggagg ataatcccct ttggctgatt
    22981 ttggaaggta aagaggaatt ggttaaaaca aagcgcagaa agctttctcg tttgcaggta
    23041 accagtatca ttcttattgt tttagctttg ttggctgctc tgtatatgat ggttggaaat
    23101 cgtattcttg atcaactggt gtataaaatg cgtctatcac tcaaagagga aaagacctta
    23161 catttagaac aaattcctac caatctgaat atcaatagcg agaacctgaa aaaatatata
    23221 gataaactga taaatctgcc cgcccgcaat atttctttct ccattgcctg gagcacagaa
    23281 ctaccatata atgttagttt gtcaccttct tttacattag gcacaattta tctggtatct
    23341 caaaaccgac ttagcgctta cgataagaag tcccgtcagt tgctctggac taaaatgctg
    23401 gaggatgata ttcgctctac aacagtccat cagggaatgc tgatcttgta tctggcaaat
    23461 cagcaaatta tcggtctgaa tgatgaggga aatattctct ggcaacagca tctgcctaca
    23521 gaaagcataa ccggtgatcg gttcattccc tgcatcatta aaaacagcga tgatccacgt
    23581 ttggatagaa gcataatggt tattccttcc aagaggggaa tcagcattct tgatccggtt
    23641 cggggtgaaa ccattagcag tttaactttt aagcacgatt tggatgctct ttccgtttat
    23701 gacagctttg ccaattgttt ttacgcggtt gtaggcaatg ctgttttatg tgtagaatta
    23761 aagattgtga attaagctat gaaacgctct cttatttttt tctgcctgtt actttgtctg
    23821 ccttttctgg cagttgtaat tgataatcct gttaaaattg gctttgctcg tttgcaatat
    23881 gacgggggag gcgattggta taacgatccc gaagtgcttc ccaatctggc aaaatatgcc
    23941 aatttggtta tgggtgcaag ttttcctatt gaccaaagcg tagttagagc ttccgatccc
    24001 aagctttttg attatccttt tctttattta accggtcacg ggaatatcag gtttacggat
    24061 aaagaagtgg aaaacctcag aacctggatg ctccgaggcg gttttttgta tgccgatgat
    24121 gattatggta tggatgaatc cttccgcaga gaaataaaac gcattttccc agaaagagag
    24181 cttgtggaat tagacccttc ttttccccta ttttatgcct tttttgattt cagtagcgga
    24241 cttcccaaaa ttcatttgca cgatggaaaa cctccccaag catttggtat ttttgatgat
    24301 aacggacgct tgatgtgttt atatacttat gaaaccaata tcagtgacgg ttgggctgat
    24361 cctgaaacgc ataatgaccc acccgagatc agggaaaaag ctcttaagtt cggtgtaaat
    24421 attctttatt atgtaatgca caaacaatga aaataattgc tctttccgat tgccattata
    24481 agtttcactg cgccagcaat gccgataaag aaagcgccaa caaactaatc agttttctta
    24541 aaagcattcc gggaaaatat gatttaatgg tgctttccgg ggatattttt gatttctggc
    24601 tggaaggaaa atttaccgta gtgaaacaat attttcctgt cctttgtgcc ttatccgcta
    24661 tcaaagaaag tggaactcgc atagtttata tcagtggcaa tcacgatttc tggttcggaa
    24721 actttttatc cgattatatt ggttgtgaaa ttcatcctga tggctttact cttaataccg
    24781 acggcaaaaa aatccgtttt gaacatggcg atacccgcac ttttaacgat ttgcgttatc
    24841 agctttaccg taaagtaatt cgctggaaag ctgtccataa aattgcctct ttcctgcatc
    24901 ctgatttggc tttaactatt ggtagtcgct tttcccgcac cagcaggcaa agaaaag
//
LOCUS       DAOPFL010000016        24672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2059_length_24672_cov_86.231872, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000016 DAOPFL010000000
VERSION     DAOPFL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 24672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24672
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2059_length_24672_cov_86.231872"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLG69_02365"
     CDS             complement(<1..268)
                     /locus_tag="PLG69_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPI42385.1"
                     /translation="MTNVAINGFGRIGRLVLRVILDSHPELNVVAINDLTDAKTLAHL
                     FKYDSVHKIYPGNVSSEENYLVVNGKKFRIFAEKDPEVLPWKDLG"
     gene            complement(290..832)
                     /locus_tag="PLG69_02370"
     CDS             complement(290..832)
                     /locus_tag="PLG69_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HPI42386.1"
                     /translation="MNIVYEYSHLGGLEILLVRYPKIMEEVLNIIKDIKPHKTKISKE
                     KTMSGKSLFSPKDINKQFKDKFQLLGYKELRDTFSIELPNYEPKITGCYKQIDFCLGK
                     VLVEVQLGKYPFMFYDLAKFQYFFNENKADVGIEIVPCYQLHKSMSTGVSYGEQLIND
                     IERLKRHFPAVPVAIYLIDI"
     gene            complement(829..1761)
                     /locus_tag="PLG69_02375"
     CDS             complement(829..1761)
                     /locus_tag="PLG69_02375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPI42387.1"
                     /translation="MVKISNKWNSDSILCLFQGDCLELLDSIPDAAIQLVVTSPPYNI
                     GKKYEKRQPLDEYIDWQKKVITECCRVLKKSGSICWQVGNYIENGEIIPLDILLYPVF
                     AESGLKLRNRIIWHFGHGLHSSKRFSGRYEVILWFTKSDEYIFNLDPVRIPQKYPNKK
                     YFKGDKKGELSCNPLGKNPSDVWNIPNVKANHIEKTIHPAQFPVELVERLILSMTNEN
                     DWTLDPFMGTGTTQIASLIHNRKSCGAELLDEYYEIALQRINSAISGELKIRPMNRPI
                     FDPEHPISFKPITVNLTKIKEEQSELDLVWSNEK"
     gene            complement(1767..2741)
                     /locus_tag="PLG69_02380"
     CDS             complement(1767..2741)
                     /locus_tag="PLG69_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPI42388.1"
                     /translation="MFLTGTQLKEVFLKAKKQRFGIIASNVVFDTQVRALIQGYAAVN
                     SDGLMQMSTGACKYAAGTSQDLHIGAKMISAMVKIFSAQFPKCGIGLHIDHATPNYFD
                     FIKFCIENDLVSSVMIDASAEKLEDNIRITKEVVDLAHKYNILVEGEIGHIKGTEDEI
                     VSEDELYTKPEEALEFVIKTNVDLFAASVGTNHGVTKGENVVLHLDLIKEIDDLLIKN
                     GAERGLVLHGASGLTDWQQQTAIANGVVKINKDTHYQMDMASAIQEYWEKEKYAIVCP
                     PDVDPAAYIPNKSKFDPRKWLIKGEEKMQNTVMEFCKMSGSANNSILL"
     gene            complement(2880..>5398)
                     /locus_tag="PLG69_02385"
     CDS             complement(2880..>5398)
                     /locus_tag="PLG69_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPI42389.1"
                     /translation="SAISESAATTYSYSLNTTYLIVVKYVIVSGATNDEVYMWVNPVI
                     GETEPAPQLTAADLTGTDIGAIGAVAIRQGSNGPIARIDGIRVTNNWAKLWEGSPMET
                     PVIHTSTTELDPLESIVNLPSDETRDYTLWGTNIVGGITVTAPNGFQVSTSQIEGWAS
                     SITVPADFHSQIYVRMLANAIGEYEGYIVHTSGDAEPVNVRVTGAALPPPVTWNITAN
                     LTPFSSEAGTPSAVQSYTLSAPGAIDSLQITTSYPFELSSTGTGNWGISLTLNNNFSG
                     NVYVRMNATGSGTFNEIIYHNTTYANEYQLPVSGTATSQPGMASDLFFSEYIEGTSNN
                     KAIEIFNGTGGPVDLSNYKVCLYTNGAATPLYTLNLTGTLAHLDVYVIANASANATIL
                     GLADTTSTVTYYNGDDALLLYKKVGTDSTRIDVIGTIGTDPGTAWNVAGVTNATAEHT
                     LIRKPTITQGNLDWAISAGTNADDSEWIVYAQDYTDNLGMHTFGNMVVMPVFNPPAGA
                     YATPINVTISTTTPGAVIHYTTDGSEPTEASPVYSAPIPVSSDTTIKAKGYATGYSPS
                     AIATAFYDFPENVSTIAQLREGTLGSAYRLTGEAVLTFQQANRNQKYVQDATAAIVID
                     DPNGKITTTYNLYDGITGITGTLSSYSGLLQFVPIADPGAATSHNNTIVPVTRTLATI
                     TSADQAKLLKIYSVTLTPNATGVFAATAENINASDASGTGVMRTFPNADYAGTAIPTD
                     PVDIVCLGGQYNDTMQFSPRFLADITPAAGILEAPIVNISQVAGMINLSWNQVPGATN
                     YRIESADDPYGTWSTVTTTPNLFYSGAAVSKKFYRVIATN"
     assembly_gap    5399..5498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5499..6742)
                     /locus_tag="PLG69_02390"
     CDS             complement(<5499..6742)
                     /locus_tag="PLG69_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42390.1"
                     /translation="MRKSLFLILLTLSFIGGLFAQVNIQSGQNVTQNFDVLGTSAAAT
                     IPTGWKADKNTTVRSVGTYSAAGTVTELIAGNNMSSTAQNGIYNYGAGPAATATDRAI
                     GFISSGSATKSGNVYVQLTNNGSTNINSFSISYNVEKYRMGTNAAGFAIQMYYSADGA
                     TWTSAGSSFLTSFAGSDASNSGYDSAPGATVPVSATLSYTLAAGSSVYLAWNYSVTNG
                     STTSNAQGLGIDDVSITAEGAPTPVIVVSGTLTPFSTYTGTPSTAQSYTLSGQNLTGN
                     ITVAAPTGFALSTDGTTYTSNLSLPATFNGLVYVRLTGAAAGNFSGNIVHSSTGATNV
                     NLAVEGTVTDPTPTINITANLNPFGTQLGTPSAAQSYTVTGLFLTAGISVTAPEGFAL
                     STDNTTFTNTLTLASNFSGLVYV"
     gene            complement(7213..10023)
                     /locus_tag="PLG69_02395"
     CDS             complement(7213..10023)
                     /locus_tag="PLG69_02395"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HPI42391.1"
                     /translation="MKKLIIFMLLACSCLFLLADIEDIQLDIGEQNIILGEQTTLPVQ
                     TSSLSGYGVISYELNIAYDTQKLTFLEIVKTNTLSANGMISVNEVNGILHIGAIFTNP
                     LYGQGNLLNLKFSAQNPGLAEVNFVSAVMNTTSLMNLYGGSIYIENQPISASLSVGNY
                     LLQTGSSFSLPIIATGLAGMNCISFQFKFRFDPAVINVISVNNSGSSSEGWEINNRTS
                     SGLISVAGMGTMPITADGTLIFIDIEVLPTAITFSHLQLETVLFNTQPAVSVVDGRLD
                     VYNLWNLHIEGESDIMAIHNNNPLFIWNVINSSATPFECEFQAGSDDNWEIAEYYASG
                     WIENYTSQQLLNLVYGTALFARIRLRIGDFISPWQELAGHLIPLPGSAVCLSPLPNAL
                     NVILNPVLSWQSGLGDPPLGYRLYFGTNTPPSNIVNGTDLGLVTTWQPSSPLQYNTVY
                     YWQIIPYNTYGEPSSCPIWSFTTHDANAIVQYPYLKDFYDGSVTSGGWTTFYSGSAGS
                     NWRINTTNGISGSKCITVGRAPGTYWYISPAVINPNCGSALSFYIKDYSSNSTYDIVG
                     ENLEIMLSTTGSTPQNFTHCLLSLDNFAVTTSYISFTVDLTQFASQSIYLAFKRVSNN
                     GNYLFIDNIQLEQYIPQITLNTSVLVFPTLKPGQTANANFSISNSGTGILSGNLIYPE
                     GFFGVNSFSGNSNTIEVSYHPWSTGGGSYPLQITSSGGNTILYLQANCGEEIQPCESL
                     TNSGYIDNNWQLSSAEKHNGSSSWTTISPEMYLISSWMQGGNGKGFGFWAKANSASSI
                     AIYLNSTGYAIEQNYLYHSNIPLDNNWTYHRISFSDYIDLSVSVKLVCDSGIFIDDID
                     MSNFLAPQTPEVLITKTETVPTISWLPTFNCLSYNVYASSNLTDWSLISPANFQETLW
                     VDNSQLEHRFFKVCGVNGKISE"
     gene            complement(10042..15168)
                     /locus_tag="PLG69_02400"
     CDS             complement(10042..15168)
                     /locus_tag="PLG69_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPI42392.1"
                     /translation="MKELTFKFSFTLFLLCLCGFLMASVAEYSFSSSLGSFSEITNGT
                     VLGSNLNDNECFLSIPIGFTFSYNGNSYTTISIASNGFLAMGDNVTTSNVALSSETAT
                     NNVIAVMNRDLISRDNGSLMTKTIGTDSNRIFIVQWMHYRRRPTATLNDDLSFQIQLY
                     EGSNQVVFVYGPCTAVTSSTSQSIQVGLRGNSNTDFCNRATATDWSATTAGTENNSYC
                     ILSETVFPANGLTFTWNPPSLIEPPFPAVNPFPADLAENVEINTMLSWNSGGGIVNGY
                     KLSLGTNYPPTNIVYNEQLSLPQYNPGTLYYGSTYYWQVIPYNNEGGDAINCPIWQFT
                     TKQAPPMLSGTVTWAYNNLPQMNVPVKLYDASGNTELQSCLTNELGEYFFEVSYQTYH
                     LKVMAQDIPVNETNVRINAIDNVISITEDTIVDLPLEVYIPPLNTSNCTFVGIYQGHE
                     YWRSNWSAYWPDMNNEFNTRGGHHICISSEGESEFINQYIRQVTGNFHLGYVYNNGTW
                     SWIDGSPVGYTNWAPGQPDGNSEASRIYYESNSGNVYWDDVYYWEYISGIMECNYLTQ
                     PYIPLPVSSFTVQQLRDFSLSLSWVNPTQVLCGLDIADFSIIITRDGEIVQTFTNCLG
                     GQTMNWIDYQLTGQHTYGIYAVNEYGQSESIFQKVQFGNQVQGIALLNNTSNHSGIKV
                     KFIADPATPAAVTDSTYTNSNGFYSINLVIGRYNVIYSKAGYLDASYYNYLHNQPTTL
                     PEQTLEYVGQIMNLSGPLSGTITNEYTYIITGQISVDNGNSLTLQPGVKFYFKNGIGF
                     DVNGSLTAIGTVQSPIVFSSIGSEVRGDMWLRNANIQMEHCEFTKGNYGVRINAPVHI
                     SYSKFHQNNTAVYFDYNSGSNCLIDYCEVYNQNENCFYSYSNDPVKIEHCEIHSNQLT
                     GYILYISYFYKSAAFNNNYVHHNSCYSAFYLENYCVQFILTHNIIEYNTASDRIIYVS
                     NGYRYTHIDISDNIIRYNMGSDLIYISYGSGEFKHNQIYNNSGSVQFSTSEEQYNNYG
                     TISIENNTIVGNSGTGLDITYKYTIKNNIVACNGGSELVYNSNHPALQYNLFYDTSGT
                     IFQNPGGLLFLLDLQAQNANGTPCDPYSNISLDPQFFDSANNNYNLMPTSPCINAGDP
                     NSPFDPDGTIADLGALYYDLIASSHSQIAIPMSMYNFQGTPVGETFIWNCPVRNTGTE
                     DLIISSIELSNPVFFLAVPDKSTTPFSLTVAPNATGYIPIGFTPLAVTDYADTLRINS
                     NALMNPQVSIRIFGSGVLASSVILALPQNNTVNVLSDFQLPLSISDTTPYNIISYNMS
                     VTYDPALLEFVALSTEGTLCADWYVAVNPQGNGVLYIGSSGVNPLTGSGVLFKLNFHT
                     LSGIPDNTETYINIPQIIFNEGGLTVQGCFAPVVIRNIIYGDVDDNQLIQAYDAALTL
                     QYSVMMDPLPNIDPRPWVEWRKLRADVSGDGNIYAYDASLILQRVVGLISSFPVEDQT
                     RVTPVATVNVTYYNGELVLTSPDFKVLYSLNLSLELPQNAETGQPVFSEPFSSALWNQ
                     FTENNIWNFAFACITPSEGNGTICTIPISLVNSTTLALNLVINETPTTFTLECQPTGV
                     QDLVAGTNFLAQNRPNPFNPSTTLYFGLKENAQVELSIYNLRGQKVITLVNKELNAGN
                     HSVVWEGKDATGNYVSSGIYFSRLKIGNSWSKTQKMMLLK"
     gene            complement(15563..16930)
                     /locus_tag="PLG69_02405"
     CDS             complement(15563..16930)
                     /locus_tag="PLG69_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42393.1"
                     /translation="MEFLNRHTEMSILKEELAKKESRLVMLYGRRRVGKSALLQNLDI
                     KDRIYYLADINAAEVQRAFFAAELTKFIPGFNSVQYPDWFTFFQQANRQLTKKITIII
                     DEFPYLVKSSPELPSVLQKIIDLQQNSMFHIILCGSSQQMMQGAVLEKGSPLFGRAQR
                     IIKLQPLECYWLQQALNVTPVQAIEEYSVWGGIPRYWEIRNEFQSMWEAIREAILDPY
                     GILYEEPFHLFMDDLSGAVQPISIASLIGMGCNKLSEIAGRLNKPATNLTRAIQVLID
                     TGYIKRDICWGENYKNSKKTLYRLDDPLTRFFFTFVVPNKSKLNSRQIAIVEKEIKNR
                     WQVYVSETWEELCRQRIVKLTINNTSFFPAQRWWQQTKTGSQTEIDIIAESVDKKDLL
                     VGEAKWQKKIKPETILRELEQKITALPLKKEYQHIYRLVMLPELEFAVQGDIVFAGAD
                     FVCGK"
     gene            17365..17646
                     /locus_tag="PLG69_02410"
     CDS             17365..17646
                     /locus_tag="PLG69_02410"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPI42394.1"
                     /translation="MKNDPKYQYMSTIMKAMAHPTRLFILDLLAEKEYCVCELQKQIG
                     TDISTVSRHLAVLKNAKLICSHKVNNQVWYNLLCPCVLNFYNCLSQIKY"
     gene            17667..18083
                     /locus_tag="PLG69_02415"
     CDS             17667..18083
                     /locus_tag="PLG69_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPI42395.1"
                     /translation="MTNENTCSCSCNCNTGEGNSKCATNLATNKVYACGGASNVGVIS
                     FELAKTLHLKNQYKMGCTSGVAAGEFEVQNTIQQEGNQDLVIDGCPIACVQKIFEQKG
                     IKGFKHIIVTEFGIDKEATFDFEPAIIIQMIDKIMY"
     gene            18476..20992
                     /locus_tag="PLG69_02420"
     CDS             18476..20992
                     /locus_tag="PLG69_02420"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPI42396.1"
                     /translation="MKEDYIQKNDYRSIFFTDKAEECESELKSLFLNHLEYSLIKDIT
                     TVKPWDIYYALALSLRDLLIERWLRTQYEYRKQDVKKVYYLSLEFLLGRMLTNSLINL
                     DVYNEVYDMLKEMGISLEDIIELEPDMGLGNGGLGRLAACFMDSLATQAYPAYGYGIR
                     YDYGIFKQQIVQGYQVEEPDNWRKNGCPWEINRPELTYRVRFGGKVISETFPDGRIEH
                     HWIDTEDVLAVAWDIPVPGYQVDNVNNLRLWQATSTAEFDLEYFNNGDYVKAVEKKTI
                     SENISKVLYPNDNVHLGRMLRLQQEYFFVSATLQDIFAHWKRYHNSFNNFADKIAIQL
                     NDTHPALAIPEMLRILIDEERMSFENAWEITKRCFSYTNHTILPEALEKWGIALFEEL
                     LPRHLMLIYQINNVVMEEVVRLYPGDLAKMRNLSIIEETNGKAIRMAQLAIHSSHTVN
                     GVAKLHTKILCERIFPDLAELYPGKFQNKTNGITPRLWLHTCNPQLASLISEYIGSGW
                     ITNLEEIRGIEKYINDSDFRTSFAEVKDINKSHLSRYIYRETGIRVQVNSLFDAQIKR
                     LHEYKRQLLNVMGTIARYFRIKDNPNGNFVPRTVIFAGKAAPGYFLAKRLIKLINNIG
                     EVVNKDPDIKDRLKVVFLPNYCVSLAERIIPAADLSIQISTAGYEASGTGNMKFALNG
                     ALTLGTLDGANIEMAEEIGAENMFIFGLTAEEVKELNQSGYNPRSYYESDPELKRVVD
                     SLIDGTFEEGEKDLFLPIWKALMEDGDSYLNMADFRAFVETSNKVDEFYLNREEWITK
                     AILNVARVGKFSSDRAIKEYADDIWGIKPLPLELNNGNNS"
     gene            20998..22611
                     /locus_tag="PLG69_02425"
     CDS             20998..22611
                     /locus_tag="PLG69_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPI42397.1"
                     /translation="MAWIRNFILFAFWGFLCLALRAEIVINEVCYDPVGSDEGWEWIE
                     LYNNGNTSVQLEGAKILSGGSSFTLQYTFPFFELRPHRYLLIGGASILNAQLYYNFSF
                     QNGGSETDGIRYVSPEGTYTDTVLYDSPNTNQLFDDHNLAGTHFAPDVPAGYSLARIY
                     DGYDTNNCETDFIAEAKPTPGLPNRIHCDYALGTYSVLRENNYVELDLWLKNLSFLVP
                     ILSAELTVTQNNELLAQQAIAPIPALDSLLVNLAFFCASSPLTVKLSLEDDPDSTNNV
                     LLVPLTPEMEEGIYINEFLANPESGNQEWIEIYGAQISQGNYYIADSSTSQIRFSLPA
                     TSGYYVICREPDALIWRYPECPAASIILAESWTYLNNAGDCLVLKNETGTLDSLNYSG
                     EEIIKGVSRERVTADSTVFWQNCYSEIGGTPGLPNSCMPQTELPDLGKVVLSGSPCNA
                     KAGEKISLAYNFPSATNRITCNIYDLNGRKIFTVADYALVNAAGVLYWNGCKQDGNFA
                     PRGLYIVLWESQNAGGGKIMRKQFTAVLK"
     gene            complement(22762..23262)
                     /locus_tag="PLG69_02430"
     CDS             complement(22762..23262)
                     /locus_tag="PLG69_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPI42398.1"
                     /translation="MIELRDGDITLFEGEAIVNAANSSLLGGGGVDGAIHRAAGKGLL
                     EECRTLGGCKTGEAKITKGYNLKAQYVIHTVGPVWQGGNFREAELLASCYKKSLELAV
                     EKGIKSIAFPNISTGVYRFPKEEAGKIAVETVREFLPQHPEIDVYFYCFDRENYDIYC
                     RLLQEN"
     gene            24120..>24672
                     /locus_tag="PLG69_02435"
     CDS             24120..>24672
                     /locus_tag="PLG69_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI42399.1"
                     /translation="MQAIRLKNIHFTYPDQYQEILSGITLDICDGQKIALIGKNGCGK
                     STLLKIIRGELQPTSGSISYPIAVPKIGYLPQDLMISNQLTVRDFLLQGDINSYNIIR
                     EINTLSAEKNLCVEDGLHLAELWQNYEHGNVLFWEQNVDEVIRKMDLNALANQSCNTL
                     SAGETTRVQLASLLLQNPDILILD"
BASE COUNT         7642 a         4669 c         4714 g         7547 t
ORIGIN      
        1 ctcccaaatc cttccaggga agaacttcag ggtctttttc cgcaaaaatc ctgaactttt
       61 tgccatttac cactaggtaa ttctcttcgc tggaaacatt gccaggatat atcttatgca
      121 cactgtcata tttaaataga tgtgccagtg ttttagcatc cgtaagatca tttatagcta
      181 caacattcag ctcaggatgg ctatccaaaa ttacccgtaa aaccagacgt ccaatgcgtc
      241 cgaaaccgtt gattgctaca tttgtcataa tatttctcct tataagtatt tatatatcta
      301 ttagatagat tgctaccgga actgctggaa aatgcctttt taacctttca atatcgttaa
      361 tcagttgttc tccataagaa acacctgtgg acattgactt gtgtaattgg taacagggaa
      421 ctatttctat tcccacatcc gctttgtttt cattgaaaaa gtattgaaat ttggcaagat
      481 cataaaacat aaaagggtat tttcccaatt gcacttccac aagaactttc cctaaacaga
      541 agtcaatttg tttataacaa ccggttattt tcggttcgta atttggtaat tctatactaa
      601 aagtatctct cagttcttta taacctaata attgaaattt gtctttgaat tgtttattta
      661 tatctttagg agaaaaaagc gatttgccac tcattgtttt ttctttacta atcttggttt
      721 tgtgaggttt aatatccttt ataatgttta atacttcttc cataatttta ggatatctta
      781 ctaataatat ttccaaaccg ccaagatgtg agtattcata tacaatattc acttttcatt
      841 actccaaacc agatcaagtt ctgactgttc ttcttttatt ttggttaaat ttaccgtaat
      901 aggtttgaat gaaataggat gttcgggatc aaagatcggc ctattcatag ggcgtatttt
      961 caattcaccg gaaatggcac tattaattct ttgcagggca atttcataat attcatcaag
     1021 aagttccgct ccacagcttt tccggttatg aataagcgaa gcaatttgcg ttgttcctgt
     1081 tcccataaaa gggtctaaag tccagtcatt ttcattggtc atagataaaa ttaggcgttc
     1141 taccaattct acaggaaatt gggcaggatg aattgttttt tcaatatgat tagctttcac
     1201 attgggaata ttccatacat cggaaggatt tttgcctaaa ggattgcaag ataattctcc
     1261 ctttttgtct cccttaaaat atttcttatt aggatacttc tggggaattc ggactgggtc
     1321 taaattgaag atatattcat cacttttggt aaaccagaga attacttcat agcgtcctga
     1381 aaaccttttg gaagaatgca aaccatgccc gaaatgccag attattctat ttcttagttt
     1441 taagcccgat tcagcaaaaa caggataaag taaaatatcc agagggataa tttctccgtt
     1501 ttcaatataa tttcctacct gccaacagat acttccactt ttttttagaa ctctacagca
     1561 ttctgtaata acctttttct gccagtcaat atattcatct aagggttggc gtttttcata
     1621 ttttttgcct atattatagg gaggtgaagt aactactaac tgaatagcgg catccggtat
     1681 ggaatcaagt agttccaaac aatcaccctg aaatagacag agaatggaat cagagttcca
     1741 tttattgctt attttaacca tatcagctat aacaaaatac tgttattagc gctaccggac
     1801 attttgcaga attccataac cgtattctgc attttctctt cccctttgat taaccattta
     1861 cgagggtcaa acttactttt attaggtata taggcagcag ggtcaacatc aggagggcaa
     1921 acaatggcat atttttcctt ttcccaatat tcttgaatag cagaagccat atccatctgg
     1981 taatgagtat ctttattgat tttcaccaca ccattggcaa tagctgtttg ttgttgccaa
     2041 tcagttaatc cggaagctcc atgtaaaacc agtcctcttt ccgctccatt tttaatcagc
     2101 aggtcatcaa tttccttaat taaatctaaa tgcagaacta cattttcacc tttagtaacg
     2161 ccatgatttg tgcctacgga agcagcaaaa aggtctacat tggtctttat tacaaattcc
     2221 aatgcctctt cgggtttggt atagagctca tcttcggaaa caatttcatc ttcggtaccc
     2281 ttgatatgac cgatttcacc ttctactaaa atgttatatt tatgtgctaa atccacaacc
     2341 tctttagtaa tccgaatatt atcctccagt ttttctgccg aggcatcaat cattacagag
     2401 gaaacgaggt cgttttcaat acagaactta ataaaatcaa aatagttagg tgtggcatgg
     2461 tcaatatgca atccaattcc acatttggga aactgagcag aaaagatttt taccattgcg
     2521 gaaatcattt ttgcccctat atgcaaatcc tgagaagtgc ctgctgcata tttacaggca
     2581 ccagtactca tttgcattaa gccatcagaa ttgacagccg cgtaaccttg aatgagagct
     2641 cttacctgtg tgtcaaaaac aacattggat gcgatgatac caaaacgctg ttttttagct
     2701 ttgaggaaga cctctttaag ttgtgttccg gttaaaaaca ttttatacct ccacttgtgt
     2761 ttagttctta taagataaac tatccaaatg ttgatataaa gtcaagggaa atctgcagaa
     2821 gaaagaaggt ggagaaaaaa tctccacctc tttattttat taagaatagg gtttcttgtt
     2881 tagttagttg ctataacccg atagaatttt ttggaaacag ccgcgccact atagaataaa
     2941 ttgggagttg tagttacggt agaccaggtg ccataaggat catctgcgga ttcaattctg
     3001 tagttagtag ctccaggaac ctgattccag ctaaggttta tcattccagc tacttgagaa
     3061 atattaacaa tcggggcttc caagattcca gcagcgggag ttatatctgc tagaaaacgc
     3121 ggactgaatt gcatcgtgtc attatattga cctcccagac agacgatatc cacagggtct
     3181 gttggaatag cggttccggc ataatcggca ttggggaaag ttctcataac acccgttccg
     3241 gaagcatcac tggcattaat attttctgct gtagcagcaa aaacgccagt agcattggga
     3301 gttaaagtta cactatatat cttaagcagt tttgcctgat cggcagatgt gatagttgcc
     3361 aaagttctcg ttacaggaac aattgtatta ttatgagagg tggcagctcc tggatcagca
     3421 ataggaacaa attgtagtaa tccagaataa gaagataaag ttcctgttat tccggttatg
     3481 ccatcgtaga ggttataagt agtggttatt ttgccgttag gatcatcaat aactatagca
     3541 gctgttgcat cctgaacata tttttgatta cgatttgcct gctggaaagt aagaactgct
     3601 tcacccgtaa ggcgataggc actacctaaa gttccttctc tcagttgtgc tatagtagaa
     3661 acattttccg gaaaatcata aaaagcagta gcaattgcag agggcgaata accggtagca
     3721 taacctttag ctttgatagt ggtatcggaa ctgacaggta ttggagcgct gtaaacagga
     3781 gatgcctctg tgggctcaga accatcagta gtatagtgaa ttacagctcc aggagttgtt
     3841 gtgctgatag ttacatttat tggagtagcg taagcacccg caggaggatt gaaaacaggc
     3901 ataactacca tattgccaaa ggtatgcatt cccagattat cagtataatc ctgggcataa
     3961 acaatccatt cactatcatc ggcattagtt ccagcactga tagcccaatc caaattgccc
     4021 tgagtaattg tgggtttacg gattaaggta tgttcagcag tagcatttgt aactcctgct
     4081 acattccatg ccgttccggg atctgtgcca atagtgccga taacatctat tctggtggag
     4141 tccgttccca cttttttata aagcaaaaga gcatcatcac cgttataata agtaacggta
     4201 gaagttgtat ctgccaatcc caaaatagtg gcatttgcag aagcattggc aattacataa
     4261 acatcaaggt gagcaagagt tcctgttaaa tttaatgtat ataaaggtgt ggcagcgcca
     4321 ttggtataaa gacaaacctt gtaattgctc aagtccactg gtccaccggt gccgttaaaa
     4381 atttcaatag ctttattatt acttgtccct tctatatatt cagagaagaa caaatcactt
     4441 gccattccgg gttgagatgt ggcagttccg ctaacgggaa gttgatattc attagcataa
     4501 gtagtattat ggtaaattat ttcgttgaat gttccagaac ctgtggcatt catccgtaca
     4561 taaacatttc cgctaaaatt attgttcagg gttaaactta ttccccagtt acctgttccc
     4621 gtagaactta attcaaaagg ataggaagta gttatttgaa gagaatcaat tgctcccgga
     4681 gcacttaaag tgtatgattg aactgcagaa ggagttcctg cttcagaaga aaaaggagtt
     4741 aaattggcag taatattcca ggttacaggc gggggaagag ctgctcctgt tactctaaca
     4801 tttacaggtt cggcatctcc actggtatga acaatatagc cctcatattc accgatggcg
     4861 tttgccagca tccgcacata aatttgcgag tgaaaatcgg cgggaacagt tatactggaa
     4921 gcccatcctt ctatttgaga agtagaaacc tgaaagccgt tgggggctgt aacagttatt
     4981 ccacctacga tattagttcc ccataaagta taatctctgg tttcgtctga gggaagattt
     5041 acaatgcttt ccaaaggatc aagctcggtt gtgctggtat gaataactgg agtttccata
     5101 ggagaccctt cccaaagttt agcccagtta ttggttaccc ggatgccatc aattcttgct
     5161 ataggaccat tagaaccttg acgaatagcc actgctccaa ttgctcctat atctgttcct
     5221 gttaaatcag cagccgtaag ttgaggtgca ggttcagttt ctccgataac tggattaacc
     5281 cacatataga cctcatcatt agtagcacct gaaacaatta catatttaac cacaatcaaa
     5341 taggttgtat ttaaactata gctgtaagta gtagctgcag attctgaaat agcacttgnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ataaacaagt ccgctgaaat
     5521 tactggctaa agtaagagtg ttggtaaagg tagtattatc tgtagaaagg gcaaaacctt
     5581 ccggtgcagt aacacttatt ccggcagtta aaaatagtcc ggtaactgta taactttgcg
     5641 cagcagaagg agttcccaat tgtgtaccga aggggtttaa gttagctgta atattaattg
     5701 ttggagtagg atcggtaaca gttccttcca cagcaagatt tacattagtt gctccggtac
     5761 tggaatgcac aatattgccg gaaaaatttc ctgctgcagc tccggttaag cgtacatata
     5821 ctaaaccgtt aaaagtggct ggtaaagata aattgctggt ataggttgtt ccatcagtag
     5881 aaagagcaaa acctgtagga gcagcaaccg tgatatttcc ggttaaattt tgaccgctca
     5941 aagtgtaact ctgagctgtg gaaggagtac cggtataagt agaaaaagga gttaaagtgc
     6001 cactaactac aataaccggt gtgggtgcac cttccgcagt aatgcttaca tcgtcaattc
     6061 ccaagccctg agcattggaa gtggttgaac catttgttac ggaatagttc caagcaagat
     6121 atacagaact tccagctgca agggtatagg ataaagtagc agatactgga actgttgccc
     6181 ctggagctga atcatagcca ctatttgaag catctgaacc tgcaaatgaa gttaaaaatg
     6241 aacttcctgc agaagtccag gttgctccat ctgcggaata atacatctga atggcaaaac
     6301 cggctgcatt agttcccatc cggtattttt ccacattgta gcttatggaa aaagaattga
     6361 tattagtgct gccgttattg gttaactgaa catagacatt tccacttttg gtagcagaac
     6421 cggaagatat aaaaccaatt gctctatcgg tagcagtagc tgctggacct gccccataat
     6481 tgtaaatgcc attttgagca gttgaactca tattattccc tgctattagt tcagtgacag
     6541 taccagcagc agaataagtt cccacacttc taacggttgt atttttatca gctttccaac
     6601 cggtaggtat ggttgcagca gctgaagttc ccaaaacatc aaagttttgg gttacattct
     6661 ggccactttg aatatttacc tgtgcgaata accctcctat aaaggaaagg gttagtagaa
     6721 tcaaaaacaa tgactttctc atatttcctc cttgagaaaa cttagttcct aacttataat
     6781 cagatatatt aattacgcat aaagtgttta atatttgaag aagggtatgg cgtcaagtaa
     6841 aataatatgt tcttttattt ttgttcagat gctgctgatt attattaaca ccgagggcac
     6901 cgagatttat ttacaaagag agaaagagga aaggagattt attttacaaa gagaaaaaga
     6961 ggaaaagaga ttttttaaac accgagaaca cagaaagcac cgatattact ttataattta
     7021 tgaactatct ttattcacag gtcgtcaaga gtcctcattg ctaaactaat ttgaagaaag
     7081 cgatatagtt atacaagcaa tgacgactcc tgacttaatt ctttctccat actccattca
     7141 ccgttcactt ttaacttttc accgtttaca atctcaccat ctcgttatgt caccacctcg
     7201 ctatttttta acttattcac ttattttccc gttcactcca cacactttga aaaatctatg
     7261 ttccaattgt gaattatcaa cccacagtgt ttcctggaaa ttagcagggc ttattaaact
     7321 ccaatcagta agattagagc tggcataaac attatagctt aggcaattaa aggtaggaag
     7381 ccagctgatt gtgggtactg tttctgtttt ggtaattaaa acttcaggcg tttgaggagc
     7441 aagaaaattg ctcatatcta tatcatctat aaaaatgccc gaatcacaaa ctaattttac
     7501 tgaaacactt aaatctatat aatcagaaaa agaaatccgg tgataagtcc agttattatc
     7561 taaagggata ttggagtgat agaggtagtt ttgttctatc gcataaccgg tactgttcaa
     7621 ataaatagct attgagcttg ccgaattagc tttagcccag aaaccgaaac cttttccgtt
     7681 accaccttgc atccaggagc tgattagata catttcaggt gaaatagttg tccaggaaga
     7741 tgagccatta tgtttttccg cagatgagag ttgccaatta ttatctatat aaccggaatt
     7801 ggttaagctt tcgcagggct gtatttcttc tccgcaattg gcttggagat aaagaattgt
     7861 attacctcca ctggaggtta tttgtaaggg atagctacct cctcctgttg accaagggtg
     7921 ataactaact tcaatagtat tgctatttcc actgaaactg ttcactccga aaaagccctc
     7981 aggatagatt aaatttccac tcaagatacc tgttccgcta ttgcttatac tgaaatttgc
     8041 attagctgtt tgtcccggtt ttaaggtcgg aaaaaccaaa acagaagtgt tcaaagtgat
     8101 ttgaggaata tactgttcca attgaatatt atctataaag aggtaattac cgttattgga
     8161 aacccgttta aaggcaagat agatactctg gctggcaaat tgggttaagt ccacagtgaa
     8221 agatatataa gaggtagtaa cggcaaaatt atccaaactt aagagacaat gggtaaagtt
     8281 ttgaggtgtt gaaccagtag tagaaagcat tatctccaaa ttctcaccca caatgtcata
     8341 agttgaattg ctggaataat ccttaatata gaaacttaag gcagaaccgc aattgggatt
     8401 tattactgct gggctaatat accaataagt tccaggtgct cttcctactg ttatacactt
     8461 gcttccagaa attccgttgg tagtatttat tcgccaatta ctacctgcag aaccactata
     8521 aaaagtagtc cagccaccgg aagttacgct tccatcataa aaatctttca ggtaaggata
     8581 ctggacaata gcattggcat catgagtagt aaaactccaa attggacagg aacttggctc
     8641 tccataagta ttgtaaggaa ttatctgcca ataataaaca gtattatatt gtaatggcga
     8701 agaaggttgc caagtagtaa ccagtcctaa atctgttcca tttacaatat tggaaggagg
     8761 tgtattagtt ccaaagtaaa gtcggtagcc aagaggtgga tcaccaagtc cactttgcca
     8821 acttaaaaca ggattgagaa taacatttag ggcattgggc aaaggactga ggcaaacagc
     8881 tgaaccgggt agtggaatta gatgccctgc cagttcttgc cagggagaaa tgaaatcacc
     8941 aatccttaaa cgaattctgg caaatagagc tgttccataa accaagttaa gcagctgttg
     9001 gctggtgtaa ttttcaatcc agccactagc ataatattct gctatctccc aattatcatc
     9061 acttccggct tggaattcac attcaaaggg cgttgcactg ctgttaataa cattccagat
     9121 aaagagcggg ttgttattat ggatagccat aatatccgat tccccctcta tatgcaaatt
     9181 ccagagatta taaacatcca atcgtccatc cacaacactt actgcaggtt gtgtattgaa
     9241 aagaacagtt tccagctgga gatggctaaa agttatagca gtaggcaaaa cctcaatgtc
     9301 tataaagatt agtgttccat ccgcagttat gggcatagtt cccattccag caacagaaat
     9361 taaaccgctg gaagttctat tgttaatttc ccatccctca ctgctactac cgctattatt
     9421 tacgctgata acattaataa cagcgggatc aaacctgaac ttgaactgaa aactgataca
     9481 attcatacca gctagtcctg tagcaataat tggaagcgaa aaagaacttc ctgtttgcag
     9541 gagatagttc cctacagaga gggaagcact aataggttga ttttctatat agatacttcc
     9601 cccatataag ttcataagcg atgttgtatt cattacagca gaaacaaaat tcacttctgc
     9661 caaaccaggg ttttgagcac tgaatttcaa gttcaaaagg ttgccctgtc cgtataaagg
     9721 attagtgaaa atagctccaa tgtgtaaaat tccatttact tcattaacgc taatcatacc
     9781 attggcagaa agagtattgg tcttcactat ttccagaaaa gtcaattttt gggtatcata
     9841 ggcgatattc agttcgtagc tgattactcc gtatccgctt aaagaagatg tctgaaccgg
     9901 caaagtggtc tgttcaccca agatgatatt ttgttctcca atatccagct gaatatcttc
     9961 aatatccgcc agtaaaaaga ggcaactaca agccaggagc ataaagatta ttagtttttt
    10021 cataataaca gctcctaaag cttatttcag taacatcatc ttttgagttt tactccagga
    10081 attgcctatt ttcaaacggc tgaaatagat accactgctt acataatttc ccgtagcatc
    10141 ttttccctcc caaactacac tatgattacc ggcatttagt tctttattca ccagagtgat
    10201 caccttttga cctcttaggt tataaatact aagctctacc tgggcatttt cttttaaccc
    10261 aaaatatagc gttgtagaag gattgaaagg attaggacga ttctgtgcca ggaaattcgt
    10321 tcccgcaact aaatcctgaa ctccagtggg ctgacattcc aaagtaaaag tagtgggggt
    10381 ttcatttatt accagattca atgccaaagt agtggaatta accaaagaaa taggtatagt
    10441 gcaaatagtg ccgttgcctt cagagggagt tatgcaagca aaagcaaaat tccagatatt
    10501 gttttccgtg aactgattcc acaaagcgga agagaaaggt tcagagaaaa caggttgccc
    10561 ggtttctgca ttttgcggca gttccaaaga gagattcaaa ctgtaaagga ctttaaaatc
    10621 aggagaagta agaaccaact ctccattata ataagtaaca tttacagttg ccacaggggt
    10681 aacccgcgtt tgatcttcca ctggaaaact gctaattaaa cccactaccc tttgtaaaat
    10741 aagcgaagca tcataagcat aaatattgcc atcacctgaa acatccgctc tcagttttcg
    10801 ccattccacc caaggacgtg gatcaatatt aggtagggga tccatcatta ctgaatactg
    10861 caaagttaaa gcagcatcgt atgcctgaat cagttgatta tcatccacat cgccataaat
    10921 aatattgcga ataacaacag gagcaaaaca accctgaacg gttaaaccgc cttcattgaa
    10981 aataatctgc ggaatattaa tataggtctc tgtgttatcc ggaattccgg aaagagtatg
    11041 gaaattcaat ttaaaaagaa ctccacttcc cgttaacgga ttaactccgc tggaaccaat
    11101 atagaggaca ccattgcctt ggggattaac agctacatac caatccgcac aaagagtccc
    11161 ttcggtgctg agagcaacaa attccaaaag tgcgggatca taagttacgc tcatattata
    11221 ggagataata ttataagggg tggtatccga aatagaaaga ggcaattgaa aatcggagag
    11281 cacattcact gtattatttt gcggcaaagc cagaattaca ctgctggcaa gaactcccga
    11341 cccaaaaatc cggatagaga cctgtggatt catcaaggca ttgctattaa tgcgtaatgt
    11401 atcggcataa tcggttacgg caagtggagt aaaaccgata ggaatataac cggtagcatt
    11461 aggagcaaca gttaaactaa aaggagttgt actcttatca ggaactgcta aaaagaaaac
    11521 cggattgctc agttctatgg aagaaataat caggtcttcc gttcctgtat ttctcaccgg
    11581 acaattccaa ataaaagttt ctcccactgg cgttccctgg aagttataca tactcattgg
    11641 aatggcaatt tgagaatgcg acgaggcaat aagatcataa tataaagcac ccaaatcagc
    11701 tatcgtgcca tcagggtcaa aaggactatt aggatcaccg gcattaatac aagggctggt
    11761 aggcattaga ttgtagttgt tattagcaga atcaaagaat tgcggatcca gcgaaatatt
    11821 actgtatgga tcacaaggag tgccattggc attctgtgct tgtaaatcaa gcaggaaaag
    11881 caagccaccg ggattttgaa atatcgttcc actggtatca taaaagagat tatattgcaa
    11941 agcaggatga ttggagttat acaccaattc cgaaccccca ttgcaggcaa caatattatt
    12001 cttgatggta tatttatatg taatatccaa accagttccg ctattaccca caatagtatt
    12061 gttttcaatg gatatggtac cataattatt atactgctct tcagatgtgc tgaattgtac
    12121 ggaaccacta ttgttataaa tctgattgtg tttaaattcc ccagagccat aactaatata
    12181 tatcagatct gaacccatat tatagcggat aatattatcg gatatgtcaa tatgtgtata
    12241 acgataacca ttactaacat agataattct atcagaggca gtattatatt ctattatgtt
    12301 atgggttaaa atgaattgta cacaataatt ttcaagataa aaagcactgt aacagctgtt
    12361 atgatggaca tagttattat taaatgctgc cgatttataa aagtaagaaa tatataggat
    12421 ataacctgtc agttgattac tgtgaatttc gcaatgctca attttaacag gatcattgct
    12481 ataagaataa aaacaatttt cgttctggtt atagacctca cagtagtcaa tcaagcaatt
    12541 tgatcctgaa ttataatcaa aatatacagc tgtattattc tgatgaaatt tgctgtagga
    12601 aatatgcacc ggggcattta ttcttactcc ataattcccc ttggtaaatt cacagtgttc
    12661 catttgaata ttggcattcc tcaaccacat atctcccctc acttcagagc caatagagga
    12721 aaaaacaatg gggctttgca ctgtaccaat agcagttaaa ctgccattca catcaaagcc
    12781 gataccgttt ttaaagtaga atttcactcc cggttgcaaa gttaaagaat ttccattgtc
    12841 cacagagatc tgcccggtaa taatataagt gtattcattg gttatcgtgc cgcttaaagg
    12901 tccgcttaaa ttcattattt gtcctacata ttccaaggtc tgttcgggta aagtagtggg
    12961 ctggttatgc aggtaattat aataggaagc atctaaataa cctgctttgg aataaatgac
    13021 attataacgc ccaataacca gattaatact gtaaaagcca ttggagtttg tatatgtgct
    13081 atctgtcacc gcagcaggag tagcaggatc ggcaataaat ttcactttga tcccggaatg
    13141 attactggta ttatttaata gagcaatacc ctgcacctgg ttgccaaatt gcaccttttg
    13201 gaaaatactt tcactttgac cgtattcatt tacggcataa attccataag tatgctgacc
    13261 ggtaagttga taatcaatcc aattcattgt ttgtccaccc aggcagttgg taaaggtctg
    13321 cacaatttct ccatcccggg taattataat agagaaatcg gctatatcca atccacagag
    13381 tacttgggta ggatttaccc aacttaggct aagggaaaaa tccctcaatt gttggacagt
    13441 gaaagaacta accggtaaag gaatataagg ttgagttaaa taattgcatt ccattattcc
    13501 actaatatat tcccaataat aaacatcatc ccaatagaca ttaccgctat tactttcata
    13561 ataaatacgg ctggcttctg aattgccatc tggctgaccg ggtgcccaat ttgtatagcc
    13621 cacaggagaa ccatctatcc aagaccaagt gccattatta tatacatagc ccaaatgaaa
    13681 atttcctgtt acctgcctta tatattgatt gataaattca ctttcccctt cactgctgat
    13741 acagatatga tgtccgcctc tggtgttaaa ttcattattc atatcaggcc aatatgcact
    13801 ccaattacta cgccaatatt catgaccttg gtaaatacct acaaaagtgc agttggaagt
    13861 attaagagga gggatataga cctctaaagg gaggtcaact atggtatctt cggttataga
    13921 aattacatta tcaatagcgt ttattctcac attggtttca ttaaccggga tatcttgtgc
    13981 catcactttc agatgatagg tctggtaact aacttcaaag aaatattctc ccagttcgtt
    14041 agtaaggcag gattgcagtt ccgtgttgcc actggcatca taaagtttta cgggaacatt
    14101 catctggggc aagttattat aagcccaggt tacagttccg ctaagcattg gaggtgcttg
    14161 tttagttgta aattgccaaa tcggacaatt tatggcatca ccaccttcat tattgtaagg
    14221 tatcacttgc caataataag ttgacccata atacaatgtc ccgggattgt attgcggcaa
    14281 gcttaactgt tcattgtata ctatatttgt aggaggataa tttgtgccca aagataattt
    14341 atagccatta acaattccac ctccgctatt ccagctaagc attgtattaa tttccacatt
    14401 ttccgctaaa tcagccggaa aaggatttac tgctggaaag ggtggctcaa ttaaagaagg
    14461 agggttccag gtaaaagtta atccattggc tggaaaaacc gtttcactta aaatacaata
    14521 actgttattc tctgttccag ctgtagtggc agaccagtca gtagcagtag ctctattaca
    14581 aaaatcggta ttgctattcc ctcttaagcc cacctggata gattggctag tagaagaagt
    14641 tacagcagta caaggtccgt aaacaaaaac aacctgatta ctgccttcat aaagttgtat
    14701 ctgaaaactt aaatcgtcat tgagggtggc tgttggtctc cttctatagt gcatccattg
    14761 cacaataaat atccggttgg agtccgttcc aatagttttt gtcatcagcg agccgttatc
    14821 acgacttatg agatcacgat tcattaccgc aattacatta tttgtagctg tctcactact
    14881 gagagctaca ttgctggtgg taacattatc gcccatagcc aaaaaaccat tggaagcaat
    14941 gcttattgta gtataggaat tgccattgta actaaaggta aatcctatag gaatagaaag
    15001 aaaacattca ttgtcattga ggttggagcc cagaacggtt ccattcgtta tttctgaaaa
    15061 acttcccaag gaagagctaa agctatattc cgcaacggaa gccatcagaa agccacacaa
    15121 acaaagtaaa aacaaggtaa agctaaactt gaaagtaagt tctttcattt ttgttattct
    15181 cctctattta atgattgttg attcccttat tattgttgct tacacactat aaaatattcc
    15241 aatgttgcaa aacaaactga ttaaacatac ttacattaaa aaatcaacct gaattttctg
    15301 tcaactaaat tttatcaacg ggtgattttt taccccgaaa gcaccgaaaa caaagagatt
    15361 ttttacaaag agaaaaagag gaaaagagat ttattttaca aagagagaag gaggaaaaga
    15421 gattttttac aaagagaaaa ggaggaaagg agattatatt tatacaccga aaacaccgag
    15481 attttatgat aattttaatt tatctgtgtt gcttattcag gattaacctt taacctttca
    15541 ctattcaccg ttcaccctct cactacttac cacacacaaa atctgctcct gcaaaaacaa
    15601 tatctccctg tactgcaaat tcaagttccg gaagcattac taaacggtag atatgttgat
    15661 attctttttt aagaggtaga gcagtaattt tctgttccag ttcccttaat atggtttccg
    15721 gttttatttt tttctgccat tttgcttcac caacaagcaa atccttttta tctacagatt
    15781 cagcaatgat atctatttcc gtttggcttc ctgtttttgt ttgttgccac caacgctgtg
    15841 ccggaaagaa acttgtgtta ttaattgtaa gttttactat gcgttgtcta catagttctt
    15901 cccatgtctc agaaacataa acctgccaac ggtttttaat ttccttttcc acgattgcta
    15961 tttgtctgct gttcaatttg gacttgttgg gaactacaaa agtaaaaaag aatcgggtta
    16021 gaggatcatc cagacggtat aaggtctttt tactgttctt ataattctca ccccagcaga
    16081 tatccctttt gatgtagccg gtatcaatca gaacttgtat agctctggtt agattagtag
    16141 ctggtttatt caatctgcca gctatttcag aaagcttatt gcagcccatt ccgattaaag
    16201 aagcaatact tataggttgc acagccccac tgagatcatc cataaataaa tggaagggtt
    16261 cttcatagag tatgccgtaa ggatcaagaa ttgcctctct gattgcttcc cacatacttt
    16321 gaaattcatt tcttatttcc caatagcgtg gaattcctcc ccaaacagaa tattcttcta
    16381 ttgcctgaac aggagtaaca tttaaagctt gttgcaacca gtaacactcc aagggttgga
    16441 gtttgataat cctctgagct ctgccaaaaa gaggagaacc tttttccaga accgcacctt
    16501 gcatcatctg ttgcgaactg ccacaaagaa ttatatgaaa catagaattc tgttggaggt
    16561 caattatctt ttgcagaaca ctgggtaatt cgggagaact tttcaccaga taaggaaatt
    16621 catctattat aatggttatt ttctttgtca attgccggtt tgcctgctgg aaaaaagtga
    16681 accaatccgg atattgaaca gaattaaaac cgggaataaa ctttgtcagt tcagcagcaa
    16741 aaaaagctcg ctgaacctct gcagcattga tatctgccag gtaataaatc ctgtccttta
    16801 tatctaagtt ctgtaaaaga gcactttttc cgacccgacg acgtccataa agcataacta
    16861 accggctttc ctttttcgcc aactcttcct taagtataga catttcagtg tgtctgttta
    16921 agaattccat aataaacctc gttattttgt attcgggact atactttttt aatcattatg
    16981 tttgtcaatc attatgtttg atgatcataa ttataagctg aggttatgaa attacattcc
    17041 gctggttttt ttcaccgagg gaacgagatt tttttacaaa gagagaaaga ggaagagaga
    17101 ttatttttat atatcaagaa cacaggaaca ccgagatttt tttacaaaga gagaaagagg
    17161 gtttggaaaa ggtaaaagta aatatcagat aaaaatattt cctttttttg aatatttctt
    17221 ccgcttagtc ataaattatc acagttatgt cctcatccct tattgttcaa cggtcttata
    17281 atgtatctta tattatcctt accatcgttt tccttgacaa atttgcctat tttgccaaat
    17341 agaaaattga aggaaaatga atatatgaaa aatgatccta agtatcaata tatgagcaca
    17401 ataatgaaag cgatggcaca tccgacacga ttatttattc tggatttact tgctgaaaag
    17461 gaatattgcg tatgtgaatt gcagaaacag atcggaacgg atatctcaac cgtttccagg
    17521 catttagctg ttctaaaaaa tgctaaacta atatgtagtc acaaagtgaa taatcaggtc
    17581 tggtataatc tgctatgtcc ctgtgtgctg aacttttata attgtttgtc ccaaattaaa
    17641 tattagatcc aaataagagg tgaaaaatga caaatgaaaa tacttgtagc tgttcctgta
    17701 attgtaatac aggagaagga aatagcaagt gtgcaactaa tttagcaaca aacaaagttt
    17761 acgcatgtgg tggagccagt aatgtaggtg tcatttcttt tgagcttgca aagacattgc
    17821 atctgaagaa tcaatataaa atgggttgta catccggagt tgcagctggt gaatttgaag
    17881 tacaaaatac aattcaacag gaaggtaatc aggatttagt gattgacggg tgtccgattg
    17941 cttgtgtgca aaaaatcttt gagcaaaagg gaataaaggg ttttaagcat ataattgtga
    18001 cagaatttgg catagataaa gaagcaacat ttgattttga acctgctata attattcaaa
    18061 tgatagataa aataatgtat taggactgaa gagtaaaata aggcaaaatt ttagttaaag
    18121 tcatcatatt ttaggtgtca aactcaattt ttacacttca tttccaattg ggtaatggaa
    18181 gaggtattct gcttatttca ttattttgct tctgatttaa tttttcgtgt gccttgtcgg
    18241 cttttgagaa tggtagtata aacataaaca agtgatatca actgtatgaa agggacaaaa
    18301 taatagctat aagacctaaa atctgtatta taataaatta tatttgcctt cttcattttt
    18361 cttggtgttt tggttgtttt caatgcccta atgtaaattc ctcatttttt acttgacaac
    18421 aattcccttt tccccagttg gaaaagtgtt cataagaaca gagaaggtgc aatgaatgaa
    18481 agaagattat atacaaaaga atgactatcg ctccatcttt tttacggaca aggcagaaga
    18541 atgtgagagc gaactgaagt ccttatttct gaatcatctg gaatattcat taataaaaga
    18601 tataacaaca gttaagccct gggatattta ttatgcgtta gctctcagtt tacgagacct
    18661 gctgatagaa agatggttgc gcactcaata tgaatatcgc aagcaggatg tgaagaaggt
    18721 ctattatctt tcgctggaat ttttgctggg gagaatgctt accaatagtt taatcaatct
    18781 ggatgtgtat aacgaagttt acgatatgtt gaaggaaatg ggtatttctt tggaggatat
    18841 tattgaactg gaaccggata tgggcttggg gaatggtggt ttaggacgtt tagctgcctg
    18901 ttttatggat tctttagcta cccaggcata tcctgcttat ggatatggta ttcgttatga
    18961 ttatggcatt ttcaagcagc agattgttca gggttatcaa gtggaagaac ctgataattg
    19021 gcgtaaaaat ggttgtccct gggaaatcaa tcgtccggaa ctaacttacc gtgtccgttt
    19081 tgggggcaag gttatcagcg agacctttcc cgatggcaga attgaacacc attggattga
    19141 tacggaagat gttctggcag ttgcctggga tattcctgtt cctggttatc aagtggacaa
    19201 tgtgaataat ttacgtttgt ggcaggcaac cagcactgcg gaatttgatt tggaatattt
    19261 caataatggc gattatgtaa aggcagtgga gaagaaaacc atttccgaaa acatcagcaa
    19321 agttctctat ccgaatgata atgttcattt gggcaggatg ttgcgtttac agcaggaata
    19381 tttctttgtt tctgccactt tgcaggatat ttttgcgcat tggaaacgct atcacaatag
    19441 ttttaataac tttgcggata aaattgccat tcagttgaat gatacgcatc cggctcttgc
    19501 tattccggaa atgttgcgaa ttttgattga tgaagaacga atgagttttg aaaatgcctg
    19561 ggaaatcacc aagcgctgtt tttcttatac caatcatact attttgcctg aagcgctgga
    19621 aaaatggggt attgctcttt ttgaagaatt actgccacgg catctgatgc ttatatatca
    19681 aataaataat gtagttatgg aagaagtggt gcgtttgtat cctggcgatt tagccaaaat
    19741 gcgtaatctt tccattattg aagaaaccaa cggaaaagct atcagaatgg ctcaactggc
    19801 aattcatagt tcgcatactg taaatggagt tgccaaatta cataccaaaa tcctctgtga
    19861 aagaattttt cccgatttgg ctgaattata cccgggaaag tttcaaaata agaccaatgg
    19921 catcacaccg cgtctttggt tgcatacctg taatccgcaa ttggcaagct taatttccga
    19981 atatataggt agtggttgga tcaccaatct ggaggaaatt cgcggtattg aaaaatatat
    20041 aaatgactct gattttcgca cttcttttgc cgaagtgaaa gatataaaca aaagccattt
    20101 aagcagatac atttatcgcg aaacaggaat acgcgtgcag gtaaattccc tttttgatgc
    20161 tcaaataaaa cgcctgcatg aatataaacg ccaattattg aatgtgatgg gcacaattgc
    20221 ccgttatttt cgcattaaag ataatcccaa tggtaatttt gtgcccagaa cggttatttt
    20281 tgccggaaaa gcagcaccag gatactttct ggcaaagcgt ttgattaaat tgataaacaa
    20341 cattggagaa gtggtaaata aagacccgga catcaaagac cgtttaaaag tcgtcttttt
    20401 gcctaattat tgcgtttctt tagccgaaag gattattcct gctgcagacc tctctatcca
    20461 aatttctact gccggttatg aagccagtgg aacgggaaat atgaaatttg ccttaaacgg
    20521 agctttaact ttaggaactt tggatggtgc caatattgaa atggctgaag agattggagc
    20581 ggaaaatatg ttcatttttg gcttgactgc agaggaagtg aaagagctta atcaaagcgg
    20641 ttacaatcct cgcagttatt atgaaagcga ccctgaactg aaaagagttg tggattcttt
    20701 aattgacggc acttttgagg aaggcgaaaa agacctcttt ttacctatct ggaaagcact
    20761 gatggaagat ggggatagct atttgaatat ggctgatttc cgcgctttcg tagagacctc
    20821 caataaagtt gatgagttct atttgaaccg tgaagagtgg ataactaagg caattctgaa
    20881 tgtagcacgt gtgggaaaat tttccagcga cagagcgatt aaagaatatg ccgatgacat
    20941 ttggggcatt aaacccctgc ctttggaact gaacaacgga aataattcct gatttttatg
    21001 gcttggataa ggaactttat cctctttgct ttttggggat ttttgtgcct tgctttaagg
    21061 gcagaaattg taattaacga ggtctgttac gatccagttg gcagtgatga gggctgggaa
    21121 tggatagaac tttataataa cggtaacaca agtgtgcagt tggaaggagc caaaatcctt
    21181 tccggaggca gttcttttac tcttcaatac acttttccct tttttgaatt gcgtcctcat
    21241 cgctatttac taattggagg agcgtcaata ttgaatgctc aactttacta taatttcagc
    21301 tttcaaaacg ggggaagtga aacggatggc attcgctatg tgagtcctga gggaacttat
    21361 accgatacag ttctttatga ttctcccaat accaatcagt tatttgatga tcataatttg
    21421 gcaggaacgc attttgctcc cgatgttccg gcaggttatt ctttggcaag aatttacgat
    21481 ggctacgata ccaataattg tgaaacggat tttatagcgg aagcaaaacc aactcccggc
    21541 ttgcccaatc ggattcattg cgattatgct ttaggaactt atagtgtttt gcgggagaat
    21601 aattatgttg agcttgacct ttggctgaaa aatttaagct ttcttgttcc gattttatct
    21661 gcagagttaa ccgttactca aaataatgag ttactggctc aacaggcaat tgctccaatt
    21721 ccagctttgg attcacttct ggtaaatctg gcatttttct gcgcttcttc gcctttaacc
    21781 gtaaaattat cgcttgagga tgatcctgat tccaccaata atgttttgtt agttccctta
    21841 actcctgaaa tggaagaggg catttatatc aacgagtttc tggctaatcc ggaatccgga
    21901 aatcaggaat ggattgaaat ttacggtgcc caaatttctc agggaaatta ttatattgca
    21961 gatagttcca cttcccaaat ccgtttttcc cttcctgcaa caagtggcta ttatgtaatt
    22021 tgcagagaac cggatgcttt aatttggcgt tatccagagt gtcctgccgc ttcaataatt
    22081 ttggctgaga gttggactta tctgaataat gcgggagatt gtctggtttt gaaaaatgaa
    22141 accggtactt tggattcttt gaattattcc ggagaggaaa taataaaagg tgtttcccgc
    22201 gaaagagtta ctgcggattc cactgtgttt tggcaaaatt gttatagtga aataggtgga
    22261 actccgggac ttcccaacag ctgtatgccg caaacagaac tgcccgattt gggaaaagtt
    22321 gttttatcag gaagcccttg taacgctaaa gcgggagaga aaatatctct tgcctacaat
    22381 tttccctctg ccaccaatag aattacctgc aatatttacg atttgaacgg aagaaagatt
    22441 tttactgtag cagattatgc cctggtaaat gctgcgggag ttctttactg gaatggctgc
    22501 aaacaggacg gcaattttgc cccgcgcggt ctctatattg ttttgtggga atcgcaaaat
    22561 gccggtggcg ggaaaattat gcgcaaacaa tttaccgctg ttttaaagtg ataacgaaca
    22621 ggaaaaaggg ttgagagggt gaagaaggtt tagagggttg agaattccgg aaggttgata
    22681 tcctcatcaa gctctcggaa aatttcctgc cgtcataacg atgttatgta aactatctct
    22741 tttgtccttg ctctcctata atcaattctc ctgcaataac ctgcaatata tatcgtaatt
    22801 ttcccgatca aaacaataaa aataaacatc tatttcaggg tgttgaggca aaaattcccg
    22861 cactgtttcc acagcaattt tgcctgcttc ttctttggga aaacggtata cgccggtaga
    22921 tatattggga aaggcaatgg acttaatccc tttttctaca gcaagttcca gcgatttttt
    22981 ataacaggat gccaataatt ccgcttccct aaagttaccc ccctgccaaa caggaccaac
    23041 tgtatggata acatattgtg ccttaaggtt ataacccttg gtaatttttg cctcgccggt
    23101 tttgcatcca cctaatgttc tacattcttc taaaagacct tttccggcag ctctatggat
    23161 tgcaccgtca actcctccgc cacctaaaag ggagctattt gccgcattta caattgcttc
    23221 gccctcaaaa agggttatat cgccatctct cagttctatc attttttcac ctcctttttg
    23281 caattgggat gtaaaattcc gttttgctac aaagaacata atccttgatg gctatacctt
    23341 caaactttgc cgaagcgaaa ttctaatgtt gaaaaccgct aacaaaagtg cctaatttcg
    23401 tcaatggaaa taagatatat tttttatcca gcaaacaaaa accgaaatct gatagttctt
    23461 ttttgctcat taaaaagacc ctccaattta agcgtttaac tccttattta ttagttggtt
    23521 cttaatcaac cccgtaacac taccgtaaca ctaccgtaac acttctgtaa cactgcagta
    23581 atatcgttac ttgatcgtta tagatttgct acaaatatat taccgggtgc agaaaggaac
    23641 tgaaacgaca accaaggcag tgttattatt ccttgacttt tttccaaagt atataaaaag
    23701 agaacaacat attaaacaaa ggagattgaa ccaatgatca actgcaaaat ttaaggcata
    23761 acagtccggg aaaatgctct tatcacattg ttaaaaagaa aattgctatg tcctttttgc
    23821 actatcggga agcccaatag ttgttcttat tttccaagtg gcatttgcat attttgccat
    23881 tcttgagata gcaggaatcc ctacaattaa aatggattcc gcaacaaatg cgaaatgaca
    23941 agcggtcttt ttaccgtcat tcttgtgttt ctttgtcctt cctgcaaaaa gcggaattca
    24001 gataagggta acttcttctg gcatttaact ttaaagaact atctcaccct ttctctcata
    24061 aagaaagctg ttatgcctgc ccgctaagaa aactgctaaa aacaattaaa ggagaaaaaa
    24121 tgcaggcaat acggctaaaa aatatacact tcacctatcc cgatcaatat caggaaattc
    24181 tttccgggat tactctggat atttgtgacg gacaaaaaat cgctctcatc gggaaaaatg
    24241 gttgcggaaa atctactttg ctaaaaatta ttaggggtga actgcagcca acttcgggtt
    24301 ctatttccta tcctatagct gttcccaaaa tcggttatct gcctcaagac ctgatgatta
    24361 gcaaccaatt aactgtgcgt gattttttat tgcagggaga tattaatagc tataatatta
    24421 ttagagaaat caataccctc tccgcggaaa aaaacctgtg cgttgaagat ggcttgcatc
    24481 ttgctgaatt gtggcaaaat tatgagcacg gcaatgtttt attctgggaa caaaatgtgg
    24541 atgaagtaat taggaaaatg gacttgaatg ccctggcaaa tcaaagctgt aacactcttt
    24601 ctgcgggaga aacaactcgc gttcagcttg cctcgctttt attgcaaaat ccggatattt
    24661 taattttgga tg
//
LOCUS       DAOPFL010000017        24021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2162_length_24021_cov_91.264458, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000017 DAOPFL010000000
VERSION     DAOPFL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 24021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24021
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2162_length_24021_cov_91.264458"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..300
                     /locus_tag="PLG69_02440"
     CDS             151..300
                     /locus_tag="PLG69_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42400.1"
                     /translation="MKRSHHLFITIWLMAMATLLGAQETLQIVSVDQLPFSVQMVSDA
                     IVMAS"
     gene            591..2039
                     /locus_tag="PLG69_02445"
     CDS             591..2039
                     /locus_tag="PLG69_02445"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPI42401.1"
                     /translation="MKEKMTLDYDQFRNLVTDETFVKLRQMTKKFNIFEVMKSTHTEI
                     RHSNILIWLLDPYENHGCGDAFLRQLLLTISQKPSLQDLGFYNLYLLDIESVKIDPEW
                     VPRKEGAKESSDDQENQLAPDRKYRDRIDILINIKLKNQINLKSTSKNYTRIVIPIEN
                     KIRSKESVKDSGTQLKVYSDALTKVFNKDVRIIPIFLTPDGSDPTDSAWIKIDYEEVI
                     DIINTVFKSYRNDMSSDKQMMIQQYLDLLRDHIVGGSDPEIVKLCRILQSKHMLAMDE
                     VRRLTTSSKDKETEKHKLCQQITIRNKSVFKAYKKWQYGLRERTSDLLLTWMNNHMES
                     LGFQINTKKKSSIEFVSPEMQKLNRELFGKDNTIVLIFVIKLNSYISIVMQINSHPNQ
                     ELRKSVYSIFQKDNSELFRSTDTEKISETYTRIYTHKILTPEDTFIMSSDKEVLNSIE
                     NKLEEFFAENGVYKQIQRFVRDHMEEFLALKD"
     gene            complement(2301..2918)
                     /locus_tag="PLG69_02450"
     CDS             complement(2301..2918)
                     /locus_tag="PLG69_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42402.1"
                     /translation="MTQDKDYTIIGIEGDQIDIMVEGMSVIGRITLRDRRNISVEIIS
                     PFSGISEQSGCIPLLGLQVHNFLGKAGDERAASLLCALYRFCRFVDEHKERLLEALHN
                     YKYKVAYAKHFSPEARELAQRRTTTLENLLAIRKELKEGKIDNIQYQRRIRPLNKVMK
                     LLSKEKEIDLDAIFDECFNHFWNTPVWELSRETVLNYLEEIIKTK"
     gene            complement(2946..4868)
                     /locus_tag="PLG69_02455"
     CDS             complement(2946..4868)
                     /locus_tag="PLG69_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42403.1"
                     /translation="MKSIVRYLEQLQDVEDKRKVVTYIHDLLDKEELCDEASDSETES
                     EWQNSSHSEVKANLSDSPSHHAVMRSLQCSIAKSPLLDAYQLQPNDLMLIARICQKTK
                     ANCEQDFNVTEICEMMPGIEDNLDKQYAAIVDLIDREILSTPHLMGVDYHYDLRALYG
                     TGLRLNGLLWNLILGKDPIKNGLKSFGKGLAKSDTAMDAIMKMIGVLFMHYPELTGDF
                     STNNIACYGRNVSLVLDHYLHALINAKGSAKWTEFCKKNRLNSFWQKCLLLIYYFNRE
                     TANQISPATLASLLAKSPKERKHFIDILAKDNLLCRKGLIEPPFPIFAVYMMELTSNA
                     VTALHGETEKAAEEAGKVDRSLSNSTYLSCIYPTQKLEQLILDQPTRELIVTIVQRLQ
                     NPQRDEFTEWGLMGASLTGDKDVQQGINILLHGVPGTGKTFIAGVIANELKRPLIQIN
                     ANAIRGSLYGESEKQARELFQEMRAAVKRYSPVFLLNEGDQLIHQRINSPDRSVDHTE
                     NTIQSVFLEELESFPGILIITTNLADNLDLAMSRRLHYKLEIKVPDYQARIALWRMHL
                     PESIPGASDIAVEGLANDFTFTGGQIRLVVQNACHEAYLRGKTAKLTLADLRKYAILE
                     SGTNFETPKRAIGFAL"
     gene            5379..5990
                     /locus_tag="PLG69_02460"
     CDS             5379..5990
                     /locus_tag="PLG69_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42404.1"
                     /translation="MKERVCLILGLAAILLMLSACESGGKLQVFNRCSYPAYIKVENM
                     EQKTIPAGESVSINIDTDTQNIFTGEVKKKLPIWLVGETFSIFDEYQNAYLDSTWIEV
                     KAGKTYSIYLNPNRASVKVINNSNQIISWAEIWQHSPLTYYQVGVMYNILPGESRFLR
                     VDYGNKYYYQVEIYLPDESHYTFGDSENILAVDEQYVVNFNPN"
     gene            6039..8837
                     /locus_tag="PLG69_02465"
     CDS             6039..8837
                     /locus_tag="PLG69_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPI42405.1"
                     /translation="MKRIYLGLILLCCILTLGAILPPEYASFPLEQDPDVISGTLDNG
                     LKYYIKQNAKPEKRLELRLFINAGSVVEDDDQLGLAHFVEHMAFNGTKNFPRTEMVDY
                     LTSIGMGYHNGLNGGTSYDFTVYEFKLPTDDEAKMRKGISILSDIAWQVSFEPAEIER
                     ERGVVQEEWRLGQNAQRRIQDQIDKVRFAGSRYAERNPIGTIENLKNFKHESLIRYYQ
                     DWYRPDLETVFIVGDYDPQKLEGLVKEYFGVIPKRENPRPRINYPVPDNIEPRAVTVL
                     DKEQPYTMIRSTWKVKATPVTDLGSLYNEMKQDLFFTMINARLEELSQQPDPSFSYAY
                     MFNATWLKGFNATDCFMLANEGRSEDAFRTLITELTRVRQHGFQPGEWERAKQIMIRQ
                     AEKWVADKPTMDSEDVIWELLDAVMSEDTILSAETYEQMLKGLIYEIALSEVNDIVDD
                     VITSENLTLSLAGTDKPGAKYPSREDLLNIYQQSIAQELEPWEDITVNEPLLETIPIP
                     GKITKEKVFPKSGIKQWVLSNGITVYSKKTDFKADEVILIAQSPGGKAKLKPENYKSA
                     DLLSQYFNVSGFGNFDAPALQKALAGKIVYVYPTIYSYSEGWRGSCSPQDLELMFQML
                     YQYNYAPRYNEEAFSAAVASTRARVQNYLLEPSNAFFDTLNVLMFNNHPLKRNLHPED
                     LDSVTLKQLKDIFQDRFGDYTDFTFYVVGNFAEDQLKDYCQTYLANLPAKGRKEKMTD
                     AGVRAFNGKKEISFHKGTERSFVSNVTNNKASFSPQNNVQQSALQMIAYEKLRENVRE
                     NMSGAYVVEIQSFYDFLPKPGVFTLTLMGCDPERAKELNAAIFATLDSLKNGLFADKY
                     IESTKTTLHKRYEENIKSNRYWVNNMSENISYGLPIDCFLDYPVLYDKLDKKAITKTA
                     KQYYVFDKSLLSVYMFPE"
     gene            complement(8851..8976)
                     /locus_tag="PLG69_02470"
     CDS             complement(8851..8976)
                     /locus_tag="PLG69_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42406.1"
                     /translation="MKLSPFILVLAYNFHLPSWNHNPQFVNTYNRVFTNFQIEKL"
     gene            complement(9023..11587)
                     /locus_tag="PLG69_02475"
     CDS             complement(9023..11587)
                     /locus_tag="PLG69_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI42407.1"
                     /translation="MKRLFLILFITTLISLNFAIQVSGNQSGVWSPVNNPYEVIGAIT
                     VPASDSLIIQAGVEIHLLGSYQITVEGVLNAYGTEVDSIRILNMQANPTALWPGLRFE
                     NLQHTSHLNYVYLEYATYGVRTINSPIAISHCHINLCEKGMEFYGIGSAVPLPNVVEY
                     CLIENCTQNAILVTSNSNAYIRYNEIRYNGTGAQFRAAIQMGNQSGVNQCNPNILYNH
                     IHHNYKQGISAWDIASSGSINPQILHNIIEYNYTGIYLLQASGYVADNQINYNFIPGD
                     MNSGAGVMVSGVTSVPYFERNHIEGNYTGFYITNNGKPVLGDLTLNHIWAQGENTIIN
                     NIDVNNHNNSVYCDAYPNANFIIMAENNNWGFATEAEIALTINDHNDNSALPTVDFEP
                     FIIPLQNTTITGSYIYTGTAQIANARLELIAVTNGIVQVSIPLTTADFSVQANISEDF
                     YAQVVLTETGTGKELYGCAGGYINPGVFSPAPGNSVNIGIVEVTDIPLSRYEYVGEPI
                     QEGNLTLYPLLTGWGLYSYDKLDWVYPAGDYLYLKRNIWRTPAGEVTTELPLGTVYKK
                     YLNINAGDSWQQTEVIYPGNITFTTDVQVTNCVTDISTPAFLLFTRKDSFGYVVDKRI
                     KGIAEELFHYQNHYNIARENIIYLPGNNDPLTTGNVTLFMPEPLSENPSFLAFEPDID
                     DQIHLFWQAPAQGNFSWTFYRIYKNGALIGEIPFSQSDYIVTDFNPQITTYFYVTATD
                     GINESEPSNCVTVYYVGNEDLIQKPFSVDVYPNPVSFTSGSGLIVEVKNAENSSALIE
                     IYNLKGQLVFRKNLQDEKNFLWKGIDNRGKRSSAGIYFLKIKVKGEKPITRKIMVL"
     gene            complement(11752..11922)
                     /locus_tag="PLG69_02480"
     CDS             complement(11752..11922)
                     /locus_tag="PLG69_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPI42408.1"
                     /translation="MAVKIDKETCIGCGACIDVCPVSALKLVNDKAECDESLCIDCGA
                     CIDTCPVQAISQ"
     gene            complement(12076..13293)
                     /locus_tag="PLG69_02485"
     CDS             complement(12076..13293)
                     /locus_tag="PLG69_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI42409.1"
                     /translation="MSIVGIIRIAFKSLARNKTRTFLTMLGIIIGVAAVITMLAIGQG
                     AKKIVEDQISSMGTNVLMVTVNFTRSSTNVRQAAGSGSLLELDDVFAIRSQVEDALYV
                     SPVYNSFAQLKFESQNWRTGVMGVDTDYFYIRNMNPEQGELFYSSDVENGAKVCVIGK
                     TVAENLFGDIDPVGQIIRIRNIPFTVKGVMESKGQNVMGADQDDSVWAPYTTVYTRLL
                     GRRWRNMMIIVSAVSKDKIAKVQQDILDLLQSRHRGTTSEEFLVRSQTDIAETASKVS
                     DTMTFLLASIAGISLLVGGIGIMNIMLVSVTERIKEIGIRMAVGAGKRDVLLQFIIEA
                     VTISILGGFIGILLGFGASKIVGNAMKWSVVVTPFSVLLSVGFSMAIGIFFGWYPASK
                     AANLNLIDALRYE"
     gene            complement(13290..14042)
                     /locus_tag="PLG69_02490"
     CDS             complement(13290..14042)
                     /locus_tag="PLG69_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42410.1"
                     /translation="MPNLLIRTENLTKIYQMGEVQVHALRGVSLQIKEGEFVAIMGAS
                     GSGKTTLMNLLGCLDLPTSGEYYINDIGVHEITPAELTKIRNQMIGYVFQNFYLLPRT
                     TALENVELPLLYRNKMTHHQRLQKAMEALSTVNIEDRAKHYPNQLSGGQQQRVAIARA
                     IVNDPVFLLADEPTGNLDTRTSLEVMTIFQELHKNGKTILLVTHEHDIAMYAHRLITF
                     RDGRIIADVPNSNPHDAAYDLANLPKLPGDEE"
     gene            complement(14044..15339)
                     /locus_tag="PLG69_02495"
     CDS             complement(14044..15339)
                     /locus_tag="PLG69_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPI42411.1"
                     /translation="MKKYLKYIIIIALILIAIIVIISLRKAKNKPEFRTTSPSFGNIR
                     EVVTATGSLNPYVLVSVGTEVSGKIDKLYKDFNDKVKKGELLAKLDTEILATNLEAAK
                     ADLAKAKTALDQAKLDYDLQAELLEKKMTPEYDAKKALFAYQTAQQNFNNAQLTLQRA
                     QKNLANAYITSPINGIVVSRDVDEGQTVAASMSSPTLFKIANNLDQMQISANVDEADI
                     GKIKVGLPVEFSVDAYPSENFSGNVKQIRLNPTTESNVVSYSVIIDAANPEQKLLPGM
                     TTNVTIIIQARENVMRISETATRFKPSKEIWEQLGYKWSDDLLLKAQKEAYASLAKAK
                     TIPAKTDSTNFNQKKYGKTKQEFSSKTISSSGKAALIWVLEDNQPKPKGIRTGISESG
                     YIEVIEGLTGNETIITGVNTKSGSETNNGTQGGPGMRHF"
     gene            complement(15376..16620)
                     /locus_tag="PLG69_02500"
     CDS             complement(15376..16620)
                     /locus_tag="PLG69_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPI42412.1"
                     /translation="MKVITVILMLLPLLLAAQSYTLEELITYGLENSFSVQKSALSYQ
                     SSKSTLNTSKWNLLPDASVSGGIKKDFDPAVNTDDVTSSAGLSIGKTISLNDAAYFNY
                     LYASLDTKTANMKLEQTRRAYAYEVFSAYLKVLSSQKQRSSLEENLQIQIRVWEQSKI
                     LLQLGKTTPFEVKQNEIAVLNSNISLMKLDNTIANARKELFALVQMQDENYPLTELEL
                     NIQKEIPAFNTEKNADLNILNQELKRLNLQLKQNKLDDFPRLNLAYNFDRSVSGADFD
                     FDRYNTNHGISLTLSYPLFNIGENAESRTRTKISRQLSQVAIEEKKDQLQRDYDSALR
                     ELQYLKRMNELYSEQLAQSREQITQAEERYRLGLIELLELDKTRTNYIETDISYNTNL
                     YSIIAKQEEINYLLSEPVLGKW"
     gene            complement(16906..17352)
                     /locus_tag="PLG69_02505"
     CDS             complement(16906..17352)
                     /locus_tag="PLG69_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42413.1"
                     /translation="MKVNFQYGLAGYTGKADGLVFCFDRKSGIVYARKRVYPRLTEEN
                     ARIGNISHNIFSLKPSEEYKDDLRLYLMGYNGLRANSHKPLRSWVNLYHRLMANMAKK
                     NPEIDLKTLTRQQIFADNLPCKSIKSAVEAGILPPVNGYERMTALI"
     gene            17808..18806
                     /gene="argF"
                     /locus_tag="PLG69_02510"
     CDS             17808..18806
                     /gene="argF"
                     /locus_tag="PLG69_02510"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPI42414.1"
                     /translation="MSFNLKNRNFLTLMDFTPREIQFLLDLAIQLKKAKYAGTEQQKL
                     KGKNIALIFEKDSTRTRCAFEVAALDQGAHTTYLGPTGSQLGKKESVADTARVLGRMY
                     DGIEYRGFGQEIVEELAKYAGVPVWNGLTDQDHPTQVLADFLTALEHLNKPLNEMVFV
                     YCGDGRNNMANALMIGACKTGMDFRIVSPKTLFPEQNLMHKCLKVAQETGAKITLTDN
                     IDAGVKDADVLYTDVWVSMGEPDSVWEQRINLLKPYQVNGEMLKKTGKSTTIFMHCLP
                     SFHDLKTRIGKDIYEKFGLTAMEVTDEVFEGPHSVVFDEAENRMHTIKAVMVATLG"
     gene            19169..19996
                     /locus_tag="PLG69_02515"
     CDS             19169..19996
                     /locus_tag="PLG69_02515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPI42415.1"
                     /translation="MNNNEYELYLGDALEILQTIEKESIDLIVTSPPYADSRTNTYGG
                     IKPDEYNEWFLPITQELLRILKPTGTFILNIKEKVVNGERHTYVIELILNMRKQGWLW
                     TEEFIWHKKNCYPGKWPNRFRDAWERLLQFNKNKFFKMYQEEVMIPIGDWAEKRLSNL
                     SHTDKIRDTSKVGSGFGKNVSNWVGKDKVYPTNVLHLATECGNKNHSGTFPYALPEWF
                     IKLFTRPGDVVLDPFMGSGTAIFAALNMGRKAIGIDINPEYYNLVLGKIQNQQQVLF"
     gene            20009..20365
                     /locus_tag="PLG69_02520"
     CDS             20009..20365
                     /locus_tag="PLG69_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HPI42416.1"
                     /translation="MIDENDLNEYVEKHIEIFHNNRIMNISNLKLTNILKHKNPYLFR
                     AKNSPMFLLNIGHLYITTGPHMDSKDRFYVVLYKEQGGIPSENLDKFKTDLKNRDVFW
                     GKGVGIDDLEFLELVQ"
     gene            20460..21911
                     /gene="dgt"
                     /locus_tag="PLG69_02525"
     CDS             20460..21911
                     /gene="dgt"
                     /locus_tag="PLG69_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HPI42417.1"
                     /translation="MNQLKPLEHMKADPKDAICKNPPNGDDFRNIFQRDRDRIMYSKA
                     FRRLAGKTQVFFAGHDDHLRTRLTHTLEVSQIARTISSKLGLDLDLTEAIALGHDIGH
                     APFGHVGERVLNYFSNGCELYKGFNYREKTDSIVDNGVETLPIEFRGYKHNWQSVRIA
                     VNLTRHRGELGLDLCRNTIFGILYHTALSYGKCPHRSAGYCKLRERSKERKCGDPILH
                     LDYYTNNYGEYQDLRYYSVESLVVALSDEIAQRHHDIEDGIRANLISEEELKLKLKDF
                     IKLDVPEIHEKKHVCSNEKEDFITILSGLVVHSYVKKTINYLTEQLGERKYKNIRELI
                     QSTDLDTVMKNKDIYKVVEGDEQLHRFLKERMLSSHIAQVMDGRADFLIRRLFKAYIS
                     NPQQLPDNTIRIMMKTYRDYRQSKGYGNVAYSNVDFDEIPIGEIRKHLAKLQTRGRFS
                     FRAIMLRTICDYISGMTDRRAISEYQNLYGIHN"
     gene            21931..23409
                     /locus_tag="PLG69_02530"
     CDS             21931..23409
                     /locus_tag="PLG69_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPI42418.1"
                     /translation="MVQKSINLTGEQKKVLFLPATNPIQIKGVAGSGKTTIAMYRAKY
                     LLDTYSDLFREAEIMIFTYNNSLTQYIKSLLPYVIGGYQENSDARNPSTQGLRVKVST
                     FHKWSYGFLSRNGVFNDYTQMERSEHNEILCHAIKETRKSFQGARILNKPLNFFDEEL
                     SWIKGQLFKTLNDYLSTARIGHGTKDRVDKNDKAVIWNVYETYCLEQISRKKIHFDDH
                     ASYCLNLIEKHNIQPEYTHIVVDEAQDMTKAQIMVIKSLVKPSTNSLTIIADTAQRIY
                     KSGFTWTEVGINVRGGRTVELKKNYRNTKEIALAANSLMSHDLEDDDLTAMDIDSITS
                     HGDKPVLIYYSDAEGANRALLEKTKEAKLKGGSIVVLHRYRNMLDYYSKLLKNCGIAT
                     EVISPDNPADYASNSVKICTLSSVKGLEFDHVIIMELSSMIIPSPDGFGQDNDEYHIT
                     TERKLLYTAMTRAKQSLFLFVPQAYPSMFIDEIDKQFLTVIR"
     gene            23418..>24021
                     /locus_tag="PLG69_02535"
     CDS             23418..>24021
                     /locus_tag="PLG69_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DarT ssDNA thymidine ADP-ribosyltransferase
                     family protein"
                     /protein_id="HPI42419.1"
                     /translation="MFTEKPDSGQFKQEIKRRGITRLIHFTTTINLLGIYESGFLYSR
                     KELEDLDINMTDILDYIQFNDQIRYDDKTYINLSIERPNSYLFKRFIDNTRDKPYISW
                     CVLSIDPKYIYEQGTLFSITNASNSYNKKNVGITGDFHKFQMMFDDSLPIVSSRRTIL
                     VYRTGLPDAYTTHEQAEVLVKIPIPVADIISVSFPTQEKLA"
BASE COUNT         7385 a         4589 c         4843 g         7204 t
ORIGIN      
        1 tgtttgacaa gatttctatt gagataacct tgatttgttt gagaggatag agataaaaat
       61 aggaagtaaa agaaccattg acagaaaaca taaattataa cataataaga ttataaggac
      121 tttaaaaata aaaatataag gaggtggaca atgaaacgga gccaccatct ttttataact
      181 atctggctaa tggctatggc taccctattg ggggctcaag aaacgctaca aatagtaagt
      241 gttgatcaat taccctttag tgtccaaatg gtctcagatg ccatagtaat ggcatcttaa
      301 aacaggaatt tggcttagga aggactttgc aatctgtaaa agtagctcaa gaattagctt
      361 cagatgcaat ctacatctac aataataact gattgcttag cgctctgatg tataaaacgc
      421 cttgcgaggt gcacgattca ctctaagtta agtaataacg acgagcaccc ttcttgcgtg
      481 cacatcgtcg tttttgcgtc tataaagtgt aaactatttt aggacttgac acttttgaga
      541 tgatatgaaa atgggattaa taaaaataac ctgcataagg agagacaaca atgaaagaga
      601 agatgaccct tgattacgat caattccgaa atttggttac agacgagaca ttcgttaagc
      661 ttcgtcaaat gacaaaaaag tttaacattt tcgaagtaat gaagtcgact catacagaga
      721 ttcgtcattc aaacattctt atatggttgc ttgatcccta tgagaatcat ggatgcggag
      781 atgctttttt acggcaactg cttctgacta tttctcaaaa gcccagcctt caagatttgg
      841 gtttttataa cttgtatcta cttgatattg agagcgtaaa aattgatcca gagtgggtgc
      901 caagaaagga aggagcaaag gaatctagcg atgatcaaga aaaccagctc gctccagata
      961 gaaaatacag ggatagaatt gatatcttga ttaacatcaa gttaaagaat caaatcaacc
     1021 taaaaagtac ttctaagaac tatacccgca ttgtaatccc catcgaaaac aagatccgaa
     1081 gcaaggagtc tgttaaagat agtggaactc agttaaaagt ttatagcgat gccctaacga
     1141 aggtatttaa taaggacgtc agaataatac ccatattctt gactccggat ggctccgatc
     1201 ctacagattc tgcctggata aaaattgact acgaagaggt aatagatatt ataaacactg
     1261 tattcaagag ctatagaaat gacatgagct cagataaaca gatgatgatc cagcaatacc
     1321 tagatctttt gagagaccat attgtcggtg gttctgatcc agaaatcgtg aagctatgta
     1381 gaatcctgca atcaaaacat atgctggcaa tggacgaagt tagaagatta accacctcat
     1441 caaaagataa ggaaactgaa aagcataaac tttgccaaca aataactatt cgcaataaat
     1501 cagtttttaa agcttataaa aaatggcagt atggattgag agaaagaaca tcagatcttc
     1561 tgttaacttg gatgaacaat catatggaaa gccttgggtt ccagattaat acaaaaaaga
     1621 aatcctcaat agaatttgtg agcccagaaa tgcaaaagct aaatagagag ctcttcggca
     1681 aggacaatac tattgtctta atctttgtta ttaagcttaa tagctatatc agcatcgtaa
     1741 tgcagataaa ctcacaccca aaccaggaac tccgaaagtc agtttacagt attttccaga
     1801 aagataactc tgaactgttt aggtcaacgg atacagagaa aatatcggag acatatacca
     1861 gaatatatac acataaaata ctcactccag aagatacgtt cataatgtcc agcgataagg
     1921 aagtgctcaa ttctattgaa aacaagctgg aggagttttt tgctgaaaat ggagtataca
     1981 agcaaatcca gcgatttgtt cgggatcata tggaggaatt tttagcatta aaagactaaa
     2041 ctccatctta tatggcgtcg cagtcggcga tgtccttagc tttccagccc aatttgagtc
     2101 tcgatctgag cgcaagaagc tcccaggtgt cggagtgggt caatacaaag atgaaaatgg
     2161 acagacctac tccttgggca atgatctcac tggcttgtgg tcggacgaca cttctttgac
     2221 cttatgtctg tcagagttgc cgagactgac aagggtgact taacagaact gcactccctg
     2281 ggaagcagga tcgttcaagt tcatttagtt ttaatgattt cctccaaata gttaagcaca
     2341 gtttctcggc tcagttccca gaccggggta ttccagaagt ggttgaaaca ttcgtcaaag
     2401 atggcatcca ggtcgatctc cttctctttc gagagtagct tcatgacctt gttcaatggc
     2461 cttattctgc gctggtactg gatgttatct atcttgcctt ctttcagttc ctttctgata
     2521 gccagtaagt tctccagcgt agttgtcctt cgctgcgcca gttctctggc ttccgggctg
     2581 aaatgtttcg cataggctac tttatacttg tagttatgca atgcctccag caatcgttcc
     2641 ttgtgctcat ccacaaagcg gcagaagcga tataatgcgc aaagcaagct tgcagccctc
     2701 tcgtcaccgg cttttcccag gaagttatga acctgcaaac ccagcaaagg gatgcagccg
     2761 gattgctctg agatgccgga aaagggactg atgatctcca cagatatgtt tctcctgtca
     2821 cgcaatgtga tcctacctat tacagacata ccctcaacca taatgtcaat ctgatctccc
     2881 tctataccta taatcgtgta gtccttatct tgtgtcactt ttcctccttt gatgtatatt
     2941 cttgcttaca atgcaaaacc aatggccctc tttggcgttt cgaagttagt gccgctctct
     3001 aggatggcat atttacgcag atcagccagg gtcaatttgg ctgtttttcc ccgcagatag
     3061 gcctcatgac aggcattctg caccaccagc ctgatctgtc ctccggtgaa ggtgaagtca
     3121 tttgccaggc cttccacagc tatgtccgat gcccctggaa tgctttcggg aagatgcatt
     3181 cgccacagcg ctatccgggc ttgataatca ggcactttga tttccaactt atagtgaagc
     3241 cgtcgggaca ttgccagatc gagattatca gccagattgg tggtgatgat caggataccg
     3301 ggaaagcttt ccaattcctc cagaaacacg ctctggatgg tattctccgt atgatcaacc
     3361 gatctatccg gagaattaat gcgctgatgg ataagctgat ctccctcgtt tagtaaaaaa
     3421 acaggagaat agcgcttcac agccgcacgc atctcttgaa agagttccct ggcctgtttt
     3481 tcactctctc cgtaaagcga acctcggata gcgttggcat tgatctggat caaaggacgc
     3541 ttcaattcat tggcgatcac gcctgctatg aaggtcttgc cagtaccggg aactccgtgc
     3601 aagaggatgt tgattccttg ctggacatcc ttgtcacctg tcaacgaagc acccatcaag
     3661 ccccactcgg taaattcatc ccgctgagga ttctgcaacc tttgcacaat ggtaacgata
     3721 agttcacggg ttggctgatc caggatgagt tgctccagct tttgggtggg atatatgcaa
     3781 gaaagataag tggaattgga aagcgatctg tccactttac ccgcttcttc tgctgccttt
     3841 tccgtttcgc catgcagggc tgtaacagca ttggaggtca gttccatcat atagacagca
     3901 aaaatgggga aaggaggttc tatcaggcct tttctacaaa gcagattatc tttagcaagt
     3961 atatctatga agtgcttgcg ctctttaggg ctcttcgcca gtagcgatgc aagagtggca
     4021 ggggaaattt gatttgctgt ctctctattg aagtaataga taagtaacag gcatttctgc
     4081 cagaatgagt tcaatctgtt cttcttgcag aattccgtcc atttggcgga acccttggca
     4141 ttaattaaag catgtaaata gtgatctaac accaagctta catttctgcc atagcatgct
     4201 atgttattgg ttgagaaatc gccagtcagt tccggataat gcataaagag caccccgatc
     4261 atcttcatta tagcatccat tgcggtgtcg gatttggcaa ggcctttgcc aaaagacttg
     4321 aggccattct tgatgggatc tttgcccagg atcaggttcc agagcagtcc attaagccgt
     4381 aagcctgtgc catatagtgc tcgtagatcg taatgataat ccactcccat taagtgaggt
     4441 gtactcagga tttcccggtc gatcagatca acaatggcgg catactgctt atccaggtta
     4501 tcttctatgc ccggcatcat ttcgcagatc tcggtcacat tgaaatcctg ctcgcagttc
     4561 gccttggtct tttggcagat cctggcaatt agcatcaaat cattgggctg caattgatat
     4621 gcgtcaagca gcggggactt tgctattgag cattgtaaag agcgcataac ggcatgatga
     4681 ctggggctat cactcaagtt agcttttacc tctgagtggg aactattctg ccactcactc
     4741 tcggtttcag aatcagatgc ctcatcacaa agctcttcct tatccaggag atcatgaatg
     4801 taagtcacta ccttgcgctt atcttcaaca tcctgcaatt gctccagata tctcacaata
     4861 cttttcatat cacttacctc cattctattt ataccttatt ataactttcc gatgatgaat
     4921 ttttgaaata tttttccttc aaggtatctt ttagagtacc gctatgatag acagtggtgg
     4981 tatgatatac acctacaaca agattactca gttgctgcac ctacaatctc ttcaagctat
     5041 tgatcaactc tatctactgc gacttgataa taatcaccct aatccaaggg atcgccattc
     5101 tcgtttgcag atatcctgtc agaattctaa attttcggtg atcgtatttg cagatatcca
     5161 gtcaaaaact atcaaaatgg gtcttggaaa ggatgagtaa gtatgtcata cgatgcgaca
     5221 gtttgcagtt ttccctgcac aatctacagt tttcgttgtc accttgcaat ttatactacc
     5281 tattataaaa acaggagtgg atatggtcaa ccaacaattt ccttgacaca ttgtttgcct
     5341 gataaaatgg ataattattg taagaagagg tgagaataat gaaagagaga gtttgcttaa
     5401 tcctggggtt ggcggctatt ctgttaatgc tttctgcttg tgaatccgga gggaaattgc
     5461 aggtttttaa ccgttgttca tatcctgctt atattaaagt ggaaaatatg gaacaaaaga
     5521 caattcctgc aggggaaagt gttagcatta atatagatac cgacacgcaa aatattttta
     5581 ccggggaagt gaaaaagaaa ctacctattt ggctggtagg ggagaccttt agtatttttg
     5641 atgagtatca aaatgcttat ctggattcta cttggattga agtgaaagcg gggaaaacat
     5701 acagtattta tcttaatcct aatcgtgcca gtgtgaaagt aataaacaac tctaaccaaa
     5761 taatcagctg ggcagaaatt tggcaacata gtcctttaac ttattatcag gtaggcgtta
     5821 tgtataatat tttgcctgga gaaagccgat ttttgcgtgt ggattatgga aataaatatt
     5881 actatcaggt agaaatatac cttccggatg agtcgcatta cacttttggc gacagcgaaa
     5941 acattttggc agtggacgaa caatatgtag ttaattttaa tccaaactaa agaaagcggg
     6001 caagctatca gggcaaaata aaaatacaag gagtaattat gaagaggatc tacctcggct
     6061 taatcctact atgctgtatt ctgactttag gtgcaatcct tcctccggaa tacgcaagtt
     6121 ttcctttgga acaagacccg gatgtaatat ccggcacttt ggataatggc ttgaaatatt
     6181 acatcaagca aaatgcaaaa ccggaaaaac gacttgaact acggcttttc atcaatgccg
     6241 gcagtgttgt ggaagatgat gatcaacttg ggctggcaca ttttgtagaa catatggctt
     6301 tcaacggcac taaaaatttc cccagaacgg aaatggtgga ttatcttact tctattggaa
     6361 tgggttatca caatggctta aatggcggaa cgagttatga ttttactgtt tatgaattta
     6421 agcttcctac tgatgatgaa gccaaaatgc ggaaaggaat ttctattctt tcggatattg
     6481 cctggcaagt aagttttgag cccgctgaaa ttgaacgcga aagaggtgtt gtgcaagaag
     6541 aatggcgtct gggacaaaat gcacaaagaa gaattcaaga ccaaatagat aaagtgcgtt
     6601 ttgccggttc tcgttatgcc gaaaggaatc ctatcggaac aattgaaaac cttaagaact
     6661 ttaagcacga gagtttaatt cgttattatc aggattggta tcgtcccgat ttagagaccg
     6721 tctttattgt gggtgattat gatccccaaa aattggaagg tttagttaag gaatactttg
     6781 gcgttattcc taaaagagaa aatccgcgtc cccgcattaa ttatcctgtt cccgataata
     6841 ttgaaccgag ggctgtaacg gttttggata aagaacagcc ctatacaatg ataagaagca
     6901 cctggaaagt taaggccact cctgtaactg atttgggttc tttgtataat gagatgaaac
     6961 aggatttgtt tttcacgatg attaatgccc gtttggaaga gcttagtcaa caacccgatc
     7021 cgtctttttc ttatgcctat atgtttaatg ctacttggct gaagggtttt aatgctacgg
     7081 attgttttat gcttgccaat gaaggtcgca gtgaagatgc cttcagaact ttgataacgg
     7141 aattgacgcg ggtgcgtcaa cacggttttc aacctggtga atgggaacga gccaaacaaa
     7201 taatgattag acaagcggaa aaatgggttg ccgataaacc gacaatggat tccgaagatg
     7261 taatttggga gcttttggat gccgtaatgt ctgaagatac aattctcagc gctgaaacct
     7321 atgaacaaat gctgaaaggg ctgatctatg aaattgcctt aagcgaagtg aacgacattg
     7381 ttgatgatgt tattacctct gaaaatctta ctttaagttt agctggaacg gataaaccag
     7441 gagcaaaata tccctcgcgc gaggacttgc tgaatattta tcagcagtca attgctcagg
     7501 aactggaacc ctgggaagat ataaccgtga atgaaccgct tttggaaaca attcccattc
     7561 cgggaaaaat aactaaagaa aaggtctttc ccaagtccgg aatcaaacaa tgggttcttt
     7621 ccaatggaat aactgtttac agcaaaaaaa cggattttaa ggcagatgag gttattctaa
     7681 tagcacaaag tcccggggga aaggcaaaac tgaaaccgga aaattataaa tctgccgact
     7741 tattatccca atatttcaat gtatcgggtt ttggtaattt tgatgctcct gctttgcaaa
     7801 aggcattagc aggcaaaatt gtgtatgttt atccaacaat ttactcttat tcagagggtt
     7861 ggagaggttc ttgttctccg caggatttgg aattgatgtt ccaaatgctc tatcaatata
     7921 attatgctcc cagatataat gaagaagcat tttctgcagc tgttgcttca accagggctc
     7981 gggttcaaaa ctatttgctg gaaccatcca acgccttctt tgatacttta aatgtgctga
     8041 tgttcaataa tcatcctctg aaaagaaatt tgcatccgga agacctggat agcgttactc
     8101 tgaaacagtt aaaagatatt ttccaagacc gctttggcga ttataccgat ttcacttttt
     8161 atgttgtcgg caattttgct gaagaccaat taaaggatta ttgccaaaca tatttagcca
     8221 atttacctgc aaagggaaga aaggaaaaaa tgaccgatgc cggcgttaga gcttttaacg
     8281 ggaaaaagga aatcagtttc cataaaggca ctgaacgcag ttttgtatct aatgtaacaa
     8341 acaataaggc atcgttttcc ccgcaaaaca atgtgcaaca atccgctctg caaatgattg
     8401 cttacgaaaa attgcgggaa aatgtccgcg aaaatatgag cggtgcttat gttgttgaaa
     8461 ttcagagttt ttatgacttt ctgcctaaac ctggtgtttt cactttaacc ttgatgggtt
     8521 gtgatcctga aagagctaag gaattgaatg ccgctatctt tgccactttg gatagcttga
     8581 aaaacggact ttttgcggat aaatatatag aatccactaa aaccactctg cataaaaggt
     8641 atgaagaaaa catcaagtcc aatagatact gggtgaataa tatgagtgag aatatttctt
     8701 acggcttacc gattgactgt tttctggatt atcctgtgct gtatgacaaa ctggataaga
     8761 aagctataac caaaaccgct aaacagtatt atgtttttga taagtccttg ctctccgtat
     8821 atatgtttcc ggaatagctt ttgttttagc tcacaatttt tcaatttgga aattagtgaa
     8881 aacgcggttg taagtgttta caaattgagg gttgtggttc caggaaggca ggtgaaaatt
     8941 gtaagccaaa acaagaataa agggtgacag tttcattttc tgccaccctt tttatttcta
     9001 ttacaaagaa ttcggaattt atttataaga ccattatttt acgggtaatg ggtttttcgc
     9061 ctttaacctt tattttcagg aaataaatgc cggcagaact gcgtttgcct ctattatcta
     9121 ttcccttcca aagaaagttc ttttcgtcct gcaggttttt tctaaatacc aattgccctt
     9181 tcaggttgta aatttctatg agggcagaac tgttttccgc atttttaact tccactataa
     9241 gacccgagcc agaggtaaaa ctaaccggat tgggatatac atctacgcta aaaggtttct
     9301 gaatgaggtc ttcattaccc acataatata ctgtaacaca gttgcttggt tcggattcat
     9361 ttattccgtc cgtagcagtt acataaaagt aagtagtaat ttgaggatta aaatcagtaa
     9421 cgatataatc gctttgagag aaaggaattt cgcctattag agcaccgttt ttataaatgc
     9481 ggtaaaaagt ccagctgaaa tttccctgtg cgggtgcctg ccaaaaaaga tgaatttgat
     9541 catcaatatc gggctcaaaa gcaagaaaag aagggttttc acttagcggt tcaggcataa
     9601 ataaagtaac atttcctgta gttaaagggt cattattgcc tgggaggtaa ataatgtttt
     9661 ccctggcaat gttgtaatga ttttggtaat ggaaaagttc ttcagcaatt ccttttattc
     9721 ttttatccac aacatatcca aatgaatcct tacgggtaaa aagcaaaaat gcaggggtag
     9781 aaatatctgt aacacaatta gtaacctgaa catcagtagt aaaagtgata ttgccaggat
     9841 agataacctc agtttgctgc cagctatctc ccgcattgat attgagatac tttttatata
     9901 cagtgcccaa aggcagttct gttgtcactt ctcccgcagg agttcgccag atatttcttt
     9961 ttaggtaaag ataatcacct gcaggataaa cccagtccaa tttatcatag ctgtataaac
    10021 cccaacctgt cagcaaagga tatagcgtta aattaccctc ctgaatcggt tctcctacat
    10081 actcataacg ggaaagagga atatctgtaa cttcaactat gccgatatta actgaatttc
    10141 caggagcagg agaaaaaaca ccaggattaa tataaccccc tgcacagcca taaagttctt
    10201 ttccggttcc cgtttcggtt aaaacaacct gcgcataaaa atcctcggaa atatttgcct
    10261 gaacggaaaa atcagctgtg gtaagaggaa tggagacctg aacaattccg ttggttactg
    10321 caattaattc caaacgcgca ttggcaattt gtgctgttcc agtataaata taactgcctg
    10381 tgatagttgt attttgcaga ggaataataa aaggttcaaa atcaactgtt ggcaaagcgg
    10441 aattatcatt gtggtcattt atcgttaagg caatttctgc ttccgtagca aacccccaat
    10501 tgttattttc tgccataata ataaaattgg cattaggata agcatcgcaa taaacggaat
    10561 tattgtgatt gttaacatca atattgttga taatggtatt ttcgccctga gcccaaatat
    10621 gatttaaagt tagatctccc aaaacaggtt tgccattatt agtaatgtaa aaaccggtat
    10681 aattaccctc aatatgattg cgttcaaaat agggcacgga agttactccc gaaaccatta
    10741 ctcctgctcc ggaattcata tctccaggaa taaaattgta gttaatttga ttatcagcta
    10801 cataacctga tgcctgcaaa agatagattc cggtgtagtt atactcaatg atattatgca
    10861 aaatttgcgg attgatagaa cccgaagaag caatatccca agccgaaatt ccctgcttgt
    10921 agttgtgatg aatatgatta tataagatat tgggattgca ctgatttacg cctgattgat
    10981 tgcccatttg aatagctgcc cgaaattgag ctcccgttcc attataacgg atttcattgt
    11041 atctgatata ggcattgcta ttggaagtga caagaatcgc attttgggtg cagttttcaa
    11101 ttaaacaata ctccactaca ttaggcaaag gaactgccga cccgatgcca taaaactcca
    11161 ttcctttttc acataagttt atatgacagt gagagatagc gataggggaa ttgatagtgc
    11221 gcactccata agtggcatat tccagataaa cataatttaa gtgactggta tgctgcagat
    11281 tttcaaaacg caaaccaggc caaagtgcag tcggatttgc ctgcatattt aaaattcgga
    11341 tactatctac ctctgttcca taggcattta atactccctc cacggtaatt tgataggagc
    11401 ccaaaaggtg aatttctacc cctgcctgaa taattaaaga atccgatgca ggaacagtta
    11461 ttgctccaat tacttcgtaa ggattgttta caggagacca aactccgctt tgatttccgc
    11521 ttacctgaat ggcaaaattc agggaaatga gagttgtaat aaaaagaata agaaataagc
    11581 gcttcatttt aatcacctcc tctaaagttt gatgttattg ctttactctt cgctaaatta
    11641 tactcatact tgaaaatata ataaaatatt agaaattgca ttccgcgtgg caaatcggaa
    11701 tttggcatcc cgatttgcca ctttgggaaa ttataactat ttttaattca gttattgtga
    11761 aattgcctga acaggacagg tatcaatgca agccccgcaa tcaatgcaca gtgactcgtc
    11821 acattctgcc ttgtcattta ccaattttaa agcagaaaca gggcatacat caatgcaggc
    11881 accgcatccg atacaggttt ctttatcaat tttgacagcc attttgtgtc tcctttcttt
    11941 ttttcttcat agttttaggg agttgagatt cgtcaagcaa aatatcactt gcaggcgagt
    12001 tttctttgtt tatagaactg ccaaatttag gtttacaatg ttactttggg aaattggggc
    12061 ttagttttta attgtttatt cataacgcaa ggcatcaatt aaattcagat ttgccgcttt
    12121 ggaagcagga taccagccaa aaaagatacc tatagccatt gagaatccaa ctgaaagcag
    12181 aacggaaaaa ggtgtaacta ccacactcca tttcattgca ttacccacaa ttttggaagc
    12241 tccaaaaccc agaaggatac ctataaaacc acctaaaatg ctgattgtaa ccgcttctat
    12301 aataaattgt aacaggacat ctctttttcc tgcgcctaca gccattctga tcccaatttc
    12361 cttgatgcgt tccgtaaccg aaaccagcat aatattcata atacctattc ccccaaccaa
    12421 aagagaaata cccgcaatgc ttgccaaaag aaaagtcatt gtgtcggaga ccttactggc
    12481 tgtttcggca atatctgttt gagagcggac taaaaattct tcgctggtag ttcctctatg
    12541 gcggctttgt aacagatcaa gaatatcctg ttgcacttta gcaattttat ctttggagac
    12601 cgcagaaaca atgatcatca tatttctcca ccttctaccc agcaaacggg tatagacagt
    12661 tgtgtaaggt gcccaaacac tatcatcctg atctgcaccc atcacatttt gtcccttgga
    12721 ttccataacg cctttaacgg tgaaaggaat attccgaatg cggattattt gtcccacagg
    12781 atctatatct ccaaacaggt tttcggcaac tgttttgcct attacacaaa ccttagctcc
    12841 gttttccaca tcgctgctat aaaacaattc tccttgttca ggattcatat tccggatata
    12901 aaaataatcg gtatctacac ccataacgcc ggttctccag ttttgcgatt caaattttag
    12961 ctgagcaaag ctgttataaa cgggtgatac atacaaagca tcttcaacct gagaacgaat
    13021 agcaaaaaca tcatcaagtt caagtaaact tccacttccg gctgcctgac gcacatttgt
    13081 ggaactacgc gtaaaattta cagtaaccat cagcacattg gttcccatag aagagatttg
    13141 atcttctaca atctttttgg caccttgtcc gatagccagc attgttataa ccgctgctac
    13201 gccgataatt attcccagca ttgttaaaaa agttctggtt ttattgcggg caagcgattt
    13261 gaaagcgatg cgaataattc ctactatact cattcttcat ctccgggaag tttaggcaaa
    13321 tttgccaaat cataagcagc atcgtgagga ttgctattgg gcacatcggc aattatgcgc
    13381 ccgtcacgaa aagttatcag acggtgagca tacattgcaa tatcgtgttc atgggtaacc
    13441 agcaaaatgg tttttccgtt tttatgcaat tcctgaaaga tagtcatcac ttccaaactg
    13501 gttctggtat ccagatttcc ggtcggttca tcggcaagta gaaaaacggg atcatttaca
    13561 atagcgcggg caatggcaac tctttgttgt tgtcctccgg aaagctgatt gggataatgt
    13621 tttgctctat cctctatatt cacggttgaa agggcttcca ttgctttttg taatcgctgg
    13681 tgatgagtca ttttatttct atatagcaga gggagttcca cattttccaa agcggttgtg
    13741 cgcgggagca aataaaagtt ttgaaataca taaccaatca tctgattacg aattttggta
    13801 agttcggcgg gagttatttc gtgaacaccg atatcattga tgtaatattc accgcttgtt
    13861 ggcaggtcaa gacaaccgag aagattcatt aaggttgttt ttccgctgcc actggcaccc
    13921 ataatagcga caaattcacc ttctttaatt tggagggaaa cacctctgag ggcgtgcacc
    13981 tgaacttctc ccatctgata aattttggtt aaattctctg ttctaattag caaattgggc
    14041 attttagaag tgcctcattc caggaccacc ttgagttccg ttattagttt cagagccact
    14101 tttagtattt actccggtga taattgtttc gttacctgta agtccttcta taacttcaat
    14161 atatccgctt tcggaaattc cggttctaat tcctttgggt ttgggttgat tatcttccag
    14221 cacccaaata agtgccgctt tacctgaaga actaattgtt ttagaactaa attcttgttt
    14281 tgttttaccg tatttctttt ggttgaaatt agtgctatca gtttttgcgg gtatagtttt
    14341 tgctttagca agagaagcat aagcttcttt ttgggctttt agtaaaaggt cgtcgctcca
    14401 tttataacca agttgttccc aaatttcttt actgggcttg aaacgggtag ctgtttccga
    14461 aatacgcatc acattttccc gggcttgaat aatgatagtt acattggtag tcataccagg
    14521 aaggagtttt tgttctggat tggcggcatc aataatcaca ctataggaga ccacattgga
    14581 ttccgttgta ggattaagcc gaatttgttt cacattaccg gaaaagtttt cgctgggata
    14641 agcatcaaca ctaaattcaa caggcaagcc aacctttatt tttccgatat ctgcctcgtc
    14701 cacattagca gatatttgca tctgatcaag gttattggct attttgaata aagtgggaga
    14761 actcatactg gctgcaactg tttgtccttc atcaacatcg cgggaaacca caattccatt
    14821 tataggactg gtgatatagg cattggcaag atttttttga gcgcgctgta atgtgagctg
    14881 agcattgtta aagttttgtt gagctgtttg ataagcaaag agtgcttttt tggcatcata
    14941 ttcaggagtc atcttttttt ccagcaattc tgcctgcaga tcgtaatcca attttgcctg
    15001 gtctaaagcg gtttttgcct ttgccagatc agcttttgcg gcttccagat tggtagcaag
    15061 aatttccgta tccagttttg ccagcaattc cccctttttc actttatcat tgaagtcctt
    15121 atagagttta tctatttttc cgctaacttc ggtgcccacg ctaactaaaa cataaggatt
    15181 tagggaaccg gtagcggtaa caacttctct gatattgcca aaggagggag atgttgttct
    15241 gaattcgggt ttgtttttcg ctttgcgcaa ggatattatc acgattatgg cgataagaat
    15301 aagcgcaatt atgatgatat acttaaggta ttttttcatt ggtgttcctt atcaattaaa
    15361 tatatagaag ttaccttacc attttcctaa aacgggttcg gaaagaagat aatttatttc
    15421 ttcctgtttg gcgataatag aatataggtt tgtattataa gagatgtctg tttcaatata
    15481 gttggtgcgg gttttatcca gttccagaag ttcaataaga cctaagcgat aacgttcttc
    15541 tgcctgggta atttgttccc gggattgagc cagttgttcg ctgtaaagtt cattcattct
    15601 ttttaaatat tgcaattctc ttaaagcact gtcgtaatcc cgctgcaatt gatctttctt
    15661 ttcttcaatt gccacttggg aaagttgcct gctgatttta gttctggttc tgctttcggc
    15721 attttcccca atattgaaga ggggatagct taaagttaaa gatatgccgt gattggtgtt
    15781 ataacggtca aaatcaaaat cggcaccgct gacggaacgg tcaaaattgt aagccagatt
    15841 gaggcgggga aaatcatcca gtttgttttg ttttaattgc agattcaggc gtttcagttc
    15901 ctgattgagg atattgagat ccgcattttt ttccgtattg aacgcaggaa tttctttttg
    15961 gatattcagc tccagctctg ttaaaggata attttcatcc tgcatttgga cgagagcaaa
    16021 aagttccttg cgggcattgg caattgtatt atccagtttc attaaagaaa tattggaatt
    16081 taagaccgca atttcattct gctttacctc aaagggagtg gttttgccca attgcaaaag
    16141 tattttactc tgttcccaaa ccctaatttg aatctgtaaa ttttcttcca gagaacttct
    16201 ttgtttttgt gaactgagca cttttaaata ggcactgaag acctcataag cataagctct
    16261 gcgggtctgt tccagtttca tatttgccgt tttggtatcc aaagaggcat acaggtaatt
    16321 gaagtaggca gcatcgttca aactaatggt tttgcctata gataaaccgg cggaactggt
    16381 tacatcatca gtgttgacag cggggtcaaa gtcctttttt attccgccac ttacagaagc
    16441 atctggtaat aaattccatt tggatgtatt cagggttgat tttgagctct gataacttag
    16501 agcgctcttt tgcacactga aactattctc caagccataa gttataagtt cttccaaagt
    16561 ataactttga gctgccagaa gtaatggcaa cagcattaat ataactgtga taactttcat
    16621 ttttcctcta tttcgctttg ttctttcctt gtcctcgtct gtgtcttttt aaatggttca
    16681 tttatttata cctttttgag cggtatatcc tgcaactttt tttcttcact gggcacagac
    16741 cttttaatta cttctgtttc ttagttgcct ttggcgtcaa tcattttttg caccgtaaga
    16801 ttgatatttc ctttaacctc aaataataaa ataaattgaa atggaaatgt taagcggaga
    16861 gagataagta gatttttatt aaacgacgag gacatcattt ttccgttaga ttaaagcagt
    16921 cattctttca tagccattta caggtggaag aatgcctgct tcaacggcgg atttaatgct
    16981 tttgcagggt agattatcag caaagatttg ttggcgggtg agggttttca ggtctatttc
    17041 cggattcttt ttagccatat ttgccattaa gcgatgatac aaatttaccc aagagcgcaa
    17101 aggtttatga ctatttgccc gtaatccatt gtaacccatt aaataaagtc gcaaatcatc
    17161 cttgtattct tccgagggtt ttaaggaaaa tatgttgtga gaaatattgc caattctggc
    17221 gttttcttcc gttaaacggg gataaactct tttcctggca taaacaatac cggatttccg
    17281 gtcaaaacaa aataccagtc catcagcttt accggtgtag ccagccagtc cgtattgaaa
    17341 attaactttc atttttgtta ctcctttttt tgtccttcac ttatataggg tgtgacaaat
    17401 tgaagttttg gtgacaaatc ttaggcataa tattggctgc caaaaaatat agtaaccgca
    17461 actaatcaaa tattgattat agaagttgtg ccagcgatat gattcccata tcattccctt
    17521 atcgcgatat gggaagcata agggaatctc ttgtctggta tatctcctaa tatagtgtct
    17581 gccaaaaagt tatgttattt atattcttgt tttgaaggag gataaaaaag tgccaagata
    17641 tagtttttca gcagccaaaa agagaaactt attgacagtc gggttgtatt tttaaaaagg
    17701 ggatacaatc tgaatatgtt tgttattggt tttataaatc tgcctgctgt tagggaaatc
    17761 cgagaaaaaa attaattaaa tgcataaata actaaaagga gaccaaaatg tctttcaatc
    17821 tgaaaaatcg taactttcta accctgatgg attttactcc cagagaaatt caattcctgc
    17881 ttgatcttgc tatccagctt aaaaaagcca aatatgccgg aacggaacag cagaaattaa
    17941 aaggaaaaaa catcgcttta atttttgaaa aagacagcac tcgcacccgt tgtgcctttg
    18001 aagttgcagc tttagaccaa ggtgcacata caacttattt gggacctacc ggttcacaat
    18061 tgggcaaaaa agaatccgtt gccgatacag caagggtttt aggaagaatg tatgatggca
    18121 ttgaataccg cggatttgga caggaaattg tggaagaact ggcaaaatat gccggtgttc
    18181 cggtttggaa cggtttaacg gatcaggatc atcccactca agtattagct gattttttaa
    18241 ctgctctgga acatttgaat aaacccttaa acgaaatggt ttttgtttat tgcggagatg
    18301 gacgtaataa tatggcaaat gctttaatga taggtgcttg taaaaccgga atggatttcc
    18361 ggattgtaag tccgaaaacg ctttttcccg aacaaaatct gatgcacaaa tgcctgaaag
    18421 ttgcccaaga aaccggtgcc aaaattaccc ttacagacaa tattgatgcc ggggttaaag
    18481 atgcagatgt cctttatacc gatgtttggg tctctatggg tgaaccggat agcgtttggg
    18541 aacaaagaat taacctgcta aaaccctatc aggtaaacgg tgaaatgctt aaaaaaacag
    18601 gaaaaagcac tactatcttt atgcactgcc tgccttcttt tcacgatttg aagaccagaa
    18661 taggcaaaga tatatacgaa aaattcggtt tgactgctat ggaagttacc gatgaggtct
    18721 ttgaaggacc ccattctgtt gtttttgatg aagccgaaaa caggatgcat actatcaaag
    18781 ctgtgatggt tgctacttta ggttaaaaaa ccgaaacact tattaagtca ggagttattt
    18841 acgccaaact aacttcgttt aagaaaaata tctttccggg cgtatatgac tcctgatgac
    18901 cagtagtgct aactatcaca gattatacgg ctaacacgga ttgataatta tagtcatttc
    18961 ttaaagcttc tcctaaaact ataagttttg tcctaatttc cttgttctga gcggttacaa
    19021 ttcttcacca ccaaataaat tctcaagttg tgtaaaagtt atatcggtgg tgacgacttc
    19081 tgacatacag tttatttttt cttcttgact tgttttggga aaatgtgtaa aaggaattat
    19141 attataagtg aggaggtaaa gtgactttat gaacaataat gaatatgaat tataccttgg
    19201 tgatgctctg gaaattctgc aaacaataga aaaagagtca attgatttaa tagtaacatc
    19261 tcctccttat gcagatagta gaactaatac ctacgggggc ataaaaccgg atgaatataa
    19321 tgaatggttt cttcccataa cacaagaact tttaagaatt ctaaaaccca ctggaacatt
    19381 cattttgaat atcaaagaaa aagtagttaa cggagaaaga catacctatg tgatagaatt
    19441 gattttgaat atgagaaaac aaggatggct ttggacagaa gaatttattt ggcataaaaa
    19501 gaattgctat ccgggaaaat ggcctaacag atttcgggat gcatgggaaa gattattaca
    19561 attcaataaa aacaaattct ttaagatgta tcaggaagaa gtaatgattc ctataggtga
    19621 ctgggcagaa aaacgacttt ctaacttaag ccatacggac aaaattagag atacttctaa
    19681 agtaggcagt ggttttggta aaaatgtatc caactgggtt ggtaaagata aggtctatcc
    19741 tacaaatgtt ttacacttgg ctactgaatg cggtaataaa aatcatagcg gaacttttcc
    19801 ttatgctttg ccggaatggt ttattaaact atttaccaga ccgggtgatg tggtcttaga
    19861 cccttttatg ggttcgggaa ctgctatttt tgctgctttg aatatgggga gaaaagcaat
    19921 aggaatagat ataaatcctg aatattataa cttagtttta gggaaaattc aaaatcagca
    19981 acaagtattg ttttaagcga ggttattcgt gattgatgaa aatgacttga atgaatatgt
    20041 agaaaagcat attgagattt ttcacaacaa caggattatg aatatcagca atttgaaact
    20101 gacaaatata ttaaaacata aaaatccgta tctgtttaga gcaaagaata gccctatgtt
    20161 tcttctcaat atcggacatc tctatattac caccggacca catatggaca gtaaagacag
    20221 attttatgtt gtcctgtata aggaacaagg tggaatacct tccgaaaacc ttgataaatt
    20281 caaaacagat cttaagaata gagatgtgtt ttgggggaaa ggagttggta ttgacgacct
    20341 agaattccta gaactggtac aataaaagtt aatcgtttgt aatgttaaac ttaatagcta
    20401 atgagaatca tgcagttaac tgatttagat aattgaaact acctatataa gaggcataaa
    20461 tgaatcagct taaaccctta gaacatatga aagcagaccc caaggatgct atttgtaaga
    20521 accctcccaa tggagatgat tttcgtaata tattccagcg agatcgggat cgaatcatgt
    20581 acagtaaagc ttttcggaga ctagcaggta aaactcaagt cttcttcgct gggcatgatg
    20641 accatctaag aaccaggctt acccatactc ttgaagtatc ccagatagca aggacgatat
    20701 catctaaact gggtcttgat ctcgatctta cagaagcaat agcactgggt catgatattg
    20761 gccatgctcc gtttggtcat gttggagaga gagtgcttaa ctacttttcg aacggctgtg
    20821 agctttataa gggattcaac tatagagaaa aaactgacag catagttgat aatggtgtag
    20881 agactctacc aattgagttt agggggtata aacataattg gcaatctgtt cgtatcgctg
    20941 taaatctaac cagacataga ggtgagttag gtctcgatct atgtaggaat acaatatttg
    21001 gaattctata tcatacagca ctttcttacg gaaagtgccc acacagaagt gcaggatatt
    21061 gtaagcttag agaaagaagc aaggagcgga aatgtggaga tccaatcctt catcttgatt
    21121 actatacgaa caattatggt gagtatcaag atttaagata ttattctgtt gagtcattag
    21181 ttgttgcact ttcagatgag attgctcaga ggcaccatga tattgaggat ggaattagag
    21241 caaatctgat atcagaagaa gaattgaaat taaagcttaa ggattttatc aagttagatg
    21301 tgcctgaaat acatgaaaaa aaacatgtct gctcaaacga aaaggaggat tttataacta
    21361 ttttatcagg actagttgtt cattcctatg tcaaaaaaac tattaactac ttaactgagc
    21421 agttaggaga acgtaaatat aaaaacatta gagagttgat acaaagcact gatttagata
    21481 ctgtgatgaa aaacaaagat atatacaaag tggtagaagg agatgaacaa cttcacaggt
    21541 ttctgaaaga aaggatgtta tcatctcata tagcacaagt tatggatggc cgggcagact
    21601 ttctcattag aagattgttc aaagcctata tatcaaaccc ccagcagctt ccagacaata
    21661 ccatccggat tatgatgaaa acatatagag actaccgaca gagtaagggg tatggaaatg
    21721 ttgcatacag caatgttgat tttgatgaaa ttccaatagg agaaataaga aagcatctag
    21781 ctaaacttca gacaaggggt cgattcagtt ttagagctat tatgcttaga actatttgcg
    21841 attacatatc aggtatgacc gatagaagag ctatttctga atatcagaac ctttatggta
    21901 ttcacaattg ataatggata aggtgaatga atggtacaaa aaagcatcaa cctaacgggt
    21961 gagcagaaga aagttctttt tcttcctgcg accaacccaa ttcaaatcaa gggtgtagct
    22021 ggcagtggta aaaccaccat agctatgtat cgtgctaagt accttttgga tacttacagc
    22081 gacctgttcc gtgaggctga aatcatgatc ttcacataca ataacagcct cacccagtat
    22141 atcaagagct tgcttcccta tgtgatcgga ggttatcagg aaaactccga tgccagaaat
    22201 ccctcaacgc aaggtctacg agtaaaggtt tccacctttc acaagtggtc ttatggtttt
    22261 cttagtcgca atggtgtgtt caatgactac acccagatgg aacgatctga gcataatgaa
    22321 atcctgtgcc acgctataaa ggaaacccgt aaatcgttcc agggggcgag aatattgaac
    22381 aagcccctca atttctttga tgaagagttg tcctggatca agggacagct cttcaagaca
    22441 ttaaacgatt acctgtccac tgccaggatc ggacacggca ccaaagacag ggttgataaa
    22501 aatgataaag cagtgatctg gaatgtttac gagacatact gtttggaaca gatatcccgc
    22561 aagaagatcc attttgatga ccatgcctca tattgcctca accttattga aaagcacaac
    22621 atccaaccag aatacaccca catcgtagtt gatgaagccc aggacatgac taaagcccag
    22681 ataatggtga tcaaatcact cgtcaagcca tccacaaaca gccttactat tattgccgac
    22741 actgcacaga gaatatacaa aagtggattc acctggacgg aagtgggtat taatgtgaga
    22801 ggtgggagaa ccgtcgagct caagaaaaac taccgaaaca ccaaggaaat tgctctggca
    22861 gccaattcct tgatgtccca tgatttggag gatgatgatc tcacggctat ggacatagac
    22921 agcattacca gtcacggcga taaacctgta ttgatctatt atagtgatgc cgaaggggca
    22981 aatagagcat tgttggagaa gaccaaggag gcaaagctca agggaggatc aattgttgta
    23041 ctacatcgct ataggaatat gctggactat tattcgaagc tgttgaaaaa ttgtggtatt
    23101 gccactgagg taatcagtcc agacaatcct gcagattatg ctagcaactc agttaaaata
    23161 tgcaccttgt catcggtaaa ggggctagag tttgaccatg tgattatcat ggagctctca
    23221 agcatgatta ttccctcccc tgatggcttt ggtcaggata atgacgagta tcatatcacc
    23281 accgaaagaa agctgcttta cactgctatg acaagggcta aacagtcatt attcctgttt
    23341 gtgccccaag cttatcccag catgttcatt gatgagatag acaaacaatt tcttactgta
    23401 attcgttagg tataagcttg tttacggaaa aaccagactc aggtcaattc aaacaagaga
    23461 ttaaacgcag ggggatcact cggctgatcc atttcactac tactatcaat ctcctgggaa
    23521 tatatgagtc tggatttctc tactccagaa aagaactaga agatcttgat atcaatatga
    23581 ccgatatctt ggattatatt caattcaatg atcagataag gtatgatgac aagacctata
    23641 tcaatctatc cattgaaagg ccgaactcgt acctattcaa gcgtttcatc gacaacacaa
    23701 gagataaacc ttatatttcc tggtgcgtgc tgagcattga ccccaagtac atttatgaac
    23761 aggggacact cttttcaatt actaatgcgt caaatagtta caataaaaaa aatgtaggca
    23821 taactggaga ttttcacaag ttccagatga tgttcgacga tagtttgcct attgtctctt
    23881 caagaagaac catactcgtg tatagaacag gactccccga tgcatataca acccatgagc
    23941 aggctgaagt gctggtgaaa atacctatcc ccgtagctga tataattagt gtttcatttc
    24001 ctacccaaga aaagcttgca g
//
LOCUS       DAOPFL010000018        23778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2202_length_23778_cov_95.194368, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000018 DAOPFL010000000
VERSION     DAOPFL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 23778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23778
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2202_length_23778_cov_95.194368"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..978)
                     /locus_tag="PLG69_02540"
     CDS             complement(97..978)
                     /locus_tag="PLG69_02540"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPI42420.1"
                     /translation="MYNEISQLPLVQQSRQGKVRDIYDLGETLLIVSTDRISAFDVVF
                     PTLIPDKGKILNAISVYFFNTTKDIVPNHFITADISEYPVELQRFGEYLEERSMLVKK
                     TRVIPFECIVRGYLSGSAWKEYKKSNTIGGMLIAEEMQESQKFPNPLFTPSTKAEKGH
                     DENISYREFMSRMDESLAVHIKDKSLELYSYAHDLLLKKGIILADTKFEFGTIGSEVL
                     LIDELFTPDSSRLWSLEDYEMGKSPKSYDKQFIRDYLENSGWDKQSPPPPLPEEVIEQ
                     TRQKYLTILKIITGKEL"
     gene            complement(988..1926)
                     /locus_tag="PLG69_02545"
     CDS             complement(988..1926)
                     /locus_tag="PLG69_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42421.1"
                     /translation="MNEIKIDKNIQNEFDALPKGWVFYHIFGAGEVLYAGYSANLKAK
                     LKFFSNKAKEGGIYQELWNLAEKLSWVTYPQAIFALIHYKCYLTENSPLYQYQIRPWA
                     DYVYLALDSHRFPFITIQEHTNDDWFYLGPFRSRFFLIDVFDIYARILKLPACETGEY
                     PCEKREKNLCRGWCLALDETSEKPTDYDLDKLESLLKEAFLVPENNILELIQQERNKY
                     FNELEFDKSSLLDEDIEILNKYREWLKFLISAKHLEYESNELKIQKGKITYCRFQGKD
                     YYFPVDNIEFRINETLALNLNSVDESKVIYNFLQKM"
     gene            complement(2597..3397)
                     /locus_tag="PLG69_02550"
     CDS             complement(2597..3397)
                     /locus_tag="PLG69_02550"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI42422.1"
                     /translation="MKRLSLGKFFKGMNLTVFTLVMVFLYIPILILIIFSFNDAKIIT
                     GWKGFTFKYYVLLWQNKTILEAFRNTMLIAGLSTLISTVFGVLGALGLEYHKFSGRKL
                     FSALIYIPLVIPDILMGVSLALLFNFTRVNTGMLTVLIAHITFCISYTVIVIQSRLEG
                     FDYSLVEAAMDLGAKPATIFWKIKLPLIMPGIIAAALLAFTLSIDDFIITFFTSGRGF
                     DTLPIYVEGAIRRGTITTINSLSTIMIGLTLMLVIATKRIRKIVISSL"
     gene            complement(3394..4299)
                     /locus_tag="PLG69_02555"
     CDS             complement(3394..4299)
                     /locus_tag="PLG69_02555"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI42423.1"
                     /translation="MKIIPSDYPNSALQIAAERRKNFTSWILTAPALLWLIFFFLVPY
                     IIVIIYSFLTADIYDVKFEFTLDAYRQSFTPAYLHTFYLSFRLALLTTVLCLLLGFPV
                     AYYIARAKDKVKNTLLIFIIIPFWTNLIIRIFSWRIFLAYNGVLNNLLLSLGLISQPL
                     EIMRTDLAVILVMVYVYLPYMILPLYSVLEKLDWSLLNASMDLGANEVKSFFRITLPL
                     SKEGIFAGGLLVFIPALGSYLIPQLVGNQKSLYLGQVITYKVKNIPRNWPMASALSLT
                     LLLIVALFLLVIYFLYRKSKSRRQV"
     gene            complement(4296..5483)
                     /locus_tag="PLG69_02560"
     CDS             complement(4296..5483)
                     /locus_tag="PLG69_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42424.1"
                     /translation="MEEIRLENLSKDFDGFVAVENVSLAIQSGELFSFLGPSGCGKTT
                     LLRMIAGFEIPSTGRILIGNTDITDLPPYKRQVNTIFQNYSLFPHMTVFDNVAFGLRI
                     RKSPKNEIKNRVREMLSLVKMEDQANKFPEQISGGQKQRVAIARALINQPKVLLLDEP
                     LAALDLKLRQHMLIELMNIHDAVGITFIYVTHDQSEAMSISDRVAVMNYGNIIQEGPP
                     DDIYERPNSVFSAYFIGETNLISGEVISIEDDYVQIRCPDGTGNSFEIDSTFHFQPNI
                     GQELTVSLRPEKIAISRNKPRYQDDINILRGVIEDILYLGSHTQYIVRVGALKIRVFS
                     QHKRVYFDDTALDWEEPVWLFWHDTDSWLINEIPENVLTPEEISEGGVNHYQRSIKKP
                     AVQ"
     gene            complement(5734..7752)
                     /locus_tag="PLG69_02565"
     CDS             complement(5734..7752)
                     /locus_tag="PLG69_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPI42425.1"
                     /translation="MKKQRILRILLLCSVLCILSSTAFAHHNLPADKANAEIRFTETN
                     PPVAPVRPIAEFEPASDVLIRYPLGIPVSLVVQLANTANVICIVSSSQQSSAISTFTN
                     AGVNMERVSFLNAATDSYWTRDYGPWFIFDGNGEYGMVDFIYNRPRPNDNLIPQVFAN
                     HFALNYYGMNLQQTGGNYMSDGINTAAQTTLVYTENGNNQTNVNTKMQQYLGITNYLV
                     MADPNNTYIEHIDCWAKFLAPDKVMIRSVPASHSQYNAIENAANYFATHNCAWGYPYR
                     VYRVYTPSNEPYTNSLILNKKVFVPIVGSSNDNSALQAYREAMPGYEVIGVSQTSSAP
                     WESTDALHCRTHEIPDKNMLNIVHTPWHSIVPVGTDIIINTEIIAHSGQPLYTDSLFV
                     CYKVNSGAWQRNYLQPLTRNYYTVTLNGFAYGDTIRYFIHAADQSGRSVDHPVFAALD
                     PHLFVIQPDLTPPVLSHNPLSSISNQSEPISIIVSANDESGISQLLFRYKIDDSEVFA
                     YPMEPLTEDSYIFQYYPEFTTNNHTFYYSFMAYDGANPPNISFLPGQNLWYEVPILIV
                     SVNDEIQTPNLQEGIMGVYPNPFKAKGIDAIRLKYFSPQNIPFTFKVYNLKGQLIYTQ
                     TFFPEGKVIQEIKWNGRDNKGNLSPNGMYLIEILQGSKNHKSKLIIVK"
     gene            complement(7910..8602)
                     /locus_tag="PLG69_02570"
     CDS             complement(7910..8602)
                     /locus_tag="PLG69_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42426.1"
                     /translation="MPSHLYIIIGAYGSGKSEYSIHLARRFKTQGLDVVLADLDVVNP
                     YFRSREMQDDFIQEGIDIIAPEGEYKHSDLPMISPRVKGAIQNKAKTVILDVGGDPAG
                     CRTLGRFEEPIKTRGYSMLYIVNTMRPFTSTPEEIISMKEALEATSRLKITEIICNTN
                     LMDETTVEIVLKGIKIVEKAAELSGLPFKTYLVLNKYEKIVPDNLGGKKREVMAYNLK
                     KPWELFVAKGIC"
     gene            complement(8677..9345)
                     /locus_tag="PLG69_02575"
     CDS             complement(8677..9345)
                     /locus_tag="PLG69_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPI42427.1"
                     /translation="MNNFQAVIFDMDGTLIDSMQLWRNVDREFLHTRGLIVPPDLFSE
                     LPQGNSFIQTAQYFKDRFGLPDSPESIMQEWTKMVSKHYETDIMLKPGVVELLSCLQE
                     KGIKIGLGTSNSLELAKKVLIRNSVWQYFQCAVTGDINLKGKPYPDIYLLAAERLQEK
                     PENCLVIEDTLTGVQAGKSAGMTVFAIYDEDSSDQHSLIKEIVDGFYWDFKSLAEDLF
                     RTWK"
     gene            complement(9386..9461)
                     /locus_tag="PLG69_02580"
     tRNA            complement(9386..9461)
                     /locus_tag="PLG69_02580"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9426..9428),aa:Gly,seq:gcc)
     gene            complement(9463..9550)
                     /locus_tag="PLG69_02585"
     tRNA            complement(9463..9550)
                     /locus_tag="PLG69_02585"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9514..9516),aa:Leu,seq:taa)
     gene            complement(9563..9636)
                     /locus_tag="PLG69_02590"
     tRNA            complement(9563..9636)
                     /locus_tag="PLG69_02590"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9602..9604),aa:Cys,seq:gca)
     gene            complement(9649..9724)
                     /locus_tag="PLG69_02595"
     tRNA            complement(9649..9724)
                     /locus_tag="PLG69_02595"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9689..9691),aa:Gly,seq:gcc)
     gene            complement(9777..10322)
                     /gene="amrA"
                     /locus_tag="PLG69_02600"
     CDS             complement(9777..10322)
                     /gene="amrA"
                     /locus_tag="PLG69_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPI42428.1"
                     /translation="MLTLEQKKELLSLARNAISARFKHQSVSLPEDPAFSPKRGVFVS
                     LHIGDNLRGCIGYIKGYKSIAESVKEMAEAAAFQDPRFPPLTEKELEKVTIEISILSE
                     LIPMQKNELPVIGKDGLYIQHPYGSGLLLPQVAVEYNWQPETFLREVCRKAGLPPNAY
                     LDPASVVYRFTADVFSEADTF"
     gene            complement(10341..11159)
                     /gene="amrB"
                     /locus_tag="PLG69_02605"
     CDS             complement(10341..11159)
                     /gene="amrB"
                     /locus_tag="PLG69_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPI42429.1"
                     /translation="MIRNPMHAGTFYPRFEQQIKRQIEGWISNAVTPLSSERALGVIL
                     PHAGYMYSGECATLGLHSISHENIDSFIILHPSHQANFFDFSVSPYQEYVNPLGTLDL
                     DMELYNKIAPEADQNIPLILHQEEHSMEIQLPILNYFFPKAKILPIMFGNQIPAVAKR
                     LADILYETIYSSTKRIVILCSSDLSHYHRARTAEEMDGILVKDVTALDPEHLWQDIIH
                     SDCEACGIGGILCLLYYAQHYTNAKMKVIQYTHSGKISGNDSQVVGYLSAKLYI"
     gene            complement(11156..12028)
                     /locus_tag="PLG69_02610"
     CDS             complement(11156..12028)
                     /locus_tag="PLG69_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HPI42430.1"
                     /translation="MKYIIVIMIIIMNLTLLNADEYRIVDSRNSKTLTLQQMAKGLEK
                     YDIIFFGEFHDNATLHQLERELVPLLDTKRELILSLEMFERDVQSDLDAYIEGWLTED
                     EFLAKSRPWSNYQDDYRPLIEYAKQKKLTVIAANIPRSIAGKMARTGPDFTETLAEED
                     KKWLPDKIDYPDDGYKKAFLATLEDMHSPMMNNNPDWLYQAQCLKDETMAESIVNALK
                     MKPKARVLHFNGDFHSRNFSGTVSRVQSILPNKKIAVISPSYSENWQTANLTTEEKKS
                     GTYIIYLPLQGGEQ"
     gene            complement(12048..12641)
                     /locus_tag="PLG69_02615"
     CDS             complement(12048..12641)
                     /locus_tag="PLG69_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPI42431.1"
                     /translation="MLYSIEKKTDSEIKIKRSRFVAVLYPLPYVEEVKDILNEHYRLF
                     SDATHNCYAYIHGLNREIIYYSDAGEPTGTAGKPILNTLLRNDLTNILAVVSRYFGGI
                     KLGVKGLIAAYSEAVEQAILKSNLIEAKLLISFPLVCDYASFECLKHSFLAIGGEIKQ
                     VIWSEEVSFILNLPAEQEKEFRKIIDGNKHKIRLNYQ"
     gene            complement(12697..13986)
                     /locus_tag="PLG69_02620"
     CDS             complement(12697..13986)
                     /locus_tag="PLG69_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HPI42432.1"
                     /translation="MNTTGLKNDNIVALVDCNNFYVSCERVFNPKLNNKPVAVLSNND
                     GCIVSRSQEIKDLKIPMGAPGFKYEGLIKKHGGTLLSSNYALYADMSSRVMEVLSLFS
                     PEVEIYSIDEAFLGLNGFKNRDLENFGKKIKRTVFQWTGIPVSVGISQTKTLAKVANH
                     FAKRYSAFQGSLCLLDDERIAKALAKTPVSEVWGIGRQYDKFLRQHKIETALQLRDAE
                     DKFIEHYLTSMGLKTVLELRGYACIDLDEAPANKKSIVTSRSFGKQVSSLNDLQEAIS
                     EYVTRAAEKLRKQKSVAGLLMVFLSTNRFKEGPQYNNSLSTTLFPPTAYTPELIKTAL
                     NLLDDLYLPGFEFKKAGVMLADIIPEKDVPLSFMDTNYLDDKRKKLMEAMDKLNRTYG
                     QDTLFYASSGIKKDWKMKRAKLSPHYTTHWNDLPKVK"
     gene            14982..15173
                     /locus_tag="PLG69_02625"
     CDS             14982..15173
                     /locus_tag="PLG69_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42433.1"
                     /translation="MVIIIRHKTRNFIRGYKFYCSNVVPAKAGIQGIIKITYASFSPG
                     SKPVVSICRSYGTLCRKKR"
     gene            complement(15510..16694)
                     /gene="ftsZ"
                     /locus_tag="PLG69_02630"
     CDS             complement(15510..16694)
                     /gene="ftsZ"
                     /locus_tag="PLG69_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPI42434.1"
                     /translation="MIEFDEKPAQIGTNIKIIGVGGAGGNALNTMIENNLFGVEFIAA
                     NTDIRDLTKSKANMKLQLGKKLTRGLGTGANPELGARSAEESKEDIKNHLDGADMVFI
                     AAGMGGGTGTGASPIIAKIAREMGILTFGIVTSPFPYEGKKRAENAIYGIKHLREFVD
                     TLLIIPNEKLCEIYANLTLKEAFKKAEFVLYEAARAVSDIINVTGLINVDFADVKAIM
                     QNMGYALIGSGIAEGENRAINAARAAIDNPLLSHISLQGCQSLLLNITAGYDILMSEF
                     DEVSNVIVSETGKAANIIMGIILDDAMAGKIQVTIIATGLEKEDKEHTIDFPGLPDFG
                     EKSVPSITQSEDTNAEIEDIFARLSINQSTPKEEVKPAANEPTRIPPLKTDVPSFLKA
                     LD"
     gene            complement(16707..17978)
                     /gene="ftsA"
                     /locus_tag="PLG69_02635"
     CDS             complement(16707..17978)
                     /gene="ftsA"
                     /locus_tag="PLG69_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPI42435.1"
                     /translation="MKQSIITALDIGSSNVRCIIAKEVGDGRLEILGLGEYPSEGIEG
                     GIVKDIQALSGCVAKAISTAEKEGKTSANNIYCNITGEHIRTQFGDGRISIPSETPNE
                     PGEITQEHIEQVINDAKNSVKIQKGLERFRILHGIPHNFVIDNQDDIHNPVNMNGFHL
                     IAKVYIILAELTPLRNLSKCIQLAGYEINPENFILNHIAISESVLSEDERRLGALVLD
                     IGGGTCDLSIYNRGSLEKILVLPMAGKNITEDLAIGLKTTLGNAEYIKVEYGNALASS
                     VDQTEEIDVEGISGRSTSRKTKFLVSHVIQHRVEEMLSLCYNKAKEFYTPELVTSGII
                     LCGGTAKLKNIEVVLSEAFNLHVKIATPDLSRLNGMISRLEDPAYATVVGILYYAAGK
                     NNEPVSKGFTLPNIKGKKIVDKVKKILKDFT"
     gene            complement(17975..18757)
                     /locus_tag="PLG69_02640"
     CDS             complement(17975..18757)
                     /locus_tag="PLG69_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPI42436.1"
                     /translation="MIHKLMELNKGTRKRRGNSRYYLFFFFALIGVALLGTGIWFGLT
                     HIDLFTLQKVSVIGNEAIPDTLIYKITQPYIGMNLLAIPAEDIKNKVMNLSRVKDVKL
                     HKRLPSTIKLEINERKAAIYLKTIEGDLHPIDSEAVVLMKYSPIYKEDMPIYSTYLSN
                     HQIKPGHKLKNAGLQQVLQLHKRITKEAPDFLPQISEYYLIDKTVNIIDAKTGTRIIP
                     AQEDLAKQLARYQFVQENGNINKNSVVDLRYKNQVVVKAGNK"
     gene            complement(18823..20217)
                     /gene="murC"
                     /locus_tag="PLG69_02645"
     CDS             complement(18823..20217)
                     /gene="murC"
                     /locus_tag="PLG69_02645"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPI42437.1"
                     /translation="MLGRTRKIHFVGIGGIGMSGIAEFLHNQGLEITGSDLKKSDITT
                     HLESLGIKIFEGHNPENIGDADVVVKSSAINDDNPEIISANEMKIPVIRRAELLAEIT
                     RMSFSIGIAGTHGKTTSTSMAGSVLESAGLEPTIIVGGKVKNFGSNNVMGSGKYIVVE
                     ADEYDHSFLTLTPCIAGITNVDADHLDCYRNLDEIKSSFIEYANKVPFFGSVIACLDD
                     PGVQAILPFIHKKIVTYGFSRQADVQARDIDMQGFNSSYDLLFKDYKLGRISLKVTGK
                     HNIQNSLLAASIGLELDIPFPAIQDGLGKFSGVYRRFDLKGEAGGITIYDDYAHHPTE
                     IIATLEGFKDSAKRRIVALFQPHLYSRTRDFYEEFGKSFFSCDCLILAPIYPAREQPI
                     PGISSKLIADAAIQSGHHNVHLINSNSEIVSKTLALLKEGDILITMGAGNIWQYGEEI
                     LRQLKNYVDTNHKQ"
     gene            complement(20211..21299)
                     /gene="murG"
                     /locus_tag="PLG69_02650"
     CDS             complement(20211..21299)
                     /gene="murG"
                     /locus_tag="PLG69_02650"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPI42438.1"
                     /translation="MKFIFGAGGTGGHITPALALADELVRYKHNVLFLGNRQSIEETL
                     CASAGYPFRQIKVQKLHRSLKPENMLVPFYLLSSVITAVNIMKKEKPQAVICTGGFVA
                     GPVAISAALLKIPLFFHESNSYPGLVTRKMAKWINTIFISFENSRKYLSRAKLVNYGI
                     PLRQITNKDQKPFELSSLGLKNDKPTLIVSGGSQGSVAINTVVNQAISDILSLGYQII
                     WQTGKISYERFSAKHEGREGVYIFAFSPDLPKMLSQCSLAITRAGAMTIAELEENRLP
                     AILIPLPTAAENHQYYNALSQQEKGVAVLLEQSKLTPFSLVENIKKITENLEYYRNKL
                     ASLPPNQAAQKIIEYILNYLNSKEEEEC"
     gene            complement(21296..22435)
                     /locus_tag="PLG69_02655"
     CDS             complement(21296..22435)
                     /locus_tag="PLG69_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPI42439.1"
                     /translation="MRRNKTPSYIVSFDKVILITYCLLCLVGLITLLDISSVQSSMKY
                     FYRQLVFLIISMITVIVILYTFNLEKLRVLSPYFVYLTIILLIIVLLKGSTVKGATRQ
                     LSLGFINFQPSVLARLALVFYFAHILDKKYDELVASNPLHFFTNFFALIVITGITFLL
                     IIMERHLSTLIIGGLTLYAMLIYAGTKKRVLISLALIGIIAGVLILANGADYRKGRLT
                     TYKKFSLFLRPEGEIKIEDSDYQVKESLTALSSGGLIGTGMAKGRAKHYYLPEARTDY
                     VYTVIGEEWGFIGALIVFGLHCFLFFRCFRMANSQENRFLRFLGVGLAMNIFCNVLVN
                     TGVAMSILPPTGNTLPFISYGGSALLIDSIALGMLLNISAQRRYV"
     gene            complement(22422..>23778)
                     /gene="murD"
                     /locus_tag="PLG69_02660"
     CDS             complement(22422..>23778)
                     /gene="murD"
                     /locus_tag="PLG69_02660"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPI42440.1"
                     /translation="RMIDKDKKYGIIGLARSGFASAYKLKELGVTPFLSELRPAKEIP
                     TAEKLEKDFPCEFGGHTNKLLECDCLIVSPGIPLNTPILQKAKEKNIELISEIELGYR
                     IKAKDSKIIAVTGSNGKSTTVSLIHHILTGLGKKSLLAGNIGDAFCSYDIEKPGWNYI
                     VLEISSFQLDLINSFQPDVAVLLNITPDHIDRYNSFEDYALSKVNIFKNQEMTDTAVL
                     FWDSPEVKHFTKDIKSRKLYFSLSPNDNAFAYLANNYIQFGSETKVPLSYFAIKGPHN
                     YANAMSAMLAVYALIPEPTKIAEAVKGFKSLPHRLEYVDTIKGVSFYNDSKATNTDSV
                     RSALNSFEQPIRIIMGGSDKGEDYSVLAADLQKKVKKAYLTGDTQEKMRKTFSGKIPL
                     ECIADFELTVRKAFSDSGEGDIIVLSPACASFDRFNNYEERGNFFKEIVARIGKENEK
                     K"
BASE COUNT         7400 a         4684 c         4229 g         7465 t
ORIGIN      
        1 taattttcca gttcggtctt atagcttcca gttcaatttt caggttttca accaggtctt
       61 cttcgtcatc cacaatgcag ataattttct tcgttttcac aattctttac cggtgataat
      121 tttaagaata gttaaatatt tttgtcgggt ttgctcaatc acttcttccg gtaaaggagg
      181 aggtggtgat tgtttatccc aaccgctgtt ttccaggtaa tcccgaataa attgtttatc
      241 gtaactttta ggacttttgc ccatttcata atcctccaaa gaccaaagtc gggaagaatc
      301 gggggtaaac aattcatcaa tcaggagaac ttccgagccg atagtgccaa attcaaattt
      361 agtatcagct aaaataatgc cttttttcag cagtaaatcg tgagcgtaag aatatagttc
      421 cagggattta tctttgatat gaacagcgag ggattcatcc attcggctca taaattcacg
      481 gtagctgata ttttcatcgt gacctttttc cgcctttgta ctgggggtaa aaaggggatt
      541 tggaaatttt tggctttcct gcatttcttc tgcaatcagc attcctccga tagtattgct
      601 ttttttatat tccttccagg cagaaccgct gaggtagcca cgcacaatac attcaaaggg
      661 aattacgcgt gtttttttta ccagcatact gcgttcttcc aaatattcgc caaagcgctg
      721 taattctaca gggtattcag aaatgtcagc agtaataaaa tggttgggaa caatatcttt
      781 agtggtgtta aaaaaataaa cggaaatagc atttaatatt ttccctttat cgggaataag
      841 agtggggaag accacatcaa atgcggaaat gcggtctgtg ctcacaatca aaagtgtttc
      901 tcccaaatcg tatatatcgc ggactttgcc ttgccggctt tgttgaacaa gaggtagttg
      961 actaatttcg ttatacatta aaactcctta catcttctgt aaaaaattgt agattacttt
     1021 gctttcatct acactattca gattaagagc aagtgtctcg ttaatacgaa actctatgtt
     1081 gtcaactgga aaataatagt ccttgccctg aaatctgcaa taggtgattt tgcctttttg
     1141 tattttcagc tcgttacttt catactccaa atgtttagcg gaaataagaa atttcagcca
     1201 ttcgcggtat ttgtttaaaa tttctatatc ctcatccagc agcgatgatt tgtcaaattc
     1261 cagttcatta aagtatttat ttctttcctg ttggataagt tccaaaatgt tgttttcagg
     1321 aaccagaaaa gcttctttta aaagggattc caatttatcc aggtcataat ccgttggttt
     1381 ttcagaagtt tcatcaagag ccaagcacca acccctgcat aaattttttt ctcttttttc
     1441 acaaggatat tcacctgtct cacaggcagg tagttttaaa atgcgcgcat aaatatcaaa
     1501 tacatcaatt aagaaaaaac gactgcgaaa aggtcctaaa taaaaccagt catcattggt
     1561 atgttcctga attgtaataa acggaaaacg atgtgaatcc agcgctaaat aaacatagtc
     1621 cgcccaagga cgaatctgat attgataaag aggagaattt tctgttaaat aacatttata
     1681 atgaattaag gcgaaaattg cttgaggata tgttacccag ctcaattttt ccgctaaatt
     1741 ccacagttcc tggtaaatac caccctcttt ggctttattg gaaaaaaatt ttagctttgc
     1801 cttcaaattg gctgaataac ctgcgtaaag gacttctcct gcaccgaaaa tatgataaaa
     1861 aacccagccc ttgggcagtg catcaaattc attttgtata ttcttatcta ttttaatttc
     1921 gttcatataa caacatttct tcagttaggg tttatgtcaa acgaaatcct aaataataaa
     1981 gaggatgcta aacttctgga agaacggcgt ccttaccttt cacaagtatt catttttttg
     2041 ttaccgtaat tatatagata aagaaataaa gcgtaagagg aggttaacgg ggatgtatat
     2101 gataggacac tataaccggt aaaaaaagtt gtttaaaata gtaacatact aagagcttac
     2161 tttattcttt cccatcctcc acccatcctt aaggctgggt tgaggatagg ttataattaa
     2221 ggaaggacaa attgcagaaa caacagtaaa aataccagat aaacgatata ataaattctg
     2281 gcaaaaaagg ttaaagatat tgaaggggag attaatatgg aaaagttttg ggttcttggc
     2341 aaagatgtat gagcgaagag ttatttagta ttttttccga ggggtttaca cacccatcgc
     2401 tatcattgtg tcgccctgac gagcttttaa agaatttcaa ccctttagat attaaaatcc
     2461 tttttccgag ctaaattcat tttacatttt acattctgaa ttcttcattc ttcatccttc
     2521 acttctttat ttgacaaatt atcttatttt atactcccga aaattaccat aatcctgttc
     2581 atcctcaatc tcttgtctat aaagaagaaa tgacgatttt ccgaatgcgt tttgtggcga
     2641 ttactaacat taaagttaag ccaatcataa ttgtggaaag ggaattgata gtagtgatag
     2701 ttcctcttct aattgcacct tccacataaa ttggtaaagt gtcaaacccg cgtccggaag
     2761 taaaaaaagt aataataaaa tcatctatgg aaagggtaaa agctaaaagt gctgcagcaa
     2821 taattcccgg cataattaga ggtagtttta ttttccagaa gatagttgcc ggttttgctc
     2881 ccaaatccat tgctgcttcc accagagaat aatcaaatcc ttccaaacga ctttgaataa
     2941 ctataaccgt ataggaaatg caaaaagtaa tatgagcaat caaaacggtc agcattcccg
     3001 tattaaccct ggtaaaatta aataataaag ccagggaaac acccattaaa atatccggaa
     3061 ttacgagagg aatatagatt aaagcagaaa ataatttgcg tccgctgaat ttatgatatt
     3121 ccagtcctaa agcgcctaac acaccgaaaa cggtagaaat aagcgtagaa agtcccgcaa
     3181 tcagcattgt atttctaaat gcttccagga tggttttatt ttgccaaagc aaaacataat
     3241 acttgaaagt aaaccccttc catccggtaa taattttggc atcgttgaaa ctgaagataa
     3301 tcaaaattaa aatgggaata taaagaaaaa ccatcaccaa agtgaaaacg gtcaaattca
     3361 ttcctttaaa aaacttgcct aaagaaaggc gtttcatacc tgtctcctgg atttgctttt
     3421 gcggtaaaga aagtatatta ccaataaaaa caaagctaca atcaataaca gggttaatga
     3481 cagagctgaa gccataggcc agttgcgagg gatatttttt actttatagg tgattacctg
     3541 tcctaaatag agcgattttt gatttcctac caactgagga attaaatatg aacctaaagc
     3601 aggaataaaa acaagcagtc cacccgcaaa aataccctct ttggacaggg gtaaggttat
     3661 gcggaaaaat gatttaactt cattagctcc taaatccatt gaagcattca gcaaagacca
     3721 atccagtttt tccaaaacac tatatagtgg caggatcata tagggtaaat aaacatagac
     3781 catcactaaa ataactgcaa ggtctgttct cataatttcc agaggttggg atattaatcc
     3841 taaacttaaa agaagattat tcagcacccc attgtaagca agaaaaatgc gccaggaaaa
     3901 gatgcgaata atcaggtttg tccagaaagg aatgataata aaaatcagaa gggtattttt
     3961 taccttatcc tttgcccgtg ctatgtaata ggcaactgga aatccaagga gcaaacataa
     4021 taccgttgtt aacaaggcaa gccgaaaaga aagataaaaa gtatgcaaat aagcaggtgt
     4081 aaaactctga cgatacgcat ccaaagtaaa ttcaaacttt acatcataaa tatcggcagt
     4141 gagaaaacta tagatgataa caataatata gggaacaaga aaaaagaaaa ttaaccacaa
     4201 caaagccggc gcagttaaaa tccaggaagt gaaattcttt ctgcgttctg ctgcaatttg
     4261 taaagcggaa ttgggataat cggaaggaat aattttcatt ggactgccgg tttttttata
     4321 gaacgctgat agtgattaac tccgccttcg gagatttctt cgggagttaa tacattttcg
     4381 ggaatttcat tgataagcca gctatcggta tcatgccaga aaagccagac cggctcttcc
     4441 caatcaagtg ctgtatcatc aaaataaact cgtttatgct gactaaaaac cctgattttt
     4501 aaagctccaa ctctaactat gtattgtgtg tgagacccga ggtataaaat atcttctatt
     4561 actccccgta aaatatttat atcgtcctga tagcgtggtt tattacggga aatggcgatt
     4621 ttttcgggac gcagggaaac ggttaattcc tgccctatat ttggttgaaa atgaaaagtg
     4681 ctgtctattt caaaactgtt tcccgtacca tcgggacatc ttatttgcac ataatcatct
     4741 tcaatgctga taacctctcc gctgattaaa ttggtctctc cgataaaata tgccgagaaa
     4801 acgctattag gtcgttcgta aatatcatcc ggaggtcctt cttgaataat attgccataa
     4861 ttcatcactg ctacacggtc tgaaatactc atcgcttcac tctgatcatg agttacataa
     4921 ataaaagtga ttcccacagc atcatgaata ttcatcagtt caatcaacat atgttgacgc
     4981 agttttaggt ctaatgcagc caacggttca tccaaaagca acaccttggg ctggttgatt
     5041 aaagccctgg caatggctac acgctgtttt tggccacccg aaatctgttc cggaaattta
     5101 tttgcctgat cttccatttt aactagcgaa agcatttcgc gaacccggtt tttaatctca
     5161 tttttgggac ttttcctaat tctcaaaccg aaagcaacat tatcaaaaac cgtcatatgc
     5221 ggaaaaagag aataattctg aaaaatagta ttaacctgtc ttttataagg aggtaaatcg
     5281 gttatatcgg tattgccgat taaaattcta cccgtgctgg gaatttcaaa accggcaatc
     5341 attcgtaaaa gagttgtttt tccacaacca ctgggaccca ggaaagagaa taactctcca
     5401 ctttgaatag ctaatgatac attttctacg gcaacaaaac cgtcaaagtc cttacttaag
     5461 ttctccaaac ggatttcttc caactttgtt tatacctcct ttataagctt ttttagataa
     5521 gccgcgggaa attaaaactt ttttccttaa cttctcctcc ttgattatag gcaaaaaaat
     5581 acagtgggga atgccgggcg gcatccccac tgctcggagg gcattctatg tagggtctgt
     5641 aggcttgaat catttataaa tacctacaga atcttgtcaa gaaaaaagtg tagaattttt
     5701 tcattttcta agttgaaata tatcaacata gttctatttt actattatta gcttgctttt
     5761 gtggttttta gagccctgta aaatttctat taaatacatc ccgttgggag ataaatttcc
     5821 tttgttatcg cgtccattcc attttatttc ctgaataact tttccttcag ggaaaaatgt
     5881 ttgagtataa attagctgac ccttcagatt gtaaaccttg aaagtgaaag gtatattttg
     5941 cggtgaaaag tatttcaacc ggatagcatc aatacctttg gctttaaagg gattgggata
     6001 aactcccata atgccttcct gtagatttgg agtttgtatt tcatcgttca cagaaacaat
     6061 taaaattggc acttcatacc ataaattctg tccgggaaga aaacttatat taggaggatt
     6121 ggctccatcg taagccataa aactgtaata aaaagtgtgg ttattagtag taaattcagg
     6181 ataatactga aaaatataac tatcttccgt taacggttcc atcggataag caaaaacttc
     6241 cgaatcatct attttgtaac ggaaaagcaa ctgactgatg ccactctcat cgttggcaga
     6301 aacaattatg cttattggct cgctttgatt actaatagaa gaaagaggat tatgagataa
     6361 aaccggagga gttaaatccg gttgaatcac aaaaagatga gggtctaagg cagcaaatac
     6421 cgggtgatca acgcttcttc ctgattgatc tgctgcgtga ataaaataac gaatagtatc
     6481 tccataagca aatccattta aagtgacagt ataataattg cgagtaagag gttgcagata
     6541 atttctttgc caagcaccgg aatttacctt ataacaaaca aaaagagaat ctgtataaag
     6601 tggttgaccg ctatgtgcaa ttatttccgt attgataata atatccgttc caacagggac
     6661 aatactatgc cagggagtat gaacaatatt cagcatattt ttatcgggaa tttcatgggt
     6721 tctgcaatgt aaggcatcag tagattccca aggtgcagaa ctggtttgag aaacaccaat
     6781 aacttcataa ccaggcattg cttcacgata agcttgcagg gcagaattat cattactgct
     6841 gcctacaata ggcacaaaaa cctttttatt caaaataagg gaattggtgt agggctcatt
     6901 ggaaggagta taaacccgat aaacacggta gggataaccc caggcacaat tatgcgtagc
     6961 aaaataattg gctgcatttt ctatggcatt atattgagaa tggcttgcag gaacgctacg
     7021 aatcattact ttatcgggtg ccaaaaactt tgcccaacag tcaatatgct caatataagt
     7081 gttattcggg tctgccatca ccaaataatt tgtaatacct aaatactgtt gcattttagt
     7141 gtttacattt gtctgattgt tgccattttc ggtataaacc agggttgttt gagcagcagt
     7201 attaattcca tcgctcatat aatttccccc tgtctgctgt aaattcattc cataataatt
     7261 caaagcaaaa tgattggcaa aaacctgggg aattaaattg tcattaggac ggggacgatt
     7321 ataaataaaa tccaccatac catattcacc attgccgtca aaaatgaacc agggtccgta
     7381 atcccttgtc cagtaagaat ccgtagctgc attgagaaaa ctaacccttt ccatatttac
     7441 tccagcattg gtaaaagtag aaatggcaga tgattgttgc gaactgctta cgatacaaat
     7501 tacatttgcc gtatttgcca attgcactac cagcgaaaca ggtatcccta aaggataacg
     7561 aattaaaaca tcagaggcgg gttcaaattc tgctatagga cgaaccggag ctacaggagg
     7621 attggtttcc gtaaaacgga tttccgcatt tgctttatct gcgggcaaat tatgatgcgc
     7681 aaaggcagta gaactcaaaa tacagagcac actgcacaat aataatatgc gtaaaatacg
     7741 ctgttttttc atcagcgttc actccttatt atattatacc aaattataaa taaaacactt
     7801 tttataccga aattatatat tttctctcct gcccttatcg cagaattaat tcacctttgt
     7861 tatgatgtaa gaattgcagg tgaactatag cataatccat tctttctatt tagcaaattc
     7921 ccttggcaac gaataattcc cagggttttt tcaagttata cgccataact tccctttttt
     7981 tgccacctaa attatcagga acgatttttt catacttgtt taataccaga taggtcttaa
     8041 aaggaagtcc tgataattct gccgcttttt ccactatttt gattcccttt agcacgattt
     8101 ccaccgttgt ttcatccatc aggttggtgt tacatataat ttctgtaatt tttaagcgtg
     8161 aagtggcttc caatgcctct ttcattgata ttatctcttc cggagttgaa gtaaaaggac
     8221 gcatagtatt tacaatatat aacatactat aaccccgtgt ttttatgggt tcttcaaaac
     8281 gccctaaagt tctgcaaccg gcagggtcac cacctacatc cagaataact gttttcgctt
     8341 tattttgaat agctccttta acacggggag aaatcatcgg caaatcgcta tgcttatatt
     8401 caccttccgg agcaataata tcaattcctt cctgtataaa atcatcctgc atttcccgtg
     8461 acctgaaata aggattgacc acatccaaat cagctaaaac aacatccaaa ccttgtgtct
     8521 taaaacgacg cgcaagatga atggaatatt cactttttcc gcttccgtaa gcaccgatga
     8581 tgatatataa gtgtgagggc ataattcaaa tccttacatt taattgaaac gggagattaa
     8641 ttcttgagaa aggtctaaat gcaaagacat caaagtttat ttccaggtgc ggaacaggtc
     8701 ttctgccagg gacttaaaat cccaataaaa gccatcaact atctctttta tcagggaatg
     8761 ctgatcagag ctatcttcat cataaatggc aaaaacggtc atcccggcgg attttcctgc
     8821 ttgcacaccc gttaatgtat cttcaattac caggcaattt tccggttttt cctgcaagcg
     8881 ttccgcagcc aaaagataaa tatccggata gggcttacct ttcaaattta tatctccggt
     8941 aacagcacac tgaaaatatt gccacacaga attccggata agtacttttt tagccagttc
     9001 aagagaatta cttgttccca gtcctatttt tatccctttt tcttgcagac aggaaagcag
     9061 ttctacaaca cctggtttca gcataatatc cgtttcataa tgcttgctga ccatttttgt
     9121 ccattcctgc ataatacttt caggactatc gggcaaacca aatctatcct taaagtactg
     9181 tgcggtttga atgaaactgt ttccctgagg aagctctgaa aaaagatcgg gaggaactat
     9241 aagaccgcgg gtatgtaaaa attccctgtc cacattacgc cagagctgca ttgaatcaat
     9301 taaagttcca tccatatcaa agattactgc ctggaaattg ttcatctaca gaaacatcct
     9361 ttattgtata acggaataat aaaactggag cgggaaacgg gatttgaacc cgcgacatca
     9421 accttggcaa ggttgcgctc tgccaactga gctattcccg cttggtacca gaggccggac
     9481 ttgaaccggc acgagctttg ggctcgaggg attttaagtc ccttgtgtct acctattcca
     9541 ccactccggc aaaataataa actggaggcg acacccggat ttgaaccggg gataaaggtt
     9601 ttgcagacct ctgccttacc acttggctat gtcgcctaaa aaattaactg gagcgggaaa
     9661 cggggtttga acccgcgaca tcaaccttgg caaggttgcg ctctgccaac tgagctattc
     9721 ccgcaaaata atcttttcag cagtcaccaa aaaataaatg accacttttc tgtcaatcaa
     9781 aatgtatctg cttcgctaaa gacatctgca gtaaaacgat atacaacgct ggcaggatca
     9841 agatatgcat tgggaggcaa tcccgcttta cgacagacct cacgcagaaa ggtctccggt
     9901 tgccaattgt attcaacagc cacctggggc aaaagtaaac cgctaccata aggatgctga
     9961 atgtaaagtc cgtctttgcc aattaccggt aactcgtttt tttgcatagg aataagttca
    10021 ctcaaaatag atatttcaat agttactttt tccaattcct tttctgttaa aggagggaaa
    10081 cgcgggtctt gaaaagcagc tgcttctgcc atttctttca cactttcagc aatggactta
    10141 taacccttta tgtaaccgat acagccacgt aaattatctc caatatgtaa acttacaaac
    10201 acaccgcgtt taggactgaa ggcaggatcc tcaggcaaag aaacggactg atgtttaaaa
    10261 cgagcactaa ttgcatttcg ggcaagggaa agtagttcct ttttctgttc caaagttaac
    10321 atttatcctc cttccagata ttaaatataa agtttagcag aaagataacc tactacctga
    10381 gaatcgtttc cggatatctt tcctgaatgg gtatattgaa ttactttcat tttggcatta
    10441 gtgtaatgct gtgcataata caacaggcag aggattcccc cgatgccgca tgcctcacaa
    10501 tcgctatgaa taatatcctg ccaaagatgt tccgggtcta aagcagtaac atcttttact
    10561 aaaataccat ccatttcttc agcagttcgg gcacgatggt aatgtgataa atccgaagaa
    10621 cagaggataa cgatacgctt tgtagaactg taaatcgttt cataaagtat atctgccagg
    10681 cgttttgcca ctgcaggaat ttgattacca aacataatag gcaagatttt tgccttgggg
    10741 aaaaagtaat ttaatatggg cagttgaatt tccatagaat gctcttcctg atgtaagatg
    10801 agggggatat tttgatctgc ttcaggagct attttgttgt aaagctccat atccaaatcc
    10861 aaagtgccta aaggatttac atattcctga tagggagaaa cgctgaaatc aaaaaagttt
    10921 gcttgatggc tgggatgaag aattatgaaa ctatcaatat tttcgtggga aatactatgt
    10981 aaacccaaag tagcacattc accggaatac atataaccag catgaggaag aataactcct
    11041 aaagctcttt ctgaagaaag aggagttaca gcattgctta tccaaccctc tatctgtcgc
    11101 ttgatctgtt gttcaaaccg gggatagaag gttccagcat gcataggatt gcgaatcatt
    11161 gttcacctcc ttgaagagga agatatatta tataagttcc tgattttttc tcttccgtag
    11221 ttaaatttgc tgtctgccaa ttttcgctat aggaagggct aataactgca atttttttat
    11281 taggtagaat tgactgaacc ctactgacag ttccggaaaa attacggcta tgaaaatcac
    11341 cgttaaaatg gagaacccgg gctttgggtt tcatttttag ggcattcaca atgctttctg
    11401 ccatagtttc atctttcaaa cattgagctt gataaagcca atccgggtta ttattcatca
    11461 tcggagaatg catatcttcc agagttgcca ggaatgcctt tttatatccg tcatcaggat
    11521 aatctatttt atcgggcaac cattttttat cttcttctgc caatgtttca gtaaagtcag
    11581 gtcctgttct tgccatttta ccagcgatag aacgaggaat attagcagct attacagtca
    11641 gttttttctg tttggcatat tcaatcaggg gacgatagtc atcctgataa ttactccagg
    11701 gacgactttt agccaaaaat tcatcctccg taagccaacc ttctatataa gcatccaaat
    11761 cactttggac atcgcgttca aacatttcca gagagagaat cagttctctt tttgtatcca
    11821 gcagaggaac aagttctctt tccagctgat ggagagtagc attatcgtga aattctccga
    11881 aaaatataat atcgtatttt tccaatcctt tagccatttg ttgcaatgta agagttttac
    11941 tattgcggga atccacaata cgatattcat ctgcatttaa tagagttaag ttcattatta
    12001 tgatcataat tactatgata tatttcattc tcacttcctt aatagttcta ctgataattt
    12061 aagcgaattt tgtgtttatt gccatcaata attttacgaa attccttttc ttgttcagct
    12121 ggaagattta gtataaagct aacttcttca gaccatatca cttgctttat ttctcctccg
    12181 attgccagaa aagaatgttt tagacattca aaagaagcat aatcacaaac aagaggaaaa
    12241 gaaattagca actttgcttc tattagatta cttttcagga ttgcttgttc aactgcttca
    12301 gaataagcag caatcagtcc ttttacacct aattttatac ctccaaaata acgggaaaca
    12361 acagccagaa tatttgtcag gtcatttcgc aaaagtgtat tcaatattgg ttttccagct
    12421 gttccagttg gttctccagc atctgaataa tagataattt ctctgtttaa accgtgaata
    12481 taggcataac aattatgagt ggcatcagaa aaaagccgat aatgctcatt caaaatatct
    12541 ttaacttctt ctacataggg aagaggatac aacactgcca caaatctgga gcgctttatt
    12601 tttatttcac tatcggtctt tttttcaatt gaatacagca ttttttaact cccgcacttt
    12661 gagctcctgc acttcagcac tttgtactcc cgcactttat ttcaccttcg gaaggtcatt
    12721 ccagtgggtt gtataatgag gtgaaagttt tgctcgtttc attttccagt ccttttttat
    12781 tccgctactg gcataaaaaa gagtgtcctg accataagtt ctatttagtt tatccattgc
    12841 ttccatcagt tttttacgtt tatcatccag ataatttgtg tccataaagc ttaagggaac
    12901 atctttttcg ggaataatat cagccaacat tactccggct tttttaaatt caaaacccgg
    12961 taaatataag tcatccagca ggttaagagc tgtttttatc agttcgggag tataagcagt
    13021 agggggaaag agagttgtag aaagagaatt gttgtattga ggtccttcct taaaacggtt
    13081 tgtagaaaga aacaccatca gcaatccagc cacacttttt tgtttccgca atttttctgc
    13141 agcccgagta acatattcag aaattgcctc ttgcaagtca ttaagagagg aaacctgttt
    13201 accaaaagag cgggaagtga caatactttt tttgtttgca ggagcttcat ccaaatcaat
    13261 gcaagcatag ccacgcagtt ccaaaacggt ttttaagccc atacttgtta aataatgctc
    13321 tataaattta tcctctgcat ccctcaattg gagcgctgtt tcaattttat gctgtcttaa
    13381 aaatttatca tattgccttc caattcccca aacttcagag acaggtgttt ttgccagtgc
    13441 tttggcaatg cgttcatcat ccaacaaaca aagcgaacct tgaaaagcag aatagcgttt
    13501 ggcaaaatga ttggcaactt tagccagcgt tttggtttgg gaaattccca ccgaaacagg
    13561 aattcccgtc cactgaaaaa ccgttctttt tatctttttg ccaaagttct ccaggtctct
    13621 atttttaaag ccgtttaacc ctaaaaatgc ctcatcaata ctgtatattt ctacttccgg
    13681 agaaaacaga gaaagaactt ccattactct tgagctcata tctgcataaa gagcataatt
    13741 ggaagaaagt aatgttccac cgtgtttttt aatcaatccc tcatatttaa atccgggtgc
    13801 tcccatagga atttttaggt ctttaatttc ttgtgaacgg gaaacgatgc aaccgtcatt
    13861 attagataaa acagctaccg gtttattatt caatttggga ttaaaaactc gttcacagga
    13921 tacataaaaa ttgttgcaat ccaccagagc aacaatgtta tcatttttta agccggtagt
    13981 attcattgtt ttcctttttc ttctgttctc tttttctgtg tgttacttta ttcactattg
    14041 cctattattg tcaagaaaag tcagtaaggc aagagggaga ttttgtttcc cgcagattac
    14101 gcaaattgca cgcagatttc accaataaaa atattgcaca cgaatttgac gaattttaga
    14161 ggattttaat ggataagggc gggagaatgg aaatgaagaa tataaaattc agaatgtaaa
    14221 atgaattatg ctcggtaaaa gggttaatat ctaaaaggtt gagattctta aaaagcccaa
    14281 aagagcgaaa ctggagggtt gatgtcccca tcaacttctt tttaaaaatg gctaaaatac
    14341 cctttgtcct tcgctttctg ccctctgcca aaagcacaaa agagcgaaac ttttatagcg
    14401 atggcggcgt aagcccctcg ggaaaatgaa agccccgact tctttttctt tctccctttc
    14461 ccactaaaaa aagccccaaa gggcgacaca atgattgaaa aattgcgata ttttcggtaa
    14521 cgaaatagag gttaaaagtt agttgaccag gaggtcaaac ttccttttta aaaatcatta
    14581 taacctataa atattaatct taaccttata aaacctaaat agataactga atattttgcc
    14641 catcctgaac tatatttatg taatagcttg ttatatagat gattaagtaa tgactccgta
    14701 acacttccgt aacacttccg ttgtctcgtt acggggttgt tacttggttg ttactttgtc
    14761 ataaaatatt caagcaggca agaatgctac ctatcctgat ttttcagaaa taggaattat
    14821 atcatatttt tatttataaa acatcaatgg ttgagaaaat cccggggaaa tagcaaaaca
    14881 aaaagctcgg attttaaccc tgagaatatt taaataataa agagaaaaaa tcccaagaat
    14941 agggcagatg aagaatatag agaaaggcaa tttatcccta aatggtaata attatcaggc
    15001 ataaaacgcg taattttatt agaggatata aattctattg ttctaatgtc gttcctgcta
    15061 aagccggaat ccagggaata ataaaaataa cttatgcttc cttttcgccg ggttcaaaac
    15121 ccgtcgttag tatctgtcgt tcctacggaa ctctttgtcg gaaaaagaga taatgactat
    15181 cctgctttct tttcgccggg ttgaaaaccc gtcgttagta tctgtcgttc ctacggaact
    15241 ctttctatta tttatgattt acaaatatgg ataaagtaat atttctcttt atctcttttt
    15301 ctctttgtaa aaatattctc tttatctctt tctctcttta taaaaatatt ctctttatct
    15361 ctttttctct ttgtaaaaat attctctttt tctctttccc tctttgtgaa agaaaaaaaa
    15421 ccgtcagctg ggggtgctga cggttagtga gctttggact tttgtttcac ataaacaagg
    15481 aggagtttat gatgaagcgt tttttaattt taatcaagtg ctttcaggaa tgaaggcaca
    15541 tctgttttca aaggaggaat gcgtgtaggt tcattcgcag ctggtttaac ttcctctttt
    15601 ggtgttgatt ggttaatact caaacgggca aagatatctt caatttcggc attagtatct
    15661 tcggattgag tgatagaagg gacagatttt tctccgaaat caggcaaacc ggggaagtca
    15721 atagtatgtt ctttatcttc tttttccagc ccggtggcaa taatggtaac ctgaattttt
    15781 cctgccatag catcatccag aataataccc ataatgatat tagcggcttt tccggtttca
    15841 cttacaataa cattggagac ctcatcaaat tcactcatca ggatatcata accggctgtg
    15901 atatttaaca gtaaggactg acatccctgt aagctgatat gcgataaaag aggattatca
    15961 attgcggctc tggctgcgtt tatagctctg ttttctcctt ctgcaattcc gctgccgata
    16021 agagcataac ccatattttg cataatagct ttcacatcgg caaaatctac attgataaga
    16081 cctgtaacat tgataatgtc gctaaccgct cgggctgctt cataaagaac aaattcggct
    16141 tttttaaatg cttcctttaa agtaaggttt gcatagattt cacagagttt ttcgttggga
    16201 ataatcagta aagtatccac gaattctcgt agatgtttaa tgccgtaaat tgcattttct
    16261 gctctttttt taccctcata ggggaaagga gaagttacaa ttccaaaagt taaaattccc
    16321 atttcacgag cgattttagc gataataggt gaggcaccgg ttcctgttcc acctcccatt
    16381 cctgcagcaa taaaaaccat atctgccccg tccagatggt ttttaatatc ctctttggat
    16441 tcttctgcac ttctggctcc aagttcagga ttggcaccgg ttcctaatcc gcgggtaagt
    16501 ttctttccca gttgcaattt catattggct ttactttttg taagatcgcg gatatcggta
    16561 ttagctgcga taaattccac accgaataaa ttattctcaa tcatagtatt gagtgcgttt
    16621 ccacctgctc cacctacacc gataatttta atgttggttc cgatttgggc gggtttttcg
    16681 tcaaattcaa tcattttatc ccccttttag gtaaagtcct ttaatatttt tttaacttta
    16741 tctacgattt tttttccttt tatgtttggc agagtaaagc ccttactaac gggttcgtta
    16801 tttttacctg ccgcatagta taaaattcct acaacggttg cataagcggg gtcttccaaa
    16861 cggctaatca ttccgtttaa acgggaaagg tctggtgtgg caatttttac atgaagattg
    16921 aatgcttcag ataaaacaac ctctatgttt ttcagtttgg ctgttccccc gcaaagaatg
    16981 atacccgaag ttaccagttc cggagtgtaa aattcctttg ctttattgta acaaagagaa
    17041 agcatttctt ctacccggtg ttgaataaca tggctaacta aaaatttggt tttgcggctg
    17101 gtagagcgtc cactaattcc ttccacatca atttcttctg tttggtcaac gctgctggca
    17161 agagcgtttc catattccac tttgatatat tctgcattgc ctaaagtggt cttaagtcca
    17221 attgccaggt cttctgtgat attttttcct gccatcggca aaacgaggat tttttccagg
    17281 gaaccgcgat tataaataga taggtcacaa gttccacctc caatatcaag aactaaagct
    17341 cccagccgtc gttcatcttc acttaaaacc gattcggaaa ttgcaatatg atttaaaata
    17401 aaattttcag gattgatttc ataacctgcc agctgaatgc acttacttaa attgcgcaaa
    17461 ggggtaagtt ccgctaaaat aatatagacc ttggcaatta aatgaaatcc attcatattc
    17521 actggattgt gaatatcatc ctgattatca attacaaagt tatgcggaat tccgtgcaaa
    17581 atgcgaaaac gctccagacc tttctgaatc ttaacgctgt ttttggcatc attgataacc
    17641 tgttcaatat gttcttgagt aatttctcct ggttcattcg gtgtttcgga aggaatggaa
    17701 atacgtccat ctccaaattg agtccgaata tgttctccgg tgatattgca atagatatta
    17761 ttggcagagg tttttccttc tttttccgct gtagaaattg cttttgctac acaaccggaa
    17821 agtgcctgaa tatctttaac aattccacct tcaatgcctt cggaaggata ttcgcctaaa
    17881 cctaatattt ccagacgtcc atctccaact tctttggcaa tgatacaacg aacattggaa
    17941 gagccaatat ctaaagcagt tatgattgat tgtttcattt attacctgcc ttaactacaa
    18001 cctgattttt atagcgcaaa tcaactacgc tatttttgtt tatattgcca ttttcctgaa
    18061 caaactgata acgtgcaagc tgtttagcta agtcctcctg agcaggaata atgcgtgtac
    18121 ctgttttcgc atcaataata ttaaccgtct tatctattaa ataatattcc gaaatctggg
    18181 gaagaaaatc cggagcttct ttggtaatgc gtttatgcaa ttgtaatacc tgttgcaatc
    18241 ctgcattttt cagtttatgt ccaggtttta tttgatggtt acttaaatag gtgctgtaaa
    18301 ttggcatatc ttctttataa ataggggaat acttcatcaa aacaacagcc tcggaatcaa
    18361 ttggatgcaa atcaccttca attgttttca gatatattgc cgctttgcgt tcattgattt
    18421 ccagttttat ggttgaaggt aaacgtttat gtaatttaac atcctttacc cgggaaagat
    18481 tcattacctt atttttaata tcctctgcag gaattgccag taaattcatt ccaatatagg
    18541 gttgggtaat tttgtaaatt aaagtatcgg gaattgcctc gtttccaata acagatactt
    18601 tttgtaaagt gaaaaggtct atatgcgtaa gaccaaacca gatacccgtt cccaaaagcg
    18661 ctacaccaat taaggcaaaa aagaagaaca ggtaataacg actattaccg cgacgcttac
    18721 gagtaccttt gtttagttcc ataagtttat gtatcaaagt cctttagctt actaacggta
    18781 aaatttgaaa aactgatttc agctttacaa gttactaata ttttattgct tatgatttgt
    18841 gtcaacataa ttcttcaatt gccttaaaat ttcttctcca tactgccaaa tattgcctgc
    18901 tcccatagtg attaaaatat ctccttcttt aagaagagca agggttttgc tcactatttc
    18961 ggaattggag ttaattaaat ggacattatg atgtccactt tgaattgcag catcagcaat
    19021 cagtttggaa ctaattcccg gaataggttg ttcccgtgca ggataaattg gagctaaaat
    19081 gaggcagtca caagaaaaga aggactttcc gaattcctca taaaaatcac gggtcctaga
    19141 gtataaatgg ggttgaaata gagctacaat tctccttttg gcgctatctt taaagccctc
    19201 caaagttgct ataatttctg taggatgatg cgcataatca tcataaatgg taattccacc
    19261 tgcttcaccc ttcaaatcaa atcttctgta aacaccgctg aatttaccta agccatcttg
    19321 gatagcggga aaagggatat ccaattccag accgatggat gcagccagta atgaattttg
    19381 aatattatgt ttaccggtaa ctttcaaaga aatgcgtccc agcttataat ccttaaacaa
    19441 aaggtcgtag gaagaattga aaccctgcat atcaatatct cttgcttgca catctgcctg
    19501 acgagagaaa ccgtaggtaa ctattttttt atgaataaaa ggtaagattg cctgcacacc
    19561 aggatcatcc aaacaggcaa taacactgcc aaagaaagga actttattgg catattctat
    19621 aaaagagctc ttaatttcat caagattacg gtaacaatcc agatggtctg catctacatt
    19681 agtaatacct gcaatacaag gggttagggt taaaaaagaa tggtcatatt catccgcttc
    19741 tactacgata tattttccac tgcccatcac attgttactg ccaaaatttt tcacttttcc
    19801 cccaacgatg atagttggtt ccaaacctgc actttccaaa acagaaccag ccatagaagt
    19861 ggaagttgtt ttgccatgag ttccggcaat tccgatagaa aaactcattc gggtaatttc
    19921 agccagcaat tctgctctgc ggataacagg tattttcatt tcgttagcag atataatttc
    19981 cggattgtca tcatttatag cgctggattt tactactaca tctgcatcgc cgatgttttc
    20041 ggggttatga ccttcaaaaa tctttatgcc gagggattcc aaatgagttg taatatccga
    20101 ttttttaaga tcggagccgg taatttctaa accctgattg tgcagaaatt cagcaattcc
    20161 gctcattccg atacctccaa ttccgacaaa gtgtattttt ctggttcttc ctaacattct
    20221 tcctcctctt tactattaag atagtttaga atgtactcaa ttattttttg ggctgcctga
    20281 ttgggtggaa gcgaagctaa cttattccgg taatattcca gattttcagt tatttttttg
    20341 atgttttcta caagggaaaa gggagttaat ttactttgtt ctaaaagcac tgcaactcct
    20401 ttttcctgtt gagatagagc attataatac tgatgatttt cagcagctgt aggcagagga
    20461 ataagaatag ccggaagacg attttcttcc agttccgcaa tagtcatagc tcctgctcgc
    20521 gtaatagcta aactgcattg ggaaagcatt ttgggcaggt caggtgaaaa agcaaaaata
    20581 taaacacctt cccttccctc atgtttggca gaaaaacgct cgtaacttat ttttcccgtt
    20641 tgccaaataa tttgatagcc caaagataat atatccgaaa ttgcctgatt gacaactgtg
    20701 tttatagcaa cagaaccctg acttcctccg ctaactataa gcgttggttt atcgtttttc
    20761 aagcccaaag agctaagttc aaaaggtttc tgatccttgt ttgttatctg tctaagcggt
    20821 attccatagt ttaccaattt tgcacggctt aaatatttcc tgctgttttc aaaggaaatg
    20881 aaaatagtgt taatccattt tgccatttta cgggttacca agccggggta actattactt
    20941 tcatggaaga acaaagggat ttttaataag gcagcagata tggctacagg accagctaca
    21001 aaaccaccgg tgcagataac tgcctgcggt ttttcttttt tcataatgtt tacagcagta
    21061 ataacactgc ttaacaaata aaagggaacc agcatattct ccggttttaa ggaacgatgt
    21121 aatttctgca ctttaatctg ccgaaaggga taacctgccg aagcacaaag cgtttcttca
    21181 atgctttggc gattacctaa aaaaaggaca ttatgcttat accttaccaa ttcatcagcc
    21241 agtgccaatg caggagtaat gtgacctcct gttccgcctg caccaaaaat gaatttcata
    21301 catatctccg ctgagcactg atatttagaa gcattcctaa agcgatagaa tcaataagta
    21361 aagccgatcc accataactt ataaaaggta aagtatttcc tgttggcggt aaaatggaca
    21421 ttgctactcc ggtgttgact aacacattgc aaaagatatt cattgccaag cccacaccta
    21481 aaaaccttaa aaatcggttt tcttgtgaat ttgccatccg gaaacaacgg aaaaagagaa
    21541 aacaatgtaa accaaaaaca attagggctc caataaaacc ccattcttct ccaattacag
    21601 tataaacata atctgttctg gcttcgggaa gataataatg ttttgccctt ccttttgcca
    21661 taccggttcc aataagacct ccactggaaa gagcggttaa actttctttc acttgataat
    21721 cactatcctc aattttgatt tcaccttcgg gtcttaagaa taaactaaat ttcttgtaag
    21781 ttgttaaacg tccctttcta taatctgctc cattggctaa aatcaatacg ccagctatga
    21841 tgcctataag agctaaagaa ataagcacgc gttttttagt gccagcataa ataagcatag
    21901 catatagagt taacccacct ataattagag tacttaaatg gcgttccata ataatcaaca
    21961 ggaaggttat gcccgttatt actattaaag caaaaaaatt agtaaaaaag tgaagtggat
    22021 tggaagcaac aagttcatca tactttttat ccaatatgtg agcaaaataa aagaccaatg
    22081 ctaaccgagc caatacactg ggctgaaaat taataaagcc caaacttagt tgacgcgtag
    22141 cacctttaac tgtgcttcct tttagcagaa caataattaa caggatgata gttagataaa
    22201 caaaataagg agaaagaact cttaattttt ccagattaaa agtataaaga attacaatca
    22261 ctgtgatcat tgatataatc aaaaatacga gttgcctgta aaaatacttc atagaactct
    22321 ggacagaaga aatatccagc aaggttatta aacctaccag acagagcaga caataggtga
    22381 ttaaaattac cttatcaaaa ctgacgatat aacttggtgt cttatttctt ctcattttct
    22441 ttgcctattc gggctacaat ttccttgaag aaatttcccc gctcctcata attattaaag
    22501 cgatcaaaac tggcacaagc aggtgaaaga acaattatat ctccttcacc agaatcagaa
    22561 aatgccttgc gcacagtcaa ctcaaaatca gcaatgcatt ccaaagggat tttaccggag
    22621 aaggttttac gcattttttc ctgggtatca cctgtcagat atgctttttt cacttttttt
    22681 tgcaaatcag cagcaagcac actataatcc tctcctttat cagaaccgcc cataataata
    22741 cgaatcggtt gctcaaaact gtttaatgca ctgcgcacag aatcggtatt ggtagccttg
    22801 gaatcgttat aaaaagaaac accttttatg gtatctacat attccagacg gtgcggaagc
    22861 gatttaaaac cctttaccgc ttcggcaatt tttgtcggtt caggaattaa agcataaact
    22921 gccagcattg cagacatcgc attggcataa ttatgcggtc ccttaatggc aaaataagaa
    22981 agcggaactt tggtttcgct gccaaactga atatagttat ttgccagata agcaaatgcg
    23041 ttatcgtttg gggacaaaga gaaatatagt ttcctggatt ttatatcctt agtgaaatgt
    23101 ttaacttcag gggaatccca aaacaggact gcagtatctg tcatttcttg atttttgaaa
    23161 atgtttacct tacttaaagc atagtcctca aaggaattat agcggtctat atgatccggt
    23221 gtaatattca aaagcacggc tacatcgggt tggaaactat taatcaggtc taactggaag
    23281 ctgctgattt ccaaaacgat atagttccac ccgggttttt ctatatcata actgcaaaag
    23341 gcgtcgccga tatttcctgc taaaagggat tttttgccta atcctgtaag aatatgatga
    23401 attaagctaa cggttgtgct ttttccgtta gaaccagtaa cagcaataat tttagagtcc
    23461 tttgccttta ttctgtaacc gagctcaatt tcactaatga gttctatatt tttttctttt
    23521 gccttttgca gaataggtgt atttaaagga ataccggggc taactatcag acaatcacat
    23581 tccagcagtt tattggtatg acccccaaat tcacagggaa aatccttttc cagtttttct
    23641 gctgtaggaa tttcttttgc cgggcgcaat tcacttaaga agggagtaac gccaagttct
    23701 ttcagcttat aggcagaagc gaatccgctg cgagccaaac ctataattcc gtatttttta
    23761 tctttatcta tcattcta
//
LOCUS       DAOPFL010000019        23524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2245_length_23524_cov_97.733393, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000019 DAOPFL010000000
VERSION     DAOPFL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 23524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23524
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_23524_cov_97.733393"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1443
                     /locus_tag="PLG69_02665"
     CDS             178..1443
                     /locus_tag="PLG69_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42441.1"
                     /translation="MRNGIKIIIVCLFCTLTWSMQGETISPFSLKDKTFEARADFGIE
                     TLKNRNFSFWEGNSSFHYPPNIQAEMDYDSYSSFPEWSKNVTFHSAQEFLYLDVPKSG
                     GNIQEFNSFNTIGVKSRLAGVEVFYNLINNRNDTDEKVTSYHFNKYGIGAGFPNSESL
                     RLFGWLMHTDLSDIYPEVDFSSYKAQMAKRYRSKSQIAELGMEYEYNEIPDKVSEEEL
                     IKVSEYFPDDYLDWTPKKRFSAYVYASSIRNFYLEPFFLNGHAPDCSPLLLADKSASS
                     VLCRFTTEDRKNDYVDNSQIVLDGILALNFGKYFGLDFRYDSEKKEDYKNDIKENTFN
                     ITGNMRLNFIGKESYRLIGILEYLYNKTKGENASENLNFGLYGILRLKNHLNLIAYLK
                     LDSEWQRKDDFLEFDHRDNKLGTILSVRL"
     gene            1504..1887
                     /locus_tag="PLG69_02670"
     CDS             1504..1887
                     /locus_tag="PLG69_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPI42442.1"
                     /translation="MKVLKTFPDKCITCHNCESACSKLYFKEDNAEKSCIVINEEVYP
                     PEMIVCNQCGNCIDICPTLALTINTQGVVMLNKSLCIGCLMCVAACPNNAMRFAKGVY
                     NPFKCIACGSCPKTCPAEAIELVTA"
     gene            1898..4030
                     /locus_tag="PLG69_02675"
     CDS             1898..4030
                     /locus_tag="PLG69_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPI42443.1"
                     /translation="MNRKLISEFKYDLAPVIRGYNKRSLYINLSTNEIKQKPVTELMI
                     DKFVGGKGFDLYLMWHSVQDDTKWDSPENEICISFGPLCGNTSYPGSGKSIVTTISPL
                     TGIPVDCNVGGHFGPYAKFSGWDAIELQGIANEEVIIYIDGDKGIVQILSAPEEEINS
                     HILAEELIKEFADGPDKYQFVSVVSSGKAAENVLITCLNFSFWDKRRKVARLKQAGRG
                     GTGSVFRHKKIKALVVKYSGLKVDSNNPEDAETLRMTGLKLHKEIEAGDATQNRMRQV
                     GTAHLMEIMNDYDLLPIRNFKYGQSPEAEGLHSREFKNLFTQGMPDGCWYGCSLSCCK
                     GVDNFEPRTGPYKGQKVCVDGPEYETAAGCGSNIGVFNARDVVEINFYCDTYGVDTIS
                     FGTSTAFAMECYENGILNKERTGGLELTWGNADAALELLHQMAKGEGFGVIVGQGVRR
                     MKQIFAEKYGADPNFLQDIGMEAKGLEYSQYMSKESLAQQGGYTLALKGPQHDEAWLI
                     FMDMVNNQIPTFKDKAEALHYFPMFRTWFGLQGLCKLPWNDIEPANNAETDEPAKVPE
                     HVQNYVDIYKAITGKPLDKVSLIEQSERVYNFQKVFCLRMGKGRRIDDVPPYRAVGPV
                     TEEEYLSRQERYDQQLKEKIGVDPEGKSTAEKMDILRKYREDQYQQLIDAVYERKGWT
                     KEGVPKIEHLQKIGMDLPEVIEVVKRFL"
     gene            complement(4224..5558)
                     /gene="radA"
                     /locus_tag="PLG69_02680"
     CDS             complement(4224..5558)
                     /gene="radA"
                     /locus_tag="PLG69_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPI42444.1"
                     /translation="MATIFFCTDCGFETGKWSGKCPACGAWGTLKESTRVTGKNNKPV
                     ETLPLSKPERIIDLEYDEFSRLLTGIKEFDLVLGGGIVKGMLILIGGEPGIGKSTLML
                     QLSEWMGKQNKKTLYCSGEESTEQIRLRSKRLKVTSENIYLLCTNNAEHIIEAIEENK
                     PDLVIVDSIQSVSIPSLDALPGTVTQLRETTSRLLRTVKNLGIPLFLVGHVTKEGLVA
                     GPKIIEHMVDTVLYFEGEQRSQYKILRAVKNRFGSTNEIGLFEMTHLGLIEVSNPNNI
                     FLSHEQNDIGTAISCIMEGSRSFIVEVQTLVNASNYGTPQRVAVGLDQKKLAMLLAIL
                     EKNLSLYLRNCDVFVNLTGGIRSTDPSLDLAILAALISSMKDQPLIEKAVFIGEVGLN
                     GEVRPVSQLDKHISQTLKLGYEKIFISSYAKVKANPKVVKVKDIKALYGALR"
     gene            5760..6026
                     /locus_tag="PLG69_02685"
     CDS             5760..6026
                     /locus_tag="PLG69_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPI42445.1"
                     /translation="MSAKVIVFSTPTCIWCKKAKEYLKSINQNFTDIDVSKDIAARND
                     MIRKSGQEAVPQIWINNIPVVGFDLEKINRLLNLNNTKKGESNG"
     gene            6019..6474
                     /locus_tag="PLG69_02690"
     CDS             6019..6474
                     /locus_tag="PLG69_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPI42446.1"
                     /translation="MADYAKKFHELITRLQMVLSEIDYAQKACLQAGRMECMLLNYLY
                     KVNGPANMNELAKELNVSHSRITRIMDNLVAKQLVIREPSEEDRRCWFARITEKGKKL
                     AANSLQTVLDQQAKILKKIPEKDIEAIYKALKLYVDKYEEVLKETIAGL"
     gene            6474..6905
                     /locus_tag="PLG69_02695"
     CDS             6474..6905
                     /locus_tag="PLG69_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPI42447.1"
                     /translation="MSTYVKTVWSGGMSFTAEVNGHKLIMDADPYFGGTDQGPRPKPL
                     LLAALSGCSGMDIVSILEKMGIKDYNLEMDARGESATEHPIIYHTITLTYNFQGNNLP
                     AEKIVKAVKLSLEKYCAVNAMLKKAAKIIPKIYINNLEVPL"
     gene            6902..7426
                     /locus_tag="PLG69_02700"
     CDS             6902..7426
                     /locus_tag="PLG69_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPI42448.1"
                     /translation="MKWWLLIILSLSLAFLGCEKTDQPTINEDGTKDNGKLESGGKNP
                     ESKTGVWLTDWNDAMKTADVLNRPVLVDFTGSDWCIWCKKLEEEVFGKGEFLEYAKKN
                     LVLLKVDFPRDIEQSEELQKFNEQKMKKYGIQGFPTIVLVNSKGEEIARTGYRPGGAK
                     EYVKHLQELLQPKE"
     gene            8054..9382
                     /gene="ssnA"
                     /locus_tag="PLG69_02705"
     CDS             8054..9382
                     /gene="ssnA"
                     /locus_tag="PLG69_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HPI42449.1"
                     /translation="MNRYILKGGTILTFDSSHPVLEQAEILIDDGYILQIGSEKSFPD
                     EVCERIDVEGKIIMPGLINAHHHFYSTFVTGLGKAAPAKNFNAVLENLWWRLDKKLLS
                     EDNYYSALISILTSIRKGTTTIIDHHASPFAVKGSLSQIANAVQETGIKASLCYEVSD
                     RDGDKIAQEGIEENYNWIKNCQNNPNPYLKGLFGLHSAFTLSDKTLSIVADKVRELNC
                     GIHIHTAEAEADEKYNLEHYGKRVVERLSDFNLLNNKSILAHCVHLNPKELILVAEKG
                     AAIVTNPQSNLNNAVGIADVCKMTELGVTVGIGTDAMTNNMLEEVRVGIWAQHLKQND
                     PSRGFVELASALVFNNPLIAQKYWGAKFGTITAGSPADIIVVDYIPHTPFNKETWLGH
                     LIYGISQANVEATICQGKILMWNGELLLDIDEQEVKAKARELAEKLWDRF"
     gene            9367..9531
                     /locus_tag="PLG69_02710"
     CDS             9367..9531
                     /locus_tag="PLG69_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42450.1"
                     /translation="MGQILKSEINFFTMSKREEIRMKNVQKGKITLLELEITLGIKNK
                     FAESALNFLP"
     gene            9551..10750
                     /locus_tag="PLG69_02715"
     CDS             9551..10750
                     /locus_tag="PLG69_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPI42451.1"
                     /translation="MYREIFARAKHYEDTSTRCLMDMVKIPSFSTKEKEVAECILKEM
                     QEIGMDEAYIDPLGNVIGRLGFGKKVIAFDAHIDTVYPGDLSLWDFDPFAAHIKDGKI
                     WGRGTVDQKSGMASMLTSARIMKDLDLGKEFSIYFTGTVMEEDCDGLCWQYILQEEEI
                     KPELVVITEPTNLNIYRGHRGRMEMEVSIKGLSCHGSAPERGDNAIYKISRIALEIEK
                     LHQNLRYDDFLGKGSICVTQVFFTGPSQCAVPDSARIHLDRRLTFGENKESAVAEVQD
                     ACKKAGYPDATIEILTYQEAAYTGFVYPTEKYYPTWVTPLDSVYVQNAADAYTKTLGT
                     KPLIDKWTFSTNGVSIAGMKGIPCIGLGPGNEIYAHSPNEACPIEHLTKAAAFYAALV
                     YRFSQEK"
     gene            10893..12074
                     /locus_tag="PLG69_02720"
     CDS             10893..12074
                     /locus_tag="PLG69_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPI42452.1"
                     /translation="MKSYLTKYRCIECGKEFDPNELHYLCDECGRDYLPGMPLKGVLE
                     AVFNYEEIGRKWKQNPDPLLFSAVNPKFYPPLPVGNTPFFRVNIPGLKSGGTASSSYT
                     KWWIKFDGLNPSGSYKDRASQLVVADALQKGIKEIVTASTGNAACSLACLGASAGLKV
                     VIFAPQNAPPAKLIQIQVHNAELHKIDGSYDDAFKAALEYTKTHNCMCRNTGYHPLTI
                     DGKKSAGIEIYVQNGYKVPDWIVIPVGDGVILTGIYKAFLDLKKARITEKMPHLLCVQ
                     AESSDAITSYWETGIYHDAENPITIADSIKVKTPALAHWSVKALKETDGKCIRVSDKE
                     IQEAQLELAKETGVFGEPSSSATLAGLKKVVNHSWLKDAKDIVLLITGHGLKDPGAVK
                     L"
     gene            12071..13168
                     /locus_tag="PLG69_02725"
     CDS             12071..13168
                     /locus_tag="PLG69_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HPI42453.1"
                     /translation="MKASGIYDFHVHIGETIGGHLLADSWKSFNLLYEHNGLEGIGVF
                     VTESQSEPLSSKLARMRKASRSFLGKVFWHLTPRQLDVRRLENILKEDTDLKLYTTYR
                     EAGLYQSYESIEHLMEEMPSPKKRLLVHCEDDEIISEYSERYPFHNPSDHCLRRPEKA
                     EISAVEKLLDLSIKHHYPLHIVHISSPQSALLVQQAKKEADYITCETAPHYLLLNEEV
                     LNEPEGHRWLCSPPLRSESSRGLLLELLQDGVFDIIASDHCAFSSETKDVGILYPAKT
                     PMGIAGSGVLFTLLAEHLVEKGKLSMEQLFNFISFNPAKLMGFSVQNDKLSYERLGAP
                     IPVIPSWANTPNPWIDFWSYYELRRHHNDVY"
     gene            13155..15422
                     /locus_tag="PLG69_02730"
     CDS             13155..15422
                     /locus_tag="PLG69_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPI42454.1"
                     /translation="MMFTDAFRNDAAAKVTGRAKYTDDYKLPDMLYAVPLHSQVASAI
                     IKSIDYSEALKQKGVVAVYTAKDIPGKIKFGQIIKDCHTIINRRIRSAGDVIALVVAE
                     TREIALSALPLIKVELEEIPAVYNPEEAMQEKSPLVNPDFGSNICNYHKVRTGDIEQG
                     EKEADIIMEQEFNTSRVEHTYLEPETALCYLRPDGVMEVLGSMQHPFSTRRFVASTLG
                     CKLSEVEVKNIPMGGGFGGKDDTAALVCARAALCAYLTKRPVKLTYSREMSMRESYKR
                     HPYKLQYRMGLKKNGKIVFIKARIIADGGAYCSVTPWVTWRSTVQSCGCYEVPNVQSD
                     VYGVYTNNIFSGAFRGFGSPQVNFAIEQLIEMAAQKLNMDETEFRKINMVHQGSKTIT
                     DQKLDNHTVSLQEVMDSVLKEIDYENKVKKCSFGNPKIDEWYGIGYAISYRGVSLGAE
                     GTDMNSAIIYIQPDGSVLLETGVHENGQGAQSAMILIASEELGIPKELIRYRLPSTSN
                     IPDGGSTVASRGTIMGGGAVVNAAKIIKQRLCEGVEKALGIKDCSYKDGNVYFVQGEK
                     VVDYFEAVRLCYDHQIYPYAFGVFQAPPISWDEETGHGNAYFSWVYSCQAAELKVNSK
                     TGKITLLNLVAAHDIGRAINPALLAGQIYGGMAMGAGYALYENFPLVNGEPKASNFNN
                     YRVMRSTDLPEMTAIFVENPDPNSPSKAKGIGEPALEITAPAIANALYRATGKRFTDL
                     PMAPQVWEYVKGKQK"
     gene            15419..15934
                     /locus_tag="PLG69_02735"
     CDS             15419..15934
                     /locus_tag="PLG69_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPI42455.1"
                     /translation="MKITVNNIEYTVSENLAELKLSAYLRENLDLTGTKIGCDIGVCG
                     SCTILVNNKPMRSCLLKLKDVADKQVLTIEGLASSDGTLHPIQQAFIDAGAIQCGFCN
                     PGMVLTAYAFLLENKQPTREEAREAIKGNICRCTGYQQIIDAILLAAHRMQKRLDTEN
                     TEIFDNLTRIL"
     gene            16033..17196
                     /gene="ygeW"
                     /locus_tag="PLG69_02740"
     CDS             16033..17196
                     /gene="ygeW"
                     /locus_tag="PLG69_02740"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HPI42456.1"
                     /translation="MEKINTLINELETLKPDLFGKDFLLTWDNSLDSLKAVMLVAEIL
                     QNLHWQKKPWRIFDYGLAISIFRDNSTRTRFSFASAVNGLGLALAELDEVKSQIAHGE
                     TVRETANMISFLTEVIGIRDDMYPGEGHTYMLEVANAISEGYQQGILAQRPAVINLQC
                     DLDHPTQVLSDLLKLKDYFGGWENLKGKKIAMSWAYSPSYGKPLSVPQGIINLMTRFG
                     MNVVLAHPEGYDLLPETIQSAFSFAQESGGSFRQTTLMEEAFQDADIVYPKSWAPMSV
                     MQQRTKLLKAGDKQGLKELELHCLAQNEKYIDWECDDEMMKLTKNGNALYEHCLPADI
                     SDVSCENGEVAKSVFEKYRLHTYQEAGYKPFVIAAMIFMSKVKEPAAKLRSFV"
     gene            17444..18397
                     /locus_tag="PLG69_02745"
     CDS             17444..18397
                     /locus_tag="PLG69_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPI42457.1"
                     /translation="MKNILVLGAAGQIGSELVPYLRKLYGSNAVVAAYRSTPLTMEIQ
                     EGGPCENLDAMEADKMFEVVKKYSIDTIINLVAVLSAKGEANPLNAWNINMGSLINSL
                     EIMREVKGAVFTPSSIGAFGPSTPLDNTPQDTIMRPTTIYGISKVAAELWSDYYFLKY
                     GVDTRGVRYPGIISNVTLPGGGTTDYAVEIYYEAIKNKHYTCYLKPGTYLDMMYMPDA
                     MRACVELMEADPKKLKHRNCFNVTAMSIEPEMIAKEIRKHIPEFTIDYQIDPVRQSIA
                     ETWPNSMDQTAAMEEWDWKPEYDLPAMTLDMLKVLSQKLKK"
     gene            18394..19395
                     /locus_tag="PLG69_02750"
     CDS             18394..19395
                     /locus_tag="PLG69_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPI42458.1"
                     /translation="MIKIGVVGVGHLGQHHAQKFQNIDGAELVGIYDINEQRAKEIAK
                     KLNTRSFNSYEELLTACDAIDISATTTAHYELAKTALLAGKHIFVEKPITSELAQAND
                     LLKLASERNLIIQAGHIERFNPVILKIENEINNPLFIESQRISTFQPRGTDVPVVLDL
                     MIHDIDLILSFVHSPVTDIKASGVGIFTSTIDIANARIEFANGAVANVTSSRVSTKQE
                     RKLRFFQNDAYIRMDFLAKNVRVIKKSSDINSLLPQILMGAPNINPEQLLDIKSYDAD
                     DINKDALTLELESFIDCLKNNKKPVVDGEAGTRALEIATKIINQIQTQNTRINLLHR"
     gene            19402..20697
                     /gene="asnS"
                     /locus_tag="PLG69_02755"
     CDS             19402..20697
                     /gene="asnS"
                     /locus_tag="PLG69_02755"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPI42459.1"
                     /translation="MQDVFVKDIEKYLGQEVRLKGWVRNIRHSGKLLFIILRDGTGEI
                     QTVAFQPDLGEEAFETAKHLTLESSVIICGIPKLHPKQEGVYELQVTQVQPLQIADEY
                     PIGKKEHGPDFLLSNRHLWIRSQKQWALLRIRHTVYYAICNYLNEHNFIRFDSPILTP
                     NACEGTTTLFELDYFDEGKAYLSQSGQLYLETGIMSFGRVYDFGPVFRAERSKTRKHL
                     TEFWMMDAEAAFVEHDENMQIQEDLIRYVIQTVLSECALELQILERNTEALKAADAPF
                     KRMTHYEAVELLRQKGSDITHDKDLGAQDEVLLTEDSEVPVFVERWPKEIKAFYMKRD
                     PLNPNLVLGDDLIAPEGFGELIGGSQREDDYELLLSRMQAENMPIDDYQWFLDLRKYG
                     SVPHSGFGVGLERLVAWLSGTPHIRETIPFPRMIYRIYP"
     gene            20790..22004
                     /locus_tag="PLG69_02760"
     CDS             20790..22004
                     /locus_tag="PLG69_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPI42460.1"
                     /translation="MITNYFSETTKTMKSSLIRELVATTKNVPDLISFAGGFPSPATF
                     PTDILSKLFQEVVFQQGRDVLQYGNSEGDDQLKEEILKWENYPNLNRNNLIITVGATN
                     AIYYMSRTLLNVGDVVLCEAPSFLGSIVAFEAVGAEVHCVSMDNEGIDLNRLAEKVKR
                     LRDADKKIKFLYTIPDFQNPTGITMSYKRRYELLKYCEAEDILIMEDNPYSRLNFSEE
                     ELPTLLRIAQTELNNPYLVTEIVSFSKILGPGMRVAYAIGAPELINKMVSWQQKVTIS
                     PDCTTERVVAKFLAEGYMEPHIKNLCKFYKPYLDKMLACLSAEMPVYVQWTKPKGGIF
                     IWLWLPEDINADTLFYKAKDHLVTFIPGSKFYPEGQERFNCMRLNYTYSTMEQIETGI
                     KRLGELIKSIYV"
     gene            22065..22508
                     /locus_tag="PLG69_02765"
     CDS             22065..22508
                     /locus_tag="PLG69_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42461.1"
                     /translation="MRKVLLITMLLVMLLPLIAQSDIPHPNFSPYTNNGSSFLGLNKL
                     SMSHSMGFQAGTSSTGEGYYLSLYTNHLKYKFNPKLEMNLDLNFVNYGGFDTSSKFEL
                     DNANYSRVIPEFSLRYTPRDNIQIQLQMISGSILNPYKNNYLQNW"
     gene            22552..23280
                     /locus_tag="PLG69_02770"
     CDS             22552..23280
                     /locus_tag="PLG69_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPI42462.1"
                     /translation="MGCALGSFFNVLIYRIPKKQSIIFPGSHCENCKRPIPFYQNIPI
                     ISYILLKGKCSKCGAKIHWHHIVVEIITPLLFLVLFFRYGLHDFRFYKYILLFSFLIP
                     IFFIDALHQIIPNVLSIPLLITGIIFVFIPGNDVGIINGLVTGAVVFCLLLFLAWAYQ
                     KIRGTEGLGGGDIWLFTALATYFGLTGIPYIFLLSALMGIIYFLIFIRDKSQPFAFGT
                     FIALAAVFWSVIGEEIVFQLINFI"
BASE COUNT         7441 a         4001 c         4950 g         7132 t
ORIGIN      
        1 ttcgtttttc tctcacagat ttcacggata acacagatat atattagaaa ggatttataa
       61 ttttccattc tcaatttttc attaaagcaa tcaccatttc tctatctttc cctcaaaaaa
      121 ccattgacag caacagccag ccctaaaaat aagcattatc ttaaagagga gtaataaatg
      181 cgaaacggaa taaaaattat aatagtgtgt ttgttttgca ctttaacttg gtcaatgcag
      241 ggggaaacaa tttctccttt ttccttaaag gataagacat ttgaagcgag agctgatttt
      301 ggaattgaaa ccttgaagaa caggaatttt tctttttggg aaggcaattc ttcctttcat
      361 tatcccccaa atattcaagc ggaaatggat tatgatagtt attccagttt tcccgaatgg
      421 agtaaaaatg tcacttttca ttccgctcag gaattcttgt atcttgatgt gccaaagagt
      481 gggggaaata ttcaagagtt taattcattt aatactattg gtgtaaaatc cagattagca
      541 ggagtagagg tcttctataa cttgataaat aaccgtaatg atacagatga aaaggtaaca
      601 agttatcatt ttaataagta tggaataggt gccggttttc ctaattcgga atcattacga
      661 ctttttggct ggttaatgca taccgattta agcgatattt atcccgaagt tgatttttcg
      721 tcctataaag ctcaaatggc taaacgctat agaagtaaaa gtcaaatagc agaactggga
      781 atggaatatg aatataatga gataccggat aaagtatcag aggaagagct aattaaagta
      841 tctgaatatt ttccggatga ctatctggat tggactccta aaaaacggtt tagcgcttat
      901 gtttacgctt ccagtatacg gaatttttat ctggaacctt ttttcctaaa tgggcatgct
      961 cctgactgca gtcccttatt attagccgat aaatctgcca gtagtgtgtt atgcagattt
     1021 accacagaag acagaaaaaa tgattatgtg gataactcac agattgtttt agacggcatt
     1081 cttgccctta atttcgggaa atatttcggg cttgattttc gctatgattc cgaaaagaaa
     1141 gaggattaca aaaacgatat aaaggaaaat actttcaata ttacaggaaa tatgcggtta
     1201 aattttatcg gaaaggaatc atatcggcta atcggtattt tggagtattt atataataaa
     1261 accaaaggtg aaaatgcctc tgaaaatctt aatttcggat tatatggaat attacgcttg
     1321 aaaaatcatt tgaatttaat cgcctacctg aaactggata gtgaatggca aagaaaggat
     1381 gattttttag agtttgacca cagagataat aagctcggaa ctatcctttc cgtgagatta
     1441 tagttgacat aatatttcca gattttgata tggaatcatt aaagaaaagg aggaattttt
     1501 ataatgaaag tgctaaagac ctttcctgat aaatgcataa cctgccataa ttgcgaaagc
     1561 gcctgctcca aactctattt taaagaggat aatgcagaaa aatcgtgcat agtaattaac
     1621 gaagaggttt atcctccgga aatgattgtc tgtaatcaat gcgggaattg tattgatata
     1681 tgtcctactt tagcattaac tattaatact caaggggtag ttatgttgaa taaatccctt
     1741 tgtattggct gtttaatgtg cgtagctgcc tgtccgaata atgcaatgcg ttttgctaaa
     1801 ggtgtttata atccttttaa atgtattgct tgcggaagtt gccccaaaac ctgtcccgct
     1861 gaagcaatag aattagtaac cgcttaagga gagaaaaatg aaccgtaaat taatttccga
     1921 attcaaatat gacctggcac ctgttattcg tggctataat aaacgctcat tgtatataaa
     1981 tttaagcact aatgaaataa aacaaaagcc agttaccgag ctaatgattg ataaatttgt
     2041 tggcgggaaa ggttttgacc tttatctgat gtggcatagt gttcaagatg ataccaaatg
     2101 ggatagtccc gaaaatgaaa tctgcatttc tttcggtccg ctttgtggaa atactagtta
     2161 tccaggtagc ggaaaatcca ttgttacaac tatttcgcct ttaacgggaa ttcctgtgga
     2221 ctgcaatgta ggtggtcatt ttggtcctta tgccaaattt tccggctggg atgctattga
     2281 acttcaaggg attgccaatg aggaagtgat aatttatata gatggtgata aaggcattgt
     2341 gcaaattctt tccgctcctg aggaagagat taatagtcat attcttgctg aagaattgat
     2401 taaagaattt gccgacggac cagataaata tcaatttgtt tctgtggttt ccagcggcaa
     2461 agcggcagaa aatgttttaa tcacttgtct gaatttttcc ttttgggata aaagaagaaa
     2521 agtggctcgt ttgaaacagg caggtagagg tggaacaggt agtgtattca gacacaaaaa
     2581 aataaaggca ttggtggtta aatattccgg tctgaaagtg gattctaata atcccgagga
     2641 tgctgaaacc cttagaatga ctggcttgaa actgcataaa gaaattgaag ctggagatgc
     2701 cactcaaaat cggatgcgcc aagtgggaac tgcacatttg atggaaatta tgaatgatta
     2761 cgatttactg cctatccgca attttaaata tggtcaaagc ccggaagcgg aaggtttaca
     2821 ttcccgcgaa tttaagaatc tatttactca aggtatgcca gatggttgtt ggtatggttg
     2881 ttctctttcc tgttgtaaag gtgtggataa ttttgaacca agaaccggac cctacaaagg
     2941 tcaaaaggtc tgtgtggacg gtcctgaata tgaaacagct gcgggttgcg gaagtaatat
     3001 cggtgttttc aatgcccggg atgtagtaga aattaatttt tactgtgata cttatggagt
     3061 ggatacaatt tcatttggaa ccagcactgc ttttgcaatg gaatgttatg aaaacggtat
     3121 tttgaataaa gaacgcactg gcggacttga acttacttgg ggaaatgcag atgctgcctt
     3181 ggaactttta catcaaatgg ctaagggaga aggttttgga gttatagtag gacaaggtgt
     3241 gcgcagaatg aagcagattt ttgcagaaaa atatggtgcc gatcccaatt tcctgcaaga
     3301 cattggtatg gaggcaaagg gcttggaata ttcacaatat atgagtaaag agtccttagc
     3361 tcaacaaggt ggttatactc ttgccttaaa gggacctcaa catgatgagg catggctaat
     3421 ttttatggat atggtgaata accagattcc tacctttaag gataaagctg aggcattaca
     3481 ttattttccg atgtttagaa cctggtttgg tttacaggga ttatgtaaac ttccctggaa
     3541 tgatattgaa ccggcaaata atgctgaaac cgatgaacca gcaaaagttc cggaacatgt
     3601 tcaaaactat gttgatatct ataaagctat aacgggtaaa ccactggata aagtttctct
     3661 gattgaacag tcagaaaggg tctataattt ccagaaggtc ttttgtttac ggatgggaaa
     3721 aggaagaaga attgatgatg ttccacccta tagagctgtg ggtcctgtaa ctgaagaaga
     3781 atatctttcc cgtcaggaac gctatgatca gcagttgaaa gaaaaaatag gtgttgaccc
     3841 tgaagggaaa agcactgctg aaaagatgga tattttaaga aaatatagag aagaccaata
     3901 tcagcaattg attgatgctg tttatgaaag aaagggttgg acaaaagaag gagttcctaa
     3961 aattgaacat ttgcaaaaaa taggaatgga cctacctgag gttattgaag tagtgaaaag
     4021 atttctctaa tatatgctca aatagataaa acaggttgca cagatatttg gaaatgtgtc
     4081 gtcataaatc ataagcgcgg tcaaagttat ctccaggcaa aaaatttatt aaagcgcaaa
     4141 tgactcctgt cttacattct caattgtcaa ttaattctct ttatctctct gtgaaacaat
     4201 ccgttattcc ttttatgcga aagttatctt aacgcaccat atagagcttt gatatctttt
     4261 actttaacaa cttttggatt ggcttttact ttagcgtaac tggaaatgaa gattttctca
     4321 taacccaatt tcagagtttg gctgatatgt ttatccaatt gtgaaacagg acgaacttct
     4381 ccatttaagc caacttcccc gataaatacg gctttttcaa ttagtggttg atctttcata
     4441 ctggaaatta gagcagctaa aatagccaaa tccaaggaag gatcggtact gcgtattcca
     4501 cctgtcaaat ttacaaagac atcacaattg cgaagataaa gtgagaggtt tttttccaag
     4561 atagctaaaa gcatagctaa ttttttttgg tctagtccta ccgctactct ttgcggagtt
     4621 ccgtaattgg aagcatttac taatgtttgc acttccacaa tgaaactgcg gctgccttcc
     4681 ataatacaac taatagcagt tccgatgtca ttttgttcgt ggctgagaaa gatattattg
     4741 ggattggaga cctctatcaa accgagatgg gtcatttcaa aaagacctat ttcattggta
     4801 gaaccaaaac gattttttac tgctcgcaga attttgtatt gactgcgttg ttctccttca
     4861 aaatagagaa cggtatctac catatgttca ataattttgg gtcccgcaac taatccttct
     4921 ttggttacat gacctactaa aaatagaggt atacctaagt ttttaaccgt tcgtaataag
     4981 cgactggtag tttctcttaa ttgagtaaca gttccaggaa gggcatcaag tgaaggaatg
     5041 cttaccgatt gaatagaatc cacaattacc agatcaggtt tattttcttc aattgcttca
     5101 ataatgtgtt ccgcattgtt agtgcatagc agataaatat tttcacttgt aactttcaaa
     5161 cgtttactgc gtaagcgaat ttgttcggta ctttcttcac ccgaacaata gagtgttttt
     5221 ttattctgtt tacccatcca ttcagaaagt tgcagcataa gagtagattt accaattccg
     5281 ggttcaccgc cgatcaaaat cagcatacct tttacaattc ctccacccag aaccaggtca
     5341 aattctttta ttccggtaag taagcgagaa aattcatcat attccagatc aattattctt
     5401 tctggtttac ttagaggtaa tgtttctacg ggtttattat tttttcccgt tacgcgagta
     5461 gattctttta aagtgcccca agctccgcag gcagggcatt ttccactcca tttaccggtt
     5521 tcaaaaccgc aatcagtgca gaagaaaata gttgccataa aatcaacctc aaattttttg
     5581 ccataataat ctgatgttat tttatggcaa gaaaattaag catcttgctg gatagttatc
     5641 tataatagca ttttaggggt taagtttttt taccgttatt caagcgtaat ttttaatcgg
     5701 caaaaatatt ttttgctttg gggaaatttt gcgttataat gtgaaatagg gagattaaaa
     5761 tgtctgcaaa agtaattgta ttttccactc ctacttgtat ctggtgcaag aaagcgaagg
     5821 agtatctaaa atccataaac cagaatttta cagacataga tgtttccaag gatattgctg
     5881 ctcggaatga tatgatccgt aaaagcggac aggaagctgt tccgcaaata tggataaata
     5941 atattccagt agtcggtttt gatctggaaa agatcaatcg tttattaaac ttaaataata
     6001 ctaaaaaagg agaatcaaat ggctgattat gcaaaaaaat tccacgagct catcactcgc
     6061 ctgcaaatgg ttttgagtga aattgactat gctcaaaaag catgcttaca agcaggaaga
     6121 atggagtgta tgctgttaaa ctacctttat aaggtaaacg gtcctgccaa tatgaacgaa
     6181 ctggctaagg aactgaatgt ttctcatagc cgaattacca gaattatgga taaccttgtt
     6241 gccaaacaac ttgttattcg tgaaccttcg gaagaagacc gtcgttgctg gtttgcccgg
     6301 attactgaaa aaggtaagaa actggcagcc aacagcttac agacagtttt agatcaacag
     6361 gcaaaaatac tgaagaaaat acctgaaaag gatattgagg caatctataa ggcacttaaa
     6421 ctctatgtgg ataaatatga agaggtattg aaagaaacaa tagcaggatt ataatgtcaa
     6481 cttatgtaaa aaccgtttgg tctggtggga tgagctttac agctgaagtc aacggacata
     6541 agctgattat ggatgctgat ccctattttg gtggtactga tcaaggtccg aggcctaaac
     6601 ctttattgct tgccgcctta agtggttgta gcggaatgga tattgtttcc attctggaaa
     6661 aaatgggaat aaaggattat aatttggaaa tggatgctcg gggtgaatct gctactgagc
     6721 atcctattat ttatcatacg ataaccttaa cttacaactt tcaaggcaat aaccttccag
     6781 ctgaaaaaat agtaaaagca gtaaaacttt ccctggaaaa atactgcgct gtgaatgcta
     6841 tgttgaaaaa agctgcgaaa ataattccca aaatctatat taacaatttg gaggttccat
     6901 tatgaaatgg tggcttctca ttatactgtc tttgtcttta gcatttttag gttgtgaaaa
     6961 aaccgatcaa cctacaataa atgaagatgg aacaaaagat aacggaaaat tagagtccgg
     7021 aggaaaaaat cctgaatcta aaaccggtgt gtggcttact gattggaatg atgcaatgaa
     7081 aacagctgat gtgctgaatc gtcccgtttt agttgatttt acaggttctg actggtgtat
     7141 ctggtgtaaa aaattggagg aagaggtctt cggtaaaggt gaatttttag agtatgccaa
     7201 aaaaaatctc gttttgctga aagttgattt tccccgtgat attgagcaaa gtgaagaatt
     7261 gcaaaaattt aatgaacaaa aaatgaaaaa atacgggatt caaggttttc ccaccattgt
     7321 tctcgtaaat tctaaaggtg aagaaattgc tcgcacgggg tatagacccg ggggagcaaa
     7381 agaatatgta aaacacttac aggaattgct acagccaaaa gaataaataa ttacctgctt
     7441 aataacaaaa tctctttcaa tattggaggg agacaaaaat actctggtga taagatactc
     7501 caaacagtaa gcacaggata atatgatgtc cccgtctttt ttttattggg aatttttaat
     7561 gcgtagccgg aggatgatgt tcttcggcaa aaaaacgcgg agcttcaacg agctccggct
     7621 atacaataac ttgtgacaac tttaaaatta gcaacacgga tttaacggat ataacggata
     7681 aacgcaaatt aaatatttgt aatcacacct gcactaaaaa ttcctccaaa atagcttaac
     7741 ttttaaagta tcaataaatt atccctttct tttgtctcct acaataaaat gtgagtaatt
     7801 gcttgtaaca tagtagatta tctaacgact ccgtaacact tccgtaacac tgcagtagtt
     7861 tcgttacgga gttgttactt ggttgttact tcagtaatat gcagtcaact aagtatgaac
     7921 cttatatttt tgaagccaaa ctccttgtgc aaatctcaca gattacgctc ttactatttt
     7981 cctacattcc agcttgacaa atatagccat cctccttttt ctgcatagat aaagtttaaa
     8041 gagaggaata aagatgaata gatacatact gaaaggtggc acaattctaa catttgatag
     8101 tagccatccg gttttagagc aggcagaaat tttaattgat gatggttata tcctgcaaat
     8161 cggttcagaa aaaagctttc ccgatgaggt ttgtgaacgc atagatgtag aaggcaaaat
     8221 cattatgccc gggctcataa atgctcatca tcatttctat tctacttttg taaccggctt
     8281 gggaaaagct gctcctgcca agaattttaa tgctgtgctg gaaaatttgt ggtggcgttt
     8341 ggataaaaaa ctgctttctg aagataacta ttatagcgcc ttaatttcta tccttacttc
     8401 cattcggaaa ggaacaacca ctattattga tcatcatgct tccccttttg ccgtgaaagg
     8461 ttctctttct caaattgcca atgcggttca agaaacggga ataaaagcat ctttatgcta
     8521 tgaggtttca gatagagacg gagataaaat tgcccaagaa ggaattgagg aaaactataa
     8581 ctggataaaa aattgtcaaa acaatcccaa tccttattta aaaggacttt ttggattaca
     8641 ttccgctttt acgctttcgg acaaaaccct atctattgta gctgataagg ttcgggaact
     8701 taactgcggt attcatattc atactgcgga agccgaagca gatgaaaaat acaatctgga
     8761 gcattatggt aaaagagttg tggaacgctt gagcgatttt aatttgttga ataataaaag
     8821 catcctggct cattgtgtcc atttaaaccc caaggaattg atcctggtgg cggaaaaagg
     8881 cgctgcaatt gttactaatc cgcaatctaa tttgaataat gctgtgggca ttgccgatgt
     8941 ttgtaaaatg acggaattgg gcgtaactgt cggtattggc acagatgcaa tgaccaataa
     9001 tatgctggaa gaagtcagag ttggtatttg ggctcaacac cttaaacaga atgatccctc
     9061 gcgtggcttt gttgaacttg cctctgcttt ggtttttaat aatccgctaa ttgcgcaaaa
     9121 atattggggg gcaaaatttg gaacaataac tgcaggcagt cctgcagata ttattgtggt
     9181 tgattacatt ccgcatactc cttttaataa agaaacatgg ctgggacatt tgatttacgg
     9241 aatttcccag gcaaatgtgg aagcaactat ttgtcagggc aaaatcctga tgtggaacgg
     9301 agaattgctg ctggatattg atgaacagga agttaaagcc aaagcaagag aattagcgga
     9361 aaaattgtgg gacagatttt gaaaagcgaa ataaattttt ttacaatgag caaaagagaa
     9421 gaaattagaa tgaagaatgt ccaaaaaggg aaaataacgc tgttggaatt ggaaataacc
     9481 ctcgggataa aaaataaatt tgcagaatcg gcgttaaatt ttttaccgta aaaacataag
     9541 gagattgata atgtatagag agatttttgc aagggcaaaa cattatgaag ataccagcac
     9601 ccgttgttta atggatatgg tgaaaatacc ttctttctct actaaagaaa aagaagttgc
     9661 agaatgcatc ctgaaagaaa tgcaggaaat aggaatggat gaggcatata ttgatccttt
     9721 gggtaatgtt atcggtcgtt tgggttttgg caaaaaagtg attgcttttg atgcgcatat
     9781 tgatactgtt tatccgggag acctttctct ttgggatttt gatccttttg ctgctcacat
     9841 caaagacgga aaaatttggg gtagaggaac tgttgatcag aaaagcggaa tggcaagtat
     9901 gcttacttct gcccgcataa tgaaagattt ggatttggga aaggaattta gcatttattt
     9961 tacaggaaca gttatggaag aggactgcga tggcttatgc tggcagtata ttcttcaaga
    10021 agaagaaatt aaacccgaac ttgttgtaat tacagaaccg acaaacctga atatttatcg
    10081 gggtcataga ggtagaatgg aaatggaggt tagcattaaa ggactttcct gtcatggttc
    10141 tgctcctgaa cgcggagata atgccattta taaaatttcc cgtatcgctt tagaaataga
    10201 gaaattgcat caaaatttgc gttatgacga ttttttggga aaaggaagta tttgcgtaac
    10261 tcaggtcttt tttacaggac caagccaatg tgctgttccc gatagtgcac gcattcattt
    10321 agacagacgt ttaacctttg gagaaaataa agaaagcgct gttgccgaag ttcaggatgc
    10381 ctgtaaaaaa gccggttatc ctgatgctac aatagaaatt ttaacttatc aggaggcagc
    10441 atatacagga tttgtttatc ctacagaaaa atattatcct acttgggtta cacctttgga
    10501 ttcagtttat gtgcaaaatg ccgctgatgc ttacacaaaa accctgggaa caaagccact
    10561 tattgataaa tggacttttt ccactaatgg tgtttctatt gcaggaatga aaggaattcc
    10621 ctgtattgga ttaggtcccg gtaatgaaat ttatgctcat tcacctaatg aagcatgccc
    10681 gattgaacat ttaactaaag ctgctgcttt ttatgctgcg ctggtttata gattttcaca
    10741 ggaaaagtga aatggatttt ttcacaaaga gaaaaagaga gaaagagaag aattgacaat
    10801 tgaaaattat aaagaagaat ctgtgttaat cggaataaat cctggtgcta ttcttttatc
    10861 tgcgaaatta gcgaaatcta tgcgagaaat agatgaagag ttatttaact aaataccgtt
    10921 gtattgaatg cgggaaagaa tttgacccta atgaactgca ttatctttgt gatgaatgcg
    10981 gccgggatta tctacccgga atgccgttaa aaggagtttt ggaagcggtt tttaactatg
    11041 aagaaattgg cagaaaatgg aaacaaaatc ccgaccctct tcttttcagt gctgtaaatc
    11101 ctaaatttta tcctcctttg cctgtaggga acacaccttt ttttagggtt aatattcctg
    11161 gtttgaaaag cggaggaacg gcatcctcca gctacacaaa atggtggata aaatttgacg
    11221 gtttaaatcc tagcggcagt tataaagatc gtgcctcaca acttgttgta gctgatgctt
    11281 tacaaaaagg gattaaagaa attgtaactg ccagcacagg aaatgctgct tgttcacttg
    11341 cctgtttagg tgcttcagcc ggtttgaaag ttgttatttt tgctcctcaa aatgctcctc
    11401 ctgcaaaatt aattcaaatt caggtgcata atgcggaact tcataagatt gatggaagtt
    11461 atgatgatgc tttcaaagcc gcgttggaat atacaaaaac gcataattgt atgtgtcgga
    11521 atacgggcta tcatccttta accatagatg gcaaaaaaag tgccggaatt gaaatttatg
    11581 tccaaaatgg atataaagtt ccggattgga ttgtgattcc ggttggcgat ggagttattt
    11641 taacaggtat ctacaaagca tttttggact taaaaaaagc cagaataaca gaaaaaatgc
    11701 ctcatctctt atgtgttcag gcagaaagca gtgatgccat tacttcctat tgggaaacag
    11761 gaatttatca cgatgctgaa aaccctatta ctatagccga ttccattaaa gtgaagacac
    11821 ctgctttagc gcattggtct gttaaagcac tcaaagaaac agatggaaaa tgcatcaggg
    11881 taagcgataa agaaattcag gaagctcagc tggaactggc aaaagaaaca ggtgtttttg
    11941 gtgaaccctc ttcttcagca actttggctg gactaaaaaa ggtagtaaat catagttggt
    12001 taaaagatgc taaagatatt gtattattga taacaggtca cggtttgaaa gaccctggag
    12061 ctgtaaaact atgaaagcaa gcggaattta cgatttccat gtgcatattg gtgaaaccat
    12121 tggtggacac cttttagcag atagttggaa aagtttcaat cttctttatg aacataacgg
    12181 tttggaagga attggtgttt ttgtaaccga aagccagagt gaaccgcttt cttccaaact
    12241 ggcaagaatg cgaaaagcgt cccgcagctt tttgggaaag gttttctggc atttaactcc
    12301 ccggcagttg gatgttcgca gattagaaaa cattcttaag gaagataccg atttgaaact
    12361 ctatactacc tatcgtgagg cagggctata tcaaagctat gaaagtattg aacacttgat
    12421 ggaagaaatg ccctccccta aaaaaagatt gctggtgcac tgtgaagatg atgaaattat
    12481 tagtgaatat agtgaacgct atccttttca taatccttcg gatcactgtt tgcggcgtcc
    12541 tgaaaaggcg gaaatttctg cagtggaaaa gttacttgat ctttctataa aacatcatta
    12601 tcctcttcac atagtgcata tttcttctcc tcagtcagcg cttttagttc aacaggcaaa
    12661 aaaagaagcg gattatatta cttgtgaaac tgcacctcac tatttgcttt tgaatgaaga
    12721 ggtcttaaat gaaccggaag gtcatcgttg gctttgttct cctccattac gtagtgaaag
    12781 cagtagaggt cttttactgg aacttttaca ggatggtgtt tttgatatta ttgccagtga
    12841 tcattgtgct tttagttctg aaaccaaaga tgtaggaatt ttgtatcctg ccaaaacccc
    12901 aatgggcatt gcaggaagcg gagttctttt tactttgctg gcagagcatt tggtagaaaa
    12961 aggcaagctt agtatggagc aactttttaa ttttataagt tttaatccgg ctaaactgat
    13021 gggtttttcg gttcaaaatg ataagcttag ttatgaacgc ttgggagctc caattcccgt
    13081 tattcccagt tgggcaaata cacctaatcc gtggattgat ttttggagct attatgaatt
    13141 gaggaggcat cataatgatg tttactgatg catttcgtaa tgacgctgct gctaaagtaa
    13201 caggcagagc aaaatatact gatgattata aacttcctga tatgctttat gctgttcctt
    13261 tgcattctca agtggcaagt gctataatta aatcaattga ttactctgaa gcgcttaagc
    13321 aaaaaggagt ggtagctgtt tatacggcaa aggatatccc cgggaagatc aaatttggcc
    13381 agattattaa agattgtcac actataatta atagaaggat tcgttctgcg ggagatgtta
    13441 ttgccttagt tgtggcagaa actcgggaaa ttgctctttc ggctttgcct ttaattaaag
    13501 tggaactgga agaaattccc gctgtttata atccagaaga ggcaatgcaa gaaaaaagcc
    13561 ctttggttaa tcctgatttt ggtagtaata tctgcaacta tcataaagtg cgaactggtg
    13621 atattgaaca gggtgaaaaa gaagcagata ttattatgga acaggaattt aatacttcac
    13681 gcgtagagca cacttatctg gaaccggaaa cagcactttg ttatttacgt ccggatggag
    13741 ttatggaagt tcttggcagt atgcaacatc ctttttctac aagaagattt gttgcttcca
    13801 ctttgggttg taaactaagc gaagtggaag tgaaaaatat tcctatggga ggcggttttg
    13861 gaggaaaaga tgatacagcg gctttggttt gtgccagagc agctttatgt gcttacctaa
    13921 caaagagacc tgtaaaatta acttacagcc gggaaatgag tatgcgggaa agctataaac
    13981 ggcatcctta taaactgcaa tatagaatgg gactgaaaaa gaacggcaaa atcgtcttta
    14041 tcaaagctcg tattattgca gacggtggtg cctattgtag tgtaacgcct tgggtaactt
    14101 ggcgttccac agttcaaagt tgtggctgct atgaagttcc caatgtgcag agtgatgttt
    14161 atggtgttta taccaacaac attttttctg gagctttcag aggtttcggt tctccgcagg
    14221 taaattttgc catagaacag ttaatagaaa tggctgctca aaaattgaat atggatgaaa
    14281 cagaattccg aaagataaat atggttcatc aaggcagcaa gacaattacc gatcagaaac
    14341 tggataatca taccgtttct ttgcaggagg ttatggatag cgttttaaaa gaaattgatt
    14401 acgaaaataa agtaaagaaa tgcagtttcg gcaatcctaa gatagatgaa tggtatggca
    14461 tcggttatgc aatttcttat cggggagtta gtttaggagc agaaggaacc gatatgaaca
    14521 gcgctattat ttatattcaa cccgatggca gtgttttact ggaaacggga gttcacgaaa
    14581 acggtcaggg agctcaaagt gcaatgatct taattgcatc agaagaactt ggcatcccta
    14641 aagagcttat tcgttatcgt ttaccttcca cttccaatat tcctgatggt ggttctacag
    14701 ttgcttccag aggaactatt atggggggag gagctgttgt taatgctgca aaaataatta
    14761 aacagcgtct ctgtgaggga gtagaaaaag ctcttggcat aaaggattgc agttataaag
    14821 atggtaatgt ctatttcgtt cagggagaaa aagttgtgga ttactttgaa gcagttcgtt
    14881 tatgttatga tcatcaaatt tatccctatg ccttcggagt ttttcaggca ccgccaatta
    14941 gctgggatga agaaacaggt catggaaacg cttatttttc ctgggtttac agttgccagg
    15001 cagcggaact aaaagtgaat tccaaaacag ggaaaatcac tttactgaat ttagttgccg
    15061 ctcacgatat tggcagagct atcaatcctg ctttactggc tggtcagatt tatggtggaa
    15121 tggcaatggg agcgggttat gccctatacg aaaatttccc cttggtaaat ggcgaaccaa
    15181 aggcaagcaa ttttaacaac tatagagtga tgcgaagtac cgatttaccg gaaatgacag
    15241 ctatttttgt ggaaaatccc gatcccaatt caccttccaa ggcaaaagga attggagaac
    15301 cggctttgga aattaccgct ccagcaattg ccaatgccct ttacagagct accggaaaaa
    15361 gatttacaga cctacctatg gctccgcagg tttgggaata tgttaaagga aagcaaaaat
    15421 gaaaataact gtaaataaca ttgaatatac tgtgtccgaa aaccttgccg aactgaaact
    15481 ttccgcctat ttgagggaaa atctggattt aacgggaacc aaaatcggct gtgatattgg
    15541 tgtttgtgga agttgcacaa ttctggtaaa taataaacca atgcgttcct gcctgctgaa
    15601 attaaaagat gttgccgata aacaggtctt aacgattgag ggactggcat cttccgatgg
    15661 aactttacat cctattcagc aggcatttat tgatgcagga gcaattcaat gcggattttg
    15721 caatccagga atggttttaa ccgcttatgc ctttttgctg gaaaataaac agcccacgcg
    15781 ggaagaagcc agagaagcaa ttaaaggaaa tatttgcaga tgcacaggat atcagcaaat
    15841 tatagatgct atcctcctgg ctgcacaccg tatgcaaaaa agattagaca ccgagaacac
    15901 cgaaatattt gataatttaa cccgtatttt gtaataatgg gatacctgag atgttcatta
    15961 caagtttcac ttttacactt tttcaccttt tcactttcag tttttataca aaatataaaa
    16021 tagaggtata atatggagaa gattaatacc ctgattaatg aactggaaac cttaaaaccg
    16081 gatttattcg gtaaggattt ccttttaacc tgggataaca gtttggacag cttgaaagca
    16141 gtaatgctgg ttgctgaaat cctgcagaac ttgcattggc aaaagaagcc ctggcgaatt
    16201 tttgattatg gcttagcaat atctattttt cgggataaca gcacgcgaac gcgtttcagt
    16261 tttgcttcgg cagtgaatgg tttgggcttg gctttagcgg aattggatga agtaaaaagt
    16321 caaattgcgc atggtgaaac ggttcgggaa acagccaata tgatttcctt tttaactgaa
    16381 gtaatcggta ttcgtgatga tatgtatccc ggagaggggc atacttatat gttggaagtt
    16441 gccaatgcta ttagcgaagg ttatcagcag ggaattttag cacaaagacc tgccgttata
    16501 aatttacaat gcgatttaga ccatcctacg caagttcttt ccgatctttt gaaactgaag
    16561 gattattttg gcggatggga aaatctaaaa ggcaagaaaa tagctatgag ttgggcttat
    16621 tctccttctt acggaaaacc gctttctgtt ccgcagggaa taataaatct gatgacccgt
    16681 ttcgggatga atgttgtttt ggctcatcct gagggctatg atttgcttcc ggagaccata
    16741 caatcggctt tttcatttgc tcaagagagc ggaggttcat ttagacaaac cactttaatg
    16801 gaagaagcat tccaagatgc cgatatcgtt tatcctaaaa gttgggcacc aatgtcagtt
    16861 atgcaacaaa gaaccaaatt gcttaaagct ggtgataaac agggcttgaa agaactggaa
    16921 ctgcattgtt tggctcaaaa tgaaaagtat attgactggg aatgcgacga cgagatgatg
    16981 aaattgacca aaaacggaaa tgcgctttat gaacactgtc ttcctgcaga tattagcgat
    17041 gtaagttgtg aaaatggtga agtggcaaaa agcgtgtttg aaaaataccg tttgcatacc
    17101 tatcaggaag cgggttataa gccatttgtg attgccgcga tgatttttat gagcaaagtg
    17161 aaagagccag cagcaaagct gagaagtttt gtgtaacaaa gagaaaagtg agttggtagg
    17221 gtagccagtt tgcgagatgg aattggaaaa aatgtattat tttgctgttt tccaaagaga
    17281 gaaagagatt ggatttacac agaagataag gttatatcat ttttttatgg gcatatcatt
    17341 caaaaagagg ggtcagttca ttttacagga aaaacaattg acaaggcagg gcagaaaaaa
    17401 aacaaggtaa taacataaag taaaaaaata acggagttag aagatgaaaa acattctggt
    17461 tctcggagcg gcaggacaaa ttggttcgga acttgttcct tatttgcgta agctctatgg
    17521 ttcaaatgca gttgttgctg cctatcgtag cacaccttta acaatggaaa ttcaggaagg
    17581 tggtccctgt gaaaatttgg atgctatgga agcggataag atgtttgaag tagttaaaaa
    17641 atacagtatt gatactataa tcaatctggt agctgttctt tccgccaaag gggaagccaa
    17701 tcctttaaat gcatggaata taaatatggg aagcttgatc aattccctgg aaataatgcg
    17761 tgaagttaaa ggtgctgttt ttacaccttc ttccatagga gcatttggtc cttccacacc
    17821 tttggataat accccgcagg atacaattat gcgtcctact acaatttatg gcatttccaa
    17881 agttgcagcc gaactttgga gcgactatta tttcttaaaa tatggagtgg atacgcgggg
    17941 agttcgttat ccgggaatta tttccaatgt tactcttcct ggaggaggga cgactgatta
    18001 tgcggtagag atttattatg aggcaattaa aaataaacat tatacctgtt atctgaagcc
    18061 gggaacttat ctggatatga tgtatatgcc ggatgctatg cgtgcctgtg tagagcttat
    18121 ggaagcagac cccaaaaaat taaagcaccg taattgtttt aatgttacag caatgagcat
    18181 tgaacccgaa atgattgcca aggaaattag aaagcatatt cccgaattca caattgacta
    18241 tcaaattgat cctgtaagac aatccatagc agaaacctgg cccaattcta tggaccagac
    18301 agcagctatg gaagaatggg attggaaacc ggaatatgac cttcctgcaa tgactttaga
    18361 tatgctgaag gttctttccc aaaagcttaa gaaatgataa aaattggagt tgtgggagtt
    18421 ggccatttag gtcaacatca tgcccaaaag tttcaaaaca ttgatggtgc ggaattagtt
    18481 ggcatttatg acatcaatga acaacgcgca aaggaaatag ctaaaaagct aaataccagg
    18541 tcttttaaca gctatgaaga gcttttaact gcctgcgatg caatagatat atctgctaca
    18601 accacagctc attatgaact tgccaaaact gcattacttg ccggaaagca tattttcgta
    18661 gaaaagccga ttacgagtga attagctcaa gcgaacgact tgcttaagct tgcttccgaa
    18721 aggaatctca ttattcaagc ggggcatatt gaaaggttca atcccgttat cctgaaaata
    18781 gagaatgaaa tcaataatcc tctgtttatt gaatcgcaga ggatttccac ttttcagccg
    18841 cgaggaacgg atgttcctgt tgttttggat ttaatgattc acgatataga cctcatttta
    18901 agctttgtgc atagcccggt taccgatatt aaagccagtg gagtaggtat ctttacttct
    18961 acgatagata ttgctaatgc gcgtattgaa tttgccaatg gagctgttgc caatgtaact
    19021 tcttcccgcg tttccacaaa acaggaacgc aaattaagat tttttcagaa tgatgcctat
    19081 atcaggatgg attttctggc aaaaaatgtg cgtgttatca aaaaaagttc cgatataaac
    19141 agtttattac ctcaaattct gatgggagct ccaaatatca atccggagca gcttttggat
    19201 attaaaagct atgatgccga cgatattaac aaggatgctt taactttgga actggagtcc
    19261 tttattgatt gtcttaaaaa caataaaaaa ccggttgtag atggagaagc aggaaccaga
    19321 gctttggaaa tagccactaa aattataaat caaattcaaa cccaaaatac cagaataaac
    19381 ttattacaca ggtaaaagaa aatgcaagat gtatttgtaa aagacataga aaagtattta
    19441 ggacaggaag ttcggcttaa gggctgggtt cgcaatattc gtcatagcgg aaaactgctg
    19501 ttcataattt taagagatgg aacaggagaa attcaaaccg ttgcctttca acctgatttg
    19561 ggagaagaag catttgaaac agcaaaacat ctcacccttg aatcgtccgt aataatttgc
    19621 ggcattccta agctccatcc taaacaggaa ggggtttatg aactgcaagt tacgcaagtt
    19681 cagcccctgc aaattgcgga tgaatatcct attggtaaga aagaacacgg acccgatttt
    19741 cttctttcca acaggcatct ttggatacgc tctcaaaagc aatgggctct tttacggatt
    19801 cggcatactg tttattatgc tatttgcaat tacttaaatg agcataattt tattcgtttt
    19861 gattctccta ttctaactcc taatgcttgt gaaggcacta ccacactttt tgaactggat
    19921 tattttgacg aaggcaaagc atacctttcg caatcggggc agctctatct ggaaacagga
    19981 attatgagtt tcggcagggt ctatgatttt ggtccggtat tcagagcaga acgttccaaa
    20041 acccgtaagc atttaaccga gttttggatg atggatgccg aagcagcttt tgtggaacac
    20101 gatgagaata tgcaaattca ggaagacctt attcgctatg ttatccaaac tgttctctcc
    20161 gaatgtgccc tggaactgca aattttggaa cgaaatacgg aggcattaaa agcggcggat
    20221 gctcctttta agcggatgac tcattatgaa gcagtagaat tgttacgcca aaaagggagt
    20281 gatattactc acgataaaga tttgggagcg caggatgagg tcttgctaac ggaggattcg
    20341 gaggttcctg tttttgtaga acgctggccc aaagaaatta aagcatttta tatgaaacgc
    20401 gatcctttaa atccgaattt ggttttagga gatgatttaa tagctcctga gggctttgga
    20461 gaattgattg gaggcagcca gcgtgaggat gattatgagc ttttactttc cagaatgcaa
    20521 gcggaaaata tgcctataga tgattatcaa tggtttctgg acttgcgaaa atacggttct
    20581 gttccgcata gcggttttgg cgttggattg gaacgattgg ttgcttggtt gagtggaact
    20641 cctcatatca gagagaccat accttttccc cgtatgattt accggattta tccctgagta
    20701 acaggatttc tgctttcgca ggaagggcaa aggtcttttc ctgcaagaaa gaatattagt
    20761 ttcaaaaata aaaatataag gatagaagaa tgattacgaa ttacttttcg gaaactacga
    20821 aaacaatgaa atcatcgctc atcagagaac tggtagcaac cactaaaaat gttcctgatt
    20881 taatctcttt tgctggaggt tttccttctc cggcaacttt tccgacggat attttgagca
    20941 aattatttca ggaagttgtt ttccagcagg gcagagatgt tctccaatat ggtaacagtg
    21001 aaggtgacga tcaactgaag gaggaaattt taaagtggga gaattatcct aacctgaatc
    21061 ggaataatct gattattacc gtaggagcca ccaatgccat ttattatatg agcagaactt
    21121 tacttaatgt aggagatgtt gttctttgcg aagcacccag ttttttgggt tctattgttg
    21181 cttttgaagc tgtaggagca gaggttcatt gtgtttctat ggataatgaa ggaattgatt
    21241 taaatcgttt ggcagagaaa gttaaacgcc tgcgcgatgc cgataagaag atcaaatttc
    21301 tctatactat tcccgatttt caaaatccca caggtattac gatgagttat aaacgtcgct
    21361 atgaattgct taaatattgt gaagcggaag atattctcat tatggaagat aatccttatt
    21421 cccgtttaaa tttcagcgaa gaagaactgc ccactttatt acgcatcgct caaacggaat
    21481 taaataatcc ttatctggtg acagaaatcg tttctttttc caaaatattg ggtccgggaa
    21541 tgcgcgttgc ctacgctatt ggagctccgg aattgatcaa taaaatggtt tcctggcaac
    21601 agaaagttac catcagtccc gattgcacaa ccgaaagagt tgtagccaaa tttctggctg
    21661 agggttatat ggaaccgcat attaaaaacc tttgtaaatt ctataaaccc tatctggaca
    21721 agatgttggc ttgcctttct gccgagatgc ctgtttatgt gcaatggaca aaacccaagg
    21781 gcggaatttt tatctggttg tggctacctg aagatattaa tgccgacacc cttttctata
    21841 aagccaaaga ccatttggta accttcattc cgggtagtaa attctatcct gaaggccagg
    21901 aaagatttaa ctgtatgcgt cttaattata cctattctac tatggaacaa atagaaactg
    21961 gcatcaaacg cctgggtgaa ttgataaaat caatttatgt ttgacatcgg tataattatt
    22021 gcttataata tatatagatc ctttgcatca ctaaggagaa aaaaatgaga aaggtcttgc
    22081 tgattactat gctgttagta atgctattac cgttaatagc tcaaagtgat attccgcatc
    22141 caaatttcag tccctatacc aataacggtt cttcgtttct cggtttgaat aaactttcaa
    22201 tgagccattc tatgggtttt caggcaggaa ccagttccac cggtgagggg tattatcttt
    22261 ctctttatac caatcatctt aaatataaat tcaatcccaa actggaaatg aatctggact
    22321 tgaattttgt aaactatggc ggttttgata ccagcagcaa atttgaactg gataatgcta
    22381 attacagtcg tgttattcct gaattttctc tgcgttacac tccccgggat aacattcaaa
    22441 ttcagttaca aatgattagt ggttctattt tgaatcccta taagaacaac tacctgcaaa
    22501 actggtaagg agcattatct gctaactttt gtaattattg ttattgctgt tttgggttgt
    22561 gctttaggca gttttttcaa tgttttgatt taccggattc ccaaaaagca atccataatt
    22621 ttccctggtt ctcattgtga gaactgtaaa agacctattc ctttctatca aaatattccc
    22681 attatcagct atattttact aaagggaaaa tgttccaaat gcggagctaa aattcactgg
    22741 catcatattg tagttgaaat tataacacct cttttgtttt tagtgttgtt tttccgttat
    22801 ggtttgcatg atttccgttt ctataaatac atcctgcttt tttcttttct catacctatc
    22861 ttttttattg atgccttaca tcaaataata cccaatgtgt tgtccattcc gttattgatt
    22921 accggtatca tctttgtttt tatacccgga aacgatgtcg gcatcataaa tggtttagta
    22981 acaggagcag tggttttctg tttgcttcta tttttagctt gggcttatca aaaaatacgc
    23041 ggaaccgaag gattgggtgg gggagatata tggctgttta cagctctggc aacttatttt
    23101 ggtttaacag gcatacctta catctttttg ctttctgcct tgatggggat aatctatttc
    23161 cttattttta tcagagataa aagccaaccc tttgcttttg gcacctttat tgctttagca
    23221 gcggttttct ggtcggtaat tggagaagaa atagttttcc agctaattaa ttttatttaa
    23281 ccgcttagct gttttctctc acagatttca cagataatat ttcccgcaga ttacgcagat
    23341 ttgaataata ggtttaaaga gaaaaggatt atgatgataa caggaatttt attgtgaatg
    23401 atatgtttta ttccttttaa agccaatcag ggcgacacaa tcatagcgat ggtggcgtaa
    23461 gcccctcgta aaatgtaagc cccgacttct tttttctttt catcttccca ttttccaaaa
    23521 gccc
//
LOCUS       DAOPFL010000020        22284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2451_length_22284_cov_92.060147, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000020 DAOPFL010000000
VERSION     DAOPFL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 22284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22284
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2451_length_22284_cov_92.060147"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1133)
                     /locus_tag="PLG69_02775"
     CDS             complement(36..1133)
                     /locus_tag="PLG69_02775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPI42463.1"
                     /translation="MKKRLLIATGGGDCPGLNAVIRAIVKRASFEHNWEVLGSIDAFN
                     GILKDPMHIIELTPEKVAGIHIQGGTIIGTTNKGGPFNWPIKNPDGTWTTVDRSQDFL
                     DRLRYQNIEALINVGGDGSQRISQQLFELGCPVIGVPKTIDNDLSATDFTFGFQTAVD
                     IATDAVDKLVTTAASHHRILILEVMGRFAGWIALHSAIAGGAEVCLIPEIPYNIKNIM
                     QAINERIDRGHDFANIVIAEGARPVDGDMTFVECDEPGAMKIKLGGAGLRLAQELKNA
                     GCTIDIRVTILGHLQRGGTPNAFDRILASQFGVKAFELVLQEKWGSMVAYRHPNIVAV
                     PITEAIEKYNYIDLDSDLVATARGLGISLGD"
     gene            complement(1183..1605)
                     /locus_tag="PLG69_02780"
     CDS             complement(1183..1605)
                     /locus_tag="PLG69_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPI42464.1"
                     /translation="MLSIKRIKELNKKIYEEVSTLWEKTGINKPERKDSFESLQENLQ
                     QTGILIIAEENEKVVGVVWVTNDFRRLYIHHMAVVPNKQNQGIGTKLMEECISLAKEY
                     GYQLKLEVNVDNPAAWHLYEKFGFKELSGYRVLIKRDI"
     gene            complement(1607..2530)
                     /locus_tag="PLG69_02785"
     CDS             complement(1607..2530)
                     /locus_tag="PLG69_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPI42465.1"
                     /translation="MKKSKLILLLVLLILIGLYFVLRTNRPQEKLARVFDLDTLSINR
                     IEIYDAQDTLKMVKQNNIWKLIYPVNWEADSLKIRDLFREVITAKYAKTPMGTGKEAI
                     EKFHQKDEEALHIIVSDGKKSIHTLFSNLDNPYDYFRYAGSDKIYQLKAKVTNNYNTD
                     LSNWRSPHIVSHKEEELLKIEVTHPKNKYTLTRNGLQWTFQDAHNEFKIHPANRAMLK
                     ILNILESLNTYIFIDNAQAEYAEKFKNPYCTVKLYLTGNRTQELKFIEHTQGYYLMMV
                     DNDPSVLFVVVWDTVFRFTRHPDIFRMQEFG"
     gene            complement(2517..4091)
                     /locus_tag="PLG69_02790"
     CDS             complement(2517..4091)
                     /locus_tag="PLG69_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HPI42466.1"
                     /translation="MKKEKKTSSILSNLLIKLAIVLMVLLIGSYARVRWDFSKNKAYS
                     LSQVSKDAVSNLQDNMVVKLYSSKELPAAMLILDRYVKDLLEEYKQAGKGKFHYEVIN
                     GPSPEDLKNKALQYGVGAMYFRIFENDKTTTKEVIYGLVFEYQGNFESMNVLPKMQNQ
                     LEYQMTLKIQKITRYTLPDITAFVDTLYTLMPRQKYENELYSNYNVHFTNLLEPPPKT
                     PVMIVHSGYDSLSITQLYNLDQFLMQGGKLVVLADKIFSDGETVMEIHSNLFDFLENN
                     GIKLSSDIAMDIFCDSRQMGVDTNISFPIYPVLRGSQHPITRNISNIIIYMGNGIIFN
                     RKPGLKFQTILATSTRSALLEGPDYQLDSRLFFNPDPDVFVNPPIPLGAIVEGRMESY
                     FKEKPQFQRSDFVSAVKDGKIVIFGDRELYVDPDKPIYENRYQIILNAVDWLMGREDM
                     INIRSRHLQESILDIAYYMHKNNLLWGDPAKKERRIKMEVKIIATLLPSLILIAVGGI
                     ISLHRKRTKEFKNEEI"
     gene            complement(4093..4815)
                     /locus_tag="PLG69_02795"
     CDS             complement(4093..4815)
                     /locus_tag="PLG69_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI42467.1"
                     /translation="MKTVFTIAKKEYELALHSISTYIVYILFLVIIGIIFSNMVFKIA
                     RAELRFLFEVIHIVFLFYIPAITMGSIAKERQTGTLELLSTLPIKLSSIVWGKILSAL
                     FQIITIIILSLVFFGIIVIFGEGIDYGAIICGYIGLILAGLAYASIGVFASSFPSNQI
                     LAFVLALLISAVFYLLKFLLPLLPFGLVPIVQYFSFDYHLSSFLKGVIDTRDILFFLA
                     VTVIFALLAQFNLQSKNLMQEK"
     gene            complement(4812..5909)
                     /locus_tag="PLG69_02800"
     CDS             complement(4812..5909)
                     /locus_tag="PLG69_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI42468.1"
                     /translation="MIKIDKLSRYFGEIKAVDEISFCIQDKEIVGFLGPNGAGKTTTL
                     RMIVGYLQPSSGNIEIDGESIFTDPLKASRQIGYLPEQNPLYDEMTVVELLAYFAALR
                     KLKRDYFRERLNFVVKNCGLKDVLYQRIGTLSKGYRQRTGLAQAILHDPRILILDEPT
                     SGLDPNQIIEIRELIRELGKEKMVLLSSHIMQEVQALCDRVVIINKGKIIVDDNIENL
                     PNYLKRSTMLILEVQGENVDFSDFLKEYPFVELDILSRNETTCKVCVYETSGGLISSS
                     ITNNESDKNSIDETSEFDLRQELSRFIRDRGWLILEFSTSKMSLESIFHELTIENYYE
                     PEIEEKLSAEQEFMEEEQTKPTETEDNREEQ"
     gene            complement(5911..7074)
                     /locus_tag="PLG69_02805"
     CDS             complement(5911..7074)
                     /locus_tag="PLG69_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPI42469.1"
                     /translation="MRKKWWIIIVIVLLILLVLFLGKSCGKKKQTETVKPTETETYTV
                     KRGDISSRIEITGEVQPEVVVSLKSKVSGKIVKFYAGENDYVTSGQIIADIEPDYNQA
                     NTLFNTKAMLQKAEMNLKNARKDLADKTILLQKQFISQEEYDKASDALKEAEIEYTQA
                     SSQYEMIRDLDVPGKVTHVYATASGVVIERNINEGEMVQSSLTSFGEGTVIMKIADLN
                     KMIVKSNINEVDISKFKLNQSAEITLDALPYEQYNGKVVKIAPQAIIENNAKVFPIEI
                     NLNASGKVVKPGMTANVTILADSRQNVLVIPIRAVFSNDLNQDIVYLVTDTKSSSKET
                     KSKEKTSSAPNSVATPVKLGTNDLLQVEVIEGLKEGDIILLNEPVTNNNKMFM"
     gene            7357..7830
                     /locus_tag="PLG69_02810"
     CDS             7357..7830
                     /locus_tag="PLG69_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPI42470.1"
                     /translation="MNKLFMPELVKIVEKFENKNACLQYMANLLSESGCLSFPDRFLA
                     AVKGREEIMSTGIGKGIAIPHARDLTVSCLRIAVCLVKQDLDFSSVDNLPVHLVFMIA
                     VPQSSNQDYMKILRSLSEFLRREENRETLLQAKDERDLYEKVQFLEEELLPQLGV"
     gene            7778..8749
                     /locus_tag="PLG69_02815"
     CDS             7778..8749
                     /locus_tag="PLG69_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPI42471.1"
                     /translation="MKKFNFWKKNYFLSLAFSILFLMPLLAQHQDAGTTGFSTLKIIY
                     SARANGMGQAMLGRAKNFDGMQFNPASIIRVPNQGVFTTFADHFVGSGGGSVSYVVPK
                     NIYTSYGFYLNYWNSGSIDRTDVGTNGEFIELNDTFNAQDILAGFTLAKFVSPALDAG
                     GGIKFVLDQIDDKSASAVLIDIGILHHTANERIKVGLSAKNLGFQISHYSDKKYDEGI
                     PFTYGAGFGIDVGKNTLLNIDITKANGENFIGKFGVEQQVHPSLVLRGGFKTNAGDYY
                     LGGNMEWTSGISLGVGWVWKKIALDYAVASYGDLGLTNQVSIRYNIE"
     gene            8784..10901
                     /gene="glyS"
                     /locus_tag="PLG69_02820"
     CDS             8784..10901
                     /gene="glyS"
                     /locus_tag="PLG69_02820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPI42472.1"
                     /translation="MERSRFLLELGCEELPERQIEIACNGVSSSFATFLKSNKLYCGS
                     YKVSGTPRRIYLDALELDTHQEDEEILKTGPLVSVAYDEEGNLTPAGKGFLNKSQAKE
                     AAIFIQETEKGKFLAVKYIKKGLATVELLKNWIPEMIVQIPFEKKMAWSQLGLIFSRP
                     LRWFLILWNHKPLELDFGGIPCGNITYGNRFLGLDKSITINKAEDYLPKLLEQKVIAD
                     IEERRKEIITQLAAIFPEGNFKVIEDRELVETVVNLVEYPTAVVGEFEEKYLSLPDKI
                     IISTINQAQKYFAVQDNQGKLSNKFVFISNGNPSASMIIRKGNEKVVKARLADALWYW
                     NEDTKYPLEYWTRHLDKVIFQAKLGTMADKTKRIMQLVSALADELKITEEQKKKAIRC
                     AELCKADLVTNMLGEKEFTKLQGYIGKQYAIASGEDLEVAEGIFEHYLPRGSGDELPS
                     TLCGAIVAIGDKMDTVAGIIGIGMMPTGSGDPFALRRAANGIVQIISSRKWDLDLFSF
                     ADRALELIGKQTELDKKAKKNLHSFLEQRITGLLKTNGIAYDVIESVMHIDKSHLHDL
                     ENRAQALNALKGKEDFIHLVIGFKRVANIIAETQDFVPFNKDKLVEPEEINLYEALQI
                     LHQDIDSALQNKDYPLALQYLIKFGKVIDAFFDAVLVNCEDDELRNNRHSLLKEVKKE
                     FLRVADLSLIVLETEQKEAGKCS"
     gene            10892..11896
                     /gene="pta"
                     /locus_tag="PLG69_02825"
     CDS             10892..11896
                     /gene="pta"
                     /locus_tag="PLG69_02825"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPI42473.1"
                     /translation="MFIIDELINKAKGNSGSIVLPESTDPRVLTAVAKIVAMDLAKVT
                     LLGESEIINQEAKALNIELNNVTIINPETSEDIPAFAEAYYQRRKEKGVTMAQAIETV
                     KNPLYFGASMVKEGKVEGMVAGAANITADVLRAALQVIGVMPGLKTVSSAFIIPVPDF
                     RGEKRIFLFADCAVVPNPSSEQLADIAMSTALTRKAIIGDEPKVALLSFSTLGSAKHE
                     LVDKVTMAKEILTERKVDFDFDGELQVDAAIIPEVAKRKAPQSSVAGNANILIFPDLQ
                     AGNIGYKLAQYIGKVEAIGPIIQGLAAPVSDLSRGCSAEDIVNTVAIVLLLADQQKKK
                     "
     gene            11921..13129
                     /locus_tag="PLG69_02830"
     CDS             11921..13129
                     /locus_tag="PLG69_02830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPI42474.1"
                     /translation="MAIKLSNRCRLIKPSPTLSLSAKAKEMKDAGIDVISFSVGEPDF
                     NTPEYIKASAHKAIDANFTRYTNNAGIPELRQAICEKLLRDNGLKYSPKEILVSPGAK
                     AAIVNSLIAVCDDKDQVLMATPYWVSYPYQVALANAEPVYIPTCEEKGYKIQPEDLEK
                     AIKENPCSKVLIMNSPSNPTGTVYTQAELSAIAEICIKYNILVISDEIYERLVYDEAK
                     HISIASISEEMKQRTIVINGVSKAYAMTGWRLGYAAGPGDIISAGAMVQEHTTSCVNS
                     ITQYACVTALKEEDDSIEKMRVEFSRRRDFLYEELKKLPHITCFKPQGAFYIMPGIKW
                     YLENNNQNIKTSDDFCAKLLDKYYVALVSGNSFGMEGTVRFSYANSIENIKEGLNRFA
                     SFLAELASGR"
     gene            13132..13347
                     /locus_tag="PLG69_02835"
     CDS             13132..13347
                     /locus_tag="PLG69_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42475.1"
                     /translation="MNDFNDKMKERWKDKRKKAYNWPRLIIMVLALIIILYLMNHLGN
                     TKNVVQNPSASVTDTLQADTLQKEITP"
     gene            13344..14237
                     /locus_tag="PLG69_02840"
     CDS             13344..14237
                     /locus_tag="PLG69_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPI42476.1"
                     /translation="MSLVIVGSIGLDSISTPAGEIQNALGGSAIYGALAGSFFTDAYI
                     VGVVGTDFPIDGLNLLQKKGINLDGLELKEGKTFRWSGKYSNWNKVETLSTELNVFAD
                     FYPQLPESCKSCRSLLLANINPSLQMQVLNQVSGYSHLACDTMNYWIYLCPKEIEQVL
                     RRVNIVFMNEDEVREYTNKENIYSAAREILSLGPQWIIVKRGEYGSVAISNDDMYFAP
                     AYPLEEVKDPTGAGDSFAGAFMGYLANHNILNRAVIKEAILYGTVVSALNVADFSVKG
                     LIDKTKAEIDVNKEQLVKWTT"
     gene            14225..15265
                     /gene="purM"
                     /locus_tag="PLG69_02845"
     CDS             14225..15265
                     /gene="purM"
                     /locus_tag="PLG69_02845"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPI42477.1"
                     /translation="MDNLKNGLDYRKAGVDIQAGEVTVLAIKQKVRSTYNSNVLSELG
                     GFGGLYKIDKNLWHQPILVSSTDGVGTKLLIAIMADKYETIGQDLVNHCVNDILVQGA
                     IPQFFLDYIGIGKLEPAKVEKMIEGIIIACQQNSCALIGGEMAEMPDLYEDEEFDLVG
                     TIIGIVEQEQILPRPKIKPGDQLISLPSSGLHTNGYSLVRKIVFEKLKLSLDSYIPEC
                     QTTLGELLLSVHRSYLPLLKDYLTDPNLKGLAHITGGGIIGNLKRILPAGISAQIKLR
                     KTEIPPFFHWLQEAGKLSEETMREAFNLGTGMICIVDNEAFDKYLTIPEARCIGELVN
                     QNPTKEKVEFIQ"
     gene            15469..18507
                     /locus_tag="PLG69_02850"
     CDS             15469..18507
                     /locus_tag="PLG69_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPI42478.1"
                     /translation="MRKYALILLIIMLAGTVFLEAATTGKLAVRVRDNAGRPLEFVNI
                     VVMQGNQRITGGQTNAKGTAIIINIPPGLYTVKFSLIGYDTVVYNDVRIIVDQTTNLA
                     PTMNKSGFEMKAVTVTATEDKVDKNRVGSARNIEMSIVTDAAVSDVADIVALQAGVTN
                     VGGELHIRGGRSNEINYTIDGMSVTDPVDGGATLQVDTDAIKDMKVMTGGFPAEYGNA
                     QSGVINIVTKDGDPFFSGKVEYNTDHLIGDGRNTDVVKLAIGGPVLPFASQEMKEKFT
                     FYLNGAGEWLDGRLKDYYISDPNGDYTLNGRSLIDADYPIYNPYTGRDKILGIDIGNR
                     NYNSYNINLKTKYAFKPGQVATFAVRGDRSLNYPYSNNWRYALQHYAEDEIIQRQYIG
                     TYDYVINDKMNIKMKASYYQKDSYEGPKGIDRDSYIFMTVDPNNIPSDYIQRVALGDY
                     GYSTIDADNDGIYDYGFLPYDYWKFRLESVEEPRSIPGYLPPGYIYSAFIDDTTKILS
                     GRADFEWQINETHMAKTGLEIIKHNIKKNQLEDYLNIDEKRRVAYLNSIFDIRKYNID
                     NFVDADGNMLLDDEYLPEGIYAIVATKENPTSIADLVPIYKPEDYFAAAKAASGKRDG
                     YKAEPWQMAYYLQDKMEWEGMIVNAGLRFDLWYLGKKYYVAQDDGSFDLREFDKSDLM
                     QLMISPRLGVSHPITDKDVLRFAYNYQNQLPQMQYIFTSKTPEDAYASETTIRVGNPS
                     LEPQITVTYEAGLSHQLSDDYVLDLTAYYKNIYNYVNTRKERSEIEQTVYWYKYYSND
                     YGSARGIDMQIEKLLSNFNTWSLAYSLAWAQGNSSYTVIQDETTNLREFPLDWDIRHN
                     ISANFTFRIARGEEFFIPFTNFILPLDDFSANINWAYASGAPYTPQSYESGQELDTNS
                     KRMPSTQQTNLRITKGFLLSNKINIKVFAEVENLFKNKNIISVYPKTGNYYWNGSQIE
                     ESNIPGFVYPEVYYVNSLASKNPLYYNDFRGLTLGFSFNF"
     gene            18534..19295
                     /locus_tag="PLG69_02855"
     CDS             18534..19295
                     /locus_tag="PLG69_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPI42479.1"
                     /translation="MRKFIGTMLLIMMMTFVFASFAYAQEKPAAETAGGGLQNFAELV
                     FGSGLVKWFKNGGWAMWAILFLAIYGLAYIIWKFIALLNGKVNLNNFLNKIIPLIKEK
                     KFEEAKEIAKNTRGPVAAIIYAGLEKVDKGLTAVDEAIENASIIEMSYLEKGFIELSS
                     SITLAPMLGFLGTVAGMITAFDAIAAARAVDATIVATGIRIALITTEAGLIVAIPVQF
                     FNNVFMTMVDGIVLDMQRASDKFTEVLTEELGLAR"
     gene            19292..19717
                     /locus_tag="PLG69_02860"
     CDS             19292..19717
                     /locus_tag="PLG69_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPI42480.1"
                     /translation="MKIVRKRKYRATIPTASTGDIAFLLIIFFMSTTKFDVKEGIKLV
                     LPQAAESQENKTKTLTLTEKEMTRFQIFPDGQIAVNREAPRYIDSEELDVIIQQKVQI
                     NPDMIFKVITDREAKYNDMIRVIDRLKAAKIEKISLSTS"
     gene            19726..20163
                     /locus_tag="PLG69_02865"
     CDS             19726..20163
                     /locus_tag="PLG69_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPI42481.1"
                     /translation="MAKFSGKKRRDVVIPQASTSDIAFLLLLFFMVTTVFVQEKKFWV
                     ERERWPFAESIERIPRNHTSTIYVMRDETILLDDIPTRIESSEDVFVSQTLIRKKEED
                     PELIVCFRTDRETKYGVMSDIMRQLQDANALVVAFETQPILKK"
     gene            20174..20809
                     /locus_tag="PLG69_02870"
     CDS             20174..20809
                     /locus_tag="PLG69_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPI42482.1"
                     /translation="MNTKNQDWKDIANAQFSKALALSLTLLIFGIMVTPKVEVRKQVF
                     QSQQMELVDIPPEERERIEAPQTEVDVNVTFQISDVLGTEEVDMAAFQEALSQIGNIY
                     STTAPTQQQGEDDTPVNFVPYDDAPVVIGKIEPVYPEFAKRNKLQGTVILEVEVLKDG
                     SIREIRVRRGVGGGLDEAAIEAVRKVKFQPGKSSGQPVDCMVIIPVEFKIQ"
     gene            20823..>22284
                     /locus_tag="PLG69_02875"
     CDS             20823..>22284
                     /locus_tag="PLG69_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42483.1"
                     /translation="MKFYKIGICLCLLLFFTASAAYAAMAVPATGTKKLDLTKYHNVG
                     NIWLRVSNYGFFGSGDDIVPQYPSLEYPGGSGIDYLYQGALWFGAKKQRRDSFGKKLY
                     WLVYPPSETNDTIITDADPRWNPTKKAVIDTLVSVGFDGDDDLYEFLPAYNPLLVNNP
                     DQTENYSAYNNLDGIIYATTRKQKRGVDDDGDGKIDEDGAGFTFPFRSGSELPAQFSE
                     NFGSHFLAEVPIRDFTILDDPINAEIWFPLGFMDLSYNNSNYAFASPYDDDQDGRIDE
                     DGAPVSEQDFISFYYDYCPFGTSGERDYGKSSGANRHYPLNIRVRQMSYQWSFEYIKN
                     LVYVEFDITNMNEADTLYDCAMGIYMDCDVGPQTWGVEKASDDKSGYVKGTGYEFAYT
                     YDADFDNGITPGLVGARVCTPDPEKLKFHCWYWKVGDGPSDSNPIKIPKPGETSNEKY
                     WLLTGRNPKPSSNMYTALRPEQEDLTEWEQPTPNDTR"
BASE COUNT         7198 a         3784 c         4511 g         6791 t
ORIGIN      
        1 tgtgtaattt ttgtaatcta tgcaaggtaa aattattagt cccctaaaga gattcccaat
       61 ccccgggcag ttgccacaag atcggaatca aggtctatat aattatattt ttcaatggct
      121 tcagtgatgg gaacggcaac aatattggga tgacgataag ccaccataga accccatttt
      181 tcctgtaaaa ccagttcaaa tgccttcact ccaaactgag aggcaaggat tctatcaaag
      241 gcattgggag ttccaccccg ttgtaaatgt cctaaaatgg ttacccgaat atcaattgtg
      301 cagccggcat ttttaagttc ttgagctaaa cgcaaacctg ctcctcccag tttaattttc
      361 atagctcccg gttcatcaca ttctacaaaa gtcatatctc catctacagg tctggctcct
      421 tcggcaatta cgatattggc aaaatcgtgt cctctatcaa tccgttcatt gatggcttgc
      481 ataatgtttt tgatattata cggaatttcc ggaatgaggc aaacctctgc cccacctgca
      541 attgcagaat gaagcgctat ccatccggca aaacgaccca taacttctaa aattaaaata
      601 cgatgatgac tggctgcagt agtaacaagt ttatctactg catcggtagc aatatccact
      661 gcagtttgaa agccaaaagt aaaatctgtt gcagaaagat cattatctat ggttttggga
      721 acgccaataa ccggacaacc cagctcaaaa agttgctgtg aaatgcgttg agaaccgtcc
      781 cctccaacat taattaatgc ctcaatattt tgataacgga ggcggtctaa aaaatcctgg
      841 gagcgatcta cagtagtcca tgttccatcc ggatttttaa tgggccagtt gaaaggaccc
      901 cctttattgg tagttccgat aatagttccc ccctgaatat gaattcctgc aaccttttcg
      961 ggagtgagtt ctataatatg catagggtct tttaaaatac cattaaaagc atcaatgctg
     1021 cctaaaacct cccagttatg ttcaaaagaa gctcttttta cgatagctct aataactgca
     1081 ttgagaccag ggcaatcacc tccgccggta gcaataagta atcttttttt cataattcca
     1141 ccttttttag gttaataaat aaagcccccg aattttggct gattaaatat ctctcttaat
     1201 aagaacacga taaccggaaa gttctttaaa gccaaatttt tcatatagat gccaagcagc
     1261 aggattatct acattcactt ccaatttcag ttgataacca tattcctttg ccagcgaaat
     1321 acattcttcc ataagtttgg ttccgatacc ttgattttgt ttatttggaa caactgccat
     1381 atgatggata taaagtcgtc ggaaatcatt agttacccat acaacgccaa ctactttttc
     1441 attttcctct gcaataatca atattcccgt ttgttgcaga ttttcttgca gggattcaaa
     1501 ggaatccttg cgttccggtt tattgattcc tgttttttcc caaagagtag atacctcttc
     1561 ataaattttt ttgtttagct ctttgatgcg cttaatagaa agcattttaa ccgaattcct
     1621 gcattctgaa gatatcggga tgacgtgtaa aacgaaatac ggtatcccag acaacgacaa
     1681 ataatacact tggatcatta tccaccatca ttaaataata tccttgagtg tgttcaataa
     1741 atttgagttc ctgagtgcgg ttaccggtta aataaagttt tacggtgcaa tagggatttt
     1801 taaatttttc tgcatattcc gcttgggcat tatctataaa gatataggta ttcaaacttt
     1861 ccaggatatt cagaattttt agcattgccc tgtttgccgg atggatttta aattcattgt
     1921 gggcatcctg aaaagtccat tgaagaccat ttctggtaag ggtatattta tttttgggat
     1981 gagtaacttc aatttttagc aattcttctt ctttatgact aacgatatga ggagaacgcc
     2041 agttggaaag gtctgtatta taattattag taactttagc tttcaattga tagattttat
     2101 cggaaccggc ataacgaaaa taatcataag gattatccaa attgctgaag agagtatgaa
     2161 tgcttttttt accgtcactt acaattatat gcaatgcctc ttcgtctttc tgatgaaact
     2221 tttcaatcgc ttctttacct gttcccatag gagttttagc atattttgct gtaatcactt
     2281 cccggaacaa atcgcgaatt tttaagctat cggcttccca gttaacagga taaattaatt
     2341 tccagatatt attttgctta accattttta gagtatcttg cgcatcgtaa atctcaattc
     2401 ggttgataga taaagtatcc aaatcaaaaa cccgggcgag tttttcttga ggtctgttcg
     2461 ttcttaaaac gaaatataaa cctattagaa taagcaaaac aagcagtaat attaatttag
     2521 atttcttcat ttttaaattc cttggtgcgt ttacgatgta atgatattat gcctcctacg
     2581 gcaataagaa ttaaagaagg taataaagta gcaataattt ttacttccat tttgattctg
     2641 cgttcctttt ttgccggatc accccaaagg agattatttt tatgcatata ataagcaata
     2701 tccaaaatgc tttcctgtaa atggcgggaa cggatattga tcatatcctc acgccccata
     2761 agccaatcca cagcatttaa gattatctga taacgatttt catagatggg cttatcggga
     2821 tcaacataaa gttccctatc tccgaaaata acaattttac catctttcac tgcagaaaca
     2881 aaatccgacc tttgaaactg cggtttctct ttaaaatagc tttccattct gccttcaaca
     2941 attgccccta aaggaattgg aggatttaca aaaacatccg gatcgggatt gaaaaacaaa
     3001 cgggaatcca actgataatc gggtccttct aaaagagcac tgcgagtgga agtggcaaga
     3061 atggtttgaa attttaatcc cggctttcta ttaaaaataa ttccatttcc catataaatg
     3121 ataatgttgg atatattgcg agtgatagga tgttgtgaac cgcgtaaaac aggataaata
     3181 ggaaaagaga tattagtatc cacacccatt tgtctggaat cgcagaaaat atccatagct
     3241 atgtcactgc ttaattttat gccgttattt tccagaaaat caaaaagatt ggaatgtatt
     3301 tccattactg tttcgccatc agaaaagatt ttatcagcca gaactaccag ttttcctccc
     3361 tgcattaaaa actggtctaa attgtaaagc tgggtaatag ataaagaatc ataaccggaa
     3421 tgaactatca taacaggtgt ttttggtggt ggttccagca gattggtaaa atgaacatta
     3481 taattggagt aaagttcatt ttcatatttc tgcctgggca tcaaagtata gagagtatcc
     3541 acaaaagcag taatatcggg caaagtataa cgcgtaattt tctgaatttt cagggtcatt
     3601 tgatattcca gctgattttg cattttaggc aacacattca tagattcaaa attgccctga
     3661 tattcaaaaa ccaatccgta aataacttct tttgtagtgg tcttatcatt ttcaaaaatg
     3721 cggaaataca tagccccgac tccatattgt agagctttgt ttttgaggtc ttcaggactt
     3781 ggtccgttta taacttcata atgaaatttg cctttccccg cttgtttata ttcttctaaa
     3841 aggtctttaa cataacggtc taaaatcagc atagccgcgg gtaattcttt actggagtaa
     3901 agcttaacta ccatattatc ttgcaaatta cttactgcat ccttgcttac ctgagaaagt
     3961 gaatatgctt tattcttaga aaaatcccaa cgcactctgg cataagaacc gattagcaaa
     4021 accattaaaa caatagcaag cttaataagc aggttggata aaatggaact ggtctttttt
     4081 tcttttttca ttttattttt cctgcatcag gtttttgctt tggagattaa attgagccaa
     4141 caacgcaaaa ataaccgtaa cagcaaggaa aaagagaata tcgcgagtgt ctataacccc
     4201 tttcagaaaa ctgcttaaat gataatcaaa actgaagtat tgaacaattg gaaccagacc
     4261 aaaaggtaat agcggtaaaa gaaactttaa taaataaaaa acagctgaaa taagtaaagc
     4321 caaaacaaaa gccagaattt gattgctggg aaaactggag gcaaaaactc cgatggaagc
     4381 ataagctaat cctgctaaaa taagacctat atagccgcaa attatggctc cgtaatcaat
     4441 tccctctccg aaaataacaa taatcccgaa gaaaacaaga gaaagaataa taatcgtaat
     4501 aatctgaaat aaagccgata aaattttccc ccaaacaata gaagaaagct ttatcggtaa
     4561 agtggaaagc aattctaaag ttccggtttg cctttctttg gcaatgcttc ccattgtgat
     4621 agccggaata taaaacaaaa agacaatatg gataacttca aacaaaaaac gcaattccgc
     4681 tctggctatt ttaaacacca tattggaaaa aataatgcca ataatcacca aaaacaggat
     4741 gtagacaata taagtggaaa ttgaatgcaa agcaagttca tattcttttt tagcaatggt
     4801 gaaaacggtt ttcattgctc ttccctatta tcttctgttt cagtgggttt tgtttgttct
     4861 tcttccataa attcttgttc tgcagataac ttttcttcaa tttcgggctc ataatagttt
     4921 tctatagtaa gctcatggaa aatagattcc aaactcattt tgctggtgga aaattctaaa
     4981 atcaaccagc ccctatctct gataaaacgg gaaagctctt ggcgtaaatc aaattcggag
     5041 gtttcatcaa tactattctt atcgctttca ttattggtga ttgatgagga gatcaaccct
     5101 ccggaggttt cataaacaca aactttgcag gtagtttcat tccgggaaag aatatccagt
     5161 tccacaaaag gatactcttt tagaaaatca gaaaaatcta cattttctcc ctgcacttcc
     5221 agaattaaca tagtgcttct tttcagataa ttgggcaagt tttctatatt atcatcaaca
     5281 ataattttgc ctttattaat tattacaacc cgatcacaaa gtgcctgaac ttcctgcata
     5341 atatgactgg aaagcaaaac cattttctct ttgcccaatt cccgtataag ttcacgaatt
     5401 tcaattattt gattggggtc aagaccggaa gtaggttcat ctaaaatcaa tattctggga
     5461 tcatgtaaaa ttgcctgtgc caaaccggtt ctttgacgat agcccttgga aagggttcct
     5521 attctttgat acaagacatc tttaagcccg cagtttttaa ctacaaaatt caggcgttcc
     5581 cgaaaataat cccgtttcaa tttccgtaaa gcagcaaaat atgctaataa ttccactact
     5641 gtcatttcat cgtataaagg attttgctcc ggtaaatagc caatttgtct gctggctttt
     5701 aagggatcgg taaaaatgct ttcgccgtca atttcaatat ttccggaact gggttgcaaa
     5761 taaccaacaa tcattctaag agttgtagtt ttaccagctc cattaggacc taaaaaaccg
     5821 acaatttctt tatcctgaat gcagaaactg atttcatcaa cagctttgat ttctccgaaa
     5881 tatcggctca gcttatctat ttttatcatt ttacataaac attttattat tattggtaac
     5941 cggttcgttc agcaaaatta tgtctccttc cttaagacct tctatcactt ccacttgcaa
     6001 caaatcattg gtaccaagct ttacaggtgt ggcaaccgaa ttgggagctg aagatgtttt
     6061 ctccttgctt ttggtttctt tagatgaact tttagtgtct gtaaccagat aaacaatatc
     6121 ctgatttaaa tcgttgctga agacggctcg gataggaatt accaaaacat tttggcgcga
     6181 atcagccaaa atagttacat ttgctgtcat tcccggttta acaactttac cgctagcatt
     6241 aagattaatt tcaatgggaa agaccttagc attattttct ataattgctt gcggtgcaat
     6301 ttttaccacc ttgccattgt attgttcata aggaagagca tccaaagtta tttctgcgct
     6361 ctgattcagt ttgaatttgg aaatatctac ttcgttgatg ttgcttttta caatcatctt
     6421 gtttaaatcg gcaattttca taatcacagt accttcacca aaactggtta aactggattg
     6481 aaccatttca ccttcgttaa tattacgctc aataacaact ccactggcgg tagcataaac
     6541 atgagttact tttcccggaa catccagatc acgaatcatt tcatattgac tgctggcttg
     6601 agtatattca atttctgcct cttttaaggc atccgatgcc ttatcatatt cttcctgcga
     6661 aataaattgt ttttggagaa gaattgtttt atcagccaaa tctttacgcg catttttcaa
     6721 gttcatttct gctttttgca gcattgcttt agtattgaaa agagtatttg cctgattata
     6781 atctggttca atatcggcaa ttatctgtcc ggaagtaaca taatcatttt ccccggcata
     6841 aaacttaaca attttcccgc ttactttgga ttttagggaa accacaactt ccggttgaac
     6901 ttcacctgta atttctatgc gggaagaaat atctccccgt ttaactgtat aggtttctgt
     6961 ctctgtaggt tttactgttt cagtttgctt tttcttaccg cagctttttc ccaaaaacaa
     7021 aaccagcaga atcagaagca ctataactat aattatccac catttcttac gcataatacc
     7081 tcttttttat agtatctgat aatataaaac aaaggttgaa atggcagaga ttgtgtcaac
     7141 ttaaaaaagg tcttgaggtc tggaggtcaa gaggtctgga gaaaatattt tttggggtgg
     7201 acttggcgtc ttgttgtttt cagtgtaaaa taaatctgtg ctctctgttt aatttgaata
     7261 atctgggaaa gatgatatat aagacaataa gaattttggt tgacttatac cttgctataa
     7321 aaaataaggt attttacgaa tatttatggt gggacaatga acaagctttt tatgcccgaa
     7381 ctggtaaaga ttgtagagaa gttcgagaac aaaaatgcct gcctgcaata tatggcaaat
     7441 ttgctatcgg aaagcggctg tttaagtttt ccagaccgtt ttctggcagc agttaaagga
     7501 agagaagaaa taatgagcac gggtataggg aaaggaattg ctattcccca tgccagggac
     7561 ttaacagtta gctgtttaag gatagctgtc tgtttagtga aacaggattt ggatttctcc
     7621 agtgtggata atttacctgt gcatttggtt tttatgattg ccgttcccca aagttccaat
     7681 caggattata tgaagatttt acggtcatta tcggaatttt tgaggcggga agaaaacagg
     7741 gaaacattgt tacaagccaa agatgaaagg gatttgtatg aaaaagttca atttttggaa
     7801 gaagaactac ttcctcagct tggcgtttag tattcttttt ctaatgccat tgctggcaca
     7861 gcatcaagat gccggaacta ccggtttttc tacattaaag ataatttatt ctgccagagc
     7921 aaatggaatg ggacaagcaa tgctgggaag agcaaaaaat tttgatggta tgcagtttaa
     7981 tcctgcatcc ataatcagag ttcccaatca gggtgttttt actacctttg cagaccattt
     8041 tgttggttcc ggaggtggtt cggtaagtta tgtagttccc aaaaatatat acacttctta
     8101 tggattttat ttaaactatt ggaattcggg ttccatagac aggacggatg tagggacaaa
     8161 tggcgaattt attgaactga atgatacctt taatgcccaa gatatattag ctggctttac
     8221 tttggcaaaa ttcgtaagcc ctgctttgga tgcgggaggt ggtataaaat ttgttttaga
     8281 ccaaatagat gataaatccg cttctgccgt tttaattgat atcggtattt tgcatcatac
     8341 tgccaacgaa agaataaaag taggtctatc ggcgaaaaat ttgggtttcc aaattagcca
     8401 ctacagcgat aaaaaatatg atgaaggaat accatttact tatggtgcag gttttggaat
     8461 agatgtgggg aaaaatactc tgctgaacat agatattact aaagccaacg gagaaaactt
     8521 tatcggtaaa tttggagttg aacaacaagt tcatccttcc ttagttctaa gaggcggatt
     8581 taaaactaat gccggggact attatctcgg aggaaatatg gaatggactt ccggaataag
     8641 tttaggagtg ggctgggttt ggaaaaaaat tgccctggac tatgctgttg cttcttacgg
     8701 ggacttagga ttaaccaatc aggtaagtat aagatataat attgaataac tctatacaat
     8761 aataattaat gataggatag aatttggaac gaagccggtt tttactggaa ttgggatgtg
     8821 aagaactccc ggaaagacag atagaaattg cctgtaacgg tgtcagctca tcttttgcga
     8881 cttttttgaa aagtaataaa ctatattgcg gttcttataa agtgagtgga actcctcgca
     8941 ggatatattt agatgctctg gaactggata ctcatcagga ggatgaggaa attttaaaga
     9001 caggaccttt agtcagcgtt gcttacgatg aagagggcaa tctaacacct gcaggtaagg
     9061 gttttttaaa caaaagccag gcaaaggaag cagcaatatt tattcaggaa acagagaaag
     9121 gtaagtttct ggcagtaaaa tatataaaaa agggattagc gacagttgaa ctgctgaaga
     9181 actggattcc ggaaatgatt gtgcagatac catttgagaa aaaaatggct tggagccaat
     9241 taggattaat tttttcccgc cctctgcgct ggtttttaat tctatggaat cataaaccgt
     9301 tagagttgga ttttggggga ataccttgcg ggaatattac ttacggaaat cgttttttgg
     9361 ggttggataa gtccattact attaataaag ctgaggacta tcttcccaaa ctgcttgaac
     9421 agaaagtaat agctgatatt gaagagcgca gaaaagaaat aattacccaa ctggcagcta
     9481 tttttccgga aggcaatttt aaagttatag aagaccgtga gttagtagaa acggtagtta
     9541 atttggtaga atatcctact gcggttgttg gagaatttga agaaaagtat ctttccctac
     9601 cggataaaat tattatcagc acaataaatc aagcacaaaa gtattttgcg gtgcaggaca
     9661 accaaggtaa attaagtaat aaatttgtat ttatttccaa tggtaatcca tctgcaagta
     9721 tgattatcag aaaaggcaat gagaaagtag taaaagcacg cttggcagat gctttatggt
     9781 attggaatga agatacaaaa tatccgctgg aatattggac gaggcatttg gacaaggtta
     9841 tatttcaagc gaaattagga acaatggctg ataaaaccaa acgcataatg caattggtaa
     9901 gtgccttagc ggatgaattg aagataacag aagaacaaaa gaaaaaggca attcgttgcg
     9961 cagaactttg taaagcagat ttggttacta atatgcttgg agaaaaagaa tttacaaaac
    10021 ttcagggata tatcggcaaa caatatgcta ttgcttcggg agaagatctg gaagttgcag
    10081 agggtatatt tgaacattat ttaccaagag gttcgggtga tgaacttcct tcaacattgt
    10141 gtggagcaat agttgctatt ggagataaaa tggatacggt tgccggaatt attggaattg
    10201 gaatgatgcc aacgggaagt ggagatcctt ttgccctccg acgcgctgct aatggtattg
    10261 tgcaaattat cagctcccga aaatgggatt tggatttgtt ctcttttgcc gatagagcat
    10321 tggaattaat tggtaaacaa acagaattag acaaaaaggc aaaaaagaat ttgcacagtt
    10381 ttctggaaca aagaattact ggattgctga aaacaaacgg tatcgcttac gatgtgatag
    10441 aaagcgtgat gcatattgat aaaagccatt tgcacgattt ggaaaaccgg gctcaagcat
    10501 tgaatgcatt aaaaggtaaa gaggacttta ttcatttggt gattggtttt aagagggtag
    10561 caaatattat tgctgaaaca caggattttg ttccctttaa taaagataaa cttgttgagc
    10621 cggaagaaat taacctttat gaagctctgc agatattaca tcaggatatt gattccgctt
    10681 tacaaaacaa ggattatcct ttggctttac agtatttgat caagtttgga aaagttatag
    10741 acgccttttt tgacgccgtt ttggtaaact gcgaagatga tgaattacgc aataatagac
    10801 actccttgct gaaagaagta aaaaaagaat ttttaagagt tgcagaccta tcgttgatag
    10861 ttctggaaac ggaacaaaag gaagcaggaa aatgttcata atagatgagt taataaacaa
    10921 agcaaaagga aactccggaa gtattgtttt gccggaatcc actgatccac gcgttttaac
    10981 tgcggttgcc aaaattgtgg caatggactt ggcaaaagtt accctgctgg gtgaaagtga
    11041 aataataaat caagaagcca aagcacttaa tatagaatta aacaatgtga ccataataaa
    11101 tcctgagacc tcagaagaca ttcccgcttt tgctgaagca tattatcaaa gacgaaaaga
    11161 gaaaggtgtt acaatggctc aggcaataga gacagttaaa aatcctttat atttcggtgc
    11221 ttctatggtt aaagagggaa aagttgaagg aatggttgcc ggagcggcta atattacagc
    11281 agatgtttta agggctgcat tacaagttat tggtgttatg ccggggctta aaactgtttc
    11341 cagcgcattt ataataccgg ttcctgattt tcggggggaa aaaagaattt ttttatttgc
    11401 cgattgtgct gtagttccca atcccagttc agaacagctt gctgacattg ctatgagcac
    11461 tgctttaacc cgaaaagcta taatcggaga tgagccaaaa gtagctttac tttcgttttc
    11521 cactttagga agtgctaaac acgaacttgt agataaagta actatggcta aagaaatttt
    11581 aacagaaaga aaggtggatt ttgattttga tggagaatta caggttgatg cagctataat
    11641 tcccgaagta gcaaaaagga aagcacccca aagttctgtg gcaggaaatg ccaatatcct
    11701 tattttccct gatttacagg caggaaatat tggttataaa ctggctcaat acataggaaa
    11761 agtagaagca atcggaccta tcatacaagg tttagcagct ccggtaagtg atttatcccg
    11821 gggttgttct gctgaagata ttgttaatac agtcgctatt gttctgcttt tggcggacca
    11881 acaaaaaaag aaataaaaaa aagatagatt ggaggtgaag atggctatca agctatccaa
    11941 ccgctgccgt ttaattaagc cctcgccaac attaagttta tcggcgaaag caaaagaaat
    12001 gaaggatgcc ggaattgatg taatcagttt tagcgtagga gagccagatt ttaatacccc
    12061 ggaatatatt aaggcatcag cacataaagc tatagatgcc aattttacgc gttatactaa
    12121 taatgccgga ataccggaat tgagacaggc aatttgcgag aaattattac gggataacgg
    12181 gttaaagtat agccccaaag aaattctggt ttcacctgga gccaaggcag caattgtaaa
    12241 ttccctgatt gctgtgtgtg atgataaaga ccaggttttg atggcaactc cttattgggt
    12301 aagctatcct tatcaggttg ccttagctaa tgcagaaccg gtttatattc ccacctgtga
    12361 agaaaaggga tataaaattc aacctgaaga cctagaaaaa gcaataaaag agaatccctg
    12421 ctccaaagtg ctgataatga attcacccag caatccaaca ggcacagttt atacccaagc
    12481 tgaactttcc gctatcgctg agatatgtat caaatataat atattggtta tatccgatga
    12541 gatttatgaa cgcttggttt atgatgaggc aaaacatata tcaatagcaa gtatcagtga
    12601 agaaatgaaa caaaggacta ttgtaataaa cggtgtttct aaagcttatg caatgactgg
    12661 ttggcgtctt ggttatgcag caggacccgg tgatattatt tcagcaggag ctatggttca
    12721 ggaacataca acttcctgtg taaattctat tacacaatat gcctgtgtaa cagcattaaa
    12781 ggaagaagac gattccatag aaaagatgcg agtggaattt tcccgtcgcc gggatttttt
    12841 atatgaagag ctgaaaaaac ttccgcatat tacctgtttt aagccccaag gagcttttta
    12901 cattatgccg ggaataaaat ggtatctgga gaacaataac cagaatatta agacctccga
    12961 tgatttttgt gctaaattac ttgacaagta ttatgttgct ctggtatcgg gtaattcatt
    13021 cggaatggaa ggaactgtgc gtttttctta tgctaatagc atagaaaaca taaaagaagg
    13081 actaaatcgc tttgcttctt ttctggcaga gttagcttcc gggaggtaaa gatgaacgat
    13141 tttaacgata agatgaaaga acgctggaag gataaacgta aaaaagcata taattggcct
    13201 aggctaataa ttatggtgct tgccttaata atcattcttt atcttatgaa ccacctcggg
    13261 aatacaaaaa atgtagttca aaatccttct gcaagtgtta ccgacacttt acaagcagat
    13321 acattgcaga aggagatcac accttgagtt tagtaatagt tggttcaata ggtttggata
    13381 gtatttccac acctgctgga gaaatacaaa atgctttagg gggctctgct atttatggtg
    13441 ctttagcggg ttcctttttt acagatgctt atattgtagg agtagtcgga acggattttc
    13501 cgatagatgg acttaactta ctgcagaaaa aaggaataaa tcttgacgga ctggagctta
    13561 aagagggcaa aacatttcgc tggagcggaa aatatagtaa ctggaataaa gtggaaacgc
    13621 tttctacaga gttaaatgtt tttgctgatt tttatcctca attgcccgag tcctgtaaaa
    13681 gctgtcgtag tttattatta gctaacatca atccttccct gcaaatgcag gttttaaatc
    13741 aggtatccgg ttattcacat cttgcctgtg atactatgaa ttattggatt tatttgtgtc
    13801 ccaaagaaat agaacaagtg ctgaggagag taaatattgt ttttatgaat gaggacgaag
    13861 ttagagagta tacaaataag gagaatattt attctgcagc cagagagata ctctctttgg
    13921 gaccgcagtg gataattgta aaacgcggtg aatacgggtc ggtagctatt tcaaatgacg
    13981 atatgtattt tgcgcctgct tatcccttag aagaagttaa agacccaaca ggagcaggag
    14041 atagttttgc cggtgctttt atgggatatt tggcaaatca taatatctta aaccgagcag
    14101 taattaagga agcaatttta tatggaacag ttgtatcggc gttaaatgta gctgatttca
    14161 gtgtgaaagg tttaattgat aaaaccaagg cggaaattga tgtgaataag gaacaactgg
    14221 taaaatggac aacttgaaaa atggactgga ttatcgcaaa gcgggagttg atatacaagc
    14281 tggagaagta actgttttag caataaaaca gaaagttcgt agcacctaca acagcaatgt
    14341 ccttagtgaa ttaggcggat ttggcggact ttataaaata gataaaaatt tatggcatca
    14401 gccgattctt gtttccagca ccgatggagt gggaaccaaa cttttaattg ctattatggc
    14461 tgacaaatac gaaacaattg gtcaagacct ggtaaatcac tgtgtaaatg atattttagt
    14521 tcagggtgca atcccgcaat tttttctgga ttacattggt atcggaaaac tggagccggc
    14581 aaaagtggaa aaaatgattg aaggaatcat cattgcctgt cagcaaaatt cctgtgcttt
    14641 aatcggaggg gaaatggcag aaatgccgga tttgtatgaa gatgaagaat ttgatctggt
    14701 gggcacaata ataggtattg tagaacagga acaaatattg cccagaccta aaataaaacc
    14761 tggtgaccaa cttatttctt tgccaagcag tggattacat actaatggtt attccctggt
    14821 aaggaaaatt gtgtttgaga aactaaaatt atcgctggat agctatattc ctgaatgtca
    14881 aactaccttg ggtgaattac ttttaagtgt tcatagaagt tatctgcctt tactgaaaga
    14941 ttatcttacc gaccctaatc ttaagggatt agctcatatt accggaggtg gtattatcgg
    15001 aaatttaaaa cggattttac cagcaggtat tagtgctcaa attaaactca ggaaaacaga
    15061 aatacctcct ttctttcatt ggttgcaaga ggcaggaaaa ctatccgagg aaacaatgag
    15121 agaggccttt aatttgggaa cagggatgat ctgtatagtt gataatgagg catttgataa
    15181 atacttaact ataccggaag caagatgtat aggcgaactt gtgaaccaaa atccgacaaa
    15241 agaaaaagtg gaattcattc agtaatgtta aagttaagaa atttagatag ttttcacctt
    15301 gttagaagag gaattttgat tatattataa aaaaacctct tgactttttt cttaagatta
    15361 caaagaagag tttttgagta agaaattaag atagagggaa aagcttattt gagcttggaa
    15421 ctataagtaa aataaaggta taaaacccag aagataggga ggttttcgat gcgtaaatat
    15481 gcattgatcc tactaataat tatgcttgca ggaacagtat ttttagaagc tgctactact
    15541 ggaaaacttg cagttagagt gcgcgataat gccggtcgcc ccttggaatt tgttaatatt
    15601 gttgttatgc aaggaaatca acgaataaca ggtggtcaaa cgaatgctaa aggaacagca
    15661 attatcataa atattccccc aggtctttat acagttaagt tttctcttat cggttatgat
    15721 actgtggtgt ataatgatgt gcgtataatt gtagatcaaa ctacaaatct tgctcctact
    15781 atgaataaaa gcggttttga aatgaaggca gtaaccgtta cagctactga agataaagta
    15841 gataaaaacc gtgtaggaag tgccagaaat atagaaatga gtattgtaac agatgctgct
    15901 gtttcagatg ttgctgatat tgttgctttg caagcaggag taactaatgt tggaggcgaa
    15961 ttacatattc gtggtggccg ttcaaatgaa attaattata ctatagatgg tatgagtgtt
    16021 accgaccctg ttgacggagg agcgacttta caagttgata ccgatgctat taaagatatg
    16081 aaagtgatga caggaggttt tccagcagaa tatggcaatg ctcaatcggg agtgatcaat
    16141 attgttacta aagatggaga tccttttttc tccggcaagg ttgaatataa taccgatcat
    16201 cttattgggg atgggcgcaa tactgatgtt gtgaaattgg ctattggggg tcctgtatta
    16261 ccctttgctt ctcaggaaat gaaggaaaaa ttcacatttt atctcaatgg tgccggtgaa
    16321 tggttggatg gtcgtttgaa agattattat ataagtgatc ccaatggtga ttatacttta
    16381 aatggaagat cgctaattga tgctgattat cctatctata atccttatac gggtagggat
    16441 aaaattctcg gtattgatat tggcaaccgc aattacaatt cctataatat aaatttaaaa
    16501 accaagtatg cttttaagcc cggtcaggta gctacatttg ccgttcgggg cgatagaagt
    16561 ttaaactatc catatagtaa taactggcgc tatgcacttc agcactatgc agaagatgaa
    16621 ataattcagc gacaatatat tggcacttac gattatgtaa ttaatgataa aatgaatatt
    16681 aaaatgaaag ccagttatta tcagaaagat agttatgagg gtcccaaagg cattgatcgg
    16741 gattcctata tatttatgac tgtagaccct aataatattc ccagtgatta tatccaaagg
    16801 gtagctttag gggactatgg atattccaca attgatgcgg ataatgatgg tatttatgat
    16861 tatgggtttt taccctatga ttattggaaa tttcgtctgg aaagcgtgga agagccaaga
    16921 tcaattcctg gctatcttcc tcccggttat atttatagtg ccttcataga tgataccact
    16981 aaaatactaa gtggacgagc agattttgaa tggcaaataa atgaaactca tatggcaaag
    17041 accggtttag aaattataaa gcataacatc aaaaaaaatc aattagagga ctatctgaat
    17101 attgatgaaa aacgacgcgt tgcctattta aacagcatat ttgatattag aaaatataat
    17161 attgacaatt ttgttgatgc cgatggcaat atgttgttag atgatgaata tctccccgag
    17221 ggcatttatg cgattgttgc aactaaagaa aatccaacat ctattgctga tttggttcct
    17281 atttataagc cggaggatta ctttgctgca gctaaagcgg catccggaaa gcgtgatggt
    17341 tataaagcag agccctggca aatggcatat tacttgcagg ataaaatgga atgggaagga
    17401 atgattgtta atgccggatt acgatttgat ttgtggtatt tgggtaaaaa atattatgtg
    17461 gcacaggatg atggtagttt tgatttacgc gaatttgata aaagtgatct gatgcagtta
    17521 atgatttctc cacggttggg cgtttctcat ccgattacag acaaagatgt tctgcgtttt
    17581 gcttataact atcagaatca attacctcaa atgcagtata tttttacgag taaaactcct
    17641 gaagatgctt atgcttcgga aacaacaatc agggtaggaa atcccagttt ggaaccacaa
    17701 ataactgtta cttacgaagc aggtctttct caccaactta gcgatgatta tgttttagat
    17761 ttaactgcct attataaaaa tatatataat tatgtgaaca ccaggaaaga gcgtagtgaa
    17821 atagagcaga cggtatactg gtataaatac tactccaatg attatggttc tgctcgggga
    17881 attgatatgc aaattgaaaa actgctatct aattttaaca cctggtctct tgcctactct
    17941 ttagcttggg cacaaggaaa tagttcttat actgttattc aagatgaaac taccaattta
    18001 cgagagtttc ctctggattg ggatatacgc cataatataa gtgccaattt cacattccgc
    18061 attgcccgag gagaagaatt ctttatacct tttactaact ttatattgcc tctggacgat
    18121 tttagcgcta atatcaattg ggcttatgct tcaggagcac cttatactcc acaaagttac
    18181 gaatctggtc aagaacttga cacgaatagt aagagaatgc caagtactca acaaacaaat
    18241 ttaagaataa ccaagggctt tctcttgtct aataaaatca atattaaagt ttttgcggaa
    18301 gtggaaaatc tatttaaaaa caagaacatt ataagtgtct atcccaaaac agggaattat
    18361 tactggaatg gctctcagat agaagaatcc aacattcccg gtttcgttta tcctgaggtt
    18421 tattatgtaa attccttagc aagtaaaaat ccgctttatt ataatgactt ccggggactt
    18481 accctgggct tttcctttaa cttctaaatt attccaagga ggaatcaata aaaatgagaa
    18541 aattcatcgg aacaatgctg ctgattatga tgatgacctt tgtcttcgca agttttgctt
    18601 acgcacagga aaaacctgca gctgagacag cgggtggtgg tttacagaac tttgcagaac
    18661 tggtatttgg cagcggactg gtaaaatggt ttaaaaatgg cggatgggca atgtgggcca
    18721 tcctatttct ggcaatatat ggcttagcat acataatttg gaagtttatt gccttgctca
    18781 atggaaaagt aaatctgaat aattttctga ataagattat tcccttgatc aaggaaaaga
    18841 agtttgaaga agcaaaagaa attgccaaga ataccagagg tcccgtagca gcaatcattt
    18901 atgccggact ggagaaagtg gataaaggat tgactgcggt agatgaggca attgaaaatg
    18961 cttcaataat tgaaatgagc tatctggaaa agggatttat tgaactgtca tcttccataa
    19021 ctcttgctcc tatgttagga tttttaggaa cggttgccgg tatgattact gcctttgatg
    19081 ctattgcagc tgcccgagct gtggatgcaa caattgtggc aaccggtatc cgaattgctc
    19141 tgattacaac cgaagccgga ttgattgtag ctatccctgt tcaatttttt aataatgtct
    19201 ttatgacgat ggtggatggc attgttttag atatgcaaag agcttccgat aaatttacgg
    19261 aagttttaac agaagaattg ggtttggcaa gatgaaaatc gtacggaaga ggaaatacag
    19321 agcaacaata ccaactgctt ccacgggtga tattgcattt ctgttgatca tcttctttat
    19381 gtccactacc aagtttgatg tgaaagaagg tataaaattg gttttgcctc aagcagcaga
    19441 gtcacaagaa aataaaacta aaacccttac cttaacggag aaagagatga cccgttttca
    19501 aatttttcct gatggacaaa tagcagtgaa tagagaagca ccccgataca ttgatagcga
    19561 agaattggat gtaataattc aacaaaaagt gcagataaat ccggatatga tcttcaaagt
    19621 tataacggat agagaagcaa aatataatga tatgatcaga gtgattgatc gcttgaaagc
    19681 tgctaaaata gaaaaaatat ccttatctac aagttaggag caaaaatggc taaatttagt
    19741 gggaaaaaaa gacgggatgt tgtcattcct caggcatcta cttccgatat agccttcctc
    19801 ttattgctgt tctttatggt aactaccgtt tttgttcagg aaaaaaagtt ctgggtggaa
    19861 cgagaacgat ggccctttgc agaaagtatt gaacgtattc ctcgtaatca tacaagtact
    19921 atttatgtaa tgcgggatga gacaatcctg ttggatgata taccaacccg catagaaagt
    19981 tctgaggatg ttttcgtttc acaaactctt atccgtaaga aagaggagga ccctgaattg
    20041 attgtctgtt ttcggacaga tagagaaaca aaatatggag taatgtcgga tataatgcga
    20101 caactacaag atgccaatgc tcttgtagtt gcttttgaga cacaacctat actaaagaaa
    20161 taaagggaga aaaatgaata caaaaaatca ggactggaaa gacattgcta acgcccaatt
    20221 cagcaaagcg cttgcattat cccttacact acttatcttt ggtattatgg taactccgaa
    20281 agttgaagtt agaaaacagg ttttccaatc acagcaaatg gaattagttg atattccacc
    20341 ggaggagcgg gaacggattg aggcacctca aacagaagtt gatgttaatg tcacttttca
    20401 gataagtgat gttttaggga ctgaagaagt tgatatggct gctttccagg aagcgttatc
    20461 ccaaattggc aatatttata gtactactgc tccaactcaa caacaaggtg aagatgatac
    20521 acctgttaat tttgttcctt atgatgatgc acctgtagtt ataggaaaaa ttgaacctgt
    20581 ttaccctgaa tttgctaaaa ggaacaaatt gcaaggaacc gtaattctgg aagtggaagt
    20641 tctaaaagat ggcagtattc gtgaaattag agttcggcgt ggagttggtg gtggcttgga
    20701 tgaggcagct attgaagcag ttcgtaaggt aaaatttcaa ccgggaaaaa gtagtggtca
    20761 acccgtggac tgtatggtaa ttattccggt agaatttaaa attcaataat tgggagtgtt
    20821 taatgaagtt ttataagatt ggaatctgcc tttgcctatt gctgtttttt acagcaagcg
    20881 cagcttatgc agctatggca gttcctgcta caggaacaaa aaaactggat ttaactaaat
    20941 atcataatgt aggaaacatc tggttaagag ttagtaatta tggtttcttc ggttccggag
    21001 atgatattgt tcctcaatat ccttccttgg aatatcctgg aggaagtgga attgattatt
    21061 tatatcaagg ggctttgtgg ttcggagcca aaaaacagcg ccgagattct ttcggaaaaa
    21121 aattgtactg gttagtttat cctccttcgg aaactaacga cactattatt actgacgcag
    21181 atcctcgctg gaatcctact aaaaaagcag tgatagatac tttggtaagt gtgggttttg
    21241 atggtgatga tgatttatat gaatttttac ctgcttacaa tcctttgctg gtaaataatc
    21301 ctgatcaaac ggagaactat tctgcatata acaatcttga tggaattatt tatgccacta
    21361 caaggaaaca aaagcgtggt gtggatgatg atggtgacgg gaaaatagat gaagatggcg
    21421 ctggttttac ctttcccttc agaagcggtt cagaattgcc agctcaattc agtgagaatt
    21481 ttgggagtca tttcctagca gaggttccca ttagggattt tactatttta gatgatccta
    21541 taaatgccga aatttggttt cctctgggtt ttatggattt atcatataat aattcaaatt
    21601 atgcttttgc atccccttat gacgatgatc aggatggcag gatagatgaa gatggtgctc
    21661 ccgtttcgga acaggatttt atctcttttt attacgatta ctgccctttt ggaacatccg
    21721 gagagcggga ttacggtaaa tcaagtgggg caaacagaca ttatccgtta aatatacgag
    21781 tgcgccagat gagttatcaa tggagttttg aatatattaa aaatctggtt tatgtagaat
    21841 ttgatataac caatatgaac gaagccgata ctttgtatga ctgcgcaatg ggaatttata
    21901 tggattgtga tgtaggtcct caaacttggg gtgtcgaaaa ggcatctgat gataagtccg
    21961 gttatgtaaa gggaaccgga tatgaatttg cctatactta tgatgctgac tttgataacg
    22021 gtattactcc cggattggta ggagctcgtg tctgtacccc tgatccggaa aagttaaagt
    22081 tccactgctg gtattggaaa gttggcgatg gacctagtga cagtaatcca attaaaattc
    22141 ccaaacctgg tgagacctca aatgaaaaat attggttatt aaccggacgc aacccaaaac
    22201 catcttccaa tatgtatact gccctgcgcc ctgaacagga agatttaact gaatgggaac
    22261 aacctactcc aaatgatacc cgtt
//
LOCUS       DAOPFL010000021        21880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2518_length_21880_cov_94.391019, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000021 DAOPFL010000000
VERSION     DAOPFL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2518_length_21880_cov_94.391019"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLG69_02880"
     CDS             complement(<1..541)
                     /locus_tag="PLG69_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42484.1"
                     /translation="MKKLALLLLFYLLFLTALNAFNTGKSVFFADSYMLRAKGVEAAY
                     WNPANLQQEKGIDFWIPIMNLGIGINNNSLDLDTYNYVVSRDTLNDIDKQKILNKIDG
                     SLRSALEGNISIVGFAFDNMSLSSSLHLTGKLALSERYLDLLLYGNTDSLYIFDKSTT
                     DAGGLGYVDITFAMGNFTLP"
     gene            complement(543..2045)
                     /locus_tag="PLG69_02885"
     CDS             complement(543..2045)
                     /locus_tag="PLG69_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42485.1"
                     /translation="MKKIYLMLIIALILLTACEIKKPTMPTWFMDLNIPIINERYYVS
                     DLVDSVNIVVDENEVLTVTTSGEATTNEFGTLPFTPDVNVEGLPIWSTGLDLTIPFED
                     QNGKVGVAYAEIAEGIFKSRFNNLQSGVEITLTFYDLYLPNNQNLVLTSNNPGNWVTT
                     NLEGLHLGVANSDQVINQIHLSFQITPSLPSGMQVATFDFQANETIQFGEFRGTLNNY
                     PIGLEDSGSLVNIDYPYGLDQAINLEDAYLQITLQNYLGFGSVFSGQLKATNSSGQSR
                     IIDILDEYGNNYQIEPATEEGPRIVEIQFGHNVAQLLQIMPTTIQVIDGQFLISSGTN
                     IGSLRPTDTMLLFYTVSAPLTFTLHAHEITIAEEQEFSIPEENRERIRKNLVNASLNL
                     ELKNKLPIGASAKLFFSTTPSIDTNNPSTYNFMKEAAIYSANLQPDFQNVNLTLNKDE
                     LNVFTSEQVFMRFAFSFEETGTPVTIHASTMDYIHIKGMMSARVLIEKED"
     assembly_gap    2075..2174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2488..3255)
                     /gene="rnc"
                     /locus_tag="PLG69_02890"
     CDS             complement(2488..3255)
                     /gene="rnc"
                     /locus_tag="PLG69_02890"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPI42486.1"
                     /translation="MKSIISRILDYFNAKKITEQYPKWEKSLSQLQKKIDYTFHDPTL
                     LYAALTHKSYLHRHFEDHKTPSPFERMEFLGDSILGFIVSKELFTRHPDEQEGKLSKL
                     KSKIVSETYLTLKAKAIDLGKYLLLSPEEQQSGGASKPSILSDAMEALICAIYLDSGI
                     SAATRFIRNHILNNYQETVTREELVNYKSILQEYMQSRNQPPPTYVTVAEQGPEHHKT
                     FVVEVRWGNKLLGTGTGNTKKNAHQEAARMACQKLGI"
     gene            complement(3290..4531)
                     /gene="fabF"
                     /locus_tag="PLG69_02895"
     CDS             complement(3290..4531)
                     /gene="fabF"
                     /locus_tag="PLG69_02895"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPI42487.1"
                     /translation="MNKRRVVITGIGAITPVGHNVAQTWESLVNGVSGVDLISKFDYS
                     NLPVHIAAEVKNYDPEEHFDHKEVKKLDLYTQFAMIAAREAIADAGLIPGSFNPDRTG
                     VITGAGIGGILTFEEECIKNYTQGPRRISPFFIPKMIGNIAAAHISIENNFKGLNFNV
                     MSACASANHALGTAFRSIQYGDADIILSGGTEAAVTPLSVGGFSAMRALSTRNNEPQK
                     ASRPFDAERDGFVMSEGAAILVLEELEHALARGAKIYAELVGYGASEDAFHITAPTED
                     GEGSSRAMLMAIQDAGLKPKDIDYINAHGTSTPLNDKGETISIKNVFGDYAYQVKINS
                     TKSMVGHMLGAAAGIEAIVCIKSIETGIIHPTINLTHPDPECDLDYTPNQAVKHIVNI
                     ALSNSLGFGGHNSAIIIKRYA"
     gene            complement(4699..4935)
                     /locus_tag="PLG69_02900"
     CDS             complement(4699..4935)
                     /locus_tag="PLG69_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPI42488.1"
                     /translation="MMDIEAKVKQIVVDKLGVEESQVVPSANFIEDLRADSLDTVELV
                     MAFEEEFGITIPDEDQDKLRTVGQAIDYLKEKLG"
     gene            complement(4978..5727)
                     /gene="fabG"
                     /locus_tag="PLG69_02905"
     CDS             complement(4978..5727)
                     /gene="fabG"
                     /locus_tag="PLG69_02905"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPI42489.1"
                     /translation="MNWDFSDKVIVITGAGRGIGFSLATAFGEAKAKVIIIDISAETV
                     EKAVAELKTKGFQVYGYVGNVTDSVNIENIFADIVDKYGTIDCLINNAGITRDNLLIR
                     MKEEDWQMVMDVNLKGSFICTQKAFKYMMRNRNGCIINIASVIGITGNSGQANYAASK
                     GGLIAFTKSCAKEFGSRNIRVNAVAPGFIETEMTASLSPEIRAEYAKAIPLQKMGTPK
                     DVANVCLFLASDAGSYITGQTIAVDGGLTMH"
     gene            complement(5764..6696)
                     /gene="fabD"
                     /locus_tag="PLG69_02910"
     CDS             complement(5764..6696)
                     /gene="fabD"
                     /locus_tag="PLG69_02910"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPI42490.1"
                     /translation="MKTAFVFPGQGSQYVGMANDFIQKDPALKEILDNFDKAHNTNLA
                     EVMASGPEEALKQTSYTQPAILFHSISALYGFQNKSKLQADFVAGHSLGEFSALVASE
                     MITFQDALHLVHKRGEFMIKANAGVPFAMSAIIGLEPNLVKEICSEAESVGLVRAVNF
                     NTPIQTVISGTAEGVQKAGEIAKAKGAKRVIPLVVGGPFHTPLIDKAKDWLMEEMQKV
                     DFKDGKIPVISNVTALPVMNGQEERELLARQIVSPVLWVQSVQYMIDQGVQRFIEFGP
                     QKVLSGMIRNINKDVEVFSIDKYEDIDGVVSATS"
     gene            complement(6807..7799)
                     /locus_tag="PLG69_02915"
     CDS             complement(6807..7799)
                     /locus_tag="PLG69_02915"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPI42491.1"
                     /translation="MLNYYAKLSSFGSCVPQKILNNYDLEKIVETSDEWIRTRTGMFE
                     RHIASENEAASDLAYEAAVRAIESSSVKYKDIDQIIVATISGDHPFPSTACILQKKLG
                     LKDIPCFDISAGCTGFIYALDVARQYVENGIVQNILVIGVEILTKITNWKDRNTCVLF
                     GDGAGAAIVSRAEPSEISRIIDSKIAADGSQGEYLIQYAGGSRMPASHETVDKNLHTV
                     YMEGNRIFKNAIRCMYASSDELLKRNNLTTADVDWIIPHQANLRIIEGLASKMKVPMS
                     KVIVNIEKYGNTSSATIPIAADEAIRNKKIRRGDIILLTSFGAGLTWGSILARY"
     gene            complement(7802..8797)
                     /gene="plsX"
                     /locus_tag="PLG69_02920"
     CDS             complement(7802..8797)
                     /gene="plsX"
                     /locus_tag="PLG69_02920"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPI42492.1"
                     /translation="MRIAVDAFGTDNAPFPEIEGAVLAIKEDLCEEVILVGDEEKIKH
                     ELEKFFYPQKRISVVNATQRIEMEDSGATAVRSKRDSSLVKTIELHNQGLADAAVSAG
                     NTGAVMSASLLIYGRIKNVLRPAIAVVFPTETGHQILLDMGANVDCTAENLVQFAKLG
                     SMYFKYFFKEEHPRISLLNIGEESAKGNATTKQAYQLLSQEKELNFIGNIEGKDIIKG
                     ITDVVVCDGFVGNIVLKTIEGVGFSIFEILKEQINKDWIAKLGALLSYPVYTHIKNKL
                     DHTEYGGALLVGLNGISVVAHGRSNAKAMKNAIRFAALIAQSGFVTNARNYFERS"
     gene            complement(8799..8978)
                     /gene="rpmF"
                     /locus_tag="PLG69_02925"
     CDS             complement(8799..8978)
                     /gene="rpmF"
                     /locus_tag="PLG69_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPI42493.1"
                     /translation="MAVPKRRTSKSRRDKRRTHKVLTPPTISTCKKCGEPKLPHHICE
                     KCGTYNEKQILTVKE"
     gene            8922..9065
                     /locus_tag="PLG69_02930"
     CDS             8922..9065
                     /locus_tag="PLG69_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42494.1"
                     /translation="MSSTFISPAFGGSSFGDCHLISPYLLIIFLARHKQEHIIRGGNN
                     PSL"
     gene            9522..14348
                     /locus_tag="PLG69_02935"
     CDS             9522..14348
                     /locus_tag="PLG69_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPI42495.1"
                     /translation="MIIALFLDIMKKITVLLLGLFLACNFFAIARQDSPQQQPLYSAN
                     VVKIKLSQDAVNRAQLPNNAYETREKTNFNELDQLFALNGIKSITRAHIAAKDQKWVQ
                     DTGFDRWFLVHLNGIKSVEEVISNLKNKRYIEEVIPEYYAYLHNVPNDPYFANNWGHN
                     NTAQLPVYINGSHSGPGVGVIGFDSDAIAAWGETDFFGDPNIIIGILDTGVDINHPDL
                     RLVQGYDFGDNDNDPNDNSAYAGHGTCTAGIAAAIANNGIGVSGIAGGCSVMPIKVSN
                     SNGEISFTYAANALIYAADQNVDVVSMSFGAYGMEIGSDPAADSALSYAYNNGVVLFA
                     STGNYDEPAIAYPAKHPAVIAVGAASPSGERKNSNSSDGEYWWGSGYSLQEQDTAGAV
                     DILAPTILPTTDIHGSGGYSDANYYMWFSGTSCASPYAAGVAALVRSVNSGLSPAEVR
                     NIITSTATDMTINTYEGWDYWTGYGLVNAYQAVLAANPYLPQCKIVYPINNTGFVINS
                     LIPVRVEASDLDDRSVTEVRFFLDNNPDYVFSDATMPYEWIWNTAEATLGMHKIKVVA
                     IDNDGNQRQHQIVISIVFPADEGFETGDFSSLFWNNNSPQPWYVQDDIYFTGAFSARS
                     GDVENGSLSEMKLKINVTQPGDISFYRKVSARKEFSYLRFYIDDNLAVQWTGKRDWEL
                     VSCPIEPGLHTFSWTYYSYEGSYEYDNCAWIDHLILPEYENYYWPPRNLTAAGGNGFI
                     LVYWQVPEAGNPIGYKIYRNDVLLTTITGTRYTDTDVTIGVPYQYKIVAVYTDGESES
                     TSAQTATPIAEDVTEAIIGTGTTATGSAEGCPINNLRKSLHGQMIYKASELNLQGIFG
                     PIYISRLGFNIVSSPLYSLPAFQIRMAHTAAENVSQWRGEENLITYYYADNYRPVPGG
                     FEMLTLDTPFRWNGIDNILIDTAFSPANATSSYGTVCYTVTPNGYRYTRSDTNDQTDI
                     FYGGTLTVYRPNLKISTANMPNIQISANNLVFGNVLVGFETSKSFTITNTGTSLLTGE
                     ITVPNGFSIACRGKKQNTVFFSISADNSAVFTVFFTPESPGTYNGNLIITHNVDSENR
                     IIVLNAISLTAQNTPFIDGFETDSCQWTFANENQTNKWMRGSLVTQAGRGGLFISGDG
                     GISHTYITNSASVSYAYKDILIPEGTENCKLRFSWKAKGEGTGPYFDYLRVYCVEPDV
                     VPEAGIVLGTGQLSSPLNHTEIWKEADLNIPNSISGGPKRVILMWTNDATGGTQPPAA
                     VDNIRVLLNNYTDYAFVYNGLALVNVPAVSDTLGNTYYPKLTIEGITNPENYLTVTSG
                     FASLDEPFADAGIDFTLTGANFSGAEIIFYHNLGFTPYYLGYKIEGRGIWMIQSAEIN
                     WTNEFAYFTVPDFYPNATKISFCFPRQNNVLPPYLNSFTATLNESNKVVLSWISNAET
                     GISAFQIWRGNSSNLSSAIAISDLITFNPAQQNNYTYTDTTSVALTRYYYWLKVINND
                     AIENFWGPITILTSNDPPPLPPPETALLGVFPNPFNPGTKIHYALKQEGIVEFKIYNA
                     KGQLVNSFSETHSSPGTYVCEFSGKNKDGKELASGLYICSMRFKGKTYVRKMVKTK"
     gene            complement(14524..15684)
                     /locus_tag="PLG69_02940"
     CDS             complement(14524..15684)
                     /locus_tag="PLG69_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42496.1"
                     /translation="MVKREMQDHLLHLCKGFPIITITGPRQSGKTTLAKMTFPDYYYL
                     DLENLNTRRAAKEDPYSLFANKQADYIVDEFQYAPELLSTIKSISDEVQKETQFILTG
                     SNQFTLMKDISQSLAGRSAIMELLPFSLQEAYPKEKQSLNTCIFRGFYPRLLVHNIEP
                     TVFYDSYIRTYLQRDIRLLSNIQNLDDFTRFLSLCAGRTGSLLNKESLANEIGVDSKT
                     IGNWLSVLQTSYIIYLLKPWQKNISKRLIKSPKLYFYDTGLACNLLQIRNETDLANHP
                     LRGNLFETFILSEVLKFFHNRCIQPPLSFYRESNGTEIDLLIEKGNLIYPLEIKSASV
                     INNSFYRNILKLSKSNLPLGKARIVYGGNQTWENDICKHIGWQDLSSSLTLL"
     gene            15996..18692
                     /locus_tag="PLG69_02945"
     CDS             15996..18692
                     /locus_tag="PLG69_02945"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPI42497.1"
                     /translation="MLRRTKQRQKKEVTAKMEISKTFEPKDLEKKWYQFWEENGYFKP
                     REDTNQKPFTVLMPPPNVTGILHIGHVLNNTLQDVVVRYHRMKGEPTLWLPGVDHAGI
                     ATQNMVEKELAKQGITRQELGREKMVEKIWQWKNEKGNIIIDQLKLLGASCDWDRLRF
                     TMDEMLSRAVKEVFVTLYEEGLIYKGKYIINWCPRCVTALANDEVEHTDENGHLWYIH
                     YPFAEGDGYVTVATTRPETMLGDVAVAVNPKDERYSSLIGKELILPLTDRKIPLITDE
                     YVDRDFGTGCVKVTPAHDPNDFEIGIRHNLPQILVMDETGIMNANAGKEFQGLDRYAC
                     REKVLKMLTEQGLLEKTEIHSYSVGHCYRCDTVIEPYLSDQWFVKMAPLAEKAIEVVE
                     KGEVKFQPERWTKVYMHWMNNIRDWCISRQIWWGHRIPVYYCDHCSEMIVAKEMPEKC
                     PKCGGTKFTQDSDVLDTWFSSWLWPFSTMGWPDETADLKRYLPTNVLITAPEIIYLWV
                     ARMIMSTLHFVDKIPFDTVLLHGTVRDEIGRKMSKSLGNSPDPIDLINTVGADALRFS
                     MIFGTPKGADVIFSESILETGRNFANKMWNAYRFIMMNAENIEGLPAENELQLELADK
                     WIISRLQEVIIAVRAHYENLRFNDAATEIFKFMWDEFCSWYIELSKDRLNPSSALSGR
                     LTAKYILLYVMQTGMRLLHPIMPFITEEIWQGIKKIFPMEEEALIIATFPETDETLKD
                     NDIDNNMRFVQESITAIRNLRKQINLAPNQEIDIVIRFAENSQQELFENYMAYFNRLA
                     KVKTLSGGVNIAKPKAAIASVVRNIEIFLPLSGLVDIESEKNRLGKQIEKLTKELSGI
                     KAKLANPNFLNNAKEEVVTKEKEKFIEVNTKLELLQNLLSEL"
     assembly_gap    18700..18799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18867..21368)
                     /locus_tag="PLG69_02950"
     CDS             complement(18867..21368)
                     /locus_tag="PLG69_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPI42498.1"
                     /translation="MAGKNVNQKIRQNISKTSKTMPSAKTTAREKEPSAFAKHLPWVL
                     VIILFLLLSLIYFPVAYQGKAPQASDIIQWQGAAKAIIDYNATHKDNALWTPYMFSGM
                     PSYMIHFPNRYPFLENITKLTDKIINWRIFLLFIGGVGIFLLLRQLKMDPWIAFFSAI
                     AFTFSCHWVGLLEIGHNTKFRAIMYIPWVVWALFRLKEKPNILNLGLLATFLITQLRE
                     NHPQITYYLYLFIGMFWIYGLIEAIKTKQLKKFGNWTGLLIIAFAVTALAVMNPYLST
                     WEYSHYTMRGGSTGLEKSYAQQWSFPPLEIISFIIPDFFGGINQNYWGAMPFTQIYNY
                     FGIVVLALGVIALFGKHKKFSLFLWIASAIFLVLSFGSFTPVISGFFFKYLPMFNKFR
                     VPSMTLIMVQFIAVILAGLGLDTIASLENNSKWQKKLFTAFWISGVVFILFLILGQSI
                     FKGLPYTNAAEIEQYQRYNALAKLEELKSLRPGMLYKSGIMSLLLLTVSLGICYLASA
                     KKLKKVALVLLITLITFIDLYIYTGKHLKDLYPAEEREARFVAQDFDEFLLEDKDNYR
                     IYPYNMGQIRTAGEWAYYHQTIDGYSGAKLKRYDDVWKIIQGDDKNYSEFYRYLNGVY
                     EKGGVEIPTPLLDMLSTKYIIYPDSLPYAFLLNKIKPVFTSFSGANIYQNLTAYPRAW
                     FVDSLSVIPETEARLQKMREPSFNPRSLAIVESKIDGVFKPDSCFAKETYFDLHNVKY
                     DVATDKNAFLVVSEVYYPAGWKAFIDGKKTEIYPVNHILRGVIIPPGKHTLEMKFISE
                     TYKLSLTLSLVGILATVLLLVAGIGWEEKKKKLKK"
     gene            21466..21867
                     /locus_tag="PLG69_02955"
     CDS             21466..21867
                     /locus_tag="PLG69_02955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPI42499.1"
                     /translation="MIFAFTKRIYGYECDIYGHLNNANYLQLLEGARSEALYEMNLAQ
                     SDLQKKGVQLFVHYVEMSFIKAIDHDELITIKSCFTEMNRIKGKWHQEIYNSKGEKCF
                     NADITIVFASEGKAKRLPVEVYELFMHYLEK"
BASE COUNT         6686 a         4162 c         4097 g         6735 t
ORIGIN      
        1 agggaagagt aaaattcccc attgcaaagg tgatatcaac ataaccaaga cctccggcat
       61 ccgtagtgct tttgtcaaat atatacaaag aatcggtatt gccataaagc agaaggtcta
      121 aatacctttc cgaaagagcc agtttacctg ttaaatgaag tgaactggaa agagacatat
      181 tatcaaaggc aaaacctaca atgctgatat tgccttctaa agctgaacgc agagaaccat
      241 caatcttatt taggattttc tgtttatcta tgtcgttcaa agtatccctg ctaactacat
      301 agttataggt atccaagtcc aaagagttat tatttatgcc tattcctaag ttcataatcg
      361 gtatccagaa atcaatacct ttttcctgtt gtaaattggc aggattccaa taagcagctt
      421 caactccttt agcacgcagc atataactat cggcaaaaaa gacggattta cctgtattaa
      481 aggcattcaa tgcagtcaga aacagaaggt agaagagtag taacaaagct aactttttca
      541 ttttagtcct ccttttcaat caacacgcgg gcactcatca ttcctttgat atgaatgtaa
      601 tccattgtgc tggcgtgaat agtaacagga gttcctgtct cttcaaagct gaaagcgaaa
      661 cgcataaaaa cctgctcgga agtaaaaaca ttcagttcat ctttatttaa ggtcaaattc
      721 acattttgga aatccggttg tagattagca ctataaattg ccgcttcttt cataaagttg
      781 taagtgcttg ggttattagt atctattgag ggtgtagtgc tgaagaataa tttggcagaa
      841 gctcctatag ggagtttatt tttgagttct aaatttaagg aagcatttac taaattcttt
      901 ctaattcgct cccggttttc ttctggaatg ctgaattcct gctcttctgc tatagtaatt
      961 tcgtgcgcgt gtaaagtaaa agttaagggt gcacttacag tgtaaaataa aagcatagta
     1021 tccgtaggtc ttaaactgcc tatatttgtt ccactgctaa ttaaaaactg tccatcaata
     1081 acttgaattg tagtaggcat aatctgtaaa agctgtgcca cattatgccc gaactggatt
     1141 tcaacaattc tgggaccttc ttccgtagca ggttctattt gataattatt accatattca
     1201 tctaagatat caataatcct actttgccct gaggaatttg ttgcctttag ctgtccggaa
     1261 aaaacagaac cgaaacctaa atagttctgc aaagtaattt gcaaataggc atcttccagg
     1321 ttaattgcct gatcaagtcc ataaggataa tcaatgttaa ccaaagaacc tgaatcttcc
     1381 agtccaatag gataattatt tagagttcct ctgaattctc cgaactgaat tgtttcgttg
     1441 gcttgaaaat caaaggtggc tacttgcatt ccggaaggca gagaaggtgt aatctgaaag
     1501 gaaagatgta tttgattaat aacttgatct gaatttgcca ctcctaaatg caaaccttcc
     1561 aaatttgtgg ttacccagtt tcctgggtta ttggaagtaa gaacaagatt ttggttatta
     1621 ggcaaataaa ggtcataaaa agttaaggtg atttcaactc cggattgcaa attattgaaa
     1681 cgacttttaa aaataccttc cgctatttca gcataagcaa caccaacttt accgttttgg
     1741 tcttcaaaag gaattgtaag atcaagccca gtactccaaa tgggaagtcc ctcaacatta
     1801 acatccggtg taaaaggtaa ggtgccaaat tcgtttgtag ttgcttctcc actggtagtt
     1861 actgtcaaga cctcattttc atccacaacg atatttactg aatccaccag atcggacaca
     1921 tagtatcttt cgttgatgat agggatattc aaatccataa accaagtggg catcgtaggt
     1981 ttttttattt cgcaggcggt taaaagaatc aaggcaatta taagcataag atagattttt
     2041 ttcataaaca cctccttcct ttgtgtttag gactnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnaaacac ctccttcctt tgtgtttagg actggtcttc tgcttttgct
     2221 aaattaagaa caggcttata gattatgtta tctataccaa aattgacact ctatatattg
     2281 tataaaaaaa gaaatacact tttttcttct tccacttaaa aaagcttttt agataaaata
     2341 agcatttgtt cccttttggc aaattttata ctcgtatcct tgttatattc attcttcatt
     2401 tttccttaaa gtcgtcataa gtcctcactg ccaaacaaat ttaaaggttg tgtaaaaatt
     2461 aaataggtgg taacgactcc ttacttacta aatacctaat ttttggcaag ccatccttgc
     2521 tgcttcctga tgtgcatttt tcttggtatt gcctgttccc gttcctaaaa gtttgtttcc
     2581 ccaacggact tccactacaa atgtcttatg atgttcagga ccttgttctg caacagtaac
     2641 ataagtaggt ggcggttgat ttctgctttg catatattcc tgtaagatac ttttatagtt
     2701 cacaagttct tccctggtaa ccgtttcctg ataattattc agaatatgat tacggataaa
     2761 tctggtggca gcagaaattc cactatccag atagatagca caaataaggg cttccatagc
     2821 atccgaaaga atagagggtt tggatgcacc acccgattgc tgttcttcag ggcttaggag
     2881 taaatatttt cccaggtcta tagcttttgc ttttaaggta agataggtct cggagacaat
     2941 tttggatttc agcttggaaa gttttccttc ctgttcatca ggatgacggg taaaaagttc
     3001 tttactaacg ataaaaccaa gaatggaatc acctaaaaat tccatccgct caaaaggaga
     3061 aggagtttta tggtcttcaa aatgacgatg cagatatgac ttatgagtaa gagcggcata
     3121 taataaagtg ggatcatgaa aggtataatc aatctttttt tgtaattggg aaagcgattt
     3181 ttcccattta ggatactgct ccgttatctt tttggcattg aaataatcca gaatacggga
     3241 gattattgat ttcatccgtt tctcaagcgt tataaaactc catcttttat caagcgtagc
     3301 gtttgatgat tattgcggaa ttatggcctc caaaacccaa agaattggaa agtgctatat
     3361 tcacaatatg tttaacagct tgattgggag tataatccaa atcgcattcc ggatcgggat
     3421 gagttaaatt tatggtagga tgaatgatac ctgtttcaat gctcttaatg caaactatag
     3481 cttcaattcc ggcagcagca cctaacatat gacctaccat acttttagtg gaattaatct
     3541 tcacttggta agcataatca ccgaagacat ttttaatgga aatggtttca cccttatcgt
     3601 ttaagggagt agaagttccg tgtgcattga tgtaatcaat atcttttggt ttaagtccgg
     3661 catcctgaat tgccatcagc attgctctgg aactgccttc tccatcttcc gtgggagcag
     3721 taatatgaaa agcatcttca gaagcaccgt aaccaactaa ttcggcataa attttagcac
     3781 ctctggctaa agcatgttcc agttcttcca aaacaagaat ggcagcacct tcactcatta
     3841 caaaaccatc gcgttcagca tcaaaaggac gcgatgcctt ttggggttcg ttattacgcg
     3901 tagaaagagc tcgcatagca gaaaaaccgc ctaccgacaa aggagttaca gctgcttcag
     3961 ttccaccgct aagaataata tctgcgtcgc catattgaat agaccgaaaa gcagttccta
     4021 aagcatgatt ggcactggca caagcactca taacattaaa attgagacct ttaaaattat
     4081 tttctatgct gatatgggca gctgcaatgt tgccaatcat tttaggaata aagaaaggac
     4141 tgatacgacg cggaccttgg gtgtaatttt taatgcattc ttcttcaaag gtaagaatac
     4201 ctccaattcc tgcaccggta ataactccgg ttctatcggg attaaaagaa ccaggtatta
     4261 aaccagcatc agcaattgct tccctggcag caatcatcgc aaactgagtg tataaatcaa
     4321 gctttttaac ctctttatgg tcaaagtgct cttcgggatc gtaatttttt acttcggcag
     4381 caatgtgaac cggtaaatta ctgtaatcaa atttggaaat taaatccact cctgagacgc
     4441 cgttaactaa tgactcccag gtttgagcaa cattatgtcc tacaggagtg atggctccta
     4501 taccggtgat aacaactctt cgtttattca taaagacctc attatttggt tttacttacc
     4561 cctttactct gaattttatc tctatttcct ttcaactggc tttgtagtga ggaacaagat
     4621 aaacttccgt aaagggtgga tacaatcctg atacttatgc agttatcagg attgttccac
     4681 attagaggaa agggtatatt atcccagttt ttccttcaag tagtcaattg cttgaccaac
     4741 agtgcgaagt ttgtcctgat cttcatccgg aatagtaatt ccgaattctt cttcaaatgc
     4801 cataaccagt tcaactgtat ctaaagaatc ggcacgcagg tcttcaatga aattagcgga
     4861 aggaacaacc tgggattctt caactcccag tttatccact actatctgtt ttactttggc
     4921 ttcaatatcc attattcctc ctatgttatt gaagtgtttc tttatctttg ttttcgttca
     4981 atgcatagtt aagccaccat caactgcgat ggtttggcct gttatatagc ttcctgcatc
     5041 ggaagcaaga aagagacaaa cattggctac atctttggga gtgcccattt tttgtaaagg
     5101 aatggctttg gcatattcgg ctctgatctc aggacttaag gatgctgtca tctctgtttc
     5161 aataaaacct ggagctacag catttacgcg tatattgcga ctaccgaatt ccttggcaca
     5221 gcttttagtg aaggcaatca gtccaccctt ggaagcagca taatttgcct gaccactatt
     5281 tcctgtaatg cctattacac tggcgatatt gataatacag ccattacgat tacgcatcat
     5341 atatttaaat gccttttgag tgcaaataaa gctccctttc aaattcacat ccatcaccat
     5401 ttgccagtcc tcttccttca tgcggatgag caaattatcg cgcgtaatac ctgcattgtt
     5461 gataaggcaa tcaatagttc catatttatc aacaatatcg gcgaagatat tttcaatgtt
     5521 tacgctatca gttacatttc ctacatagcc ataaacctga aacccttttg tctttaattc
     5581 tgcaaccgct ttctctacgg tttcagcaga aatatcaata attattactt tagctttggc
     5641 ttctccaaaa gcagtggcta gggaaaaacc aatacctctc cctgcaccag taattactat
     5701 aactttatca gaaaaatccc agttcattat ttccccatta gtataatttt tatttttagc
     5761 aacttaactt gtagctgaca ccacaccatc aatgtcttca tatttgtcaa ttgaaaaaac
     5821 ttccacatcc ttattgatgt ttctaatcat accgcttaag accttttgcg gaccgaattc
     5881 tataaagcgt tgaactcctt ggtcaatcat atactgcaca ctttgaaccc ataaaacagg
     5941 tgaaacaatt tgtcgggcaa ggagttctct ttcttcctgt ccattcataa ctggtagagc
     6001 agtcacattg gaaattacgg gtatttttcc atctttaaaa tccactttct gcatttcttc
     6061 cataagccaa tccttggctt tgtctattaa aggagtatga aaaggaccgc caactaccaa
     6121 aggaataact cttttagctc ctttggcttt ggcaatttca cccgcttttt gaactccttc
     6181 cgcggttccg gaaatcacgg tttggatagg agtgttaaaa tttacagctc ttactaaacc
     6241 gacactttct gcttcagaac agatttcttt taccaaattt ggctctaaac cgataatagc
     6301 actcattgca aaagggacac cggcattggc tttaatcata aattcacctc ttttatgcac
     6361 caagtgtaag gcatcctgaa aggtaatcat ttctgaagcg acaagggcac tgaattctcc
     6421 taaagagtgg cctgccacaa aatctgcctg cagtttactt ttattttgaa acccgtaaag
     6481 agcggaaata ctgtgaaata aaatagcggg ctgagtataa cttgtctgtt ttagtgcctc
     6541 ttctggtcct gaagccatta cttcagctaa atttgtgtta tgagctttat caaagttatc
     6601 cagaatttcc ttcaaagcag ggtctttttg aataaagtca tttgccattc ctacatactg
     6661 agaaccctga ccgggaaaaa caaaagctgt tttcattatt tacctctttt gtgattggag
     6721 cccgctttag cttcgcatct tttttttgcg gaggaacagc gttctctgat tacacattgg
     6781 ttgacaaaga tgtcaacctt ccgttattaa tatcttgcta aaatacttcc ccaggttaat
     6841 cctgctccaa aggaagtaag caaaataata tcaccgcgtc tgattttttt gtttcggatt
     6901 gcctcatccg ctgcgatagg aatcgtagca gaggaggtat ttccatattt ttcaatattc
     6961 acgataacct tactcatagg cactttcatt ttggaagcca gtccttcaat aatgcgtaaa
     7021 tttgcctgat ggggaatgat ccaatccaca tctgcagtag taagattgtt gcgtttcaag
     7081 agttcatccg aagaagcata catacatctg atggcatttt tgaaaatccg attaccttcc
     7141 atataaacag tatgcaggtt tttatccact gtttcgtggc tagctggcat acgcgaaccg
     7201 cctgcatatt gaattaaata ctcaccttga ctgccatctg cagctatttt actgtctata
     7261 atacgcgaaa tttcgctggg ttcagctcta cttacaattg ctgctccagc tccatcacca
     7321 aaaagaacgc aggtattccg gtctttccag ttggtaattt tggtaagaat ttctacacct
     7381 ataaccagaa tattttgcac aattccattt tctacatatt gccgagctac atccaaagca
     7441 taaataaaac ccgtgcaacc ggcagaaata tcaaagcaag gaatatcttt cagccccagt
     7501 ttcttttgca aaatgcaggc agttgaaggg aagggatggt ctcctgaaat ggtagctaca
     7561 attatttgat caatatcttt atatttaacg gaagaacttt ctattgctct aacagctgct
     7621 tcataagcta aatcactggc tgcttcattt tcggaagcaa tatgtctttc aaacattcct
     7681 gtgcgggttc taatccattc atcgctggtt tccacaatct tttctaaatc atagttattc
     7741 aatatcttct ggggaacaca gctaccaaag gaggataatt tggcataata attcagcata
     7801 attaagacct ctcaaaatag tttctggcat ttgttacgaa gccggattgg gcaattaagg
     7861 cagcaaaacg gatagcattt ttcattgctt tggcattact gcgtccatga gcaactacag
     7921 aaattccgtt taagccaaca agtaaagcac caccgtattc tgtatggtcc agtttatttt
     7981 ttatatgggt ataaacagga taggaaagaa gggcaccaag tttggcaatc cagtccttat
     8041 ttatctgttc tttcaatatc tcaaaaatag aaaagcccac accttcaatg gtttttaata
     8101 caatattacc gacaaatcca tcgcagacaa ctacatcggt aattccttta ataatgtcct
     8161 tgccctctat gttaccgata aaattaagtt ctttttcctg gctgagtaat tgatatgcct
     8221 gcttggttgt agcatttcct ttggcacttt cttctccgat attcaagagt gaaattcggg
     8281 gatgctcttc cttaaaaaaa tacttaaaat acatacttcc cagtttggca aactgcacta
     8341 aattttctgc cgtgcaatcc acattggcac ccatatccaa aagaatttga tgtcccgttt
     8401 cggtagggaa aacaacggca atagcaggac gcaacacatt ttttatgcga ccatagatta
     8461 aaagcgaagc agacattact gctcctgtat ttcctgcgct aacagcagca tctgcaagac
     8521 cttgattatg aagctcaatt gttttcacca aagaagaatc tcttttagaa cgcacagctg
     8581 ttgcaccact atcttccatc tctattctct gtgtggcatt aacaacgctg attctttttt
     8641 gcggataaaa gaatttttcc agttcgtgtt tgattttttc ttcatctcca accaggatca
     8701 cttcttcgca aaggtcttct ttgatagcca gaacagcgcc ttcaatttcc ggaaacggtg
     8761 cattatcagt tccgaaagca tctacggcaa tacgcacctt actccttaac tgttaatatt
     8821 tgcttttcat tataggtgcc gcatttttca caaatatgat gcggtagttt gggttctcca
     8881 catttcttgc aagtgcttat agttggagga gttaaaacct tatgagttct acgtttatct
     8941 ctcctgcttt tggaggttcg tcttttgggg actgccattt aatttctcct tatcttttga
     9001 taattttttt agcaagacac aagcaggaac atattatcag aggtggaaat aatccttctt
     9061 tataaacaat cgcttgtgct ttctaagcat ttatgaactg aacttaaaag ggacattttt
     9121 tgtcaatcaa aatctctgtt ttgagagtag acgaaggacg ctgttcttca acaagttcca
     9181 aaaaattaga aagatggcaa gctagaatta caattttgtt gaagatagcc atcttagcaa
     9241 aaaatcttac ttcttatctt ttttaaccta taatgacaaa gtaacaacca agtaacaact
     9301 ccgtaacgag acaacggaag tgttacggaa gtgttacgga gtcgttactt aatcaactat
     9361 ataacaagca attacgcatt tcaagtcaac agggacagag aatagtgtta accttctata
     9421 ttatcaataa gatatgtaat tgtaattcgc ttttaccgaa aagaaacatt gcaccccgat
     9481 gctcaagaag aatatatttt atttaaaact tcttggctgg aatgattatt gctttatttt
     9541 tagacattat gaaaaaaatt acagttcttc ttcttggtct atttttggcg tgtaattttt
     9601 ttgccatagc caggcaagat agtccccaac agcaaccgct ttacagcgct aatgttgtga
     9661 aaataaagct tagccaggat gcggttaatc gtgcccaatt gccaaataat gcttatgaaa
     9721 caagggaaaa gaccaatttt aacgaacttg atcaattatt tgccttaaac ggaataaagt
     9781 ccatcacccg tgctcatatt gctgccaaag accaaaaatg ggttcaagat accggttttg
     9841 atcgctggtt tttagttcac ttaaatggaa ttaaaagcgt ggaagaagta atttccaatc
     9901 taaaaaataa gcgctatatt gaagaggtaa ttccggaata ttacgcttat ttgcataatg
     9961 ttcccaatga tccttatttt gctaataatt ggggtcataa taatactgct cagcttcctg
    10021 tttatataaa cggttctcat agcggacctg gggttggagt tatcggtttt gacagcgatg
    10081 ctattgcggc gtggggagaa acagatttct ttggagaccc gaatattatt atagggattt
    10141 tggatacagg tgtagatatt aatcatcctg atttgcgttt ggttcagggc tatgattttg
    10201 gggataacga caatgatccg aatgataaca gtgcttatgc agggcatgga acttgcactg
    10261 caggaattgc tgctgccata gctaataatg gaattggggt ttctggaatt gcaggtggct
    10321 gtagtgttat gcctataaaa gtatctaata gtaatggtga aatatcattt acttatgctg
    10381 ctaatgcctt aatttatgct gcagaccaaa atgtggatgt agtcagtatg agttttgggg
    10441 cttatggtat ggaaataggt tccgatcctg ccgctgatag tgctttgagt tatgcttata
    10501 ataatggagt agttctcttt gccagtacag gaaattatga tgagccggct atagcttatc
    10561 cggcaaaaca tcctgctgta atagcagttg gagctgccag tccttccgga gaaagaaaaa
    10621 actccaattc ttccgatggt gaatattggt ggggttccgg ttactctttg caggaacagg
    10681 atacagcagg agcggttgat attttggctc ctactatttt accgacaacc gatattcacg
    10741 gttctggcgg ttattctgat gctaattatt atatgtggtt ttccggaact tcctgtgcca
    10801 gtccttatgc agcaggagtt gcggctttag ttcgttccgt aaattctggt ctttcccctg
    10861 ctgaagtaag aaatataatt acttccacag ccacagatat gactattaat acttatgaag
    10921 gatgggatta ttggacaggt tatggattgg taaatgccta tcaggcggtt ttagctgcca
    10981 atccttattt accgcaatgt aaaattgtct atcccattaa taataccgga tttgtaatca
    11041 attcgctcat tcctgttcgg gttgaggcat ctgatttgga cgatagaagt gtaacggaag
    11101 tccgtttctt tctggataac aatcctgatt atgttttttc cgatgctaca atgccttatg
    11161 aatggatttg gaataccgcc gaggcaactt tggggatgca caaaataaag gtagtagcaa
    11221 ttgataatga cgggaatcaa agacagcatc aaatagttat atctattgtc tttccagccg
    11281 atgaaggttt tgaaacaggt gacttttctt cattgttttg gaacaacaac agtcctcaac
    11341 cctggtatgt gcaggatgat atttatttta cgggtgcctt ttctgcccgc agtggagatg
    11401 tggaaaacgg ttcattatcc gaaatgaagc taaaaataaa tgttactcag ccgggagata
    11461 tcagttttta taggaaggta tctgcccgaa aagaatttag ttacctgcgt ttttacattg
    11521 atgataatct tgctgttcaa tggacaggaa aaagagattg ggaactggtt tcctgtccga
    11581 tagagcccgg tttgcatact ttttcttgga cttattacag ttacgaaggt agttatgaat
    11641 atgataactg tgcttggata gaccatttga ttttgcctga atatgaaaac tactattggc
    11701 ctccgcgaaa tttgacggct gccgggggaa atggctttat tcttgtttac tggcaagttc
    11761 cggaagcagg aaatcctatc ggctataaaa tttaccgtaa cgatgtttta ctaactacaa
    11821 ttacgggaac aagatatacc gatacggatg taacaatcgg agttccttat caatataaaa
    11881 ttgttgctgt ttatactgat ggtgaatcgg aatcaacttc cgcccaaact gcaactccta
    11941 tagcggaaga tgttacggaa gcaattattg gaacaggaac cactgctacc ggttctgcag
    12001 agggctgtcc tattaataat cttcggaaaa gtttgcacgg tcagatgatt tataaagcca
    12061 gcgaattaaa tctgcaaggt atttttggtc ccatatatat ttcccgtttg ggttttaata
    12121 tcgtttcttc ccctttatat tcacttcctg ctttccaaat aagaatggct catacagccg
    12181 cagaaaatgt ttctcaatgg cgaggtgaag aaaacttaat tacttattat tatgcggata
    12241 attacagacc tgtgccaggt ggttttgaaa tgcttacttt agatactcct tttcgctgga
    12301 acggaataga taatattttg atagatactg cttttagccc ggcaaatgca acttcctctt
    12361 atggaacagt ttgctacaca gtaaccccta atggttatcg ttacacccgc agtgatacta
    12421 acgatcaaac cgatattttt tatggcggaa ctttaactgt ttacagacct aacctgaaaa
    12481 tatcaacggc taatatgcca aatattcaaa tcagtgctaa caacctggtt tttggcaatg
    12541 ttcttgttgg ctttgaaaca agcaaatcat ttaccataac taacaccgga acctccctgc
    12601 taacgggaga aattactgtg cctaatggct ttagtattgc ttgtagagga aagaaacaaa
    12661 ataccgtttt cttctctatt tctgcggata attctgctgt ttttaccgtc tttttcactc
    12721 ctgaatcgcc agggacttat aatggcaatt taattattac tcataatgtt gatagcgaaa
    12781 ataggattat agttctgaat gcaatttcgc taactgccca aaatactccc tttattgacg
    12841 gttttgaaac agatagctgt cagtggactt ttgccaatga aaatcagacc aataaatgga
    12901 tgcgaggttc tctcgttact caagccggaa gaggaggact ttttatcagc ggtgatggcg
    12961 gaatcagcca tacttatata accaatagtg cttctgtcag ttatgcttac aaagatattt
    13021 taattcctga gggaaccgag aattgcaaat tacgcttttc ctggaaagcc aaaggagaag
    13081 gaacaggacc ctattttgac tacctaaggg tttattgtgt tgaacccgat gtagttccag
    13141 aagcgggaat tgttttaggc acaggacaat tgagctctcc tttgaatcat acggaaattt
    13201 ggaaagaagc agaccttaat attccgaatt ctatatcagg cggacctaaa agagttattt
    13261 tgatgtggac aaacgatgcc actggcggaa ctcaacctcc tgctgcagtt gataatattc
    13321 gtgttcttct caataactat acggactatg cctttgttta caatgggttg gctttagtta
    13381 atgtcccggc tgtatccgat actttaggca atacatatta tcccaaactt acaattgaag
    13441 gaattactaa tccggaaaat tatttaactg ttacttccgg ttttgcctcg ttagatgagc
    13501 cctttgcgga tgcgggaata gattttactc ttacaggagc gaatttcagc ggcgcagaaa
    13561 ttatttttta tcataattta ggatttacac cctattatct gggctataaa attgaaggca
    13621 gaggaatatg gatgattcaa tcggcagaaa taaactggac aaatgaattt gcttatttta
    13681 ccgtgcctga tttttatccc aatgctacca aaatatcatt ctgttttccc cgtcagaata
    13741 atgttttgcc accctatcta aatagtttta cagcaaccct gaacgagagc aataaagttg
    13801 ttctatcctg gataagcaat gcagaaacag gaatatccgc tttccaaatt tggcgcggaa
    13861 atagcagtaa tctttcttct gcaatagcta tcagcgattt aataaccttt aatcctgccc
    13921 aacaaaataa ttatacttat acggatacaa cttctgtagc tcttacccgt tattattact
    13981 ggctgaaagt tatcaataat gatgctatag agaatttttg gggtcccatt acaattttaa
    14041 cttccaatga tccacccccg cttcctcctc cggaaacagc tctattagga gtttttccca
    14101 atccttttaa tcctggcaca aaaatccact atgctcttaa acaggaaggg attgtggaat
    14161 ttaaaatcta taatgctaaa ggacaattag taaacagctt ttccgaaact cattcttctc
    14221 caggaaccta tgtttgcgag ttttccggta aaaacaaaga tggcaaggaa ttagcaagcg
    14281 gactctatat ctgctctatg cgatttaagg ggaaaaccta tgtccgcaaa atggtaaaaa
    14341 ccaaataaac atcaacagag ctaaaaaaaa gttccgttga aactttttca tcttggtcca
    14401 atggttatta tgggataaaa tataattttt atatctaact aaatacgctt tagcaaacta
    14461 caaaggggag gagcttggaa ttatataaac attgctttaa tctgccaatt attcaacggt
    14521 cttttataat agtgtgagtg aggaggataa atcctgccaa ccaatgtgct tgcagatatc
    14581 attttcccag gtttgatttc ctccataaac aattctggct ttccctaagg gcagattaga
    14641 tttgcttaat ttcaggatat tgcgatagaa ggaattgtta attacagagg cagattttat
    14701 ttccagaggg tagattagat ttcctttttc tattaaaaga tcaatttccg ttccatttga
    14761 ttctctgtaa aagcttaaag gaggttggat acagcggttg tggaagaatt tcagcacttc
    14821 ggaaagaata aaggtctcaa aaagattccc gcgtaaagga tgatttgcca aatctgtttc
    14881 attgcgtatt tgtaacaggt tgcaggcaag tcccgtgtcg taaaaataca gcttggggct
    14941 tttaatcagg cgtttagata tgtttttttg ccagggctta agtaaataga ttatatagct
    15001 tgtttgcaac acggaaagcc aattaccaat tgttttacta tctaccccta tctcatttgc
    15061 cagggattcc ttatttaata gagagccagt tcttcctgca catagagaaa gaaaacgggt
    15121 aaagtcatcc aaattttgga tattgcttag taggcggata tctctttgca ggtaagttct
    15181 aatataggaa tcataaaaga cagtaggttc aatattatga acaagtaaac gcggataaaa
    15241 tccccgaaaa atacaagtat taagagattg tttttccttt ggatatgctt cttgcaaact
    15301 gaaaggcaaa agttccatta tggcagacct gccggctaag gactgtgaaa tatccttcat
    15361 taaagtaaat tgattggaac cagttaagat aaactgtgtt tccttttgga cttcatcact
    15421 aatacttttt attgtagata acagctccgg agcatattga aattcatcta ctatataatc
    15481 tgcttgttta tttgcaaata gagagtaggg gtcttctttt gctgctcttc tggtatttag
    15541 gttttccaaa tcaagataat agtaatcggg aaaagtcatc tttgccaaag ttgttttgcc
    15601 tgattgtctt ggtcctgtaa tagttatgat aggaaatcct ttacacagat gaagtaaatg
    15661 gtcttgcatt tctctcttaa ccatctttcg tctcccattt atgtaaattt ggaatctaat
    15721 tccatatttg cataaaatta gggtcgggaa aagctgtcaa ctaaaacttt tcttacaccg
    15781 aaaacgccga gaggagatta aaatgaagaa tgaaaagcga tcataagagg ggaggtcaga
    15841 aaaaataaat aataattgat tttttagaac tacttatcat agacccgatt attaaaatga
    15901 tattggcgtg ccttttttca atttgtaaga aacaaaccag cttttacttg acgccaaacc
    15961 ttcttttcag aattggttca aaacagttaa aaaatatgtt aagaagaaca aaacaacgac
    16021 aaaaaaaaga ggtaactgcc aaaatggaaa taagtaagac atttgaacct aaagacctgg
    16081 aaaagaaatg gtatcagttt tgggaagaaa acggctattt caagccccgc gaagacacta
    16141 accaaaaacc gtttaccgtc ctaatgcccc ctccgaatgt gacaggtatt ttgcatatcg
    16201 gtcatgtttt gaataatact cttcaagatg tcgttgttcg ttatcaccga atgaaaggtg
    16261 aacctactct ttggctacca ggagttgatc atgccggaat cgccacgcaa aatatggtag
    16321 aaaaagaact tgccaaacaa ggaattacgc gtcaggaatt aggtcgcgaa aaaatggtgg
    16381 agaaaatctg gcagtggaaa aatgagaaag gcaatattat tattgatcag ctcaaactct
    16441 tgggagcaag ttgtgattgg gaccgcttac gttttacgat ggatgaaatg ctttcgcgag
    16501 ctgtgaaaga ggtttttgta accctatatg aagaaggact gatttataaa ggcaaatata
    16561 ttattaattg gtgtccgcgt tgtgttactg ccttagcaaa tgacgaagtg gaacacaccg
    16621 atgaaaacgg tcatctttgg tatattcatt atccttttgc tgaaggggat ggctatgtta
    16681 cagtagcaac aacccgtccg gaaacaatgt taggtgatgt tgctgtagcc gtaaacccta
    16741 aagatgaacg ctacagttcc ttaatcggta aagaattgat tttgccttta acggacagaa
    16801 agataccgct aataactgat gaatatgtag atagggattt tggaaccggt tgcgtaaaag
    16861 ttactcctgc tcacgatccc aatgattttg aaattggcat cagacataat ttaccccaga
    16921 ttttagtgat ggatgaaacc ggaattatga acgcaaatgc aggaaaggaa tttcagggtt
    16981 tagaccgcta tgcctgccgg gaaaaagtgc ttaaaatgct taccgaacaa ggtctgctgg
    17041 aaaaaacgga aatacattct tattctgtag gacattgtta tcgctgtgat acggttattg
    17101 aaccctatct ttccgatcag tggtttgtaa aaatggctcc tttagccgaa aaagccatag
    17161 aagttgtgga aaaaggagaa gttaagtttc aaccggaacg ctggacaaag gtctatatgc
    17221 attggatgaa taatattcgg gattggtgta tttcgcgtca aatttggtgg ggacatcgca
    17281 ttcctgtata ttattgtgat cattgttctg agatgattgt agctaaagag atgccggaaa
    17341 aatgtcctaa atgcggagga actaaattta ctcaggattc cgatgtgctg gatacctggt
    17401 tttccagttg gttatggccc ttttccacaa tgggttggcc tgatgaaaca gctgatttaa
    17461 aacgctatct tcctaccaat gtattaatta cagctccaga aattatatac ctgtgggtag
    17521 ccagaatgat aatgagtacc ctgcattttg tagataaaat tccttttgat actgttctat
    17581 tgcatggaac tgtaagagac gaaatcggac gcaaaatgag caagtcctta ggcaattctc
    17641 ccgacccaat tgatttaatt aataccgttg gagccgatgc tttaaggttt agtatgattt
    17701 ttggcacacc caaaggtgcg gatgtaatct tcagtgaaag tattttggaa acgggacgca
    17761 actttgccaa taaaatgtgg aatgcctata ggttcattat gatgaatgcg gaaaatattg
    17821 aaggattacc tgcggaaaat gaattgcaac tggaacttgc cgataaatgg ataatttccc
    17881 gcttgcagga agtgataatt gcagtgcgtg ctcactatga aaatttgcgc tttaacgatg
    17941 ctgctacaga aatctttaag tttatgtggg atgaattttg tagctggtat attgaactta
    18001 gcaaagaccg tttaaatcct tcctcagcac tttcgggcag attaacggct aagtatatac
    18061 ttttgtatgt gatgcaaact ggaatgcgtc tgttgcatcc tattatgcca tttattacgg
    18121 aagaaatttg gcagggtata aaaaaaatct ttccaatgga agaagaagca ctcattattg
    18181 ccactttccc agaaacagat gagacactta aagataacga tattgataat aatatgcgct
    18241 ttgtgcagga aagcattact gccattcgga acctgcggaa acaaataaac ctggctccaa
    18301 atcaggaaat agacatcgtt attcggtttg ccgaaaattc tcagcaggaa ctctttgaaa
    18361 attatatggc ttactttaac cgattggcaa aagtgaaaac cctttcaggg ggagtaaata
    18421 ttgccaaacc taaagctgca attgcttctg tggttcgcaa tattgaaatc tttttgccct
    18481 taagcggatt ggtggatatt gaaagtgaaa aaaatcggct cggaaaacaa attgaaaaac
    18541 taaccaaaga actgagtgga attaaggcaa aacttgccaa tcccaatttc ctgaataatg
    18601 ccaaagaaga agttgttacc aaagagaaag agaaatttat agaagttaat accaaactgg
    18661 aattgctgca aaacctgctt tctgagttat agacatattn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnt tgtacttata tacataactc tttaatatta aatcctgttg
    18841 atcctgtcta tccagttatc tgcgttctat ttttttaatt tcttcttttt ttcttcccat
    18901 ccaattcctg caaccaaaag aagaaccgtt gccagaatac ctactaagct taaagtcaaa
    18961 cttaatttat aggtttcaga gatgaatttc atttccagag tatgttttcc cggaggaata
    19021 attacccctc tcaaaatgtg gtttacaggg taaatttctg tttttttgcc gtctataaaa
    19081 gctttccagc ccgcagggta atagacctca gaaacaacta aaaaggcatt tttatcggta
    19141 gccacatcat atttaacatt gtgcagatca aaataggtct cttttgcaaa gcaactatcc
    19201 ggtttaaaaa cgccatctat cttactttcc acaatagcta aagagcgtgg attaaaagac
    19261 ggttcccgca ttttttgcaa acgggcttca gtttccggaa ttacggatag ggaatctaca
    19321 aaccaggcac gcgggtaagc ggttaaattt tgatagatat tagcacctga gaaactcgta
    19381 aaaacaggtt ttattttgtt caaaagaaaa gcgtagggca gggaatcggg atagataata
    19441 tatttagtag aaagcatatc cagaagaggt gtagggattt ccactccacc tttttcataa
    19501 acaccattca gataacggta aaattcactg taatttttgt cgtccccctg aataattttc
    19561 catacatcat catagcgttt tagtttggca ccgctgtaac catctatggt ttgatgataa
    19621 tatgcccatt ctccggcagt tctaatttgt cccatattat atggatagat acgataattg
    19681 tctttatctt ccaaaaggaa ttcatcaaaa tcctgagcta caaaacgcgc ttctctttct
    19741 tctgccggat aaaggtcttt taaatgcttt cccgtgtaga tatacaggtc tatgaaagta
    19801 atcagagtaa tcaacaaaac caaagcaacc ttcttcagtt ttttggcgct ggcaagataa
    19861 caaattccca gagaaacagt tagcagcaat aaactcataa ttccgctttt ataaagcatt
    19921 ccaggacgca aactcttcaa ttcctccaat ttggcgaggg cattatatct ttgatattgt
    19981 tctatttcag cggcattggt atagggcaga cctttgaaaa tactttgtcc caaaatgaga
    20041 aagagaataa aaaccactcc gcttatccag aaagcggtaa ataacttctt ctgccacttg
    20101 ctgttatttt ccagggaggc aattgtatcc aaacccaaac ctgccaatat aacggctata
    20161 aattgaacca taatcaaggt catagaaggc acccggaatt tattgaacat cggcagatac
    20221 ttaaaaaaga aaccagaaat aacaggagta aaactgccaa agctaagcac taaaaaaata
    20281 gcggaagcaa tccacaaaaa gagggaaaac tttttgtgct tgccaaagag agctataact
    20341 cccaaagcta aaacaactat gccaaagtaa ttataaattt gcgtgaaagg cattgcaccc
    20401 caataattct gatttattcc accaaagaaa tcaggaatga taaaggaaat tatttctaga
    20461 ggtgggaaac tccattgctg agcataactt ttttccagac ctgttgaacc tccacgcatt
    20521 gtgtaatgac tatattccca ggtagaaaga tagggattca tcaccgctaa ggcagttaca
    20581 gcaaaagcaa taatcagcag tccggtccag ttgccgaact ttttaagctg cttagttttt
    20641 attgcctcaa tcaaaccgta aatccaaaac ataccgataa acaaataaag gtaataagtt
    20701 atttgaggat gattttcccg taattgcgta attagaaaag ttgccagcaa gcccaaattc
    20761 aatatatttg gcttttcctt caagcgaaac aaagcccaaa cgacccaggg gatatacatt
    20821 atggcacgaa atttggtatt atgtccaatt tccaacaaac cgacccaatg acaggaaaaa
    20881 gtgaaagcaa tagcggaaaa aaaggcaatc caggggtcca ttttcagttg ccttaaaagt
    20941 aaaaaaatac ctactcctcc aatgaatagc agaaaaatac gccagttgat aattttatcc
    21001 gtcagcttgg ttatattttc cagaaaggga taacgattgg gaaaatgaat catatatgag
    21061 ggcattccgg aaaacatata aggagtccat aaagcattat ctttatgcgt tgcattataa
    21121 tcaattatgg cttttgctgc accctgccat tgaattatgt ccgatgcctg aggtgctttt
    21181 ccttgatagg caacaggaaa atatattaaa ctgagcagga gaaaaagaat tatgaccaaa
    21241 acccagggaa gatgtttggc aaaagcagag ggctcttttt ccctggcagt agttttagca
    21301 ctaggcatag ttttagatgt tttgctgata ttttgcctga ttttctgatt gacatttttt
    21361 ccagccataa tatcctctat ccataatatt ttttacataa caaaataaaa gcggatatcg
    21421 gtcaagaaaa atcccgctac aaccttttct taagaggtaa aaacaatgat ttttgcgttt
    21481 actaaaagaa tctatggcta cgaatgcgat atttatggtc atctaaataa tgccaactat
    21541 ctgcaacttt tagaaggagc acgtagcgaa gcactttatg aaatgaattt agctcagtcc
    21601 gatctccaga aaaaaggagt ccaactcttt gtccattatg tggaaatgag ctttattaaa
    21661 gccattgacc acgatgaact gatcactatt aaaagttgct ttaccgaaat gaacagaata
    21721 aaaggaaaat ggcatcagga aatttataac agtaaaggag aaaaatgctt taacgcagac
    21781 ataacaatcg tttttgcttc agaaggtaaa gcaaaacgac tgccggttga ggtctatgaa
    21841 ctctttatgc actacctgga aaaatagatt aaccagctaa
//
LOCUS       DAOPFL010000022        21727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2551_length_21727_cov_114.064646, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000022 DAOPFL010000000
VERSION     DAOPFL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 21727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21727
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_21727_cov_114.064646"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            835..1305
                     /gene="rimP"
                     /locus_tag="PLG69_02960"
     CDS             835..1305
                     /gene="rimP"
                     /locus_tag="PLG69_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPI42500.1"
                     /translation="MEFYRAQVEEIARKICQEMHLAVYDIEEKMSGKGRIITVYLTKI
                     GGVTLDECATFSNALGKELEIFDLIPERYFLEVSSPGLERPLKLKSHYVSAINEKVAV
                     QFQAGEERKSIMGTLTEVNQDTIVIDDRGSVTEIPFKSIIRAKTVFLAIPKRRD"
     gene            1309..2544
                     /gene="nusA"
                     /locus_tag="PLG69_02965"
     CDS             1309..2544
                     /gene="nusA"
                     /locus_tag="PLG69_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPI42501.1"
                     /translation="MSANMLDALNKLAAIKQLNKDTIQTIILEAVTSTLQKRLEPEAG
                     LQVYIDELAGCVKVKFKSLVVEREEGLGQISLIDARNDYYHNVQLGEYIEKTMTLSEF
                     EPKIVKTVQKIIQDKIRQLEEEKIQNDFNKQKHTIVTGKIKAIDDYGGYIIDTGHVDA
                     LLPVDEQIENEFYRVGDNIKAYVVNIRTQKDGVVIILSRTNPEFVKKLFEAEIPAIFS
                     GEIKIRKIVREPGIRTKVELEAEDPKVDPIVACVGPKGTRIDSLRKELHGEQIDIVLH
                     SDDPEKMIENALGVEGIKRVIIERNHSASVILDEADKLMAIGKQGKNVKLAAKLVGMK
                     IDIYTMAEFEEKMAKERRTVSHITELDGVTPKIAETLKQAGYTSVQDIYTASLEELCN
                     LEGMGQKTAERLKEAAKYF"
     gene            2544..2774
                     /locus_tag="PLG69_02970"
     CDS             2544..2774
                     /locus_tag="PLG69_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HPI42502.1"
                     /translation="MPNKNSKAGHIPIRTCVVCKKKVDQKQLLNFFLTESGIVFDCNR
                     IIQVRKFYLCPVADCFKGLAKWRKSHQKRKIR"
     gene            2771..3100
                     /locus_tag="PLG69_02975"
     CDS             2771..3100
                     /locus_tag="PLG69_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPI42503.1"
                     /translation="MNPKILTLMQFARKAGKLVSGFNACERAIDKKDIFLLILALDSS
                     ERTREKMKKMAANSGISTSVIETGSQAEISSALGLPLTGVFGIKDKNFAGKIMEYWLS
                     DTERRRH"
     gene            3097..5637
                     /gene="infB"
                     /locus_tag="PLG69_02980"
     CDS             3097..5637
                     /gene="infB"
                     /locus_tag="PLG69_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPI42504.1"
                     /translation="MTIRVHELAKELKISTMALKKHLTDLGVNVKSHMSLVEDEVADK
                     IRLKYKEQIDAEKRAERDRKRLIEMRQAAKAQKSAQEAETIKAEEPVPPVAEPVKEVP
                     KKEEPKPVKEPEKETPIPVKEKPVEVKPEEKPHKPAPEKPKAVPKPKPEVIPPKTAAP
                     AKPEPWKKQEPSAPSIKPIPTPPSVEIKKEEKKFGKAKISHEELGEKSKHKKAIISST
                     KKAKQKLIETIEIDEAEISRNIKKTLQKSSKRKKYHREAQYVEDRSNEIVIREFTSVS
                     ELAKIMNVSPSDIISKFFMMGQLVTMNQRLDRDSLEMICDEFKVDYRFEDEYGMDIID
                     KEREQYNNVKEEPRPPVVTIMGHVDHGKTSILDYIRNTNVVAGESGNITQHIGAYQIE
                     INKHKITFLDTPGHEAFTAMRARGANVTDIAVIVISATEGVKAQTREAIDHAKAAGVS
                     IILAINKVDLPEANVDKVIAQLMEIGVYPEQYGGDIPWCRTSVVTGEGILNLIELILL
                     TAEILELKAKRDVPASGVVIEAGMDARMGPFATILMQEGTLHKGDIVVCGAVHGRIRK
                     MENERGAELKVLYPSDVARVYGLSDTPKAGDILNQVDSEKTARNISTERQQIRLEREK
                     YQNKSSLQNIFARIKEEQINTLNIILKTDTDGSAEALADSFQKLSNEEVSVNIIHKSV
                     GGISEADVSLAAASDAIIIGFHVRPSHQARKLAEEEGVQIKLYQVIYDVIDDLQNALE
                     GMLKPKYEEKVIGSAIVKQVFKIKRVGTIAGCQVDKGVIQANCKVRLYRNDVLVTEDT
                     ISSLKHYADDVKEVRAGSECGLTLANFSDIKEDDVLEAYIVNEVNKKL"
     gene            5637..6122
                     /gene="rbfA"
                     /locus_tag="PLG69_02985"
     CDS             5637..6122
                     /gene="rbfA"
                     /locus_tag="PLG69_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPI42505.1"
                     /translation="MKNYRIPRLQAELKKLFNIALSQKLNDPKLAWVQITDVVISKDL
                     HYAKLYFSHYNNPASHDQIRELLIKSSGFLKKQIAGAQIMRTIPELSFYYDDTEDRAA
                     KVDTLLASLRDDFDDEDEEDQDIDIDDILDDDDYFDFDEEDDEEDYDDYEDEEEDEEE
                     E"
     gene            6202..7206
                     /locus_tag="PLG69_02990"
     CDS             6202..7206
                     /locus_tag="PLG69_02990"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPI42506.1"
                     /translation="MQNIRKELVELIAKAENILLTTHISPDGDGYCSALALQRIISFL
                     GKSSLLVTDDDDLSRYNYLQDGKSREKRFSELKEEEQNFSLAIVLDCNSYDRLGERCV
                     LVEKAEKIIVLDHHEAENGLIKAELSYIEPSFACVGEIIFSLLETAINKMPVSDRLFV
                     CNCLYTTILNDTNNFVNANTDTDVFIFASKLVNLGVKPNEQYRYFFLQHSAEELRYIG
                     ETLSTIELHYQRKILFMYSTLAMSKKNNIDPESIMNATRWVQGIIGIEVITYLREEKE
                     GVYKISLRSETIDVNRIAVRYGGGGHKQAAGCYFYGTLAEAKDKLLKDVINALECYNG
                     "
     gene            7181..8062
                     /gene="truB"
                     /locus_tag="PLG69_02995"
     CDS             7181..8062
                     /gene="truB"
                     /locus_tag="PLG69_02995"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPI42507.1"
                     /translation="MPLNAITDKAFLAIDKPSGITSFAVIRRLRKITGSKKIGHTGTL
                     DPFATGLLICCLGSYTRLAGLLEEKDKTYLARMKLGIKTATGDPEGDIIQTAPIPEFV
                     SKLDILAKKARQLTELPVPAYSAVKVNGKRAYELARKGEKFNLPKRPVQIKEFEFLPW
                     EPNKSDELSYRCKVSKGTYIRALSEWLAEQLNTIACTTFLRRESIGNITLQDAVSLDD
                     LTNDNWFRYQLSYKKVFPDYTCLKLNADDFAKVMNGMDIAHSNENNEKYILLKEENIC
                     AIGQRMNNYIHPLRVLK"
     gene            8059..8187
                     /locus_tag="PLG69_03000"
     CDS             8059..8187
                     /locus_tag="PLG69_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42508.1"
                     /translation="MNKRKNEVLVVYCFPNYKVLDNNYNSEIMNFVWWTRTRYENE"
     gene            8180..9085
                     /locus_tag="PLG69_03005"
     CDS             8180..9085
                     /locus_tag="PLG69_03005"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPI42509.1"
                     /translation="MSKKSVLTIGTFDGLHLGHQKVLKKVVEIAMAEGLQSVVISYKD
                     HPAFVLKTHPMPKMLCPAEIKLQEMKKMGIERIELLDFTLELAKTPPLKFLKDYIIPQ
                     FQPEVIVMGYDSHFGHLRQGNYDFLCRHSEELGYRVEYVEPLLFEGKPISSSSIRNLL
                     LSGKIEAANNLLGRPYRLIGKVGHSNSKGRKLGFPTANLNLLCPHQLIPKSGIYLSKV
                     IWQENVFFGLTNIGCSPTVKNTGVIEIETHILDFQDNLYGQIMELDLLSYLREEKMFK
                     NVDELKTAIQNDIALARVMLKEFSG"
     gene            9134..11065
                     /locus_tag="PLG69_03010"
     CDS             9134..11065
                     /locus_tag="PLG69_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI42510.1"
                     /translation="MRKVNKPTVHLAKRNLLFPLAVLFFLLLPVFFSAVSPLQVVPGS
                     GIEDIEDSKLYHFRYHNLVDDYHLSGPKIWAVRFNFKDAYPIADSASFTLNSFKIYSP
                     YPNLEMRVSLWNEQYGGNNELNYFPGVIIPGGNWQNITLSTSPATITFPEIQNPLRII
                     WLVMDFTTPLADKYMSASEGSGKNSFYYNNTVQGNEFWQSLFTAGFKCELRVSAIGDF
                     NLLHTDLELLNFSLPENILPGSTVHPSVTVYNHSQYPVSDTLEVNFSDPLLELNQTLQ
                     IPLLNINPRDSLFTVSSEGITFSREPTQIKVTLAFKNHPNIIFSARYYNVFGETGSSH
                     LVEYFRRYTFGFSDIPQEASEVHHLLYFPNLADNLSNLFAVQRFNYYQFNSLPKTVVD
                     GYKKFHLQIDANSEELVSAISAAQAKRSFISRSSSNISMPDPETPENLLLNIKLYNDR
                     TTLYEWTASSALNPKFFAGIFEENEFAGGELYSLKRWLVFEGSFSGTLNKRDSVEIEL
                     ILNTTDLNPSKTYRVYYWLQNPFNSGGQIYYAQYVSLGQSWIVSNSDEYISQPVISVY
                     PNPLVKGNALKISASVFPAVFTVYNIKGQKVFSTGKVFSEISIPADIFPASGIYFLRC
                     EMQDRDKTHKQIQKISIIK"
     gene            11075..12028
                     /locus_tag="PLG69_03015"
     CDS             11075..12028
                     /locus_tag="PLG69_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPI42511.1"
                     /translation="MAEHISIAEMQQKVNQEISGLYLVAEKELREGKNDFYLRLKLQD
                     KTGTISANVWKDAVKHSDQFDTGDIISIQGTVVNYKGQIQLSLSQLRFADKSEYNIED
                     YLTRSKIEPEVLCDRFFEFVDKVHQPYLNKLLHLIFDDKEFFTQFLKAPAAKNWHHNY
                     INGLIEHTVSVAALCEFASTLYPVNYDLLITGALLHDVAKVQEYDHRRNIDFTDEGRL
                     IGHLALSDQMVCENAAKILGFPEELLLHLRHLILSHHGEYEKGSVRLPQTLEAIVLHH
                     CDNLDAQATGVAQLIAAIPQNAVWSEYDKLNNRYYKIYRPV"
     gene            12133..12918
                     /locus_tag="PLG69_03020"
     CDS             12133..12918
                     /locus_tag="PLG69_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPI42512.1"
                     /translation="MFQTVIIASGSKGNCVLIQTENTALLLDAGISMHRIMECLESYH
                     IESAKINGVLVSHEHSDHIKSVGAISRKLKIPVYINRPTLSSCAEHLGDVQDRIVLFN
                     TGNSFTIGDIIIEPFVSTHDAAESCNFVFYPVSEPKRKLGVATDLGYPTQLCLLKLSG
                     VSTLILESNHDEKMLMEGPYDWKLKQRIRSLQGHLSNVQAVGLISALIHPGLKNLILA
                     HLSEINNLPSLAEQTMREYLNSIRSDIKLFVAEQNRPTPPIEI"
     gene            complement(13356..13547)
                     /locus_tag="PLG69_03025"
     CDS             complement(13356..13547)
                     /locus_tag="PLG69_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42513.1"
                     /translation="MVIIIRHKTRNFIRGYKFYCSNVVPAKAGIQGIIKITYASFSPG
                     SKPVVSICRSYGTLCRKKR"
     gene            complement(14354..16723)
                     /gene="priA"
                     /locus_tag="PLG69_03030"
     CDS             complement(14354..16723)
                     /gene="priA"
                     /locus_tag="PLG69_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPI42514.1"
                     /translation="MHYYVIHLPLEIPKELIYCSSAEIVEGARVIVNLSGRLWTGICG
                     AKTRPEEDKDIRYKAVIEVLDTVPVLSPELMKLGYWMADYYHCSVGKALFAMLPARLL
                     PEMEANVIWLSENVPEPFIPLKKAIDKSENPVLKTLRKELTSYPLYKRIEEGEELGLL
                     KIERRLNHKDKPRTVNFIIRLQPTLEEDTLPLKQREAWEKIKEEEPSFPMSNISSTIS
                     YSAIKALVKKGFIKIEPRRFDPESISFENSSPLKQIALNHAQVEAIKDITEGFGSFNV
                     NLLYGITGSGKTEVYIELIRHYLSVNKTVIFLIPEIALTPQMVDRFQSNFGQILAIQH
                     SQLTEKDRLIQWQKIKSGEKRIVIGARSAVFAPLPDLGLIIVDEEHEGTYKQESAPRY
                     QGRDLAIVRAQIQNAQVILGSATPALESWYNALNGKYKLQKIESRPLDYCLPDVQVVD
                     LRDEYEQSLISAPLLEAIDKRLQRKEQVILFQNRRGYSSFMQCLKCGKLITCKNCEIS
                     MSYHRDREEMQCHYCGNTIPSPRKCPYCGSYSFAYGAPGTQKIEQNLKILFPEAKILR
                     MDSDSARKRDTYKSMYNRMKNKEVDILLGTQMISKGLDFPLVTLVGIVMADISLNLPD
                     FRAAERTFQLLTQVAGRSGRRDKAGEVIIQTYNPQHYAIESASQQNYIRFATEELGYR
                     KKLYYPPYYRLARILFQSTDLETLKKEMSNMREVLYFLRKQFPMPQLLLLGPSVAPFS
                     KISSYYRYHLIFKAQTVKILQNAIRLFNSLYNCPSNIRKQIDIDPNTLM"
     gene            16848..19418
                     /locus_tag="PLG69_03035"
     CDS             16848..19418
                     /locus_tag="PLG69_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPI42515.1"
                     /translation="MRKKVLTAFMLGFMLISTLMGENISSPVLSQTKSDTLYLQDFSL
                     LSEDDFRELEDLLKEQQLNPQDLNFEKDWDLSTWGKSKSFLRSLQEPYYGLGLMQEIR
                     EAANKANTDEGFAFLVDLLYKNATDGAGSFPLYLNYKDLYQQEFNKKVKQPKDLFRFY
                     EKALKDIIPVLANAYSHYNLDLWNKYVFYLFVALRENEDSEAYNAYFNKHNIPLWEEI
                     EDQEIKELMELFQEADVLKAAMQFLALCDVIKNEAGKLNYTNKKPIIQECEFGTMVIG
                     TEKDDVYNAKMFKKPLILLIEPAGNEKYTMTLATGAKNYAYLLIDYKGNDSYVNPDIG
                     SLFFALSGLGISYDLEGNDIYRTGDFSFAGMMGIQLHRDFQGTDIYESGFFSQGAAIR
                     GLSILQDMQGNDIYSATCLAQGFGSIRSAGVLLDFDGADNYLLGGKYFHSPLMPNDYL
                     TLGQGVGFGLRPDFAGGLGLLFDKNGNDRYLGGVYAQGVGYWYALGMLIDEAGNDVYN
                     AVYYPQGSGIHLASGFLFDGSGDDAYYSRHGPGQGAGHDWGLGIFIDAKGNDAYSIEG
                     GNGLGLSNSVGIFIDKGGNDRYERNNPQNYGNAVYSRATGGIGLFLDKDGKDSYPDTL
                     MAEGTTWKKGTYGIGRDLDSRNLMSSSTTDSETKEPAEIPMLPPPSPDEPIADIFSAA
                     AEWEVGNAIERVKKAREIMLARADEAIDYVIKNKLDTKSGLEYRALEVLVKENEQFKQ
                     LLFDYTDDIDSLKAKNALSLIAGVGDSTLIEPLRKHLNNGKYITTCLSLLGSIKSAES
                     VDLLFQYAFHPSERYRYIVARSLKQIGTPEAHNCLKQMANDNSFLVKTLVRKWEEEQQ
                     "
     gene            19415..20056
                     /gene="rsmG"
                     /locus_tag="PLG69_03040"
     CDS             19415..20056
                     /gene="rsmG"
                     /locus_tag="PLG69_03040"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPI42516.1"
                     /translation="MTEEKQIFEDYLRFLGLEDIDHLMARFEHYLHLLYNANRKVNLV
                     SRNIPLERYWVQHFLDSIVALECLDFTDKTVLDFGSGGGLPGIPLKLAVPDFNLTLLD
                     SVQKKARVLREFVDALSLSATNVEASRLEDYAYVAKRPSYDFIVCRAVALEERYFSPL
                     RRLLKPSGMVVFYKAQKLDDVQVLKYEILLDKDYPALGQRKLIGIKQRDLLFH"
     gene            20081..20845
                     /locus_tag="PLG69_03045"
     CDS             20081..20845
                     /locus_tag="PLG69_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPI42517.1"
                     /translation="MPKVITVVNQKGGVGKTTTAVNLSAGLAVLEKRTLLIDFDPQGN
                     ATSGVGIDKDKVELQIYDALIGRAPIEKTILSTATKNLFCIPGNINLTGAEIELVHEF
                     AREQKLKEALQPILNTFDYIIIDCPPSLGLLTVNAMTAAMEVLIPIQCEYYALEGVSQ
                     LLTTIRLIQKNLNPGLNILGVLLTMFDKRVNLSLQVAKEVHRYFKEKVFRTIIPRNIK
                     LTEAPGFGKPIFLYDIRSPGAMSYLNLANEVINRSE"
     gene            20857..21714
                     /locus_tag="PLG69_03050"
     CDS             20857..21714
                     /locus_tag="PLG69_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPI42518.1"
                     /translation="MNEHLGRGLFALIPDNDREQTPQLGITTLPIASIKPNRYQPRKT
                     FDPEKMAELTESIKENGIIQPLIVTKTSSSEYELVAGERRLEAAKQAGLEKVPVVIRS
                     VSKKEQLQLALIENIQREDLNPIEEAIAYNTLVEDFGLTHNQISEIVGKDRATVTNSL
                     RLLKLPEEVKQMLVSGELNSGLARTVLSVPPELQNSFAEYIIKYNLTVRQAEEKAKTF
                     SLANKEEPEKAKTNARTKELERELSNLFCLKVKVQENNGKGKITLVYKSPEELEAFRT
                     ILNKIRQEK"
BASE COUNT         6926 a         3783 c         4549 g         6469 t
ORIGIN      
        1 gttgaggtct atgaactctt tatgcactac ctggaaaaat agattaacca gctaacatat
       61 aatgtgaaaa agtggaaagg taaaacacgg ggtagacatt gaaaacatag ccataaaata
      121 ggttgagagg gttgagaagg tttagaaggt ttagagggtt tagaaggttt ttctggttta
      181 ggaaattgtg aaagttgaaa acccggatgg cgatacaatt atagcgatgg tggcgtaagc
      241 ccctcggata aaggatttaa taaccctatg cttttgctct ctgccctctg ccaaaaaggg
      301 ggttgtgaga caactccgtc gttagtatct gtcgttccta cggaattttt tctaccacat
      361 ataagttagg attatggaaa tagtatgcat tactttagca ctgctcactc attactgata
      421 tcatccgtaa tgggtgagca atgggtaagc aatgcaatta aggaagaact gatttttttg
      481 aagattttgc ttgacaaata tgagcagaga tttttcaaag aatttatgta aaaaaaccga
      541 taccgaaaat cggttgaaag ggaatagtaa ccgttcaaga cggaaataga tagctacatt
      601 gatagctatt ttttttgagg taaattgggt atggggatga gataatatta ggcaaagtat
      661 gatggattat gagagaggaa taaggaaata gagtgtttga ctggatggtt gatgttcaca
      721 tcaacctaaa gtgaaagatt ggaaaactaa atcatagtta gtcataaaag aataaagagg
      781 ataagataga acgacgagga tttcgttcat ccggaaaaga atgaggtaaa aaagatggaa
      841 ttttacagag cacaggtaga agagatagcc aggaagattt gtcaggaaat gcatcttgca
      901 gtgtatgaca ttgaagagaa gatgtctggc aaagggagaa ttataactgt ctatcttact
      961 aaaattgggg gagttacatt ggatgaatgt gctaccttca gtaatgcttt gggcaaggaa
     1021 ctggagattt ttgacctcat tccggaacgc tattttttgg aggtttcttc tccagggctg
     1081 gaaaggcctt taaagctaaa aagtcattat gtgagcgcca ttaacgaaaa agtagcggtt
     1141 caatttcagg caggagaaga aaggaaatcc attatgggca cattaacaga agtgaatcag
     1201 gatacaatag ttatagatga tagaggatca gttacggaga ttccgtttaa atccataatt
     1261 cgtgccaaaa cagtattttt agctataccc aagaggagag attaaacaat gagtgcaaat
     1321 atgcttgatg cccttaataa actggcggca attaaacagt tgaataagga taccattcaa
     1381 acaatcattc tggaagccgt aacatccact ctgcaaaagc gtttggaacc ggaagcaggt
     1441 ttgcaagttt acattgatga attagccggt tgtgtgaaag taaagttcaa aagcttggtt
     1501 gtggaaaggg aagaagggtt gggtcaaatt tctttgatag atgcacgtaa tgattactat
     1561 cataatgttc agctgggtga atatattgaa aaaacgatga ctctgtctga atttgagccg
     1621 aaaatagtga agacggtgca gaaaataatt caggataaaa tccgccaact ggaagaggaa
     1681 aaaattcaga atgacttcaa taaacagaag catacgattg taaccgggaa aattaaggca
     1741 atagatgatt atggcggtta tattattgat accgggcatg tggatgcttt attgcctgta
     1801 gatgaacaga ttgaaaatga attctatcgc gtgggtgata atataaaagc gtatgtagtg
     1861 aatattagaa cccagaaaga tggagtagta ataattcttt cccgcaccaa tccggaattt
     1921 gtgaagaaat tgtttgaggc agaaattcct gctattttca gcggagaaat taaaataaga
     1981 aaaattgtgc gcgaaccggg aattagaact aaggtagaat tggaagcaga agacccgaaa
     2041 gttgatccta ttgttgcctg tgtaggaccc aaaggaaccc gcattgattc tttgcgtaaa
     2101 gaattgcatg gggaacaaat agatatcgta ttgcacagtg atgacccgga aaagatgatt
     2161 gaaaatgccc tcggggtgga aggcataaaa agagtaatta ttgaacgcaa tcattctgcc
     2221 agcgtgattt tagacgaagc agataaactg atggcaatag gtaaacaggg gaaaaatgta
     2281 aaacttgccg ccaaactggt gggaatgaag atagatattt atacaatggc agaatttgaa
     2341 gaaaaaatgg caaaagaaag gagaaccgtt agtcatataa ctgaactgga tggtgttact
     2401 cctaaaattg cagaaacctt gaaacaagcc ggttatacaa gcgttcagga tatctatacg
     2461 gcttccctgg aagagctttg taaccttgaa ggaatgggac aaaaaacagc tgaacgcctt
     2521 aaagaagcag cgaagtattt ctaatgccta ataaaaatag taaagcggga cacattccta
     2581 tccgcacttg tgtagtgtgt aagaaaaagg ttgaccagaa acaactgctc aatttctttc
     2641 tcaccgaaag cggaattgtg tttgattgta acagaataat ccaggtgcgc aagttctatt
     2701 tatgtcctgt tgccgattgt ttcaaaggac ttgccaaatg gcgtaaaagt catcagaaaa
     2761 ggaagataag atgaatccga agattcttac tctgatgcag tttgccagaa aagcaggcaa
     2821 acttgtttcg ggttttaatg cctgtgaaag agctatagat aagaaggata tttttctgtt
     2881 gattctggca ttggacagtt ctgaacgcac cagagaaaag atgaaaaaaa tggctgcgaa
     2941 ttcgggtatt tcaacttctg ttatagaaac cggaagtcag gcagaaatca gctctgcttt
     3001 gggtttgcct ttaacaggtg tgtttggaat aaaagacaag aattttgccg gtaaaattat
     3061 ggaatattgg ctctctgata cagagaggag gagacattga cgattagagt tcatgaatta
     3121 gccaaagagc tgaaaatcag cacaatggct ttaaaaaaac acctcaccga tttaggagtg
     3181 aatgtaaaaa gccatatgag tttggttgaa gatgaggttg cagataaaat acgcttaaaa
     3241 tataaggaac aaatagatgc agaaaaacga gcggaaagag atagaaagcg tctgatagaa
     3301 atgcgtcagg cagctaaagc gcaaaaatca gctcaggaag cagaaactat aaaggcggag
     3361 gaaccagttc ctcctgttgc agaaccggtt aaagaagttc ctaaaaaaga agaacccaaa
     3421 ccagtaaaag aaccggaaaa ggaaacaccc ataccggtta aagaaaaacc ggtagaagtg
     3481 aaaccagagg aaaaaccgca taaaccagct cccgaaaaac ccaaagctgt tcctaaaccc
     3541 aaaccggaag ttattcctcc taaaacagca gctcctgcca aaccggaacc ttggaaaaag
     3601 caagaaccga gtgctccttc tataaaacct atacctactc ctccatctgt ggaaattaaa
     3661 aaagaagaga aaaaattcgg taaggcaaaa atcagtcacg aagaactggg tgaaaaaagc
     3721 aaacataaaa aagccattat ctccagcacc aaaaaagcca aacaaaaact aattgagacc
     3781 atagagattg atgaagcgga aatttcccga aatataaaaa agaccctgca aaagagctcc
     3841 aaacggaaaa aataccatcg ggaagctcaa tatgttgaag atcgcagtaa tgaaatcgtc
     3901 attagggaat ttacttcggt tagtgagctt gctaaaatta tgaatgtttc cccttcagat
     3961 attatctcca aattctttat gatgggtcaa ttggtaacaa tgaaccaacg cttggacagg
     4021 gattctctgg aaatgatctg tgatgaattc aaggttgatt accgatttga agatgaatac
     4081 ggaatggata ttatagacaa ggaacgcgaa caatataata atgtaaaaga agaaccccgt
     4141 cctcccgtag ttactattat gggtcatgta gatcatggga agacctctat tttggattat
     4201 atccggaaca caaatgtggt tgcaggtgaa tcaggaaaca taacgcaaca cattggagct
     4261 tatcagattg agataaataa gcataagatt acttttttag atactccggg gcatgaagca
     4321 tttacagcta tgcgggcaag aggagctaat gttacagata ttgccgttat tgtaatttcc
     4381 gctacggaag gcgttaaagc tcaaacaaga gaagcaattg atcacgctaa agcagccggg
     4441 gtttctatta ttcttgccat caacaaagtt gaccttcccg aagctaatgt agataaagtt
     4501 atagcccaat taatggaaat aggagtttac ccggaacaat acggaggaga tatcccttgg
     4561 tgtagaacat ctgttgtaac tggagaaggt attttaaatc tgattgaact gattctttta
     4621 acagcagaaa tactggaact gaaagccaaa cgcgatgttc ccgcttccgg agtagttatt
     4681 gaagcgggaa tggatgcccg aatgggtcct tttgccacta ttttgatgca agaaggaact
     4741 ctgcataaag gtgatattgt tgtttgcgga gctgtgcatg gcagaatccg caaaatggaa
     4801 aatgagcgcg gagcagaatt aaaagttttg tatccttctg atgttgcaag agtttatggt
     4861 ctttccgata ctcctaaagc aggagacata ctcaatcagg tagatagcga aaaaacagcc
     4921 cgaaacatta gtactgagcg tcaacaaata cgattggaac gggaaaaata ccagaacaag
     4981 agttctctcc agaatatttt tgcccgcatt aaagaagaac aaataaatac gctgaatatt
     5041 attcttaaaa ccgatacaga tggctctgcg gaagcattgg cagatagttt tcaaaagctt
     5101 tccaacgagg aagttagcgt taatattatt cataaaagcg taggtggaat cagtgaagcg
     5161 gatgtttctt tagctgctgc ttcggatgcc atcataattg gattccatgt gcgtccttca
     5221 catcaagcgc gtaaactggc tgaagaggaa ggagtccaaa ttaaacttta tcaggttatt
     5281 tatgatgtta ttgatgacct tcagaatgca ttggaaggaa tgctaaaacc gaaatatgaa
     5341 gaaaaggtta ttggtagcgc aatagtgaaa caggtgttca aaattaaaag agtgggaacc
     5401 atagccggat gccaagtaga taaaggtgtg attcaggcaa attgtaaagt ccgtctttat
     5461 cggaatgatg ttttggtaac ggaagataca atcagctcct taaaacatta tgctgatgat
     5521 gttaaagaag tccgtgccgg ttcagaatgt ggattaacct tagccaattt ctccgatatt
     5581 aaagaagacg atgtattgga agcatatatc gttaatgagg ttaataaaaa actgtaatga
     5641 aaaactatcg tattccccgc ttacaggcag aattgaagaa actcttcaat attgctcttt
     5701 cccaaaaatt gaacgacccc aaactggctt gggtgcagat aaccgatgta gttatctcca
     5761 aggatttaca ctatgccaag ctatattttt cgcattataa taaccccgcc agtcatgacc
     5821 aaatcagaga actcttgatt aagtcatccg gctttttaaa aaaacaaata gcaggagcac
     5881 aaattatgag aacaatcccc gaattatctt tctactacga tgacacagaa gatcgtgccg
     5941 cgaaagtaga tacgctgctg gcatcgctca gagatgattt tgacgatgaa gacgaggaag
     6001 accaggatat tgatattgac gatattctgg atgatgatga ttattttgat tttgatgagg
     6061 aggacgacga agaggattac gacgattacg aagacgaaga agaggatgag gaagaggaat
     6121 aatttcgctg tttctcacaa acttacaata atatgaaaag gataaatcgc ccttagtggc
     6181 gattttttct ttggtgaaag catgcaaaat attagaaaag aattagtgga gcttattgcc
     6241 aaagccgaaa atattttact tacaactcat atcagtcccg atggagatgg ttattgttcc
     6301 gctctggctt tacaaaggat tatttccttt ttaggcaaaa gttctttact ggtaaccgat
     6361 gatgatgacc tgagccgtta taactacctg caggatggaa aaagccggga aaagcgtttt
     6421 tccgagttaa aggaagaaga gcagaacttt tctttggcaa ttgttttgga ttgtaattct
     6481 tatgataggt taggtgaaag atgcgttctg gtagagaaag cggaaaagat aatagttctg
     6541 gatcatcatg aagcggaaaa cggtttaata aaagcagagc taagttatat tgaaccttct
     6601 tttgcctgtg taggagaaat tatcttttcc ctgctggaaa cggcaattaa caaaatgcct
     6661 gtttccgatc gcttatttgt gtgtaattgc ctttatacta caattttgaa cgataccaac
     6721 aactttgtta atgccaatac cgatacggat gtttttatct ttgcctcaaa attggtgaac
     6781 ttgggagtta aacccaatga acaatatcgg tatttctttc tgcagcattc tgcagaagaa
     6841 ctgcgttata taggtgaaac cctttctaca attgaattac attaccagcg taagatatta
     6901 tttatgtatt caaccttggc aatgagcaaa aaaaataata ttgaccctga aagcataatg
     6961 aatgcaacgc gttgggtgca aggcataata gggattgagg taataactta cttaagggaa
     7021 gaaaaagaag gggtatacaa aatcagtttg cgttctgaga caatagatgt gaatagaatt
     7081 gctgtgcgtt atggtggagg aggtcacaaa caggcagccg gatgttattt ttatggaact
     7141 ctggcagaag ccaaagataa attgttaaag gatgttatta atgcccttga atgctataac
     7201 ggataaagca tttctggcta tagataagcc gtctggaata acttcctttg cagttatcag
     7261 gcgtttgcgt aaaattaccg gaagcaaaaa aatcgggcat accggaacac ttgatccttt
     7321 tgctacgggt ttgctaatct gttgtttggg ctcttatacc cgtttagcgg gtttattgga
     7381 ggaaaaagat aagacctatc ttgccaggat gaaattgggc ataaaaactg caaccgggga
     7441 tcccgaaggc gatattatac agacagctcc cattcctgag tttgttagta aacttgatat
     7501 tttggcaaaa aaagcaagac aattgaccga acttcctgtg ccggcttatt cagctgtaaa
     7561 agtcaacggg aaaagagctt atgaacttgc ccgcaaaggt gaaaagttca atctgcctaa
     7621 gcgtcctgtt caaattaaag aatttgaatt tttaccttgg gaaccgaata aatctgatga
     7681 actaagctat cgttgcaaag ttagcaaagg aacttatatc cgggctttaa gtgaatggct
     7741 ggcagaacaa ttgaacacaa ttgcctgcac aacttttcta cggagagaaa gcattggcaa
     7801 tataactttg caggatgcgg tttctttgga tgatttaact aatgataact ggtttcgcta
     7861 tcagcttagc tataaaaagg tttttcctga ttatacctgc ctgaagctga atgcagatga
     7921 ctttgccaaa gtgatgaatg gtatggatat agctcattct aacgaaaata acgaaaaata
     7981 cattttgctt aaagaagaaa atatctgtgc tattggtcaa agaatgaata actatattca
     8041 cccgcttagg gtgctaaaat gaataagagg aagaacgaag tcctcgttgt ttattgtttt
     8101 ccgaattata aggttttgga taataactac aatagcgaaa taatgaattt tgtgtggtgg
     8161 acgaggacaa ggtatgaaaa tgagtaaaaa atcggtctta actattggca cttttgacgg
     8221 cttgcatttg ggtcatcaga aagtgcttaa aaaagttgta gaaatagcaa tggcagaagg
     8281 tctgcagagt gtggttatca gttataaaga tcatcctgct tttgtgttaa aaacgcatcc
     8341 tatgcccaaa atgctttgtc cggcagaaat caaactgcaa gaaatgaaaa aaatgggtat
     8401 agagagaatt gaactattgg attttacttt agagctggca aaaacccccc ccttaaaatt
     8461 tttgaaggac tatattattc cccagtttca gccagaagtt atagttatgg gttacgattc
     8521 tcattttgga cacttgcgtc aaggtaacta tgattttctt tgtaggcatt ctgaagaact
     8581 gggttaccgt gtggaatatg tagaaccctt gctttttgaa ggtaagccaa tcagcagcag
     8641 cagtatcaga aatttactac tcagcggtaa aattgaagca gccaataatt tattgggaag
     8701 accctatcgt ttgataggca aagtaggaca cagcaattcc aaagggagaa aacttggttt
     8761 tcctacggca aatttgaatt tgctttgtcc gcatcaactt attcctaaaa gcggaattta
     8821 cctttctaaa gtgatttggc aggaaaatgt atttttcggg ttgacaaata tcggttgcag
     8881 tccaacagtg aaaaatacgg gagttattga aattgaaacc catattcttg attttcagga
     8941 taacctctat ggtcaaatta tggagctgga tttactcagt tatcttagag aggaaaagat
     9001 gtttaagaat gtagatgagc ttaaaacagc catccaaaat gacattgcct tagcaagagt
     9061 aatgctgaag gaattttcgg ggtgaactat cttacttatg gactgcccaa ttccaatttg
     9121 gcagatagga aaaatgcgga aagttaataa acctaccgtt catttagcaa agaggaattt
     9181 gttatttcct ttagccgttt tgtttttcct gttgctaccg gtgtttttct ctgcagtttc
     9241 gcctttgcaa gttgttccgg gaagtggcat tgaagatata gaagatagca agttatatca
     9301 ttttcgttat cataatttgg ttgacgatta tcatctttca ggacccaaaa tctgggcagt
     9361 gcgctttaat ttcaaagatg cttatccaat agccgattct gcctctttta cgctgaatag
     9421 ttttaaaatt tattctccct atcccaatct ggaaatgcgg gtatctttat ggaatgaaca
     9481 gtatggggga aacaacgaat taaactattt tccgggggtt attattccgg gtggtaactg
     9541 gcaaaatatt actctttcta caagtcctgc tactattacc tttcctgaaa ttcaaaatcc
     9601 tttgcggatt atttggctgg tgatggattt tactacacct ttagccgata aatatatgag
     9661 cgcttcggaa ggaagcggaa agaacagttt ttattacaat aacacagttc agggcaatga
     9721 attctggcaa agtttgttta ctgccggttt taaatgtgaa ttacgagtaa gcgcaattgg
     9781 agattttaat ttattacata ccgatttgga actgcttaat ttttctcttc cggagaatat
     9841 tttgcctggt tccaccgttc atccttccgt tacggtttac aatcacagtc aatatcctgt
     9901 aagtgacact ttggaagtta atttttccga tcctttgctg gaattaaatc agaccctcca
     9961 aattccttta ttaaacataa atcccaggga ttctcttttt acagtaagtt ccgaaggcat
    10021 tactttcagc cgggagccaa cccagataaa ggtaactctt gctttcaaaa atcatcctaa
    10081 tatcattttt tctgcccgtt attataatgt ttttggggag acaggaagtt ctcatttggt
    10141 agaatatttc cggcgttata cttttggctt ttccgatatt cctcaagagg catccgaagt
    10201 tcatcatctg ctctattttc ctaatctggc agataatctt tccaatctat tcgcagtgca
    10261 gaggttcaat tattatcagt tcaattcatt gcctaaaact gtagtagatg gctataaaaa
    10321 atttcatctg cagattgatg ccaattctga agaacttgtt tctgctattt cagccgcaca
    10381 ggcaaaaagg agctttattt cccgcagcag cagtaatatc agtatgcccg atccggaaac
    10441 acctgaaaat ctattgttga atataaaact ctataatgat agaactactt tatatgaatg
    10501 gacagcaagt tctgctctca atcccaaatt tttcgccggt atttttgaag aaaatgagtt
    10561 tgcaggcgga gaactctatt ccctgaaaag gtggcttgta tttgagggtt ctttcagcgg
    10621 aactcttaat aaacgggata gtgtggaaat tgaattaatt ttgaatacta ccgaccttaa
    10681 tccctcaaaa acctacaggg tttattactg gttgcagaat cctttcaaca gcggaggtca
    10741 gatttattat gctcaatatg tttccttggg gcaatcctgg atagtttcca attctgatga
    10801 atatatatct caacctgtta tttccgttta tcccaatcct ctggtaaaag gaaatgcgtt
    10861 aaaaatatcc gcttctgttt ttcctgcggt ttttacggtt tataatatta aggggcagaa
    10921 ggtctttagc actgggaagg tctttagtga aatatctatt cctgccgata tttttcctgc
    10981 ttccggtatc tatttcctgc gttgtgaaat gcaggataga gacaaaacac ataaacaaat
    11041 ccaaaaaata agtataataa agtgatgtaa cattatggca gaacatattt ctatagctga
    11101 aatgcaacaa aaagtaaatc aggaaatcag cggtttgtat ttggtagccg aaaaagaact
    11161 ccgcgagggg aaaaatgact tttatctgcg gttaaaactg caggataaga ccggaaccat
    11221 ctctgccaat gtatggaagg atgcagttaa gcattcagac cagtttgata ctggcgatat
    11281 tatcagcatt caaggcacgg ttgtaaatta caaagggcag attcagcttt ctctatctca
    11341 gcttcgtttt gccgataaat ccgaatataa tatagaggat tatctaacgc gcagtaaaat
    11401 tgaaccggaa gttctttgcg accgtttttt tgagtttgtg gataaagtgc atcagcccta
    11461 tctgaataaa ttgcttcatc ttatctttga cgataaggag ttctttactc aattcttaaa
    11521 agctcctgca gctaaaaact ggcatcataa ttacataaac gggcttattg agcataccgt
    11581 ttcagttgct gccttatgtg aatttgcctc cactctttat cccgtaaatt acgatttgct
    11641 gataaccgga gctttattgc acgatgtagc caaagttcag gaatatgacc accgcagaaa
    11701 tattgatttt acggatgaag gacgccttat cgggcattta gctctttcgg atcaaatggt
    11761 ttgtgaaaat gctgccaaaa ttttaggttt tcccgaagaa ttattactcc atttacgcca
    11821 tttaattctg tctcatcatg gtgaatacga aaaagggtcc gtgcgtttgc ctcaaaccct
    11881 ggaagccatt gttttacacc attgtgataa tctggatgct caggcaacag gtgtggcaca
    11941 attgattgct gcaattcctc aaaatgctgt ctggagcgaa tatgacaaac tgaataaccg
    12001 ctattataaa atttaccgac ctgtttaaaa tttatgttat ttaagagcga aagttctttg
    12061 ttcttcttgc gaaagcggaa atccggataa attttaacag aacagctgaa tattcaatag
    12121 ggaaattatg ttatgtttca aaccgtgatt attgccagtg gcagtaaagg aaattgcgtt
    12181 ttaattcaaa ctgaaaatac cgctctcctt ttggatgccg gaataagtat gcacagaatt
    12241 atggagtgtt tggaatcata tcacatagaa tcagcaaaaa taaatggcgt tctcgtaagc
    12301 catgaacatt ccgatcatat caaaagtgta ggagctattt cccgtaaact gaaaattccc
    12361 gtttatatta atagacctac tctttcttct tgtgcagagc atttgggcga tgtgcaggat
    12421 aggatagtgc tctttaacac gggcaacagt tttaccattg gagatataat tattgagccg
    12481 tttgtttcta ctcatgatgc tgccgagagc tgtaatttcg ttttttatcc cgtttctgag
    12541 cccaagcgta aattgggtgt ggctacagac ctgggctatc caacgcaatt atgtctgcta
    12601 aaattgagcg gtgtaagcac tttaatcttg gaaagtaatc acgacgaaaa aatgctgatg
    12661 gaaggacctt acgactggaa gctaaaacaa cgcattcgca gtttgcaagg tcatctttct
    12721 aatgtgcaag cagttggctt aatttctgcc ctaattcatc ccggtttgaa aaaccttatt
    12781 ctggcacatt taagcgaaat aaataatctt ccttccttag cagaacaaac aatgcgggaa
    12841 tatctgaata gtatccgcag cgatataaaa ctgtttgtgg cagaacaaaa tagacctact
    12901 cccccaatag aaatttaaag tatcacacct ctttgagtta aaatcggctt ttctcaatcc
    12961 ttttttctcg tttcattatt gtaaatatct ttcaaaccct tttcactttt acggtcgtct
    13021 tctgccgatt ttgactcaaa gttgtgggaa agttgtggtt gtcttctgcc gattttgact
    13081 caaagctgtg gtaaactcgt tttctgaatc ggcttttctc aatccttttt tctcgtttca
    13141 ttattgtgaa tatctttcaa ccccttttct attttacggt tgccttctac cgattttgac
    13201 tcaaacccgc gttaaaattc cgtaggaatg acagaaaaat aacgacgggt tttaacccgg
    13261 cgaaaatata tctctatgaa aaagaaagag ttccgtagga acgacagata ctaacgacgg
    13321 gttttcaacc cggcgaaaag aaagcaggat agtcattatc tctttttccg acaaagagtt
    13381 ccgtaggaac gacagatact aacgacgggt tttgaacccg gcgaaaagga agcataagtt
    13441 atttttatta ttccctggat tccggcttta gcaggaacga cattagaaca atagaattta
    13501 tatcctctaa taaaattacg cgttttatgc ctgataatta ttaccattta gggataaatt
    13561 gcctttctct atattcttca tctgccctat tcttgggatt ttttctcttt attatttaaa
    13621 tattctcagg gttaaaatcc gagctttttg ttttgctatt tccccgggat tttctcaacc
    13681 attgatgttt tataaataaa aatatgatat aattcctatt tctgaaaaat caggataggt
    13741 agcattcttg cctgcttgaa tattttatga caaagtaaca accaagtaac aaccccgtaa
    13801 cgagacaacg gaagtgttac ggaagtgtta cggagtcatt acttaatcat ctatataaca
    13861 agcaattaca taaatatagt tcagggtgga caaaatattc agttatctat ttaggtttta
    13921 taaggttaag attaatattt aggagttata atgattttta aaatggaagt ttgacctcct
    13981 ggtcaactac cttttaacct ctatttcgtt accgaaaata tcacaatttt tcaatcattg
    14041 tgtcgccctt aggggctttt tttagtggga aagggagaaa gaaaaaaaat cggggctttc
    14101 attttaacga ggggtttacg ccaccatcgc tatcattgtg tcgccctttg tgctttgaag
    14161 gaatctcaat cctctcaacc ttctcaacct tctcaaccct ctcaaccctt ttttccgagg
    14221 ggcatacgcc actattgcta tgtttgtttc gcattgtaga attgttacaa tttaaatgta
    14281 tttttccttg tgtagtgaac aaaaatgaac atatattttt gggtattaag gtgttaccac
    14341 tccgcatttt atattacatc agggtattgg ggtcaatatc aatctgcttt cggatattgg
    14401 aagggcaatt ataaagggaa ttaaataatc tgatagcgtt ctgcagaatt tttacggttt
    14461 gggctttaaa aataagatga tagcggtaat aagaactgat tttgctgaaa ggagccactg
    14521 aaggaccaag taataatagt tgtggcatag gaaattgttt gcgtaaaaaa taaaggactt
    14581 ctctcatatt gctcatttct tttttcaggg tttccaaatc ggtgctttga aacaaaattc
    14641 tggctaaacg gtaatagggc gggtaataga gcttttttcg gtaaccaagt tcttctgtgg
    14701 caaaacgaat atagttttgt tgactggctg attcaatagc gtaatgctgg ggattatatg
    14761 tttgaataat aacttctccc gctttatctc tgcgtcctga gcgtcctgca acctgagtaa
    14821 gcaattgaaa agttctttct gcggctctaa aatcgggcaa atttaaggaa atatcagcca
    14881 tcacaattcc taccaaggtt acaagaggaa aatccagacc tttggaaatc atctgcgttc
    14941 ccagtaatat atccacttcc ttatttttca tacggttata cattgattta taggtatccc
    15001 ttttgcgagc tgaatccgaa tccattctta agatttttgc ctctggaaac aggattttta
    15061 agttctgttc aattttttga gttcccggag ctccataggc aaaagaataa gagccacaat
    15121 aaggacattt acgcggactg ggaattgtgt ttccgcaata gtgacattgc atttcttcgc
    15181 ggtcacgatg ataactcata ctgatttcac aattcttgca agtaattagt tttccgcatt
    15241 tcaggcattg cataaaagat gaataacccc tgcgattttg aaaaaggata acctgctctt
    15301 ttctttggag gcgtttatct attgcttcca aaagaggagc ggaaatcaaa ctttgttcat
    15361 attcatctct taaatcaaca acttgcacat ctggcagaca ataatccaaa ggacggcttt
    15421 ctattttttg caatttatat tttccgttta gagcattata ccaggattcc aaagctggtg
    15481 tagcactacc taaaattact tgtgcatttt gtatttgagc tcttacgata gctaaatccc
    15541 tgccttgata acgaggtgcg ctttcctgtt tgtaagttcc ctcatgttct tcatctacaa
    15601 taattaagcc caaatcgggt aaaggagcaa aaacggcact gcgcgctccg ataacaattc
    15661 ttttttctcc gctttttatt ttctgccact ggattaaacg gtctttttca gtcagctgac
    15721 tatgctgaat tgccagaatt tgtccaaaat tactctgaaa acgatcaacc atttgcggag
    15781 tgagagcaat ttccggaatc aggaaaataa cggttttgtt tacggataga taatgcctga
    15841 taagctcaat atagacctct gttttgccac ttccggtaat gccatacagc aagtttacat
    15901 taaaactgcc aaaaccctct gtaatatctt taattgcttc aacttgggca tggttgaggg
    15961 caatttgttt taaaggcgag gaattttcaa aagaaatgga ctcgggatca aaacggcgtg
    16021 gttctatttt aataaaccct tttttaacta atgccttaat tgcactgtaa gagattgtgg
    16081 aacttatatt ggacattgga aaagagggtt cttcctcttt aattttttcc catgcctctc
    16141 tttgttttaa gggtaaagta tcttcctcca aagttggttg caaacggatt ataaaattta
    16201 ctgttcgggg cttatcttta tgatttaatc ttctctctat tttcagcaat cccagttctt
    16261 caccctcttc tattctttta tagagaggat atgaagtcag ctctttccgt aatgttttaa
    16321 gaacaggatt ttcactttta tctattgcct tttttaaggg tataaaaggt tccggaacat
    16381 tctctgaaag ccaaataaca tttgcctcca tttcaggcag caaacgcgca ggcaacatcg
    16441 cgaaaagtgc cttgccaact gaacaatgat agtaatcagc catccaataa cccaatttca
    16501 tcaattccgg tgaaagcacg ggaactgtat ccaaaacctc tataactgcc ttatatctaa
    16561 tatccttatc ctcttcggga cgggtttttg ctccgcaaat tcctgtccat aaacgcccgg
    16621 aaagatttac tattactctt gctccttcca caatttctgc ggatgaacaa taaatcagtt
    16681 ccttgggtat ttccaaaggc aaatgaatta cataataatg cataagacga aacttttgtt
    16741 tggctatagc ttgtcaaccc caaaaaaggt ctggaggtct tggagtaaaa tctaaagatt
    16801 gacaataaaa caaagttcca ataaattgaa acagtgaaga ggataatatg aggaaaaagg
    16861 ttttaacggc atttatgttg ggttttatgc ttatttcaac cttaatggga gaaaatatat
    16921 cttctcctgt tctctcccag acaaaatccg atactttata tttacaggat ttttccctgc
    16981 tttccgaaga tgatttcagg gagctggaag atttattgaa agaacaacaa ttaaaccctc
    17041 aagacctgaa ttttgagaag gattgggacc tctctacctg gggtaaaagc aaaagttttt
    17101 tacgctcttt gcaagagccc tattacggtt tgggtttaat gcaggaaatt agagaagcag
    17161 ctaataaagc taatacggat gagggctttg cttttctggt tgatttactg tataaaaatg
    17221 cgactgatgg tgcaggttca tttcccctct atctgaatta caaggactta tatcaacagg
    17281 aattcaataa aaaagtaaaa cagcccaaag atctgttccg cttttatgaa aaagcattaa
    17341 aagatattat tcccgttttg gcgaatgctt attcgcatta taaccttgat ttatggaaca
    17401 aatatgtttt ctatcttttc gttgccttaa gggaaaatga ggatagcgaa gcttataatg
    17461 cctattttaa taagcataat atacctcttt gggaagaaat agaagatcag gaaatcaagg
    17521 aactaatgga gctttttcag gaagctgatg ttttaaaagc cgctatgcag tttctggctc
    17581 tatgcgatgt aataaaaaat gaagcaggta aattaaacta cactaataaa aagcccataa
    17641 ttcaagaatg cgaattcgga actatggtta tcggaacgga aaaagatgat gtttataacg
    17701 ctaaaatgtt caaaaagccg cttattttgc ttattgaacc cgcaggaaat gaaaaatata
    17761 cgatgacttt ggcaacgggg gctaaaaatt atgcttatct gctgattgat tataaaggaa
    17821 atgacagtta tgtgaaccct gatatcggaa gtttgttttt tgctctttcg ggcttaggaa
    17881 tttcttatga tttggaagga aatgatattt acagaacagg tgatttttct tttgcgggaa
    17941 tgatgggaat tcaactccat cgtgattttc aaggtacaga tatttatgaa agcggttttt
    18001 tcagccaagg tgccgctata cgcggtcttt ccatccttca agatatgcag ggaaatgata
    18061 tttacagcgc tacttgtctg gctcagggtt ttggttctat ccgttctgca ggagttttat
    18121 tggattttga tggagcggat aattatcttt tagggggaaa atactttcat tcacccttga
    18181 tgccaaacga ttatcttact ctgggacaag gtgttggttt tggtttgcgt cccgattttg
    18241 ccggtggatt agggcttctt tttgataaaa atggcaacga tcgttatttg ggaggtgttt
    18301 atgcacaagg agttggctat tggtatgctt tgggaatgtt gattgatgaa gctggaaatg
    18361 atgtttacaa tgctgtatat tatcctcaag ggtctggaat tcatcttgct tctggttttc
    18421 tttttgatgg cagtggagat gatgcatatt acagcagaca cggtcctggt caaggtgcag
    18481 gtcacgattg gggtttggga atttttattg atgctaaagg gaatgatgcc tattccattg
    18541 aaggaggaaa cggtttgggt ttaagtaatt cagtgggaat ttttatagat aaaggcggaa
    18601 atgatcgtta tgaacgcaat aatcctcaaa attatggtaa tgctgtctat agcagagcaa
    18661 ccggtggtat aggtcttttt ttggataaag atggcaaaga tagctatccg gatactttga
    18721 tggctgaggg aacaacctgg aaaaaaggca cttatggcat tggcagagat ttggattctc
    18781 gaaatttgat gtcctcatca acaaccgatt cagaaacaaa agaaccggca gaaattccaa
    18841 tgttgcctcc tccttctccc gatgagccca tagcagatat tttttctgct gctgctgaat
    18901 gggaagttgg caacgctatt gaaagagtga aaaaagcccg ggaaattatg cttgccagag
    18961 ctgatgaggc aattgattat gtgataaaga ataagcttga taccaagtcc ggattggaat
    19021 atagagcttt agaggttctg gtgaaagaaa atgagcagtt taaacaactt ctttttgatt
    19081 ataccgatga tatagatagt ttgaaagcta aaaatgccct gtctctaatt gccggagttg
    19141 gagatagcac tttaattgaa cccttgcgca aacatttaaa caacggaaaa tatatcacca
    19201 cctgtctttc gcttttgggc agcataaaaa gtgcggaaag cgttgatttg ctattccaat
    19261 atgcttttca tccttcagag cgttatcgct atattgtagc ccgttcctta aaacaaattg
    19321 gaacgcctga agcgcataac tgtctgaagc aaatggcaaa tgacaattcc tttctggtga
    19381 aaaccttagt tcgtaaatgg gaagaggaac aacaatgacg gaagaaaaac aaatttttga
    19441 ggactatctg cgctttctcg gtttggagga tatagaccat ttaatggctc gttttgaaca
    19501 ttacctgcat ttactttata atgccaaccg caaagtaaat ctcgtttccc gaaacattcc
    19561 cctggaaaga tattgggttc agcatttcct tgacagcata gttgctttgg aatgtttgga
    19621 ttttacagat aaaaccgttt tggattttgg ttcgggaggt ggtttaccag gaattcctct
    19681 gaaacttgct gttccggatt ttaatttgac gcttttggat tcggtgcaga aaaaagccag
    19741 ggttttgcgt gaatttgtgg atgccttatc tctttctgct acaaatgtgg aagccagtcg
    19801 tttagaggac tatgcttatg tggcaaagcg tcccagctac gattttattg tttgtagagc
    19861 cgttgctttg gaagaaagat acttttctcc tttgcgaaga ttgctgaaac caagcggaat
    19921 ggttgttttt tacaaagcac agaaattaga cgatgtgcaa gttctaaaat atgagattct
    19981 tttggataaa gattatcctg ccttaggtca acgcaaatta attggtatca aacagcgtga
    20041 ccttctcttt cattaagttc ttaaaaatta aaggatatat atgcccaagg taataactgt
    20101 tgtaaaccag aaaggtggtg taggaaaaac aactaccgcc gtaaatcttt ctgccggttt
    20161 ggcagttctg gaaaaaagga ctctgctgat tgactttgat cctcaaggta atgctaccag
    20221 cggagtagga attgataaag ataaagtgga actccaaatt tatgatgctc ttataggcag
    20281 agctcctata gaaaaaacta ttctctccac agccaccaaa aacctttttt gcattccggg
    20341 aaatatcaat ttaaccggag cggaaattga actggtgcat gaatttgccc gcgaacagaa
    20401 attaaaagaa gcactccaac caattttaaa tacttttgat tacattataa ttgattgtcc
    20461 tccttcactt ggtttattaa cggtgaatgc aatgactgcg gcaatggagg tcttgatacc
    20521 tattcaatgc gaatattatg ctctggaagg agttagtcag ttgctaacaa ccattcgctt
    20581 gattcaaaag aacttaaacc ccggtttaaa cattctgggt gtgctgttaa cgatgtttga
    20641 taaaagggta aacctctctt tgcaggttgc taaagaggtt catcgctatt ttaaggaaaa
    20701 ggtcttccgc actattattc cccggaatat taaattaact gaagctcctg gttttggtaa
    20761 acctatattt ctctatgaca taaggtctcc aggcgcaatg agctacctaa atttggcaaa
    20821 tgaagttatc aatagaagcg aataaggtaa taaaaaatga atgaacattt gggaagagga
    20881 cttttcgctc tgattccgga taacgataga gaacaaactc ctcaattggg aattactact
    20941 ttgcctattg caagtattaa gcccaatcgc tatcaacccc gaaaaacatt tgacccggaa
    21001 aaaatggcgg aattaactga atccatcaaa gaaaacggta ttattcaacc cctgattgtt
    21061 actaaaacaa gctcttcgga atatgaactt gtggcaggtg aaagacgact ggaagcagca
    21121 aaacaggcag gactggaaaa agtgcctgtt gttatccgca gcgtttctaa aaaagaacag
    21181 ctccagctcg ctctaataga aaacattcaa cgggaggact tgaatcctat tgaagaagca
    21241 attgcttata atactctggt tgaggatttt ggtttaacgc ataaccaaat ttccgaaatt
    21301 gtaggtaagg acagagctac agtcactaat tccctgcgtt tgctaaaact tcctgaagaa
    21361 gtaaaacaaa tgcttgtttc cggagaattg aattccggat tggctcgcac tgttttatct
    21421 gtgcctccgg aactgcaaaa tagctttgcg gaatacataa taaagtataa tttaacggtg
    21481 cgtcaggcag aagaaaaagc gaaaaccttt tctctcgcta ataaggaaga accggaaaag
    21541 gcaaaaacaa atgcccgcac caaagaactg gaaagggaac taagcaattt gttttgcctg
    21601 aaagtgaaag tgcaggaaaa taatggcaag ggcaaaatta ctttggtgta taaatctcca
    21661 gaagaactgg aggcatttag aacaatccta aataaaataa ggcaggaaaa atgaacagag
    21721 ttattgt
//
LOCUS       DAOPFL010000023        20472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2806_length_20472_cov_123.438556, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000023 DAOPFL010000000
VERSION     DAOPFL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 20472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20472
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2806_length_20472_cov_123.438556"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..3455
                     /locus_tag="PLG69_03055"
     CDS             15..3455
                     /locus_tag="PLG69_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPI42519.1"
                     /translation="MKKTFLLFILALCSIALFAQNEDWHWAKKAGGTGGDYCRSIAVD
                     DNGNSYVTGVFEGSATFGTTTLTSSGQYDIFVAKMDINGNWLWAKKAGGTSYDYGLGI
                     TVDANGNSYVTGYFLESATFGATTLTSSGNEDIFIAKLDINGNWLWAKKAGGTSYDYG
                     CCIAVDDNGNSYITGFFCSSSCSFGNTTLTSSGSDDIFVAKLDSNGNWLWAKKAGGQS
                     NDGGRSITVDDNGNSYVTGDFSGSATFGTTTLTSSGYYDYDIFVAKLDINGNWLWAKK
                     AGGQSNDGGRSITVDDNGNSYITGCFWGSATFGATTLTSSGDLDIFVAKLDINGNWLW
                     AKQAGGTDFDYGYGIAVDDNGNSYVTGYFRSSATFGTTTLTSSGYEDIFVAKLDSSGN
                     WLWAKKAGGTSYDKGYSIAVDVNGNSYVIGYFSGSATFGTTTLTSSGDTDIFISKIGI
                     PVYQVLAPNGGEQWQTGSTKTVYWNFNIGNYVNIQLSLDNGVNWIMLNSSPIGALLGR
                     FSFTVPYVSSNNCLIKVVSTENSDYFDISDATFTIINSVPYSLLLTAPNYSKLQAGKN
                     YSINWTAAGITRVNLAYSCDAGVTWNSIVTALPANQETYEWTVPDISASNCYLKVSDS
                     AQPAIYDWSDQPFSICNLNLLSPNGGEVYAINTNLYISWNSELIETVKIEFSSDNGNS
                     WLTIANNVSAAAGSYSWTTPDINSSQCLIKISDATDNTFWDISDNPFTIRPQIIVIAP
                     NGNEYIAVYSIYSILWSNTEEVSFVLIDYSIDGGVNWLPVQTSPYPASTGRYDWLVPN
                     NPSTNCLIKVRNSANSAIFDVSDGVFTITTGLYPPTVNFTADVTSGLQPLEVHFTDQS
                     TAGTGSIIFWNWEFGNGDNSNEQHPVYVYQDPGVYSVTLTVTNSVDSTATLTRDNYIT
                     VIQRVPEIEVNPPTMLNFGSVYLGSSSTLYSVVISNTGTAELLIDNLSLAESNSFFSF
                     VNTVLQLTIPAGEETEIALLFTPQEAGTVSDTLYIHNNSVNSPLYTLELRGTGEYVPP
                     KPPNNLVCVMDGYNAVLTWDAVTETIFDTPIVPDYYLVFYNGSANPEGEFYFHGATPD
                     LTYTHSLVGLHSPYMFYRIVAYKFYGREKVSIEDLGLTRGMTETEVRNILQAVETRIK
                     "
     gene            complement(4512..5909)
                     /locus_tag="PLG69_03060"
     CDS             complement(4512..5909)
                     /locus_tag="PLG69_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HPI42520.1"
                     /translation="MQYSTMQSISEQDILPEPIIGGNKQESKFNVDLLPDFLKEYLRI
                     ASQVCDAVPEALITALLPIIAVNIGNKVYMTQGAKKTYPNIYATLVGPSGCRKSSSMA
                     TAKLTMEKYNEELDKMNSAEREKNTLILNTVTQVELGHLLELNPARLLMFDEIGEFLK
                     SCNMKHNYGYKENITKIFDTDNYSYMTTLNSTFIKNPALSILGCSTEGWIYRDFQNTT
                     DQNSGFLQRFIVCVINMDKINLVSENNENEEAMDELRKYNEKYEVFRSIPKQYKIKLS
                     EEANTYWKNYSKVIEQDIGNEYYQKDMGYAIRIFNDYFLKLCMIFTLMENYQELKTAI
                     DNGTCDDFFANLYISEQTAINVFYLCRYYLQQTQTFTTIIEIGDKFKNEKRIVEYLSN
                     CPQNKASKTNIMNHTHLNSNELSICQKNLEEQEAIQIYCDDSTKSTNAKKPTTFFKLT
                     NRAFNLYRIKKKNHN"
     gene            complement(5899..6930)
                     /locus_tag="PLG69_03065"
     CDS             complement(5899..6930)
                     /locus_tag="PLG69_03065"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HPI42521.1"
                     /translation="MLKGIIKTKVKVPNFSPNYDAEQPENKKKGHQAIVFVDLNNAWL
                     YYEQLGYKLVTFTLEEKKGKKVVSNMPQWKNSSLSVESIKQEHNAIAIITGEASQLMV
                     IDIDKRDADIYALLAEYGLEIDNYCYALTPSGGMHIYLNLSHSELWKKRYGRKTLTTT
                     NKNIGIDIRAEGGLIFAPPSIVTNGGFYEWVNMPVNKEDTDYDPNKLIPIMDAIFGYN
                     NNPTPLAPIQKTITKSYFPFTYYPNIQDNYDQAAQLIRKLNGTIINYNDWIRMGIALK
                     NEFGKRGLSLWLLFADNSAYQDTEEYLIKKWNSFPITDDVHFGTFIYLTREYLRYENT
                     YKGGKIYAV"
     gene            complement(7280..7792)
                     /locus_tag="PLG69_03070"
     CDS             complement(7280..7792)
                     /locus_tag="PLG69_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42522.1"
                     /translation="MNKPVEPIQTMLQTYPNFKGSKVIDLLYAHPDTPIPAADMELAL
                     NLQIPPDFINRNRYRFAPIRMTDEQTLRCVDKRLNRLIELKAFNATTAYDDEIQALIR
                     YRKETTLPTGKIKCFNDDDSKAYDRLRKDIDTLLKQAEKDGYSEAVAIVKRCLHRGLN
                     FFWDSRCSET"
     gene            7905..8036
                     /locus_tag="PLG69_03075"
     CDS             7905..8036
                     /locus_tag="PLG69_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42523.1"
                     /translation="MKRGERRVSQRGKERKREFYKEEKRERENFTKRKREKEKIKSA"
     gene            8110..8547
                     /locus_tag="PLG69_03080"
     CDS             8110..8547
                     /locus_tag="PLG69_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42524.1"
                     /translation="MIVESKFELLFCGNCDGMVYYYNKRLGKMIARKWVKPKTTAANR
                     RLGAITRNLKSLKPSEGFIKDLRVYIALYDYSPGERHYMSWQNVYLTLMYALAKQNPA
                     VDLLTITREQITSDNLPCKSVKDAIEAGLLREVNGYENLTQEI"
     gene            8578..9030
                     /locus_tag="PLG69_03085"
     CDS             8578..9030
                     /locus_tag="PLG69_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42525.1"
                     /translation="MKVKFKYGIRTYSGTLDELTFGSYFKDRVCIARRYVVPRLTDQN
                     DLIGSKMKNLAVIYGDVSESYKVELKQYAAMHSILTPKGKLPATAYALWVKMMFQFAS
                     ADAEHIDLSTLTYSDLQTVGDDILSISSAVSNGFMDNVPGADKLTANM"
     gene            9326..10516
                     /gene="pgsB"
                     /locus_tag="PLG69_03090"
     CDS             9326..10516
                     /gene="pgsB"
                     /locus_tag="PLG69_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HPI42526.1"
                     /translation="MTVLIIVTLILITHWIIEYQRHTKNVLSIPIRIHINGTRGKSSV
                     TRLIAAGLREGGKVTVAKTTGTLPRVILPDGREAAILRLMGANIIEQKYIFRHAVKHK
                     PDAIVIECMAVNPVLQWITERKFIRSTISVITNCREDHLDLMGSTVQSVTMCISNTIP
                     KKGICYTAEEEQYPLLEKVAKSRQCQIHKIRPVDVTEEELNKFRYIEHSENVQLALAV
                     CAAAGVPRDVALRGMQKATPDPGALKKYPIKDRGKTIIFYNVFAANDPQSTVEIIQMV
                     TGNLQNIEKIIILNSRADRLFRSHQLVDAIKPLDFSYLLLTGEIPDKIENYAIQAGIP
                     KEKIFPLGEPLPEVIYQKVWELTKDESHILGIGNIAGTIKYGAQIVAHFRHKMKECNE
                     RSRM"
     gene            10513..10968
                     /gene="pgsC"
                     /locus_tag="PLG69_03095"
     CDS             10513..10968
                     /gene="pgsC"
                     /locus_tag="PLG69_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HPI42527.1"
                     /translation="MIDTIVQAAVGMGVIISLMFSELLGASAGGIVVPGYIALYLDRP
                     LQILGTLVISLLTWAIIRIIGNFTLLFGKRRMVLSILIGFILGWLSRILVVQDITIHQ
                     LQMQSIGYIIPGLIANWFERQGFWKTVSSMGIAAIVVRLALMVIFNGEL"
     gene            10971..12095
                     /gene="pgsW"
                     /locus_tag="PLG69_03100"
     CDS             10971..12095
                     /gene="pgsW"
                     /locus_tag="PLG69_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPI42528.1"
                     /translation="MMYRPSLKSNRSLIILLLLAIVLFYIAQTSYVNVKTDNYEIKIA
                     AAQLMKSAIDSLSAEINKRGIEIDPIDDPLQTGLIGMRLSSITTDRGLLSEKRAAINP
                     NLAAVFVEELSKMKLMPGDYIAVGITGSNPAVNIALYSAIQVMELNPKIIVALSSASY
                     GANRPELTWLDMESILKEKGLFDFGSSYASFGGKDDLAIGLSDNGIKALREAMQRNNV
                     PLLIGNDLEDNIAIRMAAYEELLPEGKRYKAFVNIGAGLANVGSEPNANLIPEGINRR
                     LAEHNYEKEGVIIKMAKNNVPVLHFRRILRWAKRYNLPTSFDTMPKVGEGKMFGSRIH
                     NQTVNIICLTILLIAIVIVIIFDRHDRRFMANIVDLDEEL"
     gene            12108..12929
                     /locus_tag="PLG69_03105"
     CDS             12108..12929
                     /locus_tag="PLG69_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42529.1"
                     /translation="MHKHFISVLLIILGINLLLAQTVNRYKVLPFDNPGTKLLLKTEA
                     GNYYYYRGLPERPLILNTTGVEKIELRSFSKEAVRKPEIVIVINKQRKTYPLSFKEKK
                     GNYYLYQPLMIDIPENTKEIQIICYDRSIYMRAFSVLPPKPPKAAKQPNIAIKAHSGT
                     VSLQHNGSNSDYYTFMPNQPFKFTLNNGRNAYVYVRPRLLDCSLPKIGLYANGELVEV
                     IDFTLKRTTKYSCQGISSLGIAKKIVLPANEKSTDYELRALSDHLFLAKPILMKK"
     gene            12939..14090
                     /locus_tag="PLG69_03110"
     CDS             12939..14090
                     /locus_tag="PLG69_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42530.1"
                     /translation="MADTNPKIFPQEAKPIAELKEKETIASLDSLSPKQTKENKTVKK
                     SKIRRKNSIRPIILLLTFFLFFGFSQVEAQINGSVKIGAVYSDNVFHLSEYDIDRFDD
                     HHPNLNFVDTIDDLTLNTRIELKYPLSYRWWKFTPSVSGNFSQNISNEDKYRTDWTLK
                     FKVDRYYWNCSVQYTSNPYIYFRHFVDTDGTGELEKYSYSRDTYRGDLAVKPLPKTTV
                     KANLRYELYRYNKYFTEADGKAITGEAGVSYKFPFFTVEGSYGYRDFSCDKRIANKDC
                     SYQSNIYKGALTLPKMPLSEKGKTLIQPSFALNYEQRFYQGSGSWYGGRADYTYSLMA
                     GMELFFSPKMNLSLDYSHYFRNVETDNETVQRLKEYSENRLSAVLSYKF"
     gene            14106..14438
                     /locus_tag="PLG69_03115"
     CDS             14106..14438
                     /locus_tag="PLG69_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42531.1"
                     /translation="MVFKKENDLRKIKEFLTLVENPKVELRHYKKPVLVWAVDEGTVF
                     YSFWEEEMLTRFGLENVDGWDFQEDLLFCPDWIGDTEEEDLEDFDLDDEDIEELSHFL
                     HRNMDDKD"
     gene            14441..15448
                     /locus_tag="PLG69_03120"
     CDS             14441..15448
                     /locus_tag="PLG69_03120"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPI42532.1"
                     /translation="MRIATLGGGGWGLALSSLLSENGHQILVWEYNPEYLILLKETHS
                     NPHLLPNITLPEEVCFTNDFQEIVAFSPEIIILATPSQFLRSTLQKTPVSLWNRPELL
                     AVVNVAKGIEENTLKTLDAVIKDELPFLEEHKICALSGPSHAEEVARKIPTTVVIAGS
                     DEDLLCYLQTVFSNSYFRVYRNLDLIGVEIGGAVKNIIAIAAGIIYGLDFGDNTIGAL
                     LTRGIVEIQRLGIALKALPETFLGLSGIGDLITTATSLHSRNRFVGVEIGKGRKLSDI
                     LAQMEMVAEGVTTTRSVYLLSRKLRIEMPIVEKVYQVLYEDKEPRKAILELMTRELKA
                     E"
     gene            16360..19308
                     /locus_tag="PLG69_03125"
     CDS             16360..19308
                     /locus_tag="PLG69_03125"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPI42533.1"
                     /translation="MLQKFLMSVVILMCVYSSLSALRYTTLEDFESGFVSLSSWSIEE
                     DLQPDAWSLDTSTPDSSSYCLKLTGNCYKLQQITPYAIDSTGVIQVQVKTSSNPRIQG
                     IGFTDGVHNILYSFAGSRILDIEVWIPVYQGAFSNGVWNTFQLPLALDWQAFWGYLPV
                     INGIIYINDLDGISSRSVWFDNIIDISSDLPVPPTVSISTSYPVKNTGVYFYSNVIDP
                     DSDTFTYEWSFGDSTFSNLPNPYHLYTIASAYPYTVTLKVTDDTGKVGFASTEVTLET
                     GASHLPVTLNFVGDIMLARRYENPGGIIPTLGVNAIFSPTKPYFGDAADISVANLEVV
                     LANVGVHHPTKSVYYRGNPANVSGLVYAGIDVVSTANNHTMDYGIEAYQQMQGLLNNS
                     GIKYSGCGANSYEAYLPTFYHCRGLNIAFLRSSDRTGQYNNAQPFLQAGYNKPGFAYM
                     TPYYVEQQLQAVEGIADLKIVEMHGGSEYSIAPGAGYDKEFNPFLEDTEDEDYFYRND
                     VPHQWDIEIRHSAIESGADLVIVHHPHIIQGLELYQNKLIAHSLGNFVFDLDYPETMP
                     TMILYADAYFDGFRNFRIKPFYIDQYIPKPATGQLAVYILDYIASRSMDFNTRVMVDK
                     ENLEARVLMEDENPPVTTNAFSSWQHLLFHSGGMSYTSPIKLPRRGSISNIINVEPPA
                     EYQFRLGQEHIWFGNFENEGSTLWIPPAYSTTDFIDGERSAKLTTTGAGSVTSTISQR
                     CKIYDNTKKYTLHGWLKTENVSLANISIRFYTSRTAAVASYTDYVTENIIGTNDWTYY
                     YKELNLPTDVFYYEIHLQMTGSTGMTAIALFDNVGLIEWTEWTATADLINIPWPNNYY
                     WIQARTADTPKSMSFSLVERSFGVQRANASNHFSKMKPELNISPNPFNPETIIYCVLP
                     VSGKTTIKIYNIKGQLVKELYKGELEAGIHRFNWDAKDIDNRSVGSGVYFIYLENGSS
                     KVIRKAVLMK"
     gene            19384..20052
                     /locus_tag="PLG69_03130"
     CDS             19384..20052
                     /locus_tag="PLG69_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42534.1"
                     /translation="MRKFLLTLVLLVLAFCLMAEEADTPKINFALLNPKEINLELRVY
                     APMQRVYITADFSVEKDSLPDAVYHSLFLTKDARIEYFLINDKFVPPVYSTKLVPEHF
                     NPVFPYPEMLNEDSNLNCFSFYLPNYEGKIKFTLRYSIPIPEWVKESDIRGYVTFSSA
                     QNWYPRNLTGPCKVYARLISSTYYTMELGTQCTIKEKDDIRITEGYFIDSPAEQSIFK
                     IIKS"
     gene            20048..20123
                     /locus_tag="PLG69_03135"
     tRNA            20048..20123
                     /locus_tag="PLG69_03135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20081..20083,aa:Thr,seq:cgt)
BASE COUNT         6360 a         3513 c         4165 g         6434 t
ORIGIN      
        1 tcatggagga tattatgaaa aagacatttc tactctttat cttggctctt tgctctatag
       61 cactctttgc ccaaaatgag gattggcact gggcaaagaa agccggcgga acaggtggtg
      121 attattgccg tagcattgct gttgatgaca atggaaatag ctatgttaca ggtgtttttg
      181 aaggtagtgc tactttcgga actactacat tgaccagcag tggacaatat gacatctttg
      241 ttgctaagat ggacattaac ggtaactggc tgtgggcaaa gaaagctggg ggaacaagtt
      301 atgattatgg tcttggcatt actgttgatg ccaatggtaa tagctatgtt accggttatt
      361 ttttagaaag tgcaaccttc ggcgctacta cattaaccag tagtggaaat gaagacatct
      421 ttattgccaa gctggacatt aacggtaact ggctctgggc aaagaaagcc gggggaacaa
      481 gttatgatta tggttgttgc attgctgttg atgacaatgg aaatagctat attacaggat
      541 ttttttgtag tagtagttgt tcctttggca atactacatt gaccagtagt ggtagtgatg
      601 acatctttgt tgctaagctg gatagtaacg ggaactggct ctgggcaaag aaagccggag
      661 gacaaagtaa tgatggtggc agaagtatta ctgttgatga caatggtaat agctatgtta
      721 caggtgattt ttctggtagt gctacattcg gcactactac attgaccagt agtggttatt
      781 atgattatga catctttgtt gccaagctgg acattaacgg gaactggctc tgggcaaaga
      841 aagccggagg acaaagtaat gatggtggca gaagtattac tgttgatgac aatggaaata
      901 gctatattac tggttgtttt tggggtagtg ctaccttcgg cgctactaca ttaaccagca
      961 gtggtgattt agacatcttt gttgccaagc tggacattaa cgggaactgg ctctgggcaa
     1021 agcaagccgg gggaacagat tttgattatg gctatggaat tgctgttgat gacaatggta
     1081 acagctatgt tacaggttat tttagaagta gtgctacttt tggcactact acattaacca
     1141 gtagtggata tgaagatatc tttgttgcta agctggatag cagcggtaac tggctctggg
     1201 caaagaaagc cggcggaaca agttatgata aaggttatag cattgctgtt gatgttaacg
     1261 gaaacagcta tgttataggt tatttttcag gaagtgcaac ctttggtact actaccttga
     1321 ccagtagtgg tgatactgac atttttatct ccaaaatagg tatacctgtt tatcaggtct
     1381 tagctccgaa tggaggtgaa caatggcaga caggaagcac aaagaccgtt tactggaatt
     1441 ttaatatagg caattatgta aatatccaat tatcattgga taatggagta aattggataa
     1501 tgctgaattc cagtccaatt ggagcactat taggcagatt ttccttcact gtcccttatg
     1561 taagctccaa taattgtcta attaaagtag tcagcacaga aaatagtgat tattttgata
     1621 tttcagatgc aacatttact attatcaatt ctgtgcccta ttctttattg ttaacggcgc
     1681 ctaattattc taagctgcag gcaggaaaga actattctat taattggaca gcagcgggaa
     1741 tcactagggt aaatttagct tattcctgtg atgccggtgt tacctggaat agcattgtca
     1801 ccgctcttcc tgccaatcag gaaacttatg aatggactgt tcctgatatt agtgcttcta
     1861 actgctattt aaaagtatct gattctgccc agcccgctat ctatgattgg agtgaccaac
     1921 catttagtat ttgtaattta aatttactca gtcctaatgg tggggaagta tatgcaataa
     1981 atactaactt atacataagc tggaattcag aacttattga aactgtaaaa attgaattca
     2041 gttcagataa cggtaattcc tggttaacga tagctaataa tgtatctgct gctgcaggta
     2101 gctatagttg gactacaccc gatattaatt ccagtcaatg tctgataaaa atctcggatg
     2161 ctaccgataa tacattttgg gatataagcg ataatccctt taccatccgt ccgcaaatta
     2221 ttgtaattgc ccctaatggg aatgaatata tagctgttta tagtatttat agtatacttt
     2281 ggtcaaatac tgaagaggtt tcctttgttt tgattgatta tagtattgat ggaggagtaa
     2341 actggctacc tgttcagacc agtccctatc ctgcttcaac agggagatat gattggttag
     2401 tgcccaataa tccatcaact aattgtctga taaaagttcg caattctgcc aatagcgcca
     2461 tttttgatgt atcggatggg gtttttacta tcactacggg attgtatcca cctacggtga
     2521 attttacagc tgatgtcact tccgggttac agcccctgga agttcatttt acagaccagt
     2581 ccactgctgg aacgggaagt attatctttt ggaattggga atttggcaac ggcgataatt
     2641 ccaatgagca gcatccggtt tatgtatatc aagacccggg tgtgtattcg gttacgctaa
     2701 ctgttaccaa ttctgtagat tctactgcaa cccttacaag agataattat ataactgtta
     2761 ttcaacgcgt tcccgagata gaggttaatc cgccaacaat gctgaatttc ggtagtgtgt
     2821 atctgggcag tagttcaact ctctattccg ttgtgataag caataccggt acagcagagt
     2881 tgcttattga taaccttagt ttggcagaat ccaattcttt cttcagtttt gtgaacacag
     2941 tgctgcagct gactataccc gcgggagaag agaccgaaat agctttgctt tttactccgc
     3001 aggaagcagg aacagttagt gatacccttt atattcacaa taattcagta aactctccgc
     3061 tttatacctt agagctgaga ggcacgggtg aatatgttcc cccgaaaccg cccaataacc
     3121 ttgtctgcgt aatggatggc tacaatgctg tgctcacctg ggatgctgta acagagacca
     3181 tttttgatac ccctattgtt ccagattatt accttgtgtt ttataatggc tcagctaatc
     3241 ccgaagggga gttttatttt catggtgcta caccggattt aacttatacg cactcattag
     3301 tgggtttgca ttctccttat atgttttatc ggattgttgc ctataagttc tatggcagag
     3361 agaaagtcag cattgaagat ttaggcttga ccagaggaat gaccgaaacc gaggttagaa
     3421 atatattaca agcagtagaa acaaggatta aatagctctg ggtttagttg ggtagacaca
     3481 agtcaggagt cgtcactgcc gaaataactt ttacgctatg aactaattcg tttggcagtg
     3541 aggactcatg acgactatct ctcacagatt acacagataa cacagatatg attttttgat
     3601 tggtctacag tgccgtcatt agtcatatgt gccacaaaag ttatctccca ggtgaaagtt
     3661 gtaaaggcac aaatgactct tgacttacat tccgtctacc ctgcttccct actggagggt
     3721 tgtctgactg taaacagtgt attgaagagt tctcaaactt tgttttagtg catataaaca
     3781 gacatatttg tggcacttaa gagggcagta gggaaggagg ggatattggc aaaaaagaag
     3841 gataataccg acaaaaatta ccaaggggtt gttactttat aacctgcaaa aaaagcgctg
     3901 gcagagagca aaggggtgat aaattcacaa ataggggtaa agttataacc tacacaaagg
     3961 gtataagtta gacataaaat cagaatattt aaacatctgt cggtgattta ataacctata
     4021 tatttatcaa tagtttagtt agcaaaaatg gaataaataa gcaacttaga gtatagacct
     4081 atagacaatc tttactgctt acatagaaaa gattttaatg aagtatacga caactattaa
     4141 tataaaagcc aataatggat tattaaaggt tatatgacat atatgttaat ttaaatacta
     4201 agttttatct catcagaata tgacgacatt tatattccag agctcttaaa aaatctatag
     4261 aaaagaacga ataagtcaac tatttatctt cagtttctaa acatagatta tgcaacagtt
     4321 attatccgtt aattgggcag tagggttatg tctaataatt gcttatttaa ttatatttta
     4381 atagataact atatagatgt caataagtta ataaatcaca ttaaatagca ttaaatcagc
     4441 aagaggggta gggagtaaat ataaaaaccc cccttcatac ggaaaggggg gtttattata
     4501 tcttcagcta attagttatg attcttcttt tttatacgat ataaattaaa ggccctatta
     4561 gtaagcttaa aaaaagtagt tggctttttg gcattagttg atttagtgga atcatcacaa
     4621 tatatttgaa tagcttcttg ttcctccaga ttcttttggc aaatggaaag ctcattggaa
     4681 ttaagatgag tatgattcat aatattggtt ttgcttgcct tattttgggg acaattagat
     4741 aagtattcta caatgcgctt ttcgttcttg aatttatctc caatttcaat aatagtagta
     4801 aaggtttggg tttgttgtaa atagtatcgg caaaggtaaa atacatttat tgctgtttgt
     4861 tcagaaatat acaagtttgc aaaaaagtca tcacaagttc cgttatcaat agcggtttta
     4921 agttcctgat aattttccat aagagtaaaa atcatacata atttgagaaa ataatcatta
     4981 aaaatgcgga tagcataacc catatccttt tgataatatt cattgccaat atcttgctct
     5041 attaccttag aatagttttt ccaatatgta ttagcttctt cgcttaattt tattttatac
     5101 tgttttggaa tactgcggaa aacttcgtat ttttcgttat attttcgtaa ctcatccatt
     5161 gcttcttcgt tttcgttatt ttccgataca agattaattt tatccatatt aataacacag
     5221 acgataaatc tttgtaaaaa accggaattt tggtcggtag tattttggaa atccctatat
     5281 atccaacctt ccgtagaaca acccaatata gacagagccg gattttttat aaaagtacta
     5341 tttagagttg tcatataact gtaattatcg gtatcaaata tttttgtgat attttcttta
     5401 tagccataat tatgtttcat attacaagat tttagaaatt cacctatttc atcaaacata
     5461 agaaggcggg ctggatttag ttctaataaa tgtccgagtt ccacctgtgt aactgtattt
     5521 aaaataaggg tattcttctc tcgctctgct gaattcatct tgtccaattc ttcattatat
     5581 ttctccattg ttaattttgc tgttgccata gatgaacttt ttctgcatcc acttggtccc
     5641 actaacgtag catagatatt aggatatgtc ttttttgcgc cttgagtcat atatacttta
     5701 tttccgatat ttacggctat aattggcagc aaggcagtga taagtgcctc aggaactgcg
     5761 tcacatacct gtgatgcaat cctcaggtat tccttcaaaa aatcaggtag taaatctaca
     5821 ttaaatttgc tttcctgttt gttcccgccg ataatcggtt cgggtaaaat gtcttgttcg
     5881 gatatagatt gcattgtact atactgcata tatttttcct cctttataag tattttcata
     5941 ccgcagatat tctctggtta gatagatgaa agttccgaaa tgaacatcat cggttatagg
     6001 aaaggaattc cactttttta tcagatactc ttctgtatcc tgataagcgg aattatcggc
     6061 aaacagcagc caaagagata aacccctctt cccgaattca ttcttcagcg ctattcccat
     6121 ccttatccaa tcattataat taataatagt accgttcagt tttctaatta actgagcggc
     6181 ttggtcgtaa ttatcctgaa tattaggata ataagtaaaa gggaaataag attttgttat
     6241 tgttttttgg ataggtgcca aaggtgtagg attattgtta tatccaaata tagcatccat
     6301 aatagggata agcttattcg gatcataatc cgtatcctct ttgtttacag gcatatttac
     6361 ccactcataa aaacctccat tcgttacgat ggacggggga gcaaaaatca gcccaccctc
     6421 agcccggata tcaataccta tattcttgtt tgtagtagta agcgttttac gcccataacg
     6481 ctttttccac aattccgagt gcgataaatt aagataaata tgcattcccc cggagggtgt
     6541 aagggcataa caataattgt caatttccag accgtattct gcgagtaaag cataaatatc
     6601 tgcatctctt ttatcaatat caatcaccat cagctgagat gcctcaccag taatgatggc
     6661 tatcgcatta tgttcctgtt taattgactc taccgataaa gaactatttt tccactgagg
     6721 catattactg actactttct tacccttttt ctcttccaaa gtaaatgtaa ccagtttata
     6781 acccagttgt tcgtaataaa gccaagcatt attcaaatcc acgaaaacaa ttgcttggtg
     6841 tcctttcttc ttgttttcgg gctgttctgc atcatagtta ggcgaaaaat taggtacttt
     6901 cacttttgtt ttaataatcc cttttaacat ttttaatcct caattaatta tattttattt
     6961 tttctcttct ttttttcttc ttgtgaaaat agcttttttt cataataata cttatcttat
     7021 ttaaggtagt acctccttat taaatatttt ttagaattta cattattagt tcataatcct
     7081 tatcctcatt tttaatgatt aactccatcc atattattat tgccataaat actattgtta
     7141 ttaatgtgtc aagctgattc ttaatatttt atcgccattt tattatctat gtttagattg
     7201 gttcattata agctatattg agaaaataag cgataatgtt ctatcatctt ctctataaat
     7261 aagttatgat tatcattctt tatgtctctg aacatctgct atcccaaaaa aaatttagtc
     7321 ccctgtgaag acagcgctta acaatagcca ctgcttcgct gtagccatcc ttttccgctt
     7381 gtttcagcag ggtgtctata tctttgcgca ggcggtcgta tgccttggaa tcgtcatcat
     7441 taaagcattt tattttacct gtaggcaaag tggtttcctt gcgataccga atgagggctt
     7501 gaatttcgtc gtcataagcg gttgtagcat tgaatgcctt aagttcaata agacggttca
     7561 ggcgtttatc tacacagcgc aaggtctgtt catcagtcat acgaataggg gcaaaacggt
     7621 aacggttgcg gtttatgaaa tcaggaggaa tttgtaagtt aagagccaat tccatatccg
     7681 cagccggaat cggggtatca ggatgggcat aaagcaggtc tatcactttg ctacccttaa
     7741 agttaggata ggtctgcagc attgtttgga taggttcaac gggcttattc atattcatct
     7801 ccttatagta actaaatact taataccaat aatagataaa caatatcttg tcaagtaaaa
     7861 tatgagaaaa gagtggagaa attattagaa aaaaagaggg aagaatgaag aggggggaga
     7921 ggagagtttc acaaagagga aaagagagaa agagagaatt ttacaaagag gaaaagagag
     7981 aaagagagaa ttttacaaag aggaaaagag aaaaagagaa aataaaatct gcgtgagtac
     8041 cgcgggaaac gaaaatattt aggaaatagg ggctaaattt ccggcgtata tatataggag
     8101 gataaaataa tgattgtaga atccaaattt gaattactct tctgcggtaa ctgcgatgga
     8161 atggtatatt attacaacaa acgcttaggc aaaatgattg ccaggaaatg ggtgaaacct
     8221 aagacaacgg cagcaaaccg gcgtttggga gcaattaccc gaaatttgaa gtccctgaag
     8281 ccatcagaag gttttatcaa agacctgcga gtttatattg cactctatga ttattctccc
     8341 ggcgaaagac attatatgag ctggcaaaat gtatatttaa cgctgatgta tgcgctggca
     8401 aagcaaaatc cagcagttga tttattaaca attacccgag aacaaattac atccgataac
     8461 ctgccctgca aaagtgtgaa agacgctatt gaagcaggac ttctgcgtga ggttaacgga
     8521 tatgagaact tgacgcagga aatataaaaa ccaaatattt aaggagggct ttctattatg
     8581 aaggttaaat tcaagtacgg cattagaacc tatagtggaa ctctggatga gttaaccttt
     8641 ggttcctatt tcaaagaccg cgtgtgtatt gcacgcaggt atgtagttcc tcggcttaca
     8701 gatcaaaacg atttgattgg tagcaagatg aagaacctgg ctgttattta tggagatgtt
     8761 tcggaaagct acaaagtaga actgaaacaa tacgctgcta tgcattccat tttaacaccc
     8821 aaaggaaaac ttccggctac tgcctatgcg ttgtgggtaa aaatgatgtt ccaatttgcc
     8881 agtgcggatg cggaacatat tgacctatca acacttactt attcggattt gcaaaccgta
     8941 ggcgacgaca ttctgtctat ttcctccgct gtatccaacg ggtttatgga taatgttccc
     9001 ggggcggata agctgacggc aaatatgtaa acaagggcgg gacttagttc ccgctttttt
     9061 tttcgcttat tttccacaga tttattttat aaaatattct tggtagtcat tagtcataag
     9121 ctcccgaaaa attatctaac aggcaaaggt tagaaaaggt gctattgatt ccttacttaa
     9181 ttcccgaaga acgacaggga tttagttttt cctctataaa gagagaaaaa tggaatgtta
     9241 attgcattta tgaagatagt cgctttttgc ttagccaagt gcaaagctgg aagtggatgt
     9301 aatataatta ctaacagagg aatagatgac tgttctgata atagtgactt tgatactgat
     9361 aacgcattgg attatagaat atcaaaggca tacaaaaaat gttctgtcaa ttcccattcg
     9421 gattcatatt aacgggacac gggggaaatc aagtgttact cgtcttatag ctgccggctt
     9481 aagagaaggg gggaaagtaa cggtagccaa gaccacggga accttaccca gagttattct
     9541 tccggatgga agagaagcag ctatcttgcg tttgatgggg gcgaatatta ttgaacagaa
     9601 atatatcttc cgtcatgctg taaagcacaa accggatgcc atagttattg aatgtatggc
     9661 agtaaatccg gttttacagt ggataacgga gcgtaaattt attcggagta ctattagtgt
     9721 tattaccaat tgtcgggaag accatctgga tttaatgggt tctactgtgc aaagcgtaac
     9781 tatgtgcatt agtaatacta taccgaaaaa gggaatatgt tacacagcag aagaagaaca
     9841 atatcccctt ttggaaaaag tggcaaaaag cagacaatgt caaattcata agattcgtcc
     9901 tgtagatgtt acggaagagg aattgaataa attcagatat attgagcata gtgaaaatgt
     9961 ccaattagcg ttagctgtat gtgctgcagc aggagttccc cgagatgtag ctttaagggg
    10021 aatgcaaaaa gcgacacctg atcctggtgc cttaaaaaaa tacccaatta aagatagagg
    10081 caaaacaata atattctata atgtatttgc tgcaaacgat cctcagtcaa cagtagaaat
    10141 cattcaaatg gttaccggca atttgcaaaa tatagaaaag attatcattt tgaatagcag
    10201 agcagaccgc ctttttcgta gtcatcaatt agtggatgcg atcaagccct tggatttttc
    10261 ctatttattg ctaacgggag aaatacctga taagatagaa aactatgcta ttcaagcagg
    10321 tatccctaaa gaaaaaatat ttcctttagg tgaaccctta ccggaagtta tctatcaaaa
    10381 agtgtgggaa cttactaagg atgaatctca tattttggga atcggtaata ttgccggaac
    10441 aataaaatat ggggcacaaa tagtagccca cttcagacat aaaatgaaag agtgtaatga
    10501 aaggagccgg atgtgattga tacaatagtt caagccgcgg ttgggatggg tgtgattatt
    10561 tcactgatgt tcagtgaatt acttggtgct tctgcgggag gaatagtagt tccaggttat
    10621 attgctttat atttggatag acctttgcag attttaggca ctttagtaat cagtttatta
    10681 acctgggcaa tcatacgcat tatcggtaat tttaccttgc ttttcggtaa acggaggatg
    10741 gtgctaagta ttttaattgg ctttattttg ggttggcttt cccgtatttt ggtagttcaa
    10801 gatattacaa ttcatcaatt gcagatgcaa tccattggtt acatcattcc cgggcttatt
    10861 gctaactggt ttgaacgcca gggtttttgg aaaacagtat cttctatggg gatagctgca
    10921 atagttgttc gtttggcttt aatggttata tttaacggag aattgtaaaa atgatgtatc
    10981 gtccttcact caaatccaat cgttcgttaa tcattcttct tttattagcc attgtcttgt
    11041 tttatatagc gcagaccagt tatgttaatg taaaaacgga taattatgaa atcaaaatag
    11101 ctgctgcaca gctgatgaaa tcggcaattg attccttatc tgctgaaata aataaaagag
    11161 gaatagaaat agaccctata gatgaccctc tgcaaaccgg tttaatcgga atgcgtttaa
    11221 gttctataac tacggatagg ggtttgcttt cggaaaaaag ggctgctatt aatcccaatt
    11281 tagcggctgt atttgtagaa gaactatcca agatgaaact tatgcccggg gattatatag
    11341 cagtaggaat tacgggaagc aatccagcag ttaatatagc tctttattca gccatccagg
    11401 taatggagct gaatcctaaa ataatagttg ccctatcttc ggcatcttat ggagcaaacc
    11461 gtccggaact tacctggctg gatatggaaa gcattctgaa agaaaaaggt ctctttgatt
    11521 ttggctccag ttatgcctct tttggaggaa aggatgacct ggcaattggc ttatcggata
    11581 atggaattaa agcccttcgg gaagcaatgc aaagaaataa tgttccgctt ttgatcggta
    11641 atgatcttga agataacata gctatccgaa tggcagctta tgaagaacta ttgccggaag
    11701 gaaaacgcta taaagcattt gtgaatattg gagctggttt agctaatgta ggcagcgaac
    11761 ccaatgctaa tcttattcct gaaggaatta atcgcagatt agctgaacat aattacgaaa
    11821 aagagggcgt gattattaaa atggcaaaaa ataatgttcc cgtcttgcat tttcgccgta
    11881 ttttacgctg ggcaaaacgc tataaccttc cgacttcttt tgacacgatg cctaaagttg
    11941 gtgagggaaa aatgtttggt tcccgcatcc ataatcaaac cgtcaatatc atttgtttaa
    12001 ctatcttact tattgccata gtaattgtta ttatttttga ccgtcacgac cgtcggttta
    12061 tggctaatat tgtagacctt gatgaagaac tttaaggaaa ctaaaaaatg cataaacatt
    12121 ttatatccgt cttgctaatc attctgggaa tcaatttgct tttggcacaa acagtaaaca
    12181 ggtataaagt cctgcctttt gataatccag gcacaaaact tctgctcaaa accgaagcag
    12241 gaaactatta ttattatcgc ggtttgccgg aaagacctct aattttaaat actacgggtg
    12301 tagaaaaaat tgaactccgt agtttcagta aggaagcagt tcgtaaaccg gaaattgtaa
    12361 tcgttattaa taaacagaga aagacctatc cactttcttt taaggaaaag aagggaaact
    12421 attatctcta tcaacctctt atgatagata ttccggaaaa cactaaggaa attcagataa
    12481 tatgctatga ccgttcaatt tatatgcgcg ctttcagtgt tttacctcca aaacccccta
    12541 aggcagcaaa acaacctaac atagctatta aagctcatag cggaacagta agtttacaac
    12601 ataacggctc caacagcgat tattacactt ttatgcccaa tcaacccttt aaatttactc
    12661 ttaataatgg cagaaacgcc tatgtttatg tgcgtccgcg tttgcttgac tgttctttgc
    12721 ctaaaatagg attatatgcc aacggggaat tagtggaagt tattgatttt actctcaaac
    12781 gcacgacaaa atacagctgc cagggaatta gcagtttggg aattgccaaa aaaattgttt
    12841 taccggcaaa tgaaaaaagt actgattatg agttgcgcgc tttgagtgac catctgtttt
    12901 tggctaaacc tattttaatg aagaagtaga gctgttttat ggcagataca aatccgaaaa
    12961 tatttcctca agaagcaaag cccatagcgg aattaaaaga aaaagaaact atagcttccc
    13021 ttgattctct ttcccccaag caaacaaagg aaaataagac cgttaaaaaa agcaagataa
    13081 gaagaaaaaa cagtattcgt cctataatcc ttcttcttac ttttttcctt tttttcggct
    13141 tttcccaagt tgaagcgcaa ataaacggtt cagttaaaat tggagctgtt tattcggata
    13201 atgtgtttca tctttcggaa tatgatattg accggtttga tgaccatcat ccaaatctaa
    13261 attttgtaga tactattgat gatttaaccc taaatacccg cattgaactt aagtatccgt
    13321 tatcttatcg ctggtggaaa tttactcctt cagtaagcgg aaatttttcc caaaatatta
    13381 gtaatgaaga taaataccgc actgactgga cattaaaatt taaagttgac cgctattact
    13441 ggaattgcag tgtgcaatat acaagcaatc cctatattta tttccgtcat tttgtagata
    13501 cggatggaac aggtgaactg gaaaaataca gttacagccg agatacttat cgcggtgatt
    13561 tagctgtgaa gccccttccg aaaacaactg tgaaagccaa tctgcgctat gaactttatc
    13621 gctataataa atattttacg gaagctgacg gcaaagccat aaccggagaa gcaggtgtta
    13681 gctacaaatt tccctttttt acagtagagg gttcttatgg ctatcgagat ttcagctgtg
    13741 ataaacgcat agctaataag gattgctcct atcaaagcaa tatttataaa ggagctttaa
    13801 ctctgcccaa aatgcctctt tcggaaaaag gaaaaaccct tattcaacct tcttttgctc
    13861 taaattatga acagcgtttt taccagggaa gcggttcctg gtatggaggc agagctgatt
    13921 atacctattc tttaatggct ggtatggagc tgtttttttc tcctaaaatg aatttatctc
    13981 ttgactactc tcactatttc aggaatgtag agacagataa cgaaacggtg caacgcttga
    14041 aagaatatag tgaaaatcgt ttaagcgctg ttttaagtta caaattctga atttagaggt
    14101 gataaatggt ttttaaaaaa gagaatgatc tccgaaaaat taaggaattc cttactttag
    14161 tggaaaatcc caaagtggaa ttacgccact ataaaaaacc ggttttagtt tgggctgttg
    14221 acgaaggaac tgtcttttat tctttttggg aagaagagat gctcacccgt tttggattgg
    14281 aaaatgtgga cggctgggat ttccaagaag accttctttt ttgtcccgac tggattggag
    14341 atactgaaga agaggattta gaggattttg atttggacga tgaagatatt gaagaactga
    14401 gccatttcct tcatcgcaat atggatgata aggattaact ttgcgcattg ccactcttgg
    14461 aggtggggga tgggggttag cattatcttc tctgctttcc gaaaacgggc atcaaattct
    14521 ggtttgggaa tataatcctg aatatttaat attattaaaa gaaacacatt ctaatccgca
    14581 tttactgccg aatattacgc ttccggaaga agtttgtttc actaacgatt ttcaggagat
    14641 agttgctttt tctccggaaa ttattatttt agcaactcct tcgcagtttc tacgctccac
    14701 tttacaaaag acacctgttt ctttatggaa tagacctgaa cttttagcag tggtaaatgt
    14761 ggctaagggt attgaggaaa ataccttaaa gacacttgat gctgttatca aagatgaact
    14821 gccttttctg gaagagcaca aaatttgtgc cttatccggt ccctcccatg ccgaagaagt
    14881 tgccagaaaa atacctacta ctgtagttat tgccggtagc gatgaggatt tgctctgcta
    14941 tttgcaaacc gtttttagca attcttattt ccgggtttat cgcaatttag accttattgg
    15001 agtggaaatc ggcggtgcag ttaaaaatat tattgctatt gcagcaggaa ttatttacgg
    15061 attggacttt ggagataaca ctatcggagc tttattaacc cgaggaattg tggaaataca
    15121 acgcctggga attgctctga aagctcttcc ggaaacattt ttaggacttt caggaatagg
    15181 ggacttgatt actacagcca cttcacttca tagcagaaac cgctttgtag gcgtggaaat
    15241 aggaaaggga agaaaacttt ccgatatttt agcgcaaatg gaaatggtgg ctgaaggagt
    15301 aacaaccacg cggagcgttt atttgctgtc ccgaaaatta aggatagaaa tgcccatcgt
    15361 agaaaaggtc tatcaagtcc tttacgaaga taaagaaccc cgaaaagcaa tcttagaact
    15421 gatgacccgc gaacttaaag ccgaataaaa atccacagat gaaggaaaaa tagttttatt
    15481 tcacgctgat ttcgcagatc tcgcagattt tatttttaca aagagaaaag agagaaagag
    15541 taagaatgaa aaatttagaa tgataaatgt agaatgtaga atttggaatg taaaattgag
    15601 aattgacttt ccggaagaac gatatccttg tcaacctctc ctaaaaaagc ttgagaaggg
    15661 ttgaaaaggt tgagtgggtt tgtagaggtt taatggttga gatggttgag aaagtttaga
    15721 aagttgagag ggtttagaag gttgagaggg tttagaaggt ttatatggtt gagatggttt
    15781 agaaagttga gagggttgag agagtttaga gggtttataa ggttgagatt ccataaaagc
    15841 ccggagggag acacaaggat agcgatgggt gcgtaagccc ctcgtagaaa tgaaagcccc
    15901 gagttttttc ttttttcctt tcctttttaa aaaagcccct tagggcgaca caatgatgta
    15961 gaaggtggcg taagcccctc gtaaaatttg cgaagtaccc ctgggaagtt ttggcttaca
    16021 attttcacct gccttcaatg aatacacaac cttctatcta ttacacttac aaccgagctt
    16081 gtactgattt ccgaattaaa aaattgtgag ctaaaacaaa aagcagaatg cgaaaaaaag
    16141 aaatagttct tgttttaact cacaattttg aaatgaggag accagtaata atttggttgt
    16201 aagtgtggat aaattgatgg tttttgtttc tgtgagggca gtggcaaaat tgtaagtcaa
    16261 aactttctcc ccgttgaaaa ttgtgagcta aaacaaaacg tttatctggt ctaaaaattg
    16321 cttttataaa agaaaagaaa caaaacagag gaggagataa tgctgcagaa gtttttaatg
    16381 agcgtagtga ttctaatgtg cgtctatagt tctctatctg ctcttcgcta tacaactttg
    16441 gaggattttg aaagtggttt tgtttcttta agctcctgga gcattgaaga ggatttgcaa
    16501 ccagatgcct ggagcttaga tacttctact cccgatagca gttcctattg tcttaagtta
    16561 actggtaatt gttataaatt acagcaaata actccttatg ctattgacag cacaggtgta
    16621 attcaggttc aagtaaaaac cagttcaaac ccaaggattc aaggcatcgg ctttacagat
    16681 ggtgtgcata acattcttta tagttttgcg gggagtagaa ttttagatat tgaggtttgg
    16741 attccggttt atcaaggtgc ttttagtaac ggtgtctgga atacctttca attgcctctg
    16801 gcattggatt ggcaggcatt ttggggttat cttcctgtta tcaacggcat aatttatatt
    16861 aacgatttgg atggaatttc ctctcgcagt gtgtggtttg acaatataat agacatcagt
    16921 tccgaccttc ctgttccacc aactgtaagc atatcaactt catatcctgt aaaaaatacc
    16981 ggggtttatt tctatagcaa tgttattgac cctgatagcg atacatttac ttatgaatgg
    17041 tcttttggcg attccacttt cagcaattta cccaatccct atcatttata tacaattgct
    17101 tccgcttatc cctatacagt aacactgaaa gtgactgatg atacgggaaa agttggtttt
    17161 gcttcaacgg aagttacatt ggaaacaggt gcttcccatt tgcctgtaac ccttaatttt
    17221 gtaggggaca taatgcttgc cagaagatat gagaatcccg gaggtataat tcccacttta
    17281 ggagttaatg ccattttttc gcctactaaa ccctattttg gagatgcagc ggatattagt
    17341 gtggcaaatt tggaagttgt tttagctaat gtgggagttc atcatcctac caaaagtgtt
    17401 tattacagag gcaatccggc taatgtaagc ggtcttgttt acgcaggaat tgatgttgtg
    17461 agcacagcta ataaccatac tatggattat ggcattgaag catatcagca aatgcagggt
    17521 ttgctaaata actcgggaat taaatatagc ggttgtgggg ctaattctta cgaggcatat
    17581 ttacctacct tttaccattg ccgtggtttg aatattgcct ttttacgtag ttcggacaga
    17641 acaggacaat ataataatgc tcaaccgttt ttacaagctg gatacaataa accgggtttt
    17701 gcttatatga caccttacta tgtagaacag caacttcaag cagttgaagg aattgccgac
    17761 cttaaaattg ttgaaatgca cggaggtagt gaatattcta ttgctccagg tgccggttat
    17821 gataaggaat tcaatccctt tttagaggat acggaagatg aggactattt ctatcggaat
    17881 gatgttcctc atcaatggga tatagaaatc aggcacagtg ctattgaaag tggtgccgat
    17941 ttggttatag ttcatcatcc gcatattatt cagggattgg aattatatca aaataagctg
    18001 attgctcact ctctgggaaa ttttgttttt gatttggatt atccagaaac gatgcccaca
    18061 atgattttgt atgcagatgc ctattttgac ggctttagga actttcgcat aaaaccgttt
    18121 tatatagacc aatacattcc aaaaccggca acagggcaat tggctgttta cattttggat
    18181 tatattgcca gcaggtctat ggattttaac acgcgcgtta tggtggataa agaaaatctg
    18241 gaagcaagag ttttaatgga agatgaaaat ccacccgtta ctacaaatgc tttcagttcc
    18301 tggcaacatt tactgtttca cagcgggggg atgagttata cttcacctat taaattaccc
    18361 cggagaggtt ctattagcaa tattattaat gttgaaccgc ctgctgaata tcaatttcgc
    18421 ttaggtcaag aacatatctg gtttggtaat tttgaaaatg agggctcaac tctttggatt
    18481 ccacctgctt attctacaac ggactttata gatggagagc gtagtgcaaa actgactact
    18541 accggagcag gttcagtaac ttctacaata tcccagcgtt gtaaaatcta tgataatacc
    18601 aaaaaataca ctctgcacgg ttggctgaaa accgaaaatg tatctcttgc caatatttcc
    18661 atcagatttt atacatccag aacagcagca gttgcgtcct atacggatta tgtaacggaa
    18721 aacattatag gaacaaacga ttggacatat tactataaag aattgaacct tcctaccgat
    18781 gttttctatt atgaaatcca tctacaaatg actggcagca caggaatgac agcaatcgcg
    18841 ttatttgaca atgtgggttt gattgaatgg acggaatgga ctgctacagc ggacttaatt
    18901 aatataccct ggcctaataa ttattactgg attcaagcgc gaactgccga taccccaaag
    18961 tcaatgagtt tttccctcgt agaacgcagt tttggagttc aacgagcaaa tgcctcaaac
    19021 catttttcca agatgaaacc ggaattaaat atcagcccca atccttttaa tcctgaaact
    19081 ataatatatt gtgttttacc tgtatctggc aaaactacta taaaaatata taatattaaa
    19141 gggcaattgg tgaaagaatt gtataaagga gaactggaag cgggaattca tcgcttcaat
    19201 tgggatgcaa aggatattga caaccgttcc gtcggctcag gagtttattt catttacctg
    19261 gaaaatggca gtagtaaggt aattcgtaaa gcagtcctaa tgaaataaaa gggaattttc
    19321 ttgacgaaat ttacctctaa taataaaggg ttaaataata aaacaaaatt aaggagctga
    19381 aaagtgcgta aatttctttt aaccttagtt ctcctggtgc tggctttttg ccttatggct
    19441 gaagaagcag acacgccaaa gataaatttt gccttactaa atcctaaaga aattaatctt
    19501 gaactacgtg tctatgctcc tatgcaaaga gtttatatta cagcggattt ctccgtggaa
    19561 aaggattctc tacctgacgc agtgtatcat agtttatttt taaccaagga tgccagaatt
    19621 gagtattttc tgattaatga taagtttgtt cctcctgttt attctactaa actcgttccg
    19681 gaacatttta atcctgtgtt tccctatccg gaaatgctga atgaagatag taacttgaat
    19741 tgtttcagtt tttatctgcc aaactacgaa ggcaaaataa aattcacttt acgctattct
    19801 attcctattc cggaatgggt gaaagaatcc gatattcgtg gctatgttac tttctcttcg
    19861 gctcaaaatt ggtatccacg aaatcttaca ggtccttgta aggtctatgc acgtttgatt
    19921 agttcaacct attatacaat ggaactcgga actcaatgca cgattaaaga aaaggatgat
    19981 atccgcataa ctgagggata ttttattgat tctcccgcag aacaatcaat ttttaaaatt
    20041 attaaaagct aaattagctc agctggtaga gcaactgatt cgtaatcagt aggtcggggg
    20101 ttcaagtccc ccatttagct ccatttgtta caaagagaaa tagtgagagg gttgagaggg
    20161 tttagatggt tgggttcttg ttttaactca caattttgaa atgaagagac tagtaataat
    20221 ttggttgtaa gtgtgaataa attgagggtt gtggtttctg tgagagcagt ggcaaaattg
    20281 taagtcaaaa caagcaggaa accatttgta gtgcggttgg aagggtaaaa gcaagaggtt
    20341 ttatgataat tgtcagagcg agtgaaaatt gtgagtcaaa acttcatctc gggtgaacat
    20401 tgtgagtcaa aactttccag ttttcatttc cgtgagcggt atttttatac ttggcgtgtt
    20461 cctttgaggg ag
//
LOCUS       DAOPFL010000024        19735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2972_length_19735_cov_97.626220, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000024 DAOPFL010000000
VERSION     DAOPFL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 19735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19735
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2972_length_19735_cov_97.626220"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1645
                     /locus_tag="PLG69_03140"
     CDS             302..1645
                     /locus_tag="PLG69_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPI42535.1"
                     /translation="MKNVKTIALNQLINYIANDPLNKLPKIMELAKKLDRKGTYLKLF
                     TVITDALSDETSHWHKFVENLFNNVDSKVIHKLAECFAINTTLVGRPIREKYKAKYSI
                     QVPWAILMDPTSACNLSCTGCWAALYGKQHNLPFEVMDSICKQGKKMGIYWYLFSGGE
                     PLIRKNDIIKLCKKHQDCFFLSFTNGTLVDEKFCEEVLNAGNLTLCFSIEGNEPATDM
                     RRGKGSYKRVIEAMELMKKYRLFFGYSTCYHRYNTESVASDEFVDDMIARGCFYAWNF
                     TYIPIGKNAQPDLIATPEQRAYMYKRVNEIRTHKPILALDFWNDGELANGCIAGGRSY
                     FHINAAGEVEPCAFVHYSNVNIKEVSLLEALQSPIFKAYQKRHPFNQNLLQPCPMLDN
                     PEKIMEIVNEANAKSTDIEAPESIEELCAKTKPAAEKWAPVAKELWENREKNSST"
     gene            1979..2869
                     /locus_tag="PLG69_03145"
     CDS             1979..2869
                     /locus_tag="PLG69_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPI42536.1"
                     /translation="MIKEVGRLNLDDYKILDTEVEKIFRNIEKGKDALAEKQITDLGN
                     TANYFVREELGKRLAQYSGNGEMDAICGRLLDHFIYGVRATALFYFYYKHQDHPEIII
                     KTVEKTFESVPWESETICFELWKKAPNVMQEYMPAWAESDNEKKRAISMHGMENIASR
                     HPQYVLSFVSNLLDDESEEVQKKVCHILTQVGRQKPLQCYNSIRRWLIEGDDTRIRIL
                     WQTMKKLANIYAQRSRRDKTNEFMNVTQKTIAEWRNDPDPKVATMGAKLSGIIAKKKK
                     RENQNQKAANSENNYYSRQI"
     gene            2866..3702
                     /locus_tag="PLG69_03150"
     CDS             2866..3702
                     /locus_tag="PLG69_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPI42537.1"
                     /translation="MNIRQDFSRIAVILVEPIYGGNVGAIARIMHNFCFSDLRIVGKV
                     PEKNDFYLAMHSEEILENVNTYSTLEDAINGLDRVIAFSRRLGKNKPVDFTPGQMAKY
                     VHSLPDSKIGLVFGRETYGLTDSEAELCPLRCHFMANPSFPSLNLAQAVALALWEIYS
                     LPWEEKQKGKKYYNSASGKELDEIRNYILEVMRQCGFFQRRETINWDAFLAKMLAQLN
                     PDKNMLYRLRQMFNRFNVLVTGQGLGYELPNSSSGKEENKKNSELDNSCSTSSRPCNP
                     DT"
     gene            3724..6453
                     /locus_tag="PLG69_03155"
     CDS             3724..6453
                     /locus_tag="PLG69_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42538.1"
                     /translation="MKRTLCLVLLCLPLLILATTLKTTIQFPVPQEGLSQSALFKQGY
                     GFSSAPGTLQLPVKQINILLPKDAVVDNWKVILEAEATFTEEPPAINSAFSNGEEILT
                     SPVNRNIYSRYRYMGLRKWGELNYACFNVLPAIYNGSHWLWSPSCQITINYTSSAKAK
                     GIIPPTYTDAGFFANPQYLSQWYTTSKEGNNRLLVITTPELYTALDWLVTFRQSQGVD
                     VSFCDIALALASGTGTDNAEKLRNYLQNTYASTPFSYLLLVGDYDTVPVAYVTPEPNG
                     QETVPTDFFYADLSSNWDTDNDGRRGEYSTGYMNEDYGIDFTPEVFVGRISTNNPTNV
                     SAIANRIVAYEQATEPWKEKNLLPAAFLNYQGEPEPVYLQTDGALFMEFMRNTCLAEQ
                     ENFTMYEQEGVVPSFPCDLPVNYDNLINKLNSESWGFINWDAHGSSTNSSRKVWINDY
                     NQNNVPDENEMQWMNLVNRQSFDNLENTSGTIIYAASCYNGALDSNNYCLAEYALIKK
                     AVGVIAATRTGWYKVGWQNPGWGGLASYDYHFVENFRQAKLDLGSAHSWANLLHTQYY
                     LFGDPVDNGGIIYPELQNVYTYMLFGDPMVGWTPNQISPAGEILVWEPEGNEGLAVVN
                     ALRKISNFNVIYSDKLIPDYDYLNNFEAVFYLAGNSLPETELEPTSYEYSYLNNYLEA
                     GGKLYCENYIDDSYGELYTKMGVQMADNNPLNITSIFYPSSNLAWNYNGADSSVYPII
                     PAQTTAQGIFFASCGNTNNPVIGILNATDNYSVLTSSFRTTQIESGEYTLNNMIGIIL
                     SELNVMDYNPVGNNDPIMVPVIQSVSVYPNPASQSSSISFNLTKSAPISIAIYNIKGQ
                     KVKDLIGSELKSGNYQFTWNGKDNNGRYCSSGVYYYRIVTPEKNITKKMLIFK"
     gene            6866..7690
                     /locus_tag="PLG69_03160"
     CDS             6866..7690
                     /locus_tag="PLG69_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPI42539.1"
                     /translation="MILRKIKCILWHIYLISEFFLVSAIIRIFSADPVLRRKRLLKNN
                     TRISKRFIRALNIKLTLNNSENLQKLQDIPYLAVSNHTTYLDIILLSAVENFVFITSV
                     EMRKNPFLGRITKSGGCLYTNRKKYISLPTEIEKFASAIHQGFKVVLFPEGTSTNGIT
                     VQPFRRSLFQVAIEAKCPILPVCIKYKAIDGKKIDKKNRDLIYWYGDMPFLIHYLKII
                     GHSLEAQIDILTIIPYQEGKTRQELANQVYGQILSSYLEQESSVDKQSNSLSMVLK"
     gene            7724..7924
                     /locus_tag="PLG69_03165"
     CDS             7724..7924
                     /locus_tag="PLG69_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42540.1"
                     /translation="MNNKDDEKNKLHLNRTNFILLLVAAIVLVVGYIIMSFNEITISP
                     ILLVIAYAVIVPFALLYHPKKK"
     gene            7926..8804
                     /locus_tag="PLG69_03170"
     CDS             7926..8804
                     /locus_tag="PLG69_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPI42541.1"
                     /translation="MPLLSEEALVRLNKFQLTAKSIVEGFLVGLHKSPYHGFSAEFSD
                     HRQYNPGEPLKDLDWKILAKTERYYVKRYEEETNLRSYILLDHSKSMFFSSGKSTKIE
                     YATQLAGALAWLMISQKDAAGLYTFNNKITSAYPPKAIRSYTSQLFAALLDLKAEDQT
                     DLLTPLHQIAELVKKRSLIIIISDLLDEPERIMEAFKHFRARKHEVLVFHIIDPKEEK
                     FDYKREAQFIDSETGEKITVSPWQIRKEYVENFHQFAEYLKRECYKNQIEYNPVDTYT
                     PIEDLLLKYLIKRQKG"
     gene            8808..9659
                     /locus_tag="PLG69_03175"
     CDS             8808..9659
                     /locus_tag="PLG69_03175"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPI42542.1"
                     /translation="MAYRYVSHETIRSELANALKYITFNPDPELPALLKNALANETSE
                     ISKDILKCMLKNIELAPKIEIPLCQDTGSLVVFAEIGNHCIIDGPPLPEIINETLIIS
                     SAELYLRPSILKDPLLQRENTGNNAPAVIHISIVEGDKLKLQIAQKGGGAENMSQLKM
                     LIPSAKADEIKNFVVETVTLAKGNACPPLIIGIGIGGNFETCALLAKKALFQPLTAKN
                     PIERYALLEQEILKGVNETGIGVQGLGGKTTALAVHILNAPCHIASLPVAVNLQCHSH
                     RHIEIVI"
     gene            9663..10166
                     /locus_tag="PLG69_03180"
     CDS             9663..10166
                     /locus_tag="PLG69_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPI42543.1"
                     /translation="MKTYRFTLPLCPADIAQLHISDKVLLTGCLFTARDVAHKRMVDI
                     LKRGEKLPLNLAETTLFYCGPSPARPGNICGAIGPTTSSRMDIYTPLLIQNGLRVMIG
                     KGERSIEVQKAIKNFGAIYFVMPGGISAYLSQHIVSCETFLWQELGPEAIHKLWVQDI
                     PVYVAIK"
     gene            10436..13363
                     /locus_tag="PLG69_03185"
     CDS             10436..13363
                     /locus_tag="PLG69_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42544.1"
                     /translation="MRLFKSRLIFLLLFLLLLLSTVIIAQKQSDAERYIVEDFKVEDI
                     PADDGTGLMLSWKPLERDKRIIEYRIYRGVSPDTLFFLDAIPVNVKTGVASERMFYYD
                     NSGSEFFDISSPRKLKKEKQQGKNSPLYQKMPRNIPFLAEISSKFSLLSVSEKNKYYY
                     SSQKVTLDKPQEQGSEKEKQEEGETYAGLKDNQQTVYCFLNPGENYYYTIVAVNERRQ
                     FQKHAKITAGSPKPNAPEPATALHSVVLEDSRELRFEWELPLYNSDIAQYRIHQTPLF
                     TPTEWDSLRLHPELFQTKTVILQSGLAGYGSGKNYSQVSIPEEASLEAYINSAYTLEL
                     IDADGFSSFSALSKPRITHSKDLPAKPIIKMEDKPNDKGDRLTVVWDHPICFVVKTTA
                     LNSKFNKFRVNYQLNKTETQQVDNIYFEFYKKGEEKPFAKINEFYQDNSVILKVPNNY
                     DYKKGFRVKITLAGKPEIPKDYCIEQDLEYDPNMLAILPTKALYRNGKDVSKISNVVY
                     RKNMASKQLTLVKRNTSYDNSLDVTIPYATMIQKPVNGFTFAQGDTLITYINGERQAR
                     KLKPGDIKTPLALISPNIDLVYDKENETRIETNIFPDLGRKEAQEKVEELTKKIAELK
                     DKITGETDPAVVEQLNQNIEQLQKQLNAYSKNEHLVNANKITNRKSRMRYIAKVREDY
                     SRYSTYQVVRTNSRGLFTESDLLKDGDDIFYMKPVSNVFDTNKYTTLVAMLIFGLMVV
                     IFINLARRGKDLYIRPIAGLEEIDTAVGRATEMGRPIMYMMGYGSLGDVATIASMGIL
                     SLVAKKAAEYDIKLIVPVYNYIVMPVVQEIVRDAHYSVGRPDSYDKNSVFFLTDVQFA
                     YVAGVNGIMIRERAATNFYMGYFAAEALLMTETGNGIGAYQIAGTDAITQIPFFITTC
                     DYTLIGEELYAASSYLNREPMQLGTLKAQDYYKFLIFAFVIAGAVLSTFQLTGLSELF
                     PLK"
     gene            13376..14005
                     /locus_tag="PLG69_03190"
     CDS             13376..14005
                     /locus_tag="PLG69_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42545.1"
                     /translation="MKKTIPLIIVIVGGIIFVLYALSPHKLLQDDFYNKFYLPWVYAS
                     SPWAALLGVISLCMMHSSKIKRKAPKWQYSYFFFAGFIITSLAGFIGGTQKGSVLMWI
                     FENMQMPMSSTMFSLLAFYMASAAYKAFRARSVEATVLLLAAIVVMLAQVPLGVKIYK
                     NLPIISQWILDVPNLASKRGIAIGVGLGGVATSLKILLGIERSYLGGTD"
     gene            14024..15205
                     /locus_tag="PLG69_03195"
     CDS             14024..15205
                     /locus_tag="PLG69_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42546.1"
                     /translation="MNFFERMQSLDRRWIYIIVALAIIIPLIIPYNSDNVTSPPTENL
                     YQMIDSFAGREDRAVLLSFLHDAATMPELYPMEIAIIRHCFERNIKVFALTFLTSGAP
                     IIDYAFNSVKEEYPDIKSGVDYCNFGYKPQPMAVVLGMGDNIANAVNTDAEGRKLESL
                     PIMKGITNYNEMNLVVEFSGSSPGVYWIYYARPKFGVNVALGVTAVMAADEYPYLQSG
                     QLIGMLTGLKGAAEYEKLVDVFAAYRDPAIDYSVKTDAEGNKILPGRPFGKEVLNDES
                     TKKLINITTQTKAEFTPEEYTAFVAKYPEQKAIFDQLKEEQNGTIIIDVTKITPELRN
                     QMGETAYREINRLTHNISYKFKVARIGMNAQSVAHIMIIIFILLGNIGYFIQKAKTAE
                     K"
     gene            15222..15824
                     /locus_tag="PLG69_03200"
     CDS             15222..15824
                     /locus_tag="PLG69_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42547.1"
                     /translation="MSFELFSNLVAAFLTLCIFSFLYKENPFYRMAEQLLVGVSLGYT
                     LVFTYERIFIPYVWQPIILKHNFVLILPCLMGILYLFRFSRKLGWLSRYPIAFSMIGI
                     GFGVPMGIHAGILVQMRQAMVPLENINLILILLGTIAVLLYFFFSKAHTGAYGKFVGL
                     GKWYMMIGFGASFGLTVMARISLLIGRIQFLVIDVFGLMK"
     gene            15933..18722
                     /locus_tag="PLG69_03205"
     CDS             15933..18722
                     /locus_tag="PLG69_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42548.1"
                     /translation="MKKYLIVIGIILLIGISLLSTDDAKPASLKGKITEFSASDMPFD
                     DGSGIVLKWKPLDKSYRIINYNIYRGVSPDSLFYIGSIEVDPKLGVLSPELYFYDKGE
                     EPFIEFETAPQKLKKEKQQSHNSPLFKKFPQQPELLSTMLTRYNIIGAINNSKLYHSS
                     KPVTKDEDIMAGLKLNRFETILGFPKAGQEYYYTVLAVNERGKYLPYADIQKVIPEDN
                     PPEPSANLSSTYIQDTGVFNFEWLPPSSSEDIAMWEGWLIPLEQKPNNGELLSENWQN
                     SAIPLFQIPNYSGAVNTCYSVDTKEEGINLPANLADYYPVLSYSDYMGQSAAVSAKNF
                     RILNSSQQLKIPQIEVKDKPNDKGDYLSVSMGKPLVFVSQVLYLNKAKTALRINYEIS
                     NNEHYKIKELRFTVLDSLNKEIGKVSEYYLDKTIHFKLPQQYIAIKNMKIRIAVQTNG
                     NGSFEDYTEQHIVYDEGNKIFRGENIYYQNEPVNKLYYEILTRNGFDPDFLFGERTNG
                     LTRAYDHSIPYESIVYKSILDYDNKGKRLILDPQITIAVDQVNGYTFAVPLFRNKFEQ
                     DLKKQKEELDNLLAEKNKYPANAIPDSLLAAIQEAEGNYNFITKHPAYLQAQKTKTNK
                     EWLKTLLATHNKNMRSYSYKVIKTDGKANFVTTAIYADAKGNTTFYPRSDWFDTTKYI
                     TLAASILLCLFLIYAIIQSRKGNAYIRPIAGLESIEEAVGRATEMGRPIMFVPGWGTL
                     GDPDTVASMLILGQVAKKAAEFDVRLISPHCDYMVLPLAQEIIQNAYNEVGRPDAFNS
                     NDIFFISYDQFPYCAGINGITVRERVATIFYMGYFNAEALLLTETGNQTGAFQIAGTD
                     AITQVPFFITTCSYTLIGEEFYAASAYISRNPDLVSMLKASDYFKAVIVIVLLIGTLL
                     ATLNFTGFINAFPIE"
     gene            19173..19622
                     /locus_tag="PLG69_03210"
     CDS             19173..19622
                     /locus_tag="PLG69_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42549.1"
                     /translation="MKATRANLLGEFTGHLDGLIYYRNKKNGKLYVRRRFKFKNHPAH
                     PVFTKNIQNIYALSPSEGYKQNLKIYIALFNANLPEGKKPLHTWTNAYTTLMFALQKA
                     FPETVNLGTLTRDQIYSQNLPCKSVKQAVEAGLLPLVKSYELLSAEF"
BASE COUNT         6361 a         3592 c         3873 g         5909 t
ORIGIN      
        1 cctgatactt gtcattcttg tgaaagcaga aatccatact attaaataca ttaaaacaga
       61 ttctgcatca agtgcggaat gacaagctaa ttaagctcaa ccatttttct cattcaaata
      121 aatgatatca accgaacatt gtggagaata aaggaagaat taaactctgg aataaaagat
      181 aaaattcctg tttttgactg aaaatgtgaa cctttacaat actcttataa aaaaatactt
      241 gacattttag aagaagaatt ttatttagta aaaaaaaagg tatgactttg ggtgatttgc
      301 tatgaagaat gtaaagacaa ttgcattaaa ccaattaatt aattacatag ccaacgatcc
      361 tctgaataag ttgcctaaaa taatggagtt ggctaaaaaa cttgaccgta agggaactta
      421 tttaaagtta tttactgtaa tcactgatgc tctgtcagat gaaaccagcc attggcacaa
      481 atttgtagaa aacttgttta acaatgttga ttccaaggtt attcataaac tggcagaatg
      541 ttttgcaatc aatacaacac tggttggtag accgatacgg gagaagtata aagcaaaata
      601 ttcaatacaa gtgccctggg ctattctgat ggaccctaca agtgcctgta atctttcctg
      661 cacaggttgt tgggctgctc tatatggaaa acagcataat ctgccttttg aggttatgga
      721 ttctatctgc aagcaaggca agaaaatggg catttactgg tatctttttt cgggaggcga
      781 acccttaatc cgcaaaaacg acattattaa actctgcaaa aaacatcagg attgtttctt
      841 cctgtctttt accaatggca cactggtgga tgaaaaattc tgcgaagagg ttctaaatgc
      901 aggaaattta acactttgct tttccattga aggaaatgaa ccagcaaccg atatgcgcag
      961 aggaaaagga agctacaaaa gagtgataga agcaatggaa ctgatgaaaa aataccgttt
     1021 atttttcggt tattccactt gctaccatcg ctataataca gaaagcgttg cttccgatga
     1081 atttgtagac gatatgattg caaggggctg tttctatgcc tggaacttta cctacattcc
     1141 tatcggcaaa aatgcccagc ccgatttgat tgctactcct gaacagcgcg cctatatgta
     1201 taaaagggtt aatgaaatca gaactcataa gcccatcctg gctttggatt tttggaatga
     1261 tggagaactt gctaatggtt gcattgccgg tggacgcagc tattttcata tcaatgctgc
     1321 cggagaagtt gaaccctgtg cctttgttca ttactccaat gtaaatatca aagaggtctc
     1381 tcttttggaa gcattgcaat caccaatctt taaggcatac caaaaaagac accccttcaa
     1441 ccaaaacctt ttacaaccct gcccgatgct ggataacccg gaaaagatta tggaaattgt
     1501 aaatgaagcc aatgccaaat ctacagatat tgaagctccc gaaagtatag aagaactgtg
     1561 cgctaaaaca aaacccgctg cggaaaaatg ggctcccgtg gcaaaagaac tctgggaaaa
     1621 cagagaaaaa aatagcagca cataaatttt tatccaccga gaacaccaaa ttcatttata
     1681 ataatctatg tcgtcgtcat aagtgctttt aaagttattt ctatggtcaa agttttgaag
     1741 acgctaatta ctcctgactt tcctcttgac tttttccttg acagatagct ctcttataat
     1801 aaaagggtat cagctgtaga tacactttaa tcccatttat cttccgttcc ttaaataaag
     1861 ggtaaacaag ggaagggtat taattagttt atgtcaaaca agataaattt acaagtgaca
     1921 aaaaaaacac tatttaattt agtgacggaa cgaaaatatc cgttacggag cagggtaaat
     1981 gataaaagaa gttggacgtc tgaatctgga tgattataaa atcctggata ccgaagtgga
     2041 aaaaattttc cgcaatatag aaaaaggtaa agacgcactc gcagaaaaac aaattactga
     2101 tttgggtaat acggcaaatt actttgtccg agaagaatta ggcaaacgct tagcgcaata
     2161 ttccggtaat ggagaaatgg atgcaatttg tggacgcctt ttagaccatt ttatatatgg
     2221 tgtaagggca accgcactct tctattttta ttacaaacat caggatcatc ccgaaatcat
     2281 tattaaaaca gtggaaaaga cctttgaaag cgtgccttgg gaatcggaaa caatctgttt
     2341 tgaactttgg aaaaaagctc ccaatgtaat gcaggaatat atgcctgcct gggctgaatc
     2401 ggataatgaa aaaaaacgcg ctatcagtat gcacgggatg gaaaatattg cttcccgcca
     2461 tcctcaatat gtgctttcct tcgtttccaa tcttttggat gatgaaagtg aagaagttca
     2521 gaaaaaagtt tgtcatattc tcactcaggt aggaagacaa aaacctttac aatgttataa
     2581 tagtattcgg cgctggctga ttgaaggcga tgacacaagg atccgcattc tctggcaaac
     2641 aatgaaaaaa cttgctaata tttatgcgca gcgcagtcgt cgggataaaa ccaacgaatt
     2701 tatgaatgtt acacaaaaaa ctattgccga atggcgaaac gatccagatc ctaaagttgc
     2761 cacaatggga gcaaaacttt ccggtattat agccaagaaa aagaaacgag aaaatcaaaa
     2821 ccagaaggca gctaattccg aaaacaatta ttattccaga caaatatgaa cattaggcag
     2881 gatttttcac gcattgcagt aattttggtg gaaccaatct atggaggaaa tgtaggtgcc
     2941 atagccagaa taatgcataa tttctgtttt tcagacctgc gcattgtagg taaagttcct
     3001 gaaaaaaacg atttttacct ggctatgcat agtgaagaaa ttctggaaaa tgtaaataca
     3061 tatagcactc tggaagatgc aattaacgga ttggatagag ttattgcctt ttcccgtcgc
     3121 ctgggtaaaa ataaacccgt tgattttacc cccggacaaa tggcaaagta tgttcatagt
     3181 ttacccgatt ccaagatagg acttgttttt ggacgagaaa cttatggctt aactgattct
     3241 gaggcagaac tttgccctct gcgctgtcac tttatggcta atccttcctt tccttctctg
     3301 aatttggctc aggcagttgc tttagcactt tgggaaattt attcccttcc ttgggaagaa
     3361 aaacaaaaag ggaaaaaata ctataattct gccagcggaa aagaactgga tgaaatccgc
     3421 aactatattt tggaagttat gcgccaatgc ggttttttcc aaagaaggga aactataaat
     3481 tgggatgcat ttctggctaa aatgctggca caacttaatc ccgataaaaa tatgctctac
     3541 cgcttaaggc aaatgtttaa tcggtttaat gtattagtta cgggtcaggg tttgggttat
     3601 gaattgccga attcctcttc gggaaaagaa gagaacaaaa aaaattctga attggacaat
     3661 tcctgttcca cttcctcaag accttgcaat cctgatactt aaagatatta taaaggaaga
     3721 aaaatgaaaa gaaccttatg tcttgtgtta ctatgtttgc cgttgcttat tttagcaaca
     3781 actctcaaga caacaattca gtttcctgtt ccgcaagagg gcttatctca atctgcatta
     3841 tttaaacaag gttatggatt ttccagtgct cccggaactc tacaattgcc cgttaaacaa
     3901 ataaacatcc ttctgcctaa agatgccgtt gtagataatt ggaaggtaat tttagaggca
     3961 gaagcaactt ttacggaaga gccgccggct attaattctg ctttttccaa tggtgaggaa
     4021 atattaacat ctcctgttaa tagaaatatt tattcccgat acaggtatat gggtttaagg
     4081 aaatggggtg aacttaatta tgcttgtttc aatgtattac ctgctatata taacggttcc
     4141 cattggctgt ggagtccctc ctgtcagata actattaatt acacttcttc tgccaaagct
     4201 aaaggcatta ttcctccaac ttataccgat gcaggttttt ttgctaatcc gcaatatctt
     4261 tcccaatggt atactacatc caaagagggc aataaccgtc ttttagttat tactactccc
     4321 gaattgtata cagctttgga ttggcttgtt acatttcgcc agtcccaagg tgtagatgtt
     4381 tccttttgcg atatagcttt agctttggct tccggaacgg gaaccgataa tgctgaaaaa
     4441 ttgagaaatt atctgcagaa tacttatgcc tcaaccccct tttcctattt gcttttagtt
     4501 ggtgactatg atacggttcc cgtggcttat gtaactcctg agccaaatgg gcaggaaaca
     4561 gttcctaccg atttctttta tgcggattta agcagtaatt gggataccga taatgatggc
     4621 agaaggggtg aatattctac cggttatatg aatgaggatt atggaattga ttttaccccg
     4681 gaggtctttg tgggacgcat ttctacaaat aatcctacaa atgtttccgc tattgcaaat
     4741 cggatagttg cctatgaaca ggcaactgaa ccctggaaag aaaaaaactt gttacccgca
     4801 gctttcttga attatcaagg agaaccggaa ccagtatatt tacaaaccga cggagccctt
     4861 tttatggaat ttatgcggaa tacctgttta gcagaacagg aaaactttac tatgtatgaa
     4921 caggaagggg ttgttccttc ttttccctgt gacttaccgg taaattatga taatttaata
     4981 aataagctta attctgaaag ctggggtttt atcaattggg atgcacatgg cagttctaca
     5041 aactcttcac gcaaagtttg gattaacgat tataaccaaa ataatgttcc tgatgaaaat
     5101 gaaatgcaat ggatgaattt agttaaccgc caaagttttg ataatttgga aaataccagc
     5161 gggacaatta tctatgctgc atcctgctat aacggagctc tggattccaa taattattgc
     5221 ctggcagaat atgccttaat taaaaaagcg gtgggagtta ttgccgctac cagaactggt
     5281 tggtataaag tcggatggca aaatccaggt tggggcgggc ttgccagtta tgactatcat
     5341 tttgtggaaa acttccgtca ggcaaaactg gacttagggt ctgctcattc ttgggcaaat
     5401 cttttgcata cacaatatta cctttttggt gatcctgttg ataacggagg aataatctat
     5461 cccgaattac aaaatgttta tacttatatg cttttcggag accctatggt gggctggact
     5521 cccaatcaaa ttagccctgc aggtgaaatt ttggtttggg aaccggaagg aaatgaagga
     5581 cttgcagttg ttaatgctct caggaaaata agtaatttta atgtgattta ttccgataaa
     5641 ctgattccgg attatgatta tctgaataac tttgaggcag tgttttactt ggcaggaaat
     5701 tctcttccgg aaacggaact tgagccaact tcttatgaat atagttactt aaacaactat
     5761 ctggaagcag gaggtaaact ctactgcgaa aattatattg atgatagtta cggtgagtta
     5821 tatactaaaa tgggtgttca gatggcagat aataatcctc ttaatattac atctatcttc
     5881 tatccttcca gcaacttggc ttggaattat aacggtgctg attcctctgt atatcccata
     5941 attcctgctc aaacaactgc tcagggaata ttttttgcct cctgcggtaa tactaataat
     6001 ccagttatag gcattttgaa tgctacagat aactactccg tgcttacttc ctcttttaga
     6061 actacacaaa tagaatcagg ggaatatacc ttaaacaata tgattggtat tatcctttcg
     6121 gaactgaatg ttatggacta taatccagta gggaataacg atccaataat ggttcctgta
     6181 atccaatcgg taagtgtgta tcccaatcct gcttctcaat cttcttctat cagctttaat
     6241 cttactaaat cagcaccgat ttctattgcc atctacaata ttaaaggaca aaaagtgaaa
     6301 gatttaatcg ggtcagaact gaaaagcggt aactatcaat ttacttggaa tggcaaagat
     6361 aataacggca ggtattgctc cagcggtgta tactattata gaattgttac cccagaaaag
     6421 aacatcacca aaaaaatgtt gatcttcaag taaagagggc gagatggtga gaaggttaaa
     6481 gagatagata gtaatattat ttcctaaagg aaaagtcttc tgtcatacct gctttcacag
     6541 caatggcaaa gtacattcta cctatgcaag aatgagatag tgtttattct tttgtaagga
     6601 agtaatagaa acctgaacat aagaatatta gatatgtaat tatctgtttc ttaatagatt
     6661 agtttatgac tctttaacac tatcttagct ctgttaaaga attattaaaa atttgttata
     6721 ggtagagaaa agcaggtatg aaaaaaggat taacaaagac gaaacggcaa aaaatgatta
     6781 catcatcttt ccagaaaatt agctggatgc agagagttaa tcattttgct ggggaaaaaa
     6841 tcataactta acacctttat aaactatgat attacgcaaa ataaagtgta ttctctggca
     6901 catttatctg ataagtgaat ttttccttgt ttctgccata attaggattt ttagtgctga
     6961 tcccgtatta cgcagaaaac ggctattgaa aaataacact cgcatcagca aacgctttat
     7021 tcgtgctctt aacattaaat taaccctaaa taactccgaa aatttacaaa aattgcagga
     7081 tattccttat ctggctgttt ccaatcatac aacttacctt gatattatat tgctctctgc
     7141 ggttgagaat tttgtcttta tcacctctgt ggaaatgaga aaaaatccct ttttgggtag
     7201 aataaccaaa agcggtggct gcctgtatac aaaccgaaaa aaatatatat ccttgcctac
     7261 tgaaattgag aaatttgctt ctgctattca tcagggcttt aaagttgttc ttttccctga
     7321 aggaacaagc accaatggca taacagttca gccctttcgc cggtcccttt ttcaagtggc
     7381 aatagaagca aaatgtccta tactgccagt ttgtattaaa tataaggcaa tagatggtaa
     7441 aaagatagat aaaaaaaatc gcgacctaat ttactggtat ggagatatgc ctttccttat
     7501 tcattatcta aaaattatag gacattccct tgaggcacaa atagatattt taacaattat
     7561 accctatcag gaagggaaaa ccagacaaga acttgctaat caggtctatg ggcaaattct
     7621 ttcctcctac ttggaacaag aaagttcagt tgacaaacaa agcaattctt tatctatggt
     7681 tcttaaatga aaaatcattg ctttctgcag cgaaggagca cagatgaaca ataaagacga
     7741 tgaaaaaaac aaactgcact taaaccgcac caattttatc ctgttgctgg tagcggcaat
     7801 tgtattagtt gttggctaca taattatgtc cttcaatgaa attactatat ccccaatttt
     7861 attggttatt gcttatgccg taatcgttcc ttttgccttg ctctatcacc ctaaaaagaa
     7921 ataagatgcc ccttttatcc gaagaagcat tagttcgcct gaataagttc cagctaactg
     7981 ctaaaagcat agttgagggc tttttagtgg gcttgcataa atccccctat catggtttca
     8041 gtgcagaatt ttctgatcac cgccaatata atcctggaga accgttaaaa gacctggact
     8101 ggaaaatttt ggcaaaaacg gaacgctact atgtaaaacg ctatgaagaa gaaaccaatc
     8161 tacgcagtta cattctctta gaccacagta aatcaatgtt cttcagttcc gggaaaagca
     8221 ctaaaattga atatgcaact caattggcag gtgctttagc gtggctgatg atttcccaaa
     8281 aggatgctgc tggactttat acatttaaca ataaaattac ttccgcctat ccccccaaag
     8341 ctattagaag ttatacttct cagttatttg ccgctctctt ggatttgaaa gcggaagacc
     8401 aaacagacct gttaactccc ttgcatcaaa ttgccgaact cgttaaaaaa cgctccctaa
     8461 taatcattat cagtgacctt ttggatgaac cggaaagaat tatggaggca tttaaacatt
     8521 tccgtgcccg aaaacacgaa gtactcgttt ttcacataat tgacccaaag gaagaaaagt
     8581 ttgattacaa aagagaagca caatttatag atagcgaaac aggagaaaaa ataactgtct
     8641 caccctggca aatacggaag gaatatgtag aaaattttca tcagttcgcg gaatatctaa
     8701 aaagggaatg ctacaaaaat caaatagaat acaatcccgt agatacctat actcctattg
     8761 aagacctttt gctgaagtat ctaattaaac gacagaaggg ttaaacaatg gcttacagat
     8821 atgtttcaca tgaaacaatt cggtctgaac ttgctaacgc cttaaaatat ataaccttta
     8881 accctgatcc tgaattaccc gccttgctga aaaatgccct tgccaatgaa acaagcgaaa
     8941 taagtaaaga tatccttaaa tgtatgttga aaaacataga attggcacct aaaatagaaa
     9001 ttcctttatg tcaagatacc ggttctttgg ttgtttttgc ggaaataggt aatcattgca
     9061 ttatagacgg acctccttta cctgaaatta ttaacgaaac cctaattata agctctgcag
     9121 aactctattt gcgtccgtca atacttaaag accccctttt gcaaagagaa aatacgggaa
     9181 ataatgcccc tgccgtaatt catatttcta tagtagaagg agataaacta aaacttcaga
     9241 ttgctcaaaa aggcggtggc gcagaaaata tgagccaatt aaagatgctt attccctctg
     9301 ccaaagctga tgagattaag aactttgtag tggagacagt tactctggct aaaggaaatg
     9361 cctgtccacc tttaatcatt ggaataggaa tcggaggcaa ctttgagacc tgtgctctgt
     9421 tagctaaaaa ggcattgttt caacctctga ccgctaaaaa tccaattgaa cgatatgccc
     9481 ttttagaaca agaaatcctg aaaggcgtta acgaaacagg aataggggtg caaggtttag
     9541 gaggaaaaac aactgcctta gctgtgcata ttttaaacgc tccctgtcat attgcctcct
     9601 taccggtagc agtaaattta caatgccatt cccaccggca tatagaaata gttatctaac
     9661 ttatgaaaac atatagattt accttgcctc tttgtcctgc tgatattgct caactgcata
     9721 tttctgataa ggttttgctt accggctgtc tgtttacagc cagagatgta gcccataaaa
     9781 gaatggtgga tattctgaaa agaggtgaaa aactaccttt aaacctggca gaaacaacac
     9841 ttttttactg cggaccaagc cctgccagac caggaaatat ttgtggagca ataggaccta
     9901 caaccagttc ccgaatggat atttataccc ctcttttaat tcaaaacggc ttaagagtta
     9961 tgattggcaa aggtgaacgc tctatagaag tccaaaaagc aattaaaaac ttcggagcga
    10021 tatattttgt tatgcctgga ggcatttctg cctatctttc acaacatatt gtttcctgtg
    10081 aaacatttct gtggcaagaa ctgggtcccg aagctattca taaactttgg gtgcaagata
    10141 taccggtgta tgtagcgata aaatagcgag atataaaagc cccaaagggc ttaacaatga
    10201 tagcgatgga tgcgtaagcc ccttgaaaaa agaaagaaat aattgtcaat atctcattct
    10261 ttcttgtgtt caaaaaaaca actaagggcg atacaataat gtagacggtt ttaaactcca
    10321 agacatcagg agctgcagca tagcaaaaaa agagattgac ataaatgggc attttctatg
    10381 cgtgggacag aaggtttaaa aaactgttaa gaaagagaaa caggagacca agcgtatgag
    10441 attattcaaa agtcgcctaa tctttctctt gctgtttctg ctcctattgt taagcacagt
    10501 gataattgca cagaagcagt cagacgcaga aagatatata gtagaggatt tcaaagtaga
    10561 agatattcct gcagatgacg gaacaggatt aatgctttcg tggaagccct tggagcggga
    10621 caaaagaatt attgaatacc gcatttacag gggagtaagt cctgataccc ttttcttttt
    10681 agatgccatt ccggttaatg taaaaacagg tgttgcttct gagaggatgt tctattatga
    10741 taattctggc agtgaatttt ttgatatttc ttcgccccgc aagttaaaga aagaaaagca
    10801 gcaggggaaa aatagtccct tatatcaaaa aatgcctcgt aacattcctt ttttagcaga
    10861 aatatccagt aaattttctc tgctttccgt tagcgaaaag aacaaatact attacagcag
    10921 tcagaaagtt acattggata aaccccaaga acaaggttcg gaaaaggaaa aacaggaaga
    10981 gggagaaacc tatgcgggat tgaaagacaa tcaacagaca gtgtattgtt tcttaaatcc
    11041 cggggagaac tactattata ccattgtagc ggtgaatgaa agaaggcagt ttcaaaagca
    11101 cgctaaaatt acagcaggta gccctaaacc taatgctcca gaaccggcaa cagcattaca
    11161 cagtgtggtt ttagaagata gcagggaatt acggtttgaa tgggaacttc cattatacaa
    11221 ttcggacata gctcaatatc gtattcatca aacacctctt tttacaccta cggagtggga
    11281 ttcgcttcgc ttacatcctg aattgtttca gaccaaaaca gtaattctgc aaagcggttt
    11341 agcaggttac ggttccggaa aaaattatag ccaggtttct attccagaag aggcgtctct
    11401 ggaggcatat attaattcag cttatacttt ggaattaata gatgcggatg gtttcagcag
    11461 tttttccgct ctttctaaac ccaggataac gcatagtaaa gaccttcctg ccaaaccgat
    11521 aataaaaatg gaagataagc ccaatgacaa aggtgaccgc ttaaccgttg tttgggatca
    11581 tcctatttgt tttgtagtta agaccacagc tctgaacagc aaattcaata aattcagggt
    11641 aaattatcag ctgaataaaa ccgaaactca gcaagtggat aacatctatt ttgagttcta
    11701 taagaagggt gaggaaaaac cctttgccaa aattaacgag ttttatcagg ataacagtgt
    11761 tatcttaaaa gttcccaata attacgatta caaaaaggga tttcgggtaa aaatcacttt
    11821 ggcaggtaag ccagaaatac ctaaggacta ttgtatagag caggatttgg aatatgaccc
    11881 caatatgctg gctattttac ctaccaaagc actttatcgt aatggcaaag atgtatctaa
    11941 aatcagcaat gtagtttacc gcaaaaatat ggcttccaag cagttaactt tggttaagcg
    12001 caatacctct tatgataatt ctttagatgt tacaattcct tatgctacta tgattcaaaa
    12061 gccggttaac ggttttactt ttgcccaggg tgatactttg ataacctata tcaatggaga
    12121 gaggcaagcg cgtaaattaa agccgggaga tataaaaacg cctttagctt taatttcgcc
    12181 caatattgat ttggtttatg ataaagaaaa tgaaacgcgc attgagacca atattttccc
    12241 cgatttaggc agaaaagaag cacaggaaaa ggtggaggag ctaacgaaaa aaattgcaga
    12301 gctaaaagac aagataaccg gtgagaccga tcctgctgta gttgaacagt taaatcaaaa
    12361 tatagagcaa ttacaaaagc aattaaatgc ttacagtaaa aatgaacatc tggttaatgc
    12421 caataaaatt acaaaccgga aaagcagaat gcgttatatt gctaaagtac gagaggacta
    12481 ttcccgatac agcacctatc aggttgtccg caccaatagc agagggcttt ttacggaaag
    12541 tgatctgtta aaagacggag atgatatatt ctatatgaag cccgtttcca atgtttttga
    12601 taccaataaa tatactaccc ttgtggcaat gcttattttc ggcttaatgg tagttatttt
    12661 tatcaattta gccaggcgtg gtaaggactt gtatataagg ccaattgccg gtttggaaga
    12721 aattgataca gcagttggca gagcaacgga aatgggacgt ccgattatgt atatgatggg
    12781 atatggttct ttaggagatg tagctactat tgcctcaatg ggaattttaa gtttggtagc
    12841 aaagaaagct gcagaatatg atattaaact tattgtaccc gtttataatt atatcgttat
    12901 gccggtagtt caggaaattg ttagagatgc acattattcc gtaggtcgtc cggatagtta
    12961 tgataaaaac agtgtattct ttttaaccga tgtccagttt gcttatgtag ctggtgtgaa
    13021 tggaattatg attcgggaaa gagctgcaac taatttctat atgggttatt ttgctgcaga
    13081 agcattattg atgactgaaa cgggtaatgg tattggtgct tatcaaatag ccggaactga
    13141 tgccatcacg cagattccct tttttataac aacctgtgac tatactttaa ttggagaaga
    13201 gctttatgcc gcttcttcct atttaaaccg tgaaccaatg caactgggaa ctttgaaagc
    13261 gcaggactat tacaaattcc tgatttttgc ctttgtaatt gctggagcgg ttctttccac
    13321 tttccagttg actggtctta gtgaattatt tcccctaaaa taaatgaggt tctaaatgaa
    13381 gaagacaata cctttaatca tcgtaatcgt ggggggcatc atctttgtgc tttatgcctt
    13441 aagtccccat aagcttttac aggatgactt ttataacaag ttctatcttc cctgggttta
    13501 tgcatcttct ccttgggctg ctcttttggg agttatcagt ttatgtatga tgcacagcag
    13561 taaaattaaa agaaaagctc ccaaatggca atacagttat ttcttttttg ccgggtttat
    13621 tattacttcc cttgctggtt ttatcggcgg aactcaaaaa ggtagtgttt tgatgtggat
    13681 ttttgagaat atgcagatgc caatgagttc cacaatgttt tcccttttgg cattttatat
    13741 ggcttcagct gcttataagg catttagagc gcgttccgtg gaagctaccg ttttgcttct
    13801 tgccgcaatt gtagttatgt tagcgcaggt gcctttggga gttaagattt ataagaatct
    13861 gccaattata tcacaatgga ttttggatgt ccctaatctt gcttccaaaa gaggaattgc
    13921 catcggtgtt ggacttggtg gtgtagcaac ttctctgaaa attcttttag gtattgagcg
    13981 ttcctatttg ggtggaacgg attaagcaaa gggaggatga agaatgaact tttttgaaag
    14041 aatgcaaagt ttagaccggc gctggatcta cattatagta gcgctggcaa ttatcatccc
    14101 gttaattata ccctacaatt cagataatgt aacttctccg cctacagaga acttgtatca
    14161 gatgattgat tcctttgccg gcagagaaga tagagctgtc cttttaagtt ttttacacga
    14221 tgctgctacg atgcctgaac tttatcctat ggaaatagct attatccggc attgttttga
    14281 aagaaatata aaggtttttg ctcttacttt tttaacttcg ggagctccta ttattgatta
    14341 tgctttcaat tcggtaaaag aagaatatcc tgatataaaa agcggggtgg actattgcaa
    14401 ttttggctat aaacctcagc caatggctgt agttttggga atgggagata atattgccaa
    14461 tgctgtaaat actgatgctg aagggcgtaa actggaaagc ttgccgatta tgaaaggaat
    14521 aaccaattac aacgaaatga acctggtagt agagttttcc ggtagcagtc caggagttta
    14581 ctggatatat tatgcccgac ccaaatttgg agttaatgtt gctttgggag ttacagcagt
    14641 tatggctgcc gatgaatatc cctatttgca atcgggtcaa ttgataggta tgctgacagg
    14701 acttaagggt gcagcggaat atgaaaagct ggttgatgtt tttgctgctt atcgtgatcc
    14761 ggcaattgac tatagtgtta aaacagatgc ggaaggaaac aagattctgc cagggcgtcc
    14821 ctttggcaag gaagttctaa atgatgagag cacaaagaaa ttgatcaata ttacaacgca
    14881 aacgaaggcg gaatttactc ccgaggaata tactgcattt gtagccaaat atccggagca
    14941 gaaagcaatt tttgaccaat tgaaagaaga acaaaacggc acaataatta ttgatgtaac
    15001 gaagataact ccggaactga gaaatcagat gggagaaact gcttaccggg aaatcaatcg
    15061 gcttacacat aatatcagtt ataaattcaa agtagccaga attggaatga atgctcaatc
    15121 ggttgcccat attatgatca tcatctttat ccttttgggt aatatcggtt atttcattca
    15181 gaaagccaaa acggcagaaa agtaagaaag ggaggataac aatgagtttt gaattattta
    15241 gtaatctcgt agcagcattt ttaaccctgt gtattttctc cttcctttat aaggaaaatc
    15301 ccttttatag aatggcggaa caactgttgg tgggagtttc cctgggttat actttagtgt
    15361 ttacttatga acggatattt attccgtatg tgtggcaacc gattattcta aaacataatt
    15421 ttgtccttat tttaccctgc ctgatgggaa tactttatct gttccgtttt tcgcgcaaac
    15481 tgggttggct ttcccgttat cctattgcct tttctatgat aggaatcggt tttggtgttc
    15541 cgatgggaat tcatgccgga attttagtgc agatgcgtca ggcgatggtt cccctggaaa
    15601 atattaatct cattcttatt cttttaggca caattgccgt tctgttatac ttctttttct
    15661 ccaaggcgca tactggtgct tacggaaaat ttgtcggatt gggaaaatgg tatatgatga
    15721 ttggttttgg tgcctccttt ggtttaacgg ttatggcgcg tatttccttg ctgattggaa
    15781 gaattcaatt cctcgttata gatgtttttg gattgatgaa atagctcaat ttaggaacct
    15841 tttagttccc ttttaacata aaagttgtat agattcaagt tactggtatt gtgggaagag
    15901 aaataaggga accttggttt agggatataa aaatgaaaaa atatcttatt gtaatcggaa
    15961 ttattctgtt gatagggatt tcattgcttt ccacggatga tgcaaaacct gcttccctga
    16021 aaggtaaaat aacagaattt tccgcttccg atatgccttt tgacgatggt agcggaattg
    16081 tattgaaatg gaaacctctg gataaatcgt atcgcattat taattataat atctatagag
    16141 gcgttagtcc cgattccctg ttttatattg gcagtataga agttgatccc aaattaggtg
    16201 ttttgtcacc ggaattatat ttctacgata agggagaaga accctttata gaatttgaaa
    16261 cggctcccca aaagctgaaa aaggaaaaac agcaaagcca taatagtccg cttttcaaaa
    16321 agtttcccca acagccggaa ttgctttcta caatgctgac cagatacaat attataggag
    16381 ccatcaataa ttccaagctt tatcatagtt ccaaaccggt gacaaaagat gaagatatta
    16441 tggcgggctt gaaattaaac cggtttgaaa ccattttagg ttttcccaaa gcaggccagg
    16501 aatattacta cacagttctg gcagtgaacg aaagaggaaa atatttgcct tatgcggata
    16561 ttcaaaaagt aattcctgaa gataatccgc cagaaccatc cgcaaatctt tccagcactt
    16621 acatccaaga tacgggagtt ttcaattttg aatggctacc accatcttct tcggaagata
    16681 ttgcaatgtg ggagggttgg ctgattcctt tagagcaaaa accaaataat ggtgaattac
    16741 tttctgaaaa ttggcaaaat agcgctattc cccttttcca gattcctaat tattccggag
    16801 ccgtaaatac ctgttatagt gtagatacca aagaagaagg gataaattta cctgccaatt
    16861 tggctgatta ttatcccgtg ctttcttaca gcgactatat gggtcaaagt gcagccgttt
    16921 cagctaaaaa ttttcgtatc ctgaattctt cacagcagct taaaataccc caaattgagg
    16981 ttaaagataa acccaacgac aaaggagatt atttatccgt ttctatggga aaacccctgg
    17041 tttttgtcag ccaagtgctt tatttgaata aggcaaagac agctttacgg ataaattacg
    17101 aaatttccaa taatgaacat tataaaatta aggaattgag gtttaccgtt cttgattcct
    17161 taaataaaga aattggcaag gtctccgaat actatttgga taaaacaatt catttcaaac
    17221 taccgcaaca gtatatcgct attaaaaata tgaaaattcg gattgccgtt caaactaacg
    17281 gcaacggtag ctttgaagat tatacagaac agcatattgt ttatgatgaa gggaataaaa
    17341 tttttcgggg tgagaatatc tactatcaga atgaaccggt gaataagctc tattatgaaa
    17401 ttcttacccg caatggtttt gatcctgatt ttttgtttgg agagaggact aatggcttaa
    17461 cccgtgctta tgaccattcc attccctacg aaagtattgt gtataaaagc attctggatt
    17521 atgataacaa gggtaagcgt ctgattttag acccgcaaat aacaatagct gttgatcagg
    17581 taaacggata tacttttgct gttcccttat tccgtaataa atttgaacaa gatctgaaaa
    17641 aacaaaaaga ggaactggat aatttattgg cggaaaaaaa caaatatcct gctaatgcta
    17701 ttcccgattc tttattggcg gcaattcagg aagcggaagg aaattataac tttataacga
    17761 agcatcctgc ttatctgcaa gctcaaaaga caaagaccaa taaggaatgg ctgaagacct
    17821 tattagctac gcataataaa aatatgcgca gttattccta caaggttatt aaaacagatg
    17881 gaaaagccaa ttttgtaact actgccattt atgctgatgc taaagggaat accacatttt
    17941 atccgcggag cgattggttt gatactacca aatatattac tcttgctgct tctattttat
    18001 tgtgtttatt ccttatttat gccatcattc aaagtcggaa aggaaatgct tatatcagac
    18061 ccattgccgg tttggaatct attgaagaag cagttggcag agctacagaa atggggcgtc
    18121 ctataatgtt tgttcccggt tggggaactt taggtgatcc cgatacggtt gcttcaatgt
    18181 tgattttagg gcaggtagca aaaaaagccg ccgaatttga tgtccgttta atttcacctc
    18241 attgtgacta tatggttttg cctttagccc aggaaattat tcaaaatgcg tataatgaag
    18301 ttggacgtcc ggatgccttt aattccaatg atattttctt catttcttat gatcagtttc
    18361 cttactgtgc aggaatcaat ggaataacag tgagggaaag agttgccacc attttttata
    18421 tgggatattt taatgctgaa gcactgcttt taacggaaac aggaaaccaa acaggcgctt
    18481 ttcagattgc cggaaccgat gctataaccc aagtgccgtt ctttattact acctgttctt
    18541 acaccttaat cggagaagaa ttttatgctg cttctgccta tatttcccgc aatcccgacc
    18601 ttgtcagtat gctgaaagca tcggactatt ttaaggcagt aattgttatt gttttgttga
    18661 ttggcacttt gctggcaacc ctgaatttta ccggtttcat caatgccttt ccgattgaat
    18721 aaaaaaggtt gagaggtctt aaacaccgga aggttgaaat ctccgtcaac ctctaataaa
    18781 atatgaattt accaatacaa tagttcatta ttctccggaa ttttcctaac tccttctaaa
    18841 taaatataat tacatcttct tccttgattg cattgcttac ctattgctta ctcattacta
    18901 atgccatccg taatgggtaa gcaatgcttc agcaattcag gcaggaaata aatcccattt
    18961 ttgtcaccga gaataacttt ttgtcacctt atattattat gggagcaaga taattggtct
    19021 accgaattct gctgtgcgat atgcggaaaa taatacaaac attataaata gcagatttaa
    19081 aaggcataaa tactttttat tttatgctga ttcctgcttg cacaggaatg accaataact
    19141 tatcctaaaa aattgatata aggagaaaag caatgaaagc aacacgcgcc aatcttctgg
    19201 gtgaatttac tggtcacttg gatggactga tctattaccg gaacaagaaa aacggcaaac
    19261 tttatgtgcg ccggcgcttt aaattcaaaa atcatcccgc tcatccggta tttacaaaaa
    19321 atatccaaaa catttatgca ctttcccctt cagagggtta taagcaaaac ttgaaaatat
    19381 atattgcctt gttcaacgca aatctgccgg aggggaaaaa gcccttgcac acttggacaa
    19441 atgcttacac aacattgatg ttcgctttgc aaaaggcgtt tcccgagacg gtaaatctcg
    19501 gcactcttac aagggaccaa atctatagcc aaaacctacc ctgcaaaagc gtgaaacagg
    19561 cagtagaagc aggtttgtta ccccttgtta agtcctatga actcctcagc gcagaatttt
    19621 agacaagata agctaaaacc ggggattttt gtggaggtgt tttgaaaata gcggactatt
    19681 cttgaagcca aataatacag ctactggaaa aagggttggc aaattctttc tttgc
//
LOCUS       DAOPFL010000025        19643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_2998_length_19643_cov_94.593373, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000025 DAOPFL010000000
VERSION     DAOPFL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 19643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19643
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2998_length_19643_cov_94.593373"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLG69_03215"
     CDS             complement(<1..977)
                     /locus_tag="PLG69_03215"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42550.1"
                     /translation="MKPNKISVFEYSRLKTGCEYNGVTFTPVLFESLKRFHSSSSIRY
                     FNLIHNGVEFCGYVGVIQVDGIQIEVLPKLDRNGGDANTWRDLLIDMLREVGMLRVSA
                     PSSSMLTLRSNSILELYFDLFITEVEYLIRTGLVKQYRRQTQNQTSLKGSIDFMRHLS
                     KNLIHKERFFTNTSVYDQDHIWHRIISQTISLIRLISQNTQIHNRIGALELNFPQVTS
                     QRISEKTFNKLVYNRKTENYRTAIEIARLLLLNFHPDLASGLNSVLALMFDMNVLWES
                     FIYHTLRKQFLINSAPYTVRAQHSTEFWSTESYRRYLRPDILIESVEDGR"
     gene            complement(967..>1626)
                     /locus_tag="PLG69_03220"
     CDS             complement(967..>1626)
                     /locus_tag="PLG69_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPI42551.1"
                     /translation="VLIIDEINRGNVSQILGELITLIEPDKRLGNEEELEVTLPYSKE
                     KFSVPSNLYIIGTMNTADRSVEALDTALRRRFSFEEMAPDYSLLEGSTSEGINLAELL
                     KTINNRIEGLLDKDHVIGHSYFLRVSKGEKTLQDVFFDEIIPLLQEYFYGNFGRIELV
                     LGRGFVASKPINQSIFAKPSPYNDEFNDHIHYSLVNRKKMNKIDFRKALQVLMNESNE
                     A"
     assembly_gap    1627..1726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1727..2735)
                     /locus_tag="PLG69_03225"
     CDS             complement(<1727..2735)
                     /locus_tag="PLG69_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42552.1"
                     /translation="MPFEPKKITRDNVLEAVKEIEENSLELIPSTQWDVIINGKAYPP
                     KDVLRYAHKQVNGELLWDISGGEATNTYLDEMGFLIRAKNDSANQIRAIIQKYKKTID
                     LSVFSNKDEKWYFVQRYKDNVNPENPNFVEAFGSINFANFVYPMPWAVIKDLLSKEPM
                     RYRQCIIKLFQDEVDLSQRIQTFMHEIEVIYHEMYPESKKSSYHDERTIATFLTLRYP
                     NNYTFYMEKLYRKLCSLMGVKPMSREGEKYVHYLKYMHEFTSQYVIADQELISMVSQA
                     IPSDAYPDNDHLLLSQDIIFRLSGKDSTLDIAYPLNQILYGPPGTGKTYNAIKKAVAI
                     AD"
     gene            4153..4611
                     /locus_tag="PLG69_03230"
     CDS             4153..4611
                     /locus_tag="PLG69_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPI42553.1"
                     /translation="MEEYAVIFNQYLSNKGLKLTKPRRCILETVFNMHTHFNVEELYD
                     KIRAKTNAISLATVYRTIPLLLDAGLVQHALRSEGRERYEHIFGHPKHIHWLCRNCGA
                     LMETDLNTLYPVLEKQAQDLKFKPEEIELNIKGLCWKCNPIENETQSDEK"
     gene            4586..6781
                     /gene="feoB"
                     /locus_tag="PLG69_03235"
     CDS             4586..6781
                     /gene="feoB"
                     /locus_tag="PLG69_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPI42554.1"
                     /translation="MKLNPMRNRPVIALAGNPNVGKTCLFNALTGSHQTVGNWPGVTV
                     EHKSGRCNYKGKIYEVVDLPGIYSLAGGSPDELVARNYILTEKPDIIINIVDASNLER
                     NLYLTVQLMELGAPLIICLSMIDIAEHNGIYIDTEHLSSHLGFAVFPLVLNKRIEVSP
                     ILDKVEEYLVQPPVPATIHYDEVVEKHLQNIWQIVLKSGLGEPETKVPQRKFSESASI
                     AYDILIKSRWQALKLIEGDAELTSRLSDEDRALVEKEISAIAKHRGQEPTFVIADDRY
                     GYIRGLIKDVVKRKRKSRLTFSDMVDKVVLNSVVGLPVFFIVMYLVFLVAVKLSQPCI
                     KFIEFILSWLFIEQLGNLLSSPSFPQWLNYLLSKAIGGGIVTIGSFIPPIFFIYISLS
                     ILEDSGYMARAAFIADKFMRKIGLPGKAFIPLLVGFGCTVPAIMATRTLEKPRDRVFA
                     SLLTPFVSCGAKLPVYTFLVMLFFPQYADLLIFGLYLFGIIMGVLTGLLLKKTIFLSA
                     PGDFVMELPAYHIPTINGIFMHTWHRLKSFLLRAGKTILLVIVLINILQGIQIPTGRD
                     SEKTSILELGGKLITPVLQPMGIKRDNWQSSVALISGLFAKEAIVGTLQGLYQNSEGE
                     ISADQLANNIKSGFGSTASILAYLVFILLYSPCAASLTILFKEHGFKWMLFAFVYLTL
                     IAWMVATLIYQILAFNVLSIFWFVVIGAIFTFIYITLHKIGSKYAFQTE"
     gene            6762..7064
                     /locus_tag="PLG69_03240"
     CDS             6762..7064
                     /locus_tag="PLG69_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPI42555.1"
                     /translation="MLSRRNRVRIRGWSRKFSGRKRHGVCKAGQCLPLDQLPEGCNAV
                     VIHNNNLKIIERGLYIGAQISMFRNDDDEPNVVVAVGDARYVLDRRIAKTIKVKIV"
     gene            complement(7073..8590)
                     /locus_tag="PLG69_03245"
     CDS             complement(7073..8590)
                     /locus_tag="PLG69_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPI42556.1"
                     /translation="MKKDSFNTEIIVNVHPLEKRVAVLEDSRLVELFVERREQQNIVG
                     NIYKGIVKDVLPGMGAAFIEIGLERTAFLHYSDIVMDFLDIYENALPRTKVNPEDSSQ
                     IDKLLKPGQEIVVQVHKGPIGSKGARLTGQISIPGKYLVLFPNKDKIAVSRKIYSQAE
                     RSRIRNLLSGIKDPGYGLIVRTEAEGCEEDDFQTEYKALIKTWHLIEKQIKYAKAPVC
                     VFEENALENYLIRDLFGENVDRLVIDDKNFAKRIISQLTDISPELINNIEIYREDSPI
                     FDAWGIEKKIETVLHSRIYLPSGGNIKIEQTEALVAIDVNTGSFTGKTNYDETVRKTN
                     LEAAAEIARQIRLRDLSGVIIIDFIDMVEEKYKNEVLEMLKKGLRRDRAKNKVYSFTE
                     LGLVEVTRKRMRNTLISNFSDPCPFCNGSGRILSKDSVIMRIYRWLNRSDYFIKDKNL
                     RIVVNPDLLNHIKQHNEDFIAYKEQIEFASDPSIRIDQFKIYLLPGLEDITSKFS"
     gene            complement(8663..10381)
                     /gene="sppA"
                     /locus_tag="PLG69_03250"
     CDS             complement(8663..10381)
                     /gene="sppA"
                     /locus_tag="PLG69_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPI42557.1"
                     /translation="MKSNKTVLWGCLIFVIALLAAFILGFIFTFGSHGAKSAKIPSNA
                     WLYVNPSALLPDYNELEELRFVNVSSPSVQEICAKIKLAAKDERIKGILLQPQMIMTN
                     YPALEEMSLAIGTFQKSGKPVLAFGENFTQGDYLLASCADKIYMEPSASAGLALQGVS
                     ANMLFYKEMLDKLGIKMHILQSGEYKGAGEPFSQTELSKGTKENIDAALYDIYNHLLS
                     LVAQNRKLETTQVKDIFEKRDDFFLSAEKAKELKLIDYAMSRDEMLKSLDLEEDNFVK
                     IANYSPTAKKKKGDKIAVVYLNGNIAPVSRSNFGSQGIISEAKVKKIIKQIHQHKDIK
                     AVVLRINSPGGSALESELIYQQLLKLKREYPLVVSMGGTAASGGYYISCAGDYLIADP
                     GTLTGSIGVIGLIPEMAGLGKKIGVRSQTLKYGKFAGALSPLEHYDPALIESLKRSST
                     ATYNEFKQRVMTARKISPENIEAVAEGRIFSAEDALANKLIDEIGTLDKAIAKAAGLA
                     KVTSYSSVNFPKKESYWEAIKESDLLNLKERLQNNNDPATQLEKYLKQISATGEWLYL
                     MPYTLE"
     gene            complement(10405..10536)
                     /locus_tag="PLG69_03255"
     CDS             complement(10405..10536)
                     /locus_tag="PLG69_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42558.1"
                     /translation="MIFFNLKDINTPAGKQVAKFQFAKALKVIYNISVMRIISSGIR"
     gene            complement(10543..10633)
                     /locus_tag="PLG69_03260"
     tRNA            complement(10543..10633)
                     /locus_tag="PLG69_03260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10596..10598),aa:Ser,seq:gga)
     gene            complement(10825..11985)
                     /locus_tag="PLG69_03265"
     CDS             complement(10825..11985)
                     /locus_tag="PLG69_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42559.1"
                     /translation="MKSNLTGIIKFIESKNAIKDKNKLTKLVSRRFKLIRDRSVYYND
                     EFAIRFCKSARPNLSNTILSLSNLRKVDDRPFIVCVVTPDKNYMYLANSTFLSKISHS
                     SQLLRTNNIKGSFNGSDIRKEFNGYKNEPDNFAVLFDFHKQFSFEENLARLVEATNDI
                     HPSGKKLELKDSEIKQILAAPLRAQSFVNSNEYIILKEELDARVKQYRNEILIASLIE
                     NSNIRSAVIEYLIAGNDDSLRKEIIKSLNSGKGYQSLTSNKHSLGDYSREFANFLTET
                     DVKTKIMVLDSNPKAYNIDKILEFLTKERTVFMFYFVGIDYLKNSVKTTLISMFQKDL
                     CNSIFILSHWSGRNSRGVTQFDGKVIKKLILNPDNSIDIKKAQDLLKKLIEL"
     gene            complement(11957..13084)
                     /locus_tag="PLG69_03270"
     CDS             complement(11957..13084)
                     /locus_tag="PLG69_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPI42560.1"
                     /translation="MVSKNIHNYEIDFAPTKDEKSEKNISDLIPLSNNKIGGEDKMKH
                     TAPRNRTLTVSEEELKKMKTRFINLNKQCMLDAIKGKVICQDALECIQFLPEKSIDLL
                     IIDPPYNMYKKFNEVTFNKKTIDEYAEWIDSWFSQIVSKISLTGSVYVCCDWQSSSAI
                     ETVLRKHLIVRNRITWERDKGRGAKTNWKNCSEDIWFATKSNKFVFNVDKVKIMRKVI
                     APYRDENGDPKDWKETNTGNYRLSHPSNFWTDITIPFWSMPENTDHPTQKPEKLLAKL
                     ILASSNEGDFVFDPFCGSGSTLVVAKKLNREFSGIEIDEYYCALTLKRLDLADIDKSI
                     QGYSEGLFWERNSLTEQQKIKLSSRKQKGIDNYEIEFDWHH"
     gene            complement(13202..14749)
                     /gene="purH"
                     /locus_tag="PLG69_03275"
     CDS             complement(13202..14749)
                     /gene="purH"
                     /locus_tag="PLG69_03275"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPI42561.1"
                     /translation="MNKYALISVSDKTGIEYLAVELEKMGYNILSTSHTADYLKQFCQ
                     KVVLVSDLTGFPEILEGRVKTLHPVIYAGILADRNNPAHAKTLSELKIEHIDVIAVNL
                     YPFASVCWKENATEQEIIENIDIGGPALIRAGAKNYKSVTVLVDPVDYAPALELLKQN
                     SILPEKFSSYLAQKAFAKTRDYDAEIAAYFASQGIEESIANELPAQLEFAISLKRKLR
                     YGENPHQNGGFYAKVTSGWELIHGKELSFNNIMDIDSAFRAIRLFGKPTAIIIKHCNP
                     CGIGSDETLAEAYRKAYETDTEAPFGGIVIVNRPLDLETATLINNIFTEIIIAPAFEP
                     GVLEFLKKKKNRRLIHYEFSLLEKPLNPIEIKTLTWGYLAQDWDLVNESTENWKIVTN
                     KQPSPEELEALIYAWKAVSVLKSNAIAIVKKDRVLGLGCGQTSRIDAVQLALWKAKKF
                     GHDLTDSVCASDGFFPFRDSIDTLAKNGISAIIQPGGSKNDEECISACNELNIAMVFT
                     GVRHFKH"
     gene            complement(14746..15825)
                     /locus_tag="PLG69_03280"
     CDS             complement(14746..15825)
                     /locus_tag="PLG69_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HPI42562.1"
                     /translation="MSKKKQNKGVFQKLLLWLYSYIKIVISDLKKIYQSITVPQQRKK
                     VFGNIWTFIKTFYQRFMGEGILKESASLTYITLLGFVPFINFLVLIAPDLPFLNLGDK
                     LRELVVQNFIPSSANAIINFLDKLIMPKVGFNIFNFVILIVSSYSLFSVIRGTFDRIL
                     SMQLKSSQDTITQLVKFFGTIVFGLLIIVLLFSSSSIPIISRLLKSPVLQWIMVIVPF
                     ILQFLAIMFLYMLLPSVRINRASLFRGAFWTTVIWVIGKSAFDFYIYNLTNYERLYGV
                     MAVLPIFLLWIYINWAIILGGMVLVSEMENKKNGGFLTKEPHNAVRITMELFTNQKLN
                     QRLEGILSKEELKKLTDTLNEEDDK"
     gene            complement(15825..17060)
                     /locus_tag="PLG69_03285"
     CDS             complement(15825..17060)
                     /locus_tag="PLG69_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI42563.1"
                     /translation="MAIPIAESLKLGFADIMMRKVRSIVTVIGIILGVMCIMVVLAIV
                     NGMNESTMSWMEERGGLNKIEVEQNWFYDFSKGGDPSFSLKEIRYLQSLIPEAEAFNP
                     TVQEWEAVINKGDIRYSTSLRGVMPDFVKVEQWDIAKGRFIKDLDIDQHSNVIVIGST
                     VAKEFFGNNDPLGQIISVSNQQFIVVGVMAERFMQAQGAQLGMGENALEYLNRRCFIP
                     ISTMISKVNPGSKISSIDIRAKSPEEAKELRRKVENIVLNLKNGKHLFQVTSAKEQLD
                     TMQANAKIFEAIFVLIAVISLLVGGIVIMNIMLASIRERTREIGVRIAVGARRRDIFI
                     QFLVQTVLITALGGILGILLGFAILDKVGSYLQIKVLASVQMIWVALLVSIGVGLIFG
                     VGPAVRAARLDPVIALREE"
     gene            complement(17041..17724)
                     /locus_tag="PLG69_03290"
     CDS             complement(17041..17724)
                     /locus_tag="PLG69_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42564.1"
                     /translation="MDIIKTENIVKDYILGKIKVRALNGIDLQIQKGEFVAIMGPSGS
                     GKSTLMHILGCLDSPTDGTYYLDDVLVSKMPKASLAAVRNRKIGFVFQSFNLLPHLNI
                     LKNVELPLMYGGMSKRKRTAKAKEVLQNVGLGDRLKHKPGELSGGQRQRVAIARAIVN
                     EPSILLADEPTGNLDSQSGGDILEIFTELHSQGNTVIIVTHDQAIASRAERIIKIKDG
                     KIVDGNSNS"
     gene            18330..18758
                     /gene="rplM"
                     /locus_tag="PLG69_03295"
     CDS             18330..18758
                     /gene="rplM"
                     /locus_tag="PLG69_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPI42565.1"
                     /translation="MKTLTPSPSDIHQQWYIVDAEGKPLGRLSTKVATILCGKHKPYY
                     VPNIDTGDYVIVINAAKVRVTGMKALQKVYKSFSGYPDGLKEIPYAKVLEEHPERIIE
                     HAVKGMMPKNKLGRAMFKKLKVYPGAEHPHSAQKPIELNI"
     gene            18771..19157
                     /gene="rpsI"
                     /locus_tag="PLG69_03300"
     CDS             18771..19157
                     /gene="rpsI"
                     /locus_tag="PLG69_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPI42566.1"
                     /translation="MQTFDAVGRRKNAVARVRLMPGTGKRIINNIQMKKYLQRETLEM
                     IVEQPLQTVGLSDNFDVYVNVYGGGLSGQAGAIRHGISRALVEYDENLRPVLKTRGFL
                     TRDPRMVERKKSGRPKARKRFQFSKR"
     gene            19397..>19643
                     /gene="rpsB"
                     /locus_tag="PLG69_03305"
     CDS             19397..>19643
                     /gene="rpsB"
                     /locus_tag="PLG69_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPI42567.1"
                     /translation="MSVVTMKQLLEAGVHFGHQTFKWNPKMKKYIFIKRNGIHIIDLK
                     QTVDAINEAYQFMKEVASKGEYILFVGTKKQAQAAIKE"
BASE COUNT         5809 a         3839 c         3565 g         6330 t
ORIGIN      
        1 cgtccatcct caacgctttc tatcaagata tcaggccgca gataccttct gtatgattct
       61 gtagaccaaa actcagtgga gtgttgcgct cttacagtgt aaggagcaga gttgatcaag
      121 aactgctttc tcagggtatg atagataaaa ctttcccaga gcacattcat atcaaacatc
      181 agggctaaaa ccgaatttaa gccggaagcc agatcaggat gaaaattcag aagcaatagc
      241 ctggcgatct ctatcgcggt gcggtaattt tctgtctttc tattgtaaac aagcttgttg
      301 aatgttttct cggatatcct ttggctggtc acctgtggga aattgagttc caaagctcca
      361 attcggttat ggatctgagt attttgggag atcagtcgga tcaaactgat ggtttggctg
      421 attatcctat gccaaatatg atcttggtcg tatacagatg tattagtgaa gaacctttct
      481 ttgtgtatca gattttttga gagatgccgc ataaagtcaa ttgaaccctt gagcgatgtt
      541 tggttctgag tttgcctgcg gtattgcttc accaggccgg tgcggattaa atattccact
      601 tcagttatga atagatcgaa atagagctca agaatagagt ttgacctgag ggtcagcata
      661 cttgaactgg gagcggaaac ccgtaacata cccacttccc ttaacatatc aatgagaaga
      721 tcacgccagg tattagcatc accaccatta cgatccagtt tgggtaatac ctcaatctgg
      781 attccatcca cctggattac cccaacatat ccgcagaatt caactccgtt atggatcagg
      841 ttaaaatacc gaatactact ggaggagtgg aagcgtttca acgactcaaa tagcaccgga
      901 gtaaaagtaa ctccattgta ttcacagccg gtcttaagcc ggctatattc aaacacgctt
      961 atcttgttag gcttcattac tctcgttcat caacacctgc aacgctttcc ggaagtcgat
     1021 cttattcatt ttcttccgat taacaaggga atagtgtatg tggtcattga actcgtcatt
     1081 atagggggat ggcttagcaa aaattgattg gttgatgggt ttggaagcaa caaaacctct
     1141 gcctaacaca agctctatcc tgccgaaatt gccatagaaa tactcctgca ggagggggat
     1201 aatctcgtcg aaaaacacat cctgaagggt tttctcacct ttggacactc tcaggaaata
     1261 actgtgtccg atgacatgat ctttatccaa caatccttct attctattgt tgatcgtctt
     1321 gagtaattct gccagattta ttccttcgga agtactgcct tcaagcaatg aataatccgg
     1381 agccatttct tcgaaactga atctacgtct gagagcagta tccagcgcct ccacacttcg
     1441 gtctgctgta ttcatagtgc caatgatgta aagatttgaa ggcacggaga atttctcttt
     1501 gctatacggt aaggtgactt caagctcttc ttcatttccc aaccttttat cgggctcaat
     1561 aagagtgatt aactcgccaa gtatctgaga gacattgcct cggttgatct cgtcaatgat
     1621 gaggacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatc tgcaatagct
     1741 acggcttttt tgatcgcgtt gtatgtcttt cccgtgccag gcgggccata gagaatttgg
     1801 tttaaaggat aagcaatatc cagggtcgaa tccttaccag acaatctgaa gattatatct
     1861 tgtgatagaa gcaagtgatc attatcagga tacgcatctg aggggatagc ctggctaacc
     1921 atagaaatta gctcttgatc agcaataaca tactgtgagg taaactcatg catgtatttg
     1981 agataatgga catacttttc cccttctctg gacataggtt ttactcccat caaagaacat
     2041 aatttcctgt acaacttttc catataaaaa gtgtaattat tcggataccg caatgttaga
     2101 aaggtggcta ttgtcctttc atcgtgataa gaactctttt tactttcagg atacatttca
     2161 tgataaatga cttcaatttc atgcataaat gtttgtatcc tttgagatag atcaacttca
     2221 tcttgaaaca gttttatgat acattgccgg tagcgcattg gctctttaga taggaggtcc
     2281 ttaatcacag cccaaggcat aggatagaca aagttggcaa aattgatact accaaatgct
     2341 tcaacaaaat tcggattttc agggttaaca ttgtctttat atctttgtac aaaataccac
     2401 ttctcatctt tattggaaaa cactgacaaa tcaatggttt tcttgtattt ttggatgatt
     2461 gcccgtattt ggtttgcact gtcgtttttg gctctgatta gaaaacccat ttcgtccaag
     2521 tatgtatttg tggcttctcc accactaata tcccataaca attctccgtt aacttgctta
     2581 tgtgcatagc gcaatacatc cttgggtgga tatgctttcc catttattat cacatcccac
     2641 tgtgtcgaag gaatcaattc taagctgttt tcctcaattt ctttaacagc ttcaagaaca
     2701 ttatcacgag ttatcttttt aggttcaaat ggcatgcttt ctcctataca taggtctctg
     2761 tggtctaata aaattccaga aacctcttta atttgacatc ttgaagacct tcccaatagt
     2821 gttgagatcc aaatgttggt gtaaattcca actctttgtt tctcagttta tttgaaggca
     2881 tctaataacc ctcgtttctc ataatctatg taagggcaaa taaccaatga ctttcttttt
     2941 tatttattta atccttgtca agtttttttt aaaataagct atcttgctgc gacgaacccg
     3001 tcgcgggatg tctcaaacag tataggactt atcatttttt cccggatcct ttccaatggc
     3061 tctggaagat acaatttacc gcttagccaa tccctctgaa tattcttgta gtatagaacc
     3121 gaacagcagc aagtaactca tttcattcgg gaagattctg atccatttct ctttctgcct
     3181 gagctcttca tttaggcgtt ccagtgagtt ggtacatttt atctttctcc agtgatctgt
     3241 tgagaaatta agataaacaa atatctcata gtaagtagca tccagccaag gcaaaagttt
     3301 atctttgtct tccgtctgga caacccggtt aaactctctt caagccaact caaacgactc
     3361 cttggttctg gaacctacca actcttttag ttttatttaa accatctaaa caaagagcgg
     3421 gatatggctc aaccttgttc tgcatccggc ttaaccaatt ttacaccaac ctttaagaca
     3481 caacctattt acagggtaag ttactaatct tatagcctat aatgaagagg aagaaggtga
     3541 ttatacagat gaagaatttg ctgaattatc ttccttcgtt attggctacc aactcaatct
     3601 gggtctggat ttcaataaaa ttctttgtga attcagatat gaaaggggtc tcactcccgt
     3661 atttgataat agttatatag acgagacatt ttatagcaat ttcaaatttc ttgttggctt
     3721 ccgtttttaa agtaatatag acggaggaca atgttcctca tgaagttttt tcacgcagat
     3781 tacgcagatt ttgtgtaatt cgcggatttt taattttccg aggtgcttac gcaccctcta
     3841 catcattgtg tcgccctccg ggctttttta aaaaggaaag ggaaaagaaa aaactcgggg
     3901 ctttcgtttt tacgaggggc ttacgcaccc tctacatcgt tgtttcgccc tccgggcttt
     3961 taatgtagct caaccctcta aactctctaa actctctcaa ccctctcaac cttctaaacc
     4021 ttctcaacct tctcaactct ctcaaccctt tcaaccttct caaccctctc aaccctctca
     4081 accttttttc ttgacataat tcgccagtta aatttagttg caaatgatta tcaatacaaa
     4141 gaggttaaaa agatggaaga atatgctgtt atcttcaatc aataccttag caataaggga
     4201 ctcaaactta ccaaacccag gcgttgtatt ctggaaacag tattcaatat gcacacccat
     4261 tttaatgtgg aggaattgta tgacaaaatt cgggctaaga ccaatgccat ttctctggca
     4321 accgtttatc ggactattcc cttactattg gatgcagggc ttgttcaaca tgccttacgc
     4381 agtgaaggca gagaacgcta tgaacatatt tttggtcatc cgaaacacat tcattggctg
     4441 tgcagaaatt gtggtgcctt aatggaaacg gatttaaata ctctttatcc ggttctggaa
     4501 aaacaggcgc aggacttaaa atttaagccg gaagaaattg aattaaatat taaagggctt
     4561 tgctggaaat gcaatccaat tgagaatgaa actcaatccg atgagaaata gacctgtaat
     4621 tgccttagcg ggcaatccca atgtaggtaa gacctgcctt tttaatgcgt taaccggttc
     4681 acatcaaaca gttggcaatt ggcctggagt aacggttgaa cataaatccg gaagatgcaa
     4741 ttataaaggc aaaatctatg aagttgttga tcttccaggc atttattctt tggcaggagg
     4801 aagtcctgat gaattggtag cccgcaacta tattttaaca gaaaaacccg atattatcat
     4861 caatattgta gatgcttcca acctggaacg caacctgtat ttaacagtgc aattgatgga
     4921 attgggagct cctcttatta tttgtctttc aatgatagat atagcggagc acaatggaat
     4981 ttatattgat actgaacatt taagttctca tcttggtttt gcggtttttc ctctggtttt
     5041 gaataagcgg attgaggttt ctcctattct ggacaaagtg gaggagtatt tagttcaacc
     5101 acctgttccg gcaacgattc attatgatga agtagtggaa aagcacttgc aaaatatttg
     5161 gcagattgtt ttgaaatcag gtttaggaga gccggaaact aaagttcccc agcgtaaatt
     5221 ttccgaatcc gcttcaattg cctatgatat tttgataaaa agtcgctggc aggcattgaa
     5281 attgattgaa ggtgatgcgg aattaacttc ccgtttatcg gatgaagata gggcacttgt
     5341 agagaaagag atatctgcta tagcgaaaca tagaggtcaa gaacctacct ttgtaattgc
     5401 tgatgatcgt tatggttata ttcgcggttt aataaaagat gtagtgaaac gcaagcgtaa
     5461 aagccggtta actttttccg atatggtaga taaagtggtg ctgaatagcg ttgtaggatt
     5521 gcctgtcttt ttcattgtaa tgtatttggt ttttctggtt gcggtaaaac tcagccaacc
     5581 ctgcatcaaa ttcatagaat ttatactcag ctggttgttt atagagcaat tgggcaattt
     5641 gttaagttca ccttcttttc cgcaatggct gaattattta ttaagtaaag caatcggggg
     5701 aggaattgta accattggca gttttattcc tcctatcttt ttcatataca taagtttatc
     5761 cattttggaa gattccggtt atatggcaag agcagctttt atagcggata aatttatgcg
     5821 gaaaataggg cttccgggta aggcatttat acctttatta gtaggttttg gttgcacagt
     5881 tccggcaatt atggctacgc ggactttgga aaaacctcgg gacagagttt ttgcctccct
     5941 attaactcct tttgtatctt gcggagccaa gcttccggtg tatacttttc tggtaatgtt
     6001 atttttccct cagtatgcgg atttgttgat ttttggtcta tatctttttg gcataattat
     6061 gggtgtttta accggtttgc tcttaaaaaa gaccattttt ctgagtgctc cgggtgattt
     6121 tgtaatggaa cttcctgcct atcatattcc tactataaac ggtattttta tgcatacctg
     6181 gcatcgcttg aaaagttttc tgctgagagc cggtaaaacc attttactgg taattgtatt
     6241 gataaatatt ctgcaaggta ttcaaattcc aacaggaagg gatagcgaaa agacctcaat
     6301 tctggaatta ggaggaaaat tgattactcc tgttttacag ccgatgggaa ttaaaagaga
     6361 taactggcag tcatccgtag ctttaatcag cggacttttt gccaaggaag caattgttgg
     6421 aaccttgcag ggtctctacc aaaattcaga gggagaaatt tcagctgatc agttggcaaa
     6481 taatatcaaa agcggttttg gcagtactgc ctctattttg gcttatcttg tttttatctt
     6541 gctctattca ccctgcgctg cctcgctcac aattcttttt aaggaacacg gctttaaatg
     6601 gatgcttttt gcatttgttt atttaacttt gattgcttgg atggtggcta ccctgatata
     6661 ccaaatactg gcttttaatg ttctgtccat cttctggttt gttgttatag gagctatttt
     6721 taccttcatt tacattactt tacataaaat agggagtaaa tatgctttcc agacggaata
     6781 gagtaagaat tcggggttgg agcagaaaat tttccggcag aaaaagacac ggtgtatgta
     6841 aagcaggtca atgcctccct ctggatcaat tgccggaggg ctgtaatgct gtagttattc
     6901 ataataataa cctgaaaata attgaaaggg gcttatatat tggagcgcaa atttctatgt
     6961 tccgcaacga tgatgatgaa ccgaatgtag ttgtggctgt tggcgatgcc agatatgttt
     7021 tggatagaag aattgccaaa actataaaag tgaaaattgt gtgaaaagtg cactatgaaa
     7081 acttggatgt tatatcttcc agtccgggaa gcagatatat cttaaactga tctattctaa
     7141 tggaaggatc ggaagcgaat tcaatctgtt ctttgtatgc aataaagtcc tcgttatgct
     7201 gtttgatatg gtttaacaaa tcgggattaa ctacaatgcg taagttctta tctttaatga
     7261 aataatcgct gcggttcagc caccggtaaa tacgcataat aacagagtcc ttgcttaaaa
     7321 tgcgtccgct tccgttacaa aaaggacagg gatcagaaaa attggagatt aaggtattgc
     7381 gcattctttt acgggtaact tccacaagtc caagctctgt aaaagaatag accttatttt
     7441 tggcacggtc tcggcgtaaa ccttttttca gcatttccag cacttcgttt ttatattttt
     7501 cctcaaccat atcaataaaa tcaatgataa tcactccgga aaggtcacgt aaacgaattt
     7561 ggcgggcaat ttctgcggct gcttccagat tggttttgcg cactgtttca tcataattgg
     7621 tttttccggt gaaacttccg gtattaacat caatggcaac cagggcttct gtttgttcaa
     7681 ttttgatatt tcctccgctg ggaagataaa tacggctatg cagaacggtt tcaattttct
     7741 tttcaattcc ccaggcatca aaaataggag aatcctctct gtaaatttct atattattta
     7801 tcagttcagg cgaaatatct gtcagttggg aaataatgcg tttggcaaag ttcttgtcgt
     7861 caataaccag gcggtctaca ttttccccaa agagatcacg aatcagatag ttctccagag
     7921 cattttcctc aaacacgcaa acaggtgctt tggcatattt aatttgtttt tcaatcaaat
     7981 gccaggtctt tatcaatgcc ttatactctg tttgaaaatc atcctcttca cagccctccg
     8041 cttcggttct aacaattaaa ccgtatcctg ggtctttaat tccggaaaga agattacgaa
     8101 tgcgactgcg ttctgcctga gaataaatct tacgggagac ggcaatttta tctttattgg
     8161 gaaaaagcac caggtatttt cctggaattg agatttgacc tgttaaacgt gcacctttgg
     8221 aaccaatggg tcctttgtgc acttgaacca caatttcctg acccggtttt aagagtttat
     8281 ctatttgaga ggagtcctca ggatttactt tcgtgcgcgg tagggcattt tcataaatat
     8341 ccaggaaatc cataacaatg tcactgtaat gtaaaaaagc ggttctttcc agtccgattt
     8401 caataaaagc agcacccatt ccgggtaaaa catcctttac aataccttta tagatattgc
     8461 caactatgtt ttgttgttct ctgcgctcca caaagagctc tactaaacgg ctatcttcca
     8521 aaaccgctac ccgcttttct aaggggtgga catttacaat tatttctgta ttaaatgagt
     8581 cctttttcat tttagtccaa attatatctt agatagttat gtctggttga tgagaacatc
     8641 aaccatcctg ctgtttttta ttttactcca gggtataagg cattaaatat agccattcac
     8701 ctgtggcaga tatctgtttc agatattttt ccagttgtgt tgcaggatca ttgttatttt
     8761 gtaaccgttc tttcaaattc aagaggtccg attcttttat tgcttcccaa taactttcct
     8821 ttttagggaa atttacagag gaatatgaag ttacttttgc cagacctgca gctttggcaa
     8881 ttgctttatc caaagttccg atttcatcaa taagtttatt ggcaagagca tcttcggcac
     8941 tgaaaatgcg tccttcggca acggcttcaa tattttcagg ggatattttg cgagcagtca
     9001 tcaccctttg cttaaattcg ttgtaggtag ctgtggaact gcgtttcagg gattctatca
     9061 gagccggatc atagtgttcc aaaggactta aagcaccggc aaatttgcca tatttcaagg
     9121 tctgagaacg cactccgatt tttttaccca accctgccat ttcaggaatt aaacctatta
     9181 ctccaattga accagttaaa gttcccgggt ctgctattaa ataatcccca gcacaggaaa
     9241 tataatagcc acccgaagca gctgttcccc ccattgaaac aaccagagga tattctcttt
     9301 ttaatttaag caattgctga taaataagct cggattccaa agcggaacca ccgggactat
     9361 ttattcttaa aacaaccgct ttaatgtctt tatgctggtg aatctgcttg ataatcttct
     9421 tcacttttgc ttcactgata atgccttgac tgccaaaatt ggaacgcgaa acgggagcaa
     9481 tatttccatt cagataaaca acagctattt tgtcgccttt cttctttttg gcggttggag
     9541 aataattggc aattttcaca aaattatcct cttccaagtc caaacttttc agcatttcgt
     9601 cacggctcat agcgtaatca atcaatttca gttcttttgc cttttcagcg cttaaaaaga
     9661 agtcgtccct cttttcaaaa atatctttta cctgggttgt ctccaacttt ctgttttgtg
     9721 caacaaggga aagtaaatgg ttataaatat catacagagc ggcatctatg ttttcttttg
     9781 tgcctttact aagctcggtt tgcgaaaagg gctcaccggc acctttatat tcaccggatt
     9841 gtaaaatgtg cattttgatg cctaatttat ccagcatctc tttgtagaat agcatattag
     9901 cggaaacacc ttgtaaagct aaacctgccg aagcagaggg ttccatataa attttatcag
     9961 cacaggaagc aagtaaatag tccccctgcg tgaaattttc tccgaaagca agtacgggct
    10021 tgccgctttt ttgaaaagtc cctattgcta aagacatttc ttccaaagca ggataattgg
    10081 tcataatcat ttggggctga agaagtattc ctttaatacg ctcatctttg gcagcaagtt
    10141 ttattttagc gcaaatttcc tgaacactgg gagaagaaac atttacaaag cgtaattctt
    10201 ccagctcgtt atagtccgga agtaaagcac ttggatttac atataaccaa gcattggagg
    10261 gaatttttgc agatttggct ccgtgagaac caaaagtgaa tataaaaccc aggataaagg
    10321 cagctaataa ggcaataaca aagattaaac agccccacag aacggtcttg tttgatttca
    10381 taatatttaa actcctttta gcgactaacg aatgccgcta ctgataatcc gcataacact
    10441 gatattataa ataactttca gagctttggc aaattggaat ttggcaacct gctttcctgc
    10501 tggagtattt atatccttca ggttaaaaaa aatcaataaa aatggcggag atgtagggat
    10561 tcgaacccta gaaacgctca cacgtttaca cgatttccaa tcgtgctcct tcagccaact
    10621 cggacacatc tccttgctta tttttcccaa ctttttttct tacctctttc tgtcaaatag
    10681 aattagtatc tgctcacctt ctcactaaat aaaaacacta tatctgctaa taaggaatat
    10741 cattttatca gtaaatgttt ccacaatgcg cagaaaaagg ttcatatatg tatattagtt
    10801 atttgtaaga tgccctaaat gtagttataa ttctattaat ttttttagaa ggtcttgagc
    10861 ttttttaatg tctattgaat tatcaggatt taagatcagc tttttaatta ccttaccatc
    10921 aaactgagtt acccctcttg aatttctacc tgaccaatga gaaaggataa aaatagaatt
    10981 acacagatct ttttgaaaca tagatataag cgttgtcttt acgctatttt ttaaatagtc
    11041 tatgcctaca aaatagaaca taaaaactgt cctttccttt gtcaggaact ccagaatttt
    11101 atcaatatta taagctttag gatttgaatc taaaaccatt atctttgttt ttacatcagt
    11161 ttcagttaga aaattagcaa attcccttga ataatcacct aaagaatgtt tattagatgt
    11221 gagggattga taacctttcc ctgaattaag agatttaata atttctttac gcaaggaatc
    11281 gtcatttccg gcaataagat actctataac agcgcttcta atattgctat tctctattaa
    11341 agaagctata agaatttcgt ttctatattg ttttacccga gcatcaagtt cttcttttag
    11401 gataatatat tcattactgt taacaaaaga ttgagctctt agaggagcag ctaaaatttg
    11461 tttaatttca ctatctttta attctaattt tttaccggaa ggatgaatat cattagtagc
    11521 ttcaaccaaa cgtgcaagat tttcttcaaa actaaattgt ttatggaagt cgaataaaac
    11581 agcgaaatta tcaggttcat ttttatagcc attaaattct ttcctaatat ctgatccatt
    11641 gaagctgcct ttaatattat tagttcgcaa cagctgagag ctatggctta tcttagataa
    11701 aaatgtagaa ttagcaagat acatataatt cttatcggga gttactacac atacgataaa
    11761 aggtcggtca tcaacttttc ttaagtttga aagagagaga attgtattgc taagattggg
    11821 gcgagcagat ttacaaaacc tgattgcaaa ttcatcattg taatacacgg acctgtcacg
    11881 aattaatttg aatcttcggg aaacaagttt agttagttta ttcttatcct taattgcgtt
    11941 ttttgattca atgaacttaa tgatgccagt caaattcgat ttcataatta tcaattcctt
    12001 tttgttttct acttgatagc ttaatttttt gctgttcggt taaactattt ctctcccaaa
    12061 ataatccttc actgtatcct tgtatagatt tatcaatatc agctaagtct aatcgtttta
    12121 aggtaagagc acaataatat tcgtcaattt caataccact gaattcgcgg tttaattttt
    12181 ttgctacaac taaggttgaa ccagagccac aaaagggatc aaacacaaaa tcaccttcat
    12241 tggaactggc aagtattagt ttcgctaata atttttccgg tttttgagta gggtgatcag
    12301 tattttcagg catagaccaa aaaggtatag tgatatctgt ccaaaagttt gagggatgag
    12361 ataatctata attgcctgta tttgtttctt tccagtcctt aggatctccg ttttcatctc
    12421 tataaggtgc aattactttt cgcataattt taaccttatc tacattgaaa acaaatttat
    12481 tggacttagt agcaaaccat atatcctcag aacagttttt ccagtttgtt tttgctcctc
    12541 tacccttatc tctttcccat gtaattctat ttcttacaat caaatgtttt cttagcactg
    12601 tctctattgc agaagaagat tgccagtcac agcatacata aaccgaaccg gttaatgata
    12661 tttttgaaac aatctgagaa aaccaagagt caatccattc tgcatactca tcaatggttt
    12721 ttttattgaa ggtaacttca ttaaactttt tatacatatt atagggggga tcaataataa
    12781 gaagatcaat tgatttttcg ggcaggaatt gaatacattc aagtgcatcc tgacagatca
    12841 ctttcccttt tatagcatct aacatacatt gcttatttaa gttgataaat cttgttttca
    12901 ttttttttag ttcctcttcc gacacagtaa gagttctatt tcttggtgct gtatgtttca
    12961 ttttatcttc ccctcctatc ttattattag acaaaggtat taaatcagaa atattttttt
    13021 ctgacttttc gtcttttgtt ggcgcaaaat ctatctcata attatgtata tttttagaga
    13081 ccattttagt atcctctctt caacgattat attggattta atttcatttc attatatttt
    13141 tctattatta ctccatcttt ctgtcaactt ctttttttca tcttcactta ataacatatt
    13201 ttcagtgttt gaaatgacgg actccggtaa agaccatagc gatgtttagt tcattgcagg
    13261 cagaaataca ttcctcatcg tttttagaac cgccaggttg gatgatagct gaaattccgt
    13321 tcttagcaag ggtatcaata gaatcccgaa aagggaaaaa gccatcggag gcacaaacag
    13381 aatccgttaa atcgtgtccg aatttttttg ctttccagag agctaattga acggcatcta
    13441 tgcgggaggt ttgtccacag ccaagaccta ataccctatc ttttttaacg atagcaatgg
    13501 cattggactt taggacggaa acagctttcc aggcataaat aagggcttcc aactcttccg
    13561 gagaaggttg tttgttagta actattttcc agttctctgt tgattcgttt acgaggtccc
    13621 agtcctgagc taaatagccc caagtaagtg tcttaatttc tatagggtta agcggttttt
    13681 ccagcagaga aaattcgtag tgaattaggc ggcggttttt cttttttttc aggaattcta
    13741 aaacaccggg ttcaaaagca ggagcaataa ttatttcagt gaagatatta ttaatcaggg
    13801 tagccgtttc caaatccagg ggacggttca caattacaat tcccccaaaa ggtgcctccg
    13861 tatctgtttc ataagctttt ctatatgcct ctgccaatgt ttcatcactg ccaataccac
    13921 aaggattgca gtgtttaata ataatggcag ttggtttacc aaaaagacga atagctctga
    13981 aggcagaatc aatatccatt atgttattga aagagagttc cttaccgtga ataagttccc
    14041 aaccggaggt tacctttgca tagaaaccac cattttgatg agggttttcg ccatagcgca
    14101 attttctctt taaagaaata gcaaactcca gttgagcagg tagttcgtta gcaatagatt
    14161 cctctattcc ttgagaggca aaataagcgg caatttcagc atcgtaatcc cgggttttgg
    14221 caaatgcctt ttgtgccaga taggaactaa atttttccgg taaaatacta ttctgcttta
    14281 aaagctccaa cgcaggagca tagtccacag gatcaaccaa aacggtaacg cttttatagt
    14341 tttttgctcc tgcacggatt aaagcgggac ctccaatatc tatattttct ataatttcct
    14401 gttcggtggc attttctttc cagcaaacag aggcaaaagg atacaaattt accgctatta
    14461 catctatgtg ctcaattttt agttcagaga gtgttttggc gtgagcagga ttatttctat
    14521 ctgccaaaat tccggcataa ataacgggat ggagtgtttt aactctgcct tccagaattt
    14581 ccggaaaacc ggttaaatcc gaaaccagca ctactttctg gcagaattgt tttaagtaat
    14641 cggctgtgtg cgaagtggaa agaatattgt agcccatttt ttccagttcc accgctaaat
    14701 attcaattcc ggttttatcg gaaacactaa ttagggcata tttattcatt tatcatcttc
    14761 ctcgttcagg gtatctgtca atttcttcag ttcttcttta ctcaaaattc cttccaggcg
    14821 ttgattgagt ttttgattag taaaaagttc cattgttatg cgcacagcat tatgaggttc
    14881 tttggttaaa aagccaccgt tttttttgtt ttccatttca cttactaaaa ccattcctcc
    14941 caaaataata gcccaattta tataaatcca cagcaggaaa atcggcagaa cagccatcac
    15001 tccgtaaagg cgctcataat tagttaagtt atatatataa aaatcaaatg cggacttacc
    15061 tataacccag ataaccgtag tccaaaaagc acctctaaac aaagaggcac ggttaattct
    15121 taccgaaggc aataacatat aaagaaacat tatagccaaa aattgcaaaa taaagggaac
    15181 tattaccata atccattgta aaacaggtga ctttaacaaa cgggagataa ttggaataga
    15241 tgaactggaa aatagcaaca cgataatcag caatccgaaa acaatcgtgc cgaaaaattt
    15301 cactaattga gtaatagtat cctgagagga cttcagctgc atactcagga tacggtcaaa
    15361 agtccctctg ataactgaaa acaaggaata ggaactaaca atcaggataa caaaattgaa
    15421 aatgttgaag ccaactttgg gcataatcag cttgtccaaa aagttgataa tagcatttgc
    15481 tgaactcgga atgaagtttt gaaccactaa ttctctcagt ttatcaccca aattcagaaa
    15541 tggtaaatcc ggggcaatta aaaccagaaa attgataaaa gggacaaaac cgagaagagt
    15601 tatgtaagtt aaactggctg attccttcaa aataccttcg cccataaaac gctggtaaaa
    15661 tgttttgata aaagtccata tattgccaaa gacctttttc cgttgttgag ggacagttat
    15721 ggactggtaa attttcttga ggtccgaaat aacaattttg atgtaagaat atagccaaag
    15781 aaggagcttc tgaaaaacac ctttattttg tttcttttta ctcattattc ctcccttaaa
    15841 gcaattacgg gatcaagacg ggcagctctg acagcaggac caaccccaaa aataagtccc
    15901 actccaatag aaactaaaag agcaacccaa atcatttgca ccgaggcaag cacttttatt
    15961 tgtaaataag aacccacttt atccaaaata gcaaatccta aaagaattcc caaaattcca
    16021 cctaaagcgg taataagcac cgtttgcacc aaaaactgaa taaaaatatc tcttcttctg
    16081 gctccaaccg caatgcgaac tcctatttcg cgagtgcgtt cccggatgga agcaagcata
    16141 atgttcataa ttacaattcc cccaaccagc agagaaatta cggctattaa aacaaatatg
    16201 gcttcaaata ttttagcatt tgcctgcatt gtatccagtt gttctttagc ggaagtaacc
    16261 tgaaacaaat gttttccgtt tttcaggttc agcactatgt tttccacttt acggcgcagt
    16321 tcttttgcct cttcaggact ttttgctcta atatctatgg agcttatttt actgccgggg
    16381 ttaactttac tgatcattgt ggaaatagga ataaaacaac ggcggtttaa atattccaaa
    16441 gcattttcac ccattcctaa ttgtgcaccc tgtgcttgca taaatctttc agccattaca
    16501 ccgacaacga taaattgttg attgcttacg gaaataattt gtccgagagg atcgttgttg
    16561 ccaaaaaact cttttgccac ggttgagccg ataactatta cattgctatg ttggtctata
    16621 tccaggtctt taataaatct gcctttagct atgtcccatt gttccacttt aacaaaatcg
    16681 ggcattacac ctcttaaaga tgtgctgtaa cgaatatcac ccttatttat taccgcttcc
    16741 cattcctgaa ctgtaggatt aaatgcttcc gcttccggaa taagggattg caaataacga
    16801 atttccttta aagaaaaact gggatcgcca cctttggaaa aatcgtaaaa ccagttttgt
    16861 tcaacttcta ttttgttcaa accaccgcgt tcttccatcc aactcattgt gctttcgttc
    16921 attccattta caattgccag aacaaccatt atgcacatca cacctaaaat tatgccgatt
    16981 acggttacta tgctgcgaac tttacgcatc ataatatcag cgaaaccaag ttttagggat
    17041 tcagctattg gaattgccat ctactatttt cccgtctttt attttgatta ttctttctgc
    17101 tcgggaagca attgcctggt cgtgagttac aataataacg gtattgcctt gactgtgtaa
    17161 ctccgtaaaa atttccaaaa tatcgcctcc ggattgagaa tccagatttc ccgttggttc
    17221 atctgccagt agaatagaag gttcatttac aattgcccgg gctatggcaa cgcgttgtct
    17281 ttgacctccg gaaagttcac cgggcttatg ttttaaacga tctcccagcc ccacattttg
    17341 taaaacctct tttgctttgg cagtgcgttt tcttttactc atacctccat acattaaagg
    17401 gagttctaca tttttcaaga tgttcaaatg agggagcaga ttaaaggact gaaatacaaa
    17461 gccgattttc cgatttctga ctgcagctaa agatgctttg ggcattttgc taaccaaaac
    17521 atcatccaga taataagttc catctgttgg actatccaaa catccaagaa tatgcattaa
    17581 agtgcttttc cccgagccgg agggacccat tatggcaaca aattcacctt tctgaatttg
    17641 caggtcaatt ccatttaagg cacggacttt tatcttgcct aaaatgtagt cctttactat
    17701 gttttcggtt ttaataatat ccatatttac ctatttttcc tttataatcc tttcacttat
    17761 tatctgctat tgtgtcaatg aaaaagtgaa tctctcacag ataacacgga tttcacagat
    17821 ttctatgcaa gttaggagtt gtaattgctt tcaaaagtta taaaccttat acccctttat
    17881 ttaacagtaa ggactcataa cgagttagca cgcagatttt gcggatttta ttataataat
    17941 tttgcctttt tttgtctatc gtgggtttct tctttttatt ttttcacctt ttcacctgtt
    18001 cacccattaa cctgttcacc ttttttaaga tgaattattt ttcttaaggg aaatactgaa
    18061 gcattgctta cccattacgg atggcattag caatgggtaa gcaatgagta agcaatgcaa
    18121 ttagggaaga atgtatattt atttctattt atatgaagtt agagaaattt agggatgaaa
    18181 ctggtgttgc acttaaaaat tccattgaca tcagctttcc aatttttcat tttatccctc
    18241 caattgttta aacccttcaa aacacttgac actattcctg cttccaaaat tgtgactttc
    18301 tgaataaaaa aatatgtaaa ggagaagcga tgaagaccct aacccctagt ccttccgata
    18361 ttcaccagca atggtatatc gtagatgcag agggaaaacc tttgggacgc ttatccacaa
    18421 aagttgccac aattttatgt ggcaaacata aaccgtatta tgtgcccaat attgataccg
    18481 gtgattatgt gattgtgatc aatgcagcaa aagttcgcgt aacgggtatg aaggcattac
    18541 agaaggttta taaaagcttt agtggttatc ctgacggttt gaaggaaatt ccttatgcca
    18601 aagttttgga agagcatccc gaacgcatta ttgaacatgc cgtaaaaggt atgatgccca
    18661 aaaataagtt aggacgcgca atgttcaaaa aattgaaagt ttatcctgga gctgaacatc
    18721 cgcattctgc tcaaaaaccc attgaactaa atatttagga gtaaaaaagt atgcagacct
    18781 ttgatgcagt tggcagaaga aaaaatgccg ttgccagagt tcgtcttatg cctggaactg
    18841 gaaaacgcat tattaataat atccagatga agaaatatct ccagcgtgag accctggaaa
    18901 tgattgtaga acagccctta cagaccgttg gcttatccga taactttgat gtttatgtaa
    18961 atgtttacgg aggtggttta agcggtcagg caggagctat ccgtcatggt atttcccgtg
    19021 ccttagtgga atatgatgaa aatctgcgtc ccgtgctgaa gacccgggga tttttaacgc
    19081 gtgatcccag aatggtggaa cgcaaaaaat ccggtcgtcc caaagccaga aagagattcc
    19141 agttctctaa gcgttaaaag aaacagtttt tctgtaatga aaaacaacaa gtgttataag
    19201 ttccctattg atagttcagt taaagagatg aaaaattatc tgcttcctgc tttcacagga
    19261 atgacaaaag gattatcaaa atggaaaaaa cattaaataa acttccggaa cgcttgcaag
    19321 gcggtgaggt ttttgttcct ttgaattgct tacaccgtcc ggaagagacc taaaaaccgt
    19381 atttcaggag aaaagaatgt ccgtagtaac tatgaaacaa ttacttgaag cgggtgtcca
    19441 tttcgggcat caaaccttca aatggaatcc taaaatgaag aaatacatct tcattaaacg
    19501 caacgggatt catattattg atttgaaaca gacagtagat gctatcaatg aagcatatca
    19561 gtttatgaaa gaagttgcct ccaaaggtga atatatcctt tttgtaggaa ccaaaaaaca
    19621 ggcacaggca gctattaaag aag
//
LOCUS       DAOPFL010000026        19337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_3075_length_19337_cov_84.244010, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000026 DAOPFL010000000
VERSION     DAOPFL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 19337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19337
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3075_length_19337_cov_84.244010"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5141
                     /locus_tag="PLG69_03310"
     CDS             <1..5141
                     /locus_tag="PLG69_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42568.1"
                     /translation="NTASAVGDTIYYSNNMRPPYEPWYDELIEGTDFVTDYDSGIITI
                     LKTIDRTATLGVQYTRRDGVQVPPPNSDTSRLHVKVLRKKYQEYIPYDPNDELNTWHL
                     QMRNIYDMGRTNIKNDGFELQVYTVNVDLTRNYNLPDNLVFGDISTYNDYLRLNANGS
                     PDGLINGDDATVNLTNGWIIMPFIEPMRGLGDGIIYRKENEDEYSYQDSTSIFINIKG
                     KIGREAIELSSMGVLKGSVKVKVNGNEQRENIDYIVDYDFGRITFLTAAGKDPDAKIE
                     IEFESRTLFSVAQKNLAGIRANWKLSDYAKLGGTLIYRSEDVSDRRPRIGNENIEMLM
                     GDVDGELTFKPAFITKWLDALPLINTTAPSQLSLSGEVAFTMPNIYGDPKSKKKEAYL
                     DDMESIMDSYPLGVTYNTWVLGSKPFSTSYAKGRINWFNPKDVKRKDIEAEATLTEQE
                     KDEYVTVLTMIVKPNPVQIPGSTTHSWAGIMKYLGNQLDFSQKKYLEVYTKLEPLYPN
                     TNIYPNVTMHIDLGDINEDFYTEYGGYGVLNSEDVNRDGVLTISEDIGLDGIPKNQPG
                     HDPNDIAYPASGNDYSGVNGTEENRILDTEDLDGNGVLNTLDRYLSYSFSLTSPDSSL
                     VVDSYNQWRMFRIPLNDEQYYQMVNSSGTNIQPSLQKISYARIWLDTDAQMDVKVRIA
                     DISVVGNKWQDNFIRQFKYGNAPPPNSYVQYYNPIIEPSVLIAKNTSYISGIVTNQKN
                     FTHYTSPAGTWYTEEKKESAESALTLEVNHLQRDQMCLLRQRMLDTQNLLSYDKIRFW
                     VYPEAAQGSYSNLDSLYIIFRIGADSLNYYQVRQRVPVRPYNAKMNANDWIQLEYNLQ
                     DLISLKEINPNAEADSLVNIIPGDEGQLGKYYRGKPSLINIRDIYLGVYVPGVHFYAN
                     QMDIKEFSGIIYFNDIRVADPYEDLGIAKRLSVSGTFADVATINVDFEDKSENFNTTI
                     QRGRSNIFTSTQSLNITNKIFLNKLFPNSWNLDMPLSLTRNYSLGTPRFRANSDLLRA
                     NIADPELKEIEKTERLAYSADFGFSQKQAPKNKILQYTVYRTSLSGRIESSYNNTSTA
                     VDTILAYRGTLNYNLNVPAEKTSFKLFKNYRLSYFPNTFSNSITFSSNEPKSWDRLTT
                     VDSLVWNKRSQTTDTRTITTDNNLSWSLLSDLTATARVNTKRDLLQKDYLYDINIGKQ
                     TEYVQDLGLNYSPNYLPQVCNFTSSVSARYTDTQRKYTQYVESQAVDTYQRDGNTNRS
                     IRMNLTLMNSSLLSNWAIKMKSKQPPESVSPKGKEDKGSAKTEMTEEDKKKQEEQKKQ
                     EEMKKEDEQKKQEEIKKEEEQKLSEEEIQKRKDELARLEAEGKLADLSEEELNKLMED
                     IFGKGEKEEKTEEEEKETETTKPSETKEPGFNPVITLVNALAMLKNITASYQNTYMMN
                     YARKTNPFPFSFQIGLPHTVPYDSLEAISNDNTLTLGSGITFSRRVDSIINCSLMSNR
                     RYASASNQTIGYTFPDITLSVMDIETLLGLGKYISGSRLNTGFQYTVRQNGNLDWVKP
                     KQESYTYALNPLLGFTGNLFKVVSTNLSFSLSQTKNITDMDTYEILKTSNTQSLNGNI
                     SYSFRAAKGFSVPFTKKKIHIKNELTSSLGITYENNFDKTKGSTTSQVDRNTSRFAIS
                     PQATYQFDVNIRGGLTGSYEVNSDKKTEDGTSIFSLGVWVEINL"
     gene            complement(5169..6053)
                     /gene="era"
                     /locus_tag="PLG69_03315"
     CDS             complement(5169..6053)
                     /gene="era"
                     /locus_tag="PLG69_03315"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPI42569.1"
                     /translation="MNNSANFKSGFVTIIGKPNTGKSTLMNLILGEKISITSPKPQTT
                     RYAIKGIWNTSEHQIIFVDTPGYLKPRYELQEKMLKIWHNALKDVDLIILLTQIDGFP
                     TEYDKEVLNQLKTLKNPQLAVFNKLDLNPEVDRNELVKYLPESINEVFFVSAKTGENI
                     PELMEAIKYYIPFHEPYYDNDMLSDLPMRFFAQEIIREAIFHFFEEEIPYSSAVLIER
                     FKEFTDKVIIDAVIWLERESQKPILIGKNGSTIKKVREYAERELTRFMQMPVQVHLFV
                     KIKPKWRKKQSALRELGF"
     gene            complement(6099..7433)
                     /gene="rocD"
                     /locus_tag="PLG69_03320"
     CDS             complement(6099..7433)
                     /gene="rocD"
                     /locus_tag="PLG69_03320"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPI42570.1"
                     /translation="MSTNLKCDCSTDNEIVEVEKNYGGKMSQNLKYGNISSSEAMELE
                     EKYGAHNYHPLPVVLAKGEGVYVWDPEGNRYYDFLSAYSAVNQGHCHPKIIQALIDQA
                     KELTLTSRAFYNNKLGEYEKYITEYFGYDMVLPMNSGAEAVETAMKLARKWAYNVKGV
                     ASDSAILIFADNNFHGRTISIISASSDPDCFGGFGPFTPGIVRIPYDDANALKEYLEK
                     NGKNVAAFIVEPIQGEAGVVVPKDGYLKDCYDLCHQYNVLFIADEIQSGLGRTGKLLA
                     CDYENVRPDVLILGKALSGGVLPVSAVLADKDIMLQIKPGEHGSTYGGFPLACVVAIA
                     ALEVIKEEKLTERAFELGEYFRNELRKIEHPMLKLVRGKGLLNAIVIEPMNGFEAWDV
                     CLKLKDKGLLCKPTHRHIIRLAPPLIITKEQLDECIQIIKDVFNDLSANLGQ"
     gene            complement(7498..8841)
                     /locus_tag="PLG69_03325"
     CDS             complement(7498..8841)
                     /locus_tag="PLG69_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPI42571.1"
                     /translation="MKHSKIEILEKTPVAYSILYLALPSMLSMLVNILYNLTDTFFIG
                     KLNDPFKVAGVSIALPYFNMLMAIAGIFANGGASYLSRLLGKKDLKTARETTTTAIFT
                     VAIVSIFAATLGVLFIPTYLNLSGASALTALSARKYLTAIFIGSPIIMIKFTLIQLLR
                     AEGSAKEAMLGLFIGTGANIILDPLLIFTFKMDVTGAAIATVIGQGLAMLYYISYYLK
                     KKSLASPGRKYLHPRWSCYKEIFAIGIPSSLSQIMMSIGNAISFKLASAYTDHTIAAL
                     GVVSRVFSIVIFAFIGLSIGIQPLIGFNYGAQNYTRMKKVIKMSSIICLGMSAVFTLF
                     FALFPEELIAVFIKDKAIMKIGTQILNAYVIAIPFAGLGMILMAALQAMGKAIPAFIV
                     SLSRQGIVYIPMLYLLNYLFQFSGLIYAIPIADVITTILSFTFVYHITKRLKSSP"
     gene            9205..10074
                     /gene="htpX"
                     /locus_tag="PLG69_03330"
     CDS             9205..10074
                     /gene="htpX"
                     /locus_tag="PLG69_03330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HPI42572.1"
                     /translation="MNFLKRIGIFLLTNILVMLVLGIVVGLLGLKMEDLWGLLIICAI
                     FGMGGSLISLFLSKPMAKASYKIQIVKPGIAHPKIAYLLSAIESMAEERNIKMPEVGI
                     YNSRDANAFATGASQNNALIAFSSALIDRLSEEELAAVAGHEMTHITNGDMVTMTLLM
                     GVVNTFVMFFARILTVVIDNALRDDRGGGLGYFGYIMVIMVLQNILMLLANIPIAAYS
                     RWREFKADAGSANLVGAGPMIEALKKIANAYVPEKRSDSYALAKINNQKRISLFATHP
                     PIEVRIQHLQEML"
     gene            10071..11441
                     /gene="rlmD"
                     /locus_tag="PLG69_03335"
     CDS             10071..11441
                     /gene="rlmD"
                     /locus_tag="PLG69_03335"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPI42573.1"
                     /translation="MKHILNLEIEKMAMGGFGIAYAEGKAIFVPYTAVGDVVDIDITI
                     EKKDYAYGKVTKYISYSDSRVEVQCSSFAAEIPCGGCDWLMLKYSEQLKQKNQLLLEL
                     FSPFIDKDVIYFPAPSTQIKNYRNKVFMPVGKNYQTGKITYGMFARFSHQIVEHDTCE
                     NHPPIFDQLANSLMEFCLKAKVEPFNEIEHKGQLRHIGFRCNSDSRQILVILVTLSGK
                     LPFTNLLVKKLTTEFPQIKGIIQNINRQKGNVILGKETKLLYGSENLYDNLCGLKFRI
                     NYRSFWQINTGTMEMIISFLQDFVNSNDVILDAYCGIGAIGLSLADKVKRVILLEEFP
                     QAIEDAKENAVLNGLNNVSFVVGKVEDNLSAVLIKEKPDTIIMDPPRSGVQQNALEAI
                     IQAEVKQIIYLSCAPMTLARDLNTLIGGEKYKVVFLQPFDMFPNTWHIECLAVLKYND
                     YNINMR"
     gene            11465..11896
                     /locus_tag="PLG69_03340"
     CDS             11465..11896
                     /locus_tag="PLG69_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42574.1"
                     /translation="MKRYLYLIFGILLMLALGSCESNDSDTIAQREIRDILYDISMEF
                     NLKNISGIMEHLHIKYLHNGMTSYNFNDLWLDRMAQYSLLEIEVLYIDLDDDYAVVHS
                     KNKFTSHAETVTLNEPEDSGDISYFKRENGVWYIYGNQRLY"
     gene            complement(12251..13021)
                     /locus_tag="PLG69_03345"
     CDS             complement(12251..13021)
                     /locus_tag="PLG69_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPI42575.1"
                     /translation="MPDILFRLVYKKGKQNCGLNMDYIQHYHNDALEFDYWGKDLITP
                     AEIRRNQFIQHLCPIKKGQKVLDIGSGRGWFSLHCANMEAEVTAIDLSEENLNRIKNI
                     DPRIKTIYGDACSINLPGKSFDLIVALEVLEHLVDPALAIDNWKKMLKTGGILFVTVP
                     YKEVIRYSLCIHCNRKTPMNAHLHSWDAPKLASILENNGFYILRATLFAHKLLSALGL
                     DKKLQKVPFRVWNFLDKLCGIGNDKYSYLAVKAIRQNR"
     assembly_gap    13387..13486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13821..14147
                     /gene="rpsJ"
                     /locus_tag="PLG69_03350"
     CDS             13821..14147
                     /gene="rpsJ"
                     /locus_tag="PLG69_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPI42576.1"
                     /translation="MSKHDNRIRIKLKAYDHRLLDKSVAEIVKSTRNTGAKVVGPIPL
                     PTDRTIYTILRSPHADKKSQDQFQMLVHKRLIDIVNPTQQTTNALKKLSLPAGVHVEI
                     KANTRS"
     gene            14150..14782
                     /gene="rplC"
                     /locus_tag="PLG69_03355"
     CDS             14150..14782
                     /gene="rplC"
                     /locus_tag="PLG69_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPI42577.1"
                     /translation="MIGLIGKKIGMTQVFDSDGKVIPVTVIKAGPCKVICKRTEAENG
                     YEAIQVGYEEIPARKVSKPLQGHFKKYNSPFYRYVREFRPSYGQHIESVQIGDELKAD
                     IFGENETVSVSANSKGRGFTGVMKRHNFNGFISSHGSHESFRGPGSIGQCAQPSRVFK
                     GMKMAGQYGNTKVTVHHLKVIKVDPENNIIMVKGAVPGHRNSLVTIHKEQ"
     gene            14793..15428
                     /gene="rplD"
                     /locus_tag="PLG69_03360"
     CDS             14793..15428
                     /gene="rplD"
                     /locus_tag="PLG69_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPI42578.1"
                     /translation="MLTAKRYNMQGELLGEVDLPEGIFEVDVNAPKILLNEVVTMFLA
                     NQRQGTVQKKNRSMTAGSTRKLFRQKGTGNARVGTRRSPIRVHGGKAFAIYPKDWYRK
                     IPRTKKRLALKVALTDRAREGRIMIVDNLSFEKPNTKLAVELLNKIIPEKGYKLIVTD
                     GHHIPTVKSFANLPEVMTDRADSLNAYEVLKSSYIIMTDEALKKVEEVFNK"
     gene            15425..15712
                     /gene="rplW"
                     /locus_tag="PLG69_03365"
     CDS             15425..15712
                     /gene="rplW"
                     /locus_tag="PLG69_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPI42579.1"
                     /translation="MIHPRNIIISPIITEKSSNQVQNENTYSFKVSSNANKIEIKKAI
                     ERIFAVKVLAVNTIRYKGKPKSLGKYNGKRPDWKKAIVTLREGDKIADFEV"
     gene            15724..16551
                     /gene="rplB"
                     /locus_tag="PLG69_03370"
     CDS             15724..16551
                     /gene="rplB"
                     /locus_tag="PLG69_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPI42580.1"
                     /translation="MGIKKYRPTTSSLRFRTGHTFEEITKKTPEKSLLKPKHRTGGRN
                     NSGRITCRHRGGGHRRHYRIIDFKRDKIGIPAKVAAIEYDPNRTARIALLHYVDGEKR
                     YIVAPDGLEVGAKVMSGPDADIAVGNAIPLEKIPLGSVVHNVELKRGKGGQIARSAGT
                     FAQVVAKDGDYVHIKMPSNDVHLIRKECLATIGSVSNPDHSLIQIGKAGRKRWMGIRP
                     SVRGVAMNPVDHPMGGGEGKTSGGGHPVSPWGKPAKGGKTRKTHKYSDKYIVKAVKK"
     gene            16568..16846
                     /gene="rpsS"
                     /locus_tag="PLG69_03375"
     CDS             16568..16846
                     /gene="rpsS"
                     /locus_tag="PLG69_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPI42581.1"
                     /translation="MARSIKKGPFVDAHLLKKVEVLNAEGKKSVIKTWSRRSTITPQF
                     IGHTFSIHNGHKFVPVYITENMVGHKLGEFSPTRTYRGHKEKKKKVTR"
     gene            16857..17201
                     /gene="rplV"
                     /locus_tag="PLG69_03380"
     CDS             16857..17201
                     /gene="rplV"
                     /locus_tag="PLG69_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPI42582.1"
                     /translation="MEAKAKLCYARGSARKARLVLDTIRYKPVTEAQNILRFSRRRVA
                     GTINKLLASAIANAQHQDPKVNLDKMYVITATADEGPQMKRYMPRAMGRAYMIRKPTC
                     HISLEIQTIEEE"
     gene            17212..17859
                     /gene="rpsC"
                     /locus_tag="PLG69_03385"
     CDS             17212..17859
                     /gene="rpsC"
                     /locus_tag="PLG69_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPI42583.1"
                     /translation="MGQKIHPVLYRLGVNKETESIWFAQGTSYVDFLQEDIKIRNYIR
                     KRLSDKMVSRVQISRKTSSIIIDIYTARPGLVIGKKGEDIDKLRNELNVFINKDRKNP
                     ISVSINIEPIDKMWLDARLVAQEIARQLEERISFRRAMKMAIRYVMKENAQGVKVQVS
                     GRLGGAEIARSERYKQGRTPLHTLRADIDYANVEANTTYGVIGVKVWIYKGDILD"
     gene            17872..18291
                     /gene="rplP"
                     /locus_tag="PLG69_03390"
     CDS             17872..18291
                     /gene="rplP"
                     /locus_tag="PLG69_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPI42584.1"
                     /translation="MLAPKKVKYRKMMKGRRKGLSWTGCNISFGDYGLIALEDSFITS
                     RQIEACRIAITRHMKREGKVWIRIFPDKPITKKPAETRMGKGKGAPEYWVAVVRPGRV
                     LFEIEGVDVNVAKEALRLAAHKLPIKTRFIAREGVEL"
     gene            18288..18488
                     /gene="rpmC"
                     /locus_tag="PLG69_03395"
     CDS             18288..18488
                     /gene="rpmC"
                     /locus_tag="PLG69_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPI42585.1"
                     /translation="MKIEEIRDLSIEELQAKLEELRIELFNLRFQKTKNLLDRPDRIR
                     NIRHDIARINTILTEREKEKKI"
     gene            18515..18751
                     /gene="rpsQ"
                     /locus_tag="PLG69_03400"
     CDS             18515..18751
                     /gene="rpsQ"
                     /locus_tag="PLG69_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPI42586.1"
                     /translation="MIKQGIVVSDKNDKTIVVRVERQFIHPLYKKTVRRHKKFMAHDE
                     NNSAHEGDIVQIMESRPLSARKRWTLHKIVERSK"
     gene            18767..19135
                     /gene="rplN"
                     /locus_tag="PLG69_03405"
     CDS             18767..19135
                     /gene="rplN"
                     /locus_tag="PLG69_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPI42587.1"
                     /translation="MIQVQTMLNIADNSGAKKAMCIKVLGGTQRKYASIGDVIVVAIK
                     SATPGGKVKKGSVERAVIVRTRKELRRPDGSYIRFADNAAVIIDEKHEPKGTRIFGPV
                     ARELREAHYMKIVSLAPEVL"
     gene            19159..>19337
                     /gene="rplX"
                     /locus_tag="PLG69_03410"
     CDS             19159..>19337
                     /gene="rplX"
                     /locus_tag="PLG69_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPI42588.1"
                     /translation="MRFKKGDLVKVIAGDEKGRKGHILKVFPKTGKVIVEKVHMIKKH
                     AKPTQQNPQGGIITM"
BASE COUNT         6341 a         3409 c         3762 g         5725 t
ORIGIN      
        1 ataataccgc ttccgcagta ggagatacta tctactattc caataatatg agacctcctt
       61 atgaaccgtg gtatgatgaa ttaattgaag gaacggattt tgtaaccgat tatgacagcg
      121 gaattattac tatcttgaaa actatagatc ggactgccac tttgggggtt caatatactc
      181 gcagggatgg agttcaagtt cctcctccca attctgatac cagtcgctta catgtgaaag
      241 ttctaagaaa gaaatatcag gaatatattc cttacgatcc taatgatgaa ctcaatacct
      301 ggcatttaca aatgcgcaat atatacgata tgggtagaac caacattaaa aatgatggtt
      361 ttgaattgca ggtttatact gttaatgtgg acttaaccag aaattataat ttacccgata
      421 atctggtctt tggtgatatt tctacctata atgactactt aagattaaat gctaatggca
      481 gtcccgatgg acttatcaat ggagatgatg ctaccgttaa tcttaccaat ggctggatta
      541 ttatgccttt tattgagcca atgaggggct tgggagatgg cataatttac cgcaaagaaa
      601 atgaagatga atattcctat caggactcca caagcatttt tattaatatt aaaggtaaaa
      661 ttggtcggga agcaattgag ctttcttcaa tgggtgtgct taaaggaagt gtaaaagtta
      721 aagtaaacgg caatgaacaa agagaaaata ttgattatat agtggattac gattttggca
      781 ggataacatt tttaactgct gccggaaaag atcccgatgc taaaattgaa attgaatttg
      841 aatcccgcac cctctttagt gtggcacaga aaaatcttgc cgggataagg gcaaactgga
      901 aattgagtga ttatgcaaaa ctgggaggaa ctttaattta tcgcagtgaa gatgtttccg
      961 atagacgacc cagaataggc aatgaaaata tagaaatgtt gatgggagat gttgatggtg
     1021 aattaacttt caaaccggct tttattacca aatggttgga tgccctgcct ttaattaata
     1081 ctacggctcc ttcccagctt tctttgtccg gtgaagttgc ttttacaatg cccaatattt
     1141 atggtgatcc caaaagcaag aaaaaagaag catatcttga tgatatggaa tcaattatgg
     1201 attcttaccc tttaggggtt acttataata cctgggtgtt gggaagtaaa cccttttcaa
     1261 cctcttatgc caaaggaaga ataaactggt tcaatcccaa agatgtgaaa agaaaagata
     1321 ttgaagcaga agcaacttta acggaacagg aaaaggatga atatgtaact gtgcttacta
     1381 tgatcgttaa accgaatcct gttcaaattc caggttccac aacacatagt tgggctggaa
     1441 ttatgaaata tctgggcaac caacttgatt tttcgcaaaa gaaatatttg gaggtttata
     1501 ccaagctgga acctctttat cctaatacta atatttaccc taatgtgacg atgcatatag
     1561 accttggcga tattaacgaa gatttttata ctgaatatgg tggttatggt gttttaaaca
     1621 gtgaagatgt aaatcgcgat ggagttctta caatcagtga agatatcggt ttggatggaa
     1681 ttcctaaaaa tcagccaggt cacgatccta atgacattgc ctatcccgca agtggcaatg
     1741 attattcagg tgtgaatgga actgaagaaa acagaatttt ggatactgaa gaccttgatg
     1801 gtaatggagt tttaaacact ttagaccgct atctcagtta ttctttttcg cttacgagtc
     1861 cggattcatc tctggtagtg gattcttaca atcagtggcg gatgtttcgg attcctctta
     1921 atgatgaaca atactatcaa atggtaaaca gttccggaac taatattcag ccctcattac
     1981 aaaaaatatc ttatgccaga atttggttgg ataccgatgc ccagatggat gtaaaagtcc
     2041 gcatcgcaga tatatccgtt gttggtaata aatggcagga taattttatc cgccagttca
     2101 aatatggtaa tgcgccaccc cccaatagct atgttcaata ttataatcct ataattgaac
     2161 cttctgtttt gatagcaaaa aatacaagct atatttccgg aattgtaact aatcagaaga
     2221 actttactca ctatacttca cctgctggaa cctggtatac tgaagaaaaa aaagaatcgg
     2281 cagaatcggc tttaactttg gaagtgaacc atttgcaacg ggatcaaatg tgtttactca
     2341 gacagcgtat gctggatact caaaatctgc tttcctacga taaaatccgt ttttgggttt
     2401 atccggaagc ggctcagggt tcttattcca atttggattc gctctatatt atttttcgga
     2461 ttggagctga ttccttgaac tattatcaag ttaggcaaag ggttccggtt cgtccttata
     2521 atgctaaaat gaatgctaac gactggattc agctggaata taatctacaa gaccttattt
     2581 ccctgaaaga aattaatcct aatgcggaag cggattcttt agttaatata atacctggcg
     2641 atgaaggaca actgggaaaa tattatcgtg gcaaaccaag cttgattaat atccgcgata
     2701 tctatttagg agtttatgtt cccggagttc atttctatgc aaatcaaatg gatataaaag
     2761 aattcagcgg aattatatat tttaatgata tccgagttgc cgatccttat gaagatttag
     2821 gaattgccaa acgccttagt gtaagtggca cttttgctga tgttgccact ataaatgttg
     2881 attttgagga taaaagcgag aattttaata ctacaattca acgcggacgc agtaatatct
     2941 ttaccagcac tcaatccctc aatattacga ataaaatatt cctgaataag ctgtttccca
     3001 attcctggaa cttggatatg cctttgtctc ttacccgaaa ttattcttta ggaactccca
     3061 ggtttagagc aaattccgac cttttaagag ctaatatagc agaccccgaa ctgaaagaaa
     3121 ttgaaaagac agaacgtttg gcttacagcg ctgattttgg tttcagccaa aaacaggcac
     3181 ctaaaaacaa aattttgcaa tatactgtgt atagaacttc ccttagcggg agaattgaaa
     3241 gctcttataa taatacttct accgcagtgg ataccatttt ggcatataga ggaactttga
     3301 attacaacct gaatgttcct gctgaaaaaa caagcttcaa attatttaag aactaccgtt
     3361 tgagctattt ccccaatact ttcagtaata gtataacctt tagcagtaac gaacctaaaa
     3421 gctgggaccg actaactacc gttgacagtt tggtttggaa taaacgttct cagacaacgg
     3481 atacaagaac tattactacc gataataatc tctcctggag tttgttaagt gatttaaccg
     3541 ctacggcacg ggttaataca aaacgcgatt tactgcaaaa ggactatctt tatgatatca
     3601 acattggtaa acaaacggaa tatgttcagg atttaggttt gaattatagt cctaattacc
     3661 tgccgcaagt gtgtaatttt acttcctccg tctctgcaag atataccgat actcaaagaa
     3721 aatacactca atatgttgaa agtcaagcag ttgatactta tcagagagat ggcaatacaa
     3781 accgttctat acgtatgaat ttaaccttga tgaattcgtc tctcttatcc aattgggcaa
     3841 taaagatgaa aagtaaacag ccacctgaaa gtgtttctcc taaaggtaaa gaagataaag
     3901 gttcagctaa aacagaaatg actgaagaag ataagaaaaa acaggaagaa caaaaaaaac
     3961 aagaggaaat gaaaaaagaa gatgaacaaa agaaacagga agagatcaaa aaagaagaag
     4021 aacagaaatt aagcgaagaa gaaatccaaa aacgcaaaga tgaacttgct cgtttagaag
     4081 cggaaggtaa actggcagac ctttctgaag aagaattgaa taaacttatg gaagacatat
     4141 tcggaaaagg tgaaaaagaa gaaaagacgg aagaagaaga gaaggaaact gaaaccacta
     4201 aaccctcaga aaccaaagaa ccaggtttta atcctgtaat tactcttgtg aatgctcttg
     4261 ctatgcttaa aaatataact gcttcttatc agaatactta tatgatgaat tatgcccgta
     4321 aaaccaatcc ttttcctttt tcttttcaaa ttggcttacc acatacagtt ccttacgatt
     4381 ctctggaagc tatttccaat gataatactc tcactttggg tagcggaata acattttctc
     4441 gtagggtgga tagcattata aactgttctc tgatgtctaa cagaagatat gccagtgcca
     4501 gtaatcaaac tattggctac acttttcccg atattacttt atctgttatg gatattgaga
     4561 ccctgcttgg tttaggaaaa tacatttccg gttcacgatt aaataccggt tttcaatata
     4621 ctgtccgcca aaatggaaat ctggattggg ttaagcccaa acaggagtcc tatacttatg
     4681 ccttaaatcc cttgctgggc tttacgggta atctttttaa ggtcgtttct accaatctca
     4741 gtttctcttt aagccaaacc aaaaacatta ccgatatgga tacttacgaa attctgaaaa
     4801 ccagcaatac acaatcactg aacggaaata tttcctacag tttcagagca gcaaaaggtt
     4861 tttctgttcc cttcactaaa aagaaaatac atattaaaaa tgaactgaca tcttctttgg
     4921 gaataaccta cgaaaataat tttgataaaa ctaagggtag cactacctct caagtagata
     4981 gaaataccag tcgttttgcc atttcacctc aggcaactta tcagtttgat gttaatatta
     5041 gaggtggctt aaccggcagc tatgaagtaa attccgataa aaaaactgag gatggaactt
     5101 ctatttttag tttaggtgtt tgggtagaaa ttaatctata gccggaggat gccgctcctc
     5161 tggctacact aaaaaccgag ttctctcagt gctgattgct tttttctcca tttgggctta
     5221 attttaacaa ataaatgcac ttgaaccggc atttgcataa aacgggtaag ttctctttct
     5281 gcatattccc ttactttttt gatggtggag ccattttttc cgataagaat gggtttttgg
     5341 ctttctcttt ccagccaaat tacagcatca ataattacct tgtcggtaaa ttctttaaag
     5401 cgttcaatca gaacagctga agaatagggg atttcctctt caaaaaagtg gaagattgct
     5461 tctctgataa tttcctgagc aaaaaaacgc attggcaaat cggaaagcat atcattatcg
     5521 taatagggct cgtgaaaagg gatatagtat ttaattgctt ccatcaattc cggaatgttc
     5581 tctccggttt tagcagaaac aaaaaagacc tcatttatgg attcgggaag atattttacc
     5641 agctcgtttc tatctacttc gggattgagg tcaagtttat tgaaaacagc caattgagga
     5701 ttttttaaag ttttcagctg gttaagcact tctttgtcat attctgtggg aaaaccgtca
     5761 atttgggtta agagaataat caaatccaca tcttttaaag cattatgcca aattttaagc
     5821 atcttttctt gcagttcata gcggggtttc aaatagccgg gtgtatctac aaaaataatc
     5881 tgatgttcag aagtattcca tattccttta atagcataac gggtagtttg tggcttggga
     5941 gaggtaatag aaattttttc ccccaaaata aggttcatta aagttgattt accggtattg
     6001 ggtttaccga tgatagttac aaagccgctt ttgaaatttg ctgaattatt cataaattaa
     6061 aaaaaacaaa gggtggaaaa gccacccctt atagttattt attgacctaa gtttgcactt
     6121 aaatcattaa aaacatcttt gataatttga atacattcat ccaattgctc tttggtaata
     6181 atcaagggtg gagcaagacg aatgatatga cggtgagtcg gtttgcaaag caaaccttta
     6241 tctttcagtt ttaaacacac atcccatgct tcaaatccat tcattggttc aattacaatt
     6301 gcattcagaa gaccttttcc acgcacaagc ttgagcatag gatgttctat tttacgcagt
     6361 tcattgcgga aatattcacc caattcaaaa gcgcgttcag tcaatttctc ttctttaata
     6421 acttccaagg cagcaatagc tacaacacag gcaagaggaa aaccgccgta agtggaacca
     6481 tgttctccag gttttatttg cagcataata tccttatctg ccaaaacagc tgaaacaggt
     6541 aatacacctc cggaaagtgc tttacctaaa attaaaacat cgggtcgcac attttcataa
     6601 tcacaagcaa gaagttttcc ggttctgcct aaaccgcttt gaatttcatc ggctataaac
     6661 agcacattat attgatggca aaggtcataa caatctttta aatagccatc tttgggaaca
     6721 actacaccag cttcaccctg aattggttca acaataaaag cagctacatt tttgccgttt
     6781 ttttccagat actcttttaa agcattggca tcatcgtaag gaatacgcac aattccagga
     6841 gtgaatggac caaaaccgcc aaaacaatcc ggatcggaag aagcagaaat aatagatatc
     6901 gttctgccat gaaaattatt atctgcaaat ataagaatag ctgaatcgga agctactcct
     6961 tttacattat atgcccattt gcgggcaagt ttcattgccg tttcaactgc ttcagcacct
     7021 gaattcatag gtaaaaccat atcgtaacca aaatattcgg ttatatattt ttcatattct
     7081 cctagcttat tattgtaaaa agcgcgtgag gttaaagtaa gctcttttgc ctgatcaatc
     7141 aatgcctgaa taattttggg atgacaatgc ccctgattta ctgctgaata ggcagaaaga
     7201 aaatcgtaat aacgatttcc ttcagggtcc caaacataaa caccctctcc ttttgctaaa
     7261 accacgggca aaggatgata attatgagct ccatattttt cttccagttc cattgcttca
     7321 ctgctgctaa tgttgccgta tttcagattt tgagacattt ttcctccata atttttttcc
     7381 acttctacta tttcattatc ggttgagcag tcgcatttca gattcgtgga cattttttcc
     7441 tccatatatt ttattatttc tatttcttaa actcttattt ataaaggggt ttttgtgtca
     7501 aggggaactt ttcagccgtt tggttatatg atacacaaat gtgaacgaaa gaatggtagt
     7561 tatcacatca gcaataggta ttgcatagat caaaccgcta aattggaaaa gataattcag
     7621 caaatataac attggaatat agacaattcc ctgacgcgat aaggagacga tgaatgcagg
     7681 tattgccttt cccattgctt gaagtgcagc cattaatatc attcccaaac ccgcaaacgg
     7741 aatagcaata acataggcat tcagtatctg tgttccaatt ttcataattg ctttatcttt
     7801 gataaatacc gcaattagtt cttcagggaa tagagcaaaa aacaaggtaa agacagccga
     7861 cattcccaaa cagattatac tggacatctt aattactttt ttcatccgag tataattttg
     7921 tgctccataa ttaaaaccaa taagcggctg aataccaata gataagccaa taaaagcaaa
     7981 aataactatg gaaaaaaccc gggaaacaac tcccagggca gcaatggtat gatccgtgta
     8041 agcagaagca agcttaaaac ttatggcatt tccgatactc atcataatct ggcttaaaga
     8101 ggaaggaatt ccaattgcaa atatttcttt atagcaagac caacgcgggt gtaaatactt
     8161 tcttccggga ctggctaagc tcttcttttt taagtaataa gaaatataat aaagcatagc
     8221 taaaccctga ccaattactg tagctattgc agctccggtt acatccatct taaaagtaaa
     8281 aattaacaat ggatcaagaa taatgtttgc tcccgttcct ataaacaaac ccaacattgc
     8341 ttctttagct gaaccttcag ctctaagcag ttgaattaaa gtaaatttaa tcattatgat
     8401 cggactgcca ataaaaattg cagttaaata cttcctggca gaaagtgcag ttaaggcact
     8461 ggcaccagat aaattaagat aggtaggaat aaaaagaacg cccaaggtag cagcaaatat
     8521 tgatacaatg gctacggtaa aaatagcagt agtagtggtt tcccgagctg tttttagatc
     8581 ttttttccct aaaagacggg agagatagct ggctccaccg ttggcaaaaa ttcccgcaat
     8641 cgccattagc atattaaaat aaggcagagc aatagagaca ccggcaacct taaaagggtc
     8701 attcagcttg ccgataaaaa aagtatctgt taaattatat aagatattta ccagcatact
     8761 tagcatactg ggtagggcaa gataaagtat agaataggca acaggtgtct tttccagaat
     8821 ttcaatttta ctgtgtttca tttgatgttt tcctgataaa accccttttt taaataagca
     8881 gaattctgtc aagacaggaa gttattttca aatagaattg ctttagcatt gctcacccaa
     8941 tactaatgcc attagcaatg ggtaagcaat aggtaagcaa tgctattaag gaagaagatt
     9001 aaaggtagtt atcttatcct aaataataca gttaagaaaa gataggttgg caaattcttt
     9061 ctttgccaaa tagttcgcag atcttaaaaa aaattcttgc cttaccttgc ctacaaataa
     9121 taatggcaat tgaaaagata taagtatact cttattggtt gaaggtataa ttttagaaga
     9181 taaaaataaa taaaggagcg taaaatgaat tttctgaaac gaataggaat cttcctgctc
     9241 accaacattc tggtaatgtt ggtgttagga atcgtagtag gtcttctggg tttgaaaatg
     9301 gaagacctat ggggtttgct gattatatgt gctatttttg gaatgggggg ttcattaatt
     9361 tcgctgtttt taagcaagcc aatggcaaaa gcaagctata aaattcaaat tgtaaaaccg
     9421 ggaattgcgc atcccaaaat tgcttatttg ctttctgcaa tagaaagtat ggcagaagaa
     9481 agaaatatta aaatgccgga agttggtatt tataatagca gggatgccaa tgcttttgct
     9541 actggagctt cccaaaacaa tgctttgatt gctttttcct cggctttaat agaccgcctt
     9601 tcggaagagg aattagccgc tgtagcgggg catgaaatga ctcatatcac caatggagat
     9661 atggttacaa tgactttgct gatgggagtt gtgaatacct ttgtgatgtt ttttgccaga
     9721 attttaacgg tcgtaattga taatgcattg cgtgatgatc gcggtggcgg attaggttat
     9781 tttggatata taatggtgat tatggttctg caaaacattt taatgcttct tgccaatatt
     9841 cccattgcgg catattcgcg ttggcgtgaa tttaaagctg atgcgggttc ggcaaatttg
     9901 gtaggtgcag gtcctatgat tgaagcgttg aaaaaaatag ctaatgctta tgttccggaa
     9961 aaacgaagtg attcttatgc cttggcaaaa attaataatc agaaaaggat ttcccttttt
    10021 gctactcacc ctcctattga agtgcgcatt caacatcttc aggaaatgct ttgaaacaca
    10081 tactgaattt agaaattgaa aaaatggcaa tgggcggttt tggcattgct tatgcagaag
    10141 gaaaggcaat ttttgtcccc tatactgctg ttggagatgt tgtagatatt gatattacaa
    10201 tagaaaagaa ggattatgct tacgggaaag tgacaaaata tataagttat tcggattccc
    10261 gggttgaggt ccaatgctca tcctttgctg cagaaattcc ctgtggtggt tgtgattggc
    10321 tgatgttaaa atattctgaa cagctgaaac aaaaaaatca gttgcttctg gaactcttca
    10381 gtccttttat agataaggat gttatttact ttcctgctcc ttctacccaa ataaaaaatt
    10441 atcggaataa ggtctttatg ccagtaggga aaaattatca aacgggtaaa atcacttatg
    10501 gaatgtttgc ccgtttttcc catcaaatag tagaacacga tacttgtgaa aatcatcccc
    10561 cgatttttga tcagctggct aattccctaa tggaattttg ccttaaagcg aaggttgagc
    10621 cctttaacga aattgaacac aaaggtcaat taagacatat cggttttcgc tgtaattctg
    10681 atagcagaca aattttggtg atccttgtta ctttaagcgg aaaactgcct tttaccaatc
    10741 ttctggtgaa aaagctgact accgaatttc cccaaataaa aggtattatc cagaatatca
    10801 atcgccaaaa aggaaatgtt attttaggta aagaaaccaa gctactctac ggaagcgaga
    10861 atttatacga taatttatgc ggtttgaaat tcaggattaa ctaccgcagt ttctggcaaa
    10921 taaataccgg aacaatggaa atgattattt ccttcctgca ggattttgtt aatagtaacg
    10981 atgttattct ggatgcctac tgcggaattg gagctatagg actttcttta gcagataagg
    11041 ttaaacgcgt tattctgctg gaagaatttc ctcaggcaat tgaagatgca aaagaaaatg
    11101 cagttctgaa cgggctaaac aatgtttctt ttgtcgtagg caaagtggaa gataacctgt
    11161 ctgctgtttt aataaaggaa aagcccgata caattataat ggaccctccc cgcagcggag
    11221 ttcaacaaaa cgctttggaa gcaattatac aagcggaagt taaacaaatt atttacttaa
    11281 gctgtgctcc tatgactctt gcccgagatt taaatacact aattggcggg gagaaatata
    11341 aagttgtctt tttacaaccc tttgatatgt ttcccaatac ctggcatata gaatgtcttg
    11401 ccgttcttaa atataacgat tataatataa atatgagata ataaaataat tggttaggaa
    11461 aagaatgaaa agatacttat atctaatctt cggtatttta ttgatgctgg ctttaggaag
    11521 ctgcgaaagc aatgacagcg atacaattgc ccagcgtgaa attcgggata ttctctatga
    11581 tataagtatg gaattcaact tgaaaaatat aagtggaata atggaacatc tacatattaa
    11641 atatctgcat aatggaatga caagttataa ttttaatgat ctatggctgg atagaatggc
    11701 tcaatactcc ctgctggaaa tagaagttct ctatattgat ttggatgacg actatgctgt
    11761 tgtacatagt aaaaataaat ttacttccca tgcggaaacc gttactttaa atgaaccgga
    11821 ggactctgga gatatttcct atttcaaaag agaaaatggt gtttggtata tctacggtaa
    11881 ccaacgccta tattaatgaa ctctcacaga tttaatagat aagacctttg aataattggt
    11941 atatgaaagc tatgttaata taattcaaag ctactcccct ttgtagtttg ataaagcgta
    12001 tttaactaaa tataaaaatt atgttttatc ccaaaataac tattggacca agatgaaaaa
    12061 gttcctgcag aacttttttt attctcctct tattcgccgg tttgaacccc ttctataata
    12121 gctgtcggga cagccccata tccggatttt ttatagaata aattcagtat gcattattca
    12181 tttttatgtt caacgatctt ataatttatt aaagcggaca tcaaccttcc ggattttgtg
    12241 tttgaaggca tcaacgattc tgtctgatcg cttttacagc taaatagcta tatttatcgt
    12301 tacctattcc acaaagttta tccagaaaat tccataccct aaaaggaact ttttgcagtt
    12361 ttttatccag acctaaagca gaaagcaatt tgtgagcaaa caaagttgct cttaagatat
    12421 agaatccatt attctccaga atagatgcca atttaggtgc atcccaacta tgcaaatgcg
    12481 cattcatcgg tgttttgcga ttacaatgaa tgcaaagaga atagcggata acttctttgt
    12541 agggaactgt tacaaaaaga attccacctg tctttaacat ctttttccag ttgtctattg
    12601 ccaaagctgg atcaacaagg tgttcaagca cttccagggc aacgattaga tcaaatgatt
    12661 ttcctggtag atttattgaa caagcatcac cataaatggt ttttatgcgg ggatcaatat
    12721 ttttaatcct gtttagattt tcttcgctaa ggtcaatagc agttacttct gcttccatat
    12781 ttgcacaatg caaagaaaac caacctctac cactgccaat atccaagacc ttttgcccct
    12841 ttttaattgg acacagatgt tgaataaatt gatttcttct tatttctgca ggagttatta
    12901 agtccttacc ccaataatca aattccaggg catcattatg ataatgctga atgtaatcca
    12961 tatttaatcc acaattttgt tttccctttt tataaactaa gcgaaaaaga atatccggca
    13021 aagttatttc ccgcaggtgt tgtttcccgc agatttcgca gataaaaacg cagatttatt
    13081 tttaagttct tagttctgag ttcgtagttc ttagttctcg ggaagtaagt attatttttc
    13141 cctttatctt atttctctct ttttctcttt ttctctttgt gaaaaattac tcttctcact
    13201 ttctctcttt ttctctttct ctctttgtaa aaaattactc ttcacacttt ttctcttttt
    13261 ttctttttct ctttctctct ttgtaaaaaa ttactcttct cactttctct ctttttctct
    13321 ttctctcttt ctctctttgt aaaaaataaa tccatcccgt catctcgcaa aaaaaagatt
    13381 tgacagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctc tttgtaaaaa
    13501 ataaatccat cccgtcatct cgcaaaaaaa agatttgaca gtttctccgt cctaaaattt
    13561 ggtggcttaa attattcatt acgctcatag cgtaaagcac aagtcccata ttttgcaggg
    13621 gcttatagtt ctttaggagg aacaatgtca tccattaaat gtggtactca cattgagtat
    13681 cgctgtggaa ggggagttaa cccaatccac cacttaacta aaacaaaccg cagtttgatt
    13741 tcctgcacag atggcggagc tctacaaaag tttcgtttta acccagggga aaccattttg
    13801 cataaatttt ggaggttcca gtgagtaaac acgataaccg aatccgaata aaattgaaag
    13861 cttatgatca tcgtcttctt gataagtcgg tggctgaaat cgtgaaatcc actcgcaata
    13921 cgggagccaa agttgtcggt cctattccgt tacctacaga taggactatt tatacgattt
    13981 tgcgtagtcc ccatgccgac aaaaagagtc aagatcagtt tcagatgctg gttcacaagc
    14041 gtttaattga tattgtgaat cctactcagc aaactactaa tgcacttaag aaacttagct
    14101 tgcctgcagg tgtccatgtg gaaatcaaag cgaacacaag gagctaagga tgattggatt
    14161 aattggtaaa aaaataggta tgacacaggt gtttgacagc gatggtaaag taattcctgt
    14221 taccgttatt aaagccggac cctgtaaagt tatctgcaaa agaactgaag ctgaaaatgg
    14281 ctatgaggca atccaggttg gttatgaaga aattcctgcc cggaaagtaa gtaaaccatt
    14341 gcagggtcac tttaaaaaat acaacagtcc tttttaccgt tatgtgcgtg aatttcgtcc
    14401 ttcttacgga caacatattg aaagtgttca aattggggat gaactgaaag cagatatttt
    14461 tggagaaaat gaaacggttt ctgtttctgc caattccaaa ggacgcggtt ttacgggagt
    14521 gatgaaaagg cataatttta atggttttat tagttctcat ggcagtcatg agtcctttcg
    14581 gggacctggt tctataggtc aatgtgctca gccctcgcgt gtttttaaag gaatgaaaat
    14641 ggctggacaa tatggtaaca cgaaagttac tgtgcatcat ctcaaggtta taaaagttga
    14701 tcctgaaaat aatataataa tggttaaagg tgctgttccc ggtcatcgta attccttagt
    14761 aacaattcac aaggaacaat aggtggtaac ttatgttaac agcaaaacga tataatatgc
    14821 aaggtgaact tttaggagaa gttgatcttc ctgaaggcat ctttgaagtg gatgttaatg
    14881 ctcctaagat tttactcaat gaagtggtta caatgttcct tgccaatcag cgtcaaggca
    14941 cagtgcaaaa gaaaaataga agtatgactg ctggaagcac gcgtaaactt ttccggcaaa
    15001 aaggaacagg taatgccaga gtgggaactc gtagaagtcc tattcgggtg catgggggaa
    15061 aagcgtttgc tatttatccc aaagattggt atcgtaaaat tccccgcacc aaaaaacgcc
    15121 tcgctttgaa agttgcttta acagaccgtg ctcgcgaagg cagaataatg attgtagata
    15181 atctaagttt tgaaaaaccg aatactaaac ttgccgtgga acttttaaac aaaattatac
    15241 cggaaaaagg ttataaactg attgtaactg atggtcacca tattccaact gttaaaagtt
    15301 ttgctaattt gcctgaggtt atgacggaca gagcagatag tttgaatgct tatgaagtgc
    15361 ttaaaagcag ttatataatt atgactgatg aagctctgaa aaaagtggag gaggtattca
    15421 acaaatgata cacccacgca atatcataat tagcccgata atcactgaaa aaagcagtaa
    15481 ccaggttcaa aatgaaaata cttacagctt taaagtgagc agtaatgcta ataaaataga
    15541 aatcaaaaaa gctattgaac gcatttttgc tgttaaagtt ttggcagtga atactattcg
    15601 ctataaaggg aaacccaaaa gcttaggtaa atataacggc aaaaggcctg actggaaaaa
    15661 ggcaatagta accctgcgcg aaggtgataa gattgccgat tttgaggttt aatgaggtga
    15721 ataatgggaa ttaaaaaata cagaccaaca acttcctccc tccgttttcg caccggtcat
    15781 acttttgagg agataactaa aaagaccccc gaaaagtcat tactgaaacc gaaacataga
    15841 accggaggca gaaacaattc cggaagaata acctgtcgtc atagaggtgg aggtcacaga
    15901 cgccactatc ggataattga ttttaaacgc gataaaattg gaattccggc aaaagttgct
    15961 gccatagaat atgatcccaa tcggacagcc cgtattgctt tactgcatta tgtagatggc
    16021 gaaaaaagat atattgttgc tcccgatggt ttggaagtag gagctaaagt gatgagcggt
    16081 cccgatgccg atattgcagt gggaaacgct attcctttgg aaaagatacc cttgggaagc
    16141 gttgttcata atgtagaatt gaaaagagga aaaggtggtc agatagctcg tagcgctgga
    16201 acttttgctc aggttgtagc taaagatggt gattatgttc atattaaaat gccttccaat
    16261 gatgtgcatt taatccggaa agaatgtctt gctaccatag gaagcgttag caatccggat
    16321 cattctttaa ttcaaattgg caaagccgga cgtaaacgct ggatgggaat tcgtccttca
    16381 gttcgtggtg tagcaatgaa tcctgtagat catcctatgg gtggaggtga aggaaaaacc
    16441 tccggaggtg gtcatcctgt ttccccttgg ggtaaacctg caaagggtgg aaaaacacgg
    16501 aaaacccata aatacagcga taaatatatc gtgaaagcag ttaagaaata gaataaggat
    16561 agagaaaatg gcacgttcaa ttaaaaaagg tcccttcgtt gatgctcatc tgctgaagaa
    16621 agtagaagtg ctaaatgctg aaggtaagaa aagcgtaatt aagacctggt ctcgccgttc
    16681 tacaataact cctcaattta ttggtcatac tttcagtatt cataacggac ataaatttgt
    16741 tcctgtttat attactgaaa atatggtagg tcacaaattg ggtgaatttt ctccaacccg
    16801 tacctacaga ggccataaag aaaagaagaa aaaagttacg agatagggag agagaaatgg
    16861 aagcgaaagc aaaactttgc tatgctcgcg gctcagcaag aaaggcacga ttagtgttag
    16921 atacaattcg ctataaacct gtaacagaag cacaaaatat attgcgtttt tcacgccgta
    16981 gagtagcagg aacgataaat aaattattgg cttctgccat agccaatgct caacatcagg
    17041 atcctaaagt taatctggat aaaatgtatg taataactgc cacagctgat gagggtccgc
    17101 aaatgaaacg ttatatgccc cgagccatgg gaagagctta tatgattagg aaaccgacct
    17161 gtcatatctc cctggaaata caaacaatag aagaagaata ggaggaaaac cttgggacaa
    17221 aaaatacatc ccgttcttta ccgtctcggt gttaataaag aaacagaatc catctggttt
    17281 gctcaaggaa cctcgtatgt ggattttctt caggaagata ttaaaatccg gaattatatt
    17341 cgcaaacgct tgagtgacaa gatggtttcc cgtgtgcaga tttcgcgtaa aacaagttca
    17401 ataataattg atatttacac tgcccgtcca ggtttagtga taggcaagaa aggtgaagat
    17461 attgataagc tgcgtaatga actgaatgtt tttattaaca aagaccgaaa aaatccgatc
    17521 agtgtgtcta tcaacattga accgatagat aaaatgtggt tggatgctcg tttggtggca
    17581 caagaaattg cgcgccaact ggaagagcga atttctttcc gcagggctat gaaaatggct
    17641 attcgttatg ttatgaaaga aaacgctcag ggtgttaaag ttcaagtttc cggtcgcttg
    17701 ggtggagctg aaatagcgcg ctcagaaaga tataaacaag gcagaacccc gcttcatact
    17761 cttcgtgccg atattgatta tgccaatgtg gaggcaaata ctacttatgg tgttatcggt
    17821 gttaaggttt ggatttataa gggtgatata cttgattaaa ggagatatgt aatgttagca
    17881 cctaaaaaag taaaatatcg caaaatgatg aaaggaagaa ggaaaggtct atcctggacc
    17941 ggatgcaaca tctcctttgg tgattatgga ttaatagccc ttgaggactc atttattact
    18001 agccgccaaa ttgaagcatg ccgtattgcc attactcgtc atatgaaaag agaaggaaag
    18061 gtctggatta gaatatttcc tgataagccc attaccaaaa aacctgccga aacgagaatg
    18121 ggaaaaggta aaggcgctcc agaatattgg gtagcagtag ttcgtccggg aagggttctt
    18181 tttgaaattg aaggtgtgga tgtgaatgtt gctaaagaag cattgcgttt agcagcgcac
    18241 aaacttccga ttaagacacg ctttattgct cgcgaaggag tagaattatg aaaatagagg
    18301 aaattcgcga tttaagcatt gaagaattgc aggcaaaact ggaagaatta cgcatagagc
    18361 tttttaacct gcgttttcaa aagacaaaaa acctgctgga ccgtccagac cgtattcgta
    18421 atatccgaca tgatatagct cggattaata ctattttaac agaaagagaa aaagagaaaa
    18481 aaatttgagg tgataagtgg caacaacaca taaaatgatt aaacagggaa ttgtcgtaag
    18541 tgataaaaat gataaaacca tcgtggtccg cgtagaacgt cagtttatcc atccgttata
    18601 taaaaaaact gtgcgccgac ataaaaaatt tatggcacac gatgaaaata actctgccca
    18661 cgaaggtgat attgttcaaa ttatggaatc acgtccattg agtgcacgca aacgctggac
    18721 tctgcataaa attgtggaaa gaagcaaata aaggagtttg gggaagatga ttcaggttca
    18781 aacaatgtta aatattgctg ataactccgg tgccaagaaa gctatgtgca taaaagttct
    18841 tggcggaaca caacgcaaat atgcttcaat tggagatgtt atagttgtag caattaagag
    18901 tgctactccg ggtggaaaag taaaaaaagg tagcgtagaa agagctgtaa tagttcgcac
    18961 tcgcaaagaa ttgcgtcgtc cagatggttc ttatattcgt tttgccgata atgcagctgt
    19021 tattattgat gaaaaacatg aaccgaaagg aacgcgtatt tttggtcctg tagcccgtga
    19081 attgcgtgaa gcacattata tgaaaatagt atctctggct ccggaagttc tgtaggagga
    19141 aaggtggcaa agaataaaat gcgttttaaa aaaggtgatc tcgttaaagt tattgcgggt
    19201 gatgaaaaag gtaggaaagg tcatattttg aaggtctttc ccaaaaccgg aaaggttatc
    19261 gtggaaaaag tgcatatgat taaaaagcat gccaaaccca cacagcaaaa tcctcaaggt
    19321 ggtattataa ctatgga
//
LOCUS       DAOPFL010000027        19212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_3105_length_19212_cov_104.786710, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000027 DAOPFL010000000
VERSION     DAOPFL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 19212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19212
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3105_length_19212_cov_104.786710"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /gene="serS"
                     /locus_tag="PLG69_03415"
     CDS             <1..869
                     /gene="serS"
                     /locus_tag="PLG69_03415"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPI42589.1"
                     /translation="FSFTPKDHLELAIQNKLLDLPRGAKISGSGFPLYTGEGARIERA
                     LINFMLEFHLQKHHYTELMAPVIVNRKTMTGTGQLPKLEEDMYRIEKDDLFLIPTAEV
                     PVTNIFADEVLSYKDLPQKFVAYTPCFRREAGSYGKETKGLQRLHQFNKVELVHFTEP
                     EKSAEALEQILSDAEDILQAFNLHYRVAALCTGDLSFASQKTYDLEVWAPGTGKYLEV
                     SSVSTFGEFQARRANIRYKDKTGKVKHLHTLNGSGVATPRLLIAIWETYQQEDGTIKL
                     PAVLDPFLGITI"
     gene            942..2129
                     /locus_tag="PLG69_03420"
     CDS             942..2129
                     /locus_tag="PLG69_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPI42590.1"
                     /translation="MNKNELGLLDIILVIAKRKTLVISICLLVAIGAIIYSLVVPQYW
                     ESKATLMPIAESGSLGSLGGGIMDILGSGLLQTDKYDMAVDFIYIMQSRKFQEEVIRK
                     FNLIPYYKINEPDTLKAMELAVKKLSESTMQIFYDQKTYLVNIIAETKNKELSRQIVQ
                     YHLDSLNKYILHSKMSKGRQKREFLEKQVDSHLQAVYSLSEQIRDFQKKNRSIDITQQ
                     TEAQLELYSDIVAEYMQTEIEHSLALSQYSADSPVVINLAEKLQLLKKKIKSLEKSGS
                     DLVPEYIVQIDKIPDLAMQYAQMMLNLEIEKKIIEYLYPQFELAKLEEVKDLPTFEVY
                     DAPQLAGIRSKPKRALTVVISTVAAFILSCLLALIIESLQGENKEKTQAIKAALFGRK
                     KAS"
     gene            2246..3406
                     /locus_tag="PLG69_03425"
     CDS             2246..3406
                     /locus_tag="PLG69_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPI42591.1"
                     /translation="MYFLFVACLCIIYALINPDLIVINKINFLPAMFMLAWTSYSFLS
                     YNWSFVKQVSVVHSILILKKAMLFIIVSIFCRHQTVKKYAPWFWFSVILVYIVIALWE
                     MLTWQHLPQSLYYNKTNFVPTGPFYGPNQLAAAFNLLLPFILFLPEIKKSKFWGVISL
                     LCFFTIMGIIVIEGARIAIIACSCLFLFAFIFLYSARMRKLSLFLMALIIVGLVIFAK
                     PLVSFGTEMFTEEISSIGQESKSFTMSSIKIRTRLIPQNLEILAESGFKGVGAGNVEH
                     YMLAGRMYKTGGISNSHNFFLELLTNYGIVFFLGFAYLYVLWLYRLWRAIKMNPENRH
                     YYEMYFFALLMFLPTSILPSSIIWEHHYWILFAIINEISHPDNWMPRQYEQI"
     gene            3393..4160
                     /gene="mazG"
                     /locus_tag="PLG69_03430"
     CDS             3393..4160
                     /gene="mazG"
                     /locus_tag="PLG69_03430"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPI42592.1"
                     /translation="MNKFEQLVEIVATLRTPAKGCPWDLKQTRESLVPNFIEELYEVV
                     EAIENKDYDALKEELGDLMLHIVMQAQISKEQGLWNIDDVLEEISSKLIRRHPHIFGD
                     LHLTDADEVKQNWERLKKAEKTERKSVLDGIPKSLPALIQAQRTQEKAASVGFDWQDI
                     KPILEKLDEEREELAEALNSNEQDAIKEELGDMIFTLVNLARKLHIDAESALKECIRK
                     FTRRFNAIEEHYLKNGEDINEAGLEELDSHWERTKKH"
     gene            4105..5658
                     /locus_tag="PLG69_03435"
     CDS             4105..5658
                     /locus_tag="PLG69_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPI42593.1"
                     /translation="MKPVLKNSILTGKEPKSTNLFNRLRFILIFGSLAIFIFFAVYIQ
                     FLLKQAKKEQEFIPRIFAQYIAYTDRYLREAERNAQLITEISSKYLQFTTSRDFKQDL
                     WDYISTEFMQENPVPVIITDQNFQPLLWKNVGVPTDTIFHELSPENQKKLENLMKTMV
                     QMPLTDDGKISGYAYYSKPVSFAEFIKNVDCSIIVTDHYKQPLFWRNVGIPETANYYE
                     ISTEEQQKLAHQIKTMDEIPLSNAADSLGYIYFSTPKSLSYIRYIVILELILALMVVF
                     FGIYGLFLLSRTEKDTLWVSLAKETAHQFGTPITSLMGWLDYLKEPSPDGCVRDIDQI
                     VQYMTADLEHLKTIASRFGKVGSITKLEPQNLDRIISETVEYFRERMPHLGHKINIHY
                     ISKIEGVMVLMDKDLIKWSLENLIKNCVDAMSQKGGNIIITATHKNPWIYIHIRDEGK
                     GIPHRQWKRIFEPGVTTKTRGWGLGLSLAKRIIEEYHNGHIKVLQSTIGEGTTFEIKL
                     PVQETNKEK"
     gene            5658..7208
                     /locus_tag="PLG69_03440"
     CDS             5658..7208
                     /locus_tag="PLG69_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPI42594.1"
                     /translation="MAYVFTPSQMKTLDENTINCFGIPASILMENAGRGCADFLINNY
                     PEESKGVIIILHGYGNNSGDGFVLARWLQNYGKQVILLQVQKGEMTEETRHNFKLCKA
                     LKMPTFELNKEYDNLDILENVPGVTMIIDAVFGTGFKGKLPASLKKIFQSVDDTSALK
                     VAIDIPSGLNGATGEGEDAFRADVTLTLHSYKMGFFLREGKIKCGKVYTIPIGIPEHY
                     NDAFFTPAIFIDNKTFRLPERYYYANKSNYGRVFIIGGSYGLLGAAALATRAALRAGA
                     GLVYLISREENKYFYNANPAEFLFIGIPRIEGTLTPDLKLFTTLFKTADSVVIGPGLG
                     SDNYGTYMLEYILRTSTVPTVVDADALRIIAKDPSLQQYLKKPNILLTPHLGEFCTLT
                     DMDMDKLVKDIPTALENYIKKTGAKVLLKSDTTIFSDGKRMFINISGNDGLATGGSGD
                     VLAGIIGSFCGQKMELGEAAINASFIMGKTAEELAKQRYFPSILPTDIIENLFMPLKN
                     ESLSSPEG"
     gene            7521..8447
                     /gene="lepB"
                     /locus_tag="PLG69_03445"
     CDS             7521..8447
                     /gene="lepB"
                     /locus_tag="PLG69_03445"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPI42595.1"
                     /translation="MKEKITPKRDKTKSDNLPYTLTPPPEGKKFHKRKPGLQDWMEAI
                     LFAFVVAMIIRNYTFENFLIPSSSMEKTLLVGDYLIANKIKYFFNDPKQGEIVTFRYP
                     KIEEGTPEHKDYKDDFIKIFPPIYINKRHSFAKHPFTAFHICYYARRNVVKRVIGMPG
                     DIVEIKDKIVYVNGKEYTGGFENYGVAIPSPPAAPERIEYFTYPENSFDYVSMNPWYE
                     PYRVKIEGDSSAVRKIFNRDWFGPVKVPEKSYFVMGDNRDVSEDSRYWGFLERKYITG
                     TPWLIFFSRGIEFNKLYDEPHIRWNRIFRHPR"
     gene            8417..9541
                     /gene="hemW"
                     /locus_tag="PLG69_03450"
     CDS             8417..9541
                     /gene="hemW"
                     /locus_tag="PLG69_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPI42596.1"
                     /translation="MEQDFPSSPLGLYIHIPFCLSRCGYCSFFSVPYRNSVLNEYVDY
                     LEKEKSLYSNQLNRPLATIYFGGGTPSLLNAEQIRHILSGLNILPEAEITLEINPLQI
                     TQTYLQALKTTPINRLSIGLQSMNQEELNFLERHHKPEQIQDKIALCRQFGYENLSLD
                     LIYGLPNSSLQTLQGTVEKYLELKPEHISCYLLTLDADCKLAKEKNNLPNEEIQAEQY
                     AFLCNYLEKEGYIHYEISNFALPGYTSQHNLRYWHNDDYLALGASSAGWISPIRYQNP
                     ADLNLYYQSIEKGETFPQKEELSQQRIIQDYLMMGLRLKEGIDLDEFYNRFQVKLTEL
                     YGERIFKLQQIGMLNLTEHNLALTEKALFVSNSVIGDLIL"
     gene            9538..10251
                     /locus_tag="PLG69_03455"
     CDS             9538..10251
                     /locus_tag="PLG69_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPI42597.1"
                     /translation="MNLKELCLFLRELIQDSEFADKSYFAGGCVRDFILNPDATLKGD
                     VDICVELPEGGIALAQYLQNRLQASELVIHKSFGTASLLYKNLQLEFVATRKEQYFPN
                     NRYPKVEFGTLVDDVLRRDFTINALLMAIATGEILDLCQKGLNDLQQGIIRTVKDPNL
                     SFMEDPLRMLRALQFAMRFNFTIEENTFKAMQKNAPALKILSHRAIQNELQKIPESQK
                     SKAKQLISKLGWEIILSLF"
     gene            complement(10393..11820)
                     /locus_tag="PLG69_03460"
     CDS             complement(10393..11820)
                     /locus_tag="PLG69_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPI42598.1"
                     /translation="MHTKTVISGIRWTLSTTILRRAISLILFIFLAKWLTQTDFGIYR
                     SFSAILALLTYVTHLGLDYVYLVSRQKERVNFLALLQTGLLVSAIITVLLAVFGKNIG
                     AYYHSAELGKVLSYGGGLIFIESLRRIVRVYAQKKFQFKDLALAETINVIIYSVLCLA
                     LIYFWRYAWLFILLFYIGNLAELVYLCVKLPRLPLSLLKRVFSFKWLKKTLALFSRNS
                     NFLLMVNAINLLQAYSINAPILFLGTLVEPALMGVYFFASQLIAIPVGMLNASFAQVF
                     FPVLAQSEKSASVAGIRHYTSLVLKIGIPALIVYALLLQYIVPVIWGDKWLAAMPLIL
                     YLVIYYGTSMLHNPISGIPFICRKPQWELLWNIITLGLRILVLYLGLQVSFAFAILLF
                     CLVSAVMHFAFYYMSLALLKADLWQITIDILTYLPVLLVLALGCLYMGKFSIVFPLVV
                     LIGYGIYLWIAERDTLKEVLSLIKK"
     gene            complement(11830..12846)
                     /gene="ruvB"
                     /locus_tag="PLG69_03465"
     CDS             complement(11830..12846)
                     /gene="ruvB"
                     /locus_tag="PLG69_03465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPI42599.1"
                     /translation="MLERINNPDKLNEDVELDRALRPRTLKEFIGQTHIKELLDISIK
                     AAKLRGESLDHILLYGPPGLGKTTLAGIIAREMGVNITVSSGPVIEKPSDLAGILTNL
                     QRHETLFIDEIHRLSHIIEEYIYPAMEDFEMEIILDSGPNSRTLKIPLEHFTLVGATT
                     RAGLLTPPLRDRFGIILRLDYYDQESIAQIIRRSARLLNIPTEDDGVQEIARRSRGTP
                     RIANRLLRRVRDYAQIKGEGIITLDIALSALQMLQVDNAGLDEMDKRILTTIIENYNG
                     GPVGIKTIATAIGEDSGTIEEIFEPYLVQQGFLERSPQGRKVTFKAYKHLGLNPASAQ
                     TEIF"
     gene            complement(12866..13582)
                     /locus_tag="PLG69_03470"
     CDS             complement(12866..13582)
                     /locus_tag="PLG69_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPI42600.1"
                     /translation="MKTLIIIPTYNEIDNIEKLLEQVLAKSETIEVLVIDDNSPDGTA
                     LRVKFMQSSEPRIHILERPGKMGLGSAYVTGFKYALERDYDYIIEMDADFSHNPEDIP
                     LLLNAAKKYDLVIGSRYCEGVNIIHWPIKRLLISYFASKYVRTITRMPIKDPTSGFKC
                     FQRKVLENIDLDKILSDGYAFQIEMNFRAWVKGFHIKEIPIVFTERKNGVSKMSRRIV
                     WEAAWMVWRLEFMRILGLLK"
     gene            complement(13579..14334)
                     /locus_tag="PLG69_03475"
     CDS             complement(13579..14334)
                     /locus_tag="PLG69_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPI42601.1"
                     /translation="MNRFIDESAKIGKNVTLGNNVVIMAGVQIGNDCLIGHNVIIHPD
                     TKIGNACRIDDGTIIGKKPLSSPRSIFTVPTDLKGTEIGDFCQIGSNVIIYCQCTIGN
                     RNLIADLATIRENVTLGDLNIVGRNVTIENFVHIGNRNKLETNCYVTAYSEIGDYCFI
                     APCVATSNDNYMGRDKERFKHFKGVTMMTGSRIGVNATILPGKTIHSDGTVAGGAVVT
                     KDVPAKTIVAGNPAKPFSEVPEPQLLENNLDKQ"
     gene            14406..15302
                     /locus_tag="PLG69_03480"
     CDS             14406..15302
                     /locus_tag="PLG69_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPI42602.1"
                     /translation="MEKYIADFCNYLALEGKSPRTITAYKLDLEQFHSFIQRYFENGE
                     VDIKGITVLNIRDFFRFLNEKPDCNRSLARKSAALNSFFRYCKRSGFIQNNPMEKIKR
                     PKYEVPLPKCFTEEEVRTLLSIPDTDSPFGIRNKAILETLYSSGLRISELAGIRLQDI
                     DLKRGLIRVTGKGNKQRIVPLGSYAIEAINNYLKVRPQFMRENNPDLLFLTKSGKAFD
                     TKQLDIILKRYFELVAKAKGYSPHSLRHSFATHLLSRGADLRAIQELLGHSLLSTTET
                     YTHISLEDIKEAYKKGHPRSKE"
     gene            15595..16977
                     /locus_tag="PLG69_03485"
     CDS             15595..16977
                     /locus_tag="PLG69_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HPI42603.1"
                     /translation="MKKILLTAINSSWSQSNPAIYYLREMIADLPYAVILHEWTLKDR
                     LWNVLSEIYCQKAEVICFSAYIWNRLYLQELIPEVIKLLPEAVIVIGGPEAESLSCLK
                     RKGLYIVQGAGEGKFRFLAEHDFSANDEDLSRAKHIPLKDIPFPYRESDGEILKGKLL
                     YYETFRGCPFACIYCLSANDKRSELRYQPNNQKDMQCLIKELETLISFQPRTIKFVDR
                     SFNLHKQLAHYIWKFFMKQNCPCDVHFEIYPDLLEEDDIALLEKAPENRIRLEIGIQT
                     INSEVALNCGRKSNWEKTKWTLQELKKRTNIRIHTDLLAGLPGENYASVLNGLNEVCA
                     TLPDAVQLGILKILPDTPMQKIASELNYKWLSQPPYQCLSSDALSFEEIQQLENFAKL
                     LNLYWNKEEHKSMWQEMLQTQSATDILTALQQKHQELGYELHSLSKAKRNAVIADICV
                     AGGRRPSAKK"
     gene            complement(17135..>19212)
                     /locus_tag="PLG69_03490"
     CDS             complement(17135..>19212)
                     /locus_tag="PLG69_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPI42604.1"
                     /translation="PFDSIIAAISQVPEVDIFTQENNYIADKILPVSRWQTAIVDEST
                     MFTGLGNVFAGGDFRRGAATAIEAIADGRIAATAINRFLETGEIAAERMLFDSKKANT
                     LQEVSPEEYSIYEKIERKEMPELPLIEAKNSFKEVELGFSEDDAVSEARRCLECGCFV
                     NETCFLRKYCTDYVVNPAQFLGDKNKHPIDYSHPFIMRDANKCINCGRCIRTCAEIQG
                     PAVLGYIYRGFATLVAPEFGDSLTKTSCLSCGKCIDVCPVGALMQRTVHYKFNPHPKD
                     ISLQNCGLCGVGCFIQTETQAGIVTRVTTPQEEPAFNGKNLCFKGRFGWQSFYDKDRL
                     KVPLFRENGIFKEIGWQEAIDLIAKEISLCSSKRFEISPYISLEEMLMLQRAAHKYET
                     ELSANPVFSAFSDVFLNLSPQKEPYKILDDFEEYVVYGELNQVLATLIRLQQRKGKKL
                     TLVNYPENAFCRFADAVYAKLAEVQATDKTLFIYNQNRISEQEAFALWCFASEQGTDN
                     LLVSTDFRNHLGFLALQPDFSPSSSDFVLSWGVYPALANQAKFSVAIMPFEDEKAPVD
                     LLIPAPSFLEMEGTALSDLGQITKSANPAKSIIINELMRLFYTLNWIHPNSAEVPFWN
                     QEAEKLLLTLQNYVPAKFEPSAVKIAELSKTIKFIPAQAEKHITALFEQGKKATSFSG
                     RLISSSTNM"
BASE COUNT         6134 a         3803 c         3581 g         5694 t
ORIGIN      
        1 aattttcttt tactcccaaa gaccatctgg aactggctat ccaaaataaa ctgcttgatt
       61 taccccgggg agcaaaaatc agtggtagtg gttttcctct ttatacaggt gaaggtgctc
      121 gcatagaacg cgctctcata aattttatgc tggaatttca tctgcagaaa catcattaca
      181 ctgaactaat ggctcccgta attgtaaacc ggaaaacgat gacgggaaca ggacaacttc
      241 ccaaacttga agaagatatg tataggattg aaaaggatga tttgtttctg attcccactg
      301 cagaagttcc ggttaccaat attttcgccg acgaagtgct ttcctacaaa gacctgcccc
      361 aaaaatttgt agcatacact ccttgcttta gaagagaagc cggttcctac ggaaaagaaa
      421 caaaaggttt gcaacgccta catcaattca ataaagtgga attagttcat tttacagaac
      481 cggaaaaatc tgccgaggca ctggaacaaa ttctctccga tgcggaagat attttacagg
      541 cattcaattt gcattataga gttgcagctc tttgtaccgg tgatttatct ttcgcctctc
      601 aaaaaacata cgatttagag gtttgggctc ctggaacagg aaaatacctg gaagtgagtt
      661 cagttagcac ctttggtgaa tttcaagcac gccgcgctaa tatccgttat aaggataaaa
      721 ctggaaaagt gaaacaccta catactttaa acggttccgg agttgctact ccgcgtttgc
      781 tgattgccat ttgggaaaca tatcaacaag aagatggaac tattaaactg ccagctgttt
      841 tggacccctt tcttggtata acaatttagt ttttaagaga attatgaacc tttccgagca
      901 ggagagaaaa aaaattttaa gacctcataa gaggatggaa aatgaataaa aatgaattag
      961 gactgctgga tattattctg gtaattgcca aacgcaaaac tttggtaata agtatttgcc
     1021 tccttgtagc tattggcgct atcatctatt cactggtagt tcctcaatat tgggaatcca
     1081 aagcaaccct gatgcctatt gcggaatcgg gaagtttagg ttctttagga ggaggcatta
     1141 tggacatttt aggcagtggt cttctccaaa ccgataaata tgatatggca gtggatttca
     1201 tttacataat gcaaagccgt aaatttcagg aagaggtgat tagaaagttt aacctgatac
     1261 cctattataa aataaacgaa cctgataccc tcaaagctat ggagctggct gtaaaaaaat
     1321 taagcgaatc cacaatgcag atattttacg accagaaaac atacctggta aatatcatag
     1381 ccgaaaccaa aaataaagaa ctctcccgtc aaatcgttca atatcatttg gattctttga
     1441 ataaatacat tctgcacagt aaaatgagca aaggacgcca aaaacgggaa tttctggaaa
     1501 aacaggtgga tagccatctg caagcagttt attctctttc cgaacaaata agagatttcc
     1561 aaaagaaaaa tcgctctatt gatattactc aacaaacgga agcccaactg gaactctata
     1621 gtgatattgt ggcagaatat atgcaaacgg aaattgaaca ttctcttgcg ttatcccaat
     1681 actctgcaga ttccccggta gttattaatc tggctgaaaa actgcagtta ttgaagaaaa
     1741 aaataaaaag tttggaaaaa agcggaagtg acctcgtgcc ggaatatatc gtccaaatag
     1801 ataaaatacc tgatttggca atgcaatatg cccaaatgat gctgaacctg gaaatagaaa
     1861 agaaaataat tgaatacctt taccctcaat ttgagcttgc caaactggaa gaagtgaaag
     1921 acctacctac ttttgaggtt tatgatgcac ctcaacttgc aggtatcagg tctaaaccca
     1981 aaagagcttt aacagtagtt ataagtactg tagccgcttt tattctatct tgccttcttg
     2041 ctttgataat tgaaagcttg cagggagaaa ataaagaaaa aacacaggca attaaagcgg
     2101 ctcttttcgg aagaaagaag gcctcctgaa ttccgcaact ttaagcgttc cttacaataa
     2161 tatcagatta ggcaacctga taactccctt tctgctaata agtttcgttc tggaatgctt
     2221 tccggtagca atgggaatta acaatatgta ttttttgttt gttgcctgcc tgtgtataat
     2281 ctacgcctta ataaatccgg atttaatcgt tatcaacaag ataaatttcc ttcctgcaat
     2341 gtttatgctg gcttggacaa gttattcctt tttaagttat aattggtctt tcgtgaaaca
     2401 ggtctcggtt gttcattcca tactgatatt gaaaaaagca atgcttttta ttatagtaag
     2461 catcttttgc cgtcatcaga cggtgaaaaa atatgctcct tggttctggt tttccgtgat
     2521 tttggtttat attgtaattg ctctatggga aatgcttacc tggcaacatc tgcctcaatc
     2581 gctttattat aataagacca attttgttcc tactggtcct ttttacggac ctaatcagtt
     2641 agctgccgct tttaatctgt tacttccttt tatcttgttt cttccggaaa taaaaaagag
     2701 caagttttgg ggggtaattt ccctgctatg ttttttcact ataatgggca ttatagtcat
     2761 tgaaggtgcc agaattgcta ttattgcctg ttcctgtttg tttctgtttg cctttatttt
     2821 tctttattct gcacgaatga gaaaattaag tttattcctg atggctttaa taattgtcgg
     2881 tttggtaatt tttgccaaac ccctcgtttc ctttggcaca gaaatgttta ccgaagaaat
     2941 aagcagtatt ggacaggaat caaagtcctt cacaatgagc agcattaaaa tacgcacccg
     3001 cttaattccc caaaatttgg aaatattggc tgaaagcggt tttaagggtg ttggagccgg
     3061 aaatgtggaa cattatatgc tcgcaggaag aatgtataaa acaggcggaa tttccaattc
     3121 ccataatttc tttctggaac tgcttaccaa ttacggcata gttttctttc tgggttttgc
     3181 ttatctttat gtgctgtggc tttacagatt gtggcgtgct ataaaaatga acccggaaaa
     3241 cagacattat tacgaaatgt atttctttgc gcttttgatg ttcctgccaa cttctattct
     3301 acccagttct attatttggg aacatcatta ctggatactt tttgctatta ttaacgaaat
     3361 ttcgcatccc gataactgga tgccgaggca atatgaacaa atttgaacaa ctggtagaaa
     3421 tagtagctac cttaagaact cccgccaaag gttgcccctg ggatttgaaa cagacccgcg
     3481 agtccttagt tcccaatttc attgaagaac tgtatgaagt agtggaagca attgaaaata
     3541 aggattatga tgctttaaaa gaagaattag gcgacctgat gctgcacatt gttatgcagg
     3601 cacagatttc taaagaacag ggtttatgga atatagacga tgtcctggaa gaaattagca
     3661 gtaaacttat ccgccgtcat cctcatattt ttggtgacct ccatttaacc gatgctgatg
     3721 aagttaaaca aaattgggag cgtttaaaaa aagcggaaaa aacagaacgg aaaagtgttt
     3781 tggacggaat tccgaaatca ctgcctgcgc ttattcaggc acaaagaact caggaaaaag
     3841 ctgcttccgt tggttttgac tggcaggata taaaacccat tctggaaaaa ctggatgaag
     3901 aaagggaaga acttgctgaa gctctgaata gtaatgaaca agatgccatt aaagaagaac
     3961 tgggagatat gatttttact ttggtaaacc tggcaagaaa gttacatata gatgcagaat
     4021 ccgctttgaa ggaatgcatc cgtaaattta cccgcaggtt taatgctatt gaagaacatt
     4081 accttaaaaa cggagaagac attaatgaag ccggtcttga agaactcgat tctcactggg
     4141 aaagaaccaa aaagcactaa tctcttcaat cgtcttcggt ttatcctgat tttcggcagc
     4201 ttggcaatct ttattttctt tgctgtctat attcagtttc tcctgaaaca ggcaaaaaag
     4261 gaacaggaat ttattccgcg cattttcgca cagtatattg cttacaccga tagatattta
     4321 agagaagcgg aacgcaatgc ccaattaatt accgaaataa gttccaaata tttgcaattt
     4381 actacttcca gggactttaa acaagacctc tgggattata tcagcactga atttatgcag
     4441 gaaaatccgg ttcccgtaat cattaccgat caaaattttc aacccctgct gtggaaaaat
     4501 gttggtgtgc ctacagatac catttttcac gagctttccc ctgaaaatca gaaaaaattg
     4561 gaaaacttga tgaagacgat ggttcaaatg cctttaacgg atgatggcaa aattagcggt
     4621 tatgcctatt acagtaaacc cgtttctttt gccgagttca ttaaaaatgt agattgctca
     4681 attatcgtaa ccgaccacta taaacaaccc ctcttctgga gaaatgtcgg tattccggaa
     4741 acagccaatt actatgaaat ttctacggaa gaacagcaaa agctggctca tcagattaaa
     4801 acgatggatg aaattccgct ttctaatgct gcagatagtt tgggctatat ttatttttcc
     4861 actcccaaat ccctttccta tatacgctac atcgttattt tggaattgat tttggctttg
     4921 atggttgttt tctttggcat ttatggcttg tttttactaa gcagaaccga aaaagatacc
     4981 ctctgggtta gtttagccaa agaaactgct caccagtttg gaacccccat aacttcttta
     5041 atgggatggc tggattacct gaaagaacct tctccggatg gctgtgtgcg cgatatagat
     5101 caaattgtgc aatatatgac tgccgacctg gaacatttga aaacaatcgc ttcccgcttt
     5161 ggaaaagtag gcagtatcac taaactggaa ccgcaaaatc tggaccggat tatttctgaa
     5221 acagtagaat actttcggga aagaatgcct cacctgggac acaagatcaa tattcattac
     5281 atcagcaaaa ttgaaggagt tatggtactt atggataaag acctcattaa atggtcattg
     5341 gaaaatctga ttaaaaattg cgtggacgct atgagccaaa aaggcggaaa tatcattatt
     5401 accgctaccc ataaaaatcc ctggatttat attcatatcc gtgatgaagg aaaaggtatt
     5461 cctcatcgcc aatggaaacg catttttgaa cctggagtta ctactaaaac ccggggttgg
     5521 ggtttgggtt taagcttggc taaacgtatt attgaagaat accataacgg acatataaaa
     5581 gttctgcaaa gcacaatcgg cgaaggcact acttttgaaa taaaacttcc tgttcaggaa
     5641 acaaataagg agaaataatg gcttatgttt ttaccccctc acaaatgaaa accctggatg
     5701 aaaataccat caattgtttc ggcatcccgg cttctattct aatggaaaat gccggaaggg
     5761 gctgtgccga ttttctgatt aataactatc cggaagaatc aaaaggggtt attattatcc
     5821 tgcacggtta cggaaataac agcggagatg gctttgtcct tgctcgttgg ctccaaaatt
     5881 acggaaaaca agtcattctc ttacaagttc agaaaggaga aatgacagaa gaaactcgcc
     5941 acaattttaa actttgtaaa gccctaaaaa tgcctacttt tgaattaaac aaggaatatg
     6001 ataacctgga tatcttagaa aatgtaccag gtgttacaat gattattgat gctgtcttcg
     6061 gaacgggatt taagggtaaa ttgcctgcat ctctgaaaaa aatatttcaa agcgttgatg
     6121 atacctctgc tctcaaagta gctatagata taccttccgg actaaatgga gctacaggag
     6181 aaggagaaga tgcctttcgg gcagatgtaa ccttaaccct tcattcttat aaaatggggt
     6241 tctttttacg agaaggaaaa attaaatgcg gaaaggtcta tactatcccc atcggaattc
     6301 ccgagcatta taacgatgcc ttttttactc cggctatttt tattgataat aaaacatttc
     6361 gcttgcccga acgctattat tatgctaaca aaagcaacta cggcagggtc tttataatcg
     6421 gaggaagtta cggtttatta ggtgccgcag ctttggctac aagagctgcc ctacgtgcag
     6481 gagccggttt ggtctattta atcagtcgtg aggaaaataa gtatttctat aatgccaatc
     6541 ctgcggaatt tttatttatc ggcatcccca gaattgaagg aacattaact cccgatctca
     6601 aattatttac tacccttttt aaaacagccg atagcgttgt tataggtccc ggcttgggat
     6661 ctgataatta tggcacctat atgctggaat atattttaag aacatctaca gttcctacag
     6721 ttgtagatgc cgatgcgtta cgcataattg ccaaagaccc ttctctgcaa caatatctta
     6781 aaaaacctaa tatcctgctg acacctcatt tgggagaatt ctgcacccta acagatatgg
     6841 atatggataa actggttaaa gatattccta ccgccttgga aaattatatt aaaaagaccg
     6901 gggcaaaagt cctcctgaaa agtgatacta caatctttag cgatgggaaa agaatgttta
     6961 ttaatatcag tggtaatgat ggacttgcta caggcggcag cggagatgtt ttagccggta
     7021 ttatcggttc cttttgcgga caaaaaatgg aactgggaga agcagcaatc aatgcatctt
     7081 ttattatggg taaaacagct gaagaacttg ccaaacaacg ctatttccct tctatccttc
     7141 ctacggatat catagaaaac ctgtttatgc ccctaaaaaa tgaaagttta tcttctccgg
     7201 aaggttgatg ttgagtaatg attaagaaag aagaaagaaa aatgattgaa ttgaactgag
     7261 aaagatggac tatcaataag cccaaaatac ggtttttgct attcctatac attccattcc
     7321 tgtccctatt aagtccgtgt tatccttgta atctgtgggc tatttttttc agtatcctca
     7381 gtgttttcgt tgtttttagt gtaaacataa tctgcgtaat ctgcgtgagc caaaaaacca
     7441 gttttaccat tttgcttgac acaaacagca ctttcatagg ttggtaacta aattgcagat
     7501 aacaaaaagg atactaattg atgaaggaaa aaatcactcc caagcgtgac aaaacaaaat
     7561 ctgacaacct gccttacact ttaactcctc ctccggaggg aaaaaagttt cataaacgca
     7621 aacccggttt gcaggattgg atggaagcaa ttctttttgc ttttgtggta gcaatgatca
     7681 tccgcaatta cacttttgaa aattttctga tcccttcttc ctcaatggaa aaaacgcttt
     7741 tagtgggtga ttacttgatt gccaacaaaa taaagtattt ttttaacgat cccaagcagg
     7801 gtgaaatagt cacttttcgc tatcctaaaa tagaagaagg cactcctgaa cacaaagatt
     7861 ataaagatga ttttataaaa atctttcccc ctatctatat caataaaagg catagttttg
     7921 ccaagcatcc tttcactgcc tttcatattt gttattatgc ccgcagaaat gtagtgaaaa
     7981 gagttatcgg tatgcctgga gacatagttg aaatcaaaga caaaattgtt tatgtaaacg
     8041 ggaaagaata caccggcggt tttgaaaatt acggtgttgc cattcccagt ccacctgccg
     8101 ctccggaacg aattgaatat tttacctatc cggaaaacag ctttgattat gtttcaatga
     8161 acccttggta tgaaccctac agagtaaaaa ttgaaggtga ttcttcagct gtgcggaaaa
     8221 tatttaaccg tgactggttt ggtcctgtca aagttccgga aaagagttac tttgtgatgg
     8281 gtgataatcg tgatgttagt gaagatagcc gttattgggg tttcctggaa cgaaaataca
     8341 ttactggaac tccctggctg attttcttca gtagaggtat tgaatttaac aaactctatg
     8401 acgaacctca tatccgatgg aacaggattt tccgtcatcc ccgttagggc tgtatatcca
     8461 tattcctttt tgtcttagcc gttgcggata ttgcagtttt ttcagtgtac cttatcgtaa
     8521 cagtgtttta aatgaatatg ttgactatct ggaaaaggaa aaatcccttt actccaatca
     8581 attaaaccgt cctttagcca caatttactt tggcggtgga actccttctt tgctaaatgc
     8641 cgagcaaatt aggcatattt taagcggact gaatattctt cccgaggcgg aaattacttt
     8701 ggaaatcaat cctttacaaa taacccagac ctatctgcaa gcacttaaaa caacacctat
     8761 caatcggtta tctatcggac tccaaagtat gaatcaggaa gaactgaatt ttctggaaag
     8821 acatcataaa ccggaacaaa ttcaggataa aatagctctc tgccgtcagt ttggctatga
     8881 aaatctttcc ttagacctga tttacggttt gcccaattct tctcttcaga ccttgcaagg
     8941 gacagtggaa aaatacttgg aactgaaacc ggaacatatt tcctgctacc tcttaacttt
     9001 ggatgcggat tgtaaactgg caaaagagaa aaataatttg cccaatgaag aaattcaggc
     9061 agagcaatat gctttccttt gtaactattt ggaaaaagag ggctatatac actatgaaat
     9121 ttccaatttt gcccttccgg gttatacctc tcaacacaac ttaagatatt ggcataatga
     9181 tgattactta gctttaggtg cctcttctgc aggttggatt tctcctattc gttatcaaaa
     9241 tcctgcagac ctgaatctct attatcaaag catagaaaaa ggtgaaacat tcccacagaa
     9301 agaagaactt tcccagcaaa gaataattca ggactatctt atgatgggtt tgcgtttaaa
     9361 agaaggtatt gatctggatg aattttacaa ccgtttccaa gttaagctta ccgaactata
     9421 tggagaaagg atatttaaac tgcaacagat aggaatgctg aatttaacag agcataattt
     9481 ggcattaacc gaaaaagcat tatttgtttc caacagcgtt atcggggatt taattctatg
     9541 aacttgaagg aattgtgttt atttctcaga gagctaattc aggacagtga atttgctgat
     9601 aaaagttatt ttgccggtgg ctgcgttagg gactttatct taaaccctga tgcaacccta
     9661 aaaggtgatg tagatatctg tgtggaactt cctgaaggag gaattgccct tgcccaatat
     9721 ttgcagaacc gattgcaggc aagtgaatta gtcattcata aaagtttcgg aactgcctcc
     9781 ttactctata aaaacttgca actggaattt gtagctaccc gcaaagaaca atatttcccc
     9841 aataaccgct atccgaaagt ggaatttgga actttagtag atgatgttct cagaagggat
     9901 tttactatca atgctttgct gatggctatt gccacaggtg aaattttgga tttatgccaa
     9961 aaaggactga acgatttgca acagggaatt atccgcacag taaaagaccc gaacttaagt
    10021 tttatggaag accctttaag aatgcttaga gccctgcaat ttgctatgcg ctttaatttt
    10081 actattgaag aaaatacctt taaggcaatg cagaaaaatg ctcctgcctt aaaaattctt
    10141 tcccatagag ctattcaaaa cgaactgcaa aaaattccgg aaagccaaaa aagcaaagct
    10201 aaacagctga ttagtaaact tggttgggaa ataatattgt ctctattttg aatttaccgt
    10261 aatattatta aaactgatct ggatttctgc ttttacagga atgctaaatg gcttttcatt
    10321 taagaaatat aaaaagccat tcttctcagt attattagtg ttttcggtgt tctcggtgta
    10381 aataaacccg ggctactttt ttatcagaga cagcacttct ttcagtgtat ccctttctgc
    10441 tatccaaaga tatattccgt aacctataag caccaccagc ggaaaaacaa tagagaattt
    10501 ccccatatac agacatccca acgcaagcac aagcaacaca ggaagataag ttaaaatatc
    10561 aatagttatc tgccataaat ctgcttttaa taaagctaag gacatatagt aaaaagcaaa
    10621 gtgcataact gcactaacta aacaaaacag caggatagca aaggcaaaac ttacctgcaa
    10681 acccaaatac agcactaaaa tacgcaaacc aagagtaatt atattccaca gcaattccca
    10741 ttgcggtttg cggcaaataa atggtattcc actaattgga ttatgcagca tactggttcc
    10801 ataatagatt accaaataaa gaattaacgg cattgccgcc agccatttat caccccaaat
    10861 tacaggaaca atatactgta aaagaagagc ataaacaatc aaagcaggaa tgcctatttt
    10921 taaaaccaga gaagtataat gtcttattcc tgcaacactt gcggactttt cactctgagc
    10981 taaaactgga aagaaaacct gagcaaaaga agcattcaac atccctacgg gaatagcaat
    11041 caattgtgaa gcaaagaaat aaacacccat taaagccggt tccaccaaag tccccaaaaa
    11101 aagaatcgga gcattgatag aatatgcctg caaaagattt atggcattaa ccataagcag
    11161 gaaattacta ttacgggaaa aaagtgcaag cgtctttttc agccacttaa agctaaaaac
    11221 ccttttcagt aaagatagag gcaagcgcgg cagtttaaca cacagataaa ctaattcagc
    11281 cagattgcct atataaaata gcaaaataaa caaccaagca taacgccaaa aatagataag
    11341 tgcaaggcaa agaacagaat agattataac attgattgtc tctgccaaag ccaggtcttt
    11401 aaactgaaac ttcttttgcg cataaacacg cacaatccta cgcagagatt caataaaaat
    11461 caaacccccg ccataagaaa gaactttgcc cagttctgca ctatgataat aggcaccgat
    11521 gtttttacca aaaacagcca ataaaacagt tataatagca gaaacaagaa gtcccgtctg
    11581 caaaagagcc aggaaattta ccctttcttt ttgtctggaa acaagataca cataatccag
    11641 ccccaaatga gtaacataag taagcaatgc caaaatagcg gaaaaactgc ggtaaatgcc
    11701 aaaatcagtt tgcgttagcc attttgccag aaagataaaa aggattaaac tgatagccct
    11761 tctcaaaata gttgtagaaa gagtccagcg aatgcctgaa atcactgttt ttgtgtgcat
    11821 ttgcctctgt taaaaaatct ctgtctgcgc ggaggcagga tttaaaccta agtgcttgta
    11881 tgctttgaaa gtaacttttc taccctgggg actgcgttcc agaaaaccct gctgcaccaa
    11941 atagggctca aaaatttctt ctatggttcc ggaatcttct ccaatggcag tagcgattgt
    12001 tttaatgcct acaggacctc cattataatt ttcaataatc gtcgttaaaa tgcgtttatc
    12061 catttcatcc aagccggcat tatccacctg cagcatttgt aaagcagaca aagcaatatc
    12121 taaagttata attccttccc cttttatttg agcataatca cgcactcttc taagtaaacg
    12181 attagcaata cgaggagtgc ctctactacg tctggcaatt tcttgaacgc catcatcttc
    12241 cgttggaata tttaataagc gtgcacttcg gcgaataatt tgggcaatgg actcctgatc
    12301 gtaataatcc aaacgcagaa taataccaaa tctatcacgc aaagggggag taagcaaacc
    12361 cgctctggta gtagctccaa caagagtaaa atgttccaga ggaattttta aggtgcggga
    12421 attgggaccg ctatccagaa tgatttccat ttcaaaatcc tccatagcgg gatagatata
    12481 ttcttcaata atatgggaga gacgatgaat ttcatctata aacagtgttt cgtgacgctg
    12541 gagattagtt aaaatgcctg ctaaatccga gggtttttca ataaccggac cactggaaac
    12601 agttatatta actcccattt cgcgagcaat aattcctgcc agggttgttt ttcccaaacc
    12661 gggaggtccg tataaaagga tatgatcaag cgattcccca cgcagtttgg ctgccttaat
    12721 actgatatcc aaaagttcct tgatatgagt ttgcccgata aattccttta aggttctggg
    12781 acgtagggct ctatccagct ctacatcttc attcaattta tcgggattat tgattctttc
    12841 cagcattagt tacctgtttt tttcgctatt tcagcaatcc caaaattctc ataaattcca
    12901 gtcgccaaac catccaggca gcttcccaaa cgattcttcg gctcatttta gagacgccgt
    12961 ttttgcgttc cgtaaagaca atagggattt ctttgatgtg aaaacctttc acccaagctc
    13021 taaaattcat ttctatctga aaggcatatc catcagagag gattttatcc aaatcaatat
    13081 tttccagaac cttacgctga aaacatttga atccgctggt tgggtcttta ataggcatcc
    13141 tggtaattgt gcggacatat ttactggcaa agtaactgat tagcaatctt ttgataggcc
    13201 agtgaataat atttacacct tcacaatagc gactaccaat tactaaatcg tattttttgg
    13261 ctgcatttag caagagagga atatcttcag gattatgaga aaaatcggca tccatctcta
    13321 taatgtaatc atagtccctt tccaaagcat acttaaaacc tgtaacataa gcagaaccaa
    13381 gacccatttt tcctggacgc tccagtatat gaatgcgggg ttcggaactt tgcataaatt
    13441 taactcttaa agcagttcca tctggagagt tatcatcaat caccagcact tcaattgttt
    13501 cgcttttggc taaaacctgt tccaagagct tttcaatatt gtctatttcg ttataagttg
    13561 gtataattat tagtgttttc attgtttatc cagattattt tccagcaatt ggggttcggg
    13621 aacttcgcta aatggctttg ccggatttcc tgccacgatt gttttagcgg gaacatcttt
    13681 ggtaacaact gctccacctg caacagttcc atcactatga attgtctttc ctggcaggat
    13741 tgtagcgtta actcctatcc tggaaccagt catcattgtt actcctttga aatgtttaaa
    13801 gcgttcttta tcgcgaccca tataattatc gttacttgtt gctacacaag gcgctataaa
    13861 acaatagtca ccaatttccg aataggcagt aacatagcag tttgtttcca gtttatttct
    13921 gttgcctata tgcacaaagt tctcaatagt aacatttctg ccgactatgt ttaaatcgcc
    13981 taaagtaaca ttttcccgta tagttgccag atcagcaatc aaattacggt taccgattgt
    14041 gcattgacaa taaataatta cattactgcc aatctggcaa aaatcaccta tttctgtgcc
    14101 tttcaggtct gttggcactg taaaaatact gcgtggagag gaaagcggtt ttttgccaat
    14161 tatggttcca tcatctatcc ggcaggcatt tccgattttt gtatccggat gaataataac
    14221 attatgccct atcagacagt catttccaat ctggactcct gccataataa cgacattatt
    14281 gcccaaggtt acattctttc caattttggc actttcgtca ataaaccggt tcattcgtcc
    14341 tccctttttt tacttaatac cattttttgc ttgataagtt ttaaggcaag aaaaaaaatg
    14401 gatttatgga aaaatatata gctgatttct gcaattatct tgctttggaa ggtaaatcgc
    14461 ccagaacgat tactgcttat aagctggatt tggaacaatt ccattcattt attcagcgct
    14521 attttgaaaa tggcgaagtg gatataaaag gaattaccgt tcttaatatt cgggattttt
    14581 tccgctttct taatgaaaaa ccggattgta accgctcttt agcgcgtaaa agtgctgctt
    14641 taaattcctt tttccgctat tgtaaaagaa gcggttttat tcagaataac cctatggaaa
    14701 aaattaagcg tcccaaatat gaagttcctc ttcccaaatg ttttaccgaa gaagaagtgc
    14761 gcactttgct ttctattccg gataccgata gtcccttcgg aataaggaat aaggcaattt
    14821 tggagacctt atattcttcc ggattacgta tttccgaact tgcaggaatc cgtttgcagg
    14881 atattgattt gaaaagagga ttgataaggg ttacaggaaa aggcaataaa cagcgtattg
    14941 tgccccttgg ttcttatgct attgaagcaa taaataacta tttaaaggtg cgtccccaat
    15001 ttatgcgaga aaataacccc gatttgctct ttttaactaa aagcggtaaa gcatttgaca
    15061 caaaacagct ggatattatt ctaaaacgct attttgaact cgttgccaaa gctaagggct
    15121 attctcctca ttctttaaga cacagttttg ccacccatct gctttccaga ggagcagatt
    15181 taagagccat ccaggaactt ttaggacact ctctgctctc tactacagag acctataccc
    15241 acatttcgct ggaagatatt aaggaggcat ataaaaaagg gcatcccaga agcaaggaat
    15301 gaacccgatt aaaaaatcca taattctatc ccttatgctt cccatatcgt tcccatatcg
    15361 ttcccatatc gcaatatggg aatcaattgg gatggttaaa tgcgatatag ggctgaaaac
    15421 aaagagaaaa agcagttttt tcttgaagat ttaagttaga aattatagca cgcaactgac
    15481 tggagccatt ggagttacca gattaaattc aggcgtaatt agcagtataa taaatatctt
    15541 attatcatct tctcggtgtt cacggtgtcc tttgtgtaaa aaagagaaaa atatatgaaa
    15601 aagattcttc tgacagccat aaacagcagt tggagtcaat ccaatcctgc aatatattac
    15661 ttacgcgaaa tgattgcaga tttgccttat gctgttattc tccatgaatg gacattaaaa
    15721 gaccggctct ggaatgtgct gtctgaaatt tattgccaaa aggcagaagt aatttgtttt
    15781 tctgcctata tttggaaccg tctttatctg caggaattga ttcctgaagt tatcaagtta
    15841 ttgcctgaag cagttattgt aattggcggt cccgaagcgg aaagtttatc ctgcctgaaa
    15901 agaaaaggac tttatattgt ccaaggtgcc ggtgaaggaa aatttcgctt tctggcagaa
    15961 cacgattttt ctgctaatga tgaagacctt tctcgggcta aacatattcc cttgaaagat
    16021 attcctttcc cttacagaga aagcgatggg gagattttaa aaggcaaatt gctatattat
    16081 gaaacattta ggggctgccc ttttgcctgc atttattgtc tttcggcaaa tgataaacgc
    16141 tccgaacttc gctatcagcc aaataaccaa aaagatatgc aatgcctgat taaggaattg
    16201 gagaccttga tttcttttca gccaagaact ataaaattcg tagaccgcag ttttaatctc
    16261 cacaaacagc tggcacacta tatttggaag ttctttatga aacaaaattg cccttgtgat
    16321 gtccattttg aaatctaccc tgatttgctg gaagaagacg atattgcttt actggaaaaa
    16381 gcaccggaaa atagaatccg tttggaaatt ggtattcaga ccataaactc cgaagtagct
    16441 ttgaattgcg gcagaaaatc taattgggaa aagacaaaat ggactttgca agaactgaaa
    16501 aaacgaacta atatacgcat ccatactgac ttgcttgccg gtttgcccgg ggaaaattat
    16561 gcttccgtat taaatggctt aaatgaggtc tgcgctactc ttccggatgc tgttcaactt
    16621 ggcattttaa aaattttacc cgataccccg atgcagaaaa ttgcctcgga attgaattat
    16681 aaatggctca gccaaccacc ctatcaatgc ctttcttccg atgctctttc ttttgaggaa
    16741 atacaacaac tggagaattt tgccaagctc ttaaacctgt attggaataa agaagaacac
    16801 aaaagtatgt ggcaggaaat gctgcaaaca caatctgcta cggatatttt aacggctctt
    16861 caacaaaaac accaagaact tggctatgaa ttgcattctc tatccaaagc taaaagaaat
    16921 gctgttatag ctgatatctg tgtagctgga ggacgccgtc cctccgcaaa aaagtagaaa
    16981 aatgaggaac taaaaaaatc gtaaaataat gcagagcttc agcatgctcc agctatgcgg
    17041 ttaataatat atacttgttc taatcgtcat aagccctttg caccttgaaa gatataaccc
    17101 aagaaaaaat attagggcgc aaacaactcc tgatttacat attagttgac gaggaaatca
    17161 accttccgga aaagcttgtt gcctttttac cttgctcaaa aagcgcagtg atatgctttt
    17221 ctgcttgagc aggaataaac ttgatagttt tgcttaattc agcaatttta acagctgatg
    17281 gctcaaactt ggcaggaaca taattctgaa gggttaacaa caatttttct gcttcctggt
    17341 tccaaaaagg cacttccgca ctattgggat gaatccaatt cagggtataa aacaaacgca
    17401 tcaattcatt gataataatg gacttggcag gattggcgct ttttgttatc tgccccaaat
    17461 cacttaacgc ggttccttcc atttccagaa aacttggagc cggaattaac aaatccacag
    17521 gtgctttttc atcctcaaaa ggcattatgg caacactaaa ttttgcttga ttggctaaag
    17581 cgggatagac accccaactt aaaacaaaat ccgaactgga aggagagaaa tccggctgca
    17641 aagctaaaaa tccaagatga ttacgaaaat cggtagaaac cagaagatta tcagttcctt
    17701 gttcagatgc aaaacaccat aaggcaaaag cttcctgttc cgaaatgcga ttttgattat
    17761 agataaagag tgtcttatcc gttgcctgaa cttctgccaa cttggcataa acagcatcgg
    17821 caaaacgaca aaacgcattt tccggatagt ttactaaagt taatttcttt ccctttcttt
    17881 gctgtaagcg gattaaagtt gccagcacct gatttagttc tccataaaca acatattctt
    17941 caaagtcatc cagaatttta tagggttctt tttgcggact taagtttaga aaaacatcgc
    18001 tgaaggcgga aaaaacagga ttggcagaaa gttctgtctc atatttatga gcagctcttt
    18061 gcagcatcag catttcttcc agagaaatat aaggactaat ttcaaagcgt tttgaactgc
    18121 ataaggaaat ttctttggct attaaatcaa ttgcttcctg ccagccgatt tctttaaaaa
    18181 tgccgttttc cctaaacagc ggaactttca atctatcttt atcataaaag ctctgccagc
    18241 caaatcttcc cttaaaacag agatttttac cgttaaaagc aggttcctct tggggagttg
    18301 taacccgggt aacaattcct gcttgtgttt cggtttgaat aaaacagccc actccacata
    18361 aaccacaatt ttgcagagag atatctttgg gatgcggatt gaatttatag tgaacagtcc
    18421 tttgcattaa agcacccaca ggacaaacat caatacattt tccacaagat aaacagcttg
    18481 ttttggttaa cgaatcccca aattcgggag ctacaagtgt ggcaaaacct ctataaatat
    18541 agcccaaaac tgcaggacct tgaatttcgg cacaggttct gatacagcgt ccgcaattaa
    18601 tgcacttatt ggcatcccgc ataataaaag gatgactgta atcaattgga tgtttatttt
    18661 tatcccccaa aaattgagca ggattaacta cataatctgt gcaatattta cgcaggaaac
    18721 aggtttcatt tacaaaacaa ccacattcca aacagcgtct tgcttcactt acggcatcat
    18781 cttccgaaaa tccaagctcc acttctttaa aactgttttt tgcctcaatt aaaggcagtt
    18841 ccggcatctc tttgcgctca attttttcat atatagaata ctcttctggg ctaacttctt
    18901 gtaatgtatt agctttttta ctgtcaaaaa gcatacgctc tgcggctatt tctcccgttt
    18961 ccaaaaacct gtttatagcg gttgcagcta ttctaccatc agctatagct tcaattgcag
    19021 tagcagcccc tcttctaaaa tcacctccgg caaaaacatt tcccaagccg gtaaacatag
    19081 ttgattcatc tacaatagcg gtctgccacc gcgaaacagg caatatttta tctgctatat
    19141 aattattctc ttgagtgaag atatccacct caggaacctg agaaatagca gcaataatac
    19201 tgtcaaaagg ca
//
LOCUS       DAOPFL010000028        18951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_3175_length_18951_cov_103.634367, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000028 DAOPFL010000000
VERSION     DAOPFL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 18951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18951
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3175_length_18951_cov_103.634367"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="PLG69_03495"
     CDS             <1..1716
                     /locus_tag="PLG69_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42605.1"
                     /translation="PEQEDLTEWEQPTPNDTRFLFSFYGAQPENPSEYSDPAKRWNLP
                     PKKTMKIVVAVFPGDNKEDLKKSAGWAKEIYGQAQDLITVTQPDTFPHYTPPEPPDIP
                     NLYAQLQDDGNRLDVYWDNRSEFSYDVKTVSTAIIGWQNPESSLLISGLDSDPTPYIA
                     NNWVDIPEEYRPGPPPEPGMEDKRWNMNALVNPYTASRLRKDFQGYTLWGRSGSGSQE
                     DWEMIKRWDKVDTPRDHSDYTVNSAFPDLFRDFGGYLGIDTGLPNKNEWNVDVSEYHK
                     FYHFDENYQLVPNGDTFYGWPIYTPDIVINGTTYNEVTDWQSLQNYANSIIGPDLETT
                     QLLQARIFKHQDIPDHIFYALYDPKLIPLQGFAFPGGEGAEAPDTLDLSNLRKERLAR
                     RYYKSYIMYPRKGVEYYVALTAFDRGIPANDLNYLESGRDADANMKVFFPGTLAKENM
                     DNIMVIPNPYIGRSSFDGRRENDEKGDKSRRIWFINLPKRCDIRIYTLAGDLVQTLKH
                     DGAPVTDIITISKASSTGLAADGMEAWDLLSKNKQIIAPGVYLYSVENKADGKNKVGK
                     FVIIK"
     gene            1727..2758
                     /locus_tag="PLG69_03500"
     CDS             1727..2758
                     /locus_tag="PLG69_03500"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPI42606.1"
                     /translation="MKKVLTLVLIITIAGLPLIITAKPFGKTGTVALQFLKLGVDARA
                     VGMAEAYTAVTDDISSVYWNPAGLAPAFENQVFVSHTNWPASIMHEFGAATYTNGVST
                     IALYGSILHMDDMDITEEEPFGPTGEKFTCSDIAIGAAFAQQFTNKFSAGLGIKYLRE
                     NLYEFNVNSYAVDVGSMYNTGWRNIKIGMAMRNFGPDIRFRVDDDEDGKDDEDPFDLF
                     DNDGDGLVDEDGIELDSKIPLSFSLGVSGDLMRKGNNYWIASLQLDNVIDRLETWNLG
                     TEYKLGNIFFRCGYQFNYDTNGFAAGVGWQVPTSFGIFNIDYAYTDMGDLAESFTKGA
                     HRVSLKIKY"
     gene            2788..4971
                     /locus_tag="PLG69_03505"
     CDS             2788..4971
                     /locus_tag="PLG69_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI42607.1"
                     /translation="MKKILLVILTLSLLVGIVFAQEMIPTPDNKKAMETPVPKKIIPS
                     ERTAPVYTFTKTPHSLLTSYYDYMIGSYNGLPLRVIPSNILGGGYFLTYHGRRTSTGT
                     RRIFYAYLDANGDVIVNNEITTEANNEGYGTVAVDPVSGKPFYAWHANSDTDAELEVQ
                     FTVDQFYEGIYGLFTPLVDAIDNPSTVYTSDGNFTDDNEFIWPTAVIGPSPYPDKRRI
                     YVIARNSITHTYGPSENPKIAYADFDAFQIEQNVPLTWQYTSIPEMDEWNVDSEWRRP
                     FNSLTADNAGNLYYAGFHFATEADGSTDIVEPDVDVFQCGNYGEGTWSRTSSWGNLPT
                     WNPNTSPTDTTGFFKNQSELPYGDNDIYWELFSSASGHLNATVDDLGRIHFPAVWRLV
                     TNDGYWFPNFQLPKEIIFDPSLPEDSQFRINDIWPRKDPTNDVDEHYQPWDSQPPWGV
                     EDFVQDPDTGEWFVDMYLSWEFPYWDQNAHDSAMFFHCNNMKVTENNGHGMLAMVWQN
                     SARAKWYNADGDSDYSAWANTPEIWISVSGDNGNHWSEPIKLNNIETPEFAGIKPMWV
                     YPADKVIYVGESNGEKVGKLGLLFYDDFTWGAFVISPGVGPMNDGGRVMFTELQIVFP
                     SDASDDPTVSPVTNMLNQNYPNPFNPETTISFDMPKTANANLSVYNVKGQLVKTLHNG
                     IANFGRNTVVWNGTDNNGNKVTSGLYFYRLTTDGKVETRKMMLMK"
     gene            complement(5211..6134)
                     /locus_tag="PLG69_03510"
     CDS             complement(5211..6134)
                     /locus_tag="PLG69_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPI42608.1"
                     /translation="MKKILILILWFLIGVGIIIAYLTLGKPDTNIITQVRIPRLLLTL
                     FTGTALAGIGSIYQLMLANPLAEPYILGISSGSAFGSILLGISGLIILMPVGGFIGAG
                     FTMLLVWKLAHQKGRFDKSRLLIAGVIAGMFFSAGISLIMYLHQQDTALILGTLMGNL
                     GHIFTKGEWTSFLILMGLSLIILIYLFFQSTAIDILSCGDLYAASLGINVLSLRRKIF
                     ILTSIVIGISVSYAGIIGFVGLIIPHIVRFFIPSGQKKIYLLSLWSGGIFLLACDLLA
                     KHILSMELPVGIITAFIGCPFFMWLLLKKQG"
     gene            complement(6131..6976)
                     /locus_tag="PLG69_03515"
     CDS             complement(6131..6976)
                     /locus_tag="PLG69_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPI42609.1"
                     /translation="MKKSGILTVIIIILFAFSCKQTGNNQKKGIVVLSPEVAEIICAL
                     NAEKEITGITAECNYPPSLQQKKIVGSFSSIDKEAIIALNPELIFCSSLEQENIAYDL
                     KNLGFQVEVIYPKGINEMLSDIERIGTLIRKKEEAVKLVNYLKEEIEKIKANAQGKLR
                     PKVYLEIYRNPLMSVSDSSFIGELIELAGGDNIFSTLERDYARINPEAVIAAKPDIMI
                     CYSQDTLKNIINRKGWQNIPAIQKRRIYFEKDINPDLLQRATPRCIEGMKELAKIFAE
                     WREEK"
     gene            7616..9139
                     /gene="cls"
                     /locus_tag="PLG69_03520"
     CDS             7616..9139
                     /gene="cls"
                     /locus_tag="PLG69_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPI42610.1"
                     /translation="MGLLISLVLIASLTQIIVGGSVLDTFSKVTLRIINILFGISIVL
                     IILIVVMENGSPATTMAWILVLIFLPVVGFILYLFFGRNWRKKRLFSKKGYADTKLLL
                     SYVHNHPSDRENNWQNDLTARLYNLLKNNSKATLTVNNNLTLYNDTGKAFKAILEGIN
                     SAQHFVHLEYFSVFADETGYTLQKLLIQKALEGVEVRFIYDDVGCWMLKKSFKQELRD
                     AGVEFVPFMPVWIPFLNSRLNYRNHRKLVIVDGKKAYLGGMNIGDKYLGKKRYYGYWR
                     DSLAEIEGKAVNALQAIFLADWNFVTGKNLMQEDLLHKYLPVISPDKQRFLPMQIVAD
                     GPDSDYDSIMQLYFAAITYAKHSIRISTPYLILNESLLSALKTAAISSVKVQILVPDK
                     PDHFLVYWASRSYFQQLLDVGVEIWTYHKGFNHSKILIVDDEFLLIGTANMDLRSFKQ
                     NFELAAAIYDKTVCAEAIKQFDEDLLSSGKIIPEKFRQRSMLQKTKESICRLVSPLL"
     gene            9262..12084
                     /locus_tag="PLG69_03525"
     CDS             9262..12084
                     /locus_tag="PLG69_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPI42611.1"
                     /translation="MKFINYPLSENLDTIVQELAQPDSILIFPTLSAAKRASREYLTN
                     WDLSWRGFYSMEELRSALILPKQPLLTDEKRLLCLFMVMEESERESFHIYNYGDIVDW
                     GKSFFDFFEELCEECIALSDLEEMANSGAFPMQEWQEIYLQKILNIRSNYQKYINALG
                     FSDTIFYLNADNISVPWQNCDIYYINQYYYSGLEKQQIKALEDSGNRVTIITHGLETE
                     GNAENWKVKDFNLQESWNSLKKKPSIEIRETDNETQMALSFLSWNLEQDCHNCAIIDS
                     TFHLKSYSHYFPEEHFAKPESYSFCEGTIYKMLSAIATGLKATKDTNGYLPIKILANY
                     ISEDWFCRYFYAEQGNLKLDDYFSQLRLELGFLINWDYLYVDYALFLTADLPTLKSLV
                     WEYYNLINAFGKVQNLKELCNLIDSPNCLQLNNLLEDNEKLYTDILPVFWERMANFMA
                     IENLGLVNSWKQIFAEDSIGLNLLDLLLNFLTTGKISYQRNEALTPEWEISNLLDARN
                     RSFSTVAFFQMIEGIIPSSPTPVWLFNESQRAKLGLKTFNDIRNWERYYFCRLLLCSE
                     RAICFSYINQERDISSSSFLGELEELLKNEELDLFKKRKILVSLPDVYNWELVNKTIP
                     ELEDQEICNINKDLTEDFFIIPSDPIADFPMDNQINFSASALIQFLKNPFLWFVENKS
                     KLSIQNWEAEETISYKLFGNIMHTYFSTTLGKLKGIHESHTILETLFGNSKKLEEQLK
                     NVIASEKMIYQIPKNYNAVFLGEILAKKLSESLFFFYQDWLKKQVEGRKFILIPEEDK
                     ISRAEMKHKQLGKVNWGNKEYIIGIRGKADLRIEMENKALIVDFKTGSHDYRQLCIYE
                     WYYYLLDDVSAPNDVSSIFWNILDPTDKVEGVNEEKRNKLKQQILDNLEFCLANGYST
                     ITKATDRQRLQNITRADLISRKGGLK"
     gene            12081..15422
                     /locus_tag="PLG69_03530"
     CDS             12081..15422
                     /locus_tag="PLG69_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPI42612.1"
                     /translation="MTENNCRIICASAGTGKTYRLSVEYLSLILKYYSKNEFALDNIL
                     VLTFTRKATAEIRDRIIKHLELLVSEPETEEEQKKQQELLLNLRKLNKTKEITLSEQE
                     RNLLLSARKELICDQSHLQVMTIDSYTGQIFRNIVRPLKNIEHYEIDTEAVKKIMPFL
                     LDHLMEPDLREKIKNLLSRKVSRSLDEYTKFFCSLIEQRWLFYMITKRQAVHNSSLSG
                     TLMNLNPETEPAEFYEKFLKEMQRIIALVANICSDLGKASLEDYFNKDFRNLAVKGAL
                     TPVAIFNNLKQLNSDEAEKLLYILDKGTIWNKQKISNKKFEAENIELENAQNEAKHHL
                     ADYLIWKLYLPEQREIEELWSSVLERYDNLIYRYKNLTYDDITWFTFETLFSSEPPFL
                     NPEDAVSATEFYQFLSHRTRFLLIDEFQDTSLIQFNILKPIIEEITAGEGSKPFGGLI
                     VVGDEKQSIFGWRGGERDLLLNLKDIFPALGDLTVEALDKSYRCGPTLMEFINQVFMN
                     AELCSLLQEKEMQWDYTHIESVEITKEPGTEIEFCLKNFSTSVANADNEKDIYTDFVE
                     RMVLPALEKDDSGSIAILCRTNEELTKIQQALDISGRASLFQPNRSIIEHHYVAPLLS
                     WLRFLAWGDWMDFIAFLRSDYVLINTKVLKQTLNIISRTLEIQNERQKPVRPDFSTIP
                     VVNEFYQLSWEQENKKATEICREIIDLCLPSKQLSERDYLNLHRFLDILSTRQLTDSD
                     KGISFPDLLTYLKDNANAEDFKQVSINADDSLQLLTIHKSKGLQFKRVFVFCNLSGKH
                     STDSNTLNWALEYEGNTFNRIRDYGISLHYQKILKSSSYANLYLQNQKRKQLEELNNL
                     YVAFTRAEQKLHLYFSYKLKEGWNDYYNTNKDDNLTVALCNAVFDYFKDSTSNERGVY
                     YKKSFLQENKKETSFSSEPPKSNLEQSNSGLKIRHHRPVNWNKLVPVELPAVKDLKTY
                     YLTERPNLIGDLLHFYMSFIIRNTPKEHNYAYYRCLNRYGAIFTVFQIEQLADRCQKV
                     CDSNPYLFTSGWDKIFTEQEILWDSKIMRIDRLMVNTAEKVVLIADYKSGDIYDFNQL
                     ANYKTALSKLSVLKGYNIDTRIVIV"
     gene            15538..15789
                     /gene="purS"
                     /locus_tag="PLG69_03535"
     CDS             15538..15789
                     /gene="purS"
                     /locus_tag="PLG69_03535"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPI42613.1"
                     /translation="MLKAKIYVQLKANVLDPQGKAVTNSLHNLGYTDVIETRISKYIE
                     MSFRNDNREKVSQEVEKICQDLLANPNTETYQYEIISEE"
     gene            15794..16480
                     /gene="purQ"
                     /locus_tag="PLG69_03540"
     CDS             15794..16480
                     /gene="purQ"
                     /locus_tag="PLG69_03540"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HPI42614.1"
                     /translation="MRISVVTFPGSNCDYDTYRVCKSRGHQTKLIWHKDSDLEKPDLV
                     ILPGGFSYGDYLRCGALARFSPIVKEVIAFARKGGLLVGICNGFQILTECGLLPGTLM
                     MNENLHFICQHQYIRVETEASPFTQGIGKGKVMDIPIAHKEGNYYIDPEGLKQLIAND
                     QILFRYCDKQGNINIASNPNGSLDNIAGICNKQRNILGMMPHPERSATETVPTKDGRW
                     IFAAIENALE"
     gene            16452..17159
                     /locus_tag="PLG69_03545"
     CDS             16452..17159
                     /locus_tag="PLG69_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPI42615.1"
                     /translation="MLPLKMLWNKILELLIPPACLVCGNRIDDRYQVICPDCEAKLYL
                     IGEGTCPVCGSENKELPCEVCAEGNFAFDSAISVFRYSGPVRDLIHILKYNGYSSPAG
                     YFALPIAELIESKPVLKNYDYICAVPLHRVRKRERGYNQSDLIAYSVAKLIDMPYFNP
                     VKRRINTLSQTLLSRDHRIKNLAGAFQVKDKNLVAGKKIILIDDVFTTGSTLNEIAKT
                     LRSAGADKICAITVARA"
     gene            17161..17559
                     /locus_tag="PLG69_03550"
     CDS             17161..17559
                     /locus_tag="PLG69_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42616.1"
                     /translation="MNKEFWEIRPETSESEINEMIEKVAKFIAERHLAPAGILLFESF
                     RPLHGIGSQALFFILPVAEIIFDSKKYQQFAIMMEDENNIKKLINRMDELDEIYTRER
                     RQENKLKKQHRKAKRKAFYHKLFKKINKTA"
     gene            17575..>18951
                     /locus_tag="PLG69_03555"
     CDS             17575..>18951
                     /locus_tag="PLG69_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HPI42617.1"
                     /translation="MQYDEKRLTRIVATDCGSTTTKAILIEWIDGEFRQTIRGEAPTT
                     VEAPLNDVTKGVINATQELEELARLKYHNPNIKFMENGEFVIPRKGDVGVDAYVSTSS
                     AGGGLQMMVTGVVASMTGESAERAALGAGAIVMDLIASNDKRMNHEKIERIRELRPDM
                     ILMAGGEDGGTVKHVVEMAELVASADPKPRLGSGYKLPVIYAGNKDAREEIIKTLGEK
                     VDLIITDNIRPKLEIENLLPAREKIHNLFMEHVMKQAPGYNKLMEWTVGPEHEQVPIM
                     PTPAAVGNIMQAIAKEENIEVVGVDIGGATTDIFSVFTKDFIFNRTVSANLGLSYSIS
                     NVLASAGLENIMRWVPFDINESELRNMIKNKMIRPTTIPSLLEELVLEQAIAKEALRL
                     AFEQHKSFASSLKGMQKQRDISEAFSQSVSGETIVNMMTLDLLVGSGGVLSHAPRRNQ
                     TVMMLIDAF"
BASE COUNT         6161 a         3334 c         3663 g         5793 t
ORIGIN      
        1 cctgaacagg aagatttaac tgaatgggaa caacctactc caaatgatac ccgttttcta
       61 ttttctttct atggcgctca accagaaaat ccttcagaat atagcgatcc ggcaaaacgg
      121 tggaacttgc cacccaaaaa gacaatgaaa atcgtagttg cagttttccc cggagataat
      181 aaggaagacc tgaaaaaatc agcaggttgg gctaaagaaa tatacggcca ggcacaagat
      241 ctgattacgg taactcaacc agatacattt cctcattata cacctcctga gccaccagat
      301 attccaaatt tatatgctca attgcaagat gatggtaatc gtttggatgt ttattgggat
      361 aatcgcagtg aattttctta tgatgttaaa acagttagta ctgcaattat tggctggcaa
      421 aatcccgaaa gtagtttatt gatttcaggt cttgatagtg atcctactcc ttatattgca
      481 aataactggg ttgatatacc tgaagaatat agaccgggac cacctcctga gcctggaatg
      541 gaagataaac gctggaatat gaatgctctg gttaatcctt atacagcttc acgtttacgc
      601 aaggattttc agggttacac cttatgggga agaagtggaa gcggaagcca ggaggactgg
      661 gaaatgatta agcgttggga taaggttgat acacctcgtg atcattccga ttacactgta
      721 aatagtgctt ttcctgattt attcagggat tttggaggtt atttaggtat tgatactggt
      781 cttcccaata aaaatgaatg gaatgtagat gtaagtgagt atcataaatt ctaccacttt
      841 gacgaaaatt atcaacttgt tcccaatggt gatacatttt atggttggcc tatttataca
      901 ccagatattg taataaatgg gacaacatat aatgaagtaa ccgactggca atcccttcaa
      961 aactatgcta acagtataat aggacccgat cttgaaacca cccaactttt gcaagcacgc
     1021 attttcaaac atcaagacat cccggatcat atattttatg cactatatga tcctaaatta
     1081 attcctttac agggatttgc ttttcctgga ggtgaaggag cagaagcccc tgatactttg
     1141 gacttgagta acctacggaa agaaagatta gccagacgtt attacaaatc atatattatg
     1201 tatccccgaa aaggcgttga atattatgtt gctttaaccg cttttgatag aggtattccc
     1261 gcaaatgatc tgaattattt ggaaagcgga agggatgctg atgctaatat gaaagtattt
     1321 ttccccggta ctttggctaa ggaaaatatg gataatataa tggttattcc caatccctat
     1381 attggtagaa gcagttttga cggacgcaga gaaaatgatg aaaagggcga taaaagccgt
     1441 cgtatatggt ttattaatct acctaaacgt tgtgatatcc gcatttatac tttagcaggt
     1501 gatcttgttc agaccttgaa acatgatggt gccccggtta ccgatatcat cactatttct
     1561 aaagcaagtt ctacaggtct tgcagcggat ggtatggaag catgggattt actttcaaaa
     1621 aacaaacaga taatagcccc tggtgtttat ctatactccg tagagaacaa agccgacggc
     1681 aaaaacaaag tcggcaaatt tgtcataatt aaatgaggga ggagctgtga agaaagtatt
     1741 aacacttgtc ctgattatta caatagccgg tttaccgctg ataattacag ccaagccatt
     1801 tggtaaaacg ggaacggtag ctttgcagtt tctcaagctt ggtgtggatg ctcgtgccgt
     1861 aggtatggca gaagcgtata cagccgtaac tgatgatatt tcttctgttt actggaatcc
     1921 tgctggtttg gctcctgcct ttgaaaatca ggtttttgtt tcgcatacta attggcctgc
     1981 cagtattatg catgaattcg gagctgccac atataccaat ggggtatcta ctattgcatt
     2041 atatggcagc atattacata tggatgatat ggatataaca gaagaagagc catttggacc
     2101 tactggagag aaattcactt gcagtgacat tgccatagga gcagcttttg ctcagcaatt
     2161 taccaataaa ttctcagccg gactgggaat aaaatatctc cgtgaaaatc tgtatgaatt
     2221 taatgtaaac agctatgcag ttgatgttgg ttcaatgtat aatacgggtt ggagaaatat
     2281 aaaaatcggg atggcaatgc gtaactttgg tcctgacatt cgttttcggg ttgatgatga
     2341 tgaagacggc aaagatgatg aagacccttt tgatttgttt gataatgatg gagatggtct
     2401 tgttgatgaa gatggtattg aattggatag taaaatccct ctcagcttct ctttaggagt
     2461 tagtggcgac ctgatgcgga aaggaaataa ctattggatc gcatctctgc agttggataa
     2521 tgtaatagac cgtttagaga cctggaattt gggaactgaa tataagctgg gaaacatatt
     2581 tttccgctgc ggatatcaat tcaattatga tacaaatggc tttgctgcag gagtgggctg
     2641 gcaagttcct acttcttttg gaatttttaa cattgactat gcctataccg atatgggtga
     2701 tttggctgag tcctttacta aaggtgctca tcgtgtttct ttaaaaatta aatattaaaa
     2761 gaatcaataa agattcagga ggaaataatg aaaaaaatcc tattagtcat cttaacattg
     2821 tctttgcttg tgggcatagt ttttgcccag gaaatgattc ctactcccga taacaagaag
     2881 gcaatggaaa cccctgtccc taaaaaaata ataccgagcg aacgaacagc tccggtatat
     2941 acttttacca aaactccgca ttctctttta acttcttatt atgattatat gataggaagt
     3001 tataacggtt tgcctttacg tgtaattccg tctaatattt taggaggcgg atatttctta
     3061 acctatcatg gacgtagaac ttctacggga acccgtcgta tattttacgc ttatcttgat
     3121 gctaacggtg atgttatcgt taataatgag attacaacag aagcaaataa tgaaggatat
     3181 ggcactgtgg ctgttgatcc ggtttctgga aagccatttt atgcttggca tgcaaattcc
     3241 gatactgatg cggaactgga agtgcagttt accgtagatc aattttatga gggcatttat
     3301 ggcttattta cccctcttgt agatgctatt gataatcctt caactgttta tacttccgat
     3361 ggtaatttta ctgacgataa tgaattcatt tggcccacgg ctgtaattgg tccctctcct
     3421 tatcccgata agagaagaat ctatgtgata gcaagaaact caattactca cacttacggt
     3481 ccttccgaaa atcccaaaat tgcttatgct gattttgatg cttttcaaat tgaacaaaat
     3541 gttcctctta cttggcaata tacctcaatt ccggaaatgg atgaatggaa tgttgatagc
     3601 gagtggcgta gacccttcaa ttccttaact gcagataatg caggaaatct ttattatgcc
     3661 ggttttcatt ttgctacgga agccgatgga tcaaccgata ttgttgagcc ggatgtagat
     3721 gttttccaat gtggaaatta tggagaagga acttggtcgc ggaccagtag ttggggtaac
     3781 ctgcctacat ggaatcctaa taccagtcca actgatacaa ctggtttttt caaaaaccaa
     3841 agcgaattac cttatggtga taatgatatt tactgggaat tattttcttc ggcttcaggt
     3901 catttgaatg ctactgttga cgatttgggt cgtattcatt tccctgctgt ttggagactt
     3961 gttaccaatg atggttattg gtttcctaat ttccaattac ccaaagaaat tatctttgat
     4021 ccttctctgc cggaagattc tcagtttaga attaacgata tctggccccg taaggatcca
     4081 actaacgatg tagatgaaca ttatcagcca tgggatagcc aaccaccttg gggtgttgag
     4141 gactttgttc aagaccctga tacaggtgag tggtttgttg atatgtattt aagctgggaa
     4201 ttcccctatt gggatcagaa tgctcatgat agtgctatgt ttttccattg caataatatg
     4261 aaagttactg aaaataatgg tcatggaatg ttagctatgg tctggcaaaa tagcgcacgg
     4321 gcaaaatggt ataatgctga tggtgatagc gactattctg cttgggcaaa taccccagaa
     4381 atttggattt ctgtttctgg agataatggc aatcattgga gtgaacccat taaactgaac
     4441 aatattgaga cccctgaatt tgcaggcatt aagccaatgt gggtatatcc agccgacaaa
     4501 gttatttatg ttggtgaaag taatggtgag aaagttggta agcttggtct tcttttctat
     4561 gatgatttta cctggggtgc ttttgttatt agtcctggag tcggtcctat gaatgatggt
     4621 ggacgggtaa tgtttacaga actgcaaatc gtctttccca gcgacgcttc agatgatcct
     4681 actgttagcc ctgttaccaa tatgttgaat caaaactatc ccaatccttt caatccggaa
     4741 actaccatca gttttgatat gcccaaaact gctaatgcta atctatccgt ttataatgtg
     4801 aaaggtcagt tggttaaaac acttcataac ggtattgcca attttggaag aaatactgtg
     4861 gtctggaatg gaacagacaa taatggtaac aaagttacca gtggactata tttctatcgc
     4921 cttaccacag atggcaaagt tgaaacccgt aaaatgatgt tgatgaaata agaaaaccct
     4981 tcctcaaaat gtaacataaa aaagctccgg tataccggag ctttttttat ccggaagaac
     5041 gatatcctct tcgttcccca aaatccgtat tacaaaagaa ccaatatgaa acattgtaat
     5101 cggttctttc cgtattcgta atacgatttt tagcgtgaaa tggtacaaat atctttgctt
     5161 attctaaaga tggctgcttt tgcaggattg gcattataca tttattttta tcaaccttgt
     5221 tttttcaaca gcaaccacat aaaaaaagga cagccaataa aggcagtaat tattcctacg
     5281 ggtaattcca tagacaatat atgtttagcg agcagatcac aggcaagaag aaaaatacct
     5341 cccgaccata atgaaagaag atatattttt ttctgtcctg aaggaataaa aaagcgcaca
     5401 atgtgcggaa taattaatcc gacaaagcca attattccgg cataagatac actaattcca
     5461 atcactatag aagtgaggat aaaaatctta cgccttagcg ataaaacatt tattcccaag
     5521 ctggcagcat ataaatctcc gcaacttaaa atatcaatag ctgtggactg gaaaaaaaga
     5581 tagatgagga taataagaga taaccccatc agaattaaaa aacttgtcca ttcaccttta
     5641 gtaaatatat gccccaaatt acccatcagg gttcctaaaa ttagagccgt atcttgctga
     5701 tgaagataca taatcaacga aataccagca gaaaaaaaca ttccggcaat cacaccagca
     5761 atcaataaac ggcttttatc aaatcttcct ttttggtgtg ctagtttcca aaccagcaac
     5821 attgtaaaac ctgcacctat aaaaccacca acaggcatca aaataatcaa tccggatata
     5881 ccaagaagta tactgccaaa agcagaaccg gaagatattc ctaaaatata cggttcggct
     5941 aatggattgg caagcataag ttgatagata gaaccaattc cggctaatgc tgttccggtg
     6001 aaaagagtta acagcaaacg gggaattcta acctgagtaa taatattcgt atccggtttg
     6061 cctaatgtaa gatatgcaat aataatgcct acaccgatta agaaccatag aatcagaatc
     6121 aatatttttt tcatttctct tcccgccatt cagcaaaaat ttttgccagt tctttcattc
     6181 cttcaataca gcgaggagtt gcgcgttgaa gcaaatcggg attgatgtcc ttctcaaagt
     6241 aaattcttct tttttgaata gccggtatat tttgccagcc ctttcggtta attatatttt
     6301 ttagggtatc ctgtgaatag caaatcataa tatccggttt agcagcaata actgcttcag
     6361 gatttattct ggcatagtca cgttccaggg tagaaaaaat attatcgcct ccggcaagtt
     6421 caataagttc acctataaat gaagaatcag aaacgctcat taagggattt ctgtatattt
     6481 ccagataaac cttaggacgg agtttgcctt gtgcatttgc cttaattttt tctatttcct
     6541 cttttaagta atttacgagt tttacggctt cctctttttt ccggataaga gttcctattc
     6601 tttctatgtc tgaaagcatt tcgttaatac ctttaggata aataacttcc acctgaaagc
     6661 ccaaattttt aaggtcataa gctatgtttt cctgctccaa agaactgcaa aaaattagct
     6721 cgggattaag agcaatgatg gcttctttat caatactgct gaaagagcca actatctttt
     6781 tctgctgcag agaaggtgga taattacatt cagcagtaat acctgtaatt tccttttcgg
     6841 catttagagc gcaaataatt tctgccactt ccggagaaag aactacaatg cctttctttt
     6901 gattgtttcc tgtttgctta caggaaaaag caaagagaat aataataatt acagttagaa
     6961 ttcctgattt cttcactgga aaattccttt tttcttttat cctaatttat atttaagttc
     7021 taaacagcag gtcttacaga taaagcaacg agaaaatcac tattccctgg ggaagagaac
     7081 ttactaattc tatttaacag cttatctgtt agtgcgttat cctgcccaaa acaattcctt
     7141 attcatattt ctgttttact cttctaaaaa caagaaatat ttttttcctt aaactaaatt
     7201 taccgcctcc tgtcaagaaa aatgaattgc ctcttcccaa ttgggcataa tttaatccat
     7261 ctgatatatg gaaataatgc ttaatattat ttcccctatt cattaatcct atattgtatt
     7321 aaaaggggtt gatgatggga tatgcctaag tataaagaaa atatgatttc atttggggaa
     7381 tgataagcaa ttttttattc ttgcaaaagt aggaatctgc taaatccgta ttactaacct
     7441 atacggaaag aaagtattgg tattttttcg gcttttgggc ttgacaatct tttcctgtaa
     7501 tttagtaagg tgtttataat agtaattgga atgatgtaaa tataaaagat taagcttaat
     7561 aaagaggtga attattttaa cttccagtaa attatgggat tccatagcat tgattatggg
     7621 tctgctgata agtttggtat taattgcatc cctcacgcag atcatagttg gtggttcagt
     7681 tcttgataca ttcagtaaag taactttacg aattatcaat attctgtttg gaatttccat
     7741 agtgttaatt atcctcatcg tggtaatgga aaatggttct ccggcaacaa ctatggcttg
     7801 gattttagtg ctcatttttt taccggttgt gggctttata ttgtatcttt tttttggcag
     7861 aaattggcga aaaaagcgct tattcagtaa aaaaggatat gccgatacca aattgctgtt
     7921 atcttatgtt cacaatcatc cttccgatag agaaaacaat tggcagaacg acttgactgc
     7981 acgactttat aacttattga aaaataatag taaagctact ttaacggtaa acaacaattt
     8041 aactctatat aacgatacgg gaaaagcatt taaagcaatt ttggaaggca ttaattctgc
     8101 ccaacatttt gtccatctgg aatatttttc cgtttttgcc gatgaaacag gttacacttt
     8161 gcaaaagtta ttgattcaaa aagcactgga aggtgttgaa gtgcgtttta tttatgatga
     8221 tgtaggttgc tggatgctga aaaaatcatt taaacaggaa ttacgcgatg caggagtgga
     8281 atttgttcct tttatgcctg tctggatacc ttttttaaac agtcgtttga attacagaaa
     8341 tcatcgtaaa ctggtaattg tagatggtaa aaaggcatat ttgggaggga tgaatatcgg
     8401 agataaatat ttgggtaaaa aacgctatta tggctactgg cgtgattcct tggcagaaat
     8461 tgaaggaaaa gcagttaatg ctttacaagc aatcttttta gcggactgga attttgtaac
     8521 cggaaaaaat ttgatgcaag aggacttact gcataaatac cttcctgtta tttctcctga
     8581 taaacaaaga tttctaccga tgcaaattgt agctgatggt cctgatagtg attatgacag
     8641 cataatgcaa ttatattttg cagcaataac ttacgctaaa catagcattc ggatttctac
     8701 tccctactta attttaaacg aaagtttact ttccgctcta aaaactgccg ctatcagcag
     8761 tgtaaaagtt cagatactgg ttcctgataa gcccgatcat tttttagttt attgggcttc
     8821 acgcagttat tttcaacaac ttttagatgt cggagtagaa atttggactt atcataaggg
     8881 ttttaatcat tccaaaatat tgattgtgga tgacgaattt ctgctaatcg gaactgccaa
     8941 tatggattta cgcagtttta aacaaaattt tgaactggct gccgcaatat acgataaaac
     9001 tgtttgtgct gaagccatta agcaatttga tgaagacctg ttatccagcg gtaaaattat
     9061 tcctgagaaa ttcagacagc gcagtatgct gcaaaaaaca aaagaatcca tctgccgtct
     9121 ggtttctcca ttgttataac atccgggagg ttaaggtctt aagtcagcag ttatcagtcc
     9181 tcactaccaa ataagtttgg gagcaatgaa aaagttattt cggcagtgac gacttctgac
     9241 ttaaaaaaag gagaaaaagt tatgaagttt atcaactatc ctttatcaga gaatctggat
     9301 acaatagtcc aagaacttgc tcaaccagat tctattctaa ttttcccaac tttaagcgca
     9361 gcaaaaagag cttccagaga atatttaacc aattgggacc tttcctggcg cggtttttac
     9421 agtatggaag aattgcgttc tgctcttatt ttgccaaagc aacctttact gacagatgaa
     9481 aaacgactgc tttgcctctt tatggtaatg gaagaaagcg aaagagaatc atttcatatt
     9541 tataattatg gtgatattgt ggactgggga aaaagttttt ttgatttttt tgaagagctc
     9601 tgtgaagaat gtatagccct ttccgatctt gaagaaatgg caaattccgg agcttttccg
     9661 atgcaggaat ggcaagagat ttatctgcag aagattttaa atatccgcag taactatcaa
     9721 aaatacatca atgctttggg tttcagtgat actatttttt acttgaatgc cgataatatt
     9781 tccgttccct ggcaaaactg tgatatttat tatatcaatc aatattatta cagtggcttg
     9841 gaaaaacaac agataaaagc tctggaggat tctggaaaca gggtaacaat tattactcac
     9901 ggtttggaaa cagaaggcaa tgcagaaaac tggaaagtaa aagatttcaa tttgcaggaa
     9961 tcctggaatt ccttaaaaaa gaagccctcc attgaaatta gagaaacgga caatgaaacc
    10021 caaatggcat tatcttttct ctcctggaat ttggagcaag attgtcataa ttgcgctatt
    10081 attgacagca cctttcactt gaaaagctat agtcattact ttcccgaaga acattttgcc
    10141 aaaccggaaa gctattcttt ctgcgaaggg actatttaca aaatgctttc tgcaattgcc
    10201 acaggactta aagctacaaa agatacaaat ggttatctgc ccataaaaat attggcaaat
    10261 tatatctcgg aagattggtt ctgccgttac ttttacgctg aacaaggtaa cctaaaactg
    10321 gatgactatt tcagtcaact gcgtctggag cttggttttt taattaattg ggattatctt
    10381 tatgtggact atgctttgtt tctaactgca gatttgccaa ccctgaaatc cctcgtatgg
    10441 gaatattaca atttaatcaa tgccttcggc aaagttcaaa atctgaaaga actctgcaac
    10501 ctgattgata gtcccaattg cttacagcta aataacttgc tggaagataa cgaaaaactc
    10561 tatactgata ttttacctgt tttttgggag agaatggcaa actttatggc tattgaaaat
    10621 ttgggcttgg taaattcctg gaaacaaatt tttgctgaag acagcattgg cttaaatttg
    10681 cttgatttac ttttgaattt tcttacaacc ggcaaaatca gttaccaaag aaatgaagcc
    10741 ctaactcccg aatgggaaat tagtaattta ctggatgcaa gaaatagaag tttttccacc
    10801 gttgccttct ttcaaatgat tgaaggcatt attccttcca gtccaactcc ggtttggtta
    10861 ttcaatgaat cacaacgagc aaaattagga ttaaaaacat ttaacgatat cagaaactgg
    10921 gaacgctatt atttctgtcg tcttttactg tgttcagagc gcgcaatttg tttcagttat
    10981 ataaatcagg aacgcgatat tagctccagt tcctttttag gtgaattgga agaactgctg
    11041 aaaaatgaag aactggattt atttaaaaaa agaaaaattc ttgtttctct gccggatgtt
    11101 tataattggg aactagttaa taagacaatt cccgaacttg aagatcaaga aatttgcaat
    11161 attaataaag acctaactga ggatttcttt ataatccctt ccgatcctat tgctgatttc
    11221 ccgatggata atcagattaa ttttagtgca tcagccctta tccaattttt gaaaaatccc
    11281 ttcctctggt ttgtagaaaa taaaagcaaa ctctcaatac aaaattggga agccgaagaa
    11341 accataagtt ataaactctt cggtaacatt atgcatactt atttttctac taccttaggt
    11401 aaattgaaag gaattcacga atcccacaca atattggaaa ccctctttgg taatagtaaa
    11461 aaactggagg aacaactaaa aaatgtaatt gcttccgaaa aaatgatata tcaaatccct
    11521 aagaactata atgccgtttt tttaggagaa atcctggcaa aaaaattatc tgaatccctc
    11581 ttctttttct atcaggactg gctgaaaaaa caggttgaag gaagaaaatt tattctcatt
    11641 cccgaagagg ataaaattag ccgtgcggaa atgaaacata aacaactggg aaaggtcaat
    11701 tggggcaata aagaatatat tattggcatc agaggaaaag cagacctgcg tattgaaatg
    11761 gaaaataaag ctctcattgt ggacttcaaa acaggcagtc atgactatcg tcaactctgc
    11821 atctatgaat ggtattatta tcttctggat gatgtttcgg cgccaaatga tgtttcttcc
    11881 atattttgga atattttaga ccctacagat aaagtggaag gcgtgaatga agaaaaacgc
    11941 aataaactga aacagcagat tttggataat ctggaatttt gtcttgcaaa tggttatagc
    12001 acaattacca aagccactga tagacagcgt ttgcaaaata ttacccgggc tgatttgata
    12061 agcagaaaag gaggtttaaa atgactgaaa ataactgcag aataatttgt gccagtgccg
    12121 gaaccggaaa aacctaccgt ctttctgtag aatatctttc actaatctta aaatattaca
    12181 gtaagaatga atttgctttg gataatattc tcgttttaac ctttacacgt aaggcaactg
    12241 cagaaattag agatagaatt attaaacatt tggaactatt ggtttctgaa ccggaaacgg
    12301 aagaagaaca aaagaaacaa caagaattac tgctcaacct tagaaaactc aataaaacaa
    12361 aagaaattac cttaagtgaa caggaaagaa atttactgct tagtgcacgc aaggaattaa
    12421 tctgtgatca aagccatttg caagtgatga ctattgattc ctacacagga cagatcttcc
    12481 gtaatattgt gcgtccctta aaaaatattg aacattatga aatagatacc gaagccgtaa
    12541 aaaagattat gccctttttg cttgatcatt taatggaacc ggatttaagg gaaaaaatta
    12601 aaaatttatt aagtaggaaa gttagccgtt ccttagatga atatactaaa tttttctgct
    12661 cattaattga acagcgctgg cttttttata tgatcactaa acgccaagca gttcataatt
    12721 cttctttaag cggaacttta atgaatttaa atcccgaaac tgaaccagct gaattctacg
    12781 agaaattcct gaaagaaatg caacggatta tagcattagt agctaatatc tgttctgatt
    12841 tgggtaaagc atctctggag gattatttta ataaggactt tagaaattta gctgtgaaag
    12901 gtgccctaac tcctgttgct attttcaata acttaaaaca acttaattcg gatgaggcgg
    12961 aaaaactact atatatatta gataaaggca ctatctggaa taaacagaaa ataagcaaca
    13021 agaagtttga agccgaaaat attgaactgg aaaatgctca aaatgaagcc aaacaccatt
    13081 tggctgatta tttaatctgg aaattatatc ttcccgaaca aagagaaatt gaggaacttt
    13141 ggtcaagtgt attggaaaga tacgataatt tgatataccg ctataaaaat ttgacctatg
    13201 atgacataac ctggtttacc tttgaaacac tctttagttc agaaccacct tttctaaatc
    13261 ccgaagatgc agtttcagca acggaatttt atcaattttt aagtcataga acccgttttt
    13321 tactgataga tgaatttcaa gatacttccc tgattcaatt caatatatta aagccaatca
    13381 ttgaagaaat tactgccgga gaaggttcca aaccttttgg gggcttgata gttgtaggtg
    13441 atgaaaaaca atccattttt ggctggagag gaggtgaacg tgatttactg ctgaatttga
    13501 aagatatttt cccagctttg ggagatctta cagttgaagc actggataaa agctaccgct
    13561 gtggtccaac tttaatggaa tttatcaatc aggtctttat gaatgcagaa ttgtgtagct
    13621 tactccaaga aaaagaaatg caatgggatt atacacatat tgaaagcgta gaaattacaa
    13681 aagaaccggg aaccgaaatt gaattctgct taaaaaattt ttctaccagc gttgccaatg
    13741 ccgataatga aaaagatatt tacacggatt ttgtagaaag aatggttttg cctgctttgg
    13801 aaaaagacga ttccggttca attgctattt tatgcagaac taacgaagaa cttacaaaaa
    13861 ttcaacaagc attggatata agtggcagag ctagtttatt tcaacccaac aggtctatta
    13921 tagaacatca ctatgtagct cctttgcttt cctggctgcg ttttttagcc tggggtgatt
    13981 ggatggattt tatagctttt ctgcgttcgg actatgtgtt aatcaataca aaagtgttga
    14041 aacaaaccct taatattatc tcccgaaccc tggaaataca aaatgaaaga caaaaaccgg
    14101 taagacccga tttttcaact atacctgttg taaatgaatt ctaccagctc tcttgggagc
    14161 aggaaaacaa aaaggcaaca gaaatttgca gagaaataat agacctctgt ttaccttcca
    14221 aacagctaag tgaacgggat tatttgaatc tacaccgctt tctggatatt ttatccacac
    14281 gccaattaac cgattccgat aaaggaattt cttttcccga tttacttact tatcttaagg
    14341 ataatgccaa tgctgaggat tttaaacagg tttccataaa tgctgatgac agtttacaac
    14401 tgctaaccat tcataaatct aagggcttgc agttcaaaag agttttcgtt ttctgtaacc
    14461 tttccggaaa acatagcaca gatagtaata ccctaaattg ggctttggaa tatgaaggaa
    14521 atacctttaa ccggattcgg gattacggta tcagcttgca ttatcagaaa atcctgaagt
    14581 cctcaagcta cgccaatctc tatctccaaa accaaaaaag aaaacagctg gaagaattga
    14641 ataacctata tgtagctttt acccgcgccg aacaaaaatt gcacctttat ttcagttata
    14701 aattgaagga agggtggaac gattattaca ataccaataa agatgataac ctgaccgtag
    14761 cactttgtaa tgctgttttt gattatttca aagattccac ttctaatgaa cgcggagttt
    14821 attataaaaa aagctttctg caggaaaata aaaaagaaac atctttttct tctgaaccac
    14881 ctaaatcaaa tctggaacaa agcaattccg gactgaaaat tcgccatcac agacccgtaa
    14941 attggaataa attagttccc gtagaacttc ctgcagtaaa agacctgaaa acatactatc
    15001 taacagaacg cccaaacctg atcggggatt tattgcattt ctatatgtcc ttcattatcc
    15061 gcaacacacc aaaagaacat aactatgctt attacagatg cctaaaccgc tatggagcta
    15121 tttttaccgt ctttcaaatt gagcaactgg ctgatcgctg tcaaaaggtc tgcgattcaa
    15181 atccctatct ttttacttcc ggttgggata aaatctttac tgaacaggaa attctgtggg
    15241 atagcaaaat tatgagaata gaccgtttga tggttaatac tgctgaaaaa gtggttctga
    15301 ttgcggatta taaaagcgga gatatttacg atttcaatca acttgcaaac tataaaacag
    15361 ctctatccaa actgtccgtt ttgaagggct ataatattga taccaggatt gtaattgtgt
    15421 agtcaagagg tagagaagtc aaaaaagtgt aaccagagga ctacaaaaca aaagtattga
    15481 ctaaaatatc aagtaaaaag aaaaggataa attgagaaat aaaccaaagg attagcaatg
    15541 ctgaaagcca aaatttatgt tcaacttaaa gctaatgttc ttgatccgca aggtaaggca
    15601 gttacaaatt ctctgcataa cctgggttac acggatgtga ttgaaacccg tatcagcaaa
    15661 tatatagaga tgagttttag gaatgataac agagaaaaag tcagccagga agtagaaaaa
    15721 atttgccaag accttttggc taaccctaat accgagacat atcagtacga aataatttca
    15781 gaggagtaat ttcttgcgta ttagtgttgt tacttttccc ggttccaatt gtgattatga
    15841 tacttacaga gtttgcaaaa gccggggtca tcaaaccaaa cttatttggc ataaggattc
    15901 cgatttggaa aagccagatt tggtaatttt gccaggtggt ttttcctacg gtgattatct
    15961 tcgttgtgga gctttagcaa ggttttctcc cattgtgaaa gaagttatcg cttttgcccg
    16021 aaaaggaggt ttattagttg gtatttgtaa tggttttcag attttaacgg aatgcggttt
    16081 actacctgga actttaatga tgaatgaaaa tttacatttt atttgtcaac atcaatatat
    16141 tcgggtggaa acagaagcca gtcctttcac acaaggcatt ggtaaaggaa aagttatgga
    16201 tattcccata gcgcataaag aaggtaatta ttatattgat cccgagggct taaaacaatt
    16261 gatagctaac gatcaaatac ttttccgcta ttgtgataaa cagggaaata taaacattgc
    16321 aagtaatccc aacggctcat tagataatat agcgggaatt tgtaataaac agcgcaacat
    16381 tttagggatg atgccacatc ctgaacgttc tgctacggaa acagttccca ctaaagatgg
    16441 aaggtggatt tttgctgcca ttgaaaatgc tctggaataa aatcttagaa cttctgattc
    16501 ctcctgcctg tttggtttgt ggaaacagaa tagatgaccg ttatcaggtt atttgtcctg
    16561 attgtgaagc gaagctatat ctaattggag aaggaacttg ccctgtttgc ggaagtgaaa
    16621 ataaagaact tccttgtgag gtttgtgcgg aaggaaattt tgcctttgat tctgccattt
    16681 ctgtttttcg ctattcaggt ccggttagag acctgataca tattttgaaa tataatggct
    16741 atagttctcc tgccggttat tttgctttgc ctatagcaga acttattgaa tccaaaccag
    16801 ttctaaaaaa ttacgactat atatgtgccg ttcctttgca tagagtaaga aaaagagaaa
    16861 gaggatacaa tcaatccgat ttaattgctt attctgtggc taaattgatt gatatgccat
    16921 attttaatcc tgtgaaaaga agaatcaata ctttaagcca aaccctactt tcccgtgatc
    16981 atagaataaa aaatttggct ggtgcattcc aggtaaaaga taaaaacctc gtagcaggca
    17041 aaaaaattat tttaattgat gatgttttca ctactggcag cactttaaat gaaattgcca
    17101 agaccttgcg ctctgcagga gcagataaaa tttgtgccat aaccgttgcc cgagcttaaa
    17161 atgaataagg aattttggga aatccgacct gagacatcag aaagcgaaat caatgaaatg
    17221 attgaaaagg tggcaaaatt tattgccgaa aggcatttgg ctcctgcagg aattcttctt
    17281 tttgaatctt tccgtccttt acacggaatt ggaagtcaag cactgttttt tattcttccg
    17341 gtagcggaaa tcatttttga ttccaaaaaa tatcagcagt ttgccattat gatggaagat
    17401 gaaaataaca taaaaaaact cattaaccgc atggacgaac tggatgaaat ttatactcgt
    17461 gaacgcagac aggaaaacaa gctcaaaaaa cagcatcgca aagccaaaag aaaggcattt
    17521 taccacaaac tatttaagaa aataaataaa actgcttgaa aaagtggagg atatatgcaa
    17581 tacgatgaga aacgccttac ccgtattgtt gctactgact gtggcagcac tactaccaag
    17641 gcaattttaa ttgaatggat agatggagaa tttcgtcaaa ccattcgcgg ggaagcacct
    17701 acaaccgttg aagcaccctt aaacgatgtt accaaaggtg ttattaatgc cactcaggaa
    17761 ttggaagaat tggcacgcct aaagtatcac aatcctaata tcaaatttat ggaaaacgga
    17821 gaattcgtta ttccccgtaa aggtgatgtt ggcgttgatg cttatgtttc aacatcttcc
    17881 gcaggcggtg gcttacaaat gatggttact ggagttgttg cttctatgac aggtgaaagt
    17941 gcagaaagag cagctttggg agccggagca attgttatgg accttattgc cagtaacgat
    18001 aaaaggatga atcatgaaaa gattgaacgc atccgcgaac tccgacccga tatgatttta
    18061 atggcaggtg gtgaagatgg tggcactgtt aaacatgtgg tggaaatggc tgaattggtt
    18121 gccagtgcag accctaaacc ccgtcttggt tccggttata aactgcctgt tatttatgcc
    18181 ggcaataaag atgcccggga agaaattatt aaaactctcg gcgaaaaggt ggatttgatt
    18241 atcacggata acattcgtcc caaactggaa attgaaaatc ttcttcctgc cagagaaaag
    18301 attcataacc tctttatgga acatgttatg aaacaggcac cgggctataa caaactgatg
    18361 gaatggacgg ttggtcctga acacgaacaa gttcctatta tgccaactcc tgcagcagtt
    18421 ggaaatatta tgcaagcaat tgccaaagaa gaaaatattg aagttgtagg tgttgatatc
    18481 ggtggtgcca ctaccgatat tttcagtgtt tttaccaagg acttcatttt caatcgcacg
    18541 gttagtgcca atttgggttt aagctacagc atttccaatg tgttggcttc agcaggactg
    18601 gaaaatatta tgcgctgggt accttttgat attaatgaaa gcgagctgcg taatatgatt
    18661 aaaaacaaaa tgattcgtcc tactacgatt ccttctcttt tagaagaact tgttctggaa
    18721 caggcaattg ccaaagaggc attacgtttg gcttttgaac agcataagtc ctttgccagt
    18781 tccttaaaag gtatgcaaaa acagcgtgat atttcggaag cattctcgca atccgtttcc
    18841 ggagaaacaa ttgtaaatat gatgactctt gaccttttag ttggtagcgg aggagtttta
    18901 tctcatgctc cacgcagaaa tcagaccgtg atgatgttaa ttgatgcttt t
//
LOCUS       DAOPFL010000029        18306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_3374_length_18306_cov_89.022903, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000029 DAOPFL010000000
VERSION     DAOPFL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 18306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18306
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3374_length_18306_cov_89.022903"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PLG69_03560"
     CDS             <1..1142
                     /locus_tag="PLG69_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPI42618.1"
                     /translation="EKFKQDNDFLYVTGLQIPEAIYFGYKGQNGPVEMLFIERSDPEK
                     AIWTGPVMSSEEASKKSGLSQDKIHYLDEFLTILSVYCPVINKLWANIGIQALNKPPT
                     RQLFHLFPIRERYPNINIESLETLITPLRKVKSEWELRQLQRAIDITGKAIIDVWEKA
                     EPSMMEYELEAIMFYRLQRMGVKHWGFAPIVGSGINAATLHYEKNECPIESGEVVLMD
                     VGASYLNYSADITRCFPVSGTFSERQKQVYNAVLDVQKKIIEMIKPGVELSTLNQTAR
                     DLLAKGAIELGLIEHEEDIKKYYMHSISHFLGMDTHDVGGRNAVLEIGNVITVEPGLY
                     IPEEKLGIRIEDDVLVTENGYCVLSQNIPKEISEIEEIRLQAMAK"
     gene            1142..1621
                     /gene="coaD"
                     /locus_tag="PLG69_03565"
     CDS             1142..1621
                     /gene="coaD"
                     /locus_tag="PLG69_03565"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPI42619.1"
                     /translation="MSKAIYPGTFDPFTLGHLNILEKACHIFDEVILAVAEYSGKENL
                     FNLQERYELCKKATEHIPNLVVSTFQGLFVDYAKSQNCKIMIRGMRAVSDFEYELSLA
                     ITNKKLNPEIETIFLVPSLKYMYLSSSMVRQLADLNGPLEDYVPACVAEAMRKKYVS"
     gene            1626..3002
                     /gene="dnaB"
                     /locus_tag="PLG69_03570"
     CDS             1626..3002
                     /gene="dnaB"
                     /locus_tag="PLG69_03570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPI42620.1"
                     /translation="MAKLSEADKLNKAQTERALPADINAEAALLSAMLIDSDVVSKGI
                     EKIKEEYFYRNAHKLIFRTMQELFFEGIEIDQLTLINRLERNNLLEKVGGIPYINEIA
                     DFVVSSANFDYHLNIVTDQALLRHLILASNGIIENCYTAAKPVKTIVDEAEQAIFAIA
                     ELPSHQGFVRFDQVSQAVLKNIDDIASTKVSVIGVSSGFADLDRFTGGFRPGQFIIIA
                     ARPAMGKTSLALNIAANAAVNLNKKVAIFSMEMAADEVIMRMFSSASEVRMDGMLRGY
                     GMNEEKLIRIMQASEVLSAKHIYIDDSGTNTPLDIRAKTRRLAAEIGGLDLVLIDYLQ
                     LMTLTREKDNRQQEISEISRALKILAKDMKIPVVALSQLNRAAEGREDKRPKLADLRE
                     SGAIEQDADLVMFIYRDEYYYPETTQKPGIAEIIISKNRHGATGNLELGFEKEFTLFR
                     PLETKYEQ"
     gene            2992..3741
                     /locus_tag="PLG69_03575"
     CDS             2992..3741
                     /locus_tag="PLG69_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI42621.1"
                     /translation="MSSNLFAGIGEYTIFVGKCLGKIPSLFKRYQEFLIQFKRIGFDS
                     LFLILLTAAFTGLVTALQAVYQSKGYVPLNLLSVLIGKSTMIELAPVLTALVLTGKVG
                     ASIAAEIGTMRVSEQIDALESMNIDPYEFLYMPRILAGVLAFPLVTVFANAVGIICAW
                     YFSWLRYGIHYYTFFNNMRSYFIPSDLWGGLAKSFVFGFIITSFACYHGDRCFGGAEG
                     VGKATTSTVVYSSVAILVMDFIVAWFLFGGY"
     gene            3742..4797
                     /locus_tag="PLG69_03580"
     CDS             3742..4797
                     /locus_tag="PLG69_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HPI42622.1"
                     /translation="MKVWKLIPLLFIVFICSCGKENKKQESLTYKHKLVVYATDEFRK
                     SGLEHSIVPDFQKKHNCQLEVILFRNTAELSKAVKNRDNYGKYDLAIGIDNSFAFSET
                     LAVNFVPPESFDKELLIQETVFDPYLRFIPYAYSNLSLIYNTSLVKTPPQSFGELQDA
                     KYLSQIAICDPQTSGLGRSMLFWSVALFGTQGYEHLWKSLRKNIYKTYSNYNEALETL
                     KRGECSFIIGYNTTPAFLQELDPLNRNFEVSMLKEGSFQYIEAIGIHRGTKKSSLCYK
                     FVAYFLSSEAQKMVVYKLGMFPANRKTLLPMHFSAIPFISYSVNDRLSQATIQEQINA
                     WLDFWERLFGYQVAKGL"
     gene            complement(4837..6864)
                     /locus_tag="PLG69_03585"
     CDS             complement(4837..6864)
                     /locus_tag="PLG69_03585"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPI42623.1"
                     /translation="MKTTYAEIKNRILDCKKPYKTLLQRLHSLDILSDMPYAKFFQDL
                     QNLCLQSDKFAAFREKDPQLKTIPLEQLSTWHNDYYSALGNYDNCLGNPDYAVKLYGE
                     EMGRFLSALYYQFMLMYGNQATNNYLQLEENLRLFFILYDKAISGNTDYADWLKEYRK
                     IVIENHKTKLYWKLYQNYYPEANINYDLIMNSDLNDVRYLYRYGMFITDASIKMAQFI
                     NNYPEKQLNDIARYIVQCWIDGFIRAGKNYRIKKTATIIVPCGMELLGRLVHQELEKI
                     GIQGQFSVPYKIYENKQFNYDHRFDSALILDRDYTDMVINVYAEVIDDIQELIKGDAG
                     PVYVELFGETPFSPIVKNSVLTLNKEQQELSREMMSKTSQIYYQYYKRDQASFCIIAF
                     PSPEIGEQFPDIFADTLKINLLDSNHYAEIQQKIIDVLDKADYVNVKGKAGNFTDINV
                     KLHTLQNPEKETNFENCVADVNIPVGEVFTSPVLKGTNGTLQVEDIYLGSLRYFNLKI
                     DFEDGWIKDYSCTNFADPEEGKKYIYENLLLPHKSLPIGEFAIGTNTLAYQMARKYNI
                     LPLLPILIIEKMGPHFAIGDTCYSHEEDSPHPSFVNGKEMIAVDNEKSITRKEDPLNA
                     YLQKHIDITLPYEMLGSITAVTKNGEKFDIIRDGRFVVPGTEELNIPLQEG"
     gene            complement(7471..7941)
                     /locus_tag="PLG69_03590"
     CDS             complement(7471..7941)
                     /locus_tag="PLG69_03590"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPI42624.1"
                     /translation="MAAYDLNHLIGKNGKLPWKITEDLKRFKAITMGHPVIMGKNTWN
                     SIGKPLPGRQNIVLSKDRNFQPQGAIVAHSLEEALKMVEGQEAYIIGGESVFKQFLPL
                     ADKMLITLIHSEFEGDTYFPYFSEEDWVLQESSSLLSSSGYELCLQTYIRKNLL"
     gene            complement(7953..8690)
                     /locus_tag="PLG69_03595"
     CDS             complement(7953..8690)
                     /locus_tag="PLG69_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI42625.1"
                     /translation="MEPVISIRNLIAKYGDLTVLDDISVDIFPGEITVILGSSGCGKT
                     TLLKNILRLVEPVSGSVKFWGEEILDLDDIQMEKILRKLGVLFQSGALLNSISVYENI
                     SIPLELHTKLPRPLIDRIIQVKLNLVNLNEALYKYPSELSGGMKKRAALARAIALDPQ
                     ILFCDEPSAGLDPLTAASLDELILSLKKQLKMTIVVVTHELASIHRIADKIIFLEEGK
                     MLFNGTLEEAKKAGIPQIDTFFEVGRF"
     gene            complement(8693..9769)
                     /locus_tag="PLG69_03600"
     CDS             complement(8693..9769)
                     /locus_tag="PLG69_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HPI42626.1"
                     /translation="MSCKIVNNILYLEGSLNAQTVPETFIEVNSLLKHQSPNTIDLSN
                     VNVLDSAGVAFLDELNTRLSAEGILLFQGATPEIQEVIDSFSSLKIKVPAPERKMGFF
                     EKIGDSLVLAGQNFYNVMLLASEVFYWAVIGIFSRKGQRKGSFTIQCSLLGSQALPIL
                     SLLSFIVGFILSLQASVQLANFGANVFIADLMAFAMVREIAPLITAIIVAGRSGSAIA
                     SEIATMQVTEEIDALKTMALSPVRYVVVPKFHAITVVMPILVMFSILVSEIGGGLIAI
                     DYLDLSVYTFVQRSINVLTLKDLVTSFGKSVIFAWTIVIIGAYCGFQVKGGAEGVGKA
                     TTSSVVASIFAVIIWDAIFSLLYL"
     gene            complement(9778..11967)
                     /locus_tag="PLG69_03605"
     CDS             complement(9778..11967)
                     /locus_tag="PLG69_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HPI42627.1"
                     /translation="MLKRKCLYIFSGFLAILLLLTGCLQKVERTPVNYYVLEYQPGTE
                     KPELRRTTNTGKNLEVLDTVLNRTYDRSQIVVKENFYNVKFLQTDVWATRLRDAIPNL
                     IVQRLKAYNIFGTVSRGEQLEKNPHYFLETKVYNIEKIAGTEPRAYLNMEFVLRDSTS
                     ERIVFSHKGERTMELIDPSMIYLVQAFNEMIMEETDLFAAKCNLYFSGLPVESKSLAA
                     STSPIARYVYEEMIARETDFDFGELMVITKTQTEEQIRYTIEELDSLNTVISTDELIM
                     GVPALLTPGNYRVIIGEMGDLIIPVKILPRQRTVVKPNWGELKIVVMDESKARVRMGY
                     DLWKKNTDGEGYKIYSGGMFSMGEDEVGAVDKLWILPPGSYLVKLGGGSWSDLRDFAT
                     VTLAEGEKKTLTMIVDPAAEGNVLIGAGVFADEDIGLGSKRIHRGAIHGNISLTSNNN
                     VDKDKPTTSASLSGQFDNSIDTHELLKPFQFTTRSIYDIGLNLTSNQDLVFSPDDYSL
                     KSVLLFYPLEERKFLKNFAFYGRMNLNTHIFEENTFFSENKNIMLQNSAGDTISLLLN
                     QSELKTKIAFYPLRLKEGIGLTYQINFSSNARTSLRVGYGWAQDYNKNSYVFDKTVTS
                     SLDGVNYEFERYKEEPNSSSKGIESTVILSALNLLKFVSINSTLDVLFRMGEPDHSYS
                     LENENRINFRLFRNISLDVKFNISYDEAKKPWTVYDYTTFLRLSLFY"
     gene            complement(11976..13208)
                     /locus_tag="PLG69_03610"
     CDS             complement(11976..13208)
                     /locus_tag="PLG69_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPI42628.1"
                     /translation="MVSKAAKIRLGIFLIIGAILIILFAIVVAGSRLVEKKDIYYIEF
                     EDYSVSGLQVGSSVNYRGIKIGRVEDVKINPKDVTKIIITISVNRGTPIKEDTEAVLL
                     LSGITGLKNVELRGGTNEAKLLKPKSYIKTGTTVLEDISERAKSIVDKIDMIAANLNA
                     ITGEENRNNISTILAQTSLILKDTHENLATTMQSISRIANNTADLTEELSKDLSAVTE
                     NLTKNLDAITSSATSNIQNVSETSQASLISVTNNVNQQLEELTAKLETSLTQLTNDGS
                     ALIREANLKVSTVGEHSDQMILETTREILTISNNINRALDQVNAILYTPGFDSLMTNL
                     GKLSGELSRTDLKSMVTELSTTIQKTGTLVSNLNRVVTRGQGDLLEILSTLTETSENL
                     NEFSRQIADTPAILLRGN"
     gene            complement(13210..14604)
                     /locus_tag="PLG69_03615"
     CDS             complement(13210..14604)
                     /locus_tag="PLG69_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPI42629.1"
                     /translation="MSTPFIRLMKQEGIRDEVIRTFEAYYQKLVKGEKGLIGEDAIRP
                     PSENNLIDYQQIKHLSRTSILKNTVVIKLNGGLGTSMGLSKAKSLLPVKNNLNFLDII
                     SRQVLTLRSLSGYDVLLLFMNSFNTEADTLKYLEKYPDLGKQDLPLSFLQNKFPRIRQ
                     DTLMPYENKETKLMWNPPGHGDIYTALGDLLDKMIAKDYRYAFVSNADNLGAVVDTSI
                     PAYMENNNIPFVMEVCLRSPMDKKGGHLCEDKSGQLLLREIAQCPEEDLPRFQDIDYY
                     KYFNTNNIWIDLRALEWQMIANEGLMLLPLIVNPKVVDGTPVYQLETAMGSAISVFNN
                     SKALVVSRERFVPVKKTNELLALWSDAYELNEQYQIVLKRGVEKAPVIELDEKYYGKI
                     EDLQKRFSKGIPSLSECKELKIDGDVSFGEDVICEGKVTLKASSPVFVQNCLLSGEVN
                     LDSAINKSKDRTGG"
     gene            complement(14607..16616)
                     /locus_tag="PLG69_03620"
     CDS             complement(14607..16616)
                     /locus_tag="PLG69_03620"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPI42630.1"
                     /translation="MPNRKYEVHLPAELPPEPTFEPNIRRAPDRGLDLSKKDIALALK
                     NALRYIPKELHSVLAPEFLQELKTRGRIYGYRYRPQGHIYGKPIDEYKGITPARAIQV
                     MIDNNLDFDVALYPYELVTYGESGQVCQNWMQYRLIKQYLEIMTENQTLVVASGHPLG
                     LFPSRPEAPRVISTNGLMVGKWDNPIEFKRLTALGVANYGQMTAGGWMYIGPQGIVNG
                     TYITLLNAGRKYLGIPEDKNLAGVLYVSSGLGGMSGAQSKAIEIAGGIGIIAEVDKSR
                     IETRYNQGWVSKVSSNLAEIFNWVDEARHTQKPLSIAYYGNIVDLLEYIDQHNIKVEL
                     LSDQTSCHAVYEGGYTPAGITYEEGRKLLAENIELFKQKVDASLRRHFNVLKNLTAKG
                     SKFWDYGNSFMASVFESGITEIARNGINTNEGFIWPSYVEDIMGPICFDYGYGPFRWI
                     CLSRKDEDLHKTDLAAISLIDPNRSAMDRDNYYWISTAEENKLVVGTKARILYADEES
                     RIKLGLKFNEMVRKGEIGPVMLGRDHHDVSGTDSPFRETANIYDGSNLMAEMATHCFA
                     GNIARGMTMVVLSNGGGVGVSRCINGGNGIVLDGSERMDEVVKSGLSWDVMGGIARRA
                     WAQNEGAIKTGTAWNEKHSAEGNITLAEKVDEEMVKYLVNKEFGA"
     gene            complement(16632..17912)
                     /locus_tag="PLG69_03625"
     CDS             complement(16632..17912)
                     /locus_tag="PLG69_03625"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPI42631.1"
                     /translation="MKDTENILALLKQEVSPALGCTEPAAVALAGAYASSVLKDTIIS
                     ADLIVSPNILKNGMGVGIPNTNLMGLEIAFALGILVAEPEKGLEVLLDLNAETINAGK
                     QMLAEKRINTKMKETEEKIWIEASLKTAKSESKAILRWRHNWLYHWEKDKHILQHNEI
                     GAEEAPRLISPSAFTLKDYYDFCRDTDIDKLELIDLGLILNRRIADFGLSNKSGINVG
                     KMIMEQINSGLLGDDIHHYAMALTAAATDARMSGCNLPVISNSGSGNQGLAITLPIIA
                     VAEKLGSNQEELIRALAMGHLVSVHIKQKIGILSCICGCLSASAGAASGIVMLLKGTY
                     PQIEYAIKNMIADTAGMVCDGAKEGCSLKVATTTSAAVQAALLAKTGICVSSNDGIIT
                     DSIEGTIDNLAYFVNKGMQDADSTILNIMLNKIK"
BASE COUNT         5554 a         3678 c         3255 g         5819 t
ORIGIN      
        1 tagaaaaatt caaacaggat aatgatttcc tctatgtaac cggtttacag attccggaag
       61 caatctattt tggctacaaa ggccagaacg gacctgtaga aatgcttttt attgaacgca
      121 gtgatccgga aaaagcgatt tggacaggac ctgtaatgag cagtgaagaa gccagtaaaa
      181 aaagcggttt gtcacaggat aaaattcatt atctggatga gtttttaact attctttccg
      241 tttattgtcc cgttataaat aaattatggg ctaatatcgg aattcaggca ttaaataaac
      301 cgcctacaag acaacttttt cacctcttcc ctatccgcga acgctatcct aatataaaca
      361 tagaatccct tgaaacactg ataactcctt tgcggaaagt gaaaagtgaa tgggaattga
      421 ggcagttaca aagggcaata gatataaccg gtaaagctat tatagatgtt tgggaaaaag
      481 cggaaccctc aatgatggaa tatgaactgg aagcaataat gttttaccgt ttgcaaagaa
      541 tgggagttaa acattggggt tttgctccta tcgtaggcag tggaatcaat gcggctactt
      601 tgcattatga gaaaaatgaa tgccctattg aaagcggaga agttgtttta atggatgtag
      661 gtgcttccta tttaaattac agtgcagata taacccgttg ttttcctgtt agcggaactt
      721 tcagtgaacg ccagaaacag gtttataatg ctgttttaga tgtgcagaaa aagattattg
      781 aaatgattaa gccaggtgta gaactttcaa ctttaaatca aacagcacgc gaccttttag
      841 caaaaggagc aatagaactc gggttaattg aacacgaaga agatattaaa aaatattata
      901 tgcacagcat cagtcacttt ttgggaatgg atactcatga tgtaggaggt cgtaatgctg
      961 tgctggaaat aggtaatgta attactgttg aaccgggact ctacattccg gaagaaaaac
     1021 ttggcatccg tattgaggat gatgttttag taaccgaaaa cggttattgt gttctttccc
     1081 aaaacattcc caaggagatt tcggaaattg aagaaatccg cttgcaggca atggctaaat
     1141 aatgtctaaa gcaatctatc ctggaacttt tgatcccttc accttgggtc atttgaatat
     1201 actggaaaag gcctgccaca tttttgatga agtaattctt gctgttgctg aatatagcgg
     1261 caaggaaaat ctgtttaatt tgcaggaacg ctatgaattg tgtaaaaaag ccactgaaca
     1321 tatccccaat ttagtagtca gcactttcca gggattgttt gtggattatg ccaaaagtca
     1381 gaattgcaaa ataatgattc gcggaatgag agctgtctct gattttgaat atgaactttc
     1441 tctggcaatt actaataaga aacttaatcc ggaaatagaa actatctttt tggttccttc
     1501 tttaaaatat atgtatcttt catcttcaat ggtccgtcag ttagccgatt taaacggacc
     1561 tttggaggat tatgttcctg cctgcgttgc agaagcaatg agaaaaaaat atgtgagcta
     1621 aagttatggc aaaattgagc gaagcggata aattgaataa agcacagacg gaaagagctc
     1681 tgcctgccga tattaatgct gaagcagctt tgctttcagc tatgctgatt gacagtgatg
     1741 tagtcagtaa aggcattgaa aagataaaag aagaatattt ttatcgtaat gcacataaat
     1801 taatcttccg cactatgcag gagctctttt ttgaaggaat tgagattgac cagttgactt
     1861 taattaaccg tctggaaaga aataaccttc tggaaaaagt aggaggaatt ccttatatta
     1921 acgaaattgc ggattttgtg gtttcttctg ccaattttga ttatcatctt aatatcgtaa
     1981 ccgaccaggc attattgcgt catttgattt tagcttccaa cggaattatt gagaattgtt
     2041 atactgcagc caaacctgtt aaaaccattg tagatgaagc agaacaagca atttttgcca
     2101 ttgccgaatt acccagtcat caaggttttg tgcgttttga tcaagtcagc caggcagttc
     2161 tgaaaaatat agatgacatt gcctctacta aagtttccgt tatcggcgtg agtagtggtt
     2221 ttgccgattt agaccgtttt acgggtggtt tcagacctgg acaatttatt attattgctg
     2281 cgcgtccggc aatgggaaaa acctctttgg ctttaaatat tgcagctaat gcagcagtaa
     2341 atttgaataa gaaagttgct atcttctcta tggaaatggc tgccgatgaa gttattatgc
     2401 ggatgttcag ttctgcttcc gaagttagaa tggatggtat gttacgcggt tatggaatga
     2461 atgaagaaaa gcttattcgc attatgcaag cgtcggaagt gctttctgcc aaacatattt
     2521 acattgatga ttcaggaacc aatactcctt tggatattag agctaaaacc agacggctgg
     2581 cagctgaaat cgggggttta gaccttgttt taattgacta tttacaattg atgactctca
     2641 cccgcgaaaa agataaccgg cagcaggaga tatctgaaat atcaagagct ttaaagattt
     2701 tagccaaaga tatgaaaatt ccggtggtgg cactttctca acttaataga gctgcggaag
     2761 gcagggaaga taaaagaccc aaactggcgg acttacgtga atcgggagct attgaacaag
     2821 atgccgattt ggtaatgttt atctaccgcg atgaatatta ttatcctgaa acaacacaaa
     2881 aaccgggaat agcagaaata attatcagta aaaacagaca cggagctaca ggaaatctgg
     2941 aactgggatt tgaaaaggaa tttacccttt tccgtcctct tgaaacaaaa tatgagcagt
     3001 aatctttttg ccggcattgg agaatatact atctttgtcg gtaagtgttt gggcaaaatt
     3061 ccctcgctgt tcaaacgcta tcaggaattt ctaatccaat ttaagcgtat tggttttgat
     3121 tccctttttt taattttatt aactgctgca tttacaggtc tggtaactgc attgcaggca
     3181 gtttatcaat ccaagggtta tgtaccctta aatcttttaa gtgtgctcat tggcaaatcc
     3241 acaatgatag aactggcacc ggttttaact gccttagttt taaccggaaa agttggagct
     3301 tccatagcag ctgaaattgg cacaatgaga gttagcgaac agattgatgc tttggaaagt
     3361 atgaacattg atccttatga atttctttat atgcccagga ttcttgctgg tgttttggca
     3421 tttcctttag ttactgtttt tgccaatgct gtaggaataa tttgtgcctg gtatttttcc
     3481 tggttgcgtt atgggattca ttattacact tttttcaata atatgcgcag ttattttatt
     3541 ccttcagacc tttggggcgg cttagccaaa tcctttgttt ttggttttat aattacaagt
     3601 tttgcctgtt atcatggaga ccgctgtttt ggcggagcgg aaggagttgg taaagccaca
     3661 acatctacag tggtatattc ttcagtagcc attttggtaa tggatttcat tgttgcctgg
     3721 tttttgtttg gaggatatta aatgaaggtc tggaaactaa tacccttact gtttatcgtt
     3781 tttatatgct cttgcggaaa agaaaacaaa aaacaggaaa gtttaaccta caaacacaaa
     3841 ctggttgttt atgctactga tgaatttcgt aaatcgggtt tggagcattc cattgttcct
     3901 gattttcaaa aaaagcataa ctgccagctg gaggtaatcc ttttccgtaa tactgcagaa
     3961 ttgagcaaag cagttaaaaa cagagacaat tatggaaaat atgatcttgc tattggaatt
     4021 gacaacagtt ttgccttttc tgaaactctg gcagtcaatt ttgtgccacc ggaatcattt
     4081 gataaagagc ttttaattca agaaaccgtt tttgaccctt atttacgctt cattccttat
     4141 gcttatagca atttgtccct aatttataat acttctttag taaaaactcc tcctcaatca
     4201 tttggcgaat tgcaagatgc caaatacctt tctcaaatag caatttgtga tcctcagacc
     4261 tcaggtttgg gacggtctat gcttttttgg agtgtagctc tatttggaac acaaggttat
     4321 gagcatttat ggaagagctt acggaaaaat atctataaaa catattctaa ctataatgaa
     4381 gcactggaga ccctgaaaag aggtgaatgt tcttttatta ttggttataa tacaacccct
     4441 gcctttttac aggaacttga tcctttaaac cgtaattttg aggtttcaat gctgaaagaa
     4501 ggtagctttc aatatattga agcaatcgga attcatcgtg gaactaagaa atcctcttta
     4561 tgctacaagt ttgttgctta ctttctttcc tccgaagcac aaaaaatggt agtttacaaa
     4621 ctgggaatgt ttcctgctaa tcgtaaaacc cttttgccaa tgcatttttc cgccattcct
     4681 tttatcagtt acagtgtaaa tgatcgcctt tcacaagcta ctattcaaga acagattaat
     4741 gcctggctgg atttttggga acgccttttt ggataccagg tagctaaggg cttatagtaa
     4801 aaacgctgtc ctcctcgttc attggttacg aggacatcaa ccttcctgaa gagggatatt
     4861 taattcttca gtgccgggaa caacaaatct gccatctctg ataatatcaa atttttctcc
     4921 gtttttagtg acagcagtta tggagcccag catttcgtag ggcaaagtaa tatctatgtg
     4981 tttttgcaga taagcattaa gcgggtcttc tttacgagta atggattttt cgttatctac
     5041 agcaatcatt tctttaccgt ttacaaaact gggatgggga ctatcttcct catggctgta
     5101 acaggtatct ccaatagcaa agtgaggtcc cattttttct attataagaa ttggcagaag
     5161 aggcagaata ttatattttc gtgccatctg ataagccaaa gtgtttgtgc cgatagcaaa
     5221 ttcaccaatc ggaagtgatt tatggggcaa aagcaggttt tcataaatat attttttccc
     5281 ttcttccggg tctgcaaaat tagtgcagga ataatccttt atccatccgt cttcaaagtc
     5341 aattttcaaa ttaaaatagc gtaaggaacc caaataaata tcttccacct gtaaagttcc
     5401 gtttgtacct tttaacacgg gagaagtgaa aacctcacct acaggaatat taacatctgc
     5461 cacacaattt tcaaagttgg tttccttttc tggattttgt aaggtatgca atttaacatt
     5521 gatatctgtg aaattacccg cttttccttt cacattcaca taatcagctt tatcaagcac
     5581 atcaattatc ttctgctgaa tctccgcata atgattactg tccaaaagat tgattttcaa
     5641 ggtatcggca aaaatatccg ggaattgttc accaatttcc ggtgaaggaa aagcgataat
     5701 gcaaaaactg gcttggtctc gtttataata ttgataataa atttgagagg tcttggacat
     5761 catttcccgg gaaagttcct gctgttcttt gtttaaagtt aaaacactgt tttttactat
     5821 cggagaaaaa ggtgtttcac caaaaagttc tacataaaca ggaccggcat cacctttaat
     5881 cagttcttga atgtcatcta tcacttcagc ataaacatta attaccatat ctgtatagtc
     5941 cctatctaaa atcagggcac tatcaaaacg gtgatcataa ttaaactgct tgttttcata
     6001 tatcttgtaa ggaacagaaa attgcccttg aatgcctatc ttttccagtt cctgatgcac
     6061 aaggcgtccc aaaagttcca ttccacaagg aacaataata gtagctgtct tttttatccg
     6121 ataatttttt ccggctctaa taaaaccgtc aatccaacac tgaactatat aacgggcaat
     6181 atcattaagt tgtttttccg gatagttatt gatgaattga gccattttta tgcttgcatc
     6241 agttataaac attccatagc ggtaaagata gcgcacatca ttcaggtcgg aattcataat
     6301 aagatcataa ttgatatttg cttccggata gtaattttga tataatttcc aatagagttt
     6361 ggttttatgg ttttctatca caattttccg atattccttt aaccagtccg cataatcagt
     6421 atttccgcta atagctttgt cgtataagat aaaaaacaat cgcagatttt cttccaactg
     6481 taaatagtta ttagttgcct gattcccgta cataagcata aattgataat agagagcgga
     6541 aagaaatctg cccatctctt caccatacag ttttactgca taatcaggat ttcccaggca
     6601 attatcgtaa tttcctaaag cagaataata gtcattgtgc caagtggaaa gttgctctaa
     6661 agggattgtt tttaattggg gatctttttc ccggaaagca gcaaacttgt cactctgcaa
     6721 acaaagattc tgcaaatcct gaaaaaattt ggcataaggc atatcagata aaatatcaag
     6781 tgaatgaagc cgttgtaaca atgtcttata tggcttttta cagtcaagga tgcggttttt
     6841 gatttctgca taagttgttt tcatattggt ttccttatta tagtatattc tgggagcaaa
     6901 atacgttcac cctttttgaa aatttatcta ttttggaggc cagaaaatgt gtcaagcaca
     6961 attccatctt cttccctact ccctactcaa ataccacttt acattctcag atacacaacc
     7021 caaactttgg atgggtttag gttgagtgga tgatgggtgg atgatgagtg gggagtaagg
     7081 aagcacttgt aatggtaata atggcaataa attgagcgcc attttctgtt ttaagtcata
     7141 atttttaatt cggaaaccaa ttatagctcg gttgtaagta aaatagtgcg tggagtgtgt
     7201 tttttaggaa ggcaggtgaa aattatgagt gaaaaaccac atagtattaa aagcgtgaat
     7261 ttaacgaggg gcttacgcca ccttctacat cattgtgtcg ccctgacggg cttttggaac
     7321 caggaaaggt aaaaatataa gatggaaaaa aaagtcgggg ctttcgtttt aacgaggggc
     7381 ttacgccacc ttctacatcc ttgtgtcgcc ctccgggctt taatgaaatc tcaaccttct
     7441 caaccctctc aaccctctca tcttttttta ttagagaaga tttttcctga tataggtttg
     7501 cagacataat tcatagccgg aagaagaaag cagagaggaa ctttcctgca aaacccaatc
     7561 ctcttccgaa aaataaggaa aataggtgtc gccttcaaat tcggaatgga taagagttat
     7621 cagcatttta tcagccaggg gtaaaaattg cttgaacaca ctttctccac cgataatata
     7681 ggcctcttgt ccttccacca tttttaatgc ttcctccaaa ctatgagcaa caatagcacc
     7741 ttggggttga aagtttcggt ctttgctcag cactatattc tgtcttccgg gtaatggttt
     7801 tccgattgaa ttccaggtat tttttcccat aataacggga tgccccattg ttatagcttt
     7861 gaatctttta aggtcttcgg taattttcca gggcaatttc ccatttttac caattagatg
     7921 gttcagatca taagcagcaa taattataat actcagaatc tgcctacttc aaagaaggta
     7981 tcaatttgcg gtattccagc ttttttggct tcttccaaag ttccgttaaa aagcatctta
     8041 ccctcttcca gaaagataat tttatcggca attctatgaa tagaagccag ttcatgagta
     8101 acaactacaa tggtcatttt taattgtttt tttaagctta aaataagttc atccaaagaa
     8161 gcggcagtta atggatcaag tcctgcagaa ggttcatcac aaaaaaggat ttgaggatca
     8221 agagcaatag ctcgggctaa ggcagctctt tttttcattc ccccggaaag ttcggaagga
     8281 tatttataca gtgcttcgtt taagtttacc agatttaatt ttacttgaat tattctatca
     8341 ataagaggtc ggggcagttt tgtatgcaat tccagaggaa tactgatatt ttcgtaaacg
     8401 gaaatggaat tgagcaaggc accgctttgg aataagaccc ccaatttacg caaaatcttt
     8461 tccatttgaa tatcatcaag gtctaatatt tcttcacccc agaatttaac gcttccggaa
     8521 accggctcta ccaggcgtaa aatatttttt aaaagagttg tttttccaca accgctactt
     8581 cccaaaataa cggttatctc cccaggaaaa atatccacgc taatatcatc caacacagtg
     8641 agatcgccat atttggcaat caaatttcta atgctgatta ctggttccat aattataaat
     8701 agagtaagct gaaaatagca tcccaaatga tgacagcaaa gatggaagca acaacagaag
     8761 aagtagttgc cttgccaaca ccctctgctc cacccttaac ttgaaaacca caataagcac
     8821 caattataac aattgtccag gcaaaaataa cactcttgcc aaaactggtt accaaatctt
     8881 tcagggtaag aacattaatg gagcgttgaa caaaagtata aacgctcaaa tccaaataat
     8941 caatggctat aagtccacct ccaatttccg aaaccaaaat ggaaaacatc accagaatcg
     9001 gcatcacaac agtaattgca tgaaacttgg gaacaactac atagcggaca ggggacaaag
     9061 ccatagtttt taaagcatca atttcttccg taacttgcat tgtggcaatt tcggaagcaa
     9121 tggctgatcc acttcttcca gcaacaataa ttgctgtaat taaaggagca atttcccgca
     9181 ccatagcaaa tgccataagg tctgctataa aaacattagc accaaaatta gccaattgca
     9241 ccgatgcctg caaggaaagt ataaaaccca cgataaaaga aagcaaagat aaaataggca
     9301 atgcctgcga acccaataaa gaacattgaa tggtgaaaga gcctttacgc tgaccctttc
     9361 tgctgaaaat acctataact gcccagtaga aaacctccga agccaataac attacattat
     9421 agaaattttg tcctgccaaa acaagagaat cccctatttt ttcaaaaaaa cccatctttc
     9481 tttccggagc aggaacctta attttcaggg aggaaaaact atctataacc tcttgtattt
     9541 caggagttgc gccttgaaat aacaatatcc cttctgcgga taaacgggta tttaattcat
     9601 ccagaaaagc cactcctgca ctatctaaaa cattaacatt acttaaatca atggtattgg
     9661 gactttgatg ttttagaagc gaatttactt caatgaatgt ttccggaact gtttgcgcat
     9721 ttaaacttcc ttccagatat agaatattat ttactatctt gcaggacatt atgcctctta
     9781 atagaataga gatagccgca agaatgtggt ataatcatat accgtccagg gttttttggc
     9841 ttcatcataa gaaatattga atttaacatc taaagaaatg tttctgaaca atctgaaatt
     9901 tatcctgttt tcgttttcca aactgtaaga atgatcgggt tctcccatcc ggaaaagaac
     9961 atccaaagta gaattgatgc ttacaaactt gagcagattc aaggcagaaa gaataactgt
    10021 ggattcaata cctttggaac tgctattggg ttcttcttta tacctttcaa attcataatt
    10081 tactccatct aaggaacttg ttaccgtttt atcaaataca tagctatttt tattataatc
    10141 ctgagcccaa ccgtagccaa cgcgtaaact tgttctggca ttggaactga aattaatttg
    10201 ataggtcaat ccgattcctt ctttcagtcg taaaggatag aaggcaattt tggtttttaa
    10261 ttcggactga ttcaaaagca gagatattgt atcgcccgca gaattttgca gcattatatt
    10321 tttgttttcc gaaaaaaagg tgttttcctc aaatatatgg gtattcaaat tcattcttcc
    10381 ataaaaagca aaattcttaa gaaacttgcg ctcttccagc ggatagaaca aaagaacact
    10441 tttcagggaa taatcatcag gactaaatac taaatcctga ttggaggtta aatttaagcc
    10501 aatgtcatag atactgcggg tagtaaattg aaatggctta agcaattcat gagtatcaat
    10561 gctgttatca aactgcccgg aaagagaagc actggtagta ggtttgtctt tatccacatt
    10621 attattggaa gttaaagaaa tattaccatg aattgctccc cggtgaattc gttttgaacc
    10681 caaacctata tcttcatcgg caaaaacacc tgctccaatt aaaacattac cttcagcagc
    10741 agggtctaca atcattgtca gggtcttttt ttcaccttcc gctaaagtaa cagtggcaaa
    10801 atccctcaaa tcactccagg aaccacctcc aagctttact aaataggaac ccggaggtaa
    10861 aatccaaagt ttatctacag cgccaacttc gtcttctccc atactgaaca tacccccgct
    10921 gtaaatttta tagccctcgc cgtctgtatt ttttttccag agatcatagc ccattcggac
    10981 tctggctttg gattcatcca taacaactat tttcagctct ccccagtttg gctttacaac
    11041 tgttctctga cggggtaaaa ttttaacggg aataattaaa tcacccattt caccaattat
    11101 tactctgtaa ttaccaggtg ttagtaatgc cggcacaccc ataatcaatt catccgtaga
    11161 gattactgtg ttcaacgagt ccaattcttc aatagtgtaa cgaatttgct cctctgtttg
    11221 agttttagta ataaccatca gctctccaaa gtcaaaatct gtttccctag ctatcatttc
    11281 ttcataaaca tacctggcaa tcggagaagt tgaagcagcc aaagatttac tttcaacagg
    11341 caagccactg aaatataaat tgcattttgc agcaaagagg tctgtctctt ccataatcat
    11401 ttcattaaat gcctgcacca gatagatcat agaagggtct ataagttcca tagtgcgttc
    11461 acccttatga ctaaaaacaa ttctttcgga agtggaatcc cgcaacacaa attccatatt
    11521 caaataagct ctgggttctg tgcctgcaat cttttcaatg ttataaacct ttgtctccag
    11581 aaaatagtgg ggattttttt ccagctgttc ccctctggaa actgttccaa aaatattata
    11641 cgcttttaag cgctggacaa ttaaattggg aatagcatca cgtaaacggg ttgcccaaac
    11701 atccgtctgc agaaatttca cattatagaa gttttctttc actacaattt gacttctatc
    11761 ataagttcta ttcagaacag tatccagcac ttccagattt tttcctgtat ttgtagtacg
    11821 gcgcaattcc ggtttttctg ttcccggttg atattccaaa acataatagt ttacaggagt
    11881 tctttccact ttttgcaaac agccggtaag caaaagtaga atagccagaa atcctgaaaa
    11941 gatgtataga cattttcttt taagcattat agctcctaat ttccgcgtag taaaatagca
    12001 ggtgtatctg caatctgacg agaaaattca ttcaggtttt cactcgtctc ggttaaagta
    12061 cttaaaattt ccagcaggtc accttgtccg cgcgttacaa ctctattaag attagaaacc
    12121 agagttccgg ttttttggat agttgtggaa agttcagtga ccatactttt taaatctgtt
    12181 cgggaaagtt cgccactaag ttttccaagg ttggtcatta agctgtcaaa acctggagta
    12241 taaagaatgg cattaacctg atccaatgct ctattgatat tattgctgat agttaatatt
    12301 tcgcgtgttg tctctaagat catttgatca ctatgttcac caactgtaga gaccttcaaa
    12361 tttgcctcgc gaattaaagc agaaccatca tttgtcaatt gagttaagct tgtttccagt
    12421 tttgcagtta gttcctccag ttgttgattt acattattgg taacactaat caaacttgcc
    12481 tgtgaggtct cggaaacatt ttgaatattg cttgttgcac tgctggtgat ggcatcaaga
    12541 tttttggtaa gattttctgt aaccgcgctt aggtctttac ttagttcttc tgttaaatcc
    12601 gcagtattat tagctatgcg actaatactt tgcatagttg tagccaggtt ttcatgggtg
    12661 tcttttaaaa ttaaacttgt ttgagcaagt atggtagaaa tattgtttct gttttcctca
    12721 ccggttatag cattcaagtt ggcagcaatc atatcaattt tatctacaat ggattttgcc
    12781 ctttcactaa tatcttccaa taccgttgtt ccggttttaa tgtaactttt gggttttagt
    12841 aactttgctt cattagttcc accccttagc tccacatttt tcagtccggt aattccggat
    12901 aacaaaagaa ccgcttcggt atcctctttt ataggagttc ctctgttcac gctgatagta
    12961 ataattattt tggttacatc cttggggtta attttaacat cttccaccct gccaattttt
    13021 attccgcgat aattaaccga actgccaact tgcaaaccgc ttacagaata gtcctcaaat
    13081 tcaatataat agatgtcttt cttttccact aatctgctac ctgccacaac tatggcaaaa
    13141 agtattatca ggatagctcc aatgattaaa aaaataccca gacggatttt agcggctttg
    13201 gaaaccattt tatcctcctg tccggtcttt agatttgtta attgcagaat ccaaattcac
    13261 ctctccacta agtaaacagt tttgaacaaa cacgggagaa ctggctttca gggttacttt
    13321 tccttcacag ataacatcct ctccgaagct tacatcgcca tcaattttca gttctttgca
    13381 ttcacttaaa gaaggaatac ctttgctgaa tcttttttgc aaatcttcta ttttaccgta
    13441 atatttctca tccagttcaa ttacaggtgc cttttctacg cctcttttca aaacaatctg
    13501 atattgttca ttcagttcat aagcatcaga ccagagagct aaaagttcat ttgttttttt
    13561 caccggcaca aaacgctccc gacttactac taatgcttta gaattattga acacactaat
    13621 ggctgaaccc attgctgttt ccaattgata aaccggagtt ccatccacaa ctttaggatt
    13681 aactattaac ggtaacaaca ttaaaccctc attggcaatc atttgccatt ccagggctcg
    13741 gaggtcaatc cagatattgt tggtgttgaa atatttataa tagtcaatat cctgaaagcg
    13801 aggtaaatcc tcttcaggac attgagcaat ttctcttaaa agcaattgtc ccgatttatc
    13861 ttcacaaagg tgaccgcctt ttttatccat aggactgcgt aaacagacct ccattacgaa
    13921 aggaatattg ttattttcca tataagcagg aattgatgta tctacaacag ctcccaggtt
    13981 atcagcatta gaaacaaaag cataccggta gtccttggca atcattttat ccagtaaatc
    14041 ccctaaagct gtataaatat cgccatgccc cggaggattc cacattaatt ttgtctcttt
    14101 attttcgtaa ggcataagag tatcctgacg aatacgggga aatttattct gcaaaaaaga
    14161 aagaggcaaa tcctgtttac ctaaatcagg atatttttcc aggtatttta aagtatcggc
    14221 ttcggtattg aaactattca taaacaacag gagaacatca tatccggaaa gtgaccttaa
    14281 agtaagaacc tgccgactga taatatccag aaagttcaga ttatttttca caggcaaaag
    14341 tgatttcgct ttagaaagcc ccatacttgt tcccaagcca ccattaagct taattacaac
    14401 tgtatttttc aatattgaag ttcgggacag atgttttatt tgttgataat caattagatt
    14461 attttccgaa ggcggtctta ttgcatcttc accaataagc cctttttcac ccttaacaag
    14521 tttctgataa tatgcctcaa aagtccggat aacctcatcc cttatgcctt cctgtttcat
    14581 caaccggata aagggagtgc tcatatttat gctccaaatt ccttatttac aagatatttt
    14641 accatttctt cgtccacctt ttccgccagc gttatattgc cttcagcact gtgtttttcg
    14701 ttccaggcag ttccagtttt aattgcacct tcattttgag cccaagctcg gcgcgcaatt
    14761 cctcccataa catcccagga aagaccactt tttacaactt catccattct ttcgctgcca
    14821 tccaaaacaa ttccatttcc accattgata caacggctaa ctccaactcc acctccattg
    14881 gaaagaacta ccatagtcat tcctctggca atatttccgg caaagcaatg ggtagccatt
    14941 tctgccatta aatttgagcc gtcataaata ttggctgttt cgcggaaagg agaatccgtc
    15001 cctgaaacat cgtgatggtc tcttcccaac ataacaggac caatttcacc cttacgaacc
    15061 atctcgttaa atttcagccc aagtttaatt ctgctttctt catcggcata aagaattctg
    15121 gctttagttc ccaccaccag tttattctct tccgcagtgg aaatccaata gtaattatct
    15181 ctatccatag cactacgatt ggggtctatc aaagaaatag ctgccaggtc tgttttatgc
    15241 aggtcttcat cttttcggga aagacaaatc cagcgaaaag gtccgtaacc ataatcaaaa
    15301 cagataggac ccataatatc ttctacataa gatggccaaa taaaaccctc gttggtattt
    15361 attccgttgc gggcaatttc tgttatcccc gattcaaata cacttgccat aaagctgttg
    15421 ccataatccc aaaatttaga gcccttagca gttaaatttt tcagcacatt aaaatgcctg
    15481 cgcagtgaag catccacttt ttgcttgaat aattcaatat tttcagccaa aagtttacgc
    15541 ccttcttcat aagtaattcc tgcaggagtg taaccacctt catatacagc atgacaggat
    15601 gtctgatcgg aaagcaattc cacttttatg ttatgctgat ctatgtattc cagtaaatca
    15661 actatgttgc cataatatgc aatggaaagc ggtttttggg tatgtctggc ttcgtctacc
    15721 cagttaaaaa tctctgccaa attactggag accttactaa cccaaccctg attatatctg
    15781 gtttctattc tgcttttatc cacttcggca ataattccaa tgccaccagc aatttctatt
    15841 gccttacttt gcgcaccgct cattcctccc aaacctgatg aaacatataa aacccccgcc
    15901 aagtttttgt cttccggaat tcctaaatat tttctgcccg cattcagcaa agttatataa
    15961 gttccattca caattccctg aggacctata tacatccatc cgccggcagt catttgaccg
    16021 taatttgcca ctcctaaagc tgtcaggcgt ttaaattcaa tcgggttatc ccattttcca
    16081 accattaaac catttgtaga tataactctt ggtgcttccg gacgcgaagg aaaaagccct
    16141 aaaggatgtc ccgaagccac aaccaatgtc tgattttccg tcataatttc caggtattgt
    16201 ttaattaatc tatattgcat ccaattctga cacacctgcc ctgattctcc ataagttacc
    16261 agttcataag gatagagcgc cacatcaaaa tccaaattgt tatctatcat cacttggata
    16321 gctctggcag gagtaatgcc tttatattca tctatcggtt tgccgtaaat atgaccttga
    16381 ggacgatagc gataaccgta aattctacct ctggttttca gttcctgcaa aaattccggt
    16441 gccagaacac tatgtaattc tttgggaata tagcgcagag cattctttaa agccagggct
    16501 atatcttttt tgcttaaatc caatcccctg tccggagctc tccgaatatt tggttcaaaa
    16561 gttggctccg gaggaagttc cgcaggtaaa tgaacttcat attttctatt aggcattatt
    16621 ttttatctcc tttattttat cttgtttaac ataatattta gaatagtgct gtcggcatct
    16681 tgcattccct tatttacaaa ataagccaaa ttgtcaatag tgccttctat gctatccgta
    16741 attatgccat cattggaaga aacacaaatt cctgttttcg ctaaaagagc agcttgaacg
    16801 gcagcacttg tagtggtggc aacttttaag gaacaacctt ctttagcacc gtcacaaacc
    16861 attcctgctg tatcagctat catattttta atggcatatt caatttgagg ataggttcct
    16921 tttaaaagca taactatgcc acttgcagct ccagcagaag cagaaaggca tccgcaaata
    16981 caggaaagaa tgccgatttt ttgttttata tgcacactca ccaaatgccc catagctaaa
    17041 gctctgatta gttcttcttg gttactgcca agtttttcgg caactgcaat tataggcaga
    17101 gtaatagcca acccctgatt tccgcttccg gaattactaa tcacaggcaa attgcatccg
    17161 ctcattctgg catcagttgc agccgcagtt aaagccattg cataatgatg aatgtcatca
    17221 cccaaaagac cgctatttat ttgctccata atcatcttgc ctacattaat accggatttg
    17281 ttggataaac caaaatctgc tattctacgg tttaaaatca atcccaaatc aattaattcc
    17341 aatttatcaa tatccgtatc cctgcaaaaa tcgtaataat ccttcaaagt aaaagcagat
    17401 ggtgatatta aacggggtgc ttcttcagcg cctatttcgt tgtgttgcaa aatatgctta
    17461 tctttttccc aatgatacaa ccaattatga cgccagcgta aaattgcctt gctttcagat
    17521 tttgccgtct ttaaagaagc ttcaatccat atcttttctt ccgtttcttt cattttggtg
    17581 tttattcttt tttctgccag catttgtttg cccgcgttga ttgtttccgc atttaaatct
    17641 aataaaacct ccagaccctt ttccggctcg gcaactaata ttcctaaagc aaaagcaatt
    17701 tccaaaccca ttaaatttgt attgggaatc ccaactccca ttccgttttt taaaatattg
    17761 gggcttacaa tcaaatcggc agatataatt gtatccttta aaacacttga agcataagca
    17821 cctgctaaag caacagctgc cggttccgta caccctaaag caggagaaac ctcttgtttt
    17881 aagagtgcca agatattttc cgtatccttc atcacctatc ctaatattca tttttgcttt
    17941 tatactattc tttacatcgg ttttattccg tcaagcaaaa aaggaaaaag gattttttac
    18001 actgaaagca ccgaaaaaaa agataagcac gcagattgcg ctgataacgc agattgaata
    18061 ggtctgagat gaacaggatt attttagcag agggctgagg gcttagagcg gagagaaaaa
    18121 ataggtctgt gataaacagg attatattgt tttcagagat tttttaattg aagtgtaatt
    18181 gtaatagaat ttctcatttg aaaagtcata ttagagatgc gtagtcggaa ctcgccgaag
    18241 ctccgcattt ttattatatt tttaatttca cttttttgca gaggcacagt gtcctccgac
    18301 taaacc
//
LOCUS       DAOPFL010000030        17758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_3545_length_17758_cov_105.226798, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000030 DAOPFL010000000
VERSION     DAOPFL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17758
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3545_length_17758_cov_105.226798"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="PLG69_03630"
     CDS             <1..1265
                     /locus_tag="PLG69_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPI42632.1"
                     /translation="KIVQYIQKHCIADDWTLKITKDDSHETRFAQNVITQHIAGAMKE
                     ISLSVSFGSKSGSCTVNQDDEESLAYLVKTAEEIAKLAPEDPEFVPSVGKMDIPEIAN
                     CDPQTKALEPKQLVDIVQTSINKAKTFGATVSGLTEKHIETNALFTKNGFAGEYEKSD
                     FGHSMTLKKEEVETKVAYEAKNFADFNLQILLEKLLNQASSLAVKRTFEPQKIAVLLR
                     PLALQELFWFMGWMMDRRYADEGITPFTNQIGNPFFGEKFSWFSTCKQPTLLAPPFAH
                     DGIIAEEIPWVEKGILKNLATSRYWAKKIGSKPCDIYNMFIPGEGYSEEEMLQMVPRG
                     LIVNSFWYIRTVDTKAGEFTGTTRDGVWYFEDGKIQYAVNNLRFNEIPHNATGRIIAT
                     GISELANPISLLPPMLIDNFNFVDKTSF"
     gene            1408..1581
                     /locus_tag="PLG69_03635"
     CDS             1408..1581
                     /locus_tag="PLG69_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPI42633.1"
                     /translation="MPDRTLICQDCSREFVFTEGEQEFYKEKGLQNEPKRCPECRKAK
                     KAQFNRNRRRQSN"
     gene            complement(1730..1852)
                     /locus_tag="PLG69_03640"
     CDS             complement(1730..1852)
                     /locus_tag="PLG69_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42634.1"
                     /translation="MNGLIGEKVKDERVERWKSEKVKQGDNLIVNPEMYSWSSR"
     gene            complement(1880..4879)
                     /locus_tag="PLG69_03645"
     CDS             complement(1880..4879)
                     /locus_tag="PLG69_03645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPI42635.1"
                     /translation="MKHFTVFLALLVAFGLFAADLKENEGTAIFKLKPEFRSALQKSD
                     NRTGIASIDEKLQKLQVSSLSPKFSISPQKREECELSLIFTVQSPYPPQAVVNLLSSD
                     PFVQYAEIVYPEVIFAVPNDEHYSESFYFASLEAESAWDIHKGENGTQPVIIALVDTG
                     CRWTHPDLAENIWQNLGEDSNHNGYTIYYNGSTWVMDTGDLNGIDDDGNGKIDDLIGW
                     DFMATSSGGETNNPYENSGHGTNVSGILSARTNNTIGVSSLAWNLTLMPVSCSYPGSS
                     SIYNGYQGIIYAAENGADIINCSWGGTTYSQANQEAINYAYSLGSIIVAAAGNSNNTV
                     PVYPSAYQNVLAVAALQNNGVKSSVSCYGAFVDVGAPTGSIGTLSGSGYTTVSNATSY
                     ASPVGSSLAGLIKSYYPEISQSALLNRIKGSCDDVDAVNSGKENLLGEGKLNARRALQ
                     EENPLPDEEIRLALIENRGATDANGNLAVEPGETFSVNLLLRSYGEYSANGTFTLSTT
                     NPNVNILSGSYNQSIPADAYFTLEEVFNIYVLPTATSQYVTFNLQTTADLPVVAGNNL
                     SFSILINAGGIFVWEGISGGRDMSGTFIKNTLQNLGYNCVYGTTFPASFYSFDAVFLS
                     FGAVGSNIVRFDKPYMFNALYNYLISGGRVYIEGVDVVGFDLTYYLPDIQGELDAYEV
                     LWPLLGIISAEDGVTNAIDNLSGVSYTPSSGMLFTSSSQTKVDYIDRFTELYPYARSA
                     FEESDYGCVAVACAGGYNQRSFVFSYALSELTDGTFPNTRANLVTRIMDFFTAEEVTL
                     PVELTSFYATYSNGGNIYWNTASENNVLGYNLYRNSNFSLSNAVKLNTVIIPATGCAT
                     GNNYCYYDSETALSDTLYYWLESVSYSGFTQVFGPAVLIVPFTEPETPPLPPDKIEYL
                     SAYPNPFGNSVFLELKIASPAPVMVKVYNIKGQLIKNWEYPYLETGIHHLSWDGLDNK
                     QRNTTSGIYLMVVKTQERTFTRKLLKM"
     gene            complement(4889..5242)
                     /locus_tag="PLG69_03650"
     CDS             complement(4889..5242)
                     /locus_tag="PLG69_03650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPI42636.1"
                     /translation="MACEFCNLDCSVYLAENEHFFAIWDKYPVSKGHTLIISKRHTPD
                     YFNLNAEEAISLHDITLKVKKVLEEKFEPRGYNLAMNCGSVAGQSIPHFHLHIIPRFG
                     KGDKGLFPRRRESVF"
     gene            5862..7322
                     /locus_tag="PLG69_03655"
     CDS             5862..7322
                     /locus_tag="PLG69_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPI42637.1"
                     /translation="MKYKVQIVLAICLLWTMSLISAKPDPNALVGKIGDREYNYKKFN
                     DGFKSYLQYHGKGKVLTKQDSIKLNNQYWEELIGMYVYDQAIKAGKIKVTDAELEAEI
                     KKNIPAEIKQIKDFQTNGKFDKAKYEQALKDHPDFKREVMNYSRDLYSYNKLIKTIKN
                     EVKANPDSVKNAWLKENDYASAKIIHFDYNKLTAVNATDDEVRAFYEAHKDEFKRENG
                     RSYLLARFTGNLSKAENAEQKAQENKILSTALYNRAKEVGLQQAAEEMNIPLEESPYF
                     NSQDQIIPHIGRAPDLVSFAFNNPVGSIPDIFYAPTGDILVLELKSETPEYYIDFEIK
                     KQEMIIRANRAKRMYTMENYVQNFMQSETPETYLEAAIRDSIAIIDAEKVIADNEIKP
                     LGKIPALNTAILNTPEGSFTPLIENEKHWYLALVTKRQYPDLTLWEKDKKKIIADAEA
                     KLQEDHLNQWYFEQRQKVQIIDNRKDFYELQMQLKL"
     gene            7545..8099
                     /locus_tag="PLG69_03660"
     CDS             7545..8099
                     /locus_tag="PLG69_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42638.1"
                     /translation="MKRLFILLLLLIGCFGLNAQTGLFNLSYAIPIEEADSLLALSDF
                     YTPNINDNLVRYYPENSNYVEAILVFVEPQTRRIIGWFIKYSPDNSEEDDAYVLQTLQ
                     EMHGKNNYFDEETQQLIWFLSTTRSVHIMYADDNSLTVLYYDSYFPELFHLKDKKALR
                     KDSETQNNTLPEDSKPAPSAEDTR"
     gene            8213..8503
                     /locus_tag="PLG69_03665"
     CDS             8213..8503
                     /locus_tag="PLG69_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42639.1"
                     /translation="MNIHFRDVQSGSVEARAIIEIADGVFLNEVTILNIDGEIVVEFP
                     RKSFVGKSKRTYYIDIITFEDNDKRIVWELEIKNAYREWRKANKKVLIYEEK"
     gene            8527..9741
                     /locus_tag="PLG69_03670"
     CDS             8527..9741
                     /locus_tag="PLG69_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPI42640.1"
                     /translation="MITDLQNILSTNIKGMKRSAIRELLKFLGQPGLISFSGGFPSPL
                     SFPIEELKSIIAEVMENEAAFALQYGTTEGDDLLRTMLANRYKANGIDVTKDNIIITT
                     ASQQALDLISKMFIDPGDDVIVGLPSYLGALQAFGSYGANMIGIPMDDEGEDPNLMEE
                     ALKDLASKGKKPKFIYLIPDFQNPAGITMSERRRKEMLELAHKYDVLILEDSPYRELR
                     YEGETQRTLYELDGTGQVVILGTFSKIFCPGFRIGWVVGNPDVLDKIVVGKQATDLCT
                     PPFTQRIAARYMEKGYLDPKIEDIKKMYSVKQKVMLESLEKYMPEEMKWTHPQGGLFL
                     MVTGPEDLDTNALLLECIKEANVAYVAGNSFFCNGKGYNTMRLNFSYESIETNIEGCK
                     RLGSFFKKVLQK"
     gene            9761..10060
                     /locus_tag="PLG69_03675"
     CDS             9761..10060
                     /locus_tag="PLG69_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPI42641.1"
                     /translation="MSKKVEKDRMGMIIREEDTPEKMEVEITEIKAPGDEDSPVLIYY
                     NWCKKCGICVAFCPTGCLGRKSDGSPFVQAPEKCTHCENCDRLCPDFAITGAKER"
     gene            10072..11316
                     /locus_tag="PLG69_03680"
     CDS             10072..11316
                     /locus_tag="PLG69_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPI42642.1"
                     /translation="MTEKDKSKQTKHKTTSDKASLAAKPESKLEEIQAKRERKTVLMQ
                     GNEAVAYGALDAGVNFFAGYPITPSTEIAEILAAELPKRNGVFIQMEDELASICAIIG
                     ASLAGAKALTATSGPGFSLMQEGIGFAKITETPCVVVDVQRVGPSTGMPTSPAQGDIM
                     QSKWGSHGDSPAIVLYPDSVKESYELTIRAVNLSEKYRTCVILLLDEVIAHMREAVCL
                     PDLANVRIINRIKPTVPPHWYKHYDENQKYLSPLASFGEGYRFHVTGLTHDSHGFPTN
                     KPSEAAEMMERLRKKISYNIRDLVQIESYQMEDAKIAIFAAGITSRAAKAAIASARAE
                     GVKVGLLRPLTIWPFPDDAVRKMLRNVETVIVPELNQGQLIHEVQRLTKEKSDGSLIP
                     INKVSSELITPNDILNKIKEVC"
     gene            11318..12157
                     /locus_tag="PLG69_03685"
     CDS             11318..12157
                     /locus_tag="PLG69_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPI42643.1"
                     /translation="MANIPQRIIHEYLRHDKKFPHVWCAGCSNGIVLGAIIRAVASLN
                     IDRNDIVMVSGIGCSSRMPVYVDFNTLHTLHGRSIAFATGVKMHKPHLKVIVVTGDGD
                     CAAIGGNHLIHAARRNIDLKVIMINNNIYGMTGGQCSPTTPHNAYATTAPYGNIEPDF
                     NICNLAMGAGASFVARTTAFHVLEMQSMIKDALQHPGFSLIEVVSACPVIYGRLNKKG
                     GAAQMLKDFRDNSLPLTAVEKLPPEKVEGKIIRGILRKEIRPEFAAEYAALCKRVQTE
                     GGE"
     gene            12157..12693
                     /locus_tag="PLG69_03690"
     CDS             12157..12693
                     /locus_tag="PLG69_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPI42644.1"
                     /translation="MAKTFEVRLSGSGGQGLILAGIILAKAAVLENRKVTQTQSYGPE
                     SRGGYSHADVIISDKEIYFPEATNINCLLALTQEACDKFLFDLCENGILIIDTTFVKN
                     LALAAENTYEIPFTEIAQEKLGSPISTNIMALAFLVKITGIVKESSLKKAIEETVKPA
                     FVDLNLKAMQIGFDLAKK"
     gene            12919..>13307
                     /gene="mce"
                     /locus_tag="PLG69_03695"
     CDS             12919..>13307
                     /gene="mce"
                     /locus_tag="PLG69_03695"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPI42645.1"
                     /translation="MLKHISHIGIAVKDLEAGIAFYEKLGLKLEGIEEVPSQKVKVAF
                     FPCGETRIELLCPTSEESPIAKFIEKKGEGIQHIAFAVDDIEASLKQANELGLALIDK
                     EPRPGAHHSNIAFLHPKSALGVLIEFC"
     assembly_gap    13308..13407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13761..14075
                     /gene="rplU"
                     /locus_tag="PLG69_03700"
     CDS             13761..14075
                     /gene="rplU"
                     /locus_tag="PLG69_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPI42646.1"
                     /translation="MYAIVEIKGFQYRAEKNAVLRVPYLKTAEPGQTLTFDKVLLLRE
                     EEEIKVGQPVVEGASIVAEVIENGKGEKKIIYHHKRRKGYRVKKGYRESYTDLRVTDI
                     LA"
     gene            14091..14354
                     /gene="rpmA"
                     /locus_tag="PLG69_03705"
     CDS             14091..14354
                     /gene="rpmA"
                     /locus_tag="PLG69_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPI42647.1"
                     /translation="MAHKKGVGSSRNGRNSNPKYRGVKKHASESVLAGNIIVRQKGTK
                     IHPGKNVGIGRDYTIYSLIDGKVTFTIGKEGRKYVSVQPMNEE"
     gene            14489..14914
                     /locus_tag="PLG69_03710"
     CDS             14489..14914
                     /locus_tag="PLG69_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42648.1"
                     /translation="MEAQQILLLILSIIIIGTTIIVGITLYKDQAYTANKTALVAEAK
                     NYGKRITKYYQDLASLKNDNLQSASVDTTKLIKYLGWESNFIKTEAGTFNITAVSDSS
                     VIITGYAKAKKNGKSPKVVVTVTFPEGKMDLRESDSVTK"
     gene            15000..15377
                     /gene="gcvH"
                     /locus_tag="PLG69_03715"
     CDS             15000..15377
                     /gene="gcvH"
                     /locus_tag="PLG69_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPI42649.1"
                     /translation="MEIRDDLYYTDTHEWIHLDGDIATIGISDFAQHELGDIVFVELP
                     EVGQKVAASEPCGSIEAVKAVEDLISPLSGKVEEKNIDLEDSPELINKSPYEEGWLFK
                     IRMSNPEELENLLSAAEYQKIIP"
     gene            15403..16032
                     /gene="gmk"
                     /locus_tag="PLG69_03720"
     CDS             15403..16032
                     /gene="gmk"
                     /locus_tag="PLG69_03720"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPI42650.1"
                     /translation="MITKDNHFLIILSAPSGGGKSTILNEILKVTNDIDYSVSYTTRS
                     PRGTEQNGIHYHFVTEEEFQRRIDEGDFLEYAKVFGNWYGTSKSFIKDRLAKKHHVIL
                     DIDVQGASLISATDIPYVKIFILPPSMEVLHQRLVLRATDSEEEIAKRLQIAKDEVAY
                     IPQYDYLVINDKLDIAVQDVLAIIRAEENRVCRYNHPAMDFISTGEKDD"
     gene            16025..16429
                     /locus_tag="PLG69_03725"
     CDS             16025..16429
                     /locus_tag="PLG69_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42651.1"
                     /translation="MINEKLENFLEKADMNYLFCLLAQLEAYRLLQLPTYVKQKFQDK
                     ITVMAMNHVAENEVPDYITELAEAELAEAELAEAQESPFQEEEGAEEEAEETIDDSKK
                     FIEELTGTEDYEEEEEEEEEFYNEDNEDNDES"
     gene            16433..17086
                     /locus_tag="PLG69_03730"
     CDS             16433..17086
                     /locus_tag="PLG69_03730"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPI42652.1"
                     /translation="MSVNALRQYLEFLKNSGIKELYYPPSDKQKKLTELQKQYSTCTK
                     CPLHNSRHKFVYGEGNPNAIAMLIGEGPGEQEDITGRPFVGAAGQLLEKMLSAINLKR
                     SDVYITNIVKCRPPGNRNPESEERKACLPYLLEQIQIIQPKLLLILGLVAAQTLLNNN
                     NTLGWHRGKIHYVMDIPAFVTYHPSALLRNQEWKKPAWIDLQEFQKEYEKLLAATIS"
     gene            17227..>17758
                     /locus_tag="PLG69_03735"
     CDS             17227..>17758
                     /locus_tag="PLG69_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPI42653.1"
                     /translation="MIKQRNSPLSIFKLNLPISLLITFLLTFLVVGLDARFAIYGDTR
                     SNPKIHQQIVQQIVSRKPEIVFHTGDLNSSGKTQKEYDNFLQIISPISQSARFFPARG
                     NHEKDLTLFLQNFPYLNSSSYYTVFQDGIRFIILDSVLELSPGSEQFSWLQKQLKEDI
                     PSMLILHHPVFSSGEHN"
BASE COUNT         5802 a         3343 c         3483 g         5030 t
ORIGIN      
        1 aaaaaattgt gcaatacatc cagaaacact gcatagcaga tgactggaca ctaaaaatta
       61 ctaaagatga cagccacgaa acacgctttg ctcaaaatgt tatcactcaa catattgccg
      121 gggcaatgaa ggaaatttct ttaagcgttt ctttcggtag taaatcggga agctgcactg
      181 taaatcagga tgatgaagaa agtttggcat atcttgttaa aacagctgaa gaaattgcca
      241 aattagctcc cgaagaccct gaatttgttc cttccgtagg aaaaatggac attccggaga
      301 ttgcaaattg tgatcctcaa actaaggctc tggaacctaa acaattagta gatattgttc
      361 aaaccagcat taacaaggca aagacatttg gagcaacggt ttccggttta accgaaaagc
      421 atattgaaac caatgctcta tttactaaaa atggttttgc tggtgaatat gaaaaaagtg
      481 attttggcca ctctatgaca ctgaaaaaag aagaagtaga aacaaaggtt gcctacgaag
      541 cgaaaaattt tgcggacttt aacctacaaa tccttttgga gaaactgctc aatcaggcat
      601 catctcttgc tgtaaaacgg acatttgaac cccaaaaaat agctgtcctt ttaagacctc
      661 ttgctcttca ggaattattt tggtttatgg gttggatgat ggaccgcaga tatgcggatg
      721 aaggtatcac tccttttaca aaccaaattg gaaacccctt cttcggagag aaattcagct
      781 ggttttctac ttgtaagcag ccaactcttt tagcaccacc tttcgctcac gatggcataa
      841 ttgccgaaga aattccctgg gtggaaaagg gcattttgaa aaaccttgct accagccgtt
      901 attgggcaaa aaaaatcggc agtaaacctt gcgatatcta taatatgttc attcctggtg
      961 aaggatatag tgaagaagaa atgctgcaaa tggttccccg cgggcttatt gtaaacagct
     1021 tttggtatat tcgcactgtg gatacaaaag caggtgaatt tacaggaacc actcgcgatg
     1081 gtgtatggta ttttgaagat ggaaaaattc agtatgccgt aaataatttg cgctttaatg
     1141 aaattcctca taatgcaacc ggaagaatta ttgctacagg tatcagtgag ctggcaaatc
     1201 ctatttcttt gttgcctcca atgctcattg ataacttcaa ttttgtagac aagacctctt
     1261 tttagaaaaa ataaattgac agaaatttga aaactaaaaa agggtatcta caaatgcgat
     1321 tcaacattcg ccctaagcca attccttcta ttacgggagt gcctttggac cagaatgttc
     1381 gatcccgtta ataaaaggag ataccaaatg ccagacagaa cactcatttg ccaggactgt
     1441 tccagagaat tcgttttcac tgaaggtgaa caggaattct ataaggaaaa aggccttcag
     1501 aacgagccca aacgctgccc cgaatgtcgt aaggcaaaaa aggcacagtt taatcgtaac
     1561 agacgcagac aatctaatta gatagtttct aatctgttaa cgccactgct aaaatgtagt
     1621 ggcgtttttg tttttaatgg cgagatgttt tagaaaattg ggaatttttg ttgtgtagct
     1681 ggaggacgcc gtttctccgc aatttaaatt ttcctaataa aaagcggagc taccgcgagc
     1741 tccagctata catttcaggg tttacaatca aattatctcc ttgtttcact ttttcacttt
     1801 tccacctttc caccctttca tctttcactt tttcacctat taacccgttc acctgttaac
     1861 cccttcaccc gttcaccact cacattttta acaatttacg ggtaaatgtt cgttcctgtg
     1921 ttttaaccac catcaaatat attccggaag tagtgttgcg ctgtttatta tccaagccat
     1981 cccaggaaag gtgatgaatt cctgtttcca gataaggata ttcccagttc ttaatcaatt
     2041 gtcctttaat attatagacc ttcaccataa caggagcggg agaagcaatt ttcaattcca
     2101 aaaaaacaga attgccaaaa ggattgggat aagcgcttaa atattcaatt ttatcaggtg
     2161 gcagaggagg tgtttcgggt tcagtaaaag gcactatcag aacagcagga ccaaaaacct
     2221 gagtaaaacc actgtaagaa accgattcca accaataata caaagtatcg gacaaagcag
     2281 tttcagaatc gtaatagcaa taattatttc ccgttgcaca acctgttgcc gggataatta
     2341 cagtattcag tttaaccgca ttggacaaag aaaaattgct attccggtaa agattgtagc
     2401 ccagcacatt gttttcggaa gcggtattcc agtagatatt acccccatta gagtaagtag
     2461 cataaaaaga tgtaagctca accggcagag taacttcctc tgctgtgaaa aaatccatta
     2521 tgcgagttac aaggtttgct ctggtatttg gaaaagtgcc atccgtcaat tcgcttaaag
     2581 catagctgaa gacaaaactc cgttgattat aaccaccagc acaagctacg gctacacatc
     2641 cgtaatcact ttcctcaaag gcacttctgg cataaggata gagctctgta aagcggtcta
     2701 tatagtccac tttggtttgc gaagaagagg taaacagcat tccggaggaa ggtgtataag
     2761 aaacaccgct aaggttatct atagcgttag ttacgccatc ttcggcactg attattccca
     2821 aaaggggcca gagaacttca taagcatcaa gttcaccttg aatatctgga agataataag
     2881 ttaaatcaaa accgacaaca tccactcctt caatatagac cctgccacca gaaattaaat
     2941 aattataaag ggcattaaac atatagggtt tatcaaaacg cacgatatta ctgcctaccg
     3001 cgccaaaact taaaaaaaca gcatcaaagc tataaaatga agcaggaaaa gtagttccgt
     3061 aaacacaatt gtagcccaaa ttctgcaagg tgtttttaat aaaggttccg ctcatatctc
     3121 ttccgccgga aatgccttcc caaacaaata ttcctccagc attaataaga atgctgaaag
     3181 ataggttatt tcctgctaca accggtaaat ccgcagttgt ctgcaaattg aaggttacat
     3241 attgagaagt tgcagttggc agaacataaa tattaaagac ctcttccaaa gtaaaataag
     3301 catcggcagg aatgctttga ttgtagctac cggaaaggat atttacatta ggattggtgg
     3361 tagaaagagt aaaagtgcca ttagcagagt attcaccgta acttctcagc aacagattta
     3421 ccgaaaatgt ttcccctggc tccactgcca aatttccatt agcgtctgta gcacctctat
     3481 tttctattaa ggcaaggcgg atttcctcat cgggaagagg attttcttct tgcagagcac
     3541 gccttgcatt caatttgcct tcacccaaaa gattttcttt tccggaattt actgcatcaa
     3601 catcatcgca acttcctttg attctattga gaagagcact ttggctgatt tcgggataat
     3661 atgatttgat taagcccgct aaagagctcc ctacgggact ggcataagaa gtagcattac
     3721 taactgtcgt ataaccactt ccggaaagag tgccaatgga acccgtagga gcaccgacat
     3781 ccacaaatgc accgtaacaa gaaacggaag atttcactcc gttattttgc agagcagcta
     3841 ctgccaaaac attttgatag gcggatggat agacaggaac tgtgttatta ctattacctg
     3901 cagctgcaac aataatagac cctaaagaat aagcataatt tatagcttcc tgatttgcct
     3961 gcgaataagt agttccaccc caactgcaat ttatgatgtc cgctccgttt tcagccgcat
     4021 aaataatacc ctgatagcca ttatatattg aacttgagcc aggataactg caggaaacgg
     4081 gcattaaagt taaattccag gcaagggaac tgaccccgat tgtattattt gttctggcag
     4141 aaagaattcc ggaaacattt gtgccatggc cactgttttc ataaggatta ttggtttccc
     4201 ctccactact tgttgccata aaatcccaac caatgaggtc atcaattttt ccatttccat
     4261 catcatcaat tccattcaag tcacccgtat ccataaccca agtgcttccg ttataataaa
     4321 ttgtgtaacc attgtggttg ctatcttccc ctaaattttg ccaaatattt tctgccaaat
     4381 caggatgagt ccatctgcag ccggtatcta ccagagcaat aattacaggt tgagtgccat
     4441 tttcaccttt atgaatatcc caggcggatt ctgcctccag agaagcaaaa tagaaagatt
     4501 ctgaataatg ctcgtcattg ggaacagcaa aaatgacctc gggataaaca atttccgcat
     4561 attgcacaaa ggggtcagaa gaaagtaaat ttacaactgc ctgaggtgga taaggacttt
     4621 gcactgtaaa aattagagaa agttcacatt cctctctttt ttgcggcgaa atgctgaatt
     4681 tagggcttaa ggaactgacc tgcaatttct gcagtttttc atctatagac gcaattcctg
     4741 tgcgattatc ggacttttgc aaagcagaac gaaattccgg tttcagctta aaaatagccg
     4801 ttccttcatt ttcctttaaa tcagcggcaa aaagtccaaa agcaaccaat aaagctaaaa
     4861 aaacggtaaa atgtttcatt tccgcctcct aaaaaacgga ttctcgcctt cttggaaaga
     4921 gacctttatc tccttttccg aaacgaggaa tgatatgcag atgaaaatgg ggaatacttt
     4981 gtcccgcaac agaaccgcaa ttcatagcca aattgtaacc acgcggctca aatttttcct
     5041 ctaaaacttt tttcaccttc agagttatat cgtgcaaaga aatcgcttct tccgcattta
     5101 gattaaaata atcgggtgta tgacgcttgg aaataatcag ggtatgaccc ttgctaacag
     5161 gatatttatc ccaaattgca aaaaaatgtt cattttctgc cagataaact gagcaatcaa
     5221 gattacaaaa ttcacaagcc ataagttgtt acctcttttt tatattataa ttcattcttt
     5281 cagctaagcc aaaataatgt ttggcaggga ataaaaacaa gctcgttagg tataatgttt
     5341 cagtatcgct tattctttgc taaaatctgc tgtaatatag atgtaaataa ataaggtaag
     5401 aaatctcttt atccttttaa ctcactataa tattatatag ttagctgtat attttgcctt
     5461 tcttgtttga agatgtgtat ttgcttggta tatagacaat taagtaacga ctccgtaaca
     5521 cttccgtaac acttccgtag tttcgttacg gagttgttac ttgagtgtta cttggttatt
     5581 acaagatcaa actgaaaggt taacctccct ttcaaacaca ataaaaatca ggagccattt
     5641 gcgtcttaac atttttatta tttccgatat ctttacaagc gcaatataac ttgcgacaga
     5701 ttgtgaagcc ggagttgcag tgagctccgg ctacacaaat taaaaatggc aggatatcgt
     5761 tttcaaggac gccggtgcaa tttttttctt gcagtaaaag cattgtttca aaagtattgt
     5821 tttatagagg caattgcgtc tcttattttg aggagtaaga aatgaaatat aaagtgcaaa
     5881 tcgttttggc aatatgttta ttgtggacaa tgtccctaat ctctgccaag cccgatccca
     5941 atgctttagt cggtaaaatt ggagatcggg aatacaatta caaaaaattt aatgatggat
     6001 ttaaatcata tctccaatat cacggaaaag gaaaagtcct caccaagcag gatagcatta
     6061 aattgaataa tcaatactgg gaagagctta tcgggatgta tgtttatgac caggcaataa
     6121 aagctggtaa aataaaagtt accgatgccg aactggaagc agaaatcaag aaaaatattc
     6181 ccgcagaaat taaacagatt aaggattttc aaaccaatgg caaatttgat aaagccaaat
     6241 atgaacaggc attaaaagac catcccgatt ttaaacggga agttatgaat tattcccgcg
     6301 acctctattc ctataacaag ttgattaaaa ccattaaaaa tgaggtaaaa gccaatccgg
     6361 atagcgttaa aaatgcctgg ctgaaagaaa atgattatgc cagtgccaaa attattcatt
     6421 ttgattacaa taaattgact gccgttaatg ctactgatga tgaagtccgg gcattctatg
     6481 aagcccataa agatgaattt aaaagagaaa atggacgcag ctatcttttg gctcgtttta
     6541 caggcaattt gagtaaagca gaaaatgcgg aacaaaaggc acaggaaaat aaaattttaa
     6601 gcacagcact ttataaccgg gcaaaagaag taggtttaca acaggcagct gaagaaatga
     6661 acattcctct ggaagaaagt ccctatttta attcccagga tcaaattatt cctcatatag
     6721 gtcgtgctcc cgatttggtt tcttttgcct tcaataatcc cgttggttcc attccggata
     6781 ttttttatgc tcccacaggt gatattctgg ttttggaatt gaaaagtgaa acccctgaat
     6841 attacattga ttttgagatt aagaaacagg aaatgattat ccgggcaaat cgcgccaaac
     6901 gaatgtacac aatggaaaat tatgtgcaga attttatgca aagtgaaaca ccggaaacat
     6961 atttggaagc agcaattaga gatagcattg ctattattga cgcggaaaaa gtgattgccg
     7021 ataacgaaat taaaccctta ggcaaaattc ccgctttgaa tactgcaatt ttaaatactc
     7081 ccgagggctc ttttactcct cttatagaaa atgaaaaaca ctggtatctt gccctcgtta
     7141 ccaaacgcca atatcccgac ttaactctct gggaaaaaga taaaaagaaa atcattgccg
     7201 atgcggaagc aaaattacag gaagatcatc tcaatcaatg gtattttgaa caacgccaga
     7261 aagttcagat aattgataac cggaaagatt tttatgaatt gcaaatgcag ctgaaattgt
     7321 aggaggtctt gaggttttga ggtcttgagg tttaacacac tctaaacaga gaaaatggta
     7381 atataattgt aaatctttca tcctgaaaat cctcaaaatt ctgttcatcc cagacctatt
     7441 ctttcatctg cgaaatctgt gaactacttc attttttcag attttaattg acgaaccttc
     7501 tatgccttta aaaatggaaa aaatattata taatggacta cctaatgaag agattattta
     7561 tcctcttact tctgctgata ggttgttttg gacttaatgc ccaaaccgga ctttttaatt
     7621 taagctatgc cattccaatt gaagaagccg attccctgct tgctttaagc gacttttaca
     7681 ccccaaacat aaacgacaac ttagtgcgct attatcctga aaatagtaat tatgtagagg
     7741 caattctggt ttttgtagag ccccaaacaa ggcgtattat tggctggttc ataaaataca
     7801 gtcctgataa ttctgaagaa gatgatgctt atgtgcttca gaccttacag gaaatgcatg
     7861 gcaaaaacaa ttattttgat gaagaaacac aacaacttat ctggttttta tccactactc
     7921 gcagtgttca tattatgtat gccgatgata acagtttaac agttctctat tatgattctt
     7981 acttccctga acttttccac ctcaaagata aaaaagcttt acggaaagat tctgaaaccc
     8041 aaaataatac tttacccgag gactctaaac ctgctccttc tgctgaagac acgagataat
     8101 tagttttatc taaaaatatt catactttta aaagtatact atgtagcaat aaataacttg
     8161 accaattaaa gcatttattt tttaaagaaa aagataagaa tttggagtga ttatgaacat
     8221 acatttcaga gatgtccaaa gtggtagtgt tgaagccaga gcaattatag aaattgcgga
     8281 tggcgttttt ttaaatgaag ttaccatttt gaatattgat ggtgaaatag tagtggaatt
     8341 tcctcgtaag agttttgtag gtaaatccaa acgcacctat tatatagata ttattacttt
     8401 tgaagataat gataaacgga ttgtgtggga actggaaatt aaaaatgctt accgggaatg
     8461 gcgaaaagcc aacaagaaag tgctaattta cgaagaaaaa taaaaaaaga aaaggaggat
     8521 cataaaatga tcaccgattt gcaaaacatt ctctccacca acattaaagg tatgaaacgt
     8581 tctgctatcc gtgaactttt aaagtttctc ggacaacccg gtttgatttc attttccggt
     8641 ggttttccca gccctctttc tttcccgatt gaagagctga aaagcatcat tgccgaagtt
     8701 atggaaaacg aagctgcttt tgcacttcaa tatggaacaa ctgaaggtga tgatttatta
     8761 agaactatgc tggctaatcg ttataaagcc aatggcatag atgttaccaa agataatatc
     8821 attattacaa ctgcctctca acaagcatta gaccttattt ccaaaatgtt tattgaccca
     8881 ggcgatgatg ttatcgttgg cttaccttcc tacttaggag cacttcaggc atttggttct
     8941 tatggagcta atatgattgg tattcctatg gatgatgaag gtgaagaccc aaacctgatg
     9001 gaagaagcat taaaagacct tgcttctaaa ggtaaaaaac ctaaatttat ctatctgatt
     9061 cccgatttcc aaaatcctgc tggaattaca atgagtgaac gccgtcggaa agaaatgctg
     9121 gaactggctc ataaatatga tgttctgatc ctggaagata gtccttaccg ggaacttcgc
     9181 tatgaaggtg aaactcaaag aactctctat gaacttgatg gaacaggtca ggttgttatt
     9241 ttaggcactt tcagcaaaat cttctgccct ggttttagaa ttggctgggt tgtaggcaat
     9301 cccgatgttt tagataaaat agttgtggga aaacaggcaa ccgatttatg tactccgcct
     9361 ttcactcaac gaatagctgc ccgctatatg gaaaagggtt atcttgaccc caaaattgaa
     9421 gatattaaaa agatgtattc cgtgaaacaa aaagtgatgc tggaatccct ggaaaaatat
     9481 atgcccgaag aaatgaaatg gactcatcct caaggcggtt tgttcttgat ggttacagga
     9541 cctgaagatt tggatactaa tgctcttctg ctggagtgta ttaaagaagc aaatgtagct
     9601 tatgttgccg gaaacagttt cttctgcaat ggcaagggct ataacacaat gcgtttgaat
     9661 ttcagctacg aaagcattga aaccaatatt gagggatgca aacgcttagg tagtttcttt
     9721 aaaaaggtat tgcaaaaata aataagtatt ggaggaaaga atgtccaaaa aagtcgaaaa
     9781 agaccgtatg ggcatgatta tacgcgaaga agatactccc gaaaaaatgg aagtagaaat
     9841 aacggaaata aaagctcctg gcgatgaaga ttcacctgtc ctcatctatt ataactggtg
     9901 caaaaaatgc ggaatctgtg tggctttttg tccaaccggt tgcctgggca gaaaaagtga
     9961 cggctcgcct tttgtgcagg caccggaaaa atgtactcat tgcgagaatt gtgatcggct
    10021 ttgtcctgat tttgccataa ccggtgccaa ggaacgctaa aggagtttat gatgacagaa
    10081 aaagataaaa gtaaacagac caaacataaa accacctccg ataaagcatc actggctgca
    10141 aaaccggaaa gcaaactgga agaaattcag gcaaaacgcg aacgcaaaac agttttaatg
    10201 caaggcaatg aagctgttgc ttacggtgct ttggatgctg gagtgaattt ttttgccggc
    10261 tatcctatta ctccttccac ggaaatagcg gaaattttag ccgctgaact tcccaaaagg
    10321 aacggtgtat ttattcaaat ggaagatgag cttgcctcta tttgtgctat aataggtgct
    10381 tctttggcag gagctaaagc cctcactgct acaagcggac cgggtttttc tttgatgcag
    10441 gaaggaatcg gttttgccaa aattacggaa acaccttgcg tagttgttga tgttcaaaga
    10501 gtaggaccca gcacgggaat gccaacaagt cctgctcagg gtgatattat gcaatccaaa
    10561 tggggttcgc atggtgattc tcccgctata gttctttacc cggattccgt taaagaaagc
    10621 tatgaactaa ctatccgggc tgtgaattta agcgaaaaat accgcacttg cgtaattttg
    10681 ctgttggatg aagtaattgc tcatatgcgg gaagcagttt gccttcctga tcttgccaat
    10741 gtgagaatta tcaatagaat taaaccgact gtgcctcctc attggtataa gcactatgat
    10801 gaaaatcaaa aatacctttc tccccttgcc agttttggag agggctaccg ttttcatgtt
    10861 accggtttaa ctcacgattc tcatggcttt ccgacaaaca aaccaagtga agcagcagaa
    10921 atgatggaac gtttacggaa aaaaatatcc tataacattc gtgacctcgt tcagattgaa
    10981 agctaccaga tggaagatgc caaaattgct atttttgcag ccggaatcac ttctcgcgct
    11041 gctaaagcgg caattgcctc tgcccgagcc gaaggtgtta aagttggact tttaagacct
    11101 ttaactatct ggcccttccc tgatgacgca gttcgtaaaa tgcttcgtaa tgtagagaca
    11161 gttattgttc cggaacttaa tcagggtcag ctgattcacg aagtgcaacg gttgactaaa
    11221 gaaaaaagtg acggcagttt gattcctatt aacaaagtaa gcagtgaatt gattactccg
    11281 aatgatatcc taaacaaaat caaggaggta tgctaaaatg gctaatattc ctcaacgcat
    11341 tattcatgaa tacctgcgtc acgataagaa atttccccat gtctggtgtg ccggatgcag
    11401 taacggtatt gttttaggtg ccattatccg tgccgttgcc tctttgaata tagaccgcaa
    11461 tgatattgta atggtttcag gcattggctg ctcttcccgg atgcctgttt atgtggattt
    11521 taatacactt cataccttac atggacgcag tatcgctttt gctacaggcg taaaaatgca
    11581 caaaccgcat ttgaaagtaa tcgttgtaac cggtgatggc gattgtgccg ctattggagg
    11641 taatcattta atccacgcgg cgcgcagaaa tatagacctg aaagtgatta tgatcaataa
    11701 taacatttat ggaatgacag gtggacaatg cagtcctact actcctcata atgcttatgc
    11761 tactactgct ccttatggaa atatagaacc cgattttaat atctgcaatc tggctatggg
    11821 tgcaggtgct tcttttgttg ccagaacaac agctttccat gttctggaaa tgcaatcaat
    11881 gataaaagac gctcttcagc atccaggttt ttctttaatt gaagtggtaa gtgcctgtcc
    11941 tgtaatttac ggacgcttga ataaaaaagg cggtgcagct caaatgctga aggacttccg
    12001 cgataattcc cttcctctta ctgccgtgga aaaactgcct ccggaaaaag tggaaggaaa
    12061 aatcattcgc ggtattttac gcaaagaaat ccgtcccgaa ttcgctgccg aatatgcagc
    12121 tctgtgcaaa agagtgcaaa ccgagggagg tgaataatgg ctaaaacatt tgaagttcgc
    12181 ctttccggaa gtggtggtca gggcttaatc ctggcaggaa tcattttggc taaagcagct
    12241 gttttggaaa acagaaaagt aactcagacg caaagttacg gtcctgaatc ccgaggcggt
    12301 tattcgcatg ccgatgtgat tattagtgat aaggaaatat actttcctga agcaacgaat
    12361 attaattgcc tgctggcatt aactcaggaa gcatgcgata agtttctttt tgacttgtgc
    12421 gaaaacggaa tcctgattat tgatactacc ttcgtaaaaa atctggcttt agctgctgaa
    12481 aatacttacg aaatcccttt cacggaaatt gcccaggaaa aattaggcag ccctatttct
    12541 accaatatta tggcacttgc cttcctggta aaaattacag gtatcgtaaa agaaagctct
    12601 ctaaaaaaag caatagagga aacggtcaag ccagcatttg tagaccttaa tttgaaagca
    12661 atgcagattg gctttgacct ggcaaaaaaa taataaaaat aatgtgtagt cggaggacgc
    12721 tgttcctcca caaagataaa acccgaagcg aatatctgat aaaaaatgtg gagcttcagc
    12781 gagctccggc tacacgacta taacaatcat agagttagga gtcgttactg ccgcaagtaa
    12841 ttatgcgcaa ctcacaaatt gatttggcag tgaggactta tgacgactct aactactatt
    12901 tcataaagga gataacttat gcttaaacac atcagccata ttggtatcgc agttaaagac
    12961 ctggaagctg gaattgcttt ctacgaaaaa ttgggcttga aattagaagg cattgaagaa
    13021 gttccctctc aaaaggtgaa agttgccttc tttccttgtg gagaaacaag aattgaactt
    13081 ctctgcccta cttcggagga aagtcctatt gccaaattca ttgaaaagaa aggagaggga
    13141 attcagcata ttgcctttgc cgttgatgat attgaagctt ctttaaaaca ggcaaatgaa
    13201 ttaggtttag ccctaattga taaagaacct cgtccgggag cacatcactc caatattgca
    13261 ttcctgcatc caaaatccgc tttgggagtc ctgatagaat tttgccannn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnata ttgcattcct gcatccaaaa tccgctttgg
    13441 gagtcctgat agaattttgc caagaacacg ggtaaaggta aacgggtgaa caggtaaaac
    13501 acgcgttgga aatagagaat tagtaagata atgagatggt aagaattata aaagattttt
    13561 cttatatata aatcaaatcc tgtaaatcct caaaatcctg ttcatcctct acctattctt
    13621 ttatccgtgt aaatccctgt cctaatcttt tatctgtgtt atccgtgtta tctgtgagag
    13681 acatttcttt tgcgtgaaat ccttgacaga tttcccctct ccaaaaatct gcactcatat
    13741 atactattat aggagaaaaa atgtacgcca tcgtagaaat taaaggattt cagtataggg
    13801 ctgaaaaaaa cgctgtgctg cgcgttccct atctgaaaac ggcagaaccg ggtcaaacat
    13861 taacatttga caaagttctt ctcctgcgtg aggaagaaga aataaaagtc ggtcaacccg
    13921 ttgttgaagg cgctagcata gttgcagaag tgattgaaaa tggtaaggga gaaaaaaaga
    13981 ttatttacca tcataaacgt aggaaaggtt atcgcgtgaa aaaaggttat cgcgaaagtt
    14041 acacagacct ccgggtcacg gatattttgg cttaaaaaaa gaggttaaaa atggcacata
    14101 aaaaaggtgt gggaagttcc agaaatggcc gtaacagtaa tcccaagtat cgtggtgtaa
    14161 aaaaacacgc cagtgaaagt gttttagctg gtaatattat agttcgccaa aaaggtacaa
    14221 aaatacaccc cggtaaaaat gttgggatcg gcagggatta tactatttat agtttaatag
    14281 acggtaaggt tacttttact attggaaaag aaggcagaaa gtatgtgagt gtgcaaccga
    14341 tgaatgaaga atgaaaaatg tagaatgtaa aatggatttt gtaaggaaat tataaataac
    14401 tttcggtgat ttcggtgact aacaaaaaaa acataacttc taattaaatt cggtgttctc
    14461 ggtgtaaatg aaactataag gagaaaagat ggaagcgcaa caaattcttc tgcttatact
    14521 tagtatcata atcatcggaa ctaccattat cgtaggaatc actttatata aggatcaagc
    14581 atacacagcc aataaaactg ctcttgttgc agaagccaaa aattatggta agagaattac
    14641 caaatattat caagaccttg cttctttaaa gaatgataat ttacaaagtg cttctgtaga
    14701 tacaactaag ctgataaagt atcttggctg ggaaagcaat ttcataaaga cagaagcagg
    14761 aacttttaac attactgccg tttctgactc ttccgtaatt attacgggat atgcaaaagc
    14821 caaaaagaac ggcaaaagcc ctaaagttgt tgttactgtc actttcccgg aaggaaaaat
    14881 ggatctgagg gaaagtgact cagtaacgaa gtaaaagcaa aaagggtgat tgtgcttgac
    14941 ataaaaaggc aatctcaaga gttgaacaca ataaaaaaag tccggttttg gaggtaataa
    15001 tggaaatccg ggatgattta tattacacgg acacccacga atggatacat ttagatggcg
    15061 atatagctac aatcggtatc agtgattttg ctcagcatga gcttggcgat attgtttttg
    15121 tagagcttcc cgaagtagga caaaaagtag cagccagtga accctgtggt tcaattgaag
    15181 ccgtgaaagc ggtagaagac ctgatcagtc ctctaagtgg caaagttgaa gagaaaaaca
    15241 ttgacctgga agacagtcct gaactgatca ataaatcgcc ttacgaagaa gggtggttat
    15301 tcaaaattcg gatgtccaat ccggaagaac tggaaaatct gctttctgct gcagagtatc
    15361 agaaaataat tccctaatcc cttcagagta taatatcacc ttgtgattac gaaagacaat
    15421 cactttctta taatactttc tgctccttcc ggaggaggaa aaagtacgat cctaaacgaa
    15481 atcctgaaag ttactaacga tatagattat tccgtttctt acaccacgcg cagtccaaga
    15541 ggaacagaac aaaatggaat tcattatcac tttgtaaccg aagaggaatt tcagagacgc
    15601 attgacgagg gtgacttttt agagtatgca aaggttttcg gaaactggta tggcacttcc
    15661 aaaagtttta ttaaagaccg tttggcaaaa aagcatcatg ttattctgga cattgatgtt
    15721 cagggtgctt ctttaatcag tgccacggat attccctatg taaaaatatt tattttgcca
    15781 ccttctatgg aagtgttgca tcagcgttta gttttaagag ctacggatag tgaagaggaa
    15841 attgccaagc gactgcaaat tgccaaagac gaagttgctt atatacctca atacgactat
    15901 cttgtaatta acgataaatt ggatatagcg gttcaggatg ttttagctat tattcgggct
    15961 gaagaaaatc gtgtatgccg ctataatcat cccgctatgg attttatatc tacaggagaa
    16021 aaagatgatt aacgaaaaac ttgagaactt tctggaaaaa gcggatatga actatttgtt
    16081 ctgtctttta gctcaattgg aagcataccg cttactccag cttccaactt atgtgaaaca
    16141 aaaatttcaa gataaaatta ccgttatggc tatgaaccat gtagctgaaa atgaagtgcc
    16201 ggattatatt accgaacttg ctgaagcgga gctggcagaa gcagagcttg ctgaagcaca
    16261 ggaaagtcct ttccaggaag aagaaggggc agaagaagaa gcagaagaaa caattgatga
    16321 ttctaaaaag ttcattgaag agcttactgg aaccgaagat tatgaagagg aagaagagga
    16381 agaagaagaa ttctataatg aagataacga agacaacgac gaaagttaaa ttatgtctgt
    16441 aaatgctttg cgccaatatc tggaattctt aaaaaacagc ggtatcaagg aactttatta
    16501 tcccccaagc gataaacaaa aaaaattaac ggaactgcaa aaacaatatt ctacctgcac
    16561 aaaatgccct ctgcataatt cacgccataa atttgtttac ggggaaggaa atcctaatgc
    16621 cattgcaatg ttgattggag aaggtcccgg cgaacaggaa gacattacag gaagaccttt
    16681 tgtgggtgct gcaggtcagc ttttggaaaa aatgctttct gctattaatc tgaaacgcag
    16741 tgatgtttat attaccaata ttgtaaaatg tcggcctccg ggaaaccgaa atcccgaatc
    16801 cgaggaaaga aaagcttgtt taccctattt attggaacag attcaaatta ttcaacctaa
    16861 attactatta attttaggtt tggtggcagc gcagaccttg ctaaataata ataatactct
    16921 tggctggcat agaggaaaaa ttcattatgt tatggatatt cctgcttttg taacctacca
    16981 tccttcagct ttgttaagaa atcaggaatg gaaaaaacct gcctggattg acctccagga
    17041 attccagaaa gaatatgaga aattgcttgc tgcaactatt agctaaagga ttgccgaatt
    17101 atactgcaca aataatgtat atcgttttat gattgaacat acagattcct gatttcgcag
    17161 gaataacaag gatgcttaat gaaagaggga aaaaagcaat tattttattc tcataaggag
    17221 ataacgatga ttaaacagag aaattcccct ctctctattt tcaaactgaa tttacctata
    17281 tctcttctta taacttttct tttaactttt ttagttgtag gtttggatgc ccgttttgct
    17341 atttatggag atacacgcag taatccaaaa attcatcagc aaattgttca acaaattgta
    17401 tcccgcaagc ccgaaattgt ttttcatacg ggggatttga acagttcagg caaaacacaa
    17461 aaagagtatg acaatttttt gcagattatt tctcccatct ctcaatctgc ccgttttttt
    17521 ccagcgcgcg gcaatcacga aaaagacctt actttatttc tgcagaattt tccttattta
    17581 aacagcagtt cttattacac cgtttttcag gatggcatcc gttttatcat tctggatagt
    17641 gttttagagc tttccccggg ttccgaacaa tttagctggc tgcaaaaaca gcttaaagaa
    17701 gacattcctt caatgcttat tttacatcat ccggtttttt catccggaga gcacaatg
//
LOCUS       DAOPFL010000031        16121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_4109_length_16121_cov_91.473111, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000031 DAOPFL010000000
VERSION     DAOPFL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16121
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_16121_cov_91.473111"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLG69_03740"
     CDS             complement(<1..1084)
                     /locus_tag="PLG69_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPI42654.1"
                     /translation="MDEKFLYHIWDEGHLAPNLKTVSGKDLKVNYQGQFNTFRGPDFI
                     NAIISIDGEDLQGAVEIHKNTQDWIKHSHQEDVFYNQVILHIVLHNDSPLPFTMKENG
                     ELVEILELKNQLSDEIQKLLADIGDKNLASGSDYCDLLSAIDNDRLLSILTISGKQRF
                     MIKVRRFNASLALSDFDQILYEGMMEAAGYDKNKFNLFQLAQSIPFAKLKEWHKEGLK
                     SDEMISIFLGSSGLLLKSRNRLNGELYATLSHSFESQRFYARKINVDWQLFRIRPGNH
                     PVFRLILLSEFLYNCLQEGVLHFFLKTVELAKPNPQKRYQTFCKQFKPEQEGLLQNNP
                     GLGITVINNIYINIYLPILYLYYQKLA"
     gene            complement(1084..1755)
                     /gene="mtgA"
                     /locus_tag="PLG69_03745"
     CDS             complement(1084..1755)
                     /gene="mtgA"
                     /locus_tag="PLG69_03745"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPI42655.1"
                     /translation="MKRRRSIWLRIIRFIVIIHLCYWVIIAFFSLLYNFVNPPVTPLM
                     LQRYLVRGYPVHKRYYVPLEKIPERTQSMLIAIEDANFYKHFGFEWKMVKEAYQRNKK
                     AGKIRFGASTISNQLARTIFLTTNRSYLRKYLEIQATVIMEICMSKKRMLELYFNYVE
                     WGKGIYGIETASRYYYGKSCTNLTRTQTMKLISILTNPLRYTPETYYKSASARQRYNL
                     LQRYF"
     gene            complement(1812..2081)
                     /gene="rpsT"
                     /locus_tag="PLG69_03750"
     CDS             complement(1812..2081)
                     /gene="rpsT"
                     /locus_tag="PLG69_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPI42656.1"
                     /translation="MPQHKSPLKRMKTDKKRAARNNYVRNTIRTLSKKIHSDTTPEEK
                     EKLLSELYSKLDKAVKKGVIHKRTASRRKSRLADYVNKAKVEKTS"
     gene            2375..4432
                     /gene="fusA"
                     /locus_tag="PLG69_03755"
     CDS             2375..4432
                     /gene="fusA"
                     /locus_tag="PLG69_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPI42657.1"
                     /translation="MKDNEMKKIRNLLFIGASGAGKTTLAEQIFYLTKTTNRLGRIEE
                     GNTIMDFDPEEIAKNMSLGLSIGYVKYLDHKINILDTPGCPDFVGDAIVAIPAVENAI
                     IVANAAGGYEVGLELALEQIEERKMGKVFVVNRMDNDNADYDKTLEAIAENTDIKPVK
                     IYLPIGKEGAFEGVVDIIKRKAIKAGKESEVPTNLAEAVEEARIHLMEAVAETDEDLL
                     NEYLENMELPEDKLIEGLKKGILSGEISPAFVCSAGTGEGVLALLNGIINYLPSPAEA
                     NEIEVLKENEKTAFICQPDGELLGYIFKLYADPGMGDFVYFRLFSGTLKTGMEFYIPE
                     KDAKDKAGTMYFMLGKNRNDCNEIKAGDIGALLKLKNAKVMNSIVSLGSKISVIPVEL
                     PTPTTWQTIKAVNQADEDKIGTVLQRVIAEDPTIRYELNVETHENVLSGMGEQQLQLV
                     LKKLKNRYKVDAILKEPRIPYKETIMASAESNYKHKKQSGGRGQYAEVYFRIKPTERG
                     EGFKFINSIVGGVIPSNYIPAIEKGLWETMEKGIIAGYPVVDISVEVYYGSYHEVDSS
                     EIAFKIASSMALKDAFKKAKPILLEPIHELIIIVPSEYMGDVMGDISTRRGKILGMEQ
                     HSKKQYLTAQMPLAEMYSYYPALKSFTQGRGRFIQKFSHYEKVPDDIAQKVISAWQEQ
                     TNE"
     gene            4774..5526
                     /locus_tag="PLG69_03760"
     CDS             4774..5526
                     /locus_tag="PLG69_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPI42658.1"
                     /translation="MSGHNKWSSIKHKKGAADAKRGQVFTRLVKEIILAAKTGGGDPD
                     MNPRLRTAINTAKENNMPKENIERAIKRGTGEIEGAAYEEVIYEGYGHNGVGIVVEVM
                     TDNKKRSVSELRHTFSKYGGNLAETGSVAWNFEQKGFFNVPSAGLDEDEFMLKALEAG
                     ADDVELNDDNFDVYTSPADFHIVIGNLEKEGYPIHNAELTRVPKNTIAVDDDTAEKLM
                     RLIESLEELDDVQKVYANFEVSDEVMEKLSQA"
     gene            5535..6014
                     /gene="ruvC"
                     /locus_tag="PLG69_03765"
     CDS             5535..6014
                     /gene="ruvC"
                     /locus_tag="PLG69_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPI42659.1"
                     /translation="MIIIGFDPGSRYCGYGLLQIESRKVLAAGCDVIDVTRGKTLALR
                     LKSLYENIMQILEEYHPEIAAVESIFFHRQVRSVFTLGHSRGVILLALAMQEIPVVEY
                     SPREIKKSVCGNGNASKQQVRYMINQLYPLKESPKRDDAFDALAVATCHYNRMKWLQ"
     gene            6011..6610
                     /gene="ruvA"
                     /locus_tag="PLG69_03770"
     CDS             6011..6610
                     /gene="ruvA"
                     /locus_tag="PLG69_03770"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPI42660.1"
                     /translation="MIQYISGTLTFKTPVLAIIETMGIGWELKIPISTYEKLPTAGKP
                     CKLLTYLHISQDDIRIFGFATEAERELFVMLNKIGGIGPKIALSILSTLSIPTFIKAV
                     NSGEEGLLTKVPGIGKKSAQRLIVELKDDVHKLLSHIDQKELQIGDVSSEVESALLSL
                     GFNLHQIRKELALMDESMQKLNTEELIKEIIKRIYQRNK"
     gene            6607..7923
                     /gene="rsmB"
                     /locus_tag="PLG69_03775"
     CDS             6607..7923
                     /gene="rsmB"
                     /locus_tag="PLG69_03775"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPI42661.1"
                     /translation="MNFRSEAYTTILKVLKDKEYSDTLLHQRAKKLKNAGEDPALFYN
                     LVKGVIKLYLKLDYILQQYVDKKKYTDTDIKIKVLLYMGLYQLLYLNSIPDYAAVNET
                     VELAKTLFNPQVADFVNAVLRNYLRNPEVSYPEEPALRIAYEHSFPPELIKQWLDLWG
                     EENTEYLAMFFNENPELHIRVNSTATTPEKLKAYFAKRNIVITPSQASPMMFHTAQTA
                     EVLEDVAFSEGYFSVQDTSAALIVELLDPQKEESVLDLFAAPGGKCSYIAERMDNTGE
                     VIAVDKIPSKMKLLKQAANRLQLTNIKMVVTDAFKYGPVAPAFHRVLVDVPCSGWGVF
                     GRKSDLRWQAHQNIPELIKLQEKALDYAANFVAPGGFLVYSTCTMNPKENEDQISAFL
                     KKNRNFELIPADAFIPERYTYAGYLKTIPFKHNMDGAFAAKLQRRK"
     gene            7886..8518
                     /locus_tag="PLG69_03780"
     CDS             7886..8518
                     /locus_tag="PLG69_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPI42662.1"
                     /translation="MVLLQPNYNAGNKNIIVRSKSPTGTKKAGYIIGIGIGIIIVTAF
                     VFSQILFPVILGRTPKVEVPSVVGMTLVQAKRVLQEEKLHVIVKDSFFSDVAAAEVVL
                     EQNPVAGEKIRQDGTVYLVISKGSSTVTLPSLIGKPFQEAFIILKNLDLYSSVVDSTY
                     SDIYPVNTVIRSIPAAGEKALKKSTVKLILSRGPEPVADTLNYEIPIYYY"
     gene            8543..8998
                     /locus_tag="PLG69_03785"
     CDS             8543..8998
                     /locus_tag="PLG69_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42663.1"
                     /translation="MKETLKEAKIRSRMMPGALTITGFLGTDQRPLAEIIAADEQELK
                     KLNRSVEEIAERMKYFSNATFDAYNNTVVIDDTYEVHTESTKGTMTCPYGHGGLYRKY
                     VTTLTNRKNNITVRWNAIDIHLIEAHHFFEGKGSPFRLEPEILVKALFS"
     gene            complement(9141..10349)
                     /locus_tag="PLG69_03790"
     CDS             complement(9141..10349)
                     /locus_tag="PLG69_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPI42664.1"
                     /translation="MKILLITYYFPPCGGASVQRWLRFIRALTKKGVDIYVVTTLNGD
                     YPYLDESLVAKIPSSVMVYRSKPLRFGKLWNILGQKELPYGSLQNKASDSPLKKILYW
                     LRLNFIVPDMRIGWNPSAYKNALQILRQDKIDYVITTGPPHSTHLVGMKLKKHFGIQW
                     RTDFRDPMSEIYYLKINPPCRITLALHKYLERKIIRTADLNYIFSKVIADALPDGKKK
                     VLYNGFDPDDFKGLHYSRTDKFRIKYVGQLTTGRDASPLMKALSSLQLPLLEFSLIGT
                     RDFPETDFPVIKIPFLPHSKALEELVNAELLILIIDTCKSKEGLLPSKLFEYMASRTP
                     ILCISEPAGEVYTLICKTESGFVSKDSEEIANYILGLYNKFLEGDNLRTKGDISFVDV
                     NNQVNIFLEG"
     gene            complement(10346..11431)
                     /locus_tag="PLG69_03795"
     CDS             complement(10346..11431)
                     /locus_tag="PLG69_03795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPI42665.1"
                     /translation="MKIAILGPAPPFRGGISRFALSLARAFKDTGHNVQFFNFREQYP
                     KLLFPGKGQYDSELPTNEFVNHQILTPWLPKTWKATIHELKIYRPEAIIVSWFLPYFA
                     PAYGYILRQITCSRKVILAHNIASHEKWLGEKYFLQYAFKPATKIVTLSKATLDELHR
                     KLPLYISKKGVSGFHPCYDMYNDNISATRKDNTLFFFGLIKPYKGLDILLQAMPKVIE
                     AFPDVKLIIAGEVYGNKNIYTEQIQALGIENNVECYFRYLGDPEISYFFNQSCLCILP
                     YKSASQSGVIATSYSFGVPVLATKVGGLGEYVIEGKTGYLVPPDNPSALAEAIINHLQ
                     QKPDMSQAINEYTKRYSWKGLAELLIK"
     gene            complement(11428..12405)
                     /locus_tag="PLG69_03800"
     CDS             complement(11428..12405)
                     /locus_tag="PLG69_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPI42666.1"
                     /translation="MKKNILIILTGGTIAMKVSDPAGVIPSPEFAEFLRQFPQLNSVA
                     NVEVMDYLNIPSPYMTPEMMFELAELIDLKIIDYDGVVITHGTDTLEETAFLCDLVLT
                     TRKPVIFTAAMRSGDEIGLDGPRNIIGSVRVASHPESIDKGVLVVMNDEIHTARDVVK
                     ADTGKIDAFVSPGYGPIGNVDPDKIVYHRASLYRENVWTEQLDTRVELIKAVAGMDGK
                     FIRCCLEHDIKAIVIEALGRGNLPKTLVPDIEEAIARNVLVIITSRTYTGRVLPEYGY
                     EGGGKHLESIGCILAGDMKGVKARLKLMALFGKYQDADIVKRFFQQSLL"
     gene            complement(12523..13191)
                     /gene="queC"
                     /locus_tag="PLG69_03805"
     CDS             complement(12523..13191)
                     /gene="queC"
                     /locus_tag="PLG69_03805"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPI42667.1"
                     /translation="MTRAIVLLSGGMDSLVTAACAVSECDEVCFLHFNYGQLTEKKEL
                     SSFEALVNYYQPTQAKVINYSWLSEIGGSALTDRNIPVQKGILSGSKIPITYVPFRNA
                     IFLCSAVAWAEVIQAARIYIGAVEEDSSGYPDCREVFFQAFNKVIATGTKGEFPIEIS
                     TPVLHKSKAEIVQLGIELKAPFELSWSCYTENDVACGVCESCLLRKKAFKTAGVEDPL
                     PYRN"
     gene            complement(13293..14702)
                     /gene="hydG"
                     /locus_tag="PLG69_03810"
     CDS             complement(13293..14702)
                     /gene="hydG"
                     /locus_tag="PLG69_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPI42668.1"
                     /translation="MKINTEGLKSFIPTAEIEEHLNKNRFADAIRIRDIVDKSLDKQR
                     LNPDETAALINVTDPELKNLILEGAHTLKERIYGNRIVLFAPLYVGNECINDCVYCGF
                     RISNKECQRATLSKEELIAETEALEDKGHKRLIMVYGEHPKYSPEFIAETVQTVYNVK
                     HNKGEIRRVNINAAPLDIEGFRTVKSVGIGTYQIFQETYYEETYKNLHPRGPKSNFLW
                     RLYGLDRAMQAGIDDLGIGALMGLYNWRFEVMGLLYHTIHLEERFGVGPHTISFPRIE
                     PAIGTDFTKNPPYKVSDEDFKLLIASIRLSVPYTGMILTAREPIAVRDEVLRYGISQI
                     DAGSSIGVGDYAHKDEESKRKSQFVLGDTRTLDDVIYELAHSGYIPSFCTSCYRAGRT
                     GEHFMEFAIPGFVKRFCTPNALLTFAEYLYDYASERTKKIGLELIDKEVAKIDDPNMQ
                     SSVQENLAKMKEGIRDLHY"
     gene            complement(14692..14964)
                     /locus_tag="PLG69_03815"
     CDS             complement(14692..14964)
                     /locus_tag="PLG69_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HPI42669.1"
                     /translation="MNTKYHILSIIMEDRESAFHPVNELLHSYAQNILLRVGYPMRDL
                     GVAVIFLIVELSVSEMGAFSGKLGQIKSVKVKVTTLKLEEGEQNEN"
     gene            complement(15603..>16121)
                     /locus_tag="PLG69_03820"
     CDS             complement(15603..>16121)
                     /locus_tag="PLG69_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HPI42670.1"
                     /translation="LCGIEIGDYHRVKEYALSLVEDINFDLILGSVHFLSDATNVAVP
                     LKKPLSEEQVRDYYQQNLLLVSHCNIDVLAHLGVYKRGYNYIPDESAFYPLLKDIFNT
                     MIDRKIALEINYSSLCRGYPSFLPELPLLEMFLDLGGRYFSVGSDAHKIEHFDLYREL
                     IPTQFCTSFRYL"
BASE COUNT         5201 a         2987 c         3108 g         4825 t
ORIGIN      
        1 ttgccagttt ttgatagtaa agatagagaa tgggcagata aatattaatg taaatattgt
       61 tgataaccgt aatacccaaa cctggattat tttgcaacag tccttcctgc tctggtttaa
      121 attgtttaca aaaggtctga tatctttttt ggggattggg tttggctaac tctactgtct
      181 tgagaaagaa atgcaaaacc ccttcctgta aacagttata aagaaattca cttaatagaa
      241 taagacgaaa aacaggatga tttcccggac ggatacgaaa aagttgccag tccacattaa
      301 ttttacgcgc ataaaatctc tggctttcaa aagagtggga aagagttgca taaagttcac
      361 cgtttaaacg attccgactt ttcagtaata aaccggaaga accaagaaaa atactgatca
      421 tttcatcact tttcagccct tctttgtgcc actctttcaa tttggcaaag ggaatgcttt
      481 gtgccagttg aaagagattg aatttattct tgtcgtatcc tgcagcttcc atcattcctt
      541 catagaggat ttggtcaaaa tcgcttaaag ccaaagaggc attaaatcgg cgcactttaa
      601 tcataaatcg ttgttttccg gaaatggtta aaatgcttag taatctatca ttatcaatgg
      661 cagaaagaag atcgcaataa tcactgccac tggctaagtt cttatcgcca atatcagcca
      721 gcagtttttg aatttcatcg gaaagctgat ttttcaattc cagaatttct accagttcac
      781 cattttcttt catagtaaaa ggtaaagggc tatcattatg caagacaata tgtaaaataa
      841 cctgattgta aaatacatct tcctgatgag agtgtttaat ccaatcctga gtgtttttat
      901 gaatttccac cgctccctgt aaatcctctc catcaataga tattatggca ttaataaaat
      961 cgggaccccg aaaagtattg aattgaccct ggtaatttac tttcaggtct ttaccgctaa
     1021 ccgttttcag gtttggagcc aagtgacctt catcccagat atgataaaga aatttttcat
     1081 ccattagaaa tacctctgca gcaagttata tctttgcctt gcggaagctg atttataata
     1141 tgtttcagga gtatagcgaa gaggattggt taaaatgctg atcaatttca ttgtttgcgt
     1201 ccgggttaaa ttcgtgcagc ttttgccata gtaataacgc gatgcagttt caatgccata
     1261 aattccctta ccccattcaa cataattgaa atagagctcc agcattctct ttttactcat
     1321 acaaatttcc ataatcaccg ttgcctgaat ttccaagtat ttacgcagat aactcctatt
     1381 tgtggttaag aaaatagttc tggcaagttg attactgata gtactggctc caaaacgaat
     1441 ttttccggct tttttattac gctgatatgc ttctttcacc attttccatt caaagccgaa
     1501 atgcttgtaa aaattggcat cctcaatagc aataagcata ctttgggttc tttcaggaat
     1561 tttttccagg ggaacataat accttttatg aacaggataa ccacgcacca gatatctctg
     1621 caacattaaa ggtgtgacag gaggattaac aaaattatac aaaagcgaaa aaaatgctat
     1681 tatcacccaa tagcaaagat ggataataac aataaaccta ataatacgca gccagatgga
     1741 acgcctgcgt ttcaagaatg atccttattg cctaaaacta ttttgcttgt ttataatgcc
     1801 tgaacactaa ttcaagatgt cttttctacc ttagctttat taacataatc agctaaacga
     1861 gattttctgc gagaagccgt tcttttgtga ataacacctt tcttgacagc tttatcaagt
     1921 ttggaataga gttcagaaag cagtttttcc ttttcttcag gagtggtatc gctatgaatt
     1981 tttttggaaa gagttctaat agtgtttctt acataattat tgcgagcagc cctctttttg
     2041 tctgttttca ttcttttcag cggggacttg tgttgtggca taatatcctc tcttttttta
     2101 atataagtat ttatttggca cacataaaaa tgcccttcat ttctgtcaag aaaattgtga
     2161 ttggcaatta gagtgaaaaa agcgaaatag cgatatggat tttactctct atagaatatc
     2221 tcctgttttc cctttttgct gctattacga atttttcagg tttaattgtc atcatagaga
     2281 aaaaagggat tgacggaaac agcttataaa aaattgtgac ttaccaatat aaaacaatat
     2341 acaaaaaata tatgataaac gaactgagga aaagatgaaa gacaatgaaa tgaagaagat
     2401 tcgtaatcta ttgttcattg gagccagcgg tgcaggaaaa acaaccctgg cagaacagat
     2461 attttactta acgaaaacga caaaccgctt gggcagaatt gaagaaggca atactataat
     2521 ggattttgat cctgaagaaa tcgctaaaaa tatgtccttg ggtctttcca ttggttatgt
     2581 taaatatctt gatcataaga taaacattct ggatacacct ggttgtccag atttcgttgg
     2641 agatgcaatt gtagctatac ctgctgtgga aaatgctata atcgtagcta atgcggcagg
     2701 tggttatgaa gttggtttgg aacttgcctt ggaacaaatt gaagaacgca aaatgggtaa
     2761 ggtgtttgta gttaaccgaa tggataatga taatgccgat tacgataaaa ctttggaagc
     2821 aattgctgaa aataccgata ttaagccggt taaaatttat cttcccattg gcaaagaagg
     2881 tgcttttgaa ggtgttgtag atattattaa acgaaaagca attaaagccg gaaaggaaag
     2941 tgaagttcca accaatttgg cagaagcagt ggaagaagca agaattcatt taatggaagc
     3001 tgttgccgaa accgatgagg atttgctgaa cgaatatctg gaaaatatgg aactgccaga
     3061 agataaactt attgaaggac ttaagaaagg aatcttaagc ggtgaaatat ctcctgcctt
     3121 tgtttgttcc gccggaactg gagaaggagt tttggctctt ctgaacggta ttataaacta
     3181 tcttccttct cctgccgaag ccaatgaaat agaagttctt aaggaaaatg agaaaaccgc
     3241 ttttatttgc caacccgatg gtgaactttt gggttatata ttcaaactct atgccgatcc
     3301 gggaatggga gattttgttt atttccgtct tttttccgga acactaaaga caggtatgga
     3361 attttacatt cccgaaaaag atgccaaaga taaagctgga acaatgtatt ttatgttggg
     3421 gaaaaatcgt aatgactgca acgaaattaa agcaggagat attggcgctt tactgaaact
     3481 aaaaaacgcc aaagttatga acagcattgt ttctcttggt tctaaaatat ctgtaatccc
     3541 ggtagaatta cctaccccaa ctacctggca aaccatcaaa gctgttaatc aagccgatga
     3601 agataaaata ggcactgtcc tgcaaagagt gattgctgaa gacccaacca ttcgctatga
     3661 attaaatgtg gaaacccatg aaaatgttct atccggaatg ggagaacagc aattgcaact
     3721 ggtgctgaaa aaactgaaaa accgttataa agtagatgcc atcctcaaag aaccgcggat
     3781 tccctataaa gaaacgataa tggcaagcgc agaatccaac tataaacata aaaaacagtc
     3841 aggtggccgc ggtcaatatg ctgaggtcta tttccgtatt aaaccaacag aaaggggaga
     3901 aggattcaaa tttatcaatt ccattgtagg cggagtaatt ccctccaatt atattcctgc
     3961 aattgagaag ggactttggg aaacaatgga aaagggtatc attgccggct accctgttgt
     4021 tgatatttcc gtggaagttt attatggcag ttatcacgaa gttgatagct ccgaaattgc
     4081 ttttaaaata gcttcctcaa tggctttgaa ggatgctttc aaaaaagcca aacctattct
     4141 tttggaaccc attcatgaat tgattataat cgttccgagt gaatatatgg gtgatgttat
     4201 gggtgatatt tctacccgga gaggtaaaat tctgggtatg gaacaacaca gtaaaaaaca
     4261 atatttaaca gctcaaatgc ctttagcgga aatgtattct tactatccag ccctgaaatc
     4321 attcacccaa ggtagaggac gttttatcca aaaattctca cattatgaaa aagtgccgga
     4381 tgatattgct cagaaagtga tttctgcctg gcaggaacaa accaacgagt agagtgaacg
     4441 gtgaacggtg aacagtgaac ggtgaactgt aaaaagggga aaccagagat tgaataacgg
     4501 aagaatgatg ttctcatcgt tcatttataa ctaatctctc gctttattgc aaactaagaa
     4561 agcccaatgt tccttactca tggttgacga ggataacaac cttccagaat ttgtgaaaca
     4621 cgattgattg tgtatacaga ggaagccgtt catccgtaaa aaatattcag agaaatggca
     4681 aactccgttt ccacttttcc tttttttctt gacaataaag gactgctttt cataatgatt
     4741 ttgtaagatg ataaagaaca aattgaggtt tatatgtctg gacacaataa atggagttca
     4801 ataaagcaca aaaaaggtgc tgctgatgca aaaagagggc aggtctttac ccgcttggtt
     4861 aaagaaatta ttttagcggc taaaaccgga ggtggagatc ctgatatgaa tccccgctta
     4921 cgaaccgcta ttaatactgc caaagaaaac aatatgccca aagaaaatat tgaacgtgca
     4981 attaaaaggg gaacaggtga aattgagggt gccgcttatg aagaagttat ttatgagggc
     5041 tatggtcata acggagtagg aattgttgta gaagtgatga ccgacaataa aaaacgcagt
     5101 gtttctgaac tcaggcatac attttctaaa tatggaggca accttgcaga aacggggtcg
     5161 gttgcctgga attttgaaca gaagggattt ttcaatgttc cttctgccgg tttggatgaa
     5221 gatgaattta tgcttaaggc actggaagca ggagctgacg atgttgaact taatgatgat
     5281 aattttgatg tttatacttc tcctgctgat tttcatattg ttatcggtaa tctggaaaaa
     5341 gagggctatc ctatccataa tgcagaactt accagagtgc ccaaaaatac aattgccgtt
     5401 gatgatgata ctgcagaaaa actaatgcgc ttgattgaat ccctggaaga gcttgatgat
     5461 gtgcaaaagg tctatgccaa ttttgaggtt tcagacgaag ttatggagaa actttctcag
     5521 gcataaataa ctaagtgatt attattggtt ttgatcccgg cagccgttat tgcggttatg
     5581 gtttacttca aatagaaagc agaaaagtgc ttgctgccgg ctgtgatgtt attgatgtta
     5641 cgcggggaaa aacattggca ttaaggttaa aatcactcta tgaaaacatt atgcaaattc
     5701 tggaagaata ccatcctgaa attgcagctg tggaaagcat tttcttccat cggcaggtga
     5761 gaagcgtatt tactttaggt cattcacgcg gtgttatttt acttgcttta gcaatgcaag
     5821 aaattcctgt tgtggaatac agtccccggg aaataaaaaa atccgtttgc ggcaacggca
     5881 atgccagtaa acaacaagtt cgctatatga taaatcagct ttatccatta aaagaaagcc
     5941 ctaaaaggga tgatgctttt gatgccttag ccgtagcaac ctgtcattat aaccggatga
     6001 agtggttaca atgattcagt atatttccgg aactcttacc tttaaaacac ctgttttggc
     6061 tattatagaa acaatgggca tcggctggga actgaaaata ccgattagca cttatgaaaa
     6121 attgccaact gctggtaaac cctgtaaatt gcttacttat ttgcatatca gtcaggatga
     6181 tatacgcatt tttggctttg ctacggaagc agaaagagaa ctttttgtta tgttgaacaa
     6241 aatcggcgga attggtccta aaattgccct atcaatactt tccacgctaa gcattcctac
     6301 ttttataaaa gctgtcaatt ctggagaaga aggtctattg acaaaagttc cgggtatcgg
     6361 gaaaaaatct gctcagcgtt tgattgtaga actgaaagat gatgtccata aattacttag
     6421 ccatatagac cagaaggaat tgcaaattgg ggatgttagc agtgaagtgg aatcggcact
     6481 tttgtctttg ggatttaatt tgcatcaaat ccgtaaagaa ctggctttaa tggatgagtc
     6541 aatgcaaaaa ctaaataccg aagaattaat taaagaaatt attaaacgca tctatcagag
     6601 aaataaatga acttccgctc cgaagcatat acaactattc tgaaagtgct taaagataaa
     6661 gaatattctg atactttatt acatcagaga gctaagaaat taaaaaatgc aggagaagac
     6721 cctgctctct tttacaacct tgtgaaagga gtgatcaagc tctatctgaa actggattat
     6781 atccttcagc aatatgtaga taagaagaaa tacaccgata ccgatattaa gattaaagtc
     6841 ctgctctata tggggcttta tcagcttttg tatctcaatt ccattccaga ttatgctgca
     6901 gtaaatgaga ccgtggaact ggctaaaacc ttattcaatc cccaagtggc agattttgtt
     6961 aacgctgtgc tcagaaatta tttgcgcaat ccggaagttt cttatcctga ggaacctgct
     7021 ttgcgtattg cttatgaaca ttcttttcca ccggaattga ttaaacagtg gttggattta
     7081 tggggtgagg aaaatacgga atacttggct atgtttttca atgaaaatcc ggaactgcat
     7141 attagagtaa acagcactgc tacaactcct gaaaagctga aagcatattt tgccaaacgc
     7201 aatattgtaa tcactccttc tcaagcgagt ccgatgatgt ttcatacagc tcaaacagcc
     7261 gaagttttgg aagatgtagc attctccgaa ggatattttt ccgtgcagga tacttctgcc
     7321 gcactaattg tagaattgct tgatccccaa aaagaggaaa gtgttttaga cctttttgct
     7381 gctccgggtg gaaaatgcag ctatattgcc gaaagaatgg ataatacagg tgaagttatt
     7441 gctgtggata aaattcccag taagatgaaa ctgttgaaac aggcagcaaa ccgtttacag
     7501 ctaactaata tcaaaatggt tgtaacagat gcctttaaat acggtcccgt ggctcctgct
     7561 tttcacaggg ttttagtaga tgtgccttgt tccggatggg gtgtttttgg aagaaaatca
     7621 gacctccgtt ggcaggcaca tcaaaatatc cccgagctta taaaacttca ggaaaaagca
     7681 ttggattatg cagccaattt tgttgctccg ggtggttttt tggtttattc tacctgcaca
     7741 atgaatccca aagaaaacga agaccaaatt tctgcctttt taaaaaagaa caggaatttt
     7801 gaactgattc cagcagatgc atttattccg gaaagatata cctacgccgg atacttgaaa
     7861 acgatacctt tcaaacataa tatggatggt gcttttgcag ccaaattaca acgccggaaa
     7921 taaaaatatt attgttcggt caaaatcccc aacgggcact aaaaaagccg gttatataat
     7981 tggaatcgga attggaatta taatcgtaac agcttttgtt ttcagccaga ttctctttcc
     8041 tgttatttta ggaagaactc ccaaagtgga agttccttcc gtagtaggaa tgacccttgt
     8101 tcaggcaaaa agagtacttc aggaagaaaa attgcatgta atagttaagg actctttttt
     8161 tagcgatgtt gcagctgcgg aagttgttct ggaacagaat cctgttgcag gagaaaaaat
     8221 acgccaggat ggaacagttt atctggttat cagtaaaggt tcttctacag taactttgcc
     8281 ttcattgatt ggtaaaccct tccaagaggc atttataatt ttaaaaaatt tggatttgta
     8341 tagcagtgtg gtggattcca cctattccga tatttatccg gttaacactg ttatcagaag
     8401 tattcctgct gccggggaaa aagctttgaa aaaatctact gttaagctaa ttttaagcag
     8461 aggaccagaa ccggtagcag atacacttaa ttatgagata ccaatatatt attattaaaa
     8521 aacttaggat gggaaaagca caatgaaaga aacactcaaa gaagcaaaaa ttcgctcgcg
     8581 tatgatgcca ggagccctta ctataacagg ttttctggga acagatcaac gccctcttgc
     8641 agaaattatt gctgcagatg aacaagaact aaaaaaactt aatcgcagtg tggaagaaat
     8701 tgcagaaagg atgaaatatt tctccaatgc aacttttgat gcttacaaca acacagtcgt
     8761 tattgatgac acctatgaag tgcatactga atcaaccaaa ggaacaatga cctgccctta
     8821 tggacatggg ggactttaca ggaagtatgt aacaacatta actaatcgca aaaataacat
     8881 tactgttaga tggaatgcta ttgatatcca tctaattgaa gctcatcatt tctttgaagg
     8941 gaaaggttca ccgtttcgct tagaaccgga aattctggtt aaagcgttat tttcctaatt
     9001 ccgacaaatg tttagccaga ggccactgtt tctctgcaag aaaaagatgc ggagctacag
     9061 cgagctctgg ctacacgatt ccggaaaaac gatgtcctcg tcgttttata taaagtcgta
     9121 atttgtagtt gatggggaca tcaaccttcc agaaaaatgt ttacttgatt gtttacatct
     9181 acgaaagaga tatctccttt tgtgcgtaag ttatctcctt ctaaaaattt gttatataac
     9241 cccaaaatat aattagctat ttcttcacta tctttgctaa cgaacccgct ttctgtttta
     9301 cagattaatg tatatacttc accagccggt tcggaaatac aaagaatcgg agttcgggaa
     9361 gccatatatt caaaaagttt ggagggtagt aaaccttctt tacttttaca ggtatcaata
     9421 atcagtataa gcaattcagc attcaccaat tcctctaaag ctttgctatg tggtaaaaaa
     9481 ggaattttta ttaccgggaa atcggtttcg ggaaaatccc tggtgccgat aagagagaat
     9541 tccagcaaag gtagttgcaa cgaagaaagt gctttcatta aagggctggc atcccgtccg
     9601 gttgtaagtt gacctacata tttgatgcga aatttatctg ttcttgagta atgcaaaccc
     9661 ttaaaatcat cagggtcaaa gccattatat aagaccttct ttttgccatc gggtaaagca
     9721 tctgcaatta ctttactgaa tatataattc aggtcggctg tccggataat tttccgttcc
     9781 agatatttat gcaaagccag agttatacgg cagggaggat ttattttgag atagtatatt
     9841 tccgacatag ggtcccgaaa atccgttctc cactgaatgc caaaatgttt tttcagtttc
     9901 atacctacta aatgtgtaga gtggggagga cctgtagtaa taacatagtc aattttatcc
     9961 tggcgtaaaa tctgcagtgc atttttataa gcagaaggat tccagccgat gcgcatatct
    10021 ggaacaataa aattcaaacg cagccaatat aaaatctttt ttaagggact gtcgcttgct
    10081 ttgttttgga gtgaaccgta aggcagttct ttttgtccta atatattcca caactttccg
    10141 aatcttaatg gtttactgcg ataaaccatt accgaagaag gaattttagc cactaagctt
    10201 tcatccaggt aaggataatc accatttaga gtagttacta cataaatatc tacccctttt
    10261 ttagttaaag cacgaataaa acgcagccat ctttgaacag aggcacctcc gcaaggagga
    10321 aagtaataag tgataagtaa aatcttcatt tgatcagtaa ttccgccaaa cctttccagg
    10381 aataacgctt tgtgtattca ttaattgcct ggctcatatc cggtttttgt tgcagatggt
    10441 taattattgc ctcagccaaa gccgaaggat tatcaggagg gacaagataa ccggttttac
    10501 cttcaataac atattcaccc aatccaccaa ccttagttgc cagaacagga actccaaaac
    10561 tgtaggaagt agctataaca ccactttgtg aagcactttt atagggtaga atacagagac
    10621 aggactgatt gaaaaaatat gatatttccg gatcgccaag gtaacgaaaa tagcattcta
    10681 cattattttc aatacctaat gcctgaatct gttcagtgta gatattttta tttccataga
    10741 cctctccggc aattattaac ttcacatcag gaaacgcttc aatcactttg ggcattgctt
    10801 gcagtaaaat atcaagacct ttatagggct taatcagacc gaaaaaaaac agggtattat
    10861 ctttccgggt agccgatata ttgtcgttat acatatcata gcagggatga aaacctgaaa
    10921 cacctttttt agatatatac agcggtaatt tacgatgcag ttcatccaga gtagctttac
    10981 ttaaggtaac aattttagtt gccggcttaa aagcatattg cagaaaatat ttttcgccca
    11041 accatttctc gtgagaggca atattatgtg ccagaattac tttacgagaa caggttattt
    11101 gtcgtaaaat atagccataa gcaggtgcaa aatagggtaa aaaccaggag acaataattg
    11161 cctcgggtct atatattttg agctcgtgaa tggttgcttt ccatgtttta ggtaaccagg
    11221 gggtaagaat ttgatgatta acaaattcat tggtaggaag ttctgaatca tattgtccct
    11281 ttccagggaa aagcaacttg ggatattgct ctctaaaatt gaaaaattga acattatgtc
    11341 ctgtatcttt aaaagctctg gcaagggata aagcgaaacg ggaaattccg ccacggaaag
    11401 gaggtgcagg acccaaaatg gcaattttca tagcaaactc tgttggaaaa agcgctttac
    11461 aatatcagca tcctgatatt taccgaaaag tgccatcaat ttcaagcgtg ctttaacccc
    11521 tttcatatcc ccggcaagaa tgcaacctat tgattccaaa tgttttcctc cgccctcata
    11581 accatattca ggcagaaccc ttcctgtata agttctggaa gttattatca ctaaaacatt
    11641 tctggctata gcttcttcaa tatcgggaac cagagtttta ggtaaatttc ctcttcccaa
    11701 tgcttcaatc acaattgctt ttatatcgtg ttccagacaa cagcgaataa attttccatc
    11761 cattccggct actgctttga taagttcaac tcttgtatcc aattgttccg tccaaacatt
    11821 ttcccgatag agagaagcac gatgatatac aattttatcg gggtctacat tgccaatagg
    11881 tccataacca ggagaaacga aggcatcaat tttacccgta tcggctttca cgacatcgcg
    11941 agctgtgtga atttcatcat tcattactac caaaacacct ttatcaattg attccggatg
    12001 actggctacg cgaacactgc caattatgtt acgaggacca tccaaaccga tttcatcacc
    12061 ggaacgcatt gctgcagtaa aaataacagg tttgcgggta gttaaaacta aatcgcataa
    12121 aaaagccgtc tcttccaaag tatcggttcc gtgagtgata actacaccat catagtcaat
    12181 tattttcagg tctattaatt cagccagttc aaacatcatt tcgggagtca tataaggact
    12241 gggaatattc agatagtcca tcacttctac attggcaaca ctgtttaatt ggggaaattg
    12301 ccttaaaaat tcagcaaatt cggggctggg aattactccg gcaggatcag atactttcat
    12361 agcaatcgtt ccaccggtta agataatgag gatgtttttt ttcataatac ctaattttcc
    12421 gtttatatca tttcttgaag gaaaagtcct ttgtcattcc tgagaaagca ggaatccacc
    12481 gaagagaaaa gaatattata cattttcaac ccgaaattaa tgttagttac gatagggaag
    12541 aggatcttcc acacctgctg ttttaaatgc ctttttgcgt aagagacagc tttcacaaac
    12601 gccacaggca acatcatttt cggtataaca gctccaggat aattcaaaag gagctttaag
    12661 ttcaattccc agttgcacta tttctgcctt gcttttatgt aaaacagggg tgctaatctc
    12721 tatcggaaat tcaccttttg ttcctgtagc aatcacttta ttgaatgcct gaaaaaaaac
    12781 ctcacggcaa tccggataac cggaactgtc ttcttccaca gctccaatat aaattcttgc
    12841 tgcttgaata acttctgccc aggcaactgc agaacaaaga aatatggcat tccggaaagg
    12901 aacataggta ataggaattt tagaaccgga aaggattcct ttctgaacgg gaatgtttct
    12961 atcggttaat gcagagccgc caatctcaga aagccaggaa taattaatca ctttggcttg
    13021 agttggctga taataattta ccagtgcttc aaaagaggat aattcctttt tttccgttaa
    13081 ctgaccataa ttaaaatgca gaaaacagac ctcgtcacat tcagatacag cacaagcagc
    13141 tgtgaccaaa gaatccattc ctccgcttaa taaaacgatt gcccgggtca taaactttat
    13201 cctgattagt aatttttaca attttatgtt aataatgctt tatgtcaagt taagaatgaa
    13261 ttttcacccg gtcatctgtt aacttgttaa ccttaatagt gcaaatctct tattccttct
    13321 ttcatttttg ccagattttc ttgcactgag ctctgcatat tgggatcatc tattttagct
    13381 acttctttgt caatcagttc caagccgatt tttttagtgc gttcgctggc ataatcataa
    13441 agatattccg caaaagtgag taaggcattg ggagtgcaaa atctttttac aaaaccagga
    13501 atggcaaatt ccataaaatg ttctccagtt cttcctgcac ggtaacagga agtgcaaaaa
    13561 gaaggtatat aaccgctatg ggcaagttca taaataacat catccaaagt gcgggtgtca
    13621 cctaaaacaa attgactttt gcgtttggat tcttcatctt tatgagcata gtcaccaact
    13681 ccgatactgc ttccggcatc aatttgtgaa atgccatagc gtaagacctc atctcttact
    13741 gcaattggtt ctctggcagt aaggatcatt ccggtgtagg gaacacttaa gcggatgctg
    13801 gctattaaaa gtttaaagtc ctcatcggaa accttgtagg gagggttttt tgtaaagtcc
    13861 gttccaatgg caggttcaat acggggaaag gaaatagtgt gaggtcctac tccaaagcgt
    13921 tcttccagat gaattgtatg atagagaaga cccataactt caaaacgcca attatataat
    13981 cccattaaag caccaatgcc caagtcatca attcctgcct gcatagctct atccaaacca
    14041 tataaccgcc aaaggaaatt gcttttaggt cctctgggat gcaaattttt gtaggtctct
    14101 tcataatagg tttcctgaaa aatttgatag gtgccaatac caacggattt tacagtgcga
    14161 aaaccttcaa tatccaaagg agcggcattt atatttactc tgcgaatttc tcctttattg
    14221 tgttttacat tataaacggt ttgcaccgtt tcggcaatga actcgggaga atatttaggg
    14281 tgttcaccgt aaaccataat taatcgttta tgccctttat cttcaagagc ttctgtttcg
    14341 gcaatcaatt cttctttact taaagttgcc cgctgacatt ctttattgga aatacggaat
    14401 ccgcaataaa cacaatcgtt tatacattcg tttccaacat aaagaggagc aaaaagaact
    14461 atccgatttc cataaattcg ttctttgaga gtatgtgcac cttccagaat aagatttttt
    14521 aattcgggat cggtaacatt aattaaagcg gctgtttcat caggattaag acgctgttta
    14581 tccagcgatt tatctacaat atctctaatc ctaatggcat cggcaaaacg gtttttgttt
    14641 aaatgttctt caatttcagc agtaggaata aaggacttaa gaccttcagt attaattttc
    14701 attttgttct ccttcctcca gtttcaaagt tgtaaccttt actttcacgg attttatctg
    14761 ccccagtttt ccggaaaagg cacccatttc ggaaacagag agctctacaa ttaaaaaaat
    14821 tacagcaact cctaaatcgc gcataggata gcccacccgt aaaagaatat tttgtgcata
    14881 actatgcaat aattcgttta cgggatgaaa agcactttca cggtcttcca taataatgct
    14941 tagaatatga tatttggtat tcattttttc cttccttcag cgaggagaaa tttaagatgg
    15001 cagacaaaag aggaaaagag gtcttttcac ccttgatgcc agaacttatc tctcatcgca
    15061 ggtttatttt tcatacatta taatggagat aggatttttg gcaaggaaaa aatgaggttg
    15121 gatgataaaa gtaaaagggg gaaaagtgga aaggtgaaac aaggattgaa catcgtaaaa
    15181 aaataattca ttttgtgtag gcagacgatg cccttcctcc gccagaataa aaatgcagag
    15241 tttcagcaag ctccggctac aaacacgctt tcttcaactg cattatttgg gtttaaatca
    15301 ttaaattata gtaggttacc taaatatatt gtcctttgta atccaaatca gctaattgct
    15361 tattctatag acgattaagt aacgagtccg taacacttcc gtaacacttc cgtagttccg
    15421 ttacggagtt gttacttggt tgttacttgg ttattaaatg gtcaaaaaag caagatagag
    15481 gtaaatttat gccttggaaa aataaagtga gatggaaaaa gaacgacgag gatataattc
    15541 tttcgggttt cttttgtatc cgcttaaaaa ttgaagagat aagcaaaaaa ttgaaaaaat
    15601 gcctataagt agcgaaaaga agtgcagaat tgagtaggaa ttaactcacg ataaaggtca
    15661 aaatgctcta ttttatgtgc gtcgcttccc acagaaaaat atcttcctcc caaatcaagg
    15721 aacatttcta aaagcggaag ttccggaaga aaagaggggt atccccggca taaggaactg
    15781 taattaattt ccagagctat tttgcggtct atcatagtgt taaaaatatc tttcagcaaa
    15841 ggataaaagg cactttcatc aggaatgtaa ttataacccc ttttgtaaac acccaaatga
    15901 gcaagaacat caatattaca atgggaaacc agcaataaat tttgctggta ataatcccta
    15961 acctgttcct cggaaagagg ttttttaagc ggaacagcaa catttgtagc gtcacttaaa
    16021 aaatgaacag aacccaagat aaggtcaaag ttaatatctt caaccaaaga gagggcatat
    16081 tctttaaccc tatgatagtc accaatttca attccacaaa g
//
LOCUS       DAOPFL010000032        16103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_4119_length_16103_cov_84.056082, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000032 DAOPFL010000000
VERSION     DAOPFL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16103
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4119_length_16103_cov_84.056082"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..4071)
                     /locus_tag="PLG69_03825"
     CDS             complement(55..4071)
                     /locus_tag="PLG69_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPI42671.1"
                     /translation="MKKEILFFILLLFISELLGLEVIIGCGTSVTGDSDASPVNVWFK
                     SLHGQSVYTKAELNAAGIFGPVNITQLGFNIVGLPLVSMPNFVVRMKHTTAENVRSWI
                     SADNLVTVYSNPNYLPTETGWNMYNFSIPFEWNGEDNLLVDTAFGVASSYNRSGTVQF
                     TSIGNGYRFKRNDNNDQTNVFSGGETSFQRPNIKLIVEQITIGPQISVNPSSLDFGSI
                     PAGQTQTRQFTIQNNGNETLSGTITTPEGFTVADSLRINLLTADNNAFSRNTLSFTIP
                     AGTSNTYILNFSPLTAGSYNGNVIISSNAENNPLINLQVTGSAYLPPAIEVSTNSLTA
                     TLDAGTQTNQTFTISNTGGLPLSYQISISEDSVYTQKLTQADKSITGSTLTLNYANYE
                     PGATLDWTFSLCNNSTDVEWLKDVYVTFPDGIIVNSATNFTGGTEDMLPNLTSGNGIT
                     IHWHGQTTGGWGVVQSGQTVTAIVNVTILPVFYGDLILSYTIQGDVYGSEPHQIEGQI
                     ALSQILTPIPWFSLEPMQGEVLPGENATITSYFSALTTQPGIYNALLKIVSNDPFQPL
                     TILSVCMNVLQPNHPPVINLPDNLSFNANESLLVDFTPYVSDPDNDPLTLTCSGNTNI
                     NVTITGLQVSLSSLHNWYGTEIIFFNVSDGMFQASDSLTVIVLLNITTGVIVDSDNLP
                     TNWTVDNSPFNIVEPIVIATDQNITLEPGIVVQIWNDEPINVLGSITANNVTFAPGFP
                     DLLWGGLEITGTTGNRTESNITNCEILNAVNAITVNNCSPVINTVYIAPIDTTALING
                     TGITISGISSPPINNITILSYQTCIQVIRSANFLPNEPVINNLMLRNSSVVPRQEEEN
                     TKGIVIEGNSNVIISNAEIKDYNTAIKVENTNVQQSATPSLSNIRIRNTSSTLRNEDK
                     CGIELKGNENAVLYDVRIEECNKGIKIENTETGVSATPSLDFIRIRNTSSTLRQELEN
                     IGILVESNVIPVLRDVQIEDAETGIQINAGGNLDLKYSLLLNCQTGLKNYSTELMPIT
                     RNSFVIEEDQVPPDFLQNCVALSLNDILNFEFSNNTLYGYPKIAAVNSAHLNFINNIV
                     WSNSALNNPFECVNGTYNVTYNDINYGSQVFPGTGNINANPNFVNALELDFALQYNSP
                     CIDAGCPDAEPDPDGTIADMGCYYYHHCAVFESPEGPFYVQQPIQFINNSLGHNTPES
                     YATWLLNGIPVSNDYNLNTIIDTWGNYDLQLVMTSGPLVDSSSVFQFEVIDETPLSPQ
                     NVVLEFSENNYILRWDAVTLSVSQTPITITNYKIYASEDPYGTYTLYQIVPASSRQIN
                     LNLSELGSKRFFKVTAEK"
     gene            4286..5224
                     /locus_tag="PLG69_03830"
     CDS             4286..5224
                     /locus_tag="PLG69_03830"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPI42672.1"
                     /translation="MASTLQFAGRSLFDLDDYSREEIMFILEAAKGMKEINLREYKKI
                     PTLRGKTVCTLFVENSTRTRMSFELAANRLSADVVSFQSSISSLQKGESLQDTVYTLN
                     AMGIDLYCIRHSSPGSPQLVNKYSGKPVINGGDGRHSHPTQALLDIFSIWEKLGDLKG
                     LKITIVGDILNSRVVRSNLIGMGKLGAKVTVCGPKTLMPGNMESVYDCRVEYDLAKAL
                     KDADVVMGLRMQLERMTEGLFPSLEEYSKHYVLSKETLKYAKRNALIMHPGPMNRGVE
                     ILPEIADSNHSIIVEQVANGVAVRMALMFLILGGKA"
     gene            5239..6537
                     /locus_tag="PLG69_03835"
     CDS             5239..6537
                     /locus_tag="PLG69_03835"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPI42673.1"
                     /translation="MKTLIKNANIFQNGKFTPGEILIERKKIIKISKGMASSDYTANK
                     VDKIIDAKGACVFPGFIDLHSHLRDPGQTYKEDIVTGTKAAAHGGFTAVCAMPNTEPI
                     TDNIASVEYIQLRAKDYGSAKVYVIGAITKQSAGEEIAEMATMKAGGIVAVSDDGKCV
                     QNARLMLSCMKYASNFGLPVIIHPEDYFLAGKGQIHSGKVATKLGLSGIPGLAEEVII
                     ARDIMLAESAGVKLHIAHISTARSIELVKNAKDKGLPVTCEVTPHHLVLNEEANLTFN
                     TNTKMKPPLRSEEDRRACVQALQEGIIDCIATDHSPHADFEKEREFDLAPFGIIGLET
                     AFPVLYKNLVQTGQIDLNRLIEALTIAPAKILNLPGGILAEGKTADLTIIDLEKETLF
                     SAENILSKSKNTPWLNQWFPGRVMCTICDGKITYLDSENE"
     gene            6530..7321
                     /locus_tag="PLG69_03840"
     CDS             6530..7321
                     /locus_tag="PLG69_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPI42674.1"
                     /translation="MNKSQPQFLERAIYFKEELSGDYFILWIWDEALGEKCQPGQFFQ
                     IRAHYSIADAEETRSLPKLFKPISIYDNAEGRIGFFIKKVGAGTNALYKLKAGDLLEL
                     IGPSGNGFPLVTGRKILLVSGGIGYPPLWYLQKKLIKHNLLYWVHGGSGQSDVFPCDE
                     VWTEDGSFGKKGMVTEGLGKLIAEKKIDIVYSCGPVPMLKECAIITTELNIEHYCSIE
                     AYMACGIGVCYGCAIPVGQENDWNYLRVCKEGPVFNAREVLWNKF"
     gene            7306..8223
                     /locus_tag="PLG69_03845"
     CDS             7306..8223
                     /locus_tag="PLG69_03845"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPI42675.1"
                     /translation="MEQILNPLQTSLGRLKVNSPVTVASGTFALENLDFIAQEHLGAY
                     VCKTITRYPKKGNPPPRLYETEAGLLNSIGLQNPGLDKFIAEDLPVLRETLTVPLIVS
                     FSGSSLTEFTQMLECLEKQEGISGYEVNVSCPNVENEGIAFGIDPDVVNKLCALLSPL
                     TEREFIVKLSPNVTDIAVIAKAAEEGGATAISLINTIWGMAIDYCNGKSRIKKGIGGY
                     SGIGIKPLALALTYRAAQAVKIPVIAMGGIYNWQDALEFFWAGASMIALGTANFIDPL
                     AVDKVYEGLSSFCLEKQLNLKDIVGKVNK"
     gene            complement(8270..11794)
                     /locus_tag="PLG69_03850"
     CDS             complement(8270..11794)
                     /locus_tag="PLG69_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42676.1"
                     /translation="MKQYIIILILLACSLLTATTLQTFYTNGSILQAHFEGLRQQPVL
                     PEEMDVEDCESANSAVITKTLAFPFEEADLQISAMVWNVFDAEGNYLYNEQKYLENAV
                     SLVQGFTFREMRGYTIRIETQITENNIIRTLKELNFSLIGSYPLQIPAFLSPAFIDAY
                     KALADNFQDSYLRDIPFSRPKMLIITHNQLTNYQQDYISWKKSIGFDVYTVNKSDIGS
                     TVQDIKNFIANHYQTYKCDYLFLWGDVNGTYAIPTNFIVSPEYATENDADDNYYTMLE
                     GDDYFPEMIAGRFSFADASEFITMMNKTIAYEKTPFMTDTNWMKRALAVAGNYADGGL
                     RPTTPISMSQWLRDKMRNFGYTQVDTVFCPPTYPGTTTIQQSINQGVQFISYRGWGDA
                     NGWHFPSFHIPDLNNTFNGAKMPIVYSIVCNTGDFANAVNPNFGEKWMRMGTMSNPGG
                     CVAFVGPSDLHTKTRFNNSIASGAFRSIMDWDVRCFGSSVLMGKIELYKNFPNDLAPN
                     QYVAFYFHVFNILSDPSLNMWVLVPNQIPETVIASGLTFQQSDSHIRINAPNLNSGIV
                     SGTKDGINFSYAPIENGYAILPIDPNVEGNLTITVSKKNYVPLVKQLTLQPDATIGIV
                     SNNLANTILAPNESYSLGLQLKNFSSQAYNNITVNLACNHPAIVIDNPNATITSLNPE
                     ATVSVNFLFITTGAIRPGAIIDFTVTLNNPSVTCLFQLVAGGAKIQVINYEGVLEIGQ
                     ANNITFTVTNLGNFTMEDIVLQLQALTPAAVAQNNPIALGSLAPNEVKQFTATITLMN
                     DVFPGNNIPLRFDATNAEGYSYFSFYAITAGTPGTDDPTGPDEYGYFAYDSTDLGYPS
                     TPIYDWIELDPEGGNLLGNVFLCKDDSVKTVPLPFTFRFYGVDYNGVTMSSNGWISFI
                     PTDQSDFYNCYIPAALGPYAMVAGYWDDLKGMKTGEDESGNPIFANMRIIYWYDSTNN
                     RYIIEWNKAYNQYTIDLGPNASMEKFQIILYPKLEQDGDIVIQYHTVDNPGITTNYCT
                     VGIEDHNQLRGLTYTHSNTYPVTATPLTPGLAIKFTTTWPDNYVANEDETLPVPVCNL
                     HNYPNPFNPVTTISFTAKQKGFATLNIYNLKGQLIKTLLKDNILSGEHKLIWDGKDNQ
                     GNNVASGVYLYKLNMNGSSQIKKMLLLK"
     gene            11969..12877
                     /locus_tag="PLG69_03855"
     CDS             11969..12877
                     /locus_tag="PLG69_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPI42677.1"
                     /translation="MKNGKTIIIVIIALLALVSCGKNTVLNPDANDPTAINFGTSNTL
                     DVVTWNLRTFPEGTDLTTLKEMISALNAEVIAFQEIMDYNAFMDLAEEIPYYDACVYS
                     ATDTYRLAYLYDTRSVTVNNQYTIFENDSNPFPRPPYILDITWNNSNYFIINNHFKAY
                     GDNYIDESDSWDEEMRRRLACQKLDNYISTSLSDKKVIVLGDMNDQIAEPEDYNVFLS
                     FLNKPEEYYFADMPIALSPTYSNVSYPSSLSHIDHILITNELFADFENAGSYCKTILA
                     ENWMLSWNTYSSTISDHRPVGIRISK"
     gene            complement(13128..14081)
                     /locus_tag="PLG69_03860"
     CDS             complement(13128..14081)
                     /locus_tag="PLG69_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPI42678.1"
                     /translation="MPKNTKIQHLQREVGRLFGIVFASFFFAMGYSWFLLPYNMAPGG
                     VGGISQILYHFLGIPNGVSMIILNIPLFIISFIFIGKSFGSKSLYGMFVSSIMTDLLS
                     FPALHKMGIISDLKPYTHILNGHKIYAMLAPEDILLSAIAGSVLLGLGLGLIFRFRGS
                     TGGTDIPVALIKQKANLSIGTGYFIVETGIILLVAITFKAPKLLIWGYVNLFITTKIT
                     DLASEGLPYIKGAYIISDDVEEIRLQIFTKLERGVTYLNGVSGFHQRDIKILFVVLNR
                     RQVPLLTDIVRDVDPDAFMIIMDVYDVLGYGFKSRSLNLSE"
     gene            14191..15852
                     /locus_tag="PLG69_03865"
     CDS             14191..15852
                     /locus_tag="PLG69_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPI42679.1"
                     /translation="MILDIRINGIKHTLLKLDRPCSLADIMKRTNINQSIILAIKINH
                     REYVNEDYIPDRETLIDFITYLHPEGYRIYQDSVIFIMAKALHTLMGTAHSLVVEHSI
                     ADGVFCEVFNTQNFTEADCSCLKTAMQNIVDSDLPIEKIMVKTEEALDIFSAMGRNDV
                     IKNLKYHYQENVAIYRCGKYYDCFVHPLVERTGKVKTFDIVYRSPGFILRFPSRDEKM
                     EIKPFELPHKLFAQHQEHDKWLDILRVHNIIDINKLIDNYEISEFIQVEEALHEKKIA
                     EIAADIVTKQDVKFILIAGPSSSGKTTFAKRLGVQLQACKAKPVVIGMDDYFLPRDKT
                     PRKPDGEYDFESIYAMDLEYLNMQMNQLLEGEQIELPRYDFTRGVRRHSNNFVKLAPN
                     SIIIMEGIHGLNETLTSSIAASRKVKIYVSALNQLNIDNHNRIATTDCRLLRRIIRDH
                     RYRGYSAEETLIRWQDVREGEDKNIFPYQENADYMFNSSLTYEIGVIRKHAWKLLLGV
                     SPSSSAYMEAKRLSGLIANCKDIADSLVPYNSIIREFTDGSIFRY"
     gene            15862..>16103
                     /locus_tag="PLG69_03870"
     CDS             15862..>16103
                     /locus_tag="PLG69_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42680.1"
                     /translation="MSNIHILAEDVRNKIAAGEVIERPASVVKELVENAIDAGADSII
                     VAIENGGKDLIRVIDNGCGMNADDAMLAFERHSTSK"
BASE COUNT         5184 a         2917 c         3210 g         4792 t
ORIGIN      
        1 tctcaacctc cttaaccctc tcaaccttat tttccgttta ctacataata ttttttactt
       61 ttcggcagtt accttgaaaa agcgtttaga gccaagttca gacaggttca ggttaatttg
      121 tctgctacta gcaggcacta tttgatatag ggtataagtt ccgtaggggt cttcactggc
      181 gtaaattttg taattagtaa tcgtaatagg agtttgggat acggaaagag taacagcatc
      241 ccaccgtaaa atatagttgt tttccgagaa ctctaaaaca acattttgcg gagatagtgg
      301 tgtttcatct atcacctcaa attgaaaaac actgctgcta tccacaaggg gtccggaagt
      361 cattactagc tgcaaatcat agttacccca ggtatcaata atcgtgttca gattatagtc
      421 gttggaaaca ggtattccat tcaaaagcca ggtagcataa ctttcgggag tgttatgccc
      481 aagggaatta ttgataaact gaataggttg ctgaacatag aaaggacctt ccggcgattc
      541 aaaaacggca caatgatgat aatagtagca tcccatatcg gcaatagttc catcagggtc
      601 tggttccgca tcaggacacc cggcatcaat acagggactg ttatattgca aagcgaaatc
      661 aagctccaaa gcattcacaa aatttggatt agcattgata ttaccagttc cgggaaagac
      721 ctgactgcca tagttaatat cgttgtaagt aacattataa gttccgttta cgcattcaaa
      781 gggattattc agggcagaat tgctccagac aatattattg ataaaattca gatgggcaga
      841 attaacagca gcaattttag gataaccgta gagggtatta ttgctaaatt caaaattaag
      901 tatatcatta agagagagag ccacacaatt ctgtaaaaaa tcaggtggca cttggtcttc
      961 ttcaataaca aagctattac gggttatagg catcagttcc gtactatagt ttttcagccc
     1021 tgtttggcaa ttcagaagaa gggaatattt cagatccaga tttcctccgg cgttaatctg
     1081 tatacctgtt tccgcatctt caatttgaac atcgcgcaaa acaggtatta cattactctc
     1141 aaccaaaatt ccgatattct ccaattcctg ccttaaagtg ctgctggtat tgcggatacg
     1201 aataaaatcc aaggaaggag ttgccgacac tcccgtttca gtattttcta ttttaattcc
     1261 tttattgcat tcctcaattc tgacatcata taaaacagca ttttcattgc cctttagttc
     1321 aattccgcat ttatcttcat ttcgtaaagt actacttgta ttacgaatgc ggatattgct
     1381 tagggaagga gttgcggact gctgaacatt ggtattttcc accttgatag ctgtattata
     1441 gtcctttatt tcggcatttg aaataattac attgctatta ccctcaatta ctataccctt
     1501 cgtattttcc tcttcctggc gaggcacaac agaagaattt cgtaacatta gattattgat
     1561 aaccggttca ttgggaagga aattggcact gcgtataacc tggatacagg tttgatagct
     1621 taagatggta atattattta tgggaggact agaaataccc gaaatagtaa ttcctgtgcc
     1681 attaattaaa gcagtggtat ctatgggggc tatatagaca gtgttaataa caggactaca
     1741 attatttacg gtaatagcat tgacagcatt caggatttca cagttggtaa tattgctttc
     1801 tgttcggttt cctgtcgttc ccgtaatttc caaaccaccc caaagcaaat ccggaaagcc
     1861 aggtgcaaaa gtgacattat tggcagtgat agaacccaga acattgatag gttcatcatt
     1921 ccatatttga acaacaatcc ctggttccag agttatattc tgatcggtag caattactat
     1981 tggctccaca atattaaaag gtgaattatc aaccgtccag ttagtcggta aattgtccga
     2041 atccacaatt actccggtag taatattcag caaaacaatt acggttaaag agtcgctggc
     2101 ttgaaacata ccatcagaaa cattaaaaaa tataatttca gttccatacc agttatgcaa
     2161 agagctcaaa ctaacctgca aaccggtgat ggtaacattg atattggtat ttccactaca
     2221 agttaaggtt aagggatcgt tatctggatc gcttacataa ggagtaaaat ccactaataa
     2281 tgattcattg gcattgaaag agagattatc cggtaaattt atcaccggag gatgattggg
     2341 ctgaagaaca ttcatacaaa cagataggat tgtaagtggt tgaaaaggat cattggaaac
     2401 gatttttaag agggcattat atattccggg ttgggtagtt aaggcagaaa aatacgaggt
     2461 tatggtggca ttttcacctg gaagaacttc cccctgcatt ggctcaagag aaaaccaggg
     2521 aatgggagta aggatttgac taagcgcaat ttgaccctct atttgatgtg gctcactgcc
     2581 ataaacatca ccctgaatag tataagacag gataaggtct ccataaaaga caggaagaat
     2641 cgttacattt acaattgctg tgacagtttg tccactctga acaactcccc agccaccggt
     2701 tgtttgtccg tgccagtgaa ttgtgattcc atttccggaa gttaaattag gaagcatatc
     2761 ttcagttccg cctgtaaaat tggtagcgga attgacaata attccatcgg ggaaagttac
     2821 atacacatct ttcagccatt caacatcagt gctgttatta caaagagaga aggtccaatc
     2881 caaagttgca cccggttcat aattagcgta gttcaatgtt aaggtgctgc ctgtaatact
     2941 tttatcagct tgggtaagct tttgagtata aacgctatct tcgcttattg agatttgata
     3001 agataagggc aaaccacccg tattggaaat agtgaaggtt tgatttgtct gggtgcctgc
     3061 atccaaagtt gctgtcaatg aattggtact cacttctatt gctggaggaa gatatgcact
     3121 gccggtaact tgtaaattaa taagaggatt gttttcagcg ttactggata taattacatt
     3181 tccattataa cttcctgccg taaggggaga aaaatttaaa atataagtat tggatgtacc
     3241 ggcagggata gtaaaactaa gtgtattgcg gctaaaagca ttgttatccg ctgtcagtaa
     3301 atttatcctt aagctatccg caacggtaaa accctcggga gttgtgattg ttccgcttaa
     3361 ggtttcatta ccattatttt ggatagtaaa ttgcctggtt tgggtttgtc ctgcaggaat
     3421 actgccaaaa tcaagagaac ttgggtttac tgaaatttga ggaccgatag taatttgttc
     3481 tacaataagc ttaatattgg ggcgttgaaa agatgtttct cctcctgaaa aaacattggt
     3541 ctgatcgtta ttatcatttc ttttgaaacg ataaccattt cctattgaag taaattgaac
     3601 agttcctgaa cgattataac tgcttgccac tccaaaagca gtatctacta aaaggttatc
     3661 ctctccattc cattcaaaag gaatggaaaa attatacata ttccaaccgg tttcagtagg
     3721 taaataattg ggattagaat aaaccgttac caaattatct gcactaatcc atgatctgac
     3781 attttctgca gttgtatgtt tcatacggac gacgaaatta ggcattgaaa ctaagggtaa
     3841 accaactata ttaaaaccaa gctgggtaat atttacagga ccgaagatac cagcagcatt
     3901 taattctgct ttagtataaa cggattgacc gtgtaaactt ttaaaccata cattaacagg
     3961 tgatgcatca gaatctcccg ttacagaagt tccacagccg attataactt ctaaaccaag
     4021 taattctgaa ataaatagga gtaggataaa gaataaaatt tcttttttca tattaacaac
     4081 tccgttcttt aaaaaaatta attctttaat ttaaaacctt cctcttcggc aaatacgaat
     4141 taactattat tcccagagga cgatcttaga ataaatcacc attacaatat aaaatataat
     4201 caaaaaattc acttgaccaa attgggattt ctaataaaag ggaaaaaacc tttaatcaca
     4261 gccagtgagc tggaaaggag taaaaatggc ctcaacacta caatttgcag gacgctctct
     4321 gtttgacctg gatgattatt cccgggaaga aattatgttc attctggaag cagccaaggg
     4381 aatgaaagaa atcaacttac gggaatacaa aaagattcct accttaaggg gaaaaacagt
     4441 gtgcacttta ttcgttgaaa acagcacccg cacgcgaatg agttttgaat tggctgccaa
     4501 tcgtttaagt gcagatgttg ttagttttca gtcctccatt tcctctctgc agaaaggaga
     4561 aagtttacag gatacggttt acactttaaa tgcaatgggt atagaccttt actgcattcg
     4621 tcacagcagt cccggaagtc cgcaactggt aaataaatat tccggtaaac cagttattaa
     4681 tggaggagat ggaagacact cacatcccac ccaggcacta ctggatattt tttctatctg
     4741 ggaaaaatta ggtgacctga aagggctgaa aataaccatt gtaggcgaca ttttaaatag
     4801 tcgcgtagtg cgtagtaatt taattggaat gggaaaactg ggtgccaaag taactgtttg
     4861 cggacctaaa actttgatgc caggaaatat ggaaagtgtt tatgactgcc gtgtagaata
     4921 tgatttagca aaagctttaa aagatgccga tgttgtaatg ggattaagaa tgcagttgga
     4981 acggatgacg gaagggcttt tcccctctct ggaagaatac agcaaacact atgttctctc
     5041 caaagaaacc ttgaaatatg ccaagaggaa tgctttaatt atgcatcccg gtcctatgaa
     5101 cagaggagtg gaaattctgc cggaaattgc agatagcaat catagtatca ttgtggaaca
     5161 agttgccaat ggagttgcgg ttagaatggc tttgatgttt ttaatcttag gtggaaaagc
     5221 ataaggagga aaaaaacaat gaagacctta attaaaaatg ccaatatctt tcaaaatggg
     5281 aagttcaccc caggggaaat cttaattgag agaaagaaga ttataaaaat aagcaaagga
     5341 atggcgtctt ctgactacac agcaaacaaa gtggataaaa ttatagatgc caaaggtgct
     5401 tgtgtgtttc ctggttttat agacctgcat tctcatttac gcgacccggg acaaacctat
     5461 aaagaagata ttgtaacagg aactaaagct gccgcgcatg gtggatttac agctgtttgc
     5521 gcaatgccaa atactgaacc tattaccgat aatattgctt ctgtagaata tatccaatta
     5581 cgggctaaag attatggctc tgccaaggta tatgtaattg gagctattac gaaacagtca
     5641 gctggtgaag aaattgctga aatggcaaca atgaaagcag gaggtattgt agctgtttcc
     5701 gatgacggta aatgtgtgca aaatgcacga ttaatgcttt cctgtatgaa atatgccagt
     5761 aactttggtc tgccggtaat tattcatcct gaggattatt tcttagccgg aaagggacag
     5821 atacattccg gaaaagttgc taccaaactt ggacttagcg gaattcccgg tctggcagaa
     5881 gaagtgataa ttgcccggga tattatgctc gctgaaagtg ctggagttaa attacatatt
     5941 gcccatattt ctacggcgcg ctctatagaa ctggtaaaaa atgccaaaga taaaggactt
     6001 cctgttacct gtgaagtaac gcctcatcat ttggtgctga atgaagaggc aaatttaact
     6061 tttaatacta atactaaaat gaaacctcct ctgcggagtg aagaagaccg tcgggcttgt
     6121 gttcaggcat tgcaagaagg aataattgat tgcattgcta cagaccattc tccccatgcg
     6181 gattttgaaa aagaaaggga atttgacttg gctccctttg gcataattgg cttggaaaca
     6241 gcatttcctg tgctctataa aaacttggtg cagacagggc agatagacct caaccgttta
     6301 attgaggcat taacaattgc tccagccaaa atcctaaatt tgcctggagg aattttggcg
     6361 gaaggaaaaa cagccgattt aactattata gacctggaaa aggaaactct tttttccgcg
     6421 gaaaatatac tttccaaaag caaaaatact ccctggctca atcaatggtt tcccggaagg
     6481 gtgatgtgta ctatctgtga tggcaaaata acttatctgg atagtgaaaa tgaataaatc
     6541 ccagccacaa tttctggaaa gagcaatata ctttaaggaa gaactctccg gggactattt
     6601 tatcctgtgg atttgggatg aggcattggg ggaaaaatgc cagccgggac agttcttcca
     6661 aatcagagct cactatagta ttgccgatgc ggaagaaaca cgctctctgc ctaaactctt
     6721 taaacctatt tccatttatg acaatgctga agggagaatt ggtttcttta ttaaaaaagt
     6781 gggtgccgga acaaatgctc tctataaact gaaagccggt gaccttttag agcttatagg
     6841 tccttcggga aacggttttc ctttagttac ggggagaaaa atattactgg ttagtggagg
     6901 tattggctat cctcctttgt ggtatttgca aaagaaatta attaaacata atttgcttta
     6961 ttgggtgcat ggcggttctg gacaaagtga tgtttttcct tgtgatgagg tctggacaga
     7021 agatggcagt tttggtaaaa aaggaatggt tacagaagga ctgggaaaat tgattgcaga
     7081 aaagaagatt gatatagtat atagctgcgg tcctgttcca atgctgaagg aatgtgcaat
     7141 tataactacc gaactgaata ttgaacatta ttgttcaata gaagcatata tggcttgcgg
     7201 gataggtgtt tgctacggtt gtgccattcc tgttggtcaa gaaaacgact ggaattattt
     7261 acgggtctgc aaagaaggtc ccgtttttaa tgccagggag gtattatgga acaaattcta
     7321 aatccgctgc agacctcttt gggacggctt aaagtgaata gtcctgttac tgttgcttcc
     7381 ggaactttcg ctttggaaaa cctggatttt attgctcagg aacatttagg ggcttatgtt
     7441 tgtaaaacta ttacccgcta tccgaaaaag ggaaatccgc ctccgcgttt atatgaaact
     7501 gaagcaggac ttctgaattc catcggttta caaaatcctg ggttggataa gtttattgct
     7561 gaggacttgc ctgttttgcg tgaaactctt actgttccat taattgtaag tttttccggc
     7621 tcttcgttaa ctgaatttac tcaaatgctg gaatgtttgg aaaaacaaga gggaatttct
     7681 ggctacgaag tgaatgtatc ctgtcccaat gttgaaaatg aaggtatagc tttcggaatt
     7741 gatcccgatg ttgtaaataa actatgtgca cttctttcac ctttaacgga aagagaattt
     7801 atagtaaaat taagtcccaa tgttacggat attgctgtta ttgctaaagc tgctgaagaa
     7861 gggggtgcta ctgcaatatc attaatcaat actatttggg gaatggcaat tgactactgt
     7921 aatggaaaat caaggattaa aaaaggaatc ggtggctata gcggaatcgg aattaaaccc
     7981 ttagccttgg ctttaactta tcgtgctgct caagctgtta aaattcctgt tattgctatg
     8041 gggggaattt ataattggca ggatgctttg gagttctttt gggctggggc atctatgatt
     8101 gctttgggaa cagcaaattt tatagaccct ttagctgtgg ataaggttta tgagggatta
     8161 tcctcttttt gtctggaaaa acaacttaac ctgaaagata tagttgggaa agtaaataag
     8221 tgaacagtga agggtgaatt cacctttcac tgttcacccg ttcgttcatc tatttcagta
     8281 gcagcatctt tttgatttgg gaactgccat tcatattcag tttatagaga taaaccccgg
     8341 aggcaacatt atttccctga ttatcttttc cgtcccaaat cagtttatgt tcaccggata
     8401 aaatattatc ttttagcagg gttttaatta gctgtccttt caagttataa atattgaggg
     8461 tagcaaaccc tttttgtttc gcggtaaagg aaatcgttgt aaccggatta aaaggattgg
     8521 gatagttatg taagttacat acgggaacag gcaaggtctc atcttcattg gcaacataat
     8581 tatcgggcca ggtagtagtg aatttaatag ccaaacctgg agttaaagga gttgccgtta
     8641 caggataagt attactatgg gtgtaagtta aaccgcgtaa ttgattatgg tcttcaattc
     8701 ctacagtgca atagttggta gttattccag gattatcaac ggtatgatat tggattacga
     8761 tatcgccgtc ttgttccagt ttcggataga ggataatctg gaatttttcc atagaagcat
     8821 tgggacccaa atctatagta tattgattgt atgctttgtt ccattcaatg atataacgat
     8881 tattagtgga atcataccaa tagataattc gcatattagc aaaaatagga ttacccgatt
     8941 catcctcacc tgttttcatc cctttaagat catcccaata accggcaacc atagcataag
     9001 gacctaaagc agcaggaatg taacaattat agaaatcgga ttgatccgta ggaataaaag
     9061 aaatccaacc gttggaagac attgtaacgc cattataatc aacaccgtaa aaacggaaag
     9121 tgaaaggcag aggaaccgtt ttcacagaat catccttaca taagaagaca ttacccaata
     9181 aattgcctcc ttcaggatca agctcaatcc agtcataaat gggagttgaa ggataaccga
     9241 gatcagtaga atcataagca aaataaccat attcatcagg tcctgtagga tcatcagttc
     9301 caggagtgcc agccgtaatt gcataaaaac taaagtaact gtaaccctct gcattagtgg
     9361 catcaaaacg cagagggata ttatttccgg ggaagacatc attcatcaaa gttattgttg
     9421 ctgtaaactg tttaacttca ttgggagcca gagaaccaag agcaataggg ttgttttgag
     9481 caacggcagc aggtgtaagt gcttgtaatt gtaaaacaat atcttccata gtaaaattgc
     9541 ccagattggt aaccgtgaaa gtaatattat ttgcctgtcc tatttctaaa acaccttcat
     9601 aattgatcac ctgaatttta gctccaccag caactaactg aaacaaacaa gtaacggaag
     9661 gattattcaa agtaacagta aagtcaatta ttgcaccggg tctaatagct cctgtggtaa
     9721 taaaaaggaa atttacagac acagttgctt ccggattcag agaagtgata gtagcattag
     9781 gattatcaat cacaatagct ggatgattac aggcaagatt aacagttata ttattatacg
     9841 cctggctgga gaaattttta agctgtaagc ccagagaata tgattcattc ggagccagaa
     9901 tagtattagc taaattatta gaaacaatac cgatagtggc atccggttgc aaagttagtt
     9961 gcttcaccaa aggaacataa ttctttttgc tgacggttat ggttaaattg ccttccacat
    10021 tagggtctat aggtaaaata gcataaccat tttcaatagg agcataactg aagttgatac
    10081 cgtctttggt tccggatact ataccgctat tcaagttagg ggcatttatt ctgatatgac
    10141 tgtcactttg ctgaaaagta agtccactgg caattacggt ttctggaatc tgattgggca
    10201 ctaaaaccca catatttaaa ctgggatcac ttaaaatgtt aaacacatga aagtaaaaag
    10261 caacatactg attgggggca aggtcattag ggaagttctt gtagagttca attttaccca
    10321 tcaaaacact ggaaccgaaa caacgcacat cccaatccat aatactgcga aaagctccac
    10381 tggcaataga attattaaat cgggttttag tatgtaaatc ggaaggaccc acaaaagcaa
    10441 cacaacctcc cggattggac attgttccca tacgcatcca tttttcaccg aaatttgggt
    10501 ttacagcatt ggcaaaatca cctgtattac aaacaatgga atacacaatc ggcattttag
    10561 caccgttaaa agtattgttc aaatcgggaa tgtgaaaaga aggaaaatgc caaccgttag
    10621 catcacccca accacgataa ctgataaatt ggacaccttg attgatggac tgttgaatag
    10681 tagttgttcc aggataagtt ggaggacaaa aaacagtatc aacttgagta tagccaaaat
    10741 tacgcatttt atctctcaac cattgagaca tagagatagg agtagtaggt cttaaacccc
    10801 catcagcata atttccggca acagcgagag ctcttttcat ccaattagta tctgtcataa
    10861 aaggggtttt ttcgtaagca atggttttat tcatcattgt aataaattcg ctggcatcgg
    10921 caaaggaaaa tctgcccgca atcatttccg gaaagtaatc atctccttcc agcattgtgt
    10981 aataattatc atccgcatcg ttttctgtag catattcagg agaaactata aaattcgtag
    11041 gaatggcata agttccattc acatcacccc acaggaaaag atagtcgcat ttataggtct
    11101 gataatgatt ggctatgaaa ttcttgatgt cctgaacggt ggaaccgata tctgatttat
    11161 ttaccgtata aacatcaaaa ccgattgatt ttttccagga aatatagtcc tgttggtagt
    11221 ttgttaattg gttgtgagta ataatcagca ttttaggacg gctgaagggt atatcccgca
    11281 aataggaatc ttgaaaatta tctgccaaag ctttgtaggc atcaataaaa gcgggggaaa
    11341 gaaaggcagg aatttgaagc ggataagaac cgattaagga aaaattgagt tcctttaaag
    11401 tccggattat attgttttcc gttatttgag tttctatccg aatcgtataa ccgcgcattt
    11461 ctctaaaagt gaaaccctgc acaagagaaa cagcgttttc taaatacttc tgttcattat
    11521 ataaataatt gccttccgca tcaaaaacat tccagaccat agccgaaatt tgcaaatccg
    11581 cttcttcaaa aggaaaggca agagttttag tgataacagc gctattagca gattcacaat
    11641 cttcaacatc catttcttca ggcaaaaccg gctgttgtct caagccctca aaatgtgcct
    11701 gtaaaataga accattagta taaaaggtct gcagtgttgt agctgttaac aaactgcagg
    11761 caagcagaat caggattatt atatattgct tcatttatcc tccacctata ttgtgtagtt
    11821 aatgtataat gcaataaccc ttccaacctc ggtaataatt tttgctctca aatattttac
    11881 ggaaattggc aaaagaattg cattattata tactccttcc gaagttgggc ttgaaaacct
    11941 agatatttgg aagtttaagg aacaataaat gaagaacgga aaaacgataa taattgtaat
    12001 aatagcgttg ttggcgttgg tctcctgcgg gaaaaacaca gttctgaatc ctgatgctaa
    12061 tgatcctaca gcaatcaatt tcggaacttc aaacacgctg gatgtagtta cctggaattt
    12121 aagaaccttt ccggaaggga cggatttaac tactcttaaa gaaatgattt ccgctttgaa
    12181 tgcggaagtt atagcatttc aggaaattat ggattataat gcctttatgg atttggcgga
    12241 agaaattccc tattatgacg cttgtgttta ttctgccacg gatacttatc gtcttgccta
    12301 tttgtatgat acaaggtcag ttaccgttaa taatcaatac actatttttg agaatgacag
    12361 caatcctttt ccgcgtccac catacatttt ggatataacc tggaataaca gtaactattt
    12421 tattataaac aatcacttta aagcatacgg agataattat attgacgaaa gtgacagctg
    12481 ggacgaagaa atgcgtcgtc gtcttgcctg tcagaaactg gataactata tttctacctc
    12541 tctatcggat aaaaaagtga ttgttttggg tgatatgaac gatcagattg ccgaaccgga
    12601 ggactataat gttttcctta gttttctgaa taaaccggag gaatattact ttgccgatat
    12661 gccgattgct ctttctccaa cctattccaa tgtttcttat ccaagttcac tcagccatat
    12721 agaccatatt ttgattacca atgaactttt tgcggatttt gaaaatgcag gaagttattg
    12781 taaaacgatt ttggcagaga actggatgct gagctggaat acctattctt ctacaatttc
    12841 agatcataga cccgtgggga ttcgtatatc caaatagcaa tatagattaa acggatttta
    12901 cggattaact cttccagata atatagatta actctcacag ataacactga taacacagat
    12961 ttatatttta caattctttc ctaaagacag caaatgttat cccttcttct ttattaatag
    13021 cttcattttt cattctcttt tgccaagtag cattgcttac tcattgctta cccattactg
    13081 atggcattag caatgggtaa gcaatgagta tgcaatacaa ttaaaaatca ctcactcaaa
    13141 tttaaactgc gtgacttaaa gccatatccc agaacatcat acacatccat aataatcata
    13201 aaagcatcag gatcaacatc tcttactata tctgtaagca aaggcacttg acgacggttc
    13261 aacaccacaa aaagtatttt tatatctctc tgatgaaaac cgctaactcc attgagatag
    13321 gtaactccgc gttccagttt agtaaatatt tgcaggcgaa tttcctctac atcatcggaa
    13381 atgatataag cacctttaat ataaggtaaa ccttcacttg ctaaatcggt tattttggtt
    13441 gtaataaata gatttacata accccagata agaagttttg gtgctttaaa ggtaatagct
    13501 accagaagga taattcctgt ttccacaata aagtatcccg ttccaataga aaggtttgct
    13561 ttttgtttaa tgagtgctac aggaatatct gttccccctg tagaaccccg aaaacgaaaa
    13621 atcagtccta aacccaatcc caataataca ctaccggcaa ttgcacttaa aagtatatct
    13681 tccggagcaa gcatagcgta gattttatga ccgttaagaa tatgtgtata gggctttaaa
    13741 tcggaaataa ttcccatttt atgtaaagca ggaaaactta aaagatcggt cataattgaa
    13801 cttacaaaca taccatacaa gcttttgcta ccgaaggatt tgccgataaa gataaaacta
    13861 atgataaaca atgggatatt caaaataatc attgaaacac cattgggaat tcccaaaaag
    13921 tgatataaaa tctgtgaaat acctcctacc cctccaggag ccatattata gggtaataaa
    13981 aaccaggaat aacccatagc gaaaaaaaag gaagcaaaga cgattccgaa caagcgtcct
    14041 acttctcttt gcagatgctg tattttagta ttctttggca ttttctatct ctcctaaatt
    14101 cttgactaaa tcttaatcat taaatctttg gtttacgaaa aaataacatt atacatcccc
    14161 attatttgag ggaacaacag gaaacaaaag atgattttag acattcgcat aaacggaatc
    14221 aaacacactc tgctgaaact ggatagaccc tgttcactgg cagatataat gaaaagaacg
    14281 aatataaatc aatccattat attagccatt aaaattaatc atcgggaata tgtcaatgaa
    14341 gattacattc cagacaggga aacattgata gattttatta cctaccttca tcccgagggc
    14401 tatagaattt atcaggatag cgttattttt attatggcta aggcattgca tactttgatg
    14461 ggaacagcgc actctctggt agttgaacat tccattgccg atggtgtttt ctgtgaagtt
    14521 ttcaacacgc aaaattttac ggaagcggat tgcagttgcc tcaaaacagc aatgcagaat
    14581 attgtggata gcgatttgcc gattgaaaaa attatggtta aaactgaaga agcattggat
    14641 atctttagtg ctatgggtcg caatgatgtg attaaaaatt tgaaatatca ctatcaggaa
    14701 aatgtggcaa tttatcgttg cggaaaatat tacgattgtt tcgttcatcc tttggtggaa
    14761 agaacaggaa aagtaaagac ctttgatatt gtttatcgct ctcctggctt tattttacgc
    14821 tttcccagcc gggatgaaaa gatggaaata aaaccctttg aacttccgca taaactcttt
    14881 gcccagcatc aggaacacga taaatggctg gatattttac gcgtccataa cattatagat
    14941 atcaataaat tgattgataa ctacgaaatt tcggaattta ttcaggtgga agaagcatta
    15001 cacgagaaaa aaatagccga aattgctgcc gatatagtta ccaaacagga tgtgaaattt
    15061 attttgatag cgggtccttc ttcttccggc aaaaccactt ttgccaaaag attaggagtt
    15121 cagcttcagg catgtaaagc caagccggta gttattggaa tggatgatta ctttttgccc
    15181 cgggataaaa ctcctcgcaa acccgatggt gaatacgatt ttgagtccat ttatgctatg
    15241 gacctggaat atttgaatat gcaaatgaac caattgctgg aaggcgaaca gattgaattg
    15301 ccccgttatg attttaccag aggtgtgcgt cgtcacagca ataattttgt aaaacttgcc
    15361 cctaacagta taattattat ggaaggtatt cacggtttga atgaaacatt aacttcttcc
    15421 attgctgcca gcaggaaagt gaaaatttat gttagcgctc ttaatcaatt gaatatagat
    15481 aatcataacc gaattgctac aaccgattgc agattgctgc gcagaattat tcgcgaccat
    15541 cgttatagag gttatagcgc tgaagaaaca ttaatacgct ggcaagatgt gcgcgaaggt
    15601 gaagataaaa acatttttcc ctatcaggaa aatgcggatt atatgtttaa tagcagttta
    15661 acttacgaga ttggagtaat tcgtaaacat gcctggaaat tgcttttagg cgtttctcct
    15721 tcctcttctg cctatatgga agctaagcgt ctttccggtt taattgccaa ttgtaaagat
    15781 attgccgatt ccctggtgcc ctataattct attatcagag aatttacgga cggcagtatc
    15841 ttccgttatt gaggttttaa agtgtctaat atccatattt tggcagaaga tgtccgtaat
    15901 aaaattgctg caggagaagt aattgaacgc cctgcttcag tggttaaaga actggtagaa
    15961 aatgccattg atgccggtgc tgatagtata attgtagcta tagaaaacgg tggtaaagac
    16021 ctgatcaggg ttatagataa cggttgtgga atgaatgctg atgatgcaat gctggctttt
    16081 gaacgccatt ctaccagcaa aat
//
LOCUS       DAOPFL010000033        15933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_4184_length_15933_cov_90.452513, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000033 DAOPFL010000000
VERSION     DAOPFL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4184_length_15933_cov_90.452513"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(412..1044)
                     /locus_tag="PLG69_03875"
     CDS             complement(412..1044)
                     /locus_tag="PLG69_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPI42681.1"
                     /translation="MNKNKFSSIIVLPDCVIKYYSSYKHFQAELFAYQLNLPMLPKLL
                     DYAEPYWIKMEKITGIPYLDELNNFQPALLAETIANFHWATLQNDKCLCHKDNAPKNI
                     LFCQGKYYFIDFTESEFAYPEKDITHLLLFWAADFPGEFFRSSVTLFLNTYLSILQLN
                     SERWMTSLQENICLFDNRRKNFGKHSGKQPIAIQSANRKWLAEFEVHINS"
     gene            complement(1037..1852)
                     /locus_tag="PLG69_03880"
     CDS             complement(1037..1852)
                     /locus_tag="PLG69_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPI42682.1"
                     /translation="MKAKTLYLILILLVLVAAIITLILLSRKDPLNGPESIAFDAVGN
                     RFLISNTLGKSIVALSLDEQYSPFLDKGLEEPRGIIIHSGKLYVADKTAIKIVDPEKA
                     VIIDTLKIPGAVMLNDLAFTKTGIIYTTDTGGDCVYVIDPIANKMQKLTSPLLKKPNG
                     IVYDMPRDQMFVVCFKEKSPILSISTLDNRISIFKDTIYDYLDGIAIDDLGRIYITSW
                     GQDMIFEIPQEQNRYLPTFKGIKDAADIFYYLPANQLIVPLFSDNQIIRLQLE"
     gene            complement(1849..3258)
                     /gene="cysS"
                     /locus_tag="PLG69_03885"
     CDS             complement(1849..3258)
                     /gene="cysS"
                     /locus_tag="PLG69_03885"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPI42683.1"
                     /translation="MLLYNTQTRKKEEFQPITPGKVGIYACGPTVYNYFHIGNARAFI
                     FFDVVRRYFAYRGYEVTYVQNITDIDDKIIAQANEENISFTLVAEKYTRAFLEDTSAL
                     GIKPPTYQPKATEVMDDIIAFIAELEKKGYAYEVEGDVYFATENLPSYGCLSGKKTEE
                     QLAGARVMENYNKRHPADFTLWKKGKPGEPVWESPWGKGRPGWHTECVVMSQKYLGET
                     FDIHCGGIDLIFPHHENELAQAMAYSGKPLANYWMHNGFLNVDGEKMSKSLKNFFTAR
                     DILAEYDAESIRFFFLSKHYRSPIDFSRELMDESQRAVANLYSSLAEINYPENAENRR
                     PTACGTAQELQNLEQEFILAMDDDFNTAKAISLLFELNRKVKNTSATMADRISAAIKM
                     VELGSVLGFFQNLEAKLKQKMPDLSKDLICLILSYRKEARDKKDWALADKIRNDLAQL
                     GIEIKDTPEGVKWTLKEKK"
     gene            complement(3269..7105)
                     /gene="porU"
                     /locus_tag="PLG69_03890"
     CDS             complement(3269..7105)
                     /gene="porU"
                     /locus_tag="PLG69_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPI42684.1"
                     /translation="MKRFFLAVILVCSLAFLNAKITLIRCSETELLVEYTIEPFKITA
                     EGEFSRIIAENMGHNSIPGTPSIPYTEFKVGIPPNGSINCSVLNSNTTTIILESKLIP
                     VPEIKSGEEVDYYLYNISPEAYQPQRGEFIIQLPETNFRGYHFVPVTLHPFVYDGNKN
                     LTVTTKATIKINISGQTNFRSLEESDKGADLLLAQLINSQQAKNWRSNPRPVINYAPF
                     GLSNWWMRIETNRPGIYRINYSDLISLPLNDIDPTTIRIFSTSGKVLGNNINNNGAEF
                     KEIPIFISGEADHSFNEQDYILFYGSFRDGYEQNAEVQSDPLYYNPYSQNQVFWLTFG
                     GSFSGNPLRIQTVSSPSTYDFSLTSTPVIKQIETETHRREKYGFTWFSETFFGNNTTD
                     YILTTELSDVSGSDDQKIQLRLCQESVGEGTVHKIQISVNGMPVYNNQDLHTLDFTWT
                     GTNYFYIDRPISNLVNGINTLKIRVFRNSKDNLYFDYYRFSYQQNLNKGNAQKQFFHK
                     NSMLPTICKFNLSGDLSNTLVFRINNLYSVDLIPVADNCIIADGNSKTFYFMLKPGEA
                     YRPSVMQLVQPNDLTYESGQFDNIIVTPAEFLQQAQELANKYREIYQISSKVILVDDI
                     YNQFNGGHIDPAAIRLALKYYYFNLPAPRITSLTLLGIGTMDWRNFSGFSAPKNKMIV
                     WQGSYIVSDDYFGMFNTSYYPELAIGRYPVKNTNELNIMLQNFTNYTLNPTPGWWRNS
                     MVFIADDLTNGDTNYEYVLTQYAESNSNDVHPSNLVDKIFAMEYEYDEFQNKPKARDD
                     LFKAINEGRLLMHYTGHGSYDKLGAEDYMNGATDMGRFANEGKLTFFIAAACEVSQFD
                     YWGFDCLAQKVVLLNNLGAIASLSATRKSFADNSSILLKYVLESLAYNRNPLGYCIMD
                     GKFRYTGGATNNEMYILFGDPVIRVVPPERDSTMLVVTSTDEDILHSRETAVINGSFA
                     NSNLSGITEIKTFDSKRAYSLGPATNVTKMGNQFYRGSSEVTSSHYNSSFIVPDDIVS
                     GSTGLIVAYLWDETGKKDYSNYYYPLQLSDQAVDIANPDVPQINIYLGSKDFRPGDIV
                     GTNTTLYADISDSNGINITGSSGHNILLVIDNSLQPTAITEYFNYDKNSCTTGTLIYP
                     LKDLSEGMHTIQLIAFDNFNMPAVATTTFVAKKSSELAIENLLIYPNPISKDGYITFM
                     LSMDAEVNIGIYTIRGKRIANIKIYGKQGFNKVYFNGRDDKGDTLANNTYFVKVQARS
                     ANGKKVEKTEKMVIYK"
     gene            complement(7102..7254)
                     /locus_tag="PLG69_03895"
     CDS             complement(7102..7254)
                     /locus_tag="PLG69_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42685.1"
                     /translation="MLLLLVSTIENITLALGIMVQGMLGIFIFMAVFYLLIYALEHLF
                     RPKEKP"
     gene            complement(7267..7731)
                     /locus_tag="PLG69_03900"
     CDS             complement(7267..7731)
                     /locus_tag="PLG69_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPI42686.1"
                     /translation="MKLTLLQIGKTKDKWLLEGISEYRKRISAFAKFEIIELPDESIK
                     HSGNAESVKTKEASTILKYIKPEDYVILLDEQGEQKNSLEFADFLISLSERKNVVFIT
                     GGVFGVDKSIWQRANNVLALSRLTFTHRLVRLILCEQIYRALTIINNRNYHK"
     gene            complement(7740..8639)
                     /locus_tag="PLG69_03905"
     CDS             complement(7740..8639)
                     /locus_tag="PLG69_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPI42687.1"
                     /translation="MFKNFAELLSYVKQNRKGTAIIAGAQIESAIEAGVMAKKEGLCE
                     SILVGDKPEIIRLIQQKAPDLVNAFEIIDTGSDLRKAAETAVALAKEEKGDLILKGKM
                     ESSVILKAALDKEKGLKVSEVISDVFVYEHPEGLKIQTDGGINILPELNEKIAIVKNA
                     VQVAHALGNPKPKVAMVCAIETVNPKMPATIDAALIAKMNERGQIPGCIIEGPLAFDN
                     AVDKEAARIKGLTSEVAGAADILVFPNIETGNVHGKMLTYYCHYKIAHVTIGTKVPIL
                     IPSRADSGETKMLCMALALATMS"
     gene            complement(8714..9592)
                     /locus_tag="PLG69_03910"
     CDS             complement(8714..9592)
                     /locus_tag="PLG69_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPI42688.1"
                     /translation="MSNKESVFADKALQNYLNEISKFKTLSREEEHDLAIKAKNGDMD
                     AMNKLIQANLKFVVKIASRYQNRGLSLSELISEGNIGLIKAIEKFDPDKDIKLISYAI
                     WWIKQRIMLAVSEKSSLIRVPMGKSSAAHRVKLTQDRYFTETGKIATPDELSEKMNIA
                     EKTIEQLQNKLPETSSFDDIITTSDSQDFTAKDLLEDKDTLDPQSLYYQERMQEKIQN
                     AISKLDAREADIIRNYFGLNESHESRNFAQIAEKLGLSRERVRQIQKEALKKIMREIK
                     PEEDEFVDRFLEKYHY"
     gene            complement(9603..10772)
                     /locus_tag="PLG69_03915"
     CDS             complement(9603..10772)
                     /locus_tag="PLG69_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPI42689.1"
                     /translation="MQIMLILPLGLSAEDQIHIIKKGDTLYSLSKRYGTTVDELKRLN
                     NLSDNNLSIGQKLIVKKDTEPIKPVAPVQVTKPVTPEISGGTSSPQISSLPGAETNPS
                     LSPAKKIPADYYYTVKAGDNLYRIAVNHNITLGELLAWNNLANSSIPIHPGDKLVIKN
                     PAELPETKIVPEEIITESNPVISTVPAKNDTVLIERVYVVQKKDTLYRIATNNGMTVE
                     ELMQLNNLTSPDIKVGQKLYLSGKPRPEITTPPEVLTEEEIMKREKIRTDLIMPVEGK
                     VISEYGLRNGRPHKGIDLGAKAGTPIYAVLDGTVVYSGVQGSYGNVIVIEHPDFVMTV
                     YAHNEKNLVNVNDVVKQGQQIGTVGSTGNAQGTHLHFEYRLKGKAINPRKVLPLP"
     gene            complement(10792..11061)
                     /locus_tag="PLG69_03920"
     CDS             complement(10792..11061)
                     /locus_tag="PLG69_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPI42690.1"
                     /translation="MPTWELYANGRVQGVGFRYYVWKIASELGIKGYVQNQWDGTVKI
                     VAQAEEHTLQTFQQIVSQGTKYAKVKELQIYKLDSVEKYNDFEIR"
     gene            11242..12960
                     /locus_tag="PLG69_03925"
     CDS             11242..12960
                     /locus_tag="PLG69_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPI42691.1"
                     /translation="MNLLHIINLVGGLALFLFGINKMSDSLQAVAGSEMRRILKVLIN
                     TPLKGVLVGLGVTVLIQSSSATTVMMIGLVNTGIMTLNQAVGVVMGANIGTTITAQLI
                     AFNIGQYAFLFVILGVTLLFIRKSKTMEHWSQILIGFGLLFVGLNVMGNSVSPLQNSI
                     TARNLMIRLASNPILSVLVGTCFTMLIQSSSASIGIVMVLASTGLIPFEGALYLVFGD
                     NIGTTITAWLAALSTGNTAKRVAMVHTLFNVFGTIIFGILTYLGIYTLLINWITPGNV
                     YAGQNIARHIANGHTMFNVINMILFLPFANQLAKLATKIIPPDKVETISLGEPKHLNY
                     TIISNSDLAINQSIKEMREMLRLVRMELMLAYQAFKEKDYKKQMRVSRIEKAIDNLQR
                     EITLYLVAVNEKTNAEDIIHKIPALLHTVNDIEKIGDFTEEINKILNEQIIAKKHHLS
                     PKFISIIDELHSKLLYMLDLSIDYLVELKEDYIYKIIEMEGRINETHHHLREEILSQV
                     QNGKCNAAEGLNTIDYIDEVEEIADKLKNLVKAGTHNFVYNRNFVKETEEEEKTENDK
                     ELTDVF"
     gene            complement(12957..13925)
                     /locus_tag="PLG69_03930"
     CDS             complement(12957..13925)
                     /locus_tag="PLG69_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPI42692.1"
                     /translation="MNIYETVQEELAKEATAIQKVAKQLSKESLEKAFDLLCKCKGKV
                     VLTGMGKTGIIARKISATLASTGTTSIFLHAAEGIHGDLGMIESGDVVIAVSNSGNTQ
                     ELINLIPFLKFKYVPIIAITGEPNSQLAKNSDVVLNCHIPKELEPLGLVPTASTTVAL
                     AVGDALAIALLKHKNFQLKDLAKFHPGGTIGKKLLLRVSDLMHSGKELPVIEEKAKMS
                     EAIMEMTSKKLGCTAVTNKDGKLTGMITDGDLRRQLHNKGNSLLSYTAKDCMTANPKT
                     LKPEVLAIEALNLMETYKITMIPVVDENNVPVGMLHMHDLITAGVI"
     gene            complement(13922..15823)
                     /gene="thrS"
                     /locus_tag="PLG69_03935"
     CDS             complement(13922..15823)
                     /gene="thrS"
                     /locus_tag="PLG69_03935"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPI42693.1"
                     /translation="MIKITLSDGSIREYSAPVTVLKIAEDISPKLAEEAICAEVNNKM
                     VDINYPIDTDATVVIHTFHTEKGKEVYWHSTAHLMAQAVKQLFPEVKIAIGPAIEQGF
                     YYDFDRDESFTEEDLQNIEAKMQELSTQALPYYRTELSKEEAIKLFSEMNETYKLELL
                     EDIPEGEIISSYRQGDFIDLCRGPHLPDTGKIKAIKLLRTSGAYWRGDEKNKMLKRIY
                     GISFPTEKELTNYLHFLEEASKRDHRKLGKDLDLFSISDEVGPGLVLWHPKGAMVRHL
                     IETYWKEQHLKKGYQLVYTPHLGRANLWETSGHLDFYKENMYSKMDIEGQDYYIKPMN
                     CPFHISIYNSSLHSYRELPIRLAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICTPE
                     QMDMEVDKLIVFSLDMLKAFGFKEFLIYLATKPDDCVGEKEDWDKATASLANSLQKLG
                     LEYKVDEGGGAFYGPKIDIKIKDALGRAWQCTTIQFDFNEPERFDMTYIGADNSAHRP
                     YMIHRAVLGSVERFFATLLEYHSGNLPLWLAPVQIMLIPITDAQLNYARKIEEQLLEQ
                     GFRCELDSRSEKMGYKIRSAEKNKIPFMCILGKNEENNNQVTLRQHTVGDLGAMNLEA
                     AITILRKEL"
BASE COUNT         4847 a         3038 c         2857 g         5191 t
ORIGIN      
        1 ttattccgaa actcataact atccacaaat tagtgctttc ctaatagaca ccgtaacgac
       61 tccctaacga ctccgtaaca cttccctaac acttccttaa ttctcgttac aggattgcta
      121 caaaattgtt aaagggctat tgatttgtcc agaaaggata aatccctctg gattcatcta
      181 acaaaagaca gcagaattca atctgggata atgaataagg aggagaatgt tttggcaact
      241 tccaatctgc taaagagttc gcaaaacttt gaaaaaaagc gttctttttg tctacaccaa
      301 ataatacatt cggtttttac aaaattgaaa ttaggcaaat acagaataca taattatctt
      361 tagaaacctt cagtttctat gataaagagg aaattgctat cccattttat gttaggaatt
      421 gatatgcact tcaaattctg ccagccattt acggttagct gattgaattg ctattggctg
      481 tttgccggaa tgtttgccga aatttttcct gcggttatca aataggcaaa tattttcctg
      541 taaagaggtc atccagcgtt cgctatttaa ctgcagaata gaaagatagg tattaagaaa
      601 gagggtaact gaactccgga aaaattcacc gggaaaatcg gcagcccaaa acaataaaag
      661 atgggtaata tccttttccg gataggcaaa ttcactttcg gtaaagtcaa taaaataata
      721 ctttccctgg cagaaaagaa tgtttttagg cgcattatct ttatggcata agcacttatc
      781 gttttggaga gtagcccaat gaaagttggc aatagtttca gcaagaagag caggttggaa
      841 attatttaat tcatcaagat aggggatacc cgttattttt tccattttta tccagtaggg
      901 ttctgcatag tccagcagtt ttggcaacat cggtaaattt aattgatagg caaaaagttc
      961 tgcctgaaag tgtttatagc tactgtagta tttaataaca caatcaggca gaacaataat
     1021 agagctgaat ttattcttat tcaagctgaa gacggataat ctgattatcg ctgaaaagag
     1081 ggacaatcaa ctgatttgct ggaagatagt aaaaaatatc cgctgcatct ttaatgcctt
     1141 tgaaagtggg cagatagcga ttttgttcct gcggaatttc aaaaatcata tcctgacccc
     1201 aactggtgat ataaattctg cccaaatcat caatagcaat gccgtccagg taatcataaa
     1261 tagtatcctt aaaaatgctg atgcggttat ctaaagtgct gatagaaaga atgggacttt
     1321 tttccttaaa gcagaccaca aacatctgat ctctgggcat atcataaact atgccgttgg
     1381 gttttttcag cagaggcgaa gtcaactttt gcattttatt agctataggg tctatcacat
     1441 atacacagtc cccgccggta tccgtagtgt aaataattcc tgttttggta aaagcgaggt
     1501 cgtttagcat aacagctccg ggaattttga gggtatctat gataaccgct ttttccggat
     1561 caacaatttt gatagcggtt ttatccgcca catacagttt tccggaatga atgataatgc
     1621 ctcgtggttc ttccagtcct ttatctaaaa agggagaata ttgttcatcc aaacttaagg
     1681 caacgatact tttacctaaa gtattggaga tgagaaagcg gtttccgaca gcatcaaaag
     1741 caatactttc gggtccgttt aacgggtctt tcctgctcag taaaattaaa gtgataattg
     1801 cagctactaa aaccagcagg ataagaataa gatataaggt tttagctttc attttttctc
     1861 ctttaaagtc catttaactc cttcgggagt atccttgatt tcaattccca actgagcaag
     1921 gtcattcctg attttatcgg caagagccca atcttttttg tctcttgctt ccttacgata
     1981 agataatata agacaaataa ggtctttaga caaatccggc attttctgtt tcaattttgc
     2041 ttccagattt tggaaaaacc ccagaacaga acccaattcc accattttta tggcagcgga
     2101 aattcggtct gccatagttg cagaggtatt ttttaccttc cgattcagtt caaataaaag
     2161 agaaatggct ttagcagtat taaaatcatc atccatagcc aaaataaatt cctgttccag
     2221 attttgcagt tcttgcgctg ttccgcaagc agttggacgc ctgttttccg cattttccgg
     2281 ataattgatt tcagcaagag aactatacaa attggcaaca gcacgctgtg attcatccat
     2341 caactcacgg ctaaaatcaa tgggactgcg ataatgcttg gaaagaaaga aaaatctgat
     2401 tgattcggca tcatattcag ccaaaatatc acgggcagta aagaaatttt tcaagctctt
     2461 gctcattttt tccccgtcca catttaagaa accattatgc atccagtaat tggctaaggg
     2521 ctttccacta taagccattg cctgggcaag ttcgttttcg tggtgcggga aaatgaggtc
     2581 tattcctcca caatgaatat caaatgtctc accaagatat ttttgactca taactacaca
     2641 ttccgtatgc caaccaggac gtccctttcc ccagggactt tcccaaaccg gttctccagg
     2701 tttgcctttt ttccataaag taaaatctgc aggatgccgt ttgttataat tttccataac
     2761 tcttgcacct gccagctgtt cttccgtttt tttacccgaa agacagccgt aggaaggcaa
     2821 attttccgta gcgaaataga catccccttc cacttcataa gcataacctt tcttttccag
     2881 ttccgcaata aaggcaatta tatcatccat tacttccgtt gctttaggtt gataagttgg
     2941 cggtttaatt cccaaagcgg aagtatcttc cagaaaagca cgggtatatt tttccgcaac
     3001 caaagtgaaa ctgatatttt cttcatttgc ctgagcaatg attttatcgt ctatatcggt
     3061 aatattttga acataagtaa cttcgtaacc gcgataagca aaatagcgac gaactacatc
     3121 aaaaaagata aaagcgcggg cattaccaat atgaaagtaa ttataaacag taggaccaca
     3181 ggcataaatg cctacttttc caggagttat gggctggaat tcttcttttt tacgggtttg
     3241 agtgttgtaa agtaacattc tattatacct atttatagat taccattttt tcggtttttt
     3301 ccaccttttt gccattggct gaacgggctt gaacttttac aaaataagtg ttattagcta
     3361 aagtatcgcc tttatcatca cgaccgttaa aatagacctt attaaatccc tgtttgccat
     3421 aaatcttaat attggcaatg cgtttgccgc gaatagtgta aattcctata tttacttcgg
     3481 catccataga aagcataaag gttatataac catctttgga aataggatta ggatagatca
     3541 gtaaattttc aatagccagt tcggaactct ttttagctac aaaggtggtg gttgccacgg
     3601 caggcatatt aaaattatca aaagcaataa gttgaattgt atgcattcct tcacttaggt
     3661 ctttcagcgg ataaatcaaa gttcccgttg tgcaggaatt tttatcgtaa ttaaaatatt
     3721 ccgtaattgc tgtgggctgt aaagaattat caattaccag aagaatatta tgccccgaag
     3781 aaccggtaat attaatgccg ttggaatcgg aaatatctgc atataaggta gtatttgtgc
     3841 caactatatc cccgggacgg aaatctttgg aaccgagata aatgttaatt tgcggaacat
     3901 caggattggc aatatccact gcttgatcgc ttaactgcaa aggataatag taattagaat
     3961 agtccttttt acctgtttca tcccataaat aagccacaat aagtcctgtg gaaccgctga
     4021 caatatcatc cggcacaatg aaagaagaat tataatgaga agaagtaact tcactgcttc
     4081 cccgatagaa ttgatttccc attttagtaa cattagtagc cggaccaaga gaatatgctc
     4141 ttttagaatc aaatgttttt atttctgtta tgccggaaag atttgagtta gcgaacgaac
     4201 cgttaataac agccgtttca cgcgaatgca aaatatcttc atcagtggaa gttacaacca
     4261 gcatagtgct gtctcgttcc ggtggaacaa ctctaatcac aggatcccca aaaagaatat
     4321 acatttcatt attagtcgca ccaccagtat aacgaaattt accatccata atacaataac
     4381 ccaaaggatt acgattataa gctaagcttt ccaatacata tttaagcaat attgagctgt
     4441 tgtcagcaaa acttttgcgg gtagccgaca aagaagctat ggctcctaaa ttattcagta
     4501 aaactacttt ttgagctaaa cagtcaaaac cccaataatc aaattgagaa acctcgcagg
     4561 cagcggcaat aaagaaagtt agtttacctt cattggcaaa tcttcccata tcggtagctc
     4621 cgttcatata gtcctcggct ccgagtttat cataggaacc atgccctgta taatgcataa
     4681 gcaatctacc ttcattaatg gctttgaata aatcatccct ggctttgggc ttattttgaa
     4741 attcatcata ttcatattcc atagcaaata tcttgtctac caggttactg ggatgcacat
     4801 cattgctgtt gctttcagca tattgagtga gaacatactc gtaattcgta tccccgtttg
     4861 tcaggtcatc tgctataaat accatagagt tacgccacca acctggagtt ggattaagag
     4921 tataattggt aaaattctgc aacataatgt ttagctcatt agtgttttta acaggatacc
     4981 ttcctatagc tagttccgga taataactgg tgttaaacat tccgaaataa tcatcagaaa
     5041 caatataact tccctgccaa acaatcattt tatttttagg agcggaaaag ccggaaaaat
     5101 tccgccagtc catagtgcca atacctaaca aagttaaaga ggtaatgcgg ggtgcaggta
     5161 aattaaaata ataatacttt aatgccaaac ggatagctgc agggtctata tgtccaccat
     5221 taaattgatt ataaatatca tctaccaaaa tgaccttact gctaatttga tagatttccc
     5281 tatatttatt ggcaagttcc tgcgcctgtt gtaaaaattc tgcaggtgtc actattatat
     5341 tatcaaactg accgctctca taggttaaat cattgggttg aactaattgc attacagaag
     5401 gtctatatgc ttctcctggt ttcagcataa agtaaaatgt cttgctgtta ccatcggcaa
     5461 taatacaatt atcggcaacg gggattaaat ctacactgta aagattattg atccgaaaaa
     5521 caagggtatt gctgaggtct ccactcaaat tgaacttaca aatagtggga agcatagagt
     5581 ttttatggaa aaattgcttt tgagcattgc ctttatttaa attttgttga tagctaaagc
     5641 gataataatc aaaataaagg ttatctttac tattgcggaa aactcttatt ttaagagtat
     5701 ttatgccatt aaccagatta cttataggac gatcaatata aaaataattc gttcctgtcc
     5761 aggtaaaatc caaagtatgt aaatcctgat tattgtaaac aggcattcca tttaccgata
     5821 tttgaatttt atgaactgtt ccttctccaa ctgattcctg gcataaacga agctgaattt
     5881 tttgatcatc gctaccagag acatcagaga gttctgtagt aagtatataa tccgttgtgt
     5941 tattaccgaa aaaggtttca ctaaaccagg tgaaaccata tttttctcgg cgatgggttt
     6001 ctgtttctat ttgtttgata acaggagtag aagttaaaga aaaatcataa gtgcttggag
     6061 aagaaacagt ttgaattctt aaaggatttc cactgaagct acctccgaaa gttagccaga
     6121 aaacctgatt ttgtgaatag ggattgtaat ataggggatc ggattgaact tccgcattct
     6181 gctcatatcc atccctgaaa ctgccataaa acaaaatgta atcctgttcg ttaaaactgt
     6241 ggtctgcttc tcctgaaata aaaataggaa tttccttgaa ctcggctccg ttattattta
     6301 tattgttacc taagaccttg ccggaagtgc tgaaaatacg aatagtagta ggatcaatat
     6361 catttaaagg caaactgata aggtcggaat aattaattct ataaatacca ggacgattag
     6421 tttctattct catccaccag ttacttaaac caaagggagc ataatttatt acgggacgag
     6481 gattactacg ccagtttttt gcctgctggg aattgattag ttgcgccaaa agaaggtctg
     6541 ctcccttgtc gctttcttcc aaactgcgaa aattggtctg acctgaaata ttaattttga
     6601 tagtagcttt ggtagttacg gtaagatttt tgttgccatc gtaaacaaaa ggatgtagtg
     6661 ttaccggcac aaaatgataa ccccggaagt ttgtttccgg aagctgaata ataaattctc
     6721 ctctttgggg ttgatatgct tcgggagaga tattgtagag gtaataatct acttcttcac
     6781 cactttttat ttccggaaca ggtataagct tggattccaa aataatggta gttgtattac
     6841 tattcagaac tgaacaattg atagagccat tgggaggaat acctacttta aattcagtgt
     6901 atggaatgga aggtgttccg ggaatactgt tgtgtcccat attttcagct atgatgcgac
     6961 taaattcacc ttccgcagta attttgaagg gttctatagt atattccacc aataattcgg
     7021 tttctgaaca gcggataagg gttattttgg cattcagaaa agcaagagag caaacaagaa
     7081 taaccgccag aaaaaagcgt ttcaaggttt ctccttaggt ctgaataaat gctccaaagc
     7141 atatatcagc agataaaaca ctgccataaa tataaatatc cccagcatac cctgaaccat
     7201 tattcccagg gcaagcgtta tattttctat agtagagaca agtaacagta acattatttc
     7261 ctctttttat ttatgatagt tgcggttatt aattatagtt aaagcacggt aaatctgttc
     7321 acatagaata agacgaacca acctatgggt aaaagttaat cttgataaag ccaaaacatt
     7381 gtttgctctt tgccaaatag atttatctac accaaaaaca ccacctgtta taaagaccac
     7441 attctttctc tcagataagc taatcaaaaa atcggcaaat tcaagggaat ttttttgttc
     7501 gccttgttca tccaacagaa taacataatc ctcaggtttg atatatttca ggatagtaga
     7561 tgcttctttg gttttcacgc tttctgcatt acctgaatgt ttgatgcttt catcgggcaa
     7621 ttcaataatt tcaaatttgg caaaggcaga aatccttttc cggtattccg agataccttc
     7681 cagtaaccat ttatcctttg ttttaccaat ttgcagaagg gttaatttca ctgttttttt
     7741 caggacattg tagctaaggc aagtgccata cacagcattt tggtttcgcc tgaatctgcc
     7801 cgtgaaggta tcaaaatagg cactttagtt ccaatagtta catgggcaat tttatagtga
     7861 caatagtagg taagcatttt gccatgcaca tttcccgttt caatattagg gaagaccaaa
     7921 atatctgctg ctcctgcaac ttcggaagtg agacctttta tgcgtgcagc ttctttatcc
     7981 actgcattat caaaagctaa gggaccttct atgatgcaac cgggaatttg acccctttca
     8041 ttcattttag caatcaaagc cgcatcaatg gtagcgggca ttttgggatt gactgtttct
     8101 atagcgcaaa ccatagctac tttgggtttt ggatttccta aagcatgagc aacctgcact
     8161 gcatttttaa cgatagctat tttctcattc agttcgggca ggatattaat tccaccgtcg
     8221 gtttgaattt ttagtccttc cggatgttca taaacaaaaa catcggaaat tacctcgctt
     8281 actttcaagc ccttttcctt atccaatgca gcttttagga taacggagga ttccattttc
     8341 ccttttaata tgaggtcacc tttttcttct ttggctaaag caacggcagt ttcagctgct
     8401 tttctcaaat cactgccggt atcaataatt tcaaaggcgt ttaccaggtc gggagctttc
     8461 tgttgtatca gacggataat ttctggttta tcgccaacca ggatactttc acataagccc
     8521 tctttttttg ccataacacc tgcttctatg gcagattcaa tttgggctcc agcaataata
     8581 gcagttcctt tacggttttg ttttacatag cttagcaatt cggcaaaatt cttaaacata
     8641 ctgatatatc tcctcttttt tagttaaaat gaaaaaatta tctgccctaa ctgggtagaa
     8701 agaaaacagg tgcttaataa tgatactttt ccagaaacct atctacaaat tcatcttctt
     8761 cgggttttat ttcccgcata attttcttta aagcttcctt ttgaatttgg cgaactcttt
     8821 ctctggataa ccctaatttt tcagctattt gggcaaaatt tcgggattcg tgagattcat
     8881 ttaagccaaa ataattccga ataatatctg cttcacgagc atctaattta ctgatcgcat
     8941 tctgaatttt ttcttgcatt ctttcctgat agtataaact ttgggggtct aaggtatctt
     9001 tgtcttccag caggtcttta gccgtaaaat cctgactatc tgaagtagta ataatatcat
     9061 caaaagagct tgtttccggt aacttgtttt gcagttgttc tatagttttt tcggcaatat
     9121 tcattttttc gcttagttca tcgggggtag ctatttttcc tgtttccgta aaatatctgt
     9181 cttgcgtaag tttaactctg tgagctgcgc tggattttcc cattggaacg cgaataagag
     9241 aacttttttc gctaacggca agcattattc tctgttttat ccaccagata gcataggaaa
     9301 taagcttaat atccttatcg ggatcaaatt tttcaatagc tttaatcagc cctatattgc
     9361 cttcgctgat tagttctgaa agggataatc cccgattttg atagcgtgag gcaattttaa
     9421 ctacaaattt cagatttgcc tggattaact tgttcattgc atccatatcc ccgtttttag
     9481 ctttaatagc aaggtcgtgt tcttcctcac gggaaagggt tttgaacttg gaaatctcgt
     9541 tcagataatt ttgcaatgct ttatccgcaa aaacactttc tttattgctc attttttatt
     9601 ccttagggta aaggaagaac tttacggggg ttaatggctt tacctttcaa acggtattca
     9661 aaatgtaaat gagtgccttg agcattgcca gttgaaccaa ctgtaccaat ttgctgacct
     9721 tgctttacta catcattcac atttaccaga tttttctcgt tatgagcata aacagtcatc
     9781 acaaaatcgg gatgttcaat aacaattaca ttaccataag aaccttgaac tcccgaataa
     9841 acaactgtgc catccaaaac agcataaatt ggcgtgccgg ctttagcacc taaatcaatt
     9901 cctttatgag gacgaccatt acgcaatcca tattcggaaa taacttttcc ttccacgggc
     9961 ataattaaat cggtgcggat tttttccctt ttcataatct cttcttcagt tagaacttcc
    10021 ggaggagtag ttatttccgg acgcggttta ccagaaagat ataatttctg tcctacttta
    10081 atatctggag aagttaaatt attcaattgc atcagttctt caacagtcat tccattattg
    10141 gtggcaattc tgtaaagtgt atcttttttt tggactacat agaccctttc aatcagcact
    10201 gtatcatttt tggcaggaac cgtagagata acaggattgg attctgtgat tatttcttca
    10261 ggaacaattt tggtttcggg taattcggca ggatttttaa tcaccagttt atcacctggg
    10321 tgaataggaa ttgagctatt ggcaaggtta ttccaagcaa gaagctctcc taatgttata
    10381 ttatggttta cggctatacg atagaggtta tcaccggctt taacagtgta ataataatcc
    10441 gcgggaattt ttttggcagg ggagagggaa ggattagttt ctgcacctgg caaagaggaa
    10501 atctgtgggg aagaagtacc tcctgaaatt tccggagtaa caggtttggt aacttgaacg
    10561 ggggcaaccg gtttaattgg ttccgtatct ttcttcacga ttaatttttg accgatggaa
    10621 agattgttat cggataagtt attaagccgt ttcaattcat ccacagttgt gccataccgc
    10681 ttacttagag aatacagggt atctcctttt tttattatat gtatctggtc ctcagcagat
    10741 aaacccaaag gtaaaattaa cattatctgc attataacca accacagtaa tctatctaat
    10801 ttcaaagtca ttatattttt caacgctatc caatttataa atttgcagtt ctttcacttt
    10861 agcatatttg gttccttgcg aaacgatctg ttgaaaggtc tgcaaagtgt gttcttctgc
    10921 ctgggcaacg attttaaccg tgccgtccca ctggttttga acataacctt taattccaag
    10981 ttcggaagca attttccata cataataacg gaatcctact ccctgaactc tgccgtttgc
    11041 ataaagttcc caagtaggca tatatcacac ctttagatta tttgttctgc ataaatttta
    11101 tgcccgaatt tgtcaagtat tcgttttcta ccatttttga aaaggtgata tttttcctta
    11161 tatcaagcaa aaatatactt gaccataaca gcgaggttaa aaatatatag acattatggt
    11221 taaaaggatt gtaataattg gatgaactta ttgcatatca ttaacttagt agggggcttg
    11281 gcactttttc ttttcggcat caataaaatg agcgatagtt tacaagcagt tgccggttcg
    11341 gaaatgcgtc ggattttgaa agtgctgatt aatacacctt taaaaggggt cttagtgggt
    11401 ttgggtgtaa ctgttttaat tcaaagtagt tctgctacca ccgttatgat gataggattg
    11461 gtcaataccg gaattatgac tttgaatcaa gcagttggtg tagttatggg agccaatatt
    11521 ggaaccacga ttacagctca gttaattgca ttcaatatag ggcagtatgc ttttttattt
    11581 gttattttag gggtaacctt acttttcatc cgcaaaagca aaacaatgga acattggagt
    11641 cagattctta ttggttttgg tttattgttt gtaggattaa atgtgatggg taattcggtt
    11701 tctcctttac agaattctat tactgctcgt aatttgatga tcagattagc ttccaatccc
    11761 atactaagtg ttctggtagg aacctgtttc acgatgttaa ttcagagttc ttctgcatct
    11821 ataggaattg ttatggtatt ggcatcaaca ggtctgatac catttgaagg ggctttatat
    11881 cttgtttttg gagataatat cggcacaacg ataactgcct ggctggcagc attaagtact
    11941 ggtaatacag caaaacgggt agctatggtg catactcttt tcaatgtatt tggcaccatt
    12001 atttttggca ttttaactta tctggggatt tatactcttt taatcaattg gattactccg
    12061 ggaaatgttt atgccggaca aaatatagcc cgtcacattg ctaatggaca tacgatgttc
    12121 aatgtgatta atatgatatt atttttgcct tttgccaatc agttggctaa gctggcaact
    12181 aaaattattc ctccggataa agtggaaacc atttctttag gcgaacctaa gcatttaaat
    12241 tacacaatta tcagcaattc cgatctagca ataaaccagt ccatcaaaga aatgcgcgaa
    12301 atgctgcgtt tggttagaat ggaacttatg cttgcctatc aggcatttaa ggaaaaggat
    12361 tataaaaaac agatgcgggt ttcccgaatt gaaaaagcaa ttgataatct acaaagagaa
    12421 ataactcttt atctggtagc agtgaatgaa aaaaccaatg cggaagatat tattcataaa
    12481 attcctgctc ttctgcacac tgtaaacgat attgaaaaaa tcggtgattt taccgaagaa
    12541 ataaacaaaa tcctgaacga acagataata gctaaaaaac atcatctttc tcctaagttt
    12601 atttccataa ttgatgaatt acattccaag ctgctgtata tgttggattt atctattgac
    12661 tacctggtag agttgaaaga ggattatatt tataagatca ttgaaatgga aggacgcatc
    12721 aatgagacac atcatcacct gcgtgaggaa attctttctc aggttcaaaa tggaaaatgc
    12781 aatgcagcag agggcttaaa tactattgat tatattgatg aagtggaaga aattgccgat
    12841 aaactgaaaa atttggtaaa agcaggaact cataattttg tttataatcg taattttgtg
    12901 aaagaaactg aggaagaaga aaaaacggaa aacgataaag aattaaccga tgttttttaa
    12961 ataacgccag ctgttatcaa atcgtgcata tgtaacattc ctacagggac attattttca
    13021 tccacaacag gaatcatcgt tattttatag gtctccatca aatttaatgc ttcaatagct
    13081 aaaacctcag gtttcaatgt tttaggattg gctgtcatac agtcctttgc cgtatagctt
    13141 aaaagagaat tacctttatt gtgcagttgt cttcttaaat caccatcggt tatcattccg
    13201 gtcaattttc catccttatt agtaactgca gtacagccaa gttttttgga agtcatttcc
    13261 attatggctt cggacatttt tgccttttct tcaataaccg gaagttcctt tccactatgc
    13321 attaaatcgg aaactcttaa aagcagtttt ttaccgattg taccgccagg atggaacttt
    13381 gccaaatcct tcagctggaa atttttatgt ttcagtaagg caatagctaa ggcatctcct
    13441 acagcaagag ctacagttgt ggaagcagta gggactaatc ctaagggttc caattcttta
    13501 ggaatgtgac aattgagcac tacatcggaa ttttttgcca gttgtgaatt tggttctccg
    13561 gttatagcta ttataggaac atatttgaat tttaaaaaag gaattaaatt tatcagttcc
    13621 tgtgtatttc cgctattgga aacagcaatt actacatcac cagattcaat catacctaaa
    13681 tcgccatgaa taccttctgc ggcgtgtaaa aaaatggaag ttgttcctgt agaagctaaa
    13741 gtagcgctaa ttttacgggc aataataccg gtttttccca ttcctgttaa gaccactttt
    13801 cctttacact tgcaaaggag gtcaaaagct ttttctaagg attctttact aagttgtttt
    13861 gctacttttt gaatagcggt tgcttcttta gcaagttctt cctgaacagt ttcgtaaata
    13921 ttcataattc ctttctcaag atagtgattg ctgcttccaa attcatagcg ccaagatcgc
    13981 caacagtatg ttgacgtaaa gtgacctgat tattattttc ttcatttttt ccgagaatac
    14041 acataaaggg aattttattc ttttccgcag aacgaatttt gtaacccatt ttttcactgc
    14101 gggaatccaa ttcacaacga aaaccttgtt ccagtaattg ttcttcaatt ttgcgggcat
    14161 agttcaattg agcatcggtt ataggaatca gcattatttg gacaggagca agccaaagcg
    14221 gtaaattacc gctgtgatat tccagtaaag tagcaaaaaa tcgctctaca gaacccaaaa
    14281 cagctctatg aatcatataa ggtctatgcg cagaattatc cgcaccaata taagtcatat
    14341 caaatctttc gggttcatta aaatcaaatt gaatggtagt acattgccaa gctctgccca
    14401 aagcatcttt aattttaata tctattttag gtccgtaaaa agcaccgccg ccttcatcaa
    14461 ctttatattc caaacccaat ttttgtaagg aattagccaa agaagcggtt gctttatccc
    14521 aatcctcttt ttctccaaca caatcatccg gtttagtggc aagataaatt aaaaattctt
    14581 taaaaccgaa tgccttcagc atatccagag agaaaacaat caatttatct acttccatat
    14641 ccatctgttc gggagtgcaa attatatgtg catcatcttg agtgaaacca cgaaccctca
    14701 ttagaccatg taaaacacca cttcgttcat atctataaac agttcccagt tctgccaggc
    14761 ggataggaag ttctctatag ctgtgtaagc tggaattata aatgctaata tgaaaaggac
    14821 aattcatcgg tttgatgtaa taatcctgac cttcaatatc cattttgcta tacatatttt
    14881 ctttatagaa atccaaatga ccgctggttt cccataaatt tgctcgtccc aaatgaggag
    14941 tataaaccaa ttgatagccc tttttcaggt gctgttcttt ccagtatgtt tcaattaaat
    15001 gacgcaccat agctcctttg ggatgccata aaaccaaacc tggtccaact tcatcgctta
    15061 tagagaaaag gtccaggtct ttgcccagtt ttctatgatc gcgtttactt gcctcttcca
    15121 ggaaatgaag atagttcgta agctcttttt ccgtaggaaa actgattcca tatatccgtt
    15181 tcagcatttt gtttttttca tcaccacgcc agtatgcacc tgaagttctc aataacttta
    15241 tggctttaat ttttccggta tcgggaagat gaggtccgcg acaaaggtca ataaaatcac
    15301 cttgacggta ggagctgatg atttctcctt ccggaatatc ttccagaagt tccagtttgt
    15361 atgtttcgtt catctcgcta aaaagcttta ttgcttcttc tttacttaat tctgttctgt
    15421 aatagggaag ggcttgtgtg cttaattctt gcatttttgc ttcaatattt tgcaagtcct
    15481 cttccgtaaa ggattcgtct ctatcaaaat cataataaaa accctgttca atagcaggac
    15541 cgatggcaat tttcacttca ggaaacaatt gtttcactgc ttgagccatt aaatgagccg
    15601 tactatgcca ataaacctct tttccttttt ctgtatggaa ggtatgaata actactgtgg
    15661 catccgtatc tataggatag tttatatcta ccattttgtt attaacttct gcacaaattg
    15721 cctcttccgc aagtttggga ctaatatctt cggcaatttt taaaactgta acaggagctg
    15781 aatattctcg tatactgcca tctgacaagg taatcttgat catttatatc tccatcatat
    15841 aattctttgc acaaaaataa ccttattttc cataaataat attctgtcaa gctgattaaa
    15901 gggattttac ttttaccaag agaaaaagag aaa
//
LOCUS       DAOPFL010000034        15879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_4199_length_15879_cov_94.945147, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000034 DAOPFL010000000
VERSION     DAOPFL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15879
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4199_length_15879_cov_94.945147"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="PLG69_03940"
     CDS             <1..1543
                     /locus_tag="PLG69_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPI42694.1"
                     /translation="DWSLQGNHHQDLPEKSSLNATINFVSNKRIWESSIDVDQSLAQR
                     LTSSVSYSKPIGSSYLNAGSYFTQDLINDTASLSLPSASFFLSSRPVSELFNVSSNSW
                     LSNFNYRYNVYLEHTGNLREKNYSLGDFFWDNTIDPADTIGIRMLNEHHLGIKQNAGI
                     SHNSNLFGWLTLGKNFDYTEAWMDRDRNDDKFARGYSWTASANARFNLYGLRNFKNFP
                     INSIRHIITPNIGYSYRPDTRKNSDLYSFGSIGVSNSQEASNLTFNLDNTWQMKYGKK
                     GNETKLNDLLFWRLGTNANLKKKDKPFSNIAHTLYFKPGSFNLGTLSFNEGKYKLSSL
                     KLSYSSQFSASQDPYKIQWDKMNLRNQYFSQGIVLSGSAPYNRYFSAPKNKSFEDFEN
                     SDSQKSVLDYMQETAGTNDWSFSISQNIYADKDIFHSATSSLRSSLTCKITQNWRLSY
                     TNYYNLKTNDMLSQTLSFSRDLHCWKMDVNITLRNDYWDYRISLFNTLLPDALRFQTH
                     DSKRY"
     gene            1524..2114
                     /locus_tag="PLG69_03945"
     CDS             1524..2114
                     /locus_tag="PLG69_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPI42695.1"
                     /translation="MTAKDIKRAIFLDRDGVISPDDFGYLSNPEEYHLYPYTIEALKI
                     FKKLNFLLFVVTNQSGIARGYFTIDDLEKVLAKMRSLLSAGGIELDGVYYSPYYKDGI
                     VPPYNIDHIDRKPGIGMFKKAYSEFHFQIEGSYMIGDRISDLGFGRNAHLKNILLLTG
                     NGEKDFIKILETDDLRPDFVCENLLTAAKLIRDYKL"
     gene            2111..2920
                     /locus_tag="PLG69_03950"
     CDS             2111..2920
                     /locus_tag="PLG69_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPI42696.1"
                     /translation="MKIFAHYSETKFKSFTFEEQIGALNKLILSLEKNLTDTEKRSQI
                     IEHILELYLLVEKPLPQKMQLFIKELNPHLSPHQLLNKLYFYCDGAIRKDSQMALQTE
                     KGKVILDPDIRQKAKQITVICDNLRSVFNVGSLFRTAECLGIGKIIVCGITPTPEHSN
                     MPKTAMGTEKLVAWQYFPSTNEAIAFCHQEGFFIYALETIDNAKSVFQTVYQFPLAIV
                     IGNEALGIESSILELCDEFITLPQLGWKNCLNVGVATGIVLYQILFGGFNG"
     gene            2913..3707
                     /locus_tag="PLG69_03955"
     CDS             2913..3707
                     /locus_tag="PLG69_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HPI42697.1"
                     /translation="MDNLAYFEKLLILLQEHSPVWQANIIESDGSTPAKTGMKLAIPL
                     KEEPFGNLGGGELEHTVIEKIRNDKPGKATIYIFNLSADGSKAGIATSMLCGGNVKVY
                     VEPLFSPDKLYIIGAGHCGKALGKLASLCGFYVVLIDNRKEIIDNLPEDCCHRAIHHD
                     YANLAEIIDFNPNTWIVIMTHGHLHDKEVLEQCLRKPFRYLGMIGSKTKAAATFTKLK
                     EKGFTPEELAKCHSPVGLSIGSNTPYEIAVSIMAELISLRNLQDKE"
     assembly_gap    4390..4489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4525..6309)
                     /locus_tag="PLG69_03960"
     CDS             complement(4525..6309)
                     /locus_tag="PLG69_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPI42698.1"
                     /translation="MAKTVNVFIDGHHLEVPETMTIIEAADKIGIYIPRLCYHPDLPP
                     TGNCGVCVVEIAGNPTPKRACCTPVGEGMKIVTNSKKLRSYRKTVVEMILSNHDVTCP
                     TCPANNKCELQSLANNLGVDSEALPNILVKTPADESSLSIIRDTNKCIGCGRCITVCN
                     EVQTVYALTFAERGIDTHIDTAFSQGMANSPCVNCGQCTVYCPTGALHERSELDAVWE
                     AILDPEKHVIVQEAPSIRVSLGEEFGMPLGSVTPKKMYAALRKIGFDSVMDTNFTADL
                     TIMEEGTECVTKLKAGEKRPLITSCSPGWIKFMETYYPDLADCVSTAKSPMSMFGVLS
                     KTYYAQEKGIDPAKIVSVAIMPCTAKKFEARRPELSDSGFQDTDYVLTTREFIRMIKE
                     AGIDFANIPDEEPDEGMSFYTGAGTIFGATGGVMEAAIRSAYYLVTGTDLQNVDIMAV
                     RGLEGVKEASVDVPGFGKVNVAVAHGLSNARKVFDRVREGLATKGESPWHFIEIMACP
                     GGCVGGGGQPYGNDIASRARRGLALYEEDRNLPVRMSHHNPEVLKVYEKFLGEPNSKL
                     ALKLLHTHYFKRSVFNGQVTAEITHKHG"
     gene            complement(6331..7893)
                     /gene="nuoF"
                     /locus_tag="PLG69_03965"
     CDS             complement(6331..7893)
                     /gene="nuoF"
                     /locus_tag="PLG69_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPI42699.1"
                     /translation="MKFYRSHILVSINETSLAAGVQEFITALRNELAKNDLAEEINIL
                     ETGPLGFFGRGICLTVYPENINYEGVKIEDIPELVQEHFLKGRPVKRLMVGVTEKFSP
                     KFNYENRIVLRNSGIIDPENIDDYIGAGGYVALEKALTNMQPNDIIAEVKKSGLKGRG
                     GAAFPAGLKWSFTAGLNVPQKYVVVNADEGEPGTFKDRLIMEGDPHQLLEGIILCARA
                     VGASKAYIYIRGEYKLCIARLEKAIKQAYDYGILGKNIFDSGFDLDIELKIGAGAYVC
                     GEETALIESLEGNRGNPRWKPPFPGVEGLWKAPTIVNNVETLANVPFIIAKGADEFLQ
                     YGTPDCPGTKVYTILGDVAYPGLCEVDMGTTLRTIINDYAGGMKKGFRFKAALVGGAA
                     GVILSDRLLDVKMDFTSLNQYSAVLGSGAILVLNEHQSIVDLLWSILRFFRHESCGKC
                     SPCKNGTQQLYQLISKIRKGNGTMEDVNLMLLIAETMQQTSFCALGQSPIMAVRSAIE
                     NFTDEFIEITKK"
     gene            complement(7893..8348)
                     /gene="nuoE"
                     /locus_tag="PLG69_03970"
     CDS             complement(7893..8348)
                     /gene="nuoE"
                     /locus_tag="PLG69_03970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPI42700.1"
                     /translation="MIKEICQKYAPRKDNLIQILHEIQDQDPQHYISPEAVDTVAEYL
                     DIPVNHIYGVLTFYTMYSTKPRGKNIIRLCESPPCYIKGSDNMLRKLKVLLGINIGET
                     TKDGLFTLEFTSCLGVCGNAPVMMINDDVYGDLTEEKVEEIIERIRGRR"
     gene            complement(8361..10220)
                     /locus_tag="PLG69_03975"
     CDS             complement(8361..10220)
                     /locus_tag="PLG69_03975"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPI42701.1"
                     /translation="MSLKRIDLLICCGSGCVSAGALKVKEQFHNVLKTKGLTNEINII
                     ETGCMGPCDYGPVIVIYPEGVFYKKVTPDDVEEIVNEHFLKGRPVKRLMLQDEEDKTF
                     LTQKEIPFYQKQVKVALANCGYIDPESIEEYIATGGYEALGTVLTTLTPQQTIDIIKK
                     SGLRGRGGGGFPTYLKWQMVHDNPSEEKYFICNGDEGDPGAFMDRSLLEGDPHRVLEG
                     MMIAAYATGAQNGYFYIRAEYPLAIKRIKMAIEKAKETGLLGKNIFGSEFSFEAEVRT
                     GAGAFVCGEETALIQSIEGQRGNPTPKPPYPAVQGLYKKPTVVNNVETLGNIATIILK
                     GADWFASMGTENSKGTKVFALTGDIRNTGLVEVPMGITLRELIFDVGGGMIGDHKFKS
                     VQLGGPSGGCLTTQHLDVPIDYESLKERGAMMGSGGVIVMNEEKCMVNVAKFFMDFCV
                     DESCGKCSPCKIGLKQMLEILKRIDSGYGKEGDIEELQQLGETISKISLCGLGQTAPN
                     PVLSTIRYFRDEYEAHIRDKSCANKVCPDLMHFEIDKDKCIGCSLCARKCPVSCISGS
                     REEKYTINQLPCIKCGTCMDVCPVKAISKIPGMHPEVKAMREAEELQKQNQEE"
     gene            complement(10236..10601)
                     /locus_tag="PLG69_03980"
     CDS             complement(10236..10601)
                     /locus_tag="PLG69_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPI42702.1"
                     /translation="MKTLADLKKIREKAQEEMKLRGGNTRIKIVVGMGTSGIAMGARE
                     VMKTFLEEIANRNLTDVLVTQTGEKGLSSLEPVVDIIEEGKPKVTYGNMTPEKVKKVV
                     VEHIVNGRIVSDYVVATGN"
     gene            complement(10601..11170)
                     /locus_tag="PLG69_03985"
     CDS             complement(10601..11170)
                     /locus_tag="PLG69_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42703.1"
                     /translation="MQDISLHLLDIIENSVRAKAKNIKIRIIRDVQDNILRLIVEDDG
                     VGMDSQTLAKAQSPFYTSKEEREKKVGLGIPLVKQNAEATNGSFKMTSEPGFGTVLTV
                     DFQLDHIDRMPLGNLKDTLLGAIIGHPEVDFTIKLISHERGVEKSFYFDTAAIKEELG
                     NIPLTYPDVIEYIDQSLLEGIQNTNMEDV"
     gene            complement(11184..11666)
                     /locus_tag="PLG69_03990"
     CDS             complement(11184..11666)
                     /locus_tag="PLG69_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPI42704.1"
                     /translation="MIPADQMQNDQYAHLKKVIEEKKKIRNPLIEILRSAQEIFGYLP
                     VEVQEFIAQELNIPVNQIYGVVTFYNFFTMTPRGKYNLNVCLGTACFVKGAPRLVQML
                     SEELGIQMGETTKDGIFTMSAVRCVGACSLAPVFVIGEETYGRIDSKDKIAEILKRYK
                     "
     gene            complement(11696..12433)
                     /locus_tag="PLG69_03995"
     CDS             complement(11696..12433)
                     /locus_tag="PLG69_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPI42705.1"
                     /translation="MQSLRADLHIHSVLSPCGGLEMSPNSIMQRLKQFGIQWLAITDH
                     NSMANCPAYQKVAEKEGLFFTWGVEIQSVEEIHLLAYFEDSSSAEKFNTELYESLFPL
                     DNDPDFFGDQVIIDENENILRVEPRALINSSQWNLNTVVEKVQAYNGLVVPAHIDSSV
                     NSILSQLGFMPEVPQFQLFGISACLDVKSWVQDNPYFKDKVFLRASDAHYLNDIGKGY
                     SIITVEKPSVQELFLAAKGCGRRKIEI"
     gene            complement(12445..12786)
                     /locus_tag="PLG69_04000"
     CDS             complement(12445..12786)
                     /locus_tag="PLG69_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42706.1"
                     /translation="MIKLSDYVTAINGINCTPSLALEGVEINGAYVCDMLSDVMASAK
                     SGQVWITIMKHLNVIAVASLTGIPAIIFAKGNEPETAVIEKAIEEGILLISSKLPTFE
                     LAGILYQLLYS"
     gene            complement(12809..14086)
                     /locus_tag="PLG69_04005"
     CDS             complement(12809..14086)
                     /locus_tag="PLG69_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPI42707.1"
                     /translation="MTETKYFHALQILADNCTGCTACVRVCPTEAIRVRDHKANIDPY
                     RCVDCGNCVNVCRFHAIIPLSDPLEIIHKFKYKLAIISSSFFGQFTEDISYQVAKQAV
                     LNLGFDQVAEEAAVTDFMINIIRDYIRKNRDKRPILSSNCPSVVRLIQLKYPSLLPNL
                     FHQEAPMSILTRYFRDKISKEYGLEEKEIGIFLIVPCIAHVTAVHQPEGAYKHLQDGA
                     FSIGMIYGKIRDSLKELQNNPPSIETYPQGLTWALSGVHAELVDCEDIRTLSVNGVEN
                     VMEILSRVEDHYLDQYDYIVLRTCTNGCVGGCLNVENPFVAMSRIKKMIKEGQGSDFD
                     TSELYELYQKGEFAVVPLAPRPIMELDKDIKKAIQKMKQINEILTMLPGLDCSACGSP
                     TCYALAEDIVLGKASIDDCVVLLRRHSKDSEEE"
     gene            complement(14099..14617)
                     /locus_tag="PLG69_04010"
     CDS             complement(14099..14617)
                     /locus_tag="PLG69_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42708.1"
                     /translation="MQDSAVAEFEMSEYWYIKPDFEEKVQDFFAQKREPEVVLELDIP
                     GKDFNIAGKGSSELKNLLKRLGVDSEILRRIAVAGYEAEINVAAHSQGGKMISNVFDN
                     LIYMQFMDNGPGISNIQQAMTPGFSTADDLVRELGFGAGLGLPNIQKNSDALHITSAL
                     NSPTLVEIIIFF"
     gene            complement(14589..14936)
                     /locus_tag="PLG69_04015"
     CDS             complement(14589..14936)
                     /locus_tag="PLG69_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPI42709.1"
                     /translation="MTLRDIVELLEAEVLYEGADLNREVPCAFASDLISDILMCTKEP
                     TLLLTGLTNNQVIRLADMIDLIGIVFVRGKKPMQDVIDMAKERGLPLVATNMTLYRSS
                     GILYNAGLRSCRI"
     gene            15227..>15879
                     /locus_tag="PLG69_04020"
     CDS             15227..>15879
                     /locus_tag="PLG69_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPI42710.1"
                     /translation="MKLQYLGHSAFLFSDSQNRTILIDPFLTGNPLSPVKASQVKADY
                     IILTHAHSDHIGDALTIADKRKTTIICVVELASIISKNGYNTHPLQIGGAFQFPFGKV
                     KMVTAFHGSSTSNGLYAGLAAGVVFKLDHKTIYHCGDTGIFGDMKLIGEIDKIDVLLI
                     PIGGNYTMDIDDAVRAVSLIQPELAIPMHYNTFDLIRANPQEFVDKITANGFAGKVL"
BASE COUNT         4811 a         3231 c         2754 g         4983 t
ORIGIN      
        1 cgattggtca ttacagggaa atcatcatca agaccttcca gaaaagtcat ctttgaatgc
       61 aactatcaat tttgtaagta ataaacgcat ttgggaaagc agcattgatg tagaccaaag
      121 tttagctcaa agattaactt ccagtgtttc ctattccaaa ccgattggtt cttcatattt
      181 aaatgccggc agctatttta cgcaggattt gattaatgat accgcttctt tatctttacc
      241 gagtgcgtct tttttcttgt ccagtagacc cgtttctgaa ctttttaatg tatcttccaa
      301 ttcctggcta agcaatttta attatcgcta taatgtttat ttagagcata cagggaattt
      361 aagagaaaag aattattcct taggtgattt tttctgggat aacacaattg atcctgctga
      421 tacaatagga attaggatgc tgaatgaaca tcatttggga ataaaacaaa atgccggaat
      481 ttctcataat tccaatttat tcggatggtt aactttaggg aagaattttg actatacgga
      541 agcgtggatg gatagagata gaaatgatga taaatttgcc agaggttatt cctggactgc
      601 atctgcaaat gccagattta atttgtacgg tttgcggaat tttaaaaatt tccctattaa
      661 ttctattcgg catataatta cacctaatat cggttattcc tatcgcccgg acaccaggaa
      721 gaattctgat ttatacagtt ttggcagcat cggggtaagc aattcccaag aggcatccaa
      781 tctaactttt aatttagata atacttggca gatgaaatac ggcaaaaaag gaaatgaaac
      841 aaaacttaac gaccttttat tttggcgatt gggaactaat gctaatctga aaaaaaagga
      901 taaacccttt tctaacatag cacatacact atatttcaaa ccaggaagtt ttaacctggg
      961 aactctatct tttaatgaag gaaaatataa gcttagcagt ttaaaactta gctattcctc
     1021 acagtttagc gcatctcaag atccctataa aattcagtgg gacaaaatga acttaaggaa
     1081 tcaatatttt tctcagggaa tagttcttag cggttctgct ccttacaacc gatatttttc
     1141 tgctcctaaa aacaagagtt ttgaggattt tgaaaattcc gattctcaaa aatcagtttt
     1201 ggattatatg caagaaactg caggaaccaa tgactggagt ttttccatca gtcaaaatat
     1261 ctatgccgat aaagatattt ttcattccgc aaccagtagt ttgagatcaa gcttaacctg
     1321 taaaatcacg caaaactgga gattatctta caccaattat tataacctga aaacaaatga
     1381 tatgctttct caaacacttt ctttttcccg agacctgcat tgctggaaaa tggatgtaaa
     1441 tatcactttg agaaatgatt actgggatta ccgaatttcc ctgtttaata ctttgttacc
     1501 ggatgcttta aggtttcaaa cccatgacag caaaagatat taaacgcgct atttttttag
     1561 accgagatgg cgttataagt cctgatgatt ttggctattt aagcaatcct gaagaatatc
     1621 atctttatcc ttacacaatt gaagcgttaa agatatttaa gaaactaaat tttttgcttt
     1681 tcgtggttac caatcagtcc ggaattgcca ggggttattt tactattgat gacttggaaa
     1741 aagtgctggc aaaaatgcgt tctttgcttt ccgcaggagg aatagaactt gatggcgttt
     1801 attattcccc ttattataaa gacggaatcg ttccaccata taatattgat cacatagatc
     1861 gcaaaccagg cattggaatg ttcaaaaagg catattccga gtttcatttt caaatagagg
     1921 gttcctatat gattggagac cgcattagtg atcttggttt cggtagaaat gctcacctta
     1981 aaaatatctt gctgttgaca gggaatggag aaaaggactt tataaagatt ctggaaacgg
     2041 atgatttaag accggatttc gtctgcgaga atttattgac tgctgctaaa ctaattaggg
     2101 attataagtt atgaaaattt ttgctcatta cagcgaaacc aaattcaagt cctttacttt
     2161 tgaggaacag ataggagcat tgaataaact tatcttatca ttagaaaaga atttaacgga
     2221 tacggaaaaa cgctctcaga taattgagca tattttagaa ttatatcttt tagtagaaaa
     2281 acccttacct caaaaaatgc agctctttat aaaagaatta aatccgcact taagtcctca
     2341 tcaattatta aataaactct atttttactg tgatggagct atcagaaaag atagtcagat
     2401 ggcacttcaa acggaaaaag gaaaagttat tcttgatcct gatattcgcc aaaaagcaaa
     2461 acagataacc gtgatttgtg ataatttacg gtcggtgttt aatgttggtt ctctttttcg
     2521 gactgcggaa tgcttaggga taggaaaaat tattgtctgc ggaataactc ctactccaga
     2581 acatagtaat atgccaaaaa cggctatggg aacagaaaaa cttgttgcct ggcaatattt
     2641 tccaagcact aatgaagcaa ttgctttttg ccatcaagaa ggttttttta tttatgctct
     2701 ggaaacgata gataacgcaa aaagtgtatt tcagaccgtt tatcaatttc ctcttgcgat
     2761 tgttatcgga aatgaagctc ttggcataga gtcatcaatt ttagagcttt gtgatgaatt
     2821 tatcactttg cctcaattag gttggaaaaa ttgtttaaat gttggcgtag caacaggaat
     2881 tgtcctttat caaattctgt ttggaggttt taatggataa ccttgcttat tttgaaaaac
     2941 tgcttatttt actgcaagaa catagccctg tctggcaggc aaatattatt gaaagcgatg
     3001 gttcaactcc ggcaaaaacg ggaatgaaat tagccattcc gttaaaggaa gaaccatttg
     3061 gcaatttggg tggaggggaa ttggaacata cggttataga gaaaattaga aatgataaac
     3121 ccggaaaagc cacaatttat attttcaatt tatctgcaga tggctctaaa gccggaatag
     3181 caacctctat gctgtgtgga ggtaatgtaa aagtatatgt tgaaccgtta ttttcccccg
     3241 ataaacttta tattattgga gcgggacatt gtggcaaagc attaggaaaa ttagcttccc
     3301 tttgcggttt ttatgttgtc ctgatagaca accgaaaaga aattattgat aatttacctg
     3361 aggattgttg tcatcgtgct atacatcacg attatgcaaa cctggctgag attattgatt
     3421 ttaatcccaa tacttggatt gtgattatga ctcacggtca cttgcacgat aaagaagttc
     3481 tggaacaatg cctgagaaaa ccctttcgct atttaggaat gataggcagt aaaacaaaag
     3541 cagccgctac ttttactaaa ttgaaagaaa agggattcac cccggaagag ctggctaaat
     3601 gccattcacc tgtaggttta tctatcggat caaatacacc ttacgaaatt gcagtgagca
     3661 ttatggcaga acttatctcg ttgcgtaatc ttcaagataa agagtaaaag agtttttttc
     3721 acaaagagat aaagagaaaa agagtttcat tcacaaagag ataaagagta aaagagtttt
     3781 tttacaaaga ggaaaagagt ttttttacat agaggaaaag agtttttttc acaaagagat
     3841 aaagagataa agaggaaaaa agtattattc acaaaaagga aaagagaaat tatcgctgga
     3901 aaagttttga cttacaattt tcacctgcct tcccgaatac actataccac aatatattgc
     3961 tcttacaact ggaactatta ctagtttccc aaattaaaaa attgtaagtt aaaacagaaa
     4021 aatgctcaca tccaaaccaa ttatccccta cttacaaact attcagaaag caattcgttt
     4081 ttatagctac tttcactttt ttcacaaggt ttattgattc taataaatca gtagaaaaga
     4141 taaaaaaagc atatattata atttgattac ctacttcctc ttcagtataa ttataatata
     4201 aagacattta tttctcagag ttctacgaac tctttgtcaa ataggaaatt gcaaacccat
     4261 ttttaaccaa ttgccttgtt tgagattata ctgtgttttt tatcatctga taaaaaaata
     4321 aggcggagaa agatatctcc gcctttctgt atcttttagt agtaggagtt tatccgtgtt
     4381 tatgagtaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctccgcctt
     4501 tctgtatctt ttagtagtag gagtttatcc gtgtttatga gtaatttctg cggtaacctg
     4561 tccgttgaat acagagcgtt taaagtaatg cgtatgcaat agtttaaggg caagtttgga
     4621 atttggttca ccgaggaatt tttcatagac ctttagaact tccggattat gatgcgacat
     4681 tctgacaggt aaattacggt cttcttcata taaagccaaa cctcttctcg ctctggaagc
     4741 aatatcattg ccgtaaggtt ggcctcctcc acctacacaa cctcccggac aagccataat
     4801 ttcaataaaa tgccaggggg attcaccttt tgtggcaaga ccttcacgca cacgatcaaa
     4861 aaccttacgc gcgttggaaa gtccatgagc aaccgctaca ttcacttttc caaagccggg
     4921 aacatcaacc gatgcttctt taactccttc caagccacgc actgccataa tatccacatt
     4981 ctgcaaatcc gtgccggtta caaggtaata agcactacga attgcagctt ccataactcc
     5041 acctgtggct ccaaaaattg ttcctgcacc ggtataaaaa ctcattcctt cgtccggttc
     5101 ttcatccggt atgttagcaa aatcaattcc cgcttcttta atcatccgaa taaattcgcg
     5161 agtagttaaa acataatcgg tatcctgaaa accgctatcg cttaattccg gacgtcgtgc
     5221 ttcaaatttc ttagcggtgc atggcataat ggcaaccgag acaattttgg caggatcaat
     5281 tcctttttcc tgggcataat aggtcttgga aagaactcca aacatactca tcggtgattt
     5341 tgccgttgaa acacaatccg ctaaatcagg ataataggtc tccataaatt taatccaacc
     5401 gggagaacag gaagtaatca gcggtctctt ttctcccgct ttcaatttgg ttacgcattc
     5461 cgtgccttct tccataatgg ttaaatcggc agtaaaattg gtatccataa ccgaatcaaa
     5521 accgatttta cgcagagctg catacatttt tttaggagtt acactgccta aaggcattcc
     5581 aaattcttca cctaaagaaa cacgaatgga aggtgcttcc tgaactatca catgtttttc
     5641 agggtctaaa atagcttccc aaacagcatc cagttcactt ctttcgtgca atgcacccgt
     5701 aggacaataa acagtacact gaccacaatt aacacatggg ctatttgcca ttccctgact
     5761 gaaggcagta tcaatatggg tatcaattcc tctttcagca aaagtgagag cataaaccgt
     5821 ttgcacttcg ttacatacgg ttatacaacg tccgcatccg atacatttat tggtatctct
     5881 gataatggac aaactggatt catcggcagg tgttttaacc aaaatgttgg gtaacgcctc
     5941 agaatccact cccagattat ttgccaggga ttgtaattcg catttattgt tagcaggaca
     6001 agtaggacaa gttacatcat gattggaaag aatcatttcc acaactgtct tacgatagga
     6061 acgaagtttc tttgaattag taacaatttt cattccttcc cccacaggag tacaacaggc
     6121 acgtttggga gttggatttc ctgcaatttc cacaacacaa acaccacaat ttcctgtagg
     6181 aggtaaatcg gggtgataac acaaacgggg aatataaatt ccaattttat ctgcggcttc
     6241 tataatagtc attgtctctg gaacttccag atgatgaccg tctataaaga catttactgt
     6301 tttagccata atatcaatcc tcttattttc ttacttttta gttatttcaa tgaattcatc
     6361 ggtaaaattt tcaatcgcac tgcggacagc cataattggc gattgaccta aagcacagaa
     6421 agatgtttgc tgcatcgttt cagctattaa gagcattaaa ttcacatctt ccatagtccc
     6481 gtttcctttg cggattttgg atattagctg ataaagctgt tgggttccgt ttttacaagg
     6541 agaacatttt ccgcaggatt catggcggaa gaaccgcaga atactccaaa gcaaatcaac
     6601 aatactctgg tgttcattca aaactaaaat ggctccgctc cctaaaacag cagaatattg
     6661 attcaagctg gtaaaatcca ttttcacatc taaaaggcga tcggaaagaa taactccggc
     6721 tgctccacct accaatgctg ctttgaaacg gaaacctttt ttcattccac ctgcataatc
     6781 gttgataatg gttcttaaag ttgttcccat atccacttcg caaagtcccg gataagcaac
     6841 atctccaaga attgtataga ccttagttcc aggacaatca ggggttccat attgcaaaaa
     6901 ttcatcggct cccttggcaa taatgaaagg cacatttgcc aaggtctcaa cattattaac
     6961 tatcgtagga gctttccaaa gtccttccac tccgggaaaa ggtggtttcc aacgcggatt
     7021 tcctctgttt ccttccaggg attctattaa agctgtttcc tctccacaaa cataagctcc
     7081 ggctccaatt ttgagttcaa tatccaagtc aaatccgcta tcaaagatat tttttcctaa
     7141 aatgccatag tcataagctt gtttaatggc tttttccaaa cgggcaatac agagcttata
     7201 ttcaccgcgg atatagatat atgccttact agcacctacc gcacgcgcac aaagtataat
     7261 accttccagc aattgatgcg ggtctccttc cataattaaa cggtctttaa aagtgccggg
     7321 ttctccttca tcagcattaa caactacata tttttgggga acattaagac ctgcggtaaa
     7381 actccattta agtcctgctg gaaatgctgc tccgcctctt cctttcaatc ccgatttttt
     7441 cacttcggca ataatatcat ttggttgcat attagttaat gccttttcca atgctacata
     7501 gccgccagca ccaatatagt catcaatatt ttcggggtca atgatacctg aattgcgtaa
     7561 aacaatgcgg ttttcatagt tgaacttggg actgaatttt tctgtaactc caaccattaa
     7621 gcgttttacc gggcgtcctt tcaggaaatg ttcctgcaca agttccggaa tatcttctat
     7681 tttaacgcct tcataattga tgttttcggg atatacagtc aagcaaatcc ctctgccgaa
     7741 aaagcccaaa ggtcctgttt ccagaatatt gatttcttcc gctaaatcat ttttggctaa
     7801 ttcattgcgg agagcagtaa taaattcctg aactccagct gcgagagagg tctcattaat
     7861 tgagaccaaa atatgactgc gatagaattt cattatctcc tccctctgat tctttcaata
     7921 atttcttcca ctttctcttc cgtgaggtca ccgtaaacat catcattaat catcatcaca
     7981 ggtgcatttc cacaaacacc taaacaggaa gtaaattcaa gtgtgaataa gccgtctttc
     8041 gttgtttcgc caatattgat gccaagcaga actttcagtt tccgcagcat attgtccgag
     8101 cccttaatat aacagggtgg tgattcgcaa aggcggataa tatttttgcc tcttggttta
     8161 gtgctataca tagtgtaaaa tgtcagcact ccataaatat gattaacagg aatatcaaga
     8221 tattcagcaa cagtatctac tgcttcagga gaaatatagt gctgtgggtc ttgatcctga
     8281 atttcatgga gaatctgaat cagattatcc ttacggggag catatttttg acaaatttct
     8341 tttatcatag ctcttatcct ttattcttct tgattttgct tttgtaattc ctctgcttca
     8401 cgcattgctt taacctcagg atgcattccc ggaattttgg aaatggcttt tacagggcat
     8461 acatccatac aggttccgca cttgatgcag ggtagttgat tgatcgtata tttttcttcc
     8521 cgactgccgg aaatgcaact tacaggacat ttgcgcgcac ataaggaaca tcctatgcat
     8581 ttatccttgt ctatctcaaa atgcattaaa tcgggacaaa ccttattggc gcaactttta
     8641 tcccgaatat gagcttcata ttcatcgcgg aaataacgaa ttgtggaaag aacaggattg
     8701 ggagctgttt gtcctaatcc acaaagggaa atcttggaaa tggtttctcc cagctgttga
     8761 agttcttcaa tatccccttc tttgccatat ccgctgtcaa tcctttttaa tatttccagc
     8821 atttgtttca agccaatctt gcagggagaa cattttccac aggattcatc cacgcagaaa
     8881 tccataaaga acttggcaac attcaccata cacttttctt cattcattac aatcactccc
     8941 cccgaaccca tcatcgctcc acgttctttt aagctttcat aatcaatagg aacatccaaa
     9001 tgctgagtag ttaaacagcc gccactggga cctcctaatt gaactgattt gaatttgtgg
     9061 tcgccaatca ttcctccacc tacatcaaaa atcagttccc gtaaagtaat tcccattgga
     9121 acttcaacca aaccggtatt tcttatatct cctgttaaag caaagacctt tgtcccttta
     9181 ctgttttctg ttcccataga ggcaaaccaa tcagctcctt ttaggattat agttgcaata
     9241 ttgcctaagg tctctacatt atttactacg gttggctttt tataaagacc ttgcactgca
     9301 ggatagggtg gtttgggagt tggatttcct ctttgacctt caatggattg aattaaagct
     9361 gtctcttcgc cacaaacaaa ggcacctgca cccgttctaa cttcagcttc aaaactgaat
     9421 tcactgccaa agatgttttt acctaacaaa ccggtttcct tagctttttc aatagccatt
     9481 ttgatccgct tgatagctaa aggatattct gcccggatat aaaaatatcc gttttgggct
     9541 ccggtagcat aagcagcaat catcattcct tccaaaacac gatgcggatc accttccagt
     9601 aaacttctat ccataaaagc accgggatcg ccttcgtctc cattgcaaat aaagtatttt
     9661 tcctcggaag gattatcgtg caccatttgc catttaaggt aagtaggaaa accgcctcct
     9721 ccccttcccc gcaatcctga ttttttaata atatctatgg tctgctgagg agttagagta
     9781 gttaaaacag ttcccaatgc ttcataacca ccggtagcaa tgtattcttc aatgctttcc
     9841 ggatcaatat aaccgcaatt ggcaagggct actttcacct gtttttggta aaaagggatt
     9901 tctttctgcg taaggaaggt cttatcttct tcatcctgaa gcatcaaacg ctttacagga
     9961 cgacccttta aaaagtgttc attcacaatt tcttctacat catcaggggt aacttttttg
    10021 tagaaaacac cttccgggta aattacaatt accggaccat aatcacaggg tcccatacaa
    10081 ccggtttcaa tgatgttgat ttcatttgtc agtccttttg ttttcaggac attatgaaac
    10141 tgttctttaa cttttaaggc accggcactt acacatcctg acccacagca aatcaatagg
    10201 tcaatacgtt ttaatgacat tcttttcctc cagaatcagt tgcctgtagc gacaacataa
    10261 tctgaaacga ttctgccatt tactatgtgt tctacaacta cctttttaac tttttcggga
    10321 gtcatattgc cgtaagtgac ttttggcttg ccttcttcaa taatatccac aacgggttcc
    10381 agggaagaaa gccctttttc acctgtttga gtaactaaaa catcagttaa attgcggtta
    10441 gcaatttctt ccaggaaggt tttcataact tctcttgcgc ccatagcgat tccggatgtg
    10501 cccatcccga ccactatttt gatgcgtgtg tttccaccgc gaagtttcat ttcttcctga
    10561 gctttttcac ggattttttt aaggtctgcg agtgttttca tcagacatcc tccatatttg
    10621 tgttttgtat tccttctaat aatgattgat caatgtattc aataacatcg ggataagtta
    10681 aaggtatatt tcccaattct tctttgatag cggcggtatc aaaataaaag cttttttcca
    10741 caccgcgttc gtggctgata agtttaatgg taaaatcaac ttcgggatgg cctataatag
    10801 cacctaaaag agtatctttc aagtttccca ggggcattct gtctatatgg tctaattgaa
    10861 aatccaccgt taaaacagtg ccaaaaccag gttcactggt cattttaaaa ctgccgtttg
    10921 ttgcttctgc attttgttta accaggggta ttccgagccc tacttttttc tcccgctctt
    10981 ctttagatgt ataaaatgga ctttgcgcct tagctaaggt ttgagaatcc attcctactc
    11041 catcgtcttc cacaattaaa cgcagaatat tatcctgcac atcgcgaata atacggattt
    11101 taatgttctt tgctttggcg cgaacggaat tttctataat atccagaagg tgtaatgaaa
    11161 tatcttgcat ttcagaatcc ggattatttg tagcgtttca ggatttcggc tattttatcc
    11221 ttactatcaa ttctaccgta ggtttcttcc ccgattacaa ataccggagc taaagaacag
    11281 gcacctacac aacgaactgc actcattgta aaaatgccgt cttttgtggt ctcccccatt
    11341 tggattccca attcttcaga aagcatttgg actaagcgag gagctccttt aacgaaacag
    11401 gcagtgccta aacaaacatt aagattatat tttccgcgag gagtcatcgt aaaaaagttg
    11461 taaaaagtaa ctactccata aatctgattg acgggaatat ttaattcctg agctataaat
    11521 tcttgcactt ccacaggcag gtaaccgaat atttcctgag ctgaacgaag aatttcaatg
    11581 aggggattgc ggattttttt cttttcttca atcacttttt tcagatgtgc atattggtca
    11641 ttttgcatct gatctgcggg aatcataatt tctccttatt ttatctatat ttttattaaa
    11701 tttcaatttt acgcctgcca caaccctttg cagccaaaaa caattcctgc accgatggct
    11761 tttctacagt tattatgctg tagcccttgc caatatcatt taagtaatga gcatccgagg
    11821 cacgcaaaaa gaccttatcc ttaaaataag gattatcctg cacccagctt ttaacatcca
    11881 gacaagcgct tatcccgaaa agctgaaatt gaggcacttc tggcataaaa cccagctgac
    11941 ttaagatact atttacactg gaatcaatat gagccggaac aaccagtcca ttatatgcct
    12001 gaaccttttc cactacagtg tttaagttcc actgtgagga atttattaga gcgcgtggtt
    12061 ccactcttaa aatgttctca ttttcgtcaa tgattacttg atccccgaaa aaatcgggat
    12121 cgttatcaag gggaaacaag ctttcataga gttctgtatt gaatttttct gcggaagaag
    12181 aatcctcaaa ataggcaagg agatgaattt cttccacact ttgaatttct actccccaag
    12241 tgaaaaaaag tccttctttt tccgctactt tttggtaggc aggacaattt gccatactgt
    12301 tatgatcagt aattgccagc cattgaatgc caaattgctt taatctttgc ataattgaat
    12361 tgggagacat ctccaaaccc ccacaaggag ataaaacaga atgaatatgt aaatcagcgc
    12421 ggagcgattg catccgaaaa taacttagga ataaagtaat tggtaaagaa taccagcaag
    12481 ttcaaaagta ggtaacttgc tggaaataag taaaatgcct tcctcaatcg ctttttctat
    12541 aactgcagtt tcgggttcat ttcctttagc aaaaattatt gccggaatgc cagtcaaaga
    12601 agcaacagca ataacattca gatgtttcat aattgttatc caaacctgtc ctgatttggc
    12661 agaagccata acatcgctta gcatatcaca aacataggca ccgtttattt ccactccttc
    12721 cagggctaga gaaggagtgc agttaatgcc attaatagca gtaacataat cacttagctt
    12781 aatcatttat cctatcctta ttttgatttt attcttcctc actatcttta ctatgacgcc
    12841 ttaacagcac cacacaatca tcaatggatg cctttcctaa aacgatatct tctgccagag
    12901 cataacaggt agggcttcca caggcactgc aatcaagtcc tggaagcatt gtgaggattt
    12961 catttatttg tttcattttc tgaattgctt ttttgatatc cttatccagt tccataattg
    13021 gtctgggggc aagtggaact acggcaaatt cgcctttttg atagagttca taaagttcac
    13081 tggtatcaaa atcgcttccc tgaccttctt taatcatttt tttaattctg ctcatagcta
    13141 cgaaaggatt ttccacattt aaacaaccac caacacaacc attggtacaa gttcgcaata
    13201 ctatatagtc atattgatca aggtaatgat cttccacccg ggaaaggatt tccatcacat
    13261 tttccactcc gtttactgat aaagtgcgga tatcttcaca atcaacaagt tctgcatgca
    13321 ctccggaaag agcccaagtt aaaccttggg gatatgtttc aatagaagga ggattatttt
    13381 gcagttcttt taaggagtcc cgtattttgc cataaatcat tccaatggaa aaagctccat
    13441 cctgaagatg tttataagcg ccttcaggtt ggtgaactgc agtcacatga gcaatacaag
    13501 gaacaattaa aaagatgcct atttcttttt cttccaaacc gtattctttg cttatcttat
    13561 ctctaaaata cctggttaaa atgctcattg gagcttcctg gtgaaaaaga ttcggcagta
    13621 aagaaggata ttttagttga attaaacgca caacggatgg acaattacta cttaaaatag
    13681 gtcttttatc ccgatttttt ctgatataat cacgaataat gttaatcata aaatccgtta
    13741 cagccgcttc ttctgccact tgatcaaaac cgagatttag aactgcctgt ttggcaactt
    13801 gatagctaat atcttccgta aattggccaa aaaaagaaga agaaataata gccagcttgt
    13861 atttgaactt atgaattatt tccagaggat cgctaagcgg aattatagca tgaaaacgac
    13921 aaacatttac acaattgcca caatccacac aacggtaagg atcaatattt gccttgtggt
    13981 ctctaaccct tattgcttct gtaggacaaa ctctaacgca agcagtgcag ccggtacaat
    14041 tatctgccaa aatttgcaga gcatgaaaat atttcgtttc agtcatatcg cttttatctt
    14101 agaaaaatat tattatttct actaaggtgg gagaatttaa tgccgatgtt atgtgcaatg
    14161 catcactgtt tttttgaata ttaggtaagc ccaaaccggc tccaaaacct aattcgcgca
    14221 ctaaatcatc ggctgtagaa aaaccggggg tcattgcttg ttgaatatta ctaattccag
    14281 gtccgttatc cataaactgc atataaatta gattatcaaa cacattgctg atcatttttc
    14341 caccttgaga atgagcagca acatttattt ctgcctcata acccgcaact gcaatgcgtc
    14401 ttaaaatttc cgaatctact cctaagcgtt ttagcaaatt cttcaattcc gaggaaccct
    14461 ttccggcaat attaaaatcc ttccctggaa tatcaagttc caaaactact tccggttctc
    14521 ttttttgagc aaaaaagtcc tgcacttttt cttcaaaatc tggttttata taccagtatt
    14581 cggacatttc aaattcggca actgcggagt cctgcattat ataatatccc acttgaacgg
    14641 tataaagtca tattagtcgc aactaatgga agacctcgtt cttttgccat atcaataaca
    14701 tcttgcattg gttttttacc acgaacgaaa acaatgccta ttaaatctat catatcggca
    14761 agacggatta cctgattatt ggtaagtcca gttaaaagta gggttggctc cttggtgcac
    14821 atcagaatat cggaaattaa atccgaggca aaagcacagg gaacttcccg attcaaatca
    14881 gctccttcgt aaagaacttc cgcttccaga agttctacaa tatctcgtaa ggtcattcac
    14941 tttttccctt aatctttttt ttgttatttc attaaatata gatatagaaa ttcagtcaag
    15001 ggaaaagtaa gagggtagag aggttttaac ttacaatttt cactttccct cccaataatc
    15061 attaccccac caactattac acatacaacc aagctataac tggttcataa attaaaaaat
    15121 tgtaagttaa aacaaaatct attgtcgctt ccccctatat tttaatgttg tttattgtgc
    15181 ccaaacgaat tattatatct ttagcggaaa caaaaggagg agaaaaatga agcttcaata
    15241 tttgggacat tcggcttttc tgttttcaga tagccaaaat cgcacaatcc ttatagaccc
    15301 ttttttaaca ggcaatcccc tgtcacctgt aaaagccagt caagtgaaag cagattatat
    15361 cattcttact catgcccatt ccgatcatat aggtgatgca ttaaccatag cagataagcg
    15421 aaaaaccaca attatctgtg tagtagagct tgcttccata atttccaaaa atggctataa
    15481 tactcatcct ctacaaatag gaggtgcttt tcaatttcct ttcggtaaag taaaaatggt
    15541 tactgctttt catggttcct caacctctaa cggtctttat gctggtttag ctgcaggagt
    15601 tgttttcaaa ttggaccata aaaccattta tcattgtggc gatacaggca tttttggaga
    15661 tatgaaattg attggagaga tagataaaat tgatgttctc ctgattccta taggtggcaa
    15721 ttatacaatg gatattgatg atgctgtgcg tgccgtcagt ttgattcagc cggaattggc
    15781 tattcctatg cactataata cttttgattt aattagagct aatccgcagg aattcgtgga
    15841 taaaattact gctaacggtt ttgcaggaaa ggtgttaaa
//
LOCUS       DAOPFL010000035        15608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_4311_length_15608_cov_99.690799, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000035 DAOPFL010000000
VERSION     DAOPFL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15608
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4311_length_15608_cov_99.690799"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PLG69_04025"
     CDS             complement(<1..1072)
                     /locus_tag="PLG69_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPI42711.1"
                     /translation="MNKKWLIPSPPGKEINPEIEKLSAEFKLPRLVAELLYRKGIKTV
                     NSAQEFFKPSMDNLYDPYLFPDMEKAVQRILRAIDSKEKITIYGDYDVDGTTATALLY
                     LGLKRIGANIDFYIPHRMIDGYGLSLGSLDALRENGSSLIISVDCGVNAIEEINAINS
                     MGMEIIITDHHNPKDELPPAFAIINPKLPGTKYPYEHLAGVGVAYKLLMAIYNKLGID
                     SAENKLKYMDLVAVGTIADIVPLTSENRIFASIGLQHLIEKKNLGLNALVQISGLNQK
                     NLDTTDIVFGIAPRINAAGRMGSASVSVELLISTDEAKSMELAEIIEHQNSLRQQEDQ
                     KTFQEACDIIEKKYKDLQQTSCM"
     gene            1493..2392
                     /locus_tag="PLG69_04030"
     CDS             1493..2392
                     /locus_tag="PLG69_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPI42712.1"
                     /translation="MLLASFLEAHNILFENRVLTKEQVYKKLVDRLCSQYKLPVSGSK
                     LLELILHREEEVSSAYPTGIAIPHIRMDGFNDTLIAMAFLENPLDYNGIKVNWVVLIL
                     TDKTSSKTYLNIVAALLKLSKDKVAMKALASAGDGQSVIHYLKKNEVEVKKDVIIADI
                     MVQNPIAVLPQNSLRELINLMSTHKVAGMPVVDETGKYVGEVNVLNLLEVGIPNYVMM
                     LDNLSFLSSYEPLENLFSQQDLLFVKDIMTTDEIFVRPEASIIETVFLMVSHNKRYLS
                     VVKEGKLVGLITAMDILRKVIAV"
     gene            2397..3674
                     /locus_tag="PLG69_04035"
     CDS             2397..3674
                     /locus_tag="PLG69_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HPI42713.1"
                     /translation="MLLMIIALLVFIVTYLCIITEWVNKMLAALIGGFVIIVLGVVDQ
                     SIAFSAIDWNVIFFLIGMMLTISVMRETGMFMYIAIKTAKIAKGSPLKIMAMMFIATA
                     VISAILGSVTTVMILVPIVLLIAEELKITPAPFIITMVVASNMGGAATMIGDPPNILI
                     ASATKYTFIDFFLNLTPAIVIIVIGSLGLIWLLYRGKMYVSNERRAKIMEYNDKNLIT
                     HPKLLWITLTVVGLMLLAFIFQKPLHLENATIAMAAGLILVFIGSRKKVETIILNDID
                     WITIFFFIGLFMIVEGLVHTGFIDLLANGVMSITNGEPKTTSMVILWLSGILSAWIDN
                     IPFVAAMIPMIKSIGLHMQSAVQVQPLWWALSLGTCLGGNGTLVGASANIVAVGIANR
                     NGYKISFMDYTKIGILFALESMLLSSVYIWFRY"
     gene            complement(3997..7518)
                     /gene="nifJ"
                     /locus_tag="PLG69_04040"
     CDS             complement(3997..7518)
                     /gene="nifJ"
                     /locus_tag="PLG69_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPI42714.1"
                     /translation="MAKQTKATMDGNTAAAHIAYAFAEVAAIYPITPSSTMGELVDAW
                     ASEGRKNINNTKVDVIEMQSEAGAAGAVHGSLSAGAVTTTFTASQGLMLMLPNMHKIA
                     GEMLPTVFYVSARSLAAQSLSIFGDHSDVMSARNTGFALIACNSVQEVMDLGLVAQLA
                     TMKTRIPFLVFFDGFRTSHELQKIDVIDYETIAELNDPSLVEAFRKNALSPDKPRMKV
                     GQEAPDVYFQGRETSNLYYAKAPAIIAETMEQVAEKTGRHYQLFEYVGSPDAEDIIVA
                     MGSSCETIQETIEWLNAKRGMKLGLIKVHLYRPFCVQSFLSAIPASVKRIAVLDRTKE
                     PGSIGEPLYLDVVSALKNRPELYIIGGRYGLSSKEFTPSMVLAVYKHLAKNGFHGFTV
                     GIEDDVSHLSLPLEETINTVPEGTISCKFWGLGSDGTVGANKNSIKIIGDHTDMYVQG
                     YFQYDSKKSGGITRSHLRFGKTPIHSQYLVAQEDFVACHNQAFIGRFDLLGGIKENGV
                     FLLNSNWSRDEAFYNLTRPMQETIINKKIKFYNIDGLKIAEEVGLGGRINTTMQTAFF
                     LISGVLERNEAIALIKDSIRKTYARKGEKVVQMNLEAVDKVNEALVQIEVPETLPDKY
                     MPMKKLVPEDADDFILNVIEPIMREQGDQVKVSQMPLDGYVETGTAKLEKRKVAPSVP
                     HWLPENCIQCNQCSFVCPHSAIRAKLIKEEDLANKPASFNTLKAIGAPGYEYKVQVYI
                     DDCQSCKVCVNECPKSALVMSPIEEERTKGEQVNYEYFETLPNDVLANFKETTVKGSQ
                     FKQPLLEFSGACAGCGETPYVKLLTQLFGDRMIIANATGCSSIWGGTFPTVPYCKNKD
                     GKGPAWANSLFEDNAEYGFGMRLAIRSHRKLLQKAMQELMDKNINEELKSAMQYSLDN
                     WDKVNEETKANADKIRALLPKAMETACEGCKILLKKVQELQDYLIEKSIWSIGGDGWA
                     YDIGFGGLDHTLALNQNLNILVLDTEVYSNTGGQASKSTPLGSIARFAEAGKNTNKKD
                     LGMMLMSYGYVYVASIAMGANKNQALQAIKEAEAYPGPSIVIAYAPCINHGFDMSLSQ
                     KHQKLAVDAGYWLLYRYNPLNKQEGKNPLTLDSKEPTVSVQEFLDSENRYAGLKNIFP
                     ERAKELNAGAAVFFKERYETYKKLAEG"
     gene            7991..8233
                     /gene="xseB"
                     /locus_tag="PLG69_04045"
     CDS             7991..8233
                     /gene="xseB"
                     /locus_tag="PLG69_04045"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPI42715.1"
                     /translation="MSVDQEKINAMSFEESLKELEKIVDRLSTGETNLDELLSLYEAG
                     VAYLKHCQSRLSSAEAKIKILSEQLSSQQVTEGNNG"
     gene            8226..9116
                     /locus_tag="PLG69_04050"
     CDS             8226..9116
                     /locus_tag="PLG69_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPI42716.1"
                     /translation="MDRKIFLKKDMKEKQELVNIILDRFLPRKDEYPKDLHKAMRYSV
                     FAGGKRLRPYLTMTAFEMFNSNIELITPVAAGIEMIHTYSLIHDDLPDIDNDDFRRGK
                     KSCHTIFGEGVALLAGDALLLSAFELVTLAELDAKLRVQFVHELAKEVGIKGLIAGQM
                     VDIESEGKKVDKKTLHYIHENKTARLINLSLRFGALAGEAKEEELKILDEYGKLIGLV
                     FQIVDDLLDIEGTQEELGKTIGKDAESAKATFPAVYGIAESHKKAEELTEKAREILAP
                     LGEKALKLDILAEYLLGRKE"
     gene            9113..9547
                     /gene="dut"
                     /locus_tag="PLG69_04055"
     CDS             9113..9547
                     /gene="dut"
                     /locus_tag="PLG69_04055"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPI42717.1"
                     /translation="MNILVQKLSPTATIPQKMTENASGFDLFADIGEPIILNPLEWRQ
                     ISTGIALEIPVGYEAQIRPRSGLSSTLGLSILNSPGTIDSDYRGEIKVILINLSPNPI
                     RIEPEMRIAQIVFCPVISPQLIEAQTLSESLRQDGGFGHTGQ"
     gene            9550..10461
                     /gene="nadA"
                     /locus_tag="PLG69_04060"
     CDS             9550..10461
                     /gene="nadA"
                     /locus_tag="PLG69_04060"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPI42718.1"
                     /translation="MKNNELIQEIKHLKKEKNALILAHNYQIVEIQDLADYRGDSLQL
                     SILAKEVKAPLIVFCGVKFMAETAAILNPESTILLPAIGAGCPMADMISLSQLKAFKQ
                     NYPGSPVVCYVNSSVEVKAESDVCCTSSNAVKILKSFSDEKPILFVPDRNLGSWAGKQ
                     SGKKVITWDGYCLVHQYGFSEEDVLTLKNEYPDYKLLVHPECPPQIIKYADLVISTGG
                     MVDWVKNNDKAIIATEIGLTEYLQHIYPEKSLIPLSSKAICKNMKKTTLENVYNALKY
                     NQYVISIEPNTAQKARKAIDRMLELSR"
     gene            10511..11389
                     /locus_tag="PLG69_04065"
     CDS             10511..11389
                     /locus_tag="PLG69_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42719.1"
                     /translation="MKTRFFLILLTLIILFGVWLYLSYNLGTNEKDKLEKLANLPIYA
                     YVADTTKVAPILTELKTVPGIKNVVHETALQAATELIQSYGLPLNEEMIKDYILPDII
                     TINLQPNRVSISNKPVIIDILRSHIPETDIDSQSSAYSLIIQELSNLDLYNIVFNVFI
                     AVLILLIFVFSRTALELNTLLHYKGIKYSALEKIRHQRQGLQHTLLMLIVPLPLCLLA
                     YFAYVYFKPLPQVIPYWVFLVQAGTVILGTMINYFILHSFEQQVALQENPIEVITPPE
                     MESNGNKEQENDTPFA"
     gene            11370..11933
                     /gene="pgsA"
                     /locus_tag="PLG69_04070"
     CDS             11370..11933
                     /gene="pgsA"
                     /locus_tag="PLG69_04070"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPI42720.1"
                     /translation="MTRHLPNILTVFRIILVPVFLYFLFLAKLPANILIALIIFIVAC
                     FTDYLDGMLARKMNIISDFGKLMDPLADKLIVLSALAGLCWLPPFQVSIVVFFIIFLR
                     ELVITILREIYQKKGIVVPADFFGKLKTVLQMAGIIVAFAFWVFINPVPPLVILIINV
                     WFWFVALITVLSGLNYIITIAKRSNYA"
     gene            11926..12987
                     /locus_tag="PLG69_04075"
     CDS             11926..12987
                     /locus_tag="PLG69_04075"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPI42721.1"
                     /translation="MRKYMIFLLLLLVLFACSKEGGKIDRYTIYDKVIDHSKPLAMGD
                     DRDVYVFCDSLDWQGLKPFIGSAIEQQIIIVYPEQYFNLMMADIKDVKRLSKYKNLVF
                     IGHLESDLPVSAYMKGVLSQDFITRVQQTGGELFTSENLNCCDQIVLFLLGKDAKSLQ
                     KIGALQAENIFSLLLKRFTNRQGYYAYQGKVIDPSFFDNYPFSLKIPDTYRLYSNDKV
                     GRFLCFLYRARLENREIPDKYISVYYEPMPENKVDLNWLIAKRQEIGKKYFEGDEFDP
                     ELVRKEPFTFKKHPGYRLLGAWKNMKYAIGGGFQSYGFWDEKTKTAYIVDSSVYFPAG
                     DKLPVLVELYVISNSLEIK"
     gene            12984..14729
                     /locus_tag="PLG69_04080"
     CDS             12984..14729
                     /locus_tag="PLG69_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42722.1"
                     /translation="MKTILSCKQLLLWLAFFLLLLSACAGPQTTAQRTIIDNSQPNIP
                     SLYYYISASLKHYEGDFLTADNYYHLALEQDFRSHQIKKQILINSAYAFLSKQQDAEI
                     TVRQFDQARKEMIFDNDLLNAAYSVYSNANNEEGLAWVVSESVLYYPSTLSFLRKFYL
                     DYSKNKKSDENLLELALKYADNNPEDIILTAKMYTLVNPKRSVSLLQEAYELEPKKET
                     DNLLSEITLQYLGKEEALKKFRTYSYPEDKDRMLYFLQMANRNRRLEVVNTLSPILLN
                     TGDSSLIGELAFSAYLEDKTDILQEISRFLLTKDADPLTDSKVVSFLFAEALFSEKLP
                     EPSLYLDYFNSAQDVQDVILYAMLKRSMQAQSLEKETNIIFSEEFVSAVEKRLPENVF
                     SRYLKIASQIKSVEDEDFLQARAELCSYFLQKDLGELEDWTFLLQYYQNEGREEEKIA
                     TLRKAVHKFPDNPLFLNDLGYSLLDYPQFIVEGGFLIQRAVALDPTNPYYQDSLAWFY
                     YLTADFSKAMEHITIPMQMKNAPGEISYHIGMILLANQQKEEAIKYFQSALEDEQTPI
                     YQEKAKKALNELKTD"
BASE COUNT         4746 a         2880 c         3015 g         4967 t
ORIGIN      
        1 ccatacagga tgtttgttgt aagtccttat atttcttttc aataatgtca catgcttctt
       61 gaaatgtttt ctggtcttcc tgttgccgta aagaattctg atgttcaata atttctgcta
      121 gttccatact ctttgcttca tcggttgaaa ttaaaagttc tacggaaacg gaagcgcttc
      181 ccattctgcc ggcagcattt attctgggtg caatgccaaa aacgatatct gtggtatcaa
      241 ggtttttctg attcaacccg gaaatttgga ctaaagcatt taagcccaag ttctttttct
      301 ctattaagtg ctgaagccca atggaagcaa aaatacggtt ttccgaagtg aggggaacga
      361 tatcggcaat tgtgccaact gctacaagat ccatatactt aagcttgttt tccgcactgt
      421 caatacccaa cttattgtag attgccatca gcagtttata ggcaactcca actccggcaa
      481 gatgttcata agggtatttt gttcctggga gtttgggatt gataatagca aaagcaggag
      541 gcaattcatc tttgggattg tgatgatcag taataataat ttccattccc atagagttta
      601 tagcattgat ttcttcaatg gcattcactc cacaatcaac actgataata agagaagaac
      661 cattctctct taaagcatcc aaactgccaa gcgataaacc gtaaccatct atcatccgat
      721 gaggaatgta aaaatcaata ttggcaccga tgcgtttcaa acccaaatac aataaagcgg
      781 tagcggttgt gccatctaca tcgtaatcac cgtaaatagt tattttttct ttgctgtcta
      841 tagcgcgtaa aatacgctga actgcctttt ccatatcggg aaagagatag gggtcatata
      901 aattatccat tgagggctta aaaaattcct gcgcagaatt tactgtcttt atccctttac
      961 ggtataataa ttcagctacc aaacgcggaa gtttgaattc ggcacttaac ttttcaattt
     1021 cagggtttat ttccttacct ggtggagatg gtattaacca ttttttgttc atcattctgc
     1081 ctttatatat ttatttattt tctctattta gatgtggaac taacataacc ttaataggtt
     1141 acagcatatt cttaagagaa ctgtatgctt tatacattct aaactctata attatagagc
     1201 gctaaatcct gtaaagcatt attttcattc cggaaatatg ctgtcttttt agatgaataa
     1261 cttaataaat tgaaaagtgt aaaggtgtaa aaggggaaag gtgaaatacg agttaaacat
     1321 agataacaag caaaaaatct gtatgaagtc gtattccaaa gctgtttcag cagatcatac
     1381 tccattttcc atttattcca tctcacacac tcaccatctt gtcaaaataa atattgaccg
     1441 aaaaagagag agcattaaaa aggaaacaaa attacctgtt aagaggtgtt aaatgttatt
     1501 agccagcttt ctggaagcgc ataatattct gtttgaaaac agggttttaa caaaagaaca
     1561 ggtctataaa aaattggtag accgattgtg cagtcaatat aaactgccag ttagcggttc
     1621 taaactgtta gagctaattt tgcacagaga ggaagaagta agctctgcct atccaacagg
     1681 tattgctatt cctcatattc gtatggatgg ctttaatgat actttgatag caatggcttt
     1741 tttggagaat ccccttgatt ataatggaat aaaagttaac tgggtagttc ttatattaac
     1801 cgataaaaca agttccaaga cctacctgaa tattgttgct gccttgttga aactttccaa
     1861 agataaagta gcaatgaaag ctttggcatc cgcaggagat ggacaaagcg ttattcatta
     1921 tttgaaaaag aatgaagtgg aagtgaaaaa agatgtaatt attgctgata taatggtgca
     1981 aaatccaatt gcagttttgc cccaaaacag tttgcgggaa ctaatcaatc tgatgagtac
     2041 acataaagtt gcaggaatgc ctgtagttga tgaaacagga aaatatgtag gtgaagtgaa
     2101 tgttcttaat ttgttggaag taggaattcc gaattatgta atgatgttgg ataatttaag
     2161 ttttctttct tcttatgaac cactggaaaa tcttttcagc caacaggatt tgctttttgt
     2221 gaaagatatt atgactacag acgaaatatt tgtgcgtcct gaggcatcta taattgaaac
     2281 tgtgttttta atggttagcc ataataagcg ttatctttct gtagtgaagg agggaaaact
     2341 tgtaggtctg attacggcaa tggatatact aagaaaggtt atagcggtat aataaaatgc
     2401 tgttaatgat tattgcttta ttggtcttta ttgtaactta tctatgcatc attacggagt
     2461 gggtcaataa aatgctcgct gctctgatag gtggttttgt aattattgtt ttaggagttg
     2521 tagatcagtc cattgctttt tccgcaattg actggaatgt aatctttttc ctgattggaa
     2581 tgatgcttac catttccgtg atgcgagaaa cgggaatgtt tatgtatatt gctattaaaa
     2641 cggcaaaaat tgccaagggt agtcccttga aaattatggc aatgatgttt atagcaacgg
     2701 cggttatttc tgccatttta ggtagcgtta ccactgtaat gattttggtt ccaatcgttt
     2761 tgctgattgc ggaagaactg aaaataactc ctgcaccttt tattataact atggttgttg
     2821 cttccaatat gggtggagca gcaacgatga ttggtgaccc gccaaatatt cttattgcca
     2881 gtgctactaa atatactttc attgattttt tcctgaatct aactcctgcg attgtgatta
     2941 ttgtaattgg cagtttaggt ttaatatggc ttttatacag agggaaaatg tatgttagca
     3001 atgaaaggcg tgccaaaata atggaatata acgataagaa cctgataact cacccgaaac
     3061 tattgtggat tactcttaca gtagttggtt tgatgttgct ggcatttatt tttcaaaagc
     3121 cgttacattt ggaaaatgct acaattgcaa tggcagcagg tcttattttg gtatttatag
     3181 gtagtcgtaa aaaggtggaa acaatcattc tcaatgatat tgattggatt accatctttt
     3241 tcttcattgg cctttttatg attgtagagg gtttggtgca tactggcttt attgatctct
     3301 tagctaatgg tgtgatgtct atcaccaatg gagagccaaa aacaacttct atggtcattc
     3361 tttggctttc gggcattctt tctgcctgga tagataacat tccctttgta gcagcaatga
     3421 ttccgatgat taaaagtatt ggcttacata tgcaaagtgc agttcaagtt caacctcttt
     3481 ggtgggcttt atctttagga acctgtttag ggggaaatgg aactttagtt ggtgcttctg
     3541 ctaatattgt tgctgtagga atagctaatc gtaatggata taaaatatcc tttatggatt
     3601 atactaagat tgggattctt tttgctctgg agtcaatgct tctctcgtct gtatatattt
     3661 ggttcagata ttaatcctgg aaaagttcga ggattatggc tctcacagat agcactgata
     3721 acacagatta aattttatag agatttttta caggactgac ggcttggttt acgcagattt
     3781 tattataata tcatttcttg aatgatattt cccttgtcct tcttgagaaa gcaggaatat
     3841 agggtatttt tccaaatatc aatgtatttt caatatagga actatataaa gggtattttc
     3901 gggtcgtcac gagtcatata cgccgacaaa gttatcgtct gggacaagtt tgtataggcg
     3961 taaatgactc ctgacttacc atagttgacc gggaggtcaa ccttccgcaa gctttttata
     4021 tgtttcgtat ctttccttaa agaacacagc agcgcctgca ttcaattctt tagctctttc
     4081 cggaaagatg tttttgagtc cggcatagcg attttcggaa tccagaaatt cctgcactga
     4141 aaccgttggc tctttactat ccagggttag aggattttta ccttcctgtt tatttaacgg
     4201 attataacga tagagaagcc aataacctgc atccacagcc aatttttgat gcttttggct
     4261 caaactcata tcaaagccat gattgatgca aggagcataa gcaattacaa ttgagggacc
     4321 cggatatgcc tctgcttctt taattgcctg cagtgcctga tttttattgg cacccatagc
     4381 aatggaagca acataaacat aaccatagct cattagcatc attccgaggt cttttttgtt
     4441 ggtatttttt ccggcttccg caaaccgcgc aatggatcct aagggagttg atttacttgc
     4501 ctgtcctcct gtattggaat agacctcggt atccaaaaca aggatattga ggttctgatt
     4561 tagagccaaa gtatgatcca atcctccaaa accgatatca taagcccaac cgtcaccacc
     4621 aattgaccaa attgatttct caatgaggta atcctgaagt tcctgaacct tttttaggag
     4681 gattttacag ccctcacagg cagtttccat tgcttttgga agcagagctc taattttatc
     4741 ggcattggct tttgtttctt catttacttt atcccaatta tctaaggaat attgcattgc
     4801 ggattttagt tcttcattta tatttttatc catcagttcc tgcattgcct tttgcagaag
     4861 tttacggtgg gaacgaattg ccaaacgcat tccaaaaccg tattcagcat tatcttcaaa
     4921 taagctgttt gcccaggcag gacctttacc atctttgttt ttacaatagg gaacagtagg
     4981 aaaagttcca ccccaaatgc tggaacaacc ggtagcattg gcaataatca ttctatcacc
     5041 aaagagttga gttaagagtt tcacataagg tgtttcacca caaccggcac aagcacctga
     5101 gaattccagc aggggttgtt taaactgact gcctttcacg gttgtttctt taaagttggc
     5161 gagaacatca ttgggcaatg tttcaaagta ttcgtaatta acttgttcgc ctttagttct
     5221 ttcttcttca atagggctca tcactaatgc ggatttagga cattcattca cgcagacctt
     5281 acagctttgg caatcgtcaa tataaacctg aactttatat tcataacccg gagcaccaat
     5341 tgcttttaag gtattaaaac tggctggctt attggcaagg tcttcttctt taatcagttt
     5401 tgcccggata gcggaatggg gacagacaaa agaacactga ttacattgaa tacaattttc
     5461 cggaagccaa tggggaactg aaggggctac ttttcttttt tccagtttag cagttccggt
     5521 ttcaacatag ccatctaaag gcatttggct aacttttacc tgatcacctt gttctcgcat
     5581 aatgggttca atcacattta agataaagtc atcggcatct tccggaacta atttcttcat
     5641 tggcatatat ttatcgggta gggtttcagg aacttcaatt tgcaccaatg cctcgttaac
     5701 tttgtccaca gcttccaaat tcatttgaac aaccttttcg cctttacgag cgtaggtctt
     5761 tctaatggaa tccttaatta aggcaatagc ttcattgcgt tccaaaacac cggaaataag
     5821 aaagaaagct gtttgcatcg tagtatttat tcttccgcca agaccaactt cttctgcaat
     5881 ctttaagcca tctatattgt aaaactttat ctttttgtta atgatggttt cctgcatcgg
     5941 acgggttaaa ttatagaatg cttcatctct actccagttg gaattcagca gaaaaacccc
     6001 attttctttt attccgccca gcaaatcaaa tctgccaata aatgcctgat tgtggcaggc
     6061 aacaaaatcc tcttgagcta ccagatattg gctgtgaatg ggggttttac caaaacgcaa
     6121 atggctacgg gttataccac cgcttttttt gctgtcatat tgaaaataac cctgaacata
     6181 catatcggta tggtctccga tgattttgat gctgtttttg tttgcaccaa cagtaccatc
     6241 ggaacctaaa ccccagaatt tgcaggaaat agtgccttca ggaacagtat tgattgtttc
     6301 ttcaagcggt aaagaaagat gagaaacatc atcctcaatg cctactgtaa aaccgtgaaa
     6361 gccattttta gccaagtgtt tataaacggc aagcaccatt gaaggagtaa attccttact
     6421 ggataaaccg tatctgccac cgattatgta tagttcagga cggtttttaa gagcagaaac
     6481 cacatccaga taaaggggtt caccaattga accgggttct ttggttctgt ctaaaacggc
     6541 aattcttttc acgctggcag ggattgccga taaaaagctt tgcacacaga aaggacggta
     6601 taaatgaact tttatcagtc ccaatttcat tccgcgcttg gcatttaacc attcaatcgt
     6661 ttcctgaatg gtttcacagc tacttcccat agctacaata atatcttctg catcgggaga
     6721 accgacatat tcaaaaagct gataatggcg tcctgttttt tctgctacct gctccattgt
     6781 ttctgcaatt atagcaggtg ccttggcata atataaatta ctggtttctc taccttgaaa
     6841 atatacatcg ggtgcttcct gaccaacctt cattcgcggt ttatcaggac ttaaagcatt
     6901 tttccgaaat gcttccacta aagaggggtc attcagttct gctattgttt cataatcaat
     6961 aacatcaatt ttctgcagtt catgactggt tcggaaacca tcaaaaaaca ccagaaaagg
     7021 aatccgagtt ttcattgtag caagttgagc cactaaaccc aaatccatca cttcttgcac
     7081 actattacag gcaattaagg caaaaccggt attacgagca ctcattacat cgctgtgatc
     7141 cccgaaaata gaaagggatt gagctgccaa agaccttgcc gaaacataaa aaaccgtagg
     7201 taacatctca ccggctattt tatgcatatt cggcagcatt aacatcaaac cctgagaagc
     7261 agtaaaagtt gtagttacag cacctgcgga aagtgaacca tgaacagcac ctgcagctcc
     7321 ggcttcactt tgcatttcaa taacatcaac cttggtgtta ttaatatttt ttctgccttc
     7381 ggaagcccag gcatcaacaa gttcacccat agtggaagaa ggggtaatag gataaatagc
     7441 tgctacttca gcaaaagcat aagcaatgtg agccgcagca gtattaccat ccattgtggc
     7501 tttggtctgt ttcgccattg tgtaactcct tatatttcat atttatatgt tttaataaca
     7561 gttacaagga caagatttga gaatgcgttt ttcgtcaatc taaatcataa agttagcgca
     7621 cagatgtcat agatgtctct cacagatttc acggatttta tggattttat ttacaaaaac
     7681 agcgctaaat gtatagccag aggatgctgt tcttccgtat tttttcagcc accgaaaacg
     7741 gcaagaacac aattagcatt aagaattatt tatgttatcc gctttatatt ctaactatat
     7801 tctttttcta tcatttatcc aattgttccc aagagcattg cttacccatt gctcacccat
     7861 tacggatggc attagcaatg agtaagcaat gggtaagcaa tgctattatg ggagaaaaaa
     7921 atttgtgatt gacaaaataa cattagttct gtaaaaggaa ataattataa attatatagg
     7981 tgaggtgaaa gtgagtgtag accaggaaaa aattaacgca atgagctttg aagaatcctt
     8041 aaaggaattg gaaaaaattg tagaccgctt atccacaggc gaaacaaatc ttgatgaatt
     8101 actatcgctg tatgaagcgg gagttgctta tttaaaacac tgtcaaagtc gtttaagttc
     8161 tgccgaggca aaaattaaaa tcctcagcga acagctttcc tcgcaacagg taacagaagg
     8221 caataatgga tagaaaaatt ttcctgaaga aggatatgaa agaaaagcag gagctggtaa
     8281 atatcatttt agaccgcttt ctcccgcgca aagatgaata tcctaaagac cttcataagg
     8341 caatgcgtta cagtgttttc gctggtggca aacgcttgag accctattta acaatgactg
     8401 cctttgaaat gtttaattcc aatattgaac ttattactcc cgttgcagca ggcattgaaa
     8461 tgattcatac ctattcgctg attcatgatg atttgccgga tattgacaat gacgacttca
     8521 ggcggggcaa aaaatcctgc catactattt ttggagaagg agttgctttg cttgccggag
     8581 atgctttact cctaagtgct tttgaactag tcactttggc tgaactggat gcaaaattgc
     8641 gtgtccaatt tgttcacgaa ttagctaaag aagttggtat caaaggttta attgcaggtc
     8701 agatggtaga tattgaatca gaaggcaaaa aagtggataa aaaaacccta cactatattc
     8761 acgaaaacaa aactgctcgc ctgataaatc taagtttgcg ttttggagct ctcgctggtg
     8821 aagctaaaga agaggaattg aaaattttgg atgaatacgg aaagttgatt ggactggttt
     8881 ttcagattgt ggatgatctt ctggatattg aaggaacgca agaagaactt ggaaaaacta
     8941 ttggtaagga tgctgaaagt gccaaagcta cttttcctgc tgtttacggt attgcggaat
     9001 ctcataaaaa agcagaagaa cttacagaaa aggcaagaga aattcttgct cccttagggg
     9061 aaaaagcatt aaagctggac attctggctg aatatctatt aggtaggaaa gaatgaatat
     9121 tctcgtgcaa aaactatctc cgactgctac tatcccgcaa aagatgacag aaaacgcttc
     9181 tggctttgat ctttttgccg atattggcga accaattatt ctaaatccct tggaatggag
     9241 acaaatttcc acaggcatag ctttagaaat tcccgtaggt tatgaagctc aaataagacc
     9301 tcgcagcggt ttatcttcca ctttgggact aagcatttta aacagtcccg gcactattga
     9361 ttccgattat cgcggtgaaa taaaagtgat tttgataaac cttagcccca atcctattcg
     9421 gattgaacct gaaatgcgaa ttgcacaaat tgtcttttgc cctgtaattt ctcctcagct
     9481 aattgaagct caaactcttt ctgaaagctt aaggcaagac gggggttttg gtcatactgg
     9541 acaataaaaa tgaaaaataa tgaactgatt caggaaataa aacacctgaa gaaagaaaaa
     9601 aatgcgctga ttttggctca taactaccaa attgtagaaa ttcaagacct tgctgattac
     9661 cggggtgatt ctttacaact ctctattttg gcgaaagaag ttaaagcacc tttgattgtt
     9721 ttctgcggag tgaaatttat ggcagaaact gctgccattt taaatcccga atctacaatt
     9781 ttacttcctg caattggtgc cggctgtcct atggcagata tgataagttt aagccagctg
     9841 aaagcattta aacaaaatta tcctggttct ccggttgtgt gctatgtaaa ttcttctgtg
     9901 gaagtgaaag ccgaaagtga tgtttgctgc acatcctcca atgctgttaa gattctgaag
     9961 tcctttagcg atgaaaagcc gattttattt gttcctgatc gcaatttagg ttcctgggca
    10021 ggtaaacaaa gcgggaaaaa agtaattacc tgggatggct actgtctggt tcatcaatat
    10081 ggtttcagtg aagaagatgt tttaactctc aaaaacgaat atccggatta taaactttta
    10141 gtgcatccgg aatgccctcc gcagattatt aaatatgccg atttggtaat ttcaacaggg
    10201 ggaatggtgg attgggtgaa aaataatgac aaagccataa tcgctacgga aattgggctg
    10261 acggaatatc tgcaacatat ctatccggaa aaatcgctta ttcctctttc ttccaaagca
    10321 atttgtaaaa atatgaaaaa gaccactctg gaaaatgttt ataacgctct aaaatataat
    10381 cagtatgtta taagcattga accaaacacg gcacaaaagg caagaaaagc tatagacagg
    10441 atgctggaac tttcaaggta agtaaaatgc tttttctatt caacctccat tctaaaaaaa
    10501 ggataacgct atgaaaaccc gctttttcct gattctttta accctgataa ttttattcgg
    10561 ggtttggctt tacctaagct acaatttggg cacaaacgaa aaagataaac tggaaaaatt
    10621 ggcaaatctg cctatctatg cttatgttgc agacacaaca aaggtagctc caattttaac
    10681 agagctgaaa acagttccgg gaattaaaaa tgtggtgcat gaaacagctt tgcaggcggc
    10741 tacggaactg attcagtcct acggtcttcc tttgaatgaa gagatgatta aggattacat
    10801 tttacctgat ataataacta taaatttgca gccaaaccgt gtttccataa gcaataagcc
    10861 cgttattatt gatatattac gctctcatat tccagaaaca gatatagaca gtcagtccag
    10921 tgcatatagt ttaataattc aagaacttag taatttagac ctatataaca ttgtgttcaa
    10981 tgtatttatt gccgtgctga ttttgctgat ttttgtgttt tcccgcactg ctttggaact
    11041 taacactctg ttgcattaca aagggattaa atatagtgct ttggaaaaaa tccgccatca
    11101 aagacaaggt ttgcaacata cattgttaat gctgattgtt cccttacctt tatgcttgct
    11161 tgcttatttt gcttatgtct attttaagcc cctacctcag gtaattccct attgggtgtt
    11221 tttagttcaa gcgggaactg taattttggg aactatgatt aactatttta ttttgcatag
    11281 ttttgaacag caagttgccc tacaggaaaa tcctattgaa gtgattactc ctccggaaat
    11341 ggaaagtaat ggaaacaaag aacaggaaaa tgacacgcca tttgcctaat attttaaccg
    11401 tcttcaggat aattctggta ccggtatttc tctatttcct gtttttagct aagctgccgg
    11461 caaatatttt gatagcactg attattttca ttgttgcctg ttttacggat tatctggatg
    11521 gaatgttagc ccgtaaaatg aacataataa gtgattttgg caagctgatg gaccctttgg
    11581 cagataaact gattgttctt tctgctttgg caggtttatg ttggcttcct ccttttcagg
    11641 ttagtattgt ggtctttttc ataatctttc tgcgtgaact tgtaattaca atcctgcggg
    11701 aaatttatca gaaaaaaggt attgtggtgc ctgcggattt tttcggcaag ctgaaaaccg
    11761 ttctgcaaat ggcaggaatc attgttgcct ttgctttttg ggtatttatt aatcccgttc
    11821 ctccgctggt aattctgata atcaatgtct ggttttggtt tgtggcattg attactgtgc
    11881 tcagcggttt aaactatatt attacgatag caaaaaggag taactatgcg taaatatatg
    11941 atttttctcc tgcttttact ggttctattc gcctgttcca aagaaggagg caaaatagac
    12001 cgttacacta tttacgataa agtaattgac cattccaaac ccttagctat gggagatgac
    12061 agggatgtgt atgtattttg tgattctctt gattggcaag gtcttaaacc ttttattggc
    12121 agtgctatag aacaacagat aattatagtt tatccagagc agtattttaa cctaatgatg
    12181 gctgatatca aggatgtgaa aagattatcc aaatataaaa atctcgtttt tattggtcac
    12241 ctggaaagcg atttacccgt ttctgcctat atgaaaggag tgctcagtca ggattttatt
    12301 acaagagtgc aacaaacagg tggcgaactt tttaccagtg aaaacctcaa ttgctgtgat
    12361 cagattgtgc ttttcttgtt aggtaaagat gcgaaatccc tgcaaaaaat aggtgcccta
    12421 caagcagaaa atatcttttc gctgctctta aaacgcttta cgaaccgcca gggttattat
    12481 gcttatcagg gaaaagtgat agacccctcc ttttttgata attatccttt ttccctgaaa
    12541 attcctgata cctatcgttt gtattccaat gataaagttg gcaggtttct ttgttttctt
    12601 tatagagccc gactggaaaa tagagaaatt cccgataaat acatttctgt ttactatgaa
    12661 ccgatgccgg aaaacaaagt ggacttaaat tggctgattg caaaacgcca ggaaatagga
    12721 aaaaaatact ttgaaggaga tgaatttgat cctgaacttg tgcggaaaga accctttact
    12781 tttaaaaaac atcctggcta tcgtctcttg ggtgcctgga aaaatatgaa atatgcaatc
    12841 ggaggcggct ttcaaagtta tggcttttgg gatgaaaaaa ctaaaactgc ttacattgta
    12901 gatagcagcg tttattttcc tgctggagac aaacttcccg ttctggtgga attgtatgtt
    12961 atctccaatt ccctggaaat taaatgaaaa ctatcttgtc ttgcaagcag ttactactgt
    13021 ggctggcttt tttccttttg cttttatctg cttgtgcagg acctcaaaca acagctcaaa
    13081 gaactattat agataattct caacccaata ttccttctct ttattattat atttctgctt
    13141 cgttaaaaca ttatgaaggg gactttctaa cagcagataa ttattatcat ctggctttgg
    13201 aacaggactt tcgtagccat cagattaaaa aacagatttt aattaatagt gcttatgcct
    13261 ttttaagtaa acaacaggat gcagaaataa cggttaggca atttgaccaa gccagaaaag
    13321 aaatgatttt tgataatgat cttttaaatg ccgcttattc agtttatagc aatgccaata
    13381 atgaagaagg acttgcctgg gtagtttcgg aaagtgtgtt atattatcct tccactttaa
    13441 gttttctgcg taaattctat ttggattaca gcaaaaacaa aaagagtgat gaaaaccttc
    13501 tggaacttgc tcttaaatat gcagataaca atcctgaaga tataattctt accgctaaaa
    13561 tgtataccct ggtaaatcct aaacgctccg tatctctttt acaagaggca tacgaactgg
    13621 agcccaaaaa agaaacagat aatctgctta gtgaaattac actccaatat ctgggaaaag
    13681 aagaagctct gaaaaaattc aggacttaca gttatcctga agataaagac aggatgttat
    13741 actttttgca gatggcaaat cgtaatcgtc gcctggaagt agttaatact ttaagcccta
    13801 tattgctgaa tacaggtgat tcttctttga taggagaact ggctttttct gcatatctgg
    13861 aagataaaac agatatcttg caggaaattt cccgcttttt attaaccaaa gatgcagacc
    13921 ctcttaccga ttctaaagtg gtttcctttc tttttgccga agctcttttt tccgaaaagt
    13981 tgccggaacc ttccctttat cttgattact ttaattcggc acaagatgtc caagatgtga
    14041 ttttgtatgc aatgctgaaa cgctctatgc aggcacaatc ccttgaaaaa gaaacaaata
    14101 tcatattttc ggaagaattt gtttctgcag ttgagaagcg tctgccggaa aatgtttttt
    14161 cccgttatct gaaaattgct tcacaaatta agtccgttga agatgaggac tttcttcaag
    14221 cgagggctga actttgcagt tacttcctgc aaaaagactt aggggaactg gaggattgga
    14281 ctttcctgct tcagtattac caaaatgagg gcagggaaga agaaaaaatt gctactctgc
    14341 gtaaagctgt tcataagttt ccggataatc ccttatttct taatgacttg ggttattccc
    14401 ttttggacta tcctcagttt attgtagaag gtggctttct tattcaaaga gctgtcgctt
    14461 tagaccctac aaatccttat tatcaagata gcttagcttg gttctattat ctaactgcag
    14521 atttttctaa ggcaatggaa catatcacaa ttcctatgca aatgaaaaat gctccgggag
    14581 aaatatccta tcatattgga atgattcttt tggctaatca acaaaaagaa gaggcaatta
    14641 aatacttcca atccgccttg gaagatgaac aaacccccat ctatcaggaa aaagcgaaaa
    14701 aagcactaaa cgaactaaaa accgactaaa caatagagca gtgggcgaag aaaaatattt
    14761 gatttatatc cattgaaaag ttttgtagga atgatagata aagttgaagg atctgaaccc
    14821 tgcgcaagaa agatgaacga tagcttattt cttttttaca aacgagttcc gtaggaacga
    14881 tagataataa caacgggttt ttaacccggt gcaaggggaa aaaaccactc aactacactt
    14941 ctatgaagaa aaaagttccg taggaacgac agatattaac aacgggtttt aacccggtga
    15001 taagcaagca gaaaagtcat tatctccttt cccgagaaaa agttccgtag gaacgacaga
    15061 tactaacaac gggttttaac ccggtgatca ggatagcttt agatagctta tttctttttt
    15121 tgagaatgag ttccagagga acgacagaat tatgatttct ctgataaatt ttcatttttt
    15181 ctggttaatg acttccttct ctacataagt cctatctatt ttattgtata tttacctgct
    15241 ggatcttttg ctttttcttc ttgttgcttt ttgcgggttg aaacccgcta ttagcatctg
    15301 tcgttcctac ggaacttttt attatagtgg taaggttttt ctcattttca ccgggttcaa
    15361 aacccgttgt tagtatctgc catccctaac gggatttttt tgatcttaac aatgggtttc
    15421 aacctgtgga aaagaaagca gaaaagtcat tacctccttt cccgagaaag agttccgtag
    15481 gaacgacaga tactaacaac gggttttaac ccggtgataa gaaagcagaa gagccattac
    15541 ctcttttttc cccagaaaaa gttccgttag gaacgacaga taatagcgtc gggttttgaa
    15601 cccggttc
//
LOCUS       DAOPFL010000036        13801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5177_length_13801_cov_94.911611, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000036 DAOPFL010000000
VERSION     DAOPFL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13801
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5177_length_13801_cov_94.911611"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..2047)
                     /locus_tag="PLG69_04085"
     CDS             complement(185..2047)
                     /locus_tag="PLG69_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPI42723.1"
                     /translation="MNIPLNIYVAYFEKGILNFGYVIAQNNNRLEVITETGNYVVLPE
                     SRIILQSKEKYLESEPYQALPNFINQVNLFEEQFQESDFHFLKEKECTLQEIATKLNL
                     QTDVQIFALFKYLHNHPKEIHCKKNKYRLKTPEEITQYQLQLQQQEEERQFLQDVNAF
                     FSGAELSRESQHKLYNALPELQTAKKYKKLKELILSQYPLLKPEEAILEFRKFCGETP
                     EYIDPVIANAGIPIGFSSLLIEEKLLPWKVTQPEAIAFSIDDESTKDFDDAISFTKDG
                     SFWHLTLYVSSVSERLNLEGALFAEAKKRVSSLYTANAVIPLFPFNHSEQELSLKKDS
                     VRPVLALNIWLDENLAIQHYELSRMNITISENYSFNEIDHQIEQEPFSTLYRLSKLLA
                     EKRGANSFSEKERYYYYISAAEQGLEVKCVDTQSPARKIVEELMILYNSYLADYAVKN
                     NIPVIYRNINQFEDPKDNYLSLQAYLSTEPEFHPGIGANAYLHSTSPIRRYVDLINQL
                     QISAILQGKNPPFSKEELEKDIPLLEKRIYDIREIAHKSERYWVLKFLQQNCINIPLE
                     TYFRISIDGYLSFELTPWGFIITAKSESYPKTERCKIVIYKVDPEQGIVWVDII"
     gene            complement(2072..2147)
                     /locus_tag="PLG69_04090"
     tRNA            complement(2072..2147)
                     /locus_tag="PLG69_04090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2112..2114),aa:Arg,seq:cct)
     gene            2235..3263
                     /locus_tag="PLG69_04095"
     CDS             2235..3263
                     /locus_tag="PLG69_04095"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPI42724.1"
                     /translation="MAAIMLCQIDKGKQIKMKVAIVGATGEVGRMMITCLEESNLEIS
                     ELDLFASSRSAGTVLYYTDQPIKVQELKQDSLLKHYDYVLFSAGAGVAKTFAPIAIQT
                     SEVVIDNSSGFRKEPNIPLVVPEVNGYLLTGYSGIVANPNCSTIQIILPLAVLDKLFN
                     LKKMIVSTYQSVSGSGHQGIVTLLNQRKGKKDKGIYPHIIDLNVIPQIGGFLDSGYCQ
                     EEEKMHFECRKILNKWELLVSATTVRVPVIYGHSVSVYAEFENEVDIPKVEKTLQNSP
                     RIVYYPNTYITPLDLGSSNETHICRLRLGTDEKSLTFWNVGHNVRLGAAANAVNILIT
                     HSKYSGRL"
     gene            3263..4384
                     /locus_tag="PLG69_04100"
     CDS             3263..4384
                     /locus_tag="PLG69_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPI42725.1"
                     /translation="MIDIIELRHSLHRIPELAFHEFKTKAMLEENILRLLQNQPENLW
                     HLHYFQNSPGLLLEYSPAKGNYLLFRADMDGLPIAEKTGVPFASEHPNLMHSCGHDVH
                     LSILMGLIQYVANTLPQRNILFLFQPAEEGEGGAKSILAEGIIQKFEIASVFALHIAS
                     ELPVGSVSTKEGIFFAVSQEFDVLFYGKASHIAFPEKGIDALSAGIKFMNNIEDKLAE
                     LKKTEKIIFHIGKMSSGTIRNVIADKCILEGTHRSLSPKISQKINILIKDTAELIARD
                     TNTRSEVKFLASYEPVINNPELVKRLQKICRQQNIEYIPAQTSMTGEDFGFFTTLYPG
                     LLFWLGSGCSYPLHSDKFLPRDECLEIGVKIMSAFLTTE"
     gene            complement(5119..10572)
                     /locus_tag="PLG69_04105"
     CDS             complement(5119..10572)
                     /locus_tag="PLG69_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42726.1"
                     /translation="MKRSIALILILLAGCIVFANINDNLPANLNSAFTLGAKNSSSLQ
                     VNFTLPEYSMQEETYGGAVYHKIILPLSGTLMETGMPELPVVCTSIAIPHTGGVNIEV
                     LSTQQTVIPNFLPYPVQQGNSSESPKGFIINNEYYNSGNNYPEMLIEYSEPSILRDFR
                     IITIQINPFSYNAQTGELTVNHNIEFRLNFTQEQGINELPNEPANISASFDKIYDSMI
                     LNYGDYRDAVVTNTPPRYLIIYGTNSDTNFTSALNEYVLWKKQKGADVMVASTAANEA
                     GNTTTSIKNYIQTKYNNPETRPDFVVLIGDTSGSYPIPAYSAANSGVTDYNFTFLAGN
                     DQLGDCFIGRISVENLSQFLVVLNKIYLYERDINLATASWLNKMMLSGDNAPSGISTM
                     YIHKYIKEMSLLTNPDYTFTEDYGASPNYTIINQALNQGVGFYSFRGYIDWSPPAESA
                     LFNGYKLFHTVNITCGTNNYSGAGEVEGMIRYGTTAAPKGAVTGIGMCTSSTHTTFNN
                     AIHGGIFAGIFASDMRTMGEALLNGRLYLHQLFGVSSPTNATNFAAWCNLMGDPTMEV
                     FTGIPNHFDVTSDISIPLGLKLYDVAVQDNSGNPVEGAAVVLNMGGLILARNYTGADG
                     TAILVLPSDMLAGNATLTVSKHNFKPIQTTISVVNMATLVPGTMVIDDDNIAPSQGNG
                     NAIASSGETIELFLGLTNTGTDIITGINGSVISNSPYITITNSSVIYPDILGGETGNN
                     SVPIIIQIAPDTPHQTMLRLYLNLTDSNSVSYHISEFIPVEAPNIEFVSYLVMDSNNQ
                     HLDPGEVAEFSITVKNTGTVEADNVLGILYTQNDLVGVSDPNAEFGSLPTGILVTCGT
                     NRFILSARTEVLPGMLIPMRLKLYNSDGFEQFVDFTFTVGVVTQQDPLGPDTYGYVIY
                     DWSDTNYAEAAVYDWIEISPLEGGLGTTLAISDGYASGNEGDQVGSDALEVVSLPFPF
                     QFYGILYNQITVCSNGFIAMGVTANAEFRNYRLPGPMGPNPMIAPFWDDLATGTGSGI
                     YTWFDRNHHSFIIEWYKLKNGNGGTALETFQCILYDQAAYPTSFGDGPIKFQYQTFNN
                     IDSQSGNKHGNYCTIGIEDHTGKVGLEYTFNNTYPTAAAPLSNGKALFITNVPIYHSS
                     AHLLLEATYVNDTNMNGICEPAEIVELGAKIQNTGNLTAENVIGQISTASEYVTINNA
                     TAEYYPIEPGMNGVNKEPFTFTIANNCPDGEVLYFELLITSGELQWTRNFSLQVSASC
                     LLYHSFFVDDHLNDFDGMVSSGEFFNLIVNLENFAAVDATSIQATLSSPLQQLIIEEP
                     LLQFDKIGPNDIYQIKFSINTSMLDVSVTQIPLFFTATASNGSSTNASFSIPYNNPVV
                     FQDFELNNGYFVSETGWVWGNPSQVTPFSGAKVWATNLSGNYPSYVNYNLYTPKYLLE
                     TNSQLQFRHYYSCEYGNDGGNVSISTNNGVVWNLIYPVSGYNGYSLNGLNGDPGWTGN
                     SDGWQLAIFDLSQYAGQSVMFRFRFGSDGEISGPGWFIDDFTVSGVNLKTGYLYGVVY
                     PSSGLDPSMATLRSNQRFTSHPDSEGNFIIFLPNGIHTVTATMPYHQSSTLTSIIINP
                     SNPVHYTEFTLIDLPQPQSISYIGNNDTGDLYVFWQPPEDTVLPIVGFKVYRKFNTDQ
                     FYLVQDSPEISYHEVLTLPGHYRYYIVVKYLNTDGTPSQILNIPFPFIPNSDYEIPAL
                     VTKLNNNYPNPFNPTTTISFTLAKSGKTTLTIYNLKGQQIKRLVNEELPSGMHNVVWN
                     GKDEKGRNVASGVYFYRLQSGNYQATQKMLLMK"
     gene            complement(10638..12059)
                     /gene="glnA"
                     /locus_tag="PLG69_04110"
     CDS             complement(10638..12059)
                     /gene="glnA"
                     /locus_tag="PLG69_04110"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPI42727.1"
                     /translation="MELKELQHLILANEVKAVDLKYCGLDGKWYHITFPARNIAEVME
                     VGIPFDGSSIPGMRSVESGDMVLMPDSKTAHLESFYETPTLRMICSICDAETRLGVKK
                     DPRSVALRAHNYLLSTGIADMSTWIPELEFHLFDSVQYCSDSFSAGYNITSSECKEAL
                     PDDNEDPGSLSLQGVKGYHIDTPFDQFYEIRQKIVELIEEQDIKVRYHHHEVGISAQE
                     EIETELLAFPKICDDVMIMKDIIRKTALQYGLTATFMPKPVFGHAGNGMHFHIMLHKN
                     GKNLFYKKGGYADLSEEAIWFIGGILIHGRALVAFTNPSTNSFKRLLPGFEAPVKLFY
                     GLANRSAAIRIPKYANTPETKRFEFRTGDGTCNPYLAMSAILLAGLDGIKKQIDPAKY
                     NLGPFDDNVFAWSEDKKAQLLSIPTDLKEAMQALKEDHQFLLEGDVFNEELIESHIEL
                     KLKENEAVSARPHPHEMLLYYNL"
     gene            complement(12454..13101)
                     /locus_tag="PLG69_04115"
     CDS             complement(12454..13101)
                     /locus_tag="PLG69_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPI42728.1"
                     /translation="MISPNIIKLLVNAVIIFLVFYSIIIHEFSHSLMAYWLGDDSAKR
                     AGRLTLNPLKHIDWFGTVILPLFLYLTCGFIYGYAKPVPFNPYNFKNLKRDSGLTGLA
                     GPLSNFLVAIFFALIFHLCGFSVVIKYICFFVIYLNLLLAFFNLIPVPPLDGSKVIGM
                     FLTDQAYYKWTMQERKGMMYLFAFIIITTLLGWNIIGKIIIPPVNFVMQIMGVRI"
     gene            complement(13098..>13801)
                     /gene="tyrS"
                     /locus_tag="PLG69_04120"
     CDS             complement(13098..>13801)
                     /gene="tyrS"
                     /locus_tag="PLG69_04120"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPI42729.1"
                     /translation="HEILYPVLQGYDSVAVNSDVELGATEQKFNILCGRDMQRHFGME
                     EQVAILSPILIGTDGVNKMGKSLNNYIAVFDPPTEKYGKVMSIPDNIILNYFYYATTM
                     PEEEIEKIKKELESGTNPMLIKKRLAREIVTLYHSAEEAKEAEYAFEKQFSLREVPND
                     IPEFVVSKNSCKLSNLLVDSGICSTNGEAKRLIQGGAVSLNGEKITAFDAVVNLKDGD
                     IIRAGKRNYIKIKKI"
BASE COUNT         4424 a         2654 c         2387 g         4336 t
ORIGIN      
        1 ctaattatta cacttacaac cgaactttca caggtttaga attcaaaaat tgtgagctaa
       61 aacaaaacaa agaactgaga aacaaagcgt tggaatttac accaaccttt gagacactac
      121 ccttcctatt tttttatctg cgaaatctgt gttatctgtg agagttcttt tatcagcgtg
      181 aggtttaaat tatatccacc cacacaatac cttgttccgg atccactttg taaataacaa
      241 tcttacatct ttcggttttc ggatagcttt cgcttttagc agttataata aatccccagg
      301 gtgtgagttc aaagctgaga tagccatcaa tacttattct aaaatatgtt tccagaggaa
      361 tatttataca gttctgttgc aaaaatttca gcacccaata gcgttcgctc ttatgagcaa
      421 tttctcttat atcatatatt cgtttttcca aaagaggaat atctttttcc agttcttctt
      481 tgctaaaagg aggatttttc ccttgcaaga ttgcggaaat ttgtaattga ttaattaaat
      541 ctacatagcg tcggataggt gaagtagaat gtaaataagc atttgctcca attcccggat
      601 gaaattccgg ttccgtggaa agatatgctt gaagggataa ataattatcc ttggggtctt
      661 caaattgatt gatattgcga taaataacag gtatattatt ttttacagca taatcagcca
      721 ggtaagaatt ataaagaatc atcaattcct caacaatttt tctagctgga ctttgagtat
      781 ccacacattt cacttccaaa ccctgttccg cagcagaaat gtaataataa taacgctctt
      841 tttccgaaaa agaattggct cctctttttt ctgccagtaa cttactaagc cgatataaag
      901 tagaaaaggg ttcctgctct atttgatgat caatttcgtt aaaactgtaa ttctcagaaa
      961 tggtaatatt cattctgctt agttcataat gctgaattgc cagattttca tccaaccata
     1021 tatttaaggc aagaaccgga cgcacactat ctttttttaa actaagttcc tgttcggaat
     1081 gattaaaagg aaataaggga attaccgcat ttgctgtata aagagaacta accctttttt
     1141 tagcttcagc aaataaggca ccttctaaat tcaatcgttc cgaaacggaa ctaacataaa
     1201 gagttagatg ccaaaaagag ccatctttgg taaaacttat agcatcatca aaatcctttg
     1261 tgctttcatc atcaatggaa aaagcaattg cttcaggttg tgttactttc caaggcagaa
     1321 gtttttcctc aatcaataaa gaagaaaaac caataggaat tccagcattg gcaataacag
     1381 gatcaatata ttcaggtgtt tcaccgcaaa atttacgaaa ttcaagaatt gcctcttcgg
     1441 gtttaagcaa tggatattgg gaaagaataa gctcttttaa ttttttatat tttttagccg
     1501 tctgcaattc gggcaaagca ttataaagct tatgctgact ttctctgctt aattctgcac
     1561 cggaaaaaaa ggcattaaca tcctgtaaaa attgcctttc ttcttcttgt tgttgaagtt
     1621 ggagttgata ttgagtaatt tcctccggtg ttttcagacg atatttattt tttttacagt
     1681 gtatctcttt ggggtgatta tgtaaatatt taaaaagtgc aaaaatctgc acatctgttt
     1741 gcaaatttag tttagttgca atttcctgaa gagtgcattc tttctctttt agaaagtgga
     1801 aatcggattc ctgaaattgc tcctcaaata aattaacctg gtttataaaa tttggcaatg
     1861 cctgatacgg ttcactctcc aagtattttt ctttgctttg cagaataata cgggattcgg
     1921 gtaaaactac ataatttcct gtttcagtaa taacttcaag acgattatta ttttgagcaa
     1981 ttacatagcc aaaattaaga atccccttct caaaataggc aacataaata ttcaagggta
     2041 tattcaccaa accttctcaa ttatttattt ttggcgcccc agagatgact tgaacatccg
     2101 accaacggtt taggaaaccg ctgctctatc cactgagcta ctggggcgca tttttattta
     2161 ccaatctgca aagagagctt ttttgtcaag tttacattga caagctatgc ttgctggaaa
     2221 agtgtgaaaa ataattggca gctattatgt tatgccaaat tgataaagga aaacagataa
     2281 aaatgaaagt agctattgtt ggtgccacag gtgaagttgg caggatgatg atcacttgcc
     2341 tggaagaaag taatttggaa atttccgaat tggatttatt tgcttcctca aggtctgcag
     2401 gcacagttct ctattataca gatcaaccaa ttaaagttca ggaattgaaa caggattctc
     2461 ttttaaaaca ttacgattat gttttgtttt ctgccggagc cggagtagcc aaaactttcg
     2521 ctccaattgc tattcaaaca tctgaagtag taattgataa ctcttccgga tttagaaaag
     2581 aacccaatat tccacttgta gttccggaag tgaatggtta cttgttaacg ggttacagtg
     2641 gcatagttgc caatcctaat tgttctacta ttcagattat tttgccttta gcagtattgg
     2701 ataaactctt caacctcaaa aaaatgattg tttccaccta tcaatccgtt tccggaagcg
     2761 gtcatcaggg aattgtaact ttattaaatc aacgcaaggg gaaaaaagat aaaggtattt
     2821 atccgcatat aattgatttg aatgttattc ctcagattgg aggttttttg gatagcggat
     2881 attgtcagga agaagaaaaa atgcactttg aatgtcgtaa gatacttaat aagtgggaat
     2941 tacttgttag tgccacaact gttcgcgttc ctgttattta tggtcacagc gtttctgttt
     3001 atgcagaatt tgaaaatgag gtagatatac ctaaggtaga aaaaacatta caaaattcac
     3061 ctcgtattgt ctactatccc aatacttata taactccgct tgatttaggt tcttccaatg
     3121 agacccatat ctgcagattg cgtttgggaa ctgatgaaaa gtccctaacc ttttggaatg
     3181 taggacataa tgtacgttta ggagctgcag ctaatgcagt taatatttta attacccatt
     3241 ccaaatattc aggtcggctc taatgattga tattattgaa ctgcgtcata gtttacatcg
     3301 tattccggaa cttgcttttc atgaatttaa gaccaaagct atgctggaag aaaacatttt
     3361 gcgtctttta caaaaccagc cggaaaattt gtggcatctt cattattttc aaaacagtcc
     3421 cggtttgcta ttggaatatt ctcctgcaaa aggaaactat ctgttattta gagctgatat
     3481 ggatggctta cctatagcag aaaaaacagg agttcctttt gcttcggaac atcctaattt
     3541 gatgcacagc tgtggacatg atgtacatct aagtatttta atggggttga ttcaatatgt
     3601 agcaaatacc ttaccccaac gaaacatttt gtttcttttt cagccagccg aagaaggtga
     3661 aggaggagca aaaagtattc ttgctgaagg cattattcaa aagtttgaaa ttgcttctgt
     3721 ttttgctttg catattgcca gtgaacttcc tgtaggttct gtttctacta aagaaggtat
     3781 attttttgct gtttcccagg aatttgatgt tcttttttat ggcaaagcca gccatattgc
     3841 ctttccggaa aaaggaattg acgctctctc tgcaggaatt aagtttatga ataatattga
     3901 ggataaattg gcagaactga aaaaaacgga aaaaatcatc ttccacatcg gaaaaatgag
     3961 ttccggaact attcgtaatg ttattgctga taaatgtatt ttggaaggaa ctcaccgttc
     4021 tctctcccca aaaatcagcc aaaaaataaa tatccttatt aaagatacag ctgagctaat
     4081 tgccagggat accaataccc gaagtgaagt taaatttctt gcttcctacg aacccgtaat
     4141 caataatcct gaattagtaa aacgcctgca gaaaatctgc cgtcaacaaa atattgaata
     4201 catacctgct cagacctcta tgacaggtga ggactttggt ttttttacta ctttatatcc
     4261 aggattgctt ttttggttgg gtagcggatg ttcctatcct ttgcattcgg ataaattttt
     4321 accccgggat gaatgcctgg aaatcggtgt aaaaataatg tccgctttcc tgacaacaga
     4381 atagtcccgg atttttttac aaagagaaaa agaggataaa tggataattg tgaatgcaaa
     4441 atgtagaatg aaaaattgaa tttaacctaa aaaatgcagc tgcaggaaaa tgggttgtca
     4501 aattctgtct ttgctaaaaa attcgtagaa ctacgaaaaa taactgtcct caatttatag
     4561 ctaatctgaa ttcagcaaac caaatgctaa atttatcttt tcttcccagg gttaaaaccc
     4621 ttctctaata tctgtcgttc ctacggaact ttttctcgga aaaagagata atgacttttt
     4681 gctttctttt caccgggtta aaacccgttg ttagtacctg tcgttcctac ggaacttttt
     4741 tctttcatag ctacattttt gcaccgggtt aaaacccgtc cttatctaac tatcattcct
     4801 acggaactaa tattcggaat cttgtaaatc ccttttatcc tgttcatcct atacctattc
     4861 tcatttttaa tctgcgtcat ctgcgaaatc tgcgtgagcc ctttttttca ttctcctctt
     4921 actcgccggg ttaaaacccg ttgttaatac tgtcattcct acggaactga tactccaaat
     4981 tatataaatc ccttttatcc ttttcatccc ctacctattt tctctctgcc ctctgcacaa
     5041 atcatcctgt tcatcccata cctatttttt ctctgcccta agctctctgc cttttaaacc
     5101 ctctcaacct tctaaacttt atttcatcag cagcattttt tgggttgctt gatagtttcc
     5161 actttgcaga cggtagaaat atactccgga ggcaacatta cgtccctttt catcttttcc
     5221 attccagacc acattatgca ttcctgaagg aagttcttca ttcactaagc gttttatctg
     5281 ctgtccttta aggttataaa ttgttaaagt tgtttttccc gatttagcca aagtaaaaga
     5341 tattgttgta gtaggattaa aaggattggg gtagttatta ttcaatttag ttactaaggc
     5401 aggaatttcg taatcagagt tcggtataaa aggaaaggga atattaagta tctggcttgg
     5461 agttccatca gtgtttaaat atttaactac aatataatat ctataatgtc ctggtagagt
     5521 gagcacttcg tgataggata tctctggaga atcctgaacc aaataaaact ggtctgtatt
     5581 gaatttccga tataccttaa aaccaacaat aggcaaaaca gtatcttccg gtggctgcca
     5641 aaagacatac aaatccccgg tatcattatt accaatataa ctaatacttt gtggttgagg
     5701 caaatcaatt aaggtaaatt cagtataatg aacaggatta ctcggattga taataatgga
     5761 agttaaggtt gatgattgat gatatggcat agtagcggtt acagtatgaa tcccgttagg
     5821 caggaaaata ataaaattac cctcggaatc aggatgactg gtaaaacgct gattagacct
     5881 aagagttgcc attgaaggat caagacccga acttggataa acaactccat acaaatatcc
     5941 ggttttcaaa tttaccccac taacggtaaa atcatcaata aaccatcccg gaccggatat
     6001 ttctccatcc gagccaaatc ggaaacgaaa cataaccgac tgtcctgcat attgcgatag
     6061 atcaaaaatg gctaattgcc acccatctga atttcctgtc cagcccggat ctccattcaa
     6121 accgtttagg gaatatccat tgtaaccgct aacaggataa attaaattcc aaacaactcc
     6181 attatttgta gaaatagaaa cattaccgcc atcatttcca tattcacagc tataataatg
     6241 cctaaattga agctgactgt ttgtttccaa taaatacttg ggagtataga gattataatt
     6301 tacataagaa ggatagtttc cacttaagtt agttgcccaa accttggctc cggagaaagg
     6361 tgttacctga gaaggatttc cccaaaccca accggtttcg gaaacgaaat aaccattatt
     6421 taattcaaaa tcttgaaaga caacaggatt attgtaaggg atactaaatg atgcatttgt
     6481 tgaactacca ttggaagcag tagcagtaaa aaataaggga atttgagtaa cagaaacatc
     6541 aagcatagag gtattgatcg agaattttat ttgataaata tcgttaggac caattttatc
     6601 aaattggaga agcggttctt caataatcag ctgttgcaat ggactgctta aagttgcttg
     6661 aatactggtc gcgtcaacag cggcgaaatt ttccaaatta acaatcagat taaagaattc
     6721 accactgcta accataccgt caaaatcatt tagatgatca tctacaaaaa atgaatgata
     6781 gagcaaacaa gatgcagata cctgcaaact gaaattcctt gtccactgca attccccgga
     6841 agtaatcaac agttcaaaat acagcacttc cccatcagga cagttattcg caatcgtaaa
     6901 agtaaagggt tctttgttca caccgttcat tccaggttca atcggataat attcagcagt
     6961 ggcattattg attgttacat attcacttgc agtgctaatc tgtccaatta cattttccgc
     7021 tgtcagattg cctgtattct gaattttagc ccctaattcc actatttcag ctggttcgca
     7081 tattccattc atatttgtat catttacata agttgcttcc agaagcagat gagccgaaga
     7141 atgatatatg ggaacattag taataaataa tgccttaccg ttacttaagg gggctgcagc
     7201 tgtaggatag gtattattga aagtatattc caagcccact tttcccgtat gatcttcaat
     7261 accgatagtg caatagttgc catgtttgtt tccggattga gaatcaatat tattaaatgt
     7321 ctgatactga aatttaatag gtccgtctcc aaaacttgta ggataagctg cctggtcata
     7381 taaaatacac tggaaagttt ctaaagcggt tcccccattg ccatttttca gtttatacca
     7441 ttctattata aaggaatgat gatttcggtc aaaccaggta taaattccac tccctgttcc
     7501 agttgccaaa tcatcccaga agggagcaat cataggattg ggacccatag gtccgggaag
     7561 acggtaattt ctaaattccg cattggcagt aacccccatc gcaataaaac cattggaaca
     7621 aacggttatt tgattgtaga ggatgccgta aaactgaaaa gggaaaggta aggacacaac
     7681 ttccaaggca tccgaaccaa cctggtctcc ttcattgcca cttgcataac cgtctgaaat
     7741 agctaaggta gttcccaaac caccttccaa aggcgaaatc tcaatccagt cataaactgc
     7801 ggcttcggca taattggtat ctgaccaatc ataaattaca taaccgtaag tatcaggacc
     7861 aagaggatcc tgttgagtta ccacacccac ggtaaaagta aaatccacaa attgctcaaa
     7921 accatcacta ttataaagct ttaatcgcat aggaattagc attccgggca aaacttcagt
     7981 tctagcagaa aggataaaac gattagttcc acaggttacc agaattcctg ttggcaaaga
     8041 gccgaattca gcatttggat cacttacgcc aactaagtcg ttctgagtat ataaaatgcc
     8101 caaaacattg tctgcttcaa cagttcctgt atttttaacc gtaatactga attctgccac
     8161 ttctcccgga tcaagatgct gattattact atccattacc aaataggaaa caaattcaat
     8221 attaggtgct tccacaggaa taaattctga aatatggtag gaaacggaat tagaatccgt
     8281 aagattaaga tataacctta acattgtttg gtgaggagta tctggagcaa tttgaattat
     8341 gataggaact gaattatttc ctgtttctcc acctaaaatg tcaggataga taactgaaga
     8401 attcgttata gtaatatatg ggctgtttga aatcacactg ccgttaattc cggtaatgat
     8461 atctgttccc gtattggtta agcccaaaaa aagttcaatt gtttctccgc tactagcaat
     8521 tgcatttcca tttccttgcg aaggtgctat attatcatca tcaatcacca tcgttccagg
     8581 aaccagagtt gccatattta caacagaaat tgttgtctga ataggtttaa aattgtgttt
     8641 gcttaccgtt aaagttgcat ttccggcaag catatcggaa ggaagcacta aaatagctgt
     8701 tccatctgct cctgtgtaat tacgagctaa aataagccct cccatattta aaactacagc
     8761 agcaccttct acaggatttc ccgaattatc ctgaacagca acatcatata attttaatcc
     8821 caaaggaatt gaaatatcag aagtaacatc aaagtgattg gggatacctg taaagacctc
     8881 catagtagga tcacccatca aattacacca agcagcaaaa ttagttgcat ttgttggaga
     8941 agaaacccca aagagttgat gtagatagag gcgtccattt aataatgctt cacccatagt
     9001 tcgcatatca gaagcaaata ttccggcaaa aattcctcca tggatagcgt tattaaaagt
     9061 agtatgggtg ctggaagtac acatccctat tccggttacg gcacctttag gagctgctgt
     9121 tgttccatag cggatcattc cttccacttc tcctgcacca ctgtaattat tagttccaca
     9181 ggttatattt acggtatgaa acagtttgta gccattaaaa agagctgatt ctgcaggagg
     9241 agaccaatca atataacccc tgaaactgta aaaaccaact ccctggttaa gtgcctgatt
     9301 aataattgtg tagttgggag aagcaccata gtcctcagtg aaagtataat cgggattggt
     9361 aagtaaactc atttctttga tatatttatg aatatacatt gtggaaattc cggaaggagc
     9421 attatctccc gaaagcatca ttttattcag ccagctggca gtagctaaat taatatcgcg
     9481 ttcataaaga taaattttgt tcagaactac caaaaattgc gataagttct caacagaaat
     9541 acgcccgata aaacaatcgc ccagttgatc gtttcccgct aaaaaagtaa aattataatc
     9601 agttactccg ctattagcag cagaataagc agggatagga taacttcctg atgtatcacc
     9661 aattaaaaca acaaaatcgg gacgagtttc gggattatta tatttagttt gaatgtaatt
     9721 tttaattgag gtagttgtat ttcccgcttc attagcagcc gtgctggcaa ccattacatc
     9781 tgcgcctttc tgttttttcc agagtacata ttcattcaaa gcactggtaa aattagtatc
     9841 actgttggtg ccataaatta ttaaataacg gggaggagta ttggttacta cggcatcacg
     9901 ataatcacca taattcaaga tcattgaatc atatatttta tcaaagctgg cagaaatatt
     9961 agcaggttca tttggcaatt catttatacc ctgttcctga gtaaaattta ggcggaattc
    10021 aatattatga tttacagtca gttcacccgt ttgagcattg taagaaaagg gatttatctg
    10081 gatagtaata atccgaaagt cccttaaaat acttggttca ctatattcaa tcagcatttc
    10141 cggatagtta ttaccgctat tgtaatattc attgttgata ataaatccct tggggctttc
    10201 cgaactatta ccttgttgca caggataggg aagaaaattg ggaataactg tctgttgggt
    10261 agaaagcact tcaatattta cgcctccggt atgaggaatg gctatagatg tacaaaccac
    10321 aggcaattca ggcatacctg tttccataag agttccggaa agaggtagta ttattttatg
    10381 ataaactgct cctccgtaag tttcttcctg catagaatat tctggcagag taaaatttac
    10441 ttgcagagag gaactatttt tagctcccaa agtaaaagct gaatttagat tagccggcag
    10501 attatcatta atattagcaa aaacgatgca acccgctaat aatatgagta ttaaggcaat
    10561 acttctcttc attggacttc cttataatgt gtaggatggt tttctatata ttagagcaaa
    10621 tattattcca aagaaaatca aagattatag taaagcagca tttcgtgagg gtgcggacga
    10681 gcagaaactg cttcattttc tttgagtttt aactctatat gactttctat gagttcctcg
    10741 ttaaaaacat caccttccag taaaaattga tgatcttctt tcagagcttg cattgcttct
    10801 ttaagatcag ttggaataga gagcaactgt gcttttttat cttcactcca ggcaaaaaca
    10861 ttatcatcaa aaggacccag attatatttg gctggatcaa tctgtttttt aattccatcc
    10921 aagccagcca ataaaattgc gctcattgcc aaataaggat tgcaggttcc atcaccggtt
    10981 ctaaattcaa agcgttttgt ttcaggagta ttagcatatt tgggaattcg gatagcggca
    11041 ctacgattgg caagaccata aaaaagtttt acgggtgctt caaaacctgg aagcaggcgt
    11101 ttaaaactat tggttgaagg attagtgaaa gcaactaaag cacggccgtg aattaaaatt
    11161 ccccctataa accaaattgc ttcttcggag agatctgcat aaccaccctt tttataaaac
    11221 aaatttttcc cgtttttatg caacataata tgaaaatgca ttccatttcc tgcatgtcca
    11281 aaaaccggtt tgggcataaa tgtagctgta agtccatatt gcaaagcagt tttacgaatg
    11341 atatccttca taatcattac atcatcacaa attttaggaa aggcaagcag ttctgtttca
    11401 atttcttcct gagcggaaat accaacttca tgatgatgat agcgaacttt aatatcctgt
    11461 tcttcaatca attccacaat tttttgccga atttcgtaaa attgatcaaa aggtgtatct
    11521 atatgatacc ctttaacacc ttgtaaactt aaggaaccag ggtcttcatt atcatccgga
    11581 agcgcttctt tacattcact ggaggtaata ttataaccag cagaaaagga atcggaacaa
    11641 tactgaactg aatcaaaaag atgaaattcc agttccggaa tccaggtaga catatctgca
    11701 attcctgttg aaagtaaata gttatgcgct ctaagagcaa cactgcgagg gtcttttttt
    11761 acccctaaac gggtttctgc atcgcaaatg gaacaaatca ttctcagggt gggggtctca
    11821 taaaaggact ccagatgagc tgttttggaa tctggcatta aaaccatatc tccactttcc
    11881 acacttctca ttccaggaat ggatgaacca tcaaaaggta ttccaacttc cataacttca
    11941 gcaatattac gagccggaaa tgtaatatga taccatttac catccagacc gcaatatttc
    12001 aggtctacgg cttttacttc atttgccaga ataaggtgtt gtaattcctt caattccatt
    12061 ttgttctcct tattatttta attttatatt tttaatttat gtaaagtcag gagtcgtcac
    12121 tgccgaaaca aattttactc ttaccacaaa ctcatttggc agttaaggac tcgtaacgac
    12181 ttaaaaagat aaaacacaga taactaaatc aactaatatc attttttgaa agataagcca
    12241 cttatcattc ctgcgaaaac aggaatccct atatgataaa atatatttct gcattttcaa
    12301 tctgaaatat atataagctc gtcataaatc atagatacca taaaagttat tgcacaatca
    12361 gaagttatca aggtgtaaat aactcctaac ttacctttcc ctaaattata atcttttatc
    12421 tgcgtaatcc gcatagtcag tgagagtcaa cttttatatc cgtaccccca taatttgcat
    12481 tacaaaattt accggcggaa taataatttt ccctataata ttccaaccca acaaagttgt
    12541 aatgataata aacgcaaaca aatacatcat tcctttgcgt tcttgcattg tccatttata
    12601 atatgcctga tcggttaaaa acattcctat aactttggaa ccgtctaaag ggggcaccgg
    12661 aatcaggtta aaaaaggcaa gcaagagatt gagatagatc acaaaaaaac aaatatattt
    12721 aattacaacc gaaaaaccgc acaaatgaaa gatcaaggca aagaaaattg ccaccagaaa
    12781 attggataga ggtcccgcta atcctgttaa gccagaatcc ctttttaagt tcttaaaatt
    12841 atagggattg aaaggcaccg gcttggcata accatatata aaaccgcagg ttaaatataa
    12901 aaacaacggc agaattacag ttccaaacca gtcaatatgt tttaatggat tcagagttaa
    12961 gcgtccggct cttttagcag aatcatcacc cagccagtat gccattaaag agtggctgaa
    13021 ttcatgaata ataatgctgt aaaaaaccag aaaaattata acagcattta ccagcagttt
    13081 tataatattg ggactgatca tatcttcttt atctttatgt aattacgctt tcccgcacga
    13141 ataatatctc catctttaag attaactaca gcatcaaaag cagtaatttt ctctccgttt
    13201 agggagacag ctccgccttg aatgagacgt tttgcttcac cgtttgtaga gcatattccg
    13261 ctatccacca ataaattaga cagtttacag ctgtttttgc ttaccacaaa ttcaggaata
    13321 tcattcggca cttctctcag ggaaaattgt ttttcaaagg catattccgc ttcttttgct
    13381 tcttctgcgc tatgatataa agtaacaatt tccctggcta agcgtttttt aattagcatc
    13441 gggttagttc cggattccag ttcttttttt attttttcaa tttcttcttc cggcatagtg
    13501 gtggcataat agaaatagtt caaaataata ttatccggaa tggacatcac tttaccgtat
    13561 ttttctgtgg gtggatcaaa tacggcaata tagttattaa gagatttgcc cattttattt
    13621 acgccatccg tgccaatcaa aatgggggat aaaatagcca cttgttcttc catcccaaaa
    13681 tgcctctgca tatctctacc acaaagaata ttaaattttt gttctgttgc tcccaattct
    13741 acatcgctat taacagcaac ggaatcataa ccctgcaaaa caggatacaa aatttcatgc
    13801 a
//
LOCUS       DAOPFL010000037        13585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5292_length_13585_cov_103.340576, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000037 DAOPFL010000000
VERSION     DAOPFL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5292_length_13585_cov_103.340576"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1562
                     /gene="tilS"
                     /locus_tag="PLG69_04125"
     CDS             114..1562
                     /gene="tilS"
                     /locus_tag="PLG69_04125"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPI42730.1"
                     /translation="MTIAEALKLLEKNIKENELIHNGDKLLLGCSGGADSNAMLYLFS
                     RLRVPLNLTLLVIHINHQIRGAESDADEDFVKQLCQDLNIPVIIRKIVVPETGNLENQ
                     ARKLRFEVFNKILESYNFDKILLAHQKEDQAETVMLNFLRGAGISGMAGIKGITGKVV
                     HPLLCFSRKELEEVLKQAGISWRTDSSNLETKFRRNLLRNEIMPQLEKEFNPALKSHL
                     FFLAELFSQAETIFKQRSKTQFRRLCIDIQPGKVVFELTPLLKLSPIERYYLFRSAFH
                     YVCGSENDFFAVHFQAIENILNGKGSKCLNLPHKITVIKQYAEVIVQIETEKEVKETE
                     PLVIDVDRTMAVYGNYRFNFKYLRVLPKEQTGDELESRVILDADKINIPFIIRFRQPG
                     DQFVPFGMTQLKRLKEFFIDEKVPKYERDLIPVFDDGEKLFWIVGYRLDNRVRYDENT
                     TRYLQITATNISVKSRKAILKKKRGNNESDEL"
     gene            1543..2091
                     /gene="hpt"
                     /locus_tag="PLG69_04130"
     CDS             1543..2091
                     /gene="hpt"
                     /locus_tag="PLG69_04130"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPI42731.1"
                     /translation="MNQMNCDISAVLFDEYQIQTRIRELGLEISNSYKNSTPVMIGIL
                     KGGFIFLADLVRSITIPVEVDFLAISSYGTKTSSSGVVKIRKDIDIDITGRDVIVVED
                     IVDTGLSLAYIKDYIWQHKPSSLKTCVLLDKPEAHKTDVTFDYIGYSIKNEFVVGYGL
                     DFAEKYRNLPFIGILKEELYKC"
     gene            2085..4319
                     /gene="ftsH"
                     /locus_tag="PLG69_04135"
     CDS             2085..4319
                     /gene="ftsH"
                     /locus_tag="PLG69_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPI42732.1"
                     /translation="MLKYILIALCLITFSALNAVPADTLITPQQTIPETGKAIMQFST
                     LMNWFILALLIISVISIIRMIIVKKRTLNRKDKPNPNPNLRNPQSPKNAPPKKSPFSL
                     PLIIMLILLIVIIFHTYNSSGTAITKESYSNFMTRVANGQITQVQFAEKDIFYTDIAN
                     KKYSTTMPYENPQLVDSLVALGIKVSATKPSRWAGVISYLLPFLLLIIFWVFFIRGMN
                     AQNAKAFSFGKSKARLYEASKTGITFKDVAGVDEAKEELQEIVEFLKDPKKFQRLGGR
                     IPRGVLLVGQPGTGKTLLAKAVSGEAGVPFFSISGSDFVEMFVGVGAARVRDLFDQAK
                     KNAPCITFIDEIDAVGRHRGSGLGGGHDEREQTLNQLLVEMDGFEPNEAVIIIAATNR
                     PDILDPALLRPGRFDRRVVVDLPDIKGRTEILKVHTAKVPLANDVHLELIARGTPGFS
                     GADLANLVNEAALIAASKNKTQIQMEDFEEAKDKLLLGKEKKSRVIPEEDKKLTAYHE
                     IGHVLTSVYLDKVEPVHKVSIIPRGFTGGATHYLMTDKTNYSKSYLLQMLVTLLGGRA
                     AEEVVFQEYTTGAGNDLERCTDIAKKMVCSWGMSDKIGPITIGKEQGEIFLGKELVSH
                     DIFSDETSQLVDREIRDIVSNAYSQAISLLNEHRELMDIMAKELQEKETLGTDEIFNL
                     ILENCREEERELVEEKYKKALELRFEHSEKTTSQPTETTETAETTETVEQAENKDNKS
                     EETA"
     gene            4320..4850
                     /gene="hslV"
                     /locus_tag="PLG69_04140"
     CDS             4320..4850
                     /gene="hslV"
                     /locus_tag="PLG69_04140"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPI42733.1"
                     /translation="MIMHGTTIIGIHRNGKTAICGDGQVTMGDAIVKGKAQKIRKIYD
                     GKVIVGFAGATADAFSLLERFEEKLKTNKGNLKKAAIDLAKDWRQDRILRRLEAMLIA
                     GDKDSILLINGAGDVLEPDDNIIAIGSGGNYALAAARALAHKTKLGASEIAVEALNIA
                     SGICIYTNNNYIVEEI"
     gene            4852..6216
                     /gene="hslU"
                     /locus_tag="PLG69_04145"
     CDS             4852..6216
                     /gene="hslU"
                     /locus_tag="PLG69_04145"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPI42734.1"
                     /translation="MLINEYLTPKRIVEELDKYIIGQDQAKRCVAIALRNRWRRQQIS
                     GDIKGEIMPNNIILIGPTGIGKTEIARRISRLTNAPFIKVEASKFTEVGYVGRDVESM
                     VRELMNYAVSQVREAMIEEVRPEAEKNATEMILDILLPRSKKMKQNANNTDTPEYERY
                     LRSREKMRKKLLSGSLNEKEIEVEPETDNLPNFEIFPGPGLELLDFDISEMISNIFQG
                     KGTPRKVKTTVHSAWKLYVENEINRLISKDKLVETAKMAVEENGIIFIDEIDKIANTD
                     KQVGIDVSRSGVQRDLLPIVEGSKVPTKYGMIDTSHILFIAAGAFNTAKPSDLIPELQ
                     GRFPIRVELNSLTQKDFERILTEPENSLTKQYQAIFRSEGVELKFMPEAIREIARFAA
                     LANEKMEDIGARRLHTVMNKLLDDFLFEMPSETLKSVKITKKMVTEHLSPVIESEDLS
                     RFIL"
     gene            6219..7529
                     /locus_tag="PLG69_04150"
     CDS             6219..7529
                     /locus_tag="PLG69_04150"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPI42735.1"
                     /translation="MVINKIKEIFVSLTIRNYRIFFIGQGISLIGTWIQRTTMGWFVY
                     RLTGSAFLLGLVSFLSMIPSVFVSPFIGAWADRLNRHRTMIHTQIAFCLQTGILALLV
                     LTRVINKQVWFPILILSFIQGIIDAVDAPIRQNFLMDLVSSRTLLPNAIATNSAMFNG
                     ARLIGPAIGGFLIMMFSEGVCFAINAISYIPVIISLCFIKISYPPVPPQKEPTLKKIF
                     SGWKYSWENMPIRFLLSNLAVFTVFGMSFSTLMPIFAKDILHGTSATQGLLMSSMGVG
                     ALCGSFFLASRKNIRGMPSRLVYLCLTFSIACILFSLSGSIPLSMFFMVFLGLAGMMT
                     MATTNTLIQSISSLEMRGRVISLYTMASVSMSPLGSLLMGTLSSRIGARFTLIVCGLV
                     LLLWSLNGLRIVPKFLRGVLRMLIISDNTDVYRQRVLAPEISAR"
     gene            7526..8215
                     /locus_tag="PLG69_04155"
     CDS             7526..8215
                     /locus_tag="PLG69_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPI42736.1"
                     /translation="MKLFYYLGKREPDYRTLMHLQKDFMLEGIKIPVSRLVVAEQTHS
                     KEVHICREIDCGAGIGNKPQIPLADGLITNIPYQYLLIRTADCYPILLMDSRRNVVAG
                     LHCGREGTQKNIVGEAIRKMKSHFYCQLTDIIAIVGAGICHKHYEVSQELFDNFNRAL
                     RKMGLEPDLTQNRHLNLRKILNAQLIKEGIPEINIENVNDCTFESSNYHSYRREKTNN
                     RQINIVGILYG"
     gene            8208..9500
                     /locus_tag="PLG69_04160"
     CDS             8208..9500
                     /locus_tag="PLG69_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPI42737.1"
                     /translation="MAKTYVNKEGNLILEIREEPLSAWLTIKKTDFLIDENEILALIE
                     EAGIKSGFDEAIDYICKHSLEKEFEVPFPIAMCNKKEVTSMLRYNFNPDLLSRPENGI
                     NISTLEKLKVFRSGDVVAEYSSNIFAQGGSIYDIFGNLLDANSVDTEQAKALAGDNIA
                     YNVQNKQFSALVDGFPYLDENGCICLLDRVLLNGNEIPPETKVKCPINLIIEGSITYA
                     DIHCEADISVQGDIQFSTINCAKNMFIAGDIISSNRKGIIVWGNLECRSILNSYVLCL
                     NNIHFTDKIENSTVVCDGEIIGDLNSSEICGGLTQAGESITVFQTGSYDRTKTEIEIA
                     LCPFYRNLLLLLTRELIHLKEDKELNATTIAELENRIEKYERALDEKLNQILKSEKQK
                     PKTIKVHDVIRPPVVFRILKHSYEVNRQEKGLEFEEKD"
     gene            9629..12667
                     /locus_tag="PLG69_04165"
     CDS             9629..12667
                     /locus_tag="PLG69_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPI42738.1"
                     /translation="MCYVYSNDLWLVPFTGGIPRRITNTTSYEWNPQFSPDGKMLAFS
                     SDRSGQNLIYIMPATGGEAKAVNYESFRISEWFSDSQNLLCTKYSPRWGLSFYKVPID
                     GSRPILLAEIGSSFATLSPDNKQIVFQKGGYSRREAYRGSGNGELWLIDLPTKKYTKL
                     TNTELSELYPRFSQTGALYYCASDGNCFQINKVNKLSFRKPEKITNLKPFSARRISLA
                     RNNDRLVFEYFNTIYKYDPTLKTGEKVSPLFIDLCEDEWQENIVRSYENTTIDDYTIS
                     TDELLVGFTHNYDSFFAPVKGGEAKQISFDHGSIFGLQFLDKRQMVISKLDNGIVKLF
                     TATADSVITLSPVDWFGADSLCVTELYKDKNGRWYFKYDDYYRHQKIAIADSGFVNIR
                     PVNTPWAVTTNFAFTEDGRYAVYGTLREDNYVKELYLYDVQADSSKRLLADNGWWQNL
                     FWTSDNKSILLTRNNDIYRLDLVPRDEFELDTDPWDEILHPEKSKADSTNEERQNEEN
                     GIQEIESTWKEKKEEKPKPLEIVWEGLEKRFYPVITAKDNYNFVLDIISDSTFYYIEN
                     NGQQEKNSILKKANIYGKNQKEVFNFGRNAGYYNKVNNTVYYLINQELKSYNLDTSAR
                     KDIKIKYDYKYDKALLNLRVFEEVWCAFGNNFYDPAMHNVPWKELFQLYRPYVEKAKD
                     IYDVAFIIEEMIGDVNASHTGFYPREEYKPFTNPVAWLGADFDYKDILAEGIRLERIY
                     PTSRLSSFYHLQDGCILTHIDEVKITAHTSLDSLLAGKIGKKIKLTILQEGNITEAVI
                     TGLSYSQMSELKYDYQIAKNKKLVDELSSGKLGYIHIPAMGENDWQKFYRDFVVDNFD
                     KDALVIDVRGNYGGHIHDKLITLLQKKKYAYTTSRRYSRVMCYEPHNIWDKPSIVLVN
                     EDSFSDGEIFPTVYQELKLGKVVGTPSSGAVIGTWHYYLQDGSSMRMPGTGWYKLDGT
                     NMEGTGVQPDIVVENLPEDIIAERDPQLIRAVKELMKELK"
     gene            12671..13546
                     /locus_tag="PLG69_04170"
     CDS             12671..13546
                     /locus_tag="PLG69_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI42739.1"
                     /translation="MNLNEINNLIQRANALLKTNWLQAVYILQEALKEEPDNLSLLVN
                     LGDIYLERQLYERALSYYQKAISLLPDNPQLLFLIGTCYFSLGEYRIANSYYNRIPDP
                     PPEILYNIALSYAFLGSYQESIDIIHKILKVMDDNPFIYFLLIEQYVRIQNYDRAYEI
                     ILTAENKFGKHRQLLLLSALVYGKKGIWLKSYHCFVEYESLGEITNPDHLMAYANAAV
                     NIGMNDRAIELLQRAQEINPYINAVYEELIRLYLKKNDYKNAKKVLNTAKRYISRFSP
                     VLYLFKERLENPHLE"
BASE COUNT         4390 a         2339 c         2859 g         3997 t
ORIGIN      
        1 accgagatat tttttacaaa gagaaaatcc gggttaaaaa aaagcagttg acattgaaag
       61 caagattaaa aaagatagta aaatagatta atgaggggag aaaaaaataa gtaatgacga
      121 ttgccgaagc attaaaactg ctggaaaaaa acatcaaaga gaatgagctt attcataatg
      181 gagataagct tttactggga tgttctggag gtgcggactc caatgctatg ctctatcttt
      241 tttcacgact tagagttccg ctgaatttaa ctttattagt tattcatatc aatcaccaaa
      301 ttcggggtgc ggaaagtgat gcagatgagg actttgtgaa acagctttgt caagatttaa
      361 atatcccggt aattattcgg aagatagtag ttcctgaaac gggtaatctg gaaaatcagg
      421 caagaaaatt gcgctttgag gtttttaata aaattttaga gagttataat tttgataaaa
      481 tcctgttggc acatcaaaaa gaagaccaag cagagacggt gatgctaaat tttttacgag
      541 gagcaggaat aagtggaatg gcaggaatta aagggataac aggaaaagtt gtccatccgc
      601 tattatgttt cagcagaaag gaattggaag aggttcttaa gcaggcggga atttcctggc
      661 gaacggattc cagtaatctg gagacaaaat ttcggagaaa tcttttgcgt aatgagatta
      721 tgccccaatt agaaaaggaa ttcaatccag cattaaaatc gcatctcttt tttttagcgg
      781 aacttttttc acaggcagag acaattttta agcaaaggag taaaactcaa ttccgacgct
      841 tatgtataga cattcaaccg ggaaaagttg tttttgaact taccccttta ttgaaactaa
      901 gcccaattga aaggtattat ttgtttcgca gtgcttttca ctatgtttgc ggaagtgaaa
      961 acgatttttt tgcggttcac tttcaggcaa ttgaaaatat attgaatggg aaaggcagca
     1021 aatgtctgaa cttgcctcat aaaataaccg ttatcaaaca atatgctgaa gttatagtgc
     1081 aaatagaaac ggaaaaagaa gtaaaagaaa ctgaaccatt ggttattgat gttgatcgca
     1141 ctatggcagt ttatggaaat tatcgtttta attttaaata cttacgtgtg ttgcccaaag
     1201 agcaaacagg ggatgaattg gaaagccggg ttattcttga tgcggataaa ataaatattc
     1261 cttttattat tcgttttcgc caaccgggtg accagtttgt tcccttcgga atgacacagt
     1321 taaaacggct gaaagagttt tttattgacg aaaaagtgcc aaaatatgag agggatttaa
     1381 ttcccgtttt tgacgacggg gaaaagttat tttggattgt gggataccgt ttggataatc
     1441 gggttcgcta tgatgaaaac acaacccgct atttgcaaat aacggcaacg aatatttcag
     1501 taaaatcccg aaaagcaatc ctgaaaaaga aaagaggaaa caatgaatca gatgaattgt
     1561 gatatttccg ctgttctttt tgacgaatac caaattcaaa cccgtattcg cgaactgggc
     1621 ttggaaattt ctaacagcta taaaaacagc acacctgtga tgataggcat tttaaaaggt
     1681 ggtttcatct ttttagctga tttggtgcgc agtataacta tccctgtgga agtggatttt
     1741 ctggctattt ccagttatgg aactaaaacc agcagcagtg gagtggtaaa aattcggaag
     1801 gatatagata ttgacattac aggcagggat gtgattgtag ttgaagatat tgtggatacc
     1861 ggtctttctt tggcatatat caaggattat atttggcagc ataaaccttc cagcttgaag
     1921 acctgtgtgc ttttggataa gccggaagca cataagacgg atgtgacctt tgattatatc
     1981 ggttattcca ttaaaaatga atttgtggtc ggttatggac ttgactttgc agaaaaatac
     2041 cgtaacctac cctttatcgg aattttgaaa gaagagcttt ataaatgcta aaatacatac
     2101 ttattgcctt atgcctgata acattcagtg ctcttaatgc tgttcccgca gataccttaa
     2161 ttactccaca gcaaacaatt ccggaaacgg gaaaagcgat tatgcaattt tccacgctga
     2221 tgaactggtt tattttggca ctgttgataa tatcggtaat cagtatcatc agaatgataa
     2281 tcgtaaaaaa aagaaccctt aacagaaagg ataaacccaa tcctaatccc aatttacgca
     2341 atccgcaaag cccgaaaaat gctcctccta agaaatcgcc tttctcgctg cctttaatta
     2401 taatgttaat tcttttgata gttattatat ttcataccta caacagcagt ggaactgcca
     2461 tcaccaaaga gagttacagc aattttatga cgcgggttgc taatggccag ataacacagg
     2521 tgcaatttgc ggagaaggac atcttttata ccgatatagc aaataaaaaa tactctacta
     2581 ctatgcctta tgaaaatcct caactggtgg attcacttgt tgccttggga attaaggtta
     2641 gcgctacgaa accctctcgt tgggctggag ttatatctta tcttttgcca tttcttttgc
     2701 tgattatctt ctgggtgttt ttcatccggg gaatgaatgc tcaaaatgcc aaagcattca
     2761 gtttcggaaa aagcaaagcc cgcttatacg aagccagtaa aacgggaatc actttcaaag
     2821 atgtagccgg agtagatgaa gcaaaagagg aactgcagga aattgtggaa tttttgaaag
     2881 acccgaaaaa gtttcaacgc ttaggtggaa gaataccgcg tggagtttta cttgtagggc
     2941 aacctggaac aggcaaaacc ctgcttgcta aagctgtttc cggagaggca ggagttcctt
     3001 tcttttctat cagcggttcg gactttgtgg aaatgtttgt tggcgttgga gcagccagag
     3061 tgcgtgacct ttttgaccaa gctaagaaaa atgcaccctg tattactttt attgatgaaa
     3121 ttgatgctgt tggcaggcat cggggaagcg gtttgggtgg tggacacgat gaacgcgaac
     3181 aaaccttaaa tcaactcttg gtagaaatgg atggttttga acccaacgaa gcggtaatca
     3241 ttattgcagc tacaaaccgt cccgatattc ttgacccggc attattgcgt ccgggaaggt
     3301 ttgacagaag agtggtagtt gatttacccg atattaaagg tagaacggaa attttgaagg
     3361 tgcatacagc taaagtccct ctggcaaacg atgtccatct ggaattgata gccagaggaa
     3421 ctcccggttt tagtggtgcg gatttagcta atttggtgaa tgaagcggct ctgattgcag
     3481 ccagtaaaaa caaaacccaa atccagatgg aggactttga agaagcgaaa gataagcttc
     3541 ttttgggtaa agaaaagaag agcagagtta ttcctgaaga agataaaaag cttactgctt
     3601 atcacgaaat aggccatgtg cttacatccg tgtatctgga taaggttgag cccgtgcata
     3661 aggtctctat tattcccagg ggttttacag gtggtgcaac gcattatctg atgaccgata
     3721 aaaccaacta ttccaaaagt tatttactgc aaatgctggt aacactttta ggaggaagag
     3781 cagccgaaga agttgttttt caagaatata ctaccggagc aggtaacgat ttggaacgct
     3841 gtaccgatat agctaaaaaa atggtttgtt cctggggtat gagtgataag attggtccta
     3901 taactatcgg caaggagcag ggagaaatat tcttgggtaa agaactcgtt tctcacgata
     3961 ttttcagtga tgagacctct caactggttg acagagaaat acgggatatt gttagtaatg
     4021 cttattctca ggcaatttct cttttgaacg agcaccggga attgatggat attatggcta
     4081 aagaactgca ggaaaaggaa accttgggca ctgatgaaat ttttaattta attttggaaa
     4141 attgccgtga agaagaacga gaattggtag aagaaaaata taaaaaagcc ctggaactac
     4201 gctttgaaca cagtgaaaaa acaacttccc aaccaaccga aaccacggaa accgctgaaa
     4261 ctacggaaac cgttgaacaa gcagaaaaca aggataataa atctgaggaa acagcataaa
     4321 tgataatgca cggtactact ataataggca ttcaccgcaa tggcaaaaca gcaatttgcg
     4381 gtgatggaca agtaactatg ggcgatgcta ttgtaaaagg taaagcccag aaaatacgca
     4441 aaatttatga tggaaaggta attgttggct ttgccggtgc tactgccgat gctttcagtt
     4501 tactggaaag atttgaagaa aaactgaaaa ccaataaggg taatttgaaa aaagcagcta
     4561 ttgacctggc taaggactgg cgtcaggata gaatcttgag acgcctggaa gcaatgctta
     4621 ttgcgggtga taaagattcc attctgctta taaacggagc aggcgatgtt ttagagcccg
     4681 atgataatat tatagccatt ggcagtggcg gaaattatgc tttggcagca gctcgcgctt
     4741 tagcacataa aaccaaactc ggagcaagcg aaattgccgt agaggcactg aatattgctt
     4801 caggaatatg catttatact aataataact atattgtaga ggaaatataa attgttaatt
     4861 aatgaatact taactcctaa acggattgtt gaggaactgg ataaatatat tattggccaa
     4921 gaccaggcaa aacgctgtgt agcaatcgct ttacgcaatc ggtggcggcg tcagcaaatt
     4981 tccggtgaca tcaagggtga aattatgcct aataatatca tcctgatagg acctaccggt
     5041 attggtaaga cagaaattgc cagaagaatt tcccgtttaa caaatgctcc cttcatcaaa
     5101 gtggaggcat ccaaatttac cgaagtaggt tatgtaggac gcgatgtaga atcaatggtg
     5161 cgtgaactga tgaattatgc ggttagccaa gtgcgcgaag caatgattga agaggtgcgt
     5221 cccgaagcgg aaaaaaatgc tacggaaatg attttagata tattgcttcc gcgcagtaaa
     5281 aagatgaagc aaaatgccaa taacaccgat acacctgaat atgaacgcta tttacgcagt
     5341 cgggaaaaaa tgcgcaaaaa actgctttcc ggtagcttga atgaaaagga aattgaagtt
     5401 gaacctgaaa cagataacct tcccaatttt gagatttttc cgggaccggg actggaatta
     5461 ctggattttg acatcagcga aatgatttcc aatatttttc aagggaaagg aacaccaagg
     5521 aaagtcaaaa ccactgttca tagtgcctgg aaactctatg tggaaaacga aattaaccgt
     5581 ctgataagta aagacaaact tgttgaaaca gctaagatgg cagtggaaga aaacggtatt
     5641 attttcattg acgaaattga taaaatagcc aatactgata aacaggtagg tattgatgtt
     5701 tcgcgtagtg gcgtccaaag agacctgctt cctatagttg aaggcagtaa agttcctacc
     5761 aaatatggaa tgattgatac ttcgcatatc ttgtttattg cagcaggcgc ttttaacaca
     5821 gcaaaaccat cagacctcat tccggaattg caaggcaggt ttcctatccg ggtggaattg
     5881 aatagtttaa cacagaagga ttttgaacgc attttaacag aaccggaaaa ttctttaact
     5941 aaacagtatc aggcaatttt ccggagtgaa ggagtagaac tgaaatttat gccggaagcg
     6001 attcgggaaa ttgcccgttt tgctgcttta gccaatgaaa aaatggaaga tattggagct
     6061 cggcgtttgc atacagtgat gaataaacta ttggatgatt ttctgtttga aatgccttcc
     6121 gagacactaa aaagcgttaa aatcacgaaa aaaatggtaa ccgagcacct tagtcccgta
     6181 attgaaagtg aggacctttc ccgatttatc ctatagcgat ggtaatcaat aaaataaaag
     6241 aaatttttgt ctctttaact atccgcaact accgcatttt ttttataggg cagggaattt
     6301 ctttaatcgg aacttggata cagcgaacaa ctatgggctg gttcgtttat cgcttaaccg
     6361 gttctgcttt tttacttggc ttagtaagtt ttctctcaat gattccttcg gtttttgtaa
     6421 gtccctttat aggtgcttgg gcagaccgtt taaaccgtca tcggacaatg attcatacgc
     6481 aaattgcttt ttgcctgcaa acaggtattt tggcattatt ggttttaacc agagtaatca
     6541 ataagcaggt ttggtttcca attttgattt tatcttttat tcaaggaatt attgatgctg
     6601 tggatgctcc tattcgtcag aactttttaa tggaccttgt ttcttccaga acattattgc
     6661 ccaatgctat agcaaccaat tccgctatgt ttaatggagc gcgtttgatt ggacctgcta
     6721 ttggaggttt tttaataatg atgttcagtg agggggtttg ttttgctatt aatgcaattt
     6781 cttatatacc tgttattatt tcattgtgtt tcataaagat aagctatcct cctgttcctc
     6841 ctcagaaaga accgacgctg aagaaaatat tttcggggtg gaagtattcc tgggagaata
     6901 tgcctattcg ctttttgctt agtaaccttg ctgttttcac tgttttcggg atgtcttttt
     6961 ccactttgat gcctattttt gccaaggata ttttacacgg cacttcggca acgcagggtt
     7021 tgctaatgtc ttcaatgggt gtaggagctt tatgtggttc attcttttta gcttcgcgca
     7081 aaaatattcg gggaatgcca agtcgtttag tttatttatg tttaactttc agtattgcct
     7141 gtatattgtt ttcgctttcc gggtcaattc cgcttagtat gttctttatg gtgtttttag
     7201 gtttggcggg aatgatgaca atggctacta cgaatacttt aattcaaagt atttcttctc
     7261 tggaaatgcg aggaagagtt atttctcttt atacaatggc ttcagtgagt atgtctccat
     7321 taggaagttt gctaatggga actttatcca gcagaattgg agctcgcttc actttgattg
     7381 tgtgtggttt ggtattatta ttgtggtcat taaacggctt gaggatagtt ccgaaatttt
     7441 tgcgaggtgt cttaagaatg ttaattatta gcgataatac ggatgtttac aggcaaagag
     7501 ttttggcacc ggagatttct gcaagatgaa attgttctat tatttgggga aaagagaacc
     7561 tgactaccgc actttgatgc accttcaaaa ggattttatg ttggaaggaa taaaaattcc
     7621 cgtttcgcgt ttagttgttg ctgaacaaac acattccaaa gaagtgcaca tttgtcggga
     7681 aattgattgt ggagcaggca tcggtaataa gccccaaatt cctttagcag atggtttaat
     7741 taccaatata ccctatcaat atttacttat tcggactgcc gattgctatc caattttatt
     7801 aatggatagt cgcagaaatg tagttgcagg tcttcattgc ggtagagaag ggacacagaa
     7861 aaatatagtt ggagaagcaa taagaaagat gaaaagtcat ttttattgcc agttgaccga
     7921 tatcatagcc atagtaggtg ccggtatttg tcataaacat tatgaagtaa gccaggaact
     7981 ctttgataac tttaatagag ctctccgaaa aatgggattg gaaccggatt taacacaaaa
     8041 caggcatctt aacctcagga aaattctcaa tgctcaactt attaaagaag gaattccgga
     8101 aatcaatatt gaaaatgtta atgattgcac ttttgaaagt tccaattatc attcctatcg
     8161 tcgggaaaaa accaataacc gtcaaataaa tattgtgggt atcctttatg gctaaaactt
     8221 atgttaataa agaaggaaat ctgattttgg aaatccgcga ggaacctcta tctgcttggc
     8281 taacaatcaa aaaaacggac ttccttatag atgaaaacga aattcttgcc cttattgaag
     8341 aagcaggcat taaaagcgga tttgacgaag cgattgatta tatatgcaaa cattcactgg
     8401 aaaaggaatt tgaagttccc tttcctattg caatgtgcaa taaaaaagaa gttaccagta
     8461 tgttacgcta taactttaat cctgatttgc tttcccgtcc cgaaaacgga ataaatatta
     8521 gcactttgga gaaattgaag gtttttcgtt caggagatgt tgtagcagaa tattccagta
     8581 atatttttgc acaaggtggc agtatctatg atattttcgg aaacttactt gatgccaatt
     8641 cggtagatac ggaacaggca aaagctctgg caggtgataa tatagcttat aatgtccaaa
     8701 acaaacaatt ttccgctctt gtagacggct ttccctatct ggatgaaaat ggctgtatct
     8761 gcctgttgga tagggtattg ttaaacggaa atgaaatccc accggaaaca aaagttaaat
     8821 gccctatcaa tttgattatt gaaggaagca taacttatgc cgatattcat tgtgaggcag
     8881 atataagcgt tcagggcgat attcaattct caactattaa ctgtgctaag aatatgttta
     8941 tagcagggga tataatttcc tcaaacagga aaggaattat cgtttgggga aatctggaat
     9001 gccgtagtat tctcaattct tatgttcttt gcctgaataa catccatttc acggataaaa
     9061 ttgagaattc taccgttgtt tgtgacggag aaataatagg tgacttgaac agcagtgaga
     9121 tctgcggtgg cttaactcaa gcaggggaat caattactgt ttttcaaacc ggttcttatg
     9181 accggacaaa aaccgaaata gaaatagcac tttgcccctt ttatcgtaat ctgctattgc
     9241 ttttaaccag ggaacttatt catctgaaag aagataaaga attaaatgct accacaattg
     9301 cagaattgga aaaccgcatt gaaaaatatg aaagagcttt ggacgagaaa ctaaatcaaa
     9361 ttctgaaaag cgaaaaacaa aaaccgaaaa caataaaagt gcacgatgta atcagaccac
     9421 ctgttgtatt tcgcatctta aaacacagtt atgaagttaa cagacaggaa aaaggtcttg
     9481 aatttgagga aaaggactaa ggaaaatgat tagtgttaac agcaaaaaaa tcttggagct
     9541 attgtgcata ctgatttgcg cttgtcctct ggtagcacag gaggcatatt ttacgagtga
     9601 accggccata tctccagatg gagaaatagt gtgttatgta tatagtaatg acctatggct
     9661 tgttcctttt acaggaggaa ttcctcgtag aataaccaac acaacttctt atgaatggaa
     9721 tccccaattt agtcctgatg gcaaaatgct tgccttttct tctgaccgct caggacagaa
     9781 tctgatttat ataatgccag ctacgggagg agaggcaaaa gccgtaaatt atgagtcatt
     9841 cagaatttca gaatggttta gcgattccca aaatctttta tgtaccaaat attctccgcg
     9901 ttggggttta tctttctata aagttcctat tgatggttcc cgtcctattc tgcttgctga
     9961 aattggcagt tcctttgcta ctttaagtcc tgacaacaaa caaatcgtat ttcaaaaagg
    10021 tggctattcc agaagagaag catatcgcgg tagtggcaac ggagaacttt ggctaattga
    10081 tcttcctacg aaaaaatata ccaagcttac caatacggaa ctttcggaat tgtatccccg
    10141 tttttcgcag acaggagcat tatattattg tgcctccgat ggtaattgtt ttcagatcaa
    10201 caaggtgaat aaactatctt ttcgcaaacc cgaaaaaatt accaatctaa aaccctttag
    10261 cgctcgcaga atcagtttgg caagaaataa tgatcggctg gtttttgaat attttaatac
    10321 tatttacaaa tacgatccta ctctcaaaac gggagagaaa gtaagtccac tgttcataga
    10381 cctttgcgaa gacgaatggc aagaaaatat tgtccgcagt tatgaaaata ccactattga
    10441 cgattatact atctctacgg atgaattatt ggttggtttt acccataatt atgatagttt
    10501 ttttgctcca gttaaaggtg gagaggcaaa acagattagt tttgaccacg gttcaatatt
    10561 cggtctgcaa tttttagata aaagacagat ggtaataagc aagctggata atggaattgt
    10621 gaaacttttt acggcaacag cagattcggt tatcactctt tctcctgtgg attggtttgg
    10681 agctgatagt ttatgtgtta cagagcttta taaagataaa aacggacgct ggtatttcaa
    10741 atatgatgat tattacagac atcaaaaaat tgctattgcc gattcgggtt ttgttaatat
    10801 ccgtccagtt aatacacctt gggctgttac aaccaatttt gccttcactg aagacggtag
    10861 atatgcagtg tacggaactt tacgcgaaga taattatgtg aaagaattat atctgtatga
    10921 tgttcaggca gattccagta aacgcctttt ggctgataac ggttggtggc aaaatctttt
    10981 ctggacttcc gataataagt ccatcctctt aacccgcaat aatgatatct accgcttaga
    11041 tttggttccc agagatgaat ttgaattaga tacagaccct tgggacgaaa tcctgcatcc
    11101 ggaaaagagt aaagccgata gtactaatga agaaaggcaa aatgaggaaa atggcattca
    11161 ggaaattgaa agcacttgga aagagaaaaa agaagaaaaa cccaaacctt tggaaatcgt
    11221 ctgggaagga ttggaaaaac gcttttatcc tgttatcact gcaaaagata actacaattt
    11281 cgtattggac ataataagtg acagcacttt ctattatatt gaaaataatg gacagcagga
    11341 aaagaattcc atcctcaaaa aagccaatat ctacggaaaa aatcagaaag aagtgtttaa
    11401 tttcggtaga aatgcaggtt attacaataa agttaataat acagtgtatt accttattaa
    11461 tcaagaactg aagtcctaca atctggatac ttcagcccga aaagatataa aaattaaata
    11521 cgattacaag tatgataagg cacttttaaa tctgcgggtg tttgaagagg tatggtgcgc
    11581 ctttggcaat aacttttatg accctgctat gcataatgtt ccttggaaag aattgtttca
    11641 gctctaccgt ccttatgtag aaaaagcaaa ggatatctat gatgtggctt tcattataga
    11701 agaaatgatt ggagatgtaa acgcttccca tactggtttt taccccaggg aagaatataa
    11761 accctttacc aatcctgttg cctggctcgg agccgatttt gattataagg atattttagc
    11821 agaaggaata cgcttggaga gaatttatcc aacatcccgt ttgagcagtt tctatcatct
    11881 tcaagatggc tgtatcttaa ctcatattga tgaagttaaa attactgcac atacttcttt
    11941 agattcattg ctggcaggaa aaattgggaa aaagataaaa ctgactatcc tacaggaagg
    12001 aaatattacc gaagccgtaa ttacgggctt aagctattcc caaatgtctg aattgaaata
    12061 tgattatcag atagctaaga acaaaaaact ggtggatgaa ctctccagcg gaaaacttgg
    12121 atatatccat attcccgcta tgggagaaaa tgattggcaa aaattctacc gtgattttgt
    12181 agttgataat tttgataaag atgcactggt aattgatgtg cgtggaaact atggcggaca
    12241 tattcacgac aaacttatta ccctgctcca gaaaaagaaa tatgcctaca caacaagccg
    12301 tagatacagt cgtgttatgt gttacgaacc acataatatt tgggataaac caagcattgt
    12361 attagttaat gaggattcct tcagcgacgg agaaattttc cctactgtgt atcaggaatt
    12421 gaaattggga aaagttgtcg gaactccttc cagcggagca gttatcggca cctggcatta
    12481 ctatcttcag gatggttctt ctatgcgaat gccaggaacc ggatggtata aactggatgg
    12541 cacaaatatg gaaggaacag gtgttcagcc cgatatagta gtagaaaatt tacccgaaga
    12601 tattattgca gaacgcgacc ctcaactcat tcgtgccgtt aaagaactga tgaaagagct
    12661 aaagtaaatt atgaacttaa atgaaataaa taatcttatt caaagggcta atgccctgct
    12721 aaaaacaaac tggctgcaag ccgtctatat ccttcaggaa gcattgaaag aagaacccga
    12781 taatctttcc ctccttgtta accttgggga tatttatttg gaacgccaac tctatgaaag
    12841 agccctcagc tattatcaaa aagcaatttc cctgcttcca gataatcccc agttgctatt
    12901 tttgattggt acttgctatt tttccttagg ggaatataga attgctaata gctattataa
    12961 tcggattcct gacccacctc cggaaatcct ttataatatt gccttgtctt atgctttttt
    13021 gggttcatac caggaaagta ttgatataat tcataaaatt ttgaaagtga tggatgacaa
    13081 tccttttata tattttcttt tgattgaaca atatgtccgc atccaaaact acgaccgcgc
    13141 ttatgaaatc attttaactg ctgaaaataa gtttggtaaa catagacagt tgttgctcct
    13201 ttccgcattg gtttacggaa aaaagggtat ctggctgaag tcataccact gctttgtaga
    13261 atatgaatcc ttaggagaaa taaccaaccc cgaccatctt atggcttatg ccaatgcagc
    13321 tgtaaatatc ggaatgaacg accgtgctat tgaactttta caaagagctc aggaaataaa
    13381 tccctatatc aatgccgttt atgaggaact gattcgcctg tatctgaaaa aaaacgatta
    13441 taaaaacgct aaaaaagtcc ttaacaccgc taaaaggtat atttcccgtt tttcgcctgt
    13501 cctttatctc ttcaaagaac gactggaaaa cccgcatttg gaatgaaaaa tgaatttaaa
    13561 aaaggttgag agggttgaga gggtt
//
LOCUS       DAOPFL010000038        13510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5334_length_13510_cov_100.931996, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000038 DAOPFL010000000
VERSION     DAOPFL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13510
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5334_length_13510_cov_100.931996"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1220..2425)
                     /locus_tag="PLG69_04175"
     CDS             complement(1220..2425)
                     /locus_tag="PLG69_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42740.1"
                     /translation="MKRNLIFLFIPAIILVAGALFATESRMAGLGFPYGYIKDNSEIS
                     IYPGAINRYKTQVIGELKSPGSNPDWTLGANLPIKKCVGGIYLNTLTGINVDEMLYDS
                     ESPNLYSRGDLDLSKKIQLYLGFWDKWGIGFGMAIDNIKNDVGDNPNIYEKSSAYYFD
                     LSGGMSTDKCDVGLQVKYAGGSAENEVATNKDFNDEFYRFGVDLAGRYYLLQNQDVDV
                     MCLGKGYFNIGSKKIDIPNTENKPVEKTTDDNNFMIEVGAGVNYKLNDTNTIIFGFKP
                     IRFASESIEESKEGTNGSHKMSHSFIYVPEYNLAVESQITRWLTGRVGARQNYVFYSE
                     KDEPSGDGEYEDSEYQSEFVMNLGLGFNFGKFSIDAVLEHDFLHHGPEFIGGKTNGLA
                     SMISLTYQY"
     gene            2909..3943
                     /gene="mtnA"
                     /locus_tag="PLG69_04180"
     CDS             2909..3943
                     /gene="mtnA"
                     /locus_tag="PLG69_04180"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPI42741.1"
                     /translation="MQIDGKQYLSVWFENERVKMIDQNKLPFEFRIAEYSNYLEICDA
                     IREMTVRGAPAIGATGAYAIALAAQNAPEEKFRAYLRTARDELIATRPTAIDLRNGVN
                     YVYEQTIKFIPDYRHSRQVAILAAREFAKKSIDDCYQIGKIGSEIVPHGARILTHCNA
                     GALATVDWGTALAVIRIAHQQNKDIFVYVDETRPRFQGARLTAFELEQEGIPHTIITD
                     SASGFYFWKKEIDLVITGADRICLNGDIANKIGTYEKAVLAKEHKVPFYVAAPFSTFD
                     LATKEGADIPIEIRDEDEIKRINGTYLANPGSPALNPSFDITPAKLITGIITPKGIFK
                     PKDAARQVQV"
     gene            3940..5259
                     /locus_tag="PLG69_04185"
     CDS             3940..5259
                     /locus_tag="PLG69_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPI42742.1"
                     /translation="MIFDIVLEGGSIITMDDKNRILSQSCIAIKDGKIQAIFPIGSSS
                     YETKQKIDTHNCFIIPGLINMHSHLPMTYFRGLADDLPLNTWLQKYIWPLEAKLIKPQ
                     LVYDATLHGASEMIKNGISTTNDMYFCMNSIADACSQVGLRVFISEALIDNESSKEDR
                     DNTIGRRITQLKEEYKDNPLIDFTLAPHSIYACSVETLKTCAKIAKDNNILIHTHLSE
                     IREEVENCLKIHKLKPVEYLKELGLLEVRSIFAHGIWIAEEEMELLAEKGLSSIAICT
                     ESNLKLSSGFAPIKKYQEKGINLCLGTDGVASNNNLDLLTEMSVTAKLHKALNNDPTM
                     LPAQEAFAFVTINAAKALGKEKELGSLETGKIADIAVVSLQELENSPVYNPYSLLVYA
                     INSHSIRDMIIQGKIVMQNRQLCNVNEQQILDKATDYKEMILQATEK"
     gene            5256..5684
                     /gene="smpB"
                     /locus_tag="PLG69_04190"
     CDS             5256..5684
                     /gene="smpB"
                     /locus_tag="PLG69_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPI42743.1"
                     /translation="MKTIKNRRAEHDYFIIQTLEAGIALKGTEIKSIRAGKVNFKDSY
                     AKIEDGECWLHNLHISPWEKAAYFNHEAERKRKLLLHKHEIRRLKTKVEEQGMTLIPL
                     ELFINDEGLCKITLALAKGKKAYDKREVMRKKDLERDQEG"
     gene            5804..7279
                     /locus_tag="PLG69_04195"
     CDS             5804..7279
                     /locus_tag="PLG69_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPI42744.1"
                     /translation="MENQVTPLETHLNRITFFLNGEKITADIPAGISTLDWLHTQKQL
                     YGTKCSCNEGDCGACTVVIAYPFEGIITYQAITSCLYPAAKLHGKHLITIEGLGTPEK
                     LHTIQQALLTNHAIQCGYCTPGLVMSLFALFAMQPHPDREMTLSALEGNLCRCGTYPS
                     ILNAVDELATNQSVKDIVPAWCREVEKDLFHFAQKEAMLVTKTDIPQQVNCYLIPTTT
                     KQLFDLYTEHPESVFIAGGTDIMVQKNINRKEFPFLIDLTQIPELNRLYLQQDGLHIG
                     SAVTYNQLWESGIVKTDFPVLHNLISQIASRQIRNLGTLGGNIANASPVGDTIPLLMV
                     LNASLWLQSNLELRHLPLKDFFLGYHKTALQKGEIIKEIIIPPLTEDNYVKTIKSAKR
                     KSVDISSVITAVNIEHKNGQITNSALAIGGVAAVPLLSRKFPNLLKSKQIKALDPAVI
                     IKEVESEFEPLTDIRGTALFRRELIRNHLILYLQELQEEEK"
     gene            7276..9543
                     /locus_tag="PLG69_04200"
     CDS             7276..9543
                     /locus_tag="PLG69_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPI42745.1"
                     /translation="MNSETYHLSGTLHLCGKSHFIADEPPLQGELYAKFLFSPVAHSL
                     ITKLDISAAAKMPGVVKVITSADIPGVNQIGTVIKDEPLFPEKEIMYVGQPLAMVLAE
                     DEYIVELAVKKIILKFEELPAIFAPEDAYSKKQGYISERKIESGDIENALKNAPYTLK
                     GKTVTTGQEHFYLEGQRCLAVPFEGNRITLQTATQSTMEVQEVASRVLGIPANDVIIE
                     VPRLGGAFGGKERGATIWACMAALGAFLTQKPVLVKLTRDEDMHYTGKRHPFTSLWQV
                     GYDETGKILAYDLLLLANGGAYTDLSIAILERAMFHSDNAYFIPNIRIRGYACRTNLP
                     PNTAFRGFGAPQGIFVIESIMDKIAAQLELDPLEIRKRNAYQNGQLTPYGQEITQSQL
                     NNIFAKLADKANYALLRKEVAEFNKHNRRYKRGIGITPVKFGISFTTTLMNQGSALVW
                     VYIDGSVSVSTGGVEMGQELSTKVAIVVSRVLGIPVNKIRVESSNSQRIGNASPTAAS
                     TGSDINGNAARLAAEKIKDNLAHCAVRLIQQKYNQTIDIKDVVFKDNRVFGKEDPETC
                     ISFEELVRFAHSERVPLAAYGYYKTPGLWFDREKGLGHPFHYYVYGAGLAEVELDTLL
                     GEHKVRKLIIVHENGNSLNGAIDRGQIEGAVLQGIGWCTMEDLKYDAKGVYLSANPST
                     YKIPAIRDLPEKLVIEIIPSLTEEASVFGSKGIGEPPFIYGLSVFFALQSAFKEANPE
                     KELSFPATPESLIQR"
     gene            complement(9616..11823)
                     /locus_tag="PLG69_04205"
     CDS             complement(9616..11823)
                     /locus_tag="PLG69_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPI42746.1"
                     /translation="MKRTYIFIVILFLALCLNAQPWEMDNSVFNPSGVPSFSFSQPRF
                     MDLDSDGDFDFWLGNTNRPPLYLQNNGTATNPVFAPGPDLLSGIPYLASEIAVSADIN
                     GDGILDLVTGGFSGLHLFLNSGTNANPVFTESGGYFSGLNVGSNPVPDVADVDNDGDL
                     DLVVGLSENGSVIIYFNNGSAAVGNFSESNCQLIGDIGLYAYPVFCDFDSDGKQDILC
                     GRDTHGFVFYQNIGTATNPIWEENNTFFSGLGMSTYWNSPDLVDLNGDGLYDLVYGTA
                     AGPLQYYVHTGTAENPSWQQNTSLFGGVLDVGGASNPVFYDFDGDGDLDLISGNQLGY
                     VKFYRNTGTAYAPAWQEDNSYFANIHHSIYSAVTVGDVDADGLPDVILGDLNGGLYFY
                     HNTGTGFIEQTGVLPAVSVGGWSCPRLIDMDFDGDLDLVVGNEAGNLFYYQNNGTPYS
                     PLWELVNGFFGAIDVGSDCVPTFADVDEDSDLDMVTGNLFGEMQCFLRQRQLWVENTT
                     LFSGIETDQNAAPALVDLDHDGDFDLVLGDYDGTFKFFRNLKYSGSVLNPPQNPIFII
                     DGEVTVTWEEPLTGSTSPFEHYKIYLDGVYCGSTTENIWVFSGLDAGVAYLVCITAQY
                     VAGESLPVTLEFIITGNDDQIVQPITLTNYPNPFNPSTTISFTIPAGSKGTLEIYNVK
                     GQKVRVWNCLPSGEHKIVFNGCNEQGLPLPSGIYFGIMKTQNKVQIRKMVLIK"
     gene            12004..12177
                     /locus_tag="PLG69_04210"
     CDS             12004..12177
                     /locus_tag="PLG69_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42747.1"
                     /translation="MIKRFIASRILSNLHKGKPVLILGARQSGKTTLINLNNVIWETG
                     FVNSFFAEEREGF"
     gene            complement(12303..12500)
                     /locus_tag="PLG69_04215"
     CDS             complement(12303..12500)
                     /locus_tag="PLG69_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42748.1"
                     /translation="MVLQRDKEEKRIVLQRDKEEKRIVLQGEKEEKRIVLQGGKEVSF
                     TRRERGKENSFTKRESSNFVL"
     gene            12667..>13510
                     /locus_tag="PLG69_04220"
     CDS             12667..>13510
                     /locus_tag="PLG69_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPI42749.1"
                     /translation="MVGIVLSYTTLGIDAYQISVEADIGTGFNPMNIVGMASNAVKES
                     KDRVIAAIKNSGYQISTQHYTINLAPADLKKDSAALDLPIALAILQCNEIFRIIDYDT
                     IAMIGELSLDGFVRPVAGVLPIALAAKKDGINTLIVPLENAEEAAIIEGLNVIPVTSL
                     RECVSWLCKETEIMAANVDREKIFQVLNDFPVDMSDVKGQFQVKRALEVAAAGGHNVL
                     MIGPPGSGKTMLARRVPTILPELNLEEALEATKIHSVAGYSKNFRNGILTTRPFRSPH
                     HTISD"
BASE COUNT         4310 a         2595 c         2540 g         4065 t
ORIGIN      
        1 aagataatta attaaaggcg aagcagttaa cagaagggcg gtaaatgata atgttattcc
       61 cttttggtta atcgggattc ggtaactcca taatgaaata atattatttt tatattttat
      121 cttcttattg aagtaaaaat tattagtatc tgtcgttcct atggaactct ctctaaaaga
      181 aggagatatg ttgtttattt acactctgtt cgccgggttg aaacccgtcg ttaatacctg
      241 tcgttcctac ggtactcttt ctgataaaag gagatatgtt gtttttattt gctcttttcg
      301 ccgggttaaa acccgttgtt aatacctgtc gttcctacgg aactctttct aaaagaagga
      361 gatatgttgt ttttatttac tctttgcgcc gggttaaaac ccgttgttat tatctgtcgt
      421 tcctacggta ctcgttttta aagagaaagg tatattgtaa gttctttctc ttttttgggg
      481 ttaagcccaa atattaaaat cgggataaat ggggcaaaaa attctttatt caccagagca
      541 aatggatgtt tctaaaggaa gttataactt cagcatttac ttaattcaga atttacatag
      601 ccgtaaattg caacaaaaaa agtatttttt acgaaattac cgcagtccag gaaaaatact
      661 tttatgcagt tttggattcc tgcttgcact gaaatgataa agaatttttc ccctcaagac
      721 attacattat atactttata atcatcattt ttattataaa ctaaaaacaa ttccgctgcg
      781 catttcgctc tttcctaaaa ctatcaatct ataatttatc atacctttat caagcgttaa
      841 ttgttaacgc ttgattaaca cttttaacta aggatagata gtgaagtttt tctctaatga
      901 agaattaaat aactttcata ttgaaaaaaa ataaatatat tttttacctt ctatggattc
      961 cttctatctc aggaaggaca aaaacgcagg aatgacaaaa tgtgtattct tttgctatgt
     1021 tgacgcttgt tattccacac ctgttacgga atccatttta attaagtaga aacttgcttg
     1081 cgttggaatg gcaaaaagaa tggattcctg tttgcgcata aatctaatgc ttaaaaatat
     1141 tatctttatt tgaaattaat aggaagctgg agttcagaat accgatttgg tattgaactc
     1201 cagctcgtta aagggttttt tagtattgat aagttaaaga aatcattgag gcaagaccat
     1261 tggttttgcc accaatgaat tcaggtccgt gatgcagaaa atcgtgttcc agaacagcat
     1321 ctatgctgaa tttgccaaag ttgaagccca aaccaaggtt cattacaaat tcggactgat
     1381 attccgaatc ttcgtactcc ccatctccgc ttggctcatc tttttcggaa taaaatacat
     1441 aattctgacg agctcccacg cgtccagtca gccaacgggt aatttgtgat tccacagcca
     1501 gattgtattc cggaacatag ataaaactat gcgacatttt atgcgagccg ttagttccct
     1561 cttttgactc ttctatactt tcggaagcga aacgaatggg tttaaagcca aagatgatag
     1621 tattagtatc gttgagtttg taattaactc ctgctccaac ttcaatcata aaattgttat
     1681 catcagttgt tttctctaca ggtttatttt ccgtgtttgg aatatctatt ttcttactac
     1741 ctatattaaa atatccctta cccagacaca ttacatcaac atcctgattt tgcagtaaat
     1801 aatatctgcc tgcaagatca actccaaagc ggtagaattc atcattgaaa tccttattag
     1861 tagccacttc attctctgct gaaccacctg catatttaac ttgtaatccc acatcacatt
     1921 tgtctgtaga cataccaccg cttaagtcaa aataataagc actggacttt tcataaatat
     1981 taggattatc tccaacatca tttttgatat tgtctatagc cataccaaag ccgatacccc
     2041 atttgtccca aaaaccaagg tataattgaa tctttttgct gaggtccaaa tcaccgcggg
     2101 aatataaatt tggagattcg gaatcataca gcatttcatc tacattaata cctgtgaggg
     2161 tatttagata aattcccccc acacatttct tgataggaag gtttgctccc aaagtccaat
     2221 cggggtttga tccgggggac ttcaattctc caataacttg agttttataa cgattaatgg
     2281 caccaggata aattgaaatt tcagaattat ccttaatata accataagga aaaccgagac
     2341 ctgccattct gctctcggta gcaaataaag ctccagcaac taaaataatt gccggaatga
     2401 agagaaaaat aaggttgcgt ttcatttttt tacctcactt ttttattttc tctggctgat
     2461 ctaagccata atattttttc agtgattaaa agagaatttg gaaaatttat tactgacccc
     2521 atctccaact ttggcaattt atatatcatc tcgctaataa aagaaatatt tcttttattc
     2581 gggcacttct ggagttttgg aacaccccaa agttttgttt caggactcaa cttctgataa
     2641 agcataacat tatatactcc taatctgttc attatgaaca ataacaacaa gttaaaacct
     2701 ctttataata ttttaatcta taaccatctt tctggcaaga aatattttta atttccaaga
     2761 attacagaaa aaataagaaa gatgttgacg aaataaccgc catctacgga aaatgcattt
     2821 atgcagtttt agattcctgc tggttcagga atgataaagg tgaaaagtaa tactaatata
     2881 tacagcagtc ctaatatagg gagaataaat gcaaatagac ggaaaacaat acctttcggt
     2941 atggtttgaa aacgaacggg ttaaaatgat agaccagaat aaattaccct ttgaattccg
     3001 cattgcggaa tacagtaatt atctggaaat ttgtgatgct atcagagaaa tgactgttcg
     3061 cggagctcct gcaattggag ctacaggtgc ttatgccatt gctttagcag ctcaaaatgc
     3121 cccggaggaa aaattccgtg cctatttacg aactgccaga gatgaactta tagctacgcg
     3181 tcctacagcc attgatcttc gcaatggggt taattatgtt tatgaacaaa ccattaagtt
     3241 tattcctgat tataggcatt cgcgtcaggt agctatttta gcggcacggg aatttgccaa
     3301 aaaaagcatt gatgactgtt atcaaatagg caaaataggt agtgaaatag ttcctcatgg
     3361 agctcgtatt ctaactcact gtaatgcagg tgcgttagca actgtagatt ggggaactgc
     3421 tttagccgta attcggattg cccatcagca aaataaggat atatttgttt atgtggatga
     3481 aacccgtccc cgttttcaag gtgcacgctt aactgccttt gaactggaac aggaaggcat
     3541 cccgcataca ataattacag atagcgccag cggtttttat ttctggaaaa aggagattga
     3601 tttggtgata accggtgctg acagaatttg ccttaatggt gatatagcca ataaaatagg
     3661 cacttatgaa aaggcagttt tagccaaaga gcataaagta cctttctatg tagctgcacc
     3721 ttttagcact tttgatttag ccactaaaga aggagccgat attcctattg aaattcggga
     3781 tgaagatgaa attaaacgca tcaacggaac ctatcttgcc aatccaggtt caccggcatt
     3841 aaatccttct tttgatataa ctcctgctaa acttatcact ggaataatca ctcccaaagg
     3901 tatttttaag cccaaagatg cagccaggca ggtgcaagta tgatttttga catcgttttg
     3961 gaaggtggct ctataattac tatggatgac aaaaacagga ttttgtcaca atcctgtata
     4021 gctatcaaag atggtaagat acaggctatt ttccctattg gcagttcctc ttacgaaaca
     4081 aagcagaaaa tagatacgca caactgtttt attattccgg gcttaatcaa tatgcattcc
     4141 catttgccaa tgacctattt tcgcggtttg gcagatgacc tgccgttaaa tacctggttg
     4201 caaaaatata tttggcccct ggaagcaaaa ctgataaaac cgcaattggt gtatgatgca
     4261 actcttcacg gtgccagtga aatgattaaa aacggaatca gcactaccaa cgatatgtat
     4321 ttttgtatga attctatagc ggatgcctgt tcccaagtcg gtttaagggt ttttatcagt
     4381 gaagcgttga ttgataatga atcctcaaaa gaagatagag ataacactat cggaagaaga
     4441 attactcagc tgaaagagga atataaagat aatccgctta ttgattttac tttagctcct
     4501 cattctatat atgcttgtag tgtggagacc ctgaaaacct gtgctaaaat agcaaaggat
     4561 aataacatcc taattcatac tcatctttct gaaattaggg aagaagtaga gaattgcctt
     4621 aaaattcata aattgaaacc tgtggaatat ttgaaagaat taggtctttt agaggtgcgc
     4681 agtatttttg ctcatggaat ttggattgct gaagaggaaa tggaactttt agcagaaaaa
     4741 gggcttagca gtattgctat ttgcactgaa agtaacctga aactcagttc cggttttgcc
     4801 cctattaaaa agtatcagga aaaaggcatc aatctttgtc ttggaactga tggggttgcc
     4861 agtaacaata atttggattt gcttacagaa atgtctgtaa ctgccaaact gcataaagca
     4921 ctgaataatg accctacaat gcttcctgcc caagaggcat tcgcttttgt tactatcaat
     4981 gcggcaaagg cattaggcaa agaaaaagaa cttggctcac tggaaacagg taaaattgcc
     5041 gatattgctg ttgtttcttt acaggaactg gaaaatagtc ccgtttacaa tccttattct
     5101 cttttggttt atgccataaa cagtcacagc attcgcgata tgattattca gggaaaaata
     5161 gtaatgcaaa accgccaact ctgtaatgta aatgaacagc agattctgga taaggcaact
     5221 gactataagg aaatgatttt acaagcaaca gaaaaatgaa aaccatcaaa aatcgccgtg
     5281 ctgagcacga ttactttata atccaaaccc tggaagcagg tattgcccta aaaggaacgg
     5341 aaataaaatc cattcgtgcc ggaaaagtga attttaagga tagctatgcc aaaattgaag
     5401 atggagaatg ctggctacat aatttgcata tcagtccctg ggaaaaagct gcttacttta
     5461 atcacgaagc ggaacgcaaa cggaaactgc tgttgcataa acacgaaata agacgcttaa
     5521 aaaccaaagt ggaagaacaa ggtatgactc ttattccttt ggagttattc atcaatgatg
     5581 aaggactttg taaaataacc cttgctttgg ctaaagggaa aaaggcgtat gataaaagag
     5641 aagttatgcg caaaaaagac ctggaaagag accaggaagg ttgatgtcct ggtcaactaa
     5701 agaaaaatat gtgtatcgta acattagaat ttatcggaaa agatattaac ccggttaaaa
     5761 atgaacgacg aggatatcgt tcttcctaag aaaaggaaag tatatggaaa atcaagttac
     5821 cccattggaa actcacctaa accgcattac ttttttcctg aacggagaaa agattactgc
     5881 agatattcct gccggtattt ctactctgga ttggctgcat actcaaaaac agctctatgg
     5941 aacaaaatgt agttgtaacg aaggtgattg tggtgcctgc acagttgtta ttgcctaccc
     6001 ttttgaaggc ataattactt atcaggcaat aacctcctgc ctctatcctg cagccaaatt
     6061 acacggtaaa cacttaatca ccattgaggg tttaggcact ccggaaaaat tgcatactat
     6121 tcagcaagcc ctgttaacaa atcacgctat tcaatgcggt tattgcactc ccggtttggt
     6181 aatgagttta tttgctttgt ttgctatgca gccacacccc gatagagaaa tgaccttatc
     6241 tgctctggaa ggaaatcttt gccgttgtgg cacttatcca agtatattaa atgcagtgga
     6301 tgaacttgcc accaaccaaa gtgtaaaaga tatagtccct gcctggtgta gagaagtgga
     6361 aaaggattta ttccattttg cccaaaaaga ggcaatgttg gttactaaaa cagatattcc
     6421 ccaacaggtt aactgctatt taatccctac tacaactaaa cagctttttg acctttatac
     6481 cgaacatccg gaaagtgttt ttattgcagg cggaacggat attatggtgc agaaaaatat
     6541 taaccggaag gagtttccct ttttaataga ccttactcaa atccctgaac tcaaccgtct
     6601 ttatctgcag caggatggtt tgcatattgg ttctgcagta acctacaatc agctttggga
     6661 atccggaatc gtaaagaccg attttcctgt tttacataat cttatttccc aaattgcttc
     6721 ccggcagata cgtaatttgg gaactttggg aggcaatatt gccaatgctt cacctgtggg
     6781 agatactata cctcttttaa tggttttaaa tgcctctttg tggctgcaat ccaatttgga
     6841 attgaggcat ttacccttaa aagatttctt tttaggttac cacaaaacag ctctacaaaa
     6901 gggagaaatt atcaaggaaa tcattattcc tccgctaact gaagacaatt atgtaaaaac
     6961 cattaaatca gccaaacgca agagtgtgga tatttcttcc gtgattacag cagtaaatat
     7021 tgaacataaa aacggacaga ttacaaattc cgcattggca ataggtggag ttgctgcagt
     7081 accgctcctt tccagaaaat tcccaaatct attgaaaagt aaacaaatta aagctcttga
     7141 tccggctgtt attataaaag aagtggaaag tgaatttgaa cccttaaccg atattcgcgg
     7201 aacggcatta tttcgcagag aactaatccg caatcatctt attttgtatc tgcaggaact
     7261 acaggaggaa gaaaaatgaa ctcggagaca tatcatcttt ccggaacctt gcatctttgt
     7321 ggaaaatccc attttatagc tgatgaacca cctttacagg gagaacttta tgccaaattt
     7381 ctcttttctc ctgtagcgca ttcccttatc accaaattag acatctctgc cgctgccaaa
     7441 atgccgggtg tggtgaaagt tatcacttct gccgatatcc ctggagtaaa tcaaataggc
     7501 acagttatta aggatgaacc ccttttcccg gaaaaggaaa ttatgtatgt aggtcagcca
     7561 ctggcaatgg tgctggcaga agatgaatat atagtggaac ttgcagtgaa aaagattatt
     7621 ctgaaatttg aggaattacc tgccattttt gctcctgaag atgcctacag taaaaaacag
     7681 ggatatattt ccgagcgcaa aatagaaagc ggagatatag aaaatgccct aaaaaatgca
     7741 ccttacacct tgaaaggcaa gacggtaact acaggtcagg aacattttta tctggaaggt
     7801 caacgctgtt tggctgttcc ttttgagggc aatagaatca ctttgcagac agctactcaa
     7861 agcacaatgg aagttcagga agttgcttcc cgggttttag gaattccggc aaatgatgta
     7921 atcatagaag taccccgttt aggaggtgcc tttggcggaa aagaacgcgg agccacaatt
     7981 tgggcttgta tggctgcctt gggtgctttt ttaacccaaa aacccgtttt agtaaagctc
     8041 actcgggatg aggatatgca ctacacaggt aaaaggcatc ctttcacttc tctgtggcaa
     8101 gttggctatg atgaaacagg caaaatattg gcttacgatt tgttgctttt agccaacggt
     8161 ggtgcttata ccgacctttc tatagccatt ttggaaaggg ctatgtttca tagcgataat
     8221 gcttatttta tacctaatat tcgtattagg ggttatgcgt gcagaactaa tttacctcct
     8281 aatacggctt ttcgtggttt tggagctcct caaggcattt ttgtgattga aagcataatg
     8341 gataaaattg ccgctcagtt agagcttgat cctttggaaa tacgcaaaag gaatgcttat
     8401 cagaatggac aactaactcc ttacggacaa gaaataacgc agagtcagtt aaataatatt
     8461 ttcgccaaat tggcagataa agcaaactac gctttactcc ggaaagaagt ggctgaattc
     8521 aataaacata atcgtcgtta taaacgcgga attggtatta ctcctgtaaa attcggcatt
     8581 tctttcacta ctaccctgat gaatcaaggt tctgctttgg tttgggttta tatagatggt
     8641 agcgtttccg tttctacagg cggagtggaa atgggtcagg aactttctac aaaagtagca
     8701 attgtggttt cccgggtctt gggaattccg gtaaacaaaa ttagagtgga aagcagtaat
     8761 tctcaaagaa ttggaaatgc atcacctaca gctgcttcta caggaagcga tatcaatggc
     8821 aatgcagcac gccttgctgc agaaaaaatt aaagataatt tagcccattg tgcagttcgg
     8881 ctaattcagc agaaatataa ccaaacaata gacattaaag atgttgtttt caaggacaac
     8941 agggtctttg gcaaagaaga tcctgaaacc tgcataagtt ttgaagaatt ggttcgtttt
     9001 gcccattcgg aaagagttcc tttagctgct tacggttatt ataaaactcc cgggttgtgg
     9061 tttgatagag aaaagggttt ggggcatcct ttccactatt atgtttatgg agctggttta
     9121 gcggaagtgg aactggatac tttgctggga gaacacaaag ttcgcaaact gattattgtt
     9181 cacgaaaacg gaaattcctt aaatggggca attgaccgcg gacaaattga gggagctgtt
     9241 ttacaaggaa ttggctggtg cactatggaa gacctgaaat atgatgcaaa aggggtttac
     9301 ctcagcgcca atccttccac ctacaaaatt cccgctattc gcgatttacc cgaaaaattg
     9361 gttatagaaa taattccctc cctcactgaa gaagcaagtg tttttggctc taaaggaatt
     9421 ggtgagccac cttttattta tggactttct gtcttttttg ctttgcagtc cgcattcaaa
     9481 gaagcaaatc cggaaaaaga acttagcttt cctgcaactc ctgaatccct gatacaaaga
     9541 tagcgatggc agcgtaagcc cctcgggaaa tgatgaatga ttttagttac gagttctctc
     9601 ttttgacctc atagcttatt ttatcagcac cattttcctt atctgcacct tattttgcgt
     9661 tttcattatg ccgaaataaa ttcccgaagg tagtggcaaa ccttgttcat tacagccatt
     9721 gaaaacaatt ttatgttctc cggaaggaag gcaattccag acgcgaactt tttgtccttt
     9781 cacattataa atctccaaag ttcctttaga accggcagga attgtgaagc ttatcgttgt
     9841 agaaggatta aagggattgg ggtaattggt taaagtaatt ggctgaacta tttgatcatc
     9901 atttcccgta attataaatt ccagggttac aggtaatgat tcacctgcaa catattgtgc
     9961 cgtgatacaa accaaataag caacacctgc gtccaatccg gaaaagaccc agatattttc
    10021 agttgtggaa ccacaataaa caccatccag ataaatttta taatgctcaa agggagaagt
    10081 acttcctgtt aaaggttctt cccaagtaac agtaacttca ccatctataa taaaaattgg
    10141 gttttgggga ggatttaaga cagaaccgga gtattttaag ttccggaaaa atttgaaagt
    10201 gccatcataa tcgcctaaaa ccaaatcaaa atcaccgtcg tgatccaaat ccactaaagc
    10261 aggagcagca ttttggtcgg tttcaatgcc actgaataaa gttgtatttt ccacccaaag
    10321 ttgtctttgc cgtaaaaaac attgcatttc cccaaaaagg tttccagtaa ccatatccaa
    10381 atcactgtct tcatctacat ctgcaaaagt gggaacacaa tcggaaccta catctattgc
    10441 tccaaaaaag ccatttacca attcccataa aggactatag ggagtgccat tattctgata
    10501 ataaaacaaa ttacctgctt cgttaccaac cactaaatcc aggtcaccat caaaatccat
    10561 atctattaaa cgcgggcaag accatccgcc aacagaaaca gcaggcaaaa cacctgtctg
    10621 ttcaatgaac cctgttccgg tattatggta aaaataaagt cccccattca gatcacccaa
    10681 aatcacatca ggcaaaccat ctgcatctac atcgccaact gttacagcag aataaataga
    10741 gtgatgaata ttggcaaaat atgaattatc ttcctgccag gcgggagcat aagcagttcc
    10801 tgtattccga taaaatttta catagccaag ttgattaccg ctgattaagt ccaagtcacc
    10861 atcaccgtca aaatcataaa aaacaggatt actggcacct ccaacatcca aaacacctcc
    10921 aaaaagagat gtattttgtt gccaggaagg gttttctgcc gttccagtat gcacataata
    10981 ttgcaaagga cctgccgcag ttccatatac aagatcatat agtccatcac catttagatc
    11041 aactaaatca ggtgaattcc agtaagtaga cattcccaaa ccggaaaaaa aagtattatt
    11101 ttcctcccag atagggtttg tagcagtgcc aatattttga taaaaaacaa atccgtgagt
    11161 atccctaccg cataaaatat cctgtttacc atcagaatca aaatcacaaa aaaccggata
    11221 agcataaaga ccgatatcac caataagttg acagttgctt tcactaaaat tccccacagc
    11281 agcagaaccg ttgttgaaat atataattac actgccattt tcacttaaac caaccaccaa
    11341 atccagatca ccgtcattat caacatccgc tacatcagga acaggattgg aacccacatt
    11401 taaaccggag aaataacctc cgctttcagt aaaaacaggg ttagcattag ttccgctatt
    11461 caaaaacaaa tgtaaaccgg aaaaaccccc ggtcaccaaa tctaaaatgc cgtcaccatt
    11521 aatatcagcc gaaacagcaa tctctgaagc aagataagga attccagata aaaggtcagg
    11581 tcctggcgca aaaacaggat ttgtagcagt tccattattt tgcaggtaaa gtggaggacg
    11641 gtttgtattt cccagccaaa aatcaaaatc accgtcacta tccaaatcca taaatcgggg
    11701 ttgggaaaaa gaaaaactgg gaactccact gggattgaaa acactgttat ccatttccca
    11761 gggctgagca ttcaggcata aagccagaaa aaggataacg ataaatatat aagtcctttt
    11821 cattttttac ctcctgaaga gagtatataa ctattaattt ttatccctgt tttactgtca
    11881 atcatttgct taatcttgaa ttgcggatat ataaagtaga taaggaaaat aggtctgact
    11941 gaaaaaggta aaaatgcttt taaggtctca tttgtttatc gtctacaaaa taaggaaatg
    12001 attatgatta aacgatttat tgcttctcgc atattaagta atctgcataa aggaaaacca
    12061 gttttaattt tgggagcaag gcagagtgga aagacaactt taataaattt aaataatgtc
    12121 atctgggaaa cggggtttgt aaattctttc tttgccgaag aaagagaagg tttctaaagg
    12181 gatttatgtt ttcagtattt accgaatcgg aattgggtaa cccaaaagct aaatatgtct
    12241 atcatccatt tccactgctc aatatgaagt tatgtgttgt ccttcttgtt ttaatgggca
    12301 atttatagaa caaaattact actttctctc tttgtaaaac tattctcttt tcctctttct
    12361 ctccttgtaa aactaacctc ttttcctcct tgtaaaacta ttctcttttc ctctttttct
    12421 ccttgtaaaa ctattctctt ttcctcttta tctctttgta aaactattct cttttcctct
    12481 ttatctcttt gtaaaaccaa tctcttttcc tctttatctc tttgttaaaa aaaccctcgg
    12541 tgctctcggt gtcctcagtg tttttggtgt aaaaaagcac ctacataaac agggaacaat
    12601 ctttttcctt gactttgtca cattccgctt tttttatggt ttttgtaaaa tattaaggaa
    12661 aaggatatgg ttggaattgt tctttcgtac actactctcg gaatagatgc ctatcagatt
    12721 tcggtagaag cggatattgg aaccggtttt aatccgatga acattgtagg gatggcttcc
    12781 aatgctgtta aagaaagcaa ggatagagtt attgctgcca tcaagaattc cggttatcaa
    12841 ataagtactc aacattatac aataaacctt gctccggcgg atttgaaaaa ggatagcgca
    12901 gctttggatt tgccaattgc tttagccatt ttgcaatgca acgagatttt tagaattatt
    12961 gactacgata caattgcgat gattggtgag ctctctttag atggatttgt tcgtcctgta
    13021 gcaggtgttt tgccaattgc tctggcagct aaaaaagacg gtataaatac tcttattgtt
    13081 cctctggaaa atgccgaaga agcagccatt attgaaggtt tgaatgttat tcctgttacc
    13141 tctttaagag aatgtgtcag ttggctttgt aaagaaacag agattatggc tgctaatgta
    13201 gaccgggaga agatttttca ggtattgaac gattttcccg tggatatgag tgatgtgaaa
    13261 ggtcaatttc aagtgaaacg cgctttggaa gttgctgcag caggaggtca taatgttttg
    13321 atgattggtc ctccgggaag tggaaaaaca atgttagccc gcagagttcc gacaattttg
    13381 ccggaattga atctggaaga agcattggaa gcaactaaaa ttcattccgt agccggctat
    13441 tccaaaaatt ttcgcaatgg tattttaacc accagacctt ttcgctcgcc tcatcatacg
    13501 attagtgatg
//
LOCUS       DAOPFL010000039        13467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5353_length_13467_cov_84.511259, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000039 DAOPFL010000000
VERSION     DAOPFL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13467
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5353_length_13467_cov_84.511259"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..1167)
                     /locus_tag="PLG69_04225"
     CDS             complement(322..1167)
                     /locus_tag="PLG69_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPI42750.1"
                     /translation="MFITVSVPKNEQLNSYAPGSCEREKLKKALSELHNDFIFAHTSA
                     DVKSLAVACLRGAFEFQGQKCSAASRLYIPDTIYDEWFKEMVEMLETVKMGDVMDFSN
                     FINAVIDKESFDRIKNYIDYAKNSPVAEIIWGGNCDDSVGYFIQPTLIKTTDPMFCTM
                     QEEIFGPVLTVYVYPEKDFSKTLDLCDKTSPYGLTGSIFAQDRKAILEANNVLRHSAG
                     NFYINDKPTGAVVGQQPFGGGRSSGTNDKAGSALNLQRWISARSIKENFVPDIEYKYP
                     FMAEA"
     regulatory      complement(1247..1365)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(1520..2617)
                     /locus_tag="PLG69_04230"
     CDS             complement(1520..2617)
                     /locus_tag="PLG69_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42751.1"
                     /translation="MKKILTFISTLLLISIISAINFDYDADMLTRAAAYNDYYKKDGG
                     HIDSRLRLGLNSELTEGLNFRLAIEIGEITWGDNATGGNIGTGGINIETSELYLDYYM
                     PAIKSNIKAGLQYLADHRSLVLDHYGAGVILYTDIEGMKAQAGMLKNLENNRFAHDDY
                     TIFLASLEATSPVVWGFNSYIGYNDRNDDGEISFLPYVTLTAGPATLDLNPFVCYQFI
                     DGPDKTGFGAAVKAEIDVTPIQVGADILIASEDGLVPYCSYYQNGLFIYGYNIIHDGL
                     NLYWTDELYHNNEETFISAVGSIRFPVTEKHTLFAAGGWLKDLGMELNGGIESQLVKD
                     MLKLYLYGAWGKNDDSDVNNYATGVSLQLNF"
     gene            complement(3177..4235)
                     /gene="arsB"
                     /locus_tag="PLG69_04235"
     CDS             complement(3177..4235)
                     /gene="arsB"
                     /locus_tag="PLG69_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPI42752.1"
                     /translation="MQQTTEISKGISFFEKYLTFWVIICMAVGVLIGKYIPEIPYFLR
                     KFEFAQVSIPIAVLIWLMIYPMMMKVDFASIKNVGKNPQGLIVTWIVNWLIKPFTMYA
                     IAWFFFYIVFKSVIPTNLAKDYLAGAILLGAAPCTAMVFVWSHLTNGKPAYTVVQVAT
                     NDLIILVAFTPIVALLMGISGIKIPWDTLILSVILFVVIPLAGGATTRYSVIKHRGED
                     YFLKTFIPKFNNVTISGLLLTLVIIFAFQGEVIINNPLHIILIAIPLTIQTYLIFFIS
                     YIFTKQIKLPHNIAAPAALVGASNFFELAVAVAISLFGADSPVALATTVGVLVEVPVM
                     LNLVSIANKTKQWFIPAN"
     gene            complement(4342..5433)
                     /locus_tag="PLG69_04240"
     CDS             complement(4342..5433)
                     /locus_tag="PLG69_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPI42753.1"
                     /translation="MKYLPVDVLQSFMQEVFIKLGVPEEDAQICSDVLIASDLKGIES
                     HGIGRLKMYYDRIKAGIQNPVTNIDVLRDKCATAVWDGNNGMGQVISKKAMQAAIDKA
                     EKYGLGAVAVRNSTHFGICGYYAEMAIRQNMIGLVFSNARPSICPTNGVSPILGTNPI
                     CFGAPTDLPYPFLYDAATSISQRGKVEQLAREEKETPLGWAIDLEGKPYTDTKGLLID
                     LLKQKAAMLPLGGTEEITGSHKGYGLATAVEILCASLQKGNYLNGLMGEDENGKPAPY
                     RLGHFFMAINIEFFTDLVDFKKITTSICKDLQNSQLFPGKNRIYVAGEKEYEIEQKVR
                     KQGIPIIPNLEKNIRIIQDELGLNLLNLD"
     gene            complement(5447..6628)
                     /locus_tag="PLG69_04245"
     CDS             complement(5447..6628)
                     /locus_tag="PLG69_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPI42754.1"
                     /translation="MYREPYSFNISRFMDEERFEKIMAFAEKVPTPCLIVDLDIVRNK
                     YLELQASMPNYKIYYAVKANPMLEVIMLLNELGANFDLASPYELDQMLNLGIEPSRLS
                     FGNTIKKVNDIKYFYEKGVRLFATDSKLDLANLAKYAPGSKVLFRILTEGTGADWPLS
                     RKFGSHPDTIYHLIVKAAKMNLQPWGISFHVGSQQRDIGQWDDAVARCKYLFDAVAEE
                     GIELKMINIGGGFPANYVDPTFSIQEYATEIQRFITEDFGDNLPEIILEPGRSLVADA
                     GVIVSEVINVARKSKNNLYQWVYLDVGKFGGLIETIDESIKFPIYFPRKGLAEEIILA
                     GPTCDSMDILYEHYRYRMPEGTVPGDRVYIFTTGAYTQSYSSVNFNGFPPLKAVIFEE
                     K"
     gene            6757..7905
                     /gene="mnmA"
                     /locus_tag="PLG69_04250"
     CDS             6757..7905
                     /gene="mnmA"
                     /locus_tag="PLG69_04250"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPI42755.1"
                     /translation="MQKKTSVAVGISGGIDSAMTAYLLQESGYRVIGITMQTYDENIE
                     MQKTVKSGCFGPAQEENILSASLLCRKLGIEHYIIPLQEEFRHKVIDYFCETYIKGKT
                     PNPCLVCNAYIKFALLPKKAHSLGLEFDYFATGHYAKIGFNNTLKRYQIRKAKDANKD
                     QSYFLAFLSQEQLAQTLFPLGDYTKQEIKIMAIEKGFTELVERKESQDFLQSSDLEVL
                     FKPEDFNPGEIIDMQGKVIGKHKGIINYTIGQRRNLGISGMPEPYYVLQIDAENNRIV
                     LGPKEYLFKKKCSATDMNWVSLPAPEKKFRAKAKIRQQHSPADCIVTPINDNTIEVVF
                     KEPQLSITPGQGLVLYLDDLLLGGGFIAFSNNEDVETKDLEGFRRGES"
     gene            complement(8382..10115)
                     /locus_tag="PLG69_04255"
     CDS             complement(8382..10115)
                     /locus_tag="PLG69_04255"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPI42756.1"
                     /translation="MASKSSVEYYSNIKEMSPIRAIAETLMNNHLVRKVDIREAYELA
                     KNQPGVSVTDLPMYPEFVKLHNLPADAKVINDCHGNIVGRTAKARRFYHRLSPSLKNK
                     LESDFREAVWQMQHYPLIKAEAVLGMDKDLMLKATFVTTESDAANLFFWLLNFAPYES
                     LKEEYEASPKLPIQDIILVAFNEWTCDDDFYNNVGAPQLALVDEKHNVMVNLGMRYFG
                     ERKKGTLTMAWTSGIRIGMAACHGGIKEIDFSTCVNPEYHKLGKRSIAFYGLSGTGKS
                     SHTNNADNAGTLPEGFSKVVLHDDAFQIDLENKLCRVWEPTLFDKTDSRTVDHPDWKY
                     TLALMNHTVLDVNGKLIPYGQDIRNSNGRALFDRSLLGKYVNRCAFPKALVWLMKDSI
                     LPPALKLEDKYLAIAMGAALMTQRNRAENVAEEELNKLVFEPFANPFRVYELYRDVEA
                     FLKVADTGADFYCFNSRGYWRESDDILEPIPLNTSLTLQTAILMDQLQWEPWDLLPGS
                     MIPTRESVEAILPGYYDRYHSNLRINYDKYIAVMKDRFQQRKDFLKSSDLTERPEIQE
                     KLIQSLQVNAD"
     gene            complement(10180..11358)
                     /locus_tag="PLG69_04260"
     CDS             complement(10180..11358)
                     /locus_tag="PLG69_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPI42757.1"
                     /translation="MSLLDKCYKFTDARKVMAMGYYPYFREITSEQDTEVMCNGKKML
                     MLGSNSYLGLTNHPKVKEAAIKAIQKYGSGCAGSRFLNGTLDIHLELENELAKLVGKE
                     EAICYPTGYQANLGCISTMVGKNEYIITDKYDHASIIDGAILAEGKMLRYKHNDMASL
                     ERILKEIKGRNSLIVVDGIFSMEGDIANLPVIIELAEKYNAEVMVDEAHSLGVLGNKG
                     AGTTAHFGLTDKTAFIMGTFSKSLASVGGFIAADEAIIHYLKHKSRALIFSASLPPAS
                     TASVLAALKIMQEEPERIAKLWENTNYMLKEFQAMGYNTGASCTPVVPLHIGNMMTTF
                     NMWRRLGEEGVFINPVVPPAVPPSSCLIRTSFMATHTKDQLDMALEKFRMIGKELNII
                     "
     gene            complement(11370..12431)
                     /locus_tag="PLG69_04265"
     CDS             complement(11370..12431)
                     /locus_tag="PLG69_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPI42758.1"
                     /translation="MNWTKWIFYSLLMALIVLGLFFYHPIIYYLIYAFIFSYILDPFI
                     SWFERKRFPRWLAVLTIYIIIAGIIALFTSRLVPALVQQGNNLFNILSSGEQLTGQYI
                     ITLPFIRRIYEFLQELDAQIPGLGMAPKLIEILDQANVFLAKIPKLLIDNYKKIFSTI
                     SIIFTVPLISFFLLKDKYKLRKGIVKMFSNRYFELCIIMMRKIDSTIGNFLRAILFEI
                     IAVGILASTALTIVGVPYSILIGATAGVANIIPYFGPFLGGALAVVTVLFNGGTILSA
                     ITVILAMYIVQLIDNNIVYPVVVGGTINMHPLLVLLTLIAGGWYGGILWMLISVPLVY
                     LTYNLIKVLYTNLKGFKII"
     gene            complement(12428..12913)
                     /locus_tag="PLG69_04270"
     CDS             complement(12428..12913)
                     /locus_tag="PLG69_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPI42759.1"
                     /translation="MLKDVFDWLKTIWKKPDKYADLKKFPVLSELSSFELYLLNNFLH
                     KRNFNSGELLYDKDHPLEVIYFIFSGEVEVTGPTYPKGRYVVRELQILGLIDMFKEET
                     RSSSATALTEVTAYAVSRYDLSDLFRQNPNLGVKMLTAFCQTLSNYIFNISSPESAKE
                     L"
     gene            complement(12917..>13467)
                     /locus_tag="PLG69_04275"
     CDS             complement(12917..>13467)
                     /locus_tag="PLG69_04275"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPI42760.1"
                     /translation="HYVLPEYRDYAYQNRPLPIKEGQTISQPLMIAIMLQLLDLQPTD
                     IVLEIGTGSGYQSALLAEIVKEVCTVERLETLSLNAQKILRNAGYNNIYFRIGDGYSG
                     WQKAYPPHKEFDKILVSAAAREVPKHLVEQLKMGGKMAIPIGKTGFQILYIVTKTEDG
                     LDIAEAGGCSFVPLVCDDKTLR"
BASE COUNT         4398 a         2674 c         2304 g         4091 t
ORIGIN      
        1 aatcccaata ataccgatga attaaaatgc ataaacattt tttctgtctt atatggattc
       61 ctgctttcat aggaatgaca ctggactttt atttcaagat ctgatataaa atatttttag
      121 ataatttcat tatctatggc aaattggaat tattcacctc atttttcctc aactgcattg
      181 ttcaggtcta tgcattatcc gtataaaatt gtttaatgga ttgattatcc tgaataatca
      241 ggattccttt ttattaaaaa aataaagcag ggtggcaatt gagcaattgc cactctgcac
      301 attagtaagt gataatttta attatgcctc agccataaaa gggtatttgt attcaatatc
      361 cggaacaaag ttttctttaa tagacctggc tgaaatccat ctttgtaaat ttaacgctga
      421 accggcttta tcatttgtac cggaagacct tccacctcca aaaggttgct gtccaactac
      481 agcgcctgtt ggtttatcgt taatgtaaaa atttccggca ctatgccgta gaacattgtt
      541 tgcttccaga atagcttttc tatcctgagc aaaaatagaa cccgttaaac cataaggaga
      601 ggtcttatca caaagatcaa gagttttgct gaaatccttt tccggatata cataaacagt
      661 taaaaccgga ccaaagattt cctcttgcat agtgcaaaac atcgggtctg tggttttaat
      721 gagagtgggt tgaatgaagt agccaacgga atcgtcacaa tttccacccc atataatttc
      781 tgccacaggt gaatttttgg cgtaatcaat gtagttcttg attctatcaa aactttcttt
      841 atctataaca gcattgatga agttggaaaa atccataaca tcacccattt taactgtttc
      901 cagcatttcc accatttctt tgaaccattc atcgtaaata gtatccggta tatataaacg
      961 ggatgcggca ctgcattttt gaccctgaaa ttcaaaagca cctcttaaac aggcaactgc
     1021 taaggactta acatctgccg aagtatgagc aaaaataaaa tcgttgtgca gttcactaag
     1081 tgcttttttt agcttttctc tttcacaaga gccgggagca taactattca gttgctcatt
     1141 tttaggaacg gaaacggtaa taaacatttc tatctccttt tagtttattt cgttattttt
     1201 agaatgaaaa ggagaaatag acatagatgg accaggcaca cctatcctgt ggctcaattc
     1261 ttcacctttt cacattggaa ggcaaatccg ttttcagata aaccctatcg gctaacatcc
     1321 ctgccggaga agtttaggac tgttagctcg gtgacaaaac aaccttttaa ttatgcaata
     1381 ttatatcaag ttatgtaaaa tagtttactc tataaatcgc tatagttgat atacataact
     1441 ggttgaaatt attgttaatg aaaaaagccc ggctgtgcaa aaccgggctt ctaatagggg
     1501 atttttttaa gtagtttaat taaaaattaa gctgcaagga aactccggta gcatagttat
     1561 tcacatcact atcgtcattt ttgccccagg caccgtaaag ataaagtttc aacatatctt
     1621 taaccagctg ggattctatg cctccattca gttccattcc aaggtctttt agccagccac
     1681 ctgcagcaaa taaagtatgt ttttccgtta ccggaaaacg aatagagcca acagcactga
     1741 taaatgtctc ctcattattg tgatataact cgtcagtcca ataaagattc agtccatcgt
     1801 gaatgatatt atagccataa atgaagagtc cgttttggta atatgaacag taaggaacca
     1861 aaccatcctc ggaggcaatg agaatatcag ctccaacttg aataggagtt acatcaattt
     1921 ctgcttttac agcagcgcca aaacctgttt tatcaggacc atcaataaac tgataacata
     1981 caaacggatt caaatctaaa gtagcaggac ctgcagtaag agttacataa ggcaggaaag
     2041 atatttcacc gtcatcgttt ctatcattat agccaatata actattaaat ccccaaacca
     2101 caggagaagt tgcttccagg gatgcaagga agattgtata atcatcgtga gcaaaacggt
     2161 tattttccag gtttttcagc attcctgctt gagctttcat tccctcaata tccgtataaa
     2221 gaataactcc cgcaccataa tggtctaaaa ccaaactacg atgatccgct aaatattgta
     2281 aaccggcttt gatgttgctc tttatggctg gcatataata atccagataa agttcactgg
     2341 tttcaatatt tatgccacct gttccgatgt tacctccggt agcattatca ccccaggtaa
     2401 tttctccaat ttcaatagcc aacctgaaat ttaacccttc cgtcaattct gaattgagac
     2461 ccaacctcaa acgactgtct atatgaccac catccttttt gtaataatca ttataggcag
     2521 cagcacgcgt aagcatatcg gcatcataat caaaattaat ggcacttata atgcttatta
     2581 acaatagggt tgagatgaag gtaagaattt ttttcacttt tctctccttt ttttatttgt
     2641 ctttaataat aagataattc attaacctgc caatgtcaag tgaatagcaa aagaaacgca
     2701 ttcgtctaat ccgaaaataa aagcattgag ttggcattat aagagcatta aaaattattt
     2761 cctgctggaa tattgtagca gttcaaatca atataaacac atccaagaag ctttaaaaat
     2821 ttctcgttcc attgtagtaa taatagcata tacttaaatt tcggatgtct attgccgagc
     2881 tatttatgtt gcaagttgta aaggcaattc gaatcatcta actatatcta aatactcccg
     2941 tcggactaac tatcactcag ttgaatgctt ccactcttca gccatcctgg aatgctgttg
     3001 cgcaagcaaa ttcatacaaa gttttggctg ctgaggatcc taattctgca gaacggacaa
     3061 taattctgga aaatatagct gcaacccagc taaatattca aattttaccg gaacagccat
     3121 ttcgtttcta caaagtggtt gcagttagat aataatatcg gaagataatc taaactctaa
     3181 tttgcaggga taaaccactg cttggtttta ttagctatac ttaccagatt aagcataacg
     3241 ggaacttcaa ctaaaacacc tacagttgta gccaaggcaa caggtgaatc cgctccgaaa
     3301 agagatatag ctactgccac tgcaagttca aagaaattgg atgctccaac taaagctgct
     3361 ggtgcagcaa tattatgggg taattttatt tgtttggtga atatatagct tataaagaag
     3421 attaaatagg tctgaatcgt cagtggaatg gctatgagga taatatgaag aggattattg
     3481 ataataactt ctccctgaaa agcaaagata ataacaaggg taagcaacaa accgctaata
     3541 gtaacattat tgaatttggg aataaaggtt ttcaggaaat aatcctctcc cctatgtttt
     3601 atcactgaat atcgcgttgt tgctcctcca gctaaaggaa taacaacgaa taagataacg
     3661 gagagaatta atgtatccca agggattttt attccactaa tgcccataag taatgctaca
     3721 ataggtgtga aggcgaccag aattatcaaa tcgtttgtag ctacctgaac taccgtataa
     3781 gcaggtttac cattagttaa atggctccag acaaaaacca tcgctgtgca aggtgcagca
     3841 cccaacaaaa tggctccagc aagataatct tttgctaaat ttgtaggtat aacagattta
     3901 aatacaatat aaaagaaaaa ccaggcaata gcatacattg tgaagggttt aattaaccaa
     3961 tttacaatcc aggtaacaat taatccctga ggatttttac ctacattttt aatgctggca
     4021 aaatcaacct tcatcatcat cggatagatc attaaccaaa taagaacggc aatcggaatt
     4081 gaaacctgag caaattcaaa ttttcgcaga aaataaggta tttcaggaat atattttcct
     4141 attagaacac caactgccat acatataatc acccaaaagg ttagatactt ttcaaaaaaa
     4201 ctgattcctt tacttatttc tgttgtctgt tgcatcttat ccaccttttt gttgttatat
     4261 tttttaaggg gagattttaa attcattaat aattctttaa agaatattag tattgtgcat
     4321 agaatcctgg caaaatgtgt atcaatctaa attcagtaaa tttaagccca gttcatcctg
     4381 tattattcta atattctttt ccaggttagg aataatagga atgccttgtt tacgcacttt
     4441 ttgctctatt tcgtattctt tttcaccggc tacatagatt ctgttttttc cggggaaaag
     4501 ttgggagttt tgtaagtcct tgcaaataga agttgttatt tttttaaagt ccaccaaatc
     4561 cgtgaaaaat tcaatattaa tagccataaa gaagtgtccc aaacggtagg gagctggctt
     4621 tccgttttca tcttctccca ttaaaccgtt caagtaattt cctttttgta aggaagcgca
     4681 gagaatttct acagcagttg ccagtccata acctttatga cttccggtaa tttcttctgt
     4741 tccacctaag ggtaacattg cagctttttg ttttagcaaa tcaatcagca gaccttttgt
     4801 atccgtatag ggttttcctt ccaaatcaat agcccaaccg agaggagttt ctttttcctc
     4861 gcgagccagt tgttctacct tacctctttg ggaaatagaa gttgcagcat cgtataaaaa
     4921 aggatagggt aaatcggtag gcgctccaaa acatataggg ttagtcccta aaataggaga
     4981 aacaccattt gtagggcaaa tggaaggtcg ggcattagaa aaaacaagtc cgatcatatt
     5041 ttgccgaata gccatttctg cataataacc gcaaattcca aaatgcgtgg aattacgaac
     5101 ggcaacagca cccaaaccat atttttcagc tttgtcaatt gctgcttgca ttgctttttt
     5161 acttattact tgtcccattc cattatttcc atcccagacg gcagtagcgc atttatcgcg
     5221 gaggacatca atgttagtta cgggattttg aattccggct tttatacgat cataatacat
     5281 ttttaggcgg ccgattccgt gagattcaat accttttaaa tcactggcta ttaacacatc
     5341 gctgcatatt tgtgcatctt cttctggcac tccaagttta ataaatacct cttgcataaa
     5401 actctgcaga acatcaacag gcaaatattt catttctaaa tactccttat ttttcttcaa
     5461 aaataacagc tttcaaggga ggaaaaccat taaaattaac gctggaatag ctttgcgtgt
     5521 aagcaccggt agtaaaaatg taaactctat ctccgggaac ggttccttcg ggcatccgat
     5581 aacgataatg ctcatagaga atatccatac tgtcacaagt aggaccagct aatataatct
     5641 cttccgccaa acctttacgc ggaaaataga tggggaattt tatggattca tcaatggtct
     5701 caattaaacc gccaaatttt cctacatcta aatacaccca ttgataaaga ttatttttgg
     5761 atttacgagc tacattaatt acttcggata caatcacacc agcatctgca accagagaac
     5821 gacccggttc caaaataatt tccggaaggt tatctccaaa atcctctgta ataaaacgct
     5881 gaatttccgt agcatattcc tgaatagaaa aagtgggatc aacatagttg gcaggaaaac
     5941 cacctccgat attaatcatt ttcagttcaa ttccttcttc ggcaacagca tcaaaaagat
     6001 atttacaacg agctacagca tcgtcccatt gccctatatc tcgttgttga gagcccacat
     6061 gaaaagaaat tccccagggc tgtaaattca tctttgccgc tttcacaatt aaatgatata
     6121 tagtatcagg atgtgaacca aattttctgg ataggggcca atcagcgcca gttccttccg
     6181 ttaaaatccg aaataagacc tttgaaccag gagcatattt tgccaggtta gcaagatcaa
     6241 gcttgctgtc agtagcaaaa agacggacac ctttttcgta aaagtatttg atatcgttaa
     6301 cttttttaat ggtattgcca aaacttaaac gggagggctc aattcctaaa ttcaacatct
     6361 gatcaagttc ataaggagaa gcaagatcaa aattggcacc aagttcattc aataacataa
     6421 taacttccag cataggattt gccttaactg catagtaaat tttataatta ggcatacttg
     6481 cctgcagttc caaatactta ttacgaacta tatccaagtc cacaatcaaa caaggagttg
     6541 ggactttttc cgcaaatgcc ataatttttt caaaccgttc ttcgtccata aaacggctga
     6601 tattaaagga atatggttct ctatacaatg ttttgtcctc tttggtatta atcagttttt
     6661 cccaaaattc acaaagccat ttaatgtcaa ataaaaagta tttgacacat atcccgcttg
     6721 cattgaggtg aaatatatta aaataaggta actgaaatgc agaaaaaaac aagtgtagca
     6781 gtaggaatca gcggagggat tgactccgca atgactgctt atttacttca agaaagcggt
     6841 tatagagtaa ttggcatcac tatgcaaact tatgatgaaa acatagaaat gcaaaaaacg
     6901 gtaaaaagtg gctgttttgg tcccgcgcag gaagaaaata ttttatcagc ttctctgctt
     6961 tgccgaaaat tgggaattga gcattatatt attcctttgc aggaagaatt tcggcataag
     7021 gtaattgatt atttttgtga gacatacata aaaggaaaga ccccaaatcc ctgtttggtc
     7081 tgtaatgcct atatcaaatt tgccttgtta cctaaaaaag cgcattcatt gggactggaa
     7141 tttgattatt ttgctacagg tcattatgcc aaaatcgggt ttaataatac ccttaaacgc
     7201 tatcagataa gaaaagcaaa ggatgcaaac aaagatcaat cctatttttt ggcttttcta
     7261 tctcaggaac aactggcgca aacccttttt ccattagggg attatactaa gcaggaaata
     7321 aaaataatgg caatagaaaa gggctttact gaacttgttg agcgcaagga aagccaggat
     7381 tttttgcaaa gttcagatct ggaagtcctt ttcaaacctg aggactttaa tccgggagaa
     7441 attattgata tgcaaggtaa agttatcgga aagcacaagg gaattataaa ttacactatc
     7501 ggtcagagac gaaatttggg aataagcggt atgcctgaac cctattatgt tttgcaaatt
     7561 gatgcggaga ataacagaat tgttttgggt cctaaggaat atttgtttaa aaagaaatgt
     7621 tcagcaacgg atatgaactg ggtttctctt cctgcacccg aaaaaaaatt tagggcaaaa
     7681 gccaaaatac gacagcaaca ttctccagct gattgtattg taacccctat aaatgataac
     7741 actatagaag tagtttttaa ggaaccgcaa ctttccatta ctccgggaca ggggctggtt
     7801 ttatatttgg atgatttact tttgggaggt ggatttatag cattttcaaa taatgaggat
     7861 gtagagacaa aagatttaga gggttttaga aggggagaaa gctaaaattt ttgatagctc
     7921 tatagatcat acaataagag cgatgtgcgt gtaaacccct gaataaaaga acttgataat
     7981 ggtactaact aattataatt aaagtagata cagccgtcat tagtcctttg tgctataaaa
     8041 atattttccg aagtataatt catttgagca caaattactc ctttcttaca ttgtaaacga
     8101 tttcctactt cttgcttaat tagttatggt agagatgcgg tggagccggt cgggaaaaaa
     8161 ccaataattg gcaccataac tccactacaa ctaattagag aaggataaaa aaagaatagc
     8221 gggaaaatta tcccgctatc tattaaacat tcttagggca agttactttt tactatataa
     8281 ttaaaaggaa ttcaaaagaa ggctacattc agcatttgcc caatgggaat ttggcaattc
     8341 ttttttatta tgcttataga gaaatatgcc agtgtataaa tttaatcagc atttacctgt
     8401 aatgactgta tcaatttttc ctgaatttcc ggtctttcag ttaaatcact gctctttaaa
     8461 aagtcctttc tttgctggaa tctgtctttc attacggcaa tatatttatc gtagttaata
     8521 cgcaggttgg aatgatatct gtcgtaatag ccgggtaaaa ttgcctcaac agattctctt
     8581 gtaggaatca tagagccagg taataaatcc cagggttccc attgcagctg gtccattaaa
     8641 atagcggttt gcagagttaa cgaagtattt agaggtatcg gttccaggat atcgtctgat
     8701 tccctccaat agccacgcga attaaagcag taaaaatccg ctcctgtatc agccactttt
     8761 aaaaatgctt ccacatcacg atagagttca tacacccgaa atggattggc aaagggctca
     8821 aaaacgagct tgttaagttc ttcttcggca acattttccg cacgattacg ctgagtcatt
     8881 aaagcggctc ccatagctat tgccaaatat ttatcctcca attttaaggc aggtggcaaa
     8941 atagaatcct tcatcaacca aaccaatgct ttgggaaaag cacagcggtt cacatatttt
     9001 cccaaaagag aacggtcaaa aagggctctg ccattggaat tacgaatatc ctgtccgtaa
     9061 ggaataagtt tgccattaac atccaaaacc gtatgattca tcaaagccag agtatatttc
     9121 cagtcgggat gatcaacggt tcggctatcc gttttgtcaa ataaagtcgg ttcccaaacg
     9181 cgacacagct tattttccaa atcaatctgg aaagcatcat catgtaaaac aaccttggaa
     9241 aagccctcag gcaaggttcc tgcattatcc gcattattgg tatggctgga ttttcctgtt
     9301 ccggaaagtc cataaaaagc gatagacctt ttacccaatt tatgatattc aggatttaca
     9361 caagtgctga aatcaatttc tttaattcct ccatgacaag cagccatccc gattctgatg
     9421 ccggaagtcc aagccatagt taaagttccc tttttccgtt caccaaaata acgcattccg
     9481 agattaacca taacattgtg tttttcatcc accaaagcca gttgtggtgc ccctacattg
     9541 ttgtaaaaat cgtcatcgca agtccattca ttgaaagcaa ccaaaataat atcctgaata
     9601 ggaagcttgg gacttgcctc atattcctct ttcaacgatt cataaggggc aaaattcaac
     9661 agccaaaaga aaagattggc agcatcactt tcagtagtta caaaagtagc tttcagcatc
     9721 aggtctttat ccatccccaa aactgcttcg gctttaatta atggataatg ttgcatttgc
     9781 cacactgctt cccgaaaatc actttccagc ttatttttca gggaaggact taacctatgg
     9841 taaaatctgc gggctttagc ggttctgcca acaatgttac catgacaatc gttgataact
     9901 tttgcatctg caggcagatt atgcagcttt acaaactccg gatacatagg caaatcggta
     9961 acacttacgc cgggttgatt ttttgccagt tcgtatgcct cgcgaatatc cactttccga
    10021 actaaatggt tattcatcag ggtttcggca atagctctga taggagacat ttctttaatg
    10081 ttggaataat attccacact gctcttgctt gccatttttt tcctccatat atggcttatt
    10141 ttatatgttc aaaaaagtta agatgatacc tattataaat taaatgatat ttagttcctt
    10201 gccaatcatt ctgaattttt ccagagccat atcaagctga tctttggtat gtgttgccat
    10261 aaaagatgtc ctaattaaac aggaactggg aggaacagca ggaggaacta caggatttat
    10321 aaaaacacct tcttctccaa gccgacgcca catattaaaa gtagtcatca tattccctat
    10381 atgtaacgga acaacaggag tgcaactggc tccggtatta taacccattg cctgaaattc
    10441 tttcagcata taattcgtat tttcccaaag tttggcaatt ctttcgggct cttcctgcat
    10501 aattttaagc gctgctaaaa cgctggcagt tgatgcagga ggaagcgatg ccgaaaaaat
    10561 aagagcccgg gatttatgtt ttaagtaatg aataattgct tcatcggcag caataaaacc
    10621 acctacagat gccagggact tgctgaatgt gcccataata aatgctgttt tatcagttaa
    10681 accaaaatga gcagtagttc ctgctccctt atttcccaaa acacctaaag aatgtgcttc
    10741 atcaaccata acttcggcat tatatttttc cgccagttca attattaccg gaagattggc
    10801 tatatcccct tccatagaaa agataccatc tacaacaatt aaagaattcc tgcctttaat
    10861 ctccttaaga atgcgttcca aagaagccat atcgttatgt ttgtaacgca gcattttccc
    10921 ttcggcaaga atagcgccat ctattattga ggcatgatcg tatttatcag taataatata
    10981 ttcattttta cctaccatag tagaaataca ccctaaattt gcctggtaac ctgttggata
    11041 acaaattgct tcttctttac cgaccaattt tgccagttcg ttttccagtt ccagatgaat
    11101 atctaaggtt ccatttaaaa aacgactccc tgcacagcca ctaccatatt tttgaatagc
    11161 ttttatggct gcttctttca ctttgggatg attagttaag cccaaataac tattagaacc
    11221 caacatcaac atttttttac cattacacat aacttccgta tcttgctcgg aagtaatttc
    11281 ccgaaaatag ggataataac ccatagccat cactttacgt gcgtctgtaa acttgtaaca
    11341 tttatccagt aaactcacac tttcctcttt tatatgattt tgaaaccttt caggttagta
    11401 tacaaaactt taatcagatt atatgttaaa tacacaagag gcaccgaaat aagcatccag
    11461 agaatgcctc cataccagcc cccggcaatt aaagtaagaa gaactaacaa aggatgcata
    11521 ttaatagttc cgcctacaac aacagggtaa acaatattat tatcaatcaa ctgcacgata
    11581 tacatcgcca gaattacggt gattgcagaa agaatagtac caccattaaa cagaactgtt
    11641 actacagcta aagcaccccc taaaaaaggt ccaaaataag gtataatatt tgccactcct
    11701 gccgtagcac caattaaaat ggaatatggc actcccacaa tcgttaaggc agtagaagct
    11761 aaaatgccaa ctgctattat ttcaaacaag atagctcgca aaaagttacc tatggtgctg
    11821 tctattttcc gcatcataat gatacagagt tcaaaataac ggttgctaaa catttttaca
    11881 atgcccttcc tcaatttgta tttatccttc aagagaaaga aagaaatcag gggaaccgtg
    11941 aaaataatag agattgtgga aaatatcttt ttatagttat ctatcagtaa cttaggaatt
    12001 ttagccaaaa agacattggc ttggtctaat atttctatca gcttaggtgc cattcccaaa
    12061 ccgggaattt gagcatccaa ttcctgtaaa aattcgtata tccttctgat aaaaggtaaa
    12121 gttataatat attgcccggt tagctgttcc ccactactta aaatattaaa aaggttgttc
    12181 ccttgttgaa ctaaagccgg aaccaaacga gaagtaaaca aagcaataat tcccgcaatt
    12241 attatataaa ttgtcagcac tgcaagccag cggggaaacc tttttctctc aaaccaactg
    12301 ataaaaggat caagaatata ggaaaaaata aaggcataaa ttaaatagta aattatggga
    12361 tggtaaaaaa acaatcccag cacaatcaga gccattaata aactataaaa aatccacttc
    12421 gtccagttca tagttctttt gcgctttccg gagatgaaat attaaaaata taattgctca
    12481 atgtttggca gaatgcagtt aacattttaa cacctaaatt aggattctgc cggaacaaat
    12541 cactcaagtc ataacgggaa acagcataag cagtcacttc tgttaatgcg gttgctgaac
    12601 tgctgcgggt ttcttcttta aacatatcaa tcaaacctaa aatttgtaac tcgcgaacaa
    12661 catagcgacc tttaggataa gtaggtcctg ttacttccac ttctccggaa aaaataaaat
    12721 aaatcacttc cagaggatga tctttatcat aaagcaactc tccgctgtta aaattgcgct
    12781 tatgcaaaaa gttattgagc aaataaagct caaaagaact gagttcactt aaaacaggaa
    12841 acttttttaa gtcggcatat ttatccggtt ttttccagat tgttttcagc caatcaaata
    12901 catctttaag cacttttcac ctcaatgtct tatcatcaca aaccaaaggc acaaaagaac
    12961 aacctcctgc ttctgcaatg tccaatccgt cttctgtttt ggtaactata tacaaaattt
    13021 gaaaaccggt tttacctatg ggaattgcca ttttacctcc cattttcaat tgttccacta
    13081 aatgtttggg aacttcccgg gctgcagcag aaaccaatat tttatcaaat tccttatgtg
    13141 gaggatatgc tttttgccaa ccgctgtaac catcaccaat ccgaaaataa atattgttat
    13201 aaccggcatt gcgtaaaatc ttctgcgcat ttagggaaag ggtctccaac cgttctacag
    13261 tacatacctc tttaacaatt tctgcaagta gggcactttg ataaccacta ccggtgccaa
    13321 tttccaaaac aatatccgtg ggttgcaaat caagcaactg caacataatt gcgatcatta
    13381 aaggttgaga tatggtttga ccttctttta taggtaaggg acggttttga tatgcgtaat
    13441 cacgatattc aggtaaaaca taatgct
//
LOCUS       DAOPFL010000040        13147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5535_length_13147_cov_115.194241, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000040 DAOPFL010000000
VERSION     DAOPFL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5535_length_13147_cov_115.194241"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1101
                     /gene="recF"
                     /locus_tag="PLG69_04280"
     CDS             25..1101
                     /gene="recF"
                     /locus_tag="PLG69_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198307.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPI42761.1"
                     /translation="MNLAKIELENFRSYRQNEFDFNPQGCLIIGPNGCGKTNLLEAIA
                     YCSIGKSIRFHHDEELLNFEGQFFRVQSLFISDQQTPKKVSLSYADQHKLLKIDELPI
                     RQLSSLFEVVKVIYCAPEDHLLISGSPRFRRQYFDLAISQLYPPYINVLRHFLHIVQQ
                     RNAMLKRNYSRAEITSWNLSFASSLAEVWNYRNRYLKQVNNAFQETFKDIFPASTAIS
                     LAYIPSLKLPLESSPEEITKHLATIEEREKLLQRSLVGAHLDDYEFKLKGRKMLTYAS
                     QGQKRIAVIILKLIQARLIEKVTGIKPIMLFDDIFAELDTFHSQQIRNCINNRYQVFI
                     ASPKEDLCNIWQGYQIQKLEGIDK"
     gene            1098..2330
                     /locus_tag="PLG69_04285"
     CDS             1098..2330
                     /locus_tag="PLG69_04285"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPI42762.1"
                     /translation="MKFNRNIIGWMLYDFANSAFTTIIVTVVYSVYFINNVVGGPAGY
                     GEMLWGRAIGISMTLVAISAPIFGAVADYSRAKKRMLFINCYITVIFTALLYFVKPGD
                     VFIGMLFFIIANFGFNSANVFYDAFLPEICQPEDIGKVSGFGWSLGYVGGLVSLLVAL
                     VLVKINVRLVFPAVALHLFLFSLFTFFWLKEFRRPSQRSNYYRIAWQRVTYSLKNIAK
                     LPQLLKYIISYFIYNDGITTVIVFASIYGAERFGMTTPQMITYFILAQFTSILGAAFF
                     GWLTDKWNVKNSLSISLIIWIGVVIWAFFCSSASEYYFVGLVAGLAIGSSQANSRTML
                     SLLTPRDRQAEFFGFYTLTGRLSSIIGPILYGWIAHQTGDIRYSVLSLIFFFVIGWIL
                     LQSVQLQEGIEQAKVNEE"
     gene            2509..3567
                     /locus_tag="PLG69_04290"
     CDS             2509..3567
                     /locus_tag="PLG69_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HPI42763.1"
                     /translation="MKRGNIIVLIIVIVLLFFCGSCSKNKPVLYIFNWSDYIDPDLIK
                     EFEQQNKCTIKYSTYDSNENMLTKIMSSREAFDLVFPSGDHVTIMHEAGLLEPLDLTK
                     IPNYGNLDNNLLLKATSFDPGNKYSLPYFWGLTGIIYNQRYVPESVVKSQSWNILADN
                     FFTGKNKVTMLDDAREVVGAALIYNGYDLNDTSAEALAAAEKTLAEWDKNITQFDSDS
                     YKNEVPDGTTWLAQAYNGDALQVMENNPDIKFFLPVEGTSLWMDNIVMLKSSKNKELA
                     YKFIDFLLNAEIGKRNTEYCRYATPNKAANNLLPETIKNNALIYPSEEYLQKCSMINA
                     LGENVKKIDKLFEAIKLN"
     gene            3592..5373
                     /gene="aspS"
                     /locus_tag="PLG69_04295"
     CDS             3592..5373
                     /gene="aspS"
                     /locus_tag="PLG69_04295"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPI42764.1"
                     /translation="MLDNLGNLTRTHYCGNLNTEHIGQEVTVMGWVNKRRDLGGLIFI
                     DLRDVKGILQVVIRPEKPEIFAKAEKVRNEYVIAVRGKICARTEPNINPNLPTGKIEL
                     EAEEFYILNDAQPLPVQFSEVAMAEEDLRLTYRYLDLRRPKLQKIIITRHRIMQIIRE
                     FLNNEGFYEIETPILMKSTPEGARDYLVPSRMQPGKFYALPQSPQMFKQLLMIAGLDR
                     YYQIARCFRDEDLRADRQPEFTQLDIELSFVSQAQIFDLIERLFAKLFQEVLGYNLSI
                     PFPVIPYAEAMEKYGCDKPDIRFEMKLFDISNIVKNSGFNVFSSALKNGGVVKAIAIP
                     NAADFSRKQQDELVELAKHLGGKGIAFAKVTENGLEGGISKFLSPEEAEAIIQTTQAQ
                     KNDLLAFAADNYEMVSKVLAGIRNFLAPQLNLIPENSFAFCWITDFPLFTKNLETGKW
                     EPAHHMFTLPKEEHIPYLDIPEKIGEITGQLYDLVCNGVELSSGSIRCHRYDIQKKIF
                     DILGFSEEELQKRFGFFLEALKYGTPPHGGIAPGLDRLVMIMTGAESIRDVIAFPKTL
                     KATDLMSQAPSEVDEQQWKDLHLQPIK"
     gene            5340..5942
                     /locus_tag="PLG69_04300"
     CDS             5340..5942
                     /locus_tag="PLG69_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HPI42765.1"
                     /translation="MERPASATHKIAVFCSGLSRGSNLCALHNYFTQNKLPIEVALVI
                     FTRKDAPAVQLAEEKGLNYHIISTRNMQLFEQQAINLCQQHNIELIALAGFLKQLSEN
                     FIEEVQVPILNIHPALLPQYGGKGMYGMAVHKAVFASCDKFSGVTIHLVNSQYDKGKI
                     VAQQKVDISSCKSPEEIAEKVLEIEHAIYAPAICQFLLKS"
     gene            5939..7207
                     /gene="mtaB"
                     /locus_tag="PLG69_04305"
     CDS             5939..7207
                     /gene="mtaB"
                     /locus_tag="PLG69_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPI42766.1"
                     /translation="MIRIAIATLGCKANQAESSIILDQFNDFKLVPWQEEADIYIINT
                     CTVTNRTDYKSRYLIRQALSQKAQNPFAKIVVTGCFAQRYPEEIAKMGNIDWIIDNQQ
                     KLNIADILAGGNYEFMDIMQAKEFVYKPTAKMYNRTRAFQIIQDGCDFNCAYCAVPYG
                     RGRSRSATLEQIIQQAKLFVENGYKEIVLSGINLGLYKDGNNDLTDVLLRLNELQGLE
                     LIRLSSVEPQLFNDKLINTLPLIPKLCPHYHIPLQCGADSGLKRMRRHYTSTTINQLI
                     VKICERVPYPAIGMDVITGFPGETEEEHNQTCEFLRSLPLAYLHIFTFSKRKGTPAFD
                     FPNQISKTIKNRRANELSQISRELTSAYTKSLMENNIPLRGIVEKNTNGNCEFLSDHY
                     IRVRFSGNFQPGDFVQVPSQEAQIKMKNEE"
     gene            complement(7330..8109)
                     /locus_tag="PLG69_04310"
     CDS             complement(7330..8109)
                     /locus_tag="PLG69_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI42767.1"
                     /translation="MKSRQIILPLLFFVVLVLLWQLISTKGVIAFWVVPSPQQVIKVF
                     LDYPGLIWHHLKPTLIEAISGLLISIVLGSITAIAMNLSRLIKQIIYPYLIISQTVPI
                     IAVAPLLIIWFGYGISAKIFAVVLMCFFPIALGLYDGFRSVSVDQIRLLTSMGATPYK
                     IFRLLKIPSALPNFFTGLKLSATYSVMGAVIGEWLGGTSGLGIYMTRATKSYQTAHIF
                     AVIIVIVAVSLALFGIVALLDRILLKWKYHPIDEYLEPEKK"
     gene            complement(8106..9026)
                     /gene="murB"
                     /locus_tag="PLG69_04315"
     CDS             complement(8106..9026)
                     /gene="murB"
                     /locus_tag="PLG69_04315"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPI42768.1"
                     /translation="MLRKEYPQLIDEGIVHIEVPLKEHCSFNIGGPAEVFCTPYTQKQ
                     LVTVLKFCLEHNIPYFILGKGSNLLISDNGVKGIVISTERFDKITLGKHYLSAFCGVT
                     LKDLGNFACENGLSGLEFASGIPGSVGGAVFMNAGAYGNEIKDVLYSSRAILPTQEKL
                     NSPNPVLYLKASEHNFSYRHSVFQDLGLIHLSSLFILKKDKPENIRQRMQELQQKRQD
                     KQPMDLPSAGSVFKRPEGFFTGKLIEECGLKGFRIGDAAISEKHCGFIVNLGSATAKD
                     VFQLIKHIQKVVYERYGVHLQTEIRILGEI"
     gene            complement(9044..11926)
                     /locus_tag="PLG69_04320"
     CDS             complement(9044..11926)
                     /locus_tag="PLG69_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPI42769.1"
                     /translation="MESIDNEVKNTENFNNLPLEQLEFVAIDIETTGLNSEWDEIIQI
                     AGIRFKGEEVVNQFVSLVKPKGKVPKFIEHLTHISPQELKTAPPIKEVLKDFCNFIGD
                     SILVGHNIGFDFNFLNQFIVENGGFPINNMHWDTAEIGRIYLPFTTDHKLSTLVSFFG
                     IELMNAHQADADALATGKLFNALTNYILNHYGYMLNARLMDLAMQANLKSGLYEYIKW
                     IVRQQRSSALSGKKTVPVVNELNNVIEHIAAGSIKSIPEVFAEDGIFASKFPNFEFRS
                     GQMTMAEEVADAFGKQDFLVVEAGTGVGKSFAYLVPAIDFSNRNKSKVIVSTNTKNLQ
                     EQLFFKDLPQLKKILPLPFQAVLVKGRENYICERRWQELLQEQTRGITSYDAYGLLNL
                     LVWKEHTNTGDVSENSSFDKNRFGVLWHKICSDRYLCGGRKCPLFHSCYVMKLRKNIE
                     TASIVVANHSLLLADAQMDNSTLGEYQYLVIDEAHNLMSAASQNLGFTLSYVDLNSLF
                     NQLVYTSRKYSGGFLSQIMQVLQKSMLTTDKKTHIETLCTKLTGEIDSQRQVITKLFS
                     LAEDHCVEAKSYNKLRIKDINAFEDIYILLKELSLAFKDILKDLTALDNVFSTLNSNQ
                     VKDLDVLKDNLKGYLSRYMETEEIILQLLNPDLDNYALWIENDPISERNVPISTFCYA
                     PVEVGEHLNRLLYKNIPCIIFTSATLSIRGSFKFFLNQSGLSLVTEKNVRQSIVESPF
                     DYDKQSLLLIGSFLPEHKDKFFQSQALGCLEQILTTTNVGTMALFTSYKDLDAVYNHL
                     SDTLFHLNRPFFAQGKGVSRNSLLDEFKKSKNAVLLGTSSFWEGVDIQGDSLSLLILY
                     KIPFQVPSEPLVEALIDKLERENKDSFMHLMLPNALLRLRQGFGRLIRSKTDRGVVLI
                     MDSRVSKKKYGIYFKQILPGRCIELKDEQHLISEIGKFFNPLPANI"
     gene            complement(11942..>13147)
                     /locus_tag="PLG69_04325"
     CDS             complement(11942..>13147)
                     /locus_tag="PLG69_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPI42770.1"
                     /translation="KQPHFAPSYNNILALPLSIRKNTMGVVLLFCKSGSKYFSERIIN
                     LLSALSNQIIVIIENILSANLEKSHNVIREQLSSNNIYSNIIGKSPEMMKIFEIIEKV
                     KDTPTTVLLEGPSGTGKELIARALHYSSNRRNKAFVAQYCGALPETLLESELFGHVKG
                     SFTGAAYDKKGLFEIADGGTFFLDEISDISQSTQSKLLRFLQEGEIKRVGATKTEKVD
                     VRVVCATNTPLMERVKSGEFRLDLYYRLNVIRIDVPPLKNRTGDIPLLAIHFLDKYNK
                     RMNKNVMGFTAEAMKVLEQYDWPGNVRQLENEIERAVTLAEPNTFIKPSDFSEEVYRH
                     IEYSKTIAMLSGKKNLKDAIEELERKLISQSLEKFQWNQTQAAKELGLSRQGLIKKMQ
                     RYNLYREED"
BASE COUNT         4089 a         2599 c         2455 g         4004 t
ORIGIN      
        1 tgatgccttt gcgttcataa aaatttgaac ctggctaaaa ttgaacttga gaatttccgc
       61 agctatcgcc aaaatgagtt tgatttcaac cctcagggct gtttgataat tggtcctaat
      121 ggctgtggaa aaaccaatct cctggaggca attgcctatt gcagtatagg taaatccatt
      181 cgttttcacc acgatgaaga actgctcaat tttgaagggc agtttttccg tgttcagagt
      241 ttatttatca gtgaccagca aaccccgaag aaggtctctt taagttatgc agatcagcat
      301 aaacttttaa agattgatga attgcctatc cgccaattaa gttcactttt tgaagtggta
      361 aaagttattt attgtgctcc agaagaccat ttattgataa gtggttcacc gcgttttcgc
      421 cgacaatatt ttgacctagc aatttctcag ctttatcctc cttacatcaa tgtcctaagg
      481 cattttttgc atatcgttca gcagagaaat gcaatgctga aaagaaacta ttcccgggca
      541 gaaataacca gctggaattt atcttttgcc tcttctttag cggaggtctg gaattatcgg
      601 aatcgctatt taaagcaggt taataacgct tttcaggaga ccttcaaaga tatttttcct
      661 gcttccactg ccatttctct ggcttatata ccttctttaa aattgccttt ggagagttct
      721 ccggaagaaa ttacaaaaca cctggcaact attgaagaaa gagaaaaatt actacaacgc
      781 tctttagtcg gagctcattt ggatgattac gaatttaagc ttaagggtcg taaaatgctt
      841 acctatgctt ctcaagggca aaaacgcata gcagttatta ttcttaagct cattcaagca
      901 cgcctaattg aaaaagttac cggcataaaa cctattatgc tttttgacga tatttttgcc
      961 gagttggata cttttcattc tcaacaaata agaaattgca tcaataaccg ttatcaggtt
     1021 tttattgcca gtcccaaaga agatctctgt aatatctggc agggctatca aattcagaag
     1081 ttggaaggaa tagacaaatg aaattcaacc gtaatattat cggctggatg ctctatgatt
     1141 ttgccaattc tgcctttaca actataattg ttacagtcgt ttacagcgtt tattttatca
     1201 ataatgtagt aggcggtcct gccggttatg gagaaatgct ttggggaaga gcaatcggaa
     1261 tttctatgac cctggttgct atttcagcac ctatttttgg agctgtagca gattattccc
     1321 gtgccaaaaa acggatgctt ttcattaatt gctatataac cgtaattttt acggctctgc
     1381 tttattttgt taagccaggt gatgttttta tcgggatgtt gtttttcatt attgccaatt
     1441 tcggtttcaa tagtgctaat gtattttacg atgctttttt gcccgaaatt tgccagccag
     1501 aagatatcgg taaggtttcc ggttttggct ggtctttagg atatgtaggt ggtttagttt
     1561 cccttcttgt agctttagtt ttggtaaaaa tcaatgtgcg tttggttttt cctgctgtgg
     1621 cattgcacct gtttttattt tccctgttca cttttttctg gttgaaagaa ttccgtcgtc
     1681 cctctcaaag aagtaactat tatcggattg cctggcaaag agttacttat tcactaaaga
     1741 acattgccaa gttacctcaa ctgctgaaat atattatcag ctattttatt tataacgatg
     1801 gcatcactac agtgattgtt tttgcttcta tttatggagc ggaaaggttt gggatgacaa
     1861 ctccgcagat gataacctat ttcattctgg cacaatttac ttccattttg ggagcggcat
     1921 ttttcggttg gcttacggat aaatggaatg taaaaaattc cttgagtata tcccttataa
     1981 tttggattgg cgttgtaatc tgggcatttt tttgcagcag tgccagtgaa tattattttg
     2041 ttggtctggt tgccggtttg gcaattggca gtagtcaggc aaatagtaga actatgcttt
     2101 ctcttttaac tccgagggat agacaagcag aatttttcgg attttatacc ttgacaggac
     2161 gcttatcttc tattattggt ccaattttat atggatggat agcacatcag acaggggata
     2221 ttcgttattc cgttttgtcc ttgatttttt tctttgtgat tggctggatt ttattgcaga
     2281 gtgtgcagtt acaggaaggg attgaacaag cgaaagtaaa tgaagaatga agtggaagac
     2341 aaaaaaaata gaacgcagat aactggagcg acttgattag caggatttta cataatggta
     2401 attaaaaaac taaaatctgt gttaatcagc taaaatccgt taaatccgtg tgctaatctt
     2461 ttatctgggt tatctgtgta atctgtgaga gaaaaaaagg agagaaagat gaaaagagga
     2521 aatatcatag tcctaataat agtcatagtt ctgctgtttt tttgcggttc ctgcagtaaa
     2581 aataagccgg ttctgtatat cttcaactgg agtgattata ttgatcccga cctcatcaag
     2641 gaatttgaac agcagaataa atgcactatc aaatatagca cttacgattc caacgaaaat
     2701 atgctcacca aaattatgag ttcccgcgaa gcatttgatc ttgttttccc cagtggtgat
     2761 catgtaacta ttatgcatga agcgggttta ctggaaccgt tggatttaac caaaattcca
     2821 aattacggca atctggataa caatcttttg ctgaaagcaa ccagttttga ccctggcaat
     2881 aaatattccc taccctattt ttggggttta accggaatta tttataatca gcgttatgtt
     2941 cctgaaagcg ttgttaaatc ccaaagctgg aatatcttag ccgataattt tttcaccggt
     3001 aaaaacaaag ttacgatgct ggatgatgca cgcgaagttg ttggtgccgc attaatctac
     3061 aatggctacg atcttaatga tacaagtgct gaagcattag cggctgcaga aaaaaccctt
     3121 gccgaatggg ataaaaatat tacgcagttt gattctgata gctataaaaa cgaagttccc
     3181 gatggaacaa catggctggc tcaagcatat aacggagatg ctttacaggt tatggaaaac
     3241 aatcccgata ttaaattctt tctccccgtg gaaggtacca gcttatggat ggataatatc
     3301 gttatgctta agtcctctaa aaataaagaa cttgcctata aattcattga ttttctccta
     3361 aatgcggaaa tcggtaaacg caatactgaa tattgccgct atgccactcc caataaggca
     3421 gcaaataatt tgttacctga gacaattaaa aataatgcac ttatctatcc aagcgaggaa
     3481 tatcttcaaa aatgctctat gattaatgcc cttggcgaaa atgtgaagaa aatagataag
     3541 ctctttgagg caatcaaatt aaactgataa agcgagaaaa aagaaaacac aatgcttgat
     3601 aacctgggaa atttaacccg cactcattat tgcggaaatt tgaatacgga gcacattgga
     3661 caagaagtaa ccgttatggg ttgggtgaat aaacgccgcg acctgggtgg tttaattttt
     3721 attgacttgc gtgatgttaa aggtattctg caagttgtta tccgtcccga aaaaccggag
     3781 atttttgcca aagcggaaaa agttcgtaat gaatatgtaa ttgctgtccg cggaaaaatt
     3841 tgtgcgcgca ccgaacccaa tattaatccc aatcttccca ccggcaaaat tgaactggaa
     3901 gcggaggaat tttatatctt aaatgatgca caaccccttc ccgttcaatt tagtgaagtc
     3961 gctatggcgg aagaggattt gcggcttact tatcgctatt tggatttgcg tcgtcccaaa
     4021 ctgcaaaaaa taattatcac caggcaccgc ataatgcaaa ttatacggga atttctcaat
     4081 aatgagggtt tttatgaaat tgaaactccc atcctgatga aaagcactcc tgaaggagca
     4141 agggattatc ttgttcccag tagaatgcaa ccgggaaaat tttatgcctt accgcaatcc
     4201 cctcaaatgt ttaaacagct cttaatgatt gccggcttgg atagatacta tcaaatcgcc
     4261 agatgcttcc gagatgagga tttaagagca gatagacagc ccgaatttac gcaattggat
     4321 atagagctga gtttcgtttc tcaagcgcaa atctttgatc ttatagaacg cctttttgcc
     4381 aaactttttc aggaggtctt gggatataat ttaagcattc cttttcctgt tattccttat
     4441 gcggaagcaa tggagaaata tggctgtgat aaacccgata ttcgttttga aatgaagtta
     4501 tttgacattt ctaacatagt gaaaaatagt ggttttaatg tcttttcttc cgccctgaaa
     4561 aacggaggag tagttaaagc aatcgccatt cctaatgcag ctgatttcag cagaaagcaa
     4621 caggatgaac tggtagaact tgctaagcac ttgggaggta aaggaattgc ctttgccaaa
     4681 gtaacggaaa acggtttgga aggtggcatc agtaaattcc ttagccctga agaagcagag
     4741 gcaattattc aaactaccca agctcaaaaa aatgaccttc ttgcttttgc tgcagataat
     4801 tatgaaatgg taagcaaggt tttagcagga attcgtaatt tcttagctcc ccaattaaat
     4861 ctcattcccg aaaacagttt tgccttttgc tggataacgg attttcccct ctttactaaa
     4921 aatctggaaa caggtaaatg ggaaccagct catcatatgt ttaccttacc taaagaagaa
     4981 catattccct atctggatat tcccgaaaaa atcggtgaaa ttactggaca actttatgac
     5041 cttgtctgca atggagttga actttccagc ggcagtatca ggtgtcatcg ttatgacatc
     5101 caaaagaaaa tatttgatat tcttggtttt agtgaagaag aactgcagaa gcgtttcggg
     5161 ttctttctgg aagcactaaa atatggaact ccaccacacg gaggaatagc tcctggtttg
     5221 gatagattag ttatgattat gacgggagct gaatccatcc gagatgttat tgctttccct
     5281 aaaacattga aggcaacgga cttaatgagc caggcacctt ccgaagtaga tgaacagcaa
     5341 tggaaagacc tgcatctgca acccataaaa tagctgtttt ttgcagcgga ttaagtcggg
     5401 gctcaaattt gtgtgcattg cataactatt ttacacagaa taagttaccc attgaagtcg
     5461 ctttggtcat ttttacccgc aaagatgcac ctgcagttca attggctgaa gaaaaaggat
     5521 tgaattacca cattatttct acccgcaata tgcaactttt tgaacaacag gcaataaacc
     5581 tttgccaaca acataacata gaactgattg ccttagcggg ttttttaaaa cagctctcgg
     5641 agaattttat tgaggaagtt caagtcccca ttttaaacat ccatcctgcc ttattgcccc
     5701 aatacggagg caaaggtatg tatggaatgg ctgtgcataa agcggttttt gcttcctgtg
     5761 acaaattttc cggtgttact attcatcttg ttaattccca atatgataag ggaaaaattg
     5821 tagctcagca aaaagtggat atcagttcct gtaaaagtcc ggaagaaatt gctgaaaaag
     5881 tgctggaaat tgaacacgca atttatgcac ctgccatctg tcaattcttg ttaaaatcgt
     5941 gattcgcatc gctattgcta cccttggttg caaagccaat caagccgagt cctccataat
     6001 tttagaccag ttcaacgact ttaagttagt tccctggcag gaagaagcgg atatttatat
     6061 catcaatacc tgcactgtaa cgaaccgaac cgactataaa agccgctatt taatccgtca
     6121 ggcattatct caaaaagcgc aaaacccctt tgccaaaatt gttgtaaccg gctgctttgc
     6181 tcaacgctat ccggaagaaa ttgccaaaat gggcaatatt gactggatta tagataatca
     6241 gcagaaactg aatattgcgg atatccttgc cggtggcaac tacgaattta tggatattat
     6301 gcaggcaaag gaatttgtct ataagccaac tgctaaaatg tataaccgca cacgcgcctt
     6361 tcaaattatt caagacggct gcgattttaa ctgtgcttat tgtgcagttc cttatggaag
     6421 gggcagaagc aggtcggcaa ctttggaaca aattattcag caagccaagt tatttgtgga
     6481 aaacggttat aaggaaattg tgctatcagg aattaactta ggactataca aagatggaaa
     6541 taacgattta accgatgttt tgctccgctt aaacgaattg cagggcttgg aattgattcg
     6601 ccttagctcc gttgaaccgc aattgtttaa tgataaacta attaacactc ttcccttaat
     6661 tcccaagctt tgcccgcatt atcatatacc gttgcaatgt ggtgctgatt ctgggctaaa
     6721 aaggatgaga cgccactata cctccaccac aattaaccaa ctaatagtta agatttgtga
     6781 acgcgttccc tatccggcaa tcggaatgga tgtaataacc ggttttcctg gtgaaactga
     6841 agaagaacat aaccagacct gtgaattttt gcgctcttta cccctggctt atcttcatat
     6901 tttcactttt tctaaaagaa aaggaacccc tgcttttgat tttcctaacc aaatctctaa
     6961 aaccattaaa aaccgcagag ccaatgaact cagccaaatt tcccgcgaac tgacttccgc
     7021 ctatactaaa tccctaatgg aaaataatat cccgttacgc ggaattgtgg aaaaaaacac
     7081 caacggtaac tgcgaatttt tgagcgatca ctatatccgc gtccgctttt ccggaaactt
     7141 ccaacccggc gattttgtcc aagttccaag ccaagaagca caaataaaaa tgaaaaatga
     7201 agaatgacta agagaattca gataaccgga tctactgggt cagccgtatt tcaatataag
     7261 gataattata aataaaaaaa tttgcggtat cggcgaaatc tgcgtgcact tttttttatc
     7321 tgtgagaggt tatttttttt cgggttccag atattcatca ataggatgat atttccattt
     7381 cagcaaaatc ctatccagca aagccacgat tccgaacagc gctaaactga cagccacgat
     7441 aacaataatc acggcaaaaa tatgagctgt ttgataggac tttgtagctc tggtcatata
     7501 aattcccaag ccggaagttc cacctaacca ttcacctatc acagctccca tcacgctata
     7561 agttgcagat agtttaagtc ccgtaaaaaa attgggcaaa gcggaaggaa tttttaataa
     7621 tctaaatatc ttatagggtg ttgctcccat tgaagttaaa aggcgaattt ggtctacaga
     7681 cacactcctg aaaccgtcat aaagtcctaa ggcaatagga aaaaagcaca tcagcactac
     7741 agcaaaaatt ttagcactga tgccataacc aaaccaaatt ataagtaaag gcgctacagc
     7801 tataatagga actgtttggg aaataatcag ataaggatag ataatttgtt tgataaggcg
     7861 ggataaattc attgcaatag ccgttataga gcccagaaca atactgatta acagtccgct
     7921 aattgcttct atcaaagttg gctttaagtg atgccaaatc aaacctggat aatcaagaaa
     7981 aaccttaatc acctgttgcg gcgaaggaac aacccaaaaa gcgataactc ctttagtgct
     8041 gataagttgc cagagcaaaa ccagaaccac aaaaaaaagc aaaggcaaaa ttatttgccg
     8101 gcttttcata tttctcccaa aatacgaatt tccgtttgca gatgaacacc atagcgttca
     8161 taaacaactt tttgaatatg cttaataagc tgaaaaacat ctttagcagt agcagaacca
     8221 agattaacaa taaaaccgca atgcttttcc gaaatggctg catcaccaat ccgaaagccc
     8281 ttcaatccgc attcctcaat taattttccg gtaaaaaaac cttccggtcg tttaaaaacg
     8341 ctgccggcac tgggtaaatc cattggttgt ttatcctgtc gtttttgttg taattcctgc
     8401 attcgctggc ggatattttc cggtttatct tttttcagaa taaacagaga ggaaagatga
     8461 attaagccca aatcctgaaa gacgctatgc cgataggaaa aattatgttc tgacgccttc
     8521 aggtataaaa cagggtttgg agaattgagc ttttcttggg taggcaaaat agctctgctg
     8581 ctgtaaagaa catccttgat ctcgtttccg taggcacctg cattcataaa aacagcccct
     8641 cctacagagc ccggaattcc actggcaaat tccaatccgc ttaatccatt ctcacaggca
     8701 aaattaccta agtccttaag ggtaactcca cagaaagcag ataaatagtg cttacctaaa
     8761 gtgattttat caaaccgttc cgtgctgata actattccct ttactccatt atccgatatg
     8821 agtaaattag agcccttccc taaaataaag taaggaatat tatgctcaag gcaaaacttt
     8881 aagacagtaa ctaattgttt ttgggtataa ggagtgcaaa aaacctcagc gggaccccct
     8941 atgttaaaac tgcaatgttc ttttaaggga acttctatat gcacaatgcc ctcatcaatt
     9001 agttgagggt attctttccg taacaaaaga aaagtatcca tactcaaata ttggcaggta
     9061 aaggattaaa aaatttaccg atttcagaaa tgaggtgttg ttcgtctttc aattcaatac
     9121 agcgtcctgg taatatttgt ttaaagtaaa tgccatattt ctttttggaa acacgggaat
     9181 ccataataag aactactccc cggtctgttt tactgcggat taagcgtcca aaaccctgtc
     9241 ttaaacgcaa taaagcattg ggcaacatca gatgcataaa ggaatcctta ttttcccgtt
     9301 ccagtttatc tataagtgct tctaccaatg gctcggaagg cacttgaaaa ggaattttgt
     9361 ataaaatcaa tagagaaagt gaatcccctt gaatatccac cccttcccaa aaagaagaag
     9421 ttcccaataa cacggcattt ttactcttct tgaattcatc cagaagggaa tttctgctta
     9481 caccctttcc ctgagcaaaa aatggacgat tgagatgaaa aagggtatcg ctaagatgat
     9541 tgtaaacagc atccaagtcc ttataggaag taaaaagtgc catcgttcct acatttgtag
     9601 tagtcaatat ttgttccaaa caacccaatg cctgactttg gaagaattta tccttatgct
     9661 caggaagaaa actgccaatt aacaataagg actgcttatc ataatcaaaa ggtgattcca
     9721 caatactctg cctaacattc ttttctgtta ccaaactcaa gcccgattga ttgaggaaaa
     9781 acttgaaaga acccctgata gataaagtgg cagaagtgaa aataatacag gggatatttt
     9841 tataaagcaa acggttaaga tgttctccaa cctccacagg tgcataacaa aaagtgctga
     9901 taggaacatt gcgttccgaa atgggatcgt tttcaatcca taaagcataa ttatccaaat
     9961 cgggattaag aagttgtaaa attatctctt ccgtttccat atagcgggat aaataaccct
    10021 tcaaattatc tttcagaaca tccaaatcct tcacttgatt gctgtttaaa gtgctgaaaa
    10081 cattatccag ggcagtaagg tctttcaaaa tatccttgaa agcaagagat aattccttca
    10141 gaaggatata gatatcttca aaagcattga tgtcctttat ccgcaattta ttatagcttt
    10201 ttgcttcaac acaatggtct tccgctaaac tgaaaagctt tgttatcacc tgcctctgac
    10261 tgtcaatttc ccctgtcagt ttggtgcaaa gggtttctat atgcgtcttt ttgtcagtgg
    10321 ttaacatact cttctgcaat acctgcataa tttgggaaag aaagcccccg gaatatttcc
    10381 tggaagtata aacaagctga ttaaataagc tgttgaggtc aacataactt aaagtaaaac
    10441 ccagattttg ggaagcagcg gacatcagat tatgcgcttc atcaattacc agatattgat
    10501 attcacctaa agtggaatta tccatttgag catctgccag cagcaatgaa tgattagcaa
    10561 ctacaataga tgctgtctct atattcttac gcaatttcat tacataacag ctatggaaca
    10621 agggacactt tctgcctcca caaagatagc gatcggaaca tatcttatgc cataaaacgc
    10681 caaagcggtt tttatcaaaa gaagaatttt cggaaacatc acctgtattc gtatgttctt
    10741 tccaaaccag aagatttagt aaaccatacg catcgtaaga agttatgcct cttgtttgtt
    10801 cctgtaaaag ttcctgccac cttctttcgc agatataatt ttcccttcct ttaactaata
    10861 cggcttggaa aggcaggggc aatatttttt tcagttgcgg taaatccttg aagaaaagct
    10921 gttcctgcag atttttagta ttggtagaaa caattacttt gctcttattc cggttactga
    10981 aatcaattgc cggaaccaga taagcaaagg attttccaac tcccgttcct gcttctacca
    11041 ccaaaaaatc ctgtttgcca aaagcatcgg caacctcttc tgccatagtc atttgtccgg
    11101 aacgaaattc aaaattggga aatttggagg caaaaattcc atcttcagca aagacctcag
    11161 gaatgctttt tatagaacct gcagcaatat gctcaataac attgttcagt tcattgacta
    11221 caggaactgt ttttttaccg gaaagagctg atgaacgctg ttgtctcaca atccatttga
    11281 tatattcata caatccgctt ttcagatttg cctgcattgc taaatccatt aaccgggcat
    11341 taagcatata gccataatga tttaaaatat aattggttaa ggcattaaag agctttcctg
    11401 ttgccagggc atctgcatct gcctgatgag cattcattag ttcaattccg aaaaaagaaa
    11461 ccagggtgct taatttatgg tcagttgtaa aaggtaaata aatacgtcct atttctgctg
    11521 tatcccaatg catattgttg attgggaaac ccccgttttc cacgataaat tgattgagaa
    11581 agttaaaatc aaaaccaata ttatgtccca ctaaaatgct atcgccaata aaattacaaa
    11641 aatccttcaa tacctctttt ataggaggtg ccgtttttag ttcctgaggc gaaatatgag
    11701 ttaaatgctc aataaatttg ggcactttcc ctttgggctt aaccaaactg acaaactgat
    11761 taacaacttc ttcaccctta aagcgtatac cagctatctg aataatttca tcccattccg
    11821 aatttaagcc ggtggtttca atatctatag ctacaaattc caattgctcc aaaggcaggt
    11881 tgttaaaatt ctctgtgttt tttacttcgt tgtctatact ttccacattt tatcctattc
    11941 gttaatcttc ttcgcggtaa agattataac gctgcatctt tttaattaag ccctgacgcg
    12001 aaagccccaa ttctttagct gcttgggttt gattccactg aaatttttcc agggattgag
    12061 aaattaattt gcgttccagt tcttcaatgg catcctttaa gtttttcttt cccgaaagca
    12121 tagcgattgt tttgctgtat tcaatatgac ggtaaacttc ttcactaaaa tcactgggtt
    12181 taatgaaagt attgggttca gccaaagtta ctgctctttc aatttcattt tccagctgac
    12241 ggacatttcc aggccagtca tattgttcca aaaccttcat tgcttcagct gtaaatccca
    12301 taacattttt attcattcgt ttattgtatt tatccagaaa gtgtattgcc aaaagaggaa
    12361 tatcacccgt tctgtttttc agcggcggaa catcaattct gataacattt aaacggtaat
    12421 agaggtctaa acggaattct ccgcttttaa ctctttccat taaaggcgta ttagtagcgc
    12481 aaacaactcg cacatcaacc ttttccgttt ttgtagctcc aactctttta atttctcctt
    12541 cctgcaaaaa acgcagcagt ttggactgtg tggactggct gatatcggaa atttcatcca
    12601 gaaagaaagt tccaccatca gctatttcaa ataaaccctt tttatcataa gcagcacctg
    12661 taaaagaacc ctttacatga ccaaaaagtt cactttccag taaggtctcg ggcaaggcac
    12721 cgcaatattg agctacaaat gccttatttc ttctgttact gctatagtgt aatgctctgg
    12781 ctatcaattc tttacctgtt ccgctgggac cctctaaaag cactgtagta ggcgtatctt
    12841 tcaccttttc aataatctca aaaatcttca tcatctcagg gcttttacct attatattgg
    12901 aatagatatt attggagcta agctgttcgc ggataacatt atggcttttt tccagattgg
    12961 cactgaggat gttttctata attacgataa tttgattgga aagagcggaa agcaaattga
    13021 taatcctttc ggaaaaatat ttggaaccgc ttttacaaaa gagcaaaacc actcccatag
    13081 tatttttacg gatggaaaga ggcagagcta aaatattatt ataactggga gcaaaatgcg
    13141 gctgttt
//
LOCUS       DAOPFL010000041        13107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5558_length_13107_cov_80.941235, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000041 DAOPFL010000000
VERSION     DAOPFL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13107
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5558_length_13107_cov_80.941235"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..3428)
                     /locus_tag="PLG69_04330"
     CDS             complement(276..3428)
                     /locus_tag="PLG69_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPI42771.1"
                     /translation="MNRKVLFLFLLFLIGIFFLCWGEQITIGNPASATYGLIPINRHH
                     NYSTYEMLYTSGELRSECLINKIAFYKRRGNPSLLISNVSIYMKVSSATSLSEGEISP
                     PYSEYQLVYSGNFPNNFGSSGWAEVTLFDPYPYNATGNLHILVVKGFEVWSTDRPDYT
                     YSSTGSTYLCRGSYSDNFQPTYLTPTYNRPVIRFDAEFLIENEPPLPPINPFPANSAI
                     DVLKNITLHWRSGGGCPSHYDVYFGDTSNPVKVISLLNETNFNPGILEYGKTYYWKID
                     PYNIYGYASDKYELEVWSFTVMNDPTITRNDLPYLQTFDNEWSGSPPSPLGWIVINAN
                     NDDYTWRQTNECNIPAYSEPYAAQGMGNTDDYLISPPLDLTAVNIKIKWYDMVESAQY
                     PNSYKVLLSTTDSDISSFTEELAVFTCYNTEWTEHSLDLSTYRDHHIYLAFYQYASTA
                     TNWSFGIDDFQIEKLPDVPILNYSPTEIIFPFTPVNTPSSYLNVTIANTGEGIIDLTE
                     DNFTLLGDYDDFEVDYSNLDAHLETDQSVNIPVRFYPRSFGEKNAILRINYSSRTQYD
                     VILKGTTYFEGLLMEDFEDDFLPVGWTKYVNNNNDIIQSNDNNHTFNGQFSARFSSLE
                     VSNDYNQYLFSPPVLINAFHHNLSFWHLKQNSEETLEWGIATDTNPENYDWKPVNLSC
                     VSWQKTQMDLKDYADQMVYLGFHYYGNNSQSVYLDDVHISCDIPANYPTEIDGITITS
                     TIHLNINNAVTATHPLVMALPNYNNLNAPIVLGLLGSGKGNLSLKLSSGNWYCLIYHS
                     GQWHYGSSYPCNITEGNTGIVNISNVEFGAKNDVILVIEEGIDPTLPVNLSRFTAVVT
                     SENFVIISWTAESETNHSGYNILRSEDKDLQNAIMINGSIINKGSSEGTQIKYNYTDF
                     EVYTNMIYYYWLESVSLDGNSEFYGPITVIIGDPTQEPVPPTVPMVTKLYNAFPNPFN
                     PNTNIRYSLKEAGKVKIEIYNMKGQKIKTYDQEHTSPGYYQVSWDSCDEKGRSVASGI
                     YLYRLSTGNYTSAKKMVLAK"
     gene            4589..8173
                     /locus_tag="PLG69_04335"
     CDS             4589..8173
                     /locus_tag="PLG69_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42772.1"
                     /translation="MKNIALLAILLFSTAVLIAGVTMNYSLETPKIEAKEQYTKVSLE
                     KAQSWGQPGNPDLPWLGIKLLLPAGFEAEKIVVNRYNPVTYTLDKPIAPIQRQYPFSH
                     IILETPDKPNAEVYDSPLPYPENTDNGLITSFMCGQPIAFSAISPFSYYPLTNELIFY
                     RQLSVEIIYSSSAKAMEAMRFLKQDSFTANRLQKIVDNPETITISSTRETGIEYLIVA
                     DAEKITQWTPLKTFQETRGKTVFIKSMSEIIAENQGIDTQDKLRNYIISLYTTNPLRY
                     VFLAGDTDVIPHRGFYVNVNNSDEGTDADIPADMYYACLDGNWNTDGDSYWGEMYEAD
                     LTPELAIGRFCYNSDAEIVNFLNKLTNYSNNPVANEVKSAFFVGEWLWDGPTWGGDYM
                     DEMIGGSSNNGYTTVGVPTSWNITTLYDRTYGAADAWGANQIRPMLSQGPNLVNHLGH
                     SNTTYAMRLSNNQVSASTITNDGVNHNFSTYFTQGCYAGAFDNRETSPGQYTSDCITE
                     KMTSIATGPVAMISHSRYGWGVQQSTDGASQYIHRQFIDAIFGENIYEAGFALVDSKI
                     DNIPYITNEPVMYWVTYETNLFGDPAMMVWTDTPQTITANLPEVLMVGQNHYQIQTNA
                     PYAVFILKNEDHTVYRTSADENGLIIVNFFAPLTPGTYYIYISAHNFYPFNATITATA
                     AQMPYIICNNVSYNDQDGLYHTGETLSLNLFVKNVGLMDLTVPGTLTFLSNSANIQIL
                     SSTANFTGIAAGDSLALNSVFLFRIVGNFTDQTQATFTIRANYDSYLSNSTFILTLNA
                     PVLQLLSYQLNNMDVMIMPGDTPSISFSVANTGSGNAYTPMLIIFNSSPDIILEQDEI
                     LLAPLSPHSTADYQSAITLQVLDSAVIDNNYMINYIFSAENGNLVEGNFLIHIGALSY
                     SFETDYQTWETVALSSNFVNQWHRSSNRNHTTNGIYSMKFGGNGSSNYASSAYGALIS
                     PVMNVSPNCQLKFYHWMSAEKHSTNQSYAWDGGLVQMSLNGGNWTQITPVGGYPYKIY
                     NNSASPFSANTWVYSGNFDWTEAIFELGNVSGIAQFRWVFGSDGYVGGEGWYIDDVRI
                     TGYVANEDESIPLAQNVILYDNYPNPFNPETTIEFSIPARMPVCLEVFNIKGQLVTRL
                     IDEVLPAGTHKVVFNGLDINKRNIASGVYYYRIKTTSGVQMRKMLLLK"
     gene            8176..8889
                     /locus_tag="PLG69_04340"
     CDS             8176..8889
                     /locus_tag="PLG69_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPI42773.1"
                     /translation="MLKFTPDTNVLVLTGAGISAESGIRTFRDSGGLWENHRVEDVAT
                     PEAFNSNPVKVWEFYRQRYQDSISSKPNPAHFALVKLEQFLGDNFLLVTQNVDCLHIK
                     AGSKRVLEMHGSLANCICTKCHSKLAIKDIDLTQPIPLCPFCNGFLRPDIVWFGEIPH
                     YLSIIDDALRKCSVFIIVGTSGVVYPAAGFVLTAKLMGAKTIAINLEKPDNLAYIDEF
                     YQGKSGEILPGLVAMWIGE"
     gene            9213..10502
                     /locus_tag="PLG69_04345"
     CDS             9213..10502
                     /locus_tag="PLG69_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPI42774.1"
                     /translation="MRKFFIMIIMLFVLLAGCKSALQSNKVKVAFWHGLSGPLGDTLN
                     EMIREFNHTHKDIEVIANPISSYTALSQKLMASIQAKKQPDIAQVFESWTSKYIEAGV
                     LCNFEDLMKADSTWTMQSLEDIYPVFLKSNTFNDTIYSFPFNKSVRAYFYNKDAFYRA
                     GLDPNHFPATWDEFRSYCRKLTTDKNNDGKPETYGTNFNVNEWQFINLLYQAGGTILD
                     EKGNPQLNSPQAIEALTFTTDLLNKDKTVYLVREFEGQNDFLAGIAAMYEGSSVSITH
                     MRQQPINFNIGYAPLPVYRTNKSAVSGTNIVIFKSGDRKREQAAWEFIKWFTDTEQTA
                     RWSAETCYMPVRKSAMQSPVLQNFLKEYPQFQGIYDQLETAIFEPQISAWFKARMELK
                     NYLEKAMRGISTPQEALDEMNAKFQAIIEEEKYLKSL"
     gene            10524..11171
                     /locus_tag="PLG69_04350"
     CDS             10524..11171
                     /locus_tag="PLG69_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HPI42775.1"
                     /translation="MILNCAEELILLSIDDQTNSFYRITNINFNVALIGALLMDLALR
                     KRIDVDLEGIYVLSTEPTGDKFLDAILENLIETEAGNQPAVLVGQLYNSMGHLKDKLL
                     QDLEEKGIIKVSQCKVLWLFKKRRYPIIDQKEEEEVLSRIRKTVLTDVDPEPRDLALI
                     ALLNICNLMDKIFTQEELEQRSERLEQLKKLDLISPAVKRIIGEMQVLITTTYTT"
     gene            complement(11349..11495)
                     /locus_tag="PLG69_04355"
     CDS             complement(11349..11495)
                     /locus_tag="PLG69_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42776.1"
                     /translation="MLYHLSYARFFIELKNLDGVYEVKEKNNEVANSVLPQRMQSFIL
                     NNAA"
     gene            11469..11544
                     /locus_tag="PLG69_04360"
     tRNA            11469..11544
                     /locus_tag="PLG69_04360"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11502..11504,aa:Gly,seq:ccc)
     gene            complement(11732..>13107)
                     /locus_tag="PLG69_04365"
     CDS             complement(11732..>13107)
                     /locus_tag="PLG69_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gingipain R"
                     /protein_id="HPI42777.1"
                     /translation="LRATSGNGIVTLTWQSPMAGNIDSYNIYRNNALYDNVANRYTYY
                     DVDVVNGTTYSYYLTSVSGINESLPSLTVQATPSSSSVSETIIGTGTSITGTNTASPV
                     NIYYKSLHGQSVYTAAELNAAGIYGPIDILQLGFYIASSPAYALPNFLIRMKHTTAID
                     VSSWQTATGMTTVYTNASYMPVAGGWDMLTLTSPFHWNGIDNIVIDTAFGLVSQWNSS
                     GTVQYTSVNNGYRYTWSDSSDQTNVFTGSSTSSNRPNIKIRVPSEPQNAIISISSSQL
                     NFGAIEIGNSATLPFTIQNTGNQLLAGYFTVPNNYTLDFAPRNKNKDLQETRYSDRTN
                     MQFAIEAGNSLTVNVTFTPTAQTSYNGNLVITSNAVNSHNFNLVLNGSGYYPSLETPI
                     VTISREYNNIVLRWNAVPNALSYQIYRSDNPYSNFTLIGTTSYLQFTDASGNQGFYYI
                     KASTQHP"
BASE COUNT         4206 a         2406 c         2414 g         4081 t
ORIGIN      
        1 gagcttcggc aagctcctgc tacacaaaaa aaacgagctc cgaatacaca agattttgtt
       61 gacaaggata tcaactttct caaccctctc aactttctaa accatctcaa ccttatcaac
      121 atttttttaa cgaagggctt acgccaccat cgctgccatt gtttcgccct ctgagctttt
      181 atagaatctc aaccctctaa accatatcaa ctatctaaac cttataaacc ctttcaacct
      241 tctaaacctt tcttaaattc attttacatt ctacattatt tagctaagac catcttttta
      301 gcggaagtat aattaccggt ggataaacga tagagataaa ttccactggc aacgcttctt
      361 cccttttcat cacagctatc ccaggaaacc tgatagtaac cgggacttgt gtgttcttga
      421 tcgtaggttt taatcttttg tcccttcata ttgtatattt caattttaac ttttccagct
      481 tctttcaggg aatagcgaat attggtattc ggattaaaag gattggggaa agcattatac
      541 aattttgtaa ccatcggaac agttggagga accggttctt gagttggatc accaataata
      601 actgttatag gaccgtaaaa ttcactgttt ccatcaaggg aaacgctttc cagccaataa
      661 taataaatca tattcgtata cacctcaaaa tccgtgtaat tatatttaat ctgagttcct
      721 tcagaagaac ctttattgat tatagaaccg ttgatcataa tagcattttg taaatcttta
      781 tcttcactac gcaaaatatt atagccggaa tgattagttt cactttctgc agtccaggaa
      841 ataataacaa aattttctga ggttactact gctgtaaaac gggataggtt taccggcaaa
      901 gtaggatcaa tgccttcttc aattactaaa ataacatcat tcttagctcc aaattccaca
      961 ttagatatat ttactatgcc ggtattccct tcggttatat tgcagggata agaacttcca
     1021 taatgccact gtccactatg gtaaattagg caataccaat ttccggaact gagttttaga
     1081 gataaattcc cttttccaga acctaataag ccaagcacaa taggagcgtt tagattatta
     1141 taattcggta atgccatcac caatggatga gtagcagtta cagcattatt aatatttagg
     1201 tggatagtgg aagttatggt tattccatct atttctgtag gataattagc tggaatatca
     1261 catgagatat gaacatcatc aagataaact gactgagaat tatttccata ataatgaaat
     1321 cccaaataga ccatttgatc cgcataatct ttcaagtcca tttgggtctt ttgccaggaa
     1381 acacagctga gattaacggg tttccagtca taattttcag gattggtatc cgttgcaata
     1441 ccccattcca aggtctcttc actattttgt ttaagatgcc agaacgaaag attatggtga
     1501 aaagcattga ttaaaacagg aggactaaac aagtattggt tataatcgtt tgaaacctca
     1561 agagaactaa atcgggctga aaattgtcca ttaaatgtgt ggttattatc attgctttga
     1621 ataatatcgt tattattatt aacatatttt gtccatccca ccggcagaaa gtcatcttca
     1681 aaatcctcca taagaagacc ctcaaagtat gtagttcctt taagaattac atcatactga
     1741 gttcgagaac tataattaat tcgcaaaata gcattttttt ctccaaagga acgaggatag
     1801 aaacgaactg gaatattaac actttgatct gtttccaaat gtgcatcaag attggaataa
     1861 tcaacctcaa aatcatcata gtcgcctaaa agcgtaaaat tatcctccgt aaggtctata
     1921 attccctctc ccgtattggc aatggtaaca tttaaatagc tggaaggagt attaacaggg
     1981 gtaaagggaa aaataatttc tgtaggtgaa taattcagaa ttggcacatc aggcagcttt
     2041 tcaatctgga aatcatcaat tccgaaactc caatttgttg ctgtggaagc atattggtaa
     2101 aaagcaagat aaatatgatg gtctctgtaa gtgctcaaat ccaagctatg ttccgtccat
     2161 tcagtattat aacaggtaaa aacagctaat tcttccgtaa aagaagagat atctgaatcg
     2221 gtagtagaaa gcaaaacttt ataggaatta ggatattggg cactttctac catatcatac
     2281 cattttattt ttatattaac cgctgttaaa tccaaaggtg gagaaataag ataatcatca
     2341 gtatttccca tcccttgagc agcatagggt tcggaatagg cgggaatatt acattcgtta
     2401 gtttgcctcc aagtataatc atcattatta gcatttatta ctatccaacc taaaggagag
     2461 ggaggactac ctgaccattc attatcaaag gtttgcaaat agggtaaatc atttcgggtt
     2521 atggttgggt cattcattac cgtaaatgac catacttcaa gctcatattt atctgaagca
     2581 tagccataaa tattataagg atcaatcttc cagtaatagg tctttccata ttccagaatc
     2641 ccgggattaa aatttgtttc attcagaagg gatataactt ttaccggatt ggatgtatct
     2701 ccaaaataaa catcatagtg agaaggacaa cctcctccac tacgccaatg cagagttata
     2761 ttttttaata catcaatagc cgaatttgcc ggaaaaggat tgatcggagg aagaggaggt
     2821 tcattttcaa ttaaaaactc cgcatcaaaa cgaattacag gcctattata agtaggagtt
     2881 aaataggtcg gttgaaagtt atcagaataa ctgccccggc agagatatgt tgatcctgta
     2941 gaagaatagg tataatcggg cctatcagtt gaccatacct caaaaccttt tacaacaaga
     3001 atatgcagat ttcccgtagc attataagga tagggatcaa aaagagtaac ctctgcccaa
     3061 cctgaagagc caaaattgtt cggaaaattg ccactgtaaa caagctgata ttcagagtag
     3121 ggtggagaga tttctccttc agataaactg gttgcggatg ataccttcat ataaatgctt
     3181 acattggaaa ttaataatga aggatttccg cggcgcttgt agaatgcaat cttattgatc
     3241 aaacactcag accgtagttc ccctgaagta tacaacattt cgtaagtgga ataattgtga
     3301 tgcctgttta tgggaattaa gccataagtt gccgaagcag gatttccaat cgtgatttgt
     3361 tctccccaac ataagaagaa aatcccgatt aaaaataata ggaacaaaaa caaaactttc
     3421 cgattcataa cttatgcctc cttaaagata ttttttcaac caaaaaagca atatttcctg
     3481 ttcccttcca gtgttgaaat tgtaagagga atcaaacacg aatacccaaa ccaatcaatt
     3541 ttaagctgga tataattgca taaattgagc ataagtagga ataaagaatt ttcactggaa
     3601 aaggaatcat ttgaagaaag aatattatga aagcgaaagg cgttatactt ggaaatagat
     3661 actactttaa catccgtctt ttttaaagtg ttttccgttg ttgctaaaaa aacgccttgc
     3721 cttaagctac tatccacatt cctctcctca aacacaaaaa aatattttat ttttattcta
     3781 tatatatttt ttcactttta ttaatcacac cttcagagct ctaacttata tccaaaactt
     3841 caatattatt ctttacttca ttctcatttt ccttttactt taaacacaaa ttcctgtcaa
     3901 gaaaaatctt acctttcccg atttccacag aaaataaagg ttttagtgaa ctcccaaccc
     3961 tgtaattgaa aacaatgagt aggcaaattc aatttttctg caaataacaa acttacttaa
     4021 gaataatttt acttcgtatt tataaaatcg caagttggta atccaatacc ttttttacca
     4081 tattcataat ctcttgaaat tatgccaaat ccagcttctt ccctactccc cactcaaata
     4141 ccactttata ttctccgata cacaacccaa gctttgtatg ggttgaggtt gagtgaatga
     4201 ttagaggatg ataagtgggt agtaaggaag cacttgtaac tatataaatt gcaatgaatt
     4261 ctataacaag gttgaaaaac attgttaggg aacttctatt cctaaaggaa ctaactttaa
     4321 gtaaggaaaa aaataatcgc taaaaagcca ctaaaagcgg ataaatagta gcaagggagc
     4381 gtaatttaac atagttattt gcttcggaaa agaaggaata ataaaatagg gatattatca
     4441 aatacgaaag caattaaaaa tgttttctgc cctctgtatt ttataataat aaaccaaaag
     4501 ttaaagaact atgaattatt ttcagaatat ggaacaagca ttgcataaaa taagagagaa
     4561 aaaaattaca gcagaaaggt aggaagctat gaaaaacata gcattattgg caatactctt
     4621 gttttcaaca gcggttctta tagctggagt aacgatgaat tactccttgg agacaccgaa
     4681 aattgaagca aaagaacaat acacgaaagt aagtttggaa aaagctcaga gttggggtca
     4741 acctggaaat ccagatttgc cctggttagg gataaaactt ttattaccgg caggttttga
     4801 agcagaaaaa attgtagtta acagatataa tcctgttact tacactttag ataaacccat
     4861 agctccgatt caaaggcaat atcccttttc ccatataata ttggaaacac cagataagcc
     4921 caatgcggaa gtttatgatt cgcctcttcc ctatcctgaa aatacggata acggattgat
     4981 tacttctttt atgtgcggac agccgatagc tttttcggca ataagtccct tttcctatta
     5041 tcctttaacc aacgaattga tattttatag acaactgagt gtagaaatta tttacagcag
     5101 cagtgctaaa gcaatggaag caatgcgttt cttaaaacag gattctttta ccgcaaaccg
     5161 tttgcaaaaa attgtggata accctgaaac catcactatt tcctccactc gtgaaacggg
     5221 aattgaatat cttattgtgg cagatgcgga aaaaataacc cagtggacac ctctaaaaac
     5281 ctttcaggaa acccgaggaa aaactgtgtt cataaaaagt atgagtgaaa ttatcgcgga
     5341 aaatcaggga attgacaccc aggataaatt gcgtaattac attatttcct tatatactac
     5401 taatcctctg cgctatgttt ttctggcagg agatacggat gttattcctc atagaggttt
     5461 ttatgttaat gtaaataaca gcgatgaagg tacggatgct gatattcctg ctgatatgta
     5521 ttatgcctgt ttggatggaa actggaatac tgacggagat agttactggg gtgaaatgta
     5581 tgaagcagac cttactccgg aactggcaat cgggcgtttt tgctataata gcgatgcaga
     5641 aatagtaaat ttcctgaata aactgactaa ttacagcaac aacccggtgg caaatgaggt
     5701 taaatcagct ttttttgttg gcgaatggtt atgggacgga cctacctggg gtggtgatta
     5761 tatggatgaa atgattggtg gaagtagtaa taatggctat actacagttg gagttcctac
     5821 ttcctggaac ataactacat tatatgatag aacttatgga gcagccgatg cctggggtgc
     5881 taatcagatt cgccctatgc ttagtcaggg tcctaatctg gtaaatcatt tgggacattc
     5941 caacactact tatgcaatgc ggttatcaaa taatcaggtt agtgcttcca caattactaa
     6001 tgacggtgta aatcataatt tttccactta ttttacccag ggctgttatg ccggtgcttt
     6061 tgataatcgt gaaacttctc ccgggcagta tacatccgat tgtataacag aaaaaatgac
     6121 ctctattgcc acaggacctg tagcaatgat ttctcattca cgctatggct ggggtgttca
     6181 acaaagtact gatggagctt ctcaatatat tcatcgccag tttatagatg ctatttttgg
     6241 ggaaaacatt tatgaagcgg gttttgcctt ggttgattcc aaaatagata atattcccta
     6301 tatcactaat gaaccggtaa tgtattgggt aacttatgaa accaatcttt ttggtgatcc
     6361 tgctatgatg gtttggacgg acactccaca aactataaca gctaatttac cggaagtatt
     6421 gatggtagga caaaatcatt atcagattca gaccaatgca ccttatgcgg tatttattct
     6481 gaaaaatgaa gatcataccg tatacaggac ttccgcagat gaaaatggtt tgattatagt
     6541 taattttttc gctccgctaa ctccaggaac ttattacatt tatatcagtg cccacaattt
     6601 ttatcctttc aatgctacca ttacagctac agcggctcaa atgccctata ttatatgtaa
     6661 taatgtcagt tataacgatc aagatggact ctatcataca ggtgaaacat tatcccttaa
     6721 tctctttgtt aaaaatgtgg gcttgatgga tctcactgtt cccgggactc taactttcct
     6781 cagtaattcg gctaatatcc aaattctttc ctcaacggca aattttaccg gtattgctgc
     6841 tggtgattct ttagctctta attctgtctt tttgttcagg attgtaggca attttactga
     6901 tcaaacccaa gccaccttca caatcagagc caattatgat tcctacttat ccaattctac
     6961 ctttatctta actctgaatg ctccggtttt acaattgctt tcttatcagc tgaataatat
     7021 ggatgttatg attatgcctg gagatacacc ttccattagt ttcagcgttg ctaataccgg
     7081 tagtggaaat gcttatacac ctatgttaat tatttttaac tcctctcccg atattatttt
     7141 ggagcaggat gaaattttgc tggctccctt aagtcctcat agcacagccg actaccaaag
     7201 tgcaatcact ttacaggtat tggatagtgc ggttattgat aataactata tgataaatta
     7261 cattttcagt gctgaaaacg gaaatctggt agaaggaaat ttcttaattc atattggtgc
     7321 tctcagttac agttttgaaa ccgattacca aacatgggaa accgttgctc tcagcagtaa
     7381 ttttgtaaat cagtggcatc gttcatcaaa tcgtaatcat actaccaacg gtatttattc
     7441 aatgaaattt ggaggtaacg gttcttctaa ttatgccagt agtgcttatg gtgcacttat
     7501 tagtcccgta atgaatgtta gccccaattg tcaattgaaa ttctatcact ggatgagcgc
     7561 agaaaaacat agtaccaatc aatcctacgc ttgggatggt ggtttggtgc aaatgagttt
     7621 gaacggaggc aattggactc aaattacccc tgtaggcggt tatccttata aaatttacaa
     7681 taattccgct tcaccttttt ccgcaaatac ttgggtctat tcaggtaatt ttgattggac
     7741 ggaagctatt tttgaacttg gtaatgtttc cggaatagct caatttcgtt gggtttttgg
     7801 cagtgatggt tatgttggtg gagagggatg gtatatagat gatgtgagga taacgggcta
     7861 tgttgccaat gaggatgaaa gcattccttt agcacaaaat gttatcctgt atgataatta
     7921 tcccaatcct tttaatcctg aaactacgat tgagttttcc ataccagctc ggatgccggt
     7981 ttgtctggag gtcttcaata ttaaaggtca attggtaacc cgcttaattg atgaggtttt
     8041 acctgcaggc actcataaag tcgtattcaa tggtttagat ataaacaagc gtaatatagc
     8101 cagtggagta tattattatc gcattaaaac tacttcaggt gtccaaatgc gaaaaatgct
     8161 attactgaaa tagaaatgct aaaatttact cccgatacaa atgttctcgt tttaaccgga
     8221 gccggaatca gcgctgaatc cggtatccgg acttttagag atagcggggg tttatgggaa
     8281 aatcatcggg ttgaagatgt tgctactcct gaagctttta actctaatcc ggttaaagtt
     8341 tgggaatttt accgtcaacg ctaccaggat agtatttcca gtaaacccaa tccggctcac
     8401 tttgctttgg ttaaattgga acaattttta ggggataact ttttactggt tacccaaaat
     8461 gtggattgct tgcatataaa agccggctct aagagagttt tggaaatgca tggctccctt
     8521 gccaattgta tctgcaccaa atgccatagc aaattagcaa ttaaggatat tgatttaact
     8581 caaccgattc ccctttgtcc cttttgcaat gggtttttaa gaccagatat tgtctggttt
     8641 ggtgaaatac cgcactatct gagtataatt gatgatgctc tcagaaaatg ttctgtattt
     8701 atcattgtag gaaccagcgg agttgtttat cctgcagcag gtttcgttct tactgccaaa
     8761 ctgatgggag caaaaacaat tgcaataaat ctggaaaaac cggataacct tgcctatatt
     8821 gatgaatttt atcagggcaa atcaggagaa attctgcccg gattagtagc tatgtggatt
     8881 ggcgaatagg attattgctc acagattaca cagatttcag agataaaaaa ttgataatta
     8941 agcaggggta ggaaatgaag aatgaaaaag tgagattaaa aaaagtgagg gctaaagcag
     9001 tgagattact tccggaataa ataatttttc cgttctcact ttttttacat ttttcttgtg
     9061 taataacgac tcttccagat tacgcttata ttgtaaaact aatttctttt ctctgtgttt
     9121 tctttttcaa tttcattttt ttcgttgacg ctaaccctga acaaataaag atggtagttg
     9181 aatcgctttt atcaaataag gagttgtaaa taatgcgtaa gttctttata atgataataa
     9241 tgctctttgt ccttttagcc ggctgtaaaa gtgctttaca gtccaataag gttaaagttg
     9301 ctttctggca tggtctcagc ggtcctttag gtgatactct taatgaaatg attcgtgaat
     9361 ttaatcacac tcataaagac atagaagtaa ttgccaatcc tattagtagc tatacggcgc
     9421 tctcgcaaaa acttatggct tccattcaag caaaaaaaca gcccgatatt gctcaggttt
     9481 ttgaatcttg gacttccaaa tatatagagg caggagtgct ttgcaatttt gaggatttaa
     9541 tgaaagcgga ttcaacctgg acaatgcaaa gtttggaaga tatttatcct gtcttcttaa
     9601 aatccaatac ttttaatgac accatctatt cctttccctt caataaaagt gtccgtgctt
     9661 atttctataa taaagatgct ttctatagag ccggtcttga ccctaatcat tttcctgcta
     9721 cttgggatga atttcggtcc tactgtcgta aattaactac tgataaaaac aatgacggta
     9781 aaccagaaac ctacggaacc aattttaatg tgaatgaatg gcaatttatt aatctgcttt
     9841 accaagctgg gggaacaatt ttagatgaaa aaggaaaccc acaacttaat agtccacaag
     9901 caattgaagc tttaactttt actaccgatt tgctaaataa agataaaacc gtttatcttg
     9961 tccgtgaatt tgaaggacaa aatgattttt tagccggaat tgccgctatg tatgaaggta
    10021 gcagtgtttc tatcacgcat atgcgtcagc aaccaatcaa ttttaatatt ggttatgctc
    10081 ctttacctgt atatagaacc aataaaagtg ccgtcagtgg aactaatatt gttattttta
    10141 aaagcggtga tagaaaaaga gaacaagctg cctgggaatt tataaaatgg tttacagata
    10201 cagaacaaac agccagatgg agtgctgaaa cctgttatat gcctgtgcgg aaaagtgcta
    10261 tgcaatctcc tgttctgcag aactttttaa aagaatatcc tcaatttcaa ggtatttatg
    10321 atcagctgga aacagctatc tttgaacccc aaatttctgc ctggttcaaa gcccgtatgg
    10381 aattaaaaaa ttatttagag aaagcaatgc ggggaatttc tactccccaa gaagcattgg
    10441 atgaaatgaa cgcaaagttt caagccatta ttgaggaaga aaaatatcta aaaagcttgt
    10501 aactattaac caacgaggaa aatatgattc ttaactgcgc tgaagaacta atcctgctat
    10561 ccatagacga tcaaactaat tcattttaca ggataaccaa catcaatttc aatgtggctt
    10621 taattggtgc tcttttaatg gacttggctt tgagaaaacg aattgatgta gatctggaag
    10681 gtatctatgt tctatctaca gaaccgacag gtgataagtt tctggatgct atcctggaaa
    10741 atttaattga aactgaagca ggaaaccaac cggctgtttt agtaggacag ctttataact
    10801 ctatgggaca cttgaaagat aaactcctgc aggacttgga agaaaaaggc atcatcaagg
    10861 tgagccagtg taaagtcctt tggctgttca aaaagcgtcg ctatcccatt atagaccaga
    10921 aagaagagga agaagtcctt tcccgaattc ggaaaacggt tcttacagat gttgatcctg
    10981 aaccccgaga cctggctttg attgccttac ttaatatttg caatctgatg gacaaaatct
    11041 ttactcaaga ggaattggaa caacgcagtg aacgcctgga acagttaaag aaactggacc
    11101 tcatcagccc tgccgtaaaa agaattattg gcgaaatgca ggttttaatc acaactactt
    11161 acacaacctg aaaactttag gattattcaa aaacggattg caggataaac gcaaataatg
    11221 cggttaaaaa attgactggc aaattctatc tttgccaaag aaaccaaatg tttctaaagg
    11281 aatttatacc ttcggcattt gccgaattgg aatttggcaa cccaattgat tattatgttt
    11341 atacttattt atgctgcatt atttaggata aaggactgca tcctttgtgg caaaacggaa
    11401 tttgccacct cattattctt ttccttgact tcatagaccc catccaaatt tttgagctca
    11461 ataaaaaagc gggcatagct caggtggtag agcattagct tcccaagctg agggtcgcgg
    11521 gttcaagtcc cgttgcccgc tccattttat gtagccagaa ctcttctttt ttttgtgcag
    11581 caggagctcg ctgaagctct gcatcttttt ttacaatgtc cactccctgt ttcacctttc
    11641 cacttttcca ccttttcaca ctcttgcaga ggaacagcgt ttttcggcta cacaaaaaag
    11701 cggaggaaca gtatcctccg gctacacaac attaaggatg ctgagtactt gccttaatgt
    11761 aataaaatcc ctgattacct gatgcatcag tgaattgaag gtaggaagta gttccaatta
    11821 aggtaaaatt gctgtaagga ttatcactgc gataaatttg atagctaaga gcattcggaa
    11881 cagcattcca gcgaagaact atgttattat attctcggga aatagttaca ataggtgttt
    11941 ccagagaagg ataataaccg ctaccattta agaccaaatt gaagttatga ctatttacag
    12001 cattgctggt tataaccaaa tttccattat atgaggtttg agctgtgggt gtaaaagtaa
    12061 cattaacggt caatgagttt cctgcctcta tagcaaattg catatttgtg cgatcgctat
    12121 atctggtttc ctgtaaatct ttatttttat tccttggggc aaaatcaaga gtatagttat
    12181 tcggaacggt aaaatagcca gccagaagtt gattgcctgt attttgaatg gtaaaaggca
    12241 aagttgcaga attacctatt tctattgccc cgaaattcaa ttgggaagag cttatagaaa
    12301 ttatcgcatt ttgtggttct gaaggaactc ttattttaat atttggtcta ttgcttgatg
    12361 ttgagctacc tgtaaaaaca ttggtttggt cactgctatc actccaagta taacggtagc
    12421 cattgttaac agaagtgtat tgaactgtcc cggatgaatt ccattgactt acaagtccaa
    12481 aagcagtatc tattacaatg ttatcaattc cattccagtg aaaaggtgaa gtgagagtta
    12541 gcatatccca acctccagca actggcatat aggatgcatt ggtataaacc gtagtcattc
    12601 cagttgctgt ctgccaggaa gaaacatcaa tagcagttgt atgtttcatt cggattaaaa
    12661 aattcggtaa agcataagct ggagagctgg ctatataaaa acccagctgt aaaatatcta
    12721 tcggtccata aatccctgca gcatttagct ctgcagcagt atataccgat tgaccgtgca
    12781 aacttttata atagatattt acaggactgg ctgtattagt gcccgttata cttgttccag
    12841 tacctattat agtttcggaa actgaagaac tggaaggagt tgcttgcacg gttaaggatg
    12901 gaagcgattc attaattccg gaaacagagg tcagataata ggaataggtt gtgccattaa
    12961 caacatctac atcataataa gtatatctat tagctacatt atcatataag gcattattac
    13021 ggtatatatt ataactatct atatttccag ccataggtga ttgccaggta agagttacta
    13081 ttccattgcc ggaagtagct cttaagg
//
LOCUS       DAOPFL010000042        13105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5561_length_13105_cov_94.624215, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000042 DAOPFL010000000
VERSION     DAOPFL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5561_length_13105_cov_94.624215"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLG69_04370"
     CDS             <1..290
                     /locus_tag="PLG69_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42778.1"
                     /translation="PDCTLEGAILSFSENVYSYDAANQVQDYQVKMVVSVTFTDLINN
                     TVLYQNSGLTVTELYAVAEGGTAKFKTKEEAVEELISKLYKTILQNSIEGW"
     gene            229..1017
                     /locus_tag="PLG69_04375"
     CDS             229..1017
                     /locus_tag="PLG69_04375"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPI42779.1"
                     /translation="MKSSSPNSIKPSSKTVLRAGKTLRLNRYLADCGLGSRRKVEEFI
                     TTGRIAVNGNLVQDLSRQIIPGKDKVTLDGKLIQPAKEKFYLILNKPRGYVVSQNDEL
                     GRKTVYSLLPPKGKQLAYAGRLDKESEGLLFFTNDTALINLLTHPKNKVEKVYRVDIN
                     RPLNNMELNMLRKGVEIEGGKTLPAGIFVKSKNANGMTLKIVITEGRKRQIRQMIEAV
                     GAKVTYLKRLQFGPLKLKDLPVGRWRLLEPGEVKALLSLKKEKT"
     gene            1018..2094
                     /gene="ychF"
                     /locus_tag="PLG69_04380"
     CDS             1018..2094
                     /gene="ychF"
                     /locus_tag="PLG69_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPI42780.1"
                     /translation="MKAGIIGLPKTGKTTIFNALTRGEQSTDKYAPSSLEANIGVVQV
                     ADKRIDKLSELYKPKKTIYATIEFHDYPGIFYKESEISENALLAEIKNAEAFILVLRG
                     FRDEELEGLFPLGTPIEQFQHFQEEMLLYDLIMAEKRLEKIELGYKRGVKTPAVQIEE
                     KALLKIRDFLQNNNPLINLELNPEEEKAIRGFQFFSAKPMLILINCNEDNINAWDETL
                     KTFTEKGYYAYTIAGKFEEELSKLENEEAQMFMEDMGISESIRDRLTHWTYQLMGYIS
                     FFTVGPDEVRAWTIEKGANAVTAAGKIHSDLARGFIRAECFQYDDLITYGSEKALKEK
                     GLFRLEGKDYIVKDGDILSIRFNV"
     gene            2116..4365
                     /locus_tag="PLG69_04385"
     CDS             2116..4365
                     /locus_tag="PLG69_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPI42781.1"
                     /translation="MANKPQRKVNNRKTNKKGNPLTKRILSGIICLLSCLIILSLTVG
                     YISIKDDLETLKASGNIISWFKEGNSTTNNPIGIFGIVCGYIFIYLFGYWFSLIGFII
                     IAIVALHYFLTPESLKHRQKGYLLLIIMFFLQALIVSKQENYIHSVVPLFVYRILAAV
                     FSGIGANIILIGAMILTAIMLIEYERLKSWILVARENLQRKKEQRTKTVQPTITANNL
                     HPVPKAAEETIPVIQNHNQPNPPPEPFFEDIIKIPKEYKPKPVATEEDADREYQLPSI
                     DDFLESPIKLSERERKEIENQIITTSQVLKSKLAEFGIEAEVKNVNIGPIITQYELEP
                     AKGIKVSRFTSLADDLALAIKAKSIRVQAPIPGRGLIGIEIPNLARDMIYLKDLLLSE
                     QMRQTTSKLAFGLGKDIAGKPIVADLAKMPHLLIAGATGSGKSVCINTIIMSLIMRTK
                     PDELRLILIDPKRVELAGYNELPHLIGQVVTDADTALETFIWAVREMERRYEVLQEAK
                     VRDIIGYNEKCREDEDLEPLPYIIIIVDEFADLIMTSGKDIEMPITRLAQMSRAVGMH
                     LILATQRPSIKVITGIIKANFPARIAFQVSSRVDSRVILDMIGAERLLGNGDMLFLPP
                     GKALPERIHGAFVSDAEIARVCNFLATQPKPKQDFSLVIEKNEGIGFFDYDDELFPEA
                     AKVVVSTGTASVSMLQRHFKIGYARAGRLIDLLERARIIGPHLGSKSRDVLATREDLI
                     RIGVIDEDR"
     gene            4352..4972
                     /locus_tag="PLG69_04390"
     CDS             4352..4972
                     /locus_tag="PLG69_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPI42782.1"
                     /translation="MKTAKIITLILLLCCNFLFGISSEALYQKIAKTYGSLSSFQAVI
                     KQDNYFAQLDKSITYHGNIYFTKGRMVIRFNKPYFQRLSVSGGIVELYDSQSNTVFRS
                     KMRPEFGKMNPVEILQLYWKKSTVNIQQGKGNLVNVFLKPFDDPVIVSISAVVNSESG
                     IVQSLTWTNRNNDKVTYNFSSIKTNAKIPASVWQYTYPKDVQVVEQ"
     gene            4969..6024
                     /locus_tag="PLG69_04395"
     CDS             4969..6024
                     /locus_tag="PLG69_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPI42783.1"
                     /translation="MIALIMAGGVGTRFWPLSRKNLPKQFLRLFGDLSLLQMTVERLL
                     PRFSLADIYIVTASSQVELIKQQLPNLPQQNIIIEPFGMNTAPCIALSIEYLKNIYGQ
                     KETMLVLPADHLIRDQEKFLESLNPAEREAEKGCLITFGIVPVYPATGYGYIEAGEEI
                     APGVRKVLHFKEKPDKQTAEEFLAKGTYFWNSGMFCWSLESISTAFETLLPDVYGLTN
                     EIGKIWKNEGLDKDIASIYAQMPRIPIDIGIMEKANFRGVIPVSYDWSDVGSWKSLAD
                     LTAGDADNNYFSSSGKAISAKNNYVVSDKFTALIGVNDLCLITTKDAILLCNKEMAEE
                     VKKIVDYLEQQNRQDLL"
     gene            6043..6939
                     /locus_tag="PLG69_04400"
     CDS             6043..6939
                     /locus_tag="PLG69_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPI42784.1"
                     /translation="MDMKTFGYLYYKTRGYSREDIIKQAGISEEDFEFLEKNLQDVLK
                     EIEMLRPVAENIGQDFTRLTRYIYERESDQTLGVPRPDAIKVRSGEIVPLPAIGTLKM
                     PDMPLAKAIEQRRSVRKYSAIPLQQEELSFLLWASSWARDFRSSEQMEITFRNVPSAG
                     SRHPLETFLDIRRVEGIKPGLYYYHPVKHCLVLYDDSSEIAAKIHEGCMFQEMIPKAA
                     VNFILTAVPYRTVWRYGQRGYRYLYLDAGHIGQNIHLAAEAIDAGACMIGAFLDEAMN
                     EALGLDGIEEFVIYIASVGKKP"
     gene            complement(7452..8159)
                     /locus_tag="PLG69_04405"
     CDS             complement(7452..8159)
                     /locus_tag="PLG69_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPI42785.1"
                     /translation="MKIPTVEDLINIYDAIIKGIDKEALNSKDRAYGGIIRAGKGSLV
                     ENIAKGMIQIAWDRLNGDQTHLSLNKTKVRIPIKADYVKRIKNPEIKSYIQDHLGNYY
                     YELNIDVPVYIDNELVLGVECKSYTENAMLKRILVDFTLLKTQYPTLDCVLLQLESQL
                     NGDYSNISQSIIFGSSSTHTLLSYFDVDLYIITLLQGERKVKKPIHKKEYFKELTSES
                     IEKAISTFQNILSKHKK"
     gene            complement(8258..9469)
                     /locus_tag="PLG69_04410"
     CDS             complement(8258..9469)
                     /locus_tag="PLG69_04410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPI42786.1"
                     /translation="MSIYSDKKLKRNDNIKKKKVEVRMVNYKEIKVEFDYPINNYTMS
                     ILNALEKKYGESNIHFDYNDNQVLVYLNNIDFEMFKSDIEELSLNNNNNDLNKISSAI
                     SNIKSYGIKGKKRLYVGYGDERKTVNRKEKEKKREIFYYTRGNDFSEINNLLPDKYMD
                     RIVCGDSKIILSQLPNNCIDLIFTSPPYNFGLDYEENEDDQHWEKYFETLFAIFDECI
                     RVLKYGGRFIINVQPLFSDYIPTHHIISNYLMQKKLIWKGEILWEKNNYNCKYTAWGS
                     WKSPSNPYLKYTWEFIEIFCKGTLKKIGDDKKIDISGDEFKKWVYAKWSIAPERRMKE
                     FNHPAMFPEELATKVIKLFSYQEDIVLDPFNGVGTTTLVAKLNKRHYLGIDISSEYCS
                     KAEERLSSFLF"
     gene            9987..10646
                     /locus_tag="PLG69_04415"
     CDS             9987..10646
                     /locus_tag="PLG69_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI42787.1"
                     /translation="MSEILSIDIKRLFYGAQVLLNEIQLKVEKGERILITGITGSGKS
                     SLLSTLNLMNRNYEGEILFEGKNIVEYQPDILRSKICMVLQEPYLEDANVQEVLDLPL
                     HYSSLKHRRLPDRQERIKNLFTAFQLSYEYLQKPTSQLSVGEKQRIALIRVLQLQPDI
                     LLLDEITSALDQKTSGIISECIFNNFAGTVIAISHDPLWQNLWQRNWVLEYGTIIDRR
                     I"
     gene            10651..11415
                     /gene="fetB"
                     /locus_tag="PLG69_04420"
     CDS             10651..11415
                     /gene="fetB"
                     /locus_tag="PLG69_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HPI42788.1"
                     /translation="MDISIYRLILAVLLLIFPLLIFSNLKLKLSGQLFNSFARMIVQL
                     AIIGLILQFIFNRENPWLAFLWMLIMLANAVLTLKGRLKFQKKILLPVLIFSLLTTTL
                     IVMPWLIIVVLRPEPLFAPRFLIPIYGMILGNSMNNCSLALERFESGLSENWKAYYTR
                     LSLGASQWEAILPAFRKAMQAALMPELLTIASMGLVTLPGMMTGQILGGASPLVAIKY
                     QMMIMIGIFSGVTITDYTAINIYLRKRFDKFYLPKP"
     gene            11728..12432
                     /locus_tag="PLG69_04425"
     CDS             11728..12432
                     /locus_tag="PLG69_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HPI42789.1"
                     /translation="MDYDVYCDESYPDLFSSKKPKAKYLVIGSLWLCKQNRNVFKKEI
                     HSLRDKHKMGRGEFKWKKVSKSRLAFYEELLDWFIAKGDELSFRCIIVDNAKINLVEF
                     HKDDQELGFYKFYYQMLHHWLEDNNSYNIFCDNKTISTKSRLITLRECLRNANKSSWV
                     TDIQAVTSKESVLLQLSDVLVGLASSKFNNNLKQGSLKSELISYFEQTMGREIMPTSK
                     DEKKFNVFKIKLRDGR"
     gene            12433..12714
                     /locus_tag="PLG69_04430"
     CDS             12433..12714
                     /locus_tag="PLG69_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42790.1"
                     /translation="MNYPPFLNLNSIDEYRAHFEKTYCRCPLQTFDKYIVRFKKSDFE
                     HAFYESSRRNGIKDNFSKNRALRMDWIKYALMDSTSILKVGWDKNKKNI"
BASE COUNT         4347 a         2201 c         2382 g         4175 t
ORIGIN      
        1 accccgattg cactctggaa ggagctattt taagtttttc cgaaaatgtt tacagttacg
       61 atgcagctaa tcaagttcag gattatcagg taaaaatggt cgtttctgtt acctttaccg
      121 atttaatcaa taatacagta ctttaccaaa acagcggttt gactgtaacg gaactttatg
      181 cagttgccga aggaggcact gccaaattta aaactaaaga ggaagcagtt gaagagctca
      241 tctccaaact ctataaaacc atcctccaaa acagtattga gggctggtaa aacccttcgt
      301 ctgaaccgtt atttagctga ttgcggttta ggcagcagaa gaaaagtaga agaattcata
      361 actactggta gaattgcagt aaacggtaat ttagtgcaag acctttcccg acagattatt
      421 cccggcaaag ataaagttac tttagatgga aaactgattc aacccgccaa agaaaagttt
      481 tacctaattc ttaataaacc gcgtggttat gttgtttccc aaaatgatga gctgggaaga
      541 aaaacggttt attctctttt accgccaaaa ggaaaacaac ttgcttatgc tggacgcctg
      601 gataaagaaa gtgaagggct gttgtttttt actaatgaca cggctttaat aaatctatta
      661 actcatccca aaaacaaagt ggaaaaggtt tatcgcgtag atatcaaccg tcccctgaat
      721 aatatggaac tgaatatgtt gcgtaaaggt gtggaaattg aagggggaaa aactttgcct
      781 gcaggtattt ttgtaaagtc caaaaacgcc aacgggatga ccttaaaaat agttattacc
      841 gaaggcagaa aaagacaaat ccgccaaatg atagaagcag ttggagcaaa ggttacatat
      901 ttaaaacgct tgcagttcgg tcccttaaaa ctgaaagacc tacctgttgg cagatggcgt
      961 ctattagagc ccggtgaagt gaaagcactg ttatctttaa agaaggaaaa aacatagatg
     1021 aaagcaggaa ttatcggtct gcctaaaaca ggcaaaacaa ctattttcaa tgcattaacc
     1081 agaggtgaac agagcacgga taaatatgct ccatcctctt tggaagccaa tattggtgtt
     1141 gtgcaagttg cagataagcg tattgataag ctttcggagc tttacaaacc taaaaagaca
     1201 atatatgcca ccattgaatt tcatgattat cccggtattt tttataaaga gagcgagata
     1261 tctgaaaatg ctttgcttgc agagattaaa aatgcagagg catttatctt agttctgcgt
     1321 ggctttaggg atgaagaact ggagggtctt tttcctttag gcaccccgat agaacaattt
     1381 caacactttc aggaagagat gttactatat gatttgatta tggcagaaaa gcgtctggaa
     1441 aaaattgaac tgggttataa aagaggagtt aaaaccccag ctgtgcaaat tgaagaaaag
     1501 gcattactca aaatcaggga ctttctccag aataataacc ccttaataaa tttggaactt
     1561 aacccggaag aagaaaaggc aatacgcggt tttcaattct tttctgccaa gccaatgttg
     1621 attttgatta actgcaacga ggataatata aatgcctggg atgagacctt aaaaacattt
     1681 accgaaaagg gttattatgc ctataccatt gccggaaagt ttgaagaaga gcttagcaaa
     1741 ctggaaaatg aagaagcaca aatgtttatg gaagatatgg ggattagtga atccattcgt
     1801 gatcgcttaa cacattggac ttatcagctg atgggttata tcagtttttt cactgttggt
     1861 cccgatgaag ttcgtgcctg gacaatagaa aaaggtgcta atgccgtaac tgctgcaggg
     1921 aaaattcata gtgacctggc gcgtggtttt attcgtgcag agtgttttca gtatgatgat
     1981 cttataactt atggcagtga aaaggcgctt aaagaaaaag gacttttccg cttggaagga
     2041 aaggattata ttgttaagga tggagatatt ctttccatcc gttttaatgt ataaaaaatc
     2101 tgggagcaga taacagtggc aaataaaccc caacgaaaag taaataaccg aaagacaaat
     2161 aaaaaaggaa atcctttaac taaaaggatt cttagcggta ttatctgttt gctgtcctgt
     2221 ttaattattt tatctcttac agtgggttat atttccatta aagatgatct ggaaacccta
     2281 aaagcaagcg gtaatataat ttcctggttt aaggaaggta attccacaac gaataatccg
     2341 ataggtattt ttggcattgt ttgcggttat atttttattt atctttttgg ttattggttt
     2401 tctttaatcg gttttattat tatagctatt gtagcgttgc attatttttt aactccggaa
     2461 tcattaaaac ataggcagaa aggttatctg ttattgataa ttatgttttt cctgcaggca
     2521 ttgattgtat ccaagcaaga aaactatata cacagtgttg tgcctttatt tgtttaccga
     2581 attttagctg ctgttttttc cggcatcgga gccaatatta tcctgattgg agctatgatt
     2641 ttaacggcaa ttatgctgat tgaatatgaa cgactaaaaa gctggatttt agttgccagg
     2701 gaaaacctgc aacgcaaaaa ggaacaaaga acgaaaactg tccagccaac tattactgcc
     2761 aataatcttc atcccgttcc aaaagcagca gaggaaacga ttcccgttat tcaaaatcac
     2821 aatcagccga atcctcctcc cgaacctttt tttgaagata ttataaaaat ccctaaagag
     2881 tataaaccca agccggttgc aacggaagaa gatgcagacc gggaatatca gttaccttcc
     2941 atagatgact ttttggaaag cccgattaaa ctttccgaac gcgaacgcaa agaaattgaa
     3001 aatcaaatta tcactaccag tcaagtatta aagagcaaac tggcagaatt tggtattgaa
     3061 gcggaagtta agaatgtaaa tatcggtcct ataattaccc aatatgaact ggaaccagct
     3121 aaaggcatca aagttagccg ttttacttct ttggcagacg atttggcttt ggctattaag
     3181 gcaaaatcaa tcagagtgca ggcacctatt ccgggacgag gtttaatagg aattgaaatt
     3241 cccaatctgg caagagatat gatttatttg aaagacctct tgctttcgga acagatgcgg
     3301 caaacaacat caaaattagc tttcggttta ggtaaagata ttgccggaaa accgattgta
     3361 gccgatttgg caaaaatgcc tcatctttta attgcaggag ctacgggaag cgggaaaagc
     3421 gtttgtatca ataccataat aatgagttta attatgcgca ccaaacctga tgaactgcgc
     3481 cttattttaa ttgatcccaa gcgggttgaa ctggcaggtt acaatgaact tcctcattta
     3541 atcggacagg ttgtaaccga tgcggacact gctttggaga cctttatttg ggcagtgcgc
     3601 gaaatggaaa ggcgttatga agttttgcag gaagctaaag tgcgagatat tattggctac
     3661 aatgaaaaat gccgggaaga tgaagacctt gaacccctgc cttatataat tatcatagtg
     3721 gatgaatttg ccgatttaat tatgaccagc ggcaaggata ttgaaatgcc tataacgcgt
     3781 ttagcccaaa tgtcaagagc tgtaggaatg catttaattt tagcaactca gcgaccatcc
     3841 attaaagtta taacaggaat tatcaaagcc aattttcctg cccggattgc ttttcaggta
     3901 tcttcccgag tagattcccg cgttatttta gatatgattg gagctgagcg acttttagga
     3961 aatggagata tgctgttttt acccccggga aaagcattac cggaacgcat tcatggtgct
     4021 tttgtttccg atgcggaaat tgccagggtg tgtaactttt tagctacaca gcccaaaccc
     4081 aaacaggatt tttcgcttgt tattgaaaag aatgaaggta tcggcttttt tgattacgac
     4141 gatgaattat ttcccgaagc agcaaaagtg gttgtttcca ctggaactgc ttccgtttct
     4201 atgttgcaaa gacattttaa aattggctat gccagagcgg gacgcttaat tgacctttta
     4261 gaaagagcca gaattatagg tcctcatttg ggcagtaagt cccgcgatgt tttagctacc
     4321 cgagaggact taattcgcat aggagtgata gatgaagacc gctaaaataa ttacccttat
     4381 ccttttactg tgctgtaatt ttctttttgg tatcagcagt gaagcattat atcaaaaaat
     4441 tgccaagacc tacggaagtt taagcagttt tcaggcagtt ataaaacagg ataactattt
     4501 tgctcagctt gataaaagca tcacctacca cggaaatatc tattttacca aaggaagaat
     4561 ggttattcgc tttaataaac cttattttca acggctaagt gtttctggtg gaattgttga
     4621 actttatgat tctcaaagca ataccgtatt ccgcagcaag atgcgtcctg aatttggaaa
     4681 aatgaatccg gtggaaattc tacaactata ttggaaaaag tccactgtca acattcagca
     4741 gggtaaagga aacctggtga atgttttctt aaaacctttt gacgacccgg ttatcgtatc
     4801 tataagcgct gtagtaaatt cggaaagcgg aatagtgcaa tctttaacct ggactaatcg
     4861 taataacgat aaagttacct ataatttttc cagtatcaaa accaatgcca aaattcctgc
     4921 ctccgtttgg caatatacct atccaaaaga tgtgcaggta gttgaacaat gattgctctt
     4981 attatggcag gaggagtggg aacccgtttt tggcccctca gcagaaaaaa tcttcctaaa
     5041 caattcttac gcctttttgg tgacctttct cttttacaaa tgacggttga gcgtctttta
     5101 ccccgttttt ctcttgccga tatttatatt gtaacagcga gtagtcaggt agagttaatt
     5161 aaacaacagc taccaaatct tccccagcaa aatatcatta ttgaaccttt tggaatgaac
     5221 acagcaccct gcatagctct tagcatagaa tatctgaaaa atatttacgg gcagaaagaa
     5281 acaatgctcg ttttgcctgc agaccatctt attcgggatc aagaaaagtt tttggaatcg
     5341 ttaaatcccg cggaaagaga agcagaaaaa gggtgtttga ttacttttgg tattgttccg
     5401 gtttatcctg ccacgggata tggctatatt gaagcgggag aagaaattgc acccggggtt
     5461 aggaaagttc tccattttaa ggaaaagccc gataagcaga ccgcagaaga atttttggca
     5521 aaaggaactt atttttggaa cagcggtatg ttttgctggt cactggaaag tatttctacg
     5581 gcgtttgaaa ccctattgcc cgatgtttat ggcttaacta acgaaattgg taagatatgg
     5641 aaaaatgaag gtttggataa agatattgcc tctatttatg cccaaatgcc acgtattcct
     5701 attgatattg gtataatgga aaaggcaaat ttccggggag ttattcctgt ttcctacgat
     5761 tggagcgatg ttggaagctg gaaatcgctt gccgatttaa cggcaggaga cgcggataat
     5821 aactatttct cttcatcagg aaaggcaatt tccgctaaaa ataactatgt tgtttccgat
     5881 aaattcactg ctttaattgg cgtaaacgac ctttgtttga ttacaaccaa ggatgctatt
     5941 ttgctttgta acaaagagat ggcggaagaa gtaaagaaaa ttgtggacta tttggaacaa
     6001 caaaatcggc aagacctgct gtaagaaaag gagaaacaaa aaatggatat gaaaaccttt
     6061 ggctatttgt attacaaaac ccggggctat tcccgggaag atattattaa acaagcagga
     6121 atcagtgaag aggattttga atttttggag aaaaatctgc aggatgtcct caaagaaatt
     6181 gaaatgttaa gaccggttgc cgaaaatata ggacaggatt ttacccgcct aacccgttac
     6241 atttatgaac gcgaaagtga ccaaacatta ggagttcccc gtccggatgc cataaaagtt
     6301 cgcagcggag aaattgtccc tttgccagca ataggaacct taaaaatgcc agatatgcct
     6361 ttagccaaag ccattgaaca gcgtagaagt gtgcgtaaat atagcgctat tcctctgcaa
     6421 caggaagaac tctctttttt gctgtgggct tcttcctggg caagagattt tcgttcttca
     6481 gaacaaatgg aaatcacttt tcgcaatgtt ccttctgccg gctcgcgaca tcctttggag
     6541 acatttttag acatccgcag agtggaaggt atcaaacccg gcttatatta ctatcatcct
     6601 gtaaaacatt gtctggtttt atacgatgat tcctctgaaa ttgccgctaa aattcacgag
     6661 ggatgtatgt ttcaggaaat gattcccaag gcggcagtga acttcatttt aacggctgtt
     6721 ccctatagaa ccgtctggag atatggacaa cgaggatatc gttatctcta tctggacgca
     6781 ggacatatcg gacaaaatat ccatcttgca gcagaagcca ttgatgccgg tgcctgtatg
     6841 attggcgctt ttttggatga agcaatgaat gaggcacttg gtttggacgg cattgaagaa
     6901 tttgttatct atattgccag cgtagggaaa aaaccgtaac ctgtttttcc taaaactttc
     6961 atattattaa tgcaattata acaataattt tattagttct aacctgaacc taaaaaacaa
     7021 gagaaccatt actgccaagt ttgatgtaac caactaaaat ataagtatat taaccccatt
     7081 atttgtccgt ttcagcttaa agtgtataat tgattgttac atagttaatt attagacgac
     7141 tccctaacac taccgtaaca cttccgtaat ctcgttacgg agttgttact tgattgttac
     7201 atcgtcatta ccgggttatg taaacaaaaa ataattggag catttccttt tttcatatat
     7261 cattttttga atggaaatcc ttttttcatt tctgcgtaat caggatttgc ccaccatttt
     7321 taccccaatt tcttattatg atattttttg cctgaaaagc tatttatcat tcttgtgaga
     7381 caatgaatcc aaataaactt aaaaatatta acttcatttt caatatgaaa attgcattat
     7441 gatttatcca actacttttt atgcttacta agaatatttt ggaaggtaga aatggctttt
     7501 tctatagatt cagaagtaag ttccttaaag tattcttttt tatgaatagg tttttttact
     7561 ttcctttcac cctgaagtaa ggtgataata tataaatcaa catcaaaata ggataataga
     7621 gtatgagtgg aactactacc aaagataatt gactgactga tatttgaata atccccattt
     7681 aactggcttt ctaattggag taaaacacaa tctaaggttg gatattgggt ttttaataag
     7741 gtaaaatcca ccaatattct ttttaacatt gcattttcgg tatatgattt acattcaacg
     7801 cccaaaacta attcattatc aatgtaaact ggaacatcta tattcagttc ataataataa
     7861 ttacccaaat gatcttgaat atatgatttt atttcaggat tttttattct ttttacataa
     7921 tcagctttta ttggtatacg aacttttgtc ttatttaaag ataaatgtgt ttgatctcca
     7981 tttaagcgat cccatgctat ctgaatcata cctttagcaa tattttctac taaactaccc
     8041 tttcctgctc taattattcc accataagct ctatctttac tatttaatgc ttccttatca
     8101 atgcctttaa taatagcatc atagatatta attaggtctt caacagtcgg aattttcata
     8161 atttcccacc aaattaatta aacaacctta aaatgtaaca tataaaaagg taattctata
     8221 ttatacttca tatcatctaa taataatagt ttaataatta aaataaaaag gatgataacc
     8281 tttcttcagc tttagaacaa tattcactgg aaatatcaat ccccaaataa tgccttttat
     8341 tcaattttgc cactaaggtt gtagttccta ctccgttaaa cgggtctaac acaatatctt
     8401 cttgatagct aaaaagttta ataactttgg tggcaagttc ttcgggaaac atagcaggat
     8461 gattaaattc tttcattctt ctttcaggag cgatagacca ttttgcatag acccattttt
     8521 taaactcatc tccgctaatg tcaatctttt tatcgtctcc gattttttta agagtgcctt
     8581 tacaaaatat ttctataaat tcccaagtat atttcagata gggattactt gggcttttcc
     8641 atgatcccca agctgtatat ttacaattat aattattttt ctcccataat atttctccct
     8701 tccagattag ctttttttgc attaaatagt tacttattat gtgatgagta ggaatataat
     8761 ctgaaaataa gggttgaaca ttgataatga atcttccacc atacttaaga actcgtatgc
     8821 attcatcaaa aatagcaaag agggtttcaa agtatttttc ccaatgctga tcatcctcat
     8881 tttcttcata atccaaacca aaattataag ggggagaagt aaaaattaaa tcaatacaat
     8941 tgttgggtaa ttgagatagt attattttac tatcgccaca tacgatccga tccatatatt
     9001 tatcaggcaa taagttattt atctcagaaa agtcgtttcc ccgagtataa tagaagattt
     9061 ctcgtttttt ttccttttct tttctattaa cagtttttct ttcatctcca tatcctacat
     9121 aaagacgttt ttttcccttt ataccataac tcttgatgtt acttatcgca cttgatattt
     9181 tatttaaatc attgttatta ttattaagtg ataattcttc tatatctgat ttgaacattt
     9241 caaaatcaat attattaaga taaactaaaa cttggttatc attataatca aaatgaatat
     9301 tactttcacc atatttcttt tctaaggcat tcaagatgct catagtataa ttatttatag
     9361 ggtaatcaaa ttcaaccttg atttccttat aattaaccat tcttacctct actttctttt
     9421 ttttaatatt atcatttctc ttcagttttt tatcactata tatagacatt catataatct
     9481 attattgagt tgtcaagtaa aatattctaa tttcaggttg ccttagtcag gaataatttt
     9541 aatttgatat tagatgtaat acaaactaca aaattataca atttccaatt tgacaaacca
     9601 tcttaaaaaa tacgcttagg gattttatca cttttttatg atttttgaag gattaattta
     9661 agctccgaaa gatatgcatt tgttattcct actaaagtag ttccagctat tcaatccaat
     9721 attccaagag aaatgaaaaa gttaagaaat gattttggtg ttaaattaat agttaaaaac
     9781 gagtggtgtt cttttaattt agctaatagg aaagccccat aatcgctatc ctgaaacgat
     9841 attttgttta tgattactgt tcataagcaa taaggagaaa aaatatacat aaggtattcc
     9901 ttacttattt actataagat agacaatttc cttgactgaa tctgcagttg tgttaaaaga
     9961 tataaaaaag caaagaagga ttgctaatgt cggaaatact gtcaatagat ataaaacgcc
    10021 ttttttatgg tgctcaagtt ttactaaatg agatacaatt aaaagtggaa aagggagaac
    10081 gaattttaat taccggaatt accggaagcg gaaaaagttc tctgctttct actttgaacc
    10141 tgatgaacag aaattatgaa ggcgaaattc tgtttgaagg gaagaatatt gtggaatatc
    10201 agcccgatat attacgcagt aagatttgta tggtgttaca agaaccctat ttggaagatg
    10261 ccaatgtgca agaggtttta gaccttcccc ttcattacag ttctttaaag cacaggcgtt
    10321 tacccgatag gcaggaaaga attaaaaatt tattcactgc atttcaattg tcttatgaat
    10381 atctgcaaaa accgacttct cagctttcgg ttggtgaaaa acaacgcatt gccttaatca
    10441 gagttctgca attgcagccg gatattttat tacttgatga aattacttca gctctggatc
    10501 aaaagacctc agggataatt tccgaatgta tttttaacaa ttttgcggga acagtaatag
    10561 ccatttccca cgatccgctt tggcaaaacc tttggcaaag aaattgggtt ctggaatatg
    10621 gaacaatcat agacaggagg atataaaata atggatatca gtatttatag attgatactg
    10681 gcagttttac ttttaatttt tccgcttttg atttttagca atctgaaact gaaattgagc
    10741 ggtcaacttt ttaattcttt tgccagaatg attgtgcaac tggcaattat tggcttaatt
    10801 ctacagttta tattcaacag agaaaatccg tggcttgcct ttctttggat gcttattatg
    10861 ttagcaaatg ctgttttaac cttaaaagga cggctcaaat ttcaaaaaaa aattctttta
    10921 ccagttctca tattttcact tttaactact acgctgattg ttatgccttg gctgataatt
    10981 gttgttctgc gtccagaacc cttgtttgct ccaaggtttc taattcccat ctatggtatg
    11041 attttaggaa acagtatgaa taattgttca cttgccctgg aacgttttga aagtggtctt
    11101 agcgaaaact ggaaagcata ttatacccgc ctttctttgg gtgcaagcca atgggaagct
    11161 atccttcctg ctttcagaaa agcaatgcag gcagctctaa tgccggaatt gcttacaatt
    11221 gcctctatgg gacttgtaac tttgccagga atgatgaccg gtcaaatttt aggtggtgct
    11281 tcccctctgg tagctattaa ataccaaatg atgattatga ttggtatctt cagtggagtt
    11341 acgattaccg attataccgc tataaacata tacctgagaa aaaggtttga taaattctat
    11401 ctgcccaaac cataaaaaat gaagataatt taggtttaaa agaaaaaatg aaacttgaaa
    11461 aatgaaattt ccaatttttt aagaatggta atctctatta aacggacaaa tagcaagtaa
    11521 tatatcaaag aatacatatt tggaaagtta taattactat tattacggaa atatgcttaa
    11581 acagaaataa acatcacaat tttatagaca aaattattac atataattat tgaagaacac
    11641 aagctcacaa attacttgac aagaaatttg attatttttg atagataaaa attctttcag
    11701 ataaaaaatt gagaggagaa aataacgatg gattatgatg tgtattgtga tgaaagttat
    11761 cctgatttat tcagttctaa gaaaccaaaa gcaaaatatc ttgtaatagg tagtttgtgg
    11821 ctatgcaaac aaaacagaaa tgtttttaaa aaggaaatac attctctccg cgataaacat
    11881 aaaatgggta gaggtgaatt taaatggaaa aaagtatcca aatcacgact tgctttttat
    11941 gaagaactgt tagattggtt tattgcaaaa ggtgatgaac ttagctttag atgcattata
    12001 gtagataatg ctaaaattaa tctggtagaa tttcataaag atgatcaaga attagggttt
    12061 tataaattct attatcaaat gttacatcac tggttagagg ataataattc ctacaatata
    12121 ttttgtgaca ataaaacaat aagtacgaaa agtagattaa taactctgcg cgaatgttta
    12181 agaaatgcta ataaatcatc ttgggttact gatattcaag cagtaacttc aaaggaatca
    12241 gttcttttgc aattgagcga tgtattagtc ggtttagctt cctccaagtt caataataac
    12301 cttaagcaag ggtccctaaa atccgaatta ataagctatt ttgaacaaac aatgggaaga
    12361 gaaattatgc caactagtaa agatgagaaa aaattcaatg tgtttaaaat taagttacgg
    12421 gacggtaggt aaatgaacta ccctccattt cttaatctta atagcattga tgaatataga
    12481 gcacattttg aaaaaactta ttgtagatgt ccacttcaaa catttgataa gtatatagta
    12541 agatttaaaa aaagcgattt tgagcatgcg ttttatgaaa gttccagaag aaatggaata
    12601 aaagataact tttccaaaaa tagagcattg agaatggact ggataaagta tgccttaatg
    12661 gattcaacaa gtatattaaa agtaggatgg gataaaaata aaaaaaatat ataaataaaa
    12721 ggagagtagc aatagttcaa aagaattatg ttataataat attagtaacg aatccatcaa
    12781 agaaaatagc ccgttttata acagcatttg tagctaaccg gtataccatc aacaaaattc
    12841 ttcaaagtcc tgaatggaaa taaaaaaccg tcgatttgac caggcgggtt cgacggcaac
    12901 ctttgttggt tcaattaccc caggtaatga acactgataa tataatcaga atgcatagtt
    12961 atgtcaagta aaatatttga tttttttctc ttctctttgc gtaactgctt atataacttt
    13021 ggtttcaagc atataaattt tttaaaaatt gggagtgttc attttattgg gtctgttttc
    13081 taataaaata atcggaaaac catag
//
LOCUS       DAOPFL010000043        12977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5654_length_12977_cov_108.017722, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000043 DAOPFL010000000
VERSION     DAOPFL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5654_length_12977_cov_108.017722"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..87
                     /locus_tag="PLG69_04435"
     tRNA            10..87
                     /locus_tag="PLG69_04435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Pro,seq:ggg)
     gene            1126..1713
                     /gene="infC"
                     /locus_tag="PLG69_04440"
     CDS             1126..1713
                     /gene="infC"
                     /locus_tag="PLG69_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPI42791.1"
                     /translation="MPKERINNQITAEKVRLIGPDGKQIGVISLKEALRKAENMDLDL
                     VEISPNAVPPVCRILDFSKYYFEKEKKAKEARKRQHEVEVKEIKFGPNTEEHDYNFKK
                     NNAIKFLKQHNKVKFTVRFRGRQMAHKELGYKVLDKLKEDLAHLVDIDGEPVADRNMI
                     SMVVSPKKDIDRILASETKKEETPIPAVEKSEPNT"
     gene            1806..2000
                     /gene="rpmI"
                     /locus_tag="PLG69_04445"
     CDS             1806..2000
                     /gene="rpmI"
                     /locus_tag="PLG69_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPI42792.1"
                     /translation="MPKLKTNRSAAKRFKITGTGKIVRHHAKSAHIKTKKSPKLKRYL
                     RSSEIVKKPDEKRIYRMLGL"
     gene            2016..2363
                     /gene="rplT"
                     /locus_tag="PLG69_04450"
     CDS             2016..2363
                     /gene="rplT"
                     /locus_tag="PLG69_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPI42793.1"
                     /translation="MPRTTNNVSAHRRRKKYMLQARGYYGSRSKTYRAARQTVERAMA
                     FSFAHRKQKKRQFRSLWITRINAACRMNDINYSQFINGLHKANIQINRKTLAHLAWHD
                     SPAFAKLVEIAKG"
     gene            2606..3640
                     /gene="pheS"
                     /locus_tag="PLG69_04455"
     CDS             2606..3640
                     /gene="pheS"
                     /locus_tag="PLG69_04455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPI42794.1"
                     /translation="MEEELRKLVEYAEKDIFACSSPNDLLNVKAKYLGKKSLLSSLYA
                     QMRNIPVEERPAFGNLINEAKKRIEDLLEQQNKVIKENAWKKNEAKVGTDLTMPGIFS
                     SRGGLHPLTIIRREIDEVFLGLGFEIAEGPDIDDDFHNFDALNTPLDHPSRNLADTFY
                     LEGGGLLRTHTSTVQVRVMEKYPPPIKIISPGRCYRNDKPDPSHSPVFHQVEALVVDK
                     GISLADMKDMLSQFASIMFGKDVSSRIRPHFFPFTEPSAEMDISCVVCGGTGCRVCKY
                     SGWLEMGGAGMVDPNVFDILGIDSEFYTGYAFGLGIERIAMLKYNIPDMRILYENDLR
                     MLRQFKGEFR"
     gene            3637..6192
                     /gene="pheT"
                     /locus_tag="PLG69_04460"
     CDS             3637..6192
                     /gene="pheT"
                     /locus_tag="PLG69_04460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPI42795.1"
                     /translation="MKIALSWLAKYIEIKENTKELEEILTFSGIEVEAVENIPALPET
                     VFSAKIISAEPVPETDHLKRCLVDIGNFPYSEKTEDGYLQVICGAPNCRTGMMAIIAL
                     PGSVLPEMTIAKAQIKGIDSYGMLCSEKELGISDNHSGIIELSTETPIGITANELYEL
                     PDTIFELEITPNRPDLLGYIGIARDLSAKLNRSLKMPEIKIPEAKCKAETMPLELVNK
                     VPELCPRYIARLFNNVELKESPLWLKSALIKSGLRPINNLVDITNYVMLEYGHPLHCF
                     DYDKLAALPEKPGIPAIVIRRAYQNEPIITLDGKSYLLEGQEMVIADGIKPSAIAGVM
                     GGNISAISETTKRIVLESAAFNPSSIRRTSYKHKISTDSSYRFERHLSEYSAEDASIR
                     ATQLICELANAELCEVVYDSYPIPVQPLIIGIRPSRYTQVIGYELEEEKIKDYLTRLG
                     LKFIQYGNWKPGKINSLAELYCHHTEEMKQGKTEFTELPNCEHTHYYEIPPYRVDLER
                     EIDIIEEIARLDGYDKIPVKTLPQRIMDRHSYRIKKNAVDYLISRGFYETLNYSFSEP
                     DLLYKLGYEDGDSELEMITLKNPQSSNQSGMRTSLIPQLLQNLAYNLNRGEKNIKLFE
                     LGKTYHRQEALSYEPYFLAGIVTGLNKEEHWQDKASPITLFWVKGIVEELLSLWYLEN
                     WSVQETKVPFLSRTESASYYLENKELAYYGKLNSVVAEKFDIDTIELKQDIWIVQFAM
                     ENIINATRNLKTHFQEIPRFPFVTRDISFLIAEEIPYSEIIKTIAEIERNIISEVTAF
                     DEYRGKQIPEGKRSLTLRLKFRDKEKTLTDERVDYLIDLIIKKLRETWQIKMR"
     gene            6196..6519
                     /locus_tag="PLG69_04465"
     CDS             6196..6519
                     /locus_tag="PLG69_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42796.1"
                     /translation="MESNVINLQDKIQKLIDQYTLATKKVEEVEEKNRQLAEENSQLI
                     AQIQTSGKATGEQEKKYKTLQEEYNTLENRYKELQKILAGLESVAEGAIKKIDGLFAS
                     SEKET"
     gene            6522..6785
                     /locus_tag="PLG69_04470"
     CDS             6522..6785
                     /locus_tag="PLG69_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPI42797.1"
                     /translation="MQSVEVEIFGRRFRLRSDDPERTKQIANEINDQIDALSKHYDNL
                     DFTKLLLLICLQQQDEVLTLDAKNRALSSELERLNQMLSKIKI"
     gene            7017..8585
                     /gene="rny"
                     /locus_tag="PLG69_04475"
     CDS             7017..8585
                     /gene="rny"
                     /locus_tag="PLG69_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPI42798.1"
                     /translation="MTINPIIALIIGVAGGFAIALIIYFIKRNTAFKLISQSTKIAEE
                     LEKNAKIEAENAKKAALLEAREEWIKQKRLLDEEIKERQKELRLEEKKYNERLSSLDK
                     RLDSLDKKENALAEQERKLKSKEEELLNKKNEFDAIIAEQKNKLAEIAGLSREEAIAR
                     LREELISQARQVAANDARLTIEQIKLDANRKAAEILSTAIQRMAVDYVSETTVSVVSL
                     PSDEMKGRIIGREGRNIRTFEKVSGVDLIIDDTPEAVVLSCFDPVRREVARLALEKLI
                     ADGRIHPGRIEEVIAKTAKEMEENLVKIGEKAVLETNIHNISPNLIKVLGRLHYRTSY
                     GQNVLEHSIESAWICGILAAELGLDQELARRAGLLHDIGKAIDHEFDGSHAIIGANLA
                     RKNGEGKIIVNAIEAHHEEVEAISVYAALTQASDAISGARPGARREMLETYMQRLSKL
                     EEIANSIDGVSKSYAIQAGRELRIIVEPSLIDENQTALLATEIAASIEKQVQYPGQIK
                     VTVIRETRQIAMAK"
     gene            8585..9394
                     /locus_tag="PLG69_04480"
     CDS             8585..9394
                     /locus_tag="PLG69_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPI42799.1"
                     /translation="MKVLFFGDVYGKAGRQTVQLTLPELKEEFQPDFILANCENLADG
                     KGVTEKTLNQLFNAGVDAVTGGNHLWDRTESLEYIRGQNRIIKPLNYPEDAPGNPYII
                     LAKENLRLGVLCLCGQLLMPPCNNPFTALSEFIKLHFTKDAEQVSLFPLPYPVILDFH
                     CEATSEKRAMGWFADGQLSAVLGTHTHIQTADEEILPLGTAYITDIGMTGSHNSVIGV
                     KKEIIIEKFRTSVPNRFESSDSGLQVNAVCLELDSETGKALNIMRIRRNIE"
     gene            9387..10310
                     /locus_tag="PLG69_04485"
     CDS             9387..10310
                     /locus_tag="PLG69_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPI42800.1"
                     /translation="MNKIMEKVLNGKPVAQLINRTCSSLIANYGLKPQMVLIQVGDDP
                     ASTYYVQSIINSGKKLGCFCQMKILPDNISEQELLAILNEANHNPDVNGIMLQKPLPK
                     HINESKVNSAINPEKDLDCLHPLNLGKIMMEEEGFLPCTPAAVLFCMLYYGIETQGKH
                     IVILGRSNVVGKPLANILLWKRNFANATVTVCHSKTNNLAEFTKEADILITAIGKAEF
                     VTPEMVKENAIILDVGINEIKLANGESNYVGDVNYKLCYDKALAITPVPGGIGRITTS
                     VLFLNLVDACLKSRNTNKSIDEYIHLIFDGK"
     gene            10342..12750
                     /locus_tag="PLG69_04490"
     CDS             10342..12750
                     /locus_tag="PLG69_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42801.1"
                     /translation="MMQLKSAKLLFIIVMIISSVLLISGCGKSGNRFANQPPTIQITS
                     FEGWDTTYVSAGYDTTEVYSFQQRIYWHATDPDGVITGYAFRILDENNNPVPTPGYEY
                     IDLTGELTPDNLLALGTGWVIHYMTGADQNIPLDDPQARRSIWTSQKYAVINFPSADS
                     LGNPIVKFSRFEVVAIDNRGAITPHPAWRNFRTQSDRPKCMISSTKGNPNGKDVGSGL
                     KLAFSMSDTDPFITAVPYKYEFKIMKINNQGEVISGTESDWFSTTGEDNRTNRINEYL
                     LTKYTNPPLSYDYDEVTLAFKNQRTKIISRVTDLAGVISIPDAASTMIFSVRPGFRPH
                     TFIYPTKTYALTNNHFEDYGDDTTEEIKVIPRVVTEGVTRYALPLFKDFEGKYTAVYS
                     TNIKLWIRWGWWGEYGETRGETVTYGDTIKIKTTPYNKKVDVVLDSQTGQNYFSEITA
                     FDLRLDGEPYNYPPYPFSKYGFTDQNGKKWLRLPVNSPLGQTVVLTAGQLPLPPATEP
                     GEHKFEVRVVDLQDEYDPTPVDFTFYLHRYIEPANRQGVLIIDDDPVSAQVNDALITQ
                     RYQAMLEGYSGNVNVITRTENNEDIRQRAIAFSDLQKYKLVIYHTDNYEKTGNLQLDF
                     DAYSLFLMRGGNLLISHTSLLGAQLTEIANGGLRKTFVTNLGFNKIPKVSYLNNSNSP
                     FFQKAVSNMTDYNDLNLHYDVTGSPAIHPLIDLRDGLGYLSYFENGNFSGDILYKFGC
                     KPTTYPTYPPTSEQFDKYNGKTVAFRRTTSSNGKVYVFGFPLSFMKVEDTRPMMNKII
                     SELM"
BASE COUNT         4260 a         2298 c         2672 g         3747 t
ORIGIN      
        1 taaccggttc gggatgtagc gcagtccggc tagcgtactt gaatggggtt caagtggtcg
       61 caggttcaaa tcctgtcatc ccgaccagtt tttttgtttc tcacagatta tacagataac
      121 agtttcacgc agatttcgca gatgacgcag atttgaatag gtaggggatg aacaggatta
      181 aaaggattta caggattttt tcttttattt accattaagt aatatcattt tttgaatgat
      241 aagccgtttt tcattactgc gaaagtagga atccacataa gctacaaaaa tatttatgca
      301 ttttcaatct gcacgatatg tgaaatcctg ctgattcagt catatgagtt ctattttttt
      361 agtcgtcacg agtcctcatc gttaagttaa tttgtgataa tgtgtaaaat tatataagca
      421 atgaggactc ctgacttaat tcattcttca ttttaacgag gggcttacgc caccttcaac
      481 atccttgtgt cgccctgacg ggcttttgga aaaggagaag ataaaaggaa aaaggaactc
      541 ggggcttaca ttttccaagg ggcttacgcc accatcgcta taattgtgtc gcccttcggg
      601 ctctaaagga attaacaaat ctaaaccttt taaaacctct aaactttcta aaccccctaa
      661 actttctcaa ccttctaaac tttattttcc gaggggctta cgctaccatc gctataattg
      721 tgtcgccctg acgggcttta aagtatctca attatctcaa ccatctcaac cttctaaacc
      781 ttatcaacct tctcaaccat tttcaattct tcattattct atttctcttt gtttttccat
      841 ctcgccattc tcttttcctc tttgtttcat acttcacaga aaaagtgatt gacaggactt
      901 ggcagggcaa aaaaaatgct cccgaaaatg aagctgcttg cttctacctt atggcttgat
      961 tattgtgctg ataaggttta aacccatata aaaaagaaat acaaaagtat ttactgtgaa
     1021 gcaggcggtt ttccgataaa aaagataacc gcttgttttg tttccggtaa gaaggaggtt
     1081 cttataatcc ggattaagaa aggaaaggca cgcacgcatg aagttgtgcc taaagaaaga
     1141 atcaataatc agataacggc agaaaaggtt cgtcttatag gtccagacgg aaaacagata
     1201 ggagttatat ctttgaaaga ggcattgcgt aaagcagaga atatggattt ggatttagtg
     1261 gaaatctctc ctaatgctgt tccgccagta tgtcggattc tggatttcag caagtattat
     1321 tttgagaagg aaaagaaggc aaaagaagcc cgaaaaagac agcatgaagt agaagtgaag
     1381 gaaatcaagt tcggtcccaa tacggaagaa cacgattata acttcaagaa aaacaatgct
     1441 atcaagttct tgaaacagca taataaggtt aaatttacag ttcgtttccg gggcagacaa
     1501 atggctcata aggaacttgg ctataaggtt ttggataaac tgaaagaaga ccttgctcac
     1561 ctggtagata ttgatggtga gccggttgct gacagaaata tgatttcaat ggttgtctca
     1621 cccaaaaagg atattgaccg tatcttagcc agtgaaacca aaaaggaaga aacacctatt
     1681 ccggctgtag aaaagtcgga accgaatact taaagacata ttgaaaatag aaacattccg
     1741 gaagttccct ttccgtaatg gaacctttaa taaagggaaa tgagtcacaa tataaggaga
     1801 taaaaatgcc taagctcaaa accaaccgtt cggcagctaa aagatttaaa attactggaa
     1861 cgggaaaaat tgtgcgtcac catgctaaaa gtgcgcacat taaaacaaaa aaatcgccta
     1921 aactgaaacg ctatctgcga agcagtgaaa tcgtcaaaaa gcccgacgaa aaaagaattt
     1981 acaggatgct gggtttataa ttaagggagg atgaaatgcc aagaacaact aataatgtat
     2041 ctgctcaccg cagaagaaaa aaatatatgc tacaagctcg tggctattat ggaagtagga
     2101 gcaaaacata tcgtgctgcc agacaaacag ttgaacgcgc tatggctttt tcttttgcac
     2161 atcgtaaaca gaaaaaacgc caatttcgga gtttgtggat aaccagaatt aatgctgcct
     2221 gcaggatgaa tgatatcaac tacagccaat ttattaacgg tctgcataag gcaaatattc
     2281 aaatcaaccg taaaacattg gcacatcttg cctggcatga ttctccggcg tttgctaaac
     2341 tggtagaaat tgctaaaggt taaaaaaagg taatagcacg tagattacgc aggtaaaaag
     2401 caatgggatt tcaagaatga aatagattta agggagaaaa aggtgtttaa ctatattact
     2461 atatatgcgt taagaaaaat tccgaacatt tttgggaatg ataaaaataa catccttttc
     2521 catccttata atctcataaa tcccttacct aataattaaa tctgcataat cagcgaaatc
     2581 tgcgtgcaga ttaaatgagg gaaaggtgga agaggaatta cgaaaactcg ttgaatatgc
     2641 agagaaagat atttttgctt gctccagccc caatgaccta ttgaatgtaa aagccaaata
     2701 tttgggcaaa aaaagtttgt tgagctctct ttatgctcaa atgagaaata taccggttga
     2761 agaacgtcct gctttcggca atttgataaa tgaagccaaa aaacgcatag aagacctttt
     2821 agaacaacag aataaagtca tcaaagaaaa tgcctggaaa aaaaacgaag ccaaagtagg
     2881 caccgatctt acaatgcctg ggattttcag ttcccggggt ggcttgcatc ctttaacaat
     2941 tatccgtcgt gaaattgatg aggtcttttt gggtttaggt tttgaaattg ctgagggacc
     3001 tgatattgat gatgattttc ataattttga tgctttaaac actcctttag atcatccttc
     3061 gcgtaatctt gccgatactt tttatctgga aggaggagga cttttaagaa ctcatacttc
     3121 tactgttcaa gtaagggtga tggaaaaata cccacctcca ataaagataa tttcccctgg
     3181 tcgttgttat agaaatgata agcccgatcc ttctcattcg cctgtctttc atcaggttga
     3241 ggcattagtt gtagataaag gtatttctct ggcagatatg aaagatatgc tgtctcaatt
     3301 tgcttcaatt atgtttggca aagatgtttc cagcagaatc agaccgcatt ttttcccctt
     3361 tacggaaccc agtgcggaaa tggatatttc ctgtgttgtt tgtggtggaa caggttgcag
     3421 ggtttgcaaa tattccggat ggttggaaat gggtggagcg ggaatggttg atcccaatgt
     3481 ttttgatata ttggggattg attccgaatt ttataccggt tatgcttttg gcttgggaat
     3541 tgaaaggata gcgatgttga aatacaatat tccggatatg cgtattttat atgaaaacga
     3601 tttaagaatg ttaaggcagt tcaaaggaga atttagatga agatagcttt gagctggctg
     3661 gcaaaatata ttgaaattaa agaaaatacg aaggaactgg aagaaatatt aactttttcg
     3721 ggtatagaag tagaagcagt ggaaaatatt cctgctttac cggaaaccgt tttcagcgct
     3781 aaaatcattt ctgcggaacc ggttccagaa acagaccatt tgaaacgctg tttggtagat
     3841 attggtaact ttccttactc cgaaaaaacg gaagatggct atctacaagt gatttgtgga
     3901 gctcccaatt gccgaacggg tatgatggct ataattgcgc ttccgggttc ggttttgcct
     3961 gaaatgacta ttgccaaagc ccaaattaaa ggcattgatt cctatggaat gctctgttcc
     4021 gaaaaagaat tgggaatttc agataatcat agcggtataa ttgaacttag tacagaaact
     4081 cccataggaa taactgctaa tgaactttat gaacttcccg atacaatttt tgaactggaa
     4141 attacaccca accgtcctga tcttcttggt tatatcggta ttgcgcgtga tctttccgct
     4201 aaattgaatc gttctttaaa aatgccggaa attaaaattc ccgaggcaaa atgcaaagct
     4261 gaaacaatgc ctttggaact ggttaataaa gttcccgaac tttgtccacg ctatattgcc
     4321 cgcttattta acaatgtgga attgaaagaa tcacctcttt ggctgaagag cgcattgata
     4381 aaatccggac ttcgtcctat aaacaacctt gtggatatta ccaattatgt aatgctggaa
     4441 tatggacatc ctttgcattg ttttgattat gataagcttg ctgcgcttcc tgaaaaacca
     4501 ggaataccgg caattgtaat tcgtagagca taccaaaatg aacccataat tactttagac
     4561 ggcaaaagct atcttttgga aggacaggaa atggttattg ctgatggcat taagccctct
     4621 gccatagcag gagttatggg tggaaatatt tctgccatca gtgaaactac aaaacgcata
     4681 gttttggaaa gtgccgcttt caatccgagt tccattcgga gaacttctta taagcataaa
     4741 atcagtaccg attcctccta tcgttttgaa aggcatttaa gtgaatattc tgctgaagat
     4801 gcctcaataa gggctactca gctgatttgt gaactggcaa atgcagaact ttgcgaagtt
     4861 gtttatgaca gttatcccat accagtgcaa cctttaatta taggcattcg tcccagtcgt
     4921 tatactcaag tgattggtta tgaactggag gaggaaaaga ttaaagatta tctcactcgt
     4981 ctgggactga aatttatcca atacgggaat tggaaaccgg gaaaaattaa ctctcttgcg
     5041 gaactatact gtcatcacac tgaagagatg aaacagggga aaaccgaatt tacggaactt
     5101 ccgaattgcg agcatactca ctactatgaa atacccccct atcgggttga cttggaacgc
     5161 gaaatagaca taattgagga aatagctcgt ttagacggtt atgacaaaat acctgtaaaa
     5221 acccttccac aaaggattat ggaccgtcat tcctatcgga taaagaaaaa tgctgtggat
     5281 tatttaatca gtcgcggctt ttacgagaca cttaattata gtttttccga accggatttg
     5341 ctctataaat tgggctatga agatggcgat agcgaactgg aaatgataac tttgaaaaat
     5401 ccgcaaagta gtaatcaaag cggtatgcgt acttctttga taccgcagtt attacaaaat
     5461 ttggcttata atctgaaccg tggcgaaaaa aatatcaaac tttttgagct gggaaagaca
     5521 tatcacaggc aggaagccct ctcttacgaa ccctactttt tagccggaat tgtaaccgga
     5581 ctgaataaag aagaacattg gcaggataaa gcaagcccga ttactttgtt ttgggtgaaa
     5641 ggaatcgtag aagaactgct ctccctttgg tatctggaaa attggtctgt tcaggaaact
     5701 aaagtgcctt ttctttcccg cactgaaagc gcctcttatt atctggaaaa taaggaactg
     5761 gcttattacg ggaaattaaa ttctgtagtg gcagaaaaat ttgatattga taccattgaa
     5821 ttaaaacagg atatttggat agttcaattc gctatggaaa acattatcaa tgctactcgc
     5881 aatcttaaaa cacactttca agaaattccc cgttttccct tcgtaacaag agatatttct
     5941 tttcttattg cggaagaaat tccctacagc gaaataataa aaacaattgc ggaaattgaa
     6001 agaaatataa tcagcgaagt aactgccttt gatgaatacc gtgggaaaca aattcctgaa
     6061 ggtaagagga gcttgacttt acggctgaaa tttagagaca aagaaaaaac attgacagat
     6121 gaacgagtag attatcttat tgatttaata ataaaaaagc taagggagac ctggcagata
     6181 aaaatgaggt aacttatgga aagtaatgta attaacctac aggacaagat acaaaaatta
     6241 atagaccagt atactctggc aactaaaaaa gttgaggaag tggaagagaa aaatcggcaa
     6301 cttgcagaag aaaattctca actgattgcc caaattcaaa ccagcggaaa agcaactggt
     6361 gaacaggaga aaaaatataa gacactgcag gaagaatata ataccctgga aaacaggtat
     6421 aaagaactgc agaaaatcct tgccggttta gaaagtgttg ctgaaggtgc tattaaaaag
     6481 attgacggat tattcgcctc cagcgaaaaa gagacctgat aatgcagtcg gtggaagtgg
     6541 aaatatttgg acgcagattt cgtttgcgta gtgatgatcc tgagcggaca aaacaaattg
     6601 caaacgaaat taacgaccaa atagatgcgc tttccaaaca ctacgataat ttggatttta
     6661 ctaaactgtt gcttttgatt tgtcttcagc aacaggatga agttttaact ttggacgcca
     6721 agaatcgtgc tttaagctcc gaacttgagc gtttgaatca gatgctttca aaaataaaga
     6781 tataaaaagt gcctgcagaa ttcgtgatat gggttgcata aaagctatta tagtttcggg
     6841 tgtcgagcca tgagtggcat gcgcgccagc tggtcctgga tacataaagc tcacaaggta
     6901 gatacccact tcaagcttgg ctttctaatg acaagcgtac acggttcatg cgggcacttg
     6961 aataattcgc tttggttata aatcaaagtt aaatagattg aaataaggaa ataacaatga
     7021 ctataaatcc tattatagca ctcatcatag gagtggcagg cggttttgcc attgctctta
     7081 ttatatattt cataaagcgt aatactgctt tcaaactgat ttcgcaatca actaaaattg
     7141 ccgaagagtt agaaaagaac gctaaaattg aagccgaaaa tgctaaaaaa gcagctctac
     7201 tggaagcacg cgaagaatgg attaaacaaa aacggcttct ggatgaggaa attaaagaac
     7261 gccaaaaaga actgcgcctg gaagaaaaaa aatacaatga acgtttaagc agtttggata
     7321 aacgcctgga ctctctggat aaaaaagaaa atgcccttgc ggaacaggaa agaaaattaa
     7381 aaagcaaaga agaagaactg ctcaataaga aaaatgaatt tgatgctatc attgctgaac
     7441 aaaaaaataa gctggctgaa attgcagggc tcagtcgtga agaagcaatt gcccgtttgc
     7501 gggaagaatt aatttcgcag gcaaggcagg ttgcggcaaa tgatgcccgg cttactattg
     7561 aacaaataaa actggatgcc aacagaaaag ctgcagaaat tctttccact gctattcaac
     7621 ggatggcagt agattatgta tccgaaacaa ccgtctctgt tgtatcgctt ccttccgatg
     7681 aaatgaaggg aagaataatt ggcagggaag gaagaaatat ccgcactttt gaaaaggtct
     7741 ccggagtgga cttaattatt gatgatactc ccgaggcagt tgtgctcagc tgttttgatc
     7801 ctgtccgtcg tgaagtagcc agattagctt tggaaaaact tattgccgat ggtagaattc
     7861 atcctggtcg cattgaagaa gtaatagcca aaacagctaa ggaaatggaa gagaatttgg
     7921 ttaagattgg agaaaaagca gttctggaaa ccaatataca caatatttca ccgaacctca
     7981 ttaaggtttt ggggcgtttg cattaccgaa ccagctacgg acaaaatgtt ttagagcact
     8041 caatagagtc cgcttggatt tgtggaattt tagctgctga gctgggtctg gatcaagaac
     8101 ttgctcgtag agcaggttta ttgcatgata taggaaaagc tatagaccac gaatttgacg
     8161 gttcccacgc tattattgga gccaatttgg cacgcaaaaa cggtgaggga aaaataattg
     8221 tgaatgctat tgaagctcac catgaagaag tggaggcaat ttctgtttat gccgctttaa
     8281 ctcaagcatc ggatgctatt tctggtgccc ggccaggtgc ccgaagagaa atgttggaaa
     8341 cctatatgca gagattatcc aaactggaag aaatagccaa tagtatagat ggagtaagta
     8401 aatcgtatgc cattcaggca ggcagggaat tacggataat tgtagagccc tctttgattg
     8461 atgaaaatca aactgcttta ttggcaaccg aaattgccgc ttctattgag aaacaggttc
     8521 agtatcccgg ccaaattaaa gttacggtta tcagagaaac acgccagata gcaatggcaa
     8581 agtaatgaaa gttctattct ttggtgatgt ttacggtaaa gcaggtaggc aaacagttca
     8641 gcttactctt ccggaattga aagaagaatt tcaaccggat tttattttag ctaattgtga
     8701 aaaccttgca gatgggaaag gagttaccga aaaaacactc aaccagcttt ttaatgcagg
     8761 agttgatgct gttacagggg gcaatcattt atgggataga acagaatctc tggaatatat
     8821 tcgcggtcaa aacagaataa ttaaaccgct taattatcct gaagatgctc cgggaaatcc
     8881 ttatattatt ttagctaagg aaaacctgcg tttgggagtt ctctgtcttt gtggtcaatt
     8941 gttgatgcca ccgtgtaata atccttttac agcattgtct gagtttataa agttacattt
     9001 tacaaaagac gctgaacagg tctccctttt tcctttgccc tatcctgtta ttttagattt
     9061 tcattgtgaa gcaaccagcg aaaaaagagc tatgggctgg tttgcggatg gacagcttag
     9121 tgctgtttta ggtactcata ctcatattca gaccgccgat gaagaaattt tgcctttggg
     9181 aacagcttat attacagata taggtatgac cggttcgcat aatagtgtaa tcggagtgaa
     9241 gaaagaaatt attattgaaa aattccgcac ttctgttcct aaccgttttg agtcctccga
     9301 ctcaggttta caggttaatg ctgtttgtct ggaattggat tccgaaacag ggaaagcatt
     9361 aaatattatg cgtataagaa gaaatattga ataaaattat ggagaaagtt ttaaacggaa
     9421 aacccgttgc ccaattgata aaccggactt gttccagttt aatagctaat tatggattga
     9481 aaccgcaaat ggttttaatc caggtagggg atgatcctgc ttccacttac tatgtccaaa
     9541 gcattatcaa tagcggaaag aaattgggtt gcttttgtca aatgaaaatc ctgccggata
     9601 acataagcga acaggaatta ttagcgatcc tgaacgaagc taatcacaat cccgatgtta
     9661 atggcataat gttacaaaaa ccgcttccta aacatataaa cgaaagcaaa gttaattcag
     9721 ctatcaatcc ggaaaaggat ttggactgtc tgcatcctct aaatttggga aaaataatga
     9781 tggaagaaga gggttttctt ccctgtactc ctgctgcagt tcttttttgt atgctatatt
     9841 atggaattga aacccaaggt aagcatatag taattttagg tcgcagtaat gtagttggca
     9901 agcccttagc taatattctg ctctggaaaa gaaattttgc gaatgctacg gttactgttt
     9961 gccatagcaa aacaaataac cttgctgagt ttaccaagga ggcagatatc ctcattacgg
    10021 caataggcaa agcggaattt gtaactcctg aaatggtaaa agaaaatgcc attattttgg
    10081 atgtgggaat aaatgaaatt aaattagcaa acggagaaag caattatgtg ggtgatgtta
    10141 attataaatt atgttacgat aaggcattgg ctataacacc tgttcccgga ggaataggca
    10201 gaattactac ctcagtgctc tttttgaatt tggttgatgc ctgtcttaaa tccagaaata
    10261 caaacaaaag tattgacgaa tatatccatc ttatttttga tggtaaataa tgaaatttac
    10321 taattccgat gtttgggagg aatgatgcag ttgaagagtg ctaaactctt attcatcata
    10381 gtgatgatta taagtagtgt attgctgatt tcgggctgcg gaaaatccgg aaaccgtttt
    10441 gccaatcagc caccaacaat tcaaataacc tcttttgagg gctgggatac aacttatgtt
    10501 agtgccggtt acgatacaac tgaggtttat agttttcagc agcgtattta ttggcatgct
    10561 actgatcctg atggagtaat taccggctat gcattcagaa ttttggatga aaataataat
    10621 cctgttccta ctcccggtta tgaatatata gatttaaccg gagagcttac ccctgataat
    10681 cttttagctt tgggaacggg atgggtaatt cactatatga ctggagcgga tcaaaatata
    10741 ccgcttgatg atccacaagc acgtcgttct atttggactt cgcaaaaata tgccgtaatc
    10801 aattttccct cagctgattc tctcgggaat ccgattgtta aatttagcag atttgaagtg
    10861 gtagctattg ataacagagg tgctattact cctcatcctg cctggaggaa tttccgtact
    10921 caatcggatc gtccaaaatg tatgatttct tccactaaag gtaacccgaa tggcaaagat
    10981 gtaggttccg gattaaaact tgccttctca atgagcgata ccgatccttt tattactgcg
    11041 gttccctata aatatgaatt taaaataatg aaaataaata accagggaga agtgatatca
    11101 ggaactgaat cggattggtt ttccactact ggtgaagata atagaactaa tagaattaat
    11161 gagtatctgc ttacaaaata tacaaacccg cctttatctt atgactatga tgaagtcaca
    11221 ttggcattca aaaaccaaag aaccaaaatt atatctcggg ttacagattt ggcaggagtt
    11281 atatctattc cggatgctgc atctacgatg attttctctg tccgtcctgg atttagacct
    11341 catactttta tctatcctac aaaaacctat gctctaacca acaatcattt tgaagattat
    11401 ggtgatgata ccaccgaaga aataaaagtt atacccagag tagttaccga gggagtaacg
    11461 cgttatgctc ttcctctctt taaggatttt gagggtaaat atactgctgt ctattctaca
    11521 aatattaaac tttggatccg ttggggctgg tggggcgaat atggagaaac aagaggggaa
    11581 actgttactt atggtgatac aattaaaatc aaaacaactc cttacaataa aaaagtggat
    11641 gtagttttgg atagccaaac agggcagaat tactttagcg aaataactgc ctttgatctt
    11701 cgtttagatg gtgaacccta taactatcct ccctatcctt ttagtaaata tggctttacc
    11761 gatcaaaacg ggaaaaaatg gttgcgcctt cctgtaaatt ctcctttggg acaaacagtc
    11821 gttcttacag ctggtcagtt acctcttcct ccagcaacag aaccgggaga acacaaattt
    11881 gaagttagag tagtagatct acaagatgaa tatgatccta ccccagtgga ttttactttc
    11941 tatttacatc gttacataga acctgctaac cggcagggag ttctgataat tgatgatgat
    12001 ccggttagtg ctcaagtaaa cgatgcacta attactcaaa ggtatcaagc tatgcttgaa
    12061 ggatatagcg gaaatgtaaa tgtaataacc agaacagaaa ataatgaaga tattcgtcaa
    12121 agagccattg cttttagcga cctccaaaaa tataaattag ttatctatca tactgacaat
    12181 tatgagaaga cgggtaattt gcaacttgac tttgatgcct atagtttatt cttaatgaga
    12241 ggcgggaatc tgttaatttc acacactagt cttttaggag cacaattaac ggaaattgct
    12301 aatggaggtt tacgcaaaac ctttgttacc aatctgggat ttaataaaat tcccaaagta
    12361 tcttacctaa ataattcaaa tagtcctttc tttcaaaaag ctgtttctaa tatgacagat
    12421 tataacgatt taaatttgca ttatgatgtt acgggttcac ctgccattca tccgcttatt
    12481 gatttacgtg atggtttagg ttatttatcc tattttgaga atggtaattt ttcgggtgat
    12541 attctttata aattcggttg taagcctaca acctatccta cttatccacc caccagcgag
    12601 caatttgata aatataatgg taaaacagtt gctttccgca gaacaacttc ttccaacggg
    12661 aaggtttatg tctttggctt tcctttatcc tttatgaaag ttgaagatac gcgtccgatg
    12721 atgaataaaa ttatttccga actgatgtaa ctttcattct tggaggaaaa agaactttgt
    12781 ctttttgaga gttcaaggtt caaagttgta acattctctc ttgaagggaa aaagaacttt
    12841 gtcattcctg ataaagcagg aatacacata aggttagcaa ggtatttatg tcttttcaac
    12901 ataaatacag taacatatcc tgctgatcca gtcatctgag ttctgttttt gggatcgtca
    12961 caagtcctca tcgctaa
//
LOCUS       DAOPFL010000044        12976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5656_length_12976_cov_110.523412, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000044 DAOPFL010000000
VERSION     DAOPFL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12976
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5656_length_12976_cov_110.523412"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..940
                     /locus_tag="PLG69_04495"
     CDS             152..940
                     /locus_tag="PLG69_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPI42802.1"
                     /translation="MSKFYLFCFAVVLVLMAVPLFSAEEDNFLQDYLLSYPETIYAGA
                     TAPFSWQKDNWLQASAIVFTGGCLYFLDDDLRDCFQRNRNEVTDDIFTVAKQFGEVKI
                     MFPVMAVTALGGYLTKDEKTIDTSLLCLKSSLLSLAVTQTLKLASQRQKPCKEEGKEF
                     WNGNGISFSRDSFPSGHTTLVWSLAPVLAEQYKNSRWVPPTVYTIATLTSLSRLNDDQ
                     HWSSDVFAGAVIGYVTARLVLKNTPRLMLNAQPEINGLSFKYKF"
     gene            complement(1106..2113)
                     /locus_tag="PLG69_04500"
     CDS             complement(1106..2113)
                     /locus_tag="PLG69_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPI42803.1"
                     /translation="MPEKRPQTSKLLYLPWIIKHLSYYLKLNTHILKKGVPLKEILIL
                     KYPFWFKDAKNPPLLSVDITDACNLRCIYCNNPFFPYPRTMMNQDVFKALIERLKRHP
                     VSRIRISGGEPTLHPQFDTMLKEMSYYCKYLSIITNGQWQNPEVNEQLLNCGLSFIEL
                     SMDAGGKEIYEKSRPGADYDLFFQNLKQLYKLRNQNKVDLIIRIRLMVRPSTMDKEKE
                     DSRFLRQFCDCVLPQHILQHPETPSQEDVFIQNSILQNSLPVCTVPYRDLQIRPDGLV
                     PLCPAKGCAINPEDRIFIGDIQKDEILDLWNAPLLKQMRDAHRNRKGEILKLCRNCHY
                     S"
     gene            complement(2120..4843)
                     /locus_tag="PLG69_04505"
     CDS             complement(2120..4843)
                     /locus_tag="PLG69_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPI42804.1"
                     /translation="MNRKYTLFLLILLSCGLLFAQTALSCYDIQYNPEGGGDSPYLDQ
                     IVTVQGIVSGVTYYSGSGYNNYGFFISDQTGGPWSGLFIYNQTYHPALGDFVRVTGTV
                     KEYYGLTEISQVSSLQVLSQGNPLPAPTEINTGSLDNFSSGEQWESVLVKVVNVTVTI
                     EPNSYQEFYVDDGSGQCQIDNQFFSSNHSWNNITLGQVYSEIIGIVDYSYSYYAINPR
                     SETDMISGANTVTLAIPHQSVGLHSSVSVPINAYRIETSQNYQSYSFHISFNPHILNY
                     ESINTAGTLSQAGNVIASAESGVLSVSYQSNSILNGQGILLKLNFYAANTGTTALTFS
                     DVIFGADVIQSCINGSVTVNSSYNSPGDTLTVIQRPLLNIPEIVVPGETLKITCLAPQ
                     NTSNWNAWLRHNNKRLNLPVTNSQWLTLPNRWELTTTVPSVPVYELYDLEVSASGGIS
                     DITANAVQVIPSRKNNYYFVHITDSHMPNRLYYPNAGFDADSTAVEDFRAVMDDINII
                     HPEFVLFTGDLINEGELEGFANQYWFGWTQKLLTEFEVPVYVTAGNHDIGGWNQTPPP
                     AGSARKNWWKYFGWSWLNNEDLSFPYHTQDYFFTYNNTVFIGLESYDNYDYWRPTIYG
                     ETSYTDQQIAWLNNTLSLFPNYKKVLFHHYDFKEQLVLSSLGIDLSLWGHIHSNNGSI
                     YTYPYNLATRSVCDGNRAYRVIRVNNEQLTPYATIYAGATGNNLSVQFYPSNYGVADS
                     VKAIIINNQPLAFENAQLKFIMPQRDTGYNVSGGTLTQVDKSGNYNICYVRVSLSANS
                     ISTVTVTENGVEISDPVQVPVVFTIKSVYPNPLVKQGNLQVISDKTFPELHLQLFNLR
                     GQLIYQECLKGISQGESHLSFTLPDSLASGIYFLRFREDKTQIHKILILQR"
     gene            complement(4840..5676)
                     /locus_tag="PLG69_04510"
     CDS             complement(4840..5676)
                     /locus_tag="PLG69_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPI42805.1"
                     /translation="MKRINLFTIILILLLNAFITMALINKYGNKTPETDTFVSQGTSQ
                     PSKASSAKRINSITEAVKAVEPAVVSINVIKTEYVRAVSPFGFGFFDFFGSIPLLRQV
                     ESIGSGVIYDPKGYIVTNAHVVSGATQIKVILPDKREFSGIVAGIDEIHDVAKIKITG
                     NNLPVAEMGNSNDLIIGEWSIALGNPYGFLMNDSKPSVSVGVISAVNRNFAARQDRRE
                     YKSMIQTDAAINPGNSGGPLVNINGEVVGINTFIFSENGGNIGIGFAIPINSVKTILA
                     KI"
     gene            complement(5689..6441)
                     /locus_tag="PLG69_04515"
     CDS             complement(5689..6441)
                     /locus_tag="PLG69_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HPI42806.1"
                     /translation="MKKEYFSIIKFPVLISFSLLIITLIFFFIVLGSYHRLGLSVWGN
                     VLYTILFIVIVILIGTWILALISTHRKIKPLWLRHYLSWMLVNIYYPLARVFGRICFQ
                     KMDTIQESFLYFNNEIVVTNYQGMHSSNLLLLLPHCLQSSNCKIRINNSIVECQECGG
                     CDMAKMKALTKDYQIKTAVASGGSLARKLVNDTHPDIIIAVACNRDLTEGVRESWRYP
                     VYAVLNERPKGPCFETTVSPAVIDFAIHKFVQ"
     gene            complement(6438..7382)
                     /gene="fmt"
                     /locus_tag="PLG69_04520"
     CDS             complement(6438..7382)
                     /gene="fmt"
                     /locus_tag="PLG69_04520"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPI42807.1"
                     /translation="MRYLFIGSSDYGLPALNKLRQNGYEPSLIISQPAKPAGRNLHLT
                     PTPVSQYAIEQKLPLFTPVDINSAESIAKMAQQKADIIVTAAFGEFINKKIRNLCPFG
                     AVNLHPSLLPKYRGASPIQSALLNGETETGTTISLVSAKMDAGPILAQTKLSIAENET
                     YSELKERLAEQAGDLLLQFWKKLITEKSLCGTKQDESKATYCYKITNKTCQIDWNKRT
                     EEIHNQIRAFSLTPGAWTLFRNNKLKILCSEKTEEPANGEPGLICKIEKKRGFFVNCL
                     DFKLLITKVQPAGKKVMEAVAFINGARLREKEKLGGEK"
     gene            complement(7382..7942)
                     /gene="def"
                     /locus_tag="PLG69_04525"
     CDS             complement(7382..7942)
                     /gene="def"
                     /locus_tag="PLG69_04525"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPI42808.1"
                     /translation="MKYKPKILPVRLYGDDFLRKKLPEIDYNTPGLQEFIEDLIYTMY
                     ARDGIGIAANQVGSFYRMIVIDPEQDNKLNKKSPIVMINPVIENREGEVVYEEGCISL
                     PDVFADVSRSKKITYSYTDRMGNRITETAEEIKAVVIQHEFDHLEGILFIDHLGTLDR
                     LKIMHKLKKLQARAVNGQNILEGLSL"
     gene            complement(7939..8280)
                     /gene="yajC"
                     /locus_tag="PLG69_04530"
     CDS             complement(7939..8280)
                     /gene="yajC"
                     /locus_tag="PLG69_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPI42809.1"
                     /translation="MNYILLQAAQKGTTQQGGAGTTLIFMIIMFVILYLLLIRPQQKK
                     AKETQKMQDSLKVNDKVITSSGIYGRVVSIKPDKGIVVLEIDDTNKVRIDIQKNAIIG
                     VVTTSEPEPEK"
     gene            complement(8289..8786)
                     /locus_tag="PLG69_04535"
     CDS             complement(8289..8786)
                     /locus_tag="PLG69_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPI42810.1"
                     /translation="MTAEKPQKLNIYTFLATLCGIGFIPVMPGTFGSLLAYGIYMLFS
                     GSPFQGKALFYALPALIVLCLIAVFLSTKAEKTLGKDNGSIVIDEFCGYYVSVLFLPH
                     NWLIGLYAFALFRVFDIAKPFPIKKVQELPGGWGVVTDDIIAGIYTNILIQILTKIYP
                     KFFGL"
     gene            complement(8783..10039)
                     /locus_tag="PLG69_04540"
     CDS             complement(8783..10039)
                     /locus_tag="PLG69_04540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPI42811.1"
                     /translation="MRKSAIILILLVLTLSLSAELIDKIVAKVGTDIILLSDLQKEMV
                     QMQSAGLLEENTDPRDVLDEMINQKLIIQKAKDLNITVNNDEIKAMAENYLKKVKAQY
                     PSSSAFAADLKKSKLTESDLLQLYRDILTEQALSDQILKKEIINKVSVTEAEVINFYN
                     ATKDSLAVKPVSWDLGIIFREIKPSQESKEAKLAEIKEIQRRLKNGEDFATLASTESD
                     CPSKEVGGDLGFFKRGQMVKPFEDAAFALQLGEISDIVESEYGYHIIRLEEKRGNEIR
                     ARHILKALTPTAEDSLRERQLMEEIRNRYAKGESFASLAREYSMDQESREDGGSLGEF
                     TERDLPSLFATQILQTPVGEMTPVLENQGMLYIFCRLKEYPPRIYKYEEVKDQAREMV
                     LKRKQIDAFNAWIENLRREAYVQITL"
     gene            complement(10054..10776)
                     /locus_tag="PLG69_04545"
     CDS             complement(10054..10776)
                     /locus_tag="PLG69_04545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPI42812.1"
                     /translation="MAQVDKEILYLDDFKSTFSVEDWENLSPEIRKKYIEDWVNLTLL
                     AHYADKQGLNKDQAVKQKIYYASKKVKANALIAKRLSEIEVSEDQLFNYFRLHQAEFQ
                     KNAVEYNIRRIALMDKISAENVLQQLSQGMSFTEAVQRYSTEELKNRGGFMGMVSATG
                     PDSLFWLKARELKENEYGLVYKEPMWYVFQIVESKITPQEANFEDYRAEIKRKVLLEK
                     QDQVYQELIKEIKSQTSEIYYY"
     gene            complement(10856..12070)
                     /locus_tag="PLG69_04550"
     CDS             complement(10856..12070)
                     /locus_tag="PLG69_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42813.1"
                     /translation="MELLPKVLKLLLCIIILVIMLSGCTDKKNLTGDNFSDIRAITIE
                     DKPGIIMGFSFPAETKEEITGKEAKLLAGNYQNAVAICLLRFTELPSPITETDSCFLT
                     INISKRSALPRNPLILKLCKLNCVWLDSLNLNDISEDDLSFLAEYTVADTIGSSGKVI
                     TFNLSSSDVVNWSNPDSTGWNFVLKTENEGWVEITSLEGTNKPTLNFKYKTETGGEWI
                     TYNKSPAKDTYILEAPETNPSVLWKISNLNSSRLYIKYEPTYTLFKDNEGNTLTDIQL
                     KRLTVNKAEIVLHIKNNDYYNSSTSYSLYPFNVVKDSIDTPVTLVKSDYEKLAYIDYS
                     IGFIKGDSLTVDVTPIIQAYTSGEKIPKGIMIQSTQERRNFGTLEFWDCNESTPAEKK
                     PYIKITYTPPYL"
     gene            complement(12491..>12976)
                     /locus_tag="PLG69_04555"
     CDS             complement(12491..>12976)
                     /locus_tag="PLG69_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPI42814.1"
                     /translation="EPENVVLSDAIFNFIADNITSSVRALEGSLIRILAFSSYNKLNP
                     EDIDINLAAEILSDMISEKVHSITLDAITEKVCECYGITPAQLLEKTRKPQVAFPRQV
                     AMYLANYLIPQLSLKDIAEYYNRKDHTTVLHAKKMVENQFREKEDFRIQLEEIIKNLK
                     K"
BASE COUNT         3831 a         2471 c         2438 g         4236 t
ORIGIN      
        1 tatctgtcgt tcctctggaa ctttttttca agataaggga taataattca gatatgttct
       61 gttcggtgtt ttcggtggct gaaaacaatc aatctatgaa cattattgtg tttaatcttg
      121 acagttgtcc ccaattttag aagatagata aatgagtaaa ttttatcttt tctgttttgc
      181 tgtggtcttg gtgctaatgg cggttccgtt attttctgca gaggaagata actttttgca
      241 agattatttg ttatcctatc ctgaaacaat ttatgccgga gctacggcac cttttagttg
      301 gcaaaaggat aattggttgc aagcaagtgc aatagttttt accggaggtt gtttatattt
      361 tctggatgat gatttgcggg attgctttca acggaacaga aatgaagtta cagatgatat
      421 ttttacggtt gccaaacaat tcggagaagt aaaaattatg tttccggtaa tggcggtaac
      481 tgctttagga ggatatctaa ctaaagatga aaagacaata gatacctctc tgctatgcct
      541 gaaaagttca ctcctctctt tagcggttac ccaaactttg aagctggctt cccaaaggca
      601 aaaaccctgt aaagaagaag gaaaagaatt ttggaacggc aacggaattt ctttttcccg
      661 ggatagtttt ccttcgggac atacaacttt ggtttggagt ttggcacctg ttttggcaga
      721 acaatacaaa aattcccgct gggtgcctcc aactgtttat acaattgcca ctttaacttc
      781 cctttcgcgt ttgaatgatg atcagcattg gagcagcgat gtttttgccg gtgccgttat
      841 cggttatgta acagcaagat tagtcctgaa aaatacacct cgtctgatgc tgaatgcaca
      901 acccgaaata aacggtcttt cttttaagta taaattctga agcataattt ttgggagtgg
      961 tttacctaac cttctaaagc atttatttcg ctctttgctt ttcaaggaat caaccttttt
     1021 taagaaggat tgatgaagat gacaacctat ctgtatcctt aattttcaaa accctctaaa
     1081 ccttcaaacc ttctcaaccc tcttttcagc tataatggca attcctacaa agctttaata
     1141 tttctccctt tctgttgcga tgcgcatccc gcatttgttt tagcaaaggt gcattccaga
     1201 gatcaagaat ttcatctttt tgaatgtctc caataaaaat acggtcttca ggattgatag
     1261 cgcaaccttt tgctggacaa agaggaacca aaccatcagg tcttatttgg aggtctctat
     1321 agggaacagt gcaaacagga agtgaatttt gcaggataga attttggata aaaacatctt
     1381 cttgggaagg tgtttcggga tgctgtaaaa tatgttgcgg taaaacacaa tcgcagaatt
     1441 ggcgtaaaaa acggctatct tccttttctt tatccatagt tgaaggacgc accattaacc
     1501 gaatgcggat gatgaggtca actttatttt gatttctcag tttataaagc tgtttcaggt
     1561 tttggaagaa aaggtcgtaa tcggcaccag gacgactttt ttcgtagatt tcttttccgc
     1621 cggcatccat agaaagttca ataaagctaa gtccgcaatt aaggagttgt tcattcactt
     1681 caggattttg ccattgccca ttggtgatga tagataaata tttacagtaa taggacattt
     1741 ctttcagcat tgtatcaaat tgaggatgca gggttggctc acctccactg attctgattc
     1801 tgcttacagg atggcgtttc aggcgctcaa ttaaggcctt aaaaacatcc tgattcatca
     1861 ttgttctcgg ataagggaaa aagggattat tacaataaat gcagcgtaaa ttgcaggcat
     1921 cggtaatatc tacggaaagc agaggaggat ttttggcatc tttgaaccag aaaggatatt
     1981 tcagaatcag tatttccttt aagggaacac cttttttcaa gatgtgagta ttcagtttta
     2041 agtaatagga aaggtgcttg ataatccacg ggagatagag gagttttgag gtttggggtc
     2101 ttttctcagg cataagatat tacctttgca aaatgaggat tttgtgaatt tgggttttat
     2161 cttcccgaaa acgcagaaag taaattccgc tggctaagga atcgggtaag gtaaaagaaa
     2221 gatgactttc accttgagaa attcctttta ggcattcctg ataaatcagt tgccctctca
     2281 ggttaaaaag ctgcaggtga agctccggaa atgtcttatc gctaataact tgcagatttc
     2341 cctgctttac caaaggatta ggataaacgg attttattgt gaatacaaca ggaacttgaa
     2401 cagggtcgga tatttccact ccgttttctg ttacggtaac agtggaaatg ctattggcag
     2461 aaagggaaac acgcacataa cagatattat aattgccact cttatctacc tgggtaagag
     2521 ttcctcctga aacattataa cccgtatctc gttgaggcat aataaatttc agctgtgcat
     2581 tttcaaaagc aaggggctga ttattgataa taattgcctt cacactatcc gcaactccat
     2641 aattggaagg ataaaattga acagaaaggt tattgcctgt tgctccggca taaattgtag
     2701 cataaggagt caattgctca ttattaactc tgataacacg ataagcacgg ttgccatcac
     2761 aaacactacg cgtagctaaa ttatagggat aagtataaat tgaaccgtta ttggaatgaa
     2821 tatgacccca aagagacaga tctattccca atgaggaaag aacaagctgt tccttaaaat
     2881 cgtaatggtg gaaaagaacc tttttgtaat tcggaaaaag agaaagggta ttattcagcc
     2941 aggcaatttg ctgatcagta tagcttgttt cgccataaat agttggccgc cagtaatcat
     3001 aattgtcata gctctctaac cctataaaaa cagtattatt ataggtaaaa aagtaatcct
     3061 gtgtatgata gggaaagctc aggtcttcat tatttagcca gctccaacca aaatatttcc
     3121 accagttttt tcgggcactt cctgcaggtg gaggggtttg attccaaccg ccaatatcat
     3181 gatttccggc agtaacataa accggaactt caaattcggt gagcaatttt tgcgtccagc
     3241 caaaccaata ttgattggca aagccctcca gctctccttc gttaattaaa tcgccggtaa
     3301 aaagaacaaa ctcgggatga ataatattaa tgtcgtccat cacggctcta aaatcttcta
     3361 cggcagtaga atcagcatca aaacctgcat taggataata cagacgattg ggcatatgcg
     3421 aatctgttat atggacaaaa tagtaattgt tttttcggct gggaataacc tgaacagcat
     3481 tagctgtaat atcgcttata cctccactgg cagagacctc caaatcgtat aattcataaa
     3541 caggaacaga aggaaccgtt gttgtaagtt cccaacggtt tggtaaagtt aaccattggc
     3601 tgtttgtaac cggtaaattc aggcgtttat tgttatgtct cagccaagca ttccagttgc
     3661 ttgtattttg aggggctaaa caggttattt ttaatgtttc tccgggaaca actatttccg
     3721 gaatattgag aagaggacgc tggataacgg ttaaagtatc gccaggtgaa ttataactgc
     3781 tatttacagt tacacttcca ttgatacagc tttgaattac atcggctccg aatattacat
     3841 ccgaaaaagt tagcgcagtc gttcctgtat tggcagcgta aaaattcagt tttaaaagaa
     3901 ttccctgccc gtttaaaatt gaattgcttt gataagaaac agataacact ccgctttccg
     3961 cagaagcaat tacatttcct gcctgggata aagtgcctgc agtgttgatt gattcataat
     4021 tcagaatatg gggattgaaa gaaatgtgga aagaatagga ttgataattc tgtgatgtct
     4081 ctatacgata agcatttata ggaacggaaa cagaactgtg caaaccgact gattgatgag
     4141 gaatagccaa agttactgtg ttagctcctg aaatcatatc tgtttccgaa cgcggattga
     4201 tggcatagta gctgtaagaa taatccacaa tcccgattat ttcgctgtag acctgtccta
     4261 atgtaatatt attccagctg tgattagagc taaagaattg attatctatc tggcattgcc
     4321 cactgccgtc atctacataa aactcctgat aactattggg ttcaattgtt acagtaacat
     4381 ttacgacctt caccaaaacg ctttcccatt gttctccact gctgaaatta tccagagaac
     4441 cggtatttat ttcagtgggg gcaggaagag gattgccctg gcttaaaacc tgaagggaag
     4501 atacctgaga aatttctgtt aagccgtaat attcttttac cgttccggtt acgcgcacaa
     4561 aatctcctaa agcaggatga taggtttgat tatagataaa taatccgctc cagggacccc
     4621 ctgtttgatc actaatgaag aaaccgtaat tgttataacc gcttccggaa taataggtaa
     4681 cgccacttac aattccttga acggtaacaa tttgatccaa ataaggtgaa tcgcctcccc
     4741 cctctggatt atactgaata tcatagcaac tgagtgctgt ttgggcaaaa agcaaaccgc
     4801 aggaaagcaa tatcagcaga aataaggtat attttctgtt catatctttg ctaaaatggt
     4861 ctttacggaa tttataggaa tagcaaagcc gataccgata ttgcctccat tttcggaaaa
     4921 gataaaggta ttaataccaa ctacttctcc attgatatta acaaggggac caccactgtt
     4981 tccaggattg atagcagcgt ccgtttgaat catagattta tattcacgcc tgtcttgacg
     5041 ggcagcaaaa ttacgattca cagcagaaat cactcctacg gaaacactgg gcttggaatc
     5101 attcattaaa aaaccataag gattacctaa agcaatactc cattctccga taattaaatc
     5161 gttggaattg cccatttcgg caacaggaag attatttccg gtgattttta ttttggcaac
     5221 atcgtgaatt tcgtcaattc cagcaacgat tccggaaaat tcgcgtttat caggtaaaat
     5281 aactttaatt tgagtagctc cgctaactac atgggcattg gtaacgatgt aacctttagg
     5341 atcataaata acaccgctgc caatggactc tacctgacgc agtaaaggta tggaaccgaa
     5401 gaaatcaaaa aaaccgaaac caaaaggaga tacggctcgc acatattctg tttttataac
     5461 attgatgcta acaactgccg gctccactgc ttttacagct tcggtaatag aatttatcct
     5521 tttagcagaa gatgctttac tcggttgaga agttccctgc gaaacgaagg tatcggtttc
     5581 tggtgtttta tttccgtatt tattgattaa agccatagta ataaaggcat taagcaatag
     5641 aataaggatt attgtgaaga gattgatgcg tttcaagttt tctctccttt attgaacaaa
     5701 tttatggatg gcaaagtcaa ttactgcagg acttacagtg gtttcaaaac aaggaccttt
     5761 ggggcgttca ttcaaaactg cataaacagg gtaacgccag ctttcacgaa ctccttctgt
     5821 taagtcccga ttgcaggcaa cagcaattat aatatccgga tgagtatcat ttaccaattt
     5881 tcttgccaaa gaaccaccac tggcaactgc agttttaatt tgatagtcct tggttaatgc
     5941 cttcatttta gccatatcac agccaccgca ttcctgacat tctacaatgc tgttatttat
     6001 gcgaatcttg cagttggaac tttgcaggca atgaggcaaa agtaaaagca gattggaaga
     6061 atgcattccc tgatagttgg taactacaat ttcattatta aaatagagaa atgactcctg
     6121 tatggtgtcc attttttgaa aacaaatcct gccaaaaact ctcgctaaag gataatagat
     6181 attcaccagc atccaggaaa gataatgtct cagccaaagg ggcttgattt ttctgtgggt
     6241 gctaataaga gctaaaatcc aagtgccgat taaaattaca atcacaataa agagtattgt
     6301 atagagaaca tttccccaga cagaaagacc taaacggtga taactgccta acactataaa
     6361 gaagaagatg agcgttataa taagcaaaga aaagctgata agcacaggga atttgattat
     6421 agaaaagtat tcttttttca tttttcccct cctaattttt ctttttctct taggcgggct
     6481 ccatttataa aagcaactgc ttccattact ttctttcccg ccggctgaac tttagtaata
     6541 agcaatttaa agtccagaca gttaacaaaa aaaccgcgct ttttctctat cttgcaaata
     6601 agccccggtt caccatttgc cggttcttct gtcttttctg aacacaatat cttcaattta
     6661 ttatttctaa acagagtcca ggcaccggga gttaaagaaa aagcgcgaat ttggttatga
     6721 atttcctcag ttcttttatt ccagtcaatt tggcaggttt tattggttat tttatagcag
     6781 taagttgcct tgctttcatc ctgcttcgtt ccgcaaagtg atttttccgt tataagtttc
     6841 ttccaaaact ggagtagtaa atcacctgcc tgttccgcta ggcgttcttt cagttcgctg
     6901 taagtttcat tttcagctat ggaaagcttt gtctgagcca gaataggtcc tgcatccatt
     6961 tttgcggaaa caagggatat tgtagttccg gtttcagttt ctccatttaa aagggcactt
     7021 tgaataggac tggcacctct atatttgggc aataatgaag gatgcagatt tacagctcca
     7081 aaagggcaga gattacggat ttttttatta ataaattcgc caaaggcagc agtaacaatt
     7141 atatctgcct tttgttgtgc catttttgct atactttctg ctgaatttat gtctacggga
     7201 gtaaaaagcg gtaatttttg ttcaatggca tattgtgata caggagttgg agttaagtgt
     7261 aaattgcgtc ctgcaggttt agccggctgg ctgattatta aagagggttc ataaccattt
     7321 tgccgtaatt tattcagtgc cggtaaaccg taatctgaag aaccaataaa caggtaacgc
     7381 attataaaga aagtccttcc agtatattct gtccgttaac cgctcttgcc tgtagttttt
     7441 taagtttgtg cattattttc aagcggtcaa gagttcccaa atggtcaata aataaaatac
     7501 cttccagatg gtcaaattcg tgttgaataa caactgcctt tatttcttca gcggtttccg
     7561 ttatgcgatt tcccattcta tccgtgtagg aataggtaat ttttttactg cgtgacacat
     7621 ctgcaaagac atctggtaaa ctgatacagc cctcttcgta aacaacttcg ccttctctgt
     7681 tttcaattac agggttaatc atcactatcg gacttttttt attcaactta ttatcctgtt
     7741 ctgggtctat aacaatcatt cggtagaaag agccaacctg attagcagca atgcctattc
     7801 catctcttgc atacatagtg tagattaaat cttcaataaa ttcttgcaga cctggagtat
     7861 tataatcaat ttctggcagt ttctttctta aaaaatcatc cccatagaga cgcacaggta
     7921 agattttggg cttatatttc atttttccgg ttcgggttca gatgttgtaa caactcctat
     7981 gatggcgttt ttttgaatat ctatccgcac tttattggta tcatcaattt ccaaaaccac
     8041 aattccttta tcgggtttta tggaaacgac gcgaccatag attccggaac tggttatgac
     8101 cttatcgttt acttttagtg aatcctgcat cttttgggtt tccttggctt tcttttgttg
     8161 gggtctgata agcaagagat acagaataac gaacattatg atcataaaga tcaaagttgt
     8221 gcctgcacca ccttgttgag tagtgccttt ttgggctgcc tgtagtaata tgtagttcat
     8281 tttttctcct ataatccaaa gaattttgga tatattttag ttagtatttg aataagtata
     8341 ttggtataaa ttccggcaat aatatcatcc gtaacaactc cccagccacc gggaagttcc
     8401 tgaacttttt taataggaaa gggtttggct atatcaaaaa cccggaaaag ggcaaaagca
     8461 taaaggccta ttagccaatt gtgaggtaaa aacaaaacgc ttacataata accgcagaat
     8521 tcatctatta caatacttcc gttatctttt cccagggttt tttccgcttt agtgcttaaa
     8581 aaaacagcta ttaaacaaag cactattaag gcaggcaaag cataaaacag tgccttccct
     8641 tgaaaaggtg aaccggaaaa gagcatataa attccataag caagaagaga accaaaagtt
     8701 cccggcataa caggaataaa gccaattccg caaagtgttg ctaaaaaggt ataaatattc
     8761 agcttttggg gtttttctgc agtcataaag ttatttgtac atacgcctca cgccgtaaat
     8821 tttctatcca ggcattaaag gcatcaatct gttttctttt aagaaccatc tcccgagctt
     8881 ggtcttttac ttcttcatat ttataaatgc ggggagggta ttctttcaag cggcagaaaa
     8941 tgtaaagcat accctgattt tccagaacgg gagtcatttc tcctactggt gtttgtaaaa
     9001 tttgggtagc aaaaagggaa ggaaggtctc tttctgtaaa ttctcctaaa gagccaccat
     9061 cttctctgct ttcttgatcc atagaatatt cccttgccaa agaagcaaag ctttcacctt
     9121 tggcatagcg attacggatt tcttccatca gttgtctttc gcgtaaagaa tcttcagctg
     9181 taggagtaag ggcttttaag atatggcggg cacggatttc attacctctt ttttcttcta
     9241 agcggataat gtgatagcca tattcacttt ctacgatgtc gcttatttct ccaagctgta
     9301 aagcaaaggc agcatcttca aagggtttaa ccatttgtcc tcttttaaag aaaccgagat
     9361 cacctccaac ttctttactg ggacagtcac tttccgtgct ggcgagggta gcaaagtcct
     9421 caccgttttt caggcgtctt tgaatttctt ttatttctgc cagttttgcc tctttgcttt
     9481 cctggcttgg ttttatttct ctgaaaataa ttcctaaatc ccaggaaacg ggttttacgg
     9541 ctaaggaatc cttggtagca ttatagaaat ttatcacttc tgcttccgta acagagacct
     9601 tgttaattat ttcttttttc aaaatctggt cggaaagtgc ttgttcagtt agtatatctc
     9661 tataaagttg cagtaaatcg gattctgtaa gcttactttt tttaaggtcg gcggcaaaag
     9721 ctgaggaact gggatattga gcttttactt ttttcagata gttctctgcc attgctttta
     9781 tttcatcgtt gttaactgta atattcaggt ctttggcttt ctggataatc aatttctgat
     9841 ttatcatttc atccagaaca tctctgggat ctgtattttc ttccaataaa cctgcacttt
     9901 gcatctgcac catttctttc tgtaaatcgg aaagcagaat aatgtcggtt cctactttgg
     9961 ctactatttt atctatcagt tctgcgctta aagataaagt tagaactaac aggataagga
    10021 ttattgctga ttttcgcatt cttaacttcc ttattagtaa taataaatct cactggtttg
    10081 ggatttgatt tctttaatta attcctgata cacctggtct tgtttttcca gcagaacctt
    10141 gcgtttgatt tctgcccgat aatcttcaaa attagcttcc tgaggtgtaa ttttactttc
    10201 cactatttga aaaacatacc acatcggttc tttataaacc aagccatatt cattttcttt
    10261 caattcgcgt gcttttaacc agaaaagaga atcaggacca gtggcagaaa ccatacccat
    10321 aaatccacct ctgtttttaa gttcttctgt tgaatagcgt tgcacagctt ccgtgaaact
    10381 cattccctgg cttaattgtt gcagaacatt ttccgcagaa atcttatcca ttaaagctat
    10441 acgccggata ttatattcta ctgcattttt ttgaaattct gcctgatgta gacgaaaata
    10501 gttgaagagc tgatcttccg agacctcaat ttcggaaagg cgtttagcaa ttaaagcatt
    10561 ggctttcact tttttgcttg catagtaaat tttttgtttc actgcttgat ctttatttaa
    10621 gccctgttta tcggcataat gagctaaaag agtgaggttt acccaatcct caatatattt
    10681 tttccttatt tcggggctta aattttccca atcctcaacc gaaaaagtgg acttaaagtc
    10741 atccagatag agaatttctt tatctacctg agccaaagca atgccttttt cttctttttt
    10801 agtgcaagca aataacagta taaggattag aggtattaaa gcaaatcttt tcatcttata
    10861 aatagggagg agtgtaagtt attttgatat acggtttttt ctcagccgga gtgctttcat
    10921 tgcaatccca aaattccagt gtgccgaaat ttctgcgttc ctgagtagat tgaatcatta
    10981 ttcctttagg tatcttttct ccggaagtat atgcctgaat tattggagta acatcaaccg
    11041 ttaaagaatc acctttaata aatccaatgc tgtaatctat ataagcaagc ttttcataat
    11101 cgctttttac caaagtaacg ggggtatcta tagagtcctt tacaacatta aaaggataaa
    11161 gagaatagct ggtagaggaa ttgtaatagt cattgttttt tatatgtaga actatttccg
    11221 ctttattcac ggtcagccgt tttagttgta tatctgttaa tgtgttacct tcgttatctt
    11281 taaaaagagt gtaagttggc tcatatttta tataaagacg ggaggaatta agattgctga
    11341 ttttccatag tacggaagga tttgtttccg gtgcttctaa aatataagta tctttagcag
    11401 gagatttatt ataagttatc cattcacctc cagtttccgt tttatatttg aagttaagag
    11461 ttggcttgtt agttccttcc agagaagtta tttctaccca gccctcattt tctgttttaa
    11521 gcacgaaatt ccagccggta gaatcaggat tactccagtt aacaacatca gaggaagaaa
    11581 gattgaaggt aattactttt ccggaactgc ctatggtatc tgcaacagtg tattccgcca
    11641 agaagcttaa atcatcttct gaaatatcgt taaggtttaa ggaatcaagc catacgcagt
    11701 ttaatttgca tagctttaag attaaaggat tacgcggaag agcggaacgc ttgctgatgt
    11761 ttatagttag aaaacagctg tccgtttccg ttatcggaga aggaagctcc gtaaacctta
    11821 aaagacatat tgcaacagcg ttttggtagt taccggcaag cagttttgcc tcttttcctg
    11881 ttatttcttc tttggtttcg gcaggaaaac tgaaacccat aataattcca ggtttatctt
    11941 ctatagttat ggctctaata tcgctgaaat tatcaccggt taagtttttc ttatcagtac
    12001 aaccgcttag cataattacc agaataatta tgcagagtag cagttttaat acctttggaa
    12061 gcaattccat aataataatc ctattttaat taaatctttt atacagtatt ataagtaggc
    12121 gttgtggata gggggatatt tggtcaagct ttttcttttc tctttgcctt gtatgctttt
    12181 ccctcaagta tttccttgtg gataacttgt gtataacttc gttttttatc cttacttatc
    12241 cacagaaaaa gttaccgcaa gcatttatat ataacttatt cacttccacc cacttaattc
    12301 tatccacact tgtggataac ttttttatac aaacgatgtt cttctcgttt tattgtagcc
    12361 atagcttgcc gtagctccgc atcttataat aatttttttg cggaagaaca gcgtcctctg
    12421 tctacacaaa ataataaaaa taataaatcc tgaaaatcca ataaatccag ttatctgctt
    12481 tctatcttca ttattttttc aggtttttaa ttatttcttc cagctgaatt cggaagtcct
    12541 ctttttcccg aaactggttt tccaccatct ttttagcatg gagaaccgta gtatgatctt
    12601 tcctgttata gtattccgct atatctttta aagagagctg aggaattaaa taatttgcca
    12661 gatacattgc tacctgacgg ggaaaagcaa cttgcggttt gcgggttttt tccagtaact
    12721 gggcaggtgt aattccataa cattcacaga ccttttccgt tatagcatct aaagttatag
    12781 aatgcacctt ttccgaaatc atatcggata aaatttccgc tgccagatta atatctatgt
    12841 cttcaggatt aagtttattg taggatgaaa atgccaaaat acgaattaag gaaccttcca
    12901 gagcacgcac actgctggta atgttatcgg caataaaatt aaatattgca tcgcttaaaa
    12961 ccacattttc cggttc
//
LOCUS       DAOPFL010000045        12890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5716_length_12890_cov_112.872614, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000045 DAOPFL010000000
VERSION     DAOPFL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12890
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5716_length_12890_cov_112.872614"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLG69_04560"
     CDS             complement(<1..341)
                     /locus_tag="PLG69_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPI42815.1"
                     /translation="MLINIFSLIPDDLAKSIISRQPDLPEYRIKQVLSWLYKFYLNEP
                     EKMTNLPEEFRAFLKTNYSFFLPEIESKLVSQDGAVKYRLLLEDGEIIESVLIPTEKK
                     NTLCLSTQVGCA"
     gene            complement(335..1174)
                     /gene="ricT"
                     /locus_tag="PLG69_04565"
     CDS             complement(335..1174)
                     /gene="ricT"
                     /locus_tag="PLG69_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPI42816.1"
                     /translation="MSDFIEVIFRTGRIGYYQNTKGLNIQPEDIIIVEVERGDDIAQV
                     IHLGINEEELDAQVLTGKTLSIRRQANSEDLEKMKNLSYEEDKAAKTFLGVLDHYPFE
                     MKLIETIYQFDGNKLTFFFTADGRIDFRNFVRELANIFKTRIELHQTTGRDEARRLGG
                     FGMCGKQYCCGSFLKRFNQVTIKMAKDQNLAGNLTKISGPCGRLLCCLNFEEDFYVEE
                     AKGFPLVGTFVTYQNTKMMVFRLNVLAKKVMLASEEGIIQEIDLDEYSRLPVISVPIL
                     EEC"
     gene            complement(1174..2367)
                     /locus_tag="PLG69_04570"
     CDS             complement(1174..2367)
                     /locus_tag="PLG69_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HPI42817.1"
                     /translation="MQTVIFDDDALSNFYPLTLNRSIGDLRCGITKLRQRLESIFSDS
                     EETCVIIAENLVPLYQERHPDWIINSAVRGEKLYLNSRIIITADAVEQIQKLDFGSCL
                     YTGQNIVAIKVKDTSIFPDVAKLKKKLCNIKLYNCLADIIQDNSRMLMRDYHLHFYDG
                     ENYFETEPGVTVLHPYNVWVGEGAEFSPGVILDASEGPVVIDTGAYIMANAVLCGPVY
                     LGKKSLVKIGAKIYGGTSIGPVCKVGGEVEGTIFQGYSNKQHDGFLGHSYIGEWVNIG
                     ADTNNSDLKNTYKTVAYYSYTHKAKIDSGNQFLGCVIGDHSKTGINCSINTGTVIGIG
                     CNLYGPNLIRDFIPDFSWGEAEKLEKYRFPEFCETAEIVKQRRNLSFTATEKELYKNI
                     YNMRI"
     gene            complement(2348..3517)
                     /locus_tag="PLG69_04575"
     CDS             complement(2348..3517)
                     /locus_tag="PLG69_04575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42818.1"
                     /translation="MYNILFYILCVLVSTGCITYLVLCIRFVYGFKHYKPVFSFKKRT
                     VSVVIAARNEAKNLQDLLLCLLNQDYPSELFEVILADDDSEDETAEVANKYIEAGLNL
                     HYLKITGRENVLSPKKKALEDAVYTAQGEIILSTDADCIVPTTWISSMVSLFTDDVSM
                     VAGYSRTFLPDWKKASLVQKYEHLDYALSYIALAGCYTIGKSWACVGQNLAYRRSAFY
                     DVGGFSKIKHLLSGDDVNLLQLMRNKGYKIIFNFSPSSFVYTQPIQSWKQLINKRSRW
                     TSNMKYQLKFNPEFYFILFSLACLYWGGLIMLFFNYKLALCILAYRIIIENIIFNFSR
                     SRFGITKEIMKFYPVWLIIQSFFLVFTMILGQFNIFVWHGKRPNNKRNNNANRNL"
     gene            complement(3536..5584)
                     /locus_tag="PLG69_04580"
     CDS             complement(3536..5584)
                     /locus_tag="PLG69_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPI42819.1"
                     /translation="MKASTKQKLYSVLKYLWFAFCFITGIFVGALWFYHDSLPPTSEL
                     RNYTLRSGSEVYDRNGKMVYLFAFEKRKLVSLKELPQYLIDALIITEDKHFYHHFGID
                     ILGNIRALAVDIARLDFSQGASTITQQMARNMFLTLDKRLSRKLKEIILALRIEREFT
                     KDEILEIYFNKIFWGGQIHGVETASLYYFGKHARDLTLAESAALVGMIQRPNYYNPAK
                     HPDRLIERRNMILGKMLKAKKITQEEYNEAVASKITSDRSSLQQYSTDYYIEHIRLYL
                     EHKYGTEKLFEGGLKIYTPMDVDLSVYADSVLNRYLSNVEKGYPASMRYDSVPKNSVD
                     INTRYLQGGILLMENSTGQVLAMLGGRNFTHSKFNRITQAKRQPGSAIKPTYYTAAIE
                     KGYTPATVINDAPITLTGGNGKDWTPQNFSRKYYGPTRMRTAITYSYNVWAVKTVMDL
                     GLDVVNDAFRRFGIDAKAEDYSAALGAYEVTPINLISAFTTFPNNGFRTKPVFITKVE
                     DVNGKVLERSSTAKYDVTTPQVAFIMTSLLQSVVTSGTGAAARNNYYWQAAGKTGTSS
                     DHRDAWFIGFNRKYTLGIWNGFDNNAAISASASCAPIWGQIMTKAISNENKGRLPKSD
                     DPRYSFVVPDKIVKRTINPKTGFVSEYGIEEYFIEDNIPPARSDTLSYNYYPTRWGNP
                     "
     gene            complement(5581..6105)
                     /locus_tag="PLG69_04585"
     CDS             complement(5581..6105)
                     /locus_tag="PLG69_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPI42820.1"
                     /translation="MNIHNKSKLTQLAFYTALAASIHIVEDLIMRMLPLPFLRVGFSN
                     IVILYLICHNRIREAFIVNISKTILSGIVTLTLLSPSTLLSLTAGISAIIVMFLVRCL
                     HLGLGIYGISICGAIAHNLTQLAMVRAILIHSDKVFVLTPILLSIALISGSIIAYLTL
                     YIDRKSEKAEIKEK"
     gene            complement(6102..6818)
                     /locus_tag="PLG69_04590"
     CDS             complement(6102..6818)
                     /locus_tag="PLG69_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI42821.1"
                     /translation="MIEVEKLCVSLNDREILVDISFTLNNGNNMVILGRSGSGKTVLI
                     KTLLGIYFPVSGSVVIDGIDIHRSSEDQRKTSKKRFAMVFQNAALLDSFTVLQNVALP
                     LYERGEKDTALIQDRVHKCLQMVGLENTVNLYPAQLSGGMRKRIGIARALVYEPDYII
                     FDEPVSGLDPITSREIMYYITRIVETASATLITITHELKDIRTIANQVLFLNEGRVIF
                     NGKTSDLYQSHNPLVQEYLQ"
     gene            complement(6805..7161)
                     /locus_tag="PLG69_04595"
     CDS             complement(6805..7161)
                     /locus_tag="PLG69_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPI42822.1"
                     /translation="MKFFRTLYPTLIWFCLIWILSSLPSQHIPSVNIIGFDKLEHIGI
                     YAILGCLLGYWLIFKDWKLTTVILIYVFLLLLAGLDEYHQNFIPGRDVSLYDFMANSA
                     GIIIGFLFYLHKYDRS"
     gene            complement(7158..7823)
                     /locus_tag="PLG69_04600"
     CDS             complement(7158..7823)
                     /locus_tag="PLG69_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPI42823.1"
                     /translation="MLIASTAEYYMKALGIKLAEISIQISENEPVIEIKAKSLKETAI
                     SPQLNNIYRIEHNGSYQPISYTRVIRQKNVNDKVIVNYNHNSSQATMIRLSDNSTIQY
                     SIAKNSQDVYSFLAEVIAGRVQTGNYPIDANGVKWQANVVQLASETVDTPLGKYPARH
                     YEITFHNLTEKKMPYIDMVTFNMLQENNKLNLWVYNNQYAVKATFKKKGLSSCWELVN
                     IKK"
     gene            complement(7859..9124)
                     /locus_tag="PLG69_04605"
     CDS             complement(7859..9124)
                     /locus_tag="PLG69_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPI42824.1"
                     /translation="MDDGKAFIIIVLFALSAFFSGSETALFSLSRIQLKRLENNDKSS
                     AKRIIKLLSKPRQLLITLLLGNTFVNLAISSFGTLLALQIGTKYGYTESSVVTVQIII
                     TTILILFLGEMTPKLIALSKADSFSKIISLPLMGFRLIFFPVVWVLEKISELISKRKA
                     VDQYIGTQFTHEEFHNLLQSESSSHSLEEHEKKMLVGLFRFKEAEIKEIYVPRVKITA
                     IEENKDLEELKQIIIDSGYSRIPVYRGSIDEIVGIIYVKDLLLYPEKKTIKELMRPVW
                     FVTENMKVQALLNQFKQKKLQIAVVVDEYGGTSGIISLEDILEEIVGDIRDEYDKDEI
                     PELIKKDENTYIISGNFSVRQFNEDFSTEISVEDYDNIAEFLLAYFNNVPAIGATAKY
                     DDKLEFTILDADEKSIKQIQVKIKPGELS"
     gene            complement(9127..9591)
                     /gene="ybeY"
                     /locus_tag="PLG69_04610"
     CDS             complement(9127..9591)
                     /gene="ybeY"
                     /locus_tag="PLG69_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPI42825.1"
                     /translation="MNSLELIGETIIPESEQEKIAELICSREDPEHSYQICVKTVDSE
                     TMKHYNKRYRGLNETTDILSFITADLPACAIEERDEEDGFSAKPVRICDIIIDIKRLF
                     QQKGIRTLEEEYRAVLIHGLLHLVGYDHIRSADAEKMNKKEEYYLKQTQGEI"
     gene            complement(9584..11680)
                     /locus_tag="PLG69_04615"
     CDS             complement(9584..11680)
                     /locus_tag="PLG69_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPI42826.1"
                     /translation="MNSKYILYCIVTALIIVGIYQIVAVGRVNYPEYNLKEGQVSEVE
                     IIAPFDFPVLKSPQELAEEKEQVLSQVAKPYEISEEPLFEAISNLDNIYAVLCSIEPT
                     DDWEKIASDLKRAGYSFSPEALEFTLRSSLRDRIYESLRRELGTLYNQGIYESLEEDS
                     ILIWQNDNAEKRALSDFLPREKAKEILIDKFPEAKTFVSEFTEQYLKPNLLVNEEKLN
                     ELNQSSLQTVQPSEGMVLKNEVIVRKNSRVTEEEVRKLESLQEAYKRQKIRKSPLQEM
                     FLSLGLLMFIFVVIFLANHYYGVQSKEEKIPVADFLPVNLGFIVLVILTVLSNFVLSG
                     SNLIIPFAMIAIAGAILVNFEFGLLYSVCNILVISPFLNWETFTPVIMLLSTIMCLLI
                     LRRQKVWHEYLMIWLYLLISLLVVIICLGIYKRDSIITIMRSIGYAFISASLSITGIL
                     LIVPYYEKKWNRATKQTLLELLDFNHPLLKRLATEAVGTYHHSLVVGNLAEHAAEAIG
                     ANPLLARVGSYYHDIGKIINPEIFTENNEDSSEIHSQIEPNESAQLIRNHVTEGITLA
                     KKYKIPQPVIDIIMQHHGDMIIRYFYEKANKEGKELDANAYRYPGPRPQTKEAALVML
                     ADIVESTTKAKEIESETDIAKIIDDTITYLLKEKQFDEAPISMKDLKIVKQSFIPVLE
                     SIYRKRLDYPEAQKDE"
     gene            12316..12789
                     /locus_tag="PLG69_04620"
     CDS             12316..12789
                     /locus_tag="PLG69_04620"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPI42827.1"
                     /translation="MIQLSELPVSYGKIHLPAKVALVVTQKPESGYNLITIEWFMRTS
                     LQPPMFAISIGKTRYSHECLEANRYFNLVFPSLKMKPLLTLCGSNSGRDMDKFAVGKV
                     EYIAGKLHKLPVLKDAVACFECEIVSQIRSGDHTIYIGEVHYCWQNPEEELFYYQ"
BASE COUNT         4019 a         2521 c         2227 g         4123 t
ORIGIN      
        1 gcacaaccaa cctgagtaga aaggcaaagt gtattcttct tctctgtagg aattaaaaca
       61 ctttcaatta tttcgccatc ctctaaaagc aaacgatact ttacagctcc gtcttgggac
      121 accagtttgc tttctatttc aggtaaaaag aaggaataat tggtctttaa aaatgccctg
      181 aattcctcag gcagattagt cattttttcc ggttcattga gataaaactt atataaccag
      241 gaaagaacct gcttaattct gtattcaggt aaatcgggtt gcctgctaat aatgcttttt
      301 gccaggtcat ccggtattaa agaaaaaata ttgattagca ttcttccagg atgggaacac
      361 taataaccgg aagacgggaa tattcatcta aatcaatttc ttgaattatt ccttcctcac
      421 tggcaagcat cacttttttg gctaaaacat taagacggaa aaccatcatt tttgtattct
      481 gataggtaac aaaggtgcca actaaaggaa accccttggc ttcttccaca taaaaatcct
      541 cttcaaaatt taaacagcaa agcaaacgcc cgcaaggtcc ggaaattttg gttaaattac
      601 ctgccagatt ttggtcttta gccattttga tggtaacctg attgaagcgt tttaaaaaac
      661 tgccacaaca atattgtttc ccacacattc caaaaccacc taaacgacgt gcttcatctc
      721 tacccgttgt ttgatgaagt tcaatgcggg ttttaaaaat attagccaat tcccgaacaa
      781 agtttctaaa atcaattctg ccatcggcag tgaagaaaaa ggttaattta ttaccgtcaa
      841 actggtaaat tgtttcaatc agtttcattt caaaagggta atgatccaga acacccagaa
      901 aggtcttagc ggctttatcc tcttcataac tgagattttt cattttttcc aggtcttccg
      961 aatttgcctg gcgtcggata cttaatgttt ttcctgttaa aacctgagca tccaattctt
     1021 cttcattgat gcccagatga attacctgag ctatatcatc acctctttcc acttctacta
     1081 taataatgtc ttcgggttga atattcaagc ccttggtatt ttggtaataa ccaattcgtc
     1141 ccgttctaaa tatcacttct ataaagtccg acattatatc ctcatattat atatattttt
     1201 atacagttct ttttcagttg cggtgaagct taaattacgc cgctgtttaa ctatttctgc
     1261 cgtttcacaa aactccggga aacggtattt ttctaatttt tctgcttctc cccaggaaaa
     1321 atctggaata aaatcacgaa ttaaattcgg tccgtaaaga ttgcacccaa tgccgattac
     1381 agttccggta ttaatactgc aattaatacc tgttttgctg tggtctccaa ttacgcaacc
     1441 taaaaattga tttccggagt caattttagc tttatgggta taggaataat aagctacggt
     1501 cttgtaagta tttttcaggt cactattatt ggtatcggca ccgatattta cccattcacc
     1561 gatataggaa tgccccaaaa aaccgtcatg ttgcttatta ctataaccct gaaaaattgt
     1621 accttcaacc tctccaccca ctttacaaac gggtccgatg gaagtgcctc cgtaaatttt
     1681 agcaccaatc ttaacgaggg attttttacc taaataaaca ggaccgcaaa gaactgcatt
     1741 agccattata tatgctcccg tatcaattac aacaggtcct tccgaagcat ccaaaattac
     1801 acccggtgaa aattccgctc cttcgcccac ccaaacatta taaggatgca aaacagtaac
     1861 tcccggctcg gtttcaaagt aattttctcc atcataaaaa tggagatgat aatcacgcat
     1921 aagcattctg gaattatctt gaatgatatc tgcaaggcag ttgtagagtt taatgttgca
     1981 gagttttttc ttcagtttag ctacatcagg aaaaatagaa gtatccttca cctttatagc
     2041 tacaatgttc tgtcccgtat ataaacagga accgaaatca agtttttgta tttgttccac
     2101 agcatcagca gtaataataa tccgggaatt caaataaagt ttttctcctc ttacggcaga
     2161 attgattatc caatcgggat gcctttcttg atataacgga acaagatttt cggctataat
     2221 tacacaggtc tcttccgaat cggaaaaaat actctctaac cgttgacgca atttagttat
     2281 tccacaacgc aggtctccaa tacttctgtt taaagttaaa ggataaaaat tggatagtgc
     2341 atcatcatca aagattacgg tttgcattgt tattcctttt attgttgggt cttttgccgt
     2401 gccacacaaa aatattgaac tgacccagta tcattgtaaa caccagaaaa aaactctgaa
     2461 taattagcca tactggataa aacttcatta tttctttcgt aattccaaaa cgcgaacggg
     2521 aaaaattgaa aatgatgttt tctataataa tgcggtaagc caaaatacac aaagctaatt
     2581 tataattaaa aaacagcatt atcaacccgc cccaatataa acaggcaagc gaaaacagaa
     2641 taaaatagaa ctcaggattg aacttcagct ggtatttcat attggaagtc caacgactgc
     2701 gtttattgat tagttgtttc caggattgaa tcggctgagt ataaacaaaa ctggaaggtg
     2761 agaaattgaa aataatttta taacccttat tacgcattaa ctgcagtaaa tttacatcat
     2821 ccccggaaag caaatgtttt attttactaa aaccccctac atcataaaaa gcagaacgtc
     2881 tgtaagctaa attttgtcct acacatgccc aacttttacc aattgtataa caacccgcta
     2941 aagctatata ggataaagca taatccaaat gttcatattt ttgcacgaga gatgccttct
     3001 tccaatcagg aagaaaagtt ctggaataac ctgcaaccat agaaacatca tctgtaaata
     3061 gagaaaccat agaagaaatc caggttgtcg gaacaataca atctgcatca gttgaaagaa
     3121 taatttctcc ctgggcagta taaacagcat cttccagagc tttctttttg ggggaaagga
     3181 cattttcccg tcccgtaatt ttcaaataat gtaaatttag acctgcttca atatacttgt
     3241 ttgctacctc cgcggtttca tcttcagaat catcatcggc taatataacc tcaaaaagct
     3301 ctgagggata atcctgattg agcaaacaca gcaataaatc ctgtaagttc ttagcttcat
     3361 tacgggcagc aataactaca ctcactgtcc tttttttaaa actgaaaacg ggtttatagt
     3421 gcttaaaacc atacacgaaa cggatacata aaacaagata ggttatgcaa cccgttgaaa
     3481 caagaacaca gagaatataa aaaagaatgt tatacactct taactttcct tcttattagg
     3541 gatttcccca acgggtggga taataattat agcttaaagt atcggaacgg gcaggcggaa
     3601 tattatcctc aataaagtat tcttcaatgc catattccga aacaaaaccg gttttaggat
     3661 tgatagttct cttcacaatt ttatccggaa ctacaaaact atagcgggga tcatcacttt
     3721 taggcaggcg tcccttattt tcgttactaa ttgccttagt cataatttga ccccaaatag
     3781 gagcacaact tgcactggca gaaatggcag cattgttatc aaagccgttc caaatgccta
     3841 aagtatattt acggttaaag ccaataaacc aagcatcacg atgatcactg gaagttcctg
     3901 tttttcctgc tgcttgccaa taataattgt ttctggcagc agctcccgtt ccggaagtga
     3961 ctactgattg cagcaaagag gtcattataa aagcaacttg gggagtggta acatcatatt
     4021 ttgcagtact actgcgttcc agaacttttc cattaacatc ttccactttg gtaataaaca
     4081 cgggttttgt gcggaaaccg ttattgggaa aagttgtgaa agcggaaatc agattgatag
     4141 gggtaacttc ataagcgcca agagcagctg aatagtcctc tgctttggca tcaattccaa
     4201 aacgccgaaa agcatcatta acaacatcca gtccgaggtc cataacggtt ttaacagccc
     4261 aaacattgta agaataggtg atggcagttc gcattcttgt aggtccgtaa tatttacggc
     4321 tgaaattttg aggagtccaa tcctttccat tgccccctgt taaagttata ggtgcatcgt
     4381 taataactgt agcgggagta tatccctttt caattgctgc cgtataataa gttggtttaa
     4441 tagcacttcc tggttgtctt tttgcctgag taatgcggtt aaatttactg tgagtgaaat
     4501 ttctgcctcc aagcatcgct aaaacctgtc cggtactatt ttccattaat aggatacccc
     4561 cttgcagata acgggtattg atatccacac tgtttttagg aacactgtca taacgcatag
     4621 aggcaggata acctttttca acattggaaa gatagcgatt caaaacagaa tcggcataaa
     4681 cggaaaggtc aacatccatt ggagtgtaaa tctttaatcc gccttcaaaa agtttttccg
     4741 tgccatattt gtgttccaga tacaaacgaa tatgttcaat ataataatca gttgaatatt
     4801 gttgcagaga actgcggtca cttgttattt tggaagcgac tgcctcatta tattcttcct
     4861 gggttatttt ttttgccttc agcatctttc ctaaaatcat attccttctt tcaatcagac
     4921 ggtcggggtg tttcgcgggg ttataataat tgggacgttg aatcattccc actaatgcgg
     4981 ctgattctgc caaggttaag tcgcgtgcat gtttaccaaa atagtataaa gaagctgttt
     5041 ccacaccgtg aatttgtcca ccccaaaaga ttttattgaa ataaatctcc aggatttcat
     5101 ccttggtaaa ttcccgttca attcttagtg ctaaaataat ttccttcagt ttgcgtgaaa
     5161 ggcgtttatc taaagtaaga aacatattgc gtgccatttg ttgtgtgata gtgcttgcac
     5221 cttgcgaaaa atccagacgc gctatatcta cagccaaagc acggatatta cctaaaatat
     5281 caattccgaa atgatgatag aaatgtttgt cttccgtaat tattagagca tctatcagat
     5341 attggggaag ttcttttagg gaaaccagct tgcgtttttc aaaggcaaaa agataaacca
     5401 ttttaccatt tcggtcataa acctcagaac cggaacggag agtataattt ctcagttcac
     5461 tggtgggagg taaactatcg tggtaaaacc ataaagcgcc tacaaaaata cctgtgatga
     5521 agcaaaaggc aaaccacaga tatttcagga cgctatataa tttctgttta gtgctggctt
     5581 tcatttttct ttaatttcgg ctttttcgct tttcctgtct atatataaag ttaagtaagc
     5641 aattatgcta ccgctaatca aggcaattga gagcaggatt ggtgtcaata caaaaacttt
     5701 atcactgtga attaaaatgg cacgaaccat agctaactgt gtaagattat gggcaatagc
     5761 tccacaaata ctgatgccgt aaatcccaag tcccaaatgc agacaacgta ctaaaaacat
     5821 aacaataata gcagaaatac ctgcagttaa ggataacaaa gtagaaggac tgagcaaagt
     5881 gagggtaaca attccgctta atatggtttt actgatgtta actataaatg cttctctaat
     5941 tctattgtga cagataagat agagaataac gatattggaa aaaccgactc gcagaaaagg
     6001 aaggggcaac attcgcatta taaggtcttc cacaatatga atagaagctg ctaaagcggt
     6061 gtaaaaagcc agttgagtga gttttgattt attgtggata ttcattgtaa atattcttgc
     6121 acgaggggat tatgtgattg gtataagtca cttgtttttc cgttgaaaat aaccctgcct
     6181 tcattcaaaa ataaaacctg attggcaatt gtccgaatat ctttcagttc atgggttatg
     6241 gttatcaaag tagcggaggc agtttccaca atgcgggtga tgtaatacat aatttctcgg
     6301 gaggtaatag ggtccagtcc agaaacaggt tcatcaaaaa taatataatc cggttcataa
     6361 accaaagctc tggcaatgcc aattcttttg cgcataccac cactaagctg ggcaggatat
     6421 aggtttacgg tattttccag tccaaccatt tgcaggcatt tatgaaccct atcttgaatt
     6481 aaagcggtat ctttttctcc gcgttcataa agtggcaaag caacattttg cagaacagta
     6541 aaggaatcaa gtaaagcagc attttggaaa accatcgcaa agcgtttttt agatgttttc
     6601 ctttggtctt ccgaagagcg gtgaatatct attccatcaa taactacact gccggaaaca
     6661 ggaaaataaa tgcctagcag ggttttgatt aaaacagttt ttccgctacc gctacgaccc
     6721 aaaataacca tattgttgcc attattaagc gtgaaactga tatccaccaa aatttctcta
     6781 tcattcagag aaacgcagag cttttcaact tctatcatat ttatgcaagt aaaataaaaa
     6841 gccgattatg attccggcag aatttgccat aaaatcatat agggaaacat cgcgcccagg
     6901 aataaaattc tgatggtatt catccaaacc ggcaagcaac aaaagaaaca cataaattag
     6961 gatgacagtt gttaatttcc agtccttaaa aattagccag tatccaagca aacagcccaa
     7021 aatagcataa atacctatat gttccagttt atcaaaaccg ataatattta cggaaggaat
     7081 atgttgagag ggcagagatg ataaaatcca gatgaggcaa aaccaaataa gagtagggta
     7141 aagagtccga aagaatttca ttttttgata tttaccaatt cccaacagga agagagtcct
     7201 ttctttttaa aagttgcttt cacggcgtat tgattgttat atacccacag gttcagttta
     7261 ttattttcct gcagcatatt gaaggttacc atatctatat agggcatctt tttctcggtt
     7321 aaattatgga aagtaatttc ataatgtctt gcaggatatt taccaagcgg tgtatcaact
     7381 gtttcagaag cgagctgaac aacatttgcc tgccatttta caccgtttgc atcaatagga
     7441 taatttcccg tttgcactct tccggctata acttctgcca gaaaagaata gacatcctgg
     7501 ctgtttttag ctatagaata ttggatagtg ctattatccg ataagcgaat catagttgct
     7561 tgagagctat tatgattgta gtttacaata actttatcat taacattttt ttgacggata
     7621 acccgggtat agcttatggg ctgataggaa ccgttatgtt caatccgata aatgttgttt
     7681 aattgagggg atatagctgt ttctttcagg gatttggctt ttatttctat aaccggttca
     7741 ttttcactaa tttggatact tatttcagcc agtttaattc ctaatgcttt catataatat
     7801 tctgcagttg atgcaattag caaactgtgc cagagtaaaa atagggttaa gaggtatttc
     7861 aggacaattc tcccggcttg attttcacct gaatttgttt gatgcttttc tcatctgcat
     7921 ccaaaatggt aaattccagt ttatcatcat atttggcagt tgcgccaata gcaggaacat
     7981 tattgaaata agcaagcaga aattcagcta tattatcata gtcctccacc gagatttccg
     8041 tagaaaaatc ctcattaaac tgtcggacgc tgaaatttcc gcttataata taggtatttt
     8101 catctttttt aatcagttcc ggaatttcat ctttatcata ttcatctctg atatcgccaa
     8161 ctatttcttc cagaatatct tccaaagaaa ttataccgct tgtgccacca tattcatcaa
     8221 ccacaactgc aatctgtaat tttttctgtt tgaactgatt tagcaatgcc tgcactttca
     8281 tattttccgt cacgaaccat acgggacgca tcaattcttt aatagttttc ttttcgggat
     8341 acaagagcag gtcttttaca taaattattc ctacaatttc atctatagag ccgcgataaa
     8401 cgggaattcg ggaataaccg ctgtcaataa taatctgttt cagttcttca aggtctttgt
     8461 tttcttctat agcagttatt tttactcgtg gcacataaat ttcttttatt tccgcttctt
     8521 taaatctgaa aagacctacc agcattttct tttcgtgttc ttccaagctg tgactgctgc
     8581 tttcggattg cagcagatta tgaaattcct catgggtaaa ttgcgttcct atatattggt
     8641 ctacagcttt ccttttggat ataagttcgc ttattttttc caaaacccat acaaccggga
     8701 aaaatattaa ccgaaatccc atcagtggca gggaaataat tttactaaaa ctatctgctt
     8761 tggaaagagc aatcagtttg ggagtcattt ctcctaaaaa caaaatcaga atggtagtaa
     8821 ttataatttg gactgttact accgatgatt ccgtataacc gtatttagtt ccgatttgta
     8881 aagccagcaa agttccgaag gaagaaattg ccaagtttac aaaagtattg cctaaaagca
     8941 aagttatcag taactgtctg ggtttgctca gtaattttat aatccttttt gcactgcttt
     9001 tatcgttatt ttccaagcgt ttcagctgaa tgcgtgaaag cgaaaaaaga gccgtttccg
     9061 agccggaaaa aaaagctgat aacgcaaaaa ggacaattat tataaatgcc ttaccgtcat
     9121 ccactgctat atttcacctt gtgtctgttt taaataatat tcttcttttt tgttcatttt
     9181 ttctgcatct gcactgcgga tatggtcgta accaactaaa tgtaacagac catgtatcaa
     9241 aacagcgcga tattcttctt ctaaagttct aattcccttt tgctgaaaaa gccgttttat
     9301 gtcaataata atatcacaaa tacgcaccgg tttggcggaa aatccatctt cctcatccct
     9361 ttcctcaatt gcacaagcgg gtaaatctgc agtaataaaa cttaaaatat cggtggtttc
     9421 atttaaaccc cgatagcgtt tattgtaatg tttcatcgtt tcactatcta ctgttttaac
     9481 gcatatctga taactatgtt cagggtcttc ccgagagcat atcagttctg caattttttc
     9541 ctgttcggat tccggtatta ttgtttcgcc gattaattcc aaactattca tctttttgtg
     9601 cttccggata gtccagtcgt ttcctataaa tactttccaa aactggtata aagctctgtt
     9661 tcactatttt caggtctttc atagaaatcg gtgcttcatc aaactgtttt tctttcaata
     9721 aataagttat ggtatcatca ataattttag cgatatctgt ttcgctttca atttctttgg
     9781 ctttagtagt ggattccaca atatctgcca gcatcactaa agctgcttct ttggtttgag
     9841 gacgaggacc aggataccta taggcatttg catccagttc ttttccttct ttatttgcct
     9901 tttcataaaa atagcgaatt atcatatcgc catgatgttg cattataata tctattaccg
     9961 gttgaggaat tttgtatttt tttgccaaag taataccttc tgtaacatga ttacgaatca
    10021 actgagcact ttcattcggt tcaatctggg aatgaatttc gctggaatct tcattatttt
    10081 ccgtaaatat ttcgggattg attatttttc ctatatcgtg ataataactt cctactctgg
    10141 ctaaaagagg attagctcca atggcttcag cggcatgttc tgccaaattt cccactacta
    10201 aagaatgatg ataagttcca actgcctcag ttgccagtcg tttaagcagg ggatggttaa
    10261 aatccaaaag ttccaatagg gtctgttttg ttgccctgtt ccactttttt tcataataag
    10321 ggacaatcaa taaaataccg gttatactta aagatgccga aataaaagca taacctattg
    10381 aacgcattat agttattatg gaatctctct tgtaaattcc caggcatata attacaacta
    10441 acaaagaaat aagtaaatat aaccaaatca tcagatattc atgccacacc ttttgtcgcc
    10501 gtaaaattaa cagacacatt atggtagata ataacattat taccggagtg aatgtttccc
    10561 aattcaggaa aggacttatt accaatatat tacaaacact gtaaaggagt ccgaattcaa
    10621 aattgactaa aatcgctccc gctatggcaa tcattgcaaa aggaattatc agattactgc
    10681 cggaaagaac aaaattgctg agcaccgtta aaataaccag cacaataaaa cctaaattta
    10741 caggcagaaa atccgctacc ggtatctttt cttctttgct ttgaactccg taatagtgat
    10801 ttgccagaaa gataaccacg aagataaaca tcagcaaacc gagggataaa aacatttcct
    10861 gcagaggcga tttgcgtatt ttttgacgct tatatgcctc ttgcaaggat tccagtttcc
    10921 taacttcttc ctctgtaacg cgggaatttt tgcggacaat aacttcgttt tttaagacca
    10981 ttccttcgct tggctgaacc gtttgcaggg aactttgatt cagttcattc agcttttctt
    11041 cgtttacaag cagattgggc ttgagatact gttccgtaaa ttcgcttaca aaggtttttg
    11101 cttcgggaaa tttatcaatc agtatctctt ttgctttttc ccggggaaga aaatcactaa
    11161 gcgctctttt ttccgcgttg tcattttgcc aaatgaggat actatcttct tccagcgatt
    11221 cataaatccc ttgattgtat aaagttccca gttcacgccg cagagattcg taaattctat
    11281 cccgcaaaga tgaacgcaga gtaaattcaa gtgcttcggg agaaaaggaa taaccggcgc
    11341 gttttaagtc cgaagcaatt ttttcccaat cgtcagttgg ctctattgaa cataaaacag
    11401 cataaatatt atccagattg ctaattgctt caaaaagcgg ctcttccgaa atttcgtagg
    11461 gcttagcaac ttgggaaagc acctgctctt tttcttcagc cagctcttga ggcgatttga
    11521 gaacaggaaa atcaaaggga gcaattatct ccacttccga aacctgtcct tctttcaaat
    11581 tatattcagg gtaattaacc cgtccaacgg caactatctg gtaaatgcca acgattatta
    11641 aagccgttac tatgcaataa agtatgtatt tagaattcat aattttaact tttttacaat
    11701 atagcttctc tgtcaagtgt catttttcct ttgtgtttat ttttgctttc tgcctgcttt
    11761 ctcttttatt taataaatat gaaacaatta ggtaaagatg cagtagtttt attacggtaa
    11821 tgttacttga gtgttacttt agtgttacgg tagtgttagg gaatcaatta ggaaaggact
    11881 ataaataagt aagttaaatg cctacttttt tacctctccc ggataacaca tatttcacag
    11941 ataaaaatgc acacggactt cgtatctttc gtagcttaaa tagtacacgg attgcgctga
    12001 tttcgtagat tttttatttg ataagccatt aacttcaaac ttgtgatgac tttttttgta
    12061 gttggaagat gtcgttcttc cgcaagaaaa aattttttta attagcggag ctccgtctac
    12121 acaatttaat ttcggaggtt ttttatggct gtcaacatac cgaaaatcta aaccttaaca
    12181 accctccaaa ctttctaaac cctttttctc ttttctcttt ttctcattgt gaaaataggt
    12241 tctttttttt tgtctttgta agaagtaaaa tttggaatat tatatcaata aagacatttg
    12301 aactaaggag aaactatgat tcagctctca gagctacctg taagttatgg taaaatccat
    12361 cttccggcaa aagtagcatt ggttgtaacc caaaaacccg aaagcggata taatttaatt
    12421 accattgaat ggtttatgag aacttctcta caaccaccga tgtttgcaat ttcaatcggc
    12481 aaaacaagat actctcacga atgtttggaa gcaaacagat actttaattt agttttccct
    12541 tctctgaaaa tgaaacctct tttaacttta tgcggttcca attcaggcag ggatatggat
    12601 aaatttgccg tgggcaaagt ggaatatatt gcaggtaaat tgcataaact tccggttttg
    12661 aaagatgccg ttgcctgttt tgaatgcgaa attgtttccc aaattcgcag cggagatcat
    12721 acaatttata ttggagaagt gcattattgc tggcaaaatc ctgaggaaga actgttctat
    12781 tatcagtaag gatattaaac ccaaacactt caggaaatga agaatatata aaataagaaa
    12841 accaggaaaa acttggtaat ttaaggttta tttctttgca aaagcccgaa
//
LOCUS       DAOPFL010000046        12556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_5936_length_12556_cov_125.245660, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000046 DAOPFL010000000
VERSION     DAOPFL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5936_length_12556_cov_125.245660"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..308)
                     /locus_tag="PLG69_04625"
     CDS             complement(45..308)
                     /locus_tag="PLG69_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPI42828.1"
                     /translation="MKQVEVTVTNKLGLHARPSALLVKAATKYRSEFFIEKEGMRVNG
                     KSIMGVMMLAAECGSKLQLIANGVDEDYLLAEIKEMIENGFGE"
     gene            complement(277..1266)
                     /gene="hprK"
                     /locus_tag="PLG69_04630"
     CDS             complement(277..1266)
                     /gene="hprK"
                     /locus_tag="PLG69_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPI42829.1"
                     /translation="MKEITVNDFFDAKKKDLALSLITEPETLNKKIGSPHINRPGLAL
                     AGYMEIFAADRIQILGETEVRYLQSLPEEVLISRIREMFKMDIPCIIVTKGLTLPPVI
                     EYLANELNIALLSSRLSTINLIDNLSRYLREIFVLEKTIHATLVDVFGLGILITGKSG
                     IGKSECALDLVHRGHSLVGDDLITLRYIDDQLIGKAGRDIGHFMEIRGVGFVNAERVF
                     GIAHTRKQKTIDLQIELMPWQENMDYERIGLVNNYAEHLGVKIPIIYLPVSPGKNVSV
                     IVEVAAMNVILKSVGYDAAEDFNRKINDEIRKKTMQKNFEGKGDETGRSDSNQ"
     gene            complement(1484..2020)
                     /gene="raiA"
                     /locus_tag="PLG69_04635"
     CDS             complement(1484..2020)
                     /gene="raiA"
                     /locus_tag="PLG69_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPI42830.1"
                     /translation="MQITITARHFELTKAIRDYVETACSKLNKYFDQIITIHTVLALE
                     NSRNICELSLHAAKFSLQSEAEEMDMYLAIDNAVEKMEGQIKKLKDRITDHQKRALKQ
                     QFETYSYATDFQLDAKNHSRKTIKTKKVVTESLSLSEALEKLEASKEDFLIFKNIESD
                     KINVLVTKDHELYKLLEP"
     gene            complement(2036..3442)
                     /gene="lpdA"
                     /locus_tag="PLG69_04640"
     CDS             complement(2036..3442)
                     /gene="lpdA"
                     /locus_tag="PLG69_04640"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPI42831.1"
                     /translation="MQHCQVIVIGGGPGGYESAIRLSQYGISSLLMEKERLGGLCLNW
                     GCIPTKALVKSAELYSEMLNADNFGLPKPEITLAYNKVWERKNAVVEQLASGIEFLFS
                     KRDIPVIKANAVSVTKEKGNYIVTTLEGESYSSEYVILATGSETKELPGIKIDEKDIL
                     SSTGILKMQELPKELAIIGGGVIGCEFASIFSAFGVQVTIIEFLPHLIATEEEEISKR
                     LAMQFKKNGVKVITNTGVQEIIKKDKRLELKLNNNTTLTADKVLLSVGRKPVNNLTWK
                     GININEEKGAIVINDFMQTSCDKIYAIGDVTGKMQLAHTASKQGLMAAQHIASRLYPE
                     KYFAPQEPLNYNNIPRCIFTHPEIASVGYTQKDAEEIFGDVIVGKFPFAANGKAVAMG
                     NINGFVKTIARKDTSELVGMHIMGPNAAELIAQGTIMIVNKNKAKDIDSFVFAHPTLS
                     EAIMEAMEDLQSKSIHKI"
     gene            complement(3443..4900)
                     /gene="rpoN"
                     /locus_tag="PLG69_04645"
     CDS             complement(3443..4900)
                     /gene="rpoN"
                     /locus_tag="PLG69_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPI42832.1"
                     /translation="MSAMEQYLSLKQKQELALKPKMLQSLKMLALPILELESYIKQEL
                     VNNPLLELREEKDEDDIDESNFEQSTPESSTPIEESDMSENHLETLAEAEELTEILDT
                     WNEYHSSYEGWSSDSETDHTEALIRYEENGKEKFLEQLFPLELPENEMDFITDVVDSC
                     DSYGFLPKGFNIVNLAKKYLITAERAEELHNIILSLSPKGISARNITECLMAQLTEEQ
                     KQNRILVGLVSEHFENLIHRRYQKIASAYGVSEETILLAKEQISKLDPKPGLRILSTS
                     AVYVFPDVTMKLIDGKYEIIINDHITPNIIISPRYRKMINRGYFDKETMQYVRERINS
                     AKFLIKSIYMRMRTLERVSLSIINHQYDFFYLGTGQMKPLTYAVIARDLGVNESTISR
                     VVKHKYAETPYGIYAFKDFFTSTAGFDDDYQSVSRQKVKTHLIRLIEMEDKKKPYSDQ
                     ELVELLKAEGLNISRRIITKYRDELGILNSHLRRQ"
     gene            complement(4897..5682)
                     /gene="lptB"
                     /locus_tag="PLG69_04650"
     CDS             complement(4897..5682)
                     /gene="lptB"
                     /locus_tag="PLG69_04650"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPI42833.1"
                     /translation="MTKKQLSLSYYFMEKHKIRAENLVKIYGKRAVVNDLSLEMSQGE
                     VVGILGPNGAGKTTTFYMIIGLAKPNNGKVFYDDMDISHKPMYKRARLGLGYLAQAPS
                     IFAKLTVEENILAILQTLKISRKEQKNRLAEALEELGLSPLAKQKAYTLSGGERRKLE
                     ITRALVTNPTFLFMDEPFAGIDPIAVADIQDIIAKLRNKDIGIMVTDHNVLETLKIVN
                     RAYIIYEGKIIVSGSSLELINDEKAKELYLGERFLESPFEQRL"
     gene            complement(6062..6823)
                     /locus_tag="PLG69_04655"
     CDS             complement(6062..6823)
                     /locus_tag="PLG69_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42834.1"
                     /translation="MVRGLTTFREHFIDYKEQYVLIGGAASSEILSQSGLTFRATRDL
                     DIVLIVEALSAEFGRAFWEFIEKGNYRSIFQNKEEKKCYRFNNPVNAMYPEQIELFSR
                     IPETIDFKGYGRITPIPIDNELSSLSAIILDDNYYSLIKNGLMDINGLSCLRPAYIII
                     LKAKAYLNMTKNIAEGENITSQKLNKHKNDVFRLFPVLEPNEIIELPLSVKNDLRDFI
                     FLIENDTTINIKNLGIRRIGLKEVIERLKIIFNLD"
     gene            complement(6817..7782)
                     /locus_tag="PLG69_04660"
     CDS             complement(6817..7782)
                     /locus_tag="PLG69_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42835.1"
                     /translation="MKNIALKTKEYLENMLDVKVEIRHSNIGYKLPHFFSEKYIFYDL
                     TLNGKHYLLIEGKDMLTAKVVKLHSHILENRFGIKVIYLSKSINPSLRRAFIKERISF
                     IIPDNQIYLPHLGILFNESCPQEVKKRNRFSPLTQVVIIRTLLEKEYQLSTVSMYAKK
                     YYLNVMYVSRAFRELTEYELITRDECGKEKPIHWLFTGKELWEKAQPYFFNPINKVIW
                     FRLNRQVPFCLAGFSALSKYSMLTPENYESYATTRIIAKKFPDLVEIPYKEEQTNELQ
                     LWRYNPKLITSEDYVDPLSLFLCFKEESDERVQMALEEMMEKIKW"
     gene            complement(8201..9280)
                     /locus_tag="PLG69_04665"
     CDS             complement(8201..9280)
                     /locus_tag="PLG69_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HPI42836.1"
                     /translation="MVKKGFVFFLLFLLLLPLFGKKKGEEEFRLVHSDKLFMTKMENE
                     QALELNGNVHFFYGEVEFLCRNALILDKQKIARLSGNVIVKNDSLTLEADTLAYYRIP
                     DLMNLGGRITATERTKEGIYRWIKSNFATYDKKNDILTTWSRVHGFDYQENARVKCGY
                     AQWDRGKGYALLLDEPYITSGIEDTLAVAAEKMEYFEPERKLIATFNVQVDRGEFHTT
                     SDFLIYFLEEEKAIFTGEPKFISDFADAKATEFHLFMKERKLVKAELIDSCHIDFAEE
                     RFKEKKNTVDADIVTMDFLDEQLRRFVAEGEVSYFYQQDETEKKDFMINSATGLYLQA
                     NFDEDSKLQNMQMKTDIKGKYVFKK"
     gene            complement(9274..9828)
                     /gene="lptC"
                     /locus_tag="PLG69_04670"
     CDS             complement(9274..9828)
                     /gene="lptC"
                     /locus_tag="PLG69_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPI42837.1"
                     /translation="MKKRFWLIWIVSVSAFILLTSCQQDSLQSATGVLPRDLPDEISY
                     NVKLTQLDNDRSEYYLEAEKIERFYDRRLLYAYKVTLTTYDKNNQISSVIKADTTIVD
                     DARNLIFANGNAILTSPNGTVATQKMVWDRSVDEITAPLMVTITRAGDVMRGQNLRTN
                     SQLSYVEMDAVSAEGIVKEKEIKW"
     gene            complement(9788..10348)
                     /locus_tag="PLG69_04675"
     CDS             complement(9788..10348)
                     /locus_tag="PLG69_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPI42838.1"
                     /translation="MYSKTTLVKPNKRVVPWNDLKLMVFDCDGVLTDGRFIYGSTNLE
                     LKHFHGHDGLGFKLLQKTDIMISVISGRSSEALERRCRDLNIPYLFQNITNKLKCLDN
                     LLKDLKLSYTNVAYMGDDWNDVPVMRKVAFSAVPADAQPDVAKIADWVSEYRGGQGAA
                     RDLINYVLMRKGIYEKTVLAYLDSIG"
     gene            complement(10370..11164)
                     /gene="kdsA"
                     /locus_tag="PLG69_04680"
     CDS             complement(10370..11164)
                     /gene="kdsA"
                     /locus_tag="PLG69_04680"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPI42839.1"
                     /translation="MNKLYTELASSQNFFLIAGPCVIEEESIMFTVAETLKELCARKG
                     IPFVFKSSYSKANRSSVNSFSGPGREEGLKILSKIKREFDVPVLTDVHECVEVASAAE
                     VCDILQIPAFLSRQTELIKTAAVTGRIINIKKGQFMAPEDMKLAAEKASSTGNKQILL
                     TERGTTFGYHNLVVDFRSFVIMHQFGYPVVYDVTHSLQRPSVGNYSGGNPEFALPMAK
                     AAIATGMVKGLFIETHPEPQQAKSDSASMLPLAKMEALLESCLQVL"
     gene            complement(11196..12362)
                     /gene="metK"
                     /locus_tag="PLG69_04685"
     CDS             complement(11196..12362)
                     /gene="metK"
                     /locus_tag="PLG69_04685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPI42840.1"
                     /translation="MTAALHAINNTDRNGYVFTSESVSEGHPDKICDQISDAILDAYL
                     KADPHSKVACETLATENRIVLSGEVSSRATIDIETIVRKTIKDIGYIHPDKGFDYNCE
                     INNYLHKQEAALEENTGAGDQGLMFGYACSQTKNLMPVPIELAHKLLFNLASARKNGT
                     IKHLLPDSKSQVSMRYLGRKAVDLKCLVISTHHDIVNETEFEELKEQIKQLVILPSIA
                     DIEDECKGYFDTKNCEIKINPLGPWKDGGPAFDTGLTGRKIIVDTYGGWAQHGGGAFS
                     GKDASKVDRSAAYMARHIAKSIVASGLAGECLIQFSFVIGEHKPVSLMVDTFGSGKES
                     DAEIERIIARNFDLTVEGIIDYLDLRKPIYFPTASYGHFGREGFSWERVKHISR"
BASE COUNT         3688 a         2609 c         2091 g         4168 t
ORIGIN      
        1 ataaaaattc cgctattcag ggcagttcct ataagttttt gcatttattc tccaaaaccg
       61 ttttcaatca tttccttaat ttctgccagc aaataatcct catccacacc attggcaata
      121 agttgcagtt tggaaccgca ttcggcagct aacatcataa cgcccataat gctttttcca
      181 tttaccctca ttccctcttt ctcaataaaa aattcactgc gatattttgt ggcagcttta
      241 accaaaagtg ctgagggcct tgcgtgcaaa cctaacttat tggttactgt cacttctacc
      301 tgtttcatca cctttgcctt caaaattttt ttgcattgtt ttcttacgga tttcatcgtt
      361 tattttgcgg ttaaagtcct cagctgcatc atatcccaca ctttttaaaa taacattcat
      421 tgcagctact tccacaataa ccgaaacatt ttttccggga gaaacaggca aatagatgat
      481 aggtattttc actcctaaat gttcagcata attattgacc aaaccaatgc gttcataatc
      541 catattttcc tgccagggca tcagctctat ctgcaggtct atagtttttt gtttgcgcgt
      601 atgagcaatg ccaaaaacgc gttccgcatt cacaaagccc acacctctaa tttccataaa
      661 atgtccaata tcccttcccg ccttgccaat caattgatcg tctatatatc gtaaagtgat
      721 taaatcgtct cccactaagc tatgtccacg gtgaacaagg tctaaagcac attcactttt
      781 tccgattccg cttttaccgg taatcaatat tcccaaaccg aaaacatcaa ccagcgtagc
      841 atgaatggtc ttttccagga caaaaatttc tcgtaaataa cgggaaagat tatcaattaa
      901 attaatagtg gaaagccggc tggaaagaag tgcaatattc aattcgttgg caagatattc
      961 aatcaccgga ggcaaagtaa gacctttggt aactataatg cagggaatat ccattttgaa
     1021 catctcccga atgcgggaaa ttaaaacctc ttccggtaaa ctttgtaaat agcgaacttc
     1081 cgtttctccc aaaatctgta ttctgtctgc ggcaaaaatt tccatatagc cagcgagagc
     1141 taagccggga cggttaatat gaggtgaacc gattttcttg ttcaaagttt ccggttcagt
     1201 tattaaagat agcgccaggt ctttcttttt ggcgtcgaaa aagtcattga cggtaatttc
     1261 tttcatctaa gttcctcgct tcatccggga tggcattttc ctttttacat tctgtagaat
     1321 cggaatttaa ctacccctca cgagtaacaa attccgtttt gccacagcta atgaagtcag
     1381 ttatttgatt cttcgcttaa ctttctcccg aaaaggaatt ctgcaaatct gggaaaactt
     1441 ttttgccgaa gtggaattcg gcagacattt tatcggtaga actttatggt tccagcagtt
     1501 tataaagctc gtggtcttta gttaccagaa catttatttt atcactttca atattcttaa
     1561 aaatcaggaa atcctctttg cttgcttcca gtttttccag agcttcagac aaactaaggg
     1621 attcggttac gactttttta gtttttattg tttttcggct gtgatttttc gcatccagct
     1681 gaaaatcggt agcgtaggaa tatgtctcaa attgttgttt taatgctctt ttctggtggt
     1741 ccgtaatgcg atcttttagc tttttaattt gaccttccat cttttccaca gcattatcaa
     1801 tagcaagata catatccatt tcttcagctt cactttgaag cgaaaatttg gcagcgtgaa
     1861 gggaaagttc acagatattg cggctgtttt ccaaagccaa aacggtatgt atagtgataa
     1921 tttggtcaaa atacttattc agcttggaac aagctgtttc tacataatca cggatagctt
     1981 tggtaagctc aaaatggcgg gctgtaatag tgatctgcat tgggggttcc tcctgttaga
     2041 ttttatgaat tgatttactt tgcaggtctt ccattgcttc cattattgct tctgaaaggg
     2101 tcggatgagc aaaaacaaag gaatcaatgt cttttgcctt atttttattg acgatcataa
     2161 tggttccctg cgcaataagt tctgcagcat tgggacccat aatatgcatt cctacaagtt
     2221 ctgatgtatc tttacgggca atggttttca caaaaccgtt aatattaccc attgccactg
     2281 cttttccatt tgctgcaaaa ggaaattttc ccactatcac atctccgaaa atttcttcag
     2341 catccttttg cgtgtagccc acggaagcaa tttcgggatg tgtaaaaatg cagcgcggaa
     2401 tattattata atttaacggt tcttgaggtg caaagtattt ttccggataa aggcgtgaag
     2461 caatatgttg tgctgccatc agaccttgtt tactagcagt atgagcaagt tgcatttttc
     2521 cggtaacatc gcctatagca tagattttat cgcagctggt ctgcataaag tcattaatta
     2581 caattgcacc tttttcctca tttatgttta ttcctttcca ggtaaggtta ttaaccggtt
     2641 ttctgccaac gcttaacaaa accttatctg ctgtcaaagt tgtattattg tttagtttca
     2701 attccaggcg cttatctttt ttaataatct cttgcactcc cgtatttgtt ataaccttca
     2761 ctccgttttt tttgaattgc atagcaagtc gtttggaaat ttcttcttct tcggtagcta
     2821 taaggtgcgg cagaaattct ataattgtaa cctgcacccc gaaagcagaa aaaatagagg
     2881 caaattcaca accgataact cctccgccta taatagcaag ctctttaggc aattcctgca
     2941 ttttcaaaat gccggtggag gaaaggatat ctttttcgtc aattttaatg ccggggagtt
     3001 cttttgtttc actgccggta gccaaaatca catattccga actgtaactt tctccttcta
     3061 aagtggttac gatgtaattt cccttttcct ttgtcacgga gacagcattt gctttaatca
     3121 caggaatatc gcgcttggaa aaaagaaatt caattccgct tgctaattgt tctacaacgg
     3181 catttttgcg ttcccaaacc ttgttatatg ccaaagttat ttccggttta ggtaagccga
     3241 aattatccgc attgagcatt tctgaatata attcggcact ttttaccaat gcttttgtag
     3301 gtatacaacc ccaatttaaa caaagtcctc cgagccgttc tttttccata agtaagctgg
     3361 aaatgccata ttgagaaagg cgaatggctg attcataacc gccaggacct cctccaataa
     3421 cgatcacttg gcaatgttgc atttattgtc tcctgagatg gctgttcaaa atgcccaatt
     3481 catcacggta tttagtaata attctccggg aaatatttaa gccctccgct ttaagaagtt
     3541 ctaccagttc ctggtcacta tagggttttt tcttatcttc catttcaatt aagcgaatta
     3601 aatgggtttt cactttttgg cgggaaacgc tttgataatc atcatcaaag ccagcggtgg
     3661 aagtgaaaaa atccttgaaa gcataaatcc cgtaaggtgt ttccgcatat ttatgtttaa
     3721 ctaccctgct gattgttgat tcgttaactc ctaaatctct tgcaattaca gcataggtaa
     3781 gaggtttcat ttgtccggta cccaaataaa agaaatcata ttggtgattg ataatagaaa
     3841 gggaaactcg ttccaaagtt cgcattcgca tataaatgga cttaattaag aatttggcag
     3901 aattgatgcg ttctcgcaca tattgcatcg tttctttgtc aaaatatccc cggttaatca
     3961 ttttacgata gcggggactg ataataatat tgggagtgat atgatcgttg ataatgattt
     4021 catatttgcc gtctatcagt ttcattgtaa catcgggaaa aacataaacg gcacttgtgg
     4081 aaagtatacg taagccaggt ttgggatcaa gcttggagat ttgttctttg gctaataaaa
     4141 tggtctcttc cgaaacaccg taggcagaag caattttttg ataacgacgg tgaataagat
     4201 tttcaaaatg ttcggaaacc aagcccacaa gaatgcggtt ttgtttttgt tcttccgtaa
     4261 gctgagccat caaacattct gttatattgc gggcagatat acctttaggg cttaaggaca
     4321 gaataatatt atgcagttct tcagctcgtt ccgccgtaat taaatacttt tttgccaaat
     4381 taacgatgtt gaaaccctta ggtaaaaatc cgtaactatc acaggaatca acaacatcgg
     4441 taataaaatc catttcgttt tccggcagtt ccaagggaaa aagttgttcc agaaattttt
     4501 ctttgccatt ttcttcatag cggataagcg cttcggtatg atccgtttca ctatcggaag
     4561 accagccctc atagctggaa tggtattcat tccaagtatc caaaatttct gtcagttctt
     4621 cagcttccgc caatgtttcc aaatggtttt cgctcatatc gctttcttca atcggagtgg
     4681 aactttcagg agtggattgc tcaaaattgc tttcatcaat atcatcttcg tctttttctt
     4741 cccgcaattc cagcaaagga ttatttacca attcctgttt aatatagctt tccagctcca
     4801 gaatgggtaa agcgagcatt tttaaggact ggagcatttt gggtttaagt gccagttcct
     4861 gtttttgttt taaggacaga tattgttcca ttgcgctcat aatcgttgct caaaagggct
     4921 ttccagaaat ctttcgccca aatataattc cttggctttt tcgtcattta tcagttccag
     4981 ggaagaacct gaaacgataa tttttccttc atagataata taagcccgat ttacaatttt
     5041 caaagtttct aaaacattat ggtcggtaac cattatgcct atgtctttat ttctcaattt
     5101 ggcaataata tcctgaatat ctgctaccgc aataggatca attccagcaa agggttcatc
     5161 cataaaaaga aaagtaggat tagttaccaa agcgcgagta atttccagtt ttcttctttc
     5221 gcctccggaa agagtatatg ccttctgttt tgccaaaggg gataaaccta attcttccag
     5281 ggcttctgct aaacgatttt tctgttcttt tcggctgatt tttagggtct gtaaaattgc
     5341 cagaatgttt tcttccacgg ttaatttggc aaaaatggaa ggtgcctgag ccaaatagcc
     5401 caaacctaaa cgcgctcttt tatacattgg cttgtgagaa atatccatat catcgtaaaa
     5461 aaccttgccg ttattgggct ttgccaaacc aataatcata taaaaagtgg tggttttccc
     5521 agctccatta ggacctaaaa tgccaactac ctctccctga ctcatttcca gagaaaggtc
     5581 atttactacg gctcttttgc catatatttt tacgagattt tccgctcgga ttttatgctt
     5641 ttccataaaa taataagata aagaaagctg tttttttgtc aagaaatact tgcaagtgga
     5701 ttgaattttg ctaataaaag acggatagca gggaagaatg aattaagtca ggagtcctca
     5761 ctgccaaaat aacttttaat ctatttacga atttgtttgg ctgtgagaac tcctgacagc
     5821 aaaaaaaccg gatttttaaa ttggcaacta tctgtataat atccttgttc atccgcgtta
     5881 atcttttaaa tacaagtcct gataaatctg tgaaatctcc atacttgatc attttacaac
     5941 caagtaacac tcaagtaaca actccgtaac gagacaacgg aagtgttacg gaagtgttac
     6001 ggagtcgtta cttaatcgtc tatataacaa gtagttattt aatcaaactg aattccgatt
     6061 attaatccag attaaagata atttttaacc tttcaattac ttctttcaat cctatccggc
     6121 ggatacctaa attctttata tttattgttg tatcattttc tatcaggaaa atgaaatctc
     6181 taagatcgtt ttttacggaa agaggtaatt caattatttc atttggctca agaacaggaa
     6241 ataaacgaaa tacatcattt ttatgcttat ttagcttttg ggaagttatg ttttctcctt
     6301 cagctatatt tttagtcata ttcaaatatg ccttggcttt aagaatgata atgtatgcag
     6361 gtctcagaca agataacccg tttatatcca ttaaaccatt cttgatcaaa gaataataat
     6421 tatcatcaag aattatagct gaaagcgaag atagttcatt atctatggga atgggagtga
     6481 ttctgccata acctttaaaa tctattgtct cagggattct ggaaaaaagc tctatctgct
     6541 caggatacat tgcattaacc ggattgttaa accggtaaca ctttttttct tctttatttt
     6601 gaaaaatact tctataattg cctttttcaa taaactccca gaacgccctt ccaaattccg
     6661 cactaagtgc ttcaacaata aggacgatat ctaaatctct tgttgctctg aaagttaaac
     6721 ctgattgaga taaaatttct gaacttgcag ctcctccaat tagaacatat tgctccttat
     6781 aatcaataaa atgttctcta aaagtagtta gccctcttac catttaattt tctccatcat
     6841 ttcctccagt gccatttgca ctctttcatc tgattcttcc ttaaagcata aaaacaaaga
     6901 taaaggatca acatagtcct ctgaagtaat tagtttggga ttataacgcc ataattgcaa
     6961 ttcattcgtc tgctcttcct tatagggtat ctccactaaa tcgggaaatt tttttgcaat
     7021 aatgcgagta gttgcataac tttcataatt ttctggagtt aacatactat actttgatag
     7081 agcagaaaac cctgcaaggc agaaaggaac ctgccgattt agccgaaacc aaattacttt
     7141 attaatggga ttaaaaaaat aaggttgggc tttttcccaa agttctttac cggtaaaaag
     7201 ccaatgaata ggtttttctt tcccacattc atccctcgtt atcaactcgt attcagtcaa
     7261 ttctcggaaa gccctggata catacataac attcaaataa tactttttag catacattga
     7321 aacagttgaa agctgatatt ctttttccag tagcgtccta attattacaa cttgagttaa
     7381 aggactgaaa cgatttctct tttttacctc ttggggacag gattcgttaa aaagaattcc
     7441 cagatggggt aaatatattt gattatcagg tataataaat gatattctct ctttaatgaa
     7501 agctctcctt aaagatggat taatagattt acttaaatag attactttta ttccaaacct
     7561 attttccaaa atatgagagt gtaattttac gactttagct gttaacatat cttttccttc
     7621 aatcaaaaga taatgcttcc catttagggt aagatcatag aatatatatt tttctgaaaa
     7681 gaagtgagga agtttgtatc caatattact gtgtcttatt tctaccttaa catccagcat
     7741 attttccaga tactcttttg tttttaaggc gatattcttc atttttgtct cctcattaac
     7801 attttttggt gctcattaac atagtaactg ttaatgagaa aaaagcaagt taatgatcta
     7861 atttttatat gtgttatggt ttctatttat tttcccattc attccgaaca gtaagtcata
     7921 agtcgtcact gcctatataa ctttgcacct caataaaaca gcagaaataa tttataggca
     7981 aatttagcaa ttgagttgcc aattccaact taagcaaatc ctgaaggcat aaattccttt
     8041 agaaaccttc gctttctttg gcaaagaaag aatttgccaa ccccttttcc tccaacggta
     8101 ttattggaaa taaaagcgat aatgacttct aaatttctat ttttccgaag aaatcttctt
     8161 gacctttagc tttcaagctc tccagacctc ttaacattct tcatttcttg aacacatatt
     8221 tgcccttgat gtccgttttc atctgcatat tttgcagttt gctatcctca tcaaaattcg
     8281 cctgcagata aagtcctgta gcgctattaa tcataaaatc ctttttttcg gtttcatcct
     8341 gctgatagaa atagctcact tccccttcgg caacaaacct gcgtagttgt tcatccagaa
     8401 aatccatagt cacgatgtct gcatccactg tatttttctt ttctttaaag cgttcttcgg
     8461 caaaatcaat atggcaggaa tcaattaatt cggctttaac taatttcctt tctttcataa
     8521 agagatgaaa ttcggtagct ttagcatcgg caaaatcaga aataaatttc ggttcaccag
     8581 taaaaattgc cttttcttct tccagaaaat agattaaaaa atcgctggtg gtatggaatt
     8641 cacctctatc cacctgaaca ttgaaagtag caataagttt gcgttccggt tcaaaatatt
     8701 ccattttttc agccgctaca gcaagtgtat cttctattcc tgaagtaata tagggttcat
     8761 ccaacagcaa tgcatatcct tttcctctat cccattgtgc atatccacat ttcacccggg
     8821 cattttcctg ataatcaaaa ccgtgcactc tactccaggt agtaaggata tcattttttt
     8881 tgtcgtaagt ggcaaaatta cttttaatcc aacggtagat gccttcttta gttctttcgg
     8941 tagcagttat tctaccaccc aaattcatca aatccggaat acgataataa gccaaagtat
     9001 cagcttccag tgttaaacta tcatttttta caattacatt tcccgaaagt cgggcaattt
     9061 tttgtttatc taaaatcaga gcattccgac aaagaaattc cacctctcca taaaagaaat
     9121 gaacattgcc gttaagttcc aatgcctgtt cattttccat tttagtcata aacagtttat
     9181 cggaatgaac cagacggaat tcctcttccc cttttttttt gccaaataaa ggcagtaaaa
     9241 gaagaaacag cagaaagaaa acaaaaccct ttttcaccat ttaatttcct tttctttcac
     9301 aatgccctca gccgaaactg catccatttc tacataactt aactgactat tggttcgtaa
     9361 attttgccct cgcattacat ctcctgcacg agtaatcgtt accattaaag gagcagtaat
     9421 ttcatctaca gacctgtccc aaaccatttt ttgagtagcc acagttccat ttggagaagt
     9481 aagaatagca tttccattgg caaaaattaa atttctggca tcatcaacaa tagttgtatc
     9541 tgctttaatc acagaactta tctggttatt tttgtcataa gtggtaaggg ttactttata
     9601 agcatacagc aaacgcctat cgtaaaatct ttctattttt tccgcttcca gataatattc
     9661 actacggtca ttatccagtt gtgtcagttt aacattgtaa ctgatttcat cgggtaaatc
     9721 acgcggcaaa actcccgttg ccgattgcaa gctatcttgt tgacaggaag ttagcaaaat
     9781 aaaagcgcta accgatacta tccagataag ccagaaccgt tttttcatag atgccttttc
     9841 gcataagcac atagtttatc aggtctcttg cagcaccttg tccacctcta tattctgaaa
     9901 cccaatcagc tattttggca acatcgggtt gagcatcagc cggaactgca gaaaaagcta
     9961 ctttacgcat aaccggaaca tcattccagt cgtctcccat ataagcaaca ttggtataag
    10021 ataatttcaa gtcctttagc aagttatcca ggcatttcag cttattggtt atattttgaa
    10081 acagataagg aatattcaaa tcgcgacagc gtctttccaa tgcttcagaa ctgcgtccgc
    10141 tgataacact gatcataata tccgtttttt gtagcagttt gaaacccaga ccgtcatgcc
    10201 cgtgaaaatg tttcaattcc agattggttg aaccataaat aaaacgaccg tcagtaagca
    10261 ccccgtcaca atcaaagacc attagtttga gatcattcca gggaactacc cgtttattgg
    10321 gtttaacaag tgttgtttta ctgtacattt ctattttcct tatagttatt tataaaactt
    10381 gcagacagct ctccaaaagg gcttccattt ttgccaaagg aagcatactg gcagaatcac
    10441 tttttgcctg ctgcggttca ggatgggttt ctataaaaag accttttacc attcctgtag
    10501 caattgctgc tttagccatt ggtaaagcaa attcgggatt gccaccggaa taatttccca
    10561 cagaaggtct ttgcaatgaa tgagttacat cataaacaac gggatagcca aattggtgca
    10621 taataacaaa actgcgaaaa tccacaacca gattatgata gccaaaagta gttccgcgtt
    10681 ctgttagcag aatttgttta ttacctgtgc tacttgcttt ttcggcagct aatttcatat
    10741 cctcgggagc cataaactgc ccttttttta tgttaattat tctacccgta acagcagctg
    10801 tcttgattaa ttctgtctgc ctggaaagaa aagcgggaat ttgtaaaatg tcacaaacct
    10861 ctgcagcaga agcaacttca acacattcat gcacatcggt tagaaccgga acatcaaatt
    10921 cccgttttat tttactcaat attttaagcc cttcttctct tcccggaccg ctgaaggaat
    10981 ttacagaact gcgattggct ttgctgtaag acgatttaaa cacaaaagga atgccttttc
    11041 gggcacagag ttctttcaag gtctctgcca ccgtgaacat tatactttct tcttcaatca
    11101 cacagggtcc tgcaatcagg aagaaatttt ggctggaggc aagttctgta tagagtttat
    11161 tcattaattt cagaacagca atgctgtttt ctccttcatc tggaaatatg tttaactctt
    11221 tcccaggaaa aaccttctct gccgaaatga ccataagaag cagtaggaaa gtatatgggt
    11281 ttacgaaggt ctaaataatc tatgatgcct tccactgtca agtcaaagtt tctggcaata
    11341 attctttcta tttccgcatc gctttctttt ccactgccaa aagtatctac cattaaagaa
    11401 acgggtttat gttcaccaat cacaaagctg aattgaatta aacattcacc ggctaatcca
    11461 ctggcaacaa tacttttggc aatatgcctt gccatataag ctgcgctccg atcaacttta
    11521 gaagcatctt ttccggaaaa agcgcctcct ccatgttgag cccaaccacc ataagtatcc
    11581 acaataattt tccttccggt taatcctgta tcaaaagcag gacctccatc cttccagggt
    11641 cctaaaggat tgattttgat ttcacaattc ttggtgtcaa aataaccctt gcattcatct
    11701 tcaatatcgg ctatagaagg aaggataacc aactgcttga tttgttcctt cagttcctca
    11761 aattcggttt cattcacaat atcgtggtga gtagaaataa ccagacattt caaatccact
    11821 gctttacgcc ccaaataacg catagaaacc tgacttttgg aatcgggcaa caagtgtttt
    11881 atagttccgt tttttctcgc cgaggcaaga ttaaaaagca gtttatgtgc cagttcaata
    11941 ggaacgggca tcagattttt agtttgagaa caagcataac caaacatcaa accttgatct
    12001 cctgctccag tattttcttc taaagctgct tcctgtttat gtaaatagtt atttatctcg
    12061 cagttataat caaaaccctt gtccggatga atataaccaa tatccttaat tgttttacgc
    12121 acaattgttt ctatatctat agtcgcccgg gaagaaactt cccccgaaag aactatcctg
    12181 ttttccgtag ctaatgtttc acaagcaact ttggaatgag gatcagcttt taaataggca
    12241 tccaaaatag catccgatat ctgatcgcag attttatccg gatgaccttc cgaaacggat
    12301 tccgaagtga aaacatagcc atttctgtct gtgttgttaa ttgcatgtaa tgctgctgtc
    12361 atctatatct cctaattctt atttctttct ttctttgctg cccttaaaaa aagccggctg
    12421 ctaaagccgg caaaaatttt tataccgcac tttagcacat tttgactgcg gagcaaaggg
    12481 caaatcaccg ctcttctaaa aatggtacat cttcggggat tcgaaccccg gacccactga
    12541 ttaagagtca gttgct
//
LOCUS       DAOPFL010000047        12354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_6089_length_12354_cov_108.578177, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000047 DAOPFL010000000
VERSION     DAOPFL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12354
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6089_length_12354_cov_108.578177"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2369
                     /locus_tag="PLG69_04690"
     CDS             <1..>2369
                     /locus_tag="PLG69_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPI42841.1"
                     /translation="GFQLIEKKEIKEIKVTAYRYQHLQSGAELMHYECDDDNKVFMIG
                     FKTVPEDNTGCPHIMEHSVLNGSKNFPAKSTFMELVKGSMNTFVNALTFTDMTCYPVA
                     STNNKDFINLMRVYLDAVFFPNIYNEPNILHQEGWHLELTSEDAPLKYKGVVYNEMKG
                     ALSSPENSINRKSRQAQFPDTPYGFESGGDPEAIPELTYEKFIAFHQKYYHPANSKIT
                     LYGDMDIEETLKIIDEEYLSQFEDNNEKVQIPLQKPFGKTKVLKMEIPVEEGKDIEDQ
                     YYLALNWTYGQITDKYLSQTLKALAEMLMDTPASPLKKAIQDSGYAKDSTIVVEDDIL
                     QPTVFLLCKQVKKENIEPLTKLIKQELKRIVKEGLDKKLIEAVINKMEFNLREAQYRF
                     YPRGLIYALNSQGLWMHNGNPVDKLAFEPMLKELRKGLKEPYFEELIEKAILNNKHSS
                     QITFVPVPGLIQKMEQETAEKLAALKKKMTKKEIAKLIEFNKKLIQWQQEPETKENLE
                     KIPMLSLSDLNPEAKHYPTEEDIWNDIKLLKHPANTNGIVYFKSYFDLAHAEEEDLPW
                     IKLYTQLVEWMNSENYSYAKRSTEIDSNTGGIYLDIALYNSYQTPDDILPKILLRGKA
                     VKNKFGKLMELASDFALKPLFDEPERLKKLLAELKAKSEAMLSFRGHTIAIQRMLKPL
                     SQIYHWIDITNGLGYYHFLCDLVSNMDSAIDEIMDELNWIKKTFFTTHNMIISITADA
                     DIIPSAIEKLGTFADNVSTEAFAPVESHFAVRQFNEGIYAPVQVQFCAKG"
     assembly_gap    2370..2469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2470..3053
                     /locus_tag="PLG69_04695"
     CDS             <2470..3053
                     /locus_tag="PLG69_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HPI42842.1"
                     /translation="QFNEGIYAPVQVQFCAKGGNFFRKGFSYSGKLRVLNNILRSSYL
                     YQELRVKGGAYGNMSDFTLGGYLYFVSYRDPNLRETLEVYNTIPEFLRNFDCDKREFD
                     KYIIGEISSLDFPLTPEGMGDKADEDYITGFTFEDRQQIRDEVLSAKIEDMRNYAELI
                     EAVMSKNHYAVFGSETKVKEAADLFDAITPALR"
     gene            3077..4141
                     /gene="rsgA"
                     /locus_tag="PLG69_04700"
     CDS             3077..4141
                     /gene="rsgA"
                     /locus_tag="PLG69_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPI42843.1"
                     /translation="MKQKDKKKTKRWTSKIKNVHLPDIEILDEIKEKAEFKASRISTK
                     ANQSYKEGLKLVQGRIMEIKSNYRYIVEINGELVTAKMSGRLKQFLYQSHTIAAVGDF
                     VAVDVANNSDRRIEKILPRRSALIRYGTGSFQKEIILAANIDQVIITSSWRKPRFKAG
                     LIDRYLCIVAIQKLNPIIVINKIDLLEDEQELKEQTAYYEAMGYPLICTSAINGTGMQ
                     ELKELLKNKDSVFTGHSGTGKTSLINYLEPSLNLPTAEVSDRNEKGKHTTSQSILIPW
                     SFGGHLLDTPGIKTITLHREDKEHIPKVFPGFERLYSKCKFSDCRHINEDGCAVLEAM
                     EEGIIPYERYDSYQYLYASL"
     gene            4141..4995
                     /locus_tag="PLG69_04705"
     CDS             4141..4995
                     /locus_tag="PLG69_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPI42844.1"
                     /translation="MQNFAVYINPEFSDKKSIYNLLEKLRNDSDINFFSIESIPDLPK
                     ELFKPLPKPANSKHIDCILVFGGDGTILKAKDLALLTGAPILGINLGYLGFLSESVLP
                     EIASSIENLKQGKYRLLHRMLIECHLKREGKIIYEALALNDAVIHKAESPGLIHIRIK
                     ASGRYVFDTRCDGVIAATPTGSTAYSLAAGGPILAPEMKAIVLAPLNPHILAIRPMVF
                     PATERLAMKVYGLSQPAMLQIDGQNSQTIQEGDEVFVTASERSVSFIKLSNRTFYQIL
                     RRKLNLGK"
     gene            4995..6698
                     /gene="recN"
                     /locus_tag="PLG69_04710"
     CDS             4995..6698
                     /gene="recN"
                     /locus_tag="PLG69_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPI42845.1"
                     /translation="MLSELYIKNYLLVPEMRLSLDKGLTVISGETGAGKSIVAGSISL
                     IFGDNLAGIEAFDKDQPIYLEVSYNLEEAEELNNFLAQNGIETEEELILAREISPNGK
                     SGYYVNGRKVGVSLLKELKSYLIDFHHQRDQQRLLSPSYQLDLLDAYADLTSIREEFA
                     NLYRELKAGLKKLDELKNEAERQKEINELYRFQYEELENAKLRINEDVELQNEYELLS
                     HTLEITELYNQITAELLEQENSVFDIISGYLSKLKHYEHLNPEIGYAVNNMQQALENL
                     QEASLNLSRSKDNISFEPQHLQDIQARLDLINSLVHKHKVRSIEELLNLFAERSAQIA
                     AFADNEKSIAELEKHLEKNFAELRKKGEELSRKRQKSAIELSKELQENIRVLSIPEGI
                     LEIIIDKKAEDNFVISDYLSAVSESGQDSVEFLFSANPGFELKPLSAVVSGGELSRIL
                     LAIKKVLADRLEPKLIILDEIESGIGGKTAAIVADFIAKLAERQQVLCITHLAQIAGR
                     AEKHLVIEKFTQGEKSFVNIRKVEAEQRLQEIARMLSGTLTAKALEHAAEILKDINIR
                     G"
     gene            6700..7620
                     /locus_tag="PLG69_04715"
     CDS             6700..7620
                     /locus_tag="PLG69_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42846.1"
                     /translation="MKKREKRRVRGIAIFIVFFAIAASIVQWQNKAGKHETYNFKPAQ
                     NEYRDLKFLNKAQISFSSPNVAKAKTEIEKIISEHSTKQIRKQSEGSFGAYIFSVPQK
                     DLPIIVEKLRSFGSVNSQTEQIDTALVNIDYESELVRLQSYEREQAELNNVRFPTDTQ
                     NRRKEELHSLIQSTRSNLDKLKESNNVLLFITLVPQQKTNTIEVTIKSLALNFFSWLI
                     IFAVGAVLAYYGTRLLMYFLAALGVKGIGAGGLGGSYRYGGYGGYYNRYYNRYGYGHG
                     RRKVKRIYKDKRTTPVSEDEDTEQSEEGKQ"
     gene            7621..8064
                     /locus_tag="PLG69_04720"
     CDS             7621..8064
                     /locus_tag="PLG69_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPI42847.1"
                     /translation="MKLLPWQIWMILGIGFIIIEIFDPAFFFISLGIGAIITSLLSLI
                     PVLGNSVPLQILIFAVLSFISFLFMRKLGKKLLSKPGGNTNVYALKGQNGFVTKEIPA
                     EGKGYVKIGGEEWPAIEVENKAVELGAKVVVEGIEGNKVIVRKIP"
     gene            complement(8229..8873)
                     /gene="nth"
                     /locus_tag="PLG69_04725"
     CDS             complement(8229..8873)
                     /gene="nth"
                     /locus_tag="PLG69_04725"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPI42848.1"
                     /translation="MQNRDIDTVMERLKKHFYSVKTPVVDLIQIKTEDPFKVLVTTIL
                     SARTKDETTAKVAEKLFPKVQKIEDLEKIPLAELDTLITPVGFHRVKAKHLKELPKVL
                     KEKFNGKIPEEIDDLLELPGVGRKTANLVRAVAFQKPAICVDVHVHRICNRWGYIQTK
                     TPLETEMTLRQKLPEKYWLNFNSYLVAFGQNLCTPRKPKCEICPVAEFCKRVGV"
     gene            8976..9647
                     /locus_tag="PLG69_04730"
     CDS             8976..9647
                     /locus_tag="PLG69_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPI42849.1"
                     /translation="MCGRFAQVIKYQDLKKMTEELKIKESSEQLELNYNVAPTNIVAA
                     VVAKDDWRYLGFFRWGLIPSWSKKIPEQALINVRSESILEKPSFKTSFLRRRCLIPAN
                     GFYEWRKTDKQPFFIKAKGDNLLYLAGIYDAWYGPDGSYIPSLGIITTSANDFIQPLH
                     ERMPLLLNPSLYDTWLNPAAQNPQELQLLLTVPSEIELEMYPVSRRVNKPENNDADCL
                     KPIAL"
     gene            9681..10763
                     /locus_tag="PLG69_04735"
     CDS             9681..10763
                     /locus_tag="PLG69_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPI42850.1"
                     /translation="MDNLKKQILIVDDDLAILDCIRMSLQKDFPCRITTCSDSREALE
                     LLESNIFNVIISDIGMPNLSGFQLLEYINKLNPNIPVILITGEVDTEKIRSAVQLGAF
                     DILHKPFELSDLHISVKQALQKNSLLLQNEMYRLNLEELVEKRTKELYIAQCQLEKNY
                     LNTIHAMVNAMEANDVYIRGHSERVTVLSIMLGKIIGLDAEDLKLLRIGAILHDLGKI
                     GIYDTLLNKKGSLTSSEYEMVKQHPVIGAKIIRPIGLPTPVYEIILQHHEWFNGKGYP
                     YGLTGNQISPLARIVTIADAYDAMTSKRAYRENLDAKSARQEVINNAGIQFDPEYGKV
                     FFDNYSHIVEPVSDSPAIQNLLFDML"
     gene            10794..11450
                     /locus_tag="PLG69_04740"
     CDS             10794..11450
                     /locus_tag="PLG69_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPI42851.1"
                     /translation="MVQIISAAEAAAMIKPGNRLAIGGFLAVGAPEKIIDAMVENGNK
                     DLHIIVIASDWEDRGVGKLVVNKMVKSAQVSHMGTNKTIQAQMNAGEIDIELVPQGTL
                     MERVRAFGAGLGGILTPTGLGTIVAEGKQIITVDGKDYLLETAIPSDFALIKAHKADK
                     LGNLTYRKTARNSNPIMAMAGKITIAEVDEIVEIGELDPEEVITPGVFVNYLVLAKEN
                     "
     gene            11452..12105
                     /locus_tag="PLG69_04745"
     CDS             11452..12105
                     /locus_tag="PLG69_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HPI42852.1"
                     /translation="MALDKRAMIGARIAKEFKDGDYVNLGIGLPTEAANHIPEGISVI
                     FQSENGLLGAGPKPPEGQEDKDMINAGGGYITCIPGASFFDSATSFAIIRGGHIDATV
                     LGALQVDQEGNLANWLIPGKMVPGMGGAMDLVTGAKKVIVAMEHCDKYGNSKILKKCT
                     LPLTAKGKVNLIITDMAVIEVCPEGLYLREIAEGLTIDDVVQATEAELIIPERVNTF"
BASE COUNT         3980 a         2272 c         2516 g         3486 t
ORIGIN      
        1 ggcttccagc tgattgaaaa gaaagaaatt aaagagataa aggtaactgc ctatcgctat
       61 cagcatttgc aaagcggtgc agaacttatg cactatgaat gtgatgatga taacaaggtt
      121 tttatgatcg ggtttaaaac agttccagaa gataataccg gttgccctca tattatggaa
      181 cactcagtcc tgaatgggag taaaaacttt cctgctaaaa gcacttttat ggaactggtt
      241 aaaggaagta tgaacacttt tgtgaatgct ttgaccttta ccgatatgac ctgctatcct
      301 gtagccagca ctaataataa ggattttata aacctgatgc gggtttacct ggatgcggtg
      361 tttttcccca atatttataa tgaacccaat attttacacc aggagggatg gcatttggaa
      421 ttgacctccg aagatgctcc tttaaaatat aagggtgttg tttataacga aatgaagggt
      481 gctttatcct ctcccgaaaa cagtatcaat agaaaatccc gtcaagcaca gtttcctgat
      541 acgccctacg gttttgaaag cggaggtgat cctgaagcca taccggaact aacttacgaa
      601 aagtttattg cttttcatca aaaatactat catcctgcca acagcaaaat aactctttat
      661 ggcgatatgg atattgagga aaccctgaaa ataatagatg aggaatacct ttcccaattt
      721 gaagataaca acgaaaaagt gcaaatccct ctccaaaaac ccttcggcaa aaccaaagtg
      781 ctgaaaatgg aaattcctgt ggaagaaggc aaagatattg aggatcaata ttatcttgcc
      841 ctcaattgga cttacggaca gattaccgat aaatatctgt cgcagacatt aaaagcatta
      901 gcggaaatgc tgatggatac tcctgcttca cccttaaaaa aggcaattca ggattctggc
      961 tatgctaaag acagcacaat cgttgttgaa gatgatattc tgcagccaac cgtatttctg
     1021 ctctgtaaac aagtgaaaaa ggaaaatatt gaaccattga ccaaactgat caaacaggaa
     1081 ctgaaaagga tagtgaaaga aggtctggat aaaaaactga ttgaagcggt tatcaataaa
     1141 atggaattta acttgcggga agctcaatat agattttatc ccaggggctt gatttatgcc
     1201 cttaattcac aaggactttg gatgcataac ggcaatccgg tagataaact tgcctttgag
     1261 ccaatgctga aagaactacg caagggactg aaagaaccct attttgaaga acttatagaa
     1321 aaagcaatcc tgaataataa acactccagc caaattactt ttgttcccgt tccaggtttg
     1381 attcagaaaa tggaacagga aaccgcggaa aaattagctg ctctgaaaaa gaaaatgacc
     1441 aaaaaagaaa ttgccaaact aattgaattc aacaaaaaac tgattcaatg gcagcaggaa
     1501 cccgaaacca aggaaaatct ggaaaaaata cctatgcttt ccttaagtga cctcaatcct
     1561 gaggcaaaac actatcctac cgaagaagac atctggaacg atattaaact gctgaaacat
     1621 cccgcaaaca ccaacggtat tgtctatttc aaatcctact ttgaccttgc ccatgcagaa
     1681 gaagaagacc tgccctggat taagctttac actcaactgg tggaatggat gaacagtgaa
     1741 aattacagct atgcaaaacg ctctacggaa attgatagta ataccggtgg aatttatttg
     1801 gatatagctc tttataacag ttaccaaacc ccggatgata ttttacccaa gatcctttta
     1861 cgcgggaaag cagttaaaaa caaattcggt aaactaatgg agcttgcttc cgattttgcc
     1921 ctaaagccgc tttttgatga accggaacgc ttaaaaaaac tactggcaga attaaaagcc
     1981 aaaagtgaag caatgttatc cttcagagga cacacaattg ccattcaaag aatgctaaaa
     2041 cccctttctc aaatatatca ttggatagat ataactaatg gactgggtta ctatcatttc
     2101 ctttgtgacc tggtgagcaa tatggattct gccattgatg aaataatgga tgaactgaac
     2161 tggattaaaa agaccttctt tactacccat aatatgatta tcagcattac ggcagatgca
     2221 gatataatac cttctgccat agaaaaactg ggaacttttg ccgataatgt ttctaccgag
     2281 gcatttgcac ccgtagaaag tcattttgct gtgcggcaat tcaacgaagg tatttatgct
     2341 ccggtgcaag ttcaattctg tgccaaaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnng gcaattcaac gaaggtattt atgctccggt gcaagttcaa ttctgtgcca
     2521 aaggcggtaa ctttttccgt aagggctttt cctacagcgg taaacttaga gttctaaata
     2581 acattctacg gagtagttat ttgtatcagg aacttcgcgt taaaggtggc gcctacggaa
     2641 atatgagtga ttttaccctt ggcggttatt tatactttgt ttcctatcgc gatcctaact
     2701 taagagaaac cctggaggtc tataacacca ttcccgagtt cttaaggaac tttgattgtg
     2761 ataagcggga atttgataaa tatatcatcg gtgaaatttc gtctttggat tttcctttaa
     2821 ctcctgaagg aatgggagat aaagcggatg aggattatat caccggtttc acttttgaag
     2881 atagacagca aattagggat gaagtgcttt ccgctaaaat agaagatatg agaaactatg
     2941 ccgaactgat tgaagcggtt atgagtaaaa atcactatgc cgttttcggt tcggaaacca
     3001 aagtgaaaga agccgcagac ctctttgatg ctataactcc cgcacttaga tagaccccct
     3061 tttgccctgg agggaaatga aacaaaaaga taaaaagaaa acaaaacgct ggacaagtaa
     3121 aataaaaaat gtccatctgc cggatattga aatcctggat gagattaaag aaaaggcaga
     3181 atttaaggca tcacgcatca gcacaaaagc aaaccagtcc tataaggaag ggcttaaatt
     3241 ggtgcagggc agaattatgg aaattaaaag caactaccgc tatattgtag aaattaacgg
     3301 tgaactggta acagctaaaa tgagcggacg actgaaacag ttcttgtatc aaagccatac
     3361 aattgctgcc gtaggagatt ttgttgctgt agatgttgcc aataattcag atagacgcat
     3421 tgaaaaaatt ctgccccgtc gttccgctct aattcgttac ggaacaggga gttttcaaaa
     3481 agagattatt ctggctgcca atatagatca ggtaattatt acctcttcct ggagaaaacc
     3541 aagatttaag gcaggactta tagaccgcta cctttgtata gttgccatcc agaaattgaa
     3601 ccccatcatt gttatcaaca aaatagacct gctggaagat gaacaagaac taaaagaaca
     3661 aactgcttat tatgaagcaa tgggttatcc tcttatttgt acttctgcaa ttaacggaac
     3721 cggtatgcag gaattgaagg aattgttgaa aaataaagat tcagtgttta cagggcattc
     3781 cggaaccgga aagacctcgc taataaacta tttggaaccc tccttaaacc ttcctacagc
     3841 agaagttagc gaccgcaatg aaaaaggaaa acatactact tcacaatcaa tattaattcc
     3901 ctggagtttt ggaggtcatc ttttagatac cccaggaatt aaaaccatca ctttgcatag
     3961 agaagataaa gaacatattc ctaaggtctt ccccggtttt gaacgcttgt atagtaaatg
     4021 caaatttagc gattgccgtc atattaatga agatggctgt gccgttttgg aagcgatgga
     4081 agaaggaatt attccttatg aacgctacga tagctatcaa tatctttatg cgagtttata
     4141 atgcagaatt ttgcggttta catcaatcct gagttcagtg acaaaaaaag catctataac
     4201 ttgctggaaa aattaaggaa cgattccgac atcaatttct tcagcataga atccattccc
     4261 gatttaccta aagagctatt taagccctta cctaaaccgg caaacagtaa acacattgac
     4321 tgcatcctgg tttttggcgg tgatggaact atcttaaaag ccaaggattt ggctctgtta
     4381 accggagctc ccattttagg aattaacctt ggctatttgg gtttcctttc cgaaagtgtt
     4441 cttcctgaaa ttgcttcatc cattgaaaac ctgaaacagg gaaaataccg tttactgcat
     4501 agaatgttaa tagaatgcca tctgaaaagg gagggaaaaa ttatctatga agcattggca
     4561 ttaaatgatg ctgttattca taaagcggaa agtccgggct taattcatat tcggattaaa
     4621 gccagtggac gctatgtatt tgatacccgt tgtgatggag ttatagcagc tactcctacc
     4681 ggttcaactg cctattcact tgcagcagga ggtccgattt tagctccaga aatgaaggca
     4741 attgttcttg ctccgctcaa tcctcatatt ttagctatca gacctatggt ttttccagca
     4801 acggaacgct tagcgatgaa ggtttatggc ttaagtcaac cagcgatgtt acaaattgac
     4861 ggtcaaaatt cacagaccat tcaagaaggt gatgaggttt ttgtaactgc ttccgagcgt
     4921 agcgtttcct tcatcaagct ttccaatcgg actttctatc aaatcctgcg caggaaactc
     4981 aatttgggca agtaatgtta tccgagcttt acataaagaa ttatcttctg gttccagaaa
     5041 tgcgcttatc tttggataag ggcttaaccg ttatcagtgg agaaacggga gccggtaaat
     5101 ctattgtagc aggttcaatt tctcttattt tcggagataa cttagccggc attgaagcat
     5161 ttgacaaaga tcagccaatc tatctggaag taagctataa ccttgaagaa gcggaagaat
     5221 tgaataactt ccttgctcaa aacggaatag aaacagagga agagttaatc ttggcacggg
     5281 aaataagtcc taacggcaaa tccggctatt atgtaaatgg acgcaaagta ggagtaagct
     5341 tactgaaaga attgaaaagt tatctcattg attttcacca tcaaagggat caacagcgtt
     5401 tgctttctcc ttcctatcaa cttgatttat tggatgctta tgctgatttg acaagcatta
     5461 gagaggaatt tgccaattta tacagagaac tgaaagcagg actgaaaaaa ctggatgaac
     5521 tgaaaaacga agcagaaaga caaaaagaaa taaatgaact ctatcgcttt caatatgaag
     5581 aactggaaaa tgccaaacta cgcataaatg aagatgtaga actgcaaaat gaatatgaac
     5641 tgctttccca caccttggaa ataactgaac tatataacca aattactgca gaacttttag
     5701 agcaggaaaa cagcgtcttt gatataatca gcggttactt gagcaaactg aaacattatg
     5761 aacaccttaa tcccgaaatc ggatatgccg taaacaatat gcaacaggca ttggaaaacc
     5821 tgcaagaagc atccctcaat ctttcccgct ccaaagataa tatttccttt gaacctcaac
     5881 atttgcagga tattcaagct cgcctggatt tgatcaattc tctggtgcat aaacataagg
     5941 ttcgcagcat tgaagaactg ctcaatcttt ttgccgaaag gtctgctcaa attgccgctt
     6001 ttgccgataa cgaaaagtca attgcagaat tggaaaaaca cttggaaaag aactttgccg
     6061 aattaaggaa aaaaggagag gaactttctc gcaaaagaca aaaatcagct atagaactta
     6121 gtaaggaatt gcaggagaat atccgtgtct taagtattcc cgagggaata ttagaaatta
     6181 taattgacaa aaaagctgag gataattttg taatttctga ttatttatct gccgtttcgg
     6241 aaagcggtca ggatagtgtg gaatttcttt tttccgctaa tccgggcttt gaattaaagc
     6301 ccttatctgc agttgtcagc ggaggtgaat tatccagaat tttgctggct attaaaaaag
     6361 tactagcaga ccgcttggaa cccaaactga ttattttgga tgaaatagaa tcaggaattg
     6421 ggggaaaaac agctgctata gtagctgatt ttattgccaa actggctgaa aggcaacagg
     6481 tgctttgtat tacacacctg gctcagattg caggaagagc cgaaaaacat ttggtaatag
     6541 aaaaatttac tcaaggtgag aaaagttttg tgaatatacg caaagtggaa gctgaacaac
     6601 gcttgcagga aatagccaga atgctttccg gaaccttaac tgccaaagct ctggaacatg
     6661 ctgcagaaat attaaaagac ataaacataa gaggttaatg tgaagaaaag agaaaaacgc
     6721 agagttagag gaatagctat ctttatagta ttctttgcga tagctgcctc aattgtccag
     6781 tggcaaaata aagccggaaa acacgagacc tacaatttta agccggctca aaatgaatat
     6841 cgggacctca aatttctcaa taaagctcaa atatcatttt cttctccgaa tgtagccaaa
     6901 gctaaaaccg aaattgaaaa aatcatcagc gaacattcta cgaaacagat tagaaaacaa
     6961 agtgagggct cttttggtgc ttatattttt agtgttcccc aaaaagacct gcctattatt
     7021 gtagaaaaat tacgcagttt tggttcagtg aactctcaaa cagaacaaat tgatactgct
     7081 ttggtgaata ttgattatga aagtgaactt gtccgcttac agagttatga aagagaacag
     7141 gcagagctaa ataatgtccg ttttcctacc gatactcaaa acaggcgcaa agaagaatta
     7201 cattccctga tccagagcac gcgttccaat ttggataaac tgaaggaatc aaataatgtt
     7261 cttcttttta ttactttggt tccacaacaa aaaaccaata ccatagaagt aaccattaaa
     7321 tctttagctc tcaatttctt cagttggctt attatttttg ctgtgggagc agttctggct
     7381 tattatggaa ctcgtttgct aatgtatttt cttgccgctt taggagtaaa aggcatcgga
     7441 gctggtggtt taggtggttc ttatcgttat ggcggatatg gaggttatta caatcgttat
     7501 tataaccgct atggatatgg tcacggaaga cgtaaagtta aacgcatata taaagataaa
     7561 agaacaaccc ctgtatccga agatgaagac actgaacaat ccgaagaagg aaaacaataa
     7621 atgaaacttt taccttggca gatatggatg atattaggaa tcgggtttat cattattgaa
     7681 atctttgatc ctgcattttt ctttatttcc cttgggatag gagctatcat aacttctctg
     7741 ctttctttga tacctgtttt aggtaatagt gttcctctgc agattcttat ttttgcggtt
     7801 ctcagtttta tttcttttct ttttatgcgt aagctgggca aaaagctttt atccaaacca
     7861 ggtggaaata ccaatgttta tgccttaaag gggcagaatg gctttgtcac caaagagatt
     7921 cctgcagaag gaaagggtta tgtaaaaatt ggaggagaag aatggcctgc gattgaggta
     7981 gaaaataagg cagtggaact tggagccaag gtcgttgttg aaggtattga aggtaataag
     8041 gttatcgtta gaaaaatccc ataaccgatt tcttttcctt cctgtttttt attgtcattc
     8101 ctgcttccat tgtaattcct gggcaagcag gaatccatac aatatcatca tccccaaaat
     8161 ccctataatc ctgttcatcc ctaacctgtt tttatatgtg taatcggtga aatctgtaag
     8221 aggatttttt atactccaac ccgcttacaa aattctgcta cagggcatat ttcgcatttg
     8281 ggtttgcgcg gagtgcacag attttgtcca aaagcaacca aataggaatt gaaattcaac
     8341 cagtattttt ccggcaactt ttggcgcagg gtcatttccg tttccaaggg tgtttttgtc
     8401 tgaatatagc cccaacgatt acaaattctg tgcacatgaa catccacaca gatagcaggt
     8461 ttttgaaaag ctacagcacg aaccaggttt gccgtttttc ttccaactcc gggaagttcc
     8521 aataaatcgt ctatttcttc gggtattttt ccgttaaact tttctttcag cactttaggc
     8581 aattccttca aatgtttagc ttttactcta tgaaaaccaa caggagtgat aagtgtgtcc
     8641 aattcagcca agggtatctt ttccaggtct tctatctttt ggacttttgg gaataatttt
     8701 tctgctactt tcgctgtagt ttcatctttg gttctggcgc taagaatggt agttacaagc
     8761 actttgaaag ggtcttcggt tttaatttga ataaggtcaa ctacgggtgt tttcacgctg
     8821 taaaagtgtt tttttaggcg ttccatcacg gtgtctatat ccctattttg cattctgtct
     8881 cctgtaataa aaaagcaata gctgtaagct gttaaaaaaa attattgcct aaaaaatggc
     8941 tttcataaat cttgtcaatc aataaggaga aaaggatgtg tggacgtttt gcccaggtta
     9001 ttaaatatca agacctgaaa aagatgacag aggaactaaa aattaaggag agctcagagc
     9061 agctggaatt gaactataat gtagctccaa caaatattgt ggctgctgta gttgccaaag
     9121 atgattggcg ttatctgggt tttttccgtt ggggtttaat tccttcctgg agcaaaaaaa
     9181 ttccggaaca ggcactaatt aatgttcgca gtgaaagcat cttagaaaaa ccgagtttca
     9241 agacatcttt tttgcgcagg cgatgtttaa ttcctgccaa tggattttat gaatggcgta
     9301 aaacagataa gcaacccttt tttattaagg caaaagggga taatttgctt tatcttgccg
     9361 ggatttatga tgcctggtat ggacccgacg gcagttatat tccttctttg ggaataatta
     9421 ctacttctgc caatgatttt attcagccct tgcacgaaag aatgcccctt ttgctaaatc
     9481 cttcattata tgatacctgg ctgaatcctg cagcacaaaa tccccaagaa ctccaattgc
     9541 tgcttacagt tccctcggaa attgaactgg aaatgtatcc agtcagcaga agagtgaata
     9601 aacctgaaaa caatgatgcc gattgcttaa aaccgattgc gctttaggca gggcaaaatc
     9661 atttttataa ggcaccgaat atggataact taaaaaaaca aattctgatt gtagatgacg
     9721 atttagctat tctggattgc atccgaatga gcttacaaaa ggattttccc tgtaggataa
     9781 ctacctgcag tgattcccgg gaagcattgg aactgctgga aagcaatatt ttcaatgtga
     9841 ttatttccga tattggaatg cccaatcttt ccggatttca gcttttggaa tatatcaata
     9901 aactgaatcc taatatccct gttattctga taacaggtga agtagacacg gaaaaaatac
     9961 gctctgccgt tcagttaggt gcctttgata ttctccataa accctttgaa ttatccgatt
    10021 tacatatttc agttaaacag gcattacaga aaaattccct ccttctccaa aatgaaatgt
    10081 atcgcttaaa tctggaagaa ctggtggaaa aaagaacaaa ggaactttat attgcccaat
    10141 gtcaactgga aaagaattat ttaaatacca ttcatgcaat ggttaatgca atggaagcca
    10201 atgatgttta tattcgtgga cattccgaaa gagttactgt cttaagcatt atgctgggta
    10261 aaattatcgg gctggatgcg gaagacctta aacttttaag aataggtgct atccttcatg
    10321 atttaggcaa aattggcatt tacgacaccc ttctcaataa aaaagggtct ttaacctcca
    10381 gtgaatatga aatggtaaaa cagcatccgg ttatcggtgc taaaattatt cgccccattg
    10441 gattgccaac tcccgtgtat gaaattattc tccaacacca cgaatggttt aacggcaagg
    10501 gctatcctta tggcttaacc ggtaaccaaa tttctccttt agccagaatt gttactattg
    10561 cagatgctta tgatgctatg accagtaaaa gagcttaccg cgaaaatctg gatgccaaat
    10621 ctgccagaca ggaagtgatt aataatgcag gaattcagtt tgatcctgag tatggcaagg
    10681 tcttctttga taattattct catattgttg agcccgtgag tgatagtccc gcaattcaga
    10741 acctgctgtt tgatatgtta taaagataat tagaaagtat ataaggagtt ataatggtac
    10801 aaattattag tgccgcagag gcagctgcaa tgattaaacc cggaaaccgt cttgccattg
    10861 gcggtttttt agctgtcgga gctccggaga aaatcattga cgcaatggta gaaaacggaa
    10921 ataaagacct ccacataatc gttattgctt ccgactggga agacagaggt gtgggtaaac
    10981 ttgttgtaaa taagatggtt aaaagtgctc aggtttcgca tatgggaacc aataagacaa
    11041 ttcaagccca aatgaatgcc ggcgaaattg atattgaact tgttcctcaa gggacactta
    11101 tggaaagagt tcgcgctttt ggagccggtt tagggggtat tttaactcct acaggactgg
    11161 ggacaattgt ggctgaaggg aaacaaatta ttactgtgga cggaaaggat tatcttctgg
    11221 aaactgctat tcccagcgat tttgctctca ttaaagctca caaagccgat aaattaggaa
    11281 acttaactta ccgcaaaaca gcccgtaaca gcaatcctat tatggctatg gcaggtaaaa
    11341 ttaccatcgc ggaagttgac gaaattgtgg aaattggcga actggaccct gaggaagtaa
    11401 taactcctgg tgtttttgta aattatcttg ttttagcaaa ggagaattaa gatggcatta
    11461 gataaacgag ctatgatcgg agcacgcatt gctaaagaat tcaaagatgg cgattatgta
    11521 aatttgggta ttggtttacc tactgaagct gcaaatcaca ttccggaagg tattagcgtt
    11581 atcttccagt cggaaaacgg acttttaggt gccggtccaa aacctcctga aggacaggaa
    11641 gataaagata tgatcaatgc cggaggaggt tacataacct gtattccggg agcaagtttt
    11701 tttgattctg ccacctcttt tgctatcatt cggggtggac atatagatgc caccgtttta
    11761 ggagctctgc aagttgacca ggaaggaaat ttggctaatt ggttaattcc tggaaaaatg
    11821 gttcccggaa tgggaggagc tatggatttg gtgactggag caaaaaaagt tatcgtagca
    11881 atggaacact gtgataaata tggcaactcc aaaatcctga aaaaatgcac tttaccgctt
    11941 accgctaaag ggaaggtaaa cctcattatt accgatatgg ctgtgataga agtttgccct
    12001 gagggacttt atttacgcga aattgccgaa ggacttacca ttgatgatgt agtgcaagct
    12061 accgaagcag aactgattat ccctgaaaga gttaatacct tctgataaca aaaaaagttg
    12121 agaaggttga gagggtttag agggtttaga aggttgagat attaaaagcc cgaagggcga
    12181 tacaatgata gcgaaggtgg cgtaagcccc tcgttaaaaa ataggaaaac actctctgct
    12241 cttcgcactt tgctctttgc caaaaagccc gaagggcgac acaatgatgt agaaggttga
    12301 gatttccgga aggttgatat ccttgtaaac ccctcgtaaa aatttaagtc agga
//
LOCUS       DAOPFL010000048        12337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_6104_length_12337_cov_102.797427, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000048 DAOPFL010000000
VERSION     DAOPFL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6104_length_12337_cov_102.797427"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..703
                     /gene="plsY"
                     /locus_tag="PLG69_04750"
     CDS             71..703
                     /gene="plsY"
                     /locus_tag="PLG69_04750"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPI42853.1"
                     /translation="MSFNNFLPYIILLFAYLLGSIPIGLIVGKVFYHKDIRKEGSGNI
                     GATNALRAFGTSTGLIVLLLDMAKGFIPVMLATSFLEKGSPFIALTALLVILGHIFPL
                     YLGFKGGKGVATASGAFLALTPLSLLIALIAFIIVVAVFRYVSLASICAAFVFQWHCF
                     ALLLKEAKPDYATVILVTIVVLMIISKHKSNLERLLNKTEPKLKFTKKGN"
     gene            713..2095
                     /gene="purF"
                     /locus_tag="PLG69_04755"
     CDS             713..2095
                     /gene="purF"
                     /locus_tag="PLG69_04755"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPI42854.1"
                     /translation="MCGIIGVFGNDNAGRLAAVGLFAEQHRGQESCGMAVSDGLLIRL
                     HKKMGLVKEVFHEEQLNALPGKIAIGHVRYPTKGSATEFNTQPHLVETLSGPCYALAS
                     NGDIVNYAQVRKMLENEKVYFKSDNDAELLVKYIAYRILHYKENIGDAIRNLMRDIKG
                     AYSSVLCTPTELYMFRDPYSIRPMIWGKTPEGTVIVASESCALDTLGVQDRKEVPPAG
                     IIKVSDSKIEVLDNDPNLYREGNCEKHCIFEQIYFSRPDSFHFGENVYAVREKIGAAL
                     ALQDEGLNPDLVVPVPDSSNFIGLGYANQNGIPLSLGLIRNHYIGRTFIKPEQAVRDE
                     SVRQKFNVLPNFFTGKRIVLIDDSIVRGTTIRKIVRLIKDAGAAEIHLRIGSPQIKHS
                     CYYGIDTPTTEELIANRRTLEEIREITGVDSLKHLPLNDLKTCVHCPENYCYACFDGN
                     YPVGVPDVCN"
     gene            2236..3225
                     /gene="rfaE1"
                     /locus_tag="PLG69_04760"
     CDS             2236..3225
                     /gene="rfaE1"
                     /locus_tag="PLG69_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPI42855.1"
                     /translation="MYSIKPEKLEEILKSFQNRRIIVLGDIMLDEYLWGKVQRISPEA
                     PVPIIEVNSTDYRLGGAANVAMNLSVLNAKVDLVGVCGKDTQAEIIVQQLKKREIPVE
                     GIFFDPSRPTTLKTRIGSVSQQIVRLDREEVAEIDNSIQKEIFNYLVKIIPLCDALLI
                     EDYNKGLLSFNLITDVLQLALKHKKMVAVDPKYSNFSSYGGVEIFKPNYRELESYMGR
                     KLTDQDDFERSTEELRQRMSIKHLVVTKGSEGMYLFSENKPVNHLPSFAREVFDVSGA
                     GDTVISALTLAYLYDRNIETAALIANHSAAVAVAKKGTASVSIEEIWDSFNAP"
     gene            3215..3694
                     /gene="rfaE2"
                     /locus_tag="PLG69_04765"
     CDS             3215..3694
                     /gene="rfaE2"
                     /locus_tag="PLG69_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPI42856.1"
                     /translation="MLHNKIIPYSEIAELSQKLHQQGKKIVFTNGCFDILHCGHILYL
                     EKAKALGDILILGLNSDASVKRLKGNNRPIVPEKERALVVAGLESVDYVCIFAQDTPY
                     ELIDLIQPDVLVKGGDWTIDKIVGADIVQKKGGKVLSLNYEQGFSTTNIIERIKQDK"
     gene            3696..4106
                     /locus_tag="PLG69_04770"
     CDS             3696..4106
                     /locus_tag="PLG69_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPI42857.1"
                     /translation="MQEEIIEDSGIVKKVEGNSIIVEIERGGGCNSCKMQGFCFKKNE
                     PAEFEIVTDIPLQVNDRVQLDISPLGRTLVSLLIFGVPLLFLFLGFLIASLWFTEIVS
                     ILIGFTAMALSFRIIRFIDTKYGKRLKISIARKL"
     gene            4103..4486
                     /gene="folB"
                     /locus_tag="PLG69_04775"
     CDS             4103..4486
                     /gene="folB"
                     /locus_tag="PLG69_04775"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPI42858.1"
                     /translation="MKIRLNEMVFYGFHGVNAEERSLGQRFVVSVVLHTNPTIDKTIR
                     RLEDTIDYTKIYAEIKEIMESRQFYLLEDCANTIADKLLADFPLLEKLTICIQKPSVP
                     IPGSLKSVEVILSRTRNNQEDNLSQ"
     gene            4483..4986
                     /gene="folK"
                     /locus_tag="PLG69_04780"
     CDS             4483..4986
                     /gene="folK"
                     /locus_tag="PLG69_04780"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPI42859.1"
                     /translation="MIYYLCLGSNLNNPVSQLDGAIQHLENDPGIKILRSSKRIITEP
                     YGIINQPDFLNQVLEVESSYQPQELMERLLNIEKAMGRIRRIKWGPRNIDIDILFAGD
                     LVLDSRITPLEKSSPEVIIPHPDLHNREFALRLLNELIPNAKHPLMHKTIYELYYSIV
                     AEEEYYE"
     gene            4979..6007
                     /locus_tag="PLG69_04785"
     CDS             4979..6007
                     /locus_tag="PLG69_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPI42860.1"
                     /translation="MNNLTGIILAAGKGTRMKSERAKVTLPLADKPMIQRVVETALSA
                     NCQKIYIVVGYMKNNVISAVEDNEIIEFVEQEEQLGTGHAVMITEPLFTNPDQDVLIL
                     CGDVPLLSAQTLIRIYEKHKSSSAACTVLTAFLDDPGKYGRILRDTSGKICGIKEYKD
                     ASEEQRKIKEWNTGIYCFQVKNLFSALKQTSNHNQQSEYYLTDTIEILYRQGKTISNV
                     VLEDLMEVSGVNSQEELAALEDIYVDRIRKKWLNNGVVIHNPHSVYIGDEVIIEPDVE
                     IHQNTVIKGKSTLEKGCVIGPSCYLEHSTVSLDSLLQGHNILVNAILPEHHILDFGSH
                     IIEEIRYE"
     gene            6000..6521
                     /locus_tag="PLG69_04790"
     CDS             6000..6521
                     /locus_tag="PLG69_04790"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPI42861.1"
                     /translation="MNNNKKSKDEHHYLYSPWRLDYILGEKDGDCIMCRFQNTDNDKE
                     NLILHRAENCYVMLNRYPYNNGHLMIIPYQHCGALTELQEEAWLEMAKLVRNTEAILK
                     KVYNCDGINIGINLGSAAGAGIAEHLHIHIVPRWVGDSNFMSVIGGERVIPESFDSVY
                     NKLAPEFAKLIKN"
     gene            6590..6982
                     /locus_tag="PLG69_04795"
     CDS             6590..6982
                     /locus_tag="PLG69_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPI42862.1"
                     /translation="MPITLILIAIVAIVLFFIWKKNTEESKPVYSEEQLPAIKVVVTN
                     GCGYERLAADFAAALKDKNIEVVGLSETPKPIYDKTIIVIRKGDKEDLERLMKMTGIQ
                     RWTSAYSEYFSADFEIIVGRDYEQFIAY"
     gene            6992..7348
                     /gene="rsfS"
                     /locus_tag="PLG69_04800"
     CDS             6992..7348
                     /gene="rsfS"
                     /locus_tag="PLG69_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPI42863.1"
                     /translation="MLAEYISLEVIISWLKEKKAENIRIYDVQEKCDYTDIVVVCEGS
                     ADVHNKAIANYMVEMAKKNNLRILSKEGIDYGHWILLDIGDLIVHIFLPETRDYYNID
                     ELLAKIAKIPVQENER"
     gene            7345..8994
                     /gene="argS"
                     /locus_tag="PLG69_04805"
     CDS             7345..8994
                     /gene="argS"
                     /locus_tag="PLG69_04805"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPI42864.1"
                     /translation="MIKELLHKNIVEALDNLKLNHEKEFTVEIPNNPEFGDYSTNAAL
                     VLGKENKMKPKDLAEKIVKELKKNKAYKKLEIAGPGFINFFLSPSLYQQVFWEIYKSE
                     DYGKSDFGNQEKILLEFVSANPTGPLNIVSARAAAFGDTLYRIMKYVGYAPAREFYIN
                     DAGNQVDILAESLELRLREIHGEKIGEFPYEAYHGEYVKHLAHRLNALEGTRVFMLPQ
                     KDRLEHLKEFALSELLEMQRNSLEKFGVFFEGWVSEKALRAEGVVEEVLSYLTEADCT
                     YEKEDAIWFNSTKYGDDKDRVLMKSDGSITYFVPDVAYHLTKIQRGFTKLIDIFGPDH
                     HGYVPRIRAAFRALNYDDSILEFIFLQQVNIFESGERVKMSKRAGKIVTMDDLISVVG
                     KDAARYFFIARKANAHLNFDLELALKKNNENPVYYCQYAHARICSIIKKARSEKIYPK
                     HFKKEMIKKLNKPEELALIQKMADLPELLILIAIHREPHRLTTYIEELASLLHHYYEK
                     YQIVNVKNPALSQARLMLLYTVRKVMQICFELMGISAPETM"
     gene            9012..9464
                     /gene="rimI"
                     /locus_tag="PLG69_04810"
     CDS             9012..9464
                     /gene="rimI"
                     /locus_tag="PLG69_04810"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPI42865.1"
                     /translation="MDNAYSIRLSCEDDFDAIEEIEHQVYSNPWPPEAFSRMLFPWAF
                     TLLYGKEVIGYIFYCGVKEEMVIVNFAVKPDFQGKGLGEYLLTETMRMMNNNGVQRFY
                     LDVRISNYKARRLYEKVGFQEIGIRKQYYNNPDEDALVMGMEFNDIRV"
     gene            9448..11049
                     /gene="nadB"
                     /locus_tag="PLG69_04815"
     CDS             9448..11049
                     /gene="nadB"
                     /locus_tag="PLG69_04815"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPI42866.1"
                     /translation="MTYEYDYLVIGSGIAGLIYALQVSKYGKVAVVTKSSLNDCNTDH
                     AQGGIAAAIDSLDSFEAHIEDTYQAGAGLGKRQVITEVISSGPKLIQYLIDLGTDFTR
                     RDPNYDICLENLSLTMEGGHTRRRIAYAADSTGHQIMETLIAQCQKNPNIEIFEYYIA
                     IDLITQHHVVQDEGFIPGISCWGAYVLNINENRVDIFKARKTMMATGGAAQIYSPDTN
                     PKVATGDGMAMARLAGARLANMEFVQFHPTAFWSPDGDTFLISESLRGEGAVLRLSDG
                     STFMENYHPQGNLAPRDIVSRAIDSELKKRGEKFCYLDATIVPAEQLLRHFPSINNML
                     KARGIDFTKEPIPVAPAAHYFCGGVLSTIEGITDIRNLFVAGEVACSGLHGANRLASN
                     SLLEALVMAYKAGNHPSNLEEVQFPCIPEWKVIDEFNENEWVVISHNREIIGTIMQGY
                     AGIRRSRRLLKYALSRLENIYNEINNFYQHNAVRKEVVETRNMAIIAIAVVKSALMRK
                     ESRGAHFLVDNPERDDEHYKHDTII"
     gene            11060..11749
                     /gene="tmk"
                     /locus_tag="PLG69_04820"
     CDS             11060..11749
                     /gene="tmk"
                     /locus_tag="PLG69_04820"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPI42867.1"
                     /translation="MHNKSSHCVSSHLENLTPKDSFSGKGLFITFEGIEGSGKTTQII
                     MLVEVLKAKGIPYVTTREPGGTKISEAIRDLLLNPEFMEMKPETELLLYCASRAQHTA
                     ELILPALEEGKIVISDRYFDSTYAYQGAARELDEEVINVLTRFSTYGRNPDLTFLLDL
                     PVEVGLARIKNRNLDRLEQETLSFHEKVRRQFLSIANKYPSRYIVLNGESQPEIIHRQ
                     ILSTLQPFIGE"
     gene            11751..>12337
                     /locus_tag="PLG69_04825"
     CDS             11751..>12337
                     /locus_tag="PLG69_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPI42868.1"
                     /translation="MNRPKQKLALISIISIWVLSALLLIVATNAYAQNRPQSTNLYSQ
                     VQLFSEVLYKLKQYYVTDLNDEELIKAAIDGMLSSTDPHTTYFTPEEFKEFTTTTKGS
                     FGGLGIQIDKIGNYITVVSPIEGTPAYRMGITAGDRIIKVDDVSIVGFSTDEAVKRMR
                     GDVGTKVKITISRPGLPEPIDFELTRETIKIKSIP"
BASE COUNT         3930 a         2116 c         2619 g         3672 t
ORIGIN      
        1 gaaatgttca aatttgaagg agttagtatc aaactcattt ttaagggacg caaaaaaggt
       61 gaggataaac ttgagcttca ataattttct tccctatatt attttacttt ttgcctatct
      121 tttaggaagt attcctattg gtttaattgt aggtaaggtc ttttatcata aggatatccg
      181 caaagaaggc agtggcaata ttggtgctac taatgcttta agagcttttg gaacttctac
      241 gggacttatt gttctgctgt tagatatggc aaagggtttt attcctgtta tgttagctac
      301 atcgtttctg gaaaagggca gtccttttat tgccttaact gcgcttttag ttattttggg
      361 tcatatattt ccgctatatt taggttttaa aggcggtaaa ggtgtagcta cagcaagtgg
      421 agctttttta gcattaaccc ctttaagctt gcttatagca ttgattgcct ttataatagt
      481 tgtagctgtt ttccgttatg tatctctggc ttctatttgc gctgctttcg tctttcagtg
      541 gcactgtttt gctttattac tgaaagaggc aaaaccggat tatgctactg tgattttggt
      601 aacgatagtg gttttgatga ttatcagcaa acataaaagc aacctggaac gcctgctgaa
      661 caaaacagaa cccaaactga aatttacgaa aaaaggaaat tagaacaaaa aaatgtgtgg
      721 aataattggc gttttcggaa acgataacgc gggacgactc gctgctgtag gactttttgc
      781 ggaacaacat agaggacagg aaagttgtgg aatggcagtt agtgatggtt tgctaattcg
      841 tttgcacaaa aaaatggggc tggtaaaaga agtttttcac gaagaacagc ttaatgcctt
      901 acccggaaaa atagctattg gtcatgtgcg ttatcctaca aaaggcagcg ctacggaatt
      961 taatacccaa ccccatttag tggaaaccct ttcaggacct tgttatgctc ttgcctctaa
     1021 tggagatatt gtaaactatg ctcaagtgcg aaagatgctg gaaaatgaga aggtctattt
     1081 taaaagcgac aacgatgccg aattgcttgt taaatatatt gcctaccgta tcttacacta
     1141 caaagaaaat atcggagatg ctatccgaaa tttgatgagg gatattaaag gtgcctattc
     1201 cagtgtatta tgcactccta cagaacttta tatgtttcgt gatccctata gcatccgacc
     1261 tatgatttgg ggtaaaacac ctgaaggaac tgtaatagtt gcctcagaaa gctgtgcttt
     1321 ggacaccttg ggagtgcagg atagaaaaga agttcctcct gctggaatta ttaaagtaag
     1381 tgatagcaaa atagaggtgt tagataatga ccccaatctc tatcgggaag gcaattgtga
     1441 aaaacactgc atctttgaac aaatatattt ttcccgtccc gatagttttc acttcgggga
     1501 aaatgtttat gctgtgcgcg aaaaaatcgg ggctgctctt gctcttcaag atgaaggact
     1561 aaatcctgat ttggtagttc ccgttcctga ttcatcaaat ttcatcggtt tgggttatgc
     1621 caatcaaaac ggcatccctc tttctttggg cttaattcgt aatcactata ttggcagaac
     1681 ttttatcaaa ccggaacagg cagtccgcga tgaaagcgtg cgccaaaaat ttaatgtcct
     1741 ccctaatttc tttaccggaa aaagaattgt gctgattgat gacagtattg tgcgcggaac
     1801 tactattcgt aaaatcgtga gattaattaa agatgctggc gctgcagaaa ttcacttaag
     1861 aattggcagt ccccaaataa aacacagctg ctattatgga atagatactc ctactacaga
     1921 agaattgatc gccaatcgta gaacccttga agaaattaga gaaattaccg gtgtagatag
     1981 tttaaaacac ttaccattaa atgatttgaa aacctgtgtg cactgcccgg aaaattattg
     2041 ttatgcttgt tttgacggga attatcctgt aggagttcct gatgtatgca attgaagcag
     2101 gaaggttgat gtctccgtca actacttctt acaattcatt tcactcccca aaagtttact
     2161 taaccgaaaa aggtaaggaa accaaaagaa cgacgaggat gtcgttcttc ctgcccttta
     2221 taataggagt tattgatgta ttcaattaaa cctgaaaaac ttgaagaaat cctgaaaagt
     2281 tttcaaaatc gccggattat agttcttggc gatattatgc tggatgaata tctgtgggga
     2341 aaagttcaac gtatttcccc tgaagcccct gttcccatta tagaagtaaa ttctaccgat
     2401 taccgtttgg gtggagctgc taatgtagct atgaatctta gcgtgctgaa tgctaaagtt
     2461 gatttggtgg gtgtttgcgg aaaagatact caagccgaaa ttatagttca acagttgaag
     2521 aaaagagaaa tacccgttga aggaattttc tttgatcctt ctcgtcctac aactttaaaa
     2581 accagaattg gttcggtcag tcaacaaata gtgcgtttgg atagagaaga agttgcagaa
     2641 atagataaca gcatccaaaa agaaattttt aactatctgg taaaaatcat accattgtgc
     2701 gatgctcttc taattgagga ctacaataaa ggtctgctga gtttcaattt aataaccgat
     2761 gttttacaac tggcattgaa acataaaaaa atggtagctg tagaccccaa atactcaaat
     2821 ttcagctcct atggcggtgt ggaaattttc aagccaaact accgtgaact ggaaagttat
     2881 atgggcagga aacttaccga tcaggatgac tttgaaaggt ctacggaaga attgcgacaa
     2941 agaatgagca tcaaacattt agtagtaacc aaaggcagtg aaggaatgta tcttttttcc
     3001 gaaaacaaac cggttaacca tcttcccagt tttgcgcgcg aggtttttga tgtttccgga
     3061 gcaggagata ccgttatcag tgctttaact ttagcttatc tttatgatcg gaacattgaa
     3121 actgccgctt taatagccaa tcattctgct gctgtagctg tagctaaaaa aggcactgcc
     3181 agcgttagta ttgaagagat ttgggatagt tttaatgctc cataataaaa tcattcccta
     3241 ttctgaaata gcagaattaa gccaaaaact acatcaacaa ggaaaaaaga ttgttttcac
     3301 caatggctgt tttgatattt tacattgcgg tcatatcctc tatctggaaa aagcaaaggc
     3361 attgggagat attcttattt tgggtctgaa tagtgatgct tcagtaaaac gactaaaggg
     3421 aaataaccgt cctattgttc ccgaaaagga aagagccctg gtggttgcag gtttggaaag
     3481 tgtggattat gtttgtattt ttgcacagga cactccttat gaactaatag accttattca
     3541 acccgatgtt ttggttaaag gtggtgactg gactattgac aaaattgtcg gtgccgatat
     3601 tgtgcaaaaa aaaggtggca aagtcctttc cctgaattat gaacagggtt ttagcactac
     3661 taatataatt gaacgcataa aacaggataa ataaaatgca ggaagaaata attgaagata
     3721 gcgggatcgt aaaaaaggta gaaggaaata gtattatagt ggaaattgaa cgcggaggcg
     3781 gttgtaacag ttgtaaaatg caaggttttt gtttcaaaaa gaatgaaccg gctgaatttg
     3841 aaatagttac agatatcccg ttacaagtaa atgatagagt gcaattggat atttcgcctt
     3901 tagggagaac tctggtttca ctattgattt tcggagtgcc tttgctgttt ttgtttttgg
     3961 gctttttaat tgcctctttg tggtttactg aaattgtttc cattttaatc ggttttaccg
     4021 ctatggcact ttccttcagg ataatacggt tcatagatac aaaatatggc aagcggttaa
     4081 aaataagcat tgcgaggaaa ctttgaaaat aagattgaat gaaatggtct tttacggctt
     4141 tcatggagtg aatgctgaag aacgctcttt gggacagcgt tttgttgtta gcgttgttct
     4201 gcataccaat ccgaccatag ataaaactat tcgccgtttg gaagatacta ttgattacac
     4261 caagatctat gcagaaataa aggaaattat ggaaagccgg caattctatt tgctggaaga
     4321 ttgtgccaat actattgccg ataaactatt ggctgatttt cctctgctgg aaaagctgac
     4381 tatctgcatt caaaaaccct cagttcctat tccaggaagt ttgaaatcgg tggaagtgat
     4441 attaagccga accagaaaca atcaggagga taacctgtct caatgattta ttatttatgc
     4501 cttggttcca atttgaataa tccggtaagt cagcttgatg gagcaattca gcatttggaa
     4561 aatgacccag gaataaaaat tctccgcagt tctaaaagaa ttataaccga gccctacgga
     4621 ataattaatc aacctgattt tcttaaccag gtgctggaag tggaaagcag ttatcagccc
     4681 caagaattga tggaaagatt gctaaatatt gaaaaagcta tgggtagaat aagaaggata
     4741 aaatggggac cgcgtaatat tgatattgat attctattcg ccggagacct ggttttggat
     4801 agtagaataa cacctttgga aaagtcatcc cctgaagtta ttattcccca tcccgattta
     4861 cataatcgtg aatttgcttt gcgcttgctg aatgaactga ttcctaatgc caaacatcct
     4921 ttaatgcata aaacgattta tgaattatat tatagtatag tagccgagga ggaatattat
     4981 gaataatctg acagggatta ttcttgctgc aggaaaaggc acgcgtatga aatccgaaag
     5041 agctaaagta actttaccgc ttgccgataa accaatgatt caaagagtag tggaaacagc
     5101 tttatccgct aattgccaaa aaatatatat agtcgttggc tatatgaaaa ataatgtaat
     5161 ttctgctgta gaagataatg aaataattga atttgtggaa caggaagaac aactgggaac
     5221 aggtcatgcg gtaatgatta cagaaccctt attcacaaac cccgatcagg atgtgctgat
     5281 tttatgtgga gatgttcctt tgctttctgc acaaaccctt atccggattt atgagaaaca
     5341 taaaagttct tcagctgctt gcacagttct aactgccttt ttggatgacc caggaaaata
     5401 tggacgcatc ttaagagata catcaggcaa aatttgcggt atcaaagaat acaaagatgc
     5461 cagcgaagaa caaagaaaaa ttaaggaatg gaatacaggt atctattgtt ttcaggtaaa
     5521 gaatttattt agcgctctca aacagacctc caatcacaac cagcagagtg aatattatct
     5581 tacagacact atagaaatcc tttatagaca aggcaaaacc atttccaatg tggttctgga
     5641 ggatttaatg gaggtttcag gagtaaattc acaggaagaa ctagctgcct tagaagatat
     5701 ttatgttgat cgtattcgga aaaaatggct caataacgga gttgttattc acaatccaca
     5761 tagtgtttat attggagatg aagtaataat tgaaccggat gtggaaattc atcaaaatac
     5821 ggttattaaa gggaaaagta ctttggaaaa gggttgtgtg atcggtccct cttgttattt
     5881 ggaacacagc actgtttctc ttgattccct cctgcagggt cataacattt tagtaaacgc
     5941 tattctgccg gaacatcata tcctggattt cggttcgcat ataattgaag agataaggta
     6001 tgaataacaa taagaaaagc aaagatgaac atcactatct ctattctccc tggcgattgg
     6061 attacatttt aggggaaaaa gacggtgatt gtataatgtg ccgttttcaa aatactgata
     6121 acgataaaga aaacttgatc ttacatcggg ctgagaattg ttatgtgatg ttgaatcgct
     6181 atccttacaa taacggtcat ttaatgatta ttccttatca acattgtgga gctctaaccg
     6241 aattacaaga agaggcctgg ctggaaatgg caaaactcgt cagaaatact gaagccattt
     6301 tgaaaaaggt ctataactgt gatggcataa atatcggcat caatcttggt tctgctgctg
     6361 gagcaggaat tgctgaacac ttgcatattc atatagttcc acgctgggtt ggagatagta
     6421 attttatgag tgtgataggt ggtgaaagag taattccgga atcctttgat tctgtctata
     6481 acaaactggc acctgaattt gccaaactta taaaaaatta agaggttatc ggttgaattt
     6541 gacaaacaac ccgcctgaaa ataagttgac cacaaaaaga aagatttggt tgcctataac
     6601 cctaattctt attgctattg tggcaattgt gctttttttt atctggaaga aaaacactga
     6661 ggaaagtaaa cctgtataca gcgaagaaca gcttccggca attaaagtag tggtaactaa
     6721 cggttgtggt tatgaacgct tggctgcaga ttttgctgcc gctcttaagg ataaaaatat
     6781 agaagtggtt ggtttgtcag aaacccccaa accaatatat gataagacga taatcgttat
     6841 tcgcaaagga gataaggaag atttggaacg gttgatgaaa atgaccggaa ttcaaagatg
     6901 gacaagtgct tacagtgaat atttcagtgc cgattttgaa atcattgttg gcagagatta
     6961 tgagcagttt attgcttatt gaagggagga tatgttggcg gaatatatta gtttggaagt
     7021 aattattagc tggctgaaag agaaaaaagc agaaaatata cgcatttatg atgttcaaga
     7081 aaaatgtgat tatacagaca tcgtggttgt ttgcgaaggc agtgctgatg tgcataataa
     7141 agctatcgcc aattatatgg tggaaatggc aaaaaagaat aacctgcgta ttttaagtaa
     7201 agaaggcatt gattatggac attggattct gcttgatatt ggagatttaa ttgtgcatat
     7261 ttttttaccc gaaacaaggg actattataa tatagatgag cttttggcaa aaatagccaa
     7321 aatacctgtt caagagaatg agagatgatt aaagaacttt tacataagaa catagttgag
     7381 gcattggata atctgaaact caatcacgaa aaggaattta ctgtagaaat tcccaataat
     7441 ccagagtttg gagattattc taccaatgcc gctttggttt taggtaaaga aaacaagatg
     7501 aaaccaaaag atttggcaga aaaaatagta aaagaactga aaaaaaacaa agcatataaa
     7561 aaactggaaa ttgccggacc gggtttcata aacttctttc tttcaccttc cctgtatcag
     7621 caggtttttt gggaaattta caaaagtgag gactatggta aaagtgattt cggcaaccag
     7681 gaaaaaatcc tgctggaatt tgtaagtgcc aatcctacag gtcctttaaa tatagttagt
     7741 gccagggcag ctgcttttgg agatacacta tatagaataa tgaaatatgt gggctacgct
     7801 ccggcaaggg aattttatat taacgatgct ggaaatcagg ttgatatttt agctgaatcg
     7861 ttggaattgc gtttacggga aatccatggc gagaaaatag gtgaattccc ttatgaggca
     7921 tatcatggag aatatgttaa acatctggct caccgtttga atgctttgga agggacaagg
     7981 gtttttatgt tgccgcaaaa agaccgttta gaacacttga aggaatttgc tctttccgaa
     8041 ctgctggaaa tgcaaagaaa ttcccttgaa aaatttggtg ttttttttga gggttgggtt
     8101 tccgaaaaag ctctacgcgc agaaggtgta gtagaggaag ttctttccta tttaaccgaa
     8161 gcggattgca cttatgaaaa agaagatgct atctggttta acagcacaaa atatggtgac
     8221 gataaagata gagtgctgat gaaatcggat ggttcaatta cctattttgt gcctgatgtt
     8281 gcctatcatt taacaaaaat ccagcgtggt ttcaccaagt taattgatat cttcggtcct
     8341 gatcatcatg gttatgttcc gcgtatcaga gctgcctttc gtgctttgaa ctatgatgat
     8401 tccatattgg aatttatttt tctccagcag gttaatatct ttgaaagtgg cgaaagggtt
     8461 aaaatgagta aacgagccgg taaaatcgtt actatggatg accttattag tgtggtaggc
     8521 aaagatgccg cgcgctattt tttcattgcc cgcaaagcta atgcccactt aaattttgat
     8581 ttggaactgg cactgaagaa aaacaacgaa aatcctgttt attactgtca atatgcgcat
     8641 gcccgcattt gcagtattat caaaaaagcc cgcagtgaga agatttaccc caagcacttc
     8701 aagaaagaaa tgataaaaaa gctgaataaa ccggaggaac tggcactgat tcaaaagatg
     8761 gctgatttgc ctgaattgct tattttgata gctatccacc gggaacctca tcgcttaact
     8821 acttacattg aagagctggc gtctttactg caccattatt atgaaaagta tcaaatcgta
     8881 aatgttaaaa atcctgctct ttctcaagca cgattgatgc tgctatatac ggttagaaaa
     8941 gttatgcaaa tctgctttga gttaatgggc atcagtgctc ctgagacgat gtaaaaaata
     9001 aagtgaagat aatggataat gcttatagca ttcgtttatc ctgtgaggat gattttgatg
     9061 ccatagagga aatagaacat caggtatatt ccaatccctg gcctcccgaa gctttttccc
     9121 gtatgctttt tccttgggct tttactttac tttatggcaa ggaagttata ggttacatct
     9181 tttattgcgg tgtaaaagaa gaaatggtta tcgtaaattt tgccgttaaa cctgattttc
     9241 agggtaaggg cttaggagaa tatctgctca cggaaacaat gcggatgatg aataataatg
     9301 gcgttcaacg cttttatctg gatgtgagaa tatccaacta taaagcgcgt cgtctttacg
     9361 aaaaggtagg atttcaagag attggaatcc gcaagcaata ttacaacaat cctgatgagg
     9421 atgctttagt tatgggaatg gaattcaatg acatacgagt atgactattt agttatcggt
     9481 agtggtattg ccggtttaat ttatgctctg caagtttcca aatatggcaa agtggcagtg
     9541 gtaactaaaa gttcactaaa tgactgtaat acagaccacg ctcaaggggg aattgctgct
     9601 gccatagatt ctctggattc ctttgaggca catattgagg atacttatca ggcaggagca
     9661 ggattgggta agcgtcaggt tattactgaa gtaatttctt caggacccaa actgattcag
     9721 tatcttatag atttgggtac ggattttacg cgtcgtgatc ctaattacga tatctgcctg
     9781 gaaaatcttt ccctcacaat ggaagggggt cacacacgca gaagaattgc ttatgcagct
     9841 gattctacag gccatcagat tatggaaaca ttaattgctc aatgccagaa aaatcccaat
     9901 attgaaatct ttgaatatta tatcgctata gaccttataa cacagcacca tgtagttcag
     9961 gacgaaggtt ttattccggg aatttcctgc tggggtgctt atgttttaaa tattaatgaa
    10021 aaccgggtgg atatttttaa agcccgaaaa acaatgatgg ctacaggtgg tgcagctcaa
    10081 atctattctc cggatacaaa tcccaaagtg gcaacaggtg atggaatggc tatggctcgt
    10141 ttagcaggag ctcgtttagc taatatggaa tttgttcagt ttcatcccac tgcgttttgg
    10201 agtccggatg gtgatacttt tttaataagt gaatcgttaa gaggagaagg agctgtttta
    10261 cgcctttccg atggtagcac ttttatggaa aattaccatc cgcagggaaa tttagctccc
    10321 agggatattg tttcccgggc aatagacagc gaactgaaaa aacgagggga aaaattctgt
    10381 tatctggatg ccactattgt gcctgcagaa cagttattac gccattttcc ctccatcaat
    10441 aatatgctga aagcccgagg aattgacttc accaaagaac caatacctgt agctccggca
    10501 gcccattatt tctgtggagg agtgctttct accatagaag gcatcaccga tatccgtaat
    10561 ttatttgttg ccggagaagt tgcctgcagt ggtttacatg gagctaaccg tttggcatcc
    10621 aattcgttac tggaagcgct ggtgatggct tataaagcgg gaaatcatcc ttccaacctg
    10681 gaagaagttc agtttccctg tattccagaa tggaaagtaa ttgatgaatt caacgaaaac
    10741 gaatgggtgg ttattagtca caaccgtgaa attattggaa ctattatgca gggttatgcc
    10801 ggtattagaa gaagccgacg gttactaaaa tatgctcttt cccgtctgga aaatatctat
    10861 aacgaaatta ataacttcta tcagcataat gcagtccgca aagaagtagt ggaaacacga
    10921 aatatggcta taattgctat tgctgttgtt aaaagtgctt taatgcgtaa agaaagccgc
    10981 ggagctcatt ttttggtgga taatcctgaa cgcgatgatg aacactataa acacgacaca
    11041 attatttagt gagagtactg tgcacaataa gagttctcac tgcgtcagtt ctcacttgga
    11101 aaatctaaca cccaaggact cattttcagg caagggtctt tttattacct ttgaaggaat
    11161 tgaaggcagc ggaaaaacaa cgcaaatcat aatgctggtg gaagtgttaa aagctaaggg
    11221 cattccttat gtaactaccc gcgaacctgg tggcacaaaa atatctgaag ccatccgtga
    11281 tttgctgcta aaccctgagt ttatggaaat gaaaccggaa acagaattgc tgctttactg
    11341 tgcaagcaga gctcaacata ctgcagaatt gattcttcct gcgctggaag aaggaaaaat
    11401 agtgatcagt gatcgctatt ttgattccac ctatgcttat cagggagctg ccagagagct
    11461 tgatgaagaa gtaataaatg ttttaaccag gttttccact tatggcagaa atcctgatct
    11521 gactttcctt ttagaccttc cagtagaagt tggacttgcc agaattaaaa accgcaattt
    11581 agaccgcttg gaacaggaaa ccctcagttt tcacgaaaaa gtgcggcgtc aatttttatc
    11641 tattgccaat aaataccctt ccagatatat tgttcttaat ggagaaagcc aaccggaaat
    11701 tattcaccgt cagattctct ccactctgca accctttata ggagaataaa atgaatagac
    11761 ctaagcagaa acttgctctg atctcaataa tcagtatctg ggtactaagt gctttactac
    11821 ttattgtagc aacgaatgct tatgcccaaa accgtcccca aagtactaat ctatattccc
    11881 aggtacagct tttcagtgaa gttctttata agctaaaaca atactatgta accgatttaa
    11941 atgatgaaga acttattaaa gctgccatag acggtatgct tagttccact gatcctcata
    12001 ctacctattt tactcctgaa gaatttaagg aatttactac aactactaaa ggttcctttg
    12061 gcggtttagg aattcaaata gataaaatag ggaactatat aaccgttgtc tctccaattg
    12121 aaggaactcc tgcttataga atgggaatta ctgccggcga tagaattatc aaggtagatg
    12181 atgtatccat agttggtttt tctaccgatg aagctgtaaa aagaatgcga ggtgatgtag
    12241 gcaccaaagt taaaattacc atcagtcgtc ccggtttacc tgaacctatt gattttgaat
    12301 taacccgtga gaccattaaa ataaagagca ttcctta
//
LOCUS       DAOPFL010000049        12014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_6330_length_12014_cov_80.821808, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000049 DAOPFL010000000
VERSION     DAOPFL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_12014_cov_80.821808"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..3416)
                     /locus_tag="PLG69_04830"
     CDS             complement(3..3416)
                     /locus_tag="PLG69_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42869.1"
                     /translation="MSKGPFMKKMAILPAIFLLLSFSLSAELIAEGNSKNSVSLLIDN
                     ESETILEYRIGEFEKNMITIEGEEWYQIRLPKEGITQDKGYPELPVFNRSIIIPDQAL
                     MAIEVFDLEFKDYPIKVAPSKGVITRDINPATIPYTFDNVYQENSFYPQKMVALSEPY
                     ILRDFRGITVLTTPFAYNPVTGTLRVYTSYKVKVYNQGNDTVNTFNRFRTSVSRSFSP
                     LYENHFLNWNSYRYTPVDDSYGKLLVICHSSFLTQIAPYINWKRQKGIDTELVEFSTI
                     GTTANQLKAYIQNRYNADNSITFVQLVGDAPQIPTLSYSGGGSDPGFSLVAGSDNYPD
                     IFIGRFSAQTTAEVTAQVNKTIQYEKDLNTSATWLSKAMGIASAEGTSGEGDNGESDI
                     THMNLIRTKLLNYGYTSVDQIYDPGASASTVTTNVNTGRGFINYVGHGSDTTWGTTGF
                     SNTNASALTNGNKVPFIMDVACVNGNFVSYTCFAEAWMRNANGGAVGIYASSINQSWA
                     SPMRAQDEVTDLLTGEVHTTLGGLYYNGSCKMMDVYGYDGVDMFKTWHIFGDASLQVR
                     SKTPLAMTVSHPASVTSGTNNIIISSGVANALVAITYNNTIYGKAVTNSSGSAIVNIS
                     NPPSGEITYTVTVTAFNRVTYTGSLQQVNVPPDQPRFVAEWEPAQGSIIRYPFGLPYS
                     LIADLSNNCLLYVIVSSSLQATCNSALVSNGVNMANVRYIVTDNDTYWIRDYGPWTIM
                     NRENNLQLIDFTYNRPRPYDDAVPLAIANFLGVDLYKMNITHTGGNIMTDGQGKAMST
                     DLVLTENTSLSQNDINIMFNNYLGVTDYQLYPDPNNTYINHIDCWAKLLDVDKVIIRS
                     VPTNHSQYNAIEAVVTQWETKLSSYGTPYKIYRVYTPNDEPYTNSFILNKHIYVPLMG
                     TANDTSAILAYQNAMPGYIVTGYLYSSFESTDAIHCRVNTIFDDKMISLKHIPPVLSP
                     VAQSLTLSANITHTNALSPSATYISWKIGKNGIWQNSNLTLQSDGNWVTNLTAPVIGD
                     TLFYCFSVTDVTNRNVTLPLCAEYDPFMLIGVYVPAISQPQVQLQVQDANVRISWNAI
                     PYANNYVIYSANSPDEVFLPVGSTSATFWETPLNTEKKFFKVVAQRVVR"
     gene            complement(3477..>3742)
                     /locus_tag="PLG69_04835"
     CDS             complement(3477..>3742)
                     /locus_tag="PLG69_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42870.1"
                     /translation="EEIPVEIEAEEKIETEEIPVEPVIPEPEHIEPEEITIIKETSEE
                     ERESEKTEKEREESETIVIPVTDETEEKKSEPLATQEETKKED"
     assembly_gap    3743..3842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3843..6222)
                     /locus_tag="PLG69_04840"
     CDS             complement(<3843..6222)
                     /locus_tag="PLG69_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPI42871.1"
                     /translation="MLIHDQNTVPPESKETENTNPEVETVLSVGEDEMSKFTVEEEIK
                     EEEPKEEEPSIAIPKEMLSENAETAVSEEAKPEEPAPEPEPEQEKKELTQEEKEHQEM
                     LSMYEESFSSFKKGEIIEGTVVDITDKDVRVDIGFKSEGIIPISEFAYTGIPELNSVI
                     KVYINEIENGEGKLLLSKKKADYMINIERIKQAFADKSSLSGIIRRRVKGGMIVDVLG
                     IEAFLPGSQMSLKPIPNLDQFIGKELYFKVVNIDEEHHNIILSRRQVLEEEAEQKRQE
                     LLRKIQIDAELDGEVKNITQYGAFIDLGGIDGLLHLTDMSWGKLNHPSEMLQIGDKVK
                     VKVINFDPETNRISLGMKQLVPHPWENIEIKYPEGSRVTGKVVNLKPFGAFVELEPGV
                     EGLVHISEMSWTKKISDAHKFLKIGETINAIVLDIDKENKRISLGMKQMEPNPWLTIE
                     DRYPIGTILTRKIKSLTAFGAFVEIEEGIDGLIHISDISWTKRIYHPREVYKKNQEVE
                     AVVLSIDRAQHRVALGVKQLVPDPWETLDQSLPINTEVKGKIGKLIPKGVLVDIPVNN
                     DVVEGFVPVSHLAIPKLEHSEDAFYIGEEIPLKVIELDMENRRLILSVKAFFFSRDPK
                     LQEEYIALHEQYMREKVARQQKKKQEKETRDKKFVQKKEEEETTKPEEKEITPPKTEL
                     IKEEIQETIGLKEEKITEPEQTITEEIQSKIEEQEETKETEKEEEIIPEIKETETIEI
                     PITEEPEPVEMPVMEEPEPIEIPVIEEPEPIEIPVIEEPEPITTEEIPVEIET"
     gene            complement(6216..7823)
                     /gene="ispH"
                     /locus_tag="PLG69_04845"
     CDS             complement(6216..7823)
                     /gene="ispH"
                     /locus_tag="PLG69_04845"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPI42872.1"
                     /translation="MPKRKIIVAIDGPAASGKSTTAKELAKKLGYVYLDTGAMYRACA
                     LQAIREGISPNDISSIVRMLEKIDIQISYTEEENIIWLNGEDVSQQIRTPEISSSASA
                     ISAIPAVRYKMVELQRKLGKNGGFVLEGRDIGTYVFPEAEAKFFMVADLEVRAKRRFK
                     ELAEKGIKTNYTDVLKELKERDEADANRSLAPLKPAEDAILIDTSNLTIEEQVHKLYE
                     ITCRIIEKIDNNNSDKEPEERKIKIRIANYLGYCFGVKRAIQMAKDAKLTEKPVYTLG
                     ELIHNPAIVQELEEQGIHIANKAEEVKNSTVIIRSHGITKEEYKILKKNNNEIIDATC
                     PYVKRTHTILKHLVKEDYPVLILGDKQHQEVIGMRSYGNEKTFVIGEDDPIPKIKEKR
                     LAIMAQTTQRIEKLQELVYKLLSNFNELRVFNTICLATTQRQKAAEELAQESDAMFVI
                     GGLKSSNTCALAKLSSVYCPTYHIEDEKQLASIDISPYKSIGITAGASTPEEMIIKVY
                     NVLLQKSGDFKTVTSIEEIPMFKEESC"
     gene            8049..10181
                     /locus_tag="PLG69_04850"
     CDS             8049..10181
                     /locus_tag="PLG69_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPI42873.1"
                     /translation="MPYKNNMKIRHYCVKCLIAIAIFAIAATNLFCYNTGDYRTKWGG
                     NFETLELWECYNGIGWIDATQLPSSPFVNTIYISNQTVTMNSSMIIEGGLVIVGTLQL
                     ASGAILTINPSVNCEIGVIETYSGSTLINNGFIKANSSSSSLKVHGGILENNGIITSS
                     APNNCNVYINSNGRINFGNRGSITGNCSFTTNYGSIIATANTQGLDGSLNCSGDISFN
                     QIYLIYNGTEPQITGMKTPDLVLGIDFNNPAGITLSKNVKLIYTALVHSGTTLYFDVH
                     IIKEAWYGSGTFSMEDGSTIATANPDGFWSTDKKGSVQVGTRNYNSNGNYIFNGTEHQ
                     QTGDFNTTPDAYTVNDIIFDNPTGVTLTHPITVVSTLELLEGDINYTVLPQGVDGFYS
                     PDVKKTVILKNGTLMYNFLADSLPFQNNGEYVKRKWYLKGNFNGSKKVTFYWSENEDD
                     NYNWNVHNFPKVYLSNSNEPLHTIWNPAHPREISFIAHSFPNAKEDVYYYIGKERDDT
                     LPVTLSSFSLTQSGISTVRITWVTQSETNIMGYYIYRATQSDLNTASNLNYLIPATNS
                     SQLQIYTYTDQVISNNIYYYWLEIVDYDGYNSFYGPAVIEINDNGNQTPSIPVKAGFY
                     SFAPNPFDSGTKIFYGIPSKSPVLMQVYNIKGQKIRTLLNETKDSGNYNLFWDGRDEK
                     GNLLANGNYILRLFASGSSWTKKITILH"
     gene            complement(10417..10866)
                     /locus_tag="PLG69_04855"
     CDS             complement(10417..10866)
                     /locus_tag="PLG69_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPI42874.1"
                     /translation="MLNLQVIYKPDELPENISQKDLVDFLYTHLESFGDAPSAINKAI
                     DYAFSAEKGKGGFLILAFAEDKLCGVLVMNKTGMEEYIPENILVYIAVEKSMRNMGIG
                     SSILKKAREIADGDIALHIEYDNPARHLYERFGFTSKYAEMRWQKKD"
     gene            complement(11198..11302)
                     /locus_tag="PLG69_04860"
     CDS             complement(11198..11302)
                     /locus_tag="PLG69_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPI42875.1"
                     /translation="MIFLVTHNPKISYSELARITGKSIATIKRLICKL"
     gene            11631..11717
                     /locus_tag="PLG69_04865"
     tRNA            11631..11717
                     /locus_tag="PLG69_04865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11665..11667,aa:Leu,seq:caa)
BASE COUNT         3526 a         2291 c         1976 g         4121 t
ORIGIN      
        1 ttttatctta caactctttg agctactact ttaaaaaact ttttctccgt gtttaaaggt
       61 gtttcccaaa aagttgcaga agtagaacca acaggcagga aaacttcatc cggtgaattt
      121 gcagaataga taacataatt attggcataa ggaattgcat tccaggatat tctaacattg
      181 gcatcctgaa cttgcaattg cacttgtggt tggctaattg caggcacata aactccgatt
      241 aacataaaag gatcgtattc cgcacaaaga ggtaaagtaa cattacgatt tgttacatcc
      301 gttaccgaaa aacaatagaa aagagtatca ccaataaccg gagccgtaag attagttacc
      361 caatttccat cactttgtaa agtcaagtta ctattttgcc agattccatt tttcccgatt
      421 ttccaagaaa tgtatgttgc acttggactt agagcattag tatgagtaat attggcactc
      481 aatgtaaggg actgagcaac gggagaaaga actggaggaa tatgcttaag agaaatcatt
      541 ttatcatcaa aaatagtgtt aaccctgcag tgaatggcat ctgtggactc aaaactgctg
      601 tataaatatc cagtaacaat ataacctggc attgcatttt gatatgcaag aatagcagaa
      661 gtatcattag cagtccccat taaaggcaca taaatatgct tattcaaaat aaaggaattg
      721 gtatagggtt catcattagg agtatagacc ctataaattt tatagggagt gccatagctg
      781 gaaagttttg tttcccattg agttactact gcttcaatag cattatattg cgaatggttt
      841 gttggcacac tgcgaataat aactttatct acatccagaa gctttgccca gcagtcaata
      901 tggtttatat atgtattatt aggatcggga tacaattgat aatcagtaac tccaagatag
      961 ttgttaaaca taatatttat atcattttga ctgagggaag tattttccgt taaaactagg
     1021 tctgtactca ttgcttttcc ctgtccatcg gtcataatat taccacctgt atgagtaata
     1081 ttcatcttat ataaatctac tcccaggaaa ttagctattg ccaaaggaac ggcatcatca
     1141 taaggacgag gacgattata ggtaaaatca atcaattgga gattattctc cctattcata
     1201 atagtccaag gtccataatc tcttatccaa taggtatcat tatccgtaac aatataacgc
     1261 acattagcca tattaacacc attggaaaca agagctgaat tacaggttgc ctgaagtgaa
     1321 ctggatacga tcacatacaa aagacaatta ttagataaat cagctatcag agaatagggc
     1381 aaaccaaaag gataacgaat tattgaacct tgagcaggtt cccattctgc tacaaaacga
     1441 ggttggtctg gaggaacatt aacctgttgt aaggaaccag tgtaagttac tctattaaaa
     1501 gcagtaaccg ttactgtata tgtaatttct cctgatggag gattgctgat attaacaatt
     1561 gccgaaccac tactattggt aaccgctttg ccgtaaatag tattattata ggttattgcc
     1621 acaagagcat ttgctacccc tgaagagata ataatattat ttgttcctga agttacggat
     1681 gcagggtggc taactgtcat tgccagaggg gtctttgaac ggacctgaag ggatgcatct
     1741 ccgaaaatat gccaggtttt aaacatatct acaccatcat acccataaac atccatcatt
     1801 ttgcaggaac cgttataata caaaccaccc agggttgtat gcacttctcc agttaagagg
     1861 tctgtaactt catcttgagc tcgcatagga gaagcccagg attgatttat agaactggca
     1921 taaattccaa ctgctccccc attggcattt ctcatccagg cttcagcaaa acaggtataa
     1981 gaaacaaaat taccgttcac acaggcaaca tccataataa agggaacttt attgccatta
     2041 gtaagagctg aagcattagt attgctaaaa ccagttgttc cccaggtagt atcggaacca
     2101 tgacctacat aattgataaa accccgccca gtgtttacat tagtagtaac cgtggaagca
     2161 gaggcacccg gatcataaat ttgatccaca ctggtgtagc cataatttag caatttagtc
     2221 cgaatcaaat tcatatgggt tatatcactt tctccattat caccttcgcc actagttcct
     2281 tcagcagaag caattcccat tgctttggat agccaagtag ccgaagtatt caagtccttt
     2341 tcgtattgga ttgttttatt cacttgcgct gttacttctg ctgtagtttg agctgaaaac
     2401 cttccaataa aaatatcggg ataattatca ctacctgcta caagagaaaa accgggatca
     2461 cttcctcctc cgctataaga aagagttgga atttgaggtg catcaccaac cagttgcaca
     2521 aaggtaatag aattatctgc attatagcga ttttgaatgt aggccttaag ttgattggca
     2581 gtagttccga ttgtgctgaa ttccaccaac tcggtatcta tccctttctg ccttttccaa
     2641 ttaatatagg gagctatctg agttaaaaaa cttgaatgac agataaccag taatttaccg
     2701 taggaatcat ctacaggagt ataacgatag gaattccagt tcaaaaaatg gttttcataa
     2761 agaggggaaa aactacggga aacagaagtc cggaaacggt tgaaagtgtt tacggtatca
     2821 tttccttggt tataaacttt cactttataa gaagtataaa ctcttaaagt tcccgtaact
     2881 ggattatagg caaaaggagt agttaaaacg gttatcccac ggaaatccct taaaatatag
     2941 ggctctgata aagcaaccat tttttgagga tagaaagaat tttcttgata gacattgtca
     3001 aaagtgtaag gaattgtagc cggatttata tcacgcgtaa taactccttt ggaaggagct
     3061 accttgatgg gatagtcctt aaattcaaga tcaaaaacct caatcgccat caatgcttga
     3121 tccggaataa taatactgcg gttaaaaaca ggcaattcgg gatagccctt atcttgagtt
     3181 attccttctt taggcagacg aatttgatac cattcctcac cttctatagt gatcatattt
     3241 ttttcaaatt cacctattcg gtattccagg atggtttcgc tttcattatc aataagcagg
     3301 gatacggaat ttttactatt accctcagca atcaattctg cacttaagct aaaagacagg
     3361 agcagaaata tagctggtaa tattgccatt tttttcataa aaggtccttt actcatccgt
     3421 aaatactatt ttccttctca ggaatgttaa actttgatga gctgttataa ttttatttaa
     3481 tcttcttttt tggtttcctc ttgcgttgct aaaggttcag atttcttttc ttctgtttcg
     3541 tctgtaacag gtatcactat tgtctcggat tcttctcttt ctttttcagt tttttccgat
     3601 tctctttcct cttcagaagt ttcttttata atagttattt cttccggttc tatatgttcg
     3661 ggttcaggaa ttacaggttc cacaggaatt tcttctgttt ctatcttttc ttcagcttca
     3721 atttccacag gaatttcttc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nncagtttca atttccacgg gtatttcttc tgttgttatt ggttccggtt cctctattac
     3901 aggtatttct ataggttccg gttcctctat tacaggtatt tctataggtt ctggttcttc
     3961 cataacaggc atttctacag gttctggttc ttccgtaatt gggatttcta tggtttcagt
     4021 ttcttttatt tcaggaataa tttcctcttc cttttccgtt tcttttgttt cttcctgttc
     4081 ttctattttg ctctgaattt cttctgttat tgtctgttcc ggttcagtta ttttttcctc
     4141 ttttaaccct atagtttctt gtatttcttc ttttattagt tccgtctttg ggggagtaat
     4201 ttctttttct tcgggtttgg ttgtttcttc ctcttccttt ttctgaacga atttcttatc
     4261 gcgagtttct ttttcctgtt ttttcttttg ttgacgagca actttttccc gcatatactg
     4321 ttcatgaaga gctatatatt cttcctgcag tttaggatcg cgagagaaga agaatgcctt
     4381 aacagagaga attaaacgcc gattttccat atccagttca ataaccttta agggaatttc
     4441 ttctccgata taaaaagcat cttcgctatg ttccagcttg ggaattgcca gatgcgaaac
     4501 aggaacaaaa ccttctacaa catcattatt tacaggaata tcaactaata cacctttagg
     4561 aataagtttg cctatttttc cctttacttc agtattgata ggtaagcttt gatctaatgt
     4621 ttcccaggga tcaggaacca gttgttttac acctaatgca acacgatgtt gagctctatc
     4681 tatagaaaga actactgctt caacttcctg atttttctta tatacttcgc gaggatggta
     4741 aattctttta gtccagctga tatcagaaat atgaattaaa ccgtcaatac cttcttcaat
     4801 ttccacaaaa gcaccaaagg cagttaaact cttaatttta cgagttagaa ttgttccaat
     4861 aggatagcgg tcttcaatag taagccaggg gttgggttcc atttgtttca tacctaagga
     4921 aattcgtttg ttttctttat caatatccag cactatagca ttgattgttt cgccaatttt
     4981 taagaattta tgagcatcgc taatcttttt tgtccagctc atttctgaaa tatgtaccag
     5041 accttcaacg cctggttcaa gttctacaaa agcaccaaag ggttttaagt tcacaacctt
     5101 gccggttact ctactacctt cgggatattt aatttctatg ttttcccagg gatggggaac
     5161 cagttgtttc atccctaagg aaattctatt tgtttcagga tcaaaattta tcactttcac
     5221 ttttacttta tcgccaattt ggagcatttc tgatggatga ttaagtttac cccagctcat
     5281 atctgtaaga tgcaataaac cgtcaattcc gcctaaatca ataaaggcac cgtattgagt
     5341 tatatttttc acttctccat ccagttccgc atctatctga atttttctga gcaattcctg
     5401 tcttttttgt tctgcttctt cttctaaaac ctgacgccgg gaaagaatta tattgtggtg
     5461 ttcctcatca atattcacca ctttgaaata tagctcttta ccgatgaact gatcaagatt
     5521 gggaatcggt tttaaggaca tctgcgaacc aggcaaaaat gcctctattc ctaaaacatc
     5581 aacaatcatt ccacctttaa cgcgccgacg gattattccg cttaatgaac ttttatcggc
     5641 aaatgcctgt ttgatgcgtt caatattgat catataatcg gctttctttt tagaaagtag
     5701 caacttacct tctccgttct caatttcatt aatgtacact ttaattacgg aattgagttc
     5761 cggaattccg gtataggcaa attcggaaat cggtattata ccttcggact taaaacctat
     5821 atccacacgg acatctttat cggtaatatc cacaaccgtg ccttctataa tttcaccttt
     5881 cttgaaactg gagaaagatt cttcatacat agaaagcatt tcttgatgct ctttttcttc
     5941 ctgcgtaagc tccttttttt cctgttcagg ttcaggttca ggagcaggtt cttcaggttt
     6001 tgcctcttcc gaaacagctg tttcggcatt ttcacttaac atttctttgg gaatggcaat
     6061 tgagggttct tcttctttgg gttcttcttc tttgatttct tcctcaactg taaattttga
     6121 catttcgtct tccccgacac ttaaaacggt ttctacttca ggattggtat tttccgtttc
     6181 tttgctttcg ggtgggactg tgttttgatc atgtattaac atgattcctc cttaaacata
     6241 gggatttctt caatacttgt tacggtctta aaatccccgc ttttttgtaa taatacatta
     6301 taaactttta taatcatctc ttccggagtg gaagctccgg cagtgatacc aatagattta
     6361 tatggcgaaa tatcaataga tgctaactgt ttttcatctt ctatatgata agtcggacaa
     6421 taaactgaac tcaacttcgc taaagcacat gtattggaac ttttcagacc gccgattacg
     6481 aacattgcat cactttcttg ggcaagttcc tctgccgctt tttgcctttg tgttgttgct
     6541 aaacagatag tattaaacac tctcagttca ttaaagtttg agagcaattt atataccaat
     6601 tcctgaagct tttctatcct ttgggtagtt tgtgccatta tagccaaacg cttttcttta
     6661 attttcggta tgggatcgtc ttctccgatt acaaatgtct tttcatttcc ataagaacgc
     6721 attcctatca cttcctgatg ttgtttatca cccagaatta atacgggata atcttcctta
     6781 acaagatgct tcaggatagt gtgtgttctt tttacataag gacaagtagc atctatgatt
     6841 tcgttattat ttttttttag tattttatat tcttctttcg tgattccatg tgaacgaata
     6901 ataaccgtgc tgttttttac ctcttccgct ttattggcaa tatgaattcc ctgctcttca
     6961 agttcttgaa caatagcagg attatgaata agctctccta aagtataaac aggtttttct
     7021 gttaattttg catctttcgc catttgaatt gctcgtttta caccaaaaca ataacccaaa
     7081 taatttgcta tacgtatctt gatttttctt tcctcaggtt ctttatccga attgttgtta
     7141 tctatttttt ctattattct gcaggttatt tcgtaaagct tatgcacttg ttcttcaata
     7201 gttaagttgc ttgtatctat taaaatagca tcttctgcag gttttaaggg tgcaagagac
     7261 ctgttggcat ctgcttcatc acgttctttt aattccttta ggacatctgt ataattggtt
     7321 tttatccctt tttctgccag ctctttaaat ctgcgttttg ccctcacttc caaatcagct
     7381 accataaaaa attttgcttc agcttcggga aatacataag ttccaatatc tctgccttcc
     7441 aaaacaaaac ctccattttt cccgagtttg cgttgcaatt caaccatttt atagcggact
     7501 gcaggaattg cagaaatagc cgaagcggaa cttgatattt ccggagtgcg aatttgttgg
     7561 cttacatctt ccccatttaa ccaaataata ttttcctctt cagtatagga tatttggata
     7621 tctatttttt ccagcatacg cactatggat gaaatatcat tgggagaaat tccttcccgt
     7681 attgcctgta atgcacaagc acgatacata gctcctgtat caagatacac atatcccaat
     7741 tttttagcca attctttggc tgttgtgctt ttcccggaag cagcaggacc gtcaatggca
     7801 acaataatct ttcgctttgg caatactttt ctcctttagc gccttttttt tatttcctac
     7861 aataccgtca accctttttt cacagtataa tatctgactt ttattcctta tttatttata
     7921 tacaaacaat taactcctat aaaacataaa aatgattgac actatgccac cattaaaaag
     7981 tttgggtttg aatttaaatt atctatttaa tttctatctt aacactgtat agatcaaggg
     8041 gttacattat gccttacaaa aacaatatga aaattagaca ttattgtgtc aagtgcttga
     8101 ttgctatcgc aatttttgcc atagctgcaa ctaatttatt ctgttacaat acaggagatt
     8161 atagaacaaa atggggcgga aattttgaaa cccttgaatt atgggaatgc tataatggta
     8221 ttggttggat tgatgcaaca caacttccct cttccccttt tgtaaataca atatatatat
     8281 caaaccagac agttactatg aacagttcta tgattattga agggggtctt gttatagtag
     8341 gaacattgca attagcctcg ggagctatat taaccattaa tccttcagta aattgcgaaa
     8401 ttggagtaat tgaaacatat agcggttcaa cattaattaa caatggattt ataaaagcaa
     8461 atagcagttc ttcttcccta aaagtacatg gaggaatact ggaaaacaac ggtattatca
     8521 cctcctcagc tcctaataat tgtaatgtat atataaactc taatggaaga attaactttg
     8581 gaaatcgggg ttcaataacc ggcaattgtt cttttactac taattacgga agcatcattg
     8641 ctacagccaa tactcaaggt ttagacggct cactaaattg cagcggtgat atcagcttta
     8701 accaaattta tcttatttat aatggaactg aacctcaaat aactggaatg aagacaccgg
     8761 atctggtttt gggtatagat tttaataatc ctgcaggcat aaccttaagc aaaaatgtaa
     8821 aacttatata taccgcattg gttcatagcg gaactacact atattttgat gtacacatta
     8881 tcaaggaagc atggtatgga tcaggaactt tttctatgga agatggttct actattgcaa
     8941 ccgctaatcc agatggtttt tggtctactg acaaaaaagg cagtgtgcag gtaggaacaa
     9001 gaaattataa cagcaatggt aactacattt ttaatggcac cgaacatcag caaaccggag
     9061 attttaacac aacgccagat gcttatactg taaatgacat tatttttgat aatcccacag
     9121 gtgtaacttt aactcaccct ataacagttg tatctactct ggaactcctt gaaggagata
     9181 ttaattacac ggttttacct caaggagtag atggttttta tagtcccgat gtaaaaaaaa
     9241 ccgttattct caaaaatggt actctgatgt ataatttttt ggcagattct cttccttttc
     9301 agaataatgg tgagtatgtt aaacgcaaat ggtatctaaa agggaatttc aatggttcta
     9361 aaaaagtaac cttttactgg agtgaaaatg aggatgataa ctacaattgg aatgttcata
     9421 atttcccaaa agtatattta tcaaacagta atgaaccatt gcacactatt tggaatcctg
     9481 cccatcccag agagatttct tttattgcac atagtttccc caatgccaaa gaagatgtat
     9541 attactatat cggtaaagaa agagatgata ccttgcctgt aactttaagt tctttttcct
     9601 taacacaaag tggtatttcc actgttagaa taacttgggt aactcaatcc gaaacaaata
     9661 ttatgggata ttatatttac cgtgctactc aatcagattt gaacacagcc agtaatctca
     9721 attaccttat tccggcaacg aattcttcac aattacaaat atacacctat acagaccagg
     9781 ttatttccaa taatatctac tattactggc tggaaattgt agattacgat ggatataatt
     9841 cgttttatgg acctgctgtt atagaaataa atgataacgg aaatcaaaca ccttccatac
     9901 ctgttaaagc aggattttat tcatttgctc ccaatccttt tgattctggc actaaaatct
     9961 tctatggaat tccctcaaaa tctcccgttt taatgcaagt ttacaatata aaagggcaaa
    10021 aaataagaac gcttttaaat gaaaccaaag atagcggtaa ctataacctt ttctgggatg
    10081 gtagagatga aaaaggaaat ttgttagcta acggaaatta tattctgcgt ctttttgcct
    10141 caggaagttc ttggactaaa aaaattacta tcttgcatta ggacaatctc ttaccttagc
    10201 ataactattg ccttccctaa tagcattact cactcattgc ttacccattg ctaatggcat
    10261 cagtattggg taagcaatgc tttagtattt cgatcaggat ataactttcc cgctttataa
    10321 gtgcttttag gacttgcaaa ttaggtgttt attgattatc agaccattga tctttaacca
    10381 ctaatattgt tgatagcaaa gtaaggtgta aaattgttaa tcctttttct gccaacgcat
    10441 ttcggcatac ttggaagtaa agccgaatct ttcataaaga tgccttgccg gattgtcata
    10501 ttctatatga agtgcgatat ctccatcagc aatttccctt gcctttttta atatactact
    10561 ccctattccc atattgcgca ttgatttctc tacagcaata tataccagaa tattttcggg
    10621 aatatattct tccatccctg ttttattcat tactaaaact ccgcagagtt tatcttctgc
    10681 aaaagccaaa atgaggaatc cacccttccc tttttccgct gaaaaggcat aatcaatggc
    10741 tttgttaatg gctgaaggtg catccccgaa actttccaaa tgcgtatata gaaaatctac
    10801 caaatccttt tgcgaaatgt tttctggtaa ctcatccggt ttgtatataa cctgtaaatt
    10861 aagcataact acattccttt ttactttaac acgaataatg tagttgaaga acaatggatt
    10921 gacaaattct ttctttgata aagaaactga atgttgctaa atgaatttat acttttaagc
    10981 atttgcaaat cagaattccg caatccaatt aattaagata tttatcaact attttttact
    11041 gtatagttca ttttaaattc tatctgcttt ataccataaa gaaattaggc gacttaaatg
    11101 cataatgaaa tctctatttt tttcagctat tttaatccct tgttaaaatc cattctcctc
    11161 ctctgggaaa accgctttgt ttgattaagc catcttttta aagcttacaa attaaccttt
    11221 taattgttgc aatgcttttg ccggtaattc ttgccagttc actatagctt attttagggt
    11281 tatgcgttac taaaaatatc atctgataat catcagttgc ctcaaaaaac ggttccgtta
    11341 aactgcttca ttatcagtta tgcacataga gtttagttag cattttattc tccttttgca
    11401 tacaggttat aaacccttga attattgcaa atcttatgaa atactaaatt atgaaatgat
    11461 gtaaaaatgt caaagcaatt ctcagcttgc ttgtttctct gctgtagcag tgaatttgct
    11521 ttttgggata aaaggattat attattttta gcgaatttat ttatctattt gggcaaaatg
    11581 ccttttttct tgacagttaa accgagcttg tttttttggc aaaagagtat gccgaagtgg
    11641 tgaaactggt agacgcagtg gactcaaaat ccaccgggca atagctcatg ccggttcgat
    11701 tccggccttc ggcaccaaag ttttttagga gataaaaaac atttacttgc tatagggata
    11761 agtcctaaac taatgtagtt gactgtagaa gaggcataat ctaaactgat atttatttct
    11821 cgcagattag attatataaa atcaaatcac acacttcggg tgatatttct ttaaacctga
    11881 aactttttat acaaaacctg tggaggtata atgtttaagc gccacgctat agtattcagc
    11941 gtattgctga tgcttattct accttttgcc ttgtctggaa tttctcaggc aacaatgtta
    12001 ttcttaacct ttga
//
LOCUS       DAOPFL010000050        11814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_6487_length_11814_cov_102.522919, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000050 DAOPFL010000000
VERSION     DAOPFL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6487_length_11814_cov_102.522919"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1607
                     /locus_tag="PLG69_04870"
     CDS             336..1607
                     /locus_tag="PLG69_04870"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPI42876.1"
                     /translation="MASILVLGSMWGDEGKAKIVDYLASSADYVVRFQGGSNAGHTIV
                     SGGKKYVLHTIPSGILYPQTKCLIGAGVLIDPPAVIEEIKTLEQAGINFKNRLLIDLR
                     AGIVLPLHKQIDLEIETKRRGMQIGTTLKGIGPAYSDLTARYGIRMEDLANPITLKYR
                     IKQLYGYHHIGIGKQDLDILVEKLHKDWKYLKKYVGDVEILLNNIAVKEEKTILFEGA
                     QGTLLDLTFGTYPYVTSSRVMTDGVGIGTGFSARRLNKVLGVYKAYATRVGIGPFPTE
                     MIYEEEVDYIRKIGNEYGSTTGRPRRIGWFDAVAGKFSARINALDNAVLTCLDVLTGI
                     EELKICTGYKYKGKIHCSLDLNELQLILTEPVYETFDGWDTDISNCKNINKLPLTARL
                     YIKAIEDHIQTPISLVSVGKERHQTIEIKPL"
     gene            1620..2792
                     /locus_tag="PLG69_04875"
     CDS             1620..2792
                     /locus_tag="PLG69_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42877.1"
                     /translation="MIKRLFFTVIFCLLAIFLLAQADSLYLLLPQFSNPFYDTFSRNY
                     IGSTSAGRGYTGVSVLGGLDNALLNPASVLPDSAKFFIEMNIKPSLEAEGYPLYANYT
                     SPFPLGLLGLSFALENKFSLGVLYNLPKSITLDDYSFFINQGADLIQRFPVYNLHQAT
                     ALIAFHNGPLHLGLNLHNQFHYIDDPIFLRSYERIRDSQYHLRLQPGIIYQLGSANIG
                     FSMMPATKFSWNLKCANYDFKMPLWLTGGIEFVKPEFTISTEAEWEQTSDICDSFNDR
                     FTFKAGLEKMSDKLVYRLGYHFTSNVYSGMIRLAQNVVNPDTSYVWNTVPQAVFIEDN
                     AQHSLTAGLSYRFKNGIFNLSAMQVILGDVNKTQINVSLCFYLNYIFRKREIHLNE"
     gene            2785..3909
                     /locus_tag="PLG69_04880"
     CDS             2785..3909
                     /locus_tag="PLG69_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPI42878.1"
                     /translation="MNKPAVEIRRIDSYDLPVIEEAVADFFKEIKSPKINHSKRVLIK
                     PNALGAYPPERAVTTHPIVLEAIIRYFLDRKKEIWFGDSPGNSVAFETVWQTCGFSSL
                     AEKYHLKVVNFSTLGFRELSYKDISIKVSEALFQCGIVINVGKYKTHQLTAFTGALKN
                     LFGFVPGLAKSEYHRLYPDTNSFAAMLTALYAVIGNRITYSIIDGITGMDGNGPSAGK
                     PHHFGLLFGSTSIPALDYVASRIMGFQLKDVPYLSSALIMDGILPSQIKIPVSFRNYR
                     IPDADINSVKVIQKTLKYVPGIARKAFRKVYYFYPAVSERCKQCGICVKSCPVKAISW
                     QNNTKPYIHKEQCIKCLCCHELCPYQAIDIKKSLLARLGV"
     gene            3909..4232
                     /locus_tag="PLG69_04885"
     CDS             3909..4232
                     /locus_tag="PLG69_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPI42879.1"
                     /translation="MKSKTKKLSPTLRFAFWAIIVLLIIWILFLANNSFLNTWKLKRR
                     VEQLEKETSILKAQNDSLAQENERLKTDPEAAEKAARENFGLTKPDETVFRFVPAKED
                     EPPKK"
     gene            4213..5913
                     /locus_tag="PLG69_04890"
     CDS             4213..5913
                     /locus_tag="PLG69_04890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPI42880.1"
                     /translation="MNPQKNSQPHIYNISLFTNVEQDTRNKLKKLVHDLILANEDSRL
                     LIIEKIADIVIPACSGNLDNQLFSEFSGLLIEYLLDYFLELKSTQESEFYHFLSFLSH
                     CPVTEDSPYYPYVQKIMNDHKESEPELMNIFNRLQLLYLLTENGDFGNAEELLEELEP
                     VEENKHLELWALFQLSQTRIYRHQNKPAELLETQLNLILEAYIRDSSDSAINFIIRWL
                     ISINWFKQNIIKKTLLLRIKDYLLEQKSLNTAMVLYELFSMEDRLVPSVEKIEYQRIL
                     IKYPVSMLNVQQLHTLYFFAGYYNCGVLSNFKDSIQNYQYSNYFLHKSWDSFIHLSRF
                     MREHLDPSRFYNAVPYLESRIKELSNQASLQNNAYVESLQANFYKIEELYEKVGELSL
                     TDSLTGLRNRRYLEGNLFQMVILAARHNVPVCFSMIDIDFFKLVNDNYGHLAGDFVLK
                     ELARILNSEFRKSDVVIRYGGDEFLVILFDSDQKLSYSIMEDLRQKIEAYSFDYNNQI
                     ITFTISIGIACDFHISSQPKNLAKCIACADQACYVAKTNGRNRVELYQSPTGATDNNC
                     "
     gene            5986..7089
                     /gene="ugpC"
                     /locus_tag="PLG69_04895"
     CDS             5986..7089
                     /gene="ugpC"
                     /locus_tag="PLG69_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPI42881.1"
                     /translation="MAKVTVKNLNKYFDNGVHAVKDVTFTAEDKEFVVLVGPSGCGKT
                     TILRMIAGLEEVSSGEIYIGDVLVNNVPPKERDIAMVFQNYALYPHMTVFDNMAFALK
                     MRHIPKEDIKRIVYDAAKLLGIESLLKRKPGQLSGGQRQRVAVGRAIVRNPKVFLFDE
                     PLSNLDAKLRVAMRAEIVKLHHTIGNTMIYVTHDQVEAMTMADRIVVMKDGVIQQIDT
                     PLNIYNNPANMFVAGFIGSPAINMTKGKLIRKESDLYFDSEDYQLKLTPRQAEVLQNY
                     ADKDIIMGIRPEDIYDSRFDAMAESPQKFITKVDFVEPLGNEYHVILTTANNEYTARF
                     DPKDLPKMGQDLSVTFDMAKAHFFDPESEVNLY"
     gene            7125..7904
                     /locus_tag="PLG69_04900"
     CDS             7125..7904
                     /locus_tag="PLG69_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42882.1"
                     /translation="MWFKTGKQYIYLLSVTFILFCSNPFLFSVTTENSASANALSGIT
                     MLSASPADYTLSPVIGESGICFSWHQPFSISDINVYGLHSAFPVKPLIVATGIDYLAH
                     TDYRWQNEYLALSASFSFFRIGATQHLIYEKIAEQSWYTWDNDFALSFQNKRFGSEIR
                     GNNIRTEERTFTLSANFNANENSSFATSYSFRPKEKDSYSLATSLAIAKPLIIQSSWQ
                     SEPARFGLGLKVLIGKVNLMYAVRTHTELDLTHIVDLGSAW"
     gene            7901..9409
                     /locus_tag="PLG69_04905"
     CDS             7901..9409
                     /locus_tag="PLG69_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42883.1"
                     /translation="MKVFGCFLVLFTFCCFPLFSQIEEIPELESETLEDNWLNQLYTE
                     LETGKHKVDLQSRTKYQETFANGLANLQFRQKDMRFNLNLYKKENSDLYANFQLSAMP
                     SKNKSSEIHFGSFRPAFGMGTILKKSGGNSLFAINRAGHPSSFSPFGTGAILRVNNFS
                     AFFMASGQKRNASLKEGKIGSLYKSVQSEKTSVQEEIIAGGLEYHYKNNNFALLAYNQ
                     NYDRDFADTLWVKKLNAYSISAKTTGENYVLSAETALLEENIAFQSSVKFIYNYISQE
                     LSYSYRQGKQLPAYAAKPYLLSFQGENREIDWNLDYQLPKNMSLGLRYALMRKNNALK
                     SPNWNSRGIFNLTFNPSYTIINLQLTLLDKEIVTETDSSYIATLPVHYRLRFKIDHEL
                     NPKLTLSLLLRYHYEDKIERRNNSFYWENAFYYHRTKWQISAGIKTWQTLTSLILPET
                     DTENPEGCICATSEDDRVFAKVTYKGKVINLKTEWQQSWLNGNRTLYLSVGL"
     gene            complement(9823..10476)
                     /locus_tag="PLG69_04910"
     CDS             complement(9823..10476)
                     /locus_tag="PLG69_04910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPI42884.1"
                     /translation="MQENKTASFFDRYSIDFNAIYGSGTGPFQRLINKWFRQTMLIRF
                     QKTIVACNPLEGKTILDVGCGPGIYSITLAKGSPNFVYGIDFAPSMIEIARQEAKKAG
                     VENICRFEIADFNTLPEDKQYNYLILMGFMDYMKNPKQVIEKTMRLASDKVLFSFPSD
                     KGFLAWQRKIRYKFKCPLYLYNEKQLKNLFSDISPWHYTLENIGRDFFVTLEKGENL"
     gene            complement(10531..11403)
                     /locus_tag="PLG69_04915"
     CDS             complement(10531..11403)
                     /locus_tag="PLG69_04915"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPI42885.1"
                     /translation="MREYPKSIFTIDVEDYFNVTEERGEPPVSEWDSLPSIVESGFLK
                     LLDLLDTYQVKATCFFLGYVAKKYPHLVKEAQKRGHEIASHGMFHRIVYKMSKDEFTA
                     DLLQSKNILEDICSEQVTAFRSPSFSLTEASPWFFETLAETGFIADSSVFPVKRDYGG
                     YKTTQKKPYWITTEKGDICEFPISVAPFCGKDICFFGGGYLRLFPKHVILYMHKKLAK
                     QGIPTLFYIHPREMEPQHPRLKMNKMAYFKSYVNLNTVQPKLEAILSSSKFITCRQYI
                     ETCFPGKRMSSSFT"
BASE COUNT         3635 a         2198 c         2266 g         3715 t
ORIGIN      
        1 atacaacagt gtgttataac ttgtccacat cacttttttg tgggaatgcc ttgaaatgaa
       61 gggtgaaaaa tgaaagatga aggttgaaaa taatggtcct tccggtattc tgaaccacaa
      121 ctgacttgcc tgagcactat ttcctttcgt tatttctgag ccatcacgat cttatgcttg
      181 ggtttcactt tttcactttt tcactttttc accattcgcc cgttaaactg ttaacccgtt
      241 cacccgttca cccgttcaat cgctaacctg aagaaataga ttgacagaaa tagaaataat
      301 gattaagatg ttttatataa tataaggaga agataatggc atcaatattg gttttgggaa
      361 gtatgtgggg tgatgaagga aaagctaaaa tagttgatta cttagcttct tcagcagatt
      421 atgttgtgcg ttttcaaggt ggaagtaatg ccggtcatac tattgtaagt ggtggaaaaa
      481 aatatgtgtt acatactatt ccgtcaggaa tcttatatcc ccaaaccaaa tgccttattg
      541 gagcaggtgt tttaattgat ccaccggcag ttattgagga aataaaaaca ctggaacaag
      601 cgggaattaa ctttaaaaat cgtcttctta ttgaccttcg ggctggaatc gttttgccac
      661 ttcataaaca aatagacctg gaaattgaaa ctaaacgcag aggaatgcaa attggcacaa
      721 cccttaaagg aattggtcct gcctacagtg atcttactgc cagatatgga attagaatgg
      781 aagacctggc aaaccccatt actttaaaat atcgtattaa gcaattatat ggttaccatc
      841 atattggaat aggtaaacag gacttggata tcctggtgga aaaactgcat aaagattgga
      901 aatacttgaa gaaatatgtt ggcgatgtgg aaatcttgct taataatatt gccgttaaag
      961 aggaaaaaac aattctcttt gaaggagctc agggaacttt actggattta acttttggca
     1021 cttatcctta tgtaacttcc tccagagtaa tgactgatgg cgttggaatt ggaacaggat
     1081 tttcagccag gcgtttgaat aaggttttag gtgtttataa ggcatacgct accagagtag
     1141 gaataggtcc ttttcccaca gaaatgattt atgaagaaga agttgattat attcggaaga
     1201 taggaaatga atatggttct acaacaggaa gaccccgaag aattggctgg tttgatgctg
     1261 tagcaggaaa attttctgcg cggataaatg ctttggataa tgcagttctc acttgcctgg
     1321 atgttctaac cggcatagaa gaattaaaaa tctgcaccgg ttacaaatac aaagggaaaa
     1381 ttcactgttc tctggatttg aatgaattac aattaattct taccgaacct gtatatgaga
     1441 catttgacgg ctgggataca gacatcagca actgcaaaaa tatcaataag ttgcctttga
     1501 cagcccgact ttacattaaa gccatagaag atcatattca aactcctatt tcgcttgtat
     1561 cggtaggtaa agaaagacat caaacaattg aaattaaacc cctgtagagg taaggaaaga
     1621 tgattaaacg cttgtttttt actgttattt tttgtctgct ggctattttt ttgctggctc
     1681 aagctgattc cttatatctt ttgcttcctc aattcagcaa tcctttttat gatactttca
     1741 gtcggaatta tatcggttca acttctgcag gaagaggtta tacgggtgtt tcagttttag
     1801 gtggtttaga taatgctctt ttaaatcctg cttccgtttt acccgattca gctaagtttt
     1861 tcattgaaat gaatataaaa ccatccttgg aagcagaggg ttatcccctt tatgctaatt
     1921 acacttctcc ttttcctttg gggcttttgg gtttaagttt tgccttggag aacaaatttt
     1981 cccttggtgt gctttacaat ttgcctaaat ctattaccct tgatgattat tcctttttta
     2041 tcaatcaggg agcagattta attcagcggt ttcctgttta taatcttcat caggcaactg
     2101 ctcttattgc ttttcataac ggacctctgc atttgggttt gaatttgcac aatcaattcc
     2161 attatataga tgaccccata tttttaagaa gttatgaacg cataagggat tctcaatatc
     2221 atttgcgcct tcagcccgga attatatatc agcttggttc tgctaatatt ggcttcagta
     2281 tgatgccggc taccaaattt agctggaatt tgaaatgcgc caactacgat ttcaagatgc
     2341 ctctttggct tacaggagga attgaatttg taaaaccgga atttaccatt tccacagaag
     2401 ccgaatggga acaaacaagc gatatttgtg atagttttaa tgatcgtttt actttcaaag
     2461 ctggtttgga aaaaatgagc gataaattag tttaccgtct tggctatcat tttacttcca
     2521 atgtttacag cggtatgatt cgtttggctc aaaatgttgt taatcctgat accagttatg
     2581 tctggaatac agttccgcag gcagttttca ttgaagataa tgcacaacat tctttaactg
     2641 cgggcttaag ttatcgtttt aaaaatggta tatttaatct ctctgcgatg caagttattt
     2701 tgggagatgt gaataaaacc cagataaatg tctccctttg tttctattta aattatatat
     2761 tccggaagag ggaaattcat ctaaatgaat aaaccggcag tagaaattcg cagaattgac
     2821 tcctacgatt tacctgttat agaagaggca gtagccgatt tcttcaagga aataaaaagc
     2881 cctaaaataa atcatagtaa aagggtatta attaaaccca atgccttggg agcttatcct
     2941 cccgaaagag ccgtaactac acatcctata gtgctggaag caattattcg ttattttctg
     3001 gacaggaaaa aagagatttg gtttggtgat agtccaggta attctgttgc ttttgaaact
     3061 gtctggcaaa cctgcggatt ttcttctctg gcagaaaaat atcatcttaa agtagtcaat
     3121 ttttctactt tgggctttag agaattaagc tataaggata ttagcataaa ggttagcgaa
     3181 gctctgttcc aatgtggaat cgttatcaat gtaggcaaat ataagaccca tcaattaacc
     3241 gcattcacag gagctctgaa aaatcttttc ggctttgttc caggattggc gaaaagtgaa
     3301 tatcatcgtt tatatcccga tactaatagt tttgctgcta tgcttaccgc tttgtatgct
     3361 gtaattggca accggataac ctatagcata attgacggta ttacaggtat ggatggcaac
     3421 ggaccttctg cagggaagcc acatcatttt ggacttcttt tcggttcaac ttccattcct
     3481 gcattggatt atgtagcatc acgcataatg ggttttcagc taaaagatgt tccctattta
     3541 agttccgctt tgattatgga tggcatttta ccttcacaga ttaaaattcc tgtttctttc
     3601 cgcaactaca gaatccctga tgcggatata aatagcgtaa aagtaatcca gaaaacgctg
     3661 aaatatgttc ccggtattgc tcgtaaagca tttcggaaag tgtattactt ttatcctgcg
     3721 gtaagtgaac gctgtaaaca atgtggaata tgcgtaaaga gctgtccggt aaaagctatt
     3781 tcctggcaaa ataataccaa gccctatatt cataaagagc agtgtattaa atgtctctgt
     3841 tgtcatgaac tctgtcccta tcaggcaata gacattaaaa aatcattact ggcaaggtta
     3901 ggtgtttaat gaaatccaaa acgaaaaagc ttagtcctac tttgcgtttt gccttttggg
     3961 caataatagt cctgttgata atctggattc tatttttagc taataacagc tttctgaata
     4021 cctggaaatt aaaacgccgg gtagaacagc tggaaaaaga aacctccatc ctcaaagctc
     4081 aaaatgatag tttagcacaa gaaaatgaac gcttaaagac cgaccccgaa gccgctgaaa
     4141 aagctgcacg ggagaatttt gggctgacta aacctgatga aaccgttttt cgtttcgtcc
     4201 cggcaaaaga agatgaaccc ccaaaaaaat agccaacctc atatttacaa tatttccctg
     4261 ttcacaaatg tggaacagga tactcgtaat aagctgaaga aattagttca cgatttgatt
     4321 ttggcaaatg aggacagccg tctgctaatt atagaaaaga ttgcggatat agtgattcct
     4381 gcctgttcag ggaacctgga taatcagctt ttcagtgaat tttccggttt gcttattgaa
     4441 tatctgctgg attatttcct ggagctgaaa tcaacacagg aaagtgagtt ttatcatttt
     4501 ctcagttttc ttagtcattg tcccgtaaca gaggattcgc cttattatcc ttatgtgcaa
     4561 aagattatga atgaccataa ggaaagcgaa ccggaattaa tgaatatctt taatcgcctt
     4621 cagttgcttt atcttttaac ggaaaacggg gattttggga atgcggaaga actgctggaa
     4681 gaactggaac ctgttgaaga aaataaacat cttgagcttt gggctttatt ccagctttcc
     4741 caaacccgaa tctatcgtca tcaaaataaa cctgccgagc ttttggagac gcagttaaac
     4801 ctgattttag aagcatatat aagggatagc agtgatagcg ccatcaattt tattatccgt
     4861 tggcttattt ctattaactg gtttaagcag aatatcatca agaaaacctt attgctgaga
     4921 attaaggatt accttttaga acagaaatcc ttgaacacgg caatggtatt gtatgaattg
     4981 ttttcaatgg aagaccgtct tgtgccttcc gttgaaaaga tagaatatca acggatactg
     5041 attaaatatc ctgtttcaat gctgaatgtt caacaacttc atacgcttta tttttttgcc
     5101 ggatactata actgtggtgt gctttccaat ttcaaggatt ccattcagaa ttatcaatat
     5161 tccaattact ttttgcataa aagctgggat tcttttatcc atctttcccg ctttatgcga
     5221 gaacaccttg atccttcgcg tttttataat gctgttccct atctggaaag tcgtatcaag
     5281 gaactcagca atcaggcatc tttacagaat aatgcttatg tagagagctt acaagcaaac
     5341 ttctataaaa ttgaagaact ctatgagaaa gtgggtgaac tttctttaac cgatagctta
     5401 accggcttaa gaaatagacg atacttggaa ggaaacctct ttcaaatggt aattttagcg
     5461 gctcggcata atgttcctgt ttgtttcagt atgatagata ttgatttctt caagctggta
     5521 aatgataatt acggtcactt ggcaggggat tttgtgttga aagaactggc tcgtattctc
     5581 aattctgagt tccgtaaaag tgatgttgtc atccgttatg gaggcgatga gtttttagtt
     5641 attctttttg attccgacca gaaattaagc tattccatta tggaagacct ccgccaaaaa
     5701 attgaggcat attcctttga ttacaacaat cagattatta ctttcacaat tagcatcgga
     5761 attgcctgtg attttcatat ttcctctcag cccaaaaacc tcgccaaatg tattgcttgt
     5821 gctgatcaag catgttatgt agcaaaaact aatggacgca accgggttga actctatcaa
     5881 tcaccaactg gagctacgga taataactgt tagaataata tcaataattt ctctcttgac
     5941 atatttccct attacctgct ttaagtaaac acaaaaggag taataatggc taaggttacc
     6001 gtaaaaaacc tgaataaata ttttgataat ggtgttcatg ctgttaaaga tgtaactttt
     6061 actgcggaag ataaggaatt tgtagttctt gtaggtcctt ccggttgtgg aaagaccaca
     6121 attttaagaa tgatagctgg tttggaagag gtctcttctg gggagattta tattggcgat
     6181 gttttggtta ataatgtgcc tcccaaagaa cgagatattg ctatggtttt tcagaattat
     6241 gctctctatc cgcatatgac ggtgtttgat aatatggctt ttgctcttaa aatgaggcat
     6301 attcccaaag aagatataaa gagaattgtt tatgacgcgg caaaattatt gggcatagaa
     6361 tcactcctga aaagaaaacc aggtcagctt tccgggggtc aaagacagcg tgttgccgtt
     6421 ggaagagcca tagttcgcaa tccgaaggtc tttttatttg atgagcccct ttccaatctg
     6481 gatgccaaat tgcgtgtggc aatgcgggca gagattgtta aattgcatca tactattggc
     6541 aatactatga tttatgttac gcacgatcaa gtggaagcaa tgactatggc agaccgcatt
     6601 gttgttatga aagatggcgt tattcagcaa atagataccc ccttaaatat ctataataat
     6661 cctgcaaata tgtttgttgc cggctttatt ggcagtcctg ccatcaatat gacgaaaggc
     6721 aaattgatta gaaaagaaag tgatttgtat tttgatagtg aagattatca gctgaaactt
     6781 acgcctcgcc aggcagaagt tctccagaat tacgcagata aggatattat tatgggcatc
     6841 agacctgaag atatctacga ttcccgtttt gacgctatgg ctgaatcacc ccagaaattt
     6901 attactaaag tggattttgt agaacccttg ggaaatgaat atcatgttat tttaactaca
     6961 gcaaataacg aatatacggc gcgttttgat cctaaggact tacctaaaat gggacaagac
     7021 cttagcgtta cttttgatat ggctaaggca catttctttg atccggagag tgaagtaaac
     7081 ctgtattgag cccataacat cttatcagtt taagagttta tgctatgtgg ttcaaaacgg
     7141 gaaaacaata tatttatctc ctgagcgtaa cttttatcct gttttgttcc aaccctttcc
     7201 tgttttctgt tacaacggaa aattccgctt ctgctaatgc cttatcagga atcactatgt
     7261 tatcggcttc acctgcggat tatactcttt ctcctgtaat tggggaaagt ggaatttgtt
     7321 ttagttggca tcaaccattt agcataagcg acatcaatgt ttatggattg catagtgcct
     7381 ttcccgtaaa accgttgata gtagctacgg gaattgacta tctggcgcat acggattatc
     7441 gttggcaaaa tgaatatttg gctttaagtg caagtttttc cttttttcgc ataggcgcaa
     7501 ctcagcattt aatctacgaa aaaatagcgg agcaaagctg gtatacctgg gataacgatt
     7561 ttgctctttc ctttcagaat aaaaggtttg gaagcgaaat acgcggtaat aatatccgca
     7621 cggaagaacg gacatttacc ctttctgcca attttaacgc caatgaaaat tcctcctttg
     7681 ctacatcata ttccttcaga cccaaggaaa aggatagtta ttctctggca acttctcttg
     7741 ccatagcaaa acccttaata attcaaagtt cctggcaaag tgaacccgcg cgttttggac
     7801 tgggactgaa agtgctgata ggtaaagtga atttgatgta tgctgtgcgc actcatacgg
     7861 aacttgattt aactcatatt gtggatttgg gctctgcctg gtgaaggttt ttggctgttt
     7921 tcttgtcctc tttacttttt gctgttttcc tttatttagc caaatagaag aaataccgga
     7981 gctggaatcc gaaaccttgg aagataactg gctgaatcaa ctttacacgg aactggaaac
     8041 aggaaagcat aaggtagacc ttcagagccg gacaaaatat caggagacct ttgctaatgg
     8101 actggcaaat cttcaattcc gccagaaaga tatgcgcttt aaccttaatc tttataaaaa
     8161 ggaaaattca gacctttatg ccaatttcca gttatcggct atgccttcta aaaacaaatc
     8221 ctcggaaatt cattttggtt cctttcgtcc tgcttttggt atgggaacta ttctgaaaaa
     8281 aagcggagga aattcccttt ttgccataaa ccgagcaggg catccttctt ccttttctcc
     8341 ttttggaaca ggagctattt tgcgggtaaa caatttttcc gctttcttta tggcttccgg
     8401 tcaaaagcgc aatgcttctc tgaaagaagg caaaatcggg tcactttata aatcggttca
     8461 gtccgagaaa acttccgttc aggaagaaat tatcgccgga ggactggagt atcactataa
     8521 aaacaataat tttgccttgt tggcttataa ccagaattac gatcgggact ttgccgatac
     8581 gctttgggta aaaaaactga atgcctactc aatttcggct aaaacaaccg gggaaaacta
     8641 tgttttatct gcagaaacag ctcttctgga agaaaacatt gcttttcaat cctccgttaa
     8701 attcatttat aactatattt ctcaggaact cagttattct tatagacagg gaaaacaatt
     8761 gcctgcttat gctgccaagc cctatctatt aagctttcaa ggagaaaata gggaaattga
     8821 ctggaatttg gattatcagt taccgaaaaa tatgtccctc ggtttgcgct atgctctaat
     8881 gcgtaaaaat aatgccctta aaagtccgaa ctggaattca cgagggatat tcaatcttac
     8941 ttttaatccc tcttatacca ttataaattt acagttaacc ctgctggata aggaaatcgt
     9001 aactgaaacg gattcaagct atatagctac tcttcctgtc cattatcgtt tgcggtttaa
     9061 gatagaccac gaattaaatc cgaaactgac cttatctctt ttgctccgtt atcattatga
     9121 agataaaatt gaacgccgaa acaacagctt ttattgggaa aacgcttttt actatcacag
     9181 gacaaaatgg caaatttctg ccggaattaa aacctggcaa acccttactt ctcttattct
     9241 gcccgaaaca gacacggaaa atccggaggg atgcatttgt gccaccagtg aggatgatag
     9301 ggtctttgcc aaagtaacct ataaaggaaa agtaattaac ctgaaaacgg aatggcaaca
     9361 atcctggcta aacggaaatc gcactctcta cctttcagta ggattgtagc attatcggca
     9421 ataaggatta tatagatgat actgatttat acggattaaa tttccgctga ataaaataga
     9481 gataaaagat tgtgagagtt gaaagcaaag agatcagagc agaaaatgaa ggggtttaaa
     9541 aagcagaaag caaatagcta aaagccaaga gatgaagata ccggaaggta aatttcctcg
     9601 taaatcacta ttagcgcaaa gtttagaggg ttgaaaaggt ttagaaggtt gagcaagaat
     9661 acggctcttg tctattcaat tatatatttt cctgcaatat ttgtaactac ttctaaatat
     9721 agacagttcc aatttcttcc ttaatagcat tgcttaccca ttgcttaccc attactaatg
     9781 ccatccgtaa tgggtaagca atgggtaggc aattctatct gcctaaaggt tttccccttt
     9841 ttccaaggtc acaaagaagt ccctccctat attttcaagg gtataatgcc agggtgaaat
     9901 atccgaaaac aggtttttca gttgtttttc attataaagg taaagtggac acttaaattt
     9961 atagcggatt ttccgttgcc aagccaaaaa acctttatcg gaaggaaaac tgaaaagaac
    10021 tttatcactt gccaaacgca ttgttttttc aataacctgt ttgggatttt tcatataatc
    10081 cataaaaccc attaaaataa ggtaattata ctgcttatcc tccggcaggg tattaaaatc
    10141 ggcaatttca aaccggcaga tattttccac ccctgctttc tttgcctctt gtctggctat
    10201 ttctatcatt gaaggtgcaa aatcaatacc gtaaacaaaa ttcggagaac cttttgctaa
    10261 agttatagaa taaattccag gaccacaacc aacatccaga attgtctttc cttccaaggg
    10321 gttacaagca acaattgtct tttgaaaacg gattaacatt gtctgccgaa accatttatt
    10381 gattaagcgt tgaaaaggtc ctgtgccgct accgtaaata gcattaaaat caattgaata
    10441 gcgatcaaag aaagaggcgg ttttattttc ctgcatttaa ttatccttca tagttttaat
    10501 atagttcctg ctgactacaa atcttcctta ttaagtgaag gatgaggaca tcctttttcc
    10561 cggaaagcag gtttctatat actgccggca ggttataaac ttactgctgg aaaggatagc
    10621 ttccagctta ggttgcaccg tattcaggtt tacataactc ttgaaatatg ccattttgtt
    10681 cattttcaaa cgcggatgtt ggggttccat ttctctggga tgaatgtaaa aaagagtagg
    10741 aattccttgt tttgccagct ttttgtgcat atacaaaata acatgcttag gaaaaagacg
    10801 caaatagcca cccccgaaaa agcaaatatc tttaccgcaa aagggcgcta cagaaatcgg
    10861 aaattcacat atatcacctt tttctgtcgt tatccaatag ggctttttct gcgttgtttt
    10921 gtaaccgcca tagtcccttt ttacgggaaa aacagaagaa tcggcaataa aacctgtctc
    10981 tgccaaggtc tcaaaaaacc agggacttgc ttccgttaaa gaaaaactgg gactcctgaa
    11041 agctgtaacc tgctcggaac aaatatcttc cagaatattt ttggattgta gcaaatcggc
    11101 ggtaaattca tccttgctca ttttataaac aatccgatga aacattccat gcgaggcaat
    11161 ttcgtgtccc cttttctgtg cttctttcac taaatgcgga tattttttgg ctacataacc
    11221 caaaaagaaa caggttgcct tcacctgata ggtatccagc aagtccagca acttcaaaaa
    11281 accgctttct acaatactgg gtaaagaatc ccattcggaa acaggtggtt cacctctttc
    11341 ttcggttaca ttaaaataat cctccacatc aatagtgaag atggactttg gatattctct
    11401 cataaatttt tagctaaatt cctataaaga tataatatca tttctggaat gaaaagtcct
    11461 ttgttattcc tgcgaaaaca agaattcaca gaaggtaaaa aataattatg cattttcaaa
    11521 cagaaatttt aataaattcc ctaattgaag aagagtgaaa aatgtcaact gctttcttgg
    11581 ggaatattat atacaccgaa aacactgaaa atattgttta tttacactat tagaaaccga
    11641 ggaaagagtg aattaaactc tttttctcgg tgttcttagt gtcttcagtg ttctcggtgt
    11701 aaaaaatcgc tacaatttac agcttacgca tatattccag caagtccact actctgttag
    11761 aatatccata ttcgttatca taccagctga agaccttcac cattttacct ttgg
//
LOCUS       DAOPFL010000051        11737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_6562_length_11737_cov_90.085773, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000051 DAOPFL010000000
VERSION     DAOPFL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6562_length_11737_cov_90.085773"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..362)
                     /locus_tag="PLG69_04920"
     CDS             complement(48..362)
                     /locus_tag="PLG69_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPI42886.1"
                     /translation="MAFSSFSRLSEDLIYRLGGDKYRSFIHIYLSWSKIVGEILASQS
                     HPVKVENGVLFVAVQNNTWMQELILIKDDIIAKYKSIYEEEIADIVFLISSPKRKCKR
                     RK"
     gene            complement(432..1007)
                     /locus_tag="PLG69_04925"
     CDS             complement(432..1007)
                     /locus_tag="PLG69_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42887.1"
                     /translation="MSDLWFKIKQIITLVVFVAVLSLLGMISGRPIMIVAYGVFFLVV
                     VAIMFYMTRKRQRHFDKVKGSSQLFRKIFGILLMILALITPPVIILRTNLITLPETIK
                     SGAALGIVSGVTVLFIALTLLAVYFINYRGSQVSNRVIGYILYIIAAIVPGFLMSRVE
                     KTTIGIGSVYYVALIVLILSYSGFGLLSNKE"
     gene            complement(1026..1472)
                     /gene="rplI"
                     /locus_tag="PLG69_04930"
     CDS             complement(1026..1472)
                     /gene="rplI"
                     /locus_tag="PLG69_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPI42888.1"
                     /translation="MKVIMLKTIEKVGNQGDVVNVKRGYARNYLVPRGFALYATPANL
                     KNLNAIKNKLADEEQKLTEELKNLAEKIDSTKLVFMRKVDEHGTMFGSVSELDIIRSL
                     EEQGINIQKNDLEMDKHFKELGEFQVPIHLRKDIVCTLNISIEPEQ"
     gene            complement(1469..2305)
                     /locus_tag="PLG69_04935"
     CDS             complement(1469..2305)
                     /locus_tag="PLG69_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42889.1"
                     /translation="MPIYPVIFGALSLLSPFLGLILYMLYVAKTAELNLTKPLQAFIM
                     FIPIPVLLLIFDPQTSTRLLCLDAILAVGLPILVFLIVLKNNHILSNSFLAAFLVMFA
                     WGMLRYYLFKTYQDQLFEQGMQVVKDKMPALLNNDLLQQTLPFWKGIIPSIWVISQAI
                     AWLIGFLLFEGRLQIPNRIENLRFPVYFNLLIIAVLPLYFFDQTKLLFYNLLLSLCII
                     PFFQGAGLVWQRLGMIFSNRIISGLFMLIIVLYANILLVLLGFGNMWFTKRNITPGGY
                     TE"
     gene            complement(2306..2542)
                     /gene="rpsR"
                     /locus_tag="PLG69_04940"
     CDS             complement(2306..2542)
                     /gene="rpsR"
                     /locus_tag="PLG69_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPI42890.1"
                     /translation="MNTSDRKKKYTRRKYCRFCANKEIQIDYKNIDVLKPYISEVGKI
                     EPARLTGTCSKHQRKLTTEIKRARQMALIPYVIE"
     gene            complement(2552..2995)
                     /gene="ssb"
                     /locus_tag="PLG69_04945"
     CDS             complement(2552..2995)
                     /gene="ssb"
                     /locus_tag="PLG69_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPI42891.1"
                     /translation="MADLKVPRLNKCIFSGRIGNDVELKYTPKGVPVVRFVVACDRRY
                     KDETEQWQTATSWIDCVAWRKWAEFIANQTHKGSAVIVEGRIETRNWTDQNNQNRKTT
                     EVIVDVLHSLEWKPREEGATQPNEEVPLPAEETLHHNTTTDDVPF"
     gene            complement(3005..3292)
                     /gene="rpsF"
                     /locus_tag="PLG69_04950"
     CDS             complement(3005..3292)
                     /gene="rpsF"
                     /locus_tag="PLG69_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPI42892.1"
                     /translation="MTKDYELMVILVPTLSADEANAMNDSLMALVTENGGEIIKIDPW
                     GKRMLAYPIKKFREGYYFVDYFRVDSLVIKKLKALFNINENIIRYLFVAKN"
     gene            complement(3292..3888)
                     /gene="pth"
                     /locus_tag="PLG69_04955"
     CDS             complement(3292..3888)
                     /gene="pth"
                     /locus_tag="PLG69_04955"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPI42893.1"
                     /translation="MKLIIGLGNPGKEFANNRHNLGFICLDRWALGRERNFAKGDVFD
                     YIVLKRAVLIKPKTWMNRSGEAIKQALKQWIISEYMVIYDDIELPVAKIRIRQGGGDG
                     GHNGIKSLLAVIPPEELKRIRIGIGRNNEIDPQNYVLSDILPEEWELLNPALDLAGKF
                     IDLYIKYDFNAVLNEYSIWKKSCSGAESSGIVSPKEEK"
     gene            complement(3898..4515)
                     /locus_tag="PLG69_04960"
     CDS             complement(3898..4515)
                     /locus_tag="PLG69_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPI42894.1"
                     /translation="MIFNLKATPRKTIRKSDLHTLRASGMIPAVIYGPELESISVSIP
                     KGDFTQLYKKSFAEVSFWDIELNGDQFHTILKDKQIHPVTREMLHLDFMVVSAGAIIE
                     VEVPIIITGEPIGVKEGGMMEIVQRNVKISCKANEIPDELTLDVSNLKIGESLHIRDL
                     PQGNWQVKEHSDVTLVAIHAPRAEVVEEKPAAPAPEEQTTPETEK"
     gene            complement(4531..5478)
                     /locus_tag="PLG69_04965"
     CDS             complement(4531..5478)
                     /locus_tag="PLG69_04965"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPI42895.1"
                     /translation="MFSKLILITGNANRPLAEEVSRHAGIPLADIDLFKFSNDESFVK
                     INDNVRGADVFVIQPTCYPVNDNLIDLLIIIDALKRASAQRINCVIPYYAYARSDKKD
                     QPRVPITAKLVADLITIAGAHRVITVDLHADQIQGFFNIPVDHLYAIPTFARYFKSIM
                     PMDDIVVVSPDSGGANRARALAKRLNCSLAIGDKRRSGNEDRAELLNIIGEVNNKTVI
                     LFDDIIDTGGSLIKVANALVDKGVKKIYAACTHGVLSGNAISDLEASPIEKLFITNTI
                     PLSEEKKQCTKIVQLSIAEMLAIAIKKVHIEESISVLFR"
     gene            5948..8974
                     /locus_tag="PLG69_04970"
     CDS             5948..8974
                     /locus_tag="PLG69_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPI42896.1"
                     /translation="MKKIAIVLCFALLTIGLFATLEWTEAVAIRQGVNIEWFRTGIET
                     TDGGAIYVWSDTKLGERDLWAQKVDGAGNMVWGEPVLIDGKPDRQEDPVITRTNDNNY
                     VIAWIDFSADLDGDVYAQKIDGEGNLLWQEGGVPVCTLVGMQIALNMEPDNEGGVYII
                     WVDSRNPSKDLFGQRLSANGSPLWIINGIPIATGLGDEMQNTMLPDGEGGMIIAYTHS
                     YAGNDDLYAKRFNANGTMVWQNTLVISEAEGSQSDIRMAALNDGNFVFTWADKRSADT
                     DIYAQKINLAGDLLWGSYLIVYSDQNGLARPQVNPRIVKTSDNGVIIVWEDFRLDTQN
                     PDLFAQKISASGIKQWSEEGIALCTAEFAQLGPRLASDNNGGCFVVWDDLRNGNAPNV
                     DIYAQHLSASGNALWEANGKAICIAPNEQSGSLIKVSNNIVFINWMDIRNGSVGIYYQ
                     ALTYEGTVLLAVNGAEVFWGLSGDAPLDNYLILKRSSDVVIIWQDTRFANDGYRLFFQ
                     FLNPDGSIDLEPNGHPVTVSVGGSQSTPSAVVTPDDQIAIIWEDARGDNPKVYAQLLS
                     ANGERLWGEQGMELTVNSPLRQKDPKVSYYNGSLYFGWSGSDQVETSFFYHIYGQRIY
                     NGQKMWGPNGILISTLAQSDLRNECTLYDLIDNYYVWHRINPALATQTIWVKRVAEDG
                     SAMDGWQEEGIQTSNYNDWDAIQLFPKAHKTPEGIFVMWRDLRNDYIQNYWGQHLAEN
                     GTYLWNPVGVNLNDNQREQEKASIVVNSSGITFAWCENINGMNDIMAQKYSFPGEPLW
                     TNSGYYVVQRDSAQSDPCLVYFPDGNYLISWADFLNIESDLYYKYITENGEMIGAPQG
                     NVLCNASKSQYQPQTAILNNKAYTVWADGRSSGKTEILGLYAQKLGNGTPVTDVTIPP
                     LQSLILHQNYPNPFNPETTISFTLKDTTLSLKLNIYNIKGQLVKTLYDGALQKGQHSF
                     VWNGTDETNCQVSSGVYFYRLSNGKESRQRKMVLMK"
     gene            complement(9529..10782)
                     /gene="murA"
                     /locus_tag="PLG69_04975"
     CDS             complement(9529..10782)
                     /gene="murA"
                     /locus_tag="PLG69_04975"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPI42897.1"
                     /translation="MERFIIEGNRNLNGTIYVSGAKNAVLPAMSACLLTKGKSVLHNV
                     PDLIDIKTMSHLLRIIGARVDFEDGSMSIDSRDVCFPEAPYELVSKMRASIYVLGPLL
                     ARLKEARVSFPGGCAIGTRPIDLHLRAMETLGAEISVDYGYINAKAKELKGADIYFTK
                     SSVGATINALMVAVLAKGKTRIFNAAMEPEVDSTIDLLNKMGAKINGKGTTTLEIEGV
                     EELIPVEMNMIPDRIEAGTFLIAGALSKNPVTVANCESAHLTAVLEKLRESGCELEIE
                     DKAITVIPPFSIKPVNLITLPYPGFPTDLQAQFTVLMSLADGVSFIEDTIFPERYMHI
                     AELNRLQANIKIERNIAAVKGVKELSGAEVMATDLRASAALVLAGMVAKGTTIVSRIY
                     HIDRGYEHIETKLNSIGANITREEV"
     gene            complement(10795..11616)
                     /gene="prmC"
                     /locus_tag="PLG69_04980"
     CDS             complement(10795..11616)
                     /gene="prmC"
                     /locus_tag="PLG69_04980"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPI42898.1"
                     /translation="MILQELLEQAKSLALAQNIPETDFWFLISYYLHLSRSEIILSRQ
                     RILTDREREIIENAFSRLEKGEPPQYITGTAYFYGLDLKVNPAVLIPRPETERLVELT
                     IERLKGTERILDIGTGSGAIAIALKHNLPSLNVSATEISFSALETAKKNAEIYGADIH
                     FYLSDCFPPVKQNYEVLISNPPYISKAEFATLNSRIKDKEPVIALQGGEDGLDFYRKL
                     LSESSEYLSENGFLALEHSDTQKEAIMNLARKEGWTKIEPLKDLTDKDRYLFIYR"
     gene            complement(11613..11732)
                     /locus_tag="PLG69_04985"
     CDS             complement(11613..11732)
                     /locus_tag="PLG69_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42899.1"
                     /translation="MEENGRGIAMLYHLSDKLEFDREGRTVTISKKLPINNKK"
BASE COUNT         3529 a         2334 c         2206 g         3668 t
ORIGIN      
        1 aatttcggaa tcaagatttc caaaatccgc tgctaagagt gaaggggcta tttgcgtctt
       61 ttgcatttgc gtttgggact gctgataaga aaaacaatat cagcaatctc ctcctcatag
      121 atagatttgt atttggcaat tatatcatct ttaatcagaa taagttcctg catccaagtg
      181 ttattttgaa cagcaacaaa aagaacaccg ttttccactt ttacaggatg cgattgggaa
      241 gccagaattt cgccaactat tttgctccag gaaagataaa tatggataaa agaccggtat
      301 ttatctccgc ctaaacggta gataaggtct tcggaaagcc gggaaaaaga gctgaaagcc
      361 attgctaaaa taaaactctc caccaaagat aaaacacttc ggtgaactta taatgtcctg
      421 tcaaaaaatg attattcctt gttggataat aacccgaaac cgctgtagga aaggattaaa
      481 acaatcagcg ctacataata tacggaaccg atgccaatgg ttgtcttttc cactctgctc
      541 attaaaaatc ccggaacaat agccgctata atataaagaa tataaccgat aactctgttg
      601 ctgacctgac tgccacgata attaatgaaa taaacagcca ataatgttaa agcaataaac
      661 agaactgtaa ctccgcttac aattcctaac gcggctcctg attttatcgt ctcgggaaga
      721 gttatcaaat tggtgcgcaa aataattacc ggtggagtta ttaaagccag aatcattaga
      781 agaattccaa aaatctttct aaagagttga gacgaccctt taaccttatc aaaatgacgc
      841 tgacgcttgc gtgtcatata aaacataatg gctaccacca ccagaaaaaa gacaccataa
      901 gcaacaatca taatgggtct gccgctaatc atacccaata gggataaaac cgcaacaaac
      961 actaccaaag tgattatttg tttaatctta aaccataaat cggacataaa aacctcccgg
     1021 ctttattatt gttccggctc aatagaaata ttaagcgtgc acacaatatc tttccgcaga
     1081 tgaataggca cctgaaattc gcccagttct ttaaaatgtt tgtccatttc caggtcattt
     1141 ttctgaatat taattccctg ttcttccaag ctacggataa tatccagctc ggatactgaa
     1201 ccgaacatag taccgtgttc atcaactttt ctcataaata ccagtttcgt ggagtcaatt
     1261 ttttcggcaa ggttttttaa ttcttcggtc aatttttgct cttcatcggc aagcttattt
     1321 ttaatagcat taagattttt caagtttgcc ggagttgcat ataaagcaaa gccgcgggga
     1381 acaagataat ttctggcata tcctctttta acattaacaa catcaccctg atttcccact
     1441 ttttctattg ttttcaacat aataactttc attccgtgta acctccagga gttatatttc
     1501 tttttgtaaa ccacatattt ccaaagccca acagaaccag gagaatattg gcataaagaa
     1561 caataataag cataaataag ccgctgatta tacggttgga aaaaatcatt cccaaacgct
     1621 gccaaaccaa acctgctccc tgaaaaaagg gaattatgca caaggatagc aacaggttat
     1681 aaaacagcaa cttcgtctgg tcaaaaaaat ataagggcaa gaccgcaatg ataagcaggt
     1741 taaaatatac cggaaacctt aagttctcaa tcctattagg tatttgtaac cttccctcaa
     1801 aaagcagaaa tccgatcagc caggcaattg cttgtgaaat aacccaaata gaaggtatta
     1861 ttcccttcca gaaaggtaag gtctgctgca acaaatcatt attcagcaaa gccggcattt
     1921 tgtccttcac cacttgcatt ccctgttcaa aaagctggtc ttgataggtt ttgaataaat
     1981 aatacctaag cattccccaa gcaaacatta ctaaaaatgc tgccagaaag gaattgctga
     2041 gaatatgatt gttttttagc acaattagaa aaaccaaaat cggcaaaccc actgccaaaa
     2101 tggcatccaa acacagtaaa cgggtacttg tttgagggtc aaaaatcaac agcagcaccg
     2161 gaatcggaat aaacattata aatgcctgca agggcttggt taaattcaac tcagccgttt
     2221 tggcaacata gagcatatac agaattaaac ccagaaaagg acttaatagt gataaagctc
     2281 caaagatgac aggataaatc ggcatttatt caattacata aggaattaat gccatctgac
     2341 gtgcacgttt gatttcagtt gtaagcttcc tttggtgctt ggaacaagtt ccggtcaaac
     2401 gtgctggctc aatttttcct acttctgaga tataaggttt cagaacatca atattcttat
     2461 agtcaatctg aatttcttta ttggcgcaaa acctgcagta ttttctgcgg gtatattttt
     2521 tctttctgtc ggaagtattc attttttacc tctaaaaagg aacatcatca gttgtagtat
     2581 tgtgatggag tgtttcttct gcgggaagag gaacttcttc atttggctgt gttgctcctt
     2641 cttcgcgagg tttccattcc agggaatgaa gaacatctac aatcacttcg gtagttttgc
     2701 gattttgatt gttttggtct gtccaattgc gagtttcaat tcttccttcc acaattacag
     2761 cgcttccttt atgagtttga ttagcaataa actcagccca tttacgccat gctacacaat
     2821 caatccaact ggttgcagtt tgccattgtt cggtttcatc tttgtaacgg cgatcgcagg
     2881 caacaacaaa acgaactaca gggactcctt taggggtata tttcagttct acatcgttac
     2941 caattctgcc gctaaagata catttgttta aacgcggtac tttcaaatca gccatttttt
     3001 ctcctcaatt ctttgccaca aataagtagc gaataatgtt ttcgttaata ttgaatagtg
     3061 cttttagttt cttaataacc aagctatcaa ctctaaaata atccacaaag taatagccct
     3121 ctctgaattt tttaatggga taagccagca tacgcttgcc ccaagggtca attttgatga
     3181 tttctccacc gttttcggtt accaaagcca ttaaactatc attcatagca ttagcttcat
     3241 ccgcacttaa ggtgggaact aaaatcacca ttagttcata atcctttgtc actatttttc
     3301 ctcctttgga cttacgattc cggaactttc tgctccggaa caggattttt tccagatact
     3361 atattcattc aacacagcgt tgaaatcata ctttatataa aggtctataa atttccccgc
     3421 caggtccaga gccggattca gcaattccca ttcttcgggt aaaatatcgc ttagcacata
     3481 attttgggga tcaatttcgt tgtttctgcc aattccaatc cgaattcttt tcagctcttc
     3541 cgggggtata actgcaagca aggatttgat gccattatga ccgccatctc ctcccccctg
     3601 acgaatacga attttggcta caggcagttc tatatcatca taaataacca tatattcaga
     3661 aattatccat tgctttaatg cctgttttat tgcttcccca gaacggttca tccaggtttt
     3721 gggtttaatc aaaacagctc gtttaagaac aatataatca aaaacatcac ctttggcaaa
     3781 attcctttct ctgcctaaag cccaacggtc aagacagata aaacccagat tgtgacggtt
     3841 gttggcaaat tcctttcccg gatttccaag tcctataatg agcttcataa ataagcatta
     3901 tttttctgtt tcgggagttg tctgttcctc aggagccgga gcagcaggtt tttcttctac
     3961 aacttctgct ctgggagcgt gaattgctac taaagttaca tcgctgtgtt cttttacctg
     4021 ccagttacct tggggaagat cgcgaatatg aagtgactcg ccaattttta aattgcttac
     4081 atctaaagtc aactcatcag gaatttcatt cgctttacaa gagattttta cattgcgttg
     4141 cacgatttcc atcattccac cttctttaac tccaattggt tctcctgtaa taataatcgg
     4201 aacttccact tcaataattg ccccggcaga aacaaccata aaatccaaat gcaacatttc
     4261 cctggtgaca ggatgaatct gcttatcctt cagaatcgta tgaaattgat caccatttaa
     4321 ttcaatatcc cagaaactta cttccgcaaa acttttctta tacagctgcg taaaatcccc
     4381 tttagggatg cttacagaaa tactttccag ttcaggtccg tagattacgg caggtatcat
     4441 tccactcgct ctcaaggtat gcaaatcgga tttcctgatt gtttttctgg gagtggcttt
     4501 caggttaaat atcatttatt cctccattta ttatctgaat aaaacactga ttgattcttc
     4561 aatgtgcact ttcttaattg ctattgctag catttcggca atagacaact gcactatttt
     4621 ggtgcattgt ttcttctctt ctgaaagagg tattgtattg gtaataaaga gtttctctat
     4681 tggagacgct tccagatcgg atattgcatt accgcttaga acaccatgtg tgcaagcagc
     4741 ataaatcttc ttaactcctt tatctactaa ggcattagct actttaatca aacttccgcc
     4801 cgtgtctata atatcatcaa ataaaataac cgttttatta tttacttcac cgataatatt
     4861 taagagctcg gctctatctt catttccgct tctgcgttta tcgccaatgg ctaaactgca
     4921 attcaagcgc tttgccaaag ctcttgctct gtttgcacct ccggaatcgg gagaaacaac
     4981 aactatatca tccattggca taatactttt aaaataacgg gcaaaagttg gaatcgcata
     5041 taaatgatcc acaggaatat tgaaaaaacc ctgaatctga tcggcatgca aatccacagt
     5101 aataactctg tgcgcacctg caatagtaat taaatccgct accagctttg ctgtaatagg
     5161 aactcgcggc tgatcctttt tatcggaacg cgcataagca taataaggaa taacacagtt
     5221 tatccgctgt gccgaagctc tctttaaggc atctataata attaataggt caatcaaatt
     5281 atcattcaca ggataacaag tgggctgaat tacaaaaaca tcagctcccc gcacattgtc
     5341 attgattttt acaaacgatt cgtcattgga gaatttgaat aaatcaatat ccgccaaagg
     5401 aattcccgca tgccgggaaa cttcttctgc caaaggacga ttcgcatttc ctgtgattaa
     5461 tattagctta gaaaacactt ttctctcttc tcttattttt gcttgttcca gttaaatttc
     5521 ctctttctgc atatccattg cggctatgca cttaccatat cctcttacct gataattttt
     5581 atttcccata acctcatata taatccaata cctaagctcg taattcctct atcaactaac
     5641 ataatttcta tatcttacat cgctttctac ctgtctattc aggcaagttt taccattttt
     5701 ttaggcaagc tcattttgtc aaagcttatt tatcccctta caatataaac ttgtttggaa
     5761 gaattcttgc ataaatgtat gcttggtgga gaatatattt tcagttgtta aaggagaaat
     5821 gtttttcagc attgaggcag aggatgatta gagaaatgca tttgaagtga aaggttatat
     5881 tcttcaatgt attatagccc aaaagcggta taaaaaatag tttttttatt aaaagaagga
     5941 gatagaaatg aagaagatag cgatagtgct ttgttttgcc ctgcttacta tagggctttt
     6001 tgccaccttg gaatggactg aagcagtggc aattcgtcaa ggggtgaata ttgaatggtt
     6061 tcggactggc atagaaacca cagatggtgg agccatatat gtttggtctg atacaaaact
     6121 tggcgagcga gacctttggg ctcagaaagt tgatggtgca ggaaatatgg tttggggaga
     6181 gccggtttta atagatggca agccagaccg tcaggaagac cctgtaataa ccagaacgaa
     6241 tgataataat tatgttattg cctggattga tttttctgcc gatttggatg gtgatgttta
     6301 tgctcaaaag atagatggag agggtaattt gctttggcag gaaggcggag ttcctgtttg
     6361 tactctggtg ggaatgcaga ttgctcttaa tatggagccc gataatgaag gtggtgttta
     6421 tataatttgg gtggatagtc gcaatccgag taaagacctt tttggacagc gtctttctgc
     6481 aaatggcagt cctttatgga taataaacgg aattcctatt gccactggct tgggggatga
     6541 aatgcaaaat acaatgcttc ctgatggcga gggagggatg attattgctt acacacacag
     6601 ttatgccggt aatgatgatc tttatgccaa gcgttttaat gccaatggaa ctatggtttg
     6661 gcaaaatact ttagttatca gtgaagcgga aggaagtcaa tccgacatta gaatggctgc
     6721 tctaaatgat ggtaattttg tttttacctg ggctgataaa cgcagtgccg ataccgatat
     6781 ttatgcccaa aagattaacc tggcagggga tttgctttgg ggtagttatt taatagttta
     6841 cagtgatcaa aacgggcttg cgaggcctca agtgaatcct cgtattgtaa aaacctccga
     6901 taatggagta atcattgttt gggaagattt tcgtttggac acacagaatc ctgatctttt
     6961 tgcgcaaaag atttctgctt ccgggatcaa acaatggagt gaagagggaa ttgctttatg
     7021 cacggcagaa tttgctcaat taggtcctcg tctggcttct gataataatg gcggttgttt
     7081 tgttgtttgg gatgatttaa gaaacggaaa tgctcccaat gtagatattt atgcacagca
     7141 tctttctgct agtggaaatg ctttatggga agcgaatggc aaggcaattt gtatagctcc
     7201 caatgagcaa agcggtagtt taattaaagt aagtaataat attgttttca ttaactggat
     7261 ggatattcgt aatggaagtg ttggtattta ttatcaagcg cttacttatg aaggtactgt
     7321 tcttctggca gtaaatggag ctgaggtttt ttggggttta agtggagacg cacctttaga
     7381 taattatttg attttaaaac gcagttccga tgttgttatt atttggcagg atacccgttt
     7441 tgccaatgac ggctatcgtc tttttttcca gtttcttaat cctgacggca gtattgattt
     7501 ggaacccaat ggacatcctg taactgtttc cgtgggagga tcacaatcca ctcccagtgc
     7561 agttgtaact cctgatgacc aaattgccat tatttgggaa gatgccagag gcgataatcc
     7621 taaggtttat gctcaacttc ttagtgccaa cggagaaaga ttatggggag agcagggtat
     7681 ggaacttacc gttaatagtc ctttaaggca aaaagacccg aaagtaagtt actataacgg
     7741 ttccctttat tttggctggt ccggttccga tcaagtggaa accagtttct tttatcatat
     7801 ctacggacag cgaatttata acggacaaaa aatgtgggga ccgaatggaa tactaatttc
     7861 tacccttgct caaagtgatt taagaaatga gtgcactctt tatgacctga tagataatta
     7921 ctatgtttgg catagaatta atcccgctct ggcaacacaa actatttggg tgaaaagagt
     7981 ggcagaagat ggttccgcta tggatggatg gcaggaagaa ggaattcaaa cctctaatta
     8041 taacgattgg gatgctattc aactgtttcc gaaagctcat aagacacctg aaggtatttt
     8101 tgtaatgtgg agggacttac gtaacgacta tattcagaat tattggggac agcatcttgc
     8161 ggaaaacgga acttatttat ggaatccggt aggggttaat cttaacgata accaaagaga
     8221 acaagaaaag gcatctattg ttgttaacag cagtggcata acttttgcct ggtgtgaaaa
     8281 cataaacgga atgaatgata taatggctca gaaatattct tttccgggtg aacctttatg
     8341 gaccaatagc ggctattatg ttgttcaaag ggattccgct caatcagacc cctgtcttgt
     8401 ttattttccc gatggtaatt atcttatttc ctgggctgat tttttgaata tagaaagtga
     8461 cctttattat aaatacatca ccgaaaatgg agaaatgatt ggagctcctc agggcaatgt
     8521 tctttgtaat gcttctaaat cgcaatatca acctcaaact gctattctga ataacaaagc
     8581 atatactgtt tgggcagatg gacgttccag cgggaaaacc gaaattttgg gactttatgc
     8641 tcaaaaattg ggtaatggaa cacctgttac cgatgtaaca atacccccgt tgcaaagttt
     8701 gattttacac cagaattatc ccaatccctt caatcctgaa actacaatca gcttcacttt
     8761 gaaagatact accctttccc taaaactcaa tatctataat ataaaagggc aattggtaaa
     8821 aaccctttat gatggagcgc tgcaaaaagg acagcatagt tttgtttgga atggaacgga
     8881 tgaaacgaat tgccaggtct ccagcggagt ttatttttac cggctaagta acggaaaaga
     8941 aagcagacaa cggaaaatgg tgctgatgaa ataagtagag aggtcaagag gtagagaaat
     9001 caaaaaataa aaaggtcttg agattaagag ggaaaaaggg ctttggagct tggggtgaga
     9061 gttaaatttg aatatggaaa ttaaagcaaa aatgggttgg caaattccga tttgccacag
     9121 atgatatagt catctaaaga aactttatcc ttcgtattta ccgaatcgga atttggcaac
     9181 ccaagggttc acgattgtta cagctgaatt ttaatgttta ttttggcatt aactatatat
     9241 ttgtttatat tccaagctat tataccgttt tccactgcct tccctaatag cattgcttac
     9301 ccattgctta cccattacta atgccatccg tattgggtaa gcaatgagtg agtaatgcat
     9361 tcggggatag caagttccat tgtaaatttt attatgactg ccgagttaca tttatgcttc
     9421 atttttcatt cttccaacat agtagctatg agtcctaact ttattatgta gttggagttt
     9481 accgtagttc cgctttttaa tttttagcgg aggaacagtg tcctccggct acacttcttc
     9541 gcgggtaata ttggctccaa tggaattcag ttttgtttct atatgttcat aaccgcggtc
     9601 aatatgataa attctggaaa caatagtagt gcctttagct accatacctg ccaaaacaag
     9661 agctgcactt gccctcaggt ctgtagccat aacttctgct ccactaagtt ctttcacacc
     9721 tttaacggca gctatatttc tttcaatttt aatatttgct tgcagacgat tcaattctgc
     9781 aatgtgcata taacgttcag gaaaaattgt atcttcaata aagctaaccc catcagccag
     9841 agacataaga acggtaaatt gagcttgtaa atcggttgga aaaccgggat aaggaagagt
     9901 tattaaattt acgggcttaa tggaaaaagg tggaataacg gttatggctt tatcttcaat
     9961 ttccagttca caaccgcttt cccgtaattt ttccagaact gcggttaaat gtgccgattc
    10021 acaattagct actgtaacag ggtttttact aagagcacca gcaatgagaa aagttccggc
    10081 ttcaattcta tcgggaatca tattcatttc caccggaatg agttcttcca caccttcaat
    10141 ttccagagta gtagttcctt taccgtttat ttttgctccc attttgttta ggaggtcaat
    10201 agtggaatcc acttctggtt ccattgcagc attaaatatt cttgttttcc ctttcgccag
    10261 aacagctacc atcagagcat taattgtagc tcctacgctg gatttggtaa aatagatatc
    10321 agcgcctttc agttcttttg ctttggcatt aatatagcca taatcaacgg atatttcggc
    10381 tcccagggtt tccattgccc ttaaatgaag gtcaataggt ctggtgccaa tggcacaacc
    10441 accaggaaaa gataccctgg cttctttcaa acgcgctaaa agaggtccta aaacatagat
    10501 ggaagcgcgc attttgctga ccaattcata aggggcttcc ggaaaacaaa catcccggga
    10561 atcaatactc atactgccat cttcaaaatc cactctggct ccgattattc ttaataaatg
    10621 ggacatcgtt ttgatgtcta tcaaatcggg aacattatgc aacaccgatt tccctttagt
    10681 taaaagacag gcagacattg caggtaaaac agcattcttg gctccgctta cataaatagt
    10741 gccattaagg ttacggtttc cttcaataat aaatcgttcc attttttatc ctttttacct
    10801 gtaaataaac aggtatctat ccttatcggt caagtccttt aagggctcaa tttttgtcca
    10861 gccctctttt cgggcaaggt tcataattgc ttccttttgg gtatcgctat gctctaaagc
    10921 taaaaaaccg ttttcggaaa ggtattcaga gctttcagag agtaatttac ggtaaaaatc
    10981 aagtccatct tctccacctt gcaaagcaat tacgggttct ttatctttaa ttctggaatt
    11041 tagagtagca aattctgctt tactgatata aggaggattg gaaataagca cttcgtaatt
    11101 ctgtttaaca ggaggaaaac aatcggacaa ataaaaatga atatctgccc catatatttc
    11161 agcattcttt ttagctgttt ccaatgccga aaagcttatc tccgtagcag aaacatttag
    11221 agaaggtaga ttatgtttta aggcaatagc aatagcgcca ctacctgtgc caatatccaa
    11281 aattctttct gttcctttta acctttctat tgtcaattcc accagccgtt ccgtttccgg
    11341 acggggaatt aaaactgcag gattaacctt taggtctaaa ccgtaaaaat aagctgttcc
    11401 tgttatatat tgaggtggtt ctcctttttc caaacggctg aaggcatttt caattatttc
    11461 cctttcccgg tcagttaaaa tccgttgtcg ggataaaata atctcactcc ggctgagatg
    11521 caaataatag gatattaaaa accagaaatc cgtctcagga atattttggg ctaaagctaa
    11581 actttttgcc tgttccagca actcctgtaa aatcattttt tattgtttat gggcagtttt
    11641 ttactgatag taaccgttct gccttctctg tcaaactcca gtttgtcgga aagatgataa
    11701 agcatcgcaa ttcctctgcc attttcttcc agattca
//
LOCUS       DAOPFL010000052        11245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_6986_length_11245_cov_59.697230, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000052 DAOPFL010000000
VERSION     DAOPFL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6986_length_11245_cov_59.697230"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..5777
                     /locus_tag="PLG69_04990"
     CDS             75..5777
                     /locus_tag="PLG69_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPI42900.1"
                     /translation="MHATVTKFVPGDYATVAQAVTWLNAEGALTDNYVINVYADHTET
                     LSARIELTATGTSSYSITIQKYGTGANPKLTAYVGTSTPGSAIPDGMFCLKGADYVTI
                     DGIDLYDPNTTNPATMEYGYGLFKSSISDGAQYNTIKNCNVTLSRNNFASGTSPMVEG
                     SVCILVINSTPTAATTALTPSVATGTNSFNKFYANTLSNANYGIALSGYAAASPFTLG
                     DTGNDIGGNTVSTGNTIINYGGGSGSSNPSAGIRANNQWGINISYNQINNNDGNGVNH
                     PSTLRGIYAQAGTSANCDINHNTITIKGGGTTSQLSAIECAIGSTAASNTVNVNYNTI
                     TNCTYTTATSGTFYGIYVSSTPAYTNINYNIVSNNSLAGTGSWYGIYNSNSTNTTTAT
                     INYNQIYSNSKTASGYMYCLRPNTAQTTANYNEIHDNTITSSGTASGNLYGLYNFGSP
                     TVENYNNNQIYNLNIDGTSTSTSSTIYALYTSTTSSSIKTINKNQIYNIQYTASGAAT
                     IYGIYHSSGTTINIGQNLIHNFWAAGASSSVNGIYISSGINNNISNNMIWALNAAGST
                     SSVTNSVNGIRITGGTTNNVHFNTVFLNGTGTNSYYSTAALYISSGTTNEIRNNIFKN
                     VSTPGVNGYTVAIWKTTAGTSNLGSNSNKNIYYSGEPGEHNIIAYINSVGYRTLQDYK
                     NLLVDRDQGSYTEDVQFISEEDLHINPDIATRVESNAVAILGCDIDIDDEDRSDTNPD
                     IGADEGDFTLVIAAPEAPIYVSPTNGASGVDIYTPLVWSANSEGGTPTNYDVYFSEDS
                     DPAYFDNVLTTTCNPPKQYSKTYYWKVVAKNDQGSAEGVIWSFTTMADPNFYVTADSP
                     YLMDFEEITTAGELPQGWTKAGTKWNTATTTGTYNRAPRSGTDYATCGYSATTSDWLF
                     SRPLYLDATKNYDFGIWYNTDGYTGWTSFKMYIGNSATGSAMTTELASVSNPINTSYM
                     QLTKTAWQPNTSGSYVVGLQVIATSSPWYMSFDDFSVSETPLNPVFTINPTSKDFGLK
                     QVYTQTDQLFTISNTGQGTLTISDIQYEGDNCFSVINLPQFPVNLTAGQNTTFIVRYI
                     PTTTGDFSGQVKITDNLAEKSLRTVSVTGSAFDPILPLPYTQDFNASTNFPANWTQST
                     SQWSIGSSHGRTGNGIYKNIWSSATNAWFQTQPIGPITEHTKLSFYYRLVNYSSYPST
                     AFIPGTGDNIKIWVSTDAGTTFTQYGEINSSNHTPTINWTEKSIWLSGMKASAGDRII
                     LKFEANWGSGDWFIDIDDIEVKDVPANPVFSITPESKAFGELQINTVSAPQVFTIKND
                     GGGTLIINPAVELTGTNADQFQLTDTNSYPCNLGPDQTITVSVAFAPTSLGEKTASLK
                     IVDNLSKTEHNIPLTGTGVDYTIYVPYLNDFQSDINGWTILDVNNDGKKWTLTYEISP
                     NKAMKIVYNSSLAMDDWLIAPPISLTKDTTYDIKYEYRVQSTSFPENLGVYIGNSPTP
                     AALTNLLNDHPGITNTTFSLGQATFTPQTTGIYYIGFHGYSDADMYNLYVDNFRITFT
                     NSEIVSGTASDEGIANLDPPPINNPVTGNPLDTSLTITGITGTPTITATVAWAPPTVN
                     LPNVGLVFTLSATSSLAGVNVTFTHNLGFIPDQIAYRIIPGTYTLLNDPNDGSWTTTT
                     FTMVIPSGKADGDFEVVFPSEDNQTLPVTLSNFTAVVTSENFVNIAWMAETETNHSGY
                     NIFRNETKDLENAIKINAQLIDKGTAVGTQISYFYTDFEVYTNMMYYYWLESVALDGS
                     SQFYGPLTVTIGDPAQEPLPPVIPMVTKLYNAFPNPFNPNTNIRYSLKEVGKVKIEIY
                     NMKGQKIKTFTQEHNSPGYYQVSWDGRDENGRSVASGIYLYRLTTANYTSAKKMVLAK
                     "
     gene            complement(6746..10324)
                     /locus_tag="PLG69_04995"
     CDS             complement(6746..10324)
                     /locus_tag="PLG69_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPI42901.1"
                     /translation="MKAQIYKLLLLLIFSVTAIAAFSTVSEYSFASTLGTFTEISGGT
                     IHGTSANDNECFCAIPLGFTFTYNGVDYTTISIASNGFIAMGDTVVTSNVPISSNTST
                     NNVIAVLARDLKSRADGTLMSLSSGTAPNRVFTIQWKNWRRTPTATANDDFTFQIQLQ
                     ENGNKVVFVYGPFTAVTAATSATVQVGLRGDSNNDFNNRLTTTDWSATTAGTANNSTC
                     TLSATVFPANGLTFTFAPPITGEPPLAAQNPHPANGAINVPIATNLSWNSGGGIVDGY
                     KVYFGTDNPPTNIVNGTTQTVTVYDPVNDLTYSTTYYWKIVPYNQFGDAINCPVWSFT
                     VLADPTVTTYPYEQNFDSVTPPDLALGWTVINANNDTYTWQSYNGNSQTSPNSMRVRY
                     NSTLAMDDWLFTPPLVFTNTHNYKIKFYYCSSSATYPEKLSVYWGTTPAVASMTNLLW
                     ENLNIINTAYEAAEIIMPAVTGGTYYIGFHGHSDADRFYLYIDTFSVSDIVEQINPPQ
                     NLTATIQNYYNVHLAWQAPVSSFGTRDLLGYKVYRDGSLINTISNPATLTYDDLGLEV
                     GTYSYTVTAYYTTGESVPAGPVSVEIVAPLDPPLNLTATVQNNTNVFLDWDAPGVVPP
                     PTGFSDGFETYTDFSISFAPWTLVDVDQSTTYGMQGISWTNAYAAQAYIIFNPSATTP
                     AITDLTAHTGNKMAACFASTVPPNNDWMITPQLSITAGNVLKFWARSYTDQYGLERFK
                     VGVSTTGTTPANFTIISGTSYIQAPVTWTEYTYDLSSYAGQNVYIGIQCVSNDAFIFL
                     VDDVFIGTPATAKSYPYVASVPGTIERVKGVSVPTIRQETKNNDRALLGYKVYRNGTL
                     ISTINNPTITDYTDLDLANGTYNYGVTALYTTGESVPATVSVTINYQPSPAFFTDDFE
                     SYANFVTTFAPWTTVDVDLADTYGIQDVDFPGSGDAMAYIIFNPSATVPPLTTLTAHS
                     GSKMPASFAATTPPNNDWMISPRVFLGTGSFVKFYARSYTAQYGLERFKVGVSTTTPN
                     PANFQFVSGATYVQAPVTWTEYTYDLSSYDNQNVYIGIQCLSNDAFIFFVDDVSIHGV
                     NGYDNNDNNAPVVQTELKGNYPNPFNPETTIAYSVKEHIPVNIEIYNIKGQKVKTLVN
                     ETKAPGNYTAVWRGLDDNNHPVSSGVYFFKMNAGKYSSTKKMIMMK"
BASE COUNT         3566 a         2151 c         2081 g         3447 t
ORIGIN      
        1 aaaaggagaa atctattatg aaaaagaata ttttactctt ctcattcctg gttatgttac
       61 taggaacaat ttgtatgcac gcgaccgtaa caaaatttgt cccgggtgat tatgctactg
      121 ttgcccaagc tgttacttgg ttaaatgctg aaggtgcttt aactgataat tatgtaatta
      181 atgtatacgc tgatcatacg gaaacattat cggcaagaat tgaacttact gctaccggca
      241 catcttctta ttctataacc attcaaaaat atggaactgg agccaatcct aaactaactg
      301 cttatgttgg tacatctaca ccaggatctg ctattccaga tggaatgttt tgtttaaaag
      361 gggctgatta tgttaccatt gatgggattg atttatatga tccgaatact accaatcctg
      421 ctacaatgga atatggatat ggtctcttta aaagtagtat cagtgatggt gcacaatata
      481 acactattaa aaattgcaat gtaactctaa gcagaaacaa ttttgccagt gggacctctc
      541 caatggtgga aggttctgtt tgtatcctgg taattaattc tacccctact gcagctacaa
      601 ctgcattaac accatcggtg gctacaggaa caaactcttt caataaattt tatgccaata
      661 cccttagtaa tgccaattat ggtattgccc tcagtggata tgcagctgcc tctcctttta
      721 cattaggtga tactggtaat gatattggag gcaatacagt ttctacaggt aacacaatta
      781 ttaattatgg cggtggctca ggttcttcta atccttctgc tggtatccga gcaaataatc
      841 aatgggggat aaatatatcc tataatcaga taaataataa tgatggcaac ggagtaaatc
      901 atccttcaac cttacgcgga atttatgctc aagccggaac cagtgccaat tgcgatatta
      961 accacaatac tataactatc aaaggtggcg gaactaccag tcagctttct gccattgaat
     1021 gtgcaatcgg ttctacagct gcttccaata ctgttaatgt aaactacaat acaattacta
     1081 actgtactta tacaacggca acctcaggta ctttttatgg aatttatgtt tcttccactc
     1141 cggcttatac aaacatcaat tacaatattg tgtccaataa ctctcttgct ggaacaggtt
     1201 cttggtatgg aatttataat tccaattcaa ccaatactac tactgctact attaattata
     1261 atcagattta tagtaattca aagacagcta gcggttatat gtattgccta agacccaata
     1321 ctgcacagac aactgctaac tacaacgaaa ttcacgataa tactattaca tcttcaggta
     1381 ctgcttctgg aaatctatat gggttatata actttggttc tccaaccgta gaaaactata
     1441 ataataacca aatttacaat ctcaacattg acggaacaag cacatcaact tcttctacaa
     1501 tatatgcttt gtatactagt acaacttctt ccagtataaa aacaataaat aaaaatcaga
     1561 tttacaatat ccagtatact gcttccggtg ctgcaactat ttatggtatt tatcattctt
     1621 cgggaactac aataaatatc ggacaaaact taatccataa tttctgggca gcaggtgcca
     1681 gttcatcagt aaatggtata tatatctcca gcggaattaa caataatatt tccaataata
     1741 tgatttgggc tcttaatgct gctggttcaa cttcatctgt aactaattcg gttaatggaa
     1801 tcagaatcac cggtgggaca acaaacaatg ttcattttaa tactgtattt ctgaatggaa
     1861 cgggaaccaa tagttattat tctactgctg ccctctatat tagcagtgga acaactaacg
     1921 aaatccgcaa taatattttt aaaaatgtca gcactcctgg tgttaatggt tacacggtcg
     1981 caatctggaa aacaactgct ggtacaagta atttgggtag taattccaat aaaaatattt
     2041 attatagcgg agagccaggt gaacataata taatagcata cattaattcc gtaggatatc
     2101 gcactcttca ggattataaa aatttattag tagatcgtga ccaaggttcc tatactgaag
     2161 atgttcaatt tataagcgaa gaagacctac acatcaatcc tgacattgct acccgcgtag
     2221 aaagtaatgc ggttgcgatt ttaggttgcg acattgatat tgatgatgag gatcgtagtg
     2281 atactaatcc cgatatcggc gctgatgaag gtgactttac tttagttata gcagctcctg
     2341 aagcaccaat atatgttagt cctacaaacg gggcttccgg tgttgatata tatactcctt
     2401 tggtatggtc tgctaattct gaaggtggaa ctcccaccaa ttatgatgta tatttcagtg
     2461 aggattcaga ccctgcatat tttgataatg ttcttaccac aacctgcaat ccccctaaac
     2521 aatatagtaa aacatactat tggaaagtag ttgctaaaaa tgatcagggc agtgctgaag
     2581 gtgttatttg gtcattcaca actatggctg atccgaattt ctatgtaact gctgatagtc
     2641 cttatttgat ggattttgaa gaaattacta ctgctggaga attaccccaa ggatggacaa
     2701 aagctggtac aaaatggaat accgctacaa ctaccggaac ttacaatcgt gctccccgta
     2761 gtggaactga ctatgctact tgtggttata gtgctactac ttctgattgg ctattctctc
     2821 gccctcttta ccttgatgca acaaaaaatt atgactttgg tatatggtat aatacagatg
     2881 gttatacagg ttggacttca ttcaagatgt atattggtaa ttcagcaact ggtagcgcaa
     2941 tgactactga attagcgtct gtttccaatc caattaatac ttcttatatg caactaacga
     3001 aaacagcttg gcaacctaat acttccggct cttatgttgt tgggctacag gtaattgcta
     3061 ccagcagtcc ctggtatatg agttttgatg atttttcggt atcggaaact cctctaaatc
     3121 ctgtatttac tataaatcct accagcaaag attttggttt aaaacaagta tatactcaaa
     3181 ctgatcagct ctttaccatt agtaatacag gtcaagggac tttaacaatt tccgacattc
     3241 aatatgaagg tgataattgt tttagtgtca taaatctacc acaatttccc gttaatttaa
     3301 cagcagggca aaacactaca tttattgtta gatacattcc tactacaaca ggtgattttt
     3361 ctggacaggt aaaaattacg gataatttag ctgaaaagtc cttaagaact gtgtcagtta
     3421 ccggttctgc ttttgatccg atattacctt tgccttatac tcaggatttc aatgcttcta
     3481 ctaatttccc tgcaaattgg actcaaagta catctcaatg gtctattggt agttctcatg
     3541 gaagaacagg aaatggaatt tataaaaata tctggagttc agctactaat gcttggttcc
     3601 agacccaacc cataggaccg ataactgaac ataccaaact tagcttctat tatcgcttag
     3661 taaactatag ttcatatcca agcactgctt tcattcctgg aacgggagat aatattaaaa
     3721 tttgggtttc aactgatgct ggaacaacct ttactcaata tggtgaaatc aatagttcca
     3781 atcacactcc aacaataaat tggactgaaa aatcaatctg gctgagtgga atgaaagcat
     3841 cagctggtga tcgtattatc ttaaaatttg aagccaactg gggctcaggt gattggttta
     3901 ttgatattga tgatattgaa gtgaaagatg tccctgctaa tcctgtcttt tctataactc
     3961 ctgaaagtaa ggcatttggc gaattacaga ttaatacggt ctctgcacct caggttttca
     4021 ccattaaaaa cgatggcggg ggaaccttaa ttatcaatcc tgctgttgaa ttaactggaa
     4081 ctaatgctga tcaattccag cttactgata ctaatagtta tccttgtaat ttaggtccag
     4141 atcagacaat tactgtctcc gttgcttttg ctcccacttc cctgggtgaa aaaacagcaa
     4201 gtttaaaaat agttgataat cttagtaaaa cagagcataa tattccccta accggaactg
     4261 gagttgatta tacaatttat gttccctatc tgaatgattt ccagagcgat atcaatggat
     4321 ggacaatatt agatgtaaac aatgatggga aaaaatggac tcttacttat gagatttctc
     4381 ctaataaagc aatgaagatt gtctacaatt cttctctggc aatggatgat tggcttattg
     4441 caccaccaat aagtttgact aaggatacta cctacgatat aaagtatgaa tatcgtgttc
     4501 aatcaacttc ttttcctgaa aatcttggtg tatatattgg caactcacct actcctgcag
     4561 ctcttactaa cttacttaat gatcatcccg gaattaccaa taccactttc agtttaggac
     4621 aagcaacctt tactcctcag acaacaggaa tttattatat tggtttccac ggttacagtg
     4681 atgctgatat gtataatctc tatgtagata atttccggat tacatttact aatagcgaaa
     4741 ttgtaagtgg aaccgcctct gatgaaggaa ttgctaatct tgatccacct ccaattaata
     4801 accccgttac gggaaaccct ctggatactt ctctaacaat aacaggtatt acaggcacac
     4861 caacaattac agcaactgtt gcttgggcac cacctacagt aaatctgccc aatgttggct
     4921 tagtatttac tttaagtgca acaagcagtt tagctggagt taatgtaact tttacccaca
     4981 atcttggttt tattcccgat caaattgcct atcggataat acctggaaca tatacattac
     5041 taaatgatcc taatgatgga tcgtggacaa ctacaacctt tacgatggta attccatccg
     5101 gtaaagcaga tggtgatttt gaagttgtat ttccttctga ggataatcag acactgcctg
     5161 taaccttatc caacttcaca gctgtggtaa cttcagaaaa ctttgtcaat attgcctgga
     5221 tggcagaaac cgaaaccaat cattctggtt ataatatttt ccggaatgaa acaaaggact
     5281 tggaaaatgc tatcaagatc aatgctcagt taattgacaa aggaactgcg gttggaactc
     5341 agatcagtta tttctacaca gattttgagg tctatactaa tatgatgtat tattactggc
     5401 tggaaagtgt agctctggat ggcagtagcc aattctatgg tcctttaacg gttactattg
     5461 gtgatccggc tcaggaacca cttcctccgg ttattccaat ggttactaaa ctctataatg
     5521 ccttccccaa tccattcaat ccgaatacca atattcgtta ttccctgaaa gaggtaggta
     5581 aggttaaaat tgaaatctat aatatgaaag gacaaaaaat caagaccttc actcaggaac
     5641 acaatagtcc aggttactat caggtttctt gggatggtcg tgatgaaaat ggaagaagcg
     5701 ttgccagtgg aatttatctg tatcgcttaa ctacagctaa ttatacatct gctaaaaaga
     5761 tggtattagc gaaataaagt taacgggtta actggttagc aagtttagtt tgaaggttaa
     5821 catgctaaca agtgatatag tggtcggagc tcttaaaaaa gctccgacta cttttttatc
     5881 tggcagaaag gcatcctgtc gtttcccctt caaccagatt acggagaaca agttccgtag
     5941 gaatgataga tattatagaa gggtttcaac ccagcgagga agaggagaat gaaaaaaggg
     6001 ctcacgcaga tttcgcagat gacgcagatt aaaaatgaga ataggtatgg gatgaacagg
     6061 atgatggaga ttatcaggat ttttatattc taaataatac agtgaggaga aaaagagctg
     6121 gaaaattccg atttgcctaa aaactgaagg tttctgcagg gacttagatt atttttagat
     6181 tgaaaaaatc ggatttatgc ctttgtttct ataatttctt tcctttacca ttcctgtgtt
     6241 ttatcattac agcattctta tcattcctgc gtttttgtca ttccagtgct tttatatttt
     6301 aacctatata atttaatttt tcaatatcta attcatacag gttggtcatc ctagataata
     6361 aaaatcctaa ttatccttat aatcctgttc atcccttacc tattattttt aaatctgcgt
     6421 aatctgcgta atctgcgtga aaccagccgt cttcattcct gctctcctat cattcctgtg
     6481 tttttgtcat tccagtgctt gtatatttta acctatataa tttaattttt caatatctaa
     6541 ttcatacagg ttggtcatcc tagataataa aaatcctaat tatccttata atcctgttca
     6601 tcccttacct attattttta aatctgcgta atctgcgtaa tctgcgtgaa accagccctg
     6661 actttaataa actgcaaaaa aaggcggagg aacaacgtcc tccgtctata tatcttattt
     6721 cttatcttac tatttcattc tacatttatt tcatcattat cattttcttc gtgctgctat
     6781 atttaccagc attcatttta aagaaataca ctccgctgga cacaggatgg ttattatcat
     6841 ccagtcctct ccacacagcg gtataattac cgggagcttt tgtttcgtta accagggttt
     6901 taactttctg acccttaatg ttataaatct caatatttac gggaatatgt tctttaacgc
     6961 tataggcaat agtggtttcc ggattgaacg gattgggata atttcctttc agctcagttt
     7021 ggactacagg agcattgtta tcattattat catatccatt tactccatga atgcttacat
     7081 cgtcaacaaa gaagataaag gcatcgttgg aaagacattg aattccaata taaacatttt
     7141 gattatcata actggatagg tcgtaagtat attcagtcca agtgacaggt gcctgtacat
     7201 aagttgcacc ggaaacaaat tggaagtttg caggattagg cgtagtagta gaaactccaa
     7261 ccttgaatcg ttccagtcca tattgtgcag tgtaacttct ggcgtaaaac ttaacgaatg
     7321 atcctgttcc taaaaacaca cgcgggctaa tcatccagtc attattagga ggtgtagtag
     7381 cagcaaaact ggcaggcatt ttagaaccgc tgtgagcagt taatgtagtt aaaggaggaa
     7441 ctgttgctga aggattaaag atgatatatg ccatagcatc tccgcttccg ggaaaatcaa
     7501 catcctgaat gccataagta tcagcaagat caacatcaac cgtagtccaa ggagcaaaag
     7561 tagtaacgaa attagcatag ctctcaaaat catctgtaaa gaatgcaggt gagggctgat
     7621 aattgatagt tacacttaca gttgcaggaa ctgattcccc tgttgtataa agtgcagtta
     7681 caccataatt ataagttccg ttggcaagat caagatcagt ataatcagta atggtaggat
     7741 tatttatagt agaaattaat gttccattac gatagacctt atagcccaaa agggcacggt
     7801 cattgttttt tgtctcttgt ctaatagtgg gaaccgaaac accctttact ctttcaatag
     7861 ttccaggaac ggaagctaca taaggatagc tttttgctgt ggcaggagtg ccgataaaga
     7921 catcatcaac caagaagata aaggcatcat tggaaacaca ttgaatacca atatagacat
     7981 tttgtcctgc atagctgctt aaatcataag tatattctgt ccaggtaacc ggtgcctgta
     8041 tatagcttgt tccgctaata atagtgaagt ttgcaggtgt tgttccagta gttgaaacac
     8101 cgactttaaa gcgttccaaa ccatattgat cggtataact tcttgcccaa aattttagca
     8161 cattgcctgc ggttatagaa agctgcggag taatcatcca atcattattc ggaggaacag
     8221 tagaagcaaa acaagcagcc attttattac cggtatgagc agttaaatca gttatggcag
     8281 gagtagtagc actcggatta aaaataatat atgcttgtgc cgcataagca ttagtccagg
     8341 aaatgccttg cattccataa gttgtgcttt ggtcaacatc cactaaagtc cacggagcaa
     8401 aactgattga gaaatcagtg taggtttcaa agccatcact aaaacctgtt ggaggaggaa
     8461 ctacaccggg ggcatcccaa tccagaaaga cattagtatt gttctgcact gtagcagtta
     8521 aattcagagg gggatcaagt ggagcaacaa tttctacact tacaggaccc gcaggaactg
     8581 attcaccagt agtataataa gcagtaactg tatagctgta agtgccaact tctaagccca
     8641 aatcatcata agtaagagta gcaggattac taatagtgtt aattaaagag ccatcccgat
     8701 aaaccttata gccaagaagg tctcttgtgc caaaagagga aacaggtgcc tgccaagcta
     8761 aatggacatt gtaataattt tgaatggtag cagtaagatt ttgaggtgga tttatctgtt
     8821 ctactatatc ggatacgctg aaagtatcta tatagagata gaatctatcg gcatcactat
     8881 gaccatgaaa acctatgtaa taagttcctc cggttactgc aggcataata atttctgctg
     8941 cttcataagc agtattgatg atgttcaaat tttcccaaag gagatttgtc atacttgcca
     9001 ctgcaggagt tgttccccaa tatacagaaa gcttttctgg ataagtggca cttgaactac
     9061 agtagtaaaa ttttattttg taattatgag tattggtaaa gacaagggga ggagtaaata
     9121 accagtcatc cattgctaat gtagaattat aacgcacacg catagaattg ggagatgttt
     9181 gactattgcc attataggac tgccaggtat aggtatcatt attggcatta ataactgtcc
     9241 aacctaaagc taaatcagga ggagtaactg aatcaaagtt ttgctcgtag ggataagtag
     9301 taactgtggg atcagctaaa acagtaaaac tccagacagg gcagttaatg gcatccccaa
     9361 attgattata gggaacaatt ttccagtaat aggtagtgct gtatgttaaa tcattaacag
     9421 gatcatatac agttactgtc tgtgtggttc cattaactat attggtagga ggattatccg
     9481 tgccaaaata gaccttataa ccatcaacaa ttccacctcc ggaattccag ctgaggtttg
     9541 tagctatggg cacattaata gctccattgg caggatgagg attttgagcc gctaaaggtg
     9601 gttctccagt tatgggagga gcgaaagtaa aggttaatcc attagcagga aatactgtag
     9661 cgcttaaagt gcaggtgctg ttattagcgg ttccggctgt tgttgcagac cagtccgtag
     9721 tagttaaacg gttattgaag tcattattgg aatcacctct taagccaacc tgcacagtgg
     9781 cagaagtagc agcagttaca gcagtaaagg gtccgtaaac aaagaccact ttgttaccgt
     9841 tttcctgaag ttgaatttga aaagtaaaat catcattagc tgtggcagta ggagttcgcc
     9901 tccaattttt ccattgtatg gtaaaaacac gattgggtgc agtgccactg cttaaagaca
     9961 taagagttcc atctgctctg gatttgagat cgcgagcaag aacagctatc acattattgg
    10021 tggaagtatt agaactgatc ggaacattac ttgttactac agtatccccc atagcgataa
    10081 aaccgttgga agcgatgctg attgttgtat aatcaacacc gttataggtg aaagtaaagc
    10141 ccagcggaat ggcacagaaa cattcattgt cattagcact ggtgccgtga attgttcctc
    10201 cactgatttc agtaaaagtt cctaatgttg aagcaaaaga atattcgctt accgtagaaa
    10261 atgcagcaat cgctgttact gaaaatatca gtagaagaag gagtttgtaa atttgtgctt
    10321 tcatagcatc tccttttctt gttaagtaac ttaatattat agacacctaa tcttgtctgt
    10381 tccataataa tttctatgtg gaaaacaaac tttttttacc tgataattat aactgcaata
    10441 ctgtcaagaa aaacttactt ttgttttgtt ttaactccac cctgcttgtt ttgacttaca
    10501 attttgccac agccatcaca gaaaccacaa cctgatgctt gttttgactt acaattttca
    10561 cttgccttcc agaaaaccac aaccctcaaa ttacaaacac tggcaaccaa attattactg
    10621 gtctcctcat ttcaaaattg tgagttaaaa caagaagcaa gcacccttac aaccgagcta
    10681 caacaaaccc acgaattcaa cggtcacgaa aaaacaaaaa agcaaactgg caacagcaat
    10741 acactatttc ttcaaacacc ggcagcgtga aagaaagtat tttatcgctc cagaaatgta
    10801 gggtggaatt gttcggaatt tgtggcttcg ggggatactt ttgttttaac tctaaaccga
    10861 gcttcgccaa cccctcaaat tccacaatcg tgaaagaaag tattttttcg ctccagaaat
    10921 gtggggtgga attgttcaga aatagtgact tcggggcgaa tttttgtttt aactctaaac
    10981 cgagcttcag ctgtgaaagt tgaaggcgac gaataacatt tcctgctgcc tcaaaataga
    11041 gttgcaaaca caaaagagtc ggtttagagt caaaacttca ctacctcacc atttctgtac
    11101 ctcgccctct ctctatctca ctaaatttat tcttttcgcg ataaacgaaa ataaagatga
    11161 tagttatgca aaacaacaac cagaaaaagt aggtctgcaa taagcattgc aatattaacg
    11221 gttaacggtt cccaggcagt aaaat
//
LOCUS       DAOPFL010000053        11143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_7096_length_11143_cov_90.248557, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000053 DAOPFL010000000
VERSION     DAOPFL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11143
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7096_length_11143_cov_90.248557"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PLG69_05000"
     CDS             <1..1223
                     /locus_tag="PLG69_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPI42902.1"
                     /translation="NLIIEKKLPWQTVFEVFGLSLPYMLALSIPMAVLVATILAFGRM
                     SVDREITAIKSSGINVYSLIGPLVLAALLLTGLMVYFNHWFLPDTNHKLKNLMLKIAY
                     YKPMTIIKEKEFTNFMDYTIYARETTDSLLTDVLIYDRSQSHFPRTIFAKSGNVIQKD
                     NGNSLQIILNDGEMHERNEKEVGKYQKTTFSRYIINVRNLGSNMEMFETGYRSDREMT
                     YNQLITAIAEHKKELQTKQQEIEELNKRIELLQKSPQNFTRNMELRRLQAMQKMVIDR
                     TQELKELINSLLVEYHKKFSMSFAIIIFVLIGIPLGLMTRTSGIGMSFSVSSIIFLVY
                     YIALNGGEQLADKGFMPPFLAMWLANIIFLILAVLLIIGSIKEKRLLDMPTLMWKIKH
                     YRNRKAPVPDEIVH"
     gene            1226..2314
                     /locus_tag="PLG69_05005"
     CDS             1226..2314
                     /locus_tag="PLG69_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPI42903.1"
                     /translation="MRILDKYIIREFLRTYLIIFLSFVAIFVVIDVIDNLPRLIRNGA
                     TAQQATIYYLMRLPYLLVLTSPVTVLLTGLFMMSALSKHNESIAIRAAGISIKRAMLP
                     LFGIGLIISIAVAVFGEYVVPWAETTKNYVYNVQIKGQQPDDQMLKARIHYQGKANDF
                     YYFGFFDGYKNNLKIIDLTRIDFKTKQITEHITATSADWNGSRWILKDCDIRRFANGK
                     QVFMAHYPQTDLVILDVQPQDFIRISQKTLSLNFWQLKEYIGRMKKLGDNPAREIVDL
                     NMKISFPLTNLIVIFFFIPIATSNIRSKGRGWIIMLGLVVCFIYLIVVRVCQSLGYNS
                     IINPFWAAWFPNIFFTLLGLGFLHKAEI"
     gene            2673..4751
                     /locus_tag="PLG69_05010"
     CDS             2673..4751
                     /locus_tag="PLG69_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPI42904.1"
                     /translation="MADNNRIAIHPILPIPEREEVFFYWNGQKLKAKSGEMIASALIA
                     NGISIFGHHHKDGSAQGIFCANGQCSQCSVIANGVAVKSCMTAVSENMIVQSLEGLPI
                     LPAEDAPLNFEPLEYIKTDVLIIGGGPAGLSAAIELGKQNIQTLLIDDKNALGGKLVL
                     QTHKFFGSQEDSSAGTRGIDISKNLSEELAKFGSVEIWLNSTAVFVFSDKKVGVLKDG
                     VYKIVEPKRILNTAGAREKFLRFPGNNLARIYGAGAFQTLVNRDLVRPTNRLFIIGGG
                     NVGLIAGYHSLQAGIEVVGLAEAMPVCGGYKVHSDKLKRFGVPIYTSHSILCANGKES
                     IESITIAEIDKNFQPIPKTEKTFACDTILIAVGLNPLNEFTWEAMDAGIPVEAAGDAL
                     EIAEASSAMFNGKIAGVKIAADLKGNKDNPIPKEWYAKAEELKSPPGITKSYQTPEKE
                     EGVFPVLHCLQEIPCNPCTTVCPTNSIKTEDGSLMAVPVYQGSCIGCGKCLLICPGLA
                     ITLVDYRKDKENPTVSIVYEVTNYPVQIGDKKILNDIDANELGEYAITAVQDFPKQHT
                     QIVKFQVPKAIAKKVAGFRIQDASVSAPIDKPIIFEKTSDEAMICLCERVSVGQVQAL
                     IKKGITDLNLIKAITRAGMGPCGSKTCEVLIKGLLREEGIPDKEVVANTKRPLFIEIP
                     LAKFPDGSVK"
     gene            4748..5902
                     /locus_tag="PLG69_05015"
     CDS             4748..5902
                     /locus_tag="PLG69_05015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPI42905.1"
                     /translation="MKNIYDIIVIGAGSIGLPTAYNLAKAGMKVLVLEAEHSPGQGNN
                     KKAIGGVRATHSDFGKINVCLRSIEILKNWQEETGEDIGWLSNGYSYPAYNEKDAQEL
                     QDLMKIQHSFGLNIKWLSPDEYSEIVPGILQEGLQGSTYSPEDGSCSPLLVSAAYFEH
                     SQKAGVEFHFQERVTGFDIRAGKINKVITNKGIYSCAKVVNAAGSYAREIGKLANLDL
                     PVFPDNHEAGITEPVERFFGPMVIDMRLAPGSANFYFYQNIEGQIVFCITPEPPIEGI
                     DNRSTSVFLPMCSKRMLTIYPRLRNLKVRRTWRGQYPMTPDGFPLVGIMQEVENLVNA
                     VGMCGQGFMLGPGMGELVTRICLENPTDKDLKILESFDPYRQFTSQEAFK"
     gene            complement(6050..6697)
                     /locus_tag="PLG69_05020"
     CDS             complement(6050..6697)
                     /locus_tag="PLG69_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPI42906.1"
                     /translation="MKPFLLFDFDGTIADSLQLGLKIANNLAPQFGYPLFTENDIQHF
                     RSLTWHKIAREMQIPFYKIPKIVTIAFKEYKRLISELEPCAGIVEMIKELALKEIPMA
                     LLSSNTVENVHLFLTNHKIDSFLWIEGTSGILNKAKDIKKRLKKHKISTNEVIYIGDE
                     IRDIEAAHNCGLKVIAVTWGFHTAEFLASYKPDYLVNEPKEIVEIVNRLTVKSEK"
     gene            complement(6694..7602)
                     /gene="rsmI"
                     /locus_tag="PLG69_05025"
     CDS             complement(6694..7602)
                     /gene="rsmI"
                     /locus_tag="PLG69_05025"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPI42907.1"
                     /translation="MPDFYLPALSFFLFIMPSGNIYLIPVPIGNLGDITLRALELLKK
                     AELIACEDTRVTAFLLQQYKIPVPKLISFHKFNEKQREEYFFEYLQTGKDLMIVSDAG
                     SPAISDPAEHIVKEAIAKGIKVFALPGASAILPALSVSGFTTRQFQFVGFLPQQPKNR
                     RENLEQIAEYPYTTVIYEAPHRIWKLLNELYAVCGNRKICICREISKLHEEHIHTTLE
                     EINKNPSLTIKGEFCLILDGQKIKVEEEKEQEELNEDDLIDYINSALDLGLKTKDIRD
                     MVCLKSSLSLKDAYQLVISVKKERGK"
     gene            complement(7617..8807)
                     /gene="kbl"
                     /locus_tag="PLG69_05030"
     CDS             complement(7617..8807)
                     /gene="kbl"
                     /locus_tag="PLG69_05030"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPI42908.1"
                     /translation="MAYGKIKSDLQELFKELKEQGLYKEERILTTPQGARIGVQGGKE
                     ALNFCANNYLGLGNNPEVIKAAQDIMNDWGYGCNSVRFICGTQAIHKQLEEAMSKFLG
                     MEDTILYAACFDANGGLFEPLLGEDSAIISDELNHASIIDGVRLCKAKRLRYKHSNME
                     ELENALKESQDCTYRIICTDGVFSMDGDMAKLDEICNLADKYDSLVMVDDSHATGYIG
                     KNGRGTPEYFGVTDRIDIITTTFGKALGGANGGCTSGRKEIIELLRQRSRPYLFSNTL
                     APSIVGATLKVLEMLSHSNELRDKVWENASYFRKEMVSAGFDIVPGNTAIVPIMLYEA
                     PLAIKMADMLLEEGVYVIGFVYPVVPKGKARIRVQLSAAHTKEDLDFTISAFKKIGRE
                     LGLI"
     gene            8996..9712
                     /locus_tag="PLG69_05035"
     CDS             8996..9712
                     /locus_tag="PLG69_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42909.1"
                     /translation="MQKIIIILCTLVCLSFGAEAISIYEIQFTASAGSDGTFPSPYLG
                     KTVSTEGIVTAMDFKEGGYFIGEKLNGPWRGILILDRNPKVKKGDFIRVSGIVSEFYG
                     MTCLQDISQTKVISSNNPLPQPVLLTTGQLSNAGEAEAYESVYVRFLNVTSAGNKSLK
                     NRFTVTDGSGTCLVLNNQFGIERNHKIALNAQYSSLTGIVIYGYSEFALCPISTSDYV
                     IQQPMSVQNRSWGRIKSIYR"
     gene            9738..10106
                     /locus_tag="PLG69_05040"
     CDS             9738..10106
                     /locus_tag="PLG69_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42910.1"
                     /translation="MKKFFKKILILGGTLAAAHFGFKAYKKISGTAKLSKSLPEFLNN
                     VYGEMPQVNVNHTLNSTRIKLFFSQEILDKHTDIETTIREYIDDFYPEIAKTSVDISI
                     MPATEETEDIPVEEETEEKE"
     gene            complement(10814..>11143)
                     /locus_tag="PLG69_05045"
     CDS             complement(10814..>11143)
                     /locus_tag="PLG69_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPI42911.1"
                     /translation="AEMKHGPIALIDKNMPVIAIATKDPLYDKIYSNLQEVRARKARL
                     ITIATEGDTELSKISENVIYIPDTLTNLQPLLTVIPLQLLAYNVADLKGLNVDQPRNL
                     AKSVTVE"
BASE COUNT         3405 a         2053 c         2156 g         3529 t
ORIGIN      
        1 tgaatctgat aattgaaaag aaactgccct ggcaaacggt ttttgaggtc tttggtcttt
       61 ctctgcctta tatgcttgct ctttccattc caatggctgt gctggtagct acaattcttg
      121 cttttggcag aatgagtgtg gatagagaaa ttacagctat taaatccagc ggtataaatg
      181 tttattcttt aatcggacct ttggtgctcg ctgccttgct gttaaccgga ttgatggtgt
      241 attttaacca ttggtttttg cccgatacta accataaatt gaaaaacctg atgctgaaaa
      301 ttgcttacta caagccaatg acaataatca aagaaaaaga atttaccaat tttatggatt
      361 atacaatata tgccagagaa actactgata gcttgctgac agatgtttta atctatgatc
      421 gtagtcagtc acattttcca cgcaccattt ttgctaaaag cggtaatgtg attcaaaaag
      481 ataatggcaa ttctctccaa ataattttga atgacggtga aatgcatgaa cgcaacgaaa
      541 aagaagtcgg taaataccag aaaactacct tcagtcgtta tataattaat gtacgaaact
      601 tgggcagtaa tatggaaatg tttgaaactg gctatagaag tgaccgtgaa atgacttata
      661 atcaattgat tacagctatt gctgaacaca aaaaagaact gcagacaaaa cagcaggaaa
      721 ttgaagaact aaataaacgc attgaattac tccaaaaaag ccctcagaat tttacccgca
      781 atatggaact gcgtcgttta caggcaatgc aaaaaatggt aattgaccgc acccaggagc
      841 tgaaagaatt gatcaactct ttactagtgg aatatcataa aaagttttct atgtcctttg
      901 ctatcattat ctttgtcctg attggaattc ccctgggttt gatgaccaga acaagcggaa
      961 ttggaatgtc tttttccgtt tcttctataa ttttccttgt ctattatatt gctcttaatg
     1021 gcggtgaaca acttgccgat aaaggattta tgcctccatt tttagctatg tggctggcta
     1081 acattatatt tttaatttta gccgttctgc ttataatagg ttccataaaa gagaaacggc
     1141 ttctggatat gcctactctg atgtggaaaa ttaaacatta tcggaataga aaagctcctg
     1201 ttcccgatga gattgtgcac taaaaatgag aattcttgat aaatatatca tccgtgaatt
     1261 tctgcgcacc tacctaatca tctttctttc ctttgtagct atctttgtgg ttattgatgt
     1321 aattgacaat cttccacggt taattcgcaa tggagctacc gctcaacagg caactatata
     1381 ctatttgatg aggttacctt atctgcttgt gttaacttca ccggttacgg ttctgttaac
     1441 cggtttgttt atgatgagcg ctttatccaa acataatgaa agtatcgcta ttcgtgctgc
     1501 cggaattagt ataaaaagag ctatgctacc tctttttgga attgggctta taatcagtat
     1561 tgcagttgcc gtatttggag aatatgttgt tccttgggct gaaactacta aaaattatgt
     1621 ttataatgtc caaatcaaag gtcagcaacc ggatgaccaa atgctgaaag cccgaatcca
     1681 ctatcagggt aaagctaacg atttttacta tttcggtttc tttgatggct ataaaaacaa
     1741 cctaaaaatt attgacctta cccgtattga ttttaaaact aaacagataa ctgaacatat
     1801 aactgctacc tctgcggact ggaatggttc tcgctggatt ttaaaggatt gtgatattag
     1861 aagatttgct aatggtaagc aggtctttat ggctcattat ccgcaaactg atttagttat
     1921 tctggatgtt cagccccagg attttatccg catttctcaa aaaaccctct ctctcaattt
     1981 ttggcagtta aaagaatata tcggcagaat gaaaaaactg ggagataatc cagcaagaga
     2041 aattgtggat ttaaatatga aaatttcctt tccgcttacc aatctcatag ttatcttttt
     2101 cttcatcccg attgccactt ccaatattcg ctccaaagga cgcggctgga taattatgct
     2161 cggtttggtt gtctgtttta tctacttaat tgtggtaagg gtttgccaaa gtttgggcta
     2221 caacagcatt atcaatccct tctgggctgc ctggtttcct aacatcttct ttactctttt
     2281 gggcttgggc ttcctccata aagccgaaat ttagtgtagt tggagctcgc cgaagctccg
     2341 catctttaaa ccaggttttg aatggatgta acttttttag cggaggattg gcatcctcct
     2401 tctgcacaag catcacaata atgacattct gcaggtctgg ctttatttgc accagtaaaa
     2461 ttgcataaga aacaaaatca taatggtgta aataactcct aactttcaat aattcaatta
     2521 ccctgttttc attaactcca ttgctacaat tactgcttga caaacagcca agtacaaaaa
     2581 ttgaggatag aataaataat agtaatgtga ggttaggtgc taaaaccata aatgaaagaa
     2641 cctaacttcc ttatataagg agttttactt tgttggcaga taacaatcgt attgccattc
     2701 atcctatatt gccgattccg gaaagagaag aggtcttttt ttactggaat ggacaaaaac
     2761 taaaggcaaa aagcggagaa atgattgcct cagctcttat tgccaatggt atatctattt
     2821 ttggtcatca tcataaagat ggcagtgcac aaggtatatt ttgtgctaat ggtcaatgtt
     2881 ctcaatgtag tgtaatagct aatggcgtag ctgtaaagtc ctgtatgaca gctgttagtg
     2941 aaaatatgat tgtgcaatcg ctggaagggt tacctatcct tcctgcagaa gatgcaccct
     3001 taaactttga acctctggaa tatattaaaa ccgatgttct tattattggt ggaggtcctg
     3061 cagggctttc tgctgctatt gaactgggca aacaaaatat ccaaacattg ttgattgacg
     3121 ataaaaatgc tttaggggga aaacttgttc tgcaaacgca taagttcttt ggttcgcagg
     3181 aagatagcag tgccgggacc agaggtattg atataagcaa aaatctctct gaggaacttg
     3241 ccaaattcgg tagtgtagaa atttggctaa atagtacagc tgtcttcgtg ttcagtgata
     3301 aaaaagtagg tgtcttgaaa gacggtgtct ataaaatagt tgaacccaag cgtattttaa
     3361 acactgcagg agcaagagaa aaattcttaa gatttccggg gaacaattta gccagaattt
     3421 atggagcagg agcttttcag accttggtaa atagagacct tgtgcgtcct accaatcgtc
     3481 tttttattat tggtggtggc aatgttggct taattgcagg ttaccattca ttgcaagcag
     3541 ggattgaagt tgtaggtttg gcagaagcaa tgcctgtttg cgggggctat aaagttcatt
     3601 ccgataaact gaaacgcttt ggagtgccga tttatacttc gcattctata ctttgtgcta
     3661 atggtaaaga atcgattgaa agcatcacta ttgccgaaat tgataaaaac tttcaaccta
     3721 tacctaaaac ggaaaagacc ttcgcctgtg atactatttt aatagctgtg ggcttaaatc
     3781 ccttaaatga attcacttgg gaagcaatgg atgcaggaat tcctgtggaa gctgcaggtg
     3841 atgctttaga aattgcagaa gcaagttcag caatgtttaa cggtaaaatt gccggagtga
     3901 aaatcgctgc agacctgaaa ggaaataaag ataatcctat tccgaaggag tggtatgcca
     3961 aagcagaaga gctaaagtcg cctccgggaa ttacaaaaag ctatcagacc ccggaaaagg
     4021 aagaaggtgt attccccgtt ttgcattgct tgcaggaaat tccctgtaat ccctgcacaa
     4081 ctgtttgtcc tacaaatagt atcaaaactg aggatggttc actgatggct gttcctgttt
     4141 atcaaggttc ttgtattggc tgtggaaaat gccttttgat ttgtcccggt ttagccataa
     4201 ccttagtgga ctaccgaaaa gataaagaaa atcccacagt cagcattgtc tacgaagtaa
     4261 ccaactatcc agtccaaata ggggataaaa aaatcctgaa cgatatagat gccaacgaat
     4321 taggagaata tgccattacc gctgttcagg attttcccaa acagcatacc caaatagtaa
     4381 aattccaggt gcctaaggca attgctaaaa aagtagccgg ttttagaatt caggatgcaa
     4441 gtgtctctgc cccaatagat aaaccaatta tttttgaaaa gacatccgat gaggcaatga
     4501 tttgcttatg tgaaagagtc agtgtagggc aagtgcaagc ccttattaaa aaaggcatta
     4561 cggacttaaa tctgattaaa gcgattacca gagcaggaat gggaccttgt ggttccaaaa
     4621 cctgtgaggt tttaataaag ggtttgcttc gtgaagaagg cattccggat aaagaggttg
     4681 ttgccaacac caaaagacct ctttttatag aaatcccttt agcaaaattt cctgatggga
     4741 gcgtaaaatg aagaatatct atgacataat tgtaattgga gctggttcaa tcggattacc
     4801 gaccgcttat aatttagcta aagcgggaat gaaggtttta gttctggaag cggaacatag
     4861 tcccggacag ggaaataaca aaaaagccat cggtggagta cgagctacgc attccgattt
     4921 tggtaaaatc aatgtctgcc ttcgttctat tgaaattttg aagaactggc aggaagaaac
     4981 gggtgaagat atcggctggc tttccaatgg ctatagttat cctgcttata atgaaaaaga
     5041 tgcccaagaa ttgcaagacc tgatgaagat acaacatagt tttggactta atattaaatg
     5101 gcttagccct gatgaatata gtgaaatcgt tcctggtatt ttgcaagagg gcttgcaggg
     5161 ttcaacttat tctccggaag atggttcttg ttcaccatta cttgtttctg ctgcctattt
     5221 tgaacattcc caaaaagcag gagtggaatt ccattttcag gaacgcgtta ccggctttga
     5281 cattagagcg ggcaaaataa ataaggttat tactaataag ggtatttatt cctgtgctaa
     5341 agtggttaat gctgctggaa gttatgcacg cgaaattggc aaactggcta atttggattt
     5401 acccgttttt ccggataatc atgaagcagg gattaccgaa ccggtagaaa ggttttttgg
     5461 tcctatggtt attgatatgc gtttagcacc aggaagtgcc aatttctatt tttaccagaa
     5521 tattgaaggg cagattgtat tttgtataac tcccgaacca ccgattgagg gaattgataa
     5581 tcgttccact tctgtctttt taccaatgtg cagtaaaaga atgctgacaa tttatccgcg
     5641 tttacgcaat ttgaaagtgc gcagaacctg gagaggacag tatcctatga ctccggatgg
     5701 ctttccttta gtaggaatta tgcaggaggt ggaaaacctt gttaatgcag taggaatgtg
     5761 tggtcagggc tttatgcttg gtcccggaat gggagaactg gtaacccgta tttgtttgga
     5821 aaaccctaca gataaggatt tgaagatatt ggagagtttt gatccttatc ggcagtttac
     5881 gagtcaggaa gcgtttaaat agatttctat tacaaagaga aaagtgaaag tttagccacc
     5941 gaagtcaccg aaaaggaatt tttgcagaag aaagaatagg gagaaagatt attgaataat
     6001 ttcggtgttc tcagtaactt aggtgtgctc ggtgtaaaat aattcctttt cacttttcac
     6061 ttttcaccgt taacctgttc actatttcta ctatttcttt cggttcattt acaaggtagt
     6121 cgggtttata gcttgccaaa aattcagctg tatgaaatcc ccaggtaact gcaatgactt
     6181 ttaatccgca attatgggct gcttcaatat ctctgatttc atctcctata tagattacct
     6241 cattagtaga tattttatgt ttttttaagc gttttttaat atcttttgcc ttattgagga
     6301 tgccggaagt tccttcaatc cacaaaaagg aatcaatttt atgattagtt agaaagagat
     6361 ggacattttc cacggtatta gaacttaaga gagccatagg tatctctttt aaagcaagtt
     6421 cttttatcat ttcaacaatt cctgcacaag gttcaagttc agaaataagg cgtttgtatt
     6481 ctttaaacgc aattgtgacg attttaggta ttttgtaaaa aggaatttgc atttccctgg
     6541 ctattttatg ccaggttaaa gagcggaagt gctgaatatc attttccgta aagagcgggt
     6601 aaccaaattg aggagcaaga ttattggcga tttttaaccc cagctgaaga gaatctgcta
     6661 ttgtgccatc aaaatcaaac agcagaaagg gtttcatttt cccctttcct ttttaacact
     6721 aattaccagc tggtatgcat cttttaaaga aagggaagat tttaaacaaa ccatatctct
     6781 aatatctttt gtctttagtc ccaaatccag ggcgctattt atataatcaa taagatcgtc
     6841 ttcatttaat tcttcttgtt ctttttcctc ttcaacttta attttttgac catctaaaat
     6901 taggcaaaat tcacctttta tagtgaggga cgggttttta ttaatttctt ctaaagtagt
     6961 atggatatgt tcttcgtgaa gtttgcttat ttctctgcaa atacatattt tccggttacc
     7021 gcaaaccgca tagagttcat ttaacaattt ccaaatacga tgaggtgctt catagataac
     7081 tgtagtatag gggtattcag caatttgttc cagattttcc cgtctgtttt taggttgctg
     7141 aggtaaaaaa cctacaaatt gaaactgcct tgtagtaaag ccggaaacac ttaaagcagg
     7201 taaaattgca ctggctccag gcaaagcaaa aacctttatt cctttagcaa ttgcttcctt
     7261 tacaatatgt tccgcggggt cactaatggc aggacttcct gcatcagaaa caatcattaa
     7321 atcctttcca gtttgcaagt attcaaaaaa gtattcttct cgctgttttt cattgaattt
     7381 atgaaagctt atcagtttcg gaacgggaat tttgtattgt tgcaagagga aagctgtaac
     7441 tctggtatct tcacaggcaa taagttccgc ttttttcagc agttccagag ctcgtaaggt
     7501 tatatctcct aaatttccta taggcacagg aataaggtaa atatttccgg agggcataat
     7561 aaataaaaaa aagctaagtg ccggtaagta gaaatccggc acttataaat tagctgttaa
     7621 attaaaccga gttctctgcc aattttctta aaggcagaaa tcgtgaaatc caaatcttct
     7681 ttagtgtggg cagcagaaag ctgaacccgt atccgtgctt tgcctttggg gacaacagga
     7741 taaacaaagc caattacata aactccttct tctaaaagca tatccgccat ctttatagct
     7801 aaaggtgctt cataaagcat aataggaacg attgccgtat ttcctggaac tatatcaaaa
     7861 ccagcagaaa ccatttcttt ccgaaaataa cttgcatttt cccaaacctt atctctcagt
     7921 tcattagaat gagaaagcat ttcaagaacc tttaaggtgg cacctactat agaaggagct
     7981 aaagtattag aaaacaaata aggtcggctt ctttggcgca aaagttcaat tatttctttg
     8041 cgtccggaag tgcaaccacc atttgctcca cctaatgctt taccgaatgt ggtagttata
     8101 atatcaattc tatcggtaac gccaaaatat tccggagttc ctcttccgtt tttgccaata
     8161 taacccgtgg catgagaatc atcaaccatt accaaagaat cgtatttatc ggcaagattg
     8221 caaatttcat ccaactttgc catatctcca tccatactga aaacaccgtc cgtacaaata
     8281 atacgataag tgcaatcctg gctttccttt aaggcatttt ccagctcttc catattgcta
     8341 tgtttatagc gcaagcgttt tgccttgcaa agacgaacgc catcaataat tgaagcatga
     8401 tttaattcgt cggaaataat tgcgctatct tcacccaaaa ggggttcaaa caaaccacca
     8461 ttggcatcaa aacaggcagc ataaagaatg gtatcttcca ttcctaaaaa cttggacatt
     8521 gcttcttcca gttgtttatg aattgcctgt gtcccgcaaa taaaacgcac ggaattacat
     8581 ccataacccc aatcattcat aatatcttgt gcagctttga ttacttcagg attattacct
     8641 aacccgagat aattattagc acaaaagttt aaagcttctt taccgccttg aacaccaatt
     8701 cttgcacctt ggggagtggt taaaatgcgt tcttctttgt aaagtccttg ttctttgagc
     8761 tctttaaaga gttcttgcaa atcggattta atttttccgt aagccatttg attcctcact
     8821 ttataataag tttttcttta tttaacccaa attataagtt cttttatttc gtcaatggaa
     8881 tttatttttc tttcccaact cacaattaca ctatctcatc aattcgcttt ctaacttaaa
     8941 tgattttact tgacaggata ttgcatttaa gtattatgta attatagagg taataatgca
     9001 aaagataatc attattctat gcactctggt ttgcctcagt tttggggctg aagccatttc
     9061 tatatatgaa attcagttta ctgcttcagc aggcagtgac ggaacttttc cctctccata
     9121 tttaggtaaa accgtatcca cagaaggaat tgttacagct atggatttca aggaaggcgg
     9181 ttattttatc ggcgaaaagt taaatggtcc ctggcgggga attcttatac tggatagaaa
     9241 tccaaaagtt aaaaagggcg attttataag agtttccggc atcgtttccg aattttacgg
     9301 aatgacttgt ttacaagata tttctcaaac taaggtcatc agcagtaata atcctcttcc
     9361 gcaaccggtt cttttaacta caggtcagct atctaatgcg ggagaagcag aggcatacga
     9421 aagtgtttat gtccgctttt tgaatgttac atctgcaggc aacaaatctc ttaaaaatcg
     9481 tttcacagta actgatggca gtggcacttg tcttgttttg aataatcaat ttggcataga
     9541 gcgcaatcat aaaattgcct taaatgctca atattcgtct ctcacaggaa ttgtaattta
     9601 cggctatagc gaatttgccc tttgtcccat ttccacatcg gactatgtta tccagcagcc
     9661 aatgtctgtt cagaaccgtt cctggggtag aattaaatcc atctacagat aaaaaataaa
     9721 ctaatgaggt aataacaatg aaaaagtttt tcaaaaaaat cctgattctt ggtggcacac
     9781 ttgccgctgc tcattttggc tttaaggcat ataaaaaaat tagcggaact gctaaacttt
     9841 ccaaatcatt acctgaattt ttgaataatg tttatggcga aatgccacag gtaaatgtaa
     9901 atcacaccct aaattctacg cgcattaagc tcttcttcag tcaggaaatt ctggataagc
     9961 acaccgatat agaaactacg attcgtgaat atattgatga tttttatcct gaaattgcta
    10021 agacctctgt agatatcagt attatgcccg ctactgaaga aacagaagat attcctgttg
    10081 aagaagaaac tgaagaaaaa gaataagccc ttatttatcc ctgattattc ccaaaacccg
    10141 gtttctaacc aaaaccgggt ttttgtgtag ctggaggata ccattcctcc gcaaagaaaa
    10201 aaattgcggt gcttcagcga actccgacta cccggtaaaa ggtctgtaat ttattgatgt
    10261 ttttttgaga tttatccaat tcggaaatcc taaaagtttc gttttcagtt agtaaccatc
    10321 caagaaatta ttattgctcc ctacatcctc taataattat ataacatttc tgtaacgact
    10381 ccgtaacgag ataagggagg tgttacggaa gtgttacggc agtgttacgg ggtttacttg
    10441 gaacgaattg ataatcaata aattgcgaac atacaattga ggggaaattg ggggagaaaa
    10501 ttattccttc attttacgag gggattacgc cttcatcgct attattgtgt tgcccttcgt
    10561 acttttagca aagggcaaag agcgaagggc aaagggtttt ttggtcattt ttaaaaagag
    10621 gtagatggga acaccaaact tccaatgtta cttttctggt ttttaaggaa tcttaacctt
    10681 ctaaaccctt tcaactttct aaactttgta atcccttttt ccaacctgcg ttttaaataa
    10741 ttgtaaatcc gttataatcg gtaaaatccg tgttcttttc ttttatcttt gttatctgtg
    10801 aaatctgtga ggattattct acagtaacgc ttttggctaa gtttcggggt tgatcaacat
    10861 tcaaaccctt taaatcagct acattatatg ccagcaactg taaaggaata acggttaaaa
    10921 gcggttgcag attagttaag gtatcgggaa tataaattac attttcagat attttagata
    10981 gctctgtatc gccttcggta gcgatagtta tcaaccgtgc tttccgagcc cgaacttcct
    11041 gtaaattgga ataaatttta tcgtagaggg ggtcttttgt ggcaatagcg atcacaggca
    11101 tatttttatc tattaaggca ataggaccat gtttcatttc tgc
//
LOCUS       DAOPFL010000054        10958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_7285_length_10958_cov_113.213886, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000054 DAOPFL010000000
VERSION     DAOPFL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7285_length_10958_cov_113.213886"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2964
                     /locus_tag="PLG69_05050"
     CDS             <1..2964
                     /locus_tag="PLG69_05050"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPI42912.1"
                     /translation="LYHYTSNGIDPAGSWDLMCSDQNPPQHMTAFMKWKYGNWIATIP
                     TIFIGQQYTLNPLTSSTGNCYRIDSNNPNQYYVVEFRKKTGTFESSIPGSGMLIYRID
                     TTAGDGNASGPPDELYIYRPNGTTTVNGTINSANFSQETGRTSINNTTNPAPFLQDGS
                     EGNLNIYAIGSSAGTTMTFSLGVPTVDFSTNPYTESFDSSTFPPTGWYKQTISGSYGF
                     ERVTSGTHPTCSPQAGAGMICYKSYNALSSNSAILATLRLNCSEVQFYSYDFSFYMYR
                     DTGKSSNADRILIYLADTPDMSGTNTLLGTINRYTGFTPTVPSNGWYQYSFNLPLTTS
                     GYYYVVCQAISAKGNNMFLDSFSIKKTALPPSPAISPSPADLAENVSVSPIFSWANGG
                     GYPSHYLFYLGTNNPPTNLIDGLNLGNVLNYAYTGTLNYSTQYYWKVIPVSEGGSAQN
                     CSVWSFTTYADPRITTLPYRENFDIVTAPTLPFGWETYISSSNSSAFVKNFSSPTNYA
                     LSPPNCINFNNFTDTSAQLILITPEILTPINILRLKFYAKGSATGYSLQVGTMSSPNG
                     TFTSLTTVNLTNTMQQYSVPFSNYNGTDRFIAIRHNLGGSNRNIYLDNFYLEEELTND
                     LAISALQMNGLGVIGEPLQFSVSVLNNGIATQNSYSIQLLSNSAKTVLANLDVLEPLA
                     PGQTAVHNLTWIPETEQTLSVYAQVTLISDGYTGNNTSIPSPISIGSYIPKIGTTDTG
                     TKTNLLPLNFNQKNSISETIYLASELQMTAGIITAIAYHTNFVQDLSHQAVKIWMKNT
                     PESNLSSGWLSFDEYVPVFDGFVDFPAGSNTIIIILQIPFPYNGNNLAIRVNRPFDNN
                     NYNTSNHFYYTNSTSNPGRSRYLSNSSVIYDPQNPSALGSLSNFIPVTGFSVTSYIPA
                     VLAIPQLLIQKNQQGVLLNWNAVPGANLYRIYASSDPVVWSDIPYAECSQTSFLITNP
                     PDKMFFKIVAVYQAP"
     gene            3092..3167
                     /locus_tag="PLG69_05055"
     tRNA            3092..3167
                     /locus_tag="PLG69_05055"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3125..3127,aa:Phe,seq:gaa)
     gene            complement(3220..4386)
                     /locus_tag="PLG69_05060"
     CDS             complement(3220..4386)
                     /locus_tag="PLG69_05060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42913.1"
                     /translation="MKVLIIPSWYPYPQKPNTGRFFLDQAIALSRNSNYEYEILNWGQ
                     NEYQLQIRHPFVAVSKIVNYLTAKTRTHNITPNLKEIKIPHLTWTSRLFYGNIKALVK
                     KLDSLSRPDIIHSYVTFPAGFLAFRLAEKWKIPYIISEHSGPFPFPEYVIRNKVSKKI
                     TLPLHNASAVLAVSRFLQAQILSSCSVQSIVIPNLVDTEFYQPADKEEGKCFSFFSLS
                     ILTYAKGVMDLVDAFALAVQKGLDADLYLGGDGYLRSKIRQKITKYHLEHRLHLLGYL
                     NPQQALEQYQKCDCYILPSHIESFSLVLLEAMACGKPTLATDCGGPRDIVTPETGILI
                     PPKKPFLIAEAMLKMKENINNYSPARIREICITNYSPPVICKKITAIYQNILQK"
     gene            complement(4383..5657)
                     /locus_tag="PLG69_05065"
     CDS             complement(4383..5657)
                     /locus_tag="PLG69_05065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI42914.1"
                     /translation="MKNVLLIFYYFPPAGGSGVQRGLKFAKYLPEFGFKPVILCAYYR
                     FLKQPRDYTLLRELPDKVKIYRSFTLDINWFYKLLWGLKLNKIVNFLRRNVFIPDGEI
                     LWLPFAIHKLKKIMATNNINQVLVSVPPYSLIFLAKYLKKHYQIKVCLDFRDPWSFGI
                     GRKYLKPPDWVTAIENRWEKEIVTRADQVICVSSVMIEEFRHLYPYLDKNKFVCITNG
                     YDEKDFPVSFPPIRNAKFTIIYTGSFYDELQPDILWQAILELIQEGCLNPQKIAVEIY
                     GRNFRNFVLGKYITDPILNQIVHFHGYINHRNSIQILRAADVLLLYLGSGEAQRAIVT
                     AKVFEYLRSGKPILAIIDSSGAAADILKPANTAFIADSSSIHSIKETLGNLYRLWEED
                     KLQTEPNWDYIQQFERKALTAKLAEVFSALEQ"
     gene            complement(5654..6406)
                     /locus_tag="PLG69_05070"
     CDS             complement(5654..6406)
                     /locus_tag="PLG69_05070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPI42915.1"
                     /translation="MNYMAEATWENYDPEKQADLRIAKLLALLPSDVQTILDAGCGNG
                     IITNKLIAKYQVTGLDISPAALKYLQCPSVQASVTEIPFPDRSFDAVICNEVLEHLDN
                     VSLQKAFPELKRVTKKYIIISVPHKEQLEKLYYRCANCGFIEHPYGHLQSFSEETLST
                     YLAPEFYLKKREVFGPLNRDFIPLLLNIKQHWLKQWFNPASGLKCSRCQQEKFEINKS
                     LLTKGINLLNRLLVHPRPYWYMGLFIRKTQEL"
     gene            complement(6403..7866)
                     /locus_tag="PLG69_05075"
     CDS             complement(6403..7866)
                     /locus_tag="PLG69_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPI42916.1"
                     /translation="MSELPKQAGILSATEFFRLAIKAVIGIALAHLLTPSELGSYRQL
                     FLIYTTFSTLLLLGIPQSMLYFLPKAESPEKQNALITRCLNLISLLGLIFALCLLIFR
                     KPIATLFNNPALEKLLILYALYPIFLFVTQFFSSVMLGLKQPFKAATFTIFAIIADFI
                     LIVGSAFLTRNLTIIVCGVISSAFLQWLYARISLAKFKAKETRFDLGSFKTLMSYSLP
                     LGLSSIIGMMSIQLDKFMVSAFFSPEKFAVFSIGATEFPIVGILANSINSVLLPHLSS
                     SNPEKMGILYSGAVRKNTILVFPLMALCYIFAEPAITLIYGKLYTESAIYFRIYLFLL
                     PLRIATYGILFQAFGKTRFVMYNSLFLLISNAILNYLLIIKFGMKGAAFATVIVTWLS
                     VIIYLIQIAKVLKLNLMAFFPLVKIAKNAILTVICAIICVLIIKLGNSTIPFLLTGGV
                     VFTIAYFFLGRKIGVILDYDVQLAREMLTDFFSRFKK"
     gene            complement(7859..9307)
                     /locus_tag="PLG69_05080"
     CDS             complement(7859..9307)
                     /locus_tag="PLG69_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPI42917.1"
                     /translation="MKKGFFILFTILFVKINAEIRSVWVLPWDITTEKSIDEVIATAV
                     SCNQNELLVEVRYRADALFDTSKGAYLYPNPEPKSYILEDASFDPLAYILKKAHQKGL
                     AVQAWVVVFNATPREQSYIQQNYIYNNHKDWITYNFNDSQMNIDRQSGYFIDPGIPEV
                     QEYLLNILGNLAGGYPELDGIHLDYIRYPESDLGFHPVSLARYNEYCQNQEEITYNEW
                     RIMQVTNFVENAYFQLKEINPALQLTAAVFPDIAEANVDYAQDWQSWLKKGIIDRVYP
                     MAYDVQYAKFKKQLEQINLLQMKEKIVIGLRAWNGNGNSLAVGNGNSYNVKDIAKKIT
                     LTRDYGFAGVSLFSYSGLQKGNAFVQLARLSFPEKKEYPKENISPKLNEITFSEPKYP
                     AEAKLTRLNSEIIMHLKIPKDGLWNWELYKDTLLVSNQRFYQKGENREILNIINPQIS
                     ANLEPGHYIVHLYQEKSSFQYFIPVELVTPNE"
     gene            complement(9304..10644)
                     /gene="xseA"
                     /locus_tag="PLG69_05085"
     CDS             complement(9304..10644)
                     /gene="xseA"
                     /locus_tag="PLG69_05085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPI42918.1"
                     /translation="MENVIIYSVYEITRQLRQVIETSLQPVFVMGEISGFIRHSSGHI
                     YFNLKDENATLRCTFFKGANASLTFQPADGMQVICFGKLTVYEKGGYYNLNVQNMQEA
                     GMGLLQQKFEQLKKKLEKEGLFAAEHKKPLPKYPDRIGIITSPTGAALQDIINIIKRR
                     YPVELIVYPALVQGSDAPPQIIQGIKYFNKTRDVDFIIITRGGGSQEDLFCFNDEALA
                     RAIYDSSLPVISAVGHEIDFTLADFVADLRAPTPSAAAELAVPDKKDLLNLLASLKKR
                     LDLSVQNNFTGKRTPLLNLQLELAKYHPERVWRGFQQRFDMAEMALHNALKQLPEKRL
                     QFEMKANNIMQQLNSRIELCLAKSKADCNLTWHQLNEKINNQLQFKTNRLNSLSEILE
                     QLSPRSIQSKGWIMAFKEGKLISSIKQVSEGENLSLDFADGIAETTVNKVEKRE"
BASE COUNT         3447 a         2062 c         2056 g         3393 t
ORIGIN      
        1 ctttatcatt ataccagtaa tggaattgat cctgccggta gctgggattt aatgtgttcc
       61 gaccaaaatc ctcctcaaca tatgaccgct tttatgaaat ggaaatatgg aaattggatt
      121 gctactattc caactatctt cattggacaa caatataccc taaatccttt aacttcttcc
      181 accggtaact gttatagaat agattccaat aaccccaatc aatattatgt agtagaattc
      241 cgcaagaaaa caggcacctt tgaaagcagt attcccggtt ccggaatgtt gatttataga
      301 attgatacta ccgctggaga cggaaatgca tcaggtccgc ctgatgaatt atatatttac
      361 cgtcccaatg gaacaactac agtaaacgga actataaatt ccgcaaattt tagtcaggaa
      421 accggtagaa ccagtattaa taatacaact aatcctgctc ccttcttaca ggatgggagt
      481 gaagggaatt taaatatcta tgccatcggt tcttcagcag gaactacgat gactttttct
      541 ttaggtgttc ctacggttga cttttcaacc aatccttaca cggagagctt tgacagttct
      601 acttttcctc caacgggctg gtataaacag acaatttcag gcagctatgg ttttgaaaga
      661 gtaacttccg gaacacatcc aacctgctca cctcaagcag gagcaggaat gatttgctat
      721 aaaagttata atgctctttc gtccaattcc gcaattttag ctactttgcg gcttaattgt
      781 tcagaagttc aattttacag ctacgatttt tccttttata tgtataggga taccggaaaa
      841 agttcaaatg ctgaccgtat tttaatttat ttagctgata cgcctgatat gagcggaacc
      901 aatactcttt taggaacgat aaatcgttat accggtttta ccccaaccgt tccttcaaac
      961 ggttggtatc agtattcttt caatttgcct cttacaactt ccggttatta ttatgttgtt
     1021 tgtcaagcta tcagtgccaa aggcaataat atgtttttgg atagtttcag tattaaaaaa
     1081 acggctttac ctccttctcc cgcaatttct ccttcaccag ctgatttagc tgaaaatgta
     1141 agtgtttcac ccattttttc ctgggctaat ggaggcggtt atcccagcca ttatttattt
     1201 tatttgggca cgaataatcc accgactaat ttgattgatg gtttgaattt gggcaatgtt
     1261 ctcaattacg catataccgg aactttgaat tacagcacac aatattattg gaaagttatt
     1321 cccgtaagtg aagggggctc tgcacaaaac tgttctgtat ggagttttac tacttatgct
     1381 gatccccgaa tcacaactct tccctataga gagaattttg atatagtaac tgctccaact
     1441 ttgccttttg gttgggagac ctatatttct tcaagcaata gttctgcttt tgttaaaaac
     1501 ttttcttcgc caacaaatta tgctcttagc cctcctaatt gcatcaattt taataatttc
     1561 acagatacaa gtgcacagct gattctgatt acccccgaga ttttaacgcc gataaatatt
     1621 ttgcgtttga agttttatgc caagggtagc gcaaccggat attcattaca agttggaact
     1681 atgagcagtc ctaacggcac ttttacttca ttaactacgg ttaatttaac caatacgatg
     1741 caacaatatt ctgtgccttt ttccaattac aacggaacag acagatttat agccatcaga
     1801 cataatttag gcggttccaa tcgtaatatt tatctggata atttttatct ggaagaagag
     1861 ctgactaatg acctggctat aagtgctttg cagatgaacg gattaggagt tattggcgaa
     1921 cccttgcagt ttagcgttag tgttcttaat aatggaattg caactcaaaa cagctatagt
     1981 attcagttac tttccaactc cgctaaaaca gtgctggcaa atttggatgt attggaaccg
     2041 cttgctccag ggcaaactgc agttcataat ttaacttgga tacctgaaac cgaacaaacg
     2101 ctttctgtat atgctcaagt aactttaatt tcagatggtt ataccggtaa taatacttcc
     2161 ataccttctc ctatatccat tggaagttac atccctaaaa tagggacaac cgataccggc
     2221 actaaaacta atcttctacc tttaaatttc aatcagaaaa acagtatcag tgagacaatt
     2281 tatcttgctt ccgaactgca aatgactgcc ggaataatta ccgccattgc ttaccataca
     2341 aattttgtgc aggatttatc tcatcaggca gtaaaaatct ggatgaaaaa tacccctgag
     2401 agtaatctca gttccggttg gctttccttt gatgagtatg tgccggtatt tgatggtttt
     2461 gtagattttc ctgctggaag caataccatt attataatct tacaaatacc ttttccctac
     2521 aatggaaata atttagctat cagggtaaac agaccctttg ataacaataa ttacaatacc
     2581 agtaatcatt tttattacac aaattctacc tcaaatcctg gtcgcagccg ctatctaagt
     2641 aacagttctg taatttatga tcctcaaaat ccatcggctt tgggcagttt aagcaatttt
     2701 attcctgtta ccggcttttc tgtaacttcc tatattccag ccgtattagc tattcctcag
     2761 ctgctaattc agaaaaacca acagggtgta ttacttaact ggaatgccgt gccgggagca
     2821 aatttatacc gtatttacgc cagttccgat cctgtggtct ggtctgatat tccttatgcc
     2881 gaatgcagtc aaaccagttt tctgattact aatccaccgg acaaaatgtt tttcaaaatt
     2941 gtagctgttt accaagcacc ctgagttatt aaatccttat gtgcattgtt tggaataaaa
     3001 ttctcatttc gtatgataaa tatgtttatt aattacatac tattttgggc ttgacagaaa
     3061 aagggtttat tttaggaaag ttcaaaagtg tggcgagata gctcagtcgg tagagcagag
     3121 gcctgaaaag ccttgtgtcc ccagttcgat tctgggtttc gccaccattt ttttgtatag
     3181 ttcaattttt ctgaattata gagttgaaga aaatgaaagt tatttttgca gaatgttttg
     3241 gtaaatagcg gttatctttt tacatattac aggaggggaa tagtttgtaa tacatatttc
     3301 tcggatccgg gcaggggaat agttattaat gttttctttc attttcagca ttgcttccgc
     3361 tatcagaaag ggcttttttg gaggaataag aattcctgtt tccggggtaa cgatatctct
     3421 tggaccacca caatcagttg ccaaagttgg ttttccacaa gccattgctt ccagaagaac
     3481 caaactgaag gattcaatat ggctgggaag aatgtagcaa tcgcattttt gatattgttc
     3541 caatgcttgc tgagggttaa gatagcccaa aagatgtaag cggtgttcca aatggtattt
     3601 agttattttt tgtctaattt tagaccttaa atagccatct cctccgaggt ataaatcggc
     3661 atctaatcct ttttgaaccg ccaaagcaaa ggcatctacc aaatccataa ctcccttagc
     3721 ataagttaaa atgcttaaag aaaagaaaga aaagcatttt ccctcttctt tatctgccgg
     3781 ttggtaaaat tccgtatcca ctaaattggg gattactata ctttgaacgg agcaggaaga
     3841 aagaatttgt gcttggagaa atctgcttac agccaaaaca gcggacgcat tgtgtaacgg
     3901 caaagtgatt tttttagaga ccttattcct gataacatat tccggaaaag gaaatggacc
     3961 gctatgttca ctaatgatat agggaatttt ccatttttcc gctaagcgaa aagcaagaaa
     4021 tcctgccgga aaagttacat aggaatgaat aatatcaggt cgggaaagag aatccaattt
     4081 tttcactaat gcttttatat tgccataaaa taacctgctg gtccaggtaa gatgagggat
     4141 tttaatttct ttcaggttgg gggttatatt atgggttctg gttttggcgg ttaggtaatt
     4201 aacaatcttg gagacagcaa caaagggatg tcggatttgc aattgatatt cattctgtcc
     4261 ccaattcagg atttcatatt catagttgct gtttcgggaa agagcaattg cctggtcaag
     4321 aaagaatctt ccagtattgg gtttttgcgg atagggatac caggaaggga taattaagac
     4381 cttcattgtt ccaatgcaga aaagacctca gccagttttg ccgtaagtgc cttgcgttca
     4441 aattgctgaa tgtaatccca gttgggttca gtttgcagct tgtcttcctc ccaaagccga
     4501 taaaggtttc ctaaggtttc ctttatagaa tggatggaag aggaatcggc aataaaagct
     4561 gtatttgcgg gcttaaggat atctgctgct gcccctgagg aatctattat agccagaatc
     4621 ggttttccgc tgcgcagata ttcaaaaacc ttagctgtaa cgattgctct ttgagcttct
     4681 ccggaaccca aatacaataa aagaacatct gcagcacgaa gaatttgaat gctattccga
     4741 tgattgatat aaccgtggaa atggacaatt tgatttagta ttggatcggt tatatatttt
     4801 cccagcacga agttacgaaa gtttctgcca taaatttcca cggcaatttt ttgggggtta
     4861 agacaacctt cctgaattaa ctctaaaatt gcctgccaga gaatatcagg ttggagttca
     4921 tcgtaaaaac taccggtgta aataatggta aatttggcat tccttattgg aggaaatgaa
     4981 acggggaaat ctttctcatc atagccattg gtgatacata cgaatttgtt tttatctaaa
     5041 tagggataaa gatgtctgaa ttcctctatc ataacggaac ttacacagat aacttgatca
     5101 gcacgggtta ctatttcctt ttcccacctg ttctctattg cggtaaccca atcaggaggt
     5161 tttagatatt ttctgccaat accaaaactc caggggtctc taaaatccag gcagaccttt
     5221 atctgataat gctttttcag gtatttggct aaaaagataa gagaataggg cggaactgaa
     5281 actaaaacct gattgatatt gttggtagcc ataattttct ttagcttatg aatcgcaaac
     5341 ggtaaccaca ggatttctcc gtccggaata aaaacatttc tgcgcaggaa attgactatt
     5401 ttgtttagct ttaagcccca aagcagttta taaaaccagt taatatccaa agtgaaagag
     5461 cggtaaattt ttactttatc aggaagttca cgcagaagag tataatccct tggttgcttt
     5521 aaaaagcgat aataagcaca aagaatgaca ggtttaaaac caaattccgg taaatattta
     5581 gcaaacttca gtcctctttg cactccggaa ccgcctgcag gagggaaata atagaaaatt
     5641 aagagcacat ttttcatagt tcttgtgttt tcctgataaa taaacccata taccaataag
     5701 gtcggggatg cactaataac ctgttaagta agttaattcc tttggtgaga agggatttgt
     5761 ttatttcaaa cttttcttgc tgacaacggg aacatttcaa accgctggca ggattaaacc
     5821 attgttttaa ccagtgttgt ttgatattaa gcaaaagggg tataaaatca cgattcagag
     5881 gaccaaatac ctcccttttt ttcagataaa attccggagc aagatatgta gataaagttt
     5941 cttctgaaaa cgactgtaaa tgtccataag gatgttctat aaaaccgcaa ttagcacagc
     6001 gataatatag tttttccagc tgttccttat ggggaacact gataattata tattttttgg
     6061 ttacccgttt aagttccgga aaagctttct gcaaggagac attatccaag tgttccaaga
     6121 cctcgttaca gattacagca tcaaaacttc tatcgggaaa aggaatttct gtaacagaag
     6181 cttgaactga aggacactgc aaatacttta gagctgcggg agaaatatcc aaacctgtaa
     6241 cttgatattt agcaataagt ttgtttgtaa taatgccatt tccacaacct gcgtccaaaa
     6301 tggtttgcac atctgaagga agaagagcaa gaagttttgc aattcttaaa tccgcttgtt
     6361 tttcaggatc gtaattttcc catgtggctt ctgccatata gttcattttt taaacctact
     6421 aaagaaatcg gttaacattt ctcttgctaa ttgcacatca taatccaaaa ttaccccgat
     6481 ttttctacct aaaaagaaat aggcaatcgt aaaaacaaca cctcctgtaa gtaaaaaggg
     6541 aattgtgcta tttcctagct ttattatcag cacgcagatt atagcgcata taactgttaa
     6601 aatggcattt ttagctattt ttaccagtgg gaaaaaagcc attaagttca attttaagac
     6661 cttggcaatc tgaataagat aaataattac cgagagccag gttacaatta ccgtagcaaa
     6721 agcggcacct ttcatcccga atttaattat cagaagatag tttaagatag cattggaaat
     6781 gagcagaaac aaagaattat acataacaaa gcgggttttt ccgaatgcct gaaacagaat
     6841 tccgtaagtg gcaatgcgca aaggcaacaa aaagaggtaa attctaaaat aaatagcgga
     6901 ctcggtatag agctttccat aaataagagt aattgcaggt tctgcaaaaa tataacacaa
     6961 agccattaac ggaaatacta aaatagtatt tttacgaacc gctccgctat ataaaatacc
     7021 catcttttca ggattactgg aactcaaatg aggcaacaaa acagaattta tagagttagc
     7081 taaaattcct acaatcggga attcagtagc tcctatagaa aacacggcaa acttttccgg
     7141 cgagaaaaat gccgaaacca taaatttatc cagttgaata ctcatcatac caattataga
     7201 agataaaccc aagggtaaag aataactcat aagtgttttg aaagagccga gatcaaaacg
     7261 agtttctttg gctttaaatt tggcaagaga aatcctggca taaagccatt gcaaaaaagc
     7321 agaactaatt acaccgcaaa caataatagt tagattacgc gtaagaaaag cggaaccaac
     7381 aatcagaata aaatccgcaa taatggcaaa aatggtaaag gtagccgctt taaacggttg
     7441 tttcaagccc agcattacgg aactgaaaaa ttgggttaca aaaagaaaaa tgggataaag
     7501 agcataaaga ataagtagtt tttccagagc aggattattg aataaagtag caattggttt
     7561 acggaaaata agcaggcaaa gcgcaaaaat taaacccaac agcgaaatta aatttaggca
     7621 acgggtaatt aaggcatttt gtttttccgg actttccgct ttaggaagaa aatagagcat
     7681 actttgagga atccctaaaa gcagaagagt ggaaaaggta gtataaatta agaaaagctg
     7741 acgataactg ccaagttcgg aaggagttaa taaatgagct aaggcaattc cgattacagc
     7801 tttaattgcc aaacggaaaa attccgttgc cgaaaggata cctgcctgtt taggaagctc
     7861 actcattggg cgtaactaat tctaccggaa taaagtattg aaaagaggat ttttcctgat
     7921 acagatggac tatatagtgt cccggttcaa ggttagcgct tatttgggga ttgatgatat
     7981 tcaatatctc tctattttct cctttctgat aaaagcgttg gttgctaaca agcagtgtat
     8041 ctttatatag ttcccagttc cataatccat ctttggggat ttttaggtgc ataatgattt
     8101 cactgttcaa acgggtcagt ttggcttcag caggatattt tggttcggag aaagttattt
     8161 cgtttagttt aggggagata ttttccttgg gatattcctt tttttctgga aaacttaagc
     8221 gagcaagctg aacaaaagca tttccttttt gcaatcctga atagctgaat aaagaaacac
     8281 cagcaaaacc ataatcacgc gttaaagtaa ttttctttgc tatatctttt acattgtagg
     8341 aattaccatt tccgacagca agggaatttc catttccatt ccaggcacgc aaaccgatta
     8401 ctattttttc cttcatttgc agaaggttaa tttgttccag ttgtttcttg aattttgcat
     8461 attggacatc ataagccata ggataaacac gatcaataat acctttttta agccaggatt
     8521 gccagtcctg agcataatca acatttgcct cggcaatatc gggaaaaaca gcagcagtta
     8581 attgcaaagc aggatttatt tctttcagtt gaaagtaagc attttccaca aaattggtta
     8641 cttgcattat gcgccattcg ttataggtta tttcttcctg attttggcaa tattcattat
     8701 agcgggcaag agagacagga tgaaaaccta aatcgctttc aggatagcga atataatcaa
     8761 gatgaattcc atccaattcc ggataacctc ctgccagatt tcctaaaata ttaagcagat
     8821 attcctgcac ttcaggaatt cccggatcaa tgaaataacc ggattggcgg tcaatattca
     8881 tctgagaatc gttgaaattg taagtaatcc aatccttgtg attattgtag atatagtttt
     8941 gctgaatata actttgttcc cttggagtag cattaaaaac cactacccag gcctgcactg
     9001 ccagtccttt ttgatgagct tttttcagaa tataagctaa aggatcaaaa gaagcatctt
     9061 ccaggatata actcttcggt tcgggattgg gataaagata ggcacctttg gatgtatcaa
     9121 aaagagcatc agcgcgataa cgaacttcca ctaaaagttc attttggttg caagaaaccg
     9181 ctgttgcaat tacttcatca atagattttt cggtagtaat atcccaaggt agaacccaaa
     9241 cggaacggat ttcggcattt attttcacaa acaaaatggt aaacagaata aaaaaaccct
     9301 ttttcactct cttttttcta ccttatttac agtcgtttcc gcaatgccat cggcaaaatc
     9361 cagggagaga ttttcccctt cagaaacctg ttttatggag ctaattaatt taccttcttt
     9421 gaaagccata atccaacctt tactttgaat actgcgagga gagagttgtt ctaaaatttc
     9481 gcttagggaa ttcaggcgat tggttttgaa ttggagttgg ttattaattt tttcattcag
     9541 ctgatgccag gttaaattac aatcggcttt ggatttagcc aaacagagtt caattctgct
     9601 attcagttgt tgcataatgt tatttgcttt catttcaaat tgtaaacgct tttccggaag
     9661 ttgtttaagt gcattgtgta aagccatttc tgccatatca aagcgctgtt gaaaacctct
     9721 ccaaacgcgt tccggatggt attttgccag ctccaattgc aaatttaaga ggggagtgcg
     9781 ttttccggta aaattatttt ggacagataa atccagccgt tttttcagcg aagcaagcaa
     9841 gtttaacagg tcttttttat cgggaactgc aagttcagcc gcagctgaag gagtgggagc
     9901 tctcaaatcg gctacaaaat cagccagcgt aaaatcaatt tcatgaccta ctgccgaaat
     9961 tacaggaagg gaagaatcgt aaatggctct ggctaatgct tcatcattaa aacagaaaag
    10021 atcttcctga gaaccgccac cacgagtgat aattataaaa tcaacatccc tggtcttatt
    10081 gaaatacttt attccctgaa taatttgagg aggagcatca gaaccttgaa ccagagcggg
    10141 ataaactatt aattccacag gatagcgtcg ttttatgata tttattatat cctgaagcgc
    10201 tgcacctgta ggagaagtga ttattccaat gcggtcagga tatttgggaa ggggcttttt
    10261 atgctctgct gcaaaaagtc cttccttttc cagtttcttt ttcagctgtt caaatttctg
    10321 ctgtaaaagt cccattcctg cttcttgcat attttgcaca ttcagattat aataaccgcc
    10381 tttttcgtaa acagttagct tgccgaaaca gataacctgc attccatcag cgggttgaaa
    10441 agttaaagaa gcattggcac ccttgaaaaa agtgcagcgt aaggtggcat tttcatcctt
    10501 caggttaaaa tagatatgac cagaactatg acgaataaaa ccagaaattt cacccattac
    10561 aaataccggt tgcagggatg tttcaatcac ctgacgcagc tggcgggtta tttcataaac
    10621 gctatagatt attacatttt ccatttcaat gccaaaatct aaacataacg ctatttgtca
    10681 attctaatac catctttttc ttgatgagtc ctttatgtca gaaaaaatta tttccttttg
    10741 gaaaatacta ttattgttaa aacctctatg gatttctgat tttacaggaa tagcaaaaaa
    10801 tatatttctg cttttgcaag aatgacattt gaacgacaga tactataaaa gggtttcaac
    10861 ccggtgaaaa gtaagcagga gaatcattat ctctttttcc gagaagaagt tccgcaggaa
    10921 cgacaggtat taacaacggg tttcaacccg gtgaaaag
//
LOCUS       DAOPFL010000055        10727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_7518_length_10727_cov_83.335645, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000055 DAOPFL010000000
VERSION     DAOPFL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7518_length_10727_cov_83.335645"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1033)
                     /gene="murI"
                     /locus_tag="PLG69_05090"
     CDS             complement(230..1033)
                     /gene="murI"
                     /locus_tag="PLG69_05090"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPI42919.1"
                     /translation="MKRAIGIFDSGVGGLTVYKAIRNTFPNEDIIYFGDTARVPYGPK
                     SPNTIIDYSLQNARFLLQQGIKILIVACNTSSAVAIPALQNLSDIPVIGVIEPGAEQS
                     VKVTKCKRIGVIGTEGTIRSGAYPSAIRKLLPEAFVVSSACPLFVPLVEEGWQEHPVA
                     KVIAEEYLKEMKEQNIDTLVLGCTHYPLLSKTIQQVMGEEVTLVDSAMAVAEYLSRYL
                     SDENDGQKGRDSFYVSDNENKFAEIAERILQHNIGNLTRVRLYESWFKD"
     gene            complement(1030..3321)
                     /locus_tag="PLG69_05095"
     CDS             complement(1030..3321)
                     /locus_tag="PLG69_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HPI42920.1"
                     /translation="MNYNELKQAILEYYQKYPHTGLSYNEIVNTFQLNKAEKALLSGI
                     LSGLMEEGILSKDRKKYHLQKMPKAKQPSPTPNPKLLQGIFDATPLSRGFSYAFIRTP
                     EKDYYVDAEDILNAFHNDIVAFEPKYRKGHQDSAIIRKVVKRSTEQFVGELIRRNGFS
                     TFICSNPKVFRWFFVSDPAQGENGDKVVLKVTNWGNPQMGKVPVGKVIEILGKSGEPE
                     VELLAVIRQYNLPLEFPEDVIAEANLLSAEIKPEDYRNRRDLRNLYSFTIDPITAKDF
                     DDAIAVEKTAKGWRLYVHIADVAHYLSVEGAIFQEASKRGNSFYFPKRVIPMLPERLS
                     NLICSLRPEEEKLCLTVQTDFDRKGQIIEQQIYESVICSQSRLNYDEVDDLFTGKKTE
                     IPIPLQDILLEARALSRLLTEKRMNAGYIFFDLPEIEYEYDENGFLKCLTLAEETESH
                     KIIENFMLVANEYVATRLSQTAPATIYRIHNDPDFNKLERLIELLSYYGVKWVMYENL
                     NKSIQYLLNSFPNEAYHKVFDRILLRSMKKARYSTEHIHHFALAMENYTHFTSPIRRL
                     CDLVVHYLCKTWLCHTANQKFTPSQLKHFAEVASEQEIQADQSERDIEMVYSLALMRE
                     KEGELYEGMVISVNSRGLIVQLEEIPVTGVIKEISLGEGNWEYLEREMRYINRRTHKY
                     YQLMDTLTVIIGYVEDDIYLFPIPETLRHNVSKTKSSLSHKSALQKPKPSGRTRSTSS
                     QRTGRTRSPSSKSSKYSNTRGNK"
     gene            complement(3330..3767)
                     /locus_tag="PLG69_05100"
     CDS             complement(3330..3767)
                     /locus_tag="PLG69_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42921.1"
                     /translation="MLPEPILKWKSWPLVERPWTSLILILFLILLSFLLYYITVTGWQ
                     QPFYYILGMLLVLGNLLPYFIMTEYELYEDKVQVRYLFLKISRPYTDFGCFYEDKRGI
                     MLSTFTMPRRLDPFRGLSLRFSADKQEREKLIEILMQKIGKKY"
     gene            complement(3757..4632)
                     /locus_tag="PLG69_05105"
     CDS             complement(3757..4632)
                     /locus_tag="PLG69_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPI42922.1"
                     /translation="MKSMTGFGNALISRDNIDLELEIKSINARFLDLRIFLPRELSFY
                     ETEIRRQIIKSISRGAIEVRVNFSDHREPKLVLDKTKLLKYNELAIEAAQLLASEEVI
                     SIEFLLQEPGVIESVDRLAEDEILANVLNEALAQALQRIKESMLEEGEQIKQVLCDSM
                     QQIEKAINAVSELCEPFKKELFATMHKHIEELIGSYKVENLEQRLIQELAIYVDKYDI
                     GEELSRLRAHLKTFTSTLQEEGDIGKTLNFIIQEMQREANTLGSKFSTTQSFPWILTI
                     KEEIEKCREIIQNVA"
     gene            complement(4874..6157)
                     /gene="miaB"
                     /locus_tag="PLG69_05110"
     CDS             complement(4874..6157)
                     /gene="miaB"
                     /locus_tag="PLG69_05110"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPI42923.1"
                     /translation="MKFYIETYGCQMNVADSELIASILTKAGHQEVHQISKADLLLFN
                     TCSVRGHAEERVLGRIQSENHRKKENPNLKIGVVGCMAQRLGEEINKEKLIVDFVIGV
                     DQYQHLPDILNEETEKTILTDLDETQLYKGIQPVYHNDYCAYITIMRGCNNFCSYCIV
                     PYVRGRERSRSWQEIIEETISAGKQGKKDITLLGQNVNSYLNGEVNFPSLLVKLNELD
                     EIYRLRFITSHPKDLSEELIEVLANSAKICEHIHLPVQSGSDNILKAMNRNYTVQHYI
                     SLVEKLHKFIPNIAITTDIMTGFPGETENDFQDTLSLMKTIEFDDAFCYKFSPRPGTV
                     AETLSNQVPEAERLARLQQMIELQRKISLKKNREQIGRKVEVYIESFSKKSRKMVLGK
                     TRDFKTAVLPGTEDDFGTLKQIEVKDATASTLICF"
     gene            complement(6154..8229)
                     /locus_tag="PLG69_05115"
     CDS             complement(6154..8229)
                     /locus_tag="PLG69_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42924.1"
                     /translation="MLKFALYVSNHGFGHATRICALAEELIHYGVFCHIITTKPAFLF
                     QDLNPHYFVLHNRAIDVGVKHSENLTVDIKRTINSLLELLSKRNEIMEREIEFLRREK
                     IDCIIADIPFLVSDFAAYCHIPVFAVTNFEWHFIYSSLLAKEENTEKENGIVNNYNNL
                     ELSGGAASSGYTNFSTPEVANSLKPVLNLIWSLYQRFDGCFRLPFSTDESMSAFKDYI
                     SCGLLSRKKDKYKDIRLQYNLPKETPILLVMFGGEGTMELNYEALCSAYQGIVISTQA
                     GIKAKNHIQVSMDDDFPDFIYNADIILCKPGYSTLAEVVQAGKFIIYCPRSNYPEEKA
                     LIAGLKNYTNCLQIDTLQLSAGQWKAIFEDIKPRKIRTKTYKNCNTEVAGLVLKKYTQ
                     LQNRRLLSVFDLGSNNLNYVLFDLEKRQIVHQTQLTTALGIAFKHNKISEKRLTSVKQ
                     IISKLLQLDSYLESEKVLLATGVMRFALNANLLTDWITDKYNIKCRIISSQEEIRYAY
                     FSARQYRSGEEATLAIDIGGFSTEFINLDKEKKQQGNSLPFGLLTLLNDFNGDSETAD
                     NFIRQKLNDLPYKGSYCLVGIGLTYTYLAGVIFRCGYNEREKMEGKTITKKQLQELSH
                     SLEVRDEQRYLPFLLEESYLPILKLSIAFNISLLDRFETSEIIVCNSGIAVGYAYWYS
                     SKQRSKAQK"
     gene            complement(8239..8607)
                     /locus_tag="PLG69_05120"
     CDS             complement(8239..8607)
                     /locus_tag="PLG69_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPI42925.1"
                     /translation="MKKYSILNDLKPADVVLILLIILAIFLSARYYLSNKPHSQVYIY
                     KDNQLFGVYSLEQNKVIKIDEHNTVEIKNGKVRMKFADCPDKRCVKQGFTKSMPIICL
                     PNKLVIEIQDNGKQKKLILQ"
     gene            complement(8608..9471)
                     /gene="amrS"
                     /locus_tag="PLG69_05125"
     CDS             complement(8608..9471)
                     /gene="amrS"
                     /locus_tag="PLG69_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPI42926.1"
                     /translation="MREAMYYVREGNKIRCQLCPRECVLSEGKKGFCRSREVINGSLI
                     ATAYGRTTSLALDPIEKKPLYHFHPGTMIVSLGPNSCNLTCKFCQNWEISQQEYPTRY
                     VAIEDLIEIVKQQKDQQVAFTYTEPLMWYEYILDFSAKAPEIDIVLVTNAYINKEPWR
                     NILKVVKAVNIDIKSYRDEFYRQLCGGKLDIILDNIIIAKEMGVHIELTNLIIPGYNN
                     SEEELNDLAKFIASVDKNIPLHISAYRPCYKMTVRPTTREEVEHACEIASQYLTYVYA
                     GNVYSSRFSRR"
     gene            complement(9471..10613)
                     /locus_tag="PLG69_05130"
     CDS             complement(9471..10613)
                     /locus_tag="PLG69_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPI42927.1"
                     /translation="MASIKAEGEDIHKGILFHSASMGELTAIKALVTRHLQEHPEVKL
                     CITTSTVTSCAEANKISPKVKGFLSVLDLPHLRNRQLKRINPGLICIVETEIWPNMLL
                     WAKRNKVPVLFLNARMSKSTLRGYRLLKFLFRHLQSPITEIHAQSKDDAKRYRKIFNR
                     PVYVSGNLKFSLTLKDYNAEELRKKWGYKSDDFIICWGSSRPGEEALLISLYPSLKEV
                     IPNLHLIIGLRHPRRLEEVIELMDNYSYSCFTMRKEYSRAEDILIIDTLGILDQAYCI
                     CDIAIVGGSFYDFGGHNPLEPAYYKKPVVIGPHHYSCKESVKKLKVAGGIIISNKDDL
                     LADLIALYKNKELREKMGKAAKKVLTENAHTLDIYLKALEKWLKEE"
BASE COUNT         3228 a         2106 c         1797 g         3596 t
ORIGIN      
        1 gcagcagtta aaattaaggg ttccataatg ttacctcttt atatttaatc tatttatcta
       61 aaatataata tctaatgctt tgaaattatg ctcgttccca atagcattac tttagcaata
      121 ctcacccatt gctaatggca ttagtaatgg gtaagcaatg agtaagcaat gctattaagg
      181 aagaaacttt tcttattcat ttttatattt ttacgcatca taattttgct cagtccttaa
      241 accagctttc ataaagacgc acccgggtta agttaccaat attatgctgc aaaatgcgtt
      301 cggcaatttc ggcaaattta ttttcgttgt cgctcacata aaaagaatct ctgccttttt
      361 gtccatcatt ttcatcactc agataacggc ttaagtattc agccacagcc attgcgctat
      421 ctacaagtgt aacttcttct cccataacct gttgaatggt tttagatagt aagggataat
      481 gggtacaacc taaaacaaga gtatctatat tctgttcttt catttcctta agatattcct
      541 ccgcaataac tttcgctaca ggatgctcct gccagccctc ttccaccaac ggaacaaaaa
      601 ggggacaggc agatgaaact acaaatgcct caggaagcaa ttttctgata gcggaaggat
      661 aagctccgct tcggattgtt ccttccgttc caatcactcc aatgcgttta catttagtaa
      721 ctttaaccga ttgttcagca cctggttcaa taacacctat aactggaata tcgcttaaat
      781 tttgcaaagc gggaattgct actgcggaag atgtgttgca agcaacaatt aagattttta
      841 ttccctgctg gagcaaaaaa cgggcattct gcagggaata gtcaataatg gtattaggag
      901 attttggtcc gtaaggcact ctggcagtat ctccaaaata aataatatcc tcattgggaa
      961 aagtattacg gatggcttta taaacggtta agccaccaac tccagaatca aaaataccga
     1021 ttgctctttt catttattgc ctcttgtgtt tgaatatttg gaggatttgg acgatgggga
     1081 cctcgttctt ccggttcttt gggacgatgt ggacctcgtc cttccagagg gttttggctt
     1141 ttgtaatgcg cttttatggg ataaggaact ctttgttttg gatacattat gacgcagtgt
     1201 ttcaggtatg ggaaacaaat aaatatcatc ttccacataa ccaataataa ccgttaaagt
     1261 atccattaat tgataatatt tatgagttct gcggtttata tagcgcattt ctctttccaa
     1321 atattcccaa ttaccttctc ccaaactgat ttctttaata actccggtaa ccggaatttc
     1381 ttctaattga acaattaaac cccgggaatt gaccgaaata accattccct cataaagttc
     1441 tccttccttc tctctcatca aagccaagct ataaaccatt tctatatcgc gttctgactg
     1501 gtctgcctga atttcttgtt cagaggcaac ttcggcaaaa tgtttcagtt gagaaggagt
     1561 aaatttttga ttagcggtat gacataacca ggttttacag agataatgaa caaccaaatc
     1621 gcaaagacgt cggataggac tggtaaaatg tgtgtaattt tccattgcta aagcgaaatg
     1681 atgaatatgt tccgtgctgt atcttgcttt tttcatactt ctaagcaaaa tgcggtcaaa
     1741 aaccttatga tatgcctcat tcggaaaaga attaagcaaa tactggatgg acttattcaa
     1801 attctcatac ataacccact ttacgccata ataggaaaga agttcaatca gccgttccag
     1861 cttgttaaaa tcggggtcgt tatgaatacg gtaaattgtt gcaggagccg tctgtgaaag
     1921 gcgtgtagct acatattcat tggcaaccag cataaaattt tctattattt tatggctttc
     1981 tgtttcctca gccaaggtta aacacttaag aaatccgttt tcatcatatt catactcaat
     2041 ttccggtaaa tcaaaaaaaa tgtagccagc attcattctt ttttcggtta aaagacgcga
     2101 aagagctctt gcttccaata aaatgtcctg taaaggaatc ggtatctccg ttttcttgcc
     2161 agtaaaaagg tcatccactt catcataatt aagccgggat tgactgcaaa taacgctttc
     2221 atatatttgc tgctcaataa tttgtccttt acggtcaaaa tccgtttgca ctgttaaaca
     2281 gagcttttct tcttcgggac gtaaactgca aataagattt gataagcgtt ccggtaacat
     2341 aggtataact ctcttgggaa aataaaaact attcccgcgt ttgcttgctt cctgaaaaat
     2401 agccccctcc actgacaaat aatgtgctac atcagcgata tgcacataaa gacgccagcc
     2461 cttagccgtt ttttccactg ctatggcatc atcaaaatct ttagcagtaa tcgggtctat
     2521 agtaaaggaa taaagattac gcaaatccct ccgattcctg taatcctccg gttttatttc
     2581 tgcagaaagc agatttgcct ctgcaattac atcttcagga aattctaaag gtaaattata
     2641 ctgacgaata acagctaaaa gctcaacttc tggctcaccg gattttccta atatctctat
     2701 cactttacct acaggcactt tccccatttg cggatttccc caattagtta cttttaaaac
     2761 aactttatcg ccattttctc cctgagcggg atcggaaaca aagaaccatc tgaatacttt
     2821 ggggttactg caaatgaaag tgctaaaacc attccttctt ataagttctc ccacaaattg
     2881 ctctgtgctt ctttttacta ctttacggat tatagcgcta tcctgatgac cttttctata
     2941 ttttggttca aaggcgacaa tatcattatg aaaagcattt agaatatctt ccgcatctac
     3001 ataataatcc ttttccggag ttctaataaa agcataagaa aaacctctgg ataagggtgt
     3061 agcatcaaaa atgccttgta acaatttggg attgggagtg ggggagggct gttttgcttt
     3121 tggcattttt tgtaagtgat atttcttgcg gtctttgctt aaaatacctt cttccatcag
     3181 tccgctaaga atgccactaa gtaatgcttt ttcagcttta ttaagttgaa aagtatttac
     3241 tatttcattg taggacaatc cagtatgtgg atatttctga taatattcta agattgcctg
     3301 ctttaattca ttataattca taatattcct tagtattttt tcccaatttt ttgcatcagg
     3361 atttcaatta atttttctct ttcttgttta tcggcagaaa atcgtaagga taatcctcta
     3421 aaagggtcta aacgcctggg cattgtaaaa gtggaaagca ttatacctct tttgtcttca
     3481 taaaaacaac caaaatcggt atagggacga ctgattttaa gaaaaagata acgcacctga
     3541 actttgtcct cataaagttc atattcggtc attatgaaat aggggagaag gttacctaaa
     3601 accaaaagca tccccagaat gtagtaaaat ggttgttgcc agccggttac agtgatataa
     3661 tataataaaa aggaaagcaa aatcaagaaa aggattaata ttaacgaagt ccagggacgt
     3721 tctacgagag gccaggattt ccatttaagg attggctcag gcaacattct gaataatctc
     3781 ccgacatttt tcaatttctt ctttaattgt taaaatccaa ggaaaacttt gagttgtaga
     3841 gaattttgaa cccaaagtat ttgcttcgcg ttgcatttcc tgaatgataa agtttagtgt
     3901 tttgccaata tctccttctt cctgaagggt agaagtaaag gtcttaagat gagctctcaa
     3961 acggcttaat tcctcaccaa tatcatattt atctacataa attgccaatt cctggatgag
     4021 gcgttgttcc aaattttcaa ctttataaga accaatgagt tcttcaatat gcttgtgcat
     4081 tgttgcaaat aactcttttt taaaaggttc acaaagctcg gaaacagcat ttattgcctt
     4141 ttcaatctgt tgcatagagt cacataatac ctgttttatt tgttctcctt cttccagcat
     4201 tgattcttta attctttgca gagcttgtgc caatgcctca tttagcacat ttgccaagat
     4261 ttcatcctct gccagacggt ctacactttc aataactccc ggctcttgta gtaaaaattc
     4321 tatggaaata acctcctcgc tggctaaaag ttgtgctgct tctatagcta attcattgta
     4381 ttttaataac ttggtcttat ctaaaaccaa ttttggttct cggtgatcac taaaatttac
     4441 tcttacttca attgctccac gcgatatgct ctttattatc tgccttctta tctccgtttc
     4501 gtaaaaactc agctcgcggg gaaggaaaat ccttaaatcc aaaaagcggg catttatgga
     4561 ttttatctct aattcaaggt caatattgtc tctgctgatt aaagcgtttc caaaaccggt
     4621 catacttttc attataactc ctttttatat tctcgtgtct tgccattctt gttttctttg
     4681 tcattcctgc gcaagcagga atccatttta ttattatgct ttaaaatgga ttctgcaccc
     4741 agtgcgaaat gacaaaaaaa aatgtaaaat gacaacaaca aatgtggggg ataagtaatt
     4801 ataacgcata gtgattaaac cccctgtatg caagtttaca taatatgtct tatcacaact
     4861 attaggcatc tgattaaaag caaataagag tgcttgctgt ggcatccttt acctcaattt
     4921 gtttcaaagt tccaaagtca tcttctgttc ccggaagaac tgcggtttta aaatcgcgtg
     4981 ttttccccaa aaccattttt ctactttttt tgctaaagct ctcaatataa acttccactt
     5041 tgcgtccaat ttgttctctg ttctttttaa gcgagatttt tcgttgcagc tcaatcattt
     5101 gctgtaaacg agccagtcgt tcagcttccg gaacctgatt tgataatgtt tctgcaacag
     5161 ttccgggtcg cggactgaat ttatagcaaa aggcatcatc aaactcaatt gttttcatta
     5221 gggatagtgt gtcctgaaaa tcgttttctg tttctcctgg aaaaccggtc ataatatctg
     5281 tagttattgc tatatttggt atgaacttat gtaacttttc cactaaagat atatagtgct
     5341 gaacagtgta atttcggttc attgctttaa gtatattatc actcccgctt tgcacaggta
     5401 agtgtatatg ttcgcatatt ttagctgaat ttgccagaac ttctattaat tcctcggata
     5461 aatccttcgg atgagaagta ataaatctca accggtagat ttcatccaat tcgttaagtt
     5521 taactaacaa actgggaaaa tttacctctc catttaggta agaattgaca ttttgaccta
     5581 aaagagtaat atcctttttc ccctgtttac cagcagaaat tgtttcctct atgatttcct
     5641 gccaggaacg actgcgttct cttcctctaa cataaggaac gatacaatag gaacaaaaat
     5701 tattacagcc acgcataata gttatatagg cacaataatc attatgataa acaggttgaa
     5761 ttcctttata taattgagtt tcgtccaaat ccgttaaaat ggtcttttct gtttcttcat
     5821 ttagtatatc aggaagatgt tgatactgat ccacaccgat tacaaaatcc actattaact
     5881 tttccttgtt tatctcttcg cccaatcgtt gtgccataca gccaactaca ccaattttga
     5941 gattcggatt ttctttcttg cgatgatttt cactttgaat tctgcctaaa accctctctt
     6001 cagcatggcc acgaacactg caagtattaa atagaagtaa atccgctttg cttatttggt
     6061 gaacttcctg atgacctgct ttagttaaaa tactggcaat taattcacta tctgccacat
     6121 tcatctgaca gccgtaggtc tctatataaa acttcatttt tgtgccttgc ttctttgttt
     6181 actggaatac caataggcat aaccaactgc aattccagag ttgcaaacaa taatttcgga
     6241 ggtctcaaat ctgtccagta aagaaatgtt gaaagcgata cttaatttta atataggcag
     6301 atagctctct tccaatagaa agggtaaata tctctgttcg tctctaactt caagggaatg
     6361 agaaagttcc tgcaattgct ttttagtgat tgtttttcct tccatttttt ctctttcgtt
     6421 ataaccgcag cggaaaataa ctccagccag ataagtgtag gtaagtccta tcccaacaag
     6481 acaataggaa cctttataag gtaaatcatt tagcttttgc cggataaaat tatccgctgt
     6541 ttcactatct ccgttaaaat catttaacaa ggtaagtaaa ccaaagggta aactattccc
     6601 ctgttgtttt ttttctttat ccaagttgat aaactctgtg gaaaagcccc caatatcaat
     6661 tgccagggtt gcctcttccc cacttctata ttgtcttgcg gaaaaataag catagcggat
     6721 ttcctcttgg gaagaaatta ttctgcattt tatgttatat ttatcagtaa tccagtcagt
     6781 taatagattg gcatttaaag cgaaacgcat tacacctgtt gccaaaagaa ccttttcgga
     6841 ttccagatat gaatccaatt ggagcaattt acttattatc tgcttaacac ttgttagccg
     6901 tttttcgctt atcttgttat gcttaaaggc aattcccaag gcagtagtca attgagtttg
     6961 atgaacaatt tgtctttttt caaggtcaaa gagcacataa ttcaggttgt tggaaccaag
     7021 gtcaaacacg ctaagtaatc ttctattttg taattgagta tattttttca gtaccaaacc
     7081 ggcaacttca gtattgcagt ttttatatgt tttagttcta atttttcggg gtttaatgtc
     7141 ctcaaaaata gctttccact gtcctgcact tagttgtaat gtatctatct gcagacaatt
     7201 agtatagttt ttcagtcctg caatcagggc tttttcttca ggataattag agcgtggaca
     7261 atagataata aattttcctg cttggactac ttcagctaaa gtactatatc ctggcttgca
     7321 aagaataata tcggcattgt atataaaatc cggaaaatca tcatccatag atacttgtat
     7381 atggtttttg gcttttatcc ctgcttgtgt ggaaataact attccttgat atgccgaaca
     7441 gagtgcttca taattcagct ccatagttcc ttctccacca aacattacca gtaaaattgg
     7501 tgtttctttg ggtaggttat attgcaatcg gatatcttta tacttatctt ttttgcggga
     7561 aagcaatccg caggaaatat aatctttaaa ggcagacata ctttcatcag tgctgaaagg
     7621 taaacgaaaa caaccatcaa agcgttggta taaagaccag attaaattca ataccggttt
     7681 taaagaattt gcaacttcag gagtggaaaa gtttgtgtag ccggaggacg ctgctcctcc
     7741 acttaattct aagttattat agttattaac tatcccattt tccttttctg tgttttcctc
     7801 ttttgccaaa agagaactat agataaaatg ccattcaaaa ttggtaaccg caaaaacagg
     7861 aatatgacaa taggcggcaa aatcacttac cagaaaaggg atatcggcaa ttatgcaatc
     7921 aatcttctcc cgtcgtaaaa attcaatctc ccgttccata atttcgttgc gtttagagag
     7981 taattccaat aatgaattaa ttgttctttt tatatctacc gtaagatttt cactatgctt
     8041 aactcctaca tctattgcgc ggttgtgtaa aacaaaataa tgcggattga ggtcttgaaa
     8101 aagaaaagcc ggtttagtag taataatatg gcaaaaaaca ccataatgaa taagctcttc
     8161 cgctaatgca catatccggg tagcatgacc aaaaccgtga ttggaaacat acaaggcaaa
     8221 tttcaacatt tatctctcct attgcaggat tagtttcttt tgtttgccgt tatcttgtat
     8281 ttctatcact aatttattag gtaaacaaat tataggcatt gacttggtaa aaccttgttt
     8341 tacacaacgc ttatcaggac aatcggcaaa tttcattcta actttaccat tttttatttc
     8401 cacggtattg tgctcatcaa ttttgatgac tttattctgt tctaaagagt aaaccccaaa
     8461 aagctgattg tccttataaa tataaacttg agaatggggt ttattgctta aatagtatct
     8521 ggcggaaaga aatatggcta atataatcag taatattaaa actacatctg ccggtttcag
     8581 atcgtttaaa atagaatact tcttcatcta tcttcgggaa aaccgtgaag aatatacatt
     8641 acccgcataa acataagtta gatattggga cgcaatttca caagcatgtt ccacttcctc
     8701 tctggtagta ggtctaacgg tcattttgta acaaggacgg taagcagaaa tatgaagcgg
     8761 gatattttta tctaccgaag caataaactt agctaagtca ttcagttctt cttcactgtt
     8821 attgtagccc ggaataatca ggttagtaag ttctatatgg acacccattt ctttggcaat
     8881 tatgatgtta tctaaaataa tatccagttt tcccccacag agctgacggt aaaactcatc
     8941 tctataggat tttatatcta tattaactgc ttttactact tttaaaatat tacgccaggg
     9001 ctctttattt atataggcat tagtaactaa aacaatgtca atttcaggtg ctttagcaga
     9061 aaaatccaga atatattcat accacattaa aggttcagtg taagtgaaag caacctgctg
     9121 atctttttgt tgtttcacaa tttcaatcaa gtcctcaata gccacatagc gggtaggata
     9181 ttcctgctga ctgatttccc aattttgaca aaacttacag gtcaaattgc aggaattggg
     9241 acctaaagaa acaatcatag ttccaggatg gaagtgatag agcggttttt tttctatagg
     9301 atcaagtgcc agagaagttg ttcttccgta agcagtagca attaaagaac cgtttatcac
     9361 ttcccggctt ctgcagaaac cttttttacc ttcgcttaaa acacattccc gggggcaaag
     9421 ttgacagcgg attttattgc cttctcgcac ataatacatt gcttctctca ttattcctcc
     9481 ttcagccatt tttccaatgc ttttaaatat atatccaaag tatgagcatt ttcagtcaag
     9541 accttttttg ccgctttgcc cattttttct cgtaattctt tatttttata aagagcaatc
     9601 agatcggcaa gcagatcgtc tttattgctg ataataattc cccctgctac tttcagtttt
     9661 tttaccgatt ctttacagga atagtgatgc ggaccaatta ccaccggttt tttgtaataa
     9721 gccggttcca gaggattatg ccctccgaaa tcataaaaag aacctcctac aatagcaatg
     9781 tcacagatgc aatatgcctg atcaaggata cctaatgtat ctattatcaa aatatcttct
     9841 gctctgctat attcctttct catagtgaaa caactgtagg aataattatc catcagttct
     9901 ataacttctt ccaggcgtct gggatgcctg agacctatga ttaaatgtaa gttgggaata
     9961 acttctttta gagatggata gagggaaatc aacaatgcct cttcaccggg acgagatgaa
    10021 ccccaacaaa taataaaatc gtcactttta taaccccatt ttttcctgag ctcttcggca
    10081 ttataatcct ttagggttaa cgaaaatttc aggttgccac tcacatatac aggtctatta
    10141 aatattttac gataacgctt ggcatcatct ttactttgcg catggatttc ggtaatagga
    10201 ctctgtaaat gcctaaatag aaatttcaat aaacgataac cccttaaagt gcttttagac
    10261 attctggcat ttaaaaataa aaccggaact ttattgcgtt ttgcccaaag cagcatattg
    10321 ggccaaattt ctgtttccac tatgcaaatc agacctggat tgattcgttt tagttgacga
    10381 ttccgtaaat ggggcaaatc aagaacagat agaaatcctt ttactttagg gcttatttta
    10441 ttggcttcag cacaactggt aacagtgctg gtggtaatac aaagtttaac ttccggatgc
    10501 tcttgtaaat gtcttgtaac caatgcttta atggcagtta attcacccat tgaggcagaa
    10561 tgaaataaaa tccctttgtg aatatcttct ccttcggctt taatgcttgc cacatagttt
    10621 ttctgcttta aaaaaagata gagcaaagga aagaaaagga aatatattgt ttccaccagg
    10681 atattgaaaa gcgatagtaa aatcattttc tattgtgtat ggcaatg
//
LOCUS       DAOPFL010000056        10725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_7523_length_10725_cov_88.386692, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000056 DAOPFL010000000
VERSION     DAOPFL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7523_length_10725_cov_88.386692"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(463..882)
                     /locus_tag="PLG69_05135"
     CDS             complement(463..882)
                     /locus_tag="PLG69_05135"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPI42928.1"
                     /translation="MKEKSLLLIKPNAVRNKHIGHIITILEENNFHFCALKIFRFNRE
                     LAEHFYAEHKGKSFFEKLLNFMMSDFTVAIIVQKDNAVQELRDLIGDVNPEKRKQGTI
                     RYLYAEGITENAVHASDSIDSAKREIGIIFNTENTEK"
     gene            complement(879..1202)
                     /locus_tag="PLG69_05140"
     CDS             complement(879..1202)
                     /locus_tag="PLG69_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42929.1"
                     /translation="MLNHEDLQINYENLLKLGYVVVDIRYKEYDICQETPKLVIARVD
                     SDRDDFYQDMLKLYTGIEFKPNEMYEIWTDILKHKIKMSMVLNRDIAIKVAALDFIET
                     VYTAK"
     gene            1328..3331
                     /gene="uvrB"
                     /locus_tag="PLG69_05145"
     CDS             1328..3331
                     /gene="uvrB"
                     /locus_tag="PLG69_05145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPI42930.1"
                     /translation="MQHFKLISDYTPSGDQPEAIEALIKGIEEGKRHQVLLGVTGSGK
                     TFTIANVIAHFNRPTLVLSHNKTLAAQLYGELKQLFPENAVEYFISYYDYYQPEAYIP
                     GKDIYIEKDSSINEQIEKLRLRATMSLMERRDVIIVSSVSCIYGLGVPEEYREALISL
                     KKGMKMDREELIHQLISAHYSRNDIAFERGAFRVRGDVVDIFPAYLEHCLRVEFFGDE
                     IYRLEKIHPISCQPLEEVENYDVYPAIHFLMNEEKLRKALISIEQEMNERVAWFLQNQ
                     KYVEAERLKQRTTFDLEMLRELGYCSGIENYTRHLTGAKPGEPPNCLLDYFPEDFLMV
                     IDESHVTIPQVHGMFAGDYTRKKNLVEYGFRLPSAFDNRPLRFEEFEKFMHQVIFVSA
                     TPADYELSLTKGEIVEQVIRPTGLLDPEIEIKPIKHQVDDLIAQIKERVAKKQKILVM
                     TLTKRMSEDLSIYLNNAGIKARYMHSDIDSIERAKIIRDFRLGEFDVIVGINLLREGL
                     DLPEVSLVAILDADKTGFLRSTRSLIQISGRAARNVDGKVIFYADEITEAIKQTVAET
                     NRRRAKQLAYNEEHGITPQTIYKTIDQIMQSTAIAEGYDKIAKQATFEKPSAKEFMEY
                     LELDNKDKIIELLTKEMNKAAANLDFEHAAELRDKLWELQQNI"
     gene            complement(4284..4919)
                     /gene="udk"
                     /locus_tag="PLG69_05150"
     CDS             complement(4284..4919)
                     /gene="udk"
                     /locus_tag="PLG69_05150"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPI42931.1"
                     /translation="MSQTNNVRLILIGGGTCSGKSTIAKAIGMRLNDLKTVIVAQDNY
                     YKDLSHLTPAMRAKVNFDHPEAIDIPYLLSDLKLMLEGKAVDIPDYDFATHCRKEGKT
                     CVAFADVIILEGIFGLYYPELLALSDLKIYVDTDSDLRLARRMQRDIVERGRDVESVL
                     EQYLSTVKPSHEAFIEPTKKNADIIIPGDREFDNVLYMLNGYLLYEFIMER"
     gene            complement(4903..6138)
                     /locus_tag="PLG69_05155"
     CDS             complement(4903..6138)
                     /locus_tag="PLG69_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42932.1"
                     /translation="MCAGLDRVGSIKNSERKKENMVMLTKKVLFIDEPFTAEEGIRAE
                     RSRFLWNVISKSFDADLLLLKSPVYMEKPVSLHTGYDKLYSLSLRDENNLESESYHLI
                     GKGQKERFINILDSKRYEVIFFAGLACLPLVCLAKKILPQCKLIIDIEKVWLPEIKEK
                     WKNNKQLKNANYLWQYIRQTAWNKYLPKKGIFCLYANPGETDKLFKCYHFKPENTHFV
                     PLPITTIPELDKSGSNKKFILFWGAPESKDNLTAVKNIISEIYPRISKKMVEKELSIV
                     LCGGEELQGLCSGRITYAPYSEKEQLLQEALLVILPLNKPDTEGRILTCAQFRKTLVC
                     TLSAIKNFPLPEDSYLASDNFAELSEKIIELFRSAKKLDEYANKLSTFCFANFTPEKV
                     ENALLTKINSWIGNNVSNQ"
     gene            6547..6819
                     /locus_tag="PLG69_05160"
     CDS             6547..6819
                     /locus_tag="PLG69_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPI42933.1"
                     /translation="MKIRPVEDHLAVKLSTTESEKTVGGIIIPDTAKEKPQIAEVIAV
                     GNDEDLQKIVKVGDKVLFGKYAGTEIELEGEKLLILAKSDLLAIVE"
     gene            6824..7147
                     /gene="trxA"
                     /locus_tag="PLG69_05165"
     CDS             6824..7147
                     /gene="trxA"
                     /locus_tag="PLG69_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPI42934.1"
                     /translation="MAILEVNSANFETEVLKSTQPVLVDFWAPWCGPCRALSPTIEKI
                     ANETEGKVKVVKINVDEAQDIAAKYSVMSIPTLLIFVNGVVSDQLIGLVQKDKIMDKL
                     NKFIA"
     gene            complement(7424..>10725)
                     /locus_tag="PLG69_05170"
     CDS             complement(7424..>10725)
                     /locus_tag="PLG69_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPI42935.1"
                     /translation="NPEFWDFGELIMGLTSSKVFTITNTGGATLYINNVYTTNSSYSI
                     LQQPGNCELSFNESTTFTVLFNPLEGGTQTGTVVICYNGGEKTIALTGSCIDTTINSF
                     PYTQSFENGGENWLVSAGVGATYYWELVRNDTAHGAVSAYDGSYFARLNVYSAHYDYN
                     PYCLMSPPMNLSTGNKRLNYWVWIGANGYPAPLDVQISTDNQATWTTLFSHDLSVTNT
                     WFNNIISLSSYSTSHIFIRFKAISNYGNGYCNLGLDKITIEDVPPTPIMSYTPGEIIF
                     PYTNVKSVTDYINVTITNIGGGILSLSESSFTLQGNNPNDFEINNFNLPANLGTGQRL
                     NIPVRFKPVAEGNRTAILRIDDGLRSGYDVSLTGYAAGQYVLCENFEGTFPAEDWTSP
                     STSWIRNSDFSHTGTASALAGYTAGTYWLVTPKLRPIEGANTLTFWYRDYSSDTSWDY
                     NNEYTYVLVSKSTDFSAATTLWTGNYQTFTTSWQQASISLSAYNEKDIYIAFKHIATG
                     GNYRMIDDITGVHLSFSTVPNPAVIVSPANEAEDVSLTQTLNWTSGGGSPRGYYLSFG
                     SVIPPLIYLDNVDMGIITSYTPNLDINKRYWWKVVPYNEAGNAENCPIWTFTTMEDPT
                     IRTFPYTEDFEGETFLPLGWTKIVHNGNDITKDATKNHSNPGQYSVRFSSYNLSDNYN
                     QYLFTAPIFVRAPYTKLTFWHIKENASDETLEWGISTNPNPSSFTWNPVNLSSSEWQR
                     TEINLRSYMGHKIYIGFHYYGNYSYYVYLDDINIKATIPANVPTPIEDIVINPTIDLD
                     LDESVDETNPIVQSLPNITALNNLVVLGLIGTETANMTFEVGPGTWYGICYYGGEWHH
                     SIPYPCTVAEGATGTITFENVDFGAKGEIIVVMNEGQNPTLPVELSAFTVNLNPQSGI
                     NIMWVTQSETGVNGYYIYRANVDTLSLASLISPLIRASNTSQQQVYLYTDKEIYESGI
                     YYYWLETQDIDGVSIFYGSLSIDYKGNNNGTPEIPLVTGIRSIYPNPFNPSTTISYEL
                     TKTAEVKIEIYNIRGQLVRSYALGKKERGRYKLLWEGDDNNRRSCGTGMYFIRMQADK
                     ENFIKKVTLLK"
BASE COUNT         3325 a         2065 c         1925 g         3410 t
ORIGIN      
        1 cctgggaagt tttggcttac aattttcacc tgccttcaat gaatacacaa cccactaact
       61 attacactta caaccgagct tgcactgatt ttcgaattaa aaaattgtga gccaaaataa
      121 aaagcccgct ccctttcaac cgcattacaa ctaatccaca aattacacag taacgaagaa
      181 caactccaga atgcgaaaaa aagaaatagc aagttccatc atcgcaaaat ctatattcct
      241 tcgttaccgt tagcattgag gggaggagat agctcggtgc aagtgtgcag ggttcttgtt
      301 ttaactcaca attttgaaat gaagagacca gttataattt ggttgtaagt gtggataaat
      361 tgagggtttt ggtttctgtg agggcagtgg caaaattgta agtcaaaact ttctcaccgg
      421 tgaaaattgt aagtcaaaat tttctccaac cctctcacta cctcactttt cggtgttctc
      481 ggtgttaaat attattccta tttctctttt tgcactatca atggagtcag aagcatgaac
      541 tgcattttcg gtaattcctt cggcataaag atagcggata gttccctgtt ttcttttttc
      601 gggatttaca tctccgatta aatctcgcaa ttcctgaaca gcgttatctt tctggacaat
      661 gattgcaaca gtgaaatccg acatcataaa gttcaacaat ttttcaaaga agctctttcc
      721 tttgtgttcc gcataaaaat gctccgctag ttctctatta aaacggaaaa tcttaagcgc
      781 gcaaaaatga aaattatttt cttccagaat ggtgataata tgccctatat gtttattgcg
      841 aactgcattg ggtttaatta gcagaagtga tttttccttc attttgccgt ataaacggtt
      901 tcaataaaat ccagagcggc aactttgata gcaatatcac ggtttagaac catactcatt
      961 ttgattttgt gctttaggat atcagtccaa atttcataca tctcattggg cttaaattca
     1021 attccggtgt aaagtttaag catatcctgg tagaaatcat cccggtcaga atcaaccctg
     1081 gcaattacca gtttaggtgt ttcctgacaa atatcgtatt ccttataacg gatatccaca
     1141 actacatagc ccaactttag caggttttca tagttaatct gtaagtcctc atggtttagc
     1201 atttttttta actcccaagt taaataattc tggacaaaac acaggattct gaatccttgt
     1261 aaagcaagga agaaatcaag ccctgctctg tcaagaagaa tttattgccc gcaaaggagc
     1321 ccaaaaaatg caacacttca agcttataag tgattatact ccttcaggtg atcaacccga
     1381 agccatagaa gcccttatta aaggtattga agaaggcaaa aggcatcagg ttcttttagg
     1441 cgttacgggt agtgggaaaa cctttaccat agctaatgtg attgctcatt ttaatcgtcc
     1501 tacccttgtt ttatcgcata ataaaaccct cgcggctcaa ctttatggtg aattgaaaca
     1561 actctttccg gaaaatgcgg tagaatattt tatcagttat tacgattact atcagcccga
     1621 ggcatacatt cccggtaagg atatttacat tgaaaaagat agcagtatca atgaacagat
     1681 agaaaaactg cgtttgcgtg ctacaatgag tttgatggaa agaagggatg taattatcgt
     1741 ttcgtctgtt tcctgcattt atggacttgg tgttccggaa gaatatcgtg aggcattaat
     1801 ttccctaaaa aaaggtatga aaatggaccg tgaagaactt attcatcaac tgatatcggc
     1861 tcattacagc agaaatgata ttgcttttga aagaggtgct ttcagggtta gaggcgatgt
     1921 agttgatatt tttcctgcct atttggaaca ctgtttgcgc gtggaattct ttggggatga
     1981 gatttatcgg ctggaaaaaa ttcatcctat ttcctgtcaa cccttggaag aagtagaaaa
     2041 ttacgatgtt tatcccgcaa ttcacttttt aatgaatgaa gaaaaattgc gcaaagccct
     2101 catcagtatt gagcaggaaa tgaatgaacg ggttgcctgg tttttacaaa atcaaaaata
     2161 tgtggaagca gaacgcctga aacaacgcac aacttttgac ctggaaatgc tccgcgaact
     2221 gggttattgt agtggcattg aaaattacac gcgtcactta acgggagcta aacccggaga
     2281 accgccaaac tgtttactgg attactttcc tgaggatttt ttaatggtga ttgatgaatc
     2341 ccatgtaact attcctcaag tgcatggaat gtttgccggg gactacactc gcaaaaagaa
     2401 cctggtagaa tacggttttc gtttaccttc tgcttttgat aaccgtcctt tgcgttttga
     2461 agaatttgaa aagtttatgc atcaggtaat cttcgtttct gccactcctg cggattacga
     2521 actaagttta actaaaggcg aaattgtaga acaggtgatt cgtcccaccg gtttgcttga
     2581 cccggaaatt gaaattaaac ctataaaaca tcaggtagat gacctcatcg cgcaaatcaa
     2641 agaacgggtt gctaaaaaac aaaagatatt ggttatgaca ctaactaaaa gaatgagtga
     2701 agaccttagc atttatctga ataatgccgg aatcaaagcc cgctatatgc acagtgatat
     2761 agacagcatt gaaagagcta aaatcatcag ggactttcgt ttaggtgaat ttgatgtaat
     2821 tgtaggcatt aatcttttaa gagaaggttt agacctgcca gaggtttcac tggtagctat
     2881 tttagatgcc gataaaaccg gtttcttgcg ttctacccgt tccctgattc aaatttccgg
     2941 tagagcagcc agaaatgttg atggcaaagt gattttttat gctgacgaaa ttaccgaggc
     3001 cattaaacaa acagttgccg aaaccaatag acgccgtgct aaacagcttg cctacaatga
     3061 agaacacgga atcactcctc agaccatcta taaaacaatt gaccagatta tgcaatccac
     3121 cgctattgct gagggttatg ataaaattgc caaacaagct acatttgaaa aaccctccgc
     3181 taaggaattt atggaatatc tggaactgga taataaagat aaaatcattg aactgctcac
     3241 taaagaaatg aataaagccg ctgctaacct tgattttgaa cacgctgctg aactgagaga
     3301 taaactttgg gaactgcaac aaaacatata aaaaatgaag aatgatagca cgcagatttt
     3361 atttttacaa agagaaaaga gagaaagaga ataatttaga atgtaaaatg aatttgactg
     3421 ttcttgtttt aactcacaat ttttggtttg gaaaccattt gtagttcggt tgacattgtg
     3481 cttgcttctt gttttaactc acaatttttg gttgggaaac catttgtagt gcggttggaa
     3541 gtgtagaagc aagagggatg ctgataattg ggagaacagg tgaaaattgt aagtcaaaac
     3601 aatcatcgtt gcaactatat tttgccgcgg tggagttttg actctaaacc gattctttta
     3661 tgtttgcaac tatattctga ggtaacagga aattttgttt gttgccttct actttcaaag
     3721 ctgaagctcg gtttagagtt aaaacgaaag tattccccga agtcacaaat tctgaacaat
     3781 tcctacccca catttctgga gcggctgaat atatattttt tccgtgaccg tagaattcgt
     3841 gagtttgttg tagctcggtg caagtgtgct tgcttcttgt tttaactcac aatttttagg
     3901 ttgggaaacc atttatagtg cagttggaag tgtagaagca agagggatgg tgataattgg
     3961 gtaaacgaat gaaaattgtg agtcaaaact tcccaggggt gcttcgcaaa ttttacgagg
     4021 ggcttacgcc accatcgcta tcattgtgtc gcttttgggc ttttatggaa tctaaaccct
     4081 ctcaaccttc taaaccatct caaccattaa acttctacaa accctctcaa cccttctcaa
     4141 cctcttttaa attcgttcta cattttacat tttacactct acattttaca ttctcaatta
     4201 ttctcttttt ctcattattc tcttcttctc tttttctctt tgtaaagaaa ctcaacttta
     4261 ccctgcactt catttccatc tcgctatctc tccattataa actcatacag caaatatcca
     4321 ttcaacatat acagcacatt atcaaattct ctgtctccag gaataataat atctgcattc
     4381 ttcttagtgg gctcaataaa tgcctcatgg gagggtttaa cagtgcttaa atattgttcc
     4441 aaaacgcttt ccacatctct tccccgctcc acaatatctc tttgcatccg cctggctaaa
     4501 cgtaaatccg aatcggtatc cacataaatc ttgaggtcgg aaagagccaa aagctcagga
     4561 taatacagcc cgaagatacc ttccagaata attacatccg caaaggcaac acaggtcttt
     4621 ccttcctttc tgcaatgagt ggcaaaatca taatccggaa tatccactgc cttaccttcc
     4681 agcataagtt tcaggtcaga aagcagatag gggatgtcaa ttgcctccgg atggtcaaaa
     4741 tttactttag cacgcattgc aggagttaga tggctaaggt ctttgtaata gttatcctgg
     4801 gccactataa cggtttttaa gtcatttaat cgcattccga ttgccttggc aatagtgctc
     4861 tttccggaac aggttccgcc tccgataagg attaaacgaa cattattggt ttgagacatt
     4921 atttcctatc cagctgttta ttttagtcaa taaagcattt tccacttttt ccggcgtaaa
     4981 attagcaaag cagaaagtgc ttagtttatt tgcatattca tccaatttct tcgcactgcg
     5041 gaaaagttct attatttttt ccgataattc ggcaaagtta tctgatgcaa gatagctatc
     5101 ttccggtaaa gggaagtttt ttattgcgga taaagtgcaa accagggttt tacgaaattg
     5161 agcacaggtt aaaatccttc cttccgtatc tggtttattt aaaggcaaaa taaccagcaa
     5221 tgcctcctgc aataattgct ctttttcgct gtaaggagca taagttattc ttccgctgca
     5281 aagtccttga agttcctctc ccccacacag aacaatgctt aattcctttt ctaccatctt
     5341 tttggatata cggggataaa tctcggaaat aatattcttt actgcagtaa gattatcttt
     5401 actttcagga gcaccccaaa aaaggataaa tttcttgttg cttcccgatt tatcaagttc
     5461 tggtatagta gttatcggta aagggacaaa gtgtgtattc tctggtttaa aatgataaca
     5521 tttaaagagc ttatcggttt ctccaggatt agcgtataaa caaaatatac ctttcttcgg
     5581 aagatacttg ttccaagcag tttgcctaat atattgccaa agataattag cgtttttcag
     5641 ttgtttatta tttttccact tttcttttat ttccggtaac caaacctttt caatatctat
     5701 gattaattta cattggggta aaatcttttt tgctaagcaa actaatggca gacacgcaag
     5761 tcctgcaaaa aagattactt catagcgttt gctgtccaga atattgataa aacgctcttt
     5821 ctgacctttt cctataagat gataagattc agattcaaga ttattctcat ctctcaagct
     5881 taaggaatat aatttgtcat aacccgtatg caaagaaacc ggtttttcca tataaacagg
     5941 acttttcagc agaagaaggt cagcatcaaa acttttggaa ataacattcc acaaaaaacg
     6001 cgaacgctca gctctgatcc cttcttctgc agtaaagggc tcatcaataa acaatacttt
     6061 tttagtaagc ataaccatat tttccttctt gcgttcacta tttttgatac tgccaactct
     6121 gtcaagccct gcgcacatct gattattttt ctttcacgca gatttcgctg attccgcaga
     6181 taaaaaagat aataggtctg ggatgaacaa gattaaaggg atttaagaga tttataatat
     6241 agaatgggtc ttgataaaat aacaagacac cttctatttt ttccttttat tccacaccaa
     6301 ttctatctta tttatgcatt ctatattaaa tcactaattc tcttatctct ttaaaaaatc
     6361 tgcgtaatct gcgtgctgac attttcattc tcaattattc tcttttcctc tttttctctt
     6421 tgtaaaccat aatctgcgtg ctctcatttt tcccttgaca gaaattagca gtccattttt
     6481 gtgtttgcta agtaagccct ttcgcttata ttataattga aaaataaata cattatggag
     6541 gttacaatga aaattagacc cgtagaagac catttagcgg tgaaacttag caccacagaa
     6601 agtgaaaaga ccgttggcgg aataattatt cccgatactg ccaaagaaaa acctcaaatt
     6661 gctgaagtaa tcgcagtcgg caacgatgag gatttgcaaa aaatagtgaa agtgggcgat
     6721 aaagtcctct ttggtaaata tgctggaacc gaaattgagc ttgagggaga aaagctgctt
     6781 atccttgcca aaagcgatct cctggctatc gtagagtgat aaaatggcta tcctggaagt
     6841 taattctgcg aactttgaga ccgaagttct aaagtccacg caacccgttt tggttgattt
     6901 ttgggctccc tggtgcggtc cctgcagagc acttagccct acaatagaaa aaatagctaa
     6961 cgaaaccgaa ggcaaagtaa aagtagtaaa aataaatgta gatgaggcac aggatattgc
     7021 cgctaaatat tccgtgatgt ctattcctac tttactgatt tttgtgaacg gtgttgttag
     7081 cgaccaattg ataggactgg tgcaaaaaga caaaattatg gataaactga ataagtttat
     7141 cgcttaagct atatctgaaa acaaacagcc cgggagttat tggccgggct gtttttctta
     7201 tctttcaaag atttcactga taacatagat tttttattct ttgaattccc tttacataag
     7261 atcctgctga tccaatcaat ccagttatct gcgttctgtt tttttctccg tcacgactcc
     7321 tgacttaatt ctttcaccgt tcactattca catttcattc tcaattcgca gttaaatctc
     7381 tttttctctt tatctctttg taaaaaatct atctcaccat tcttcatttc aggagagtta
     7441 ctttctttat aaagttttcc ttatcagctt gcattctgat gaaatacatt cctgttccac
     7501 aggagcgtct attattatca tcaccttccc aaagcagttt atacctacct ctttcttttt
     7561 tacctaatgc ataagaacgg actaattgtc cgcggatatt atagatttca atttttactt
     7621 ctgcagtttt agtaagttca tagctaatag tagtggaagg attgaaaggg tttggataaa
     7681 tagagcggat tcccgtcact aaaggtattt ccggagtgcc gttattattt cctttataat
     7741 caatactaag tgaaccgtaa aaaatggaaa caccatctat atcttgtgtt tccagccagt
     7801 aataataaat tcccgattca taaatttctt tgtccgtata aagatatacc tgctgttgag
     7861 aagtattaga agctctaatt agagggctaa tcaagcttgc cagggaaagt gtatcaacat
     7921 tagctctgta gatataatat ccattcacac ctgtttccga ttgggtaacc cacataatat
     7981 taattccgct ttgaggatta aggtttaccg taaaagcgga taattctaca ggcaaagtag
     8041 gattttgacc ttcattcata actacaatta tttcgccctt tgctccaaaa tctacatttt
     8101 caaaagttat agttccagta gctccttctg caacagtgca aggataggga attgaatgat
     8161 gccattctcc tccataataa caaattccat accaggttcc aggacccact tcaaatgtca
     8221 tattggctgt ttctgttcca attaaaccaa gcacaacgag attattaagt gcagtaatgt
     8281 tgggtaaaga ttgcacgatt ggatttgttt catccacact ttcatcaagg tctaaatcta
     8341 tggtgggatt gatcacaata tcctctatag gagtagggac attagcagga atagtagctt
     8401 ttatattgat atcatccaga taaacataat aactgtaatt gccataataa tggaacccta
     8461 tataaatttt atgtcccata taacttctta aatttatttc agttctttgc cattcggaac
     8521 tgcttaaatt taccggattc caagtaaaac tggaaggatt aggattagtg gatatacccc
     8581 attccaatgt ttcatcactg gcattttctt ttatatgcca aaaagtaagt ttagtatatg
     8641 gagctctaac aaagataggt gcagtaaata gatattgatt gtaattatca gataaattgt
     8701 aggaactgaa ccgaaccgaa tactgaccgg gattggaatg atttttagtt gcatccttag
     8761 taatgtcatt tccattatga actattttag tccaaccaag aggaagaaaa gtctctccct
     8821 caaaatcttc agtataagga aaagttctaa tagtaggatc ttccatagtt gtaaaagtcc
     8881 agattggaca attttcggca ttaccagctt cattgtaggg gactaccttc caccaatatc
     8941 ttttgtttat atcaagattg ggtgtatagc tggtaataat tcccatatcc acattatcta
     9001 aataaattaa gggaggaata acacttccaa aggatagata ataacctctg ggtgaaccac
     9061 ctccacttgt ccaattcaat gtttgggtaa gagaaacatc ttcagcttca ttagcaggac
     9121 ttactattac tgccggattg ggaactgtag aaaaagataa atgcacaccg gtaatatcat
     9181 ctatcatccg gtagttgcca cctgttgcaa tgtgtttgaa agcaatgtaa atgtcctttt
     9241 cgttataggc acttaaggat atagatgcct gttgccagga agttgtaaag gtctgataat
     9301 taccagtcca aagagtagtg gcagctgaaa aatccgtgct tttagaaacc agtacataag
     9361 tatattcatt attataatcc caagatgtat ctgaagaata gtcccgatac caaaaagtta
     9421 aagtattcgc tccttctatt ggtcttaatt taggtgttac caaccaatat gttccggcag
     9481 tataaccagc taaagctgaa gcagttccgg tatgagaaaa gtcggaattt cttatccatg
     9541 atgtggaagg tgaagtccag tcctctgcag gaaatgttcc ttcaaaattt tcacaaagga
     9601 catattgtcc cgctgcataa ccagttagag agacatcata tccggatcga agtccatcat
     9661 caattcttaa aatagctgtt ctattgcctt ccgcaactgg cttaaaacga actggaatat
     9721 ttaacctttg tcctgtgccg agatttgcgg gaagattaaa attgtttatc tcaaaatcat
     9781 tgggattatt gccctgcaag gtaaaactgg attcacttaa gcttaatatt cctccaccta
     9841 tattagttat tgtaacattt atataatcgg taacactttt tacattggta taaggaaaaa
     9901 taatttcccc gggtgtgtaa ctcataatag gagttggagg tacatcttca attgttattt
     9961 tatctaatcc caaattacaa tatccatttc cataattgct tattgcctta aaccttataa
    10021 aaatatggga ggtagaatag cttgataaag aaatgatatt attgaaccag gtgttagtaa
    10081 ctgacaaatc gtgactgaac aaagtagtcc aggttgcttg attatcagtt gaaatttgca
    10141 catccaaagg agccggatag ccgttagctc caatccagac ccaataattc aatcttttgt
    10201 tacctgtgct taagttcata ggaggagaca taagacaata aggattataa tcataatgag
    10261 ctgaataaac atttaagcgt gcaaagtagg aaccgtcata agcagaaaca gcaccgtgag
    10321 ctgtatcgtt tcgaactaat tcccaataat atgttgctcc cactcctgca gaaactaacc
    10381 agttttctcc tccattttca aaactctgag tgtaaggaaa agaattgatg gtagtatcta
    10441 tacagcttcc agttaaagca attgtttttt ctcctccatt ataacatata accacagttc
    10501 ctgtttgagt tcctccttcc aagggattaa agagaacagt gaaagtagtt gactcattga
    10561 aagaaagttc acaattgcct ggttgttgaa gtatagaata tgagctgttt gtagtataca
    10621 cattatttat atataaagta gctccaccgg tattagtaat tgtaaatact ttagaggagg
    10681 ttaaacccat aattagttct ccaaaatccc aaaattcagg attca
//
LOCUS       DAOPFL010000057        10657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_7588_length_10657_cov_69.316072, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000057 DAOPFL010000000
VERSION     DAOPFL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7588_length_10657_cov_69.316072"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..4962
                     /locus_tag="PLG69_05175"
     CDS             391..4962
                     /locus_tag="PLG69_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI42936.1"
                     /translation="MKKSLFLIAIILISWVSLLAIPQPVHIVPVSTDKVNSFGVDCFH
                     LQGSVAYCVDNTKGIIKAWDASKKEFLQPIFTKLPYASKADDITGDETTLYILDSKAS
                     SIYLYGYDGNYLRTISFKGSPAVQFKKAIRILVNYQGFIYVLDAGRNELLSFTNEGMF
                     TGKISVMAPISMCLGEDQVIHILINKGRNQAMLHFDHNLKPCGSFTFSTPDSKVDKIA
                     DSAINQYNEYYVIYAVSTKIGKTDASGRLFPRSTWGSKDTNVSLVSFQQPTIIKTVDN
                     NGKVLLGILDSKVRTLKLYLDSEFSTMNVLQKPPFTIRPKLLENTAPKAKDYLVQDGR
                     KYYIHTTTVQYNKAKKTTDAITCKDDNGNIFNIYAITEGKRGVKGYDAIAVFNNKLYA
                     VDSKSCKVFIYNALTGDYMDSFGGKGNQNGRLNMPVSIAISSDGLIYIADRDNFRIAI
                     FNENSAHMLNLDFKSKNLKPQLLRISGQTLYFLANNSAIYKMSLDDDKNLKLLVSANR
                     ISTFDVLYENRIGYIDGLTQQLNIIYNNNTEHQYFTKNANGIFPGFAEIELIRYNPTD
                     NNLYICDKMAVNTRLLTFYFSPKKPQTIHFIVNQNKQAELSWDAAEGISNWLVIENNG
                     IQSVTYKVSEPRFVITEPKTQINTYKVQSISADGKSGPPSEEIEDAYSYYRYLSQNKN
                     YKDAAVALDRASKIFSGVNFKEELVQNYTLEAQMYISRNEFEKALSSIDSIEKITGVL
                     IDTAIWKAEIYRMMGDYKQGIAYLEKFKRNDDQNIMRELIALYYMDKNYAKVKELCNS
                     YLANFNRDKEVIRYLALAEEKLQNYANALSAMRELIAVEDNLDNNIKLGELQMLNKEY
                     ETAINSLLRTLQRFNNQGADVIQKLLGDCYFASGNYASAIDKYIDAIQLNPNEAEFYY
                     CLGNAYAKNRKANEATKNYAIAYQKMPAEVKYGFAYAQALEKELHSKEALVVMDNIYQ
                     YIASDSTTTAYHEFYHDLLIKEQRYDDAWKEIQIALKYAPDNKLLQEKARTTSETRKY
                     YNETRDEIEIKKLEFYKVFPALCEYYKTNPIGTVTLYNTREISIENISVTVTIPQITD
                     RPYKKIIPALMGGEELTVDIIAPINNRIFDICKNGSATFNADLEVEWIFNKKQGAAYK
                     SAIIQAQGINAMDWKDRKQYACFINPEDVNLRTFVNTHITQLFKTQPVGELNKNIQRA
                     VQVWCFYSANGIRYIPDMSTANLTGSEIDNVQFPFQTLTQKAGDCDDLLALLAATLSV
                     IGVECGFIDIPGHVMLVINTGITTEEIFSLGFEPSQFIYKNKKYWLPIETTLLGKETF
                     TASWKKASKDYNMLIEKGIDPQLIEFDIAHQLYPPAPYTEQISSYEYGNKTQAITKYE
                     TEIENIKLMGKVAQEEKFVETLKKYPTNLKVANQYALWCVKNNRPGKAAELWYQILTQ
                     DPQNLGALINLGNLQFNGGNYNEARINYLNALELSNDKDPILRNLCILEYKSGNQSQA
                     REYFNLMSNKNLLRDVNPTIYSDLLYIGE"
     gene            4967..5638
                     /locus_tag="PLG69_05180"
     CDS             4967..5638
                     /locus_tag="PLG69_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42937.1"
                     /translation="MKKTMLIITFAGIALCLFSASVGKIRFLVGEVQYKQSLNLTYKP
                     ATLNMEVEPEGYIKTGPDSKAEIQWNNGSVAVINANTQISISKLQSDALANPNYTNKL
                     RERINNLRAQNEQREASQVAGIRREEAEIIPESELYWYSEPKYKVKDALNYMDIRDYT
                     KAVEIFEKVIEQSPLDKDAELSHTCLIMIYDELGDKVNLQKHIKKLKEDFPDSSALDS
                     LPPEK"
     gene            5653..7833
                     /locus_tag="PLG69_05185"
     CDS             5653..7833
                     /locus_tag="PLG69_05185"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPI42938.1"
                     /translation="MSRLRYFLIAIAVLLISILLPELPVISILLRTLDLSIYDTILEV
                     HNAITYSEYKGVYDQICIVDIDEKSIKELGQFSSWPSLFFADLVNTLSADEPVAIAFD
                     VFFTESDSIQGYARERLRPEIEGLTANPDIIMDALSTDDSFAAAIENAGMVYLSMFNS
                     DYPSYTGALPDKLTAWKVQPKHFLQLKYPIPPIPQYASSARGIGFANIEPDVSGKIHD
                     YPLFLQYNNNAYLNFSMQICLDLLGVDQIKVNRNCQLLSGEKIVSELPLSPDGLFYFK
                     YYGQGGPDKTFRYISFSDVLFHRIPPGYFKDRLILVGTSAYGLKDSKITPLDNSFPGV
                     ELHSTFIRNVLEENYVRWVDSRILLIINILALLFMAIFIPRSKPFVSISVFLFISLLS
                     IPAVYLLYVKESYIYNYNYTLVPWVLGFLAMFITQAHEQGKEKRMVRDAFEHYVSPEV
                     IQEIMKEPEKLKAGGEKKYISIMYVDVRNFTSFCEQLTPSEITGFMNNYFNRATQTII
                     AHRGTLDKYIGDAILALFGAPVTYPGFEENAVKSALAIRQIVLNIKEEYKDHPVLKNF
                     RIGIGIATGEVIVGNIGSDRIFNYTGIGDKMNFGSRLEGLNKYYLTTIIIDEYTYRAV
                     SNTVFCRKLDKVKVKGKLNPCEIYEIIDTHENMQNQPELISAYHKYETALDLMLAGQK
                     NQAKTLFEEVLAILPSDEPSRIMLNRCEIIDWDTWDGSWQFESK"
     gene            8522..9340
                     /locus_tag="PLG69_05190"
     CDS             8522..9340
                     /locus_tag="PLG69_05190"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPI42939.1"
                     /translation="MRNLLLQEPVLVVDIGNTNIVCAIYLKGKSIWTVSLKSSRENTS
                     DEYYVLLGSLLESTTLPDGYKLGLKDIRYIVLGSVVPELTRVWKHLCSKYIQAEVYEI
                     NALSPLGISFRVDNPSFIGADLIANAYAAWQKYRTSSIIIDLGTATTIELISPEGMFE
                     GAIIAPGIKIGAESLFSKAAKLYEIELVSPTALLGLNTTEAMLSGIIYGHSFMLDTFI
                     AKIKEQYSQLKPFQTILTGGMSSLIKPYLSEINTIDKGLTLDGFYLALGKILNQ"
     gene            9351..>10657
                     /locus_tag="PLG69_05195"
     CDS             9351..>10657
                     /locus_tag="PLG69_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42940.1"
                     /translation="MTKKLFACLILLALTILWATETVPEGANPPEDDQTEQKGIFPAR
                     YFRPKKEIKHELDPLTEKALYPRLYLTEYNPIINNRIEVYKINKGMNYYKTKPYEIPA
                     FAPEYKEEIDFANSKVILSVQVGNYKIAPDVPISFDRYFSNMQTKVFHKSLIANFKTQ
                     EQVVQTVTSGLFKDLTLLPEIAMPKAMQKVLGSSAGRLNLDGTQKLTMQVSNTHRKQV
                     PIYDQSGKNVFDLKMEMETNLRLSGTIGDKIAVNFKYNSKQDEELFDMNNVNVKYTGY
                     DDEFIQSIEGGNIALSLSGSRYVSYSASSQGLFGITSKFKYGNLDLSVIASKEESQKN
                     TQTYIGKSQADSSTVSSWKYARRTMYYLHDPYQLYQLKTGTNIPPGWINNAIETAPDG
                     SWMVNVNFLPANGTVRLFYDDANYTNNTASAVGDTIYYSNNMRP"
BASE COUNT         3580 a         1743 c         2024 g         3310 t
ORIGIN      
        1 catataagac agaaaaaatg tttatgcatt ttaattcatc ggtattattg ggattgaaga
       61 caagtttaaa atgcttacaa atatacttaa gaaatgcaat taggagttat ccgctaaaag
      121 gtgctttttt ttacaattaa cttcgttcca aatatattgt taaaaaaaat ggagctttta
      181 caggataatt ttttccttga cagaataaga taatttgaag taaaagaaaa aagataaaag
      241 tggaggatat aaagttgaag aactggttat tatctctgcg taaaacaaat ggatttgtcg
      301 gcttaaagcc ctcttgaaaa aggaggtttt taagatttga tgctgtgtga agataacata
      361 atttagatta tgaaaaaata gggagtaaat atgaaaaaat cccttttcct gattgcaatt
      421 atcttgatta gttgggtatc gctattagca attcctcagc cggtgcatat agttcctgtt
      481 tctacagata aggttaattc ctttggagtt gattgttttc atcttcaggg tagtgttgca
      541 tattgtgtag ataatacaaa aggtataatt aaggcatggg atgcaagcaa gaaagaattt
      601 ttgcaaccga tatttaccaa acttccttat gccagcaagg cggatgatat tacaggagat
      661 gaaacaactt tatatattct ggattccaaa gccagctcaa tttatttata tggatacgac
      721 ggcaactatt taagaactat atcttttaag ggttcaccag ctgttcagtt taaaaaagct
      781 attcgcattc tggtaaatta tcagggcttt atttatgttt tggatgcagg acgaaatgaa
      841 ttgctttctt ttaccaatga aggtatgttt acgggtaaaa tttcggttat ggcacctata
      901 tctatgtgtt tgggagaaga ccaggtaatt catattctga taaacaaagg tagaaatcag
      961 gcaatgctgc attttgatca taatcttaaa ccctgcggta gctttacatt ttctactcca
     1021 gatagcaaag tagataaaat tgccgattct gccattaatc aatataatga gtattatgtt
     1081 atttatgctg tgagtaccaa gattggcaag acagatgctt ccggacgcct atttccccga
     1141 tcaacctggg gttcaaaaga tactaatgta tctctggttt cttttcaaca gccaactatt
     1201 atcaaaacag tagataacaa tggaaaagtt cttttaggga tattggacag caaagtccga
     1261 actcttaaac tctatctgga tagcgagttt tctacaatga atgtgttgca aaaacctcct
     1321 tttacaatac gacccaaatt gctggaaaat actgcaccga aagccaagga ttatcttgtt
     1381 caagacggca gaaaatatta tattcataca accacagtgc agtataataa agctaaaaag
     1441 accaccgatg ccattacctg taaagatgat aacggcaaca tttttaatat ttatgctata
     1501 actgaaggga aaagaggagt gaaaggttac gatgctattg ccgtctttaa taataaattg
     1561 tatgcggtag atagtaaatc ctgcaaggtt tttatttata atgcattaac gggtgattat
     1621 atggattcct ttggcggaaa aggaaatcag aatggacgat taaatatgcc tgttagtata
     1681 gccatttctt ccgatggttt aatttatatt gcagacaggg ataattttcg gattgctatc
     1741 tttaatgaaa attctgctca tatgcttaat ttagatttta agagcaaaaa tttaaaacct
     1801 caattattgc ggattagtgg tcaaacacta tattttctgg caaataattc ggccatttat
     1861 aagatgtccc tggatgatga caaaaactta aaactattag tttcagctaa tagaataagc
     1921 acttttgatg ttttatacga aaacaggatt ggctatattg acggattaac acagcagttg
     1981 aacattattt ataataacaa tacagaacat cagtacttta ccaaaaatgc taacggtatt
     2041 ttccccggtt ttgcagaaat tgaattaatc cgttataatc caactgataa taatctttac
     2101 atttgtgata aaatggctgt caatacacga ctacttactt tttacttcag tcccaagaaa
     2161 ccccagacca tacattttat agtaaatcag aataaacagg cagaactttc ctgggatgca
     2221 gcagaaggga tatcaaattg gttagtaatt gaaaacaatg gaattcaaag tgtaacatat
     2281 aaagttagtg agccgcgttt tgtaattacc gaaccgaaaa cacaaataaa tacctataaa
     2341 gtgcagtcaa tttctgctga tggaaaaagc ggtccgcctt cggaagaaat tgaagatgct
     2401 tattcttatt accgttattt atctcagaat aagaattata aagatgcagc agtagcattg
     2461 gatagagctt ctaaaatctt ttcaggtgtt aattttaaag aagaattagt ccagaattat
     2521 actttggaag ctcaaatgta tatcagtcgg aacgaatttg aaaaggcatt atccagcatt
     2581 gactctatag aaaaaattac gggagtgctg atagatactg ccatctggaa agctgaaata
     2641 tacagaatga tgggggatta taagcagggt attgcttacc tggaaaagtt taagagaaac
     2701 gatgaccaaa atattatgcg tgagctgatt gcattgtatt atatggataa aaactatgct
     2761 aaagtgaagg aactctgtaa ttcctattta gctaacttta accgcgataa agaagtaatc
     2821 cgatatcttg ccttagctga agaaaaactg cagaattatg ccaatgcttt atctgcaatg
     2881 cgggaattga ttgctgtgga agataatctg gataataata ttaaactggg tgaactgcaa
     2941 atgttgaata aagaatatga gaccgcaatc aattctttat taagaacttt gcaacgattc
     3001 aataaccagg gtgcggatgt tattcaaaaa cttcttggcg attgctattt tgcatccgga
     3061 aattatgcga gtgccattga taaatatata gatgccattc agctcaatcc taacgaagca
     3121 gaattttatt attgtctggg aaatgcttat gctaaaaatc ggaaagcgaa tgaggcaacg
     3181 aagaattatg ctattgctta tcagaaaatg cccgctgaag taaaatacgg ttttgcctat
     3241 gctcaggcat tagaaaaaga attgcattct aaggaagcgt tagtggtgat ggataatatc
     3301 tatcaatata ttgcttctga tagcaccaca actgcttatc acgaattcta tcatgattta
     3361 ctaattaaag agcaacgcta tgatgatgcc tggaaagaaa ttcaaattgc gcttaaatat
     3421 gcacctgata ataagctttt acaggagaaa gccagaacta cttctgaaac aaggaaatat
     3481 tataacgaaa caagagatga gatagaaatt aagaaacttg aattttacaa ggtctttccc
     3541 gccctctgcg aatattataa aaccaatcct attggaaccg ttacccttta taatacgcgt
     3601 gaaatttcca ttgagaatat ttctgtaact gtaaccattc cccaaataac agatcgtcct
     3661 tataagaaaa ttataccggc tttaatgggt ggtgaagaac ttaccgtgga tattatagct
     3721 cctatcaaca acaggatatt tgatatctgc aaaaatggtt ccgctacttt taatgcggat
     3781 ctggaggttg aatggatatt taacaaaaaa caaggtgctg cttataaaag tgccattatt
     3841 caggcacagg gtatcaatgc tatggactgg aaagatcgta aacagtatgc ttgttttatc
     3901 aatcctgaag atgtaaattt gcgcactttc gtaaatactc atatcactca acttttcaaa
     3961 actcaacccg tgggagaact taataaaaat atccaacgcg cagttcaggt ttggtgtttt
     4021 tatagtgcta atggtatcag atatattccg gatatgtcta cagctaattt gaccggcagt
     4081 gaaatagata atgtccaatt tccttttcag accctaactc aaaaagctgg tgattgtgac
     4141 gatttgcttg ctctattggc tgctacttta tcggtaatag gcgtagaatg cggttttata
     4201 gatattccgg ggcatgttat gctggttatc aataccggaa taactacgga agagattttc
     4261 tctcttggtt ttgaaccaag ccagttcatc tacaaaaata aaaaatactg gcttcctatt
     4321 gaaacaacgc ttttgggtaa agaaaccttt actgccagct ggaaaaaagc cagcaaggat
     4381 tataatatgc taattgaaaa aggaatagac ccacagttaa ttgaatttga tattgcacat
     4441 cagctatatc ctccagcacc atacacagaa caaatttcca gttatgaata cggcaataaa
     4501 acgcaagcaa taactaaata cgaaactgaa attgaaaaca taaaattgat gggaaaggta
     4561 gctcaggaag agaaatttgt ggaaacgctt aaaaaatatc ctaccaatct aaaggttgcc
     4621 aatcaatatg ctctgtggtg tgtgaaaaat aatcgtcccg gaaaagctgc agaattatgg
     4681 tatcaaattt taactcaaga ccctcaaaat cttggtgctt taatcaattt gggcaatctc
     4741 caatttaacg gaggtaacta taacgaagcc agaatcaact atttaaatgc cttagaactt
     4801 agtaatgata aggatccaat attacgcaac ctctgtattc tggaatacaa aagcggaaac
     4861 caaagtcaag ccagggaata ttttaatctg atgagcaata aaaatcttct gcgtgatgtt
     4921 aaccccacaa tatattccga tttgctatat ataggagagt aaaaaaatga agaaaactat
     4981 gcttattatc acttttgcag gaatagcatt atgtcttttt tctgcttctg taggtaagat
     5041 tcgtttttta gtaggtgagg tgcaatataa acaatcacta aatctcacct ataagccagc
     5101 aaccttgaat atggaagtgg aacctgaggg ttatattaaa acaggtcccg attccaaagc
     5161 tgagattcaa tggaacaatg gcagcgttgc ggttattaat gccaacaccc aaatcagcat
     5221 cagtaaactc caatcggatg ctttagccaa tcctaattac acaaataagt taagggaaag
     5281 aatcaataat cttagagctc aaaatgaaca gcgtgaagcc agtcaggttg caggaatccg
     5341 aagagaagaa gctgaaatta ttcctgaaag cgaattatac tggtattctg aaccgaaata
     5401 taaggttaaa gatgccttaa attatatgga tattagagat tatactaaag cagtggagat
     5461 ttttgaaaaa gtaattgaac agtcacctct ggataaggat gcggaattat cccatacttg
     5521 tttgattatg atttatgatg aactgggaga taaagttaat ctgcaaaaac atataaaaaa
     5581 gctcaaggag gattttccgg atagctcggc actggatagt ttgccaccgg aaaaataaaa
     5641 gtaagcgaat tgatgtcccg tttgcgttat ttcctgattg ctatagcagt attgcttatt
     5701 tcaattttat tgcctgaact accagttatc agtattttat taagaacttt ggatttaagt
     5761 atttatgata ctattctgga agttcacaat gctattacct acagtgaata taaaggtgtt
     5821 tatgaccaaa tttgtattgt ggatatagat gaaaaaagca tcaaggaatt aggacagttt
     5881 tcttcctggc ccagtttgtt ctttgccgat ttagttaata ctttatctgc agatgaacct
     5941 gtggctattg cttttgatgt tttctttacg gaaagcgaca gcattcaggg atatgccaga
     6001 gaaagattgc gtcctgaaat tgaaggtctg acggcaaatc cggatataat tatggatgct
     6061 ttaagtactg atgatagttt tgccgcagct atagaaaatg ccggaatggt ttatttatct
     6121 atgttcaaca gtgattatcc ttcttatacg ggagctcttc ccgataaatt aactgcctgg
     6181 aaagttcagc ccaaacattt tttacagtta aaatatccta tacctcccat tcctcaatat
     6241 gcaagttcag caagaggaat tggttttgcc aatattgaac ctgatgtttc cggaaaaatt
     6301 cacgattatc cccttttttt gcaatataac aataatgctt atctgaattt cagcatgcag
     6361 atttgcttag atttattagg tgttgatcaa ataaaggtga accgcaattg tcagctgctt
     6421 tccggagaaa aaattgtgag tgaattgcct cttagtcccg atggtttgtt ttattttaaa
     6481 tattatggac aagggggtcc tgataaaaca tttcgttata tttccttttc cgatgtcctt
     6541 tttcatagaa ttcctcctgg ctattttaaa gaccggctta ttttagtagg gacttctgcc
     6601 tacggattga aggacagtaa aataacacct ttggacaact cctttccagg tgtggaatta
     6661 cattcaacat ttatcaggaa tgtgctggaa gaaaattatg tgcgttgggt ggattcccgt
     6721 attttactaa taattaatat tttggctttg ctctttatgg ctatttttat tcctcgcagt
     6781 aaaccgtttg tatctattag cgttttttta tttatttctc tgctttctat tcctgccgtt
     6841 tatttgcttt atgtgaaaga gagttatata tataattata attacactct tgttccttgg
     6901 gttttgggct ttttagcaat gtttattact caagcacatg agcagggtaa agaaaaaaga
     6961 atggtgcggg atgcttttga gcattatgtt tcaccggaag ttatccagga aattatgaag
     7021 gaaccggaaa aattgaaagc aggtggagaa aagaagtata tcagtattat gtatgttgat
     7081 gtgcgcaatt ttacttcttt ttgcgaacag ctaactcctt ctgaaataac gggatttatg
     7141 aataactatt tcaatcgggc aacacagacc atcattgctc atcgcggaac tctggataaa
     7201 tatataggag atgctatttt agctttgttt ggagcgcccg taacttatcc cggttttgaa
     7261 gaaaatgctg taaaaagcgc tcttgctatt cgccaaattg ttcttaatat caaagaggaa
     7321 tataaagacc atcctgttct gaaaaatttc cgtatcggca ttggtattgc tacaggagaa
     7381 gtaattgtag gaaatattgg ctccgataga attttcaatt ataccggcat tggtgataaa
     7441 atgaatttcg gttcccgact ggaaggattg aataaatatt atcttactac tattattatt
     7501 gatgaataca cttatcgggc agttagcaat accgtctttt gtcgtaaact ggataaggtg
     7561 aaagtgaagg gtaaattaaa tccctgtgaa atttatgaaa ttattgatac acatgaaaat
     7621 atgcaaaatc aaccggagtt aatttctgcg tatcataaat atgaaacggc tttagattta
     7681 atgcttgctg gacaaaaaaa ccaggcaaaa accctttttg aagaggtttt agccatttta
     7741 ccctcggatg aacccagtag aattatgttg aatcgttgcg aaattattga ttgggatact
     7801 tgggatggca gttggcaatt tgaaagtaaa taaaataata agctctcaca gaggacacgg
     7861 atagcacaga tttgattggt aaaattggtt gagagggttg agaagattta aaaggtatag
     7921 attcctcaaa acccgtcagg gcgataaaat gatagcgatg ggtgcgtaag ctcttttaaa
     7981 aataaagatt gcatttatga tttttccggt attattttcc ttcctcttat acatacatta
     8041 ccccgttatg ataaagtaac attcaagtaa caactccgta acggaactac ggaattgtta
     8101 cggaagtgtt acggtgtcgt tacttaattc actaaataac aattaattat gcatatttct
     8161 gcgcttatgg acaaaatata gggctaaagc attaaattag atgggggtta tataataaat
     8221 aaagggacta aatacttttt cgttatcctt tttttgccga cactaatatc tctgaaatat
     8281 aattgccctt ccggaattga aattttatga atatttcccg aaattgaagc tctcaaaaaa
     8341 tgaacgaaac tctgttcctg aacctatctt cggaatattt ttagggattt aagataagtt
     8401 tctcttatca attcaaatac agaaatctat tgacagaata gtgctattta agaattataa
     8461 caaaaatggc tatttgtgta tgatttatta tctttacagg aaatgatatt gagcagggat
     8521 tatgcgtaat ttattattgc aagagccggt tctggtagtg gacataggaa atacaaatat
     8581 cgtatgtgcc atatacttga aaggtaaatc catttggacg gtaagtctga aatccagtcg
     8641 ggaaaatact tctgacgaat attatgttct gctcggcagt ttgctggagt ccacaactct
     8701 accggatggt tataaactcg ggctaaaaga cattcgctat attgttttag gcagtgtagt
     8761 tcccgagctt acaagggttt ggaagcattt atgcagcaaa tatatacagg cagaagtgta
     8821 tgaaataaat gcacttagtc ctttaggaat cagtttcaga gtggataatc cctcttttat
     8881 cggggcagat ttaattgcca atgcttatgc tgcctggcag aaatatcgga catcttcaat
     8941 cataatagat ttgggaacag ccacaacgat tgaacttatc agcccagaag gaatgtttga
     9001 aggtgctata attgctcccg gaataaaaat tggagctgaa agtttattca gtaaagcagc
     9061 aaaactatat gaaattgagc ttgtttctcc tacggctctt ctgggtttga atactacgga
     9121 agcaatgctt tcaggaataa tttacggtca ttcttttatg ctggatacat tcattgctaa
     9181 aatcaaagag caatattcac aactaaaacc atttcaaaca atccttaccg gtggtatgtc
     9241 ttctttgata aaaccctacc ttagtgaaat taatactatt gataaaggtc ttactctgga
     9301 cgggttttat ctggctttag gcaaaatatt gaatcaataa tcctgaattt atgacgaaaa
     9361 agcttttcgc ctgtttgatt ttattagctt taactatatt gtgggctaca gagacggttc
     9421 ctgagggtgc taatccaccg gaagacgatc aaacagagca aaaaggcatt tttcctgcca
     9481 gatatttcag acccaaaaag gaaataaaac acgagcttga tcccttaaca gaaaaagcat
     9541 tatacccccg tctttacctt actgaataca atcctattat taacaatcgg atagaagttt
     9601 ataagataaa caaaggtatg aattattaca agacaaagcc ctatgaaatt cctgcctttg
     9661 caccagaata taaggaagaa attgattttg ccaatagtaa agtaatttta agcgttcaag
     9721 taggtaatta taaaattgca cccgatgttc cgattagttt tgaccgttat ttcagcaata
     9781 tgcaaacaaa ggtttttcat aaatccctga ttgctaattt caagacccaa gaacaagtag
     9841 tgcagacagt taccagcggt ttatttaaag accttacttt attgccggaa attgctatgc
     9901 cgaaagcgat gcaaaaggtt cttggttctt ctgcaggacg cttaaatctg gatggaactc
     9961 aaaaacttac tatgcaggtt agtaataccc atagaaaaca ggttcccata tatgatcaat
    10021 cagggaaaaa tgtgtttgac ctgaaaatgg aaatggaaac caaccttcgt ctttccggaa
    10081 ctatcggtga taaaattgct gtaaacttta aatacaattc caagcaggat gaagaactct
    10141 ttgatatgaa caatgtaaat gtaaaatata cgggctatga tgacgaattt atccaaagta
    10201 ttgaaggggg aaatattgct ctttctttaa gcggatcgcg ctatgtaagc tattctgcca
    10261 gttcacaggg actttttggt ataaccagta aattcaaata tggcaatttg gacttaagtg
    10321 taattgccag taaagaagaa agccaaaaaa atactcagac ctacatagga aaaagtcagg
    10381 cggattcttc cacggtcagc agctggaaat atgccagaag aacaatgtat tatttgcacg
    10441 atccctatca actttaccaa cttaaaaccg gaactaatat acctccaggt tggataaata
    10501 atgcaattga aaccgctcca gacggttcct ggatggtaaa tgtaaatttt ttgccggcaa
    10561 atggaactgt gcgtttgttt tatgatgatg ccaattatac caataatacc gcttccgcag
    10621 taggagatac tatctactat tccaataata tgagacc
//
LOCUS       DAOPFL010000058        10558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_7681_length_10558_cov_85.351899, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000058 DAOPFL010000000
VERSION     DAOPFL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7681_length_10558_cov_85.351899"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLG69_05200"
     CDS             <1..326
                     /locus_tag="PLG69_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPI42941.1"
                     /translation="QVLDEGWMISFTANITYANSKLDDVIRLVPLDRFMVETDCPYLP
                     PYLHRGKRNSPLFLPLVVEKIAEVKEISPNEVAELSFANAYRFFRVPPDPVKTSHKKA
                     GHKKA"
     gene            391..1596
                     /locus_tag="PLG69_05205"
     CDS             391..1596
                     /locus_tag="PLG69_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42942.1"
                     /translation="MKKIVIVICGIVLGVSLWAGNSIFSFYGFPVRNYGRDIYSFGMG
                     DTGASDVFRLNTGYANPAMHNRNNYTLFSTGMILGYTKYQSRFEGEKHSYRDDSLDFP
                     YFNISIPINKHRLGVQFNSLSSGLVTNQRTLPDGSTETQTTDKYLYKADLIYSYSFKD
                     VNLGMSVNYYFGHNKLYFSQTGNYSNFDTYESLLMDFKNPSVTFGAIKIFDKYSIGAH
                     YNAPVTMKGELIRSCIHNTEPAVDYEYKLPELYCVSTTVKPLEQFKITADISYEPWES
                     ISHNYRDCYKAGIGFAYEPDTETHKHIFLKLPMRLGGSMRQLPFYDKNGNDIDEYAIS
                     CGLTLPLKNEVDRIDLGFQYLQRGALSTNNLSDTSMMLMIGFTGFDIISKGIDRTAPR
                     DIPEKEEVE"
     gene            1593..3155
                     /locus_tag="PLG69_05210"
     CDS             1593..3155
                     /locus_tag="PLG69_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPI42943.1"
                     /translation="MKQDEPNSNINISNDLLNQIIKSRNLTSLNLQTNLEMLPDEKLF
                     ANIDKVENRMRIALFENEPMVIFGHDDPDGITSTYILYNFLNSCGYQKHCYYIPNRNL
                     EPHGIQDGFIEFVRQNDYRLIVTVDNGIVSKEGVKNLNAMGCEVIITDHHIVQPDMLP
                     PAFTIMNPQLPECPYPFKQLAGVGVVLMLIRYLGRLWNHPVEPASYFWTAVGSIADKV
                     PMIGLNRILVRYVLEHFAEVQDETVTFLLRNYNRINTMSDIFSFLQYTSHLIANGREP
                     NGQHTAMRFILQLSDAKAILFEDLESQKNKWESELNRIFKFIDTLATDFVGNWFVYYD
                     DEDVIPYTLLGTAATYVVSKLHIPAIILKYHNGVTVCEGRCGEDFNIMDAFTHCKKHL
                     AQFGGHPRAAGFTMKPEHYDAFLECFNSFLQKNYHPSKQEILSYDAEVCPKDLNWDNW
                     KKLEILLPWGQLNPEPSFLIRNTSRAEITRYVSLDNSGMDLPNKGKGDALVLWKAPNL
                     VKVLSWQQKINE"
     gene            3177..3764
                     /locus_tag="PLG69_05215"
     CDS             3177..3764
                     /locus_tag="PLG69_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPI42944.1"
                     /translation="MIEPGETLRKAIHISSLVIPLGYRYLLSFNRAWGFSLLLIAFTI
                     SLVIEFYRFWQKDFHKTFYRIFGILLRKHERKDFTGATYMLFSAVICVAFFNPLIASC
                     AMAFLTIGDTFAAFVGINFGKRRFNNGKKSLEGSLACFVSCLIFGIFWLDNPPLAIFG
                     ALTATIAELCNIPLDDNIKIPIASALVMTLLSIVI"
     gene            3778..4767
                     /locus_tag="PLG69_05220"
     CDS             3778..4767
                     /locus_tag="PLG69_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPI42945.1"
                     /translation="MKLSFIIPVLNEQESLKELYTEIVQNCQGYEYEIIFIDDGSVDN
                     SFAVMQELAENDEKVKAVKFRRNFGKATALQVGFNLAEGDIIFTLDGDLQDNPMEIPN
                     FIAKLNEGYDLVSGWKRKRRDPLYKILPSKLFNFVTAKTFHLKLKDYNCGFKAYRHPL
                     EKELVLYGEMHRYIPVLAHSLGYKVGEIPVAHRPRKFGKSKYGKERYLRGFFDLLTVK
                     MITQYSKSPLYLFGRVGIASTFIGLIISIYLAILKLFYGQPLSNRPLLFLGILLILGG
                     LQFISMGLISELIVNRFRQGNRTPVSITHCLNIESPVPLLESKQPFIELGDRK"
     gene            4809..6032
                     /locus_tag="PLG69_05225"
     CDS             4809..6032
                     /locus_tag="PLG69_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI42946.1"
                     /translation="MDKLSTFFLTRYLKAPKRNLLRFSFVFMILGIILSVGILSAGLN
                     LFEGYERTLKDVLLGTFPHITLTKANLDNISNDETDSLITKLSKRYEIQQITPLLSYP
                     VMTAGKEKVRGAILNAYDFNGKQPFPQAKYIQKGKKIPAKGEVIIGKYLAKELGKDIG
                     DTLKVVFTRLDNISALGIFPSEYYLPIAGIYSSGFYETDRSLVLTNITDAEYMLNAKP
                     GFSKLEIRLKSKDIEKAPEIAQLLQTIAGPEYSVYPWQTFSAGLLHLVEMEKWLIFII
                     FCFLVLIAGINVISAVATIILDKKNEIAVLKTLGANSASIKRILCLQVGLSALLAIIA
                     GLGFGALLSWGIEKQNFYQLKGDVYFIDSLNTSIIPLNQIIIFVVSSVLIFICIYFPL
                     KQIDKMEIIELLRNP"
     gene            6082..6753
                     /locus_tag="PLG69_05230"
     CDS             6082..6753
                     /locus_tag="PLG69_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI42947.1"
                     /translation="MICLAGYDLCKSYTDSNQTINVLRKATLEVQESELVCITGKSGS
                     GKSTLLHLLGLLDSPDSGKVMIGGNYVQSTMPEAASIRNLELGFVFQFHYLIEDLTAQ
                     ENVALPMIISGVSHSKAIANAVSLLNELDLAERITHYPNQLSGGEQQRVALARALINN
                     PKIVLADEPTGNLDPEHSNEIWDMILKLNQEHGQTFVIVTHDVANAFKAQVTYNLSEG
                     KLEKR"
     gene            6769..>10558
                     /locus_tag="PLG69_05235"
     CDS             6769..>10558
                     /locus_tag="PLG69_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42948.1"
                     /translation="MPKAKKFLIFVLILLLINTGFFLAWYAFGLRNYCKNYLARYLGK
                     LTKGEVTIDELHISDRQLLAQGITYTSADSSIAVKVHRLSVQYNLTRYLFSGFKTSKL
                     ISDIEIYKPTVHYSYFYKPKPPKAKKPLVLPDFAHYFKRLQLTEGTAVISAEIPVKII
                     QEGYLSISDSFQGINLTAINKSTSDVTLNALSAKGGKLTAKGLLDKGRLAKVDAEIAS
                     FRPLSVSHPDLQNLQTEISLVASLTQDTLKAPLKYKAEAQIWGTETLFAGNYPVLIPY
                     LWVKTDGRNLNLSLSHSNFGSSEMEGEVRISNLDQKMKFDYATATANIDLSMFHKELQ
                     GYVVVQAKGYGTIDKPELELNVTSTQAKYQKYAFRNLNLTGSYNNNELAFNLPELVFE
                     NQIINCSGNLNPLYRTINAHLETKPIKIKSQPYLATANLDVYAELWEKYPFVDATINQ
                     LDFNYDLATLQGVRGKVKLIPLASDNNLYLDANISGDNGFNIKVIGDIISRNLLLDAK
                     FDNLEVAELYSQPKVIELNPYLNGDIKAIITGENVLLQTLLGVKLDKPVPVNTPLDAL
                     GTFNYKTKEASLHLIGKNGMMNYQPLNFELSAMLKDNLLTVQGFKFNDFISLSGAINL
                     SNYRDLNFALNLKNIGYLDIVHYFPQLDINLPEFYGLTVFAEYNRENLGELKANAYLS
                     YIDLLAVTPFSLKLELNGPVDSIQVAGNIHNSEQNILNLSGLIGFQPELNISLDAGFQ
                     DLAIQNVIINSPLTGKLNGIAGITWHKIEERKPELELRANLLSEDITIKNYTINTAII
                     QGKQTPQQLIVDSLFVQSAGLFKLNGSGAINYNALYNEFFEGPEQLNLNVEGELFGWL
                     KNLTPYILEAKGRSSLSISIGTSEDQFLVSSGKININNGFIRLKDQTEPLANINIRGG
                     FDKNRLIIEYGQVQMGEGKLIFNNIFEADNSDHFMLGFLDLGIFRAMCEEPGILVNIP
                     KYTTPRSLTKVILRGQNSRYATIRGPFDQMKISGEAVASNTNVLYPPDTENLLTLAST
                     VRGSTKKKTSEPNPLPFILDVKLIVGENVKYVTYPAELRVVPGGYLHLAYDGLSFIVK
                     EASFSSEQGTVDIFGTIFQVEKVNLTLVDSQNLLGLEGIFYKRAPDGSMITLSAITSS
                     DYTKSFMERLQLSLTSDNPEDKTIGQILARLHYTGTETPDRTQGEFWQDEALGLISGN
                     LDASLFTPILSPIENYIRRTLHLDSFTINAGFIQNLYTEYTNNPQQFAEYTDMNQLSS
                     D"
BASE COUNT         3205 a         1960 c         2015 g         3378 t
ORIGIN      
        1 aacaggtttt ggatgagggt tggatgattt cttttacggc taatatcact tatgctaatt
       61 ccaaattgga tgatgttatt cgtttagttc ccttggatag atttatggtg gaaacggatt
      121 gtccctattt gcctccctat ttacaccgcg gaaaaagaaa ttcacctctt ttcctgcctt
      181 tagttgtaga aaagatagca gaagttaaag aaatcagtcc caatgaagta gcagaactct
      241 cttttgcgaa tgcttatcgt tttttccgtg ttcctcccga ccctgtaaaa acatcacata
      301 aaaaagccgg acataaaaaa gcttaatgtt atatataggt taactaatta gccggcaaga
      361 tattatgact ttaaagtgaa aggatatata atgaaaaaaa tagttatcgt tatctgtggc
      421 atagtattag gtgtatcact gtgggcagga aattccatct tctcctttta tggttttcct
      481 gttcgtaatt atggcagaga tatttacagc tttggtatgg gtgataccgg tgccagtgat
      541 gtgtttcgtc tcaataccgg ttatgcaaat cctgcaatgc ataatagaaa taactacact
      601 ttattttcta ccggaatgat tttgggctat actaaatatc aatcccgttt tgaaggcgag
      661 aaacatagtt atcgggatga ttccctggat tttccctatt ttaatatcag tatacctatt
      721 aacaaacacc gtttgggagt tcaattcaat tctctctctt caggattggt tacgaatcaa
      781 agaacccttc ccgatggttc aacggaaacc caaaccaccg acaaatatct ctataaagcc
      841 gatctgattt acagttacag ttttaaagat gtaaacttgg gaatgagtgt aaactattat
      901 tttggacata acaaacttta tttttcgcaa acgggaaatt attccaattt tgatacttat
      961 gaatccctgc tgatggattt caagaatccc agcgtaactt ttggagcaat taaaattttt
     1021 gataaatata gtatcggtgc acattacaat gctcccgtaa caatgaaagg tgaactcatt
     1081 cgcagttgca ttcataatac cgaaccggct gttgattatg aatataaact gccggaactt
     1141 tattgtgtaa gcacaaccgt taagcccttg gaacaattta agataactgc agatatcagc
     1201 tacgaaccct gggaatcaat ttcccataac tatagggatt gttataaagc aggaatcgga
     1261 tttgcttatg agccagatac tgaaactcat aaacatattt ttctgaaact gcctatgagg
     1321 ttaggtggct ctatgcgtca gctccctttt tatgataaaa atggcaatga tattgatgaa
     1381 tatgctatca gctgtggact tacccttccc ttgaaaaatg aagtagatag aattgattta
     1441 gggtttcaat atttacaacg aggtgctctt tccaccaaca atcttagcga tacttcaatg
     1501 atgctcatga tcggatttac cggttttgat attatcagca agggaataga tagaactgct
     1561 ccgcgtgata tacctgaaaa agaggaagtt gaatgaagca agatgaacct aatagtaata
     1621 taaatatatc taatgattta ttaaatcaga ttattaaatc ccgtaacctt acttccctga
     1681 atttgcagac caatcttgaa atgctacccg atgaaaagct ttttgccaat attgacaagg
     1741 tggaaaatag gatgcggatt gctctttttg agaatgaacc gatggtgatt ttcggtcacg
     1801 atgatcctga tggaataacc agcacctata ttttgtataa cttcttaaat tcctgcggtt
     1861 atcaaaaaca ttgttattat attcccaatc gcaatctgga gccccatggc attcaggatg
     1921 gttttattga atttgtgcgt caaaatgatt accggttaat agttacagtg gataacggca
     1981 ttgtttccaa agaaggtgta aaaaatctga atgcaatggg ttgtgaagta ataataaccg
     2041 atcatcatat tgtgcagccg gatatgttgc ctcctgcatt tacgataatg aatcctcagc
     2101 tgccggaatg tccatatcct tttaaacagc ttgccggagt aggagttgtt ttaatgctga
     2161 ttagatattt gggacgcctt tggaatcacc ctgttgaacc tgccagttac ttttggactg
     2221 ctgttggctc tattgctgat aaagttccaa tgattggttt gaacagaatc cttgttcgct
     2281 atgttttaga acattttgct gaggttcagg atgaaacagt tacttttctt ctgcgtaatt
     2341 ataaccgtat caatacaatg agtgatattt tcagcttttt gcaatatact tcccatctta
     2401 tagctaatgg tcgtgaacct aatggccagc atactgcaat gcgctttatt ctgcagttat
     2461 cagatgccaa agctatactt tttgaggact tggaaagcca aaagaataaa tgggaaagcg
     2521 aattgaacag gattttcaaa tttatagata ctctggcaac ggattttgta ggtaactggt
     2581 ttgtttatta tgatgatgaa gatgttattc cttacactct tttgggaact gcggcaactt
     2641 atgttgtaag caaactacat attcctgcta taatcctaaa atatcataat ggcgttacgg
     2701 tttgcgaagg cagatgtgga gaggatttta acataatgga tgctttcact cactgtaaaa
     2761 agcacttagc acaatttgga ggtcatccca gagcagcagg atttacaatg aaacctgaac
     2821 actacgatgc ttttctggag tgtttcaaca gctttttgca gaagaattat cacccttcta
     2881 aacaggaaat attaagctac gatgcagagg tatgtcctaa agacctgaac tgggataatt
     2941 ggaaaaagct ggaaattttg ttaccttggg gacagttgaa tccggaacct tcatttttaa
     3001 ttcgtaacac ttccagagct gaaattaccc gttatgtatc tttggataac agcggtatgg
     3061 atttgccgaa taagggaaaa ggcgatgcgt tagttttgtg gaaagctccc aatctggtta
     3121 aagtgttatc ctggcagcaa aaaatcaatg aataaattaa caggaggcat ctgcagttga
     3181 ttgagcccgg tgaaacccta agaaaagcaa tacatatcag cagtttagtt atccctttgg
     3241 gttatcgtta tctgctgtct tttaatcgtg cttggggttt ttcactcttg ctgattgcct
     3301 tcactattag tttggtaata gaattttacc gtttttggca gaaggatttt cataagacct
     3361 tttaccgcat ctttggtatc ctgcttagaa agcatgagcg caaagatttt acgggtgcta
     3421 cttatatgct tttttcagca gtgatttgcg ttgctttttt caatccgctt attgcttcct
     3481 gcgctatggc ttttttaacg attggcgata cttttgctgc ttttgtagga attaatttcg
     3541 gtaaacgccg gtttaacaac ggaaaaaaaa gtttggaagg cagtttggct tgttttgtca
     3601 gttgcttaat tttcggcatc ttttggctgg ataatcctcc actggctatt ttcggtgctt
     3661 taaccgctac aatagcagaa ttatgtaaca ttccccttga cgacaatatc aagattccaa
     3721 ttgcttcagc tttagtaatg actttgctga gcattgtaat ttaaagataa ggtagctatg
     3781 aaattatcct ttataattcc ggtcttgaat gaacaggagt ccttaaaaga actctatact
     3841 gaaattgtgc aaaactgcca gggctatgaa tatgaaatca tctttattga tgatggctct
     3901 gttgataaca gctttgccgt tatgcaggaa ttggcagaaa acgatgaaaa agttaaagca
     3961 gttaagttca gacgcaattt tggcaaagct acagctcttc aggttggttt taaccttgcc
     4021 gaaggagata ttatttttac cctggatggc gatttacaag ataatcctat ggaaattccg
     4081 aattttattg ccaagcttaa tgagggctac gatttggttt ccggctggaa acgcaaaaga
     4141 agagatccgt tatataaaat cctgccttcc aaattgttca atttcgttac cgctaagacc
     4201 ttccacttga aattaaagga ctataattgc ggatttaagg catatcgtca tcctctggaa
     4261 aaagaacttg tcctctatgg agaaatgcat cgttacatac ctgttttggc tcattccctg
     4321 ggctataaag ttggcgaaat tccggttgct catcgtccgc gtaaattcgg taaaagcaaa
     4381 tatggtaaag agcgttattt gcgcggtttt tttgaccttc taaccgtaaa aatgattacc
     4441 caatacagta aaagtccttt gtatctcttt gggcgcgttg gaattgcctc cacttttatt
     4501 ggtcttataa ttagtattta ccttgctatt ttgaaattgt tctatggtca gccgctttcc
     4561 aatcgtcctc ttttgttctt aggcatttta cttattttag gtggtttaca gtttatttct
     4621 atgggcttga tttcagagtt aattgtaaat cgtttccgcc aaggaaacag gacacctgtt
     4681 tctatcactc attgcctgaa tattgagtcc ccggttccct tgttggaatc caagcaaccc
     4741 tttatagaat taggagacag gaaatgatga gaacatcatt ttccatctcg ccatctcact
     4801 aattagttat ggataagctt tccacttttt tcctaacacg ctatctcaaa gctcccaagc
     4861 gtaatttatt acggttcagt tttgtgttta tgattttggg aattatcctt tctgtaggaa
     4921 ttttaagtgc cggtttgaac ctctttgaag gttatgaaag gactttgaag gatgtcctct
     4981 tgggaacttt cccccacatc actttaacta aagcaaatct ggataatata tccaacgatg
     5041 aaaccgattc gctgataact aaactttcta aaaggtatga aattcagcaa attactcctc
     5101 tgcttagcta ccctgttatg acggctggaa aagaaaaagt gcggggtgca attctcaatg
     5161 cttatgattt taacggtaaa caaccctttc cgcaggcaaa atacattcaa aaaggtaaaa
     5221 aaatacctgc taagggagaa gttattattg gtaaatatct ggctaaagaa ctgggtaaag
     5281 atattggaga tactttaaaa gttgtcttta cccgtttaga taatatttcc gctttaggca
     5341 tttttccttc agaatattat ttaccgatag caggcattta cagcagtgga ttttatgaaa
     5401 cagaccgctc tttagttctt accaatatta cggacgccga atatatgcta aatgcaaaac
     5461 ccggtttcag taaactggaa atacgcttaa aatcaaagga catagaaaaa gctcccgaaa
     5521 ttgctcaact cctccaaaca attgcaggtc ccgaatattc agtataccct tggcagacct
     5581 tcagtgccgg tttattacat ctggtagaaa tggaaaaatg gctgattttt attattttct
     5641 gctttttggt tttaattgcc ggaataaatg ttatttctgc tgtcgcaacc atcattctgg
     5701 ataaaaagaa cgaaattgcc gtccttaaaa ccctgggagc caattctgcc tccattaaaa
     5761 gaatactttg tctacaggta gggctctctg ctttattggc tattatagca ggtctggggt
     5821 ttggcgcact tctttcctgg ggtatagaaa agcagaattt ttatcaactg aaaggtgatg
     5881 tttattttat tgacagctta aacacgagta tcattccctt aaatcaaata ataatttttg
     5941 ttgtttcctc ggttctgata tttatttgta tttatttccc tcttaagcaa atagataaga
     6001 tggaaattat tgaattactg cgcaacccat aacggattat attatgaaaa agcgattgtt
     6061 ctattttata aggattttag tatgatttgt ttagccggtt acgacctctg caaaagttac
     6121 acagacagca atcagaccat caatgtttta cggaaagcta ctcttgaagt gcaggaatcc
     6181 gaattggtct gtattacggg aaaatccggc tcgggaaaaa gtactctcct ccatcttttg
     6241 ggtttactgg attccccgga ttccggaaaa gtaatgattg gtggaaatta tgtgcagtcc
     6301 actatgccgg aagctgcttc catacgcaat ttagagctgg gatttgtttt ccagtttcac
     6361 tacctaatag aagacctcac agcccaggaa aatgtagctt taccgatgat aatatctggt
     6421 gtaagtcatt ccaaagcaat tgctaatgct gtttcacttc taaatgaact tgatttggct
     6481 gagcgcatca ctcattatcc caatcaatta agtggtggcg aacagcaacg ggttgctctt
     6541 gccagagctc tgatcaataa ccctaaaatc gttttggcag atgaaccgac agggaatctt
     6601 gacccggaac acagcaatga gatttgggat atgattttaa agctcaatca agaacacggt
     6661 caaaccttcg ttatcgtaac tcatgatgtt gctaatgcct ttaaggcaca ggtaacttat
     6721 aatttaagcg aaggcaaact ggaaaaaaga taaactggtc attctgctat gccaaaagca
     6781 aaaaagttcc tgatttttgt gctgattctc ctgttgataa acacgggctt ctttcttgct
     6841 tggtatgctt ttggtttaag aaattactgc aaaaattatc tcgccagata tttggggaaa
     6901 ttgaccaagg gtgaggtcac tattgatgaa ttgcatatta gtgaccgtca gttattagca
     6961 caaggtatta cttatacttc ggctgatagt agtattgccg taaaggttca tcgcttaagt
     7021 gttcagtata atcttacgcg ttaccttttt tccggtttta aaaccagtaa gttaattagt
     7081 gacatagaaa tatacaagcc cacggtacat tacagttatt tttataaacc aaaacctcct
     7141 aaagccaaaa aacctcttgt tcttcccgat tttgctcatt attttaagcg tttgcagtta
     7201 actgaaggaa cggctgttat ttctgctgaa ataccggtta aaattataca ggaagggtat
     7261 ttaagcatat cagacagttt tcagggaatt aacctgaccg ctatcaataa atccacttcc
     7321 gatgttacct taaatgcctt aagtgccaaa ggcggaaaat tgaccgctaa gggcttatta
     7381 gataaaggac gcttggctaa agtggatgct gaaattgctt ctttccgacc tctttccgtt
     7441 tctcatcccg atttacaaaa tctgcaaacg gaaatttcct tggttgcatc cttaacgcag
     7501 gataccctaa aagctcctct taaatataaa gctgaagctc aaatttgggg aacggaaaca
     7561 ttatttgccg gaaattatcc tgttctaatt ccctatcttt gggttaaaac cgatggtaga
     7621 aacctcaatt tatccctgtc tcacagcaat tttggcagta gtgaaatgga aggagaggtt
     7681 agaataagca atttagacca aaaaatgaag tttgattatg ccactgccac tgccaatatt
     7741 gatttgagta tgttccataa ggaacttcag ggctatgtag tggtgcaagc taaaggatat
     7801 ggcactatag ataaacccga attggaacta aatgttacct ctacccaggc aaaatatcaa
     7861 aaatatgctt ttcgcaatct taatttaacc ggttcctaca acaataatga gcttgctttt
     7921 aatttaccgg aactcgtttt tgagaatcaa attattaact gttcaggcaa tctaaaccct
     7981 ctttacagaa caatcaatgc tcatctggaa acaaaaccga taaaaataaa gtcccagccc
     8041 tatctagcaa ctgctaattt ggatgtttat gcagaattat gggaaaaata tccttttgtt
     8101 gatgccacta tcaatcagct ggattttaat tatgacctgg caacgcttca aggagtaaga
     8161 ggtaaagtaa aattaatacc tcttgccagt gacaataatc tatatttaga tgccaatata
     8221 tcaggagata acggttttaa tattaaagtt ataggtgata ttattagtcg taaccttcta
     8281 ttagatgcaa agtttgataa cttggaagtg gcagaactct attctcaacc caaagttatt
     8341 gaactgaatc cctatctgaa tggagacata aaggcaatca ttacaggtga aaatgtcctt
     8401 ttacaaacac ttttgggagt aaaattagat aaacctgtgc cagtaaatac ccctttagat
     8461 gccttgggaa cctttaatta taaaaccaaa gaggcctctt tacatcttat cggcaagaat
     8521 ggaatgatga attatcagcc cctaaatttt gaactttctg caatgctgaa agataacctg
     8581 cttaccgttc agggttttaa gtttaatgac ttcatcagtt taagcggagc aattaatctc
     8641 tccaactatc gggacctgaa ttttgcctta aatcttaaaa acatcggtta tttggatatt
     8701 gtgcattatt ttcctcagct ggatatcaat ctgcccgaat tttacggact aactgttttt
     8761 gccgaataca atcgtgaaaa tttgggagaa ctgaaagcaa atgcctattt atcttatatt
     8821 gaccttttgg cagttactcc tttttctctg aagttagaat taaacggtcc tgtagatagt
     8881 attcaggttg cgggtaatat tcacaattcg gaacaaaaca ttctgaattt aagcggtctt
     8941 attggtttcc aaccggagct gaatattagt ttggatgctg gtttccaaga cctcgctatt
     9001 caaaatgtta ttattaattc tcctttaaca ggaaaattaa atggaatagc aggaattacc
     9061 tggcataaaa ttgaagaacg aaaaccggaa ttggaactga gggcaaacct tctttcggaa
     9121 gacatcacta taaaaaatta taccatcaat acagctatta ttcaaggtaa acaaacaccc
     9181 cagcaactca ttgtagacag tttatttgtg caatcggcag gactttttaa gcttaacggc
     9241 tcgggtgcaa ttaattataa tgcactttac aacgaatttt ttgaaggacc cgaacagctg
     9301 aatctgaatg tggaaggtga actttttggc tggctgaaaa atctcactcc ctacatttta
     9361 gaagcaaaag gaagatcctc tttaagcata agcattggca ctagcgaaga tcaatttctg
     9421 gtttccagcg gaaaaattaa cattaacaat ggatttattc gccttaaaga ccaaacggaa
     9481 ccccttgcca acatcaatat tagaggcggt tttgataaga atcgcctgat tattgaatat
     9541 ggacaggttc aaatgggaga aggcaaactc atttttaata atatctttga agctgataac
     9601 agcgaccatt ttatgctggg attcctggat ttgggaattt ttagggcgat gtgtgaagaa
     9661 cctggtattc ttgtaaacat ccctaaatat acaactcccc gttctttaac caaagtaatt
     9721 ttacgaggcc aaaatagtcg ttatgccaca atccgaggtc cctttgacca aatgaaaata
     9781 agcggagaag cagttgcttc caacaccaat gttctttatc ctccggatac cgagaattta
     9841 cttactttgg catctaccgt tcgtggttcc accaagaaaa aaacctccga acccaacccc
     9901 ctgcctttta tattggatgt taaactcatt gttggagaga atgttaagta tgtaacctac
     9961 cctgcagaac tgagagttgt tccgggtgga tatttgcatt tagcttatga tggcttatca
    10021 tttattgtta aagaagcgtc tttctcttcg gaacagggaa ctgttgatat tttcggcacc
    10081 atctttcaag tggaaaaagt gaatttaacc ttagttgact cccaaaatct gttaggtttg
    10141 gaaggtattt tttataaacg agcacctgat ggttctatga taactctttc ggcaataaca
    10201 tcttccgatt atactaagag ttttatggaa cgcttgcaat taagcttaac aagtgataac
    10261 cctgaagata aaaccatcgg tcaaattctg gctcgtttgc attatacggg aacagaaact
    10321 cccgatagaa cacagggtga attctggcaa gatgaagcat taggtttaat ttccggtaac
    10381 cttgatgctt cacttttcac tcccattctt tcacccatag aaaattatat tcgcagaact
    10441 ctgcatttgg atagcttcac cataaatgcc ggttttattc aaaatcttta tactgaatat
    10501 accaataatc ctcaacaatt tgctgaatat acagatatga atcagcttag ttccgata
//
LOCUS       DAOPFL010000059        10407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_7845_length_10407_cov_103.547044, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000059 DAOPFL010000000
VERSION     DAOPFL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7845_length_10407_cov_103.547044"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..996)
                     /gene="ftsY"
                     /locus_tag="PLG69_05240"
     CDS             complement(31..996)
                     /gene="ftsY"
                     /locus_tag="PLG69_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPI42949.1"
                     /translation="MSTIIIKNGDKKVLNIVKNLKEKLAKTNSNFMGKITETVKKRKV
                     VDEEMLAEIEEILLSCDTGIEMTDIILTRFKEQLKKDKVTDPEIAQIYLTDVMRDILL
                     AENEETPDFFAEPQAKPYVVVFVGVNGTGKTTTIGKVAYKFNQMGKKVLIVAGDTFRA
                     AAIEQIAIWAERAGVPIVRSQPDSDPAAIIYDGVHSALARGYDVVLIDTAGRQHTKDN
                     LMKELSKIERTIQKLIPDAPHEVILVVDATTGQNAVSQAHNFDKAMKLTGIALTKYDG
                     TSKGGIIFNLKYNLQLPVKLLGVGEGIEDLLPFKAVPFVKAFFEE"
     gene            1101..>1399
                     /locus_tag="PLG69_05245"
     CDS             1101..>1399
                     /locus_tag="PLG69_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42950.1"
                     /translation="MERTKILPRDLLLLFFPIIAAILGGLLFSGSFTEFTDNWGGKGI
                     SQAELYFALSFFIGALAFGYSCLPKNVLLGLQIFIPLLYGLLMLLRFKVELSLFLL"
     assembly_gap    1400..1499
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1500..2143
                     /gene="trmB"
                     /locus_tag="PLG69_05250"
     CDS             <1500..2143
                     /gene="trmB"
                     /locus_tag="PLG69_05250"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPI42951.1"
                     /translation="RTKKTMISDRDFFVIKPQINEQGKFISLNPVDLFHNSHSLYLEI
                     GCGKGEFIARYSILHPEYNYIGLEAAEKRINNTLKKLTPEQNPNVRLMRLYVDNSICQ
                     LFPAESVSGVFIQYPDPWPKRKHHRRRLIQQEFLTSLAMILKPLAQVQITTDNSDYAA
                     WILDEFLAHPSFISVYEELMLKESPFEEHIPTWYESEQRRQGFEPQYMLFKRI"
     gene            2156..3847
                     /locus_tag="PLG69_05255"
     CDS             2156..3847
                     /locus_tag="PLG69_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPI42952.1"
                     /translation="MSYNFSSNYQINKMLNSVVESITNLAEEQLLHIQRLTRIGESLS
                     SETDLDKIFDMILEEGISFTRADAATIYTVNEDATKLQFEIVYNATLNLRKGGTRGPV
                     NWKSIDLYDEKGNPVDNKIVTNVYHHKQSLCFADVYKAEGYDVSGTIKTDRENNYRCK
                     SMLTIPLKNHEDTVLGVIQFINAMDEEGNIVSFTDEHKAMLSSLASQAAIALSNKKLI
                     ENLEELLNQFIRSIAGAIERKSKYSSNHITRVAMLTEMIATKINEKGEKYFPGRKFNE
                     NELKELSMAGWMHDVGKIVTPEAIMDKSTKLETICDRIELVKLRLEKLELLLTYLQLK
                     LKEDEFAAFIHQNIDSELEPTGFRNYLANITNFLINVNVGKEFVDKADLEKVEKLGKI
                     NFEYEGKRYFIFTEDEKKNMQISGRGTFTPEEMKIMQDHVAITWEMLSQLSFPKKYEN
                     VAFYAATHHEALNGKGYPNGYTADKLPLQSRILAVADIFEALTASDRPYKTAKTLSES
                     LNIMGYMVKDGHLDKDLVNFFLDSGLYKEYADRFMNKEYIDEVDIDSIKAIYTNQ"
     gene            3844..4809
                     /gene="dusB"
                     /locus_tag="PLG69_05260"
     CDS             3844..4809
                     /gene="dusB"
                     /locus_tag="PLG69_05260"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPI42953.1"
                     /translation="MTVPFLNKPLLFLAPLAGYSDSAFRQICKNWGAEVLYSEMVSAD
                     GITRNSAKTLQYVQFDESEHPFGIQIFGSNPLIMAKAVEVLLPLKPDFMDINMGCPVK
                     KVVRKGAGSALMQTPSLAGKIVKEVKNALLGRIPLSVKFRSGWNFQNQNYLQFGLMLQ
                     ECGTDFLCLHPRTSSQMFSGSSNWEQIKILKQNVQIPVVGNGDIKTPEDAQRMITETN
                     CDGIMIGRGALGKPWLFAQSRQLISTGTYNPVTQKEIVKTIFAHLDKALLYKSEDVVV
                     REMRAHLCFYTKGILGGAELRNQINHTENISELKAIILKGFSHIG"
     gene            5160..5765
                     /locus_tag="PLG69_05265"
     CDS             5160..5765
                     /locus_tag="PLG69_05265"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPI42954.1"
                     /translation="MKYFRKLVGEKCYLSPVSMDDVEKYTEWLNDMEIGQFVTLSHIV
                     LDIEKETELLRKLMREDYIFAIVEKDTNKVIGNCGIHQVSQVHRNASLGIFIGEKTFW
                     NQGIGTEATSLLLDFAFNILNLHNVYLSVMSYNKRAIRCYEKIGFKKVGVQREFMFVS
                     GQYHDVFIYDILASEFTSPYVKKIYDYSISDEAGRSKISIV"
     gene            5766..6878
                     /locus_tag="PLG69_05270"
     CDS             5766..6878
                     /locus_tag="PLG69_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPI42955.1"
                     /translation="MLPKIKGQENALELLDKAIENDRIAQAYLFYGSDGVGKFTTALY
                     FGMAVNCLATSELKPCGECDACHKFLQLEHPDFVYLFPTPNLNLSAEGEIKNTDAQKS
                     YEAYLQNKINTPWKDFFFKEATEIRKESITLLNKRLELSIHEAKYRIVIIEDADMMNN
                     ATANAFLKTLEEPPERTIIILITERLPLILPTILSRTQPVYFKPLTRGVIEEILINEF
                     EINSALARSASRICAGNLKTAIRIASESESLSSNWAFEIFSLAAEENDLGYLELAERY
                     KKQQTIDQVIDLLKYIRIIASDLGSLSANPESEITNIDKIDFLKPIAEANLGIDDRLY
                     DFLLFLEDLNRKVEGYANLNLVLTNLYLNCKHLLKP"
     gene            6879..8453
                     /gene="murJ"
                     /locus_tag="PLG69_05275"
     CDS             6879..8453
                     /gene="murJ"
                     /locus_tag="PLG69_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPI42956.1"
                     /translation="MEAKGTHRTLAKNISVMSIGVFISRIFGLIRDQVMAYFFGTTYL
                     NDAFNVGYNIPNLLRRLFGEGALSTAFVPLYNDIKIKQGKEKQIEFALNLLSVLTFIL
                     CILTILGIALAPLIVKCLYPGLASETKVLAIKLTRIIFPYLFFIGLSSTFIAILNSHN
                     YFFMTGLSSALLNIGMIATVLIPHFVLKVSGEDLIVWAGGGVLVGGFLQTVINLPYLK
                     RIGYRWAIYLKFGSEALSVLWKRFIPSMIGIGIREINLIADSLMASFLPTGSITALNF
                     GNRLMQLPLGIFAISTGTAVLPFYSRCVSTENYQELSESIRFSGLNIAYIMLPVTTII
                     LALGEDFVRILFESGAFQEDAVLMTSQALIFYSLGLIFYGLNQTITPVFYAYKDTKTP
                     VKIAAGMVALNITLNFILIQFMAHRGLALSTSITACVNFFILLFLIRKKMPEISFSGI
                     AINIIKSLLICCFLYLLLYISCNLFPVSGRISLLIRDAILSIITLGLFYLTGILIKID
                     YLNQAGKKLCQRFLKK"
     gene            8429..10234
                     /gene="uvrC"
                     /locus_tag="PLG69_05280"
     CDS             8429..10234
                     /gene="uvrC"
                     /locus_tag="PLG69_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPI42957.1"
                     /translation="MPAFSEEIETKLALLPEQPGVYLWKDKDGTIIYIGKALNLKHRI
                     KNYLNPTGKDLKTEHLIKNIADLDYIITNSEADAFILEATLVKRYRPKYNIMLKDDKR
                     YPFVKITVNEPFPRIFITRELLKDNAKYFGPYTEVRYLRNTLRDLEWIFPLRSCNRNI
                     PKIKYKQPCMNFQLGKCPAPCIGNISQKDYAKTVNQLLRFFNGRYTELLDELRTEMQN
                     ASAELRFEEAGKFRDRIIAIERIQKRQSVFNVEGGNIDIIGFYREEKRAVCVILKMKN
                     GAIIHQENYPLTNLDYEEPDSILASFLQLYYADREEMPEEVLLPFAPKDMDKLNSWLG
                     NKLQIPQRGEKTKLIAMAKTNAFNIVEESKLAHLRKANRTIFPVQELKEALKLPKLPR
                     KIVCMDISTIQGSDTVSSAVFFENGKSKKKYYRHFIIRSIETQNDYAAMQETMARFLT
                     ETQKEPQMKPDLIVIDGGKGQLSASEQILRASGNNDISIIALAKRAEEVFVPGNEQSI
                     ILPRSSSALRLLINIRDEAHRFAISLHRKRRSRRTLESELESISGIGEKNKFLLLKEL
                     GSVENIANANRETLSKIKGIGPKIAEKIYNHFHST"
BASE COUNT         3230 a         1841 c         2023 g         3213 t
ORIGIN      
        1 cattctcttt ttctctatgt gaaaaaagct ttattcttca aagaacgctt tcacaaacgg
       61 cacagcttta aacggcagca aatcctcaat cccttctcca acgcctaaaa gtttaaccgg
      121 taattgcaaa ttatatttca ggttaaaaat aatacctcct ttggaagttc cgtcatattt
      181 agtgagagca attccggtta atttcattgc tttatcaaaa ttatgagctt gggaaacggc
      241 attttgtccc gttgtggcat ctacaactaa aatcacttcg tgaggtgcat caggaataag
      301 tttctggata gtgcgttcaa ttttgctcag ttctttcatc aaattatctt tggtatgttg
      361 tctgccagca gtatcaatta aaacaacatc atagccacgt gccagagcgg aatgaacacc
      421 atcatagata atagcggcag gatcgctatc cggttgagaa cgaacaattg gcacacctgc
      481 tctttcagcc caaatggcta tttgttcaat agcggcagca cggaaagtat ctccagcaac
      541 gattaaaacc ttttttccca tttggttaaa tttataggca actttgccga tggtagtggt
      601 tttgccagta ccgtttacac ctacgaagac aaccacataa ggttttgctt gcggttcggc
      661 aaaaaaatcg ggtgtttctt cattttccgc cagcaggata tctctcatta catcggtaag
      721 ataaatctga gcaatttccg ggtcggtaac tttatccttt ttcagctgtt ctttaaagcg
      781 ggttaaaata atatccgtca tttctattcc ggtatcacag ctaagcagaa tttcttcaat
      841 ttccgcaagc atttcttcat caaccacttt gcgtttttta acggtctcag ttatttttcc
      901 cataaaattg ctattggttt tagcgagttt ctctttcagg tttttaacga tgtttagcac
      961 ttttttatct ccgtttttaa ttattattgt tgacattata agcaactgcg ttaaatttgt
     1021 atttaggggc tggaagtcaa tataaatttg tttcagtcct gttctgaatg cagttcagaa
     1081 aacaacaatg aaggttttgc atggaacgca caaagatatt acccagggat ttattactgc
     1141 tcttttttcc gataatagca gcaatcttag gggggttgct tttttcggga agttttactg
     1201 aatttactga taattggggt ggtaaaggca tcagtcaggc agaattatat tttgctctca
     1261 gtttttttat tggagcatta gcttttggat acagctgtct tcctaaaaat gtattattgg
     1321 gtttacaaat ttttataccg cttttatacg gtcttttaat gctacttcgc tttaaagtgg
     1381 agctcagttt atttttactn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1501 gaggacgaag aagacgatga tttctgatcg ggatttcttt gttatcaaac cgcagataaa
     1561 tgaacagggc aaatttatat ccctgaatcc ggttgatttg tttcataatt cccattcctt
     1621 gtatctggaa atcggttgtg gcaagggtga atttattgcc cgctattcca ttctgcatcc
     1681 ggaatacaat tatatcggtt tggaagcggc ggaaaaaaga atcaacaaca cactcaagaa
     1741 attaacgccg gaacaaaacc ccaatgtgcg tcttatgcgt ttgtatgttg ataattcaat
     1801 ctgccagcta tttcctgcag aatcggtttc cggagttttt attcaatatc ctgatccctg
     1861 gcccaaacga aaacatcatc gccgtcgttt aattcaacag gaatttttaa cttctctggc
     1921 aatgatttta aaaccgctag ctcaggttca aatcaccact gataatagcg attatgctgc
     1981 ctggatttta gatgagtttt tagctcatcc cagttttata tccgtctatg aagaacttat
     2041 gcttaaagaa tcaccttttg aagaacatat tcccacctgg tatgaatccg aacaaaggcg
     2101 acagggcttt gagccacaat atatgttatt taaacgaatt taggagtaaa cacagatgag
     2161 ttacaatttt agtagcaact atcaaatcaa caaaatgctt aatagtgttg tagaaagcat
     2221 cactaatttg gctgaggagc aattattaca tattcaaaga ttaacgcgta ttggtgaatc
     2281 actttcttca gaaaccgact tagataaaat atttgatatg attctggaag aaggaatttc
     2341 ctttacccga gccgatgctg ctacaattta tacggttaat gaggatgcca ccaaacttca
     2401 gtttgaaatt gtgtataatg ctaccttaaa tttacgcaag ggaggaacgc ggggacccgt
     2461 aaactggaaa agcattgacc tttacgatga aaaaggcaat ccggtagata acaaaatcgt
     2521 taccaatgtt tatcatcaca aacaaagctt atgtttcgct gatgtataca aagcggaagg
     2581 ttacgatgtg agcggaacta ttaagaccga tagagaaaat aactatcgct gtaagtcaat
     2641 gctaactata cccttaaaaa atcatgagga tactgtttta ggtgtgattc aattcatcaa
     2701 tgctatggat gaagaaggaa acatcgtttc ttttaccgat gagcataaag caatgctttc
     2761 ttcgcttgct tcgcaggcag ctattgctct ttccaataaa aaactgattg aaaatttgga
     2821 agaactatta aatcaattta tccgttccat tgccggtgcc attgaaagaa agtccaaata
     2881 ctcttccaat catataactc gggtagcaat gctgacggaa atgatagcca ctaaaattaa
     2941 cgaaaaggga gaaaaatatt tcccgggtcg caaattcaac gaaaacgaac taaaagaact
     3001 ttctatggct ggctggatgc acgatgttgg taaaattgtc actcccgaag caattatgga
     3061 taaaagcaca aaattggaaa caatttgtga ccgcatagag ctggtaaaat tgcgtttgga
     3121 aaaactggaa ctattgctaa cctacttgca gttaaagcta aaggaagatg aatttgctgc
     3181 ttttatccat caaaatatag acagcgaact ggagcccacg ggttttcgta attatcttgc
     3241 taatataacc aacttcctta tcaatgtaaa tgttggcaaa gaatttgttg ataaagccga
     3301 tttagaaaaa gtggaaaaac tcggaaagat aaattttgaa tatgaaggaa agcgttactt
     3361 tatttttacg gaagacgaaa aaaagaatat gcagataagt ggcagaggaa catttactcc
     3421 tgaagaaatg aaaattatgc aagatcatgt tgccatcacc tgggaaatgc tttctcagct
     3481 atctttcccc aaaaagtatg aaaatgtagc tttctatgca gctactcatc atgaagcgct
     3541 caatggaaag ggctatccta atggctatac agcagataaa ttacctttgc agtcccgtat
     3601 tttagctgta gcagatatct ttgaagcatt aaccgcttcc gatagaccct ataaaacagc
     3661 caagacctta agcgagtcat taaatattat gggttatatg gttaaggatg gtcatctgga
     3721 taaagacctg gtaaatttct ttttggatag cggactttac aaggaatatg ccgatcgttt
     3781 tatgaataag gaatatattg atgaggtaga tattgattcc ataaaagcca tatacactaa
     3841 tcaatgaccg ttcccttttt aaataagccg cttctatttt tagcaccttt agctggctac
     3901 agcgattccg cttttcgcca aatttgtaaa aattggggag ctgaggtctt atacagtgaa
     3961 atggttagtg ctgatggtat aaccagaaat tccgccaaga ccttacaata tgttcagttt
     4021 gatgagtcag agcacccttt cggcatccaa atttttggca gtaatccact cataatggca
     4081 aaagcggttg aggtgttatt acctttaaaa cccgatttta tggatattaa tatgggctgt
     4141 ccggtgaaaa aagttgtccg caagggagct ggttcagctt taatgcagac cccttcttta
     4201 gctggaaaaa tagttaaaga agttaaaaat gctctcttag gaaggatacc tttaagtgtt
     4261 aaatttcgta gcggatggaa tttccaaaat cagaattatt tacaatttgg attgatgttg
     4321 caggaatgcg gaaccgattt tctctgtttg catcccagaa cttcaagcca gatgttttcg
     4381 ggctccagca attgggaaca gataaaaatc ctgaaacaaa atgtccaaat tccggttgtt
     4441 ggaaatggag atattaaaac tccggaagac gcacaaagaa tgataacaga gaccaattgt
     4501 gacggaataa tgattggtag aggtgcttta gggaaaccct ggctttttgc gcaaagtcgc
     4561 cagttgattt ctaccggaac ttacaatcct gtaacccaaa aagaaatcgt gaagaccatt
     4621 tttgcccatc tggataaagc attgctgtat aaatcggaag atgttgttgt gagagaaatg
     4681 cgtgcacatc tttgctttta tactaaaggc attttaggag gagctgaatt acgcaatcaa
     4741 ataaatcata cagaaaacat tagcgaactg aaagcaatca tcctgaaggg attttcgcat
     4801 ataggatagt gagggctgag agctaagggc agagagatga aggatacttg caagtcagga
     4861 gtcgttagcg ctttgataac tttttggttg tgagataact tttttagcgc ttatggctcg
     4921 tgacggcata ttgacaggat ttaacgaatt tatcggattt aatggaagtt atacgaattt
     4981 acaattgtta atttgtcatc tttttctcgg tgctttcagt attctcggtg ttctcggtgt
     5041 aaaaaaatct gcgtaatctg caagatactg gaaacttctt gacactttgc tcaggtttag
     5101 aaatagaaac cacagggtat taccatacct gaataagatt aaaaaaacag ggagaaaaaa
     5161 tgaaatattt tagaaaactt gtaggagaaa agtgttatct ttcccccgtt tctatggatg
     5221 atgtggaaaa atacacggaa tggctgaatg atatggaaat agggcaattt gtaacactat
     5281 ctcatatagt tctggatata gaaaaggaaa ctgaactgtt gcgtaaattg atgcgggagg
     5341 attatatttt tgccattgtg gaaaaagata ctaataaggt tatcggaaat tgtggaattc
     5401 atcaggtctc acaagttcac agaaatgctt ctctgggaat ttttattggg gaaaagacct
     5461 tttggaatca gggtattgga actgaggcaa cgagtttgct tctggatttt gctttcaaca
     5521 ttttgaattt gcataatgtt tatctttccg tgatgtccta taacaagcgt gctattcgtt
     5581 gttatgaaaa aatcgggttt aaaaaagtgg gagtacaaag agaatttatg tttgtttcag
     5641 gacaatatca cgatgttttt atttacgata tactggcaag cgaattcacc agtccctatg
     5701 tgaaaaagat ttatgactat agcatcagtg atgaagcggg acgcagtaaa ataagcattg
     5761 tttaaatgtt acccaaaata aaaggacagg aaaatgcctt agagctttta gataaggcaa
     5821 ttgaaaatga tcgtatagct caggcatatc ttttttacgg aagtgatgga gttggtaaat
     5881 ttaccactgc cctctatttc ggaatggcag ttaattgttt agctaccagt gaattaaaac
     5941 cctgtggcga gtgtgatgcc tgtcataaat ttttgcagtt ggagcatcct gatttcgttt
     6001 atctctttcc tactcccaat ttgaatctca gtgcagaggg agagattaag aatacggacg
     6061 ctcaaaaatc gtatgaagca tatttgcaga ataaaattaa cactccttgg aaggactttt
     6121 ttttcaaaga agctacggaa atccgtaaag aaagcattac gctattgaac aaacgcctgg
     6181 aactatccat ccatgaagca aaatacagaa tagtaattat tgaagatgca gatatgatga
     6241 ataatgccac tgccaatgct tttctaaaaa ccttggaaga acctccagaa cgaactatta
     6301 taattctcat tactgaacgc ttgccattaa ttttaccgac aatactttcc cgcactcaac
     6361 ctgtttattt taagccctta acaaggggtg taattgagga aatcctaatc aacgaatttg
     6421 aaatcaattc cgctttagcc aggtctgctt ccagaatttg tgccggtaac ttgaaaactg
     6481 ccattagaat tgcttcggaa agtgaatccc tttccagtaa ttgggctttt gaaatctttt
     6541 ccctggcagc agaagaaaat gatttgggtt atttggaatt ggcagaaagg tataaaaaac
     6601 agcaaacaat agaccaggta attgatttac taaaatatat ccggattatt gccagtgact
     6661 taggttccct ttctgccaat cctgaaagcg aaattaccaa tatagataaa atagatttct
     6721 tgaaaccaat cgcagaagca aatttaggaa tagatgaccg gctgtatgat ttcctgcttt
     6781 ttctggaaga cctcaatcgt aaagttgagg gttatgccaa tttaaattta gtactgacca
     6841 acctctattt aaactgtaaa catttgctga aaccataaat ggaagctaaa ggcactcatc
     6901 ggactttggc aaaaaacatc agtgtgatgt ccattggtgt attcatcagc cgcattttcg
     6961 gtttaattag agaccaggtg atggcttatt tctttggcac aacttatttg aatgatgcct
     7021 ttaatgtggg ctacaatatt cccaatttat taagacgcct ttttggagaa ggagcacttt
     7081 ccacagcttt tgttcctctc tataatgata ttaagattaa gcagggtaag gaaaaacaga
     7141 tagaatttgc tttgaacctg ctgagcgttt taacttttat tctttgtatc ttaacaattt
     7201 taggaatagc tcttgccccg ttgattgtga aatgtcttta tccaggttta gcatctgaaa
     7261 cgaaagttct ggctataaag cttacgcgga ttatttttcc ctacctgttt ttcattggct
     7321 tatcttctac ttttattgcc attttgaact cgcataacta tttttttatg acgggacttt
     7381 cttccgcatt actcaatatc gggatgattg ccacggttct tattccccat tttgtgttaa
     7441 aagtgagtgg agaggattta attgtttggg ctggtggggg agttttagta gggggttttt
     7501 tacaaactgt aatcaatctg ccttatctga aaagaattgg ttaccgttgg gcgatttatc
     7561 taaagtttgg ttcggaggca ttgtctgttc tctggaagcg ttttattccc agtatgatag
     7621 gaattggaat tcgggaaata aacttaattg ccgattcgtt gatggcatct tttttaccaa
     7681 ctggatcaat tacggcatta aattttggca atcgtttaat gcaattaccc ttaggtattt
     7741 ttgccatttc cacgggaaca gctgttcttc ctttttattc acgctgtgtt tccactgaaa
     7801 attatcaaga gctttcagaa agtattcgtt tttcgggttt gaacatagct tacattatgc
     7861 tgccggtaac gactattatt ttagctttgg gtgaggactt tgtacgcatt ctatttgaaa
     7921 gtggagcttt tcaggaagac gctgttttga tgacttccca ggcgttaata ttttattcct
     7981 tgggtttaat tttttatggc ttgaatcaaa caattactcc tgtattttat gcttataaag
     8041 ataccaaaac acctgtgaaa attgctgcgg gaatggtagc tttaaatata accctgaatt
     8101 ttattctgat tcagtttatg gctcatcggg gtttagcttt atctacttct ataactgctt
     8161 gtgtaaattt ctttatactg cttttcctga tccgcaaaaa gatgccggaa atatcttttt
     8221 cggggattgc cataaacatt atcaaatccc ttttgatttg ttgtttctta taccttttgc
     8281 tctatataag ctgtaatctt tttcctgtaa gcggcagaat ttctttacta ataagagatg
     8341 ccatactttc tataattact cttgggctgt tttatctcac cggtatcttg ataaaaattg
     8401 attatcttaa tcaggcaggc aaaaaattat gccagcgttt tctgaagaaa tagaaacaaa
     8461 acttgctttg cttcctgaac aaccaggtgt ctatctctgg aaagataaag acggaactat
     8521 catctacatc ggcaaggcat taaacctgaa gcataggata aagaattatc tgaatcccac
     8581 tggcaaagac cttaaaacag aacatctgat aaagaatatt gccgatttgg attacatcat
     8641 taccaatagc gaagccgatg cctttatttt ggaagcaact ttagttaagc gttaccgtcc
     8701 caaatataat attatgctga aagacgataa acgctatcct ttcgtaaaaa taacggttaa
     8761 tgagcccttt cccagaatct ttattacgcg tgagttgctg aaagataatg ccaaatattt
     8821 tggtccttac acagaagttc gctatttaag aaatacttta cgcgatttgg aatggatttt
     8881 tcctttgcgt agctgcaata gaaatattcc taaaataaaa tataaacaac cctgtatgaa
     8941 ttttcagctg ggtaaatgtc ctgctccctg catcggaaac atcagtcaga aggactatgc
     9001 caagaccgta aatcagctct tgcggttttt caatggacgc tatacagaac ttttagatga
     9061 actgagaaca gaaatgcaaa atgcttctgc agaactccgt tttgaggaag caggaaaatt
     9121 ccgtgaccgc attattgcca ttgaacgcat tcaaaaaaga caatccgttt tcaatgtgga
     9181 aggcggaaac attgatatta tcggctttta tagggaagaa aaaagagctg tctgtgttat
     9241 tctgaaaatg aaaaacggag ctattattca tcaggaaaat tatccgctta caaatttgga
     9301 ttatgaagaa ccggatagta tcctggcaag ctttttacaa ctttactatg ctgatagaga
     9361 agaaatgccg gaagaagttc tgttgccttt tgcacctaaa gatatggata aactgaattc
     9421 ctggctgggc aataaattgc agatacccca aagaggagaa aaaaccaagc tgattgcaat
     9481 ggcaaaaacc aatgccttta acattgtaga agaaagcaaa ctggcacatt taagaaaagc
     9541 caatcggact attttccctg ttcaggaact gaaagaggcc ttaaaattgc ctaaactacc
     9601 acggaaaatc gtttgtatgg atatttctac catccaggga agtgatactg tttcttctgc
     9661 tgtctttttt gaaaacggga aaagcaaaaa gaaatactat cgccatttca ttattcgcag
     9721 cattgaaacc caaaatgact atgccgctat gcaggaaaca atggctcgtt ttttaacgga
     9781 aacccaaaaa gaaccccaaa tgaaaccgga cctcatcgtt attgatggag gaaaaggaca
     9841 gctttcagcc agtgaacaaa tattacgagc ttccggaaat aacgatatca gtataattgc
     9901 cttagccaaa agagcagagg aagtttttgt cccgggaaat gaacaatcca tcattttacc
     9961 gcgttcatct tccgctttac gattattgat caatatccga gatgaagcac atcgtttcgc
    10021 tatctcttta catcgcaaaa gacgctcccg cagaacattg gaaagtgaat tggaaagtat
    10081 ttccggtatc ggagaaaaaa ataaatttct gctgttgaaa gaactgggca gcgtggaaaa
    10141 tattgccaac gccaataggg aaactctgtc taaaataaaa ggtataggac ctaaaatcgc
    10201 agagaagata tataaccatt ttcacagcac ataaaattcc ttcccttttt tattacgctt
    10261 cttaactgca ttattgaagc attgcttacc caatactaat cccatccgta atgggtaagc
    10321 aatgggtaag caatgctatt aaggaagaag ttatgtggaa tacataattt attgcaaatc
    10381 aaggtggttg caaggtgaag agtgaaa
//
LOCUS       DAOPFL010000060        10324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_7929_length_10324_cov_86.485344, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000060 DAOPFL010000000
VERSION     DAOPFL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10324
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7929_length_10324_cov_86.485344"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLG69_05285"
     CDS             complement(<1..296)
                     /locus_tag="PLG69_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HPI42958.1"
                     /translation="MFWKVIAAIFCSPVFTLILCIIFLLLNILDGHSTYLVLKPDKYH
                     REKNPLARWVFQKLKIPAGIIIFKTILMSILILAIAYFTAWEPLTVNIAMLIADL"
     gene            complement(336..4550)
                     /locus_tag="PLG69_05290"
     CDS             complement(336..4550)
                     /locus_tag="PLG69_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPI42959.1"
                     /translation="MKNFWSIFKLCKFWFLLLFLCLGIFVFAEITPGQLIFKTTIPVE
                     IKGTQTGLSAFDNFLSQKGVTQIKPIKGMHQPTYYLASVSEVPPAEVLEQLSFPGIQY
                     VQPNYLRKLHSTPNDPLYSQQYHYISSIPAAWNYTVGSNYVKIGIVDSGMLIHHPDLW
                     QNLAINTKEIPDNGIDDDHNGYIDDWCGWDFTDAPEMAGNALGDFLDQDNDVEDENFH
                     GTHVSGIAGAVGNNGIGVVGVCWNVSILPVRAGFRTLDNQGFLQDDDVSAAIIYATDN
                     GCNVINMSWGDPNYSAIIADACEYAYNKGVVLVASAGNDAGPGLSYPAKLSTVISVGS
                     INIAKQLSTFSSYGDDLDLVALGERVLSTYKLTTDEQYFYMDGTSMSAAFVTGAVALL
                     LSLHPGLSPAEVKARLISATDDLAPVGFDIRTGHGLLNVKKLLDNLDPPFLEITSPQD
                     QLGISGSVPIIGSVYGSDFANYTVAYKKEKDQNDTNWKDVAEHIETLTKYSQPVHNDT
                     LAVFYIPNNFPEGKYLLRLQYEKTLHNMNRYNIYRTVIVDRTPPVLRTETISYFTRWT
                     KLNLEYYIAANFDEMVNAQLRITASDGLIYNVYSSLADSIQIWKLPSSIPEGMIDVQF
                     IATNIAGLTFVSDIFTNFLNIHYYLIPQYGYTKEIIGPPQKPLLRKRDYNGNGIAEYI
                     AMEIQASGYGSVKAFEPHPGGHIVTNDFGDSFWPLDIGSTYTTGSKSELLLLRGENAE
                     MWLTPIGGNYPSNCNDSLVIALDTGIAGGVMADYDNDGKTEILLVKNLPAERVIQAYK
                     RVSSGALAPRNTISNPTSTYSRNNFVPTIIVDNFDGDNYPDILCADTDGDVMIFEIIN
                     ATQHQLCWTTRLPIGNTYSLTAGDFNGDGHKDFFVAGYVTDIVNQNLNFWLGEGFRNI
                     GNNNYTSMGSIMFNEVSSMNSITSDDLDQDGCDEVILAITPNLYVLKYINGKFVPIFY
                     DVSSNNYQVSTWEDENGIIRILGNVKTVGDNPALAEWTPEEPFTGPPTPANLTVKPLN
                     ESSIKVSWTSTGAPEYALYRKDEEGNVTRNILTGVTSFTDTNLQQGKVYKYAIASCDP
                     SYTPSESNRGPWIEGIPLPKPELVQITFAGQKELRLFFNQEMPGAIINPACYYVSNGM
                     GNPISANSISANSGVQLRFRNNFPAIDSLFVLELRNIYGISGVEPETPFYYFAYIEDI
                     IPPEITGVKVLPSKQEITIQFSEEISPSSALYPANYNLHCPKNDPDNKIVSVSHSVDT
                     VTIKFAEKLKFSNYAYYLEVNNITDLAGNLISPQGNLVRFSIQNLDNLENLNVYPNPV
                     TSKHSPQVTFINFPVNKKGKIAIYSSSGQLVFQDDIGPFSVENNNITYTWNLRNNNQR
                     PVSSGIYFYVVEMGGKIKRGKLAIIK"
     gene            complement(4563..5465)
                     /locus_tag="PLG69_05295"
     CDS             complement(4563..5465)
                     /locus_tag="PLG69_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPI42960.1"
                     /translation="MKHTIITLCIMLIGGMCFAGIDENAGTYGYKFLNVPYGPVSLSL
                     AGRGVFSIDNPGSFLLQPAASCMNDQHLLGITHNMWLADTQANMIAYSFAQRKSHFGI
                     AMRNLDYGEIENRDDMGFLIGNYHPLDIDVTANYAHRVTPSIYAGANLGILYQKLNTA
                     SSLALHTDLGLCWLPPVKDAKITLVGRNLGIANHTDEERVKLPVCFELDINKGFSVAE
                     QHLILGGSVIQTLDEDLKGNVNLECELFQLLTLRGGYLLGYDAQDFSAGFGVKYKNIS
                     VDYGYGAFNSELNDVHSFGVTYRF"
     gene            complement(5580..8717)
                     /locus_tag="PLG69_05300"
     CDS             complement(5580..8717)
                     /locus_tag="PLG69_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI42961.1"
                     /translation="MPCLIWKRITLLITIVFVGSLSALVPPVPMYKNPPIAWQATRLE
                     GSFFAEGTLKTGKRNLPNNILVLRVQFSDVSFRDQAGYPDNLVHDDVFFNRWMIHLKD
                     FFLEASHLQYELDYYIFPQVLTMPHSLSFYGADGDNDIDVDLPQILPDILALIDEEVD
                     FSQYGGIIIFHSGTGQESDINSIRPNEIWSTFLTRKKLQKYFDPENDLYAGYPTDDGI
                     YLTNIIIVPEDEFQDYFPSADEENAANYLFSMYGVLAHQLGHLLGLPTLYDNDDSNGI
                     SQGIGNWGLMGTGLWNGSGYVPAQLCAWSRYFLGWETPLVLFQNSDNNSVDYFLNHSP
                     EAIRLYKVPISTTEYFLIENRQQNPDGSLDPLSNLPSYSFKLLPAGEQDYYEDNPLTP
                     EDESLLPYFNFMENTYLGSEWDFFLPGLGGPIPDNSPFPVDGSGILIWHIDENVINAN
                     FTANFDRNYVNSNALHKGVDLEEADGIQHLDTAAYNIYKWGSPYDSFRAGNNNYFGNQ
                     YHNGMLSLPTSESYYGGIPLELTDISGSGNQMTFSVNFGWRLTTSYQGINPINACAID
                     FDGDGKDELFFPLPNGKIYIWKNEELLSNYPIQLPFYIPYNYTWDGESIYLPLQRDEV
                     CCLYKLNSAEHYYAFRSNAYSWLSHPVDIGENLALPLKKNADDNAFAIKLYNKTDMTV
                     ASLADYSGKFIANLIHYDNQLSVVYQNAEGEYWLSEIDLSNYDKKEIKLPIPADSIIV
                     AVFKAPIADTENLIIQCPNSVYCLQGANIVDGFPYVHNLVATSDSSFIAPLTFADVDG
                     NGSLDILIGGESDFAIIDYSGKLMNLENQNPEPHTDFISAGIYAMDIDNDNSPEIIGN
                     FKYNRLNIWEHNYRPKIGYPVSFAERSRTLPFVSQASDNNWYIYCATDNGLLFRNKLE
                     NKPQITPACNWICEYGNLRRSASYTKMELPNQYQTDELFVPGQVYIYPNPLKSIYRQK
                     IQLNVMTSRDAELELSIFDISGTLVYRQKGYAKAYLKNLDIFDIPADKLCSGVYIAII
                     KSSTSSKRLKFAIEK"
     gene            complement(8999..9205)
                     /locus_tag="PLG69_05305"
     CDS             complement(8999..9205)
                     /locus_tag="PLG69_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42962.1"
                     /translation="MGKAKSFAAKVAHDNSTEGKVICPVCNTEIKRIKLITNKKKNGG
                     WTPQKEYAKICKCNEADILAGKNL"
     gene            complement(9218..9781)
                     /gene="efp"
                     /locus_tag="PLG69_05310"
     CDS             complement(9218..9781)
                     /gene="efp"
                     /locus_tag="PLG69_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPI42963.1"
                     /translation="MATMADIRNGMVIEFKDDLYEVIEFLHVKPGKGPAFLRSKLKNI
                     RTGRVLENTFRESDTFNEVRIERTKKEYLYRDGDFLVLMDSDNYEQMHIEASLLGDKE
                     NLLTENMEVIVSGTADGKVVGIELPTTVVQTIAECEPNLKGNTASGSGKVAYTETGLR
                     LMVPFFVEPGDKIKIDTRTGEYIERAN"
BASE COUNT         3338 a         2083 c         1814 g         3089 t
ORIGIN      
        1 aggtctgcaa taagcattgc aatattaacg gttaacggtt cccaggcagt aaaataagca
       61 atagccaaaa tcagaatact catcaaaatg gtcttgaaaa taattatacc ggcggggatt
      121 ttcagttttt ggaaaaccca gcgggcaagg ggattttttt ctcggtgata tttatccggt
      181 ttcagcacca aataagtaga atgcccatcc agaatgttca gcagaaggaa aattatgcac
      241 aaaataaggg taaacactgg agaacaaaat atggcagcaa ttaccttcca aaacatataa
      301 tcctaattct gtgtaagcat tatttactac aatatttatt taatgatggc aagtttaccg
      361 cgtttgattt tgcctcccat ttccactaca tagaaataaa ttccactgga aacaggacgc
      421 tgattgttat tccgtaaatt ccaggtgtaa gtaatattat tgttttctac actaaagggt
      481 cctatatcat cttgaaaaac aagttgtccg ctactgctgt agatggcaat tttccctttt
      541 ttatttaccg gaaagttgat aaaggtaact tgcggtgaat gtttggaagt aacaggattg
      601 ggataaacat tcaagttttc cagattatcc aggttttgaa tgctaaagcg aactaaatta
      661 ccttgaggtg aaatcaagtt tcctgccaga tcggttatgt tattcacttc caaataataa
      721 gcataattgc taaatttcag cttttctgca aacttaattg ttaccgtatc cacagaatga
      781 gaaacagaaa caattttatt gtcaggatcg tttttagggc aatgtaaatt ataattagca
      841 ggatacaaag ccgacgaagg tgatatttct tcactgaact ggatagtaat ttcctgtttg
      901 gaaggcaaaa ccttaactcc ggtaatttcg ggaggaatta tatcttctat gtaggcaaaa
      961 taatagaaag gggtttctgg ttccactcca ctgattccat atatatttct aagttctaaa
     1021 acaaagaggg aatcaatagc aggaaaatta ttcctaaatc gcaattggac gccagaattg
     1081 gcggaaatac tattagcgga aataggattg cccattccgt tgctcacata ataacaggca
     1141 ggattgataa tagctcccgg catttcttga ttgaagaaca agcgtaattc tttttgtccc
     1201 gcaaaagtaa tttgcaccaa ttcaggttta ggcaaaggaa ttccttcaat ccagggtcct
     1261 ctattacttt cacttggtgt ataagaaggg tcacaggaag caatggcata cttatatact
     1321 ttgccctgtt gcaaattagt atcggtaaaa ctagtaacac cagttaaaat attcctggtt
     1381 acatttcctt cctcatcttt tctatataaa gcatattccg gagcaccggt agaggtccag
     1441 gaaaccttta tagagctttc atttaagggt ttcacagtta aattggcagg tgtgggaggt
     1501 cctgtaaacg gttcttcagg agtccattct gccaaagcgg gattatctcc taccgttttt
     1561 acattaccca gaatgcgaat aatcccgttt tcatcttccc aagtgctaac ctgataatta
     1621 ttagagctta catcatagaa aataggaacg aatttgccat taatatattt caaaacatat
     1681 aaattggggg taatagctaa aataacttca tcacagccat cctgatccaa gtcgtcactg
     1741 gtaatggaat tcatagagga gacctcattg aacataatgc tgcccataga ggtataattg
     1801 ttattaccta tattccgaaa tccctctcct aaccagaaat tgagattttg attaacaata
     1861 tcggtaacat aaccagcaac aaagaaatcc ttgtgtccat cgccattaaa atcaccggcg
     1921 gtaagagaat atgtatttcc aataggaagt cgggttgtcc agcataattg atgttgtgtc
     1981 gcattaataa tttcaaaaat cattacatca ccatcagtgt cggcacataa aatatcggga
     2041 tagttatcac catcaaagtt atctacaatg attgtaggaa caaagttatt acggctataa
     2101 gtagaagtgg ggttgctaat agtatttctt ggcgctaaag cacctgagga aactctttta
     2161 tatgcttgaa taacccgttc ggcaggtaaa tttttaacca agaggatttc ggttttgcca
     2221 tcattatcat aatctgccat cactcctccc gcaattcctg tatccaaagc aattactaag
     2281 ctgtcattac agttactggg ataatttccc cctataggag tcagccacat ttcagcattt
     2341 tcgcctctca acaaaagcaa ctcactttta gaacctgtag tataagtgga accgatatca
     2401 aggggccaaa aagaatcccc aaaatcattt gttacaatat gtcctccggg gtggggttca
     2461 aatgctttta cagaaccata accacttgcc tgaatttcca tagcaatata ttcagctatg
     2521 ccattgccgt tataatccct tttccgcaac aacggctttt ggggaggtcc tataatttct
     2581 ttagtatacc catattgagg aattagataa taatgaatgt tcaaaaaatt agtaaagata
     2641 tcagaaacaa aggttaaacc agcaatattg gtagcaataa attggacatc aatcattcct
     2701 tccggaatag aggaaggtaa tttccaaatc tgaatgctat ccgcaagaga agaataaaca
     2761 ttatagatta gcccatctga agcggttatt ctgagctgag cattaaccat ttcatcaaaa
     2821 ttagctgcaa tataatattc cagatttagc tttgtccaac gggtaaaata ggagatagtt
     2881 tccgttctta aaactggagg agtgcgatct acaataactg ttctataaat attatagcgg
     2941 ttcatattgt gcaaggtttt ttcgtattgc agtcgcagga gatattttcc ttcggggaaa
     3001 ttattcggaa tataaaatac agccagagta tcgttatgaa ctggctgact atattttgtt
     3061 agcgtttcaa tatgttctgc aacatctttc cagttagtgt cattttggtc tttttctttc
     3121 ttataggcaa cagtataatt cgcaaaatca gaaccataga cagaacctat aataggaacc
     3181 gaaccactaa ttcctaattg atcctgcgga ctggtaattt ctaaaaaagg agggtctaaa
     3241 ttatccagca gtttcttaac attcaacaaa ccatgaccgg ttcttatatc aaagccgaca
     3301 ggagctaaat catccgtagc agaaattaaa cgagctttaa cttccgcagg actcaaacca
     3361 ggatgcaaag ataggagtaa tgccacagct ccagttacaa aagctgcact catagaggtt
     3421 ccatccatat agaaatattg ttcatcagta gtaagtttat aagtagatag aactctttct
     3481 cccaatgcta cgaggtctaa atcatctcca taactggaaa aagtggaaag ctgcttggca
     3541 atattgattg aaccgacaga aataactgta gaaagcttgg caggatagct taaaccgggt
     3601 cctgcatcat ttcctgccga ggcaactaat acaactcctt tgttataagc atattcacag
     3661 gcgtcagcta tgatagcaga ataattggga tcaccccaac tcatattaat cacattacac
     3721 ccgttatctg tggcataaat aatagcagcg gaaacatcat catcttgtaa aaagccctga
     3781 ttatcaagtg ttctaaaacc agctctaaca ggtaaaatac ttacattcca acagacccca
     3841 acaactccta ttccgttatt tccaactgcg cctgcaatgc cagaaacatg ggttccgtga
     3901 aaattttcat cttccacatc attatcctga tctaagaagt cccctaaagc attacctgcc
     3961 atttcgggtg catcagtaaa atcccatcca caccaatcat ctatataacc attatgatca
     4021 tcatcaattc cgttatcggg aatttccttg gtattgatag caaggttttg ccacaggtcg
     4081 ggatggtgaa ttaacattcc tgaatccaca ataccgattt taacataatt actgccaacc
     4141 gtataattcc aggcagcggg aatgctgctg atataatgat actgttgact gtataatgga
     4201 tcgttgggag ttgagtgcaa ttttctcaaa taattgggct gaacatattg aatacccgga
     4261 aaacttaatt gttccaagac ctcagcagga ggcacctcag aaacacttgc cagataataa
     4321 gtgggctgat gcataccctt aatgggcttt atctgcgtaa ctcctttttg gctaagaaaa
     4381 ttgtcaaaag cagataatcc ggtttgggtt cctttaattt caacgggaat agtggtttta
     4441 aaaataagct gaccaggagt aatttcggca aaaacaaaaa tacccaagca taaaaacagg
     4501 agaagaaacc agaatttgca cagcttaaat atgctccaaa agttcttcac ttttccctct
     4561 atctaaaatc tgtaagttac accaaaagag tgaacatcgt tcaattcact attgaaagca
     4621 ccgtagccat aatccacaga gatattttta tatttcactc caaagccggc actaaaatcc
     4681 tgagcatcat agcccaaaag ataaccacct cttaaagtaa gtagttgaaa gagttcacat
     4741 tctaaattaa cattgccttt aaggtcttcg tctaaggtct gaattaccga accgcctaag
     4801 attaaatgct gttctgcaac agaaaatcct ttgtttatat ccaattcaaa acaaaccggc
     4861 aatttcactc tttcttcatc cgtatgatta gcaattccca aatttcttcc taccaaagtt
     4921 attttagcat ctttaacggg aggaagccag cacaaaccaa ggtccgtatg caaagctaaa
     4981 ctgctggcag tattcagttt ttggtataaa attcccaaat tagctcctgc ataaatagag
     5041 ggcgttacac gatgagcata atttgccgta acatcaatat ccaaaggatg ataattaccg
     5101 attaaaaaac ccatatcgtc cctgttttca atttccccat aatccaaatt acgcatagct
     5161 atcccgaaat ggctttttcg ttgagcaaaa gaataagcaa tcatatttgc ctgggtatct
     5221 gccagccaca tattatgtgt aatgcctaaa agatgttgat cattcataca agaagctgct
     5281 ggttgcaaca aaaaggaacc cggattatca atactgaaca cacctcttcc tgctaaggac
     5341 aaagatacag gtccgtaggg aacattcaaa aatttatagc cgtaagttcc ggcattttca
     5401 tcaatgccgg caaaacacat tcccccaatc agcattatgc ataaggtaat tattgtatgt
     5461 ttcataattt tactctctct tatatcatat tttgaatgaa aattcctttg tcattcctga
     5521 gcaagcggta aaccaaacaa gttctgacaa aatattcgca tttatgtagg gaaactatat
     5581 tatttttcaa tagcaaactt gagtcgcttg ctgcttgttg aggatttaat aatagcaatg
     5641 taaaccccac tgcaaagttt atctgcagga atatcaaaaa tatccaggtt tttaagatat
     5701 gccttagcat aacctttttg acgataaacc aaggttccac taatatcaaa aatgcttagc
     5761 tccaattctg catcgcgact ggtcataaca ttaagctgta ttttttgtcg gtaaatggat
     5821 tttaagggat tggggtagat atatacctgt cccggcacaa aaagttcatc ggtctgatat
     5881 tgattaggca attccatttt agtataagaa gcactgcgtc ttaaattacc gtattcgcaa
     5941 atccaattgc aagcaggagt tatctggggc ttattttcca gtttatttct aaacagcaag
     6001 ccattatcgg tggcacaata aatataccaa ttattatctg aagcttggga aacaaaaggc
     6061 aaggttctgc tgcgttcagc aaaagaaacc ggataaccta ttttgggacg gtaattatgc
     6121 tcccaaatat taagccggtt atacttaaaa ttccctatta tttctggact gttatcatta
     6181 tctatgtcca tagcgtatat tcctgcactg ataaaatccg tatgcggttc cggattttgg
     6241 ttttccagat tcatcaattt tccggaatag tcaataatgg caaaatcgct ttcgcctccg
     6301 atcaagatat ccaaagaacc attgccatca acatccgcaa aagttagagg agcaataaaa
     6361 ctgctgtccg atgtcgcaac aagattgtgc acataaggaa aaccatctac aatatttgcg
     6421 ccttgcaaac aataaacact attaggacat tggataatta aattctccgt atccgctatt
     6481 ggtgccttaa aaaccgctac aataatagaa tcagcaggaa taggcaattt tatttccttt
     6541 ttgtcgtagt tgcttaaatc aatctcgctc agccaatatt caccttcagc attttggtac
     6601 acaacggaaa gctgattatc ataatggatc agattggcaa taaatttacc gctataatcc
     6661 gccaaagaag ctaccgtcat atcggttttg ttataaagtt taatggcaaa ggcattatcg
     6721 tcggcatttt tttttagggg cagagccaaa ttttctccta tatccacagg atgacttaac
     6781 cagctataag cattagacct gaaagcataa taatgttctg cactatttaa tttatataaa
     6841 cagcatactt catctctctg cagtggcaga taaatgcttt caccatccca agtgtaattg
     6901 taaggtatat aaaaaggcaa ttggatagga tagttactta gcaattcttc atttttccag
     6961 atatatattt tgccattcgg cagaggaaaa aataattcat cttttccatc accgtcaaaa
     7021 tcaatagcac aagcattgat aggattgatt ccctgataag aagtagttaa tcgccagcca
     7081 aaattcacgg aaaaagtcat ttgatttccg ctgccagaaa tatcggttag ttctaaagga
     7141 atccctccat aatagctttc cgaagtaggc aaggaaagca ttccgttatg atactgatta
     7201 ccgaaataat tgttattacc ggctcggaaa ctgtcataag gacttcccca tttataaata
     7261 ttataggcag cagtatccag atgctgaatt ccatctgctt cttctaagtc aacaccttta
     7321 tgtagcgcat tggaatttac ataattccga tcaaaattag ccgtgaaatt ggcattaatt
     7381 acattttcat ctatatgcca gataagaatt cccgatccat ctactggaaa aggactgtta
     7441 tccggaatcg gacctcccaa tccgggaaga aaaaaatccc actcactgcc taaataggta
     7501 ttttccataa agttgaaata gggtaatagg gattcgtctt caggagttaa aggattatct
     7561 tcataataat cctgctcacc tgcaggaaga agtttgaaac tataactggg aaggttacta
     7621 agagggtcta aactaccatc gggattttgt tgccggtttt caattaaaaa atattccgta
     7681 gtagaaattg gcactttgta aagacggatt gcttccggag aatgattgag aaaataatcc
     7741 acagagttat tatcgctatt ctgaaagagc acaaggggtg tttcccaacc taaaaaataa
     7801 cgggaccagg cacaaagctg agccggaaca taaccacttc cattccataa accagttccc
     7861 attaaacccc agttgccaat tccctggctg ataccattac tatcatcatt atcatataaa
     7921 gtaggtaaac ctaataaatg acccaattga tgagccagca ctccatacat actaaagagg
     7981 taattagcag cattttcttc atctgcggaa ggaaagtaat cctgaaattc atcttcaggc
     8041 actataataa tgttagttaa ataaattccg tcatcagtcg gataaccggc atacaaatca
     8101 ttttctggat caaaatactt ttgcagtttc ttccgggtta aaaaagtgct ccagatttcg
     8161 ttggggcgga tagaattgat atcggattcc tgtccggttc cggaatgaaa aataatgata
     8221 cctccatatt gactaaaatc aacttcctca tctattaaag ccaaaatatc aggtaaaatt
     8281 tgaggtaaat ctacatctat atcattatct ccatcagctc cgtaaaagga aagggaatga
     8341 ggcattgtca acacttgcgg aaatatataa tagtccagtt catattgtaa atgacttgct
     8401 tccagaaaaa aatccttcaa atgaatcatc caacgattaa aaaaaacatc gtcgtgaacc
     8461 aaattatcag gatatcctgc ttgatcccga aaagatacat cactgaactg cacccttaaa
     8521 accagaatgt tattaggtag atttcttttg ccggttttta atgttccttc cgcaaaaaat
     8581 gatccttcca aacgcgtggc ttgccaggca ataggtggat ttttatacat tggaacagga
     8641 ggaaccaaag ctgataatga accaacaaaa acaatggtta tcagcaatgt tatccgcttc
     8701 cagataaggc aaggcatttt aatcctcccc caaaataata ttatcgttaa aatgaaaatt
     8761 cccttgtcat tcctgcaaaa gcagaaatcc agacaagttc tgacaaaata actgccattt
     8821 ccaatataaa aattatataa tattgtgtag ctaaaaaatc cgctaccgaa ctaatgcaag
     8881 tggatagctt tatagacctt ttatggtgct tacataaata gtcaagtaaa aaacggcgaa
     8941 agtatatcgt cttctttggc aagtgtatat tgctttctct taagataact gtatatcttt
     9001 aaagattttt gccagctaat atatcagctt cattgcactt gcaaattttg gcatattcct
     9061 tctgaggagt ccagccgcca ttcttctttt tattagtaat aagcttaatc ctttttattt
     9121 ccgtattgca tacagggcag attactttcc cttctgtgga gttatcatgc gctactttag
     9181 cggcaaaact ttttgctttt cccattttta tcctctttta atttgctctt tctatatatt
     9241 cacccgtgcg ggtgtcaatt ttaattttat ctcccggttc tacaaagaaa ggaaccatca
     9301 agcgtaaacc tgtttccgta taggcaactt taccacttcc ggaagcagtg tttcctttca
     9361 aattgggctc gcattcagct attgtttgaa ccacagttgt tggcagttct attcccacaa
     9421 cttttccatc agcagttccc gatacgataa cctccatatt ttccgtaagc agattttctt
     9481 tatctcccaa taacgatgcc tctatgtgca tctgttcata gttatcgcta tccatcagca
     9541 ccaaaaaatc tccatctcta tacaaatatt cctttttagt gcgttcaata cgaacttcat
     9601 tgaaggtgtc actttcccga aaagtatttt ccaaaaccct tcccgtgcgt atatttttca
     9661 gctttgaacg cagaaaagca ggtcctttac ctggttttac atggagaaat tctatcacct
     9721 cgtataggtc atctttgaat tcaatgacca ttccgtttct aatatcagcc atcgtagcca
     9781 tattggtttc ctttccctta ttcttatgat gacaagcttt tggagagggg gattttggca
     9841 agggaatttt ttctttcaca aagagaaaag aaaaaagaga aaattgaagt tagaatgaaa
     9901 aagtaagagg gttgagaggg ttgagaaaat ttagaaggtt tagaaggttg agaaggttta
     9961 gaaggttgag atttcatcaa agcccggagg gcgacacaat catagcgatg ggtgcgtaag
    10021 cccctcggga aaaaaggttg agaaggttga gaaggtttag aagattgaga tttcatcaaa
    10081 gcccggaggg cgacacaatg atagcgatgg gtgcgtaagc ccctcgaaaa atgtaagccc
    10141 cgacttcttt tttcttttct tcttcccatt ttccaaaagc ccgcagggcg acacaatcat
    10201 agcgatggtg gcataagccc ctcgtaaaaa agtaagaaac aaccatctat ctttttccac
    10261 tgttccccag tataaatatc ctgaaaatac ctttcatccc attcctccca gagctaataa
    10321 atct
//
LOCUS       DAOPFL010000061        10063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_8238_length_10063_cov_104.843325, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000061 DAOPFL010000000
VERSION     DAOPFL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8238_length_10063_cov_104.843325"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLG69_05315"
     CDS             <1..753
                     /locus_tag="PLG69_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI42964.1"
                     /translation="NGILTTRPFRSPHHTISDVALIGGGAFPKPGEVSLSHRGVLFLD
                     ELPEFKRVVLEVLRQPLEDGIVTISRAASSLTFPAEFMLIASMNPCPCGYFGSNIPNH
                     QCNCEWGSILRYRSRISGPLLDRIDIHVEVPSVSYADLASLPTGDKSVDIRARVNKAR
                     AIQHDRFKGTGIFNNSQMNSKQLRKFCILDDASNALLQNAIDKMGYSARVFDRILKVS
                     RTIADLEGRKDIISDDISEAIQYRTLDRKYWS"
     gene            1102..1350
                     /locus_tag="PLG69_05320"
     CDS             1102..1350
                     /locus_tag="PLG69_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPI42965.1"
                     /translation="MNTDQLMTLAVNKNGFVFDPENGISYTVNETGLFVLQQLQSGAT
                     QDEIIKCLTEEYEVTEKNAISDVDHFMAMLKALNLIED"
     gene            1355..2422
                     /locus_tag="PLG69_05325"
     CDS             1355..2422
                     /locus_tag="PLG69_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPI42966.1"
                     /translation="MLQLNDLQISIAVSGLKTGDNPQPGIPVIRSLRAAGFKGKIIGF
                     VYDAMESGIYLDDIADEIYQMPYPSTGAESFLTRLNYIHSKSKIDVIIPTLDSEIPLY
                     IRLHKELKALGIHTFICTEKQFNLRDKSKLFQYFTSKDVAVPKTVLLNSVAEINKAIQ
                     DIEFPVFIKGRLYEAYRANNIIEAQKYFWELQAKWGLPVIMQELMEGDEFNVVIVGDG
                     KGNCLGMVPQRKLVITDKGKGFGGVVVNNPALEKFARKIIQILSWRGPCELEIIKDKE
                     GAFHLLEINPRFPAWVRLAEGSGQNQPAATVLLALGEIIEELPPFKPGVLFIRHSEDI
                     ISDINLLGEISVNGELIRMHN"
     gene            2436..3785
                     /locus_tag="PLG69_05330"
     CDS             2436..3785
                     /locus_tag="PLG69_05330"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPI42967.1"
                     /translation="MKKHYTAPYIERNSSGNLNKYAHTTVVRHQDNIDGVPIKILVQK
                     YGTPLFVFSEKQIRRTYRRLLETIRIHYPKVDIVWSYKTNYLAAICNIFHQEGAPAEV
                     VSRMEYEKARSNGISGNRIYFNGPSKRKQDLQIAIKEGAYIHIDHLEELYQIENIAEE
                     LAIKPKVAIRVNMDTGISPMWTRFGFNYENGEAYRTVQRLISGNKVQLVGLHTHIGTF
                     ILDPNAYYWAATKLLNLAQTIKDNFGIVLDYIDMGGGFASNNTLIDQYTPGELASPSF
                     EQYAEAIGTAFNASHYVKEYHPRLILETGRVLIDESGYLIASVLGKKNLPTGERAVIL
                     DAGVNINITAWWYKQRVLPTKPFPGTYQNTVFYGPLCMNIDVIRSAMPFPDLYYGDTV
                     IISPVGAYNVTQWMQFIEYRPNVCLINETGEVDVIRSQEDLSDLTSREYIPDHLRQI"
     gene            3818..5968
                     /locus_tag="PLG69_05335"
     CDS             3818..5968
                     /locus_tag="PLG69_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HPI42968.1"
                     /translation="MFLAIPDSYATILFSDSVLLGLMLLTITLLSPVVGLSGLFSLIV
                     AILAVRILGFEHWESSSGITAFNSLLAGMMVGYYYPMQIISSSTINYLCFLLLVSLFT
                     LLLYLFSSYLFQQFFRLPSMSLSFSIVAILLWYYFARKGYLSNYPFDKILLFSPTLKL
                     PEFWRLFFISLGSIFFTPEVSAGILVSIALLIITRIGFGLALLGWTLNYILMRLAGVQ
                     PGSGVFYSGFNAILIMYAVAGIYLLPGKSSILIGLIATGIGFLFTALFNVVYYYYNAF
                     TGLYTPLAVPVFAFPFNIVVLLVIFSLRLRLKVDKPVMNDYGVFNPELALQTYQERYK
                     RFSHLGIPQFAMPLNGLWTITQGNNGIYTHKLDWAYAWDFEMLNSEGRNYDKENNNLN
                     DYFSFSKPVLASAAGCVVKICEGVADNPIGQVNTKENWGNYVCLSHSYGLYSFYAHLK
                     QGSIRVKVGDYIQKGDCIAQLGNSGRSAVPHLHFQVQLGIEPGSPTRLSHLINYKLIK
                     EDKTLEFIGSGVPKEKETLSALVAEPHLQSILALQQQNEQQLKVTRGKKQSTELWKVH
                     LDLWGNFTIKSTKHTDLSFSVYDGIYNALSLKGNHKSALAAFALLISRLPYSEKQEIY
                     LYDEPAFSVIISPALRHIVLLFSPLFPLFSACVHSKIKVNSDFIEVESDITYRILSIP
                     YRNRTGNVSIEKYKGLAALELKKGKKTLLKAETI"
     gene            5987..6589
                     /locus_tag="PLG69_05340"
     CDS             5987..6589
                     /locus_tag="PLG69_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42969.1"
                     /translation="MKRIILPCLLILAAGFLLGQDKASITDSYNAETARNYTRALQIM
                     EDLSANDPNDEFYILRIAWLQYLLGSYNESMKNYQKSNNLYPSLDAQTGILNCQLALG
                     MWNEAHNQAEHILQNFPQNIAVLSKAAYSTYMLQNYRATADYYQRIVNIFPWDMESRG
                     YLVNNLYLSKDIENARQHYQILKKYAPDSAMAKEYSKIFK"
     gene            6594..7796
                     /locus_tag="PLG69_05345"
     CDS             6594..7796
                     /locus_tag="PLG69_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42970.1"
                     /translation="MRYFWLILLIIPCLLFSEDIVTLPFNLISVAYNNMLTGNYEIAE
                     KQYQQLTILYPQERSGWEGLLWSQNAQGKFHKSLKTFSNAKTKLSELDGIYNYYAFAL
                     YRQNRLPEARYYYKQALDNMPANPLANQVSCEGLAYTYLALDNYPKAQKYFSKAAFIS
                     GRQMSAIKPSFNSTVYYKVPGTEKNAYGFRQSAKYKCAELKLNYEYFQLDNAFFRDLY
                     KADFTYQFIPLEVGINGSYLSGEDARVYPAWQLGMELCPKLYPGKIVLNPELFVSFSH
                     YPRFDVQQISLHPQVLWRDFSFSYALHSAFMDNEPSETDSVHFAQQFCLTKSLPYSFE
                     LGFHYGAGNDTWIIDNSGVIIDTFNQNGSYYGISLGKEFFKHLYLYGYYQKWDSEDLF
                     YFSLSGYY"
     gene            7796..>10063
                     /locus_tag="PLG69_05350"
     CDS             7796..>10063
                     /locus_tag="PLG69_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42971.1"
                     /translation="MRRIFFFLLLIVLLASCNLRENLLLPPEISAADYQTGNTIKVYS
                     DYLIKAANDDSYLMLHKESIADELIHLGDEIVFRKVKTFAMRDSLGFQNNAEAKSNTY
                     QFGVIRSDTIIDLIASINMAEIYTEIKPSSDPFYLVSFNYYLQANPINPTYYNKRRAY
                     FPISATGEYALLSIPEEDNPTLQHNGMDNFYAVLLNNTGAQVAVNFPAAYTSMAGNIT
                     IRLKDNLTDYNKLTAYYPNAAISYPVVELQTEKAIGNCLAYLRILNAGKGFFSRQWIH
                     LSENSVYSWSENDPVTETSNWWIDETGLYSFLKGSGEYFLLSPLENQQEISVPLDGSV
                     NSVFLQQVWFDLRSISLPETQMKVNIAPDISSILADYFQNNPYSFNSPVQAFVINFYK
                     DSELIATLPENNWIEFGFFTSQTENNKNRLFSVCCNNLQDIITYKSPGTSYDANHYVQ
                     VNSYIYSGITSSAAYLYGCLQTAPSQLKIPYYKNRQYLQTENAIISWWNADKTDYDYL
                     ILNLKPALSNHPWLKGEPFYISAASSLADFCFYMHNEKQSSLPSGFYLALPVFNLQEN
                     YLLFSTFPYSRLKNYLQGTANCYVQKNWLNIYPEFPGMIINCNINYTNPLKLRTYSTM
                     NFFWNDLIFYTYGNAPQETNTIFSFYKTNTLADPYRILSNQYNLSYTSAVYQVSSDSE
                     ENFALFQPVLFFPRTAKGQNLLFYEKTEPFYRLYSFYESASYDPWYFYIDNGFNGIAL
                     ANSGSYASFVDNNPHN"
BASE COUNT         3085 a         1790 c         1858 g         3330 t
ORIGIN      
        1 aatggtattt taaccaccag accttttcgc tcgcctcatc atacgattag tgatgttgct
       61 ttaattggag gaggtgcttt tcctaaaccg ggagaggttt ctctttcgca taggggagtt
      121 ttatttttgg atgagcttcc ggaattcaaa agagtggttt tggaagtttt gcgtcaaccg
      181 cttgaagatg ggatagttac aatttcccga gcagcatcca gtttaacttt tccagcggaa
      241 tttatgttga ttgccagtat gaatccttgt ccttgcggat attttggttc caatattccc
      301 aatcatcaat gcaactgtga atggggttca attttgcgtt atcggagcag aatttccgga
      361 cctcttttag atagaataga tatccatgtg gaagttcctt ctgtcagcta tgcggatttg
      421 gcttctttgc ctacaggaga taagtcagtt gatatcagag ccagagtaaa taaagccaga
      481 gccattcaac atgaccgttt taaagggacg ggaattttta ataacagcca aatgaattcc
      541 aagcagttac gcaagttttg cattttggat gatgcaagta atgcactgct gcaaaatgcg
      601 atagataaaa tgggctattc ggcaagggtc tttgatagaa tactaaaggt ttccagaact
      661 attgcagacc tggaaggacg caaagatata atctctgacg atatcagcga agcaattcaa
      721 tatcgcactc tggatagaaa atactggagc tgaattgatg agatagaaaa cgcattagaa
      781 agattttaac ggatgaacag attattatag cagagggcag aggacagaga gaaaaaaaac
      841 tatgattttc tgtagagtga atccataatt gggatagtaa acaattttgg cattagcttt
      901 atgagctata aaaaaaatta ttcacaatat ataggtaaga taggagcttt tgtaactccc
      961 ctttataact tgctaattac gatatttcct taaagttatc ctttttttcc cgatcaaatt
     1021 ggcttgatat ttgcataata tacatactaa ataagcggga tacaaattat catatcctga
     1081 aaataaactg ctgagggaaa aatgaacaca gaccaactaa tgacccttgc tgtaaacaaa
     1141 aatggctttg tatttgatcc tgaaaacggg ataagctaca ctgtaaacga aacaggcctc
     1201 tttgtcttac agcaattgca atcaggagct acccaagacg aaattataaa atgtttaaca
     1261 gaagagtatg aggtaactga aaaaaatgct attagtgatg tagatcattt tatggcaatg
     1321 cttaaagcgt tgaatttgat agaggactaa tagtatgctg caattaaatg atttacagat
     1381 cagcattgct gtatctggtc ttaaaacagg tgacaatccc caacctggaa ttccggtaat
     1441 tcgttctctt agagccgctg gttttaaagg taaaataatc ggctttgttt atgatgcaat
     1501 ggaatctgga atatatttag atgatatagc agacgagatt tatcaaatgc cttatccttc
     1561 taccggtgca gaatcatttt taacacgctt gaattatatc cattccaaaa gtaaaattga
     1621 tgtgattatt ccaactttgg attctgagat tccgctttat atccgcttac acaaggagct
     1681 taaagcactt ggtattcata catttatctg cacagaaaag caatttaacc tgcgtgataa
     1741 gagcaaacta tttcaatatt ttacttccaa agatgttgct gttcctaaga ccgtcctatt
     1801 aaatagtgtt gcagaaataa acaaagcaat tcaagatata gagtttccgg tttttattaa
     1861 gggtcgtctc tacgaagcat atagagccaa taatattata gaagcgcaaa agtatttttg
     1921 ggaactgcag gcaaaatggg gcttaccggt tattatgcaa gaacttatgg aaggtgatga
     1981 atttaatgtt gttattgtag gggatggcaa aggaaactgc ctggggatgg ttccacagcg
     2041 taaactggtt ataacagata agggtaaagg ttttggaggg gtagtagtaa acaatcctgc
     2101 tctggaaaag tttgctcgta aaataatcca gattctttct tggcgtggac cctgcgaact
     2161 ggaaattatc aaggataaag agggtgcttt tcatttactg gaaattaatc ctcgttttcc
     2221 tgcctgggtt cgtttagcgg aaggtagtgg ccaaaatcaa cctgcagcaa cagttttact
     2281 tgctttgggt gaaataattg aggaattgcc tccttttaaa cctggagttc tttttatccg
     2341 tcattcagaa gatataatta gtgacataaa ccttctgggt gagatttcag ttaatggtga
     2401 actgataaga atgcataatt aaaagaggaa ataaaatgaa aaaacattat actgctccct
     2461 acattgaacg taatagctct ggaaatctta ataaatatgc ccatacaacg gttgtccgac
     2521 atcaagataa catagatgga gttccgatca aaattttagt acagaagtat ggaacaccct
     2581 tgtttgtttt ttccgaaaaa cagattagac gaacctaccg gcgtttgctg gaaacaatcc
     2641 gtattcatta tcctaaagta gatattgttt ggagctataa aactaattat ttggctgcta
     2701 tctgtaatat ttttcatcag gaaggtgccc cggcagaagt agttagtaga atggaatatg
     2761 aaaaagccag gagtaatggc atttctggaa acaggatata ttttaatggc ccttctaaac
     2821 gcaagcaaga tttgcaaata gctattaagg aaggtgccta tatacatatt gatcacttag
     2881 aagaactgta tcaaattgaa aatattgccg aagaacttgc cataaaaccc aaagttgcta
     2941 ttcgcgtaaa tatggataca gggattagtc caatgtggac tcgttttggc tttaattatg
     3001 aaaatgggga agcgtatcga acagtgcagc gtcttatttc cggaaacaaa gttcagctgg
     3061 ttggtttgca tacccatatt ggcactttta ttttagatcc taatgcttat tattgggcag
     3121 ctaccaaact tctaaattta gctcaaacta ttaaggataa ttttggtatt gtattggatt
     3181 atatagatat gggaggaggt tttgcttcga ataatactct aattgatcaa tacactccgg
     3241 gagaattggc ttcaccatct tttgaacagt atgctgaggc aataggaact gcttttaatg
     3301 cttctcatta tgtgaaagaa tatcaccccc gtttaatttt ggaaactgga agggtgttaa
     3361 ttgatgaatc gggttatctt attgccagtg ttcttggcaa aaagaatctt cccacaggtg
     3421 agcgagctgt aattttagat gcaggagtga atataaacat cactgcttgg tggtataaac
     3481 aacgcgtttt acctaccaaa ccatttccag gaacatatca aaacaccgtt ttttatggtc
     3541 ctctgtgtat gaatattgat gtaattagaa gtgcaatgcc tttccctgat ctttattatg
     3601 gcgatacagt tattatttca cctgttggtg cttataatgt aactcagtgg atgcagttta
     3661 tagaatatcg tcccaatgtc tgcctaataa acgaaaccgg tgaagtagat gttattcgtt
     3721 cccaagaaga tctttcagat ctaacttccc gagaatatat tccagatcac ttacggcaaa
     3781 tatgaactgg tcacgatttt tccggtctgt ggggtctgtg tttttagcta ttccagatag
     3841 ctatgccaca attttgtttt cggattcagt gcttttagga ttgatgttgc ttacgattac
     3901 tttactatct cctgttgtag gtttatctgg tttattttct ttaattgtgg caattttggc
     3961 agtaagaatt ttgggctttg agcattggga atcaagtagt gggataactg ctttcaattc
     4021 acttttagca ggtatgatgg ttggttatta ctatccgatg caaattatca gcagttcaac
     4081 tataaattat ctttgttttc tgctgttagt ttctcttttc actcttttac tatacttgtt
     4141 tagcagctat ctttttcaac aattcttccg tttaccttca atgagcttat ctttttccat
     4201 agtagctata cttttatggt attattttgc tcgcaaaggg tatttatcca attatccatt
     4261 tgataaaata ctgcttttca gtccaacact caagttacct gaattttggc gtttattctt
     4321 tatttctttg ggttctatct tttttactcc ggaagtttct gcaggcatct tagtttctat
     4381 agctttacta ataattacgc gcatcggatt tggcttagca cttttaggat ggacgctaaa
     4441 ttatatatta atgcgtttgg cgggtgtgca accaggaagc ggagttttct attcaggttt
     4501 taatgctatt ttaattatgt atgctgttgc gggaatatat cttttgccag gtaaaagttc
     4561 cattttgatt gggttaattg caaccggaat tggcttttta tttactgcct tattcaatgt
     4621 tgtatattat tactacaatg cttttactgg cttatatacc cctctggcgg ttccagtttt
     4681 tgcttttccg ttcaatatag ttgttttact agtaattttc agtttgcgtt tacggctgaa
     4741 agtggataaa cctgtaatga atgattatgg tgttttcaat cccgaattgg ctttgcagac
     4801 ctatcaggaa cgctataaaa ggtttagcca tttaggtatc cctcaatttg ctatgcctct
     4861 aaatggtttg tggacaatca ctcaagggaa caatggaatc tatactcata aattggattg
     4921 ggcttatgct tgggattttg aaatgctgaa tagtgaaggg agaaactacg ataaagaaaa
     4981 caataattta aatgactatt tttctttcag taaaccggtt cttgcttcag cggcaggttg
     5041 tgtagttaaa atttgcgaag gagtagctga taatcctatt ggccaggtta atactaagga
     5101 gaattggggt aattatgttt gcctttctca cagctacgga ctttactctt tttatgcaca
     5161 tctcaaacag ggtagcatca gagttaaagt tggcgattat attcaaaaag gggattgtat
     5221 tgctcaatta ggaaattcag gacgctcagc agttccgcat ttacattttc aagttcaatt
     5281 gggaattgaa cccggcagtc caacccgtct atcacattta attaactaca aacttatcaa
     5341 ggaagataaa accctcgaat ttatcggtag tggtgttccc aaggaaaaag aaactctttc
     5401 tgcattagta gcagaacccc atttacaaag tattttagct ttacaacagc aaaacgaaca
     5461 gcagctaaaa gttacccgcg gtaaaaaaca aagcaccgaa ttatggaaag tacatcttga
     5521 tctttggggc aatttcacca ttaaatctac taaacatacc gacctgagct tcagcgttta
     5581 tgatggaatt tataatgctc tttctttaaa agggaatcat aaaagtgctc ttgcagcttt
     5641 tgctttgtta atttcccgtc taccatacag tgaaaaacag gaaatctact tatatgatga
     5701 accggctttt tctgtaatta tcagtcctgc gttacggcat atagtattac ttttcagccc
     5761 cttatttcct cttttttctg cttgtgtaca cagtaaaata aaggtcaatt cagattttat
     5821 tgaagtagaa agcgatatca cataccgtat tttgagtatt ccatatagaa acagaacagg
     5881 aaatgtaagt atagaaaaat ataagggatt agcggctttg gaactaaaga agggtaaaaa
     5941 aaccttacta aaagccgaaa caatttaata aataaggagt tatataatga aacgaattat
     6001 tctaccctgt ctccttatct tggctgcagg gtttcttctg gggcaggata aggcatctat
     6061 tactgattct tataatgctg agaccgccag aaattatacg cgtgcactac aaattatgga
     6121 agacctaagt gcaaacgatc ccaatgacga attttacatt ttacgcattg cctggttgca
     6181 gtatctctta gggagttata atgaatcaat gaaaaactat cagaaatcca ataacctata
     6241 tccttccttg gatgcacaaa ctggtattct gaattgtcaa ttagctttag ggatgtggaa
     6301 tgaagcccat aatcaagcag agcatattct gcagaatttt cctcagaata tagctgttct
     6361 cagtaaagct gcttattcca cctatatgct tcaaaattat agagctacag cagattatta
     6421 tcaaagaatt gttaatatat tcccttggga tatggaatcg cgtggttatc tggtaaacaa
     6481 tctgtatctt tccaaggata tagaaaatgc tcgccagcat tatcaaatct taaagaaata
     6541 tgctcccgat tctgctatgg caaaggaata ttctaagata ttcaaatagc taaatgcgtt
     6601 atttttggct gattcttctt attatcccct gtctgctttt ttcagaagat attgttacct
     6661 tgccatttaa ccttatttcc gttgcctata ataatatgct taccggaaac tatgaaatag
     6721 ccgaaaaaca atatcaacaa ttaaccattt tatatccaca agaaagaagc ggttgggaag
     6781 gattgctttg gtctcaaaat gctcagggca aatttcataa atctctgaaa accttttcaa
     6841 atgccaaaac aaaactgtcg gaattagatg gaatttataa ctattatgcc tttgccttat
     6901 atcggcaaaa ccgtttgcct gaagccaggt attactataa acaagcttta gataatatgc
     6961 ctgcaaatcc tttggcgaat caagtttcct gtgaaggttt agcttacacc tatctggctt
     7021 tggataatta tcctaaagca caaaaatatt tttctaaggc agcttttatt agcggcaggc
     7081 aaatgtccgc tataaaaccg tcttttaata gcactgtcta ttacaaagtt ccaggaacag
     7141 aaaaaaatgc ctatggtttt aggcaatctg ccaaatataa atgtgcagag cttaagctaa
     7201 attatgaata ttttcaattg gataatgcct ttttccggga tctatataaa gcagatttta
     7261 cttaccaatt cattcctttg gaagtaggca tcaatggatc ttatctttct ggagaagatg
     7321 ccagagttta tcctgcatgg cagttaggaa tggaattatg tccaaaactg tatccgggaa
     7381 aaattgtttt gaacccagag ctttttgtct ctttcagtca ttatccacgt tttgatgttc
     7441 agcagataag tttgcatcct caagttcttt ggcgtgattt ttctttttct tatgctttgc
     7501 atagtgcgtt tatggataat gaaccctcag aaacggatag cgttcatttt gctcaacagt
     7561 tttgcctaac aaaatctctt ccgtatagtt ttgagcttgg ttttcattat ggagcaggaa
     7621 acgatacttg gataatagat aattctggag tgattataga taccttcaat caaaatggtt
     7681 cttattatgg aatctcctta ggtaaggaat ttttcaagca tctctattta tatggttatt
     7741 atcaaaagtg ggatagcgaa gaccttttct atttttcgct ttcaggttat tattaatgcg
     7801 tcgtatcttt tttttcttgt tattaattgt tttgcttgct tcctgtaact tacgggaaaa
     7861 tttattgtta cccccagaaa tttctgcagc agattatcaa acggggaaca ctatcaaggt
     7921 ctattccgat tatttaatta aagccgcaaa tgacgattcc tatttaatgc tacacaaaga
     7981 atccattgcc gatgaattaa ttcacttagg tgacgaaatt gtttttcgta aagtgaaaac
     8041 atttgctatg cgagattcct tgggctttca aaataatgcc gaagcaaaaa gtaataccta
     8101 tcaatttggc gttatacgtt ccgatactat aatagacctt attgcttcta tcaatatggc
     8161 cgaaatatac actgaaataa aaccgagttc agatccattt tatttagtta gtttcaatta
     8221 ttatctgcaa gccaatccta taaatcctac atattataat aagcgcagag cttattttcc
     8281 tatatctgct acaggtgaat atgctctatt gtctatacct gaagaggata atcctaccct
     8341 gcaacataac ggtatggata atttctatgc tgttttgctt aataacacgg gagcccaggt
     8401 agcggtaaat tttcctgctg catacacatc tatggctgga aatataacta ttaggttgaa
     8461 agataattta accgattaca ataaattaac tgcctactac cccaatgcgg ctatttctta
     8521 tcctgttgta gagttacaaa ctgaaaaagc aatcggcaac tgccttgctt acttaagaat
     8581 attaaatgca ggaaagggat tttttagcag acagtggatt catctatcag aaaattctgt
     8641 atacagctgg tctgaaaacg atcctgtgac cgaaacttcc aactggtgga tagatgaaac
     8701 aggtctatac agttttttaa aaggcagcgg tgaatatttc ctgttaagcc ctttggaaaa
     8761 tcaacaggaa atatccgtcc ccttagatgg aagcgttaat agtgtatttt tacaacaggt
     8821 ctggttcgat ttacgctcaa tttctttacc ggaaacccag atgaaagtta atatcgctcc
     8881 cgatatttct tccatactgg ccgattactt tcaaaataat ccctattctt tcaattcccc
     8941 tgtgcaggca ttcgtaatca atttttataa agattctgaa cttatagcta ccctacctga
     9001 aaataactgg atcgaatttg gctttttcac tagccaaaca gagaataata aaaaccgcct
     9061 atttagtgtt tgctgtaata accttcagga tattattact tataaaagcc caggaacaag
     9121 ctatgatgcc aatcattatg tccaagttaa ttcctatatc tattctggga taacatcttc
     9181 tgcagcatat ctttatggct gtttacaaac agctccctcg cagttgaaaa ttccttatta
     9241 taaaaatcgc caatacctgc aaaccgaaaa tgccattatt agttggtgga atgccgataa
     9301 aactgattat gattatctta ttctaaattt aaaacctgct ttatccaatc atccttggct
     9361 gaaaggagaa ccgttttata tcagcgcagc gagttcatta gccgattttt gtttctatat
     9421 gcataatgaa aagcaaagct ctttgccttc aggtttttat cttgctttac ctgttttcaa
     9481 cctgcaagaa aattatcttc tattttccac ttttccttat tcgcgcctaa aaaattatct
     9541 gcagggaaca gcaaattgtt atgtccaaaa gaactggctg aatatctatc ccgaatttcc
     9601 cgggatgatt attaattgca acataaacta taccaatccg ttaaaactga gaacttattc
     9661 cacaatgaac ttcttctgga atgatctcat tttttatact tatggcaatg cccctcaaga
     9721 aacaaacaca attttcagct tttataaaac taatacactt gcagatccct atcgcattct
     9781 ttccaatcaa tataacctca gttacacttc tgctgtatat caggtaagtt cagattccga
     9841 agaaaatttt gctctctttc agccagtgct ctttttccca cgcactgcaa aaggacagaa
     9901 tctgctcttt tatgaaaaaa cagaaccctt ctatcgactt tattcttttt atgaaagcgc
     9961 ttcttacgat ccctggtatt tttatattga taatggattt aatggtatcg ctttagctaa
    10021 tagcggttcc tatgcctcct ttgtagataa taaccctcat aat
//
LOCUS       DAOPFL010000062         9763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_8603_length_9763_cov_69.965595, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000062 DAOPFL010000000
VERSION     DAOPFL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8603_length_9763_cov_69.965595"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /gene="secD"
                     /locus_tag="PLG69_05355"
     CDS             <1..719
                     /gene="secD"
                     /locus_tag="PLG69_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPI42972.1"
                     /translation="DNVGKRLAIVLDGVVYSAPNIQERIAGGRAQITGRFTATEANEL
                     AIVLNTGNLIAPIKPVSTSIIGATLGADSIRKGTMAGLIGLILVVLFMAIYYKLCGFI
                     ADIALVLNVGFILAMLTAFGATLTLPGIAGIILTIGMAVDANVLIFERIREELELGKT
                     PRSAVEAGYKRALVTVWDSNLTTVIAGIILYYLGTGPIRGFAVTLVIGIMGSMFTAIV
                     VVRTILDNMVLTGKRTKLSV"
     gene            731..1714
                     /gene="secF"
                     /locus_tag="PLG69_05360"
     CDS             731..1714
                     /gene="secF"
                     /locus_tag="PLG69_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPI42973.1"
                     /translation="MRILKNTNIQFVKSRYWFYGISIALLLISLCGIIFKGLNWSIDF
                     TSGIAATVNLKALDPKVPAVPIENLRTVLTKNGFPEAEITYIGNPANSTFQIKIKSVG
                     KGTDLSTETRTKLLEIIQQNFPEYVQGRDLNKEVIEEIYEVGPKVGSELRSQAFWAVM
                     LALIAMIIYIWFRFEFTFGLMGILALFHDVFIIVGIFALTGREMTMQIIAALLTIVGY
                     SINDTIVIFDRIREDMKKNRKDPIQVVFNRSLNKTLSRTIITGGTTFLTSACLFLWGG
                     SVIHDFALAICLGIIFGTYSSIFVASNLVIDLNLITHKERKTMQHLTKKKL"
     gene            2483..2797
                     /locus_tag="PLG69_05365"
     CDS             2483..2797
                     /locus_tag="PLG69_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42974.1"
                     /translation="MKKLFIILITFIPISAFLCGQTILPKEKEGSIIVSNLGNTPGSI
                     SPFNISKNGFVLPLAYGLVVNLISIKRIGNYEIYSQAKAIILSVSHNDLCVNIFLTNE
                     DK"
     gene            2835..5138
                     /locus_tag="PLG69_05370"
     CDS             2835..5138
                     /locus_tag="PLG69_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPI42975.1"
                     /translation="MKKILLLIIMLLCSVALFSQYEPWLWAKQATGTNSFSAYKIAVD
                     IYGNSYVSGYFYDEVTFGNITLNSNGMCDIFIAKLDPEGNWLWASKAECPSDCFNYGI
                     AVDSNGNCYVTGWFNYEAIFGEIILNGNGEYYNEDIYIAKLDSNGNWLWAQKAGGISD
                     DCGWEIAVDSNGNCYVIGNFFESATFGTTILESSGSSDIFVAKLDNDGSWLWIKTAGG
                     EDNDFRVGIDINTDNNGNIYITGYFQGDVSFDSTTLSSKGNYFDIFVAKLDNDGNWLW
                     AKQAGGDNNDFSKEIMTDIVGNSYITGTFSGNATFGSTTLSSVGETDIFVAKLSSNGN
                     WLWAKQAGGINVDNSSSITLDSSENCYITGTFRGSATFGSTTLSSVGETDIFVAKLSS
                     NGNWLWAKQAGGINDDSSWSIDVDENHNCYITGNFYSTVSFGGIELTNLSNRAIFVVK
                     FNNPDYPNNVFIEDADTGINIRVSGGHAYKGSIENFPDIPDQDVSYTKLSFILDNSIE
                     NWTITMWTDADFGAYYQNEEWHIVDNNGGQVVFNISFAPGKGIVEIPVILDNPQTLSV
                     QLSAFTLNLNSQYGVNVKWISQSETGLNGYYIHRGTVDDLSQATIISPLIRATNTSHQ
                     QVYLYTDKDIYEPGTYYYWLAAQDYDGYVTYCGSRNVIYSIDNNQGTPELPLVTEIKS
                     IFPNPFNPTAIISYTISKPAEVNFAIYNPKGQLIRSFNLGRKDAGNYRLVWDGTDNKN
                     QFCGTGVYYIRMQAGKESFIKKAALLK"
     gene            complement(5486..6094)
                     /locus_tag="PLG69_05375"
     CDS             complement(5486..6094)
                     /locus_tag="PLG69_05375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPI42976.1"
                     /translation="MTILYQNDIEFFNTHGLFAGIDEAGRGALAGPVVAAAVVLNYNT
                     LIEDINDSKLLTPKKREELYRQIVESAIDYSIVEISPAYIDRYNILRATLLGFKNAFF
                     QLKSKPRYCLIDGRDIHSELKPYAKAIIKGDQLYASIAAASILAKVHRDKLMLAYDDI
                     YPEYGFASNKGYGTAYHAKMICSYGLSAIHRKSFKTTEVTRS"
     gene            complement(6097..7548)
                     /locus_tag="PLG69_05380"
     CDS             complement(6097..7548)
                     /locus_tag="PLG69_05380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPI42977.1"
                     /translation="MDKTYRISIVTFFSNEVPLVIDACEFSPIRKEVELELICPSEDA
                     LKYKTDNLQLTGLNNINKLPVPTPQQICFFASPASFDYFCKQENINSSYLYVHPDMAV
                     LELKGNNPFHTDLAEYGLCPCVLKEDTAEDLYEKKEKLIYFSDLSQFIENKKHNYVLL
                     STNFRFIEALINIDRENWELFHPELNYRNLRVNIGKPHSILLNAQKQLLNMARPLDDI
                     YDLAGIRFCRRNNSSAYSFFAEHYDDYMAHVDYALWVNSILYWFKQYSPLKLSKILEL
                     ACGTANMSIQFVRKGYTVFACDNSVDMLKIAARKTVKPKLYYASLTDPILGKDYQLVI
                     CMFDSINYLTQTKQIAKVLQEVSLALETGGLFIFDISTLENSMDNFAHLCDLHRYSDG
                     LMIHQAYFEYIQLIQKSSLHFFKKNILGYSYQFEQHQQRVYYTYELIEIIRNSPLKLI
                     AIHSTETKTNLYPKHLTTIDDKFSRLFFVLQKV"
     gene            complement(7683..8471)
                     /gene="pyrE"
                     /locus_tag="PLG69_05385"
     CDS             complement(7683..8471)
                     /gene="pyrE"
                     /locus_tag="PLG69_05385"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPI42978.1"
                     /translation="MEEITLITIATYTNPFEAELAKGLLEDSGFHPNLLNERIMSMYA
                     SIAGDMYKIELQVPENEEKDAKELLTGLEDSDYVTRILKDEGALLEGHFQLTSGKHSN
                     QYIEKARILQNPAAAHNLCKRLAKRLAEYEFEAIISPAYGAIALGFETAYLMGKNFVF
                     TQRKEGEMIIRNGFDLSHIWKIAIVEDILTTGGSIREVISCIQNKGLEVIAIGILIDR
                     SGGKIEFPVPLESLLCLEIPAYERDNCDACKQNIPLEIPGSSDK"
     gene            complement(8670..9215)
                     /locus_tag="PLG69_05390"
     CDS             complement(8670..9215)
                     /locus_tag="PLG69_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPI42979.1"
                     /translation="MTTEMICAGFGGQGVLTIGKFIAQAAMQEGKNVSWLPSYGPEMR
                     GGTANVSTVVSTEPIASPIVSFPDVLVALNQPSIDKFAPSIRPGGLLIYNTNMCPNGC
                     KRDDITKIAVPMNDIASEIGSMIVLNMLATGIIIGKTGIIKYETLEKDLTSFMQEKNP
                     DLLAKNLTAIKRGIEIAKQYT"
     gene            complement(9215..>9763)
                     /locus_tag="PLG69_05395"
     CDS             complement(9215..>9763)
                     /locus_tag="PLG69_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPI42980.1"
                     /translation="AHGRAPAVATGIKRARPDMHIFTYQGDGDLAAIGTAEIVHSANR
                     GEKISVFFVNNAIYGMTGGQMAPTTLPNMKTTTSPYGRNVDDIGYPIRMCELLATLVA
                     PYYIERVSLLSPADIIKAKKAVNKAIRYNKENRGFTFIEFISTCPTNWGYEPEQARQW
                     AKENMLPFFKLGVYRDKGEGVE"
BASE COUNT         3118 a         1655 c         1859 g         3131 t
ORIGIN      
        1 ctgataatgt aggaaaacgt ttagctatcg ttcttgatgg agttgtttat tctgcaccga
       61 atattcagga aagaattgcc ggtggtagag ctcaaattac aggtagattt actgctacgg
      121 aagcaaatga actagcgatt gtgctcaata caggaaattt gattgctcct ataaaaccgg
      181 tttcaacttc cataattggt gctactttag gtgcagatag tattcgtaag ggaactatgg
      241 caggtttaat cggtttaata ctcgttgtcc tatttatggc tatctattac aagttgtgtg
      301 gttttattgc cgatattgca ttggtcttaa atgtaggttt tattttagca atgttaacgg
      361 cttttggcgc tactcttact cttcctggaa ttgccggtat tattttgaca atcggtatgg
      421 ctgttgatgc taatgtgctt atttttgaac gcataagaga agaattggaa ttgggaaaaa
      481 ccccccgcag tgcagttgaa gccggttaca aaagagctct tgtaacggtt tgggattcta
      541 atctgactac tgttattgcc ggaataatcc tttattatct tggcacaggt cctatccgcg
      601 gttttgctgt aactttagtt attggtatta tgggttctat gtttacagca atagttgtgg
      661 tacgaactat tttggataat atggttttaa ccggaaaaag aacaaaactg agtgtgtaag
      721 gagtaacaaa atgagaattt tgaagaatac taatatccaa ttcgtaaaga gccgttactg
      781 gttttatggt atttccattg ctttgttact aatcagctta tgcggaataa tttttaaggg
      841 tttgaactgg agtattgact ttaccagtgg aatagctgcc accgttaatt tgaaagcatt
      901 agaccctaaa gttcctgctg ttcctattga aaatttacgc acagttctta ctaaaaacgg
      961 ttttccggaa gcagagataa cctatattgg caatccagct aattccactt ttcaaattaa
     1021 aataaaaagt gttggcaagg gaactgacct ttcaaccgaa acccgcacta agttattgga
     1081 aatcattcag caaaattttc cagaatatgt tcaaggaaga gaccttaata aagaagtgat
     1141 agaagaaatt tatgaagttg gtcctaaagt tggcagtgaa ttacgctctc aggcattttg
     1201 ggcagttatg cttgctttaa ttgcaatgat tatctatatt tggttccgct ttgaatttac
     1261 ctttggttta atgggaattc tggctctttt tcatgatgtc tttattattg taggtatttt
     1321 cgctcttacc ggaagagaaa tgacaatgca aattatagct gcactcttaa ctattgtggg
     1381 ttatagcatt aatgatacta ttgtgatctt tgatcgtatc cgtgaagata tgaagaaaaa
     1441 ccgcaaagac cctatccagg tggtattcaa tcgctctctc aacaagaccc tatcccgaac
     1501 tataattacc ggtggaacta cttttctaac ttctgcttgt ttgtttttat ggggtggaag
     1561 tgtaattcac gattttgctt tggctatctg cctgggtatc atttttggaa cctattcttc
     1621 tatctttgtt gccagtaatt tggtgattga cctgaattta attacccaca aagaaagaaa
     1681 aacaatgcaa cacctaacta agaaaaaatt gtaataatat cagatttact aacctatata
     1741 attagaaccg ctcataaatt ctgggcggtt cttttttaca cagagaacac tgagaatatt
     1801 tttacaaaga ggaaaagagt aaaagagagt gtttggacaa agagaaaaga gaaaaagaga
     1861 atgtttgaac aaagagatta agttttacaa taggaaaaca aggtgtagcc ggaggaagtt
     1921 gttcatcggc aagtaagtga aaaggtggaa aagtgaaaca gggcttggac atcgtaaaaa
     1981 aaagatgcgg aacttcagcg agcttcggct acacaaatca ggagtcgtga tcgctttaat
     2041 aaattataac ctactttcaa gcttatttat cgatgaggac tcgtgacgac atttagccac
     2101 cgaaaaagtc atttaacagt tctatgttag gaaagcgcta aatacttatg acatacaaat
     2161 ggctcttgac ttaagttagt ccactttttt aggaataaat atctatcgca atataccaat
     2221 ctaagatcaa ggtaaaaatt ccttttttct cttttctctt ataggaaaaa taattatttt
     2281 ggagtttgtg gaaattaatc aacagattat tagttatata actaattgat aaatatatta
     2341 taagctttat attgtgtcct tttatagcga gccattaaaa aggtataaaa tgaaaatagc
     2401 aaaattatga cttgacagaa catcgccata atatgctgaa attaatttat aaagttataa
     2461 tcatattata ttaggagaag taatgaaaaa gctttttatt atcttgatta catttatacc
     2521 tatttccgca tttctttgcg gtcaaacaat tctgcctaag gagaaagaag gttctataat
     2581 tgtttctaat ttgggtaata ctcccggttc aataagtcct tttaatattt ctaaaaacgg
     2641 ttttgtttta cctcttgctt atggtttagt tgttaacctg atatccataa agaggattgg
     2701 gaattatgaa atatatagtc aagctaaagc aattatttta tctgtatctc ataatgattt
     2761 atgtgttaat atatttttaa ctaatgaaga taaataacac tatcaataaa ttaacattga
     2821 gaaagaagga aattatgaaa aagatattat tactaattat aatgttacta tgttcagtag
     2881 ctctcttttc ccaatatgag ccatggcttt gggcaaaaca agccaccgga acaaattcat
     2941 tttctgcata taagattgcc gttgatatct atggaaacag ctatgttagc ggttatttct
     3001 atgatgaagt cacatttggc aatattactt taaatagtaa cggaatgtgt gatatcttta
     3061 tcgcaaagct tgacccagaa ggtaattggc tctgggcatc aaaagcagaa tgtcctagcg
     3121 attgttttaa ttacggtatt gctgttgact ccaatggtaa ctgctatgta acaggttggt
     3181 ttaattacga agctattttc ggagagataa ttcttaacgg taatggtgag tattataatg
     3241 aagacattta tattgccaaa ttagatagta atggtaattg gctttgggcg cagaaagccg
     3301 gggggatatc agatgattgt ggttgggaaa ttgctgttga ctccaatggt aactgctatg
     3361 taataggtaa tttttttgaa agtgcaacat tcggcaccac tattctagaa agtagcggaa
     3421 gttcagatat ttttgttgct aaacttgata atgatggtag ttggttatgg ataaagacag
     3481 cgggaggtga agataacgac tttcgcgtag gtattgatat taatactgat aataacggaa
     3541 atatctatat taccggttat tttcagggcg atgtttcctt cgactctacg acactaagta
     3601 gtaaaggaaa ttattttgac atatttgttg ctaaacttga taatgatggt aattggttat
     3661 gggcaaagca agctggtggt gataataatg acttcagcaa agaaataatg accgatattg
     3721 ttggaaacag ttatatcact ggaactttta gtgggaacgc tacctttggt agtacaactc
     3781 tatcaagtgt aggagaaact gatatctttg ttgccaaact tagtagtaac ggtaactggc
     3841 tctgggcaaa gcaagctggt ggaataaatg tagataacag cagtagcatt accttagatt
     3901 caagtgaaaa ttgctatatc actggaactt ttcgtgggag cgctaccttt ggtagtacaa
     3961 ctctatcaag tgtaggagaa actgatatct ttgttgccaa acttagtagt aacggtaact
     4021 ggctctgggc aaagcaagct ggtggaataa atgatgatag tagctggagt attgatgtag
     4081 atgaaaatca taactgctac attactggaa atttttattc gactgtttcc tttggtggta
     4141 ttgaattaac taatcttagt aatagagcta tctttgttgt aaaatttaat aatccagatt
     4201 atcctaataa tgtttttata gaagatgcag atacgggtat taacattaga gtatctggag
     4261 ggcatgctta caaaggcagc attgagaatt ttcccgatat tcctgatcaa gatgtatcct
     4321 atactaaatt aagttttatc cttgataaca gcattgagaa ttggactata acaatgtgga
     4381 cagatgccga ttttggtgct tactatcaga atgaagaatg gcatattgtt gataataatg
     4441 gcggtcaggt agtatttaat attagttttg ctcccggcaa aggaattgta gaaatacctg
     4501 taattcttga taaccctcaa acattatccg ttcagttatc tgcttttact ctaaatctca
     4561 attctcaata cggggtaaat gtgaagtgga taagtcaatc cgagacaggg ctaaatggct
     4621 attatatcca tcggggaaca gttgatgatt tgtcccaggc aactataatc agtcctttga
     4681 ttagagctac caatacttct catcagcagg tatatttata taccgataaa gatatttatg
     4741 aaccaggaac ttattattac tggctagcag ctcaggatta tgatggctat gttacttatt
     4801 gcggttcaag aaatgtaatt tattccattg ataataatca aggaactcct gaattacccc
     4861 tggtaactga aattaaatct atctttccca atccattcaa ccctactgct ataattagtt
     4921 acaccatcag taaacctgca gaggttaatt ttgccatcta taatcctaag ggtcaattaa
     4981 tccgtagctt taatttggga cgaaaagacg caggaaacta tagattggtg tgggatggga
     5041 cagataataa aaatcagttt tgcggaaccg gtgtatatta cattagaatg caagcaggga
     5101 aagagagttt tataaagaaa gcggcgttgc tgaaataaaa aaataaatat agcgagatga
     5161 tgagatagtt tagaaaagtt ggaatcttta ttgtgaagac ggaggacgct gttcctccgc
     5221 ctttttatat ctctcacaga taacacagat ttcacagata aaaaataagt gcacgcaaag
     5281 aatgtgtagc cggaggaacc tgatcttcca ctttgtaagt ttgacgcttt ttaattactg
     5341 agaacactga ggacactgag aatattttta caaagagaaa agagaaaaag agaatgtata
     5401 agtaaagata tctcacattc ttcatttttc atccttcatt tcctccagaa ccttttgact
     5461 taaagaacct cttgacttca agacctcaag acctcgtgac ctccgttgtt ttaaagcttt
     5521 ttctatgtat cgcagacaat ccataactac aaatcatttt agcgtgatag gcagttccat
     5581 agcccttatt agatgcaaaa ccatattcag gatagatatc atcataagca agcattagtt
     5641 tatctctatg gactttagct aaaatagagg cagcagcaat tgaggcatag agttgatcac
     5701 cttttattat tgctttggca taaggtttta gttccgagtg aatatctcta ccatcaatta
     5761 gacaatatcg gggtttgctt ttcaattgga aaaatgcatt tttaaagccc aaaagagttg
     5821 ctctcagaat attgtatcta tcaatataag cagggctaat ttctacaatg ctatagtcaa
     5881 tagcactttc tacaatttgc cgatagagtt cttcgcgttt tttaggcgta agcaacttgg
     5941 aatcgttgat atcttcaata agtgtattat agtttaagac cactgcagca gctacaacag
     6001 gaccggctaa agcaccacga ccggcttcat caattccggc aaaaagaccg tgggtgttaa
     6061 agaactctat atcgttctga taaaggatag tcataatcac accttttgca gaacgaaaaa
     6121 cagacgactg aatttatcat caatagtggt taaatgtttg ggatagaggt ttgtttttgt
     6181 ttctgtgcta tggatggcaa ttaatttcag aggtgaatta cggataatct caataagttc
     6241 ataagtataa tagacccttt gctgatgctg ttcaaactga tagctatagc ccaaaatgtt
     6301 ctttttaaag aaatgcaaag aggatttctg gattaattgg atatattcaa aatatgcctg
     6361 atgaatcatt aaaccatcac tataacggtg caaatcgcaa agatgggcaa aattatccat
     6421 actgttttcc agagtagaaa tatcaaaaat aaataagcca ccggtttcta aagctaaaga
     6481 gacctcctgc aaaactttgg ctatctgttt tgtttgagta aggtaattaa tgctgtcaaa
     6541 catacatatt accagctgat aatctttacc taaaatcgga tcggttaaag atgcgtagta
     6601 taatttaggt tttactgttt tcctggcagc aattttgagc atatcaacag agttatcaca
     6661 agcaaaaaca gtataaccct ttcgcacaaa ttgaatagac atatttgctg tgccacaggc
     6721 aagttccagt atcttagaaa gtttcaaggg agaatattgt ttaaaccaat aaagtatact
     6781 attcacccac aaagcataat ccacatgtgc catatagtca tcatagtgtt ctgcaaaaaa
     6841 ggaataggcg gaagagttat ttcttcggca aaaacgaatt cctgcgaggt cataaatatc
     6901 atccaacgga cgtgccatat ttaatagctg tttttgagca ttaagaagta tactatgggg
     6961 cttaccaata tttacccgga gattgcgata attcagttcc gggtgaaata attcccagtt
     7021 ttccctatct atatttatca gtgcttctat aaacctgaaa tttgtgctca gtaaaacata
     7081 gttgtgtttt ttattttcaa taaactgact taaatcacta aaatagatca gtttttcctt
     7141 tttttcatat aagtcctcag cagtatcttc ttttagcaca caagggcaca agccatattc
     7201 agcaaggtct gtatgaaagg gattattgcc tttaagctcc aaaactgcca tatcaggatg
     7261 gacataaaga taggaggaat ttatattttc ctgcttgcaa aaataatcaa aagaagcagg
     7321 actagcaaaa aaacaaatct gctgaggtgt tggaacagga agtttattaa tattattcaa
     7381 tcctgttaat tgcaaattat ccgttttata ttttagagca tcttcagaag gacaaattag
     7441 ttccagctcc acttctttcc tgataggaga aaattcacag gcatcaataa ctaagggaac
     7501 ttcattagag aaaaaagtaa cgattgaaat gcgatatgtt ttgtccataa tagatgctct
     7561 tgctatatag ttttcatatt ggaaatgcgg ttatgttgtt tatctttatc tggattcctg
     7621 tttgcgcagg aatgacaaag ggattactct ccggaatatg atcgcaagca ttaaaaaata
     7681 aactacttat cactgctacc aggtatttct aagggaatat tttgtttgca ggcatcacaa
     7741 ttatcccttt cataagcagg aatttcaagg cacaggagtg attccaaagg aacggggaat
     7801 tctattttac ctccgctacg atcaatcaga ataccaattg cgattacttc caaaccttta
     7861 ttttgaatac aggagataac ttcccgaata ctgccaccag ttgtgagaat atcttccact
     7921 atagctattt tccatatatg ggaaagatca aaaccattgc ggattatcat ttcaccttct
     7981 tttcgctgag taaacacaaa gttctttccc atcagataag ctgtttcaaa gccaagagca
     8041 atagctccat aagcaggact aataattgct tcaaattcat attcggctaa gcgttttgca
     8101 aggcgtttac aaaggttatg agccgcagca ggattttgca gaattctggc tttttcaata
     8161 tattgattgc tatgttttcc ggatgttaac tgaaaatgac cttccaagag agctccttca
     8221 tctttcagaa tgcgggttac atagtccgaa tcctcaagac cggtcagtag ttctttagca
     8281 tctttttctt cattttccgg aacctgcaat tctattttat acatatctcc cgcgatggat
     8341 gcatacatac tcataatccg ttcatttagc agattgggat gaaaaccgga atcctctaaa
     8401 agaccttttg ccagttctgc ttcaaaagga ttggtataag tggcaatagt aatcaaagtt
     8461 atctcttcca ttttatcctc catttatggg acgctatttt tgtctgccaa atatctttat
     8521 cattcctgct caggcaggaa accatttaaa atatacttta gaggtcttgt acctctttag
     8581 caaagaaaga atttagcaag ctaaaagtgc caggaaccga aaaaattgta aggagaaaaa
     8641 atcggctccc ggcattagga aagaactatt taagtatatt gtttagcgat ttctattccg
     8701 cgtttaatag cagtaagatt tttggcaagc aaatcaggat ttttttcttg cataaaggaa
     8761 gttaaatcct tttccagggt ttcgtatttg ataattcctg tttttccgat tataatacct
     8821 gttgccagca tattaagcac aatcatactg ccaatttcgg acgctatatc attcatcgga
     8881 accgctattt tagtaatatc atcacgctta caaccgttgg gacacatatt agtgttataa
     8941 attaaaagtc ctccaggacg aatagaagga gcaaatttat ctatagaggg ctgatttaaa
     9001 gctaccaaca catcagggaa actgacaatt ggagaagcaa taggttccgt agaaaccacc
     9061 gtagaaacat tggcagttcc acctctcatt tctggtccgt aagaaggcag ccaggaaaca
     9121 tttttccctt cttgcatagc tgcctgggca atgaatttac ctatagtaag aacaccttga
     9181 ccgccaaaac cggcacaaat catttctgta gtcattattc cactccttca cctttatcac
     9241 gataaacacc taatttgaag aaaggcaaca tattttcttt tgcccattgt cttgcttgtt
     9301 cgggttcata accccaattc gtagggcagg tgctgataaa ttctataaag gtaaaaccgc
     9361 gattttcctt attatagcgg atggctttgt taacagcttt ttttgcttta ataatatcag
     9421 ccgggcttaa tagtgagacc ctttcaatat aatagggagc taccagggtt gccaaaagtt
     9481 cacacatccg aataggataa ccgatatcat ctacatttct gccgtaagga ctggtagtag
     9541 ttttcatatt aggcaaagtt gttggagcca tttgtccacc tgtcatacca tagatagcgt
     9601 tattaacaaa gaaaacgctt atcttttctc cccgattggc agaatgaaca atttccgcag
     9661 ttccgatagc agctaaatca ccatctcctt gataggtaaa tatatgcata tcgggacggg
     9721 ctctttttat gcctgtagca acggcaggag cgcgtccatg agc
//
LOCUS       DAOPFL010000063         9689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_8719_length_9689_cov_89.019307, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000063 DAOPFL010000000
VERSION     DAOPFL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8719_length_9689_cov_89.019307"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLG69_05400"
     CDS             complement(<1..383)
                     /locus_tag="PLG69_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42981.1"
                     /translation="MKKIYILFALTLICLTPLFAGRYAGDFMMIGSGVRALGMGGAFA
                     SLADDGSGIYWNPAGLSFLTDSEVSAMHCFLYNNLASYDHITYAQPLPNDVTICLNVT
                     RLTVSDIPHFDEKYLIGTNVDQRIN"
     gene            complement(394..5475)
                     /locus_tag="PLG69_05405"
     CDS             complement(394..5475)
                     /locus_tag="PLG69_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI42982.1"
                     /translation="MPMYYINRVSNLFRSLSCRKILFFCLSFCLPALIMAEPITLVSE
                     SQDILAINFELPEYELTKVTINGQNWERIVCSDGSYLSNEGFPQLIMFSTAIAVPVDG
                     DYTFSIKSSESKTLTNINIIPSSTLVIEGEEVNYNNKPDYKAYANRELYPVSLAQKGE
                     PAFIGNRKFIPLLIYPFQYRAQSKELIVHSQISITVYISGTKNATKNWQLNPNPLDNA
                     DPPFFLNENSSKTWRLEKTRDINYSAPKNNISSINEIQIIVDKEGIYKVSYHYLMDYI
                     NLMADSLQIVMNWTPANVDPRYLELTDEYGQVPIHFVGENDGVFNENDYFEFYGDKHK
                     GDTCYMDDYTSENVYTLKLIDHYGARMAVENGGLIESNLLNYIVPDAYEDTVHFEEQL
                     VSDKLGRGWSSSTPNFYREDVWFWKKINAPNLEIVPVELQYPKDTTTRFASARICLMG
                     LTYSETLTSGQYDHEASIRLNQAMVNSHTWIGQTEKIFENQNHISNTFLRHGTNNFYI
                     ALSGNTVMTDKEQVMLDWAEIKYWREYKTDQDYIKFTKPSNRPGGLYQFEVSGFSNPH
                     ISVYKIGSSIFNSAQIEPFNINGDAPWTVTIQDSVFSEAVRYFAVTENMKKTPKAIRL
                     NFPSDLKNPDNSADVIVIAPWQFTSVEGTLQLKSLWESEGHTVKIVDVQDIYDEFNAG
                     ITGAEPIRDFLRYAYNNWSPPQLSSVILLGEGVDDTRDNSPSRIYNLIPVKKTWTYKH
                     GATASDPWYGCIVGTDIVPDISIARISIWKEQQILDYAAKATAYRNNPQTSRLWNSHL
                     TFTSGGKITDNNDIFAQQSERIIRKTIPDEYRVTRVYTSTQTVSSDYFGGTFNLKDAI
                     NSGTQYVQFMGHGGGRIWADYNLFNFNDVATLNNQTYPVVLSLACYASAFDTNGMASI
                     SESLVLQPNKGAIGTAGFTGLGYLDHDETWGMAYCDALYKHNFANIGLANIFALARAY
                     TTIYSPAAIYALINGFAYLGDPLIKLKKPIKDIPVFADNYVLNPGDTLRVNATFPTGV
                     NAGRLFIMKESGKIVNVPYDLPVFPNGNWTATYVNTNPAANNYTRKIMVAGYSNTDEY
                     IGLSQFSVGRPNIMHHHLNPPEPAWSDSVLFTAKVFSPLPVNNIICKVRTDSVSTQIN
                     WLQLPMQRSEQDSTLYVTTHYLGKYPTGKEIFFKYVMETETGITESFLKNYIVRGPDL
                     FLKDIKLEQEGNSLVLKVLGTNIGDATSITTDLKLYTGTTESNLTLFSTQNYSPLEVG
                     ELRWDTIPLAGLPNANLILEARVNTTNAFSEWHFFINTNNYIRLKVPMNYFLVDSSSS
                     THNSIDNNLQCSIPPGLVPAGSQVLFAINTLNALPPLNQPDVQNILLNAPDGLTGNQH
                     SIPYEISFLGTGITDSLGVLNGGKKLFLSFNYSATDSLTQAQESANNFKIYRYNAKFQ
                     KWILIGSFMDQSQNKVSFEVNRTGIYSIFRNMDYTPPTIDVNVEDQEFTVGGYVAGNG
                     VISLLLSDTNGIDVIDNSISLFLDGVPIPEKDYVISINLENINRIPIKYQLSLGKGIH
                     ELKVQCRDLNGRPANRDIQFTVNDKFDVINLANYPNPVLGAGGEGAALDPKNEGRTRF
                     TYVLTDGADEVTIKIYTLSGRLVKTFKNLPVGVGYHEYPRSVYGWDCKDDKGYTLANG
                     VYFYRIIAKKGNKTIEKTQKMAILN"
     gene            complement(5480..6727)
                     /gene="purD"
                     /locus_tag="PLG69_05410"
     CDS             complement(5480..6727)
                     /gene="purD"
                     /locus_tag="PLG69_05410"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPI42983.1"
                     /translation="MNILVIGSGGREHSIAECFAKSKQIKRVIVAPGNAGIALQYECA
                     NLQTQEEIYNFCVQNLIDLVFVGPEKPIADGLADYLNEKGVAVIAPVKFAAQLESSKI
                     FAKNLMQEKGIPTAAFKIAKTIPQALEHIDYFGFPAVIKNDGLAGGKGVFIVKNPAEA
                     QEALKKIHFSEKGVVVEEFLQGWEVSLFAITDGWEFKTTIFAQDHKQLYAGDKGPNTG
                     GMGAYAPVYEAEKYRKQIEEEIINPVLMAMRELGHLYKGILYCGLMITKEGPKVMEFN
                     CRLGDPEAQVVLPLLETDFVEICQAILQGEINLVELKFKDKTALGVVLASEGYPFTYN
                     KGFPLSLPADLSGIYFSGVEKGENGLVTSGGRVLCAVGMGKDLNEAREEAYSKVQQIT
                     FENRIFRQDIGLRKNTFYNKGDS"
     gene            complement(6724..7953)
                     /locus_tag="PLG69_05415"
     CDS             complement(6724..7953)
                     /locus_tag="PLG69_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HPI42984.1"
                     /translation="MNAAIITIGNEILMGKTINTNLAYLGSELAKQGISVEYALTVKD
                     EPNAIKQALQDTWGKYKIVITTGGLGPTEDDLTKSAISEFFGKKQHFDDNIWEHIQKM
                     FAFRNIPLPETNRSQAMVPDDFIPLRNDLGTAPGLFYREGDFLFFALQGVPSEMKHIF
                     ETHIKDIITQNCPDAKPLSIKTLRTYGIAESRLAELFTLSNLPEGVSLAWLPQIGRVD
                     LRFYGTDANKVEEAVKKALPVIKPFVWGYDEESPAEILLALLWKNIYTLSVAESCTGG
                     LVQKLITDIPGSSNSFLGGVIAYSNAVKKNLLNVSDTVLETEGAVSETCALQMAEGIK
                     SLTKSTCSISITGIAGPDGGTAEKPVGTVCFGFIAAEKYWSKKQFFTGDREIIRIKAA
                     DFAILSLIQHLQGRNDN"
     gene            complement(7955..9478)
                     /gene="proS"
                     /locus_tag="PLG69_05420"
     CDS             complement(7955..9478)
                     /gene="proS"
                     /locus_tag="PLG69_05420"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPI42985.1"
                     /translation="MSKNNKTAITPTREENYAEWYQQVIKAADMAENSEVRGCMVIKP
                     WGYAIWENIQRALDKMFRETGHRNAYFPIFIPKSFFEKEAEHIEGFAKECAIVTHSKL
                     EADGKGGLQLAGELTEPYIVRPTSETIIGASFAKWVNSYRDLPLLINQWANIVRWEMR
                     TRLFLRTTEFLWQEGHTVHETEKEAKEETLKMLNVYATFARDYLALPVIQGEKTESEK
                     FPGAVTTYCIEAMMQDRKALQSGTSHFLGQNFAKASEIKFQNRKGEIEYAWTTSWGVS
                     TRLIGAIIMAHSDDNGLVLPPKIAPSHIAIIPIYRDAQEQKQVMAFAEKVQNQLKDLY
                     LDDVPLYTELDTRDMTSSERNWYWIKKGIPLRLEIGPRDIIQDSVMLARRDKEPQDKQ
                     SVKISDLTNYVLQTLAEMQQGYYQRALAFKDKWTMEIDDKEEFYRFFTPKNIEQPEIH
                     GGFASSHWCGKSSCEEKIKEDLKVTIRCIPFNTKEETGNCICCGKPSTRRVIFAKAY"
BASE COUNT         2939 a         1949 c         1784 g         3017 t
ORIGIN      
        1 ttatttatcc gctggtctac attagtgccg atgagatact tttcatcaaa atggggaatg
       61 tcactaaccg ttaaacgagt aacattcaga caaatagtta catcattggg caagggttga
      121 gcataagtta tatgatcgta agaagcaaga ttattgtaga ggaaacaatg catagcggaa
      181 acctcggaat cggtaagaaa actgagaccg gcaggattcc aataaattcc agaaccatca
      241 tctgcaagag aggcaaaagc accacccata cctaaagcac gcactccaga accaatcatc
      301 ataaagtcgc cagcataacg tcctgcaaaa agtggagtta agcaaatcag ggtaagtgcg
      361 aataggatat atatcttttt cattttggtc tccctaattc aaaattgcca ttttttgggt
      421 tttttcaatg gttttattgc cttttttggc aataatccgg taaaagtaaa ccccgttagc
      481 caaagtgtaa cccttatcat ctttgcaatc ccaaccataa actgaacggg gatattcatg
      541 ataaccaact ccaaccggaa ggtttttaaa ggtctttacc aggcggccgc tgagtgtgta
      601 aatcttgata gtaacttcat ctgctccatc ggtaagaaca taggtgaaac gagttctgcc
      661 ttcatttttc gggtctaaag cagcaccttc acctccggct cctaaaacgg gattcgggta
      721 atttgctaag tttataacat caaatttgtc attaacggta aattggatat cccggtttgc
      781 aggacggccg ttcaaatcac gacattgaac ttttaattcg tgtattccct ttcctaagga
      841 taattgatat ttaataggga ttctattgat attttccaga ttgatggaga ttacatagtc
      901 cttttccgga atgggaacac catctaaaaa cagagagata gaattatcta ttacatcaat
      961 accattggta tcggaaagca agagagaaat aactccatta ccggcaacat atccgccaac
     1021 agtaaattcc tggtcttcaa cattaacatc tatggttgga ggagtataat ccatattgcg
     1081 aaaaatactg tagatacctg tgcgattcac ttcaaagctg actttatttt gactttgatc
     1141 cataaaactg cctattaaaa tccatttctg gaattttgcg ttatagcgat atattttgaa
     1201 gttattagcg ctttcctgtg cttgagttag ggaatcagta gcgctgtaat tgaaggaaag
     1261 aaaaagtttt tttcctccgt tcaaaacacc taaagaatcg gtaattccgg ttcccaagaa
     1321 acttatttcg tagggaatgg aatgttgatt tccggtaagt ccgtcaggag cattaagcaa
     1381 aatattttgg acatcaggtt gattaagagg aggtaaggca tttaatgtat ttatagcaaa
     1441 aagaacctgg ctacctgcag gaacaagacc tggaggaatt gaacattgaa ggttgttatc
     1501 tatactgttg tgagttgaac tggaactatc taccaagaaa taattcatcg gaactttcaa
     1561 gcgaatatag ttattggtat tgataaagaa atgccattca gaaaaagcat tagtagtatt
     1621 aactcttgct tccagaataa gattggcatt aggtaagccg gcaagaggaa tagtatccca
     1681 acggagttct cctacttcca aaggagaata attctgagtg gaaaacagag taagattact
     1741 ttcagttgtt ccggtatata actttaaatc tgtagttata gaagtagcat cgccaatatt
     1801 ggttcctaaa actttcagga ctagagaatt tccctcttgt tccagtttaa tatctttcag
     1861 aaacagatca ggaccacgaa caatgtaatt ttttaggaaa ctctctgtaa tacctgtttc
     1921 ggtttccata acatacttga aaaatatctc ttttccagta ggatatttac caagatagtg
     1981 ggtagttaca tacaaagtgc tatcttgttc agacctctgc attggaagtt gaagccagtt
     2041 aatttgagta gaaacactat ctgtcctgac cttgcagata atattgttaa ccggtaaagg
     2101 actaaagacc ttggcagtaa acaagacaga atccgaccag gcaggttctg gaggattaag
     2161 atgatgatgc ataatattag gtctaccaac actaaattgg gaaagcccta tatattcatc
     2221 agtattggaa taaccggcaa ccataatttt acgggtgtag ttattggctg caggatttgt
     2281 attaacataa gtagctgtcc aattcccgtt cggaaagaca ggtaaatcat aagggacatt
     2341 tacaatcttt ccactctctt tcataataaa cagacggcct gcattaacac cggtggggaa
     2401 agtagcatta acgcgtaatg tatcacccgg gttcagcaca tagttatctg caaaaacagg
     2461 tatatctttt atgggcttct tcaatttgat taaaggatca cctaaatagg caaaaccgtt
     2521 gataagggca taaatagctg caggtgaata tatagtagta taagcgcggg ctaaagcaaa
     2581 aatattcgct agaccgatat tggcaaaatt atgtttataa agagcatcac agtatgccat
     2641 accccaggtt tcatcatggt ctaaataacc cagaccagta aaacctgcag tgcctatagc
     2701 tcctttattg ggctgaagga ctaatgattc actgatagaa gccattccgt ttgtatcaaa
     2761 agcggaagca taacaggcaa gagataaaac cacaggatag gtctgattgt tgagcgttgc
     2821 tacatcatta aaattgaaga ggttataatc cgcccaaatt ctacccccgc catgacccat
     2881 aaattgaaca tattgagttc cggaattaat agcatctttc agattgaaag ttccgccaaa
     2941 gtaatctgaa ctaaccgttt gcgtggaagt ataaaccctg gtgactctat attcatcagg
     3001 aatagtttta cggattatac gttctgattg ttgagcaaag atatcattat tatcagttat
     3061 tttcccccca gaagtgaaag ttaaatgact gttccaaaga cgagaggttt gtggattgtt
     3121 acgataagca gttgccttag cggcataatc caaaatttgt tgttccttcc agatactaat
     3181 tcttgcaata gatatatcgg ggacaatatc tgttcctaca atacatccat accaggggtc
     3241 acttgccgta gcaccatgtt tataagtcca ggtcttttta accggaatga gattatatat
     3301 tcgggaagga ctgttatccc tggtatcatc aactccttca cccaaaagaa taacactgct
     3361 taattgaggt ggactccaat tgttataagc ataacgaaga aagtccctaa ttggttcagc
     3421 acctgtaatg ccagcattaa attcatcata gatatcctga acatctacaa tttttactgt
     3481 atgcccttcg gattcccaga gggacttcaa ctgcaaagtt ccttctactg aagtaaattg
     3541 ccagggtgca ataacaatta catcggcact attatcagga ttttttaggt cgctgggaaa
     3601 attgaggcgt attgctttgg gagttttttt catattttca gttacggcaa aatatctgac
     3661 tgcttcggaa aaaacactat cttgaatagt aaccgtccaa ggagcatcac cattaatatt
     3721 aaacggctct atctgagcag aattgaagat actggagccg attttataaa ccgaaatatg
     3781 aggattgctg aaaccgctaa cctcaaattg atataaacca ccaggacgat tggagggctt
     3841 tgtaaacttg atataatcct ggtcagtttt atattcgcgc cagtatttaa tttcagccca
     3901 atccagcatt acctgttctt tatccgtcat tacagtatta ccggaaaggg caatatagaa
     3961 gttattggtt ccatgcctta aaaaggtgtt ggaaatatga ttttgatttt caaatatctt
     4021 ttccgtttgg ccaatccatg tatgcgaatt aaccattgcc tgattcaaac gaatagatgc
     4081 ttcatgatca tattgtccgc tggttagtgt ttcggaataa gttaacccca ttaaacaaat
     4141 tctggcagaa gcaaagcggg ttgtagtatc tttgggatat tgcagttcca cgggaacaat
     4201 ttccagattg ggagcattga tttttttcca gaaccaaaca tcttcgcgat agaaatttgg
     4261 ggtactggaa ctccagcccc taccaagttt atcgcttacc agttgttctt caaagtgaac
     4321 tgtatcttca taagcatcag gaacaatata gttcagaaga ttggattcaa tcaaaccccc
     4381 attttccaca gccattcttg ctccatagtg atcaatcagt ttcagggtat atacattttc
     4441 gcttgtataa tcatccatat agcaagtgtc acctttatgt ttatcaccat agaactcaaa
     4501 atagtcattt tcattgaaga caccgtcatt ttcgcctaca aagtgaatcg gcacctgtcc
     4561 atattcatca gttaattcca gataacgcgg gtctacattt gcaggtgtcc aattcatcac
     4621 tatctgcaaa gaatcagcca tcaaatttat ataatccatc aagtaatgat aactgacctt
     4681 atagattccc tctttatcta caataatctg gatttcgtta atgctggaaa tattattttt
     4741 aggtgcagaa taatttatgt cccgggtttt ttccagacgc caggttttag aactattttc
     4801 attcaagaaa aaaggcgggt ctgcattatc aagaggattt ggattcagtt gccagttttt
     4861 agttgcgttc ttcgttccgc ttatatagac agttatagat atttgggagt gcacaatcag
     4921 ctctttgctt tgtgctctat attgaaaagg ataaatcagc aaaggtatga acttgcggtt
     4981 acctataaaa gcaggttcac ctttctgcgc cagagaaaca ggatagagtt cacgattggc
     5041 ataagctttg tagtccggtt tattattata gtttacttcc tccccttcaa ttaccagagt
     5101 tgacgaaggg atgatattta tattcgtcag cgttttggat tcgctgcttt taatactgaa
     5161 ggtgtaatcg ccatctacag gaactgctat agccgtagaa aacattatca attgaggaaa
     5221 accctcatta ctgagataag aaccatcgct acaaacaatt ctttcccagt tctgtccgtt
     5281 tattgtaact ttggttaatt catattcagg aagctcaaaa ttaatagcaa gtatgtcctg
     5341 actttcagaa acaagagtta ttggctctgc cataattaag gcaggcaggc aaaaactaag
     5401 gcagaagaat aaaatttttc tgcaggaaag ggaacgaaac aggttactca cccggttaat
     5461 ataatacatt ggcataatat tagctatctc ccttgttata aaaggtattt ttacgtaatc
     5521 caatatcttg gcggaaaatt ctgttctcaa aggttatctg ttgaactttg ctgtatgcct
     5581 cttccctggc ttcatttaaa tcttttccca ttccaactgc acataaaaca cgtccaccag
     5641 aggtcacaag tccattttca cctttttcca ctccggagaa ataaattccg gaaaggtctg
     5701 caggtaaaga aagtggaaaa cctttattgt aagtaaaagg ataaccctcg gaagccaaaa
     5761 caacacctaa tgcggtttta tctttgaatt ttagttccac caaatttatc tcaccctgta
     5821 aaatggcttg gcagatttct acaaaatccg tctccagtaa aggtaaaaca acctgtgctt
     5881 cagggtctcc taaccggcaa ttgaattcca taactttcgg tccttcctta gtaatcatca
     5941 gtccacagta gagaattccc ttataaagat gacccaattc gcgcattgcc ataagaacgg
     6001 gattaattat ttcctcttca atctgtttcc ggtatttttc cgcttcataa actggagcat
     6061 aagcacccat tccacctgtg ttgggacctt tgtcaccggc ataaagttgt ttatggtcct
     6121 gagcaaaaat tgtagtttta aactcccagc catctgtaat ggcaaataaa gagacctccc
     6181 aaccctgtaa aaattcttct acaacaactc ctttttcgct aaaatgaatt ttttttaatg
     6241 cttcttgcgc ctcagccgga tttttaacta tgaaaactcc ttttcctcca gctaagccat
     6301 catttttaat aaccgctgga aagccaaagt agtcaatatg ttctaacgct tgaggaatgg
     6361 ttttagctat tttgaatgcg gcagttggaa tacctttttc ctgcatcaaa tttttggcaa
     6421 aaatcttgct gctttccaat tgggcggcaa atttaacagg agctataaca gctactccct
     6481 tctcatttaa ataatcagct aaaccatctg ctatgggttt ttcgggtccg acaaagacaa
     6541 gatcaattag attctgaaca caaaaattgt atatctcttc ttgtgtttgc agatttgcac
     6601 attcatattg aagcgcaatt cctgcattcc cgggagccac aataaccctt tttatctgtt
     6661 tgcttttagc aaaacactct gcaatagaat gttctctgcc tccgctgccg attactaaga
     6721 tattcaatta tcattcctgc cttgtaagtg ctgaattaaa gaaaggatag caaaatcagc
     6781 tgctttaatc cggataattt ccctatcccc ggtaaagaat tgttttttag accaatattt
     6841 ctcagcggct ataaagccaa aacacaccgt tcctacaggt ttttctgcag ttccaccatc
     6901 aggacctgca atcccagtta tagaaattga gcaggtagat tttgtcaatg attttattcc
     6961 ttcagccatt tgcaaagcac aggtttcact aaccgctcct tctgtctcta aaactgtatc
     7021 ggatacattc agcagatttt ttttcacggc attggaatat gctatcacac cacccagaaa
     7081 ggagttagat gagccaggaa tatctgttat cagtttttgc accaagccac cggtacagga
     7141 ttccgcaacg cttaaagtgt agatattttt ccacaaaagc gctaataaaa tttctgcggg
     7201 actttcttca tcatagcccc aaacaaaagg tttaataacc ggcagtgctt ttttaacagc
     7261 ttcttcaact ttattagcat cggttccata aaagcgcaag tcaactcttc caatttgagg
     7321 aagccaagct aaagaaacac cttccggcaa gttagaaaga gtaaataact cagccagtcg
     7381 ggattcagct atgccgtaag ttcttaatgt ttttatagat agtggcttgg catcaggaca
     7441 attttgggta ataatatctt tgatatgtgt ctcaaaaata tgtttcatct cggaaggaac
     7501 accctgtaag gcgaagaaca aaaagtcccc ttcccgataa aacaatccgg gagcagttcc
     7561 caggtcgttt cttaaaggaa taaaatcatc gggaaccatt gcctgagagc gattggtttc
     7621 aggtaatggt atatttctga aagcaaacat tttttggata tgttcccaaa tattatcgtc
     7681 aaagtgttgc tttttaccaa agaactcaga aattgcggat ttggttaaat catcttctgt
     7741 aggacccaaa ccaccggtag ttattactat cttatatttt ccccaggtat cttgcagtgc
     7801 ctgttttata gcgtttggtt catcttttac ggtaagggca tattctacag aaattccttg
     7861 tttggcaagt tccgaaccta aatatgccag attagtattg atggttttac ccatcagaat
     7921 ttcattaccg atagtaataa tagctgcatt cattttagta tgccttggca aaaattactc
     7981 tgcgtgtaga aggtttacca cagcagatac aatttcccgt ttcttcttta gtattaaaag
     8041 ggatacagcg aatagtcact ttcaggtctt ctttaatttt ttcttcgcag gatgattttc
     8101 cacaccagtg agaactggca aaaccaccat gaatttccgg ttgttcaatg tttttaggcg
     8161 taaagaacct atagaattcc tctttatcgt caatttccat tgtccattta tctttaaaag
     8221 ccaaagctcg ttgataatag ccctgttgca tttctgccag agtttgaaga acataatttg
     8281 tgaggtcgga aatctttact gactgcttat cttgtggttc tttatcgcgt ctggcaagca
     8341 taacagaatc ctgaataata tcccgaggac ctatttccag gcgcaaagga atgccttttt
     8401 ttatccaata ccaatttctt tcactgctgg tcatatctct tgtatccaat tcagtataaa
     8461 ggggaacatc atctaaatat aaatccttta gctggttttg aaccttttcc gcaaaagcca
     8521 tcacctgttt ttgttcttgc gcatctctat aaatagggat aatagcaatg tgggaaggag
     8581 caattttcgg aggcaaaaca agtccgttat catcgctgtg tgccataatt atagctccta
     8641 ttaaacgcgt agaaacaccc caggaagtag tccaggcata ttctatttct cctttgcggt
     8701 tttgaaattt gatttcggat gccttggcaa aattctgtcc caaaaaatga gaagttccag
     8761 attgcagtgc ttttctatcc tgcatcattg cttcaataca atatgtagtt acagctccgg
     8821 gaaatttttc actttcggtt ttttcgcctt gaataacagg cagggcaaga tagtcacgcg
     8881 caaaggtagc atatacattg agcattttta aggtctcttc ttttgcctct ttttctgttt
     8941 catgcactgt atgcccttct tgccatagaa attccgtggt gcgtaaaaaa agacgcgtgc
     9001 gcatttccca acggacaata ttagcccatt gattgatcag gagaggcaaa tcccgatagg
     9061 aatttaccca tttagcaaaa gatgcgccga taattgtctc actggtagga cgcacaatat
     9121 aaggttcggt aagttcaccg gctaattgta aaccgccttt tccatctgct tctaatttac
     9181 tgtgtgttac aatagcgcat tctttggcaa agccctctat atgttcagct tctttttcaa
     9241 aaaaactttt aggaatgaaa ataggaaaat aggcattacg atgtcccgtt tcccgaaaca
     9301 ttttatccag tgcacgctga atattttccc aaattgcata gccccagggt tttattacca
     9361 tacaaccgcg aacttcacta ttttcagcca tatcagctgc ttttataacc tgttgatacc
     9421 attcggcata attttcttcc cgggtagggg ttattgccgt tttgttattt ttactcatca
     9481 cttttctcca aaattagtgt ctgaatagtt cggctgctta ttatgcaaaa ccaaaaggaa
     9541 tatattagct atattaccat tagaaagcca attcctgaat agtactattg ttgtcaatgt
     9601 aaaaaggaaa gacaggcata tactgctttt tcccaaataa tatcaatttt tgaatgttca
     9661 gccatttgtc atttctgtga aagcacaaa
//
LOCUS       DAOPFL010000064         9648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_8768_length_9648_cov_107.264776, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000064 DAOPFL010000000
VERSION     DAOPFL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8768_length_9648_cov_107.264776"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            799..874
                     /locus_tag="PLG69_05425"
     tRNA            799..874
                     /locus_tag="PLG69_05425"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:832..834,aa:Ala,seq:ggc)
     gene            959..2851
                     /locus_tag="PLG69_05430"
     CDS             959..2851
                     /locus_tag="PLG69_05430"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPI42986.1"
                     /translation="MKTDIQELINTIKTQANEARKAILTMTTLAGSGHPGGSMSSIDL
                     LLCVYNTIKHNPAQPQMPDRDRVIISNGHISPAVFSALGLLGYFDLDEAIAQYRLTGS
                     IFEGHTERRVPGVEWSSGNLGQGLSAGTGFALASRINKIPYNVYVFMGDGEQQKGQIS
                     EARRFAVKYCLNNIIAIIDYNQLQISGSIHNVMPQNIRKNWESDGWKVMEVDGHNIPL
                     ILTALDNARAFQQPTMILAHTVMGKGVSFMENNAQYHGSALSEAQLENALKELGTENE
                     LKKYRKLRSEFKPEHNPQISFAFEPDFTIGKPVRYETDNDCRSAWGKTIADLAKVNAN
                     AKTPIVVLDCDLATSVKTEEFAQVSPQNFFQCGIMEQHTCVMGGALSLCGIQTFWSDF
                     GVFGIAEVYNNQRLNDINQTNLKTVVTHTGIDVGADGKTHQCIDYISLTRNLFGFKLI
                     FPADANQTDRIIRWLINKPGNYLVTMGRSKLPVLKKLDGTLQYDVDYNFIYGKADILR
                     DGNDGSMWVCGTPIAKAVKAVDTLREEGIFLELLYVSAPLHLDLAQVKFSAGKGIIFS
                     VEDHSVNGGLGTSIADALAVNFLGAKLVKIGVSQYPLSGEANELYHWAGLDTASLIAT
                     VKKYLA"
     gene            complement(3275..3571)
                     /locus_tag="PLG69_05435"
     CDS             complement(3275..3571)
                     /locus_tag="PLG69_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42987.1"
                     /translation="MKDKIIAKQDLIPIIEDIDAIGFYGMLPKHIRGEYDASLFEVDD
                     AIDCRDTFIQQAYENNLLSDEQIREVKKIDDYILRHRVPEELGEFKEWLKGHQI"
     gene            complement(3702..4061)
                     /locus_tag="PLG69_05440"
     CDS             complement(3702..4061)
                     /locus_tag="PLG69_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPI42988.1"
                     /translation="MNKNSNERMQVRYSDAFKLKVVQDIEKGKLTISEARRLYEINGT
                     NTVYRWLRKYGKNDLIQKVVRIEMKDEKSQLKKKDERIRELESVIASLTLHNLCLLVW
                     KEDIDSKKGSCLQVLLI"
     gene            complement(4183..4440)
                     /locus_tag="PLG69_05445"
     CDS             complement(4183..4440)
                     /locus_tag="PLG69_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42989.1"
                     /translation="MKTGKIIDVKCRCGYTLFRYYKAGKGRLIKCIISRLTEDFVGLK
                     GAETFSRPKCPKCGKELGIIKMIHGEPALKLNQGTIETIRL"
     gene            complement(4441..6864)
                     /locus_tag="PLG69_05450"
     CDS             complement(4441..6864)
                     /locus_tag="PLG69_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPI42990.1"
                     /translation="MKVNIEHLLPFVEKPSRYIDNEINAWRKDFPAFQVHFAFAFPDT
                     YELGISHLGIKILYSLINELDYAMADRFYLPWIDLIDLMREEELELFGWESRTQVKDF
                     DILGITLQSELNYTNVLELIKLSGIDIHSRQRQETDPIILAGGPCTVNPLPLAPFIDV
                     FFIGEAEEGIIQIAEILKNEKKRAERIKQISKLPSCYVPALHNGIDLIHCRKYTAFHT
                     SKSQHSPQLLSWQLATHNRYVSEIMRGCSRGCRFCFAGYFYRPVREREPNAIIENILK
                     EIKASGWDEVGLLSLSSSDYSCIQNLLLQLLKFIETQKTHIALPSLRVDTLDKEIGQA
                     LKSLGREGLTIAPEAGSEHLRQVINKNLSEQQILAGIETALKFGWQKIKLYFMLGLPS
                     ETEDDIEAIIKLIHKINEQGKRRLQINVTLSPFVPKPFTPFQWCAMEDAESLLKKAIK
                     IKSAFAKAKNIKIHYHTIENSLLEALLSRGDTKIAEVIEVAFNKGARFDGWNECFNFS
                     WWQEAIAECNINLNIYLGSRNPQDKLPWDFISLGIDKNFLLSEWEKAKAGIQTPDCRE
                     LCSSCGICNEEVQTKTAKGSLLETDNLQQNKKIPLYQASQYKYRVYYQKTGLLRFISH
                     LDWMRMLFRRISVLELKTVFTQGFNPHPKVSLCPPLAVGIEGLAEYFDLSFYQPYPAE
                     LILQEFNKTKIPDFTVTAVELIKTKITPPRTELLSTFFPDSLYLHIRDKIKFFKLSKI
                     FTYTKGTPPKVKNYDLKEIIVSINLIGNELQLEKLLESPSVYEILPAVLTLEKTMLYQ
                     QKIYRNGFR"
     gene            complement(6854..7573)
                     /locus_tag="PLG69_05455"
     CDS             complement(6854..7573)
                     /locus_tag="PLG69_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPI42991.1"
                     /translation="MNIGGLQKFSLLDYPGQLSAIVFTQGCNFRCPYCHNPELVDPQR
                     YSPLLETEKVLRFLYRRHKKLSAVVVTGGEPTLQEDLIPFLKLIKALHYKIKLDTNGS
                     RPEVLREIVNQGLVDYFAMDIKAPLKLYKVITRADLEVGTIEKSMELIRRSGVDYEFR
                     TTYFDLLLSTDDLASMQDLLKPGDKFIIQQCHYGKTLDEIKREDIVTGSFSFNENPAC
                     LSLIHWGTEHSIKISLRSYES"
     gene            complement(7583..>9648)
                     /locus_tag="PLG69_05460"
     CDS             complement(7583..>9648)
                     /locus_tag="PLG69_05460"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPI42992.1"
                     /translation="KAIESAGLATGEFGKDIAGVLTLRVLNLAQQVIQDEIPEVEQIQ
                     DIVEEVLLASPYKKTAKAYIIYRDQHSRIRELVSKAGVDLIDQYLDKLDWQVNENSNM
                     AYSLQGLNNYIASEVSKTYWLNKIYPPEIREAHLSGDLHIHDLGQLSVYCVGWDLMDL
                     LLTGFCGAEGKVESTPAKHFRSALGQIVNFFYTLQGEAAGAQAFSSFDTLLAPFIYYD
                     RLSYKEVKQALQEFVFNINVPTRVGFQTPFTNITLDLKPHQIYKDQAVIIGGKPRDKN
                     YGDFQAEMDILNKAFLEVMIEGDAKGRVFTFPIPTYNITKDFEWDNPNLDYLWEVTAK
                     YGIPYFSNFVNSDMDPKDARSMCCRLRLDTRKLETRGGGLFGANPLTGSIGVVTINLP
                     RIGYLAKTEEEFFQLLEERLLLAKKSLEIKRKVLENFTTGGLYPYTKFYLKSIYERFQ
                     QFWKNHFSTIGIIGMNEAMLNFMGENLGTKKANAFALKIMDYLRKRLLEFQEETQNNY
                     NLEATPAEGTSYRLALLDRKHFPDIICANCDADSPFYTNSSQLPVNFSDDIFEVLDLQ
                     DELQTKYTGGTVLHIFGGERVEYGQSIKALVKTICEGYRLPYFTFSPTFSICPQHGYL
                     DGEQPICPLCQKKTEVFSRIVGYLRPVSQWNEGKQAEFKMRTNFDLQKSKKKDSSAIA
                     KSHFA"
BASE COUNT         2898 a         1891 c         1788 g         3071 t
ORIGIN      
        1 tagtgcaatt tataatctct ggtagggggg tacttatacc ctttactagc catttcgttg
       61 tgtagacgga ggacgctgtt cctccgcttt atttttggga aaacactatt ctcattgttt
      121 aaccttctgc caagctattg cattaccaaa atgtttattg gaaaagaact gccggattgg
      181 atatggaaag cgaggactcc gtatctccgt tattttgatt ctttagtgaa aggttaggtt
      241 taacgataat gctgagttgg atgtaagcgg aaataattcg gtttatccgt tattttgctc
      301 ccttaccgca aaaattagtc ataagggaaa tatggaattg gagttatagt gtgcggaaga
      361 tttcactttc tcaacctcat attagttgta aggtgaagtc gtcaatcaat agttaatcat
      421 acattaatca agcgttaatt attaacgctt gattaacact tgattaagga cagattaacg
      481 attttggcta atggctaata ggaaaattag tatatattat actgattttt acaaaggtag
      541 caattgcctg aagtataaat tccatacgca atctttagtt tccagagtaa agaaaaccct
      601 ttccaccctc tattttcctg ttagaagatg aaattaaatc aatactaccc caatgcagtt
      661 aatatagaga aatattagtt tttcaattga caatccgaca gtaagttaca gaataattaa
      721 gtaaaaatgc tgtagtttct gcttgacaga attttgccct ttgcaggcaa tgcactcata
      781 taaagtgatt tttctgttgg ggcattagct cagctgggag agcgcatgac tggcagtcat
      841 gaggtcagcg gttcgatccc gctatgctcc accatttttt attccccttc atcaaaaaat
      901 gaattggtga aggggataaa cttttttgca agagaaaaat agaagataag gaaaacaaat
      961 gaaaaccgat attcaggaat tgattaatac tattaaaaca caggcaaatg aagccagaaa
     1021 agcaatttta acaatgacaa ctcttgccgg ttcgggtcat cctggcggtt caatgtcctc
     1081 aattgacctt cttttgtgcg tgtataatac aataaaacat aatcctgctc aaccccaaat
     1141 gccagataga gatagagtta taatcagcaa tggacatatt tctcccgctg ttttcagtgc
     1201 tcttggactt ctgggctatt ttgatctgga cgaagcaatc gctcaatatc ggcttacggg
     1261 aagtattttt gaaggtcata cggaacgcag agttccgggg gtggaatgga gtagtgggaa
     1321 tttaggtcag ggtttatctg ccggaactgg ctttgcactt gcttcccgga taaacaaaat
     1381 tccctacaat gtttatgttt ttatgggtga cggagaacag caaaaaggtc agattagcga
     1441 ggcaagacgc tttgcagtaa aatattgcct aaataatatt atcgctatta ttgactataa
     1501 tcaattgcag ataagcggtt ccatccataa tgttatgcct caaaatatcc gaaaaaactg
     1561 ggaatcagat ggctggaaag tgatggaagt tgatggtcac aatattcctc ttattcttac
     1621 tgcccttgat aatgcacgtg cttttcagca accgacaatg attctggcac atacggtaat
     1681 gggtaaaggt gtgtcattta tggaaaataa tgcccaatat cacggttctg ctttgtccga
     1741 agctcaattg gaaaacgcct tgaaggaatt gggaacagag aatgagctga aaaaataccg
     1801 taaactgcgc tcggaattta agccggaaca taacccgcaa atcagttttg cttttgagcc
     1861 cgattttaca ataggcaagc ccgttcggta tgaaacagat aacgattgca gaagcgcctg
     1921 gggaaaaaca attgccgatt tagccaaagt aaatgctaat gccaaaacgc caattgtggt
     1981 tttggattgc gatttggcta caagcgtaaa gacggaagaa tttgcccagg taagtcccca
     2041 aaactttttc cagtgcggga ttatggaaca gcatacttgc gtgatgggag gagctctttc
     2101 tctttgtgga attcagacct tctggagtga ttttggtgtt ttcggtattg cagaggtcta
     2161 taataatcaa cggcttaacg atatcaatca aaccaatctg aaaacagttg ttacacatac
     2221 aggcatagat gtaggtgcag acggaaagac ccatcagtgt attgactata tttctttaac
     2281 cagaaacctc tttggcttta aacttatctt tcctgcagac gccaatcaaa ctgatagaat
     2341 tatccgctgg ttgattaata aaccaggaaa ttatctggta acaatgggac gctccaaact
     2401 acccgtgctg aaaaaattag acggaacttt gcaatatgat gtggattata actttattta
     2461 cggtaaagca gatattctta gagacggaaa tgacggcagt atgtgggttt gcggaactcc
     2521 tattgccaaa gcggtaaagg cagttgatac cttacgcgaa gaaggtattt ttcttgaatt
     2581 gctttatgtt tccgcaccct tgcatcttga cctggctcaa gtaaagtttt ctgctggaaa
     2641 aggtataatt ttcagtgtgg aagatcattc tgtaaatggt ggcttgggaa cctctattgc
     2701 tgatgctctt gccgtcaatt tcctgggtgc aaaattagtt aaaattgggg tttcacaata
     2761 cccgttgtcc ggagaagcaa atgaactcta ccattgggct ggcttggata ctgcttcact
     2821 tattgctacg gtaaaaaaat atctggcata aaaatgaaga atgaaacttt ctaacataga
     2881 gataaaaaaa gaaagaggat tattttacaa agaggaaaag agctaaagag aattaattga
     2941 caattggaaa tggagaattg agaatggaac ttaggattta ttagcggtat aataccattt
     3001 ttggaatgaa aagacctttg tcattcctgt gaaagcagga atccaaagta gtttggacaa
     3061 aataactgta ttttcaagat agaaacattg taagtccggg ggtcatttgc gctttgatat
     3121 ctttgatggc gcttatgact tataacgaca tagaattatc tctcacagat aacacagatt
     3181 tcacagattt ataattttta atttttttaa ttctccattc ttcatttttc tctcacagat
     3241 ttcacggata acacagatat atattagaaa ggatttatat ctggtgtcct ttcagccatt
     3301 ccttaaattc ccccaattcc tccggaactc tatgtctcaa aatataatca tctatcttct
     3361 tcacttccct tatctgctca tcacttaata aattattctc gtatgcctgt tggataaatg
     3421 tatccctgca atctatggca tcatccacct caaataatga agcatcgtat tcgcctctaa
     3481 tatgtttcgg caacatccca taaaaaccga ttgcatcaat atcttctata atcgggataa
     3541 ggtcttgttt ggcaattatt ttatctttca ttctctttat ttctcctttt tttattcttg
     3601 tcccttccta cataatcaac actttaatcg tcttttctcc cttcaattct ttctctatct
     3661 ggcttgcttc ttttttatga taagttagaa ccttttgtca attaaatcaa taatacctgt
     3721 aaacagcttc cttttttgct gtcaatatct tctttccaaa ccagcaagca taagttatgt
     3781 agagttagag atgcaataac ggattccaat tctctgatcc gttcatcttt ttttttgagt
     3841 tggctttttt cgtctttcat ttcgatcctc acaacttttt gaattaaatc gttttttccg
     3901 tacttccgca gccatcgata gaccgtgtta gtaccattaa tttcataaag acgacgagct
     3961 tcacttattg tcaactttcc tttttcgata tcctgtacaa ccttcagttt gaaggcatcg
     4021 ctgtagcgga cttgcatcct ttcattagaa tttttgttca tttttttctc cttaatttta
     4081 gtccgctatt ttaaggacac ctcaacacct catacatttt acattctaca ttttacattc
     4141 ttcattaaac tttgctctct tttctctttg tgaaataaga agttacagtc ggattgtctc
     4201 aatcgttccc tggttaagtt tcagagctgg ctctccgtga atcatcttta tgattcccag
     4261 ttccttacca cattttggac acttgggtcg agaaaaagtt tcagcacctt tcagtcctac
     4321 aaaatcctca gttaatctac ttattatgca tttgatcagg cgtccctttc ctgctttgta
     4381 ataacgaaaa agggtgtatc cacagcggca tttaacatca attattttgc ctgttttcat
     4441 ttagcgaaag ccgttacggt aaattttttg ctgatagagc attgtttttt ccagggttaa
     4501 aacagcaggc agaatttcat atacagaagg gctttccagc aatttttcca gttgtagttc
     4561 gttaccgatt aggtttatgg aaacgataat ctctttcaga tcatagtttt ttactttagg
     4621 aggagtgcct tttgtgtaag taaatatctt acttagctta aagaatttga ttttatctct
     4681 tatatgcaaa tataaggagt cagggaaaaa agtgctgagc aattcagttc ttggcggagt
     4741 aatttttgtt tttatgagct caacggcagt tacagtaaaa tcaggaattt tggttttgtt
     4801 aaattcctgc aaaattagtt ccgctggata gggttgataa aaagagagat caaaatattc
     4861 tgccaaaccc tcaattccaa ctgctaaagg cggacataaa gaaaccttgg gatggggatt
     4921 aaacccttgt gtaaagacag ttttcagttc taaaacggat attctgcgga aaagcatacg
     4981 catccaatcc aaatgagaaa taaagcgtaa aagtcccgtt ttttgataat aaacccgata
     5041 tttatattga gatgcttgat aaagcgggat tttcttattc tgctgcaagt tatctgtttc
     5101 taacaaacta ccttttgctg ttttagtttg aacttcctca ttgcaaattc cgcaggaaga
     5161 acaaagctct ctgcaatcgg gtgtttgaat tcccgctttt gctttttccc attcggaaag
     5221 cagaaaattc ttatctatac ccagggaaat aaaatcccag ggcaatttgt cttgcggatt
     5281 gcgtgaaccc aaataaatat ttaagttaat gttacattca gctattgcct cctgccacca
     5341 ggaaaaatta aaacattcat tccaaccatc aaaacgggca cccttgttaa aagcgacttc
     5401 aataacttcc gctatttttg tgtcaccccg gctaagtaat gcttccagaa gggaattttc
     5461 aattgtatga tagtggattt tgatattttt agctttagca aaagcggatt tgattttgat
     5521 tgcctttttc aataacgatt ctgcatcttc catagcacac cactgaaaag gagtaaacgg
     5581 tttggggaca aaaggggaaa gagttacatt tatttgtaga cgcctttttc cctgttcatt
     5641 gattttatgg ataagcttaa taattgcttc aatatcgtct tccgtttcac ttggcaaacc
     5701 aagcataaaa tagagcttaa tcttttgcca gccaaatttt aatgcggttt caattccagc
     5761 caggatttgt tgttcgctca agtttttatt tatcacctgg cgtaaatgtt cagagccagc
     5821 ttcaggagct atagttaagc cctctctgcc caaactttta agcgcttgac cgatttcctt
     5881 atccaaagtg tccactctta aagagggaag agcaatatgg gttttttggg tctcaataaa
     5941 tttcagcaac tgtaatagca aattttgaat gcaagaataa tcactgctgg aaagagatag
     6001 caagccaact tcgtcccaac cgcttgcttt tatttctttt aggatatttt ctatgatagc
     6061 attgggttcg cgttcccgca caggacgata aaaataaccg gcaaaacaaa atcggcaacc
     6121 ccgggagcaa cctctcataa tttcagatac atagcgatta tgagtagcta attgccagga
     6181 aagtaattga ggactgtgtt gtgatttaga tgtatgaaaa gcagtatact tacgacaatg
     6241 aataagatca atgccattat gcagggcagg aacataacag gatggtagtt tggaaatttg
     6301 tttaattcgt tcagcacgct tcttttcgtt ttttagaatt tcggcaattt gaatgatgcc
     6361 ttcttccgct tccccgataa aaaacacatc tataaaagga gctaaaggta aaggatttac
     6421 ggtacaagga cctcctgcca gaattattgg gtctgtttct tgccgttgcc tactgtgaat
     6481 atctattccg cttaacttaa tcagttccag cacattagtg taattaagtt cactttgcag
     6541 ggtaattcct aagatgtcaa agtccttaac ctgcgttctg ctttcccaac cgaaaagttc
     6601 caattcctct tccctcatta aatcaatcaa atcaatccag ggaagataaa acctgtctgc
     6661 catagcataa tccaattcat tgatcaggga atagagtatt tttatgccca aatgtgaaat
     6721 tcccagttca taagtatcgg gaaaagcaaa agcgaaatgg acttgaaaag cgggaaaatc
     6781 ctttctccag gcattaattt cattgtctat atatctggat ggtttttcca caaaaggcag
     6841 aaggtgctca atattaactt tcataactgc gcaaagatat ttttatgcta tgttctgtgc
     6901 cccaatggat taacgataga caggcaggat tttcattgaa agagaaagaa ccggttacta
     6961 tatcttctct tttgatttca tccagggtct tgccgtaatg gcattgttga atgataaatt
     7021 tatcgcccgg cttcagtaaa tcttgcatag aagcaaggtc atcagtgctt aaaagcaaat
     7081 caaaataagt agtgcggaat tcataatcaa ctcctgatcg gcgaataagt tccatacttt
     7141 tttctattgt tcctacctcc aaatccgctc tggttataac tttataaagt ttcaacgggg
     7201 cttttatgtc catcgcaaaa taatccacca gtccttgatt aacgatttcc cgtaagacct
     7261 caggacgaga accgttagta tccaatttta ttttgtaatg cagtgctttt attaacttta
     7321 gaaaaggaat aaggtcttct tgcaaggttg gttcacctcc tgtaactact actgcggaaa
     7381 gtttcttatg acgacgataa agaaaacgca gaaccttttc tgtttccagc aaggggctat
     7441 atctttgggg atcaacaagt tcgggattat gacaataagg acagcgaaaa ttgcagccct
     7501 gcgtaaaaac aatggcagat aactgccccg gataatccaa cagcgaaaat ttttgcaacc
     7561 ctccaatatt cattatctgg tcctaagcaa aatgactttt ggcaattgcg gaactatctt
     7621 ttttcttgct cttttgcaga tcaaaattgg tgcgcatctt aaattctgct tgcttgcctt
     7681 cattccattg agaaacggga cgtaaatagc caacaattcg ggaaaaaacc tctgtctttt
     7741 tttgacagag agggcaaatt ggttgttcac catccaaata accatgttga ggacaaatac
     7801 tgaaggttgg agaaaaagta aaatagggca aacggtaacc ctcacagatt gtttttacca
     7861 gggcttttat gctttgtcca tattcaactc tttctccacc gaagatatgt aaaaccgttc
     7921 caccggtata tttggtctga agttcatcct gcaaatccaa aacctcaaag atatcatccg
     7981 aaaagtttac aggcaattga ctgctattgg tataaaaagg tgaatccgca tcacaattgg
     8041 cacaaatgat atcgggaaaa tgttttctat ccaaaagcgc caaacgataa cttgttcctt
     8101 cggcaggagt tgcttccaga ttataattat tttgggtttc ttcctgaaat tccagtaaac
     8161 gcttacgcag gtaatccatt atttttaagg caaaggcatt agctttcttt gttcccaagt
     8221 tttcacccat aaagtttaac atcgcttcgt tcataccgat aattccgata gtggaaaaat
     8281 ggtttttcca gaattgctga aaacgttcat aaatgctttt cagatagaac ttggtgtaag
     8341 gataaagtcc accggtagta aaattctcca gcaccttgcg ttttatttcc agggattttt
     8401 ttgccagcag taaacgctcc tccaaaagct gaaaaaattc ctcttccgtt tttgctagat
     8461 aaccgatacg aggtaaatta atagttacca ctccgatgga accggttaaa ggattagcac
     8521 caaaaagtcc gccacccctg gtttccagtt ttctggtatc caaacgcaaa cgacaacaca
     8581 tactgcgtgc atctttgggg tccatatcgg aatttacgaa attggaaaaa tagggaatac
     8641 cgtatttggc agttacttcc cataaataat ccagattagg attatcccat tcaaaatcct
     8701 tagttatatt ataggtggga atcggaaaag tgaaaacccg acccttagca tcgccttcaa
     8761 tcatcacttc cagaaatgct ttgtttaaaa tgtccatttc tgcctgaaaa tcaccgtaat
     8821 ttttatctct tggcttgccc ccaatgataa ctgcctgatc tttataaatc tgatgcggtt
     8881 tcaggtctaa agtgatattg gtaaaagggg tctgaaaacc aaccctggta ggaacattga
     8941 tgttgaaaac aaattcctgc agtgcctgtt ttacttcttt ataacttaaa cggtcataat
     9001 aaataaaggg tgctaaaaga gtatcaaaac tggaaaatgc ttgtgcgcct gctgcttcac
     9061 cctgcaaagt ataaaagaaa tttacaattt gccctaatgc acttctaaaa tgtttggctg
     9121 gagtactttc cactttgcct tccgctccgc aaaatcccgt taaaagaagg tccatcaaat
     9181 cccaacctac acaataaaca gaaagttgac ccaaatcgtg aatatgcaaa tccccgctta
     9241 aatgtgcttc cctgatttcc ggaggatata ttttattcag ccagtatgtc ttacttactt
     9301 ccgaagcgat gtaattattc aatccctgca gagaatatgc catattggaa ttttcattca
     9361 cctgccaatc caacttgtcc aaatattgat ctatcaaatc cactccagct ttggaaacaa
     9421 gttcccgaat ccgagaatgc tgatcacgat agataatata tgctttggcg gtcttcttat
     9481 aaggtgaggc aagcaaaacc tcttctacaa tatcttgaat ttgttccact tcaggaattt
     9541 catcttgaat aacctgctgt gccaaattca aaacccgtaa ggttaaaact ccggcaatat
     9601 cttttccgaa ttcacctgtg gcaagtcctg cactttcaat tgctttgg
//
LOCUS       DAOPFL010000065         9631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_8786_length_9631_cov_72.170008, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000065 DAOPFL010000000
VERSION     DAOPFL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8786_length_9631_cov_72.170008"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="PLG69_05465"
     CDS             complement(<1..1552)
                     /locus_tag="PLG69_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPI42993.1"
                     /translation="MKRIILFLTTLVLLCTIAKAEELRLDLIFSNDMHGGIDRSEATF
                     INPDFPPLLGGGGSAATLIKHIRSLANDRRDNLLLDAGDIFQGRPVGTITNGKAVVEF
                     MNAIGYDAMTIGNHEYDIPEEKLMETLQLAQFPVLSCNIIDKRTGEIPSYIQPYIVVE
                     RLGLKIGILGFTTTDTEKMSFPENIKNIKFLSEKEAIDKYVKILRNQEKVDIVIVLGH
                     AGLPYDIVPTYLTRYDAQGNPLYSERYAVWGYDAQEIAREVEGIDIFIGGHMHKGIPT
                     PWIDPYTHTMVIQGYAYGSNLGWITLKIDPETKTVTGYEVPAIREGSMVTLFEDEFIP
                     DEEIGEMIAEQVAIAEEGMDEVVGYAGVYLSRTNVDAQSLIGNTIVDAMRAEVGADFA
                     FLNLGGVRADIKAGPVTYRDIFQVMPFDNMIVSFRCNGEFLRRIIETRVEGSRHGLII
                     SGGKVVYSKKRENFDRVTHFEIGGKPWDPNKIYTVATTDFIMQGNAGLTMLIQVPQED
                     IIYHQINLR"
     gene            complement(1560..2987)
                     /locus_tag="PLG69_05470"
     CDS             complement(1560..2987)
                     /locus_tag="PLG69_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI42994.1"
                     /translation="MLSNKSLILICLLIVASALSAWSITDTYFGNPFSTFDARSFAMG
                     GAGLYNSKGVFGIADNPANLTLMRKTLGFSVNTYLNRNEDNRSIPLYNSFDNYIDDSV
                     YSSNINTFDNYALSAFFAHRFGERGLGLGFYYKPVSSFDADYREEVRNNRNTDNDVYP
                     EKIALNKIESEGSLNKTAFVTSFAYSLSDYVDLNLGFEYALLNGDVNREKTIRWTDWA
                     ISQVGEYHLPELTEIDDYELSGDQFKIGASALLSKRFGIAATLTPGTTLDKKGSYYYK
                     RDAYRNTAVDSINTAIKEDYKLPTQIRFGFVYTPRNVVRTEFNMDIEYVAYSEINKRY
                     DDVMNFYAGVEHQVVNFLPLRLGFQAVNNWYFTTEEGTDENNNPITLYHTTKILTPMI
                     TGGSSIKLMDNLKIDLGFGYTWREYEAVDMFGDAYYNDKQYTGSSSYVLWPNSHITLK
                     NRGWENPDKIRENNISLNAGLNFTW"
     gene            complement(3000..5762)
                     /locus_tag="PLG69_05475"
     CDS             complement(3000..5762)
                     /locus_tag="PLG69_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPI42995.1"
                     /translation="MKKFLIMLFVLAGLSVLTAKVDLNTASLAELMQLPITEKQAKDI
                     YEYREFVKIFDDIYELRKIPSIDQETLDRLKPLVVVSIYVETDEAAVRREEIRDLLER
                     VDSNEGASEGMADVWEDYLMTPQNVNKMLFDDLMSFPNVSGIDAVAVLKRIAQGDTIA
                     DTRDLRNSTGLSYYGYSNLRSYVYYKEPPVKNRLMCDASLLYYTRYFEEGQYDMLHES
                     FLRNDYNATNVVIPHLKTNSYWGYFGLDKLDPDIMLKLRMRYGNNYKLGIMHYKAKGE
                     EDLLNIKAEDILADSKWYVGYENSELPGFYNSRLKVYAGNYRATFGEGLVMENTDYYS
                     ARKTGYGFSKRILGITPDLSRTQENSLKGGAVEYTTPLFGVTAFVSVDNKDALTYIDR
                     DGYAVMRDEYGNDIYTDRDTTSDYFGRQYYINNNGEHIYNYADSTAVQGDKTKVFALI
                     NPTLRYDDDTMREAEAYFNNEISAGVPYAMNYINLAARKDVLQEKLWGTHLQINPFLG
                     TKLGFTTYTAIYDDAHFVVPTYNNLLPTVLRDSYYYSKLVKNMNAEISNLYSTQTNKY
                     TRDYRRVIGFDGQTVIGNTSIQGEYAELTVNGDDWKLGDDPKAYLVSAHTQFENLYFI
                     TLFRNYDVDFDNPYSNSFSEHERFEDTILEKNVYALTNPTIADLYLNSNQSQPERGIY
                     FETRYKFNRYYTINRSYLDIWERLTDGRRSVRFQSELEFRPIYQLAMRLRYKNQVNRY
                     DDDAERGVSKTNEYTLAFRTFLSNRDFLELEYRYNTVWGPPYTSLTYPAAEGENSMAA
                     AQTLMIGDYVAVNYTHYFNKSLKMQGSFLYWFGHGISHWDWEDMEIDFMGEKGSKAWI
                     AFTSRVSQNMYLNLKFKNKTYQDKEIRIRNYNLSDDPTLLDDPVYFQRVEHSENTIRL
                     SIDYRY"
     gene            6313..9336
                     /locus_tag="PLG69_05480"
     CDS             6313..9336
                     /locus_tag="PLG69_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPI42996.1"
                     /translation="MQKKFFVFLVALVWSIALVGASVTLDSLEISVQGGGYPRQGSTI
                     VITMYYRVADIPEGESIYVYGIATGAVDQAQIKWYPSSNGTGTASNQNVSVNQVANTI
                     PYDEDGNGYQSSHTATFTLPAPPSSSYKSFKINGKVYAATSDDEFTYFYSGIKLSKNY
                     LTINNPPVASSVVIVDPTLARVGQTLTGDYEISDPDGDEIDTPIFRWLSSTIETGTYT
                     AISGATGNTYTVQSADANKYIKFEVTPIAKTGELQGAPKLSDATPKVTGAPIVEIDPS
                     TLNIYEASANNGSLGDAYVKITCTNTSFKSSITNSAITISKDLDGLSKGSVTWISANE
                     IRLSFSGAAVNHEYAAKIDTIRITINKDQLEGVENNLTTQNGFNINFTNNPPTNLALD
                     SVGNNYVKITWDKPAGLLTSGSGLDYFNILRNETNIGSLYLDKGKGTYYYTDNTVTNG
                     TQYRYKVQAVYTAGTNETSTELRGTPLAITAYSFSSPPATGTIDHTNKTIKVVVPNGT
                     DRNALVANFTAPGAIVKVGTATQTSGSTSNNFTNPVTYTLTTNNDASTCSYVVTVNEK
                     LATPSPSVVGSNITTSSIKIHWDTITGADRYGVDVSENNSFLPGSFQHKEVSANFCIM
                     NGLKAGTKYYYRVQAIAEDQYFNSDYSSVYDTTTDALIPGAGSTEIDSSYETTINVGE
                     YVYGQETVTPTVKVHPAEFSPSENDIITVTMSYGTTPEGLQYNLAFENPTIGNGTFIL
                     SYSGLSYDPTDVGYRLNGGALHSVGGDGINTTDKIVIFTMSGLGKGSKAAYELQIVLN
                     DESGQTLPVVLTSFTATQMVDGKVRVDWVTQSETNLSGFRVLRNTVAEISTAMAVSPL
                     IEATNTSATVVYSFMDSEVPGNGIYYYWLQIEDLDGGISYSAVYTVQVTTGGVTNPII
                     PKITALLNPFPNPFNPDVTIPFDLAVDGNVTLKIYNLKGQLIKNLLNENCKANSYRIL
                     WDGKDNEGHTVSTGTYIIRMNAPNYQASHKISLIK"
BASE COUNT         3060 a         1859 c         1679 g         3033 t
ORIGIN      
        1 cccgtaaatt gatttggtga tagataatat cttcttgagg cacttgaatc agcattgtga
       61 gccctgcatt accttgcata ataaaatccg tagtagcaac ggtataaatt ttattggggt
      121 cccagggttt tcctccaatt tcaaaatgag ttacgcggtc aaaattttcc cgctttttgg
      181 aatagacaac cttgcctcca gagataatta agccatgtct tgaaccctca actcgggttt
      241 caattatccg acgcaaaaat tcaccgttac aacgaaaaga aactatcata ttatcaaaag
      301 gcatcacttg aaaaatatct ctataggtaa caggacctgc tttaatatcg gcacgcactc
      361 ctcccaaatt cagaaaggca aaatcggcac caacttcagc acgcatagca tctacaatgg
      421 tatttccaat taaggattga gcatctacat tagttcggga aagataaaca cctgcataac
      481 ccacaacttc gtccattcct tcttctgcta tggcaacctg ttcagcaatc atctctccaa
      541 tttcttcatc gggaataaat tcatcctcaa aaagggtaac catactgcct tcacgaatgg
      601 caggaacttc ataaccggtt acagtttttg tttcgggatc tatttttaag gttatccatc
      661 ccagattgga accgtaagca taaccttgaa taaccattgt atgtgtgtaa gggtctatcc
      721 agggggtagg aattccctta tgcatatggc cgccaatgaa aatatctatg ccttccactt
      781 cgcgcgctat ttcttgagca tcatatcccc aaacagcata tctttcactg tataaaggat
      841 ttccttgagc atcataacgc gtaagatagg taggaacaat atcataaggt aaaccagcat
      901 gccccaggac aataacaata tccacctttt cctgattacg gaggattttt acatatttat
      961 ctatcgcttc tttttcggaa aggaatttaa tatttttgat gttttccgga aagctcatct
     1021 tttccgtatc ggttgtagta aaacccaaaa tcccgatttt taaccctaat cgctctacca
     1081 caatataggg ctggatatag cttggaattt cacctgttct tttatctata atattacacg
     1141 ataataccgg aaactgagct aactgcaggg tctccatcaa tttctcttca ggaatatcat
     1201 attcgtgatt gccaatagtc attgcatcat agccaatggc attcataaat tcaaccacag
     1261 ctttcccgtt agttatggtt cctacaggac gaccttgaaa aatatcaccc gcatccaaaa
     1321 gtaaattatc tcttctgtca tttgccaaag aacgaatgtg cttaattaaa gtagcggcgg
     1381 aacctcctcc tccaagaagt ggaggaaaat ccggattgat aaaagttgct tccgaacggt
     1441 ctataccacc atgcatatcg ttagaaaaaa tgaggtctaa acgcagttct tccgctttgg
     1501 caattgtgca aagcagaact aatgtagtaa ggaataatat tatccgtttc atagtttttt
     1561 taccaggtaa aattaagtcc ggcattcagg gaaatattgt tttcccgaat tttatcagga
     1621 ttttcccaac cacgattttt cagggttata tgggaattgg gccataaaac ataacttgaa
     1681 gagccagtat attgtttatc attataataa gcatcgccaa acatatccac cgcttcatat
     1741 tcacgccagg tataaccgaa accgagatca attttcaagt tatccattag tttgatgctg
     1801 ctcccaccgg ttatcatagg tgttaatatt ttagttgtat gatataaagt tataggattg
     1861 ttattttcat ctgtgccttc ttcggtagta aaataccagt tatttactgc ctgaaaacca
     1921 agacgcaaag gtaaaaagtt gactacctga tgttccactc cggcatagaa attcattaca
     1981 tcatcataac gcttattaat ttcgctgtag gcaacatatt ctatatccat attgaattca
     2041 gtgcgcacaa cattacgagg agtgtataca aaaccaaaac gaatttgagt gggaagttta
     2101 taatcctctt tgatagcagt attgatacta tccacggcag tgttacgata agcatctctt
     2161 ttgtaatagt aactgccttt tttatctaaa gtggttccgg gagttaaagt tgcagctata
     2221 ccaaaacgct tactcaaaag agctgaagca cctattttga attgatcgcc acttagttca
     2281 taatcatcaa tctcagttaa ttctggaaga tgatattctc ctacctgact gatagcccaa
     2341 tcggtccaac ggatagtttt ctccctgttt acatcaccgt tcagcaaagc atattcaaaa
     2401 cctaaattca gatcaacata atcgcttaag ctataagcaa aggaggttac aaaagctgtt
     2461 ttattcaggg aaccctcact ttctattttg tttaaggcaa ttttttccgg gtaaacatcg
     2521 ttatcggtat tgcgattgtt gcgaacttct tctctataat cggcatcaaa actggaaacg
     2581 ggtttataat agaaacccaa acccaaacct ctttccccaa aacgatgtgc aaaaaaagca
     2641 gagagagcat aattatcaaa ggtattgata ttagaggaat acacagaatc gtctatatag
     2701 ttatcaaagg aattatacaa gggaatactg cgattatcct cattccgatt cagataagta
     2761 ttaacggaaa aaccaagggt tttccgcatc aaagttaaat tagcaggatt atccgctatg
     2821 ccgaaaacac ctttactgtt gtataaaccg gctccgccca tagcaaaact gcgggcatca
     2881 aaagtggaaa aaggattgcc gaaataggta tcggttatgc tccaggcaga aagcgctgaa
     2941 gcaacaatta atagacatat aagtattaag gacttattag ataacattat tcctccatat
     3001 taataacggt agtcaatgct taagcgaatg gtattttcgc tgtgttcaac tctttgaaaa
     3061 tagacaggat catccaataa agtaggatca tccgaaagat tataattacg aatccggatt
     3121 tctttatcct gataggtttt attcttgaat ttcaaattta gatacatatt ttgtgaaacg
     3181 cgactggtaa aggcaatcca tgctttagaa cctttttcac ccataaaatc aatttccata
     3241 tcttcccaat cccaatgcga aataccatgc ccaaaccaat acaggaaaga accctgcatt
     3301 tttaacgatt tattgaaata gtgagtataa ttaaccgcta cataatcacc aatcattaag
     3361 gtttgagctg cagccattga attttcacct tcagccgcag gataggttaa ggaagtataa
     3421 ggcggacccc aaaccgtgtt ataacgatat tccagctcca gaaaatcacg attggaaaga
     3481 aaagttctga atgctaaagt atattcatta gttttggaaa cgcctcgttc cgcatcatca
     3541 tcataacgat taacctgatt tttataacgc aaacgcatag caagctgata aataggacga
     3601 aattccagtt cgctttgaaa tcttacactt ctgcgtccat cggttaagcg ttcccaaatg
     3661 tctaaatagc ttctattgat ggtatagtat ctgttaaatt tataacgggt ttcaaaatag
     3721 atgcctcgtt cgggttgtga ctgattagaa ttgagataaa ggtctgcaat ggtaggatta
     3781 gttaaagcat aaacattctt ttccagaatg gtatcttcaa agcgttcatg ctctgaaaaa
     3841 ctgttgctgt aaggattatc aaaatccaca tcataattac ggaacaaggt aataaaatag
     3901 agattttcaa actgggtgtg tgcagaaaca agatatgcct tagggtcatc cccaagtttc
     3961 caatcatccc cattaacagt aagttcagca tattcacctt gaatggaagt attcccaatc
     4021 acggtttgtc catcaaaacc aattactctt ctataatcgc gagtatattt attggtctga
     4081 gtgctataaa gattactgat ttcggcattc atatttttca ccagtttgga ataataatag
     4141 gaatccctta agaccgtcgg tagcaagtta ttataggtag gaaccacaaa atgagcatca
     4201 tcataaatag cagtataagt agtaaatcca agctttgtgc ctaaaaacgg attgatctgc
     4261 aaatgcgttc cccataattt ctcctgcaaa acatctttac gtgcagcaag attgatatag
     4321 ttcattgcat aagggacgcc agcagaaatt tcgttattga aatatgcttc tgcttctctc
     4381 attgtgtcat catcataacg caaagtggga ttgattaaag caaagacctt ggtcttatct
     4441 ccctgaacag cagtgctatc tgcataatta taaatatgct ctccattgtt atttatataa
     4501 tattgtctgc caaagtaatc agatgttgta tctctatcgg tataaatatc atttccatat
     4561 tcatcccgca taacagcata accatctcta tcaatatatg ttaaagcatc tttattgtca
     4621 acggatacaa aagcagtaac tccaaacaaa ggagttgtat attcaacggc tccgcctttt
     4681 aaagaatttt cctgcgttct ggaaaggtcg ggggttatgc ctaaaatcct tttgctaaaa
     4741 ccataaccgg ttttacgggc actgtaataa tccgtatttt ccatcaccaa accctctcca
     4801 aaagtagctc ggtagtttcc cgcataaacc tttaagcggg aattataaaa accaggcaat
     4861 tcgctatttt catatcctac ataccatttg gaatcggcta aaatatcctc tgccttgata
     4921 ttcagtaaat cctcttctcc tttggctttg taatgcatta tacccagttt atagttattg
     4981 ccgtaacgca tcctcagctt aagcataata tcgggatcaa gcttatccaa accaaaataa
     5041 ccccaatagg aattggtttt tagatgtgga atgacaacat tggtagcatt ataatcgttc
     5101 cgcagaaaac tttcgtgcaa catatcatac tgcccttctt caaaatagcg ggtataatat
     5161 aaaagcgaag catcacacat taatctgttt tttaccggtg gctctttata atatacataa
     5221 ctgcgcaagt ttgaataacc ataataggaa aggcctgtag aattgcgtaa gtcccttgta
     5281 tcggcaattg tatcaccctg ggcaattctt ttcaaaacag ctacagcatc aattccagaa
     5341 acattgggaa aactcatcaa atcgtcaaag agcattttat tcacattctg aggagtcatt
     5401 aaataatctt cccaaacatc tgccattcct tccgaagcac cttcattact gtctacacgc
     5461 tccagtaaat ccctgatttc ttcacggcgg actgctgctt catccgtttc tacataaata
     5521 gaaacaacaa ctaagggttt caaacggtca agggtctcct gatcaataga gggaattttc
     5581 cttagctcat agatatcgtc aaatatcttc acaaattcac gatattcata gatatctttt
     5641 gcctgctttt ctgttatggg taactgcatc aattcagcca aagatgctgt atttaaatcc
     5701 accttggctg ttaaaacaga tagccccgcc agaacaaaga gcattattaa aaacttcttc
     5761 attgctctac cttataagtt ttatactttc tgaaacttta ttttttatca tagatagcca
     5821 caataggaat agctataatt ctgtccagtt ttttttgctt tgctttttct aatttagaga
     5881 ttacattgtt tttaatactg gtttaccaag ctattaagaa taattctttt tatttcctgt
     5941 ctgccaaaat caaattggaa tttcaaaata tttgttatta gcttgaaaag gagttttctt
     6001 atagcttaaa gaattgaata aaatcatgtc attagcacat tattggtttg tcaaatagaa
     6061 aaatattata gaggtagaac taccattacg acaaggtttt ggattttatt ttataaaaag
     6121 cccttttttt catctatttt ttgcttgaca aagatagcag ggtgacttag tatatatttt
     6181 aggtaaagta gagtagtaga atgaaaaagc gtaaaagtat actaagttca ttaaacacac
     6241 tctgttataa gagagaggta atgttctgtt tctcggtttt aataagaaag tcactttatc
     6301 agggaggata aaatgcaaaa gaaatttttt gtttttttag tcgctttagt ttggtcaatt
     6361 gctttagtag gtgcttctgt tactttggat tcattagaaa tatctgttca aggtggaggt
     6421 taccccagac aaggatctac tattgttata actatgtatt atcgtgttgc tgatatacca
     6481 gaaggggaaa gtatttatgt ttatggtata gctacaggtg ctgttgacca agcacaaata
     6541 aaatggtatc caagttctaa tggtactgga actgcatcca atcaaaatgt atctgttaat
     6601 caagtagcta acacaattcc ttatgatgag gatggaaatg gatatcagag ttctcataca
     6661 gctactttta ctttaccagc accaccttca agttcttata agagttttaa aattaacggg
     6721 aaagtatacg cggcaacttc tgacgatgaa tttacatatt tctattccgg aataaagcta
     6781 tcaaaaaatt atttaacaat aaacaaccca cctgtggctt caagtgttgt tattgttgat
     6841 cccactttag ccagagtagg gcaaacttta actggagatt atgagattag tgatcctgat
     6901 ggtgatgaaa tagatacccc tatctttcgg tggttaagtt ctactataga aactggaact
     6961 tatactgcta tttccggtgc aacaggaaat acatatacag ttcaatctgc agatgcaaat
     7021 aaatatataa aatttgaggt aacacctatt gctaaaaccg gtgagctgca aggagcaccc
     7081 aaattaagtg atgctactcc aaaagttaca ggagctccaa ttgtagaaat tgatcccagt
     7141 acacttaata tatatgaagc aagtgctaat aatggttccc tgggtgatgc ctatgtaaag
     7201 attacttgta ccaataccag ttttaaatct tccattacta attccgctat tactatatca
     7261 aaagatttag acggattatc aaaaggcagc gtaacctgga ttagtgccaa tgagattcgt
     7321 cttagttttt caggtgcagc agtaaatcat gaatatgctg ccaaaataga tactatccgt
     7381 attactataa acaaagatca attagaaggt gtagaaaata acttaacaac ccagaatggt
     7441 tttaacataa actttaccaa taatccccct accaatttag ctttagatag cgttggcaat
     7501 aactatgtta aaataacctg ggataaacct gctggtctat taacgagtgg aagtggattg
     7561 gattatttca atattttaag aaatgaaact aatattggca gtctctatct tgataaaggt
     7621 aaaggaactt attattatac cgataatact gttaccaatg gaactcagta tcgttataaa
     7681 gttcaagctg tttatactgc agggacaaat gaaacttcta cagaactaag gggaactcct
     7741 cttgcgatta ctgcatattc tttcagcagt ccacctgcaa ccggaacaat tgatcatacc
     7801 aataagacca tcaaagtagt tgtccctaat ggaactgata gaaatgcttt agtagctaat
     7861 tttacagctc ccggggcaat tgtaaaggtt ggaaccgcta ctcagaccag tggctcaact
     7921 tcgaataatt tcactaatcc tgtaacctat actttaacta ccaataatga tgcttctact
     7981 tgttcttatg tagtaacggt aaatgagaaa ttagctactc cctctccttc tgtggtagga
     8041 tcaaatataa ccacttccag tattaaaatt cattgggata caattacagg ggcagataga
     8101 tatggtgtag atgtttcgga aaataacagt tttttacccg gcagttttca gcataaagag
     8161 gtatctgcta atttctgcat tatgaatggt ctgaaagcag gtaccaaata ttattaccgg
     8221 gttcaggcaa ttgctgagga tcaatatttt aacagtgatt attcttctgt ctatgatact
     8281 acaactgatg cattaattcc gggggcaggc agcacggaaa tagatagcag ttatgaaacc
     8341 acaataaatg taggagaata tgtttacggg caagaaacag taactcctac tgtgaaagtt
     8401 catcccgctg aatttagtcc ttctgaaaat gatatcatta cggtaactat gagttatgga
     8461 actactcctg agggtttgca gtataacctt gcttttgaga atcctactat tggtaatgga
     8521 acttttattc tttcctacag cggattgagc tatgatccca cagatgtagg ttatcgtttg
     8581 aatggtggtg ccttacattc ggtaggaggt gatggtataa atacaacaga taaaatcgtt
     8641 attttcacaa tgagtggctt gggtaaagga agtaaagcag cttatgaatt acaaattgtt
     8701 ctcaatgatg aaagtggtca gacattacct gttgttctta catcttttac cgctactcaa
     8761 atggttgacg gtaaagtgcg tgtggattgg gtaactcaat cggaaaccaa tctttcagga
     8821 tttcgtgttt tgcgcaatac agttgccgaa atttccacgg ctatggccgt aagtcctcta
     8881 attgaagcaa cgaatacttc agctactgtc gtttattcgt ttatggacag cgaagttccg
     8941 ggtaatggca tttactatta ctggttacaa attgaagatt tggatggagg tatatcctat
     9001 tctgctgtct atactgttca agtgactact ggtggagtta caaatcccat aataccaaaa
     9061 attactgctt tgcttaatcc ttttcccaat ccttttaatc ctgatgtaac tattcctttt
     9121 gatcttgctg tagatggcaa tgttacctta aaaatttaca atttgaaagg gcaattgatt
     9181 aaaaaccttt taaatgaaaa ttgcaaagca aattcttatc gtatcctttg ggacggcaag
     9241 gataacgagg gacatactgt ttctaccgga acttatatta ttcggatgaa tgcgcccaat
     9301 tatcaagcat cacataaaat ctccttgatt aaataaacta tattctccct gatctaacag
     9361 gtgtgtatct ctggtatatg cacctgttat ttttttgctc gtttttgcta ttctgcattt
     9421 atactttaag tttcttctgc aaaacccccg ctcttatttc tctaattttc ggaaaaattt
     9481 ggatgatgta tcttactaat aatatatggt attaggaggg tcttccttaa tagcattgct
     9541 tacccattgc ttacccattg ctaatggcat tagtaatggg taagcaatgc ttccgtaata
     9601 caccttggaa atcaagaaag ctattgacag a
//
LOCUS       DAOPFL010000066         9506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_8931_length_9506_cov_101.545657, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000066 DAOPFL010000000
VERSION     DAOPFL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8931_length_9506_cov_101.545657"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLG69_05485"
     CDS             complement(<1..277)
                     /locus_tag="PLG69_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPI42997.1"
                     /translation="MKFDYHIHTEDSYDSSIKAIDLINRAIQLNYDEIAITEHLDLLP
                     QEIKKYGAPSLDRYYKRIKELQNNTSELNVLCGIEIGDYHRVKEYALS"
     gene            complement(274..1038)
                     /locus_tag="PLG69_05490"
     CDS             complement(274..1038)
                     /locus_tag="PLG69_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPI42998.1"
                     /translation="MLKIGIDIGSRNTKIVIYDAQTKRIEFSAFQTTEVSVTDGVNNL
                     LKEGYTALGITRKINTIGVTGYGRKLYQEASSILSEISCHTAGCLYYFPHIRTIIDIG
                     GQDSKIITLNEKGKVTDFVMNDKCAAGTGRFLEMTAMRLGFDVSELSLLASKSTRNLT
                     LNSTCVVFAESEIIGMLSSSIPPEDIVRSVHRSIAKRILAQMSVMIWEQPLVFTGGVA
                     LNKDMGKCLSEALNSQLLTPPEPEITAALGAAILAK"
     gene            complement(1041..3704)
                     /locus_tag="PLG69_05495"
     CDS             complement(1041..3704)
                     /locus_tag="PLG69_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPI42999.1"
                     /translation="MNNRINSGLNNFPKPVSRILANGLEVISVQDNSNSVLCLQLYIR
                     TGSVQENKNQRGYSHFIEHLSFKSTKDFPFNGISLFASGLGGMLNAFTDYDCTCYYVN
                     LPAEKLKEGLHILSQLAFQSTFSREDVKTEKEIIVEEIKQYKNDPETDFLEYIQSSYY
                     QKSPLKYPILGSPESIMQADWEALHRFYKNRYIPENAFLIICGDFCQKELDYYLDYYF
                     TPWKSRGKPVKQLTNIEPEINGFRYFFRQKELNENTIAIALPELSEKHPYANALLIAI
                     RYLAIGKSSRLFKRLVEEEKICSSVKVSSLCGILSGASVISITPLSDKYISEVIKLFR
                     TEYSALLNYGIPESELELIKKDIINSWLFSFEGMENLASLVATEKFVGDLNRLQSYGA
                     EINATTLNDVKQAMYKYWLPEGLAVYYQGAVENVDFAEKAYALKSVSLQQNSNSSPAI
                     LSLKPSLNLEIKPLNQDVKINTEKITPIAEGFYQIILSNGMQVLFKQLKNKSISGFSL
                     STPISQICETTSTIGHNFFCSSLLLYKTQKHSHQELQQFSRENGFNIRLIHHLDTTTF
                     RGKCLSVNLKKALSMLAEIIYLPNFDRNYLSLLTSAALDEIRRDNDIPVSYAYLNWYK
                     MLVGNNSNLFRSSGNPSHIRSLHLKDIQEWYEKWNIGKDFYLGIVGNHKPEEVLELCE
                     QTFGLAKKASQSLYPKPLYSPSTIHFKRKYRKTDQAIIFNGGFACPAVSRDENTAFYV
                     LSQILGGDISSRFYYILREKYGYAYQTGFEFHSLNELGFWSAYAFCDRDDYRNCLTLM
                     QDILYSLTEKEVAEDELENAKQYLIGMNRFEDESVSYTASMMSNLSALGYEPEYYLSR
                     EERIRKVNPEIIQQIARKWLPPENQYIYLLL"
     gene            complement(3709..4482)
                     /gene="folE2"
                     /locus_tag="PLG69_05500"
     CDS             complement(3709..4482)
                     /gene="folE2"
                     /locus_tag="PLG69_05500"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPI43000.1"
                     /translation="MSIPDIQSQADNRKITIDKVGVKGIRYPIVVQDREKGIQHSVAD
                     LNIYVELPHQRRGTHMSRFLEVLNRYHTEVFIDKLDAFLSELKTTLKADSAYIDIMFP
                     FFIKKQAPVSRVSSLLGYDCSFNASFKDSFELWIGVKVPVTTLCPCSKAISVYGAHNQ
                     RSDIFIKVRYREFVWLEELIQLAEKHSSCEIYPILKRSDEKYVTEKAYNNPKFVEDVV
                     REITLELNKDKRITGFYVEAENFESVHNHNAYALISRNL"
     gene            complement(4479..5483)
                     /locus_tag="PLG69_05505"
     CDS             complement(4479..5483)
                     /locus_tag="PLG69_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPI43001.1"
                     /translation="MNRREIISLIILILVIGFGTYKYLTRSFTELKSQYLMDTIVEIS
                     ATSQSKNVGKQIDAVFNYIRNLEDKLNEFNPQSYLAKINTDTLNTVFEMDPDVYNLLT
                     IADSLYKMTEGAFDPTIKPVWDLWGFNAEKPVPPDSLEIKKMLEKVDFSKIKYTPQQL
                     FKPVGMQLTFGAIAKGYILDKAREYMQTLNLDSGFINCRSSMAFMGFQKPQIVYIQHP
                     RRLDDYIASFKVLNQSVSTSGDYQQFYEYQGVRYHHIINPFTGYPVPDVHSVTVLCPS
                     AAWSDGLSTALFLLPPETAISVIKPIPDCEAIIYYTKDDSLVSLKTKGMKKRDLNEKL
                     "
     gene            complement(5497..6294)
                     /locus_tag="PLG69_05510"
     CDS             complement(5497..6294)
                     /locus_tag="PLG69_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43002.1"
                     /translation="MRCKYCNARLAVHDIWCMQCGKQTPVIKNELYSMKSFSETWKAY
                     KSKMSSSLPGAGFAIILGLIPIAVLIWLFSGIIQLECNTATQMLLNLLIKSLAFSLFI
                     PFILIAFNPICKHNDYHLKLSEMFSALRSYPRYFLWTLINALYFCLIYIICFGLPNFA
                     SAPILRLVWLVLVNYWVAIALPSLVLMEEMSYNPWKAIKKSYQHFQDVRWNIYLLALV
                     LAILNALGCLFFWLLLIPEALILPLSLYALRDYTRRLIDFELLNYRL"
     gene            complement(6380..8635)
                     /locus_tag="PLG69_05515"
     CDS             complement(6380..8635)
                     /locus_tag="PLG69_05515"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPI43003.1"
                     /translation="MKLTENALKVLEKRYFRKDENGVLIEDWDKMLTRVASNIASGDE
                     EKTHRFYQLMDAGYFLPNSPTLMNAGGDLQQLSACFVLPIEDSMESIFETVKNAALIH
                     KSGGGTGFSFSHLREANARVRSTNGVSSGPLSFLKVFNAATDAVKQGGTRRGANMAIL
                     NVDHPQILDFITCKENPAELTNFNISVGITEDFMQAVANDDEYNLISPHTGVIYKKLK
                     ARDVFALIVKMAHKNGEPGIVFLDRLNNANPTPALGKIESTNPCGEQPLLPYEACNLG
                     SINLALLLKDGDVDWDKLKEVVRESVDFLDAVIDVSKFPLPQIEKISQANRKIGLGVM
                     GWADLLFQLKIPYASEEAVALAAKLMEFIDFYAKERSSELAQEKGAFPNFQESIYATG
                     YLKREKINQPWDSLKEKIKKEGIRNATLTTIAPTGTISMIADTSSGIEPQFSLAYVKN
                     VMDGEKLIYVNKYLQKALEEKGIYNNKLLEEILTRGSLAGIEGIPDDLKKVFQTAHDI
                     SPEWHIRMQAAFQKYTDNAVSKTINFPNSATVEDIQSAYELAYHLGCKGVTVYRDGSR
                     ESQVLYLGTQTTSPEKTDKKVAPRTRPEITKGITQRLETGCGHMYVTINTDAQGACEV
                     FIQMGKVGGCASAQLEAIARLVSLALRSQIKIESITRQLKGIRCQSPMWNKGKMITSC
                     GDAVGQALEQFVTLYTKGQIQAGELSVENENPGSHQSVGNGFALCPDCGNTIEHTEGC
                     LKCPACGWSKC"
     gene            complement(8647..9477)
                     /locus_tag="PLG69_05520"
     CDS             complement(8647..9477)
                     /locus_tag="PLG69_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPI43004.1"
                     /translation="MEPLILTAAITGAETTRADQPNLPITPEEQAKEAKACFEAGARV
                     IHLHIREDDGRPSQRLDRFQEAISAIRKAVPEIIIQISTGGAVGESFDKRLAPLALKP
                     EMATLNAGTLNFGDDIFINHPADIIRLAEAFKQYNVVPEVEVYESGMVDAVARLIKKG
                     IITQNPLHIQFVLGVPGGMSGKPKNLMYMMEHLKEEIPTATWAVAGIGRWHIPTSLIA
                     MVTGGHIRCGFEDNIFYHKGVIAESNAQLVARLARIAKEIGRPLATPEQAREILALNK
                     "
BASE COUNT         2975 a         1880 c         1744 g         2907 t
ORIGIN      
        1 aagagagggc atattcttta accctatgat agtcaccaat ttcaattcca caaagaacat
       61 taagttctga tgtattattc tgtaattctt ttattcgctt ataataacgg tctaatgaag
      121 gtgcaccata tttctttatt tcctgaggta gtaaatccag gtgttccgta atagctattt
      181 catcgtaatt tagttgaata gctctattaa ttaaatcaat agctttaatt gagctatcgt
      241 aactatcttc agtatgaata tgataatcaa atttcatttg gctaaaatag cagcgcccaa
      301 ggcagcggta atttccggtt caggaggggt aagtaattga gaatttaatg cttcacttaa
      361 acattttccc atatctttat tcagggcaac tccacccgta aaaacaagcg gttgctccca
      421 aatcataaca gacatttggg ctaaaatgcg ttttgctatg ctacgatgaa cagaacgaac
      481 tatatcttct ggaggaatag aagaagaaag catcccgatt atttccgatt cggcaaaaac
      541 aacacaggtg gaattaagcg taagattccg agtggatttg gaagctaaaa gagaaagttc
      601 ggaaacatca aaacccaaac gcatagctgt catttccaaa aatcttccgg ttcctgccgc
      661 acatttatcg ttcatcacaa aatccgttac tttacctttt tcgttcaggg tgataatttt
      721 agaatcctga cctccgatat caatgatagt tctaatatga ggaaaataat atagacaacc
      781 ggctgtatga cagctaattt ccgatagaat ggagcttgct tcctggtaaa gtttgcgccc
      841 atagccggta accccaatag tgtttatttt tcgggtaatt cctaaagccg tataaccttc
      901 ttttaaaagg ttgttaactc catctgtaac cgaaacttca gtggtttgaa aagcactaaa
      961 ttctatgcgt tttgtttggg catcgtagat aacgattttt gtgtttctgg agccgatatc
     1021 aataccaatt ttcaacataa ttacaacaat aaatagatat attgattttc aggtggcagc
     1081 cacttgcggg caatttgctg aattatttcc gggttaactt ttctaatccg ttcttctctg
     1141 gacagataat attcgggttc atatcccaaa gctgacaggt tagacatcat agatgcggta
     1201 taggaaacac tttcatcttc aaaacggttc attccgatta aatattgttt ggcattttcc
     1261 aattcatctt cagcaacttc tttttctgta agagaataaa gaatatcctg cattaaagta
     1321 agacaattac gataatcatc gcggtcacaa aaggcataag cactccaaaa accaagttca
     1381 tttaaggagt gaaattcaaa acctgtttga tatgcatagc cgtatttttc ccttaagata
     1441 taataaaacc gcgaggaaat atcccctccc aggatttgag atagaacata gaaagcagta
     1501 ttttcatcac gactaacagc aggacaggca aatccgccgt taaaaataat tgcctggtcg
     1561 gtttttctgt atttcctttt aaaatggata gttgaaggtg aatacaaggg ttttggatac
     1621 aatgattgag atgctttttt agctaaaccg aaggtctgtt cacaaagttc taatacctct
     1681 tccggcttat ggttacctac aattcccaaa taaaaatcct ttccgatatt ccatttttca
     1741 taccattcct gtatgtcctt aagatgaagg gagcgaatgt gagaaggatt tccggaactg
     1801 cgaaaaaggt tgctattgtt tcccaccaac attttatacc agtttaaata ggcatagcta
     1861 accggaatat cattatccct ccgaatttca tctaaagcag cagaagttaa aagagaaaga
     1921 tagttacggt caaaattcgg aagatagatt atttccgcca gcatagacaa tgcctttttc
     1981 agattaacgc ttaaacattt tcccctgaaa gtagtagtat ccaaatgatg aattaaacgg
     2041 atattaaatc cattttcccg ggaaaattgt tgtaactcct gatgactatg tttttgagtt
     2101 ttatacagca atagggaaga gcaaaaaaag ttgtgtccta tagtggaagt tgtttcacaa
     2161 atctggctga taggagtgga aagagaaaaa ccgctgatgc ttttgttttt cagttgttta
     2221 aagagaactt gcatcccgtt ggaaagaatt atctgataga agccctcagc tatgggagtg
     2281 attttttccg tatttatttt cacatcttga tttaagggct taatttcaag attgagagaa
     2341 ggttttaggg ataaaattgc cgggctggaa tttgaatttt gctgaagtga aacactttta
     2401 agggcataag ctttttcggc aaaatcaaca ttttcaacag ctccttgata ataaacagca
     2461 agcccttcgg gaagccaata tttatacata gcttgcttta catcgtttaa agtggtggca
     2521 ttaatttctg caccatagct ctgaagacgg ttcaggtctc ccacgaattt ttccgtagct
     2581 accaaactgg caaggttttc catcccttca aaactgaata accaactatt gataatatct
     2641 tttttaatca gttccagttc ggattcaggt attccgtagt tgagcaaggc agaatattca
     2701 gtcctgaata actttataac ctcagagata tatttatcgg aaagaggagt aatagaaatg
     2761 actgaggccc cggaaagaat tccgcaaaga gaacttactt tcacgctgga acaaattttt
     2821 tcttcttcca ccaggcgttt aaatagacgg gatgattttc ctattgccaa ataacgtata
     2881 gctattaaaa gagcattagc gtaaggatgt ttttcgctta attccggtaa agcaatggca
     2941 atagtatttt catttaactc tttttgccga aagaaatagc gaaatccatt tatttccggt
     3001 tcaatatttg ttagctgttt tacaggtttg ccccggcttt tccagggagt aaaataataa
     3061 tccaaataat aatccaattc cttttggcaa aagtcaccac agataatcag aaaagcattt
     3121 tccgggatat agcggttttt atagaaccga tgaagggctt cccaatccgc ctgcattatg
     3181 ctttcaggac tacccaaaat aggatattta agaggacttt tttgataata actgctttgg
     3241 atatactcca aaaaatcagt ttcggggtcg tttttatact gttttatttc ctctacaatg
     3301 atttcctttt ccgtttttac atcttcccgg ctgaaagtgc tctgaaaagc aagttgagaa
     3361 aggatgtgta aaccctcttt caatttttcg gcaggaagat ttacatagta acaagtgcaa
     3421 tcgtaatcgg tgaaagcatt tagcattcca cccagacctg aggcaaaaag ggaaattccg
     3481 ttaaaaggaa agtccttggt agatttaaag ctcaaatgct caataaaatg cgaataaccc
     3541 cgctggtttt tattttcctg aaccgaacct gttctaatgt ataattgcaa acaaaggaca
     3601 gaattggaat tatcctgcac ggaaataact tccagtccat tagctaaaat tctgctaacg
     3661 ggtttgggaa aattgtttaa ccctgaattt atcctgttat tcataatgtt ataagtttct
     3721 gcttatcagg gcataagcat tgtgattatg gacggattca aaattttcgg cttctacata
     3781 aaatccggtt attcgcttat ctttatttaa ttccagagtg atttcccgca caacatcttc
     3841 cacaaatttg ggattattgt atgctttttc ggttacatat ttctcatccg agcgctttaa
     3901 aatcggataa atctcacagc tggaatgttt ttcggcaagc tgaatcagtt cttccagcca
     3961 cacaaattcc cggtaacgga cttttataaa tatatccgaa cgctgattat gagctccata
     4021 aacgctgatt gctttagaac aaggacaaag tgtagttacc ggaactttta ctccaatcca
     4081 gagttcaaaa ctatccttaa aagaggcatt gaaactgcaa tcataaccca aaagagaaga
     4141 aacgcgggaa accggtgcct gtttttttat aaaaaaggga aacataatgt ctatataggc
     4201 agaatcagct ttcagggttg ttttcaactc acttaaaaaa gcatccagct tatctataaa
     4261 gacctcagta tgataacgat ttaagacctc cagaaaacgg ctcatatgtg ttccccgacg
     4321 ctgatgagga agttctacat agatatttaa atcggcaaca ctatgctgaa tgcctttttc
     4381 tctatcctgc actacaatag gataacgaat accttttaca ccaactttat ctatggttat
     4441 tttgcggtta tcggcttggg attggatatc aggtatactc ataacttttc gttcaagtcc
     4501 cttttcttca tccccttcgt tttcagggaa acgagagaat catctttagt ataataaatg
     4561 attgcttcac aatcgggaat cggtttaatt actgaaattg cagtttccgg aggcaacaaa
     4621 aaaagtgcgg ttgaaagacc gtcagaccaa gcggcagaag gacaaagaac agttactgaa
     4681 tgcacatcgg gaacgggata acctgtaaaa ggattgatga tatgatgata acgaacaccc
     4741 tgatattcat aaaactgttg ataatcaccg gaagtagaaa cactttgatt cagcactttg
     4801 aaactggcaa tgtaatcatc taagcggcga ggatgctgaa tataaacaat ttggggtttt
     4861 tgaaaaccca taaaagccat agaactgcga caatttataa atccgctatc caaatttaag
     4921 gtctgcatat actctcttgc tttatccaaa atgtaacctt tggcaatagc gccaaaagtg
     4981 agttgcatac ctaccggctt aaacagttgt tggggagtat attttatctt actaaaatcc
     5041 actttctcta acattttctt tatttcaagc gaatctggag gcacaggttt ttcagcatta
     5101 aaaccccata aatcccatac cggcttgata gttgggtcaa aagcgccttc agtcatttta
     5161 tataaactat ccgctatcgt gagcaaatta tatacatcgg ggtccatttc aaagaccgta
     5221 tttaaagtat cggtattgat tttagccaga tagctttggg gattgaattc gtttaattta
     5281 tcttccagat tgcggatata attgaaaaca gcatctattt gtttgccaac atttttactt
     5341 tgcgaagtgg cagatatttc tactatggta tccattaagt attgactttt aagttcagta
     5401 aaggaacgag tcaaatattt gtaggttccg aaaccaatca ctaaaatcaa aatgatgagg
     5461 gaaataattt ctctgcgatt cataaaatat aaaacctcat aaacgataat tcagcagttc
     5521 aaagtcaatt aagcgtctgg tatagtccct cagggcatat agggaaagag gcaaaatcaa
     5581 tgcttcggga attaaaagca gccaaaaaaa cagacaaccc aaagcattta agatagctaa
     5641 aactaaagcc agaagataga tattccagcg aacatcctga aaatgctgat aggatttttt
     5701 gattgccttc cagggattat aagacatctc ttccatcaaa accaaagaag gcaacgcaat
     5761 ggctacccaa taattaacta aaaccagcca aaccaaacgc aaaatcggag cagacgcaaa
     5821 attgggcaaa ccaaaacaaa ttatatagat aaggcaaaaa tataaggcat tgataagagt
     5881 ccaaagaaaa taacgcggat aactacgcag tgcggaaaac atttccgaga gcttaagatg
     5941 ataatcatta tgtttacaaa taggattaaa agcaattaaa ataaaaggaa taaaaagaga
     6001 aaaggcaagg gacttgataa gcaggtttaa taacatctgc gtagccgtat tacattccag
     6061 ttgaattata ccgctaaaaa gccaaattaa aaccgcaatt ggaatcaaac ctaaaataat
     6121 ggcaaaacca gctccaggta gggaagagga cattttgctc ttgtatgctt tccaggtttc
     6181 tgagaaggac ttcattgaat agagttcatt tttgatgaca ggtgtctgtt tcccgcattg
     6241 catacaccaa atatcgtgaa cagcaagtcg ggcattacaa tatttacaac gcatttatgc
     6301 tccttttttg ggggtctgct gaatttcgct ttagcaaata aagcaaaaaa taaaaaacac
     6361 tacttttgtc aagcacgggt taacatttac tccaaccgca ggcaggacat tttaaacatc
     6421 cttctgtgtg ttcaatagta tttccgcagt cagggcatag agcaaaaccg tttccaaccg
     6481 actgatgaga acctggattt tcattttcca ccgataattc gccggcttgg atttgtcctt
     6541 tagtataaag agttacaaat tgttccagtg cctgaccaac tgcatctcca caagaagtta
     6601 tcatttttcc tttattccac attggggatt gacaacggat acctttcaat tgacgagtaa
     6661 tggattcaat cttgatctga gagcgcaaag ccaaagaaac aagacgggca atagcttcca
     6721 gttgtgcaga agcacatcct cccacttttc ccatttgaat aaagacctca caggcacctt
     6781 gagcatcggt attaatagtt acatacatat gaccgcaacc cgtttccagg cgttgagtta
     6841 tgcctttagt aatttccggt ctggttctgg gagctacttt tttatccgtt ttttccggtg
     6901 aggttgtttg agttcctaaa tagagaacct ggctttctcg gctgccatcc cgatagacag
     6961 taactccttt acagccaaga tgataggcaa gctcataggc actttggata tcttccaccg
     7021 tagcggaatt tgggaaatta atggttttgc ttacagcatt atcagtatat ttttgaaaag
     7081 ctgcctgcat tctgatatgc cattcgggac ttatgtcatg agcggtttga aacacctttt
     7141 tcaagtcatc cggaataccc tcaataccgg caagagagcc acgcgttaaa atttcttcta
     7201 aaagcttatt attatatatt cctttttctt ccaatgcctt ctgcaaatat ttattcacat
     7261 agattaattt ctctccatcc attacatttt tcacataagc aagggaaaat tggggttcaa
     7321 ttccgctgga agtatcggca atcatactaa ttgttcccgt aggagcaata gtagtaaggg
     7381 tagcattgcg gattccctct ttttttattt tttcttttaa ggaatcccag ggttggttta
     7441 ttttttctct tttcaggtaa ccggtagcgt agatactttc ctgaaaattg gggaaagcac
     7501 ctttttcctg ggcaagttct gaagagcgtt ctttagcata aaaatctata aattccataa
     7561 gtttagcggc aagagcaacc gcttcttcac tggcataagg tatttttaat tgaaataata
     7621 agtccgccca gcccataact cccaatccga ttttcctgtt agcttgggaa attttttcaa
     7681 tttgaggtag gggaaacttg gaaacatcaa ttaccgcatc caggaaatca acactttcac
     7741 gcaccacttc tttcagttta tcccaatcca catccccgtc tttgagcagc aaagcaagat
     7801 ttatagagcc caaattgcat gcttcataag gcagcaaggg ctgttctcca cagggattag
     7861 tagattctat tttgcccagt gcaggagtgg gattggcatt atttagccgg tctaaaaaca
     7921 cgattcccgg ttcgccattt ttatgtgcca ttttcacaat cagggcaaat acatctctgg
     7981 ctttcagctt tttataaatc actccagtat gaggagaaat aagattatat tcatcatcat
     8041 tggcaaccgc ctgcataaaa tcttctgtta tacctacact gatattgaaa ttggttagtt
     8101 ctgcgggatt ttctttgcag gttataaaat ccagaatttg aggatgatct acatttaaga
     8161 ttgccatatt agcgcctctt ctggttccac cttgtttaac agcatcggta gcagcattaa
     8221 aaacctttaa aaaggataga ggtccgctgg aaacaccatt agtagaacga actcgggcat
     8281 tggcttcgcg taagtgactg aaagaaaaac cggttccacc tccgctctta tgaattaaag
     8341 ccgcattttt aaccgtttca aaaatgcttt ccatactatc ttcaataggc aaaacaaaac
     8401 aggcggagag ctgttgtaaa tcaccaccag cattcattaa agtaggagaa ttgggtaaaa
     8461 aataacccgc atccattaac tgataaaaac gatgggtttt ttcctcatca ccgcttgcaa
     8521 tattggaggc aacgcgagtg agcattttat cccagtcctc tatcaaaacg ccattttcat
     8581 ctttacgaaa atatcttttt tccaaaacct tcagcgcatt ttccgttagt ttcatagcca
     8641 ccccttttat ttatttaatg ccaaaatctc tcttgcttgt tccggagtag ccagagggcg
     8701 accaatttct tttgcaatgc gtgctaaacg agctaccaat tgcgcattag attcggcaat
     8761 tactccctta tgataaaaaa tgttatcttc aaatccacag cggatatgac cacctgtaac
     8821 cattgctatt aaagaggtag gaatatgcca tctgccaatt cctgccacag cccaggttgc
     8881 cgtagggatt tcttctttca aatgttccat catatacatc aggtttttgg gcttgccact
     8941 cattcctccg ggaacaccaa gaacaaattg aatatgcaaa ggattttgag ttataattcc
     9001 ttttttaatt aaacgagcaa ctgcatcaac catacccgat tcataaactt caacttcagg
     9061 aactacatta tattgtttga atgcttctgc caaacgaata atatcggcag gatgatttat
     9121 gaaaatatcg tcaccaaaat ttaaagttcc ggcattcagg gttgccattt cgggttttaa
     9181 agccaaagga gcaagtcgtt tatcaaatga ttcgccaact gcacctccag tactgatttg
     9241 aataataatc tcaggaactg ctttgcgaat agcagaaatg gcttcctgaa agcgatccaa
     9301 acgctgagaa ggtctgccgt catcttccct aatgtgtaaa tgaataactc ttgctccggc
     9361 ttcaaaacaa gcttttgctt cttttgcctg ttcttcagga gttataggca aattgggttg
     9421 atcggcacgg gttgtttcag ctcccgtaat ggcagcagtt aaaattaagg gttccataat
     9481 gttacctctt tatatttaat ctattt
//
LOCUS       DAOPFL010000067         9293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_9263_length_9293_cov_80.747889, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000067 DAOPFL010000000
VERSION     DAOPFL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9263_length_9293_cov_80.747889"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLG69_05525"
     CDS             complement(<1..763)
                     /locus_tag="PLG69_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPI43005.1"
                     /translation="MDDNKLTPMLKQYYAVKEKHPDKLILFRMGDFYETFFEDAHTAS
                     RILNITLTTRNKNDENPVPLAGFPYHALNTYLDKLIKAGLKIAICEQTEDPKKAIGLV
                     KREVTEIITPGAVLDQSFLEGNANVFLASLYYNDPQKKIGLAHLDISTGDFLFTELDK
                     EELVNELQRFRAAELIVDSTQAEEFVKTLPLETLPTITVFDSWQFQPQEAIATLKKHF
                     GVTTLEPYGAHNKLLGATAAGAALAYVQGLYNSPLN"
     gene            complement(766..1938)
                     /locus_tag="PLG69_05530"
     CDS             complement(766..1938)
                     /locus_tag="PLG69_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPI43006.1"
                     /translation="MLKSPFPAVKQVFLFILLTGLCCFAFAAVRKEFQDLDKLYNSGK
                     LDELSANISTLKPVNDEERASLGFYNALLKVKIEDAISAHQWLIEKFPKSPYAQKSLL
                     ELGKIFILDRKIEEATLYLRRITSPEIIERFYWLGLCAWWNDDYANAISYCENYLRLA
                     PRGEFAEKSYYLIAECYLEQKKAYSAVATLLKLQNAKLPEMDEQYFYYRLGYAYELSD
                     KPIDAITAYRKGYEQDPYSQVAYQIEDRILELKSSKPSIDISFLYPYSPLQVTIVQEE
                     QPDTLKTSVTTDSKSSSSSTAKQIEVKQDTPVKLKEKPKEGYWLQAGRFSVESNANRL
                     VINIRLLNIPAAYYEDVSGGKKSWVVVCGSFPDRAKAEEAKELLATNNINSFITAY"
     gene            complement(1938..3197)
                     /gene="rho"
                     /locus_tag="PLG69_05535"
     CDS             complement(1938..3197)
                     /gene="rho"
                     /locus_tag="PLG69_05535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPI43007.1"
                     /translation="MLFEEDLFSLNQLQLIRLAKKIGLPGYTQYKGTELIFKMLEFQA
                     AQEGLAFVTGCLEIMEDGFGFLRFPQNNYTAGRDDVYVSLTQIKRFGLKTGNMVSGPV
                     RSPKEGEKYYALLRVESVNNMAPDCMQNLRPFDELTPYYPTERLNLEFDPDNYSTRII
                     NLFTPIGKGQRGLIVAAPRTGKTTLLQDTANAILSNHPEVYLIVLLVDERPEEVTEMK
                     KILKPGSREVISSTFDESPKNHSAVSEMVLEKAKRMVELHQDVVIVLDSITRLARAYN
                     NITPSSGKVLSGGIDANGLIKPKKFFGSARKTEEAGSLTIIATALIDTGSKMDQVIFE
                     EFKGTGNMELVLDRTISDMRLYPAIDLVKSGTRREELLLTQNELNRMYVLRKYLKTIS
                     PIQGIETLRKKMQATQTNEELLNSMSN"
     gene            complement(3238..3951)
                     /locus_tag="PLG69_05540"
     CDS             complement(3238..3951)
                     /locus_tag="PLG69_05540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPI43008.1"
                     /translation="MNEIIKILSKVDGGNVLDAATGKGDFITTLKQHLKSYTQIIGVD
                     SSQRCVDYAQKIFPENNIEIFRMNLEDMQFEDNYFDMVCMSDALHHIKNREKVLNEMI
                     RVLKPEGLMLITEMHCDGEQTPAQETHIIMHHWVASVDRLLGTYHQKTFTRKELQDII
                     KKMPLKKVQITDFYVPVDDPSKNCPTLLRNCQEIIKRLQTMENTEALIKEGQELMQRI
                     KDIGYASACRLAITGYKKK"
     gene            complement(4084..4980)
                     /locus_tag="PLG69_05545"
     CDS             complement(4084..4980)
                     /locus_tag="PLG69_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPI43009.1"
                     /translation="MKEKATLILGGGSALGLAHIGVLSVLEEQYEITGIIGSSMGSII
                     GGLYSSGLNSEEIYKIAKSFKNTRIFSPLNLDRTIQGIFDGKSLLKAFLEWTEGKKIE
                     EGKIPFIACAYDLISKSTVLFNKGLYADAMRASTSLPVLFAPYKFKQYLFIDGGIEHP
                     LPLAFSSVLSQDKVIAVNVLPEAQKSACFINLESDEITKSKRLGRTEVIFNAVLQNQA
                     YLAIRDIAAFSPDIVIEAGMADGHPFAFHKARDFYNYGRKQTIETLANYNEPRFITAI
                     RKHYRNLIRYLPNLNIPVKDNT"
     gene            5254..6021
                     /locus_tag="PLG69_05550"
     CDS             5254..6021
                     /locus_tag="PLG69_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI43010.1"
                     /translation="MIKVENLSKVFTKKDGTNFYAVDKINFTAGDGEIVCLLGVNGAG
                     KTTTMRVLSTIFQPSEGTAEIQGWDIKKHPNKVRENIGFLSGDTGLYDRLSGREFITY
                     FGRLYSIADNIIKQRISEMATLLDMNEFLDKKIEFLSSGMKQKVSIVRSIIHYPPVMI
                     FDEPTAGLDILTSRNIISFMRDCKKRGKCVLFSTHIMREAERLADRIVLIHKGKILAD
                     GTLEDLRNTSSQTDLDDIFVYYINQYTDETELRANEF"
     gene            6011..8023
                     /locus_tag="PLG69_05555"
     CDS             6011..8023
                     /locus_tag="PLG69_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HPI43011.1"
                     /translation="MNFKHSLVIYRKELMEVLRDKRTIFTTFILPIILYPLIIVGFNS
                     IMIRQTKSLEERGATIAVQDSVNNNISRQLIQDLTKIKNYTIIPYSETTSRLYEDKDI
                     QCIITIRDSLGSDGTQFFKVYIQYDKSKEQSRMVFNKLSEQLSKTEKELQKELLQSFG
                     ISPDFLNLIDIRERDTSSAQKKMGMLLGMFLPYIVIMMLFAGASIVAADLVAGEKERK
                     TLETLLVSSVGRLEIVCGKYLTIITLAMLNMIINLFSISFSLKFMLANQSTEMAGVSL
                     PLNAILILLIAMIPLATLFAAILLSISTFSRNIKEARSYEQPLLIIAMLLGMVSFFPA
                     IEINNLMALIPIVNIALLFKGVLINEFTISQILLTIFSTVVLDVIAIIITIKLFNTES
                     VLFRTEEESGGLKVFKRKPKTFFNPYNGILYFSIALIVLYYLGSYWQAKDLFSGLIQT
                     EIIIIALPVLLILRLLHLNPKEVLRLKNPNWNSFLLIPFIAVSAQIIVSIISQLINIV
                     FPFPEKYLEALSQLYKMNETPWKVFMAIALLPGICEELLFRGFIIRFFEKYSVRWAVV
                     ISAILFAAYHLDPFRFVPVLLLSLLLGYLAIRSGSIYTSMFSHIIINGMAFVLVTYSN
                     TGWVKFIAKDGENLNYWLIVPALIIFCLSLYAFHKVTAKGEEKCVE"
     gene            8011..>9293
                     /gene="glmS"
                     /locus_tag="PLG69_05560"
     CDS             8011..>9293
                     /gene="glmS"
                     /locus_tag="PLG69_05560"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPI43012.1"
                     /translation="MCGIVGYIGHRNALPIVVEALKRLEYRGYDSSGCALIHDGELQV
                     YKRAGKIIELERCLPEPNKCTGNLAIAHTRWATHGEPNEVNAHPHLDCKGEIAIVHNG
                     IIENYKLLRDKLIELGHTFVSETDTEVIVHLIEQFLLSEPTLEDAVRSAMTKVEGTYG
                     LVVMSLKEPDKLIAVRKGSPLIIGIGENEHFITSDVNAIIIHTKRVIYLQDDELCVVK
                     ADGFEITTLDKRSVKPEISVVDWDISAIEKGDFKHFMLKEIFEQPVTIENAFRGRINQ
                     HLGTARLGGLGIPNFELRKIKQIHLVACGTSFHAALIGKYIIEDIARIPVFAEYASEY
                     RYRNPIIPEDTLVFVISQSGETADTLGALREAKAKGARVLAITNVVGSTIARESDGGT
                     YIHAGSEIGVASTKAFTSQVTIMTLLAILLGRMNYL"
BASE COUNT         2775 a         1684 c         1750 g         3084 t
ORIGIN      
        1 gattcaaggg agaattgtaa agtccttgca cataagctaa tgctgcacct gctgctgtag
       61 cccccaaaag tttattatga gctccgtagg gttctaaagt ggtaactcca aaatgttttt
      121 ttaaagttgc aattgcttct tggggttgaa actgccagct gtcaaaaaca gtaatcgtag
      181 gcaaggtctc taaaggcagg gttttaacaa attcctccgc ttgcgtgctg tctacaatta
      241 attcggcagc cctaaatctt tgtaattcat ttaccagttc ttctttatcc agttccgtaa
      301 aaagaaaatc accggtagaa atatccaaat gtgctaagcc aattttcttc tgagggtcat
      361 tataatataa ggaagctaaa aaaacattgg cattaccttc cagaaaggat tggtctaata
      421 cagcaccggg agttataatt tcagttactt cacgctttac cagtccaatt gcctttttgg
      481 ggtcttccgt ttgttcacaa atggcaattt tcagcccggc tttaatcagc ttatctaaat
      541 atgtatttaa agcgtgataa ggaaaacctg ctaaaggaac aggattttcg tcattcttat
      601 tccgcgtagt gagagttata ttcaaaattc tggatgctgt gtgtgcgtcc tcaaaaaatg
      661 tttcataaaa atcgcccatc cgaaaaagaa tgagcttatc cggatgcttt tctttcacag
      721 cataatactg tttcagcatt ggagtaagct tgttatcgtc cacttttagt atgccgtaat
      781 aaaggaattg atattatttg ttgccagaag ttcttttgct tcttctgctt tagctctatc
      841 tggaaaagaa ccgcagacaa caacccagct tttcttccct ccgcttacat cttcataata
      901 ggcagcagga atattgagca gacggatatt tattactaaa cgattagcat tgctttctac
      961 agaaaacctt cctgcctgta accagtaacc ttctttgggt ttttctttta atttaaccgg
     1021 agtatcctgt tttacctcta tctgttttgc agtagatgaa gaggaggact tggaatcagt
     1081 agtaacactc gtctttaatg tatctggctg ttcttcttgc acaatagtaa cttgcaaagg
     1141 tgaataaggg taaaggaaac taatatctat actcggttta ctgcttttta gctctaaaat
     1201 acggtcttca atttgatagg caacctgtga ataggggtct tgttcatagc ccttacgata
     1261 ggcagttatg gcatcaattg gtttatcaga aagttcatag gcatagccca aacggtaata
     1321 gaaatactgt tcatccattt cgggaagctt ggcattttgc aattttagta aagtagcaac
     1381 tgcgctgtat gccttttttt gttccagata acattctgca atcagataat atgatttctc
     1441 ggcaaattca ccacgaggtg ctaatcgcaa ataattttca caataactga tggcatttgc
     1501 ataatcatca ttccaccatg cacataaacc aagccaataa aagcgttcta tgatttcggg
     1561 agaagttatg cgcctcaaat ataaggttgc ttcttcaatt ttgcggtcaa ggataaatat
     1621 ttttcccagt tccaagagag atttctgagc ataaggactt ttggggaatt tttctattaa
     1681 ccactgatgt gccgatatgg catcttcaat tttcactttc agcaaagcat tataaaagcc
     1741 caaagaggca cgctcttcat cattaacagg tttcagggtg ctaatgttgg cggatagctc
     1801 atctaatttt ccactgttat aaagtttgtc taaatcttga aattcctttc taactgcggc
     1861 aaaagcaaaa caacataatc ccgtcagcaa aatgaaaagg aaaacttgct taacagctgg
     1921 aaagggactt ttaagcatca attgctcatt gaatttagta attcttcgtt ggtttgggtt
     1981 gcctgcatct ttttccgcaa tgtctctatg ccctgaatcg gagaaatggt ctttaaatat
     2041 ttacgcaaca catacatccg gttcagctca ttttgcgtta aaagtaattc ttcacgtcgg
     2101 gttccacttt tcacaaggtc aattgctgga tacaatctca tatcactgat agtgcggtct
     2161 aaaaccagtt ccatattacc tgtgccctta aattcttcaa agataacctg gtccatctta
     2221 ctgccagtat caattaaagc tgtggcaata atagttaagc tacctgcttc ttcagtttta
     2281 cgagcagaac cgaagaactt tttgggctta attaatccgt tggcatcaat tccaccactt
     2341 aaaacctttc cgctggaagg ggttatatta ttgtacgctc ttgccagacg agtgatacta
     2401 tccaaaacaa taactacatc ctgatgcagt tctaccattc ttttcgcttt ttccaaaacc
     2461 atttcgctaa ctgcggaatg atttttgggg gactcatcaa aggtggaact aataacttct
     2521 ctgcttccag gtttaaggat ttttttcatt tcggtaactt cttcagggcg ttcatcaacc
     2581 agcaaaacaa tgagataaac ttcaggatga ttacttaaaa tagcatttgc cgtatcttgc
     2641 agtaaagttg ttttccctgt tcggggagct gccacaatta aaccacgctg tccttttcca
     2701 atgggtgtaa aaagatttat tattctggtg ctgtaattat caggatcaaa ttccagattt
     2761 aatcgttctg taggataata aggggtaagt tcatcaaaag gacgcaaatt ctgcatacag
     2821 tcaggagcca tattattaac tgattccacc cttaaaagag cataatattt ttctccttct
     2881 ttgggactgc gaacaggacc actgaccata tttccggttt taagtccgaa gcgttttatc
     2941 tgcgttagcg atacatatac atcatcgcga cctgcagtat agttattttg gggaaagcgt
     3001 aagaagccga aaccatcttc cataatttcc agacaaccag ttacaaaagc gagcccttct
     3061 tgagctgcct gaaattccag catcttgaag attagttccg ttcctttata ttgtgtatag
     3121 ccaggcaaac caattttttt tgccaggcga attagttgaa gttgattcaa gctaaacaga
     3181 tcttcttcaa acagcattcg ttatctcctt tatcagctat tcgttatagc attataatta
     3241 cttttttttg taaccggtga tagccaaacg acaagcactg gcataaccga tatctttaat
     3301 gcgttgcatc aattcctgtc cttctttaat taatgcttcc gtgttttcca ttgtctgcag
     3361 acgtttaata atttcctgac agttacgcaa caaagtagga caattcttgc tgggatcatc
     3421 tacaggaaca taaaaatccg ttatctgaac ttttttcagg ggcatttttt taattatatc
     3481 ctgcagttct ttgcgggtaa atgtcttttg atggtaagtt cctaaaaggc ggtctaccga
     3541 ggcaacccaa tgatgcatta taatatgtgt ttcctgagca ggggtctgtt cgccgtcaca
     3601 atgcatttcc gtaattaaca tcaaaccctc aggttttaaa actcttatca tttcatttag
     3661 gactttttcc ctgtttttaa tatggtgtaa ggcatcagac atacagacca tatcaaagta
     3721 gttatcttca aactgcatat cttccaaatt catacggaaa atttctatat tgttttccgg
     3781 aaatattttc tgggcataat ccacacagcg ttgcgaggaa tcaactccaa taatttgggt
     3841 gtaggatttc agatgttgtt tgagggttgt aataaagtcg ccttttccgg ttgctgcgtc
     3901 taaaacatta ccaccatcta ccttggatag aattttgatg atttcattca taacagaact
     3961 cctcgcttgt attttgatat gggatttttg cctctatttt ttcttctctg attgtgcagt
     4021 tctgaggact gcgtttttat tcttaatcta taattactat aattttatgc ctgccatttt
     4081 ccgtcaagta ttatctttaa cagggatgtt taaattgggt agatagcgga ttaaattacg
     4141 gtagtgcttc cggattgcag ttataaacct tggttcgttg taattggcaa gggtttctat
     4201 tgtctgtttg cgaccataat tataaaagtc ccttgcctta tgaaatgcaa aaggatgacc
     4261 atctgccatt cctgcttcaa ttacaatatc aggactaaag gctgctatat cacgaattgc
     4321 cagatatgcc tgattttgta aaactgcatt aaagattact tcagttctac ctaaacgctt
     4381 ggatttggtt atttcatcac tttccagatt gataaaacag gcactttttt gggcttccgg
     4441 caaaacatta accgcaataa ctttatcttg ggaaagtaca gaagaaaaag ccaatggcag
     4501 aggatgttca attcccccgt caataaaaag atactgctta aatttgtaag gagcaaaaag
     4561 aacaggcaag gaagtagaag ctctcattgc atcagcataa agtcctttat taaaaagaac
     4621 ggtactttta ctgattaaat cgtaagcaca agcaataaaa ggtattttgc cctcttctat
     4681 ttttttgcct tctgtccatt ccaaaaacgc tttcagtaat gacttgccat caaaaatgcc
     4741 ttgtatggtt cggtctaaat ttagcggaga aaatattctt gtgtttttga aacttttggc
     4801 aatcttatat atctcctcag aattgagccc cgaggaatat aaaccaccta ttatgcttcc
     4861 catactggaa cctattatac cggttatttc atattgttct tccaaaacac ttaaaacgcc
     4921 aatatgagct aaacccaatg cagaaccgcc acctaaaatg agagttgcct tttctttcat
     4981 atttcatcta tccccaattt tttaccttaa agctaaataa agctaaatag tcaagcttta
     5041 tttggttttt atatcattcc cttttttgta aaattctttt gtccttccgg taaaagtatg
     5101 aattgggata ttttttttgc ctatataact gaattttgaa tgtggaattt atattagtta
     5161 gcgatatttc tttaccgaaa aggaaataaa aaacctcgac agaaatttaa ccttgcaaaa
     5221 ttgtgctttg gtaatccgat aaggggaaag tgtatgatca aagtagaaaa cttaagcaag
     5281 gtctttacca aaaaagacgg aacaaacttt tatgctgtag ataaaataaa ttttacagcg
     5341 ggtgacggtg aaattgtttg tcttttaggt gttaacggag ccggaaaaac aaccacaatg
     5401 cgtgttcttt ccactatttt tcagccctcg gaaggaacag ctgaaattca gggttgggat
     5461 attaaaaaac atcctaataa agtgagagaa aatattggtt ttctttctgg tgatacagga
     5521 ctttatgatc ggttaagcgg aagggagttt attacttatt tcggtcgtct ttacagtatt
     5581 gcagacaata ttatcaaaca gcggatcagc gaaatggcta ctttactgga tatgaatgaa
     5641 tttctggata aaaagataga atttttgtcc tccgggatga aacaaaaggt atctattgtg
     5701 cgttctatta ttcattatcc tccggtaatg atttttgacg agccaacagc cggcttggat
     5761 attttaacct ccagaaatat tatctctttt atgcgggatt gtaaaaagcg cgggaaatgt
     5821 gttttatttt ctacacacat tatgagagaa gcggaacggt tagctgatag aatcgtgcta
     5881 attcataaag ggaaaatttt agcggacggc actctggagg atttacgcaa tacatcttct
     5941 caaactgatc tggatgatat ttttgtttat tacatcaatc aatacaccga tgaaacggag
     6001 ttgagagcaa atgaatttta aacactcgtt ggtaatttac agaaaagagc tgatggaagt
     6061 tctgcgtgat aaaagaacca tttttactac tttcatcctt cctattattt tatatccttt
     6121 aatcattgtc ggttttaatt caataatgat ccgtcaaacc aaatcactgg aagaaagagg
     6181 agctactatt gccgttcaag atagcgtaaa taataatata tcccgtcaat tgattcagga
     6241 tttaacgaag attaaaaatt ataccattat accatacagc gaaactactt cccggcttta
     6301 tgaagataaa gatattcagt gcatcattac cattcgtgat tctctgggca gtgacggaac
     6361 gcaatttttc aaggtttata ttcaatatga caaatccaaa gagcaaagca gaatggtctt
     6421 taacaagctt tccgaacaac tttctaaaac ggaaaaggaa ttgcagaaag agttgttaca
     6481 gagttttggt atcagtccgg attttttgaa cctgattgat attcgggaac gcgatacttc
     6541 cagtgcgcaa aagaaaatgg gtatgctctt gggtatgttt ttaccatata ttgttattat
     6601 gatgttattt gcgggtgcat ctattgtagc tgcggatttg gtagcgggag aaaaagaaag
     6661 aaaaacattg gagaccttgc ttgtgtcttc agtagggcgt ttagaaattg tttgtggtaa
     6721 atatttaacg atcattaccc tggcaatgtt gaatatgata ataaaccttt tcagtatcag
     6781 tttttcccta aaatttatgc tggctaatca aagcacagaa atggcagggg tttccttacc
     6841 ccttaatgct atattgattt tgctgatagc tatgattcct ttggcaactt tatttgccgc
     6901 tattctgtta tccatttcca ctttcagcag gaacatcaag gaggcaagaa gttatgaaca
     6961 gcccttattg ataatagcaa tgcttttggg aatggtaagt ttctttccgg caattgaaat
     7021 aaacaatctg atggctttaa ttcctattgt taatattgct ctgctgttta aaggtgtttt
     7081 gatcaatgaa tttactattt ctcaaatact tttaactatt ttcagcactg ttgtattgga
     7141 tgttattgct attattatta cgataaaact gtttaacacg gaatcagttc tctttaggac
     7201 agaagaagaa agcggtggtt tgaaagtttt taagagaaag cccaaaacct ttttcaatcc
     7261 ttacaatgga attctctatt tttctattgc tctaattgtg ttatattatc ttggcagtta
     7321 ctggcaggca aaagaccttt tcagtgggct gattcaaacc gaaattataa tcattgcatt
     7381 accggttttg ttaattttaa ggttattaca cctaaatccc aaagaagttt taagattaaa
     7441 aaacccgaat tggaatagct ttctcttaat tccttttata gcagtttctg cccaaataat
     7501 tgtgtctata atttcgcagt tgattaatat cgtttttccc tttccggaaa aatatctgga
     7561 agcactttcc caattatata aaatgaatga aaccccttgg aaggttttta tggcaatagc
     7621 tttattgcct ggaatctgtg aagaactgct atttcgggga tttataatcc gcttttttga
     7681 aaagtattca gtgcgctggg ctgttgtaat cagcgcaatt ctttttgctg cctatcatct
     7741 tgatcctttt cgttttgtgc ctgttttgct tttgagtttg ttattaggtt atttggctat
     7801 ccgttccggc tcaatttata caagtatgtt ttcgcatatt atcattaacg gaatggcttt
     7861 tgttctggta acttacagca atactggatg ggtaaaattt atagccaaag atggagaaaa
     7921 tctaaattat tggctaattg ttccggcttt gattatattt tgtttatctc tttatgcatt
     7981 tcataaagtt actgccaaag gagaagaaaa atgtgtggaa tagttggtta cataggtcat
     8041 cgtaatgcac tgcctattgt tgttgaagca ctaaaaagat tggagtatag aggatatgat
     8101 agttccggat gtgctcttat tcacgatggc gaattacagg tttataaacg agcaggcaaa
     8161 attattgaac tggaacgctg tttgccagaa cccaataaat gcaccggcaa tttagccatt
     8221 gcacatacca gatgggcaac gcatggagaa ccaaatgaag taaatgctca tccacatttg
     8281 gattgtaaag gtgaaattgc tattgtccat aatggtatta ttgagaatta taaactcttg
     8341 agagataaat taatagaact aggtcatact tttgtaagcg aaactgatac tgaggttatt
     8401 gtccatctta ttgaacagtt tcttttaagt gaaccaactt tggaagatgc tgttcgctct
     8461 gctatgacaa aagttgaagg cacttacggg ctggtagtta tgtctttaaa agagccggat
     8521 aagctgattg cagtgcgtaa aggtagtcct cttattattg gtattggtga aaatgaacat
     8581 tttataacca gcgatgtgaa tgctataatt attcatacaa agcgagttat ttacttacag
     8641 gatgatgaat tatgcgttgt taaagctgac ggttttgaaa ttacgacctt ggataaacgc
     8701 agtgtaaaac ccgaaatttc agtggtggac tgggatattt ccgctattga aaaaggtgat
     8761 tttaagcact ttatgctgaa agaaattttt gaacaaccgg taactattga aaatgctttt
     8821 cgcggcagaa taaatcaaca tttgggaaca gcgcgtttag ggggcttggg aattcccaat
     8881 tttgaattga gaaaaataaa acaaattcat ttagttgctt gtgggacttc atttcatgct
     8941 gctttaatag gtaaatatat tattgaagat atagctcgca tacctgtttt tgcagaatat
     9001 gcctcagaat atcgctatcg gaatcctatc attccggaag acactttggt ctttgtaatc
     9061 agccagtcag gagaaacagc agataccctt ggagctttaa gagaagccaa agcaaagggt
     9121 gccagagtgt tagccataac caatgtagtc ggtagtacaa tagccagaga aagcgatggc
     9181 ggaacttata tccatgcagg aagtgaaatt ggagtagcca gtaccaaagc atttacttcg
     9241 caagttacga ttatgacctt gctggcaatt ttattgggaa gaatgaatta tct
//
LOCUS       DAOPFL010000068         9174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_9428_length_9174_cov_78.503893, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000068 DAOPFL010000000
VERSION     DAOPFL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9428_length_9174_cov_78.503893"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2018
                     /locus_tag="PLG69_05565"
     CDS             <1..2018
                     /locus_tag="PLG69_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPI43013.1"
                     /translation="GPHSYYVTAMYGTVESGASNTVNTFIIPPQHYELSYDDGTAEQG
                     FSVGVTRQMAVKFNYFGEFDLQHIKVYVHTLGTASIIIRIHDASGVNGMPGDLYCLYH
                     YPATSLVVGWNFITLPTPVHFNNQSFYVGILETTGASQIGLDTSSFGHSYKNISGSWE
                     LIGTGEIMLRAIISSDQIDPFGEPITLSTSMTVVCGATINGVQASQGDVLAAYVNVNG
                     SPQLRGKQTVMVQNNIAGCILQVYTEVNGENVTFKIWDQSANQVYSTSNVLPTTVNGT
                     IGSWPDNLYQINGIGYTTQNIPLVSGWNLVSLNVSPYDHSPGSLFAPLIGQVQQVKGT
                     EGIYIPNNPYSTLTALTDGKAYSILMSSPVTWSVTGIQIPASTPIPLVNGWNMAAFLP
                     SNSLSVTTAMSSISSWLIQLKGTDGVYIPSNPYSTLNTMYPGKGYWININGAHNLIYP
                     GKIASAEGIRSVTISPAKEQEAVKMLSNSFTLLAKCPEAEVGDYLLARVNGELRGKEL
                     LIAPEGFLAALIQVYSDIMGEEVRFSLWKPSGMEVALQNTISSEPGAIIGNYPNFIEL
                     YIEPLTTEEHIAILTCLQGVYPNPFNPSTTISFSVSEDNSPVKITIYNIKGQKVRCLV
                     QENFPCGEYKIIWDGLNDHGTTVSSGVYFAEMRTPGYRKQIKLLLTK"
     gene            2039..5386
                     /locus_tag="PLG69_05570"
     CDS             2039..5386
                     /locus_tag="PLG69_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43014.1"
                     /translation="MKIRILILSVCILMLSSVLLAQEMVPLSNLKEQKIEFKSVPLSS
                     NRTVPQFTFTRTPISLLTSYYDYMIGSYNGMPVQVIPDNVTGGGGYFLTYHARRTATG
                     IRRVFYNYLGADNYIYNSIEITNVTNNEGYPTVAVDPVSGKPFYAWHCNTDPDAQLEV
                     QLTSDAFLEGFCGLFNDYITLIDNPITVSGTTDNEFIWPTAVIGPSPFAGKRRIYIVA
                     KNSVTHTYGPSENPRIAWADFNGNDIENGYPLFWNYTSIPELDQWNIDIQWRRPFFAI
                     TCDNIGNLYYAGFHFAKEANGSTDILEPDLDVFKCNNYGTGTWVHLSKWSNLPSWNPD
                     ISPTNNNGYFTDTNNIPYEDNELFWKVMSENSGHLNANVDDLGRIHYPALWGLNNADG
                     YFYPAMQYPKEVIFDPSQPIDSQFRINEIWPQKNPANTFDEYYQPWDNEPPWGQAEYE
                     QDTGLYPRIYDSWDFPYWDQTAHNNAMFFHYNNMKITEGNGHGMLAVVWQNCRRAKLY
                     NADGDTDYSPWANTPEIWISVSSDNGDHWSEPIKLNNIEIPEFAGLKPMWVYPANKVK
                     YVGMQGNQKIGKLGIMFYNDFTWGAYVISPGVGSTNDGGQVMFTELQIVFPPEGQSQD
                     PFGQPIILSGSMSVMAGVHINGQMASVGDVVAAYVNVNGSPQLRGKETVQINDNIAGC
                     LLQVYTETNGETVYFKVWDASTNQVYNVTETLSSQVNGVIGSWPDNLFWLNATLTQQQ
                     NIPLNTGWNMVSLNVHPNSMAITDIFANIMANIVMIKSPDGVFIPGNPYNTLTQLADG
                     KGIFVKVNNPCVLSVTGMPIDITAPIYLNAGWSLIGYILPIPLNISSALISLTGNYIQ
                     VKGVEGVFEPNNPFSTLSVLSPGRAYWIKLLNPGVLFYAYPLKEEQSPQNILSCTKWG
                     TPMQKTNSQAVLCSLGSFASEGDQLAALVQGELRGLAPLIKHNSILGTYINIFTDSPG
                     ETIEFKLIRKNSGEIIDLQPNLSSEPGETTGDYSNGIFYTLTQAPNQTPEIVTELVSA
                     YPNPFSVSTSITLNVGKEKQPVQIDIYNLRGQKITTLLQDKLEFGQHNIVWNGTDENG
                     RRVASGVYLCKMQSENKTQSIKLMILK"
     gene            5401..6957
                     /locus_tag="PLG69_05575"
     CDS             5401..6957
                     /locus_tag="PLG69_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPI43015.1"
                     /translation="MIRKIIPLLLLTLFAGAALAIDPWGEPEILPGSMTVMAQVSISG
                     SPAVNNDVLAAFVNVNGTLQLRGKAFIQVFGGVSGCLIQIYTENNGEIITFKVWDESI
                     QTVFNANQTLVSEVNSIVGSYPDNMYQIEAGQTTVTDPWLEPVILTSSMTVMAKIGIS
                     GVPATNEDLLAAFVTVDGIEQLRGKGNISVINGIAGCLLQIYTEVNGETIYFKVWDYS
                     AQQIVNAFPTLNSEVNGIVGSWPNNLFHIYAGDVQTVATPAFLPLGGTYQTAQNVTLT
                     CATSGAQIRYTLNGTDPTETSALYTNPIHLNLNSTTEIRAKAFKEYWIPSNIVSNTYI
                     ITGTVPAPSFNPPAGSYLNAIDVIISCALEETVIRYTLDETEPNENSMLFTAPIHINQ
                     DTMLKAKAYKTDWLPSNVVSAIYQISSANEDNLNAPLVTGIQNVYPNPFTGCTTIEIA
                     IKEYPQNYLLKIYNIKGEQVRQFNGSAKGIITQNWDGTDNKGNKLAAGVYLLYFQVGS
                     NHYTRKLILQ"
     gene            7656..7838
                     /locus_tag="PLG69_05580"
     CDS             7656..7838
                     /locus_tag="PLG69_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPI43016.1"
                     /translation="MKIDKNLDIWKKGRQLATNVYELAKELPEEEKFVLSHEITQTAI
                     LMISEIPNSSTHALQN"
     gene            7854..>9174
                     /gene="der"
                     /locus_tag="PLG69_05585"
     CDS             7854..>9174
                     /gene="der"
                     /locus_tag="PLG69_05585"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPI43017.1"
                     /translation="MRKYIVSIVGRPNVGKSTLFNRLCRKRSAIVDFEAGITRDRKYE
                     DVEWNGKIFKLVDTGGIVFNSIETIDKMVLHQVMLAIDESDLIIFMVDAQTGTTDIDK
                     EIAKILYPHKDKVMLVANKADNEKYEWEVYDFLQLGFGDVIPISAAQGRNTGDFLDAV
                     IERIPGTQSIFQLEEKSSATRIAVVGKPNVGKSSIVNLLLGNPKLIVTEIPGTTRDAI
                     DSPFRYHNKDYVLIDTAGLRRKTRVNYGVEYFSTLRTIEAIDRCDIVVLVLTADEELS
                     VQDIKIASYAKRKMKEIMVVYNKWDLIEKETNTINKYLKELHYQMPFLQFAPVLFISA
                     KTTQRIHRIMETVAKIEEESEKRISTAELNRFMEKVIMHRPPTHPSGRHIKIYYITQA
                     AVKPPTFIFFCNTPSLITENYRRYLHNQLREMFKFEGVSIKLIFKGRK"
BASE COUNT         2888 a         1681 c         1803 g         2802 t
ORIGIN      
        1 gtggtcctca ttcttattat gtaactgcta tgtatggaac tgttgaatcc ggtgcctcta
       61 atacagtaaa tactttcatt attcctcctc agcattatga gcttagttat gatgatggaa
      121 cagcagaaca aggatttagt gttggagtta ccagacagat ggcagttaaa tttaattatt
      181 ttggtgaatt tgatttacaa catattaaag tatatgtgca cactcttgga acagcaagca
      241 ttattattcg tattcatgat gcttcagggg taaatggtat gcctggtgat ttatattgtc
      301 tataccatta tcccgcaact tctttagttg ttggctggaa ctttattact ttgcctacac
      361 cggttcattt taacaatcaa tctttctatg tcggtattct ggaaacaact ggcgcttctc
      421 aaattggtct ggataccagt tcttttggcc atagctataa aaatattagc ggtagttggg
      481 aattgattgg aactggagaa ataatgcttc gggcaattat ctcatccgat caaatagacc
      541 cttttgggga gccgattacc ttatctacaa gtatgacggt ggtctgtgga gcaacaatca
      601 atggtgttca agcttcccaa ggcgatgtat tagctgccta tgttaatgta aatggaagtc
      661 ctcagctgag aggtaaacaa acggtgatgg tgcaaaacaa tattgccggc tgtattcttc
      721 aggtctatac ggaagtcaat ggtgaaaatg taaccttcaa gatttgggat cagagtgcaa
      781 atcaggtcta ttcaacctcc aatgttctac ctacaacagt gaatggaact atcggtagtt
      841 ggcctgataa cttgtatcag ataaatggta taggatatac tacgcaaaat attcctttag
      901 tcagtggctg gaatctggtc tccttaaatg tttctcctta tgaccatagt ccaggttctt
      961 tatttgctcc tttgatcggt caggttcagc aagttaaggg aacagaaggg atatatattc
     1021 ctaataatcc ttattctact ctaactgctc taacggatgg caaggcatat tctattctga
     1081 tgagtagtcc tgttacctgg agtgtgacag gaattcaaat tccggcttct accccaattc
     1141 ctttagtcaa cggttggaat atggctgcct ttttaccttc caactcgtta tctgttacta
     1201 ctgcgatgtc ttctatttct tcctggttga tccagcttaa aggaaccgat ggggtttata
     1261 ttccttctaa tccttattcc acattaaata ctatgtatcc gggtaagggc tactggataa
     1321 atataaatgg agctcataac ctgatttacc ctgggaaaat tgcctctgct gagggaattc
     1381 gttctgttac tatctctcct gctaaagagc aggaagcagt taaaatgctt tccaatagct
     1441 ttactcttct ggcaaaatgt cctgaagcag aagtaggtga ttatttactt gccagagtga
     1501 atggagaatt gcggggtaaa gagttgctga ttgctccgga aggttttttg gccgctctga
     1561 ttcaggtcta tagtgatatt atgggagaag aagtgagatt ttctctgtgg aagccgtcgg
     1621 ggatggaagt ggctttgcaa aatactataa gtagtgagcc gggagctatt ataggtaact
     1681 atcctaactt catagaactt tatatagagc ccttaactac agaagaacat attgctattc
     1741 ttacttgtct acaaggtgtt tatcctaatc ccttcaatcc ttctactact atctcctttt
     1801 ctgtaagtga agataatagc cctgtgaaga taactattta taatataaaa ggtcaaaagg
     1861 ttcgctgtct ggtgcaggaa aatttccctt gcggtgagta taaaattatc tgggacggtt
     1921 tgaatgacca cggaactacc gtttcttccg gggtctattt tgctgaaatg cgtacacccg
     1981 gatatcgtaa acaaattaaa ttattactta ctaaatgaag atttttccag gaggaaaaat
     2041 gaaaatccga attttaatcc tatccgtatg tatcttaatg ctttcctctg tgctactggc
     2101 acaggagatg gttcctcttt ccaatttgaa agagcagaaa attgaattta aaagtgttcc
     2161 gctatccagc aacaggacag ttccccaatt tactttcacc cggactccta tttctttact
     2221 tacttcttat tacgactata tgataggaag ttacaacgga atgccagttc aggtgattcc
     2281 ggataatgtt accggtgggg gcggttattt tttaacttat catgccagga gaactgcaac
     2341 cggaatccga agagtatttt ataattatct tggtgctgat aattatattt ataacagtat
     2401 tgaaattaca aatgtaacaa ataatgaagg atatcctact gtagcggtag accctgtttc
     2461 cggaaaacct ttctatgcct ggcactgtaa tactgatccc gatgcacaac tggaagttca
     2521 gcttacttct gatgcttttc ttgaaggttt ttgcggactg tttaacgact atataacgct
     2581 tattgataat cctataacgg tctcaggtac aactgacaac gaatttatat ggcccacagc
     2641 agttatcgga ccttctccct ttgctggcaa aagacgaatt tatatagttg ccaaaaactc
     2701 tgtaactcac acttacggtc cctccgaaaa tcccagaatt gcctgggcag attttaacgg
     2761 aaatgacatt gagaatggtt atcctctttt ttggaattat acttccattc cggaattgga
     2821 ccaatggaat atagatattc aatggcgtcg tccttttttt gccattacct gtgataatat
     2881 aggaaatctc tattatgcag gttttcattt tgctaaggaa gctaatggtt caactgatat
     2941 ccttgagccg gatttagatg tttttaaatg taataattac ggaaccggaa cctgggttca
     3001 tcttagtaag tggagcaatt taccttcctg gaatccggat atcagtccta caaataataa
     3061 tggttatttt acagatacaa ataatattcc ttatgaagat aacgagcttt tttggaaagt
     3121 aatgtctgaa aattccggtc atctgaatgc caatgtggat gaccttggta gaattcatta
     3181 tcctgctctt tggggattga ataatgctga tggctatttt tatccggcta tgcaatatcc
     3241 caaagaagtg atctttgatc cttctcaacc aatagattcg cagttcagaa taaatgaaat
     3301 ctggcctcaa aaaaacccgg ctaacacttt tgatgaatat taccagccat gggataatga
     3361 accaccttgg ggacaagcag aatatgaaca agatactgga ttgtatccca gaatttacga
     3421 tagctgggat ttcccctatt gggatcagac cgcacataat aatgctatgt tcttccatta
     3481 caataatatg aaaataactg aaggcaacgg acacggaatg ttagcggttg tctggcaaaa
     3541 ttgcaggaga gctaaactat ataatgcaga tggtgatacc gattattccc cttgggcaaa
     3601 tacgcctgaa atctggattt ccgtttcttc cgataatgga gatcactgga gtgagcccat
     3661 aaaactgaat aatattgaaa tacctgaatt tgccggatta aaaccaatgt gggtttatcc
     3721 tgccaacaaa gtaaaatatg taggaatgca gggtaaccaa aaaataggta aattgggcat
     3781 aatgttttac aacgatttta cttggggtgc ttatgttatc agccctggag ttggtagtac
     3841 aaatgatggc ggacaagtga tgttcaccga attgcaaatt gtttttcctc ctgaaggaca
     3901 atctcaagac ccttttggac agccaattat tctcagcggg agtatgagtg ttatggctgg
     3961 agtgcatatt aacggacaaa tggcatcggt aggagatgta gtagctgcct atgttaatgt
     4021 aaatggaagt ccccaattac ggggcaagga aaccgttcag attaatgata atattgccgg
     4081 ttgcttgcta caggtttaca cagaaaccaa tggagaaacg gtgtatttca aggtctggga
     4141 tgccagtacc aatcaggtct ataatgttac agaaaccctt tccagtcaag taaatggggt
     4201 aattggtagc tggcctgata atcttttctg gctaaatgca actttaactc agcagcaaaa
     4261 tatccctcta aatactggtt ggaatatggt ttcgctcaat gttcatccaa acagtatggc
     4321 aattacagat atctttgcca atataatggc aaacattgta atgatcaagt caccggatgg
     4381 agtatttatt cctggcaatc cttataatac tttaacccag ttagcggatg gcaaaggtat
     4441 atttgtaaaa gtaaacaatc cttgtgtgtt atccgtaacc ggaatgccga tagatataac
     4501 agctcctatc tatttgaatg cgggttggag tttaattggt tatattctcc ctattccttt
     4561 gaatatcagt tctgctttga taagtttaac cggcaattat attcaggtaa aaggagtaga
     4621 aggcgtattt gagccgaata atcccttcag tactctttct gttttaagtc ccggaagagc
     4681 atactggatt aaattgctaa atccaggtgt tctattctat gcttatcctc ttaaagaaga
     4741 acaaagtccg caaaatattc tttcctgtac caaatgggga acaccgatgc aaaagacaaa
     4801 cagtcaagcg gtcttatgtt ctttaggtag ttttgcttct gaaggagatc aactggcagc
     4861 tttagttcag ggagaattga gaggattagc tcctttaata aaacacaata gcattttagg
     4921 aacttatatc aatatcttta ccgatagtcc tggagaaaca attgaattca agcttatccg
     4981 gaaaaacagt ggagaaatta tagatttaca gcccaattta agcagtgaac cgggagagac
     5041 aacaggtgac tatagtaatg gaatatttta tactttaacc caagctccga atcaaacacc
     5101 ggaaattgtt acagaacttg tttctgccta tcccaatcct ttttcagttt ccacttccat
     5161 taccctcaat gtgggcaaag aaaaacagcc cgttcaaata gatatttaca atttgcgggg
     5221 gcagaaaatt actaccttac tgcaggataa actggaattc ggacagcata atattgtttg
     5281 gaacggaact gatgaaaatg gcagaagagt ggcttccggg gtctatttat gcaaaatgca
     5341 atcagaaaat aaaactcaaa gcatcaaact aatgatcctg aaatagaaaa aggaggaata
     5401 atgattcgca aaattattcc ccttctgctt cttactttat tcgcaggagc agctttagct
     5461 attgaccctt ggggtgaacc ggaaatttta cccggcagta tgaccgttat ggctcaggta
     5521 agtatatctg gaagccctgc agttaataat gatgttttag ctgcctttgt aaatgtaaat
     5581 gggacacttc aattacgggg caaagcattt attcaggtgt ttggaggtgt ttccggatgc
     5641 cttattcaga tttacactga aaataatggc gaaataataa ccttcaaagt ttgggatgaa
     5701 agtatccaga cagttttcaa cgctaatcaa accttggttt cagaagttaa tagtatagta
     5761 ggaagttacc ctgataatat gtatcaaata gaagcaggac aaacaaccgt aaccgatccc
     5821 tggctggaac ctgtaattct gacaagtagt atgacggtta tggctaaaat tggcatttcc
     5881 ggagttcctg ccactaacga agatcttctg gctgcttttg taactgttga tggtatagaa
     5941 caattacggg gaaaaggtaa tatctctgtt atcaatggca ttgccggttg ccttctgcaa
     6001 atatacacag aagttaatgg tgaaactatc tattttaagg tttgggatta tagtgctcaa
     6061 caaattgtta atgcttttcc tactctaaac agtgaagtaa acggaatagt cggttcctgg
     6121 cccaataatc tgttccatat ttatgcagga gatgtgcaaa cggttgctac acctgcattt
     6181 ttacccttgg gaggaaccta tcaaactgct caaaatgtaa ctctcacttg tgcaacttcc
     6241 ggagctcaaa ttcgttacac tttaaatgga acggacccta cagaaacttc tgctctctat
     6301 accaatccta ttcatctgaa tttgaacagc actaccgaaa ttagagctaa ggcatttaaa
     6361 gagtattgga tacccagtaa tattgtaagt aatacctata tcattactgg aacagttcca
     6421 gctccttctt ttaatcctcc cgcaggttca tatctcaatg ccatagatgt tattatcagt
     6481 tgtgctcttg aggaaacagt tattcgttat actctggatg aaacagaacc caatgagaat
     6541 tcaatgcttt ttactgctcc aatacatata aaccaggata caatgcttaa agctaaggca
     6601 tataaaactg actggctacc cagtaatgtt gtttccgcaa tttatcaaat ttcttccgct
     6661 aacgaagata acttgaatgc tcctctggtt accggtattc aaaatgttta tcccaatcct
     6721 ttcaccggtt gcactacaat tgaaattgct atcaaagaat atcctcaaaa ttatctcctg
     6781 aaaatttaca atattaaagg agaacaagtg cgtcagttta atggcagtgc taaaggaatc
     6841 atcactcaga actgggatgg aactgataac aaaggcaata aattagccgc gggagtttat
     6901 ctcctttatt ttcaagttgg ttccaaccat tatacccgca agctcatctt acaataaata
     6961 tctattcaat tgacggggac tgaaaaattt ggtccccgtc aattccttct ctcggaaaaa
     7021 cgatatcctc gtctttttaa aatcagaagt cgttactgct gaaataactt ttacgctatc
     7081 tgtgaacttt tatggcagta atgactaatg acatcgttaa tttattattt tagcgttggt
     7141 ttcaataagt tattgttcaa ggtcgtgatg agttatatac accgttaaag gtatctttaa
     7201 gaataaagta tcgttggcgc aaatgactcc tgacttttaa agacatcaat cttctgggtt
     7261 caattttaca ctttaaaaat caccgttctt accctcatta ctttatcatt gctcacccat
     7321 tactaatgcc atccgtaatg ggtaagcaat gagtaagcaa tgcttttaag gaagaagtag
     7381 taactatctt ttaatcaggt agttaccaat atagtatgaa acattatagg aataatggat
     7441 tcatttagaa gaaaatagaa cccagataac tgaatagagg aattgaacag gattttttta
     7501 tcctaaatcc atattcataa atcataaata acagaaaaaa ttccgtagga atggcagcaa
     7561 cagatgaggg ttgacaaaat gaagcactac agaaattggt tttattattc aaagtaaagg
     7621 aaatataaaa gtcaattaag cacaggagaa gagaaatgaa aatagataaa aaccttgata
     7681 tatggaaaaa aggaaggcaa ttagcgacaa atgtttatga attagctaaa gaactgcctg
     7741 aagaagaaaa attcgtttta tctcatgaaa ttacccaaac tgctatccta atgatttcag
     7801 aaattccaaa ctccagcact catgcactcc agaactaaaa aactaaatta acaatgagaa
     7861 aatacatcgt ttccatcgtg ggtcgtccga atgttggaaa atccacactt ttcaatcgct
     7921 tatgccgtaa gcgttctgct atagttgatt ttgaagcggg aattacaaga gatcgtaaat
     7981 atgaagatgt ggaatggaat gggaaaatat ttaaactggt agatacagga ggaattgttt
     8041 ttaacagcat agaaaccata gacaaaatgg ttctgcacca ggtgatgtta gctattgacg
     8101 aaagcgattt aattatattt atggtagatg ctcaaaccgg cacaacggat attgacaagg
     8161 aaattgcaaa aatcctttat ccgcataaag ataaggttat gctggttgcc aataaagctg
     8221 ataatgaaaa atacgaatgg gaggtttatg atttcctgca gcttggtttt ggcgatgtta
     8281 ttcctatttc cgctgcccaa ggacgcaata cgggtgattt tttagatgcc gtaatagaac
     8341 gcattccggg aacacaatcc atttttcaac tggaagaaaa gtcctctgct acgcgcattg
     8401 ctgttgtcgg aaaacccaat gtaggcaaaa gttctattgt aaatctgctt ttaggcaatc
     8461 caaaattgat tgtaactgaa attcctggca caaccagaga tgctatagat agtcctttcc
     8521 gctaccataa taaggattat gttttaattg atacagcggg actaagacga aaaaccagag
     8581 ttaattatgg agtggaatat ttttctacct taagaacaat tgaagccata gacagatgtg
     8641 atattgtggt tttagtgctc acggcagatg aagaacttag cgttcaagat attaaaattg
     8701 ccagctatgc gaaacgcaaa atgaaagaaa ttatggtggt ctataataaa tgggacttaa
     8761 tagaaaaaga aaccaatact atcaataagt atctgaagga attacattac caaatgccct
     8821 ttctgcaatt tgcccccgtg ctttttattt ctgcaaaaac aactcagcgc atccatagga
     8881 taatggaaac ggtagctaaa attgaagaag aaagtgaaaa gcggatttct acagcggaac
     8941 ttaaccgttt tatggaaaaa gttattatgc acagacctcc tacccatccc agtggaaggc
     9001 atataaaaat atattacata acacaagcag cggttaaacc gcctactttt atcttctttt
     9061 gtaatactcc ttctctgata acggaaaatt atcgccgtta tctgcataat caattgcggg
     9121 aaatgttcaa atttgaagga gttagtatca aactcatttt taagggacgc aaaa
//
LOCUS       DAOPFL010000069         8669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_10205_length_8669_cov_90.443812, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000069 DAOPFL010000000
VERSION     DAOPFL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10205_length_8669_cov_90.443812"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLG69_05590"
     CDS             <1..216
                     /locus_tag="PLG69_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPI43018.1"
                     /translation="VTDIAMPDMDGYELFWRIKDYNEKIPVIMMTGFGYDPNHVLVRA
                     KVDGLKDVLYKPFETEKLVELIESHLQ"
     gene            217..1269
                     /gene="lpxK"
                     /locus_tag="PLG69_05595"
     CDS             217..1269
                     /gene="lpxK"
                     /locus_tag="PLG69_05595"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPI43019.1"
                     /translation="MDANKLIQNHLLKRSPLSYTLYPAGVIYGFLQKIRRTYYQRNEY
                     KPPCKIISIGNIVSGGSGKTPLTISLAKLLIKEGKKVAVSHRGYKGKFEYNPHLIADK
                     TGILYDVQHTGDEAYMIASSLPQIPVVVGRKRKEAIQLLLQNYPDLEIIILDDALQHY
                     YVHRDLDIISFDASLGLGNGFVIPAGYLREPISNIPSQSIAVINHKNEEQTQIPWLEK
                     IIRKGMPVFHCFSSPSCFKDAAGNTFPFSYIEGKRIVLTSAIANPASFEQSVCSLGLS
                     FMQHYAFKDHYAFSNTFLPKKLLELNPDFILCTQKDIMKLAKYESLKNRLLALELEYS
                     MENKEGFVNQVLKFVR"
     gene            complement(1705..1938)
                     /locus_tag="PLG69_05600"
     CDS             complement(1705..1938)
                     /locus_tag="PLG69_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HPI43020.1"
                     /translation="MNNKKLKILNPIMFVVILIAIIAIITYKISEAFNYDAYGFKEIH
                     EICGFLFVILAIFHIIYNWQWIRTQIFKKKPKK"
     gene            complement(2149..2853)
                     /locus_tag="PLG69_05605"
     CDS             complement(2149..2853)
                     /locus_tag="PLG69_05605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPI43021.1"
                     /translation="MPSIYLSELSSEKKIITVEGEEYHHLINVKRTGIGESVKLNSGA
                     GLMAEGKLIKVNKKSAEIEIDKIDKVPYPERPFAIAFALLKNRRDDWLIEKCTELGVT
                     AFYPMLTDYSVRTPSQNTITRFNLIALAAIKQCDNPWMPKINEPLPLEETVNKIFSEG
                     WIPVLCSERKPDKRITYLPKIIKPCFLIGPEGGFSEDEFTYFSEQNILSISICHLITR
                     AETAAIAVAAQYQAYF"
     gene            complement(2859..4016)
                     /gene="dnaJ"
                     /locus_tag="PLG69_05610"
     CDS             complement(2859..4016)
                     /gene="dnaJ"
                     /locus_tag="PLG69_05610"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPI43022.1"
                     /translation="MAKRDYYEILGVDRNADEAAIKKAYRKLAMQYHPDKNPNNKEAE
                     EKFKEASEAYEVLSDKEKRQIYDQYGHSGLENQFGGTGFSWEEFMHRSDLNDIFGDGL
                     GSIFETIFGGGFGGRNQRSSGNSNRGEDLQIELSLSLQEIANGVEKTIKIGIKEPCDK
                     CGGSGSAEGKTETCPNCKGTGQIRQIRQSLFGRMQTVSECPTCNGEGRIIKNKCSKCY
                     GEGRVGTVKEIPINIPPGVEDGQYIRFRGQGNAGPRGGSRGDLLVLIHQKKDDLFERE
                     GNNIILEYPITVSQAVLGDEIIVPTLTGSVKMKVPPGTQSGRLFRLKGQGIQGHNSYS
                     RGDQLVRTIVVIPTKISREEAELYNRLREFDRKRDLKPGKSFAAKLKSYFS"
     gene            complement(4182..6140)
                     /gene="dnaK"
                     /locus_tag="PLG69_05615"
     CDS             complement(4182..6140)
                     /gene="dnaK"
                     /locus_tag="PLG69_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPI43023.1"
                     /translation="MGKIIGIDLGTTNSCVAVVEGGKPVVITNAEGSRTTPSVVGFTK
                     DGQRLVGQLAKRQAITNPQNTVFSIKRFMGRSYDEVTQEIKQVPFKVVRGLKNQAVVH
                     IDVENKDYAPQEISAMILTKMKETAEAYLGQKVTEAVITVPAYFNDDQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMENKKEQKVAVYDLGGGTFDISILDISEGVVEVLSTNG
                     DTHLGGDDFDKRVIDWILEQFKKQEGMDLSRDPMAMQRLREAAEKAKIELSGTLTTNI
                     NLPFITADATGPKHLNLDLTRAEFNRLTSDLVERTLGPCKRALDDAKLKPSDIQEVLL
                     VGGSTRIPAVQEAVEKFFGKSPNRSLNPDEVVAIGASIQGAILSGDEKVSDVLLLDVT
                     PLSLGIETLGGVMTKLIERNTTIPTKKSEIFSTAADNQTAVTIHVLQGERPMAADNKS
                     LGRFDLVGIPAAPRGVPQIEVTFDIDANGILHVSAKDKGTGKEQSIRIDRAGSISKED
                     IDRMIKDAELHSEEDKKRQEFVVAKNELDSLIFSTEKSLNEYGDKLSSSERSELEEEI
                     KNAKEQMERATEASELNNIKENLAKKAQHLGEVIYAHMQQQQSAGGEGYSQDASGFNS
                     GGYEQGPQEEHPKQEGPIDADFEVVDDK"
     gene            complement(6141..6698)
                     /gene="grpE"
                     /locus_tag="PLG69_05620"
     CDS             complement(6141..6698)
                     /gene="grpE"
                     /locus_tag="PLG69_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPI43024.1"
                     /translation="MNKKIPKEPELEKEELITTEVSEEPKAKKIEELEKEVAEWKDKY
                     LRSMAEFENFRKRTISEKAEWIRLATQKFALEICDVLDNFERAIQQATEEEKSTPFGK
                     GVLMIEQQLRKALEKEGVKRIEALGEPFNPEFHEALAHIPSDQEENTVTAIIQNGYIM
                     NDKVLRPARVAVSNGSKINNEFQEE"
     gene            complement(6695..7747)
                     /gene="hrcA"
                     /locus_tag="PLG69_05625"
     CDS             complement(6695..7747)
                     /gene="hrcA"
                     /locus_tag="PLG69_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPI43025.1"
                     /translation="MKKNQLRLNETLKALVDEYIMSCEPVSSRILNEKYLRNVSSATL
                     RLDLLKLEKMNLISQPHTSAGRIPTIKGYREYLRLIEPEIEKIRYEGMDLLREILVHN
                     YKDTPRALYYIMENLAKETDQLSFVAEPEVSSGYLAKLDVFSIGENKLIFVMSLDSGL
                     DKTVILKCDNELTEAQLKKLVRYLNDELAGLQIYDIANRVLVEMQENADNGMISWFLA
                     ELHKAFMEISDFFIHYDGSINFLEQKEFNDKENILKFMSLLQRQDFLLNTMRKHQSDK
                     EVNIVLGEDLAKGSWLDFALIYAKYEVFGIPGYLGVLAPVRTEYRKLIPLIRDVANTI
                     TNTTRRGMVVPQEKKE"
     gene            7957..>8669
                     /locus_tag="PLG69_05630"
     CDS             7957..>8669
                     /locus_tag="PLG69_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HPI43026.1"
                     /translation="MKEYSNLKQGLTTKEVEEKLRREGYNELPGSKGRNLGKLIWDII
                     SEPMFILLVACAVIYFFLGDLQEALVLTASVVIVMSIELIQERKTEKALEALKNLSSP
                     RALVIRNGEQIRIPGREVVTDDLIILAEGDRIPADAYILENDLLSVDESLLTGESVPV
                     RKRIWDKKERPSTPGGDDLPFVYCGTLVVSGRGIAQVFATGERTELGKIGKSLETITE
                     EETRLSKETGRLVKYFAIFA"
BASE COUNT         2497 a         1788 c         1476 g         2908 t
ORIGIN      
        1 gttacagata tcgctatgcc cgatatggat ggttatgaat tgttttggag aattaaggac
       61 tataacgaaa aaattccggt gattatgatg acgggttttg gctatgaccc caatcatgtg
      121 ctcgttcgcg ctaaagtaga cggtttgaag gatgtccttt ataaaccctt tgaaacggaa
      181 aaactggtgg aactaattga atcccacttg caataaatgg atgctaacaa gttaatccaa
      241 aatcaccttc taaaacgctc tcccctatcc tataccctat atcccgcagg tgtaatttac
      301 ggtttcctac aaaaaatacg cagaacttat tatcaaagaa atgaatataa gccaccttgc
      361 aaaataatta gtatcggtaa tatcgttagc ggcggttcgg gaaaaacacc tctaactatt
      421 tctctggcaa agcttttgat aaaggaaggg aaaaaagttg cagtttccca tcgcggttat
      481 aaaggcaagt ttgaatataa ccctcatcta attgcggata aaacaggcat tctttatgat
      541 gttcaacaca caggtgatga agcatatatg atagcttctt ccctgcccca aattcctgta
      601 gtggttggca gaaaacgaaa agaagcaatt cagctcttat tgcaaaacta tccggatctt
      661 gagataatta ttttggatga tgccttacaa cactattatg tgcatagaga cctggatatt
      721 atcagttttg atgcttctct tggacttggc aatggctttg tgattcctgc cggctattta
      781 agagagccaa tttccaatat tccttcccaa agcatagctg ttattaacca taaaaatgaa
      841 gaacaaacgc aaattccctg gctggaaaaa attatccgca aaggaatgcc tgtatttcat
      901 tgttttagct ctccctcctg ttttaaagat gctgccggta acacctttcc tttctcttat
      961 atagaaggaa aaagaatagt cctcacctca gccattgcca atcctgcttc ctttgaacag
     1021 agtgtttgct ctttgggctt atcttttatg caacactatg cctttaaaga ccattacgct
     1081 ttttccaaca cttttctgcc gaaaaaactg ctggagctaa atccggactt tattctctgc
     1141 acccaaaaag atattatgaa actggcaaaa tatgagtccc tgaaaaaccg tttgctggct
     1201 ctggaactgg aatatagtat ggaaaataag gaaggatttg ttaaccaggt actgaagttt
     1261 gttagatagt gagatgaaat gatagttata tagaaaataa aggaaaaaga gaagtaacct
     1321 gaaaaatcca tatttattta aaatcatagc agggttggta gattctaatt attggtttta
     1381 aactcggtga tcagaaaaat tcatagagct aaatactatg tagtctgagg acacttttcc
     1441 tccgtatatg aaagtcaatt gttaggttag gagccattta cgcttttact attttgtcct
     1501 taccaattac acttttaaca caagggaagc ataacggcta taatatgcta taaatataaa
     1561 tagtttcctc cgttatgaat cctcactgct aaatcatata tcggtagtga aataaatctt
     1621 gatggcattg ataactccta aattttccgg aaaacgatgc ccctctcgtg ttttccatta
     1681 atttagatta aacaaaaatt atcattattt tttcggcttt tttttaaaaa tctgagttct
     1741 tatccactgc cagttgtaaa ttatatgaaa tattgccaaa ataacaaaca aaaatccaca
     1801 aatttcatgg atttctttaa aaccataagc atcataatta aaagcttctg agattttgta
     1861 agtaattatt gctataatgg caataagaat taccacgaac ataattggat tcaatatctt
     1921 cagcttcttg ttattcatat cttactcctt tttataatat tataaaccat gtttatgctc
     1981 tggcaaattg attttattaa gtaatatata gagaaatagt tagataatta gatagtaaaa
     2041 tagtgaaatg gtgagatgct aaaatggtaa agatgattgt agtatatgat ctaaaatcca
     2101 agttatcaat taccattcta tcacttatcc attgtttttt ttctttgttt aaaaataagc
     2161 ttgatattgt gcagcaacag ctattgcagc agtttcagcc cgggtaatta gatggcaaat
     2221 tgatatagaa agaatatttt gttcactaaa ataagtaaat tcatcttcac taaacccgcc
     2281 ttcaggacca attagaaaac agggtttaat aatcttcggt aagtaggtaa ttcttttatc
     2341 aggttttcgt tcagagcata aaaccggaat ccacccctcg gaaaatatct tatttactgt
     2401 ttcctccagc ggcaaaggtt cattaatttt cggcatccaa ggattatcac attgtttaat
     2461 tgctgctaat gctataagat taaaacgcgt tatagtattt tgggaaggtg ttcgcacact
     2521 atagtcagtt aacataggat agaaggcagt aactcctaat tctgtacact tttctatcag
     2581 ccaatcatcg cgtcggtttt ttaataaggc aaaggcaatg gcgaaaggtc tttccggata
     2641 aggaacttta tctattttat caatttcaat ttcagcggat tttttattaa ccttaattag
     2701 tttgccctct gccattaaac cggcaccact attcagtttg actgattctc cgattcctgt
     2761 tcgcttaaca ttaatcagat gatgatattc ctctccttct accgttataa tcttcttttc
     2821 cgagcttaat tctgataaat atatagatgg catttttctt agctgaagta actctttaat
     2881 ttggcagcaa agcttttccc cggtttcagg tctcttttcc ggtcaaattc tcttaagcga
     2941 ttatagagtt ctgcttcttc ccgggagatt tttgtaggga taactacaat cgttcttact
     3001 aattgatcac ctctggaata actgttatga ccttgaattc cttgtccctt taagcggaat
     3061 aatctgccac tttgagttcc gggtggaact ttcattttaa ctgaaccggt taaagtaggc
     3121 acaataattt catcacctaa cacagcttga gacacagtaa tgggatattc cagaataatg
     3181 ttattccctt cgcgttcaaa aagatcatcc tttttttgat gaataagaac aagaagatct
     3241 cctctgcttc ctcctcgggg tcctgcattg ccctgtcccc tgaaccgaat atactgtcca
     3301 tcttcaactc ctggaggaat gttaatcggt atttccttaa cagttccaac acgtccttca
     3361 ccataacatt tactgcattt atttttaatt atcctgcctt caccattaca ggtagggcat
     3421 tctgataccg tttgcatcct tccaaaaagt gactgccgaa tttgacgaat ttgtcctgtt
     3481 cctttacagt tagggcaggt ctctgttttc ccttctgcac ttccgcttcc tccacattta
     3541 tcacagggtt ctttaatgcc tattttaatg gtcttttcta caccattagc aatttcctga
     3601 agtgaaagtg atagttcaat ttgtaggtct tcccctctat tggaattgcc tgaactgcgt
     3661 tgatttcttc ctccaaaacc acctccgaag attgtttcaa aaatgctgcc cagtccatct
     3721 ccaaaaatat cattcagatc gctacggtgc ataaattctt cccaagagaa tccggttcct
     3781 ccaaattgat tttctaatcc ggaatgtccg tattgatcgt atatttgtct tttttcttta
     3841 tccgataaaa cttcataagc ttcacttgct tctttgaatt tctcttcggc ttctttatta
     3901 ttcgggtttt tatccggatg atactgcatt gccagtttac ggtatgcctt ttttatggct
     3961 gcttcatcgg catttctatc tacacccaga atttcgtaat aatcgcgttt tgccatctat
     4021 atgttacctc tcacagattt cactgatttc acagatataa agatataaac tataataacc
     4081 tgttttcttt cctttgccga tttatggatt agagggatta acacggaatt atttttcatc
     4141 cgtgttaatc cgctaaaatc cgtttaatca gttttcttta tttatttatc gtcaacaact
     4201 tcaaagtcgg catctatagg accttcctgc ttggggtgtt cttcctgagg tccctgttca
     4261 taacctccgg aattaaaacc gcttgcatcc tggctatatc cttcaccgcc tgcactttgt
     4321 tgctgttgca tatgtgcata gataacctca cctaaatgtt gtgctttttt ggcaagattt
     4381 tcttttatgt tgtttagttc tgatgcctcg gttgctcttt ccatttgctc tttggcattc
     4441 tttatttcct cttccagttc gcttctttca ctgctggaaa gtttatctcc atattcattc
     4501 aaactctttt ccgtgctgaa gattaaggaa tccagttcat tcttagcgac aacgaattcc
     4561 tggcgttttt tatcttcttc ggaatgtaat tctgcatctt taatcattct gtctatatct
     4621 tccttactaa tgctcccagc acgatcaatt ctgatggatt gttcttttcc agttccttta
     4681 tctttagcag aaacatggag aataccattg gcatcaatat caaaggtaac ttcaatttga
     4741 ggaactcctc ttggagcagc aggaattccc accaggtcaa agcggcctaa ggatttatta
     4801 tctgcagcca taggacgttc accttgcaga acatgaatgg tgacagcggt ttgattatct
     4861 gctgccgtag agaaaatttc gctcttctta gtcggaatag tagtattgcg ctcaataagt
     4921 ttggtcatta ctccacccaa ggtctcaata cctaaagaaa gaggagttac atccaaaagc
     4981 agaacatcac ttactttctc gtcaccggaa agaatagctc cctgaatgga agcaccaata
     5041 gctactactt catccggatt caaactacga ttggggcttt taccaaagaa cttttccaca
     5101 gcttcctgaa ctgcaggaat acgagtactt ccacctacca aaagaacttc ctgaatatca
     5161 gaaggtttca gtttagcatc gtctaatgct cttttacagg gacctaaagt cctttccacc
     5221 aaatctgagg ttaaacgatt aaattcagct ctggttaaat ccagattaag atgtttaggt
     5281 cctgtagcat cagcagtaat aaaaggcagg tttatattgg tagtaagggt tccggaaagc
     5341 tcaattttgg ctttttcagc tgcttccctt aacctttgca tagccatagg gtcacgcgat
     5401 aagtccattc cttcctgttt tttgaactgt tccagaatcc aatcaattac ccgcttatca
     5461 aaatcatcgc cacctaaatg cgtatcaccg ttagtagaaa ggacttccac aacaccttct
     5521 gaaatatcca gaatgctgat atcaaaggtt ccgccaccca aatcataaac agctactttc
     5581 tgctctttct tattttccat tccataagct aaagcagcag ctgtgggttc gttgataatc
     5641 ctttttacat caagtcctgc aatgcgtccg gcatcttttg ttgcttgacg ttgatcgtca
     5701 ttgaaataag caggaacagt aatcacggct tccgtaactt tttgtcctaa atatgcctct
     5761 gccgtttctt tcatttttgt tagaatcata gcggaaattt cctgtggagc ataatcctta
     5821 ttttccacat caatatgaac aaccgcctga ttttttaagc cacgaacaac cttaaaaggc
     5881 acctgcttaa tttcttgtgt aacttcatca taacttcttc ccataaacct ttttatggaa
     5941 aagacagtat tttggggatt ggttattgcc tggcgttttg ccaattggcc aaccagacgt
     6001 tgaccatcct ttgtgaaacc aactactgaa ggagttgttc tgcttccttc cgcattggtt
     6061 ataactactg gtttgccgcc ttcaacaacg gcaacacagg agtttgttgt tccgagatca
     6121 attcctatta tttttcccat ttattcctcc tggaattcat tatttatttt tgaaccgttg
     6181 gaaactgcca ccctagcagg tcgcaacact ttatcgttca ttatgtaacc attttggatg
     6241 atggcagtaa cggtattttc ttcttgatca ctgggaatat gagcaagggc ttcatgaaac
     6301 tctggattga agggttctcc cagtgcttct attcttttta ccccttcttt ttccagggct
     6361 ttgcggagtt gctgttcaat catcagcacc ccttttccga agggagtgga tttttcttct
     6421 tcagttgctt gctggatagc cctttcaaaa ttatccagca catcacagat ttctaaagca
     6481 aatttctgtg tggctaatct tatccactcc gctttttcgg atattgttct tttgcggaag
     6541 ttctcaaatt cagccatact gcgtaggtat ttatctttcc attctgctac ttctttttcc
     6601 agttcctcta tctttttggc tttcggttct tctgaaactt ccgttgtaat tagttcttct
     6661 ttttctaatt caggttcttt gggtattttt ttattcattc ctttttctcc tggggcacga
     6721 ccattccgcg cctcgttgta tttgttatag tattagctac atccctgata agaggaatga
     6781 gcttacgata ttctgttctg acaggtgcta aaacacctaa atagccaggt atgccaaaga
     6841 cctcatattt ggcataaatt agagcaaaat ccaaccagct gcctttagct aaatcctcac
     6901 ccaaaactat attaacttct ttatcgcttt gatgtttgcg catcgtattt aagagaaaat
     6961 cctggcgttg cagtaaactc ataaacttca atatattctc tttatcgtta aattcctttt
     7021 gttccaaaaa attgatgctg ccatcataat gaataaagaa atcgcttatt tccataaacg
     7081 ctttatgcaa ttcagccaag aaccagctta tcataccatt atccgcattt tcttgcattt
     7141 ctactaaaac cctattagct atatcataaa tctgtaaacc agccaattca tcatttaaat
     7201 aacgcaccaa ttttttcagt tgagcttcag ttaactcatt atcgcatttc aaaataactg
     7261 ttttatccaa acccgaatcc aaactcatca caaatatcag cttgttttct cctatggaaa
     7321 atacatccaa ctttgccaaa taaccactgg aaacctcagg ttcagcaaca aaagaaagct
     7381 ggtctgtctc cttcgccaaa ttttccatta tataatacaa agctctgggc gtatctttat
     7441 agttatgcac taaaatctct ctcagcaagt ccattccttc atatctgatc ttttctattt
     7501 ctggctcaat caatcttaaa tattccctgt aacctttgat tgtaggaatc cttcctgccg
     7561 atgtatgcgg ctgggaaatc agattcatct tttccagttt caacaaatcc aaacgcaatg
     7621 tagctgagga tacattacgc agatacttct cgttcaatat cctggaagac accggttcac
     7681 aactcataat atattcatcc accagtgcct taagggtctc atttagtcgc agctgattct
     7741 ttttcattgt ctccaccatt tatattctta gcagtccaat atatgaactg ctaatctaaa
     7801 gacaatttaa tcaactttgg aaaattgtca atctaaaagt gagagtgcta aaaaaaattc
     7861 tgctgttccg atatttgtct cagctcaaaa tcggttttta cttgactaaa aacaatattg
     7921 cagttagcaa gattatattc tgtaaaagag gataagatga aggaatatag caacttaaaa
     7981 cagggattga ccacaaaaga agtggaagaa aaattaaggc gggaagggta taatgaactt
     8041 ccaggttcaa aagggcgtaa tttaggcaag ctgatttggg atattataag tgagccaatg
     8101 tttattctat tagttgcctg tgctgttatt tatttctttt taggggactt gcaggaagca
     8161 cttgttttaa cagcatccgt tgtcattgta atgtctatag aacttatcca ggaacggaaa
     8221 acggaaaaag cgcttgaagc attaaagaat ctatccagtc cgcgagcttt agtaattcgg
     8281 aatggtgagc aaatcagaat tccaggtaga gaagttgtca cagatgattt aattatatta
     8341 gctgaaggtg acaggattcc tgctgatgct tatatactgg aaaatgattt acttagtgtt
     8401 gatgaatcac ttttaacagg agaatcggtc ccggttagaa aacggatttg ggataaaaaa
     8461 gaaagacctt caactcctgg aggagatgat ttaccctttg tttattgcgg aacattagtt
     8521 gtgagtggca gaggtatagc tcaggttttt gccacaggag aaagaaccga actgggaaaa
     8581 ataggaaaat ccctggaaac cataacagaa gaggaaaccc ggttaagcaa agagacaggc
     8641 agattggtaa aatactttgc tatttttgc
//
LOCUS       DAOPFL010000070         8618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_10299_length_8618_cov_97.089571, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000070 DAOPFL010000000
VERSION     DAOPFL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10299_length_8618_cov_97.089571"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..2369)
                     /locus_tag="PLG69_05635"
     CDS             complement(306..2369)
                     /locus_tag="PLG69_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPI43027.1"
                     /translation="MVIEEYDVHNLDCPDCGAKIEAEINNLEEVESANLDFINKHLTI
                     EYIEKVDNPLKRLNAIASKIDPNVQFTLAGLEQERTEKKPWFIIVAILVLTFSLFLSS
                     PFSLILGLIAYLIAGHRVIRKAMKEIASRQIFTENFLMTLATAGALYLGELIEASAVM
                     ILYEIGLYLESGAIAKSRKNLQALLALKPEKAHLKTENGIEDRKLNTIQKNQIILVYA
                     GERIPLDGVVIKGESTVDTSSLTGEAIPVFVEPKSSVFAGFLNGSGILEIQVQNSEAE
                     SMVSRIIKLIENASAKKSDTEKFMTRFAKYYTPAVVLAAVLVFAIPSLLGYSYKIWLQ
                     RALIFLVVSCPCALVISIPLTYYIGIGKSAKSGIIFKGSIYLDLLSRVKTLIFDKTGT
                     LTTGELSIAEIKTVPDIDQEELKLSTWLCEWTSSHPFAKAVKKAFSYKFEPSKVDAFS
                     EFPGKGILLVYNQDRYLCGSEVFLESFGFLNLLDAGDNSVVHTVKNDIYLGCISFVDE
                     LKPGMKEVLTELKQKGVKHLSVLSGDRFPKVEKVAKELGLDSFYAALTPESKLTKLEE
                     IINSSQDIVAYCGDGLNDAPVLARADVGIAMGKIGAQASIETADVVLLNDKPEQLKSA
                     FGISKETNKMVWQNIFLALSIKMLVMILGIAGLAQLWEAIIADVGVTLLVIFNSLRMM
                     KHPDG"
     gene            complement(2370..2723)
                     /locus_tag="PLG69_05640"
     CDS             complement(2370..2723)
                     /locus_tag="PLG69_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPI43028.1"
                     /translation="MKRQNCLCKSISVEEMEDIKTHIPEDETIQKMADFFKVFGDGTR
                     LKILYYLSRHELCVADLALLVQMQQSAISHQLKLLRLDRLVKYRKEGTTTYYSLDDEH
                     INSIFKVALEHSQER"
     assembly_gap    3165..3264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3477..4025
                     /locus_tag="PLG69_05645"
     CDS             3477..4025
                     /locus_tag="PLG69_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43029.1"
                     /translation="MHLLNRMVICTLTLFSFACANIPKSRTEEEKRIILPEGETSANC
                     ITEEELESFSFNTLECVQALLTGVWFTRFEKSGRPLIFLCPPETEPALQEQGKNFIGL
                     FCADLLQTQAVNLVVCDSVFTLSENREKIMQLVKKSGADFFLQSIIAIEEGIPLLKLN
                     LYNVQNLAIVYSDTRSLLLIKI"
     gene            4038..4940
                     /locus_tag="PLG69_05650"
     CDS             4038..4940
                     /locus_tag="PLG69_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPI43030.1"
                     /translation="MQISKLDQMFDVLATRPKKRLVAAWGVDSHTILAAYMAVEMGIV
                     DATLVGDEKLILSVCREHNLDCSQFNIVHCPTDVKSAALAVETINKGEGDFLMKGLLS
                     TDRYMKAILNKECGLMDPGAILSHVTVAEPRNYHKLLIVGDVAIIPLPDLNQKIAITN
                     YLIKTAHFLGIENPKVGLLSASEQTLPKIPSSAEAAIIAKMAQRGQIKGAIVEGPLGF
                     DMIIDRESALIKGVDSPVCGDADCILFPNIEAGNTFYKTIIKLLKSELGAIVMGARVP
                     CVLTSRGDGERSKLYSIALAAMLA"
     gene            4943..5851
                     /locus_tag="PLG69_05655"
     CDS             4943..5851
                     /locus_tag="PLG69_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPI43031.1"
                     /translation="MITKLDELAEKAKNSGKKTRIAVAVAEDSNTINAILRATYEGFI
                     YPVLIGNEIKIKELLTSDIASNQFEIRNITEPNKAVKEAVGMVRNNEADVLMKGLIGT
                     DTFLKAVLDKEKGLLPPKAVMSYTCAIEIPKYHKLLFVSDTAVLIYPDLDQKIAMINY
                     SVAMARRFGIEKPKVALISATEKVSPSMQETLDYSLLCKMAERGQIKNCIVDGPLDIF
                     LACDPNSLAIKGIQSPLEGDADILIFPNLECANSFYKGLMLFGEGELAGLICGTTKPV
                     IVMSRSESENSKYYCLALSCLMAEEK"
     gene            5848..6279
                     /gene="rpiB"
                     /locus_tag="PLG69_05660"
     CDS             5848..6279
                     /gene="rpiB"
                     /locus_tag="PLG69_05660"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPI43032.1"
                     /translation="MKIAIASDHAGFEMKEAIKNAFPEIEFVDFGTHNTESMDYPDTG
                     FPAAKAVAEKQCEKGILICGSGIGMSITANKVKGIRAALCGNTDVARLSRLHNDANIL
                     VLAGRFTAIPYAKEITKVWLNTSFEGGRHLNRINKIQQGER"
     gene            6279..7571
                     /gene="glyA"
                     /locus_tag="PLG69_05665"
     CDS             6279..7571
                     /gene="glyA"
                     /locus_tag="PLG69_05665"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPI43033.1"
                     /translation="MNHIQKTDPEIYAAIMNELKRERENLELIASENFTSLAVLETQG
                     CVMTNKYAEGYPYRWSKKTGAINYNLYGRYYGGCEFINDAERLAIERAKQIFGAEHAN
                     VQPHSGSQANMAAYFTLVKPGDTVLALELSHGGHLTHGHPLSFSGQLYNIIPYTVNKE
                     TEQLDYDELEALAMEHKPQMILAGASAYPRKLDFARFREIADKVGAKLMVDMAHIAGL
                     VAVGLHQSPVPYADIVTSTTHKTLRGPRAGLILCKEEFAKEVDSKVFPGVQGGPLMHI
                     IAAKAVAFQEALQPEFKIYQQKVVENAETLANALIKKGFKLVSGGTDTHLMLIDLGPE
                     VNGSPSGKKMEEALDKAGITANKNTVPFDTRSPFVASGIRLGTPAVTTRGMGKAEMEQ
                     IADLIKRVYDHIDNEEYLQELKKEVHSLTDKFPLYPEL"
     gene            7930..>8618
                     /locus_tag="PLG69_05670"
     CDS             7930..>8618
                     /locus_tag="PLG69_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HPI43034.1"
                     /translation="MRIAAIDCGSNSLKCLIAEKRGEDFNCLKDLRFQTRLASALNTN
                     GFLSATAIEKTITILKHLQENEFPAYQIEEIRAVGTEAIRQAANKQEVVDRIFRETGI
                     QLSVINEAQETFLEWKGVLSGLKKPLQPITIFDSGGASTEIINGNKGLIHKATCFPVG
                     AVNFTQRYVHSDPISPTDFEELVRKIFQSLPQSFMIQDTFIGAGGGVYVCACVASGGK
                     INSPEEAEGYE"
BASE COUNT         2703 a         1609 c         1663 g         2543 t
ORIGIN      
        1 atcgctttgc gtgataactg ccagcgtagt tgttaatcag ctaaaaatta tcaggtgtaa
       61 gttatttccg attttgttgt tctcttgaag cagtaatgat caagtaacaa ccaagtaaca
      121 attccctaac gagataacgg aagtgttacg gaagtgttac ggagtcgtta ctgaatgttc
      181 taaatatcaa taagttagct atcaacattt aggagaggaa gaaaaaggac atattataga
      241 ttttatctct tgtatatcaa tggattaggg aatattaaat caaataagaa tggttgataa
      301 agatgtcaac catccgggtg tttcatcatt cttaaggaat tgaaaataac cagtaaagtg
      361 acacctacat cggcaattat tgcttcccag agctgagcca aaccggcaat tcccaaaatc
      421 atcaccagca ttttaatgct taaagccagg aaaatattct gccagaccat tttattagtt
      481 tctttagaaa tgccaaaagc gctttttaat tgttcaggtt tatcgttcag taaaaccaca
      541 tcggctgttt caatagatgc ctgagcgcca atttttccca ttgcgattcc cacatctgct
      601 ctggctaaaa caggtgcatc attaagacca tcaccgcaat aggcaactat atcctggctg
      661 gaatttataa tctcttccag tttggttaat ttactttccg gggttaaagc agcataaaag
      721 ctatccaaac ccaattcttt ggcaactttt tcaactttgg gaaagcggtc tccggataga
      781 acgctcaagt gttttacgcc cttttgttta agctctgtta gcacttcttt cattcctggt
      841 ttcaattcat ctacaaaact gatacagccc aaataaatat catttttaac ggtgtgaact
      901 acgctgttat cgcctgcatc aagcaaattt agaaaaccga aggattctaa aaagacctca
      961 cttccgcaaa gataacggtc ttgattgtaa accagcaaaa ttccctttcc gggaaattct
     1021 gaaaaggcat ctaccttgga gggctcaaat ttgtaagaaa atgccttttt aaccgccttc
     1081 gcaaaaggat gtgaggaagt ccattcgcaa agccaggtac ttagtttcag ttcttcctgg
     1141 tcaatatccg gaactgtttt tatttcggca atactcaatt caccggtggt taaagttccg
     1201 gttttatcaa aaattagggt tttaaccctg ctaagtaaat ccaaataaat gctccccttg
     1261 aaaatgatac ccgattttgc ggacttacct attccgatat aataagttaa aggtatggaa
     1321 atgactaagg cacaaggaca ggaaacaacc agaaaaatta gggcacgctg caaccaaatt
     1381 ttgtatgagt aacccaaaag tgatggaata gcaaaaacca gaactgcagc taaaaccaca
     1441 gcaggtgtat aatatttagc aaaacgggtc ataaactttt cggtatctga ctttttggca
     1501 ctggcatttt caattagttt tataatgcgg gaaaccatac tttccgcttc actattttgt
     1561 acttgaattt ccagaattcc gctgccattc aaaaatccgg caaaaacaga ggactttggt
     1621 tctacaaaaa caggaattgc ttctcccgtt aaagatgatg tatcaaccgt tgattctcct
     1681 tttataacaa ccccgtccaa aggaattcgt tcacctgcat aaaccagaat aatctggttt
     1741 ttttgaatgg tattcagctt gcggtcttca attccatttt ccgttttcag atgtgctttt
     1801 tcgggtttta atgccagaag cgcttgcaaa tttttccggc ttttggcaat agctccgctt
     1861 tccaaatata atcctatttc atataaaatc ataactgctg atgcctcaat cagttcacct
     1921 aaatataacg ctcctgctgt agctaaagtc atcaaaaaat tctcggtaaa tatttggcgg
     1981 gatgctattt ccttcattgc ttttctaata accctgtgac cggcaataag ataggcaatt
     2041 aatcctaaaa taagagagaa aggactgctt aaaaacaaac tgaaagtcag caccaaaata
     2101 gctacaataa taaaccacgg ctttttttcc gtcctttcct gttccaaacc tgccagagta
     2161 aattggacat taggatcaat cttgctcgct atggcattta agcgttttag aggattatct
     2221 accttctcaa tgtattcaat ggtgagatgt ttattaataa aatccaaatt ggcactttcc
     2281 acttcctcca aattgttgat ttcagcttct attttagcac cgcaatcggg acaatcaaga
     2341 ttgtgaacat catactcttc aataaccatt tatcgctcct gtgaatgctc cagcgctact
     2401 ttaaaaatgc tgtttatatg ttcatcatcc agagaataat aggttgtagt accttctttg
     2461 cgatatttca ccagacgatc caaacgcaat aatttaagct gatgcgaaat tgccgattgc
     2521 tgcatttgaa ccaacagggc taaatccgct acacacaatt cgtgtctgga aagataatac
     2581 aatattttta aacgggttcc atccccgaaa accttgaaga aatcagccat tttttggata
     2641 gtttcatctt ctggaatatg tgtttttatg tcttccattt cttctacgga aatgctttta
     2701 cataaacagt tttgccgttt catttcagct cctttatgaa tatatgttca tatattgata
     2761 aacccgcttc cctgtcaaga aaaatcttcg caggaaaaat gggttggcaa cttcctattt
     2821 gccaaggagt tcgtagcact cagaaaaata aatgccttct atttatggag aatcataatt
     2881 ctgcagccaa aatgctaaat ttgattctct tttctccaag gttaaaacct atcgttaatt
     2941 tctgccgttc ctttgtccta tctgctcagg tagaaatcca gaaaaaaaaa acaatgtagc
     3001 catatttttt cgctgggtta aaacccgtcg ttaaaacctg tctttcctac ggaactcgtt
     3061 ctcaaaaaaa gagataagct gtatcctatt atccttccgc cgggttaaaa cccgtcgtta
     3121 atatctgtcg ttcctacgga actcgttctc agaaaaagag ataannnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnattcac cttcgcggaa tatttttcct taaaatccta
     3301 cccataatgt ttcttatgat taagctaact taacgccagt tagataatta ggaaaactcg
     3361 gaaaacaaat cttgacagaa ttaggaatta ataacataaa gaaccaaatt attttgagac
     3421 gctatgaaat gaagttaatt tgccttactg catacagttg cgctaaaagg aagaaaatgc
     3481 atttattaaa ccgaatggtt atctgcactt taacactttt tagttttgct tgcgctaata
     3541 ttccgaaaag cagaacggaa gaggaaaaaa gaattatcct tccagaaggg gaaacttctg
     3601 caaattgtat taccgaggaa gaattagaat ccttttcttt taatacgctg gagtgtgttc
     3661 aagcattgtt aacaggggtt tggtttaccc gttttgaaaa aagtggcaga ccgcttattt
     3721 ttctatgtcc tcctgaaaca gaacctgctt tacaggaaca aggcaaaaat tttatagggt
     3781 tattttgtgc cgacttactg caaacacaag ctgttaatct cgttgtttgt gattcagttt
     3841 tcactttatc ggaaaatcgg gaaaagataa tgcaattggt aaaaaaaagt ggtgcagatt
     3901 tctttttaca aagtataatt gcaatagaag aaggaattcc cctactgaaa ttgaatctct
     3961 ataatgttca gaacttggca attgtttata gtgatacccg cagtttatta ttaataaaga
     4021 tataaaggat gaaaactatg caaattagca aacttgacca aatgttcgat gttttggcta
     4081 ccaggcctaa aaagcgtctg gtagctgcct ggggtgttga ttcccatact attttggctg
     4141 cctatatggc tgtagaaatg gggatagttg atgctacctt ggttggcgat gaaaaactga
     4201 tactttccgt ttgccgggaa cataatcttg attgttctca atttaacatt gtccattgcc
     4261 ctactgatgt taagtccgct gctttggctg tagaaacgat taacaagggt gaaggcgatt
     4321 ttttaatgaa aggtcttctt tccacagacc gctatatgaa agcaatattg aataaggaat
     4381 gcggattgat ggatcctggt gctattttat cccatgtaac agttgccgaa ccgagaaatt
     4441 atcataaatt actaattgtt ggtgatgtag ctattatacc tttaccggat ttgaatcaga
     4501 aaattgccat taccaattat ttaattaaaa cggctcattt tttagggata gaaaatccga
     4561 aagtaggact tctttccgcc agcgaacaaa cccttcccaa aattccttct tccgctgaag
     4621 cagctataat tgccaaaatg gctcagcgcg gtcaaatcaa aggtgcaatt gtagaaggtc
     4681 ctttgggatt tgatatgatt attgatagag aaagtgctct tattaaaggt gtagatagtc
     4741 ctgtttgcgg agatgcggat tgcattcttt ttcctaatat tgaagcaggc aatacttttt
     4801 ataagaccat cattaaactg ctgaaaagtg aactgggagc aatagttatg ggtgcgcggg
     4861 ttccctgtgt tttaacttcc cggggtgatg gagaacgcag taaattgtat tctatagctt
     4921 tagcggctat gttggcgtaa aaatgattac caaacttgat gaactggcag aaaaagctaa
     4981 aaacagcggc aaaaaaaccc gcattgcagt tgccgttgca gaagatagca ataccattaa
     5041 tgctatttta cgagctactt acgagggctt tatttatcct gttctgattg gcaatgagat
     5101 aaaaatcaaa gaactactga cctctgatat tgcctctaac caatttgaaa taaggaatat
     5161 aacagaacca aacaaagctg taaaagaagc tgttggaatg gttcgcaata atgaagccga
     5221 tgttttaatg aaaggtctga taggaaccga tactttcctg aaagctgttt tggataaaga
     5281 aaaaggactt ttacctccga aagcggttat gagctatacc tgtgctatag aaattcccaa
     5341 ataccataaa ctgctttttg ttagcgatac agctgtgtta atttacccag atttagacca
     5401 gaaaatagca atgattaatt atagtgtagc tatggcaagg cgttttggta tagaaaaacc
     5461 gaaagtggca ttaatatctg ccaccgaaaa agttagccct tcaatgcaag agaccttgga
     5521 ctattctctt ttatgtaaaa tggcagaaag aggtcaaata aaaaactgca ttgtagatgg
     5581 tcctttagat atttttcttg cttgcgatcc taattccctt gctattaaag gaattcaaag
     5641 tcccttggaa ggggatgccg atattcttat tttccctaat ctggaatgtg ccaatagttt
     5701 ttataaaggt ctgatgcttt ttggagaagg ggaactggca ggtttaattt gtggaacaac
     5761 aaaacccgtt attgtaatga gtagaagcga aagtgaaaac tctaagtatt actgtttagc
     5821 gttatcttgt ttgatggcgg aggaaaaatg aaaatagcca ttgcttccga tcatgccggt
     5881 tttgaaatga aagaagcaat caaaaatgct tttccggaaa ttgaatttgt ggattttggc
     5941 actcataata cagaaagtat ggattatcct gataccggtt ttcctgctgc taaagcggtt
     6001 gcggaaaaac aatgtgaaaa aggtatctta atttgtggca gtggaatagg aatgagcatt
     6061 actgccaaca aagtgaaagg cataagagct gctttatgtg gaaatacaga tgtagcacgc
     6121 ctttcacggc tgcataatga tgccaatatt ttggtcttag caggaagatt tactgccatt
     6181 ccttatgcca aagaaattac taaggtctgg ctaaacacct cttttgaggg aggaagacat
     6241 ttaaatagaa ttaataaaat acaacaagga gaaaggtaat gaaccatatt caaaaaaccg
     6301 atcccgaaat ttatgccgct attatgaacg aactaaaaag agaacgggaa aatctggaac
     6361 ttattgccag cgagaatttt acttccctgg ctgttttgga aactcaggga tgtgtgatga
     6421 ccaataaata tgctgagggc tatccctatc gctggagcaa aaaaaccgga gctattaatt
     6481 acaaccttta cggaagatat tatggcggtt gtgaatttat caacgatgct gaacgcttgg
     6541 caattgaacg tgcaaaacaa atttttggag cagaacatgc caatgttcaa ccccatagcg
     6601 gttcccaggc aaatatggct gcctatttta cacttgtaaa accaggtgac acggttttgg
     6661 ctttggaact atcccatgga ggtcatttaa cccatggcca tccactttcc ttcagtggac
     6721 aactttataa cattattccc tacactgtaa acaaagaaac ggaacaattg gactatgatg
     6781 aactggaagc tttagcaatg gaacataaac cccaaatgat tttagccggc gcttctgcct
     6841 atccccggaa attggatttt gccagatttc gtgaaattgc cgataaagtt ggagctaaac
     6901 ttatggttga tatggctcat attgcaggac ttgttgctgt aggtttacat caaagccctg
     6961 ttccttatgc ggatattgtt acttccacaa ctcacaaaac cttacgcgga cctcgtgctg
     7021 gacttatttt atgcaaagaa gaatttgcca aagaagtgga tagtaaggtt tttcccggtg
     7081 tgcaaggcgg tcctttaatg catattattg ctgctaaagc agttgctttt caagaagccc
     7141 tgcaaccgga atttaaaatc taccagcaaa aagtggtgga aaacgctgag accttagcca
     7201 atgccttaat taaaaaagga tttaaactgg tttcaggcgg aactgatact cacctgatgc
     7261 tgattgattt aggtcctgaa gttaatggca gccccagtgg taaaaaaatg gaagaagcac
     7321 ttgataaggc aggaattacg gctaataaaa atactgttcc ctttgatacc agaagtcctt
     7381 ttgttgcctc cggaattcgt ttgggaactc ctgctgtaac cactcgcgga atgggaaaag
     7441 cggaaatgga acagattgcc gatttaatta aaagggtcta tgatcatatt gataacgaag
     7501 aatacctgca agaattgaaa aaagaagtgc attccctgac agataaattt cccctttacc
     7561 ctgaactgta gacggaggac actgttactc cgcaaaaaaa tgaaaaagta aaaaggttaa
     7621 aaagtgaaag ggttgaaagg tgaaaaagtg aaactcggaa tgaacattat ggtaaaaagt
     7681 gcggagcttc agcgagctcc gattacacta caattataga gtcgccactg ctttaagcca
     7741 ggagtcgtca ccgccgagat aactttgacc ttacttgcag acaattttgg tggtgaggac
     7801 tcctgacaat ttttagcacg cagattcttt attgattata agataggaga agaaaatgac
     7861 tatagctaaa atttatttaa ggataaacaa atcctgttca acctggttac attttcacct
     7921 gttaatccta tgcgcattgc agcaatagat tgcggttcca attctcttaa atgccttatt
     7981 gcagaaaaga ggggagagga ctttaattgt ttaaaagacc taagatttca aacccgtctt
     8041 gcttctgctc taaatacgaa tggtttcctc tctgcaacag ccatagaaaa gacaattact
     8101 attcttaaac atttgcagga aaatgaattc cctgcttacc aaattgaaga aattcgcgct
     8161 gttggcacgg aggctattcg ccaagccgct aataaacagg aagttgtaga ccgcattttt
     8221 agggaaacag gtatccaatt atccgttatc aatgaagccc aggaaacatt tttggaatgg
     8281 aaaggagtgc ttagcggttt gaaaaaaccc ttacagccaa taactatttt tgacagcgga
     8341 ggtgccagca ctgaaattat taacgggaac aaaggactta tccataaagc gacttgtttt
     8401 cctgtaggag ctgtgaattt tactcaaaga tatgttcaca gcgatccgat ttccccaact
     8461 gactttgagg aattggttag aaagattttc caaagtttac cccaatcctt tatgatacaa
     8521 gatactttta tcggtgccgg aggaggggta tatgtatgtg cctgtgtggc ttccggaggg
     8581 aaaattaatt cacccgaaga agccgagggc tatgaaat
//
LOCUS       DAOPFL010000071         8574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_10379_length_8574_cov_108.013382, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000071 DAOPFL010000000
VERSION     DAOPFL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10379_length_8574_cov_108.013382"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="rsxC"
                     /locus_tag="PLG69_05675"
     CDS             complement(<1..1165)
                     /gene="rsxC"
                     /locus_tag="PLG69_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPI43035.1"
                     /translation="MRLKTFPGGVHPPDEKRYSASVPITEAPIPQKVIIHLSQHIGSP
                     SKPIVAVGDEVLTGQKIAEATGYVSLVQHSSISGKVTAINRFPNATGSSSMAIQITGD
                     GSDKWIELVDEPNVMDLPLEEMKKRIGEAGICGMGGAGFPTLVKLSPPADKPIDTVIL
                     NGVECEPYLTSDHRLMLEKGEEILIGLKLIMKILSAKKGMIGIEANKPDAIAKMQELT
                     KNEPAMEVVPLKLRYPQGAEKQLIYAATKRKVPAGGLPLEVGIVVQNVGTAFAIYEAI
                     RYQKPLIQRVITVTGSPVTKPQNLLARIGTPFSELVDFCGGTKEEVGKVICGGPMMGF
                     ALPTLEATVGKGSGGLVLMNTEDARLNEEGNCLRCARCVDVCPMNLVPCMIVSA"
     gene            1288..1494
                     /locus_tag="PLG69_05680"
     CDS             1288..1494
                     /locus_tag="PLG69_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43036.1"
                     /translation="MKNEEEKSGGEAVRIGQRYFQRKGKLDFCLFLNQFPTSTQPLGW
                     VDVGWRIGCWQGTSKNIFRKEQML"
     gene            complement(1764..3014)
                     /locus_tag="PLG69_05685"
     CDS             complement(1764..3014)
                     /locus_tag="PLG69_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPI43037.1"
                     /translation="MKVNLGNKKLLGINGLGRIGKLLLWNQLHLKYFDGIVVNCGRIV
                     GKTLDDLIQVIEMDSTYGSIHKFLYGVVGKKAEIKILDAEEPLLEIEGMLVKILRTAR
                     DPKDINWLNEGVRIVVDCTGNFLDPTMHTDSGKPCLRGHLDAGAIKVICSAPFKIKSG
                     NTFPDDAKTMIYGINHLEYDPLKHNIISAASCTTTGLAHMLKPLLENQKTSRILTASM
                     STIHSATNTQSILDSVPKTDASDLRKSRSVLNNIILSTTGVTKALEQVMPNIKHIGFM
                     ADSVRIPTTTVSLINLNVTFHTELDELGEPLVNRKLINEVYQKAAENSQKDLLLISHR
                     QNVSADMIGTMAAVVIEAHETHTRTGFITIPSSTLAEQGILTDKVLEMPVTHAKIFGW
                     YDNELGSYVNCLSRLTNYIEEKTL"
     gene            complement(3044..7123)
                     /locus_tag="PLG69_05690"
     CDS             complement(3044..7123)
                     /locus_tag="PLG69_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPI43038.1"
                     /translation="MEPLKSKALAENLAITKVAEIVLDDEAKWLLSLTEGNFGINQRC
                     RNFLEELYHPYANPEAVASLIRQSILGDLWFYVQLPDKEKTLTVIGDLYLLAAKKCTK
                     PMQEKRIIIEYLDFISALCDKEGIPEGVYAKLLNELDNWQKNSRELFIPLNGACRKCL
                     SNLVQVYKDNNICFRMLKDLLKEGLEFWQKTTDIEGWCAKMGAESNLDVKQLSSTLGK
                     KYYQKWLRQLEEIQDFASLKDIPSFSDIAVLQREAICKFSTLTLRIQYIFYLLGLPGM
                     EDLRNHLLWDLNRQLADLEKDLKEDEVPLMLDSIFETLYEFKETHLSIVLDCILTIGK
                     TVLSHKNKKHCQHLLPKIMELEFTPPGKIKIDKDWQMEVDKNHIKHLRMLLELIAIDP
                     FMCKDLLAYLIISLSQKGVFISDTDLFQKDVSAFLNSNFKPLVVQTKHLLKMFPVFFN
                     EIGAEGEIRDISTSLDELGHRKDLLMHFLRKQLHTESNNTHLLLIEKILQYWTNLDYR
                     ELQDMIPEDVLIYVMHPDEMTKKQSAVVNKFLQKHNLTPEELLSLSWQRVSKLFENEP
                     EDFSLKRLKLLCYSHFLLKDKYNLDPYDIVKFLTRYSWFDAHEHNRLANSFMRKDYDS
                     SIRQLLSYIGKMNSVILDPQPTIAWENIYYKRHIAAGIPSMYGMYREPKLEAMGMIFR
                     IENVVKRLFERNVQQLNLSYITGKTLKRIIRILELYDYAMKQILVTSDAYGSALNMLR
                     SAQYLSNLSIDQYLDIFKLLKDSVVEIINEYYYRFYEGQLRQIGMRIAEKEKGKKGRG
                     TKEIEIFAEEFYRNLLSKSFLVQGLDNFITRILESLSAMKNLFKPEDILKVMSYDPDK
                     LFFHLNKKNSRIENQVLLGSKAYFLKRMYQYEFPVPPGFVITTDLFRHRNIINTHPDI
                     SNELDNLILQNLNILEKNTGLVLGDPERPLLLSVRSGAPMSLPGAMNTFLNIGLTDEV
                     TYKLSRRPNYGWTAWDCYRRLIQSWGMAFGIDRDEFDAVMLNYKKKYNVELKTQFSDR
                     QMQDMVQDYQKVLAKHNIELEQQPFMQLYKAISNVLDSWNTDRARLYRQKLHIADEWG
                     TAVIIQKMVLGNISLESGTGVLFTYADFNEEGGICLNGDFTLCSQGEDVVAGLVHTLP
                     ISEMQRLHSKTAVSSSLENTFPAIYNALMRYVKQLIEDKNYPHQEMEFTFEGPSPDQL
                     YILQTRDQVIQKAPDYKVFGTTKGEQKKLAYGIGIGKGAVNGIIVFGKEDIEKFAGKG
                     EALILVRPDTVPDDMELLFACQGLLTSRGGVTSHAAVTATRLGLIGVVNCRDLVVSES
                     DSNCRIGSTYLKAGDKIALDATSGAIYLGHYPLVSVHCL"
     gene            7370..8539
                     /gene="dnaN"
                     /locus_tag="PLG69_05695"
     CDS             7370..8539
                     /gene="dnaN"
                     /locus_tag="PLG69_05695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPI43039.1"
                     /translation="MRLAIEKKDLLARIQHLVSIVPTKNTSPILNNYLIEVSEENNKV
                     RISTTDLELMVVVEFPAAVSEGGTVAVSAHHFNEIINYMPDGVINLWRNDDLLMIQCG
                     KVDFNLLIADHTLFPVIPEPKLNNPITIDAELFSRMISKTYFAVSTDVNRPVLNGVCW
                     KIYPDHHIMAATDGRKVAEIKILNSSLLSSLEPNDNPEMNIFTEQNQYYVEKVIPVKT
                     LLFLQKIFNDEVKELKVGLEHNRILFLYGEYFISSQVLEHKFPDYQKAFPAELPNKFV
                     IDRQALITAIKRVALVAPDDNLRIHFDLDSDRFEVNTSNRDTGEAKENMDDFTFAGSS
                     TGISFNYKYMLSILEAIDTEKVVIKLGTSKDAMMIYNETPLPDQEITFLLMPLRS"
BASE COUNT         2561 a         1742 c         1534 g         2737 t
ORIGIN      
        1 cggcagaaac aatcatacag ggaaccaaat tcatcgggca aacatctaca cagcgggcac
       61 aacgcaaaca atttccttct tcatttaaac gggcatcttc agtattcatt aaaaccaatc
      121 cgccgcttcc ttttcccacc gttgcctcta aagtggggag ggcaaaaccc atcattggtc
      181 cgccacaaat aacttttcct acttcttctt ttgttcctcc gcaaaaatca acgagttcgg
      241 aaaaaggagt tccaatcctg gctaaaagat tttggggctt tgtaacggga gaaccggtta
      301 ccgttattac tctttggatt aacggctttt gataacgaat cgcttcataa atggcaaagg
      361 cagttcctac attttgcact acaattccaa cttccaaagg aagaccacca gcaggaactt
      421 ttcttttggt agcagcatag ataagctgtt tttcggcacc ttgagggtaa cgcaatttta
      481 agggaactac ttccatagca ggctcatttt tagtaagttc ttgcatttta gcaatggcat
      541 cgggtttatt ggcttcaatt ccgatcattc cttttttagc acttaaaatt ttcattatca
      601 gttttaagcc gattaagatt tcttcacctt tttccagcat caaacgatgg tcgctggtta
      661 aatatggttc gcattctact ccgttcaaaa taaccgtatc tatcggttta tctgcaggag
      721 gagaaagctt taccaaagtt ggaaaacctg caccacccat tccgcaaata ccggcttcac
      781 caattctttt tttcatttct tccaggggta aatccataac attcggttca tcaacaagtt
      841 ctatccattt gtcggaacca tctccggtaa tttgaattgc catagaactg gaacctgtag
      901 cattaggaaa acggtttatg gctgtaactt ttccggaaat tgaagaatgt tgaacaagcg
      961 acacataacc tgttgcttcc gcaatttttt gacctgttaa aacctcatca cccacagcta
     1021 caattggctt tgaaggtgag ccaatatgct gggaaagatg aataattact ttttggggta
     1081 ttggagcttc agttataggc acagaggcac tatacctttt ttcatccgga ggatgaacac
     1141 ctccgggaaa tgttttcaag cgcattaaca ctccctttgg cttgaatttt tcaagtcctg
     1201 ataatcaatt tttggtcgca tttttataga tacgcattta taggcaagta aattttttct
     1261 caagtgggaa taaatgacga aagcaaaatg aagaatgaag aggaaaaaag cggaggcgaa
     1321 gcagtgagaa tcggacagag atattttcaa aggaaaggaa aattagattt ttgtttgttc
     1381 cttaatcaat tcccaacctc aacccaacct ttaggatggg ttgatgttgg gtggaggata
     1441 ggttgttggc agggaacaag taaaaatatt ttcagaaagg aacaaatgct ctgataaaac
     1501 caaacaacaa aattgaaggt attgggagag ggaaaattcc gcttttagtt agtataaaaa
     1561 aggcaaataa aaaatttatc aaaaagaagt tgccgaaccg gaattcggca acctcaggta
     1621 ataatatacc ttctaagtgg gtaaattaca ttcttcaggt ttagcgcggg tttcctcttt
     1681 tgtctttttc acttcactca atcacccgtt aacctgttcc tttgctaact tgcttacctc
     1741 tccatccgtt cactcgttag cttttaaagc gttttttcct ctatgtaatt ggttaatctg
     1801 gaaaggcaat tgacataaga gccaagttca ttatcatacc agccaaagat tttagcatga
     1861 gttacgggca tttccagaac tttatccgta agaatacctt gttcagccag ggttgatgaa
     1921 ggaatagtaa taaaaccggt gcgggtatgt gtttcatgtg cttcaataac aactgcagcc
     1981 atagttccaa tcatatctgc ggaaacattt tgtcggtggc tgattaacag caggtctttt
     2041 tgggagttct cagcagcttt ttgatacact tcattgatca gtttacggtt tactaacggc
     2101 tctcccagct cgtccagttc cgtatgaaaa gtaacattta agtttataag agaaacagtc
     2161 gtggttggaa ttcggacgct atcagccata aaaccgatat gtttaatatt gggcataact
     2221 tgttccagtg ctttagtaac gccggttgtg gaaagaatta tattatttaa aacgcttctt
     2281 gattttctta agtcggaagc atcggttttc ggaacactat ccaaaatgga ttgagtgttt
     2341 gtagcagaat gaatagtact catagaagcg gttaatattc gggaagtttt ttgattttcc
     2401 aaaagcggtt tcaacatatg tgccaagccg gttgtagtgc aggaagccgc tgaaattata
     2461 ttatgtttta ggggatcata ttccagatgg ttaatgccgt aaatcattgt tttggcatca
     2521 tctggaaacg tatttccgga ttttatttta aaaggtgcgc tgcagataac ttttatggct
     2581 cctgcatcta aatgtcctct taaacagggt tttccagaat cagtgtgcat tgtagggtcc
     2641 agaaaatttc ctgtgcaatc aaccacaatt cttacccctt cattaagcca gttaatatct
     2701 ttagggtcac gcgcggttct taaaattttt accagcattc cttcaatttc caaaaggggt
     2761 tcttcagcat ccaggatttt aatttccgct ttttttccta caactccata gagaaactta
     2821 tggatagaac cgtaagtgct atccatttct attacttgaa taaggtcatc cagggttttg
     2881 cctacaattc ttccacagtt taccacaatt ccgtcaaagt acttaaggtg taactggttc
     2941 cataacagca gttttccaat tctacccaga ccgttaattc cgagtagttt tttgttccct
     3001 aagttcactt tcattttgct ttctccttct atcttttatt gtattataaa caatgcacac
     3061 taacgagagg ataatgcccc agataaatag ctccactggt agcatccaga gctattttat
     3121 cacccgcttt taaataggta ctgcctattc tgcaattgga atccgattcc gaaactacca
     3181 aatcccgaca atttacaact ccgattaagc ccaaacgagt agctgtaacc gctgcatgag
     3241 aagttactcc accccttgaa gttaacaatc cctggcatgc aaaaagaagt tccatatcgt
     3301 caggaaccgt atcaggacgc actaaaataa gggcttcacc cttaccggca aatttttcaa
     3361 tatcctcttt gccgaacaca ataattccgt tcacagcgcc ttttccgatg ccaatgccgt
     3421 aagcaagttt tttttgctct ccttttgttg ttccgaagac cttatagtcc ggagcttttt
     3481 gaataacctg atcgcgagtt tgtaaaatat ataactgatc gggagaagga ccttcaaaag
     3541 taaattccat ttcctgatga ggataatttt tatcctcaat aagctgtttc acatagcgca
     3601 tcaaagcatt gtagattgcg ggaaaggtat tttccaggga actggaaacc gctgttttgc
     3661 tatgtaaacg ctgcatttcg gaaataggta aagtatgcac taaaccggct acaacatcct
     3721 ctccctgact gcacagcgta aaatcgccat ttaaacaaat tccgccttcc tcattaaaat
     3781 ccgcataagt aaaaagaact ccggttccgg attccagaga tatatttcct aaaaccattt
     3841 tctgaataat aactgcagtt ccccattcat cggcaatatg caatttttga cgatataatc
     3901 gggctctatc cgtattccag ctatccagaa cattggaaat tgctttataa agttgcataa
     3961 agggttgctg ttccagttca atattatgtt tagccagcac tttttgataa tcctgcacca
     4021 tatcctgcat ctgcctatct gaaaattggg tttttagctc aacattatac tttttcttat
     4081 agtttagcat aacggcatca aattcatcgc ggtcaattcc aaaagccata ccccagcttt
     4141 gaattaaccg tctgtaacaa tcccaggcag tccacccata attaggacga cgggaaagtt
     4201 tatatgtaac ttcatcagtt aaaccaatat tcaaaaaagt gttcattgct ccgggcaaac
     4261 tcattggagc tcctgaacgg acagaaagca ataacggtct ttccggatcg cctaaaacaa
     4321 gccctgtatt cttttccaaa atgtttaaat tctgcaaaat taagttatcc aattcgttag
     4381 atatatcggg atgtgtatta ataatattcc gatgacggaa aaggtctgta gtaatcacaa
     4441 aacctggagg aaccggaaat tcatattgat acatccgttt cagaaaatat gctttagaac
     4501 ccaaaagcac ctgattttct atgcgggaat tctttttatt taagtggaaa aagagtttat
     4561 cgggatcata gctcattact ttgagaatat cttccggctt aaaaagattt ttcatcgcag
     4621 ataaggattc caagattcgg gtgataaaat tatccaagcc ctgcaccaga aaggatttgg
     4681 aaagcagatt acgataaaat tcttcggcaa aaatttctat ttccttagtt cccctacctt
     4741 ttttcccttt ctccttttct gctattcgca ttccaatttg cctcaattgc ccttcgtaaa
     4801 atctataata gtattcgttt ataatttcca ccacactatc tttcagcaac ttgaaaatat
     4861 ccagatactg gtctatggaa agattgctga gatattgagc tgatcttaac atattcaaag
     4921 cagaaccgta agcatcactt gtaaccaaaa tctgtttcat tgcataatcg tataattcca
     4981 atatccggat aattcttttc agggtcttgc cggtaatata gctcaggttc aattgttgca
     5041 cattgcgttc aaaaagccgt ttaaccacat tttctattct aaaaatcatt cccattgctt
     5101 ccagtttcgg ttcacgatac attccataca tagaaggaat gccggcagca atgtgtcttt
     5161 tgtaatatat gttttcccag gcaatagtag gttgtgggtc taaaataacg ctgttcattt
     5221 tgcctatata ggaaagcaat tgcctaattg aactgtcata atcctttctc ataaagctat
     5281 tggcaaggcg attatgttcg tgagcatcaa accagctgta acgggttaga aattttacaa
     5341 tatcataagg atccagatta tacttgtcct tcagcagaaa atgcgagtaa catagaagtt
     5401 tcagtcgttt taaagaaaag tcctccggct cgttttcaaa taatttacta accctttgcc
     5461 aggaaagaga aagcagctct tcaggagtta agttatgctt ttgtaaaaac ttattaacca
     5521 ctgcactttg cttttttgtc atttcatccg gatgcatcac ataaatgaga acatcttccg
     5581 gaatcatatc ctgcagttct ctataatcta aattagtcca atactgcaaa attttctcaa
     5641 ttaaaaggag gtgagtgtta ttgctttcag tatgtaactg tttgcgtaaa aagtgcatta
     5701 agaggtcttt tctgtgtccc aattcgtcca gagaggtact tatgtccctt atttcacctt
     5761 ctgctccaat ttcattgaaa aagacgggaa acattttcag caggtgtttg gtttgcacta
     5821 ccaggggctt aaaattggaa ttcaaaaagg cggaaacatc tttctgaaag agatcggtat
     5881 cggaaataaa aacccctttt tggcttaaac tgataattaa ataggcaagc aggtctttac
     5941 acataaaggg atcaatagct ataagttcca gcagcattct aagatgtttg atatgatttt
     6001 tatccacttc catctgccag tccttatcta ttttaatctt tccgggaggg gtaaattcca
     6061 gttccattat tttagggagc agatgttggc agtgtttctt gtttttatga gaaagcactg
     6121 ttttgccaat agttaaaata caatccaaaa caatggaaag atgtgtttcc ttaaattcgt
     6181 ataaggtctc aaaaatggaa tccaacatca aagggacttc atcttccttc aagtcctttt
     6241 ccaaatctgc taattgacga ttcaaatccc aaagcagatg attacgcaaa tcctccattc
     6301 cgggtaaacc caaaagataa aatatatatt gtattctcaa ggttaaggtg ctaaatttac
     6361 aaatcgcttc tcgttggaga acagctatat cggaaaaaga gggaatatct ttcagggatg
     6421 caaaatcctg aatttcttca agttgcctaa gccatttttg ataatatttt ttgcccaagg
     6481 ttgaggaaag ctgtttcaca tccaagttgc tttccgctcc catttttgca caccagccct
     6541 ctatatctgt tgttttctgc caaaattcca agccctcttt taacaggtct ttcagcatcc
     6601 tgaaacaaat attgttgtct ttatatacct gcaccagatt gcttaaacat tttctgcaag
     6661 caccatttaa aggaataaat aactccctgc tgtttttttg ccagttatcc agttcgttta
     6721 gtaacttggc ataaacgcct tccggaatgc cttccttgtc gcataaagcc gaaatgaaat
     6781 ccagatattc aataataatt ctcttttcct gcattggctt agtgcatttt tttgccgcca
     6841 gtaaatacaa atcccctata acggttaagg ttttttcctt atccggcaat tgaacataga
     6901 accataaatc acctaaaata ctttgccgaa ttagggatgc tactgcttcc ggattagcat
     6961 aaggatgata cagttcttcc aaaaaattcc ggcagcgttg attgatgcca aaattacctt
     7021 ccgttaagga aagtaaccat tttgcctcat catcaagcac aatttcggct actttagtaa
     7081 ttgccagatt ttctgctaat gccttagatt tcaggggttc caaaatccaa tcctcatttt
     7141 ttgttatttt ctaaaataca ctctgcttct tgataaattt cgcttgcttt ctgtcaagga
     7201 gtttgtagct catattactt aaaacaagaa tttccgcatt aaaattccct tctgtgcctt
     7261 cggtgttttc gtgttctcgg tgtaaaatct cctttctcca aaaaagtgat tgactaaata
     7321 acacagtaaa tttttcttga aaaaagtaaa ttattccagt gaggttatta tgagattagc
     7381 gattgaaaag aaagaccttc ttgcccgcat tcaacatctg gtatccattg tgcctacaaa
     7441 aaacacatct ccgatattga acaactacct tattgaggtc tcggaagaaa acaataaagt
     7501 ccgaatcagc actaccgatt tggaacttat ggttgttgta gagtttcctg cagctgtttc
     7561 tgagggtgga acagttgctg tttctgctca tcattttaat gaaattatta attatatgcc
     7621 cgatggggtt atcaatcttt ggcgtaatga cgatctgctg atgattcaat gtggcaaagt
     7681 tgattttaat ctgttaattg ctgatcacac tctttttcct gttattcccg agccgaaact
     7741 gaataatccc ataactattg atgcagagtt atttagcagg atgatttcca aaacctattt
     7801 tgccgtttcc acggatgtaa accgtcctgt tctcaatggt gtttgctgga agatttatcc
     7861 tgatcatcat ataatggcag caactgatgg acgcaaagta gcagaaataa aaattctgaa
     7921 ttcgtccctt ttatcttctt tggaaccgaa tgataacccg gaaatgaata tttttacaga
     7981 acagaatcaa tattatgtgg aaaaagtgat tccggtaaag accctcctgt ttctgcaaaa
     8041 aatattcaat gatgaagtga aggaattgaa agtaggtttg gaacataacc ggattctgtt
     8101 tttgtatgga gaatatttta tttcctctca ggttttggaa cacaaatttc ccgattacca
     8161 aaaagcattt cctgccgaac tgcccaataa atttgttatt gatagacaag ccctcattac
     8221 tgctataaaa agagtagctc tggtggctcc agatgacaat ttacgcattc attttgattt
     8281 ggatagtgat cgttttgaag tgaataccag taacagagat accggtgaag ctaaagaaaa
     8341 tatggatgac tttacttttg ccggttcttc tacaggaatt tcattcaact ataaatatat
     8401 gctttctatt ctggaagcta tagatacgga aaaagttgtc atcaaactgg gaacttccaa
     8461 agacgcgatg atgatttaca atgaaacacc ccttcccgat caggaaataa ccttcctgct
     8521 gatgcctttg cgttcataaa aatttgaacc tggctaaaat tgaacttgag aatt
//
LOCUS       DAOPFL010000072         8346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_10784_length_8346_cov_105.859607, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000072 DAOPFL010000000
VERSION     DAOPFL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10784_length_8346_cov_105.859607"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLG69_05700"
     CDS             <1..789
                     /locus_tag="PLG69_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43040.1"
                     /translation="IFWVADYRDSWQFGIMIEKLVFPFRLKTIRKTDDKVLATCDAAV
                     FVTPETQLEYINKHSWLKEKSYYIPNGYDEDDFTGITPKKFDKLTLVLMGKLTKVYGS
                     PLNLLKALEDCFPNNYQVIHIGNIDKAILNNIKTSGYTSYNYLGYQKHSDAIAYSLGA
                     DINLIILNENPTAKYWYPGKLFELLRCGKPILALGPKESNLEKILSATKRGRYAYIND
                     KEQIKAQIEYILKNPDQFDTSPETIKQYSREELCKQLLAIYEKG"
     gene            complement(786..1832)
                     /locus_tag="PLG69_05705"
     CDS             complement(786..1832)
                     /locus_tag="PLG69_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPI43041.1"
                     /translation="MKKVTQGKVLVIHTWGIGDLILLTPVLQAVKTLYPLLELEILFF
                     PKSAAIPVTDAPFISKIHFTGWKANLLLPTLFKLRKEKYQAVLFSSGVRPWKTWLFML
                     LLKAEIKVGEYKKFRYPGLSVYVKFNPLLSRTRSNYTLFQSFLNLPSWEEAIVRQNEL
                     NLYPYFHLTEANHQWAENFLAENNLTGKKIIGIHPGCMAKNKRRRWNKEYFIALIEKL
                     KRQYDYPILIIAGPDEIEVGKAIQARTKTLLLLNAPLANVAAIISRLHFFINTDSGLG
                     HIASCFGIKGLTIFGPANELQTAPFSNNSNIIRYPVDCAPCIDKKKKPNCTLDCLLKL
                     TPDMVFKRVQELLD"
     gene            complement(1843..3186)
                     /locus_tag="PLG69_05710"
     CDS             complement(1843..3186)
                     /locus_tag="PLG69_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPI43042.1"
                     /translation="MSQINLKKNIVTSGSFRIVILILSFLISWISARYLGLTLKGEYS
                     YLITLGGFIWMILDWGIFRSYPYLVRKYPDEVKTMFGWTILTMFLELLIFSCLGIVFL
                     DFWSKLIGYPLTPAKIILLIMFISFNKAFMQLQAIYMGMDRILENSLAHFLSSAIFFV
                     LLLIGYFALLEVDRINAVLVFTVAGLFGSVIYLVLRTKWTNWYKQLNFKLVKLFYSYG
                     FRVFLSSLFIMLLIRADIVIIKHFLDFKEVGIYSMAAHIVDFIQIASNLVGGLLFVKL
                     SDIDDDISKWLLLKKTLLLFFFFITLANLGFGLLGKFIMRIMFGVQFVPVYYVYFWLM
                     PAVYGLSFGSLFNNYLNSKGFPIISIILPAVALVINIGLNLWLIPVMGINGAALSSSF
                     SYFLWFILIVYFEQKDSQGKMLKYLLPTRKDIIDLWQETMADLKGLWEKFLHKTA"
     assembly_gap    3271..3370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3559..4707
                     /locus_tag="PLG69_05715"
     CDS             3559..4707
                     /locus_tag="PLG69_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPI43043.1"
                     /translation="MVIAKIVFWISWLLLAYHFVGYGLILFIITSLFKKAKISIPAPD
                     VFPSITVICPAYNEEKVIEEKIRSFLQLNYPKERIKMIIISDDSTDRTNEIVQKYTDQ
                     NISLVIQKPRAGKQNAHNLVLPLLDTDYVLSTDANSIFTPDCVQLLVAKMLSDKRIGL
                     VSGEVKMVKRGSKQSGEAHYWKYEAFLKLMDSRLKTLIGANGPIYLIKRELFSEIPSN
                     SPDDFERVLLTLKRRYIAAYEPQAIIYEEVTEKATEEISRKVRIITQEWCVLKRNIEL
                     LNPFRYPAVSFILFSHKVLRWLFFVFVLTGFVSNAFLLQFGFYRIVFILQVILYLFGT
                     IGLIFQEKGHHIPLTGIPGYFVAMVYSSALAFWNFLQNKKINLWQPVR"
     gene            4717..5709
                     /locus_tag="PLG69_05720"
     CDS             4717..5709
                     /locus_tag="PLG69_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPI43044.1"
                     /translation="MNILITGSNGFVGSNLMRELEEDGHTVIGIDISEHCEGKKHPQT
                     LRGDIRSLKDLNRVASVFLQKQQCPLELIIHCAAAKNDFGVSRQEYYSHNKYGTKTLL
                     NFACEQGIKKLIYISSVGVFGHPEGKANEDFPYNPDTDYGASKLAGELLCIKWQQQNP
                     EHELIVLRPAAIFGPDNFTNTYKLIDTLHRRPYLTIGEGNHIKTIVSIHTVIDMIRFA
                     MQKLAPGYEHYNCIDEPYLTLKELMELICSHPGFQMPRFKIPLKAAIGIGRLFDIPAK
                     LFSIDLPVNSERMRKLGTATYFTAEKAKKDGFVQKMSLKDSITEMCDWYLSINK"
     gene            5729..6757
                     /locus_tag="PLG69_05725"
     CDS             5729..6757
                     /locus_tag="PLG69_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPI43045.1"
                     /translation="MKTVKIGLIGCGRISKNHLDAVSQIPEAEFVAVCDLVEEKMQAV
                     AENYGIKNLYNNYQDMLENEKLDLVSICTPSGLHPQMGIDVAKHKINVLTEKPMATNI
                     ESADALIRACDQNKVKLFVVKQNRLNATMQLLKRAIDKGRFGRIYLAESNVFWQRPQS
                     YYDAEKWRGTWEFDGGAFMNQASHYVDALYWLLGNVDSVMAYTATMARRIEAEDTGCA
                     ILHFRNGIIATINVTMLTYPKNFEGSITIIGEKGTVKIGGVAVNKIEKWEFEDYDDDD
                     RIAQDANYQPPNVYGFGHNPYYRNVIDVLLGKDVPSTDGRDGRKSVEIIQAIYRSAKT
                     GKRVSLPL"
     gene            7581..8303
                     /locus_tag="PLG69_05730"
     CDS             7581..8303
                     /locus_tag="PLG69_05730"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPI43046.1"
                     /translation="MDTREGMPLLGDYFPEMTVVTTKGKMTLPNDMAGKWFVFFSHPA
                     DFTPVCTTEFVAFQKLYPKFKELNTELIGLSVDQVFAHIKWEEWIKENLGVTIQFPII
                     ADTGVVAKSLGLIHPGKGTNTVRAVFIVDAKGIIRIILYYPQELGRNMEEILRAVKGM
                     QFSDANGCSMPANWPKNELIGDKVIIPAAATVADANARIAKIKKGEIEGYDWWFSYRD
                     AKPKQEKKTVTQPKSSSRTKKK"
BASE COUNT         2791 a         1495 c         1495 g         2465 t
ORIGIN      
        1 attttctggg tagccgatta tagagattcc tggcagtttg gcataatgat agaaaaatta
       61 gtatttccct ttcgcttgaa aactatccgt aaaacggatg ataaagttct ggcaacttgt
      121 gatgcagctg tttttgtaac tccggaaacc caattggaat atataaacaa acatagctgg
      181 ttgaaagaaa agtcctatta tattcccaac ggctatgatg aagatgattt tacgggtatt
      241 accccgaaaa agtttgataa gctgactctt gttttaatgg gcaagctaac taaagtttat
      301 ggttctccct taaatttact gaaggcactg gaggactgct ttccgaataa ttatcaggta
      361 atccatatag gcaatataga taaagctatt ttgaacaaca taaaaacctc cggttacacg
      421 agctataatt atcttggcta tcaaaaacat agcgacgcaa ttgcctattc cctgggagca
      481 gacatcaatt tgattatcct aaatgaaaat cccactgcca aatattggta tccaggcaaa
      541 ctttttgaac ttttacgctg tgggaaacct attctggctt taggaccaaa ggaaagcaat
      601 ctggagaaaa tattaagcgc taccaaacga ggcagatacg cctatataaa cgataaagaa
      661 caaataaaag cccaaattga atatatccta aaaaatcccg atcagtttga tacctctccc
      721 gaaacgatta aacaatattc ccgagaagaa ctatgcaaac agttattggc aatatatgaa
      781 aaaggttaat ccagtaattc ctgaacgcgt ttaaatacca tatccggagt aagcttaagc
      841 agacaatcca gtgtgcagtt tggtttcttt ttcttatcta tgcagggagc gcagtcaaca
      901 ggatagcgaa ttatgttact attatttgaa aaaggagctg tctgtaactc gttggcagga
      961 ccaaaaatag ttaacccctt tattccaaag caggaagcga tatgtcctaa accggaatcg
     1021 gtattaataa agaaatgtaa tcgggaaata attgcagcta catttgccag tggagcattg
     1081 agcagaagaa gggtcttagt tcttgcctga atagcttttc ctacttcaat ttcatcagga
     1141 cctgcaataa tcaaaatagg ataatcatat tgccttttaa gcttttctat taaagcaata
     1201 aaatattcct tattccatct tctgcgttta tttttagcca tacaaccagg atgtatgcca
     1261 ataatctttt ttcctgttag attgttttca gccaggaaat tttctgccca ttgatgattt
     1321 gcttccgtta aatgaaaata gggatataaa tttagttcat tttgacgaac aattgcttct
     1381 tcccaggaag gcagatttag gaaactttga aacagagtat agttacttcg ggttctggaa
     1441 agaagaggat taaatttaac atagacggat aaacccggat agcgaaactt tttatattct
     1501 ccaaccttga tttctgcctt taagagtaac ataaaaagcc aggttttcca ggggcgaact
     1561 ccggaagaaa aaaggacagc ttgatacttc tctttccgta gtttgaaaag agtaggaagc
     1621 agtaaatttg ctttccagcc agtaaaatga attttactaa taaaaggagc atctgttaca
     1681 ggtattgcag cggacttcgg gaaaaaaaga atttccagtt ccagcaaagg atataaggtc
     1741 tttactgctt gtaaaacagg agttaataaa atcagatcac caattcccca agtatgaata
     1801 actaaaactt ttccctgtgt aacttttttc attgtaaaga ccttaagccg ttttatgcaa
     1861 gaatttttcc caaagacctt tcaaatcagc cattgtttcc tgccataaat caataatatc
     1921 ttttctcgtt ggtaagaggt attttagcat tttgccctgg ctgtcctttt gttcaaaata
     1981 aacaattaaa ataaaccaga gaaaatagga aaaactactg ctgagtgcag ctccgttgat
     2041 tcccataacg ggaatcagcc aaagatttaa gccaatatta attactaaag ccacagcggg
     2101 aaggataata ctgattatag gaaagccctt actattcaag taattattaa acagcgaacc
     2161 aaaacttaaa ccgtaaacag ccggcattaa ccaaaaataa acataataca caggcacaaa
     2221 ttgaactcca aacattatgc gcattatgaa tttgcccaga agaccaaaac ccaaatttgc
     2281 caaagttata aagaaaaaga acaacagaag ggtcttcttc agaagcagcc atttactaat
     2341 atcatcatct atatcggaaa gctttacaaa caataaaccc cctacaaggt ttgaagcaat
     2401 ctgaataaaa tctacaatat gtgctgccat agaataaatt cctacttcct tgaaatccaa
     2461 aaaatgcttg attattacaa tatcggctct aattagtaac ataatgaaca gagaagaaag
     2521 aaaaacacga aaaccataac tgtaaaacag cttaactaac ttgaaattta actgcttata
     2581 ccaattagtc catttggttc gcaataccaa ataaataaca ctgccaaata aacctgcaac
     2641 ggtaaaaacc aagaccgcat taattctatc tacttctaac agagcaaaat aaccaataag
     2701 caaaagaaca aaaaagatgg cggaacttaa aaaatgcgcc aaagaatttt ccagaattct
     2761 atccattccc atatatattg cctgtaattg cataaatgcc ttattaaaag aaataaacat
     2821 tattaaaagg attatttttg ccggagtaag aggataacca atcaatttac tccaaaaatc
     2881 cagaaaaaca atgcccagac agctgaaaat gagcaattcc aaaaacatcg taagaatagt
     2941 ccagccaaac atcgttttca cttcatcagg atatttacgc actaaataag gatagcttct
     3001 gaaaattccc caatccaaaa tcatccagat aaaacctcct aaagtaatta aatagctgta
     3061 ttcacctttc aaagttaaac ctaaatacct ggcacttatc cagcttatta aaaaggaaag
     3121 aattaggatt actatcctga aactgccact ggtaacgata tttttcttca ggtttatctg
     3181 ggacattttt ctctgttttt gctgtgtaaa ttttggtttt tactataatt cataatgcca
     3241 taatgcttat atcgtccttt ttgtcaaatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn ataaaaataa cctcggtgtt ctatgtattt tcggcgtaaa ccaaaattaa
     3421 aatcataact ctatgttttt ttattgtgag gtgtaagtgg acttaatttc cttgacagtt
     3481 aacagcttga ttttatcagg aactttcaag gtaaatgcaa tgttaacttt atataagaca
     3541 caaagaaaag gacaataaat ggtaatcgcg aagatagtat tctggatttc ctggctactt
     3601 ttagcttacc actttgttgg ttatggatta atcttattta tcataacttc actgtttaaa
     3661 aaagcaaaga tttcaattcc cgcaccggat gtctttcctt ccatcactgt aatatgtcct
     3721 gcctacaacg aggaaaaggt tatagaagaa aaaatccgtt cctttttaca gctgaattat
     3781 cctaaagaaa gaataaaaat gattattatt tctgatgatt ccaccgatag aactaatgaa
     3841 atagtgcaaa aatataccga tcaaaacatt tctctggtaa ttcaaaagcc aagagcaggc
     3901 aaacaaaatg ctcataatct tgtgttaccc ttactggata ccgattatgt tttatctacc
     3961 gatgccaaca gtatttttac tcccgattgt gttcaacttt tagtagctaa aatgctttct
     4021 gataaaagga taggtctcgt ttcgggagaa gtgaaaatgg ttaaaagagg cagtaaacaa
     4081 tcgggggaag ctcactattg gaaatacgag gcatttttaa aattgatgga ttcacggcta
     4141 aaaactttaa tcggagctaa tggtcctatc tatttgatta aaagagaact tttttcggaa
     4201 attccctcaa acagtcctga tgattttgaa cgggttttac ttactttaaa acgaagatat
     4261 atagctgctt atgaaccaca agccatcatt tatgaagaag ttaccgaaaa agcgacggaa
     4321 gaaatatccc gaaaagtgag gattattacc caggaatggt gtgttttgaa acgcaatatt
     4381 gagttattaa atccgtttcg ttatcccgct gtaagtttta ttttattttc gcacaaagtg
     4441 ttacgctggt tattctttgt ttttgtattg acaggatttg tcagtaacgc ttttttgttg
     4501 cagtttggtt tttacaggat tgtttttatt ttgcaggtga tattatattt gtttggaact
     4561 ataggactta tattccagga aaaaggtcac catattcctt taaccggaat tccgggatat
     4621 tttgtagcga tggtctattc ctcagctctt gctttttgga acttcctgca aaataagaag
     4681 attaatttat ggcaaccggt gcgctaagga gaaaaaatga atattctaat taccggcagt
     4741 aacggttttg tcggttccaa tttaatgcgg gaactggaag aagacgggca tactgtaatc
     4801 ggtattgata tcagtgaaca ttgcgaaggg aaaaagcatc cccaaacgct gagaggagat
     4861 atcaggtcgt taaaggattt aaacagggtt gcctctgttt ttttacaaaa acagcaatgt
     4921 ccactggaat tgattattca ttgcgcagca gcgaagaatg actttggcgt ttcccgtcag
     4981 gaatattata gtcataataa atacggcacc aaaacattac tgaattttgc ctgtgagcaa
     5041 ggcatcaaga agttaattta tatcagttcc gttggcgttt ttggtcatcc ggaaggtaaa
     5101 gcaaatgagg attttcctta taatccggat actgattatg gcgcttcaaa actggcagga
     5161 gagttgcttt gtattaagtg gcagcagcaa aatccggaac acgaacttat tgttttgcgt
     5221 ccggcagcta tttttggtcc cgataatttt accaatactt ataaacttat agatacttta
     5281 catagaagac cctatttaac tatcggcgaa ggaaatcaca ttaaaaccat cgtttctatc
     5341 catactgtga ttgatatgat tcgttttgca atgcagaaat tagcccctgg atacgaacat
     5401 tataactgca ttgatgagcc atatttaacc ttgaaggaat taatggagct tatttgttcg
     5461 catccaggtt tccaaatgcc ccgctttaaa atccccttga aagcagcaat tggcattgga
     5521 agattgtttg atattccagc gaaacttttt tctatagacc ttccagtaaa tagtgagagg
     5581 atgcgtaaat taggaaccgc tacctatttt actgccgaaa aagctaaaaa agatggtttt
     5641 gtacaaaaga tgtccctaaa agatagcatc accgagatgt gcgattggta tctttctata
     5701 aacaaataac ttaacaggag tattttggat gaaaacagtg aaaatcggct taattggctg
     5761 tggccgcatt tctaaaaatc accttgatgc cgtttctcaa attccggaag cggagtttgt
     5821 tgccgtttgt gaccttgtgg aagaaaaaat gcaggcggta gcagaaaatt atgggattaa
     5881 aaatctttat aacaactatc aggatatgct ggaaaacgaa aagttagacc tcgtttcaat
     5941 ttgcacacca agtggcttgc atcctcaaat gggaattgat gtagcaaaac ataaaatcaa
     6001 tgtgttaacc gaaaaaccaa tggctacaaa cattgagtca gccgatgctt taatccgggc
     6061 ttgcgaccaa aataaggtga aactttttgt agttaaacaa aatcgcctca atgccactat
     6121 gcaactcctt aaaagagcta tagataaagg acgctttggc agaatctatt tagcggagtc
     6181 caatgttttt tggcaaagac ctcagtccta ttacgatgcc gaaaaatggc gtggaacctg
     6241 ggaatttgat ggcggtgcct ttatgaatca ggcaagtcat tatgtggatg cactttattg
     6301 gcttttagga aatgtagaca gcgttatggc ttacacagca actatggctc gccgaattga
     6361 agcggaagat acgggttgtg ctatcttaca ttttcgcaat ggcataatag caactatcaa
     6421 tgttacaatg ttaacttatc ccaaaaattt tgagggctct atcactatca tcggggaaaa
     6481 aggaaccgtt aaaatcgggg gtgtggctgt aaataaaata gaaaaatggg aatttgagga
     6541 ttacgatgat gatgatcgca ttgctcagga tgccaattat cagccgccaa atgtttatgg
     6601 ctttggtcat aatccttact atcgcaatgt gatagatgtc cttttaggca aagatgtccc
     6661 ttccaccgat ggaagagacg gcagaaaatc agtggaaatt attcaggcaa tttaccgttc
     6721 tgccaaaacc ggaaagcgcg tttctctacc tttataaagt aaggattttt ttccccttag
     6781 ggcagcaaaa tcatttagca tacaatttca gaccttgggc tgccgaattt caattcggaa
     6841 aagggaatac cgaaaatgtt ttgggaaaac tataggatat catttctatt ttcataatcc
     6901 atttgtcatt cctgcaaaaa ctaaaatcca gatattttta atctatttaa ctgaattttt
     6961 aatattgaac atattatctg tgccaaattg gaatttacca ccctttttcc cctcaactat
     7021 gtttttctgc ttcatctttt tttgcctgca taaccatcta ataatccagt aacaatcaag
     7081 taacaattcc gtaacagaac tacggaagtg ttacggaagt gttacggtgt tgttacttaa
     7141 tcaactatat aacaaacaat tacacatctt aagttaaaaa acagtttaca acgggtttca
     7201 accaggcgat aaacaagcaa aagaaggaga aaaaattccc ggcaggaaga cagataaaga
     7261 aaatagagat aaaaaatctt tcggaaaaaa agcaagatga attatcagac aagaaacttg
     7321 gaaattattc taaaaatacc agttcggtca ttttaacgga acttttttta gctaagaatc
     7381 tccatttttt gcctggttca aaacccgtcg ttatttttct gctcttccta cggaattttt
     7441 tctgctattt acatattatg aatatggaga tagtatgaaa aattcagcaa aacgattacc
     7501 cggttctcaa aaaaatattt gttttttctt ctaattgctc tattatatat tgtaacgcaa
     7561 ataatgcaaa ggagaaaaaa atggatacaa gagaaggtat gcccttgctg ggggattatt
     7621 tccccgaaat gaccgtcgtt acaactaaag gtaaaatgac ccttcctaat gatatggcag
     7681 gaaagtggtt cgtttttttt agtcatccgg ctgattttac ccctgtttgc actacggaat
     7741 ttgtcgcctt tcaaaaactt tatcccaaat tcaaagagct caatactgaa cttatcggat
     7801 taagtgtaga tcaggttttt gcacatatta aatgggaaga atggattaaa gaaaatcttg
     7861 gtgttaccat tcaatttccc ataattgccg ataccggtgt tgtagcaaaa agcttgggac
     7921 ttattcatcc cgggaaagga acaaatactg tccgtgctgt atttatcgtt gatgccaaag
     7981 gtatcattag aattatcctc tattatcccc aagaactggg acgcaatatg gaagaaattc
     8041 tgcgtgccgt taaaggaatg caattttcag atgctaacgg ttgctctatg cctgcaaact
     8101 ggcctaaaaa tgaattgatt ggagacaaag tgattattcc ggctgctgct actgttgctg
     8161 atgccaatgc ccgcattgct aaaattaaaa aaggggaaat tgagggctat gactggtggt
     8221 tttcttaccg ggatgccaaa cctaaacagg agaaaaaaac ggtaacacaa ccaaaatctt
     8281 cttcccgcac aaaaaagaaa tgaactaaag aggcagttga aaaactgcct ctttttttaa
     8341 ccccca
//
LOCUS       DAOPFL010000073         8339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_10789_length_8339_cov_110.810961, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000073 DAOPFL010000000
VERSION     DAOPFL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10789_length_8339_cov_110.810961"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLG69_05735"
     CDS             complement(<1..619)
                     /locus_tag="PLG69_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPI43047.1"
                     /translation="MIQNLLLIRNQAQLKEMGSPLKYNILKELIKAPATCQQLATLFG
                     CSKQKMHYNLTQMLSQGLLKVEDEANINNKEVYYRATARSYVLDLAIGLESSEKILDN
                     RNIINSILEQEYRINLNNIADKLLDEALKLTKGMHLLITTGKFNLPLVEKLLVEAGKR
                     GIYTTLIYQDLEQLQARAEQYSLIAFQADYENFNHKLKEADVYLNL"
     gene            complement(1183..1512)
                     /locus_tag="PLG69_05740"
     CDS             complement(1183..1512)
                     /locus_tag="PLG69_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43048.1"
                     /translation="MKTMQIIILFLIILELGFLCYLGEKMNRGNNVTSEKPPLEAFTN
                     TLHQLNENALEIIGWQTTPYGCLVIYKGFGWGKEYYEEEQFVKVQSEYGTDWYMKDGL
                     RYYKVQN"
     gene            2427..2502
                     /locus_tag="PLG69_05745"
     tRNA            2427..2502
                     /locus_tag="PLG69_05745"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2460..2462,aa:Ala,seq:cgc)
     gene            2696..2845
                     /locus_tag="PLG69_05750"
     CDS             2696..2845
                     /locus_tag="PLG69_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43049.1"
                     /translation="MKLERPQLHSAIIKRLGNITFWRSKINFRDKMEEIYTRVGKKAE
                     NILEQ"
     gene            3322..6519
                     /locus_tag="PLG69_05755"
     CDS             3322..6519
                     /locus_tag="PLG69_05755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPI43050.1"
                     /translation="MARLFAKDYHNKSDSWYFNQAIIVLEKDDLWFWKTRNEDGNYQI
                     RFFPAPSKQEYFAEYVDIVFNPETELIVSHDCSQCHTDESCRHYLSILRYAYLNLSTR
                     IFEEEVVETCDGNALSGNELWLNIARNAVLYVEGIYNPETDKIRFYHQDLAPIDPILM
                     GKLYSQEEITDIPQRQIEYYTANMGAFRDADLHLFSFLNKKRCAYSHKNKFWSIYKND
                     FAQALLIMQNCHTRFVIRETGEELIFQQQPYQLALRIEPSGTKNYSLRAVIIDELSVW
                     FAGNPTWLFFRNRVYKINLPFRKEIIDHIFSSGEVLTARDLIYYRCLVHRELQEQGIY
                     LDFEESISLPEIIDETPVKRLHIKKLGDKILIGGSLVFSQEREIPLSVLRFGKPLVYS
                     QYTSPDKNGSAWFHLTPELFAKTQELLNKLPEPEMNRLEQYAELVFSGKERIEQLRTA
                     IFELSDQDWDIQIDPELGNYFVAKIPLQVELIARHEEDIDWFTYEVRYHYRDLNFTHE
                     ELKRFFRSKEEFLHTSDGRLVFISNPQVFYEVEELLSRSEHKTDQVYRSRMMNLPYYL
                     KLREDNPAFRMQGDEWLEGFYEALLKRRLEGPETLPLYLQTVLRGYQKAGVAWIKMLA
                     HYHLNGILADEMGLGKTIQALSAILSTTLGQVSLVICPKTLLYNWAAEIDKFHTNIPF
                     AIVDGNKTTRMEILSNPNVQLFIMSYSMVLGDVAYLKNMEFEWIVLDEAQNIKNVSAQ
                     RTSAIKKLKSKHRLALTGTPIENNLTELWSIFDFLNPGYLGTLNKFKQNYLPAEGEIT
                     ARLSLSRMVAPFMLRRIKKDVLLELPDKQEQISWCKLTTLQEKLYLQILDMVHKKLLP
                     EGKEMPSYIHILSALTKLRQVCNHPHLANGDILPELEASSKLEQLLELVTEATNAGHK
                     VLIFSQFVQMLSIIRKVLEANSLPYCYLDGQTKDRVTPIKSFETNPEIKLFLISLKAG
                     GTGLNLTAADTVILYDPWWNPMVENQAIDRTHRIGQTHKVQVFRLITKGTVEEKILQL
                     QQNKRELFETVIEGGQNVLKAMTKEDIKKLFSYSE"
     gene            complement(6947..8023)
                     /locus_tag="PLG69_05760"
     CDS             complement(6947..8023)
                     /locus_tag="PLG69_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HPI43051.1"
                     /translation="MDFKDEIKQIADKIEMQKDQIQTEEATKNAFILPFIKALGYDIF
                     NPFEVIPEYVCDIGTKKGEKIDYAILKENKPIILIECKHWKSELNLYESQLLRYFHVS
                     KAKFGILTSGIIYRFYTDLKEPNKMDEKPFFEINLTDIRENQIEELKKFHKSYFDIDN
                     ILSTASELQYTNELKNMIHNEIINPSEPMVRLLAKNIYSKPLTSKIIEQFTELVKKSV
                     NSVISDIITERLKTVLIKEKEAETDKKKEEKDNIIKDDGINTTIEEIEAFYIVKAIVS
                     KRVALSRITYKDTKSYFVIMLDDKIQKPICRLEIGKNTIILKTFDEDKYGKRHKLNSL
                     EQIYDYSKRLLDTVDKYESSNNKK"
BASE COUNT         2487 a         1593 c         1431 g         2828 t
ORIGIN      
        1 taaggttcaa ataaacatcc gcttctttca gtttatggtt aaaattttca taatccgcct
       61 gaaaagcaat taacgaatac tgctccgccc ttgcttgcaa ttgttctaag tcctgataaa
      121 tcaaagtagt ataaattcct cttttccctg cctccactaa taatttctcc actaaaggca
      181 aattaaactt gcctgttgta atcagcaaat gcattccctt cgtcagtttc agcgcctcat
      241 caagcagttt atcagcaata ttattcaaat ttatccgata ttcctgttct aaaatgctgt
      301 taataatatt gcggttatcc aatatttttt cactgctttc caaaccaatt gccaaatcca
      361 aaacataact gcgggcagta gctctataat agacctcttt gttattgata tttgcctcgt
      421 cttcaacttt tagtaaacct tgagaaagca tttgcgttaa attatagtgc atcttttgtt
      481 tactgcaacc aaacagagtt gccagttgct gacaggttgc cggtgcttta atcaattctt
      541 ttagaatatt atattttaag gggctgccca tctccttcag ctgggcttga ttcctgatta
      601 gcagaagatt ttgaatcatt ttttcctcct tcctaacatt taattttaca taaagtacac
      661 tctcctgtta ccctaatatt tagtcaagaa ctttttgact atggtcacga aatctaagga
      721 aataattctg ctatagagta tcaagtgaaa ttgcaaatag tatcactttc gttgcaaatc
      781 gccgtttttg gtgagacccg agtctcaacc tttttgcaaa tccacaatgt tgacatcctt
      841 atattgatag atttatcata ataaaaaaat gggaaacacc ggcagcttcc tatgtttcgg
      901 agagcattct atgtttccat gtttttcttt gcctggaatt atgatctatc tgaagataga
      961 taactggatt gccactgacc gatttgcaaa aaacggtgat cagattttca gaaatggtga
     1021 tcagaatcat ttgcaaaaac agcgatcaga attagctccc aaaatgccat ttgcaatttt
     1081 cagtgatcag atttgcattt tttaatgatc aaatacacct gaacatctgg atagggcaac
     1141 tccttgaatt agaagcattc ctcattaaca atgatgatct ttctaattct gtaccttata
     1201 gtagcgtaaa ccatctttca tataccaatc agtaccatac tcagattgta cttttacaaa
     1261 ttgctcttct tcgtaatatt ccttacccca gccgaaccct ttatagataa ctaaacaacc
     1321 gtaaggtgtt gtttgccatc caattatttc aagtgcattt tcattcaatt ggtggagagt
     1381 attggtaaaa gcttctagag gtggtttttc actggtgacg ttattccccc tattcatctt
     1441 ctcacctaaa taacagagaa atcccaattc cagaataata agaaataaga tgattatttg
     1501 catcgtcttc attgtaggtc tcctttgtga ctgtaatata tctatagtcg catttaactt
     1561 aataatcttt ttaatttatc ctattcattt cgtatgtcaa tggtctatta tttcatagaa
     1621 tatagaagta ttaaatttac cattacactt tctgtcaaga tattttctac ctccttcaaa
     1681 actatctact ttatgcgttc tcacttattt tttcaaatcg tatcaccaat ttttccaatc
     1741 ggtgttttac tgacctgcga tatcacgatt ttttcaaaac gatatcataa ttttttcaaa
     1801 tggatttcgc ataacattgt atgacttgcg attattttag gcgcagcttc aatctgattg
     1861 tttatagagg catctatctt tcttatcgta ttgagcggat aggacttgac agagttattt
     1921 cctcattttt tacgaggggc ttacgccacc atcgctatta ttgtttcgcc cttcgggctt
     1981 tttggcaaag ggcaaagagc taagaacaga gggactctaa accctctcaa cgctctcaac
     2041 cttttcaacc ttctaaacca ttttttacga ggggcttacg ccaccatcta cattattgtg
     2101 tcgcccttcg ggcttttgta aaaaggaaag gaaaaagaaa gaagtcgggg ctttcatttt
     2161 aacgaggggc ttacgccacc atcgctataa ttgtttcgcc ctacgggctt ttaatgtatc
     2221 tcaaccttct aaaccctcta agccttctca acccttttta acgaggggtt tacgccacca
     2281 tcgctatcgt tgtgtcgctc tccgagctgt taaagaattc tcaaccctct caaccttctc
     2341 aaccctctca atttttcatt tttccttctt cctcacattg ctcttgacaa aaccctatct
     2401 cttaaaattt attcccaaag tgcgttgggg ctatagctca gttggtagag cgaatggttc
     2461 gcaatcatta ggccaggggt tcgaatcccc ttagctccac cacaaatttt atttcttacg
     2521 gtaagcgaga gtggttatat tacatatatt cattatcaat taagttccgt ttctctgaag
     2581 cgaaagaata ctgtccactt acgaaagaac aaattaccct ttataaagca gtagcggatg
     2641 atttgccaaa taaagaagga attgaaagaa agggacttat tttaactgca gagaaatgaa
     2701 actggagcgt cctcaactcc attctgcaat tattaaacgc ttgggaaata ttactttctg
     2761 gcgttctaaa atcaatttta gggacaaaat ggaggagatt tataccagag ttgggaaaaa
     2821 agcagaaaac atattagaac aataagttac ataatttttt gtcctttttc cctcaatttt
     2881 tataactctc tttcttgcct ccattacctt agcattgctt acccaatact aatgccatcc
     2941 gtaatgggta agcaatgagt aagcaatgct attaaggacg aaatcggaat tttctctttt
     3001 ccaactaatt acccacacgc agaagaaaaa ataatgaggg tgcgattttc ctttcccttt
     3061 tgggggatta acgatgtctt attttcccta aattacaaga acatatctgt gttatctgtg
     3121 caatctatga gagattaatt tcattaattt ctgtggtgaa gacatctccc atctcctctt
     3181 ttctctttat ttacaaatca ttcttggtgt tttcagtgct ttctgtgctc tcgctgtaaa
     3241 aaaaacccct tttttagatt gacagatttt ccctttttac cctataggaa ctgattaaaa
     3301 aataaaagga tacccaaact gatggcacgg cttttcgcta aggattatca taacaaatca
     3361 gacagctggt atttcaatca ggcaataatt gtgttggaga aagacgattt atggttttgg
     3421 aaaacccgca atgaagatgg caactatcaa attcgttttt tcccagctcc ttccaaacag
     3481 gaatattttg ccgaatatgt agatattgtt tttaatccgg aaacggagct gattgtttcc
     3541 cacgattgtt cacagtgcca tacggatgaa tcctgtcgtc attatctttc cattttgcgt
     3601 tatgcttatc tgaatttaag cacccgcatt tttgaggaag aggtagttga gacctgtgat
     3661 ggtaatgccc tcagtggcaa tgaattatgg ctaaatattg cccgcaatgc agttctttat
     3721 gtggagggaa tttataatcc cgaaaccgat aaaatccgtt tttatcatca agaccttgct
     3781 cctattgatc caattttaat gggtaaactc tattctcagg aagaaataac cgatattcct
     3841 caacgccaaa ttgaatatta tactgccaat atgggagctt ttagagatgc cgaccttcat
     3901 ttattcagtt ttttaaacaa aaaacgctgt gcttatagtc acaaaaacaa gttctggtca
     3961 atttacaaaa atgactttgc ccaagctctt ttaattatgc aaaattgtca tacccgtttt
     4021 gtcattagag aaacgggtga agagcttatt tttcagcaac agccctatca acttgcctta
     4081 agaattgaac cctccggcac aaagaactat tctttacgcg ctgttattat agatgaactc
     4141 tccgtttggt ttgccggaaa tcctacctgg ctttttttcc ggaatcgggt gtataaaatt
     4201 aacctgcctt tccgaaaaga aattatagat catattttta gcagtggaga ggttttaact
     4261 gccagggatt tgatttatta tcgctgtctg gtgcatcgtg aactgcagga acagggaatt
     4321 tatctggatt ttgaggaaag tatatcgctt ccggaaataa tagatgaaac ccctgttaag
     4381 cgtttgcata tcaagaaatt gggtgataaa attctgattg gcggttccct ggttttttcc
     4441 caagaaagag aaattcccct ttccgttttg cgttttggta agccccttgt ttattctcaa
     4501 tatacttctc ctgataaaaa tggtagtgcc tggtttcatt taaccccgga gctttttgct
     4561 aaaacacagg aactgctgaa taaacttccc gaaccggaaa tgaaccgttt ggaacaatat
     4621 gccgaattgg tttttagcgg aaaagaaaga attgaacaat tgcggactgc tatttttgaa
     4681 cttagtgatc aggattggga tattcaaatt gacccggaat tggggaatta ctttgtggct
     4741 aaaattcctt tgcaggtgga gcttattgct cgtcacgaag aagatattga ctggtttacc
     4801 tatgaagtcc gttaccatta tcgggatttg aattttacgc acgaagaact gaaacgcttt
     4861 ttccgcagta aagaggaatt tttgcatacc tcggatggac gccttgtgtt tatcagtaat
     4921 ccgcaggtct tttatgaagt ggaagaactc ctttcccgca gtgaacataa aactgatcag
     4981 gtgtatcgtt cccgaatgat gaacctgccc tattatctta aattgcggga agataatcct
     5041 gcctttagaa tgcagggtga tgaatggtta gaaggatttt atgaggcgtt actaaaaagg
     5101 cgattggaag gtcctgaaac cttacctctc tatttgcaaa ccgttttacg cggttatcaa
     5161 aaagccggtg ttgcctggat taagatgttg gcacattatc acttaaatgg tattcttgcc
     5221 gatgaaatgg gcttgggaaa aaccattcag gcactttctg ccattttatc tacaactttg
     5281 gggcaggtct cactggtaat ttgtcctaag accttgcttt ataattgggc tgcggaaatt
     5341 gacaagttcc atactaatat cccttttgcc attgtggatg gtaataaaac gacccggatg
     5401 gaaatcctct ccaatcctaa tgtccagctc tttattatga gctattctat ggttttggga
     5461 gatgttgctt acctgaaaaa tatggagttt gaatggattg ttctggatga agcacaaaat
     5521 attaaaaatg tttccgctca acgcacttcc gccatcaaaa aactgaaaag caaacaccgt
     5581 ttggctttaa cgggaactcc tattgaaaat aatcttaccg aactttggtc tatatttgat
     5641 ttcctgaacc ctggctattt gggcaccctg aataaattta aacagaatta tctgcctgct
     5701 gaaggagaaa taactgcccg actttcttta tcccgaatgg ttgccccttt tatgctgcgc
     5761 agaataaaaa aagatgtgct cctggaactt ccggataaac aggaacaaat ttcctggtgc
     5821 aaactaacta ccctgcagga aaaactttac ctgcaaattt tggatatggt gcataaaaaa
     5881 ctcctgccgg agggaaaaga aatgccaagc tatattcata ttttatctgc acttacaaaa
     5941 ctaagacagg tctgcaatca ccctcatctt gccaatggcg atattttacc ggaactggaa
     6001 gcatcctcca aactggaaca gcttctggaa ctggttacgg aagcgacaaa tgcaggacat
     6061 aaggtcttga ttttcagtca gtttgtccaa atgctgtcca ttattcggaa ggtcttggaa
     6121 gcaaattcct tgccctattg ctatctggat ggccaaacca aggatagagt tactcccata
     6181 aaaagctttg aaaccaatcc cgaaattaaa ctctttttaa tctccttgaa agcaggtgga
     6241 acgggactta acttaactgc tgcagatacc gttatcctat atgatccctg gtggaatcca
     6301 atggtggaaa atcaggcaat agatagaact cacagaatcg gacaaacaca taaggttcaa
     6361 gtttttcgtc tgattaccaa aggcactgtg gaagaaaaaa tcctgcaact gcaacaaaac
     6421 aaacgcgaac tctttgaaac ggttattgag ggtggacaaa atgtgctgaa ggcaatgacg
     6481 aaagaagata taaagaaact ttttagctat tcggaatagg gtcaagaggt caagagatca
     6541 ataggtctgg aggaaagtga aaaggggaaa aggggaaacc caaggcaagc atcgtaaaat
     6601 atttatgtca tttcttgaat aaaaagtcat ttgacattcc tgcaaaagca ggaatacaca
     6661 taatataaga aaatattatc ccttttcaat atgaaaatta tattagatag aaagatagat
     6721 aacaaagaaa gtctgtagcc attgtgtagt tggaacttgt tgaagttctg cattttgtct
     6781 gttagaactt tttagtgtag gtttttttga ggaggaacag cgtcctccgt ctacacatat
     6841 tatcgtcacg attccgtact gacaaacaaa tatgtaaaaa tattaaaagt tattgagaca
     6901 cagacgactc ctgacttaca ttgaaattaa attattatca atcactttac ttcttattat
     6961 tactactttc gtatttatca accgtatcaa gtagccgttt agaataatca tatatttgtt
     7021 ctaatgaatt aagtttatgc cttttcccat acttgtcttc atcaaaagtt tttaatatta
     7081 ttgtattttt acctatttct aatctacaaa taggtttttg gattttgtca tctagcataa
     7141 ttacaaaata tgacttagta tctttatagg taattctact tagagcaact cgcttagaaa
     7201 caatggcttt aactatatag aatgcttcta tctcttcaat tgttgtattt atcccatcat
     7261 ctttaattat attgtctttt tcttcctttt tcttatcagt ttctgcttcc ttttctttaa
     7321 ttaggacagt ttttaatctt tctgtaatga tatcactaat aactgaattc actgattttt
     7381 taacaagctc agtgaattgt tcaatgatct tgcttgttag aggtttagaa taaatatttt
     7441 ttgctaacaa acgaaccatt ggttcacttg gattaattat ttcattatga atcatattct
     7501 tgagttcatt tgtatattgc aattcactag cagtgctaag aatattatca atgtcaaaat
     7561 aggatttatg gaattttttt aattcttcaa tttgattttc tctaatatct gtaaggttta
     7621 tctcaaaaaa tggcttttcg tccattttat ttggttcttt caaatcagta taaaagcgat
     7681 aaataattcc acttgtaaga atgccaaact ttgcttttga cacatgaaaa tatctaagta
     7741 gttggctttc gtaaaggttt aactcacttt tccaatgttt acattcaata aggataatag
     7801 gtttattctc ttttaagatg gcatagtcaa ttttttctcc tttttttgtc cctatatcac
     7861 atacatattc cggaataact tcaaaagggt taaatatatc atatcctaat gccttaataa
     7921 atggaagtat aaaggcattt tttgtagctt cttccgtttg aatttgatct ttctgcattt
     7981 caattttgtc agctatttgc ttaatctcat ccttgaaatc cattggaacc tccaataact
     8041 ttttttcatc ctttttatct tgaaagattg tgtcaactaa aatattattt ctattggctt
     8101 ttctttctct ttagcttaca tattaccgtg aatataacac ataaaattca aatttaattg
     8161 tcttaatgat gtgtagtcgg aatttgctga atctctgcat tttgtctgac agaatttttt
     8221 tattgtagtt tttttttacg gaggaacagc gtccttggtc tacacatttt atcgtaaaaa
     8281 aggtgaaacc tgaaaaataa atagagaggg aatagtaata ttcttctttt cccgagggg
//
LOCUS       DAOPFL010000074         8307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_10855_length_8307_cov_108.250848, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000074 DAOPFL010000000
VERSION     DAOPFL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10855_length_8307_cov_108.250848"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1168)
                     /gene="mraY"
                     /locus_tag="PLG69_05765"
     CDS             complement(65..1168)
                     /gene="mraY"
                     /locus_tag="PLG69_05765"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPI43052.1"
                     /translation="MFYHLLYPLAKYSIVFNVFRYVTFRSIGAFITSLIISLLVGPAF
                     IRMLKKKSAVETIDEDLPERHRLKQGTPTMGGIIILVSLLISSLLWNVLTNPAILMII
                     LTTVWLGILGFLDDYLKNYVKAKKGLIPRYKLLGQISVGLILTLAIYYSNSFSKNITA
                     MQIPFFKNLIVPLGWVFIPFAVLFITGTSNAVNLTDGLDGLAAGTLIFSFLGLGVMSY
                     LKGNYIAANYLNLEFISSAGELTVFISAIMGTLMGFLWFNSYPAEVFMGDTGSLTLGG
                     IAAVISILLKEEIFFFILGFIFVYEALSVIIQRSWFKYTKRKYGEGHRVFLCAPVHHH
                     YELKGIHESKIVMRFYIVAALLTAVALSTIKLR"
     gene            complement(1168..4017)
                     /locus_tag="PLG69_05770"
     CDS             complement(1168..4017)
                     /locus_tag="PLG69_05770"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPI43053.1"
                     /translation="MINRILYALQQHSLLVETANLAKLENWQGKVQTDNRKIKPGDIF
                     VCIQGANFDGHNFINSALEQGADIIVCREINKRDIPYIKVKDTRKAAAVIARTVMLPE
                     KIPYTLIGITGTNGKTTTSLLLYEALRNLGFRCGWIGTLGYYIEGKAFPSERTTPDII
                     ELNEIFGQMIEAKVDYVVMEVSSHSLSLDRIYGVKFDYCLFSNLGRDHLDFHQTLENY
                     GKAKMSFFEQGAKDGSISVINTADAFGVQIKDMLKEKQAKVYSLGGNDAEFQYSDIKT
                     DINTSSFKLIAENCEIPVTSRLIGSFNIQNLGLTALTLYALGFSPEAIHNSLKDIPPV
                     QGRLERVENNFNIGIFIDYAHTPEAIDNVLKAVEDLPHKRILCLIGAGGDRDKGKRPL
                     MLKSALNHSDVVIISDDNPRSENPETIIKDIVSSSDIFLPWWIIRNRQQAISSIIRLA
                     QEEDIVLICGKGSEDYQEIEGVRHHFNDREETLKALAEKEIKDKADDELILPIDQTLL
                     KLLLLPNWEPEPKGYQETYSYYYLSIDSRSIKPGSVFIAIKGEKYDGHSYLGDVLKIK
                     ENCAIGEIPYPLNQFSEENSYYQVENCLETMGLICRKYLQLFPAKRIALTGSTGKTTC
                     KELMAQVLQSSAPVLKTLANENNIIGLCKTIIRIKPEQQYAIFELGTNHFGEIAALAD
                     VANPDIGIILNIGPSHLQYFGDEEGVFREKITLFKRPLAFRLYPGDDFRFDYLTNEGI
                     SIGYAENCDYRITRHKCNKDNQSFYLNEDLWFLPYGAPHYAINSAFAIVLGLKLGIDK
                     EKMQTALNKPIQLELRSQVEKRGTGLLILDCYNANPYSMQKALEYWQELNPEQPHYAI
                     LGDMLELGESSILYHQMISAMLAEMRYEKLFTVGNYSRIYHPEKESPIFHYNTVEELI
                     ADRKLNSLPENAIILVKGSHAIQLERVIPILKGED"
     gene            complement(4014..6212)
                     /locus_tag="PLG69_05775"
     CDS             complement(4014..6212)
                     /locus_tag="PLG69_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPI43054.1"
                     /translation="MCKFCWTKMKSRYYFLVVIFALVAVIWIAYLFCIQILDPFHLAG
                     ARRMRYTPQKEILIPRRGSILDAQGNLLVSSVIYYQIDIDRSSVASWAKEEKIPLEKA
                     FEKYAEVISSHTTLNKDDVIKRLNYGNKTSSIQISNKISEVELDKLIKELKTKDMPGL
                     VHSFASMKRIYSKDILAARVLGSVREVSDGYDPATNSKSLYKLAGVCGIESTYDKYLS
                     GNYGWKEVVLDANHQPVPYPNLHSKKPENGYNLYLTIDAKIQEIVENALYEGMENYGA
                     KNAGAIVMDPNTGKILAMAGVSGEDKTEDPNFVRVKSNIPVSFMFEPGSTMKPLTMLP
                     AIEHKLVKPTETFQCGRYQVGRRIISDTHQYGELTPREIITKSSNVGIAKIAERIGPT
                     RLYENFIALGFGQKTALNLAGESSGMFAKLQNWDGYTLHSIAFGQGISTTALQLATAY
                     SAIANGGKLMKPIIVETIKDDEGKIIEQFEPSVLRNVASTAATDTIKSYLQGVVDSGT
                     ARHIKMDYIQVGGKTGTAQKNITGTKGYSQGKYSSVFIGMFPIEKPQMVVVVFFDEPA
                     PGFHYGSTSSAPTFKKIVENILFMPDCQILPYNERLMQTSLTMPKLTGMHLFQAENTL
                     SHYGFQYKVEGPDSASIVIDQYPKAGVTVDPHYPITLKIGPNADKKAPVYETGTMPNL
                     VGLSLREALKQASVQGLAVNIRGSGMVRSQSVLPGSRILKGSKCYLEASL"
     gene            complement(6190..6546)
                     /locus_tag="PLG69_05780"
     CDS             complement(6190..6546)
                     /locus_tag="PLG69_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43055.1"
                     /translation="MRGRILILVSLFAIVFFLNFYNNNRIVNYTRQISALEKTYYAEK
                     NINTELLVELDDLRSGRHIASLVRVELSNFIPEKQAGKIIYVHEPSPKQEKKTYCIID
                     LLSKKAEAKNVQILLD"
     gene            complement(6543..7526)
                     /gene="rsmH"
                     /locus_tag="PLG69_05785"
     CDS             complement(6543..7526)
                     /gene="rsmH"
                     /locus_tag="PLG69_05785"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPI43056.1"
                     /translation="MTFHTPVMVKECLSYLNLRAGHIYVDATTGGGGHSLAMLKAQPE
                     IKLYCFDQDSEAIEEAGKILHNYNNVELIQANFKQLRTELAYRKIKGIDGILFDLGVS
                     SHQLECTERGFSFEKDAPLDMRMDKNLKNSAYNAVNELDTKTLTNIFREYGEEQNAYR
                     IAKAIEKSPKPIKTTSELAKIVESIAGKGTKASLKTKVRVFQALRIYVNKELESLSLA
                     LQDAINLLNPQGRIVVISYHSLEDRIVKQTFKKAEQKNEEQSVLPNSNKNHQQQLIIL
                     TKKPVTATEEEILVNPRSRSAKLRAGEKVQREKRETKTSRKPNRQNSQEEK"
     gene            complement(7523..7963)
                     /locus_tag="PLG69_05790"
     CDS             complement(7523..7963)
                     /locus_tag="PLG69_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MraZ"
                     /protein_id="HPI43057.1"
                     /translation="MSGEFLGIFENAVHKQRVIIPAAFKKKFSEAAEKKVVVTLGPNN
                     TIAIYPLDCWKATLERLKNGDEHCHKLRTQLIDFAMTEQELEGPGRVRIHEMLLNEVN
                     ITDSVVIKGEGHYISLWNPKVYNEVRASKLDQHRKQFTSEDYQL"
BASE COUNT         2429 a         1653 c         1464 g         2761 t
ORIGIN      
        1 tccgctgcga gccaaaccta taattccgta ttttttatct ttatctatca ttctatagca
       61 tcctttagcg tagcttgata gttgataagg caactgcagt aagcagggca gccacaatat
      121 aaaaacgcat cactattttg gattcgtgaa tacctttcag ctcgtaatga tgatgaaccg
      181 gagcacaaag aaaaactctg tgcccctcac cgtatttgcg ctttgtatat ttaaaccagg
      241 aacgttgaat aataacacta agtgcctcat atacaaagat aaagccaaga ataaaaaaga
      301 aaatttcctc tttcagcaaa atagaaatta cagcggcaat acctcctaag gttaaagaac
      361 cggtatctcc cataaaaact tctgccggat agctattaaa ccaaagaaaa cccattaaag
      421 ttcccattat agcactgatg aaaacagtca gttctcccgc actggaaata aattccagat
      481 tcaaataatt cgctgctatg tagtttcctt tcaaataact catcactccc aagcccaaaa
      541 aagaaaagat taaagttcct gctgccaatc catccaagcc atctgtcaaa tttacggcat
      601 tggaagttcc ggttataaaa agaacagcaa aaggaatgaa aacccaaccc aagggaacta
      661 ttaggttttt gaaaaaagga atttgcatag cagtaatatt ttttgagaag gaattgctgt
      721 agtagattgc caaggtaaga attaaaccaa cggaaatctg acctaaaagt ttataacggg
      781 gaattagtcc ttttttagct ttcacataat ttttcaaata atcatctaaa aaacccaaga
      841 tacctaacca aacggtagtt agaatgatca ttaaaattgc tgggttggtt aatacattcc
      901 ataaaagaga agaaatcagc aatgaaacca gaataattat acctcccatt gtcggagttc
      961 cctgtttcaa acgatgcctt tccggaaggt cttcatctat tgtctctaca gcagattttt
     1021 ttttcagcat tctaataaag gcaggaccta ccaatagaga tattattaga gaagtaatga
     1081 aagctcctat ggaacggaaa gttacataac ggaaaacatt aaaaacaatg ctatactttg
     1141 ccagagggta tagaagatga taaaacatta gtcctctcct ttcaagatag ggattacacg
     1201 ctccaactga attgcatgtg agccctttac taagataatg gcattttcgg gaagggaatt
     1261 caattttcgg tcagcaatca gttcttccac tgtgttgtaa tgaaaaatag ggctttcctt
     1321 ttccggatga taaatacggg aatagtttcc aacagtaaat aatttttcgt aacgcatttc
     1381 tgctaacatt gcggaaatca tttggtgata taaaatagag ctttctccca gttccagcat
     1441 atcacccaaa atagcataat gaggttgttc cggatttagt tcctgccaat attccagtgc
     1501 tttttgcatt gaataaggat tagcattgta gcaatccaga attaataatc ctgttcctct
     1561 tttttctacc tgacttctca attccagctg aataggttta tttaatgccg tttgcatctt
     1621 ttctttgtct attcccagtt ttaatcccaa aacaatggca aaagcagaat ttattgcata
     1681 gtgtggcgct ccataaggta aaaaccacaa gtcctcattc aagtaaaacg attgattatc
     1741 tttattacat ttatgccgag ttattctata atcgcaattc tcagcgtagc cgatgcttat
     1801 tccttcattg gtaagataat caaagcgaaa atcatcgcca ggatataaac gaaaagcaag
     1861 cggtctctta aaaagagtga ttttttctct aaaaacgcct tcttcatccc cgaaatattg
     1921 aagatgagaa ggtccgatat tcaaaataat accaatatca ggattagcta catcagccag
     1981 agcagcaatt tctccaaagt gattggttcc cagttcaaag attgcatact gctgttccgg
     2041 cttgattctg ataatagttt tacataagcc gattatatta ttttcatttg ccagtgtctt
     2101 taaaacggga gctgagcttt gcagaacttg agccattaat tctttacagg tagttttacc
     2161 tgtgcttcct gtgagggcaa ttctcttggc tgggaaaagc tgtaaatatt ttctgcagat
     2221 aagccccatt gtctcaaggc aattttccac ttgataatag ctattttcct ctgagaattg
     2281 attaagcgga taaggtattt ccccgattgc acaattctct ttaattttca ggacatctcc
     2341 cagatagctg tgtccatcgt atttttcacc tttaatagct ataaaaacac ttcccggctt
     2401 tatagaacgg gaatctatag aaagataata ataagaatat gtttcttggt atcccttagg
     2461 ttccggttcc caatttggca agagtaataa tttcaacagg gtttgatcta tcggcaaaat
     2521 caattcgtca tcggctttat cttttatttc tttttcggca agagctttca aggtttcttc
     2581 gcggtcatta aaatgatggc ggacaccttc aatttcctga taatcttcac ttccttttcc
     2641 acagataaga actatatcct cttcttgggc aagacggatt atggaagaaa ttgcctgttg
     2701 ccgatttctg ataatccacc aaggcaaaaa gatatctgaa gaggaaacaa tatctttaat
     2761 tatcgtttcc ggattttcac tacgaggatt atcgtcactg ataataacaa catcactatg
     2821 atttaaagct gatttcagca ttaaaggtct tttaccctta tctctatcgc ctccagcgcc
     2881 gattaaacag agaatccttt tatgaggcaa atcttccact gcttttaaca cattatctat
     2941 tgcttcgggg gtatgggcat aatcaataaa aatcccgata ttaaagttat tttccactcg
     3001 ttccaatctg ccttgaacgg gaggaatatc cttcagagaa ttgtgtattg cctcaggact
     3061 gaatcctaaa gcatataatg ttagagctgt taaacccagg ttttgaatat tgaaactgcc
     3121 aattaaacga ctggtaaccg gaatttcgca attttccgca atcagcttaa aactgctggt
     3181 gtttatatcc gtttttatat ccgagtattg aaattccgca tcattgcctc caagagaata
     3241 aacttttgcc tgcttttctt tcagcatatc ctttatctga actccaaaag catcagctgt
     3301 attgataaca gatatactgc catcttttgc tccttgttca aaaaagctca tcttggcttt
     3361 gccgtaattt tccagggttt gatgaaaatc aagatggtcg cgtcccagat tactgaatag
     3421 acagtagtca aatttaactc cgtaaatcct gtccagagaa agggaatgag aagagacctc
     3481 catcaccaca taatcaactt tcgcttctat catttgtcca aaaatctcat tcagttcaat
     3541 gatatcggga gttgttcttt cggaggggaa tgctttacct tcaatatagt aacccaaagt
     3601 tccaatccag ccgcaacgga agcccagatt tcttaatgct tcatagagca acaaggaagt
     3661 agtagttttg ccattcgttc cggttatacc tattaatgta taaggaattt tttccggcag
     3721 cattacggtt ctggcaataa cagcagctgc tttacgagta tctttcactt taatataggg
     3781 tatatctctt ttattgattt ccctgcagac aataatatca gcaccttgtt ccaaagcgga
     3841 attgatgaag ttatgtccat caaaattagc accttgaatg cagacaaata tatctccggg
     3901 cttaattttc cggttatctg tctgcacttt tccttgccag ttttccagct ttgctaaatt
     3961 tgctgtttcc acaaggagag aatgctgttg aagggcatat aggattctgt ttatcacaaa
     4021 gatgcctcca gatagcattt actgccttta agaattctac ttcctggaag cacggattgg
     4081 gaacgcacca ttcctgaacc gcgaatatta actgccagtc cttgaactga tgcctgtttt
     4141 aatgcttccc gtaaactaag tcctactaaa ttgggcatcg tgcctgtttc ataaacggga
     4201 gcttttttat cggcattggg tcctattttt aaggttatag gataatgggg gtctaccgta
     4261 actcctgctt taggatattg atctataaca atggaagcag aatcgggtcc ttccacttta
     4321 tattgaaaac cgtaatggct aagtgtattt tctgcttgaa agagatgcat tccggttaat
     4381 tttggcatag ttaacgaggt ctgcatcaag cgttcattat agggtaaaat ctgacaatcg
     4441 ggcataaaga ggatattttc cacaatcttt ttaaaggttg gagcgctgct tgtgctgcca
     4501 taatgaaaac cgggggcggg ttcatcaaaa aagaccacaa ctaccatctg gggtttttca
     4561 ataggaaaca tacctataaa tacggaacta tattttccct ggctgtaacc tttagtgccg
     4621 gttatatttt tttgagcggt tcctgtttta ccgccaacct gaatataatc catttttata
     4681 tgtctggcag ttccggaatc tacaactcct tgcagatagg atttaattgt atccgtagca
     4741 gcggtagaag caacattgcg taagacggag ggctcaaatt gttcaataat tttgccttcg
     4801 tcatctttta ttgtttccac aattatgggc ttcatcagtt ttccaccgtt agctatggca
     4861 ctataggcag tggcaagttg taatgcagta gtagaaattc cttgtccaaa agctatggaa
     4921 tgtaaagtat agccatccca attttgcagt ttggcaaaca ttccactgga ttcacctgcc
     4981 aaattcaaag ccgttttttg gccaaaaccc aaagcgatga aattttcata aagccgcgta
     5041 ggaccaattc tttcagctat tttagcaata cctacattgc tggatttggt tataatttcc
     5101 cggggagtta attctccata ttgatgagta tcggaaataa tccttcgtcc tacttgatat
     5161 ctaccgcact gaaatgtttc agttggttta acgagtttgt gttcaattgc tggtagcata
     5221 gttaagggct tcattgtaga gccgggttca aacataaaac taacaggaat attggatttt
     5281 accctcacga aattggggtc ttctgtttta tcttctccgg aaacacctgc catagctaaa
     5341 attttacctg tattggggtc cataacaata gcgccggcat ttttggctcc ataattttcc
     5401 atcccttcgt aaagggcatt ttccacaatc tcctgtatct tggcatcaat agttaaatat
     5461 aaattatatc cgttttccgg ctttttggaa tgcagattag gatagggaac aggttgatga
     5521 ttagcatcta aaacaacttc tttccaaccg tagtttccgg aaaggtattt atcgtaagtg
     5581 gattcaattc cgcagacccc agcaagtttg tagagggatt tactatttgt agcaggatca
     5641 tagccatcgg agacctcgcg cacagaacct aaaaccctgg cagctaaaat gtccttggaa
     5701 taaattcttt tcattgaggc aaagctgtga accaaaccgg gcatatcctt tgtctttagt
     5761 tccttgatta atttatccaa ttccacttcg cttattttat tgcttatttg aatagaactg
     5821 gttttatttc cataattcaa gcgctttatt acatcatctt tatttagagt ggtgtgagag
     5881 gatataacct cggcatactt ttcaaatgct ttttctaaag gtattttttc ttcttttgcc
     5941 caagatgcaa cagaactacg gtcaatatct atctgataat agattacgga gcttactaaa
     6001 agattacctt gggcatctaa tatagagccc cgacgcggaa tcagaatttc tttttggggt
     6061 gtataacgca ttcttcttgc gcctgccaga tgaaaagggt ctaatatctg aatgcagaat
     6121 aaatatgcaa tccagatcac agctaccaga gcaaaaataa caactaaaaa gtaataacgg
     6181 gatttcatct tagtccagca aaatttgcac attcttggct tctgcttttt tagacaacag
     6241 gtctatgatg caataggtct tcttttcctg tttgggagag ggctcatgca cataaataat
     6301 tttacctgct tgtttttcgg gaatgaaatt gctcaattcc actcttacta aagaagcaat
     6361 atgccttcca ctgcgtaaat catcaagctc taccagtaat tccgtattaa tgttcttttc
     6421 cgcataatag gtcttttcca gagcagatat ttgtctggta taattcacaa tgcggttgtt
     6481 gttataaaaa ttgagaaaaa atacaattgc gaatagggac acgagaatca ggattcttcc
     6541 tctcattttt cctcctgtga attttgtcgg tttggtttcc gactggtttt tgtttctctt
     6601 ttttcccttt gcactttttc tcccgcacgc aattttgcac tgcgtgaacg gggattaact
     6661 aaaatttctt cttccgtggc tgttacaggt tttttggtta aaataattaa ctgctgctga
     6721 tggtttttat tactattagg taaaaccgac tgctcttcat ttttctgttc tgctttttta
     6781 aatgtctgtt tcacaattct atcttccaat gaatgatagc tgataacaac tatcctgcct
     6841 tggggattta acagattgat ggcatcctgc aaagcaaggg aaagggattc caattcttta
     6901 tttacataaa ttcttaatgc ttgaaaaact ctaactttgg tttttaatga tgcttttgtt
     6961 ccttttcctg ctatgctttc tacaattttt gccagttcac ttgtggtctt tataggtttg
     7021 ggactttttt ctatggcttt ggctatacga taggcatttt gttcttctcc atattctcgg
     7081 aaaatattgg tcagtgtttt agtgtccaat tcattaaccg cattataggc actgttcttc
     7141 aggtttttat ccattcgcat atctaaagga gcatcttttt caaaactgaa accacgttcc
     7201 gtgcattcca gctggtgaga agaaacaccc aaatcaaaaa gaatgccatc aattcctttt
     7261 attttacgat aggcaagctc cgtgcgtaat tgtttaaaat tagcttgtat cagttctaca
     7321 ttattatagt tatgaagtat ttttcctgct tcttctattg cctcactatc ctggtcaaaa
     7381 caatatagct taatttcagg ttgggctttt agcatagcca aactatgtcc tccacctccg
     7441 gttgtggcat ctacataaat atgtcctgct ctcaaattta agtaggagag gcattcttta
     7501 accatcaccg gagtgtgaaa agtcataatt gataatcctc agaagtgaat tgtttacgat
     7561 gttgatcaag tttactggca cgcacttcat tataaacctt gggattccaa agtgaaatat
     7621 aatgaccttc acctttaata acaacactat ccgtaatatt aacttcattt aatagcattt
     7681 catgaatgcg cacccttcca ggtccttcca attcctgttc cgtcatcgca aaatcaatta
     7741 attgagtgcg aagtttatga caatgttcat caccgttttt taagcgttcc agtgttgctt
     7801 tccagcaatc caaaggataa atggcaatcg tattattggg acctaaagta acaacaacct
     7861 ttttttccgc tgcttccgaa aatttcttct tgaaagcagc tggaattatt accctttgtt
     7921 tatgcacagc attttcaaag ataccaagaa attcaccaga catagctgac atcctttttt
     7981 tccggtttcc taaaaaaacc gttctttgct ttactgtccg tgcttttttg tctcatttcc
     8041 gtgaaccacc ttgagatttt ttgagttttt ctgagttcct gtggttctta tatgacactt
     8101 cgtttttctt gtcaagtgca aaatgcattt tttttcaaat ttctaaatta ctgttctcct
     8161 tatttcagtt gaaagacgaa tgtttattat tttgttatac ttggagttag aattgttata
     8221 tgtagtattt gctattttag agtaatggat gtaactatct gaagggcaat aaataataaa
     8281 ttgagaaaaa attttttgta tttgttt
//
LOCUS       DAOPFL010000075         8218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_11001_length_8218_cov_107.681735, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000075 DAOPFL010000000
VERSION     DAOPFL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11001_length_8218_cov_107.681735"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3010)
                     /locus_tag="PLG69_05795"
     CDS             complement(<1..3010)
                     /locus_tag="PLG69_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43058.1"
                     /translation="MSDLTKYIQKYRQRINLRFALQAIIKALISFLLALHIYFLLWLN
                     MPSQSMALFYANIGIRTCLILVIIYFLLSGYRNFWHNLAVARFLDKQIDFKDDLFQNT
                     LELAEKEKDSPIVENLGTMAKKRIEENRYKIPALYPSWVVFAILFLILGLGTIWAYSY
                     EDFRLAFKQFYTNQGQEVIYKNFIELSPGNLTIGRNEAVEIKILNPDPRLKHRLFYRI
                     DKQWRELGLADNRYIFQRLDNSIEYYAENEVCKSPVFKIQVLDEPIVKKWFVQYNPPA
                     YSGLPTWTDTLSYGNIEGLKHSRVKLSLTTNIPVESAVMVFDDASRLPLQAIDKNNYI
                     TQLAIEKPCSWYLELTDALGRKNKPEEKHIRILEDNPPEINITFPGQDVNLNQNMLLP
                     LIIEADDDYGLKNCTLSIQIMTSEPQVVNVQSVIPGKMFVTDFLLDLKDANLFPGDVV
                     TYWATIYDNSPEQQKAESTRFKARFPSIEEIYREIEAREQEKKTELETALQKSKDLQK
                     EFENKRRELLKQDNPKWEDKKQLEKILQEQENLSQQVQNIADNYQELINKMQANETLS
                     PETLQKMQKIQELMQEINNEDLQKAMEKFSNTLQNIKPEDLKKAMENFKFSMEDFSKK
                     IEQTLALLESIKKEQAIQKALQISEEMEKMQKTLSERTMDEKQNYKDLASDQKQISDT
                     YDKLQQELDKIDKMLESPRDNQVINQLKDLKQDMQNSNLKQDLQNSQNALSQNQRSQS
                     LSAQNQALEKIRRFSLKLNQMKESMGGGNQQRTMQAIQTAIRELLIFSKQHEATASRY
                     RNDPYVIVQDLIAQSEGIQISLNKMFRETQVLMIIPPKFFIDVNETNSAYRNIFSYIN
                     ESQFYQIPNALNSVQKGINLMVYDLMQALQNASSGTGSGGGGMQSLMQMLEQMSQEQM
                     AMNILTEQLFLQMQAQGGKMDSAMQQQIQRLAEDQERLAENLKRALQNNPEAQKQGNV
                     LKQITEEMDAITRQLKNNQLNPDILERQERI"
     gene            4084..6195
                     /gene="asnB"
                     /locus_tag="PLG69_05800"
     CDS             4084..6195
                     /gene="asnB"
                     /locus_tag="PLG69_05800"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPI43059.1"
                     /translation="MCGIAGIYCFSKETVILDKKQDNFSKGKDNPVKNGVSFKQNVAN
                     ALLLKEMTDAIKHRGPDDEGYLIKDKNGIRSFSGVDSPAEIQSVYPLFNVEIDSTFPA
                     KGYSSVNSFKLGNFILGMGFRRLSILELKPIGHQPMYDKELGIAICYNGEIYNYLELK
                     AELKAKGYKFFSNSDTEVIIKAYHFWGEDCVLHFNGMWSFSLWDERKDLLFCSRDRYG
                     IKPFYYAVQDGVLYWGSEVKQLLLTPIDKKLNQAMIWRSMKINSFLVYDDETYWQNVH
                     SLKPGHNLLAVNGKIIINQYYDLDIANFESSQLSFHKAVEQYRNIFLDSISLQLRSDV
                     EIGATLSGGMDSSAIVCSAVQKKGEPLKTFSSYYAFTPELDERKWMEKIVQKTSCTSF
                     LVSPTAEDAICWWEKLTYMNDLPLSAAFVSINAVMQEAHKQGIKVLLSGQGSDEISAG
                     YRHSLYRYFADLIRGMQISRLGKELPIYLKKDNKAFSKLGKIMLSTFLPESSLYNLEF
                     CYYRFEPFNRAFTKEAEKNCGEQILKKIVDIKASRLSNFLYNMMHNTSLQTLLHFEDR
                     LTMANSVESRVPFLDYRLVDFVFSLPSQYKVQPPYTKVIHRFAMKDLIPEEIYYRQDK
                     GIFSSPFYQVWMKQELKPFISDIFASSAFRQRGIWNLPKINHYWHKYLAGDNKQVEML
                     FNVIALELWFRQYVDKPSGEY"
     gene            6195..7079
                     /locus_tag="PLG69_05805"
     CDS             6195..7079
                     /locus_tag="PLG69_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43060.1"
                     /translation="MKNILYLVAFSLVLISLSSCKLFKSPTVTKIQDVKVISITPDKS
                     TIKISFMVSNPNCYKLKLDQLNVELLNKDRYKVGTATLQKVVQIPKKSTMCLDFNVEL
                     ETRPVVKMVSSIDQKVQFFITGKGHGKVLGIGKKFKFDEPYELDLKEQLKSIIPKFST
                     NGQSLFKITKTTVEKIGIGETELQVHFIILNPYGLTFNFKGFPAEIYISDKFAGTGNL
                     KNQLKFNENIYSQDGVMVFKLNNLKTILGAVKGAVKGEIRYSVKGRVLIDALGMEINQ
                     PYSYQDALPVNILEMLLN"
BASE COUNT         2475 a         1450 c         1526 g         2767 t
ORIGIN      
        1 taattctttc ctgtctttcc aggatatcgg gattgagctg attatttttc agttgtctgg
       61 ttatggcatc catttcttct gtaatttgtt tcaggacatt tccctgtttt tgtgcttccg
      121 gattattttg cagggctctt ttcaggtttt ctgcgaggcg ttcctgatct tctgccaggc
      181 gttgtatttg ttgttgcata gcactatcca tttttccgcc ttgagcttgc atttgtaaaa
      241 acaattgttc ggtgaggata ttcattgcca tttgttcctg gctcatttgt tccagcattt
      301 gcattaggga ttgcatacct ccacctccgc ttcctgttcc ggaagaagca ttttgcaatg
      361 cctgcattag gtcatatacc atcaggttta ttcccttttg aacgctgttt aaggcattgg
      421 gaatttggta aaactgcgat tcattgatgt agctgaaaat atttctgtag gcgctattgg
      481 tttcattcac atcaataaag aatttagggg gaattatcat cagcacctgt gtttcccgga
      541 acatcttatt taaggaaatt tgaattcctt cggattgagc aataaggtct tgcacaatta
      601 catagggatc atttctataa cggctggcag ttgcctcatg ctgtttggaa aaaatcagaa
      661 gttctcttat tgcagtttga attgcctgca ttgttctttg ttgattaccc ccacccattg
      721 attccttcat ctgattcagt tttaaggaaa aacggcgtat tttttccagt gcctgatttt
      781 gagcagaaag ggattgactg cgttgatttt ggcttaaagc attctgactg ttttgcaggt
      841 cttgtttcag attgctgttt tgcatatctt gtttcaggtc ttttaattga tttataacct
      901 gattatcgcg cggggattcc agcattttat caattttatc caattcctgt tgcagtttat
      961 cgtaggtatc actaatttgt ttttggtcgg aggcaaggtc tttatagttt tgtttttcat
     1021 ccattgtgcg ttcgctaagt gttttctgca ttttttccat ttcttcggaa atctgcaatg
     1081 ctttttggat tgcctgttct tttttaatgc tttccagcag ggcaagtgtc tgttcaatct
     1141 ttttgctaaa atcctccatt gagaatttga aattttccat tgctttttta aggtcttccg
     1201 gtttaatgtt ttgcagggta ttagagaatt tttccattgc tttttgcaaa tcctcattat
     1261 taatttcctg catcagttcc tggatttttt gcattttttg caaagtttcg ggagagaggg
     1321 tttcatttgc ctgcatttta ttaatcagtt cctgatagtt atcggcaata ttttgcacct
     1381 gctgggaaag attttcctgt tcctgcaata ttttttccag ctgtttttta tcttcccact
     1441 tgggattatc ctgtttaagc agttcccggc gtttattttc aaattctttt tggaggtctt
     1501 tggatttttg taaggcggtt tccagttccg ttttcttttc ttgttcgcgg gcttcaattt
     1561 ctctataaat ctcttcaatg gaagggaaac gagctttaaa tcttgtgctt tccgcttttt
     1621 gttgttcggg agaattatcg taaatagttg cccaataagt aactacatca ccagggaata
     1681 aattggcatc tttcaaatcc agcagaaagt ccgttacaaa catttttccg ggaataacac
     1741 tttgcacatt aacaacttgc ggttccgaag tcataatttg gatgctcaaa gtgcaatttt
     1801 tcaatccgta atcgtcatct gcttcaatga ttaaaggaag tagcatattt tgattcaggt
     1861 taacatcctg acctggaaaa gtaatattga tttcaggagg attatcttcc agaatacgaa
     1921 tatgtttttc ttcgggttta tttttgcgac ctaaagcatc agtaagttcc aaataccaac
     1981 tgcagggctt ttcaatggca agttgggtga tataattatt tttatctatt gcctgtaaag
     2041 gaagacgact ggcatcgtca aaaaccatta cagctgattc tacaggaata ttagtggtga
     2101 gggaaagttt tactctgctg tgctttaatc cctctatatt gccgtaactt aaagtatccg
     2161 tccaggttgg caaaccggaa taagcaggcg gattatattg cacaaaccac ttttttacaa
     2221 tcggttcgtc caaaacctga attttaaaca ccggggattt gcagacctca ttttccgcat
     2281 agtattcaat actattatca agtcgctgaa aaatatagcg gttatctgcc aaacccaatt
     2341 cccgccattg tttatctatc ctgtaaaaaa gacgatgctt caaacgcgga tcgggattta
     2401 ggatctttat ttctaccgct tcatttctgc caatagtgag gttgccaggg cttaactcta
     2461 taaaattctt atagataact tcctgacctt gattggtgta aaattgcttg aatgccaaac
     2521 gaaaatcctc ataagaatat gcccaaatag ttcctaaacc caagattaga aagaggatag
     2581 cgaagacaac ccaggaagga tatagagcag gaattttgta acgattttcc tctatccgct
     2641 tttttgccat tgtgcccaaa ttttccacaa tcggactatc tttttctttt tcagcaagtt
     2701 ccaaagtatt ctgaaaaaga tcgtctttaa aatcaatctg tttatccaaa aaacgagcga
     2761 ctgccaaatt atgccaaaag ttacgataac cacttaaaag aaagtaaatt atcaccagga
     2821 taagacaagt ccgtatgcca atattcgcat aaaaaagtgc cattgattgc gaaggcatat
     2881 tcaaccataa caaaaaataa atgtgcaaag ccaacaaaaa gctgattaga gctttgataa
     2941 ttgcctgcaa agcaaagcga agattaatcc tttgcctgta tttttggata tattttgtca
     3001 ggtcgctcat ttttttgtct tctcctcttc tttgaaaaat ctttgtccta taaagcactt
     3061 attgtgtcaa gaagttttgc aaatttagct ggaggacgct gttcttccgc attctttttt
     3121 ttccttttcc cgctcaaaaa aagcccatca gggcgacata atgatagcga tggatgcgta
     3181 agcccctcgt aaataattaa aaacattaca tcctaataac gatttattat tctatattct
     3241 gcataaaaat tgaccataaa ttccggcatt tatctcacta ataatccacc accaaaattt
     3301 ctatcatatc tccctaacat cttatatcat aagtgattat ctatatattt tgccttttct
     3361 attcctaatc gcttaattac ctgaaacata atctcttgca agaagacccc gtaacacatc
     3421 cgtaacactt ccctaacact gcagtagttc cgttacttgg tcgttacttg aatgttacaa
     3481 gattattact tcagcaagtg aacgagtatt ttattccaaa taatgcaatt gcgtgaaaat
     3541 aggttggcaa cttccaattt gccaaagagt tcgtagaatc ctacaaaaac atttatactt
     3601 ttttttgctg aatcagaagt tggtaaccca aaaggtaaat ttgtcttatc ttctcagagt
     3661 taaaaccctt ttaaaatatc tgtctttcct atgtaattat ctcactttct tagcgatatt
     3721 ttttcgtcag ttttacacac ttatttagct aactgccatt tctacggaat ttttgctaca
     3781 atatatgatt aatgaatatc aaaataatat taaccattat ttctacggca taatttgagt
     3841 ttatacatat ttatttccat aataggatat taagagagaa aaatatataa ataaggttat
     3901 cataatttac aggattgatg gataaaaagg aaaaaatcat ataatctaag aaatggaaca
     3961 ggtaaaccat cagagcaatc ataactttga taatttaata aatttgttcc ttatatcaaa
     4021 tgataaaacg cttgactgga aatgtaatta tgaaaaaagg aattaacaga aaaaggagaa
     4081 tgaatgtgtg gaatagcagg tatctactgt tttagtaaag aaacagttat tttagataaa
     4141 aaacaggata atttcagcaa aggaaaggat aacccggtta aaaatggggt cagttttaag
     4201 caaaatgttg ctaatgcact gctattaaaa gaaatgacgg atgctatcaa gcatcggggt
     4261 cctgatgatg agggctattt aattaaggat aaaaacggaa ttaggtcttt ttctggagta
     4321 gattcaccag cagaaataca atcagtatat cctttattca atgttgaaat agacagcact
     4381 tttcctgcta agggatattc ttcggttaat tcttttaagt tgggtaattt tattttagga
     4441 atgggtttca ggcgcttgag cattttggaa ttgaaaccta ttggtcatca accgatgtat
     4501 gataaagaac ttggcatagc aatatgttat aatggtgaaa tatataatta tctggaacta
     4561 aaagcagagc ttaaggcaaa gggctataaa tttttcagca atagcgatac agaagtaata
     4621 attaaggcgt atcatttttg gggtgaggat tgtgtgcttc attttaacgg aatgtggtca
     4681 ttttctttat gggatgaaag aaaggatttg cttttttgca gtcgtgatcg ctatggcatt
     4741 aaaccctttt attatgcggt tcaggatggt gtattatatt ggggcagtga agttaaacag
     4801 cttttgctta cccctataga taagaaatta aaccaggcaa tgatttggcg cagtatgaag
     4861 ataaattcat ttctggttta tgatgatgag acctattggc aaaatgttca ctctttaaaa
     4921 ccggggcata atttgcttgc tgtaaacggt aagattataa tcaaccaata ttatgacttg
     4981 gatatagcca attttgaaag ttcccagctt tcttttcata aagcagttga acaatataga
     5041 aatatctttc tggattccat ttccttacaa cttcgcagtg atgtagaaat tggagctacc
     5101 ttgtccggag ggatggattc ttcggcaatt gtttgttctg cagtgcaaaa aaaaggtgaa
     5161 cctctaaaaa cattttcttc ctattatgct tttacaccgg aactggatga acgcaagtgg
     5221 atggaaaaaa tcgtgcaaaa gacctcctgc acttcgtttt tggttagtcc tacagcagaa
     5281 gatgccattt gctggtggga aaaattgact tatatgaatg accttccctt atctgcagct
     5341 tttgtatcta tcaatgcagt gatgcaagaa gcacataaac aaggaattaa ggttttgctt
     5401 tcgggacagg gttccgatga aatatctgct ggttacagac attctttata ccgttatttt
     5461 gccgatttga ttaggggtat gcaaattagc agattaggga aagaactgcc tatctatttg
     5521 aaaaaagata ataaggcatt ttccaaactg ggaaaaataa tgctttctac ttttctgccg
     5581 gagtcatcac tctacaatct ggagttttgt tattaccgct ttgagccctt taatcgtgct
     5641 tttacaaaag aagccgaaaa aaactgcggg gaacaaattc tgaaaaagat tgtggatatt
     5701 aaggcatcac ggctttccaa tttcctgtat aatatgatgc ataacacttc cctgcaaaca
     5761 ttgttgcatt ttgaagaccg gttgactatg gcaaattctg tggaaagtag agttcctttt
     5821 ctggattatc gtttggtgga ttttgttttt tctctgccat cacaatataa ggttcagcca
     5881 ccttatacca aagtaattca ccgttttgca atgaaagatt tgattccgga agaaatatat
     5941 taccggcagg ataaaggcat tttttcttcg cccttttatc aggtctggat gaaacaggaa
     6001 ttaaaacctt ttatttccga tatttttgcc agcagtgcat ttcgccaaag aggaatttgg
     6061 aatttgccca agattaatca ttattggcat aaatatttgg caggagacaa caaacaggtg
     6121 gaaatgctat ttaatgttat cgctttggaa ctttggttca gacagtatgt agataaacct
     6181 tcaggagaat attgatgaaa aacatattat atttagtagc ttttagctta gtgcttattt
     6241 ctctcagttc ctgcaaactc ttcaagagcc ctacagtgac aaaaattcag gatgtgaaag
     6301 tgatttccat cactcccgat aaaagcacta ttaaaatatc gtttatggta agtaatccta
     6361 attgctataa attgaaactg gatcaattga atgtggaact gctaaataaa gaccgctaca
     6421 aagttggaac tgctactttg caaaaggtgg tgcaaatacc taaaaaatca actatgtgtt
     6481 tggattttaa tgtggaactg gaaacgcgtc cagttgtgaa aatggttagt tccatagatc
     6541 aaaaagtgca atttttcata accggtaaag gacatggcaa agttttgggg attgggaaaa
     6601 agtttaaatt tgatgaaccc tacgagcttg atttgaagga acaactgaaa agcattattc
     6661 ctaaattcag caccaatgga caaagtttat tcaaaataac gaagaccact gttgaaaaga
     6721 tcggcattgg cgaaacggaa ttacaggttc attttattat tttgaatcct tacggtttaa
     6781 ccttcaattt caagggcttt cccgcggaaa tttatatcag cgataaattt gccggaacgg
     6841 gaaacctgaa aaatcagctt aaattcaacg aaaatatcta ctctcaggat ggagtaatgg
     6901 tttttaaact gaataatctg aaaaccattc tgggagctgt aaaaggcgca gttaaaggag
     6961 aaataagata ttccgtgaaa ggaagagtcc ttattgatgc tttgggaatg gaaattaacc
     7021 aaccctattc ctaccaggat gctttaccgg taaatatttt agagatgctg ctgaattaaa
     7081 cttaaataat acaattgagg aaaaatgggt tggcaacttc ctctttgcca aagagttcgc
     7141 agaaacctga aaaatatatg tcctctattt atggtaactc agaattcagt taaccaaata
     7201 ataaatttga attttttttc caggttaaaa cctctctata ctatctgtcg ttcctacgga
     7261 acttttttcc ccaataaata tagttttgta ggttcttttc atatcaccgg gttgaaaccc
     7321 gttgttagta tctgtcgttc ctacggaact ttttctcgga aaaggagata atgactctcc
     7381 tgctttctta tcaccgtgtt gaaacccgtt gttagtatct gtcgttccta cggaactctt
     7441 tctcggaaaa ggagataatg actctcctgc tttcttatca ccgggttgaa acccgttgtt
     7501 agtatctgtc gttcctacgg aacttttctc ggaaaaggag ataatgactc tcctgctttc
     7561 ttatcaccgg gttaaaaccc gctgttaaaa tcaaaaaaat cccgttaggg atggcagata
     7621 ctaacaacgg gttttgaacc cggtgaaaat gagaaaaacc ttaccactat aataaaaagt
     7681 tccgtaggaa cgacagatac tattagcggg tttcaacccg cacaaagcaa caagcagaaa
     7741 aagtaaaaga tccagcagtt aaagatacaa taaaatagat aggacttatg tagagaaaga
     7801 agtcattagc cagataaaat aaatatttat cagaaaaaat aaaaattctg tggggctacg
     7861 gaactattca gacaagtaat ggtggttgta tttggattat tcttttcacc gggttaaaac
     7921 ccgttgttag tatctgtcgt tcctaaggaa ctctttctcg gaaaaggaga taatgactct
     7981 cctgctttct tatcaccggg ttgaaacccg ttgttaatat ctgtcgttcc tacggaattt
     8041 ttactacaat atatgattgc tgattaggga aataacatta atcattattt ctactgcatt
     8101 atttgggtta aacttaaaca atgcaactgg ttcaattatg aaccatattt cgtaattgga
     8161 attcggaatt ccggtttggc aaaattcaat agtgtgcagg acacgatttt tcttgaca
//
LOCUS       DAOPFL010000076         7640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_12247_length_7640_cov_97.117073, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000076 DAOPFL010000000
VERSION     DAOPFL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12247_length_7640_cov_97.117073"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLG69_05810"
     CDS             <1..669
                     /locus_tag="PLG69_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43061.1"
                     /translation="KMEYTFEAIKFFQTRIEAYLNIWENATLEDMKSDQREKQHSPLN
                     VRKALFRQAIGGHLTEGQEYRLISQDLKWYLHPEFKTFNRELRTLIEEKLEEKNLLYY
                     WEPREYEEIAPEKLQGPFEREFNCWKYVNLDYDDGDLGKDEAEISLKARWVLHCAYKA
                     NKLSEAEITQLIKLDLDALLCENAVYFNIFELKLITDFLLEQGVWDHLPDPLFVNRLS
                     SQPE"
     gene            complement(1476..1943)
                     /locus_tag="PLG69_05815"
     CDS             complement(1476..1943)
                     /locus_tag="PLG69_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPI43062.1"
                     /translation="MKWYLIVGLTVMVMIATVLVRIPVPGGGYFNLGDVVIIFSGLFG
                     GWKAGLIAGGIGSALADLIGFPVFAPITLIVKGLEGLVAGLAKPAVKWQIYFFPAIAV
                     IIMIAGYFLGCWLFPNLGLAVAIADLPVNIIQAVAGYIGGIALHLAYTKISST"
     gene            complement(1978..2589)
                     /locus_tag="PLG69_05820"
     CDS             complement(1978..2589)
                     /locus_tag="PLG69_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPI43063.1"
                     /translation="MKPQYRLLLVSLALIVFFVFFCLIYLENIPVQLVVLGVVLLLSA
                     WTFKLKGLLKKLYHFLPFILLLFGVYFIFAFFQIGQNKDYWIHYGITRTTLLISSLMF
                     IQVLITWLKIDTFLDFPLGIEKLKYIILGKMLYKIAFSSYSELCLFVDSIPAEQAGTI
                     TLKKKFRKRLIVLLALITYVINEATLKGEMIDERIWHCHQVPK"
     gene            complement(2573..3097)
                     /locus_tag="PLG69_05825"
     CDS             complement(2573..3097)
                     /locus_tag="PLG69_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43064.1"
                     /translation="MIKIAPGIVSCWQDFSLLIEQELFFLPENIYYLQGENGSGKSSF
                     IKHSLLPVLETQRNLFYFLYFQQLFHLQGYAIKSHSAFYQPELKLKSEWDCIQYLLHN
                     LSEIYAIESKPVYCIVDENLRLAEIYHYLKESGIPFCLIFCEHSSFSITEEVNIINFQ
                     LIAPNQSRVYETTI"
     gene            complement(3094..3438)
                     /locus_tag="PLG69_05830"
     CDS             complement(3094..3438)
                     /locus_tag="PLG69_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43065.1"
                     /translation="MIHKDYLWADANIPLISCDRLRDELLIYNLDESATANLIDQFEK
                     LTGKKIDKFYKIEELSGGQKVILMALLAIYSPAPKIRFVNLLNALDPKRRDAIQSLIR
                     NSGKDIVLDESG"
     gene            complement(3459..3551)
                     /locus_tag="PLG69_05835"
     tRNA            complement(3459..3551)
                     /locus_tag="PLG69_05835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3515..3517),aa:Ser,seq:cga)
     gene            complement(3560..4027)
                     /locus_tag="PLG69_05840"
     CDS             complement(3560..4027)
                     /locus_tag="PLG69_05840"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPI43066.1"
                     /translation="MICFSSQNHYLFMQEAIAEAKKAFTEDEIPVGALLVKNNTIILK
                     EHNRSRQLANPLAHCEKLLIDKILTSEPGFLYDYTLYVTLEPCLMCAGMIILSKIGTV
                     VYGAKDPKTGVVGSLYNVLNDKSFNHHPVVIGGILEQECAFLLEEFFRKKRTL"
     gene            complement(4024..5721)
                     /locus_tag="PLG69_05845"
     CDS             complement(4024..5721)
                     /locus_tag="PLG69_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPI43067.1"
                     /translation="MNKLFTFIVITLLGLFFLVSCATNSSSVAGSVNGTEISLEDFFN
                     SYRGHYAIFGYQTGRTPTKEEKEKLHNETWRDITKAIILQDYYKKYNISASVAEVLDT
                     LSNSIPEHIVKSPRFQVNGKFDKKLYLQSLMTDQPENLAALRKHYQENVIPILKLQKV
                     LIEEEMLSSKIRKQVEQIIASNADLELYIFAPSQLEISLSDAEISAWYQTNLEQFRLW
                     QFYRLGYCSIPVIPDSTELSDAKNTAEFIRNELARGIEIKDIIANLENKNSLVSFIDN
                     GYQKTEELPWEVQNALSGLENGRCSEPLPYEKGWIMYQKIQSTKTLTLYNTIFVQALP
                     KTASLLAPETQAQQIMKLALNIGLQQAADEFGISYTLTPAMSPDSLIVPANDIKGKLM
                     KYLEIANSGDIIEPLYSAELSAWLIFEVVEKQSKEYLPLEEVKPQIVQILSKERRKDQ
                     NKQIVERWLINQNIPANYQWEKLEQVTIDSLWQGKPLTRIYYQAVKAYLEKANPPQIT
                     EGEVIIVPKVTAFRPQKTKISPERIKTIYTQNLPEDWFNSWMEEQVKKAKVVINTKP"
     gene            complement(5718..>7640)
                     /gene="mutS"
                     /locus_tag="PLG69_05850"
     CDS             complement(5718..>7640)
                     /gene="mutS"
                     /locus_tag="PLG69_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPI43068.1"
                     /translation="AAGAALAYVQGLYNSPLNHISSLRYYSLSQYMQLDEISRRNLEL
                     VRSIRYGTKYGSLLSVIDQTMTPMGSRLLQQWLLHPLLDIKEITFRQDIIQSFIDKSS
                     HLKELRLILKEIGDITRLVTRLGSLRINPRELIALKSYLYSARNLQTKLSTFEHPQFA
                     VWQQNMGSFEDIIALIEKAINDNPPVSITEGGIFAKGYNPELDELMDIIYDGKSWIAR
                     LEEDERRKTGINNLKVGYNRVFGYYIEVSSANKNKVPDYYIPKQTLTNSERFISPRLK
                     EFEAKVLSSEEKIKNLEYELFKELRQNLAESLPRFQQLSEVIAELDVLSSLAFLAWQN
                     QYSRPVFTESRELHITEGRHPVIEKLMESDKFIPNDTHLDYPETSIAIITGPNMAGKS
                     TYLRQVGLLVILAQMGSFVPASKMTLPVFDRVFTRVGASDNLAQGQSTFLVEMIETAN
                     ILHSATSNSLILLDEIGRGTSTFDGLSLAWAIIEYIQKYKHSLTLFATHYHELTELEN
                     LYPDIKNYNVAVKQWNEEMIFIRKIERGGADQSYGIQVARLAGIPEKVIRRAKEILKN
                     LEEHEISPQGLTATIRKKLVRDVPQIDIFEILADKASENDPIINEIKEIDLNKLSPIE
                     AFQYLQKIQNQLLGEK"
BASE COUNT         2434 a         1463 c         1379 g         2364 t
ORIGIN      
        1 aaaatggaat acacctttga agcgattaaa ttttttcaga cgcggattga ggcatatctt
       61 aatatttggg aaaatgctac tttggaagat atgaaaagtg atcaaaggga aaaacagcat
      121 tcacctttaa atgtgaggaa agcattattt cgtcaagcta taggaggtca tttaactgaa
      181 gggcaggaat atcgcttaat aagtcaagac ctaaagtggt atttgcatcc ggagtttaaa
      241 acctttaacc gggaattgag gactctgata gaagaaaaat tagaggaaaa gaatctgctg
      301 tattactggg agccgagaga atatgaagaa attgccccgg aaaaacttca aggtcccttt
      361 gagcgtgaat tcaattgctg gaaatatgta aatctggatt atgatgatgg tgatttgggc
      421 aaagatgaag cggaaatatc cctaaaagca agatgggttt tgcattgcgc ctataaagct
      481 aataaacttt ccgaagcaga aattacccaa ttaatcaaat tagatttgga tgcactgttg
      541 tgtgaaaatg ctgtttattt caatattttt gaactgaagt tgataacgga ttttttactg
      601 gaacagggag tttgggatca tttacctgat ccgctttttg tgaacaggtt atcttcccaa
      661 ccggaataat ggttatctat ttttttttgt aagttgtgca tttttgatgt ggtagttgtt
      721 attttgcaaa ataggatagg gtcttccgga attcttctct gataatttca ataatgcggt
      781 tggggagaaa aaaaggagtg gataattccg aattgaaaag aaaacagaaa atttttgaat
      841 aaatttatgc ctggggttta tcccgaatcg taattcctca ccctaaatac caaatacacc
      901 cactattgtg ttcggatgca ttatactgac attaccgact gcaactttta aagacaaagt
      961 aacactcaag taacaactcc gtaacggaac tacggaagtg ttacggaagt gttacggagt
     1021 cgttacttaa ttgactatat aacaagcaac tatctgctgt ctaattgtca ttcctacaga
     1081 ggcagggatc tacacaggac aaaataagaa tctatagttt ccttttgccg ggttgaaacc
     1141 cgtcgttagt atctgtcgtt cctacggaac tcattcttaa aaagagagat aagatgtatc
     1201 attttgcact tatcaccggg ttaaaacccg ttgttagtac ctgtcgttcc tacggaactt
     1261 tttttaggaa gatgaagaaa agaatattct ggactctttt tcaccgggtt aaaacccgtc
     1321 gttagtatct gtcgttccta cagaactctt tctcaaaaaa agagataaga tgtttcattt
     1381 tgcacttata gccgggttaa aacctatcgt tagtatcttt tgttcctaca gaactcaaaa
     1441 aaaagaaata aacccgaata atgcactcgg aaatgctaag tactggaaat tttggtgtaa
     1501 gcaagatgta aggcaatacc tccaatataa cctgcaactg cctgaataat attgaccggc
     1561 aaatcggcta tagctactgc caatcccaga ttaggaaaaa gccagcaacc caaaaaataa
     1621 ccggcaatca taattataac tgctattgcc gggaaaaaat aaatctgcca ttttacagcg
     1681 ggttttgcca atccagcaac caatccttcc agccctttaa caataagcgt tataggggca
     1741 aaaacaggaa aaccgattaa atcggcaaga gccgaaccaa ttcctcctgc aattaaacct
     1801 gctttccagc caccaaaaag tccactaaag ataataacta catctcctaa attaaaatag
     1861 ccacctcctg gaacaggaat tcttaccaga accgtagcga tcataaccat aacggtaagt
     1921 ccaactataa gataccattt cattttatct cctttttatt catgtattta ggcaaaatta
     1981 ctttggaact tggtgacaat gccagatacg ttcatcaatc atttctcctt tcagtgttgc
     2041 ctcattaatt acataagtaa taagtgctaa aagaacaatg aggcgtttac gaaacttttt
     2101 tttcagagta attgtacccg cttgctcagc aggaatggaa tctacaaaaa ggcataactc
     2161 ggaataagaa gaaaaggcaa ttttataaag cattttaccc aaaataatat acttcaattt
     2221 ctctattccc aaaggaaaat ccagaaaggt gtctattttc agccaagtga ttaatacctg
     2281 gataaacatt aaactgctga taagtaatgt agttcgcgta ataccgtaat gaatccagta
     2341 atccttgttt tgcccaatct gaaaaaaggc aaaaataaaa taaaccccga aaagcagtaa
     2401 aataaaaggt aagaagtgat aaagtttctt taaaagtccc ttaagcttaa aagtccaagc
     2461 actaagcaat aatacaactc ccaagacaac taattgcaca gggatattct ccagataaat
     2521 aaggcagaag aaaacaaaaa acacaattag agctaaagag accaacagta atctatattg
     2581 tggtttcata aacgcgactc tgatttggtg caatcaactg aaaattgatg atgttaactt
     2641 cttcggtaat agaaaaagaa gaatgttcac aaaaaatgag acaaaaaggg ataccgcttt
     2701 cttttaaata gtgataaatt tctgctaaac gcaggttttc atctacaatg caataaaccg
     2761 gtttagattc aatagcatat atttcggaga gattatgcag cagatattga atgcaatccc
     2821 attcgctttt cagtttcaat tccggctgat aaaatgcgga atgggactta atagcatagc
     2881 cctgcaaatg aaaaagttgt tggaaataga gaaaatagaa caaattcctt tgtgtttcca
     2941 aaacgggaag caaggaatgt tttataaaag aacttttacc actgccgttt tcaccttgca
     3001 gataatagat attttcaggc agaaaaaaaa gctcctgctc aatcagcaaa ctaaaatcct
     3061 gccagcaaga aactatcccc ggtgcaattt taatcatccg ctttcgtcca gaacaatatc
     3121 ctttccgcta ttccgaatca agctttgaat tgcatctcgg cgttttggat caagcgcatt
     3181 cagcaaattc acaaaacgga ttttaggtgc aggagagtat attgccaaaa gtgccatcaa
     3241 aataactttc tgccctccgg aaagttcctc tattttgtaa aatttatcta ttttcttacc
     3301 agtcaatttt tcaaactggt caatcagatt agctgtggct gattcatcca aattgtaaat
     3361 aagaagttca tctcttaagc ggtcacagga aataagcgga atattggcat cagcccataa
     3421 ataatcctta tgtatcattt ttcatttcct ttatcaactg gcggagagtc agggattcga
     3481 accctgggta cctgtaaaaa aggtacaacg gttttcgaga ccgtcccgtt caaccactcc
     3541 ggcaactctc ctctttattt tataatgttc tttttttgcg gaaaaattcc tccagcagga
     3601 aggcacattc ttgttccaga attccgccaa taacgacagg atgatgattg aaacttttat
     3661 cattcagcac attatacaac gagccaacaa ctccggtttt cggatctttg gctccatata
     3721 caacggttcc tattttactt aaaataatca ttcctgcgca catcaaacag ggttctaagg
     3781 ttacataaag cgtataatcg tataaaaacc cgggctctga agttaaaatt ttgtcaataa
     3841 ggagtttttc acaatgcgct aaaggattgg ctaactgccg gctacgatta tgttccttta
     3901 atattatagt gttattttta accagcagtg cacccacagg tatttcatct tcggtaaaag
     3961 cttttttcgc ttccgctatt gcttcctgca taaaaagata atgattttgg gaagaaaagc
     4021 aaatcatggt ttagtattga taactacctt tgctttttta acttgctcct ccatccagga
     4081 attaaaccaa tcctcaggta agttttgcgt ataaatggtt ttaatgcgtt ccggactgat
     4141 ttttgttttt tgcggacgaa aagctgttac tttgggaact ataataactt caccctcagt
     4201 tatttgcgga ggatttgcct tttccagata tgctttaact gcctgataat atatcctggt
     4261 aagtggtttt ccctgccaaa gtgaatctat ggttacttgt tccagttttt cccattgata
     4321 atttgcaggt atattctgat tgataagcca ccgctctaca atctgtttat tttggtcttt
     4381 tctgcgttcc ttacttaata tttgcacgat ttgcggtttt acttcttcca agggaagata
     4441 ctctttactt tgtttttcca ctacttcaaa gataagccag gcagataact ctgcagaata
     4501 taagggctct ataatatctc cgctattagc aatttccaga tatttcatca attttccttt
     4561 aatatcatta gcaggaacaa ttaaggaatc cgggctcata gcgggagtta aagtataact
     4621 aatgccaaat tcatctgctg cttgctgaag gcctatattt aatgccagtt tcataatttg
     4681 ttgcgcttgt gtttccggag ctaataaact tgctgtttta ggcaatgctt gcacaaaaat
     4741 ggtattatac agagtaaggg tcttggtgct ctgtattttt tgatacataa tccagccctt
     4801 ttcataaggc aggggttcac tgcaccttcc attctccagt cctgacagag cattttgaac
     4861 ttcccaaggt aattcttccg ttttttgata gccgttatca ataaaactta ccagagaatt
     4921 tttgttttcc agattagcaa taatatcctt aatttcaatt ccccgggcaa gttcattccg
     4981 aataaattca gcagtatttt tggcatcaga gagttctgtg ctgtccggaa taacaggaat
     5041 ggaacaatag cccaaacgat aaaactgcca taaacgaaat tgctctaaat ttgtttgata
     5101 ccaggcactg atttctgcat cagataaaga aatttctaat tgggagggag caaaaatata
     5161 gagctccaaa tctgcattac tggcaataat ctgttctacc tgtttgcgga ttttggaact
     5221 gagcatttcc tcttctatca acactttttg cagttttaga ataggaataa cattttcctg
     5281 atagtgtttt cttaaggcag ctaaattttc cggttgatcg gtcatcagcg attgtagata
     5341 caactttttg tcaaatttgc cattaacctg aaagcgaggg gatttgacta tatgctcggg
     5401 aatggaatta gaaagtgtat ccagaacttc tgcaacactg gcagaaatgt tatacttttt
     5461 ataataatcc tgcagaatta ttgctttagt gatatcacgc caggtctcat tatgcaattt
     5521 ttccttttct tcctttgtgg gagtgcgtcc tgtctgataa ccaaaaatag cataatgccc
     5581 acggtaggaa ttaaaaaaat cctccagaga aatctctgtt ccattcacgc ttcctgccac
     5641 ggaagaggaa tttgtagcgc aggaaacaag gaaaaataaa cccagcaggg taataacaat
     5701 aaatgtaaaa agcttgttca tttttctcct aaaagttgat tttgaatctt ttgcagatat
     5761 tgaaatgcct ctataggaga aagtttattc aaatcaatct ccttgatttc atttattata
     5821 ggatcgtttt cactggcttt atcagctaaa atctcaaaaa tatcaatctg aggaacatcc
     5881 cgcactaatt tcttacggat ggttgcagtt aaaccctgag gactgatttc atgctcttcc
     5941 aggtttttta agatttcttt ggctcggcgg ataacctttt cgggaattcc tgctaagcgg
     6001 gcaacctgta ttccgtaact ctggtctgcc ccaccgcgtt caattttacg aataaaaatc
     6061 atttcctcat tccactgctt tacagctaca ttgtaatttt ttatatccgg ataaagattt
     6121 tccagttctg ttagctcatg ataatgagta gcaaaaaggg ttaaagaatg tttgtatttt
     6181 tgaatatatt caataatagc ccaagctaag gataagccat caaaagtaga agttcctctg
     6241 ccaatttcat ccagtaaaat aagggaattg gaagtggcag aatgcaggat attagcggtt
     6301 tctatcattt ccactaaaaa agtgctttga ccttgagcca gattatcgga agctccaact
     6361 ctggtaaaaa cacgatcaaa aacaggaagg gtcattttac ttgccggcac aaaacttccc
     6421 atttgagcta aaataaccag caaaccaact tgtcttaaat aggttgattt tcctgccata
     6481 ttgggtcctg taataatagc aatactggtt tcaggataat ccagatgagt gtcattcgga
     6541 atgaatttat cgctttccat cagtttctca ataacgggat gcctgccttc tgtaatatgc
     6601 aattcccggc tttcagtaaa aacgggacgg gaatattgat tctgccaggc aagaaatgct
     6661 aaagaagaaa gaacatccaa ttcagcaata acttcgctca attgttgaaa acgaggcaat
     6721 gattcagcta agttttgacg cagttcttta aatagttcat attctaaatt tttaatcttc
     6781 tcttccgaag aaagcacttt tgcctcaaat tcctttaagc gtggtgaaat aaagcgttca
     6841 ctattggtca atgtctgttt gggaatataa taatccggca ctttattttt gttggcagaa
     6901 ctcacttcta tataatagcc aaaaacacga ttatagccaa ctttcaaatt attaataccc
     6961 gttttccggc gttcatcttc ttccaaacga gctatccagc ttttaccgtc ataaataata
     7021 tccatcagct catccagttc cggattatag cccttggcaa aaattccgcc ttcggttata
     7081 gaaacaggtg gattatcgtt aatagctttt tcaatgagtg ctataatatc ctcaaaacta
     7141 cccatattct gttgccagac agcaaattgg ggatgttcaa aagtgcttag tttggtttgc
     7201 aggtttctgg ctgaatacaa ataggacttt aaagcaataa gttctctggg gtttattctt
     7261 aaagaaccca aacgggtaac caaacgggta atatctccaa tttcctttaa gattaaacgc
     7321 agttctttta aatggctgct tttatcaata aaggactgaa tgatatcctg cctaaaagtg
     7381 atttccttga tgtctaaaag aggatgcaac agccattgct gtaaaagccg cgaacccatt
     7441 ggagtcattg tttggtctat aaccgaaagt aaactgccat attttgtgcc atagcgtatt
     7501 gaacgaacca attccagatt tcttctgctg atttcatcca gctgcatata ttgggaaagt
     7561 gaataatatc ttaacgaaga aatatgattc aagggagaat tgtaaagtcc ttgcacataa
     7621 gctaatgctg cacctgctgc
//
LOCUS       DAOPFL010000077         7585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_12364_length_7585_cov_94.793227, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000077 DAOPFL010000000
VERSION     DAOPFL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12364_length_7585_cov_94.793227"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1128)
                     /locus_tag="PLG69_05855"
     CDS             complement(175..1128)
                     /locus_tag="PLG69_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPI43069.1"
                     /translation="MKRLHLIYITMILCVPLIAGSFNWYSQNDERWKSEQLGSGKRSI
                     GSSGCVLSCLSMLLNAEASNEYMTPDRLNEWLKKNGGYSGSNMRWQIPGEIDGSGLGL
                     ELVAQSTVRNDWDFLSNELEKGNKVIVKVAGRRSHWVLVIKQDGPKNKPSSYIINDPG
                     MKEYEERTLAYFGGFKSARSYSGNWLDEQAFDLDSEINVVPVSQDELFLYDVAKLPHP
                     ADVYVTLHNNLPVEISGYFILGLFDEEGNYLRTVDYEYATVEASGNYDLLYELPDVKP
                     LNEEHQDLKIIYSKYFSNMPSLTESIELINKGIRNYTNTTN"
     gene            complement(1226..3115)
                     /gene="alr"
                     /locus_tag="PLG69_05860"
     CDS             complement(1226..3115)
                     /gene="alr"
                     /locus_tag="PLG69_05860"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPI43070.1"
                     /translation="MLRRKIFERSWVEIDLSAFRTNLNILKSFLEPQQQFMMIVKADA
                     YGHGAREISKIACEEGAVYLGVANPEEGRLLRLQGCKEPVLILSPCLSNEINTILDND
                     LAVNVSSLDFARALNKTAKTKNKQAVVHLKVDTGMHRSGILDIEFIDFFNKIAKMDNL
                     LIEGVFTHFASAENDPDFSKQQEERFWNIIDNLPFTPTYLHINNSNALVNGLGKHSNL
                     VRLGIMAYGIQVPGNKDIALQPVMTFKTILSQIKHIAKGESVGYNRSWTAKKDCLYGI
                     LPIGYADGYDFMLSNCGVVLLDNKICNVIGRISMDMICVDLSPIENPAIGDVAILLGG
                     SALETRAENLVSKYGGNSYELLCQVGRRAKRYYFQNGKMIYSTPLSRRDFVPDDFSDS
                     KLNQIIESAIAQRLQSIEIGELIYREMLRDFFYYKDKDIHYRHNFQHQVIFSHSLNAG
                     YFNATTTLNFDKVLSNDYFMVACASSDEVLRRYFKRSDVEYRWLMDDSFELDSKNFIV
                     CLAKVDDLILQTTLHYINGCLEIRCSHPELKKKIGKRVHFTINTLTLYPCSSHQFSVF
                     ITELTRGVDISFQYPLTINTVDCVPFFSGQDKDPIIQRKNGEIRVSSKPDEWIFPISG
                     VVFAY"
     gene            complement(3119..4459)
                     /locus_tag="PLG69_05865"
     CDS             complement(3119..4459)
                     /locus_tag="PLG69_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPI43071.1"
                     /translation="MERKIFILDTNVLIHNPQALFSFEDNRVVLPIVVIEEIDQFKKG
                     VDEKSRNARQVGRYLDALRKKGKLQEGVPTENGGTIQVTVNKEITNTASELLFLDRND
                     NLIISTALYFKEKYPQSIVTLVSKDVNVRVKADCVGINAENFETDSIKYEEFFTGWEF
                     LDLEPEQYRELEEKGYINNNFGDFFPNQFVRVSVPDKPDQYKTLRYHYGRNQLYAINH
                     YTGQQVFGIKARNFEQEMALDILLDDSIKLVSLSGKAGTGKTLLAMAAGLQKVVEEKR
                     YSRLVVSRPISPLGKDLGYLPGTKTEKFNPWMQPIYDNMDILLSSHTDKSQEGSSSRK
                     KTDINDLMDYGFLELEPLTYIRGRSLPDQFFIIDEAQNLSPHEMKTIITRAGENTKVV
                     LTGDPYQIDIPYLDSQSNGLSVSVEKFKGEILVGHITLDKGERSALADLAAKYL"
     gene            complement(4656..4743)
                     /locus_tag="PLG69_05870"
     tRNA            complement(4656..4743)
                     /locus_tag="PLG69_05870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4707..4709),aa:Leu,seq:cag)
     gene            5063..6397
                     /locus_tag="PLG69_05875"
     CDS             5063..6397
                     /locus_tag="PLG69_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPI43072.1"
                     /translation="MKRKVIIMGAAGRDFHNFNVFFRDNKDYEVVAFTATQIPGIDDK
                     KYPSALAGKLYPEGINIYPESELKNLIKKHKVDLVVLAYSDLPFEVVMHKASLVNACG
                     ADFMLMGKDNTTIKTSKPLVTVCAVRTGCGKSQTTRAVVKALKAKGLKVVSIRHPMPY
                     GDLVKQKVQRFAKLEDLKKYNCTIEEMEEYEPHIMMNSVIYAGVDYEAILKEAEKEAD
                     VIVWDGGNNDIPFYTSDKEIKIVVVDPHRPGDEISYYPGETNVFLADVIVINKIDSAD
                     FDDINEVRENVRMINPKAQIIEAASPLFVDHPEMIYGKKVLVVEDGPTLTHGEMTYGA
                     GIVAAEKYGCADLVDPRPWAAGEIKQTYEKYPEIGTLLPAMGYSSKQIKDLEKTINSA
                     ECDSVIIATPIDLRRLIKINKPACQVRYELQEIGVPTIADVLKNFGKKSSKK"
     gene            6540..7484
                     /gene="arcC"
                     /locus_tag="PLG69_05880"
     CDS             6540..7484
                     /gene="arcC"
                     /locus_tag="PLG69_05880"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPI43073.1"
                     /translation="MNKTAVLALGGNAIIKAGEKGTITQQFANTRESLSGIVELIKRG
                     YHLSITHGNGPQVGNLLRQQEAGEKEGIPQLPLGVLNAATEGTMGYMIEQSLQNKLHK
                     SGIKKRVITIISQVVVDKNDPSMLNPTKFVGSTYYTAQQAEELHQKLGWILKEDSGKG
                     FRRVVPSPYPIQIIPAATIKELVDRGEIVIAVGGGGIPIYIEEDGTYEGVDAVIDKDF
                     ASSLLALEIKADLFVILTGVEKVAINYGKENQQDLDRMTVAEAKRYFAEKQFPAGSMG
                     PKIQAAIDFLERGGSEVLITSIEKIVDAFEGKTGTRIV"
BASE COUNT         2385 a         1418 c         1443 g         2339 t
ORIGIN      
        1 tgataaaaaa ctttgatttg gtttgataaa aggaattttt tctctttagt agctatattt
       61 tttcgccggg ttaaaacctg ttgttagtat ctgtcgtttc tacggaattt ttactacaat
      121 atggttaaaa tgagggaaat gataataacc attatttcta ctgtattatt tggtttaatt
      181 ggtagtattg gtatagtttc taatgccttt attgatgagt tcaatggatt cagttaggga
      241 aggcatattt gagaagtatt tactatagat aatctttagg tcttgatgtt cttcattcaa
      301 aggtttaaca tccggaagtt catagagtaa atcataattt ccactggctt caacggttgc
      361 atattcatag tccacagtgc gcagatagtt tccttcttca tcaaagagac ccagaataaa
      421 atagccggaa atttctactg gtaaattgtt atgcaaagta acataaacat cggcaggatg
      481 aggcaatttg gcaacatcgt aaagaaaaag ttcatcttgc gaaacaggaa ctacattaat
      541 ttcactatcc aaatcaaacg cttgttcatc caaccagttt ccggaatagg aacgagcgga
      601 cttaaaccca ccaaaatagg cgagggttct ttcttcgtat tctttcattc cggggtcatt
      661 gataatatag gaagagggct tatttttagg accgtcctgt ttgattacca gaacccaatg
      721 acttcttctg ccggcaactt ttacaataac tttgtttcct ttctccaatt cattggaaag
      781 aaaatcccaa tcatttctaa cagtgctttg ggcaacaagc tccagtccta atccgctgcc
      841 gtcaatttca ccgggaattt gccaacgcat attacttccg gaatagccgc catttttttt
      901 cagccattca tttaaacggt caggagtcat atattcattg cttgcttcgg cgtttaaaag
      961 catagaaagg cagcttagta cacaaccgct gctacctatt gatctttttc ctgaacccaa
     1021 ttgttctgat ttccatcgtt catcattctg tgaataccag ttaaagctac ctgcaatcag
     1081 tggcacacac aaaatcattg tgatatatat taaatgcaat ctcttcataa gcaagtcacc
     1141 ttagctaaca actacaacaa tatagaaatt aactttaaga aaccactatt ctggagaggg
     1201 aaatatgtca aacaattttt tgttttcaat aagcaaaaac aactccacta ataggaaata
     1261 tccattcatc tggtttactg gaaactctta tttctccgtt ctttctttga atgatagggt
     1321 ctttatcctg accggagaaa aaaggtacac agtccacagt atttatagtc agtggatact
     1381 ggaaggaaat atccactccg cgagtaagtt ccgtgataaa aacactaaat tgatgcgaag
     1441 aacaaggata taaagtaaga gtattgatgg tgaaatgaac tctctttcct atttttttct
     1501 tcaattcggg atgggaacat ctaatttcca aacagccatt tatataatgc aaagtggttt
     1561 gcaaaataag atcatctact tttgccagac aaacgataaa gtttttacta tccagttcaa
     1621 agctatcatc catcagccaa cggtattcta catcgctcct tttaaaatag cgacgcagaa
     1681 cttcatcgga agaggcacaa gcaaccataa aatagtcgtt actaagcact ttatcaaaat
     1741 tgagggtagt tgtagcgtta aaatatccgg catttaaaga atgggaaaaa ataacctgat
     1801 gttggaagtt atgtctgtag tgaatatctt tatccttata ataaaagaaa tccctcagca
     1861 tttcgcgata aatgagctct cctatttcaa tgctctgtaa gcgttgagca attgctgatt
     1921 caattatttg attcaatttg ctatccgaaa aatcatcagg aacgaaatca cgccgggaaa
     1981 gaggagtgct ataaatcatt ttcccgtttt gaaaatagta tcttttagct ctacgtccta
     2041 cctgacagag cagttcataa gaattacctc catacttgga taccaaattt tctgctcttg
     2101 tttctaaagc cgaaccccct aaaagaatag ctacatcacc aatggcagga ttttctatcg
     2161 ggctcaaatc cacacaaatc atatccattg agatgcgacc tataacattg caaatcttat
     2221 tatccagcaa aacaacgcca caattggaaa gcataaaatc gtagccatca gcatagccga
     2281 ttggtagaat tccatataaa cagtcctttt ttgctgtcca ggaacgattg tatcccaccg
     2341 attcaccttt agcaatatgt tttatttgcg aaagtattgt tttgaatgtc ataacaggtt
     2401 gcaaagcgat gtctttattc ccgggaactt gaattccata agccataatt cccaaacgga
     2461 ctaaattact gtgttttccc aaaccgttta ccagggcatt ggaattatta atatggagat
     2521 atgtgggagt aaaaggcagg ttatctatta tgttccagaa tcgttcttcc tgttgtttac
     2581 tgaaatccgg atcgttttcc gcagaggcaa aatgagtaaa tacaccttca atcagcaggt
     2641 tgtccatttt agctattttg ttaaaaaaat ctataaattc gatatctaaa attccactgc
     2701 gatgcatacc agtatcaact ttcagatgaa caactgcctg tttgtttttt gtcttagcgg
     2761 ttttatttaa cgctctggca aaatccagag aagagacatt aacagccagg tcattatcca
     2821 aaatggtatt aatttcgtta cttaaacaag gagaaagaat taaaacaggt tccttgcaac
     2881 cttgcagtcg taataaacga ccctcttcag gattggcaac tcccaaatag acagctcctt
     2941 cctcacaggc aattttactt atttccctgg ctccatgacc ataagcatcc gctttcacaa
     3001 tcatcataaa ttgttgttgc ggttccagaa agcttttcag gatattcaga ttggttctga
     3061 aagcggaaag gtcaatttcc acccaactac gctcaaaaat ctttcttcgt aacatagttt
     3121 ataaatactt ggcagccaaa tctgccagag cactgcgttc acctttatct aaagttatat
     3181 gccctaccag aatttctcct ttgaattttt ctaccgaaac agaaagaccg ttactttggg
     3241 aatccaaata gggaatatct atttgatagg ggtctcctgt aagaacaact ttggtgtttt
     3301 ctcccgcacg ggtaataatg gttttcattt catgcggact caaattctgg gcttcatcaa
     3361 taataaagaa ttgatcgggc aaactgcgtc cccggatata agttagcggt tccagttcca
     3421 gaaaaccgta atccattaaa tcattaatat cggttttttt tctgcttgaa gaaccttcct
     3481 ggcttttatc cgtatgcgaa gagagcaaaa tatccatatt atcataaatg ggctgcatcc
     3541 agggattgaa tttttcggtt ttagtaccgg gtaaatagcc caggtctttt cccaaaggag
     3601 aaattggacg ggaaactaca aggcgtgaat atcttttttc ttccaccacc ttttgtaagc
     3661 cggcagccat agctaaaagt gtttttcctg ttcctgcctt gccggataag cttactaact
     3721 ttatactgtc atccaaaaga atatccagag ccatttcctg ttcaaaattg cgggctttaa
     3781 tgccaaaaac ctgttgtccc gtataatgat tgattgcata gagttgattg cgtccataat
     3841 gataccgcaa ggtcttatat tgatcgggct tatcaggaac agaaacacgc acaaactgat
     3901 tggggaaaaa atcaccgaag ttattgttta tatatccttt ttcttccagt tctctgtatt
     3961 gttccggttc caaatcaagg aattcccaac cggtaaaaaa ttcctcgtat ttaatgctat
     4021 cggtttcaaa attttctgca tttatgccta cacaatctgc cttaacacgc acatttacat
     4081 ctttggaaac aagagtaaca atggattgag gatatttttc cttgaaatac aaagcagtac
     4141 ttattattaa attgtcattt cggtcaagaa acaacagctc cgaagcggtg ttggttattt
     4201 ctttattcac tgttacttga attgtgcctc cattttctgt gggtacacct tcctgtaatt
     4261 ttcctttttt gcgtaaagca tcaagatagc gtccaacttg acgcgcgtta cggcttttct
     4321 catccacgcc ttttttaaac tggtcaattt cttctattac tacaataggc aagaccactc
     4381 tattatcctc aaaagaaaaa agtgcttggg gattgtgaat caggacattt gtatccagaa
     4441 taaaaatttt cctttccatt tgtataactt cctttgtttt tttatattta tcacgcagtt
     4501 tcgggtctgt tacccttctg aacagggttg gtatcttgaa tcaagaacaa taaaacaata
     4561 attagaatag ggcagggata gtcaatgatt attttcctga taatgactgc aaaaaaggat
     4621 tgatagaagc aaaaaacctg tagttaataa aaaaatggtg ctgaaggggg gatttgaacc
     4681 cccatgagct cattgctcac tagtccctga aactagcgtg tctaccaatt tcaccacttc
     4741 agcattgtat attaaatccc ttttcttaag aaagaaggat tttgtcaagg tttttcctac
     4801 aggtattttt tctgcagatt acgcaaatct tacgccgatt ttacagataa aagaacagaa
     4861 aacggatttt attttgtaaa ttgaaaaaga gataatttta caaagaggaa aagaggaaaa
     4921 gagaaaaaag aagaaggaat ttaaaaaggt tgagagagtt gaaatgctct ctctgccatt
     4981 ccagattttc cttgacggta aaacgctaat taaaacggtg aaaacaaata tgtaactata
     5041 ataaattaaa ggagattcat taatgaaacg caaagttatc attatgggtg cagcgggaag
     5101 ggactttcac aatttcaatg tttttttccg cgataacaag gactatgaag ttgtagcttt
     5161 tactgctaca cagattcctg gaattgacga caaaaagtat ccttccgctt tagcaggaaa
     5221 gctttatcct gaaggaatta atatttatcc ggaaagtgaa ctgaaaaact taattaagaa
     5281 acacaaagtt gatttggtgg tcttagctta cagtgacctt ccttttgagg tagttatgca
     5341 caaagcatct ttagtaaatg cttgtggtgc cgattttatg ctgatgggaa aagataacac
     5401 tactattaaa accagtaaac cccttgtaac tgtttgtgca gttagaaccg gttgcggtaa
     5461 atcacaaacg accagagcgg ttgtgaaagc tctcaaggca aaagggctga aagttgtttc
     5521 cattcgtcat cctatgcctt atggcgatct tgttaaacaa aaagttcagc gttttgccaa
     5581 actggaagac ctgaaaaaat ataactgcac aattgaagaa atggaagaat acgagccgca
     5641 tataatgatg aatagtgtta tttatgccgg agtggattat gaggcaattt taaaagaagc
     5701 ggaaaaagaa gcggatgtaa ttgtttggga tggaggtaat aatgatattc cattttatac
     5761 ttctgataaa gaaattaaga tagttgtggt tgatcctcat cgtcccggag atgaaatttc
     5821 ttactatccg ggtgaaacaa atgtcttttt ggctgatgta atcgttatca ataaaattga
     5881 tagtgccgat tttgatgata tcaatgaggt tcgcgaaaat gttcggatga tcaatcccaa
     5941 agctcaaatt attgaagctg cgtcaccgct ttttgtagac catccggaaa tgatttacgg
     6001 taaaaaagtt ctggttgtgg aagacggtcc tactttaacc catggagaaa tgacttatgg
     6061 agcaggaatt gtagctgcag aaaaatatgg ttgtgctgac cttgttgatc cgcgtccctg
     6121 ggctgcaggt gaaattaagc agacctacga aaaatatcct gaaatcggaa ctttgcttcc
     6181 ggcaatgggt tatagttcca agcagattaa ggatttggaa aaaacaatca atagtgctga
     6241 atgtgattct gtgattattg ccactccaat agacctgcgt cgtttaatta aaatcaataa
     6301 gcccgcttgt caagtgcgtt acgaattgca ggaaattgga gttcctacaa tagcggatgt
     6361 tctgaagaat ttcggtaaga aatcctctaa aaaataatag tttctataaa aggaggtgct
     6421 ttgtcgcctc ctttttccat ttgtgatcct ctggcagtaa agtttattca cttttttaca
     6481 ttaaaaggaa ttaacttttc tgcttcatca aaatttacat tagataaagg aaaaggaaaa
     6541 tgaacaaaac agctgttctt gcccttggtg gaaacgctat tatcaaagca ggtgaaaaag
     6601 gaaccataac tcagcaattt gccaatactc gtgaatccct tagtggaatc gtagaactaa
     6661 tcaaacgggg ctatcattta agcataactc atggcaacgg accccaggta ggaaatttat
     6721 tacgccaaca ggaagccggt gaaaaagaag gcattcccca attgccttta ggtgtattaa
     6781 acgctgctac ggaaggaact atgggatata tgatagaaca aagcttgcaa aataaactac
     6841 ataaaagcgg tattaaaaaa cgggttatta ctattatttc gcaggtagtt gtagataaaa
     6901 atgatcccag tatgctgaat ccgacgaaat ttgttggaag tacctattat acagctcagc
     6961 aggcagaaga attacatcag aaattgggtt ggatactgaa agaggattcc ggaaaaggtt
     7021 ttcggcgtgt tgttccttca ccttatccaa ttcaaattat tcccgctgca accatcaaag
     7081 aacttgtgga taggggagag atagtaatag cagttggtgg tggcggaatt cctatttata
     7141 tagaagaaga tggcacctac gagggtgttg atgcggttat tgataaggac tttgcttcct
     7201 cacttttagc tttggaaatt aaagcagacc tctttgtgat tttgacaggt gtggaaaagg
     7261 ttgctattaa ttacggtaaa gaaaatcagc aggacttgga tagaatgaca gttgctgaag
     7321 ccaaacgcta ttttgctgaa aaacaatttc ccgcgggaag tatgggtcct aaaattcagg
     7381 cagctattga ttttctggaa cgcggtggct cggaagtgtt gattacttcc attgaaaaaa
     7441 ttgtggatgc ctttgaaggc aaaaccggaa ccagaattgt gtagcttgag cttgccgtag
     7501 ctccgcatcc ttttttacga tttatcttcc ttagtagcat tgcttaccca ttgcttaccc
     7561 attacggatg gcattagcaa tgggt
//
LOCUS       DAOPFL010000078         7540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_12488_length_7540_cov_91.562592, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000078 DAOPFL010000000
VERSION     DAOPFL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12488_length_7540_cov_91.562592"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2970)
                     /locus_tag="PLG69_05885"
     CDS             complement(<1..2970)
                     /locus_tag="PLG69_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPI43074.1"
                     /translation="MSNFITNSGNNNLKSRLSELIRESQELKFLVGFFYFSGIQELID
                     SLQQNPDLILKILVGLNVDCLSYCIVEYAEERDKQNVERILLYHQSVLKAINNDIFDN
                     ELFYQQASYIIEQITAGRIIIRKTRKPNHSKLYLFKLKEEQIARKNLFITGSSNLTKA
                     GMTDQDEFNVEISDYGFDEAEQYFDTLWNNAISITEDDKEKTTLLNNLQYKTLLKSIH
                     PFDAYMYVVKSWLDTFYTEEYSSDIAYFMQKKGYRPLKYEIDAVWQGLSIIEENGGLI
                     LADVVGLGKSIIASLIGWKMRKRGIIICPPGLIGDDTANSGWKKYKEDFELYDWEVRS
                     GGDLDSIIEFLNRPKNIEVIIVDEAHRYRNGTTETYEKLKTICRGRKVILLTATPFNN
                     TPADILSLLSLFIVPKKSNITLNINLDDIFRSYNNLFKDLAFITKNWKSKDPSKKNDA
                     QRKYNAHFGIGEIDIKKVTDRVKLLSQQIREVIEPVTVRRNRLDLLYDPEYKEEIKEL
                     SKVENPKEWYYELTQEQSAFYDRVIEEYFTDNQDFLYRFKGPVYKPYKYEGIKTEEGK
                     LTRQEQIDQLSQDNLYDILRRQMVKRLESSFGAFKKSVENLLNSYIQIKTFIQNSNGR
                     YILDRKLINKADADDPESIDAILDQFMQNNLDKEGRKNKIYDVNKFTLKEQFFADIDS
                     DIALFQKILKEIDTLKLVENDPKLNTLLINVGKILKSAHPKNEPPAKVVIFSEYADTV
                     KHLESSLQEHFKNRVLVVTGGLSGNILEEINTNFDAAYTNQNDDYDILLSTDKLSEGF
                     NLNRASTVINYDIPWNPVRVIQRVGRINRISKKVFDHLYIINYFPTEQGADIVKSREI
                     AGQKMFMIHQTLGEDTRIFDPDEEPSPAKLFTKLQTNPYELEPESLYTKLKNLMHNWQ
                     EQFPERVSALNDMPIRIKTAKPYSSNSLVMCIRKGKLFCVSQDFTDEKGHYLSLPFEE
                     VLKRIECTPETEHLKLSP"
     assembly_gap    3194..3293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3299..4675)
                     /locus_tag="PLG69_05890"
     CDS             complement(3299..4675)
                     /locus_tag="PLG69_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPI43075.1"
                     /translation="MSKINLEKKWVFDPLHTVVIENHLAKLLFQADEGQEVNLEGIIN
                     FSHSQDTFQTEDYIQSEYQDGILKIILQDIDSDEVKDAVFTVTIPEGVYLKVKTDNYP
                     ISLNNLKNKLKVLNENSPIYLQNCQGDMHLENENGLIRLSDCEGNIDAKLENGPLSAS
                     KISGQTLHLENENGPIKVRMASFTEVELYSENGPIFYETIPVENGNFQFKTENGSINL
                     VLPNNFDFTLEATTQWGRVKTSFDLPITFNDNIYTMINGEGTSQIKAISDNGTIKINA
                     ENRLNLDFVMNKLEQIKIALQKVNSEAEKQKVVEMVNKITTYINRLADSIKEEKIKEK
                     ITSATSKLKDLVVNFDFRETNDKVIKSVEDIGSQIQDAFKEGIKNIKESVDDLKKHRF
                     HTESVAAYVKKILDSPQIKPYLGGEHKKKEKENIADRSRIKILEMLEAGKITAEEAER
                     LLKAIGKE"
     gene            complement(4672..>4977)
                     /locus_tag="PLG69_05895"
     CDS             complement(4672..>4977)
                     /locus_tag="PLG69_05895"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HPI43076.1"
                     /translation="HCPHCEISFQGDFENNWLAELDAGQLEFIRLFLLVQGNLKELQN
                     QLGISYPTVKNRLAEIIGIITKKEPVTDTVIDVLSDLEEGFINVDEAINMITQRREK"
     assembly_gap    4978..5077
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5228..5800
                     /locus_tag="PLG69_05900"
     CDS             5228..5800
                     /locus_tag="PLG69_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPI43077.1"
                     /translation="MKLDNQLKKILLILLIVILSQPLFCNTTADKTVAWLKAKGVAPE
                     LIVVIISMIPVVELRGSIPVAILLFNIPWLEAAVLSIIGNMIPVPFLLLLIDWFLGLI
                     SKVKPGRKFTEWLFTRTRRKGKSIEKYEEIGLVIFVGIPLPGTGGWTGALAANIFGLR
                     FWRSMLFIFFGVIMAAIIVTALSLMGTLAL"
     gene            5797..6942
                     /gene="lpxB"
                     /locus_tag="PLG69_05905"
     CDS             5797..6942
                     /gene="lpxB"
                     /locus_tag="PLG69_05905"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPI43078.1"
                     /translation="MIRKESYKIFWLVGESSADLHSELVMKALNDKFGNLTHIGIGGP
                     RMQRQGLKTLFSFEKFAVMGFVEVVKHLFFFLKVQRKLGKLFSTEKFDLAILVDYPGL
                     NLRIAKMADEMRIPVLYFICPQFWAWKHKRVYQLKDSVRYVACILPFEEELLKIHNIN
                     CSYVGHPIAEEITFELDRGSFARFFGLDPNKKWIGFFPGSRNNEITKMLPVFLQTAQK
                     WNPTEYQMLFSKSHSVNHQLYMHLIEGHKNIKLIDGYNYEMMKYCELLICTSGTVTLE
                     AAYIGTPLVICYKGSYLSYLIGRIFVRIKHIGLPNIVLDADLLPELIQGEMKPDNIYK
                     AGMQILSDPEKNAQIRKELFKLRAMLSDKHPSQEMPKIVRYLLDTYG"
     gene            6935..>7540
                     /locus_tag="PLG69_05910"
     CDS             6935..>7540
                     /locus_tag="PLG69_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPI43079.1"
                     /translation="MAKVLFYLERKLGAWLLLLLRKTLKFKVINQESSDNIRCIYMFW
                     HRNLLMMTLQRVYHGAAVMVSSSKDGELIAGPLWELGYIPVRGSSSFKGSSAMKEMIK
                     ISQKISLAITPDGPKGPCYTFHPGLFQIAYLAKIPIVAVAVNADKEWQFNSWDRFRFP
                     KPFAKLTMIYSDPIWVKNKEDFATAEREIRDFLAKNEKDTFF"
BASE COUNT         2165 a         1316 c         1283 g         2576 t
ORIGIN      
        1 cgggcttaat ttaagatgtt cagtttccgg agtgcattca attcttttca ggacttcttc
       61 aaaaggtaaa gacagataat gacctttttc atctgtaaaa tcctggctaa cacagaaaag
      121 tttgcctttg cgaatacaca taaccaagga attggaacta tagggtttag cggttttaat
      181 tctgataggc atatcgttta aggcagaaac tctttccgga aattgttctt gccagttatg
      241 catcagattt tttaattttg tatataaact ttccggttcc agttcataag gattggtttg
      301 taatttagta aatagtttgg cagggctggg ttcttcgtcc ggatcaaaaa tacgcgtatc
      361 ctctcccagg gtctgatgaa tcataaacat tttttgtcct gctatttctc tgcttttaac
      421 aatatccgca ccttgttcag taggaaagta attgataata tacaagtgat caaagacctt
      481 tttacttatt ctatttattc tgcctaccct ttgaataaca cgcacaggat tccagggaat
      541 atcataattg ataacagtgg aagcacgatt gaggttaaaa ccctcggata atttatcggt
      601 agaaagaaga atatcataat catcattttg gtttgtatag gcagcatcaa aattggtatt
      661 tatttcctcc agtatatttc ctgataatcc tcctgttaca actaaaactc tgtttttaaa
      721 atgttcttgc agtgatgatt caagatgttt aacagtatcg gcatattcgg aaaaaataac
      781 aactttggca ggaggttcat ttttcggatg ggctgatttt aatatctttc ccacattgat
      841 tagcaaggta ttcagtttgg ggtcgttctc taccagcttc aatgtatcaa tttcttttag
      901 tattttttgg aacagagcaa tatcggaatc tatatcggca aagaactgtt cttttaaggt
      961 aaatttatta acatcataaa tcttattctt tctgccttct ttatccagat tattttgcat
     1021 aaactggtct aaaatggcat caatgctttc aggatcatct gcatcggctt tattaattag
     1081 cttacgatca agaatatagc gtccatttga attttgaata aaggtcttaa tttgtatata
     1141 agaattaagg agattttcta cggatttttt aaaggcaccg aaagagcttt ccaaacgttt
     1201 taccatttgg cgtcttaaga tatcatacaa attatcctga cttaattgat ctatctgttc
     1261 ctgacgagtt aattttcctt cttcagtttt aatgccttca tacttatagg gcttataaac
     1321 aggacctttg aaacgataaa gaaaatcctg attatctgta aaatattctt caataaccct
     1381 gtcgtaaaaa gcggattgct cttgggttaa ttcataatac cattccttag ggttttccac
     1441 tttagagagc tctttaattt cttctttgta ttccggatca tataataaat ccaatctgtt
     1501 tctgcgaacg gtaaccggtt ctattacttc tctgatttgt tgtgagagta atttgactct
     1561 gtcggttact tttttaatat caatttcccc gataccaaaa tgagcattgt attttctttg
     1621 ggcatcattc tttttacttg ggtctttgga cttccagttt ttagttataa aagcaaggtc
     1681 tttaaataag ttgttgtaag agcggaaaat atcatccaaa ttaatgttga gagtgatatt
     1741 ggactttttg ggaacgataa ataaactaag caaagaaagt atgtcggcag gtgtgttatt
     1801 aaaaggagtt gccgtaagca gaataacttt gcgtccccga caaatggtct tcagcttttc
     1861 gtaggtctcg gtagtgccgt ttctataacg atgtgcttcg tcaacaatga tcacttcaat
     1921 atttttaggc cggttaagaa attcaataat gctatccaaa tcccctccgg aacgcacttc
     1981 ccaatcataa agttcaaaat cctctttata ttttttccat ccggaattgg cagtatcatc
     2041 tccaatcaaa ccgggagggc aaataataat tccccttttt ctcattttcc agccaattaa
     2101 agaggcaata attgatttac ccaagccaac aacatccgct aaaatcaatc ccccattttc
     2161 ttctatgatg gataaaccct gccaaacagc atcaatttca tattttaagg gtcggtaacc
     2221 tttcttctgc ataaaatagg caatatcgga actatattct tctgtgtaaa aagtatccag
     2281 ccaggatttc actacataca tataggcatc aaaaggatga atggatttaa gtaatgtttt
     2341 atattgcaga ttgtttagca gagttgtctt ttctttatca tcttctgtaa tagaaatagc
     2401 attattccac aaagtatcaa aatattgctc tgcttcgtca aaaccataat cggaaatttc
     2461 cacattgaat tcatcctgat cagtcatacc tgccttggtt aaattactgc tacctgtaat
     2521 aaataaattt ttacgagcta tttgttcttc tttcagtttg aatagataga gcttggagtg
     2581 attgggtttt cttgtttttc ggatgattat tctacctgct gtaatttgtt caatgatata
     2641 gcttgcctgc tgataaaata gttcattatc aaagatatcg ttattgatag ctttcagaac
     2701 ggactggtga tacaatagga ttcgttctac attctgttta tctctctctt ccgcatattc
     2761 tacaatacaa tagctaagac aatcaacatt taagccaacc aggattttta aaattaaatc
     2821 aggattttgt tgtaaactgt ctattaactc ttgtatgcca gaaaaataaa aaaaaccaac
     2881 cagaaatttc agctcctggc tttctcttat taattcagat agacgggatt ttaagttatt
     2941 attgcccgaa ttggtaataa aattactcat taaaactccc ttgcataaac ataatccgca
     3001 tagcttaaaa ttatgagaat aaatataaat actatatcct gcataaactg atttaacctg
     3061 aagattacta tgtgcataat ttatagggtg acaaaatcgg atttgtcgtc caaaaataat
     3121 aaaagatttt cactctttgt gcttttttac ctttacacta ttatattacc tattttagat
     3181 gaacagtaat aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttttttc
     3301 actctttccc aatagctttc aacagtcgtt cagcttcttc agcagtgatt tttcctgctt
     3361 ccagcatttc caagattttt atgcggctgc ggtctgcaat attctctttt tcttttttct
     3421 tgtgttcacc tcccaaataa ggtttaattt ggggtgaatc cagaattttt ttaacataag
     3481 cagctactga ttcggtatgg aaacgatgtt ttttgaggtc atctacactt tctttaatat
     3541 ttttaatacc ttctttaaag gcatcttgaa tctgggaacc gatatcttct acgcttttaa
     3601 ttaccttatc attagtttcg cgaaaatcaa agttcactac caaatccttt aatttgctgg
     3661 tagcagaggt tattttctct tttatttttt cttctttgat ggaatcggca aggcggttaa
     3721 tataggtggt aattttattt accatttcca caactttctg tttttctgct tccgaattta
     3781 ctttctgcaa ggcgatttta atctgttcca atttattcat cacaaagtcc aaattcaggc
     3841 gattttctgc attgatttta atggttccat tatcggaaat tgccttaatt tgcgaagttc
     3901 cttctccgtt aatcattgta tagatattgt cattaaatgt aatcggtaaa tcaaatgagg
     3961 ttttcactct gccccattgt gtagttgctt ccaaagtaaa atcaaaatta ttgggcagca
     4021 caagattaat tgagccattt tcggttttaa actggaaatt gccattttct acagggatgg
     4081 tttcgtagaa gataggtccg ttttcggaat ataattccac ttcggtaaaa gaagccatcc
     4141 gcacttttat cggtccgttt tcattttcca gatgaagggt ttgaccgcta attttggatg
     4201 ctgataaggg accgttctcc aatttagcat caatattacc ttcacaatca gacaaccgaa
     4261 tcaagccgtt ttcattttcc aaatgcatat cgccttgaca gttttgcaga tagatgggac
     4321 tattttcatt caaaacttta agcttgtttt tcaggttatt caaactgata ggatagttat
     4381 ctgtttttac tttcagataa acgccttctg gaatggttac agtaaacacg gcatctttaa
     4441 cttcatcaga atcaatatct tgtaggataa tctttaaaat cccgtcttga tattcggatt
     4501 gaatataatc ctcggtctgg aaggtatctt gagaatgact gaaattgatt atcccttcca
     4561 aattaacttc ttgtccttca tctgcttgaa ataataattt agccagatga ttttcaatta
     4621 ctactgtgtg taaggggtca aaaacccatt ttttctccaa attgattttg ctcatttttc
     4681 tctcctttga gtaatcatat ttattgcctc gtctacattg ataaaaccct cttccaaatc
     4741 ggataaaaca tcaataaccg tatccgtaac cggttctttt tttgttatga tcccgataat
     4801 ttctgctaaa cggtttttta ctgtgggata tgatattccc agctgatttt gcagctcttt
     4861 caaattacct tgcactaata aaaataaacg aataaactca agttgaccag cgtctaattc
     4921 tgccagccag ttattttcaa agtcgccttg aaaactaatt tcacaatgcg ggcagtgnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntat atgactcctg actttaatat
     5101 cggcagaact atgtcctcat tcgttttcta atcattggtt gacgagaaca tcaaccatcc
     5161 gtctatacaa attattgaca aaatacgggt ttttgataaa tggaattatg ataatgttat
     5221 aaaggaagtg aagttggata accagctaaa aaagatactg cttattctgt tgatagtaat
     5281 tttgtcccag ccgctttttt gtaatactac tgcagataag accgttgcct ggttgaaagc
     5341 aaaaggtgtt gcacccgaac tcattgtggt gataatttca atgattccag ttgtagaact
     5401 gcggggaagt atacctgtgg caattttatt atttaatatt ccctggttgg aagcggcagt
     5461 tctctccatt attggcaata tgattccagt ccctttccta ttgttgctga ttgattggtt
     5521 tttgggctta ataagtaaag tgaaaccagg tcgtaaattt actgaatggc tttttaccag
     5581 aacaagacgc aaaggtaagt ccatagaaaa atacgaggaa attggtttag ttatttttgt
     5641 tggcattcct cttcccggaa ccgggggttg gacgggtgct ttagctgcta atatatttgg
     5701 cttaagattt tggcgttcaa tgctttttat attttttggg gtaattatgg cagctattat
     5761 tgtaactgct ttatctttga tgggaacttt agctttatga tacgcaaaga aagttataaa
     5821 atattttggc tggtgggtga aagttctgct gatttgcatt ccgaactggt aatgaaagca
     5881 ttaaacgata aatttggtaa tttaacccat attggaattg gcggacccag aatgcaaagg
     5941 caaggtttga aaacactctt ttcatttgaa aaatttgctg tgatgggttt tgtagaagtg
     6001 gtgaaacatt tgttcttttt cctgaaagtc cagcgtaaat taggcaaact gttttccaca
     6061 gagaaatttg accttgccat tcttgttgat taccctggtt tgaatttaag aattgctaaa
     6121 atggcagatg aaatgcgtat tcctgtgcta tattttatat gtcctcaatt ttgggcttgg
     6181 aaacataaaa gagtatatca gctaaaggac tctgtgcgtt atgtagcttg cattcttcct
     6241 tttgaagagg aattactgaa aattcataat ataaattgca gttatgtagg tcaccctata
     6301 gcggaagaaa ttacttttga gcttgatcgt gggtcttttg cccgtttttt tggtcttgat
     6361 cctaataaaa aatggattgg ttttttccca ggaagccgaa ataatgaaat aacaaaaatg
     6421 ctcccggtct ttttacagac agctcaaaag tggaacccaa ctgaatatca aatgctattt
     6481 tccaaatcac atagcgttaa tcatcaactt tatatgcatc tgatagaagg acataagaat
     6541 atcaagctca ttgatggtta taactatgaa atgatgaaat attgtgaact gctgatctgc
     6601 acttcaggca ctgtaacttt ggaagctgcc tatattggaa ctcctctggt aatatgttac
     6661 aagggttctt atttatccta tctgataggt cggatatttg ttagaattaa acatatcggt
     6721 ttacccaata tcgttttgga tgcagacctc ctgccggaac tgattcaggg agaaatgaaa
     6781 ccggataata tttataaagc tggaatgcaa atcttaagcg atcctgaaaa gaatgcccaa
     6841 atccggaagg agctatttaa actgagagca atgctaagtg ataaacatcc ctctcaggaa
     6901 atgcctaaaa tagtccgtta tttacttgat acttatggct aaagttcttt tttatctgga
     6961 acggaaactg ggtgcctggc ttctgttgct tttacggaaa acactgaaat tcaaagtaat
     7021 aaatcaagaa tcctccgata acatacgctg catctatatg ttctggcata gaaatctttt
     7081 gatgatgact ctccaaagag tttatcacgg tgcagccgtt atggtttctt cttccaagga
     7141 tggagaatta attgccggac ctttatggga acttggatac attcctgttc ggggctcaag
     7201 ttcttttaag ggttcttcgg caatgaaaga aatgataaaa atatcccaaa aaatatccct
     7261 tgccattact cccgatgggc ctaaaggacc ttgctacact ttccatccag gtctttttca
     7321 aattgcttac ctggcaaaaa ttcctattgt agccgtagct gtaaatgctg ataaagaatg
     7381 gcagttcaat tcctgggaca gatttcggtt tcccaaacct tttgccaagc taactatgat
     7441 ctactccgat cctatctggg tgaaaaataa agaggatttt gcaactgcag aaagggaaat
     7501 acgggatttc ctggcaaaaa acgaaaagga tacttttttt
//
LOCUS       DAOPFL010000079         7481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_12623_length_7481_cov_103.351872, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000079 DAOPFL010000000
VERSION     DAOPFL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12623_length_7481_cov_103.351872"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLG69_05915"
     CDS             <1..804
                     /locus_tag="PLG69_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43080.1"
                     /translation="DYENFNHKLKEADVYLNLNGESRIVEVTDPKKIQIRNRMLEKGR
                     KIIKEKNIRLAMMPSLLNDTLSEQAIESEVQFWRALDIDYQMLSDKTLALCRKFVNKN
                     YVEIENKSAAFRFAINNIWAECGSFGKGEFQSPIINLPGGEILIIPKPGSMQGKIIGD
                     RAYALGEEILHPEVEIVNNEITGFSAESNQQQLAKAIETGGKDGRKVALICLGTNDNI
                     RGGNIDNALKHKSSGFISIYWGSNKDIGGDIAGNIEWFIQIENPQLNFL"
     gene            820..4080
                     /locus_tag="PLG69_05920"
     CDS             820..4080
                     /locus_tag="PLG69_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPI43081.1"
                     /translation="MKKSFLLCLLICCSAFAFGQWHIDEDFEGITTLPAGWITYDDGD
                     GMTWRNLENASHAHSGTRFAFADNYFPNQNCDWLITPQLHIANGDSLKFYTRAWFDTE
                     NLQVYVSTTGNAISNFNTQILNLQGLGTTFQYASYNLSAYAGQNIYIGFKWQCVTYGI
                     IVDDVKIGQPVIVTPELNLPESITFWQGETITVDFTPYIVATDINNIQLTWQTPEHIS
                     ISATGLALTFSSDNWNGTENITFTLTDNITGLTATDTMQIIVHPIPTVDMALQTILSP
                     GNMVFVNSAFNPTVTVKNNGQILWEDQLEIFFQVYNSQNVLVESVSSYFNPVLQPEAT
                     FEAVFPPLSISSEGVYTGTFQINISDNNPTNDTISCVFNVILRVNTGGPDAFGYRYID
                     STAENGPDFNWIDISATGTSTIMYGVNAFNGDDNFSEPIPLGFNFPFYGISYSTAYVD
                     INGEILLAPNTWYTEYPDNGWGNDGNMFNYMYPIPGYSAMPGLIAVYWDDLHCDQGTG
                     DIYFQSFGEEPNRYCVIQWHNLRFHAGTGVSTLLDFEVILQENGEIIMQYNSTATGQT
                     GANVPHDNGKSATVALQSADATMGICYLREIVQNNTYIGVEPAGNLLFNGLALRFYSG
                     EDTQAPFITHSAPGNTFNQSPLITARILDLSELNNVTLHYNLGEGWNALEGMATGNGN
                     YDFALPLLPTGHTFSYYFAAADVCGNSSTLPAGAPIEVFQFKILPSENTSVLLAYSGK
                     QDYQNTELPVYIEQLNNANIAYDIYDWEEFAEYTIPNVYSTILAYACTGTASPATLYL
                     SQKLMDFLNLGTIENPKNLFFSSDGFAFSQSGTPNSHPMKQLLNGYFRTFAVSTGGGG
                     GTNGLAGPDVFSYQSGTIMCTTYSPVGTIGTEYSVYANSPDCIFAYDEVPDWYADLVP
                     YPEIGAMNAFVFEDGPVEGHAYLYHGVCATAVQTPIFRTFYFSFDFSQLNNPAQREEL
                     FADLMDWFEVYPTSINENELPIVQSKLQTNYPNPFNPATTIAFTLAKADKVEIVIYNL
                     KGQKVKTLLSDNLLAGNHSIVWDGKDDKGKTLSSGIYLVKMKTGKTIDTRKITMLK"
     gene            4092..5774
                     /locus_tag="PLG69_05925"
     CDS             4092..5774
                     /locus_tag="PLG69_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43082.1"
                     /translation="MPKYLILTMLLFSLSVLTAGDYIIGTGTSNQNYVPFNGNYNYSW
                     SRFFFTADELLAAGMTGTVQITKIGFQLVSNTLDNYITNDQRIYIREFYDENYPSNPQ
                     YINPSSYPVAYQGTISWTSPGWVFIQLTTPYSYTYNGGSPVPNGIEILWENRDGSSQS
                     PYPKFAYTSTDENRAAYKYSNSSFPTGSGTRYNKRPNVWFISPATSEPNPAVNPIPAN
                     LATGVEITTTLKWTSGGGNPDDYLVSLYKTDPLTYIVNNQVTTSTTYNLPVRLEYSTT
                     YYWRVIPRNSFGSAIACPTWSFTTIPDPSIMTYPWTENFDGSEFPPTDDWLRRGGDLV
                     DPIQLGGNSLWEQENWLNISGTDKAAGINIWGNLNGWLITPQFLFNEDSDYLYFDLAL
                     LKYNQPPNGTPPDTTGIDDRFAVLISDGYTWSTANIMREWNNSGSPYVLNNISPWGER
                     VCIPLNGLTGHKRIAFFAGSTISNADNDFMINNLYVGPLIPDTPEVQISQVGDDLCLL
                     TWNESSGATGYDIYAADSPEGPWELIASTAETQFEFTAQAAKKFYRVKAIAR"
     gene            6007..6774
                     /locus_tag="PLG69_05930"
     CDS             6007..6774
                     /locus_tag="PLG69_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI43083.1"
                     /translation="MIVLKNVTCGYQDFPVLQDISLQIESGEFCALLGPNGAGKSTLL
                     YTIMGYLKPSKGFISIFGKEIASVKHSWLAKQLAFVPQETRSEFDHTVQETVLMGRYP
                     YLGLLQSYGSEDFDTVDSVLEELKLLELKHRWLSEISGGEKQRVLIARALVQQTPFIL
                     LDESLSQLDINYQIEIMKLLKAIHSKENKTVLIVSHNLNLAANYAERLIFLKEGKVLA
                     SGTPELLMQKETLKELFTIELDIMQNPHSGRPNIIYP"
     gene            6785..>7481
                     /locus_tag="PLG69_05935"
     CDS             6785..>7481
                     /locus_tag="PLG69_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPI43084.1"
                     /translation="MQFLRFFTVFFILLAFFAMLNGIKVTGIVTDEENKPLEAVRLIS
                     GKKTTLSKQNGNFTIEVTDSLQVSRLGYKKQVLSIQEVIALPKTASGIQIRLQSEPVQ
                     LSTYRVFAYLSEENISPADVVTLPIDPDKHYSSASELISQTASFQGSGIQLKGEIAEI
                     NILGNISRHTLVLLDGIAMNTLGEPFDFSRLNVDNIESIEVVKNNASVYGGGSAIGGI
                     IMITTKQGSALKKN"
BASE COUNT         2226 a         1444 c         1537 g         2274 t
ORIGIN      
        1 gattatgaaa attttaacca taaactgaaa gaagcggatg tttatttgaa ccttaatggt
       61 gaatcccgaa ttgtggaagt tactgatccg aaaaaaattc aaatccgtaa ccgaatgctg
      121 gagaaaggtc gtaagattat aaaggaaaag aatatccgtt tggcaatgat gccttctctt
      181 ttgaatgaca ctctttccga acaggcaatt gaaagtgaag ttcagttctg gcgtgcttta
      241 gatattgatt atcagatgct tagcgataaa accctggcgt tatgcagaaa gttcgttaac
      301 aagaattatg tggaaattga aaacaaatct gccgctttcc gttttgccat caataatatt
      361 tgggcagaat gcggttcttt tggcaaaggt gaatttcagt cccctattat taacctgcct
      421 ggtggtgaaa ttttaattat ccccaagccg ggttcaatgc agggaaaaat aattggtgac
      481 cgtgcttatg ctttgggcga agaaattttg catccggaag tggaaatcgt caataatgaa
      541 ataaccggtt tttctgcaga aagcaatcag cagcaattag ccaaagccat tgaaaccgga
      601 ggtaaagatg gcagaaaggt tgctctcatc tgtttaggaa ccaatgataa catccgtggc
      661 ggcaatatag ataatgcctt gaaacataaa agcagcggat ttataagtat ttactgggga
      721 agcaataaag atattggtgg agatattgct ggaaatattg aatggttcat ccagattgaa
      781 aatccccagc ttaactttct ataagaaaag aggtctatta tgaaaaaatc atttttactg
      841 tgtctcctca tttgctgttc tgcttttgct tttgggcaat ggcatataga tgaggatttt
      901 gagggtatta caacccttcc tgccggttgg attacttatg atgatggtga tggaatgacc
      961 tggcgtaatt tggaaaatgc cagtcatgcc cattccggaa cgcgttttgc ttttgcggat
     1021 aactattttc ccaatcaaaa ttgcgattgg ctgattactc ctcaattgca tattgccaat
     1081 ggagattcac tgaaatttta cactcgtgcc tggtttgaca ctgagaactt gcaggtttat
     1141 gtttcaacaa ccggcaatgc catcagcaat ttcaacactc aaatattgaa tttacagggc
     1201 ttgggaacta cttttcaata tgcaagttat aatctttccg cttatgcagg acagaatatt
     1261 tacatcggat ttaaatggca gtgtgttact tacggaatta ttgtagacga tgtgaaaata
     1321 ggtcagcccg taattgtaac tcccgaattg aacctgcctg agagtattac tttctggcag
     1381 ggggaaacca taaccgtaga ttttactccc tatattgtag ctacggatat aaacaatatt
     1441 cagcttacct ggcaaactcc tgaacatata agtatttctg ctacgggttt agctctaact
     1501 ttcagcagtg ataactggaa cggaacggaa aatatcactt ttactctaac ggataatata
     1561 accggtttaa cagctacgga tactatgcag ataattgtgc atccgattcc aactgtagat
     1621 atggcattgc aaacaatttt atcgcccgga aatatggttt ttgtaaactc tgcttttaac
     1681 cctaccgtta cggtaaaaaa taacggacag attttgtggg aagaccagct ggaaatattt
     1741 ttccaggtct ataatagcca gaatgttctt gtagaaagtg tttcttccta ttttaatcct
     1801 gttttacagc cagaggcaac ttttgaagct gtctttcccc ctctcagtat tagttccgaa
     1861 ggtgtttata ccggaacttt tcagattaac atcagcgata acaatcccac taatgatacc
     1921 atcagctgtg tattcaatgt tattttaaga gtaaatacag gcggacccga tgcttttggt
     1981 tatcgctata tagatagcac agcagaaaac ggtcctgatt tcaattggat tgatattagt
     2041 gctaccggca cttccactat tatgtatgga gtgaatgcct ttaacggcga cgataacttt
     2101 tccgaaccca ttccccttgg ctttaatttt cccttctatg gtattagcta ctctaccgct
     2161 tatgtagata ttaacggaga aatattattg gcaccaaata cctggtatac agaatatcct
     2221 gataacggtt ggggaaacga tggtaatatg tttaactata tgtatccgat tccaggttat
     2281 tctgcaatgc caggtttgat agcagtttac tgggatgacc tgcattgtga tcaaggcact
     2341 ggagatatct attttcaaag ctttggagaa gagcccaatc gttattgcgt aattcaatgg
     2401 cataatttga ggtttcatgc tggaaccggt gtttccactc tgctggattt tgaagttatt
     2461 ctgcaggaaa acggggaaat cattatgcaa tacaattcta cagcaacagg acaaacagga
     2521 gcaaatgtac ctcacgataa tggaaaaagc gctaccgttg ccttacaaag tgcagatgct
     2581 acaatgggaa tttgttattt gcgggaaatt gtgcaaaata atacttatat cggagttgaa
     2641 ccagcgggaa atttactttt caatggatta gccctgcgtt tttatagcgg tgaagacaca
     2701 caagcgcctt ttattacaca ttctgctccg ggaaacactt ttaatcagtc acctctgata
     2761 acggcaagaa tattagacct ttccgagctg aataatgtaa ctttgcatta taatttgggt
     2821 gagggctgga atgctttgga aggtatggca acaggtaatg gcaactatga ttttgctttg
     2881 ccattacttc ctacagggca tacctttagc tattattttg ctgctgctga tgtctgtggc
     2941 aatagttcta ctttgcctgc aggagctcca atagaggtct ttcaattcaa aattctgcct
     3001 tcggaaaata cttctgtctt gcttgcctac agcggaaagc aggattatca aaataccgaa
     3061 ctgccagttt atatagaaca actcaataat gccaatattg cctacgacat ttatgattgg
     3121 gaagaatttg ccgaatatac aattccgaat gtctattcta ctattctggc ttatgcctgc
     3181 acaggcacag caagtcctgc taccttgtat ttatcacaga aattgatgga ctttctgaat
     3241 ttgggcacta tagaaaatcc taaaaacctg tttttctcct ccgatggctt tgctttcagc
     3301 caatcgggta ctcctaattc ccatcctatg aagcaattgc taaatggtta tttccgaact
     3361 tttgctgttt ctacaggtgg tggaggcgga actaatggtt tagcaggacc tgatgttttc
     3421 agctatcagt caggcacaat tatgtgcaca acttattctc ctgtaggaac tatcggcaca
     3481 gaatacagcg tttatgccaa tagcccggat tgtatttttg cctatgatga agttccagat
     3541 tggtatgcag accttgttcc ctatccggaa atcggagcga tgaatgcttt tgtgtttgaa
     3601 gacggacctg tagaaggtca tgcctattta tatcacgggg tttgtgctac tgctgtgcaa
     3661 acacctatat ttaggacttt ttacttttcc tttgattttt cccagctgaa taatcctgct
     3721 caaagagaag aactgtttgc cgatttaatg gactggtttg aggtttatcc cacttccatt
     3781 aacgaaaatg aactgccgat tgtgcaaagc aaacttcaaa caaactatcc gaatccattt
     3841 aatcccgcaa cgacaattgc ttttactttg gcaaaagcgg ataaagtgga aatagttatc
     3901 tataacctaa agggacaaaa agtaaaaacc ctgctttccg ataatctgct tgcgggtaac
     3961 catagtattg tttgggacgg taaagacgat aaaggcaaaa ccttaagcag tggaatttat
     4021 ctggtgaaaa tgaaaaccgg aaaaaccata gacacccgca aaatcacgat gctcaaataa
     4081 ggagtaaaaa aatgcccaag tatctaattc taactatgct gctgttttct ctgtctgttc
     4141 tcacagcagg agattacatt attggcactg ggacaagtaa tcaaaactat gtccctttta
     4201 acggaaatta caactatagc tggagcaggt tctttttcac ggcggatgaa ttattggctg
     4261 ccggaatgac gggaaccgta caaataacca aaatcggttt tcagctcgtt tctaacaccc
     4321 ttgataatta tattaccaac gaccaacgga tttatatccg cgagttttat gacgaaaatt
     4381 atccttccaa tcctcaatat attaatccct cctcttatcc ggttgcttat caaggaacta
     4441 tttcctggac aagtcccggt tgggttttta ttcagctaac gacaccctat tcttatactt
     4501 ataacggcgg gagccctgta cccaatggaa ttgaaattct ctgggaaaat agagacggct
     4561 cttctcaaag cccctatccg aaattcgctt atacttccac tgatgaaaat agagccgcct
     4621 ataaatatag caattcttct ttcccaaccg gttccggtac cagatataac aaaagaccta
     4681 atgtctggtt tatttctcct gctacatcag agcccaatcc ggcagttaat cctattccgg
     4741 caaatttggc tacgggagtg gagataacta caaccctgaa atggaccagc ggaggaggta
     4801 atccggacga ttatcttgtt tccttgtata aaaccgatcc gctaacatac attgtaaata
     4861 atcaggtaac tacctccact acctataatt tacctgtgcg tctggaatat agtacaactt
     4921 actactggag agttatcccc cgtaatagtt ttggatcagc tatagcttgt cctacctgga
     4981 gttttactac tattccagac ccttcaataa tgacttatcc ctggacggag aattttgacg
     5041 ggagcgaatt tcctccaact gatgattggt tacgcagagg aggtgatctt gttgacccta
     5101 ttcaattggg aggaaattct ttatgggaac aggaaaattg gcttaacatt tccggaacgg
     5161 ataaagccgc cggcattaat atctggggta atttaaacgg ttggctgatc acacctcaat
     5221 ttctgtttaa tgaagatagt gattatcttt actttgattt ggcattgctg aaatataatc
     5281 aacccccgaa tggaactcct ccggatacaa ccggtattga tgatcgtttt gccgttttaa
     5341 tttccgatgg ctacacctgg agcacagcca atattatgcg cgaatggaat aatagcggtt
     5401 ctccttatgt gttaaataat attagccctt ggggagaaag ggtttgtatt cccttaaacg
     5461 gtttaaccgg acataaaaga attgcctttt ttgccggctc tacaatttct aatgccgata
     5521 acgattttat gatcaataac ctttatgtgg gtcctttaat tcccgataca ccagaagtgc
     5581 aaattagcca agttggagat gatctttgtc ttctaacctg gaatgaaagt tcaggagcta
     5641 ccggttatga tatctatgct gctgattcac ctgaaggtcc ttgggagcta attgcttcca
     5701 cagcagagac gcaatttgag tttacagccc aggcagctaa aaaattctat cgggtgaaag
     5761 caatcgcaag atagagtaaa cgatattcca atcgtttctc ttaaagtaaa aaaagtgtag
     5821 gcggaggaca ctgtttctcc gcaaagtaat aaaacgcaac aggaggttga cctggaggtt
     5881 aacctcctgt gtagttgacc gggaggtcaa ttacccggtt gaaaacggtg aaagttaagt
     5941 tgacaatttg gcttccaata aaaatcctgt tcaaagaaga atagcattat acggattatt
     6001 agcacgatga ttgttttaaa gaatgtaacc tgcggttatc aggattttcc tgttttgcaa
     6061 gatatttccc tgcaaattga aagcggggaa ttttgtgctc tattaggtcc taacggagcc
     6121 gggaaatcta ccttacttta taccattatg ggttatttga aaccgtcaaa gggttttatt
     6181 tccatattcg gcaaggagat agcaagcgta aaacactcct ggttggcaaa acaactggct
     6241 tttgtgcctc aagaaaccag gtctgaattt gaccatacag ttcaggaaac ggttttaatg
     6301 ggtcgttatc cttatctggg tttattgcag tcctatggtt ccgaggattt tgataccgtt
     6361 gattctgttt tggaagagct gaaactattg gagctgaaac accgctggct ttctgaaatc
     6421 agcggaggtg aaaaacagag ggttttaatt gccagagcat tagttcagca gacaccgttt
     6481 attttgctgg acgaaagttt gtcccaattg gatattaatt atcagataga aataatgaaa
     6541 ctgctaaaag ccattcacag caaagaaaat aagacggttt taatcgtttc gcataactta
     6601 aacttggcag ccaactatgc cgaaaggtta attttcctga aagagggcaa ggttctggct
     6661 tccggaacac cggaactttt aatgcaaaaa gagaccctga aagagttatt tacaatagaa
     6721 ttggatataa tgcaaaatcc gcatagcgga cgccctaata ttatttatcc ttgagtgttt
     6781 tattatgcag tttctccgtt tttttaccgt tttctttatt ctgcttgcct tttttgcaat
     6841 gctaaatgga ataaaagtaa ccggaattgt tacggatgaa gaaaataaac ccctggaagc
     6901 ggttcgcctg attagcggaa aaaaaaccac cttaagtaaa cagaacggca attttaccat
     6961 tgaagttacc gactctttac aagtaagtcg tttgggctat aaaaagcagg tgctatctat
     7021 acaggaagtt attgctctgc ctaaaacagc ttcaggcata cagattagat tgcaaagtga
     7081 accggttcag ctatctacat accgtgtttt tgcctatctt agtgaagaaa atatttctcc
     7141 tgcggatgtt gtaactttgc caattgaccc cgataaacat tatagcagtg cttcagaatt
     7201 aatctcgcaa actgcttcct ttcaaggttc cggtattcaa ctgaaaggtg aaatagcgga
     7261 aataaacatt ttgggaaata ttagcagaca cactttggtt ttgctggatg gaattgctat
     7321 gaatacttta ggtgagccct ttgatttttc ccgtttgaat gtggataata tagaaagcat
     7381 agaagttgta aaaaataatg ccagtgttta tggaggtggt tctgccattg gtggaataat
     7441 aatgattact accaaacagg gttccgcgct gaaaaagaac t
//
LOCUS       DAOPFL010000080         7364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_12882_length_7364_cov_108.749897, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000080 DAOPFL010000000
VERSION     DAOPFL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12882_length_7364_cov_108.749897"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLG69_05940"
     CDS             <1..282
                     /locus_tag="PLG69_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43085.1"
                     /translation="QLKNNQLNPDILERQERIISKMLDAQRSINKREFTEKRKAETGE
                     DMLYKGNTKIDLEALRRSGLLEEGLRAYPKEYQQVIMQYLKELNEAINK"
     gene            279..2006
                     /locus_tag="PLG69_05945"
     CDS             279..2006
                     /locus_tag="PLG69_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI43086.1"
                     /translation="MKKAALLILILFTCASAIYAQYDEKQILTQQANQLLMQRQYVQA
                     EQLFLRILDKYPNDTNSINQLMNIYFTLSQTDKAEATLTKYQRTLPPQTYSELLIQLL
                     IMQAKVEEAWQETMRYLNIYGQDEYKYRRLASFFERKAFYDKVIELYTRARNQLGKPD
                     LFRLEMANTAMNYHQFEKAIREYMAYTENNPVYMFYTNNQLKTILQEDATLISVVSAI
                     ADSSNSSVLKEIYANALLNLQDYKTALNIYKQLEPQKMWRFAEEQTAAGNDSIAFEAY
                     SYLETGETDMVKKMDLRYRLAEIKYQQRDYPATEQIIMESLSLPFWKDRNLYYRSSVG
                     VKLRKLMAEIKLAKNEPVDSALVWLNEAKKFSRNNLESQEMDLEIARLYILNGDKIKA
                     QNLLKTINHNTLMEKRDYLNFLNTLLEGETALADTLMNEFIIRYPESQYANDAIYLMM
                     FTLNMAEQDKKSFYSAIRLLQLNRQSGIDTLVVIFSHNQDEEVLLLAIEWAIAFGNDS
                     KAKELLQYDFQDEVAADYAALLRLLMVADKAEEQRLAREFLKNKPNSIFSPDFRQKIS
                     RWSASRPNL"
     gene            2025..3482
                     /gene="guaB"
                     /locus_tag="PLG69_05950"
     CDS             2025..3482
                     /gene="guaB"
                     /locus_tag="PLG69_05950"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPI43087.1"
                     /translation="MNLRKAYTFDDVLLVPQKSDVLPAEVDLSTKITAQISLRIPVIS
                     SAMDTVTESAMAIAMAREGGLGIIHKNLSIEEQAKQVSLVKRAESGIVTHPYILSPED
                     TLDYVLALRDAHHIGGFPVVENEFLVGILTSRDIRFVTNPQTKVKDLMTPKEKLITAK
                     TGISLDKAIDLLQKHRLEKLLLISEEGKLEGMITVKDIMKRLNYPEAVQDNKNRLLVG
                     AAIGVTGDYLERAKELLNAGADLLVIDTAHGHHKNIGIALQKVKKHLNCQVIAGNVAT
                     ADACRYLIENGADAVKVGIGPGSICTTRVIAGIGVPQLSAIMDCALEAGKYNIPIIAD
                     GGIKFSGDIVKALAGGASAVMIGSLFAGCDESPGESIIYNGRRFKSYRGMGSLSAMKQ
                     GSKDRYFQDEQVEDNKLVAEGIEGMVPYKGPVKDFLYQLMGGLRSGMGYCGTATIQEL
                     QEKAEFIEITTAGLKESHPHDVHITKETPNYYSGD"
     gene            3436..4377
                     /locus_tag="PLG69_05955"
     CDS             3436..4377
                     /locus_tag="PLG69_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HPI43088.1"
                     /translation="MSTSLKKPRTITAGIEEELSPALKSYLSSFVYHLKVERGMAKNS
                     IESYRRDIGDFLLFCPKEIGNLETDDITKYLLSLQEIGLLNTSVARKRVALGQFFGFL
                     KDNDIETKVDMDLVPRIKLGVQLPDVLTVEEMLQLLNSLPVKTPLEIRNKLMMELLYA
                     TGMRISELLSISLHDLNLTERVILVHGKGSKQRYVPYVDTLDELFAKYLKQSRPILLK
                     LKQSELLFLNNRGEKMSRMGFWKILRKAVLEANIKKEVTPHTFRHSFATHLLEGGVNL
                     RIVQALLGHSSIDTTQIYTHIDMKRLVETYKEYHPRA"
     gene            4518..5525
                     /locus_tag="PLG69_05960"
     CDS             4518..5525
                     /locus_tag="PLG69_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPI43089.1"
                     /translation="MKNRVNPFNPFCYIIIALTLVLCLSSCKTKSFKQEQAELQKVSV
                     TLDWTPNTNHTGIYVAKELGYFQEQGLEVDILQPGQNVTDQIVATGKSEFGVSYQENV
                     IRARSENIPLVSIAAVIQHNTSGFASLKKAGIKSPLDFEGKRYGSWDSPSELAILRAT
                     MEKYGADFNKVTVISGIYDFFSTIGKDADFEWIYYGWDGVEAERRSIALNYIPLKEID
                     PVFDYYTPVIISSEDYLAKNPETAKKFMTALKKGYEYCIAKPDSAAAILLKNVPELNA
                     EQVKRSMQYLAKEYQSDAESWGIQKSAVWKRFCEWMYQQRLIQLAVDYNKAFTNDYLP
                     Q"
     gene            5531..6337
                     /locus_tag="PLG69_05965"
     CDS             5531..6337
                     /locus_tag="PLG69_05965"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPI43090.1"
                     /translation="MPNCILEAKDIRKTFLFKGALVVVLNKLQFSIREGQFVSIVGPS
                     GCGKSTFLELLAGITKPDNGNIYLEGNEITGKTGFLGYMPQDDLLFPWLTTIQNILLP
                     VKVQGKDEKEARKKIIELLPLFGLMNYTDHLPYQLSGGLKQRVALLRTYMSSAEILLL
                     DEPLAKLDALTRSQLQTWLKDIVHLLNLTVILVTHDIDEAIMLSDRIDLMSINPGTFM
                     ESIYLDRNAPLSAEEQLKLKTKIRSKLVNGEEKSENGKKNGEREKSDKVI"
BASE COUNT         2468 a         1253 c         1577 g         2066 t
ORIGIN      
        1 caactgaaaa ataatcagct caatcccgat atcctggaaa gacaggaaag aattatcagc
       61 aaaatgctgg atgctcaacg ctctatcaat aaaagggaat ttacggaaaa acgcaaagct
      121 gaaacgggtg aagatatgct ctataaagga aatacgaaaa ttgatttgga agctttaaga
      181 cgcagcggac ttttagaaga aggtttgcgt gcttatccta aagaatatca acaggttata
      241 atgcaatatc tgaaagagct gaatgaggca ataaataaat gaaaaaggca gctctcctaa
      301 tactgatact atttacttgc gcaagtgcaa tatatgcaca gtatgatgaa aagcaaattc
      361 ttacccagca ggcaaatcaa ttgctgatgc aacgccaata tgttcaggcg gaacagctct
      421 ttttacgcat cctggataaa taccctaacg acacaaattc catcaatcag ctgatgaata
      481 tatattttac tctgtcccaa acagataagg cagaagcaac cttaaccaaa taccaaagaa
      541 ctttgcctcc gcaaacttat agcgaactgc ttattcagct gctaattatg caagcaaaag
      601 tagaagaggc ctggcaggag acaatgcgtt atttaaacat ctatggccag gatgaatata
      661 aatatagacg gttagcttct ttctttgaac gcaaggcatt ttacgacaaa gtgatagagc
      721 tttatacaag agcaagaaat caattgggaa aaccggattt atttcgtttg gaaatggcta
      781 atacggcaat gaattatcat caatttgaaa aggcaattcg tgagtatatg gcttatacag
      841 aaaataatcc ggtctatatg ttttatacca ataatcagct gaaaacaata ttacaagagg
      901 atgcaaccct tatttccgtt gtttctgcta ttgcggacag cagtaatagt tctgtgctga
      961 aagaaattta tgccaatgcc ttgctcaatt tgcaggatta taaaacagct ctgaatattt
     1021 ataaacagct ggaaccccaa aaaatgtggc gttttgccga agaacaaaca gctgcaggta
     1081 acgatagcat tgcttttgag gcatacagct acctggaaac aggagaaacg gatatggtta
     1141 aaaagatgga tttgcgttac cgcttggctg aaataaaata tcaacaaagg gactatccag
     1201 ctactgaaca aataattatg gaatcgctat ctctgccttt ctggaaggat agaaatctct
     1261 attatcgcag ttctgtggga gttaaattac gcaaattgat ggcagaaata aaattggcta
     1321 aaaatgaacc tgttgatagc gctttggttt ggttgaatga agccaagaaa tttagtcgta
     1381 acaacttgga atcgcaggaa atggatttgg aaattgcccg cctgtatata ttgaatggag
     1441 ataaaattaa agcccaaaac ctcttgaaaa ccatcaatca taataccctg atggaaaaaa
     1501 gggattacct gaacttcttg aatactctgc tggaaggtga aactgcctta gcggatactt
     1561 tgatgaacga gtttatcatt cgctatcccg aaagccaata tgccaacgat gctatctacc
     1621 tgatgatgtt tactttgaat atggcagagc aggacaaaaa gagtttctat tccgctattc
     1681 gtttattaca actgaaccgt caaagcggaa ttgatacttt ggtagttatc ttcagtcata
     1741 atcaagatga ggaagttctt ttactggcaa ttgaatgggc tattgctttc ggcaatgact
     1801 ccaaagcaaa agagttactg caatatgatt ttcaggatga agttgctgcg gattatgccg
     1861 ctttattacg actgttaatg gttgcggata aagcggaaga acaacgactg gcaagagaat
     1921 ttttaaaaaa caaacccaac agtatttttt cacctgattt taggcaaaaa ataagtcgtt
     1981 ggtcagcttc tcgaccaaat ttatagttct taacaggaga taaaatgaac ttacgcaagg
     2041 catacacctt tgatgatgtt ctgctggtgc ctcaaaaatc ggatgtctta cctgcagaag
     2101 tggatttgag cacaaaaata acggctcaaa tatctttacg gattcctgta atcagttccg
     2161 ctatggatac tgtaacggaa tccgctatgg caattgctat ggcaagagaa ggaggattgg
     2221 gaattattca taaaaacctt tctattgaag aacaggcaaa gcaggtcagt ttagtaaaaa
     2281 gagcagaaag cggaattgtg actcatccct atattctttc gccggaagat actttggatt
     2341 atgttttggc tttgagagat gcacatcata tcggtgggtt tccggttgta gaaaatgaat
     2401 tcctggttgg tattttaacc agtagagata tccgttttgt aaccaatccc caaacaaaag
     2461 tgaaagacct gatgactcct aaggaaaaac tgattactgc taaaaccgga atttcactgg
     2521 ataaggcaat tgacctttta cagaaacatc gcttggaaaa actcctgctg attagtgagg
     2581 aaggcaaact ggaaggaatg ataaccgtta aagatattat gaaacgctta aattatcccg
     2641 aagcggttca agataataaa aaccgtttac ttgtaggtgc tgcaattgga gttacgggtg
     2701 attaccttga aagagcaaaa gaacttctta atgccggagc cgatttactg gtaatagata
     2761 cggcacatgg tcatcataaa aacattggca ttgccttgca aaaagtaaaa aagcatttga
     2821 actgtcaagt tattgcaggc aatgtagcta ctgcagatgc ctgccgctat ttaattgaga
     2881 acggagcaga tgcagttaaa gttggcatcg gtcctggttc catttgcaca accagagtta
     2941 ttgccggaat tggagttccg caattaagcg ccataatgga ttgtgctttg gaagcaggta
     3001 aatacaacat tcctattata gctgatggag gcataaaatt cagcggggat attgttaaag
     3061 cacttgctgg aggtgcttct gcagttatga ttggttcact ttttgccggt tgcgatgaaa
     3121 gccccggaga atccataatt tacaatggca gaaggtttaa aagttaccgg ggaatgggtt
     3181 ccctatccgc tatgaagcaa ggcagtaaag accgctattt tcaagatgaa caagtagaag
     3241 acaacaaact tgtagccgaa ggaattgaag gaatggttcc ctataaaggt cccgttaagg
     3301 attttctcta tcaattgatg ggtggattgc gttccggaat gggttattgt ggaactgcca
     3361 ctattcagga attacaagaa aaagccgaat ttattgaaat cacaactgcc ggcttgaagg
     3421 aaagtcatcc tcacgatgtc cacatcacta aagaaacccc gaactattac agcggggatt
     3481 gaagaagagc tttctcctgc tttaaaaagt tacctttcca gttttgttta tcatttgaag
     3541 gtagagcggg gaatggcgaa aaacagtatt gagagttatc gtcgtgatat aggtgatttt
     3601 ctgctatttt gccctaagga aattggtaat ttggaaacgg acgatattac caagtatctt
     3661 ctttctttgc aggaaattgg acttttaaat accagcgttg ccagaaagag agttgccttg
     3721 ggtcaattct tcggtttttt aaaggataac gatattgaaa ctaaggtaga tatggatttg
     3781 gttccgcgta tcaaactggg agtgcaatta ccggatgttt taactgtgga ggaaatgttg
     3841 cagctgttaa attccctgcc tgttaaaaca cctttggaaa ttcgcaataa gttaatgatg
     3901 gaattgctgt atgcaacagg tatgcgaatt tccgaactct taagtatttc tttgcacgat
     3961 ttgaatttaa cggagcgggt tattttagtt cacggcaagg gcagtaagca aagatatgtg
     4021 ccttatgtag atacgcttga tgaactgttt gcaaaatact taaagcagag tcgtccaata
     4081 ctattaaagc tgaaacaatc ggaattgctt tttctgaata atcgcgggga aaaaatgagt
     4141 cggatgggtt tctggaaaat attacgcaaa gcggttttgg aggcaaatat caaaaaggaa
     4201 gtaacgccac atacttttcg gcatagtttt gccactcatt tattggaagg aggagtgaat
     4261 ttaagaattg tgcaagccct tttgggacat tcttccattg atacaacgca gatttataca
     4321 catattgata tgaagcgact ggtagaaaca tataaagagt atcatccacg cgcgtaaaaa
     4381 aggtgaacag cgaatggtga acagtgaaaa aaaagaattg aaaattgaga attacaaata
     4441 ataatctgcg aaatctgcaa aatctgcgtg aaataaaatt tttatctgtg aaatctgtaa
     4501 gaaaaaaagg agcataaatg aaaaaccggg ttaatccgtt caatccattt tgctatataa
     4561 ttatagcgct tacattagtt ctttgtttat catcctgtaa aacgaagagt ttcaaacagg
     4621 aacaggcaga attacaaaag gtttcggtta ctttagattg gactcccaat accaatcata
     4681 ccggaatcta tgtagctaaa gaacttggct atttccagga acagggcttg gaggtggata
     4741 ttttgcagcc gggacaaaat gttactgacc aaattgttgc cactggaaaa agtgaatttg
     4801 gcgtaagtta tcaggaaaat gtaattcgtg ccagaagtga aaatattcct ctggtttcaa
     4861 ttgcagccgt tattcagcat aacacttccg gttttgcctc tttgaaaaaa gcgggaataa
     4921 aaagtcctct tgattttgaa ggtaaaagat acggttcctg ggattcgcct tcagaacttg
     4981 ctatcctgag agcaactatg gaaaaatatg gtgccgactt caataaagta accgttattt
     5041 ccggaattta cgatttcttt tccactattg gtaaagatgc agattttgag tggatttatt
     5101 atggctggga tggagtggaa gcggaaagaa gaagcatagc cctaaactat atacccctca
     5161 aagaaataga ccctgttttt gactattata cacctgtaat tatcagcagc gaagattatc
     5221 tggcaaaaaa tcccgagacc gccaagaagt ttatgacagc ccttaaaaaa ggttatgaat
     5281 attgtattgc caaacccgat tccgctgccg ctattttgct gaaaaatgtc cccgaactta
     5341 atgccgaaca ggttaaacgc agtatgcaat atttggcaaa agaataccaa agcgatgctg
     5401 aatcttgggg aatacaaaag tcggctgtct ggaaaaggtt ttgtgaatgg atgtatcagc
     5461 aacgccttat tcagcttgca gttgactata ataaggcatt tacgaacgac tatctacctc
     5521 aataaattct atgcctaatt gcattctgga agcgaaggac attcgtaaaa catttctctt
     5581 caagggagcg ctggttgttg ttttaaataa attacaattc agcatccgag aaggtcagtt
     5641 tgtcagtatt gtaggtccaa gtggttgtgg taaaagcact ttcctggaac ttttggctgg
     5701 aatcactaaa ccagataatg gcaatattta tcttgaaggc aatgaaatta caggaaaaac
     5761 gggttttttg ggttatatgc cgcaggatga tttgcttttt ccctggctaa ccacaataca
     5821 aaatattctc ctgcccgtaa aagtgcaagg caaagacgaa aaagaggcaa gaaagaaaat
     5881 tattgaactt ctccccctgt tcggtttaat gaattatacg gatcatttac cctaccagct
     5941 ttcgggggga ttaaaacaac gtgttgcgct tttaagaacc tatatgagca gtgcggaaat
     6001 cttacttttg gacgaacccc tggcaaaatt ggatgcttta acgcgcagtc aactgcaaac
     6061 ctggctgaaa gatattgtcc atttactaaa tctaaccgtt attttagtta cccatgatat
     6121 tgacgaagca attatgcttt cggatagaat tgacctgatg agtatcaatc ccggaacttt
     6181 tatggaaagc atatatctgg atagaaatgc acccttaagc gctgaagaac agctaaaact
     6241 gaaaacaaaa atacgcagta aactggttaa cggagaagaa aaaagtgaaa atggcaagaa
     6301 aaatggtgaa cgggagaaaa gtgataaagt gatatagatt acaagtgttt agcgggagga
     6361 cgctgttact ccgctaagaa aaaaaagcgg agcttaggca agctctggct acacaaatcg
     6421 tgtaataaga aaatactcgc aaatcaataa aatccgtgca atctgtgtaa tctgtgagag
     6481 ccgtcctttc ttgttagcca tcatcaactc aacctaaaag atggatgaag gatgggttgt
     6541 aattaggcaa gcttatatgg ctgtgttgtt tttggttaaa ttatagcaga gtgcaaagag
     6601 cgaagggaat aaatattatt actatgataa aaagttccgt aggaacgaca gatattaaca
     6661 acgggtttta acccggtgaa aagagaaata tgaaacaact tatttctttt gttaagaaag
     6721 agttccgtag gaacgacaga tattaacaac gggttttaac ccggtgaaaa gagaaatatg
     6781 aaacaactta tctcttttgt taagaatgag ttccgtagga acgacaggta ttaacaacgg
     6841 gttttaacca ggtgctatga agatagacga tatcttattt cattttttcc aaatgagttc
     6901 catagtaaag tggaagttgc caaagcattt gttctcaact gcattttggg ggttaaataa
     6961 agtccgctgt tttcaaggta tttcagcttt tttcagcttg aattgctgaa gcattgctta
     7021 cccattacgg atgggattag caatgggtaa gcaatgagta agcaatgcta ttaaggaaga
     7081 atatgtaatt atatttagat aataggagtt aggaaaattt aggggaagaa gataatatta
     7141 tatctgtaac tccatttttt atatagtttt aatattcata aaccctatat agtaagataa
     7201 atcttatagg gatggcagtt agataacgac gagttttaac ccggtgcaag aggagaaaaa
     7261 ccacaaaact atccttattt tctggaaaaa gttccgtagg aacgacaggt attaacaacg
     7321 ggttttaacc cggtgaagaa gaacatcaga gagccattat ctct
//
LOCUS       DAOPFL010000081         7362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_12889_length_7362_cov_108.820036, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000081 DAOPFL010000000
VERSION     DAOPFL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12889_length_7362_cov_108.820036"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3144
                     /gene="secA"
                     /locus_tag="PLG69_05970"
     CDS             <1..3144
                     /gene="secA"
                     /locus_tag="PLG69_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPI43091.1"
                     /translation="KKVFGDKNSQDLKRYEPVVQEINEIFSGLEQYEDEQLIARVQEI
                     KQEIADKLTPLRNELAKLEQEYRETREDSERNRLDNAIDSTKKELKNLTKSTLDDYLP
                     EVYAIVKDTCRRLLGKKFEVRGHEVEWNMVPFDVQLIGAMALHDGKIAEMATGEGKTL
                     VATMPLFLNALVGRGVHLVTVNDYLASRDAEWMSPVFQFHGLTVGCITTGMDFDARKE
                     AYNCDITYGMNSEFGFDYLRDNMATSPDQLVQRDFFYAIVDEVDSILIDEARTPLIIS
                     GPIAQDKNFYHELRPAIAQLVQLQNSLVQKYLSEIREDLDENNPQSDNNRLAKNLLLV
                     KRAAPRNKAFMKLMQDGELKKMVNDIEGIYLRDKKLPELDNNLFYVVEERHNSVDLCE
                     KGREVLSRKDKDLFVVQSLDEMLAEIDNNPELSEQEKQKEKSLQTNRFMDKSEKLHNI
                     NQLLRAFTLFENDQEYVVIDNKVVIVDEFTGRQMPGRRFSDGLHQALEAKENVEIEAG
                     TQTFATITLQNYFRMYEKLAGMTGTAVTEEAEFMEIYNLPVMVIPTNVPVTRIDHDDL
                     IYLGKNDKYQAIINEIIYWHERQKPVLVGTVSVEVSEIISRLLKRKGIAHNVLNARQH
                     QREAEIIAEAGQPGAVTIATNMAGRGTDIKLGKGVVEGTYESYLNLPKTLTAEFPYGL
                     PLDGLHVIGSERHESRRIDRQLRGRAGRQGDPGTSRFYLSLEDDLMRLFGSDRIAPMM
                     VKMGLKSGDVIRHPWMTKAVEQAQKRVEEHNFEIRRELLKYDEVMNQQREVIYAYRRS
                     VLKGYDLKNEILEMITESITNMVDDIIPPHSYPEDWNLERICQWFQHNLNLGLTVKDI
                     TSDHLNRDLLLNTLLEYALSAYENKEKQIGSEQLRNIERRALLEVVDDEWRDHLHEMD
                     LLKDGVYLRAYANKDPLIEYKKESFELFQGLITRIQENVTRKVFTTYVLSQEQINNLL
                     KNANLTHQDINAFLNAQQAQQMTQNMSAPPQYNNPVGEVEKVRPIKVAPKVGRNDPCP
                     CGSGKKYKKCCGINEAE"
     gene            3190..5409
                     /gene="topA"
                     /locus_tag="PLG69_05975"
     CDS             3190..5409
                     /gene="topA"
                     /locus_tag="PLG69_05975"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPI43092.1"
                     /translation="MSKGLIIVESPAKAGTISKFLKNQFSVKASMGHIRDLPKHELGV
                     NVHQGFKPVYIIDKTKSKVIAELKEATLSADSVYLASDNDREGEAIAWHLSETLKKEL
                     NNKPVYRIVFNEITSQAINEAIKNPGQIDMAKVEAQQARRILDRLVGYEISPLLWKVI
                     TKDLSAGRVQSVALRLICEREAEIKAFVPKEYWKIEADFWKNNLPPFKAVLEKIEGKK
                     VEIPDEKSAIEIVENIQNAEAILSEIKRSNRNVEPLPPFITSTLQQEASKILGFQAQK
                     TMSIAQVLYEGIDLGGERTGLITYMRTDSTRMAEEAITKAQNLIKERFGKELVNPKVR
                     VFKNKQSAQDAHEAIRPTDPSRTPESIAQYLTKEQMKLYTLIWQRFIATQMIPVKLLS
                     TSVKINAGKAEFVSSGAEIMEEGFLKAYPHYNIPLGEKIHQDYTKNDVLEHSPLEKTQ
                     HFTTPPARYTEASLIKELEAKGIGRPSTYATIIETLRKRKYVTMEKKAFLPTQLGSDV
                     NRFLVDKFDYLFNVQFTAEMETKLDEVEYSKIAWNEVVQEYYDQLVTLIRQVDVKKEK
                     NNFVQDTGIVCDVCKQGTMLIKHSKRGDFLSCSRFPQCKNTKNFTHDEDGNLKIFVPT
                     PLNENCPQCGSPLMLRNGKYGEFIACSNYPKCKYARPKTLGIKCPECGIGELTARKSK
                     KGRSFYSCNRYPECKYITNDKPLPISCPKCGNPYIVEKYSREKGKIKICPNCKTEME"
     gene            5413..6684
                     /locus_tag="PLG69_05980"
     CDS             5413..6684
                     /locus_tag="PLG69_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI43093.1"
                     /translation="MNIKDGINSAFQSIFSHKLRSLLTLTGIVIGVLAVVTMFSSVYA
                     IKALIKKNMEGMGWDNSIIIFPGSGNIDLETGKTRSKIRRAKQNVPPLNYDDYLALKE
                     NLNYKCIYGTISQHSLYRVGNKINNIILRATEVEFFQNKSYPISKGRYFNAYEAENNI
                     PVAVLGYHFAEEYFKDKDPIGQVLVLGSQRFTIVGVLASDVLNTGNGMNFDPWQREWD
                     LKAVYIPLKYGATYLSPARMIHQIYIQSNSSEDYAKVKNEARQILLARHNMYPNFQFM
                     DIGDMILNITQEINKFMDKWNVTLIAIASISLIVGGIGLFSTLLISIQERMTEIGIRK
                     SIGATEQDIFFYFIFEALALAFIGAILGVVLAWILIVLIAKGINFPLYLPVQGVAVGI
                     GFSLLVGFLSGIYPAWKATGIDPIQAIYYHE"
     gene            6774..7355
                     /locus_tag="PLG69_05985"
     CDS             6774..7355
                     /locus_tag="PLG69_05985"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPI43094.1"
                     /translation="MKKTDKIAEFLAKKRLLITRILCIAVALEFIINYKDRIQHLLTN
                     PSGIIISIILIALGAFIRSWSAGIIQKGNRLVQEGPYCLNRNPLYTGSALILIGFVLF
                     LNDIWAWIVAILLILVIFPFTISREEKGLRNKFPEEWINYSKTTGRYFPRKISWKKMK
                     YPWSFQLWLKNREYQTFFLSLLLLIIIIIVGLV"
BASE COUNT         2454 a         1323 c         1567 g         2018 t
ORIGIN      
        1 aagaaagtat ttggcgataa gaacagtcaa gaccttaagc gctatgaacc cgtagtgcaa
       61 gaaataaatg aaatattttc cggattggaa caatatgaag atgaacagct aatcgctcgc
      121 gttcaggaaa ttaaacagga aattgcagat aaactaactc ctttgagaaa tgaacttgct
      181 aaactggaac aggaatatcg ggaaaccaga gaagatagcg aaagaaatcg tttagacaat
      241 gcaatagaca gcactaaaaa agaactgaaa aatcttacca aaagcactct ggatgattat
      301 ctgcccgagg tttatgccat tgttaaagat acctgccggc gtttgttagg caaaaaattt
      361 gaagtccgcg gtcatgaagt tgaatggaat atggttcctt ttgatgtgca attaattgga
      421 gctatggctt tacacgatgg taaaattgcc gaaatggcta ccggagaagg aaaaaccctg
      481 gttgctacta tgcctctgtt tttgaatgcc ttagtgggac gtggcgtgca tcttgtaacc
      541 gtaaatgatt atctggcaag tcgtgatgct gaatggatga gtcctgtttt tcaatttcac
      601 ggactcactg taggttgtat tactaccgga atggattttg atgctcgcaa agaagcatac
      661 aattgtgata taacttacgg aatgaacagt gaatttgggt ttgactattt gcgtgataat
      721 atggcaactt ctcccgatca gcttgtccag cgggatttct tttatgcgat tgtggatgaa
      781 gtagatagca ttttaattga tgaggcaaga acccctctaa taattagtgg tcccattgcc
      841 caggataaga atttctatca cgaattgcgt cctgcaatcg cgcagcttgt tcagttacag
      901 aattctttgg tacaaaaata cttaagtgaa atccgagagg atttggatga aaataatccc
      961 cagtcagata ataaccgctt agctaaaaac ttgcttttgg tgaaaagagc cgctccgcgt
     1021 aacaaagcat ttatgaaatt gatgcaggac ggtgaactga aaaaaatggt caacgatatt
     1081 gaaggtatct atttgcggga taaaaaatta cctgaactgg acaacaacct tttctatgtg
     1141 gtggaagagc gtcataatag cgttgacctc tgtgaaaaag gaagagaagt gctttcccga
     1201 aaggataaag accttttcgt tgtccaatcc ctggatgaaa tgctggcaga aatagataac
     1261 aatcccgagc tctcggaaca ggaaaaacag aaagaaaagt cattgcagac caatcgtttt
     1321 atggataaaa gcgaaaagct gcataacatt aatcagttac tgcgcgcctt taccctcttt
     1381 gaaaacgatc aggaatatgt ggtaatagat aacaaagtag ttattgtaga tgaatttaca
     1441 ggacgtcaaa tgccgggacg ccgtttttct gatggtttac atcaggcatt ggaggcaaaa
     1501 gaaaatgtgg aaattgaggc aggcacacaa acatttgcca ctattacttt gcagaactat
     1561 ttcagaatgt atgaaaaact tgccggaatg acaggaaccg cagttaccga agaagccgaa
     1621 tttatggaga tttacaattt gccggtgatg gtgattccga caaatgttcc ggttacacga
     1681 attgaccacg atgatcttat atatttaggc aaaaatgata aataccaggc aatcatcaat
     1741 gaaataattt actggcatga aaggcaaaaa ccggttttgg taggaactgt tagtgtggag
     1801 gtctcggaaa ttatttcccg tttactaaag cgcaaaggta tagctcataa tgttctaaat
     1861 gcccgtcaac atcaacgcga agctgaaatt atcgctgaag cgggacaacc tggagctgta
     1921 actattgcta ccaatatggc aggtagagga accgatataa aacttggcaa aggtgttgtg
     1981 gaaggtactt atgaaagcta tctgaatttg cctaaaacat taaccgcaga atttccttac
     2041 ggcttacctt tggacggact ccatgtaatt ggcagtgaaa gacatgaaag tagaagaatt
     2101 gatagacaat tacggggaag agcaggccgc cagggtgatc ctggaacctc aaggttctat
     2161 ttatctttgg aagacgattt aatgcgcctt ttcggttcgg acagaatagc tccgatgatg
     2221 gttaaaatgg gcttaaaatc gggagatgta attcgtcacc cctggatgac caaagctgtg
     2281 gaacaagcac aaaaaagagt ggaagagcac aactttgaaa tccgcagaga actgctgaaa
     2341 tatgacgaag taatgaatca acaacgcgaa gtgatttatg cctaccgtcg cagtgtgctt
     2401 aagggttatg accttaaaaa tgaaattctg gaaatgatta ctgaatccat taccaatatg
     2461 gtagatgata ttattccacc ccatagctat cctgaggact ggaatctgga acgcatctgc
     2521 caatggtttc agcataatct gaatttggga cttacggtta aagatataac cagcgaccat
     2581 ctgaatagag accttctgct gaatacgctt ttggaatatg cactttccgc ttacgaaaac
     2641 aaagaaaaac aaatcggcag cgaacaatta cgcaatatag aaagacgagc cctcttagaa
     2701 gttgtagatg atgaatggcg tgaccacctg catgaaatgg acttattaaa agacggtgtc
     2761 tatttacgcg cttatgccaa taaagaccct ttaatagaat ataaaaagga aagctttgaa
     2821 ctgtttcaag gtttaattac ccgcattcag gaaaatgtta cccgcaaagt gtttactact
     2881 tatgttctat ctcaagaaca aattaacaac ctgctcaaaa atgcaaacct gactcatcag
     2941 gatataaatg ctttcctgaa tgcacaacag gcacaacaaa tgactcaaaa tatgagcgct
     3001 cctcctcaat ataataatcc tgtgggagaa gtagaaaaag tccgacccat aaaagtagct
     3061 cccaaagtgg gaagaaatga tccctgccct tgcggaagcg ggaaaaagta taaaaaatgc
     3121 tgcggaataa atgaagcaga atagaagttt agaggcctgg aggtcaagag gaaagtgaaa
     3181 aggtaatata tgagtaaagg acttataata gtagaatccc ctgcgaaagc aggaactatc
     3241 agtaaatttc tgaagaacca attcagtgtg aaagcatcaa tgggtcacat tcgtgatttg
     3301 cctaaacacg aactgggagt taatgtccat cagggtttca agcccgttta tataatagat
     3361 aaaacgaaaa gcaaagtgat tgcggaactt aaagaagcaa ctttatccgc ggatagcgtt
     3421 tatctggcaa gtgataatga tcgtgaagga gaagcaattg cctggcactt atccgaaacc
     3481 ctgaaaaagg aattaaataa taaacccgtc tatcggattg tgtttaatga aataacttcc
     3541 caagcaatca atgaggcaat caaaaacccc ggtcaaattg atatggcaaa agtggaagcg
     3601 caacaagcaa gaagaatatt agaccgcctg gttggttatg aaatcagtcc tttgctgtgg
     3661 aaagtgatta ccaaagacct ttctgcggga agagtgcaat cggttgcttt gcgtttgatt
     3721 tgtgaacgcg aggcagaaat aaaagcattt gtccctaaag aatactggaa aattgaagcg
     3781 gacttttgga aaaacaattt gcctcccttt aaagctgtat tggaaaaaat agaaggcaaa
     3841 aaagtggaaa ttccggatga gaaaagtgcc atagaaatcg tggaaaacat tcaaaatgca
     3901 gaggcaattt taagcgaaat aaaacgcagc aatcgtaatg tagaacccct tccacctttt
     3961 attaccagta ctttgcaaca ggaagcgagt aaaatacttg gttttcaagc acaaaaaaca
     4021 atgagcattg ctcaagttct ttatgaaggt attgatttgg gtggagaaag aaccggtctg
     4081 ataacttata tgcgaaccga ttctaccaga atggcggaag aggcaattac taaagctcaa
     4141 aaccttataa aagaacgctt tggcaaagaa ctggtgaatc cgaaagtccg agttttcaaa
     4201 aataaacaga gtgctcagga tgcgcatgaa gcaattcgtc ctactgatcc ttccagaact
     4261 ccggaaagca tagcccagta tttaaccaaa gaacagatga aactttatac ccttatctgg
     4321 cagcgtttta tagctacgca aatgataccg gtaaaactcc tttccacaag tgtaaagata
     4381 aatgctggaa aagcagaatt tgtgtcttcc ggagctgaaa taatggaaga gggtttcctg
     4441 aaagcatatc ctcactataa tattcctttg ggtgaaaaga ttcatcagga ttatactaaa
     4501 aatgatgttc tggaacatag tcctttggaa aagacacagc attttactac tcctccggca
     4561 agatacactg aagcatcttt aatcaaagaa ctggaggcaa aagggattgg gcgtccttct
     4621 acttatgcta ccattataga aacattaaga aagcgtaaat atgttactat ggagaaaaag
     4681 gcatttttgc cgacgcaatt gggaagtgat gtaaaccgct ttttggtgga taagtttgat
     4741 tatcttttta atgttcagtt tactgccgaa atggaaacca aactggatga agtggaatac
     4801 agtaaaattg cctggaatga agtggtgcag gaatattacg accaacttgt taccttaatt
     4861 cgtcaggtag atgtgaagaa ggagaaaaac aattttgttc aggataccgg cattgtttgt
     4921 gatgtttgca aacagggaac aatgttaatc aaacacagca agaggggcga ttttctttcc
     4981 tgcagccgtt ttccccaatg caaaaacacg aagaatttta cccacgatga agatggcaac
     5041 ctcaagattt ttgtgcctac acccttaaat gagaattgtc cgcaatgtgg ttctccttta
     5101 atgttaagaa acggaaaata tggagagttt attgcctgtt ccaattatcc caaatgcaaa
     5161 tatgcccgtc cgaaaacctt aggaattaaa tgtccggaat gcggaatcgg tgaattaaca
     5221 gcccgtaaat ccaaaaaagg acgcagcttt tattcctgca atcgctatcc ggaatgcaaa
     5281 tatataacta acgataagcc tcttccgata agctgtccca aatgtggaaa tccttacata
     5341 gtagaaaaat acagccggga aaaaggaaaa attaaaatct gccccaactg caaaaccgag
     5401 atggaatagg caatgaacat taaagacggc atcaatagcg ctttccaaag cattttcagc
     5461 cataaactga ggtctttgtt aaccctaaca ggaattgtta ttggagtgct tgccgtggta
     5521 acaatgtttt ccagtgttta tgccataaaa gcacttatca aaaagaatat ggaaggtatg
     5581 ggctgggata attctattat tattttcccc ggttcaggaa atatagattt ggaaacagga
     5641 aaaacgcgta gtaaaattag gcgtgctaaa caaaatgttc ctcctttgaa ctatgacgat
     5701 tatctggctc tgaaagaaaa cctgaattac aaatgtattt acgggacaat ctcccaacat
     5761 agtttatatc gggttggcaa taaaataaat aatattatct tgcgtgctac agaagtagaa
     5821 tttttccaaa acaaaagcta tcccatcagc aagggacgct attttaatgc ttacgaagcg
     5881 gaaaataata tcccggttgc cgttttgggt tatcattttg ccgaagaata ctttaaggat
     5941 aaagacccga ttggtcaggt tctggtttta ggttcacagc gttttaccat cgtaggtgtt
     6001 ttagcttcag atgttttaaa taccggaaat ggaatgaatt ttgatccctg gcaaagagaa
     6061 tgggacttga aggctgtgta tattccctta aaatatggag ctacttattt aagcccggca
     6121 agaatgatcc atcagattta tatacaatca aattccagtg aggactatgc caaagtaaaa
     6181 aacgaggcaa ggcaaattct gcttgccagg cataatatgt atcccaattt tcagtttatg
     6241 gatattggcg atatgatctt aaatattacg caagagatta ataaatttat ggataaatgg
     6301 aatgtaaccc ttattgccat tgcttccatt tctttgattg tagggggaat aggacttttc
     6361 agcactctgc tgattagcat tcaggaaaga atgaccgaaa ttggtatccg caaaagtatt
     6421 ggtgcaaccg aacaggatat ctttttctat tttatttttg aagcgctggc tttagctttt
     6481 attggagcaa ttttaggtgt agttcttgcc tggattttga ttgttttgat agctaaaggc
     6541 attaatttcc ccttgtatct tccagtgcag ggagttgctg ttggaattgg tttttctcta
     6601 ttagttggct ttctttccgg aatctatcct gcctggaaag ctaccggaat tgatcccatt
     6661 caggcaattt actaccacga atgaaaacac cggaagaacg atttcctcgt tattgctaac
     6721 tttgcttcta taaataatgc gttaaaaaat aacaaagtaa gggagtagct ccagtgaaaa
     6781 aaaccgataa aatagccgag tttctggcaa aaaaacgctt actcatcaca cgtatcttgt
     6841 gcattgctgt tgctttggaa ttcatcatca attataagga tcggattcag catctgctaa
     6901 ctaatccctc aggtattatc atcagcatta ttctaattgc tttaggtgct tttatcaggt
     6961 cctggtctgc cggaattatt caaaaaggta atcgcctggt gcaagaagga ccttattgct
     7021 taaatcgcaa tccgttatac acaggcagcg ctttaatttt aatcggattt gttttattcc
     7081 tgaatgatat ttgggcttgg atagtagcaa ttctcttgat tctggtaatt ttccctttca
     7141 ccattagcag agaagaaaaa ggtctgcgca ataaatttcc tgaggagtgg attaactatt
     7201 ccaaaacaac cggaaggtat ttcccccgga aaatttcctg gaaaaaaatg aaatatccct
     7261 ggtctttcca gttatggctt aaaaacaggg aataccaaac ctttttccta tctctccttc
     7321 tcctgataat aattatcata gttgggttgg tttagagggt tg
//
LOCUS       DAOPFL010000082         7279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_13070_length_7279_cov_139.618909, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000082 DAOPFL010000000
VERSION     DAOPFL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13070_length_7279_cov_139.618909"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PLG69_05990"
     CDS             <1..1584
                     /locus_tag="PLG69_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPI43095.1"
                     /translation="KAEKKGLLRNQKILAWLDFRFACGRLCNTIKAKTPIMLSRPCLN
                     LKSARHPLLILRLGNYRKVIPFDLELGEEKKIIILSGPNTGGKTVLLKAVGLITLMAL
                     SGLPVPADADSEIGFFSNVFADIGDDQSIENALSTFSAHLEKIGKMLNKAKPDTLVLV
                     DEIGAATDPQQGSALAQAILEKFLSLGCPAIVTTHYTALKIFAEQHPRCLNASMQFDT
                     KNLQPTYKFIAGFPGDSFAIEVAASLGIDPTLIERAKELAGNQNVQFTELLKKMQDEK
                     KKYSVSCYQNELKTHLLETKLQELAKKEEEWEKEVKIRRQNHLKELQQELIAQQKIYQ
                     NDLAELKTLSKQERKTFSEYKLHNIAVKNAEIQKELDKDLSANNLPVSNPQPGMKVWL
                     SNFETEAVILSIEGEQVKVDMNGITFKTPVSTLFEPKTKTETTKIEPKVKANVAPVAK
                     FELKIIGLTFEEAKPLIDEFLDNAALAGLHSLRIVHGKGTGALRTKVRDYLKKRKEVK
                     GVGTPSPNEGGTGVTIVTI"
     gene            1648..3369
                     /gene="dnaG"
                     /locus_tag="PLG69_05995"
     CDS             1648..3369
                     /gene="dnaG"
                     /locus_tag="PLG69_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPI43096.1"
                     /translation="MDQNLIDQIRRANDIVDVIQGYLPLKRVGSNFRGLCPFHNDSRP
                     SLYVSQSKQIFKCFACGKAGNVIGFVADFEKLTFIEAVKKLAQRAGIQVPEYEKTKVV
                     NTKREQLLSVYKSAAEFFTSSLFAFGQNVLDYLKKRSFSPETAKELQLGYALNSEKAL
                     LNHLLKEGYSVSLLKDSGLFGNYGGGLTDLFKDRLMFPIHNSLGEVIAFGGRILEPKE
                     GVGKYINSPGTELYTKGKELYGLFKTKYNISKAGTAIICEGYFDFLRLYENGFTNSVA
                     SLGTALTEDQINLLARFCNRVIMLYDGDSAGIKAAVRAGLLCLSRGMEVLIANLPAEE
                     DPDSLILKQGKMAMQEVVDKSIPLINFLATDPRPEQPTAERIELILDALRILKDRIKQ
                     ELLLQEVSDAFGITVGALNSKLRRSSASVSAESSEQTVPQYESFEERNVLALALKDYE
                     SYKLLANDLDSSYFNNKRYRQVYNFLITQNLKSEPWEPAALLDNIENNEIKESLAELL
                     FEDLQQMCFEDCLTGLRIRKVQRDLEEMDRAISKDPKNMELLKEKEKLALKYRQMTKK
                     VVNKVLY"
     gene            3392..5131
                     /gene="rpoD"
                     /locus_tag="PLG69_06000"
     CDS             3392..5131
                     /gene="rpoD"
                     /locus_tag="PLG69_06000"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPI43097.1"
                     /translation="MITYNECLKKLVELGKESGYITFKQINEILPPSPFFLDKVDDII
                     FDLSQDGIEIIDETEKRITKSGAAIRKPSTSKKFKSKRYYDDPVRMYLREMGRVPLLD
                     REGEVRVAKKIETYQKMINQNVFKCGSTLREMYNFLHRYRERRVRLDQIFKVDISTWI
                     DKNQDAKIIEKFKEILKENEEKFEKVGNILDNCEFDDTGTSSRQEEIDELRSQIVNTF
                     MQLSYNDKLIKRMVYRIRSLVERIEESYESINNLTRIKRYTIDEICMYGRRAKKSDED
                     FQEVLEETGIDPNVFVETLRKIKNARRKIRRVELETKMTGNELIFLLREIDRAERNKE
                     RAQNEMIEANVRLVISIAKHYNNRGLEFLDLIQEGNTGLMRAVEKYDYRKGYKFSTYA
                     TWWIRQAITRAIADQARTIRIPVHMIESINKINRTSRRLMQKLGREPYPEEISEVLDI
                     PVEKIKNIMSISKEPVSLDKPIGHDSEDSILGDFIEDRTIISPERLAERSLLKKQVDE
                     VLKTLTPREERVIRLRFGIDDGYHRTLEEVGNIFQVTRERIRQIEDKALKKLRHPSRA
                     AILQQFLDNFHVK"
     gene            complement(5314..6345)
                     /locus_tag="PLG69_06005"
     CDS             complement(5314..6345)
                     /locus_tag="PLG69_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPI43098.1"
                     /translation="MQTIDALQIMFLVMMLPCLISCMQAKGNVYPLDSKYVKAEELNA
                     KLDKIVQIKPELAEKRIIGFSSAENLPVYALQLGNKNAEKAILVIGQHHGEEVIGVNV
                     SLALAEKLLNNYSQEDKQSHLLENYQVWIIPTLNPEGFRIVSSGILRTKRKNNRDTNN
                     NHKLDLRTDGVDLNRNYPIFWDMDSETEINSPYYKGSEPASESEIQAIIALAQQENFA
                     LAIFLHSSISGAYSEKIYLPARSNDSELFQKTQTLATIYAKEVKKDYLKGTYEVYVGN
                     TSEVGNARNFFFYRMGTPAFLVEIGGQNKRGQSVIHPANAMLEKIVEKNVQAMLKVFE
                     ELEKMKKEK"
     gene            complement(6773..>7279)
                     /locus_tag="PLG69_06010"
     CDS             complement(6773..>7279)
                     /locus_tag="PLG69_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI43099.1"
                     /translation="LYFYLWGGEKIWLAGANGIGKTTLLKIITGELKPTKGTVSYNNF
                     LKFGVYNQNLNTLPGNLTILEYLKQFSDSEMEIRNFMGCLGLTGDIAYQKIEVLSWGE
                     KAKVQLTSLLCGKYNLLLLDEPTNHLDIKTREMLEQALNQYQGAVIFVSHDRAFIQNL
                     ATRKFELK"
BASE COUNT         2357 a         1417 c         1434 g         2071 t
ORIGIN      
        1 aaagcagaaa agaaaggact tttacgcaat cagaaaattc tggcttggct ggattttcgt
       61 tttgcctgtg gacggctttg taacacaata aaggcaaaaa ccccgataat gctatcccgt
      121 ccttgtttga atctaaaatc ggcaaggcat cctcttctga ttttacgcct gggaaattac
      181 cgcaaagtta ttccctttga tttggaactg ggcgaagaga aaaaaattat tattttaagc
      241 ggacccaata ccgggggaaa aacagttctg ctaaaggcag tcggtttgat tactctgatg
      301 gctctttcag gacttcccgt tcctgctgat gccgatagtg aaattggctt ttttagcaat
      361 gtttttgccg atattggaga tgaccagtcc atagaaaatg ctctttccac tttttccgct
      421 cacctggaaa aaataggtaa aatgctcaat aaggcaaaac cggataccct ggttttagta
      481 gatgaaatag gtgctgcaac tgatcctcaa cagggttctg ctttggcaca ggcaattctg
      541 gaaaagtttt tgtctttggg ttgcccggca attgttacaa ctcattacac agccctgaaa
      601 atctttgctg aacagcatcc caggtgtctt aatgcctcaa tgcaatttga taccaaaaac
      661 ctccagccaa cctataaatt tattgccggt ttcccaggtg acagctttgc tattgaagtt
      721 gctgcctctc tgggaattga ccctacctta attgaaagag ctaaagaact tgccggcaac
      781 caaaatgtcc aatttaccga actgctgaaa aaaatgcagg acgaaaaaaa gaaatacagc
      841 gtttcctgct atcaaaatga actgaaaaca cacctgctgg aaaccaaact gcaagaactg
      901 gcaaaaaaag aagaggaatg ggaaaaagaa gtgaaaatac gccgccaaaa tcacttaaag
      961 gaattgcaac aagaactgat cgctcaacaa aaaatatatc agaatgacct cgcagaactg
     1021 aaaacccttt ccaaacagga acgcaagaca ttttcggaat acaaattaca taatatagca
     1081 gttaaaaatg ccgagattca aaaagaactt gacaaagacc tttccgctaa taacttgccc
     1141 gtcagcaatc ctcaacccgg aatgaaggtc tggctttcca attttgaaac ggaagcagtt
     1201 attttgtcta ttgaaggtga acaggtaaaa gtagatatga acggaattac cttcaaaaca
     1261 cctgtgagca cactttttga gcccaaaacg aaaactgaaa caacaaaaat agaacctaaa
     1321 gtgaaagcta atgttgctcc cgtagcaaaa tttgaactga aaattatagg gctaaccttt
     1381 gaagaggcaa aacccttgat agatgaattt ttggataatg ctgccctagc aggcttgcat
     1441 tctttaagaa ttgttcacgg aaaaggaacc ggcgctttaa gaactaaggt tcgggactat
     1501 ctgaaaaaaa gaaaagaggt aaaaggtgtc ggaactccaa gtcctaatga aggtggaacc
     1561 ggagtaacca ttgttactat ctaaacggaa ggtttacctc ctggtcaacg ctaatgaaca
     1621 acagggatgt tgttcttccg agaaaaaatg gatcaaaact taatagatca aattcgtcgt
     1681 gctaacgaca tcgtagatgt tattcaaggt tatcttccct tgaaaagggt gggaagtaac
     1741 tttcgtggtt tatgtccttt tcataatgat tctcgccctt cgctttatgt aagccaatcc
     1801 aaacagattt ttaagtgttt cgcttgtggc aaagccggta atgtaatcgg ttttgtagcg
     1861 gactttgaaa agctgacctt tattgaagcg gtaaaaaaat tggctcagcg tgccggtata
     1921 caagttcctg aatatgaaaa aaccaaagtt gtaaatacta aaagggaaca gctgttaagc
     1981 gtgtataaaa gtgccgcaga gtttttcact tcctcgcttt ttgcgtttgg acagaatgtt
     2041 ttggattatt tgaaaaaacg ttctttttct cccgaaacgg ctaaagaact tcagttgggc
     2101 tatgctttaa acagtgaaaa agccctttta aatcatctcc tgaaagaagg ttatagtgtt
     2161 tctctgctaa aggactccgg actttttggc aattatggcg ggggtttaac agacctcttt
     2221 aaagaccgtt tgatgttccc tattcataat agtttagggg aagttatcgc tttcggcgga
     2281 cgcattctgg aacccaaaga aggtgtggga aaatacataa attcacccgg aacggaatta
     2341 tataccaaag gcaaagaact ttacggttta ttcaaaacca aatacaatat cagcaaagcc
     2401 ggcacagcta ttatctgtga gggctatttt gatttcctgc gtctatacga aaacggcttt
     2461 accaattccg ttgccagctt gggaacagct ctaactgaag atcaaataaa tttactggca
     2521 cgtttctgta atcgggttat aatgttatat gatggcgata gtgcaggaat taaagctgca
     2581 gtaagagccg gacttttatg tttaagtaga ggaatggaag ttttaatagc caatctgcct
     2641 gcagaagaag accccgattc cctgatctta aaacagggta aaatggcaat gcaggaagtt
     2701 gtagataaat caattccctt aatcaatttc ctggcaaccg atccgcgtcc ggaacagcca
     2761 accgcggaaa gaattgaact gattttagat gctttaagga tattaaagga cagaattaaa
     2821 caggagctat tactacaaga ggtctccgat gcctttggaa ttactgtagg tgctttaaac
     2881 agtaaattac gccgtagcag tgcttccgtt tccgctgaat caagtgaaca aactgtgcct
     2941 caatacgaaa gctttgaaga gcgaaatgtt cttgctttag cactaaagga ctatgaatct
     3001 tataaattac ttgccaacga cttagattcc agttatttta ataataaacg ctaccgtcag
     3061 gtatataatt tcctgatcac tcaaaatcta aagtcagagc cctgggaacc ggcagccctg
     3121 cttgataata ttgaaaataa tgaaataaag gaaagcttag ctgaacttct ttttgaggat
     3181 ttacagcaaa tgtgctttga agattgcctc accggcttac gcatccgtaa agtccaaagg
     3241 gacttggaag aaatggatcg cgcaattagc aaagacccta aaaatatgga actcctcaaa
     3301 gagaaagaaa agctggctct aaagtatagg caaatgacca agaaggtcgt aaacaaagtc
     3361 ctctattaga gggtttttcc aaggagcatt aatgattact tacaatgaat gtttgaaaaa
     3421 actggtggaa ttggggaaag aatccggtta cataaccttt aagcagataa atgaaattct
     3481 gccccccagt cccttctttc tggataaggt ggatgatatt atttttgacc tttcccagga
     3541 tggaattgaa atcatagacg aaaccgaaaa acgcatcact aaaagcggag ccgctattcg
     3601 gaaaccatcc acctctaaaa aattcaagtc caaacgctat tacgatgacc ccgttagaat
     3661 gtatttacga gaaatgggtc gtgttcccct tctggataga gaaggagaag tgcgtgttgc
     3721 caaaaagatt gagacctatc agaaaatgat caaccaaaat gtgttcaaat gcggaagcac
     3781 tttaagggaa atgtataact tcctgcatcg ctatcgtgag cgcagagttc ggttagacca
     3841 aattttcaaa gtagatatca gcacttggat agataaaaat caggatgcca aaatcataga
     3901 gaaattcaaa gaaatcctca aagaaaacga agagaaattt gaaaaagtag gaaatatcct
     3961 ggataactgt gaatttgacg ataccggaac ttccagcaga caggaagaaa ttgatgaact
     4021 ccgctctcaa attgtgaata cctttatgca actgtcctat aacgataaac tcattaaacg
     4081 aatggtttac cgtattcgct ccctcgttga acgcattgaa gaaagctatg aaagcatcaa
     4141 taatttaacc cgcattaaac gctataccat tgatgaaatt tgtatgtatg gacgccgtgc
     4201 caaaaaaagc gatgaggact ttcaggaagt tttggaagaa acaggaattg accccaatgt
     4261 ttttgtagag accttgcgca aaattaaaaa tgcccgcagg aaaatccgtc gtgtagaact
     4321 ggaaaccaaa atgaccggta acgaattaat cttcctgcta agggaaattg accgtgccga
     4381 acgcaataaa gaaagagcgc aaaatgaaat gattgaggca aatgtccgcc tcgtaatcag
     4441 tatcgctaaa cactataaca atcgtggact ggaatttttg gacctcattc aggaaggaaa
     4501 taccggactg atgagagccg tagaaaaata tgattaccgt aagggctata aattcagcac
     4561 ttatgccacc tggtggattc gtcaggcaat taccagagct attgcagacc aggcaagaac
     4621 tatccgtatt cctgtacata tgattgaatc catcaataaa attaatagaa ccagccgtcg
     4681 cctgatgcaa aaattggggc gtgaacccta tcctgaagaa atttccgagg tcttggatat
     4741 ccctgtggaa aaaatcaaaa acattatgtc catcagcaaa gagcccgttt ctttggataa
     4801 acccattgga cacgatagcg aagatagtat cttaggggat tttattgagg atagaacaat
     4861 tatttctcca gaacgcctgg cagaacgttc cctcctgaaa aaacaggtgg atgaagttct
     4921 gaaaacatta actccgcgcg aggaacgagt tattcgtctg cgttttggca ttgatgatgg
     4981 ctatcataga accctggaag aagttggcaa tatttttcag gttacccgtg aacgcattcg
     5041 ccaaattgaa gataaggcac tgaaaaaact tcgccatcct tcccgagctg caattttaca
     5101 gcagtttctg gataacttcc atgtgaaata atgtagccag gagacgccat tcctctccat
     5161 ttttgcagtt aaaaagggaa aaggtgaaac aaggcaataa gttgagttgc aatcttgttt
     5221 caggtaactg tattgtcacg agtcctcact gccaaatttg atagaggaaa atggaaagaa
     5281 atattaaagg tagtgacgac tcctgactta catctatttt tccttcttca ttttttccag
     5341 ttcttcaaac actttcagca ttgcctgcac atttttctcc acaatctttt ccagcatagc
     5401 attggcagga tgaataacgc tttgtccgcg tttattttgg cctccgattt ccactaaaaa
     5461 agccggagtt cccatccgat aaaagaaaaa attacgcgca tttcccactt cggaagtatt
     5521 tcctacatag acctcgtaag ttcctttcag atagtccttt ttcacttctt tagcgtaaat
     5581 agtggctaag gtctgtgtct tttgaaaaag ttcagaatca ttactccggg caggaagata
     5641 tattttttcg ctgtaagcac cggaaatgga actatgcaga aaaattgcca aagcaaagtt
     5701 ttcctgttga gccaaagcga taattgcttg tatttcactt tcggaagccg gttccgagcc
     5761 cttataatag ggactgttga tttctgtttc cgagtccata tcccaaaaaa taggataatt
     5821 acgatttaag tccactccat ctgtgcgcag atcaagttta tgattattat tggtatcccg
     5881 gttattttta cgtttagttc taagaattcc ggaacttaca atccgaaacc cttccggatt
     5941 caaagtggga ataatccaaa cctgataatt ctctaaaaga tgggattgct tatcctcttg
     6001 ggaataattg tttaacaact tttccgctaa agcaagggaa acattaacgc caataacttc
     6061 ttctccatga tgttgaccta ttaccaaaat tgctttttcg gcatttttat tccctaattg
     6121 caaggcataa acaggtaaat tttccgcact gctgaaacca ataatccttt tttcagcaag
     6181 ttctggttta atttgcacga ttttatccaa tttagcattc agttcttctg ccttaacata
     6241 tttggaatca agaggatata catttccttt tgcctgcata caagatatca ggcaaggtaa
     6301 catcatcacc agaaacatta tttgtaaagc atctattgtc tgcataaata cctttttatc
     6361 ggaaatgaac ttatcgtcaa ggagaaatac agaaaccgag agttgcaaag tgagagctaa
     6421 agcaatgagg gttccacact aagagccaaa gaaatgaaga atgaatttca gtggaaggca
     6481 catacttata cattttagct ttcttttatt ctcttgcttt ctattatatt acaacaagct
     6541 acttgtaaat tcataaaata ttttcactta ttctgtcaat cttatatttg gattcatctt
     6601 caattttttt tccttctaat tttgaaccac ggaattcact tatagcttgg ttagatagga
     6661 taaagttgga tagatataaa attgaggaga attgtttttg ggctgaaacc tgcaaaattg
     6721 tgagtgaaaa tatttccgta accctttttc tcttggctct ttaccttttg ctttatttca
     6781 gttcaaactt gcgggttgcc aaattctgaa taaaagccct atcgtgcgag acaaaaataa
     6841 ctgcaccctg atattgattt aatgcttgtt ccagcatttc tcgggtttta atatccagat
     6901 ggttggttgg ctcatccaaa aggagaagat tatattttcc acaaagcaaa gaagttagtt
     6961 gcacttttgc tttttcaccc caggaaagaa cttcaatttt ctgataggca atatctccgg
     7021 ttaaccctaa gcatcccata aaattacgaa tttccatttc gctatcggag aactgtttca
     7081 gatactctaa aatagttaaa ttccctggaa gggtgtttaa gttttgatta tagacaccaa
     7141 attttaagaa attgttatag gaaactgttc cttttgttgg tttcagttcg ccggtaatta
     7201 ttttcagcag agtagtttta cctattccgt tagctcctgc aagccagatt ttttcgcctc
     7261 cccaaagata aaaatagag
//
LOCUS       DAOPFL010000083         7244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_13163_length_7244_cov_108.159132, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000083 DAOPFL010000000
VERSION     DAOPFL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13163_length_7244_cov_108.159132"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..3115
                     /locus_tag="PLG69_06015"
     CDS             35..3115
                     /locus_tag="PLG69_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPI43100.1"
                     /translation="MSPITIVEYHPSYAKAIAEMWNNSSEGWNGRVFNSTEEKVLQQE
                     SGTQYLNLYLAVENEKVIGYAKLTRYVEEKGVAYIEMLSVLPSYHGKGIGKELVQKCV
                     LRAAELGYERIDLFTWSANLKAVPLYKKCGFFWERMETQVTHLMNFLPGLLNNELLKS
                     YWNYFHWYNDLKRELNIEPDGRTDNGFDFYDYLWEKDGKRLEVTFERFGRGIVFMKTP
                     DFSIKVDIPNAKPVFGLTYPVHYTLENYQERPITISLQSENDPLVEYNYQQNIVLAEK
                     AELDATFYLKPLERQLTEWEKCPSVNTRICIEDKEITFRTGIKVQFPLTVELRTRDSV
                     FLPKCNYKMFLNVHNHFPVTCFYHLEFPADERLQLSQSKFDFVLNANQKSFIELDFSL
                     NKGLIYNPHFQITAKPEGGKEMQFTTSCYSCFQMLGAKDGIDSPDFIQLLNGNNILYI
                     SKIGEHNFATLVNISTDDLYFPAPEPGKPYSSELKRELPYETVIEKAEDAIQATLKFR
                     STDFPGLDYGIVYRLYDSGLVEYFVTVYALPETEADSWLKIRFYPSGYNLAFAYKNKL
                     YQVGNDLPDVFESNFPQDGFAENWMFIQGVNYTQALIWHPNLTIKPERYYFYGEINLS
                     ELVKSGKNTSAPIRLYQNVFLTPWQVRDLALGRKVPEVIYPTLNLIANQGNPFFTIPC
                     PVEISQILDIEPMGVYSLFSSCLKDAQPQEMQKNEIGERKCSWELNKKPHKPWELLTC
                     KSELYNTVIECKQLIFFSEGEVKNQEKDKLLIADNGCLQITAAKDAQIPGIISLKQKG
                     IEWLENSYPEYAPRSTFNPFAGGIIIKPYAVDAGVLKSESHQVDFIALKDNYGNCWQG
                     LTITTTIDKFEPLKGYIYRQYYLLRPGVPVLAIFAEVVASTGTVGYHTFNFYFHRKHQ
                     QGENDGLFYIQHEDNSWQKIYQTKVQYDFGLDYPTIAMQVADSNYFLQLFKLRRFSTG
                     WVYMDPFIAMLSTYIYTELATIMPTRTEPIFIVMSDELYQKGWLNQLLDISFPV"
     gene            complement(4159..5148)
                     /locus_tag="PLG69_06020"
     CDS             complement(4159..5148)
                     /locus_tag="PLG69_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HPI43101.1"
                     /translation="MIPKRIGFTTSFPVEVIFAAGHIPVDLNNVFITSDSSVKVQKAE
                     LKGFPRTLCSWIKGNYIAALSSQLDLIIGIVEGDCSNSNSLLDMFTEEHLPVYRFSFP
                     ADKNYADLNKEISRLEDYFGVSRKETLKAKKRLDEIRRKLIILDEWTWKEHRVSGLEN
                     HFWLVNSSDFMGNPDRYESELDAFLAVAEKRDPLPAKLRLAYLGVPPIYKNIYETIIE
                     LGGDVLFNEVQRQFSMPYLLTDIVQQYLAYTYPYSVFDRLQDIKPELEKRQIEAVISY
                     TQSFCHLQLDNLLLRKHIDLPFLTLEGDQPEEIDARTLLRLESFFEVHSKIDK"
     gene            complement(5138..7048)
                     /locus_tag="PLG69_06025"
     CDS             complement(5138..7048)
                     /locus_tag="PLG69_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPI43102.1"
                     /translation="MSLIQVIDAGLEFAGNYVLRGINCTLEYNSRIGLIGSNGSGKTT
                     LIKLMLGIIPPSEGKVLRAKKCKIAYLPQNPVLEKDILMGNYIQKSRPDLLSLQQQID
                     ELSSLLQKKHTERTENELKIALDKFQASGGDEFANSMKYVITSLGFSEDDYEKPLYSF
                     SGGEQTRLCLASILLMPYDLLILDEPTNHLDIAMISWLEKYLNNSKAPFLLVSHDRIF
                     LDNTVSTIFQLENTQLSITKGNYSSFAQAKAIEQKTQERQFEKQKKFIAETEDFISKN
                     IAGQKTNQAKSRLKMLSRIEIIQKPQQAKKIKLNIQTAGRSGNDVYTLKDVEFGIPEG
                     PLLAKNVNLQVHYRDRVCIIGPNGCGKTTLLKILLEEREIFSGSLKIGASLEVGYYDQ
                     HQVFLDEDLTVMDTLWQIVPSETRGYVLSYLARFGFQGLDVEKKVEILSGGEKSRLML
                     CVLIHQNPNLLIMDEPTNHLDMEMSDSLLSALQNYRGTIIFVSHDRYFIRQLATKYWV
                     FHKALENNILYPTISEMETNLEEALELAFTIPEPPKAKVNIRERKKKINPYYLEQIHK
                     QIENEQTSITKNHSQIEKINSQLALPSTYSNAMRAKELRQQIDLLLNEIENSKKHIAE
                     LETQYLELSYDS"
BASE COUNT         2181 a         1378 c         1365 g         2320 t
ORIGIN      
        1 ttatattcat tcttcattct ttattggagt ttatatgtcc cctataacaa tagttgaata
       61 tcatccttcc tatgccaaag ccatagctga aatgtggaat aacagcagtg agggttggaa
      121 cggcagagtt tttaacagca cggaagaaaa ggtgttacaa caggaatccg gcacccaata
      181 tctgaatttg tatctggcag tagaaaatga aaaagttatc ggttatgcaa aattaactcg
      241 ctatgtagaa gaaaaaggag ttgcctatat tgaaatgctc tccgttttac cttcgtatca
      301 cggaaaaggt ataggtaaag aattagtcca aaaatgtgtg ttgagagctg cggaattggg
      361 ttatgaacgc attgatttat tcacctggtc tgccaattta aaagctgttc cgctttataa
      421 aaaatgcggc tttttctggg aaagaatgga aactcaggta acgcatctga tgaattttct
      481 tcccggtttg ctcaataatg aattgctaaa atcatattgg aattattttc actggtataa
      541 tgacctgaaa agagaattga atatagagcc agatggcaga actgacaacg gctttgattt
      601 ctatgattat ctttgggaga aggatggtaa acggttagaa gttacttttg agcgtttcgg
      661 tagagggatt gtttttatga agaccccgga cttttctata aaagtagata tcccaaatgc
      721 caaaccggtt tttggcttaa cttatcctgt gcattacact ttggaaaatt atcaggaaag
      781 accgattact atttctctgc aaagtgaaaa tgatcctctg gtggaatata attatcaaca
      841 gaacattgtg ttagcggaaa aagcagaatt ggatgccact ttttatctga aacctttaga
      901 gcggcaatta acggaatggg aaaaatgtcc ttctgttaat acccgtatct gtattgaaga
      961 caaagagata acctttagaa ccggcataaa agtgcaattt cccttaacgg ttgaattgcg
     1021 tacccgggac agtgttttcc ttcctaagtg taattataag atgttcctga atgtccacaa
     1081 tcattttccg gtaacctgtt tttatcatct tgaatttcct gctgatgagc gattacagct
     1141 tagccaaagt aagtttgact ttgttttaaa tgctaatcag aaaagcttta tagagcttga
     1201 tttttccctt aacaagggct taatttataa tccacatttc cagattacag ccaaacctga
     1261 gggtgggaag gaaatgcaat ttacaaccag ctgctattct tgctttcaaa tgttgggtgc
     1321 caaagatgga attgattcac ctgattttat acaacttttg aacggaaaca atatcctcta
     1381 tatatccaaa atcggagaac ataactttgc cacattagta aacataagca ctgatgattt
     1441 atattttccg gcacctgaac cgggaaaacc atattcctca gaactgaaaa gagagctgcc
     1501 ttatgaaacg gttattgaaa aagcggagga tgccattcag gcaactttga agtttcgttc
     1561 aactgatttt ccggggctgg attatggaat cgtttatcgt ctttatgata gtggcttggt
     1621 agaatatttt gttaccgttt atgccctacc tgaaaccgaa gcagacagct ggttaaaaat
     1681 aaggttttac ccttcaggat acaaccttgc tttcgcttat aaaaacaaac tctatcaggt
     1741 aggaaatgat ctccccgatg tttttgaaag taattttcct caggatggtt ttgccgaaaa
     1801 ctggatgttt attcaaggtg taaattatac tcaggcactt atctggcatc ccaatctcac
     1861 tattaaaccg gaaagatact atttctatgg ggagattaat cttagtgaat tggttaaaag
     1921 tggaaagaat acttccgctc cgattcggct ctatcaaaat gtttttctta ctccctggca
     1981 ggttcgggat cttgctttgg gaagaaaagt tcccgaagtt atctatccta ccctgaattt
     2041 aattgctaac cagggaaatc ccttttttac tataccctgc cctgtagaaa taagccaaat
     2101 tctggatatt gagccaatgg gagtttacag tttattttcc agttgtctaa aggatgcaca
     2161 gccccaagaa atgcaaaaaa atgagatagg tgaaaggaaa tgtagctggg aattaaacaa
     2221 aaaaccgcat aaaccctggg aattgttaac ttgcaaaagc gaattatata atactgtaat
     2281 agaatgtaaa cagcttattt tcttttccga aggtgaggtt aaaaatcagg aaaaggataa
     2341 actgctaatt gctgataatg gctgtttaca aattactgct gccaaagatg ctcaaatccc
     2401 aggcattatc tctttgaagc agaaaggaat tgaatggctg gaaaacagtt atcctgaata
     2461 tgcacccaga tccactttca atccttttgc cggaggaata attataaaac cttatgctgt
     2521 agatgctggt gtattaaaat cagaaagcca tcaagtggat tttattgctc ttaaagataa
     2581 ctatggaaat tgctggcagg gactaactat tacgaccaca atagataaat ttgaaccgct
     2641 taagggatat atttaccggc agtattattt attgagacca ggagttccgg ttttagctat
     2701 ttttgctgaa gttgttgctt ccacaggaac tgttggatat catactttta atttttattt
     2761 ccatcggaaa catcagcagg gtgaaaatga cggcttattc tatattcaac acgaagataa
     2821 cagctggcag aaaatttatc agacaaaggt tcagtacgat ttcgggttgg attatccaac
     2881 catagcaatg caggttgctg atagcaatta ctttctgcaa ttatttaagt taagaagatt
     2941 ttctaccggt tgggtatata tggacccctt tatagcaatg ttaagcactt atatttatac
     3001 cgaattggct actataatgc cgacaagaac agagccgata tttatagtaa tgtccgatga
     3061 actgtatcaa aaaggatggc ttaatcagtt gctggatatt agctttccgg tttaatttcc
     3121 tgtagataag ttttgttaat aggtatggga ttttcaggat tttggggatt tacaggattt
     3181 tattgtataa tattttgagg gttgagaggg ttgaaaaagt tgagatggtt gagaaggttg
     3241 agagggttgg aagggtttag aaggtttaaa aggtttagag ggtttagatt tgttaattcc
     3301 tttagagccc gtcagggcga cacaaatata gcgatggtgg cgtaagcccc tcgttaaatg
     3361 taagctccga gttccttttt ccttttattt ttccctctgc caaaagcccg tcagggcgac
     3421 acaaatatag cgatggtggc gtaagcccct cggaaaattc acgatgtaaa cagctggagg
     3481 ttttgactca caattttcac ctgccttaaa tgtcgcctca ccccacaatc attgtcactt
     3541 acaactgctt tacaactggt ctacaaacct aaaaattgtg agttaaaaca aaaattcgca
     3601 caattttcac ctaccttcaa tattcccaca ccccacattc ttttctcact tacaaccaat
     3661 ctgcaactgt ttattaaatc tgagaaattg ggagacaaaa caaaacacgg aataaatatc
     3721 attcccaaag aaatcccaaa attatttcaa taacactccg atatgactcc catatcattc
     3781 ccatatcgcg atatgggaat gatatgggaa tctcatccac tggataacgc aaaaaagcag
     3841 gaattcatac ataactattt tgtttctgtt ttaactcaca atttttcggt ttggagacca
     3901 ataaaaactg tggttgtatg ttaaaaatga ttgcagggtg tgggaatatt gaaagcaggt
     3961 gaaaattgta attcaaaacc tcccgctgtt tacatcgtga attttccgag gggcttacgc
     4021 caccatcgct atatttgtgt cgtcctacgg gcttttttga atctcaacct tctcaaccct
     4081 ctaaaccttc taaaccctct caaccctcca ttatctctca aaaaccttta aattctaatc
     4141 atcctgttca tccaatacct atttatcaat tttactatgc acttcaaaaa agctttccag
     4201 tcgcagcaaa gttctggcat ctatttcttc aggttggtca ccttccagag ttaagaaagg
     4261 caggtctata tgttttctta agagcaagtt atccagttgc agatggcaaa agctttgtgt
     4321 gtaactaatt actgcttcta tttggcgttt ttccagttct ggtttaatat cctgcaaacg
     4381 gtcaaaaacg ctgtagggat aagtataagc caggtattgt tgaactatat ccgttagcag
     4441 atatggcata gaaaattggc gttgcacttc gttaaaaagg acatccccac ccaattctat
     4501 aatcgtttcg taaatatttt tatagattgg aggcactcct aaatatgcta aacgcagttt
     4561 tgcaggtaat gggtctcgtt tttctgctac tgctaaaaat gcatccaatt cgctttcata
     4621 gcggtcggga ttacccataa aatcagaaga attaaccagc caaaaatgat tctccagtcc
     4681 actaacccgg tgttctttcc aagtccattc gtccaagata ataagcttac ggcgaatttc
     4741 atccaagcgt ttttttgcct ttaatgtctc tttgcgggaa acaccgaaat aatcttccag
     4801 ccgggaaatt tccttattca ggtctgcata atttttatct gccggaaaag aaaaacggta
     4861 taccggaaga tgctcttcgg taaacatatc caaaagtgaa ttgctgttgg aacaatcccc
     4921 ttcaacaata ccgataatca aatccaactg agaagaaaga gccgctatat agtttccttt
     4981 aatccagctg caaagggttc tgggaaaacc tttcagttcg gctttttgca cttttaccga
     5041 agaatcactg gtgataaaaa cattatttaa atcaacagga atatgtcctg ccgcaaaaat
     5101 aacttccacc ggaaaagaag tagtaaaacc aattcgttta ggaatcatag ctcagctcca
     5161 gatattgggt ttccagttcg gcaatgtgtt ttttgctgtt ttcaatttcg ttaagcagca
     5221 ggtctatttg ttgtcgtaat tcttttgccc gcatagcatt actgtatgta gaaggaagtg
     5281 ccagctggga atttattttt tcaatttggc tatgattttt tgtaattgat gtctgctcat
     5341 tttcaatttg cttatgaatt tgttccagat aatagggatt gattttcttc tttctttccc
     5401 gtatatttac ttttgctttt ggcggttcgg gaattgtaaa agccagctct aatgcctctt
     5461 ccaggtttgt ttccatttcg ctgatagttg ggtaaagtat attattttcc aacgctttat
     5521 gaaacaccca atattttgta gccagctgac gaataaaata gcgatcatga cttacaaaaa
     5581 taatggttcc ccgatagttt tgcaaagcag aaagcaaact gtcactcatt tccatatcca
     5641 aatgatttgt cggttcatcc ataatcagca gattagggtt ttgatgaatt aaaacacaga
     5701 gcattaaacg cgatttttca cctccgctaa ggatttcaac tttcttttcc acatccaaac
     5761 cctggaaacc aaaacgagcc aaataactta acacataacc tcttgtctcg cttggtacta
     5821 tctgccataa tgtatccatc acggttaaat cttcatccag aaaaacctga tgctgatcgt
     5881 aatagccgac ttctaaggaa gctccaattt taagagaacc ggaaaatatc tcccgttctt
     5941 ccagcaagat ttttagtaaa gttgttttgc cacagccatt gggaccaata atacaaaccc
     6001 tatcacgata gtgaacttgc agatttacat ttttggctaa aagaggtcct tccgggatac
     6061 caaattctac atctttcaag gtataaacat catttccgct tcttcctgct gtttgaatat
     6121 tgagcttaat tttctttgcc tgctgtggtt tttgaattat ttctatgcgg gaaagcattt
     6181 ttaaacggga ttttgcctga ttggttttct gaccggcaat atttttactg ataaaatcct
     6241 cggtttcggc aatgaatttc ttctgttttt caaactgcct ttcctgcgtt ttctgctcta
     6301 ttgctttggc ttgcgcaaaa gaagaataat tccctttagt tatgcttaat tgcgtatttt
     6361 ccagctgaaa aatagtggaa accgtattat ccagaaaaat gcgatcatgc gaaacgagca
     6421 aaaaaggagc tttgctgttg tttaaatatt tttccagcca ggaaatcata gctatatcca
     6481 aatgatttgt gggttcgtcc aaaatcaata aatcataagg cataagcagg atagaagcaa
     6541 ggcacaagcg tgtttgttct cctccgctaa aagaatataa gggtttttca tagtcatctt
     6601 cactaaaacc aagcgaggta attacatatt tcattgaatt ggcaaattca tcaccaccgc
     6661 ttgcttggaa cttatccaaa gctattttta gctcattttc cgtgcgctca gtatgttttt
     6721 tttgcagtaa tgatgagagt tcatctattt gctgttgtaa acttagtaga tcaggacggg
     6781 atttttggat atagttaccc atcaggatgt ccttttccaa aacaggattt tgaggcagat
     6841 aggcaatctt acatttttta gcacgcaaaa ctttgccttc gcttggggga attatgccta
     6901 acatcaattt gataagtgtc gtttttccac tgccgttgga accgataagt cctattctgc
     6961 tgttatattc taaagtgcaa ttaattcccc ttagaacata attaccggca aattcaagtc
     7021 cggcatcaat aacctggatt aagctcaaat ctttctatcc ctttcataat atttttttct
     7081 tgctggaaga cagcattcca gcttgcttaa agctatttta ggaaaacact tctgctgtca
     7141 acctcatttt ttgcttgtca aaatcccgtc cctatttttt aaggtaaatt aaccggttcg
     7201 ggatgtagcg cagtccggct agcgtacttg aatggggttc aagt
//
LOCUS       DAOPFL010000084         7241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_13174_length_7241_cov_91.601308, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000084 DAOPFL010000000
VERSION     DAOPFL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13174_length_7241_cov_91.601308"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..884)
                     /gene="pstA"
                     /locus_tag="PLG69_06030"
     CDS             complement(30..884)
                     /gene="pstA"
                     /locus_tag="PLG69_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPI43103.1"
                     /translation="MKNRKVLNYCGNGLLFLLVGITILVLCIFLVRVFSLGGKVLSWE
                     FLFTAPRNCMQEGGIFPALVGTFWLTIISIGIVIPFGILTAIFLTNYGKPKWLLKVVQ
                     IAIDTLAGTPSIIFGLFGMAIFVNFFAMNVSLISGALTLAILALPLFINSCIEAIKSV
                     PFDFYEASLALGATKRQTITKIVLPTAVPNILTGIIVCIGRVAGETAPIMFTAATFYT
                     RHLPESLKDEVMALPYHIYALMTEGTHPETQVPIAYGTAVVLLLLVLGVSAAAVIIRS
                     SIRRKRKW"
     gene            complement(881..1750)
                     /gene="pstC"
                     /locus_tag="PLG69_06035"
     CDS             complement(881..1750)
                     /gene="pstC"
                     /locus_tag="PLG69_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPI43104.1"
                     /translation="MSRYKEKAFTTITYTAALFSIMVLFALIYGLFREGLPLFNKVSL
                     TEFIFSSSWYPTNAEPEFGAWTFIIGSFCVTIGALIIGIPLGLGSAIFISEIAGNWLK
                     EIVKPVIELLAGIPSVVYGLFGMSAIAPLIRNWLNLDTGLNIFTTSFILGLMIVPIIA
                     SMSEDALTNVPKSIREAALALGSTKMECIFRVIIPAAQRGIIGSILLGLGRAIGETMV
                     VLMVAGGSAQIPKSIFDSVRPLTSTIAAEMGETVMGSLHYQSLYALAILLFVLTFVIN
                     LITEFFLAKRDTK"
     gene            complement(1818..2636)
                     /locus_tag="PLG69_06040"
     CDS             complement(1818..2636)
                     /locus_tag="PLG69_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPI43105.1"
                     /translation="MKTKNYLISIIIMLLAAFNLNAGKGNQITCTGSTTVLPIAQATA
                     EAFMNSHPDINISVRGGGSGVGVAALQNGTTDICNSSRPLKSKEISAAKAKGINPVSY
                     AIANDAISIIVHKDNPLKDISIQQIKDIYTGKIKNWKVLGGPNLPIVVISRDVSSGTY
                     EVFNEKVLAGAKVEGSAQLLASNNAVLSSVSNTPGAIGYVGLGYVNDSVKLISVNGII
                     PSEATVKNGSYILSRKLYMYTNGKAKGDVANYLGFIQSEKGQQIIQELGFINIK"
     gene            complement(2653..3861)
                     /locus_tag="PLG69_06045"
     CDS             complement(2653..3861)
                     /locus_tag="PLG69_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43106.1"
                     /translation="MKKSQFCLNLICLGLLLIPVGLNGLETKLSGEFWVRWISEIAKR
                     KDSNGNYDDKLVSNYLSLERGYLGLETKFTEQVKGRFTVDIFTTDKTYEYQVYSLSDG
                     TLTSSTKSGSIDGAGLKIKYAYVDFGGFIPVKDMTLTAGMQKVYFGSIYDWSYTLIGK
                     APSDEHKVVNSSDLGITLNGFFPSGFGEYQLGIYNGEGYKKIGANLKENTDFAYLGNL
                     RLTPIAGLTVGGSYMLNTVGRDKNLDGETVNTSYEEQKLTDIFTRLAYGPVDLNCEYI
                     VKNVNFPNITDSGKTKEYSATGISIFPCINLKAYLPYDLQLLGCYDVWDESDRLETDK
                     ARSKLNAFTVGINYNFLPDETDNPAMQLQLNYTDKKYDEDKSHSDYADGKKDSSQIYL
                     QLKWKFANTL"
     gene            4295..4894
                     /locus_tag="PLG69_06050"
     CDS             4295..4894
                     /locus_tag="PLG69_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPI43107.1"
                     /translation="MKKGLIVLLAIILVIIICAGWFIGRYNNIQKEKVNVESAWAQVQ
                     NVYQTRYDLVPNLVEIVQGAANFEKSTLTQVTEARAKAGGSLNLPPDALTNPQAFQAF
                     QQSQAGLSDALSRLMVVVERYPELKANQNFLAFQSQLEGIENRIRTERMRYNDAAKKF
                     NQLIVTFPNNIIASIIKVKPFQFFQAEESAQKAPKVQFD"
     gene            5152..5988
                     /locus_tag="PLG69_06055"
     CDS             5152..5988
                     /locus_tag="PLG69_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPI43108.1"
                     /translation="MLLAINVEKLTGDFITPERISLIIRVILILVVGIPLVRLIRSIT
                     KKIVKNRLSPQSEQLVVRSVYYVAILILFITLLNEFGFRLSAILGAAGIFGVAIGFAS
                     QTSFSNIISGIFLISEKPFKVGDVVQVSSNIGTIESIDLLSIKLKTPDNRYVRVPNET
                     MIKTEVINITRYPVRRVDINLSVSYDDDLHKVKEVLLELAESNPLALKDPAPLFFIDK
                     FGDSGIQILFGVWTKKEDFQNLKTSLIIGIKEKFKQENITIPFPHIFIANAEELKNES
                     TS"
     gene            6017..6721
                     /locus_tag="PLG69_06060"
     CDS             6017..6721
                     /locus_tag="PLG69_06060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPI43109.1"
                     /translation="MTTKPQIKAIIFDLDGTLIDSVVDIAGAMNAALKELGYPEHPVE
                     AYKTFIGDGHMELARKVLPEDKRTPENIEALAKKFWDHYDIEWYLHTNIFPGVLYLIQ
                     LAVARKMKLAILSNKPHYFTKKMIRHFFRGAMIRHTKNPFGVYSGEEPNKPKKPDPTV
                     ALELAQRLIVKPQNVALVGDSVVDIQTAKNAGMIAIGAAWGYGNKKDLQAAGADLIFD
                     SPTEMSTYLDSQPLCP"
     gene            6718..7134
                     /locus_tag="PLG69_06065"
     CDS             6718..7134
                     /locus_tag="PLG69_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPI43110.1"
                     /translation="MKSYRRELHFRTDKRREFINITPLVRNVVHESGIKDGLCLVNSM
                     HITSSVFINDDEKGLHSDFETWLEKLAPEKPYSQYRHNGFEDNADAHLKRQIMGREVV
                     VAITNGELDFGTWEQIFYGEFDGMRDKRVLVKIIGE"
BASE COUNT         2357 a         1388 c         1263 g         2233 t
ORIGIN      
        1 gtttaaattt tcagttgata gaatgttatt taccattttc tttttctcct gatggatgaa
       61 cgaataatga ctgctgcggc gctaacacct aaaaccagca ataataaaac aactgctgtc
      121 ccgtaagcaa tgggcacctg tgtttccgga tgagttcctt ccgtcattaa agcataaata
      181 tgataaggta aagccataac ttcatctttt aagctctcag gtaaatgccg tgtataaaaa
      241 gtagctgcag taaacataat aggagctgtt tctcctgcta ctctgccaat acaaacgata
      301 attccggtta aaatattggg tactgcagta ggtaaaacaa ttttcgtaat tgtttgtctt
      361 ttcgttgctc ccaaagctaa agatgcttca taaaaatcaa aaggaacact tttaatggct
      421 tctatgcagg aattgataaa taaaggtaat gccaggatgg ctaaagttaa agctccggaa
      481 attagcgaaa cattcattgc aaaaaagttg acaaaaatag ccattccaaa aaggccaaaa
      541 ataatggaag gagttccagc caatgtatca atggctatct gaaccacttt caacaaccac
      601 tttggtttac cataattagt taaaaatata gctgttaaaa tcccgaaagg aattactatg
      661 ccaatggaaa ttatggtaag ccaaaaagtc cccaccaatg caggaaaaat acctccttct
      721 tgcatacagt ttcggggagc tgtaaataaa aattcccagc ttaaaacctt gccacctaat
      781 gaaaaaactc taactaaaaa gatacataaa accaaaatag taatcccaac caataagaaa
      841 agaagaccat tgccgcaata attcaagacc tttctgttct tcattttgta tcccttttag
      901 cgagaaaaaa ttcggtaatc aggttaatta caaaagtgag cacaaaaagc aatatcgcca
      961 aagcatagag tgattgataa tgtaaactgc ccataaccgt ctcccccatt tctgctgcta
     1021 ttgtagaagt taagggtctt actgaatcaa aaatgctttt aggaatttga gcactgcctc
     1081 ctgccaccat taaaaccacc atcgtttctc caatggctct acccaaacca agcaaaatac
     1141 tgccaattat ccctctttga gctgcgggaa tgataacccg gaaaatacat tccattttcg
     1201 tggaacccaa ggctaaagcc gcttccctta tagatttggg aacattggta agagcatctt
     1261 cactcatact ggcaattatg ggaacaatca ttagccccaa aatgaaggaa gtagtaaata
     1321 tattaagtcc cgtatctaaa ttcaaccaat ttctaatcag aggagctatt gcggacattc
     1381 caaaaagccc ataaacaact gaaggtattc ctgctaaaag ttcaattacg ggcttaacta
     1441 tttcttttaa ccaatttccc gctatttctg agataaaaat tgcagagccc aaacccaagg
     1501 ggattcctat tatcaaagct ccaatggtaa cacaaaaaga accgataata aaagtccagg
     1561 caccgaattc cggttctgca ttcgttggat accagctact gctgaaaata aattcagtta
     1621 aagagacctt attaaaaagc ggcaaaccct ctctaaataa gccatatatt aaagcaaaaa
     1681 gaaccataat agaaaataac gctgcagtat aggtaattgt tgtaaatgcc ttttctttat
     1741 atctactcat ctcatattcc ttgctaacat caggtgaggg aaaaatagca accctcacct
     1801 gttgttctct taatcaatta cttgatgttg atgaatccta attcttgaat tatttgctgt
     1861 cctttttcac tttgaatgaa accaaggtaa tttgctacat ccccttttgc tttaccgtta
     1921 gtatacatat agagttttcg cgaaagtata taactgccat tctttactgt cgcttccgaa
     1981 gggatgattc catttacgct gatcagtttt acgctatcat tcacatatcc caaacccaca
     2041 taaccgatag cccctggagt attacttact gaactcaaga cagcattatt ggaggcaagc
     2101 aattgagcac taccttctac tttcgcacct gccagaactt tttcattaaa gacctcataa
     2161 gttccggaag aaacatcgcg agaaatgact acaatcggta aattgggacc tcccaatact
     2221 ttccagttct tgatcttacc agtgtaaata tcttttatct gctgaatgct aatatcctta
     2281 aggggattgt ccttatgcac tataatgctg attgcatcat tggcaatagc gtaagaaaca
     2341 ggattaattc cctttgcttt agctgccgaa atttcttttg attttaaggg acggctggaa
     2401 ttacagatat ctgtagttcc attttgtaag gcagcaactc ctactcctga acctcctcct
     2461 cgcacagaaa tattgatatc cggatgagaa ttcataaatg cctcagccgt cgcttgggca
     2521 ataggaagaa ctgttgtaga accggtgcag gttatttgat taccctttcc tgcatttaaa
     2581 ttgaatgctg caagtaacat tattattatg ctgatcagat agttttttgt tttcattttt
     2641 atctcctttt ctttaaagtg tattggcaaa tttccatttc agctgcaaat agatttgcga
     2701 tgagtccttt ttcccgtctg cataatcact atgagattta tcttcgtcat acttcttatc
     2761 ggtataattc agctgtaatt gcatagcagg attgtctgtt tcatcgggaa gaaagttata
     2821 atttataccg actgtgaaag cattaagttt acttctggct ttatctgttt ccagacggtc
     2881 tgattcatcc caaacatcat aacaaccaag taattgaaga tcataaggca aatatgcttt
     2941 caaattaata cagggaaata tagagatgcc ggtagcggaa tactcttttg ttttaccgct
     3001 gtcagtaata ttggggaaat ttacattttt aacaatatac tcacagttca aatcaaccgg
     3061 tccataagcc aagcgcgtaa atatatccgt taatttctgt tcttcataag aagtattaac
     3121 cgtttctcca tcaagatttt tatcgcgtcc taccgtatta agcatataag aaccaccaac
     3181 ggttaaaccg gctattggag ttaggcgtaa atttcccaaa taagcaaaat cggtattttc
     3241 ctttagattt gctcctattt tcttataccc ttctccgtta taaatcccca attgatattc
     3301 tccaaaaccg gaagggaaaa agccattcaa ggttataccc aaatcggaag aatttactac
     3361 tttatgttca tcagacggcg ctttgccaat taaagtataa ctccagtcat aaatactacc
     3421 gaaataaact ttttgcattc ctgctgttaa ggtcatatct ttaacgggaa taaaaccacc
     3481 gaagtcaaca taagcatatt taattttaag cccagctccg tctatgcttc cactttttgt
     3541 agaagaagta agtgtgccgt cacttaaaga atatacctga tattcgtagg tcttatcagt
     3601 tgtaaaaata tcaacagtaa aacgaccttt tacctgttcg gtaaattttg tttccaaacc
     3661 cagataacct ctttccaatg aaagatagtt actcactaat ttgtcatcgt aatttccgtt
     3721 gctatccttt cttttagcaa tttccgaaat ccaacgaacc caaaattctc cgcttaattt
     3781 ggtttctaaa ccgtttagtc ctaccgggat aagcaataat cctaaacaaa taaggttcag
     3841 gcaaaactgg gattttttca ttttattctc cttttttctt tttcttggct ttcgcctcta
     3901 atttgattat acttttcttt ggttaagatt ttataagctg aaagttaagt tttctttaca
     3961 tttaggtcta aagttaaaat tcatttactc ccgcctatct ttgttttatt agataactaa
     4021 atgggattat ataacttgta acaagctgag cggcagttat cttttctgta ttaaagccac
     4081 tatcttcatc gcataatcta tgcgaaagac caactacaaa caggtttagc ccttatatta
     4141 attctccttt cggttcaaaa atctcacttt tacttttctc ccttattatg ctgttattct
     4201 ttgtgctttc aatgctccga aagggagaga aaaaattact ggacaaaata aaccatatat
     4261 ttaaaaagga accaaaataa attttggaga taaaatgaag aaaggactga ttgtattact
     4321 cgcaattatc ctcgtgatta taatttgtgc cggttggttt atcgggcgtt ataataacat
     4381 tcaaaaagaa aaggtgaatg tagaaagtgc ttgggctcag gtacaaaatg tttatcaaac
     4441 cagatatgat cttgttccaa atttagtgga aatagttcaa ggagctgcaa atttcgaaaa
     4501 atcaacttta actcaagtaa ctgaagctcg tgcaaaagcc ggtggaagtt taaatcttcc
     4561 tcctgacgca ttaaccaatc cccaggcatt tcaggcattt caacaaagcc aagcaggttt
     4621 gagtgatgcc ctttcccgtt taatggttgt agtggaacgc tatcctgaat tgaaagccaa
     4681 ccagaatttt ctggcttttc aaagccagct ggaaggtatt gaaaacagaa tcagaactga
     4741 aagaatgcgt tataatgatg cagctaaaaa gttcaatcag ctgatagtaa ctttcccgaa
     4801 taatattatt gccagtatta ttaaggttaa gcccttccaa ttcttccagg cagaagaaag
     4861 tgcacagaaa gcccctaaag ttcagtttga ttaaataaca ataagttacg gaatataatt
     4921 tgtttgtcat aatattaaac cgtatataag tactgaattg ttacaagtat agagattagg
     4981 tggttattta attaaagatg gtagttattg ggtgtttgct ctattttaga aaacttgaac
     5041 cacaaaaagt taacagaaga taagtgtttc aaagtaaaat aatatttgga taaatctcca
     5101 aaatgattat cttgttttag gatgccaaaa ttattagagt gaggagaaaa agtgctactg
     5161 gctattaatg tagaaaagct aacaggagat tttattactc cggaaaggat aagtttgata
     5221 attcgggtta ttcttattct ggtggttgga attcctttgg taagattgat tcgttccatt
     5281 actaagaaga tagttaaaaa ccgcctttcc cctcaaagtg aacagttagt tgttcgctcc
     5341 gtttattatg tagcaatttt aattctgttt attaccttac tgaatgaatt tggatttcgg
     5401 ctttctgcca ttttaggagc ggcgggcatt tttggtgtag ctatcggttt tgcttctcag
     5461 accagctttt ccaacattat tagtggcata tttttaatat cggaaaagcc atttaaagta
     5521 ggagatgtgg ttcaggtaag ttccaatatc ggaacaattg aatccataga ccttttatct
     5581 atcaagttga aaacacctga taaccgctat gtgcgagttc ctaatgaaac aatgattaag
     5641 acggaagtta tcaatattac acgctatcct gtgcgccgtg ttgatataaa cctttctgtt
     5701 agctatgatg atgatctgca taaagttaag gaagttcttc tagagcttgc agaaagcaat
     5761 cctctggcat taaaagaccc agctcccttg ttttttatag acaaatttgg tgactcggga
     5821 atccagattc tttttggcgt ttggactaaa aaagaagatt ttcaaaattt gaagacctct
     5881 ttgataattg gtatcaagga gaaatttaaa caggaaaata tcacaattcc cttccctcac
     5941 atattcattg ccaatgctga ggaactaaaa aatgaatcca catcttaaaa taaaataaag
     6001 agtaaggaag tgaagaatga ctacaaaacc tcaaatcaaa gccatcatct ttgatcttga
     6061 tggcacttta attgacagcg ttgttgatat cgcaggtgca atgaatgctg ccttaaaaga
     6121 acttggctac cctgaacatc cggtagaggc atataaaacc tttatcggtg atggacatat
     6181 ggaattggca agaaaagttt taccggaaga taaaagaacc ccggaaaata ttgaagctct
     6241 tgctaaaaaa ttctgggatc attacgatat tgaatggtat ctgcatacca atattttccc
     6301 cggtgtattg tatctcattc aattagctgt agcccgtaaa atgaaactgg caattctttc
     6361 caacaagcca cattatttta cgaaaaaaat gattcgtcat ttcttccggg gagcaatgat
     6421 tcgccatact aaaaatcctt ttggcgttta cagcggtgaa gaacccaata aacccaaaaa
     6481 acctgatccc accgtagctc tggaacttgc ccaacgctta attgtaaaac cgcaaaatgt
     6541 tgctcttgtt ggagatagtg ttgtagatat tcagaccgct aaaaatgcag gaatgattgc
     6601 tataggcgct gcttggggtt atggaaacaa aaaagacctc caagctgctg gagccgattt
     6661 gatttttgac agccctacag agatgtccac ttatttggat tctcaacccc tttgtccatg
     6721 aaaagttatc gccgagaact gcatttcaga accgataaac gcagggaatt tataaacata
     6781 actcctctgg tgagaaatgt cgtacacgaa agcggaataa aagatggctt gtgtttagta
     6841 aattctatgc acattacttc ttccgttttt atcaatgatg acgaaaaagg tctccatagt
     6901 gattttgaaa cctggctgga aaaattagct ccggaaaaac cctactccca atatcgtcat
     6961 aacggttttg aagataatgc cgatgctcat ttgaaaagac aaattatggg cagagaagtt
     7021 gtggttgcca taaccaacgg agaattggat ttcggaacct gggaacaaat attctacggc
     7081 gaatttgacg gtatgcgcga taaaagggtc ttggtaaaaa taatcggaga ataaaaaact
     7141 tatagccgat taagcgttta taagatacaa ttaacacaag gattacagat aattagagga
     7201 agcccgaaag taacttacgg gcttcatcat ttttgatatg t
//
LOCUS       DAOPFL010000085         6630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_14938_length_6630_cov_102.098859, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000085 DAOPFL010000000
VERSION     DAOPFL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14938_length_6630_cov_102.098859"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1070
                     /locus_tag="PLG69_06070"
     CDS             126..1070
                     /locus_tag="PLG69_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPI43111.1"
                     /translation="MSYLYVVIIFAILILAFISRGLIIVRQASVVIIERLGKYYRTLD
                     SGIHIIIPIFDKTRPIHWRYNKSDYRGNVVVVNKVEDRIDLRETVYDFPRQNVITSDN
                     VSININALLYFQITDPYKAVYEIGNLPEAIEKLTQTSLRNVIGELTLQETLTSRDAIN
                     AKLRDILDEATDKWGVKVNRVEMQEILPPEEIRTAMEKEMRAERDKRAKILQADGERE
                     YQIRVADGEKQARIARAEGEAQAKILVADSERKAILLIAEAVKDSKTDPAQFQIALRY
                     VEAFKELVKQGDKTVVLPYESSALLGSVKTLGDIFQKT"
     gene            1279..1758
                     /locus_tag="PLG69_06075"
     CDS             1279..1758
                     /locus_tag="PLG69_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPI43112.1"
                     /translation="MRYYSIFALVLLLLFCACNPKTAEKTETPVDPSALITQIDTIMK
                     ELLTDAENLKTGVLEKYLSNDPTDMYYLGAKVYTSKDLISELKQEYASIANQKLEILQ
                     QKTYILGNEAAVWIANIRGIATNKDKKTIETIYTETWLWQKVNNEWKITHCNKSWQM"
     gene            1876..3075
                     /locus_tag="PLG69_06080"
     CDS             1876..3075
                     /locus_tag="PLG69_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPI43113.1"
                     /translation="MIQDMHQDREFYASELKELLRETLKHSIAGEFSAHSEDPYFYTQ
                     KEYPLQTEIEGLALYPEFIPEVLLNWANLDTSKERKKEDILFLDLETTGLRSNGIFAF
                     MIGLGYYVNEQFIVEQIFLPDPEAEVNSFDRLLELVNEKSLLITFNGKTFDIPVLESR
                     LLYHQIWLNLRAMEHLDLLHLARRLWKNKLPSCALETIEFYILGQIRDKELDIEGGEI
                     PQTYFSFLLNGNPELMQRVFLHNHTDILHTAALFTLICNSIIYPPQGGMDSRIDYHSL
                     AMLYQSQNRIDIAKNILEDMLSAGITDKDVLRDLGFIYKKEKDMESALKCFNIASSLN
                     CPVSMHETCIILEKYYKRYGEALVNAEKLKSYLISRPIVNDQKVAAIDKRIARLKLKL
                     NKVNEVI"
     gene            3076..4122
                     /locus_tag="PLG69_06085"
     CDS             3076..4122
                     /locus_tag="PLG69_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPI43114.1"
                     /translation="MDSVSFGSDNHSGIHPQVLAAIQKANEGFCPAYGDDPLTVSVLT
                     EIKELFGGNCDAWFVITGTGANILCLQSLMHSYNAIFCPQSAHINTDECGAVQKFTQG
                     RLLPIETADGKLTPELIAPHLLGNRDQHHSQAKIISLSQSTEYGTLYTLQELQRLADF
                     AHQNNMFLHIDGARLANAAAALNCSLKEMTKDISADIVSFGGTKNGLLCGEAIISFRP
                     ELTSAMRFYRKQASQLLSKMRFISAQYQAYLEDDLYLKNARTANAMAKLLAERLKEIP
                     ELIVTQEVAVNSVFVILPRQIIAPLQQKYHFYTWNEALNEVRLMCSFNTTETHIESFI
                     QDLKALLVVKSPHC"
     gene            4316..4924
                     /gene="wrbA"
                     /locus_tag="PLG69_06090"
     CDS             4316..4924
                     /gene="wrbA"
                     /locus_tag="PLG69_06090"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HPI43115.1"
                     /translation="MKVLVLFYSAYGHIYKMAEAVAEGARKVEGMEVEIKQVPETLSS
                     EILDKIGATEAKKAFAHIPIAEIEDLTTADAIIFGFPTRFGSMPSQMKTFIDGTGSLW
                     AKGALIGKIGSVFTSTSAQHGGQESTILGFYPVLLHHGMLITGLPYSFQGQGTMDEIS
                     GGSPYGASTVVGDGSRMPSENELEGARYQGWYVASLVAKLHK"
     gene            4946..5137
                     /locus_tag="PLG69_06095"
     CDS             4946..5137
                     /locus_tag="PLG69_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43116.1"
                     /translation="MRGICSSGRYLAYCVAYIKTVITVFLLLPIPVYANLLFDYLNRY
                     IYQYIPITFPYRDMGTIWE"
     gene            5409..6191
                     /gene="tpiA"
                     /locus_tag="PLG69_06100"
     CDS             5409..6191
                     /gene="tpiA"
                     /locus_tag="PLG69_06100"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPI43117.1"
                     /translation="MRKIYIAGNWKMNKGLQETKNFLETVIPELNKLKLENVIPLIAP
                     AYPFLEEALKASYGSCMQVSAQDVSVHSEGAYTGEVSAGMLASLGLKYCIVGHSERRQ
                     YHNETDATVNAKILKLFENHITPLVCIGETLQQREEGKTENVILSQLSGCLKNIKLVS
                     GEEIILAYEPVWAIGTGKTATPSQAQEVHNLIRNWLKSNYSDKVAEKLSILYGGSIKP
                     GNLKELLDCEDIDGGLIGGASLKEEDFLEMVQIGMKSKGQEC"
     gene            6185..>6630
                     /locus_tag="PLG69_06105"
     CDS             6185..>6630
                     /locus_tag="PLG69_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPI43118.1"
                     /translation="MLDIKFIRNNIELVRQAISNKNEKADLDKLLEIDEQRRQLQFAF
                     DNLKAHQNSVSALIAQKKKTGENIENELKAMTKTAEEIKALSAQLSIVNGELEKLLLT
                     IPNIPQEDVPVGKDESANKIIKIWGEPKQFSFTPKDHLELAIQNKLL"
BASE COUNT         2190 a         1177 c         1345 g         1918 t
ORIGIN      
        1 aaaaaaaact ttgcactccc gcactacgaa ctaagaacta taaaaccact ctctcatcct
       61 tcacaatttt tcttgccaaa aaaacacagc tgggaaataa ggaaaaaaat gaaagggagg
      121 taacaatgtc ttacctatat gtggtaatca tttttgcaat cttaattctt gcttttattt
      181 ctcgcggact gataattgtc cgtcaagcaa gtgtagtaat tatagaacgc ttaggaaaat
      241 attatcggac tttggattcc ggaattcata ttatcattcc catttttgat aaaacgcgtc
      301 ctattcattg gcgttataac aaaagtgact atcgcggaaa tgtagttgtg gtcaataaag
      361 tggaagatag gattgacctt agggaaactg tgtatgattt tccccggcag aatgttatca
      421 ccagtgataa tgtttccatc aatattaatg ctttgcttta tttccagatt acagacccct
      481 ataaggcagt gtatgaaatc gggaatttac cggaagcgat tgaaaaactc actcagacct
      541 ctctcagaaa tgttattggg gaattaacct tgcaggagac cctcacttcc cgggatgcta
      601 ttaatgccaa attgcgcgat attttagacg aagcaaccga taaatggggc gtgaaagtaa
      661 accgggtgga aatgcaagaa attttacctc ccgaagaaat tagaacagct atggaaaaag
      721 aaatgcgcgc cgaacgcgat aaacgagcca aaattttgca agcagatgga gaacgcgaat
      781 atcaaatccg agttgcagac ggtgaaaaac aagctcgtat agcccgtgca gaaggtgaag
      841 cacaagccaa aatattagtt gccgatagcg aaaggaaagc aattttactg attgcagaag
      901 ccgttaaaga tagcaaaaca gaccctgctc aatttcaaat tgcgcttaga tatgtggagg
      961 catttaaaga gcttgtaaaa caaggagata agacagttgt tctaccttat gaatcatctg
     1021 cccttttagg cagtgtaaaa acattaggag acattttcca gaagacataa gagatagtat
     1081 acagttgttt agcccaataa tgcagtggaa ataattaata gacatattga gtaattgggt
     1141 tgagaggatt gagaaggtta aaattatttt ctccagacct ctcaacccaa ttcaaacctt
     1201 gacaaaataa ttcccattca gctaaggtat ttttggaagc agaacgaaaa caaccaaata
     1261 accgcttcag gaggtatgat gagatactat tctattttcg ccttggtgtt gctactactt
     1321 ttttgtgctt gcaatccgaa aacagcagaa aagacagaaa cacctgttga tccatcagct
     1381 cttattacac aaattgatac tataatgaag gaattattaa ccgatgctga aaatctgaaa
     1441 accggcgttc tggaaaaata cctgagcaat gatcccacag atatgtatta tttgggtgcg
     1501 aaggtttata ccagcaagga tttaatcagc gaattaaagc aggaatatgc atccatagct
     1561 aatcaaaaat tagaaatcct gcaacagaag acctatattt taggaaatga ggcagccgtc
     1621 tggattgcca acattcgggg aattgctaca aacaaagata aaaagaccat tgaaacaatt
     1681 tacactgaaa cctggttgtg gcagaaagtt aataatgaat ggaaaataac ccattgcaac
     1741 aaatcctggc agatgtagta atattcctat tttattattt atatcagttc ttgaaggaaa
     1801 agtcctttgt tattccggct aaagcaagaa taaaaaagtt atatgcattt tcaaacagaa
     1861 attcagagat aagttatgat tcaagatatg catcaggata gagagtttta cgcttcagaa
     1921 ctgaaagaac tactaagaga aacattaaaa cactctattg caggtgaatt ttctgctcat
     1981 tccgaagacc cttattttta cacccaaaaa gaatatcccc tgcaaacaga aattgagggt
     2041 ttagctcttt atcctgaatt tattccggag gttttgctca attgggcaaa cctggataca
     2101 agcaaagaac ggaagaaaga agacatcctt tttctggatt tggaaacaac cggtttacgc
     2161 agtaacggca tatttgcttt tatgatcggt ttgggttatt atgtaaatga gcagtttatt
     2221 gtggagcaaa tttttcttcc cgatcccgaa gcggaagtga attcttttga tcgcctgctg
     2281 gaactggtaa acgaaaagtc cctactgatt acttttaacg gcaagacctt tgatattcct
     2341 gttttggaat cccgtttgct gtatcatcaa atctggctga atttacgagc aatggaacat
     2401 ttggatttac ttcatcttgc ccgtcgttta tggaaaaaca agcttccttc ctgtgctttg
     2461 gagaccatag agttttatat tctgggtcaa attagagaca aggaactgga tattgaaggt
     2521 ggcgaaattc cgcagaccta tttcagtttt ttgttaaatg gcaatccaga gcttatgcaa
     2581 agggtttttc tgcataatca tacggacatt ttgcatacgg cagctctttt tacacttatt
     2641 tgtaacagta tcatttatcc tcctcaaggg ggaatggaca gtagaattga ttatcattct
     2701 ttggcaatgt tgtatcaaag tcagaaccgc attgatatag ctaaaaacat tttagaggat
     2761 atgctttcag ctggaataac agataaagat gttctgagag acctgggctt tatttataaa
     2821 aaggaaaagg atatggaatc cgctttaaag tgttttaata ttgcctcttc cttaaattgt
     2881 cccgtttcaa tgcacgagac ctgtatcatt ctggaaaaat attataaacg ctatggagaa
     2941 gcccttgtta atgccgaaaa attaaagtcc tatttaatta gccgtccaat cgttaatgac
     3001 caaaaagtag ctgctataga taaacgcatt gcccggctta agcttaagct aaataaggta
     3061 aatgaggtaa tataaatgga ttcagttagt tttggttcgg ataatcacag cggaatacat
     3121 ccccaagttt tagcagccat ccaaaaagct aatgagggat tttgtccggc ttatggagat
     3181 gatcctttaa cagtaagtgt tttaaccgaa attaaagaac ttttcggagg aaattgtgat
     3241 gcctggttcg taattactgg aactggagct aatatattat gtttgcagtc acttatgcac
     3301 tcctataatg ccattttctg tccccaaagt gctcatatca acacagacga atgtggtgct
     3361 gtccaaaaat ttacccaggg acgattactg cccatagaaa cagcggatgg aaaacttact
     3421 ccggaactca ttgcacctca tttattggga aatcgtgatc aacatcattc ccaggcaaaa
     3481 ataatttccc tctctcaaag cacggaatat ggaactttat atactttgca ggaattgcag
     3541 aggttagctg attttgctca tcaaaacaat atgtttttac atattgatgg agcacgtttg
     3601 gcaaatgcgg cggcagcttt gaattgttcc ctgaaagaaa tgactaaaga tatttctgcc
     3661 gatattgtca gttttggagg caccaaaaat ggtctacttt gtggagaggc aattattagt
     3721 tttcgtcccg aattaacttc cgctatgcgt ttttaccgta agcaggcaag tcagctttta
     3781 agtaaaatgc gttttatttc tgctcagtat caggcatatt tggaagatga tttatatttg
     3841 aagaatgcca gaactgcaaa tgcaatggca aaactattgg cagaacggtt aaaagaaata
     3901 ccggaattaa ttgttaccca agaagttgct gttaattctg tgtttgtaat tttgccccgg
     3961 cagataattg caccactaca acagaaatat catttttaca cttggaatga ggcattaaac
     4021 gaagttcgtt tgatgtgcag ttttaataca acagaaacgc atattgagag ttttattcaa
     4081 gacctgaaag ctctattagt ggtcaagagt cctcactgct aaacaaaata gagaatactg
     4141 caaaagttat tttaggggtg acgactcctt acttacagcg taaaaaaaac ctcataaaat
     4201 taaatctgtg ttatctgtgc tatctgtgag agcaaaaaat tatcttccgc tcttgccaag
     4261 ttggattttc cagttaatat atccataaag cttaataata aggagtttac ctaaaatgaa
     4321 agtattagtc ctcttttact ctgcctacgg tcacatttac aaaatggcag aagcagttgc
     4381 ggaaggtgca agaaaagtag aaggaatgga agtggaaata aagcaggttc cggaaacgct
     4441 aagttctgaa attctggata aaataggagc aacggaagca aaaaaggcat tcgctcacat
     4501 tcctattgct gaaattgaag atttaaccac agcggatgct ataattttcg gttttcccac
     4561 gcgttttggc agtatgccct cacaaatgaa aacctttatt gacggaaccg gttctttatg
     4621 ggcaaaaggt gctttaatag gtaaaatcgg cagtgttttt acctctactt cggctcagca
     4681 tgggggtcag gaatccacta ttttgggttt ttatcctgtt cttttgcatc acggtatgtt
     4741 gataactggt ctaccctatt cttttcaagg tcagggaact atggatgaaa tttccggtgg
     4801 ctctccttat ggagcttcta cagttgttgg agatggcagt agaatgcctt cggaaaacga
     4861 attggaaggt gcacggtatc agggttggta tgtggcttcc ttggtagcaa aattgcataa
     4921 ataggatatt tataactatt tcctaatgcg aggtatctgt tccagtggca gatacctcgc
     4981 ttattgtgtt gcctatatca agaccgtaat aactgttttt ttgctcctgc ctattcccgt
     5041 ttatgccaat ttattgtttg attatcttaa ccgctatatt tatcaatata ttcccataac
     5101 tttcccatat cgcgatatgg gaacgatatg ggaatgatat gggaattata tgggtagtga
     5161 taaggactaa aaaagatagc ttgaagcgtc cttaaaaagt agacacggaa tcctaaaaaa
     5221 gggcttaagg gtggttgacc gggaggtaaa ccttccgaga tttaattctc tctacatatt
     5281 aaactttttt cccccatctt tttccgtaaa atcagaattt caaaacccgg ctaaaagaaa
     5341 aatacttgac ctaatatgca aagtaaaatt tgagtgataa aaaggattaa aagaagtgag
     5401 gtatgctaat gcggaagata tatattgccg gtaactggaa gatgaacaaa ggtttacagg
     5461 aaacaaagaa ttttttggag acagtaattc ctgaacttaa taagcttaaa ttggaaaatg
     5521 taataccttt aattgcaccg gcttatccct ttctggaaga agcactgaag gcctcttacg
     5581 gttcctgtat gcaggtttct gctcaggatg tttccgttca ttctgaaggc gcttacaccg
     5641 gagaggtctc agcaggaatg ctggcatctt tgggcttgaa atattgtatt gtaggccatt
     5701 ctgaacgccg tcaataccat aatgaaacag atgcaaccgt aaatgctaaa atactaaagc
     5761 tttttgaaaa tcatataact cctcttgttt gcatcggaga aacattgcaa caacgggaag
     5821 aggggaaaac agaaaatgtt attttaagcc agctttcggg ttgtctgaaa aatataaaac
     5881 tggttagtgg tgaggaaata attttggctt atgaacccgt ttgggcaatt ggaactggaa
     5941 aaactgccac tccttcacag gcacaggaag tgcataattt aattcgtaat tggctgaaaa
     6001 gcaattatag cgataaggtt gccgaaaaac tctccattct ctatggcgga agcatcaaac
     6061 ccggtaacct gaaagaactt ttggattgtg aagatattga tggaggacta attggaggag
     6121 cttccttaaa agaagaggat tttctggaaa tggtgcaaat tggaatgaaa agtaaaggac
     6181 aagaatgtta gatattaagt ttattcgtaa taacatagaa ctcgttaggc aggcaatcag
     6241 caataaaaat gaaaaagcgg atttggataa actgctggaa attgatgaac aaagacgcca
     6301 actgcaattt gcatttgata acttaaaggc acaccaaaac agcgtttctg ccctcattgc
     6361 ccaaaagaaa aagacagggg aaaacattga aaatgaacta aaggcaatga ccaaaacagc
     6421 ggaagaaata aaagctctct ccgctcaatt gagcattgta aacggcgaac tggaaaaatt
     6481 gctgctaacc attcccaata ttccccaaga ggatgttccc gttggaaaag atgaatcagc
     6541 caataaaatc ataaaaattt ggggtgaacc gaaacaattt tcttttactc ccaaagacca
     6601 tctggaactg gctatccaaa ataaactgct
//
LOCUS       DAOPFL010000086         6563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_15160_length_6563_cov_117.164259, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000086 DAOPFL010000000
VERSION     DAOPFL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15160_length_6563_cov_117.164259"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /gene="hisS"
                     /locus_tag="PLG69_06110"
     CDS             complement(<1..1111)
                     /gene="hisS"
                     /locus_tag="PLG69_06110"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPI43119.1"
                     /translation="MKYNIPRGTFDILPEDSYKWQFLQDSFRKMATCFGYEEITTPIF
                     EVAELFERSSGESSDVVQKEMYRFQDKKGREFALRPEGTAPVVRSYVENHLDKLSSRT
                     KLFYLGPMFRYDRPQAGRYRQFYQYGIEFIGSNNPYYDAEVIAVAFLWLNKLGLNNLR
                     LEINSVGCPDCSAVYDKALQEYYRPFLPKLCPDCVKRLETNPRRLLDCKVPSCIEFRK
                     NAPSQMDYLDEPCKKHFSAVCDYLQAMNIPYTINPRIVRGLDYYTNTAFEIIYDGLGA
                     QNSLVGGGRYNGLIAQIGGKSIPAIGFAGGFERLLLALEEEKIELISPSKPDAYLVCV
                     GENTRLQILPYLNALRTNGLIVDYDPDKSSLKAQLK"
     gene            complement(1130..1948)
                     /gene="truA"
                     /locus_tag="PLG69_06115"
     CDS             complement(1130..1948)
                     /gene="truA"
                     /locus_tag="PLG69_06115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPI43120.1"
                     /translation="MARYLIKICYDGTGYCGWQKQKNGLAIQEVMEKTLEIFAGKKTS
                     LIAAGRTDAGVHSLGQYAHFDYSGSMQEKQLLLAFNRYLPDSIKVLEIMKVSPCLNAR
                     YQAYERSYRYRLAKEKTPFNRNYSGFLPHLHLNLEKMQSAVQYLLGKHDFSTFGKSNP
                     EVPNRICELKHISISETERYFIFDFTADRFLHNMVRRIVGTLANISHLDLPPETILEI
                     LENRCPRQNLVITAPASGLYLMDVKYPAKFLDESYNPNFEENGMVNNPLLHSIK"
     gene            complement(1960..2598)
                     /locus_tag="PLG69_06120"
     CDS             complement(1960..2598)
                     /locus_tag="PLG69_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43121.1"
                     /translation="MKWLNHPLSGQHFYLKSAVFLTGLAIGLDSSLRQLLIQLVLILT
                     ILIFEPKLYLSLLNALKKLLPFFAGYWVFATLFGQEFLTTVFFSVQILYLIAVTVYVL
                     GNVKMEQIASQSRWLRKWKPVNSLFYYLFATVFFTRSFFAEYKKMKLGKENNFSLAQL
                     LDVLANVSALSPQISNLVTNLLKFEGEEKTSNPQANYLGIFFLALLVIVHGL"
     gene            complement(2595..5099)
                     /locus_tag="PLG69_06125"
     CDS             complement(2595..5099)
                     /locus_tag="PLG69_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI43122.1"
                     /translation="MKFRDIIICLLCLSGIFACEVNTLFNARNYFKSAQARPLTSNGR
                     PNAQAIDEYTKAIKKCGKIISTDKKGKRVEEAYYLMAKSLYYKGNSAFQAKDQFQNLV
                     IRFPDSKYVPEAYIYIAKILRETNQPKEAEKLLDEFLRNPKYRKHYPEALFVLADFAI
                     KDKDFIKAQHYLERIITEFPKTKEYREAYFLFGKNYYEQKDYDKSLEAFKKMQKARGI
                     DKTIKLEGTYYIGLNELELGQAEKALKTAKGLIKSESRPDKIPFVRLLKARAQFALGD
                     TTEARTEIEFITKNYPRTEGSAGAYYYLAEYYYYNLGDIPKAVLNYNRVRTEFSGSPL
                     AQTAQAKAVALGFVAPRTGLDCETGLSQFLDYHYQAAESFLNVLSLPDSAIARYKIVI
                     SQKDTLIVRRDSLQTAINRNNAILDSLRALLPPPEIPAIDSLGLLEQTELTVTQMDIT
                     SETAFADTLSETIISSPDSLQTFADTLQITESEIPVADSLFVTETISEGDTLATEEEK
                     NKDNGLPDSSLAEEKVELSSPFTETTEPDSLTLAIAKHIQDLEKENARYSQRLEKLEE
                     IIERFESEIVPFCMFAIGSIYHNRSPESPENSKIMAKMQEEYPENKFTKSLYALQNNL
                     PVRLIDPKEEREEQLLDMYFEQIYTAPDSALAGLQELTNSPYHKIKLAANYRLGWYYS
                     FEQIDTLLAKPYLKAVLDEPEAGEYAVVARRFFDGNKFLLRGLFPVVIPQTDTTAVDS
                     TGADIEESIKEWFFPAPAEIYETLPDSLKIYSGIPVLMDSLVKAVSPPEDTMEEKNIS
                     PEMETPDDNIPPLKKNPVPALPEELIPEKKEELPLE"
     gene            complement(5131..>6563)
                     /gene="hutH"
                     /locus_tag="PLG69_06130"
     CDS             complement(5131..>6563)
                     /gene="hutH"
                     /locus_tag="PLG69_06130"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPI43123.1"
                     /translation="DSSKEKVAKCRAYVDKIIENGEIVYGLNTGFGKFSTVIIPEENI
                     AELQLNLIRSHSTNIGEPYSEVQTRAIMLLRIAVLAKGHSGIRLETLETLVQMLNKGI
                     QPIIPMRGSVGASGDLSPLSHLALVLIGEGEAIYKGERLPGASAMQKAGINPVQLAAK
                     EGLALNNGTQVMTALGVLNLLEAERLCRYADIIAAMSIDALKGTPKAFDELIHNLRPH
                     PGQKISAKNIRTLLEGSPLRESHRNCGNVQDAYSLRCTPQVNGAVRDACSYVRRILEI
                     EINSATDNPLIFPDEGKVISGGNFHGEPLAIALDTLGLAISELASISERRIEQMLNPA
                     LSRGLSPFLATRPGIDSGFMIQQMTAASLVSENKVLAHPACVDSIPTSANQEDHISMG
                     SISAIKLCQIVQNVASVLGIELLIACSALDQRQISTSPVLEKVKSALRENVAPLQGDR
                     VMYPDVNKAIELVRSKRLDEICPEIG"
BASE COUNT         2011 a         1354 c         1203 g         1995 t
ORIGIN      
        1 attttaattg ggctttcaga gagcttttat cgggatcgta atccacaata agtccgttag
       61 ttcttagggc attcagataa ggcagaattt gcagacgcgt attttctcca acgcaaacaa
      121 gataagcatc cggtttagaa ggtgagataa gttctatttt ctcttcttcc aaagccagaa
      181 gaaggcgttc aaaacccccg gcaaaaccaa tagccggaat actttttcct ccgatttgtg
      241 caatcaaacc attgtatctt ccacctccaa caagggaatt ttgtgcgccc aaaccatcat
      301 agataatttc aaaagcagtg tttgtataat aatccaatcc gcgcactatt cttggattga
      361 tggtataagg aatattcata gcttgcaagt agtcacaaac tgcagaaaaa tgtttcttac
      421 agggctcatc cagataatcc atttgcgaag gtgcgttctt tctaaattct atacaggaag
      481 gaactttgca gtccagtaaa cgacggggat ttgtttccaa ccgtttcaca caatcagggc
      541 ataatttggg caaaaaaggt ctataatatt cctgcaatgc cttatcataa acagcagaac
      601 aatcaggaca gcccacacta ttaatttcca aacgcaagtt attcaaaccc aatttattaa
      661 gccacaaaaa agcaaccgct atcacttctg catcgtaata aggattgtta ctgccgataa
      721 attctattcc gtattgataa aattgacgat atcttcctgc ctgaggacga tcgtaacgaa
      781 acataggtcc caaataaaat aattttgtgc gggaagaaag tttatccaga tgattttcta
      841 catagctacg aaccacagga gcagtgcctt caggacgcaa agcaaattcc ctgccttttt
      901 tatcctgaaa acgatacatt tctttttgga caacatcaga actttctccg gaactgcgtt
      961 caaagagttc agccacctca aaaatgggag tagttatttc ttcatagcca aaacaagttg
     1021 ccattttacg aaagctatcc tgtaaaaatt gccatttata gctgtcttca ggtagaatat
     1081 caaaagttcc gcgcgggata ttgtatttca tttttatttc ctcttccttt tattttattg
     1141 aatgtagcaa agggttattt accatcccat tttcctcaaa attaggattg taagattcgt
     1201 caagaaactt tgccggatat ttaacatcca tcaggtaaag tcctgatgca ggagcagtta
     1261 tcactaaatt ttggcgaggg catctattct ccaatatttc cagaatcgtt tccggaggca
     1321 aatccaaatg tgatatatta gccaaagttc ctacaattct ccgaaccata ttatgcaaaa
     1381 agcggtcagc tgtaaaatca aagataaaat agcgttccgt ttcgcttata cttatgtgtt
     1441 taagttcaca aatacgatta ggaacttccg gattgctttt cccgaatgtg gaaaaatcgt
     1501 gttttcctaa aaggtattgt acggcagatt gcattttctc caggtttaaa tgcaagtgag
     1561 gcaagaaacc gctgtaattt ctgttaaaag gtgttttttc tttagccaaa cgatagcgat
     1621 aactgcgttc ataggcctgg taacgagcat ttaaacaagg gctaactttc attatttcca
     1681 gcactttaat agaatcaggc aaatatctgt taaaagccaa aagcaattgc ttttcctgca
     1741 tagaaccact ataatcaaaa tgagcatatt ggcctaaaga atgaactcct gcatcggttc
     1801 ttccagcagc aattaaggaa gtttttttac cggcaaatat ctccagcgtt ttttccataa
     1861 cttcctgaat ggcaagtccg tttttctgtt tttgccatcc gcaatagcca gtgccatcat
     1921 aacatatttt tattaagtat cttgccatag ccaacctttt tataagccgt gcactataac
     1981 taatagggct aagaagaaga tacccaaata gttagcttga ggattgctgg ttttttcttc
     2041 cccttcaaat tttagcagat tggtaactaa attgctaatt tgaggagaga gtgcagatac
     2101 atttgctaaa acatccaaaa gttgagccag ggaaaaatta ttttctttac ccaatttcat
     2161 cttcttgtat tcggcaaaga aagacctggt aaagaatact gtagcgaaga gataatagaa
     2221 caatgaattt accggtttcc attttcttag ccatctgctt tggcttgcta tctgttccat
     2281 tttcacattt cccaacacat aaaccgtaac agctattaga tataaaattt ggacactgaa
     2341 aaacacagta gtaagaaatt cctgaccaaa aagagtagca aaaacccaat aaccggcaaa
     2401 aaaaggcagt aattttttca aggcattgag cagagaaagg tatagctttg gctcaaaaat
     2461 cagaatagtc aaaatcaata ccaactgtat taaaagctga cgcagggaag aatccagtcc
     2521 gatagccagc ccggttaaaa aaacagctga ttttagatag aaatgctgtc ccgaaagggg
     2581 atgattcaac cacttcattc aagtggcagt tcctcttttt tttccggaat cagctcttcc
     2641 ggtaacgcag gaacaggatt ttttttcagt ggaggaatat tatcatcggg tgtttccatt
     2701 tcgggagata tgtttttctc ttccatcgtg tcttcaggcg gagaaacagc ttttaccaaa
     2761 gaatccatca gcacaggtat gccactataa attttcaagc tgtccggcag ggtctcataa
     2821 atttctgcag gagcaggaaa aaaccattcc ttaatgcttt cttctatatc agctccagtg
     2881 gaatcaactg cagtagtatc agtttgagga ataacaacag gaaataaacc tcgcagaagg
     2941 aacttattgc catcaaagaa tctacgggca acaacagcat attcgccggc ttcaggttcg
     3001 tccaaaacag ctttcaaata gggctttgca agcagagtat caatttgttc aaaactgtaa
     3061 taccagccca aacggtaatt agcagccagc ttaattttat ggtagggaga gtttgtaagt
     3121 tcctgtaaac cagcaagagc ggaatcagga gcagtataaa tttgttcaaa atacatatct
     3181 aaaagctgtt cttccctttc ttctttgggg tcaattaacc gcaccggcaa attattctgc
     3241 agagcataaa gggatttggt aaatttattt tccggatatt cttcctgcat tttagccata
     3301 attttactat tttcaggact ttcaggagaa cgattatgat agatggaacc aatagcaaac
     3361 atacagaaag gaactatttc actttcaaaa cgctcaataa tttcttccag tttttccagt
     3421 cgctggctgt atcttgcgtt ttccttttcc aggtcttgaa tatgtttagc aattgccaaa
     3481 gttaaagaat ctggctctgt tgtttcagta aacggagacg ataattcaac tttttcttct
     3541 gccaaagagg aatctggaag accgttatct ttattttttt cttcttcagt agctaaggta
     3601 tcgccctcag aaattgtctc tgtcacaaat aaactatccg ctacaggaat ttccgattcc
     3661 gttatttgta atgtatccgc aaaagtttgc aaactatccg gactgctgat aattgtttca
     3721 gatagggtat cagcaaaagc agtttcggat gttatatcca tttgtgtcac agtaagttcc
     3781 gtctgttcta aaagtcctaa actgtctatt gcaggaattt ccggtggcgg taaaagcgct
     3841 ctaagcgaat ccagaatagc attatttctg tttattgccg tctgtaaaga atcacgtctg
     3901 acaattaaag tatctttttg agaaataaca attttgtaac gggcaatagc agaatcgggc
     3961 aaagaaagga cattcaaaaa actttccgcc gcctgataat gataatccaa aaattgagaa
     4021 agtcctgttt cacagtcaag acctgtgcgc ggagctacaa atcctaaggc aaccgctttt
     4081 gcctgagctg tttgagcaag tggagaaccc gaaaattcag tgcgtacccg attgtagttc
     4141 aaaaccgctt taggaatatc acccagatta taataataat attctgccag gtaataatag
     4201 gcacctgcag aaccctcagt acgaggataa tttttagtaa tgaattcaat ttcagttctt
     4261 gcctcggttg tatcgcccaa agcaaattga gctcttgcct tcagcaaacg gacaaaggga
     4321 attttatcgg gacgagattc cgattttatc aagcccttag ctgtctttag tgctttttct
     4381 gcttgtccaa gttccagttc attcaaaccg atgtaataag ttccctcaag ctttatagtt
     4441 ttatcaatac cgcgcgcttt ttgcattttt ttaaatgctt ccagcgattt atcgtaatcc
     4501 ttttgttcgt aataattctt gccaaaaagg aaatatgctt cgcggtattc ttttgttttg
     4561 ggaaattccg taattatcct ttccagataa tgttgagctt taataaagtc cttatcctta
     4621 atcgcaaagt cggctaaaac gaataatgct tcaggataat gtttacgata tttaggattg
     4681 cggaggaatt catccagcaa tttttccgct tctttgggct gattcgtttc tcttaaaatt
     4741 ttggcaatat aaatatatgc ctcgggaaca tatttactat cgggaaaacg gataacaagg
     4801 ttttgaaact ggtcttttgc ctgaaaagca ctattgcctt tataataaag ggattttgcc
     4861 atcagataat atgcttcttc tactcttttt ccctttttat cggtagaaat aattttcccg
     4921 cattttttta tcgctttcgt atattcatca atagcttgag cattaggtct gccattactg
     4981 gttaaaggtc ttgcttgtgc cgatttaaaa taatttcggg cattgaacaa agtattcacc
     5041 tcgcaggcaa aaatgccgga aaggcaaagc agacagataa ttatatccct gaatttcata
     5101 gttcaatagc ccctaaacgg caaatttttg tcaaccaatt tcaggacaaa tctcgtccaa
     5161 ccgcttggaa cgaaccaact caattgcttt atttacatca ggatacataa cccggtcacc
     5221 ttgtaaagga gcaacatttt ctcttaaagc cgattttact ttttccaaaa caggagaagt
     5281 agatatttgc ctttgatcca gtgcagaaca ggcaatcagc aattcaattc ccagaacgga
     5341 agccacattt tgcactattt ggcaaagttt tatagccgaa attgatccca tagaaatatg
     5401 atcttcctga ttggcagaag tgggaatgga atctacacag gcaggatgag ccagcacttt
     5461 attttccgaa accagcgatg ctgccgtcat ttgctgaatc ataaaaccgg aatcaattcc
     5521 cggacgcgtt gctaaaaaag gagaaagacc tctggaaaga gcaggattta acatctgttc
     5581 aatgcggcgt tcggaaattg aggcaagttc actaattgcc aaacctaaag tatccaatgc
     5641 gatagctaag ggttcaccat gaaaattgcc cccggaaata accttaccct catccggaaa
     5701 aataagcgga ttatccgttg ctgaatttat ttctatttcc aaaattcttc gcacataaga
     5761 acaggcatct ctcactgcac cattaacttg aggtgtgcat cgcaaactgt aagcatcttg
     5821 aacattgccg caattacgat gagattcccg caaaggactt ccctccaaaa gggttctgat
     5881 attttttgca gaaattttct gacctggatg tggtcttaaa ttatgaatca attcatcaaa
     5941 tgctttagga gtccctttta aagcatctat gctcatagcg gcaatgatat cggcataacg
     6001 gcataaacgc tccgcttcca aaaggtttaa aacacccaaa gcagtcataa cttgagtgcc
     6061 attattaaga gcaagtcctt ctttagcagc aagttggaca gggtttatgc ccgctttttg
     6121 cattgctgaa gcacccggaa gacgctcacc tttgtaaatt gcttcacctt caccaattag
     6181 cactaatgcc aaatgtgaaa gcggagaaag atctccactt gcgccaactg aaccccgcat
     6241 tggaattatt ggctgaatac ctttattcaa catttgcacc agggtttcca atgtctctaa
     6301 acgaattccg gaatgccctt tggctaaaac tgcaatcctt aaaagcatta tagcccgcgt
     6361 ttgcacttcg gaatagggtt ctccgatgtt ggttgaatga ctgcgaatta aatttaattg
     6421 tagttcagct atgttttctt caggaataat tacagtgctg aatttgccga aaccagtatt
     6481 cagcccatag acaatttcgc cattttcaat tattttatct acataagcac ggcatttagc
     6541 cactttttcc ttactggaat cag
//
LOCUS       DAOPFL010000087         6347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_15937_length_6347_cov_97.695645, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000087 DAOPFL010000000
VERSION     DAOPFL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15937_length_6347_cov_97.695645"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            666..4586
                     /locus_tag="PLG69_06135"
     CDS             666..4586
                     /locus_tag="PLG69_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43124.1"
                     /translation="MKRIWIIILTLLLVFPLAGVILETASLKHFLYGNEPNCAYDNWI
                     SHLAEGIAIQGYNTYAPYDRQTNGFGDFVVPNDDQLTAWNYIVGLFLAGYFDEAQTTI
                     NAVGFPYQVVLFYDTDSGITYRMLREVPNPEYYDDNGTDDTYDDENGAFAYGWGLYLY
                     NPLGSRPVIVTVPHPCDDFPTPAFALEAFQIWDAQFLLINGAGREVRWTNQGSYNNSK
                     SLSDPTRLYNHPFNVCYKMFADLIRTEFNIREFSPQIHSYDWNYHPGYPNVQISAGYN
                     RLCPNLPIRDLSSRKLDMINKGHHIMIPANTVGFHREVYLNDFYGVNYDLYPFLFDDG
                     EHCYEVNNYIDLPAYSQNHQMLYTQSGTTDYDVYDPFLHTEMDELPNSYELTENTYKW
                     FYGWNEALQCWNFDHLFDNFRMYYLRWVYDLESVMDEMFAMNDGLIPPTPVGFSIQNQ
                     SLNSITLNWQKVDCYDFDTFEILYSINPIDGSNYQIYNRNNNAILASPYCESVTVPGL
                     SSSNSYFFKIRSKDKNGNYSELSNQITTIPAPATIYTFTAHGLDNEVRLFWGVSGQTN
                     NLGYKVYRKAPTQADYTLIDSYLTNPSLANTSVSNYTYWDYNVTNGQNWDYIISCTNV
                     NNQEFFYNYPVSAAVRPIHNLTLTNSTATLVDTIYFAQNPYASDSQDAYYDITKSNPS
                     GSSYVWSAFWEAYWGSNGTALSREVKGGYDTALDLKTWTIRIRSTEVNTPLYLSASDN
                     FSRAEKLYIYDSGNGTWHNLFSGPYQFMVPNNNVRTMTLYWGNLQPKISHINQNNQLF
                     QGGNNITFQWSAQNSFLIDHLDLYVKNESDSLFLTGNIPGNQNSWIYNIPPNVDMQKA
                     RFYINCYAVDGLIQTFVSPYTFALVPRMILHSNEAGWQTRSQIWPDLTPTVETLFGNG
                     AIALTPANDGTWQENDDLLFGIAYWINAPAVNFFNSTAEICPTEINSFPLQPGWNFIA
                     NPHYCSYPVQSLRFLVGTNPFLYSEMIAQNLVSRAVFVYREGKFQSVDTILPFEAFYI
                     KYYGDQSLNTYLRFYPYFEAPEIDPPDNFWQFKVNVSSAGSNADEFVLGTNPIATDGY
                     DFYLDLPSAPEKPFPGLSVFITREAPEDIFFRDKKLSAEFREAFSPINQQEKIWHFKL
                     VCNSTSPVEFNLSDIDLPNDYTVQFYLGSEYYNYEHTNSFTFFPNEPGTYTGLIKVAN
                     YPVSVSDLVQKPISNITIYPNPFNPTTTIAFNTFATQEVELLLYNLKGQKVRTLYQGI
                     LNGGEHKLIWDGKDNNGRTVSSGIYFAKIQAGKYTQIRKMMLIK"
     gene            4641..4784
                     /locus_tag="PLG69_06140"
     CDS             4641..4784
                     /locus_tag="PLG69_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43125.1"
                     /translation="MQKIMGWQISSTFISWKNACNAGFGENLAEEKKAYFQTHNLAVI
                     YNV"
     gene            4800..5990
                     /locus_tag="PLG69_06145"
     CDS             4800..5990
                     /locus_tag="PLG69_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPI43126.1"
                     /translation="MQAGRIYLDNCVTTCPAPEVLTEMQPYWEKKFWFPGTFISTGES
                     TNSLINKAQNTIAETLEASAQEIHFTSGGTLANNIAIKGLANAYKQQGKHIIVSVVDY
                     PDLLTNAAYLEKNGFEVTYLSADSEGFISLEELKSAIRKDTILFMTTAVNHTVGTIQP
                     FAEIKAILESADHKIYYHLDAGQGYGKIPLKVNELGIDTMSISAHKIHGPQGIGALYQ
                     RTGTKLAQLIHGVKRVDNLQTGGLSIALIAGFAKAVELTFRDFEANTAYLRELSNYLL
                     DRIEKEIPYVELNGPRGEKRAPHNINVSIDFIEGEAITMMLDQKGITVATGSACASEG
                     LKANYVLMAMGKTHIQSHGSMKFTVSRYTTKAELDETVQALAEITQELRNRSPLYLAM
                     TKEK"
     gene            5992..>6347
                     /locus_tag="PLG69_06150"
     CDS             5992..>6347
                     /locus_tag="PLG69_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPI43127.1"
                     /translation="MQYSQKVLDHFMHPHNVGKMENPDAVATEGSPACGDQVTVYLKV
                     DDKTKTITDISFLSYGCASNIATASIITDMAKGKTLEEAKKITWRDAMDALDGLPPVK
                     VHCSVLAADTLQSAIS"
BASE COUNT         1957 a         1194 c         1196 g         2000 t
ORIGIN      
        1 gggaagatat tctcggtatg gatgcttaaa cttccttttt cttttgtttt cttgtctgcc
       61 attatgttta aatctcctta ttttaattca ttgtgtttct ggagataaaa taatcggtct
      121 tggcttattg tcaagatttt ttagcagtcc cataacttga ttgctaaatt tgtgcaaaac
      181 aaatcaggac tcgttatgcg gaaggttgat atcctcatca atcaccaaaa tcggaagggt
      241 taaatgactc ccggcttgca gtttacctgc ctatttttcc cggacaaaac cttcttataa
      301 ccctgtaaca aataagtaac aatcccgtaa cgagattact gcagtgttag ggaagtgtta
      361 cggaagtgtt acggggtctt cttggaatta actgatttgc aggaagttac aaatttggaa
      421 ataaataccc taaagaacat aaatttatca cgcagaatgc gctgattatg ctgattttat
      481 ttgtcgtgcc gtcagtagtt ataagcaccc aaaaaagata tgttcagaaa taaatttgtg
      541 aagacgctaa taactcctga cttacaaggg atattgttct tcctgtatat tattcttgac
      601 taaatttgaa agaactcaat taatgtattt tataagttct tagaccataa aaattgggag
      661 tattgatgaa aaggatttgg ataataatac taaccctcct gctggttttt cctctggcag
      721 gagtaattct agaaaccgct tcgctaaagc attttcttta tggaaatgaa cccaattgcg
      781 cttacgacaa ttggatttcc catttagctg aaggtattgc aattcagggc tacaatactt
      841 atgctcctta cgatagacaa accaacggtt ttggtgattt cgtagttcct aatgatgatc
      901 aattaactgc ttggaattat attgtgggct tatttttagc cggctacttt gatgaagcgc
      961 agacaacgat taatgctgtc ggatttcctt atcaggttgt tcttttttat gatacggaca
     1021 gtggcataac ttatagaatg ttgcgtgaag ttcccaatcc ggaatattat gatgataacg
     1081 gaactgatga tacttatgat gatgaaaacg gtgcttttgc ctacggttgg ggtttgtatc
     1141 tttacaatcc tttagggagt agacctgtta ttgtaactgt tcctcatcct tgtgatgatt
     1201 ttcctactcc tgcttttgct ttagaggcat ttcaaatttg ggatgcccaa tttttattga
     1261 tcaatggagc aggtagagaa gtaagatgga caaatcaagg tagttataat aattccaaat
     1321 ccttatctga tcctacgcgc ttatacaatc atcctttcaa tgtctgttat aaaatgtttg
     1381 cagaccttat tagaaccgag tttaatatcc gggaattttc tcctcaaata catagttacg
     1441 attggaatta tcatccgggt tatcctaatg tccaaatttc agcaggatac aatcgtctct
     1501 gtcccaacct tcctattaga gatctatcca gccgaaaact ggatatgatt aataagggtc
     1561 accatataat gattccagct aatacggtag gttttcacag agaagtttat ctgaatgatt
     1621 tttatggtgt taattatgat ctctatccgt ttctttttga tgatggtgaa cactgttatg
     1681 aggtaaataa ctatattgat ttgcctgcct attcccaaaa tcatcaaatg ctgtatactc
     1741 aatccggaac aacagattat gatgtttatg atccctttct ccatacagag atggatgaat
     1801 tgcccaattc ttatgaatta accgaaaata cctataaatg gttttacggc tggaatgaag
     1861 cattgcaatg ctggaatttt gaccatcttt ttgataactt taggatgtat tatctgcgtt
     1921 gggtttacga tttggaaagc gttatggacg aaatgtttgc aatgaatgat ggcttaattc
     1981 ctcctactcc agtaggtttt tccattcaaa atcaatccct taattccatc accttaaatt
     2041 ggcaaaaggt agattgttac gattttgata cctttgaaat actatattcc ataaatccta
     2101 ttgatggttc taattatcag atttacaatc ggaacaataa tgcaatttta gcttcaccct
     2161 attgtgaaag tgttacggtt ccgggattga gttcttctaa tagctatttc ttcaaaatcc
     2221 gctcaaaaga taaaaacgga aattattcgg aactttccaa tcagataact acaattcccg
     2281 ctccagccac tatttatact tttactgctc acggtttgga taatgaagtc cgcctttttt
     2341 ggggtgtttc aggacaaact aataatctgg gatataaggt ttataggaag gcaccaaccc
     2401 aagctgacta cactttaatt gattcttacc ttaccaatcc ctctctggca aatacatctg
     2461 tatctaacta tacctattgg gattataatg ttactaacgg tcagaactgg gattatataa
     2521 tcagctgcac gaatgtaaat aaccaggaat ttttctataa ttatcctgtt tctgcagctg
     2581 ttagacctat tcataattta actcttacta actctactgc cacgttggta gatactattt
     2641 actttgctca aaatccatat gcttccgata gccaggatgc ctattatgac ataaccaagt
     2701 ccaatccttc cggttcaagt tatgtatggt ctgctttttg ggaagcatat tggggttcca
     2761 atggaacagc actttcccgg gaagttaaag gtggctatga tactgcttta gacctgaaaa
     2821 cctggacgat tagaattcgc tctacggaag tgaatacacc tctctattta tctgcttcag
     2881 ataatttcag ccgggcggaa aaactgtata tttacgattc gggaaacgga acttggcata
     2941 acttatttag cggaccttat caatttatgg tccccaataa caatgtaaga actatgactt
     3001 tgtattgggg caacctgcaa cccaaaattt ctcatattaa ccaaaataac cagctttttc
     3061 aaggtggcaa taatattacc ttccagtgga gcgcgcaaaa cagttttctg attgaccatc
     3121 tggatttgta tgttaaaaat gaaagtgaca gcttgttttt aacaggaaat attccgggaa
     3181 accaaaacag ctggatatac aatattcccc ccaatgtaga tatgcaaaaa gcccgttttt
     3241 atatcaattg ttatgctgtt gatggtttaa tccaaacatt tgtatcgcca tatacttttg
     3301 ctcttgttcc caggatgatt ttacactcta atgaagctgg ctggcaaacg cgctcccaaa
     3361 tttggcccga tttaactcct acggtagaaa ctcttttcgg taatggagcc attgctttaa
     3421 ctccagctaa tgatggaact tggcaggaaa atgatgatct cctgtttggt attgcctatt
     3481 ggataaatgc accagcagta aatttcttta attccacggc tgaaatttgt ccaactgaaa
     3541 ttaactcttt tccgctgcaa ccgggttgga attttatagc caatcctcat tattgcagtt
     3601 atccggttca gagtttacgc tttctggtag gaacaaatcc ttttctctac agtgaaatga
     3661 tagctcaaaa ccttgtttcc cgagctgtat ttgtctatcg ggagggaaag tttcagtcag
     3721 tagacacaat tttacccttt gaggcatttt atatcaaata ttatggtgac cagtccttaa
     3781 atacttatct tcgtttctat ccctattttg aagcaccgga aattgaccca cctgataatt
     3841 tctggcaatt taaagtaaat gtttcttctg ccggtagcaa tgctgatgaa ttcgttttgg
     3901 gaacaaatcc aatcgccacc gatggttatg atttttacct ggatttacct tctgctcccg
     3961 agaaaccatt tcccggcttg agtgttttta taacaagaga agcacccgaa gatatctttt
     4021 ttagggataa aaaactttca gctgaattta gagaagcatt ttctccaata aaccagcagg
     4081 aaaaaatctg gcacttcaaa cttgtttgta attccacctc tcctgtggaa tttaatctta
     4141 gcgatataga cctgcccaat gattataccg ttcaatttta tcttggttca gaatactata
     4201 actatgaaca tacaaatagt tttaccttct ttccaaatga acctggaacc tatacgggat
     4261 tgatcaaggt tgcaaactat cctgttagcg taagtgactt ggttcagaaa ccaatttcca
     4321 atataaccat ttatcccaat ccctttaatc cgacaactac tattgccttt aatacttttg
     4381 caactcagga agttgaactc ttgttatata accttaaagg acaaaaagtt cgcacccttt
     4441 accagggaat attaaacggt ggagaacata aattgatttg ggatggaaaa gataacaacg
     4501 gcagaactgt aagcagcgga atttattttg ccaaaataca ggcaggaaag tatactcaaa
     4561 ttcggaaaat gatgttgatt aaataagagc tgggggttta tgcatagacc attttgaggg
     4621 gataaaccgg aaaaataaag atgcagaaaa taatgggttg gcaaatttcc agcactttta
     4681 tcagctggaa aaatgcctgt aacgctggtt tcggggaaaa tttagcagaa gaaaagaagg
     4741 catactttca gacacataat ttggctgtaa tctataatgt ttgaaaagga gaaaaaataa
     4801 tgcaagcggg aagaatatat cttgataatt gcgtaactac ctgtcctgca ccggaagttt
     4861 taacagaaat gcaaccctat tgggaaaaaa agttttggtt tccgggaact tttatcagca
     4921 cgggtgaaag cactaatagc ttgataaata aagctcagaa tacaatagct gaaactcttg
     4981 aagccagcgc tcaggaaatc catttcactt ccggaggcac tttggctaat aacatagcta
     5041 tcaagggatt agctaatgcc tataaacagc agggaaaaca tataatcgtt tccgttgttg
     5101 attatcctga tttattaact aatgctgcct atctggaaaa gaacggtttt gaagtaactt
     5161 acctttctgc cgatagcgag ggctttattt ctctggaaga gctgaaaagt gctatccgta
     5221 aagatacaat tctttttatg actactgctg taaatcatac ggtaggaaca attcagccct
     5281 tcgcagaaat aaaagctatt ttagagagtg ccgaccataa aatttattac cacctggatg
     5341 caggtcaggg ctatggaaaa attcctttga aggtaaatga actgggaata gacacaatgt
     5401 ctatcagtgc ccataaaata cacggacctc aaggtattgg tgcgctctat caaagaacgg
     5461 gcacaaagct ggcacaattg attcatggag ttaaaagagt ggataatctg caaacaggcg
     5521 gtttaagtat tgctcttatt gccggttttg ctaaagccgt ggaacttact tttcgtgatt
     5581 ttgaagctaa tacagcttac ttaagagaac tttccaatta ccttttggac agaatagaaa
     5641 aggaaatacc ttatgttgaa ttaaacggac cccgaggaga aaaaagagct cctcacaata
     5701 tcaatgtttc cattgacttc attgaaggtg aagcaattac gatgatgctt gatcaaaaag
     5761 gaattactgt tgccacggga agtgcctgcg cttcggaagg attaaaggca aattatgttt
     5821 taatggcaat gggaaaaaca catattcagt cacacggttc aatgaaattt accgtttctc
     5881 gttatacaac taaagccgaa ctggatgaaa ccgttcaggc attagcagaa ataacacaag
     5941 aattgcgcaa tcgcagtccc ctctatttag caatgaccaa ggagaaataa aatgcaatac
     6001 tcacaaaagg ttttagacca ctttatgcat ccccataatg taggtaaaat ggaaaatcct
     6061 gatgctgtag ccacagaagg aagtcctgcc tgtggagatc aggtaaccgt ctatctgaag
     6121 gttgatgaca aaacaaagac cattacagat atcagctttc tttcttatgg ctgcgcttcc
     6181 aatattgcta ctgcttccat aattaccgat atggcaaaag gaaagacatt ggaagaagca
     6241 aaaaaaatta cctggcggga tgctatggat gctttggatg gacttcctcc cgttaaagtt
     6301 cactgcagtg ttttagctgc tgatacttta caatcggcaa tttccaa
//
LOCUS       DAOPFL010000088         6297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_16131_length_6297_cov_98.565524, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000088 DAOPFL010000000
VERSION     DAOPFL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16131_length_6297_cov_98.565524"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1143)
                     /locus_tag="PLG69_06155"
     CDS             complement(289..1143)
                     /locus_tag="PLG69_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43128.1"
                     /translation="MKKCRQRAPFHPHNKRSLNLQSCNELQLHRICSYSKLPLLLVFV
                     IILFCPTLFAQADSLQTTNFSGSGELTPLPDTLDKAIRLAEQKKNPDLFYNLGVEFFS
                     RGEIGMANLYFLKALNINSAHSPARTNLELSIHYSPDADLYPEQLFLVQVLFKVLNYL
                     SVNRLAILGLVFLFLSAVSLCWLLFYNPEKERALPILTLTFCLLLCVSAFVTLGIKLH
                     HQQHNRSAVVIVRETELLSPANNNVIQTVHSGLIVKIVQDNGNYYVVRLPNNQLGRLN
                     KEAVRKVI"
     gene            complement(1140..2921)
                     /locus_tag="PLG69_06160"
     CDS             complement(1140..2921)
                     /locus_tag="PLG69_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPI43129.1"
                     /translation="MKKALLLILLFPLVLSALKVDLSVNKNTINLNEQLELILKISDD
                     NRIKVQEPTPPSIPLFSFRNMTSSSSSSVVLEGTKLISEFSETYRFIYFPLKTGTTTI
                     PSFQVKVNGKYYATRPISIEVVKAEQKTPGQSSPPVPGIIPFGFDDSFFGGNREGWAG
                     DTFILALPKVQKVYRGFPAIVSYYLYTDEMVGSFNLENEIDSDGYGKSTYEQPTMLNY
                     EEVTINGKQYKRALIKRLALIPNREGRLQAPILEGYARLYGFGLLNKNLQSEGGIIDV
                     LPLPTNNVPPSFGGAVGNFKLSHSLSRKELSLGETITFTLKIEGRGNFNQFSAPQFAS
                     GKGFQVSTPMVIDNLNAGIEGTRTYYYTLIPQDKGVYELPELNFAWFDNDSGSYKIYR
                     SPKEQIKVSSANVLSYFSRLRQSRNPKAMLPKISRESYPDYVPYVKQPWYWILVVLIV
                     LATIVVSFVAVERKMKRDNPERYASLKAEKVLLHYLKPATAAAKNLSLDFYPLAEKAL
                     FQYLASKYKLSNHLSTQEKLQALSEKNIPEWLLDEIQLFLSHSQAARFMPETDRAVNL
                     EEDLARIRKIFTGFSRLSKNRKNRSGK"
     gene            complement(2924..3508)
                     /locus_tag="PLG69_06165"
     CDS             complement(2924..3508)
                     /locus_tag="PLG69_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI43130.1"
                     /translation="MSTKTRRNIIIIIVVILALLILELIFRPKVVRQAFAEPFYRAKK
                     YPQAENLFRKNSTKNDAPANANLAKCLYRQNRFAEADSAATIALNSAKDKKDILYDRG
                     NIAYKQQDYSKALRDYKEALLLNPEDDDLRANYELTLRKMNKQPPPAVGLQQNKDDKK
                     KEAIRNILEGLDNKESTDRQQLRSKQSLKTDKWW"
     gene            complement(3498..4493)
                     /locus_tag="PLG69_06170"
     CDS             complement(3498..4493)
                     /locus_tag="PLG69_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPI43131.1"
                     /translation="MNIANPSYLALLALCLVFLLLLFGRERKLKRRFAQYAELHLREH
                     YYHSHSPFWTGFKLFLSILALGCIIIALARPQWDYENKELQSSGMDIIFAIDVSKSMD
                     ATDMMPSRLLRAILQIGSFLEQVKNDRIGIIAFAGTATLQCPLTDDYEAVRIVLNGLN
                     SNTVEISGTDIGSALRLAENAFPEGSKSKTLVLISDGEDLRHSALREARILKTKGIRV
                     YTMGVGSPEGTIIRHPETGEEVKSKLDEATLQEIARITDGEYYRVTPGGEEIQLILKR
                     IYESESTRRGKKVTILKEQYYLFGILAIILLMIESFIDPRKRKRGIMAAEKSNEH"
     gene            complement(4490..5491)
                     /locus_tag="PLG69_06175"
     CDS             complement(4490..5491)
                     /locus_tag="PLG69_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPI43132.1"
                     /translation="MLKFGHPWLLLLLLVIPLYLIWEFVFQKKRRLYLPHSRVKQLRY
                     LSHSQYPYHFLYPVLRSCILLFLCIAVAQPRWGIKTRDLSNKGVDIVMAIDISGSMLA
                     MDFAPKNRLSAAVSVAKDFVKRRPNDRFGLVAFSEYALTQVPLTFDHLAMLNSLDKLK
                     VNEEASATAIGMGLAKAVARLKNSTAKSKVIILITDGVSNTGEIDPLTAAEMAKELGI
                     KVYPIGVGSKGLVPFPYSDPIFGTRYINTYIDLDMETLNKIAATTGTGKAALATDAKG
                     LADIMNEIDRLEKTLFTTQFRYNYSEQFMPFLWLAFAFLVLELLLKIFILPVLPEQL"
     gene            complement(5484..>6297)
                     /locus_tag="PLG69_06180"
     CDS             complement(5484..>6297)
                     /locus_tag="PLG69_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43133.1"
                     /translation="LQGADSLKVGTPFLFKITTDFAIKGVSIPDTLEDFAILESKRDE
                     KNPRCWIIKIVPLNTGALSFPRIEIQPVDSSVTPVYTDGFRIYVLSVLAEGDTLLRDI
                     KPLKKYSLQPPFWVYLLLILLALAGGIYLWLKRPEKPKREYVAQPVIREPVLPAWEKA
                     LKALDELIAENLPAKGETVLYHFRLSNILRAFLEETYRFPALEMTGREIAVSLNRRNI
                     PKAQEIRKFINYCDLVKFAKREPSAGEIEENTLWLRNYLLSFSEQTRETENA"
BASE COUNT         1932 a         1300 c         1152 g         1913 t
ORIGIN      
        1 tgttatttgg ttaggtaaat tctgcttcac cataaataga ggacattcat tttccagaat
       61 tctgtgaatt ctttagcaaa gaaagaggtt gccaacccat ttttcctcaa aggcattatt
      121 cagaataaaa taactatatc ttaaacatag aaaaggagat aactcgttct gccaagtagc
      181 attactgaag cattgcttac ccattgctaa tgccatccgt aatgggtaag caatgggtaa
      241 gcaatgctat taaggaagaa attatggctg tatatctaac tgcttatttc agataacttt
      301 ccttacagct tccttattca ggcgtcccaa ttggttattg ggcaggcgca ctacataata
      361 gtttccgtta tcttgaacaa ttttcactat caatcccgaa tggacggtct gaataacatt
      421 attatttgct ggagatagaa gttccgtttc gcgcacaata actacagcac tacggttatg
      481 ttgttggtga tgaagtttta tacccaaggt tacaaacgcg gaaacacaaa gcaataaaca
      541 aaaagttagg gttaaaatag gcaaagctct ttccttttcc ggattataaa aaagcaacca
      601 gcatagcgat acagcagaaa gaaataaaaa aacaagaccc agtatagcca aacgatttac
      661 ggaaaggtaa tttagcactt taaacagcac ttgcaccaaa aacagctgtt caggatacaa
      721 atctgcatcc ggactataat gaatggaaag ttcaagattg gttcttgctg gtgaatgggc
      781 agaatttata tttaatgctt tcaaaaagta taaattagcc atcccgattt caccccgcga
      841 gaaaaattct acccctaaat tataaaacaa atcgggattc tttttttgtt ctgcaaggcg
      901 aattgcctta tccaaagtat ccggcaaagg agtaagctct cctgaaccgc taaagtttgt
      961 tgtttgtaaa gagtcagctt gagcaaaaag ggttgggcaa aacaggataa ttacaaaaac
     1021 taagagtagt gggagcttgc tgtagctaca aattctgtgc agttggagct cattgcagct
     1081 ctgtaaattg agagaccttt tattatgggg atggaacgga gccctctgtc tacacttttt
     1141 catttgccac tcctgttttt tctattttta cttaagcgac tgaatcctgt aaagattttg
     1201 cgaatacgag ccaggtcttc ttccaaattt accgctctgt ctgtttccgg cataaatctt
     1261 gctgcctgac tgtgagacag gaaaagttga atttcatcca gcaaccattc ggggatattt
     1321 ttttcactta atgcttgtag tttttcctgg gtagaaagat gattggaaag tttatatttg
     1381 gaagcaagat attgaaacaa tgccttttcc gccaggggat agaaatccag agaaagattt
     1441 ttggcagctg cggttgccgg tttcaaataa tgcaggagga ctttttccgc ttttaacgaa
     1501 gcatagcgtt cgggattatc ccttttcatt tttctctcca ctgccacaaa ggaaacaact
     1561 atggtcgcta aaacaatcaa aaccaccaaa atccaatacc agggctgttt tacataagga
     1621 acataatccg ggtagctttc cctgcttatt ttaggcaaca ttgctttagg attgcgggat
     1681 tgtcttaaac ggctgaaata acttaacaca tttgccgaag aaacctttat ttgttctttg
     1741 ggactgcggt atattttata gctgccgctg tcgttatcaa accaggcaaa attgagttcg
     1801 ggaagctcat aaaccccttt atcctgcgga attaaagtgt aataataagt tctggttcct
     1861 tcaattccgg cattcaaatt atcaataacc attggagtgg aaacctgaaa accttttccg
     1921 gaagcaaatt gaggagcgga aaactggtta aaattacccc tcccttcaat tttcagggta
     1981 aaagtaattg tttctcccaa ggaaagttcc ttcctggaaa gagaatgact tagcttaaaa
     2041 tttcctaccg cacctccaaa agaaggtggc acattattcg tgggtaaagg caaaacatca
     2101 atgatgcctc cttcgctttg gagattttta tttaaaagtc caaaaccgta aagacgggca
     2161 taaccctcta aaatcggtgc ctgtaaacgt ccttctctat tgggaataag ggctaatctt
     2221 ttaatcagag ctcttttata ctgttttcca ttgatagtta cttcttcata atttaacatc
     2281 gttggctgtt cataagtgct ttttccatag ccatccgaat caatttcatt ttccaggttg
     2341 aaagaaccaa ccatttcatc cgtataaaga taataggaaa caatagcggg aaaaccacga
     2401 tagaccttct gcactttagg taaagccaaa ataaaagtat cccctgccca tccttctcta
     2461 ttacccccaa aaaaagaatc gtcaaagcca aaaggtataa ttcccggtac cggaggtgaa
     2521 ctttgtccgg gagttttttg ttctgcttta acaacttcaa tagaaatggg acgcgtagca
     2581 taatactttc catttacttt cacctgaaag gaaggaatgg tagttgttcc cgtcttcaaa
     2641 ggaaaataga tgaaacgata ggtctccgaa aactcggaaa ttaattttgt cccttccaaa
     2701 acaaccgaac tgcttgagga actggtcata ttacgaaaac tgaataaagg aatagaaggt
     2761 ggagttggtt cttgaacttt aatccggttg tcgtcagaaa tctttaaaat caattccagt
     2821 tgttcattta aattgatagt atttttattt acggataaat ccactttcaa agcagaaagc
     2881 acaaggggaa acagcaaaat cagaagaagt gcctttttca tttttaccac catttatccg
     2941 tttttaagct ttgtttagag cgcagttgct gtctatccgt actttcttta ttatccagtc
     3001 cttccagaat attacggatt gcttcctttt tcttatcgtc cttattttgt tgtaatccaa
     3061 ccgctggagg gggttgttta ttcattttgc gtaaagtaag ctcataattt gctcgtaaat
     3121 catcatcttc aggatttaat aatagtgctt ctttataatc cctcagagct ttactataat
     3181 cctgttgttt ataggcaata ttgccgcgat cgtaaagaat atccttttta tctttggcgc
     3241 tatttaaggc aattgtggca gctgaatccg cttcagcaaa acggttttgc cgatataaac
     3301 attttgccag attggcatta gcgggagcat cgtttttagt agaattttta cggaataggt
     3361 tttctgcttg gggatatttt tttgccctgt aaaatggctc tgcaaatgct tgacgcacta
     3421 ccttgggtcg gaaaatcaat tccagaatta gcaaagccaa aataaccact ataattatta
     3481 tgatgttacg tcttgtttta gtgctcattg cttttctccg cagccataat gccgcgtttt
     3541 cgtttacggg ggtctataaa actttctatc attaataaga taatcgccaa aatgccaaag
     3601 aggtaatatt gttcctttaa aatggttact ttctttcctc tgcgagtgct ttcgctttca
     3661 tagatgcgtt tcaaaatcag ctgaatttct tctccgccag gagttacacg ataatattca
     3721 ccatccgtta tccgggcaat ttcttgaagg gttgcctcat ccagtttact tttcacttct
     3781 tcaccggttt ccggatggcg aataattgtc ccttcagggc taccaacacc catagtatag
     3841 acccggatgc cttttgtttt taagattctt gcttctctga gagcagaatg ccgaaggtct
     3901 tccccatccg aaatcaaaac gagggttttg gattttgagc cctcgggaaa ggcattttct
     3961 gctaaacgca aagcacttcc aatatcggtt ccggaaattt ctaccgtatt actgttcaaa
     4021 ccatttagca caattctaac tgcttcataa tcatctgtta agggacattg caaagttgcc
     4081 gttccggcaa aagcaataat tccaatccgg tcgtttttaa cctgctctaa aaaactgcca
     4141 atctgtaaaa tagcacgcaa caatctactg ggcatcatat ccgttgcatc catactttta
     4201 gaaacatcaa tggcaaaaat aatatccatt ccactacttt gtaattcctt gttttcataa
     4261 tcccattgag gtcttgccag cgctattatg atacaaccca aagccaaaat agaaagaaaa
     4321 agcttgaacc ccgtccaaaa aggagaatgg gaatgataat aatgctccct taaatgtaat
     4381 tcggcatatt gggcaaaacg ccgctttaat tttctttccc ttccgaatag caatagcaga
     4441 aataccaagc ataacgctaa aagagccaga taagaaggat tggcaatatt cataactgtt
     4501 ccggcaacac aggtaaaata aatattttta acagcagttc aagcactaaa aaggcaaaag
     4561 ccagccacaa aaatggcata aactgctcac tgtaattata gcgaaattga gttgtaaaaa
     4621 gggtcttttc cagacggtca atttcattca taatatctgc aagacctttg gcatcggttg
     4681 ctaaagccgc ttttccggtt cctgtggtcg ctgctatttt attcagggtc tccatatcaa
     4741 gatcaatata ggtattaata taacgcgtgc cgaaaatagg gtcagaataa ggaaacggaa
     4801 ctaaaccttt acttcccact ccaataggat aaacttttat tcccaattcc ttagccattt
     4861 cagccgcggt taaagggtct atttctccgg tattgctaac tccatcggta atcaaaataa
     4921 tcactttgct tttggcagtg ctatttttca aacgggctac cgcttttgcc aaacccattc
     4981 cgatagctgt tgccgatgct tcttcattca cttttaactt atccagggaa ttcagcattg
     5041 ccaaatggtc aaaagttagc ggaacctgcg ttaaagcata ttccgaaaaa gccactaagc
     5101 caaatctgtc attgggacgc cttttcacaa aatccttggc tacacttaca gctgcactta
     5161 aacgattttt aggagcaaaa tccattgcta acattgaacc actgatatct atagccatta
     5221 caatatccac acctttatta cttaaatccc tggtttttat tccccaacgc ggttgcgcta
     5281 ccgcaataca taaaaataac aagatgcaag aacgcaaaac aggatacaaa aaatggtaag
     5341 gatattgact gtgtgataga tagcgtagct gttttacccg agaatgaggt aaataaagac
     5401 gccgtttctt ttgaaagaca aattcccaaa taaggtataa tggtattacc aataaaagta
     5461 aaagcaacca gggatgaccg aatttaagca ttttccgttt cccgagtttg ttcactgaaa
     5521 cttagcagat agttcctcaa ccataatgtg ttttcttcaa tttcaccggc agaaggttca
     5581 cgcttggcaa acttaaccaa atcacaataa ttaataaact tccttatttc ctgtgctttg
     5641 ggaatatttc tccggtttaa actaactgca atttcccttc ctgtcatttc cagagcggga
     5701 aaacgatagg tctcttccaa aaaagcacgc aaaatattag ataggcggaa atggtataaa
     5761 accgtttctc ctttcgcagg caaattctct gctataagtt catccaatgc ttttaatgcc
     5821 ttttcccagg caggtaaaac cggttctcta atcaccggtt gagcaacata ttcccgtttg
     5881 ggtttttccg gtcgcttcaa ccaaagataa attccccctg ccaaagcaag caaaatcagc
     5941 aatagataaa cccagaatgg cggttgaagg gaatactttt tcaggggctt aatatcccgt
     6001 aaaagagtat ctccttccgc taaaacagaa agcacataaa ttctgaagcc atctgtgtaa
     6061 acaggagtta cactactgtc aacaggttgt atttctatcc ggggaaaaga taaagcacct
     6121 gtatttaggg gaactatttt tattatccag caacggggat ttttttcatc ccgtttactt
     6181 tccagaatag caaaatcctc taatgtatcg ggtatggaaa caccttttat ggcaaaatcg
     6241 gtagtaattt taaagagaaa aggagtgcct acttttaaac tatcggcacc ctgaagt
//
LOCUS       DAOPFL010000089         6140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_16736_length_6140_cov_102.286771, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000089 DAOPFL010000000
VERSION     DAOPFL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16736_length_6140_cov_102.286771"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLG69_06185"
     CDS             <1..333
                     /locus_tag="PLG69_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPI43134.1"
                     /translation="TLLGNRYLTPQSTVFIDEPEAALHPSAVSLFLEIISDLAKLGMQ
                     FFMATHSYFVIKKLYLIAQEKKLNIPIASYEDDKWVYDDLRNGMPDNSIVNETIKLYK
                     EEVDLVLK"
     gene            330..842
                     /locus_tag="PLG69_06190"
     CDS             330..842
                     /locus_tag="PLG69_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43135.1"
                     /translation="MNKIVIEESNMKFGPYDKEAVFLIEQSRVYKEIGNNVKIAEFVL
                     EKSSQKINIFEVKSSSPQPKNSEDYDNFIRSIYEKFVNTFSLTLALILKRFPSHYEVL
                     PERLEKIEVSNFKCLFVLVIYGHKEEWLLPIQDSLNKSLSSFFKIWNLPPTSVIVINE
                     KIAKAKHIIK"
     gene            851..1876
                     /locus_tag="PLG69_06195"
     CDS             851..1876
                     /locus_tag="PLG69_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HPI43136.1"
                     /translation="MNTDYLNLAKAFLYKQRLWQTCGIVCEYGLSLITHKPLVHFYPP
                     ALMLEPTNICNLHCPLCLTGNGKLLRPRGMMSLPLFKKICREVQNKIGMLILWSQGEP
                     FLNPDFYAMLEYASKQNFYTMTSTNASLELDIERIVKSGLHKIIISMDGITAETYNKY
                     RINGNFDLVLKNMQELVHQKEKLHSNKPYIVWQFLIMKHNENEIEEVKHLARKLKIDK
                     LEFKTAQIYSSDDLAFLPSDHKYSRYFQQGNKFTLKTKLKNRCIRLWTQPVINWDGEM
                     NICCYDKNGSIKIGNIQEHSFSELWCGEKMQKMRKAILNNRSAFEICQNCGEGIVQKI
                     KMEKGER"
     assembly_gap    1879..1978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2051..3136
                     /locus_tag="PLG69_06200"
     CDS             2051..3136
                     /locus_tag="PLG69_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPI43137.1"
                     /translation="MKISEIMKQIEEFAPLGLALSWDNSGLQIGDLNSEVENVYITLD
                     VTSTAVQKALENECQLILSHHPLIFQQLKNITNPLYLEIIKNNLSVISLHTNLDVAEK
                     SVNHSLAQKLGLEVIGFLSSETGATNYIIVVYCPLEDAEKIMHSAWSAGAGKIGNYYN
                     CANKQESEGYYKASENAKPYKDSGIEGHIAETCLQFCCESFHLNDVLNAIRQNHPYET
                     PLIYHYEVANPNPAYGLGLICKYNAEINLQDLAILVKERLNCPQVKLWTASFDLHKNI
                     NRIAICGGSGSSLISAAQNSADVLITGDISYHSFLESKIPLIDAGHFYTEYPALQLLA
                     DFVNKLQLHPFLIPQNEHLYTQNLLII"
     gene            3167..4234
                     /gene="ispG"
                     /locus_tag="PLG69_06205"
                     /gene_synonym="gcpE"
     CDS             3167..4234
                     /gene="ispG"
                     /locus_tag="PLG69_06205"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPI43138.1"
                     /translation="MQEIKRKNTRRIQLGNIPIGGGAPVSIQSMLSVKTSDRENAIQQ
                     IQELESVGCDIIRCAVMDLDDAKAIKDLKKIAQAPLVADIHFNYRLALAAMENGIDGL
                     RINPGNIGKIEGVKALVASAKERKVPIRIGVNSGSLPKDLLQKYGVSAKAMVESALRH
                     IRILEELSYYEIKVSVKASSIPLTLESYRLLSSVCDYPLHLGITEAGTVFRGSIKSSI
                     ALGILLDEGIGDTIRVSLTDKPVQEVKVAKEILTSLGLRKGLNIISCPTCGRTRIDLI
                     KLANEVEKALEPYNNYPLTIAVMGCAVNGPGEAKEADFGIAGGIKEGLLFAEGKVLKK
                     VPEAFLVNELVALVQDYISKH"
     gene            4237..4770
                     /locus_tag="PLG69_06210"
     CDS             4237..4770
                     /locus_tag="PLG69_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPI43139.1"
                     /translation="MLLKIFFTFFKIGAFTIGGAYAMIPLIKREVCQKNNWLTEDEFW
                     DGLSAAQSCPGPIAINLSIYIGYHLAKGKGMAIAVLATILPSFIIILIIAMLFQQFAE
                     ITLIQKAFHSLRPAVVALIAVPLIQMSRQSGLALSNFWFPLSVAILVGFFRISPVYLI
                     LITIAFAVYQGLKKQKL"
     gene            4767..5285
                     /locus_tag="PLG69_06215"
     CDS             4767..5285
                     /locus_tag="PLG69_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPI43140.1"
                     /translation="MIYFQLFLTFFKIGLFSFGGGYAILAMINQEVVLKNAWITHSQF
                     MDIVAISQMTPGPIAINAATFIGYQKGGFVGSVLSTLGVISPSLIIMTFITLTYIKLK
                     NQPWFKNIFQKLRLLSLGLIAAAFIMVAGDAFRDLFSVLVFTASFIASWKFKLNPFYL
                     LIGAAIVGIAFG"
     gene            complement(5400..>6140)
                     /locus_tag="PLG69_06220"
     CDS             complement(5400..>6140)
                     /locus_tag="PLG69_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPI43141.1"
                     /translation="LGIIHLINQIRDGKTPYPQSFFFAGKAAPGYFIAKLIIRFICAL
                     SDYIEKDKELSRYLSVIFLPNYRVTLAERIMPPAEISRQISLAGTEASGTGNMKFALN
                     GALTIGTLDGANIEIREAVGEENFFLFGMTAAEVKNLKESGYHPYEMMRQNEDLKRIF
                     DFIESDILNPLNPGLFTPLTDLLLYQGDKYCLIADLPDFIRVNTEAVKVYQDKQQWNR
                     MSLANIANMGKFSSDETIKGYAKDIWGV"
BASE COUNT         1963 a         1017 c         1140 g         1920 t
ORIGIN      
        1 actttattgg ggaatagata cttaacccct caatcaacag tttttattga tgaaccagaa
       61 gctgccttac acccttccgc agtttctcta tttttagaaa taataagtga tttagcgaaa
      121 ctgggaatgc agttttttat ggcaacacat tcatattttg ttattaaaaa actctatcta
      181 atcgctcagg aaaagaaact aaacatacct attgcttctt atgaagatga taaatgggtt
      241 tatgatgatt tgaggaatgg aatgcctgat aattcaatag tgaatgagac cattaaatta
      301 tataaggaag aagttgatct ggtgctgaaa tgaataaaat agtaattgaa gaatcaaata
      361 tgaaatttgg accttatgat aaagaagccg tttttctgat agagcaaagc agggtatata
      421 aagagatagg taataatgta aaaattgccg aatttgtttt ggagaaatca tcccagaaaa
      481 ttaatatttt tgaagtaaag tcaagttctc cccaaccgaa gaatagtgaa gattatgata
      541 actttatcag aagcatttat gaaaaatttg ttaatacttt ttctcttacc ttagctttaa
      601 ttttaaaaag gttcccttcc cattatgaag ttctgccaga aaggttagaa aaaatagagg
      661 tatcaaactt taaatgctta tttgttttag taatttatgg acacaaagaa gaatggttac
      721 ttcccataca ggattcctta aataaatctc tatcttcatt ttttaaaata tggaaccttc
      781 ctccaacttc ggtaatagtc ataaatgaga aaatagccaa agctaagcat attatcaaat
      841 agctattttt atgaatacgg attatcttaa cttggcaaag gcatttttgt ataaacagcg
      901 tttatggcaa acctgtggaa ttgtttgtga atacggttta tcactgataa cccataaacc
      961 gctggtgcat ttttatccac ctgccttaat gctggaacca acaaatattt gtaatttaca
     1021 ctgtccttta tgtttaaccg gcaatggaaa attgcttcgt cccaggggaa tgatgtcttt
     1081 accgctattt aaaaaaatct gtagggaagt tcaaaataaa attggaatgc tgattttatg
     1141 gagtcaaggc gaaccctttc tgaatcccga tttttatgca atgctggaat atgcctctaa
     1201 acaaaatttt tacactatga cctcaactaa tgccagcttg gaattagata ttgaacgcat
     1261 tgtgaaaagc ggtttgcata aaattattat ttcaatggat ggaattacag cagagaccta
     1321 taataaatat cggataaacg gaaactttga tctcgtgctg aaaaatatgc aggaactggt
     1381 tcaccagaaa gaaaaattgc atagcaacaa gccatatatc gtttggcagt ttttgattat
     1441 gaaacataac gaaaatgaga ttgaagaagt taaacacttg gcgagaaaac tgaaaattga
     1501 taaactggaa ttcaaaacgg ctcaaatata cagttctgat gaccttgctt ttttaccttc
     1561 cgatcataaa tacagtcgct atttccaaca gggcaataaa tttactctga aaaccaaact
     1621 gaaaaatcgc tgcatacgtt tatggacaca accggttatc aattgggacg gggaaatgaa
     1681 tatctgctgc tacgataaaa atggtagcat aaaaatcggc aatattcaag agcacagttt
     1741 ttctgaactt tggtgtggag aaaagatgca aaaaatgcgg aaggcaattt taaataatcg
     1801 ttccgctttt gaaatctgcc aaaattgcgg tgaaggaatt gtgcaaaaaa tcaaaatgga
     1861 gaaaggtgaa aggtgaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
     1981 aacagatact gcttgacggg ataagcattt tattttacct gtttttgcat cagtaagatg
     2041 aggtatcatt atgaaaataa gtgagataat gaaacagata gaagaatttg ctcctttggg
     2101 attagctctt tcctgggata attctggttt acagataggt gacttaaaca gtgaagtgga
     2161 aaatgtttac ataactttgg atgtaacctc aacagctgtt caaaaagcat tagaaaacga
     2221 atgtcaattg attttaagcc atcatccttt aattttccaa cagcttaaaa atataacaaa
     2281 tcctctttat ttggaaatca tcaaaaataa cctttccgta atttccctac ataccaacct
     2341 ggatgttgcg gaaaaaagtg taaatcatag tttagcccaa aaactcggtt tggaagttat
     2401 tggtttttta agttctgaaa ctggagcaac taactatatt atagtggttt attgccctct
     2461 tgaagatgcg gaaaaaataa tgcattcagc ttggagtgca ggtgccggaa aaataggaaa
     2521 ctattacaat tgtgccaata aacaggaatc cgaaggttat tacaaagctt cggaaaatgc
     2581 caaaccctat aaagatagcg gtattgaagg tcatattgcg gaaacctgtc ttcaattctg
     2641 ctgtgaaagc tttcatttaa atgatgtctt aaatgcaatt cgtcaaaatc atccttatga
     2701 aacaccgttg atttatcatt atgaggttgc taatcccaat ccggcttatg gattgggttt
     2761 gatttgcaaa tacaacgctg aaataaatct tcaggacttg gctattttag ttaaagaacg
     2821 cttaaattgt cctcaggtta aactttggac agccagtttt gacctgcata aaaatattaa
     2881 caggatagct atttgtggag gtagtggcag ttctctgatt tcagccgctc aaaatagtgc
     2941 cgatgtttta attaccggag atatttccta tcacagcttt ctggaaagca aaattcctct
     3001 tattgatgcg gggcattttt atacagaata tccagctttg cagttacttg cggattttgt
     3061 taacaagttg caactgcacc cttttttaat ccctcaaaat gaacatttat atacccaaaa
     3121 ccttctaatt atttaaaata gcagtaacaa acaaggtgag aacaagatgc aggaaatcaa
     3181 gcgtaaaaat acaaggcgta ttcaattagg aaatatccct ataggtggag gtgcacctgt
     3241 aagcattcaa tcaatgcttt cagtgaaaac ctccgaccgg gaaaatgcca ttcaacaaat
     3301 tcaggaactg gaatccgttg gttgtgacat aatccgttgt gcagtaatgg atttagatga
     3361 tgccaaggca attaaagacc tgaaaaaaat tgcccaagct cctttagtag cagatattca
     3421 ttttaattac cgtttagccc ttgcagctat ggaaaatggt attgacggac tgcgtatcaa
     3481 tcctggaaat ataggaaaaa ttgaaggtgt gaaagcattg gttgcttcag caaaagaacg
     3541 aaaagttcct attcgcattg gagtaaatag tggttctcta cctaaggatt tactgcaaaa
     3601 atatggagtt agcgctaagg caatggtgga atcggcttta cggcatataa gaattttgga
     3661 agagctttct tactatgaga ttaaggtttc cgtgaaggca tcctcaattc ctttaacatt
     3721 ggaaagttac cgccttttaa gcagtgtttg tgattatccg ttacatcttg gaattaccga
     3781 agcaggaact gtttttcggg gttccataaa aagttccatt gctttaggaa tccttttaga
     3841 tgaagggatt ggagatacga taagggtttc tttaacggat aagccggttc aggaagtgaa
     3901 agttgccaaa gaaatattga cctctttggg tttacgcaaa ggattaaata ttatatcctg
     3961 tcctacctgc ggacgaacaa gaatagacct gattaaatta gctaacgaag tggaaaaagc
     4021 acttgagccc tataacaact atcctttaac tattgctgtt atgggttgtg ctgtaaacgg
     4081 acccggagaa gcaaaagagg cagattttgg cattgcagga ggcatcaaag aaggattgct
     4141 ttttgcagaa ggcaaggtct tgaaaaaagt tccagaggca tttttagtaa atgaacttgt
     4201 ggctttagtg caggattata tcagtaaaca ttgaagatgc ttcttaaaat attcttcact
     4261 ttcttcaaaa taggcgcttt taccattggc ggtgcttatg caatgattcc tttaattaaa
     4321 agagaggtct gccaaaaaaa taactggctt accgaagatg aattttggga tggactttct
     4381 gcggcacaaa gctgtccggg acctatagcc atcaacttaa gtatttatat tggctaccat
     4441 ctggcaaaag gtaaaggaat ggcaattgct gttttagcta caattctgcc gtcttttatc
     4501 attattctta ttattgctat gctgtttcaa cagtttgccg aaattacttt aattcaaaag
     4561 gcatttcatt ccttgcgtcc tgcagttgta gctttaatag cggttccctt aattcaaatg
     4621 agcagacaaa gcggattagc tctatccaat ttttggtttc ctcttagcgt tgctatttta
     4681 gttggttttt tcaggataag tcctgtttat ttgattctaa tcactattgc ctttgctgtt
     4741 tatcagggat taaaaaagca aaaattatga tttattttca gctgttcctg acttttttca
     4801 agattgggct tttcagtttt ggtggcggat atgccatttt ggcaatgata aatcaagaag
     4861 tagtattaaa aaatgcctgg attacccatt cccagtttat ggatattgta gctatttcac
     4921 aaatgacacc gggacctata gctatcaatg cagctacttt catcggttat caaaaaggag
     4981 gttttgtagg ttctgttctt tctactttag gagttatttc cccttcgctg attattatga
     5041 ctttcataac gttaacctat atcaagctta aaaatcaacc ctggttcaag aatatttttc
     5101 agaaactgcg tttgttatcc ttgggattaa ttgccgccgc ttttataatg gttgcaggag
     5161 atgcttttcg tgatcttttc tccgttttgg tatttactgc atcttttata gcgagctgga
     5221 aatttaagct caatcctttc tatttgctta tcggagcagc tattgtaggc atagcattcg
     5281 ggtaaaaagg aagaatgaat atacttctat agtaacgaat atattataaa tccctttatt
     5341 tatgttcaat tatacctagt gtcaaataaa atccgcgaaa tccgcgtgct attttcattt
     5401 caaactcccc aaatatcttt tgcatatcct tttatcgttt catcggaaga aaattttccc
     5461 atattggcaa tattggctaa agacattcta ttccattgtt gtttatcttg atagaccttt
     5521 acggcttcag tattaactct tataaaatca ggcagatcag caattagaca atatttatct
     5581 ccctgatata gaagcaaatc cgttaaggga gtaaaaagtc cgggatttaa aggattcagt
     5641 atatcggact caataaaatc aaaaatgcgt ttaagatctt cattttgacg catcatttcg
     5701 taagggtggt aaccgctttc ttttaaattt ttcacttcgg cagcggtcat tccgaagagg
     5761 aagaaatttt cttctccgac tgcttcccga atttctatat tggcaccatc caaagttcca
     5821 atagtaagag ctccattgag agcaaatttc atatttcctg ttccggaagc ttcagttcca
     5881 gcaagagaaa tttgacggga aatttctgcc gggggcataa ttctttctgc caaagttacg
     5941 cgataattgg gtaaaaagat aacacttaag taacgggaaa gctctttatc cttttctata
     6001 taatcactta aggcacaaat aaaacgaata atcaatttgg caataaagta gccaggagcc
     6061 gcttttcccg caaagaagaa actttgagga taaggggttt ttccatcccg aatttgattt
     6121 atgagatgga taatacccaa
//
LOCUS       DAOPFL010000090         6048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_17127_length_6048_cov_109.380277, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000090 DAOPFL010000000
VERSION     DAOPFL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17127_length_6048_cov_109.380277"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..1092)
                     /locus_tag="PLG69_06225"
     CDS             complement(301..1092)
                     /locus_tag="PLG69_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPI43142.1"
                     /translation="MIYSDIYLGDNKFPLLAFAIHNGNYMPPELLQNCGIDADTRLRE
                     EDPYTNGFAECFPNRIIVQTSRFAVDLNRPPEKAVYQKPEDAWGLQVRKMPIADNLYT
                     KLLKAYSDWYQIIRYQIERLLKIHPTLIILDLHSYNHRRGGPDVAPDPQNQNPDLILG
                     RNNLPESVYPLVENLRLLLDGNPFQNIKLDCRCDIKFSGGHFSRWVNQTFGNKVLCLA
                     IEFKKIFMDEWTGELNLPAYFQLKEIFQTTVLKWMSEITSLEKKG"
     gene            complement(1089..1748)
                     /locus_tag="PLG69_06230"
     CDS             complement(1089..1748)
                     /locus_tag="PLG69_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43143.1"
                     /translation="MTRDFSEVMNTLSGIQDYLLQVAIYDDFNPECIIILEKDPPDLL
                     ESLDSLRKYSHKRQLRFPLLVNRKFVESSLDSYPLEFLDIITSNYQNLFVKEDILKNL
                     QFDPDDLRLQMEREIKGKWLLIRLSVLERKNTPKQLSDLLFMALSNIIPVLKGICYLY
                     DGVVPLKLEEILAKNNIITNVRFEPMLDWVSGDEATLEDIKQYLGILEGLMQYLEQLD
                     Q"
     gene            complement(1763..2608)
                     /locus_tag="PLG69_06235"
     CDS             complement(1763..2608)
                     /locus_tag="PLG69_06235"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPI43144.1"
                     /translation="MNFQDLILSLQNYWAEKGCCLIQPYDLEMGAGTFHPSTFFGTLG
                     KKPCAIAYPQPCRRPKDGRYGENPNRFQHYYQFQVIIKPTPDDIQNLYLKSLSAIGID
                     ISMHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVASIDVFPVSVELTYG
                     LERIAMYIQNVDDYRLLAWNENIGYAELFYEREKEYSAYNFEEADTTLLFQLFQDYEK
                     ECNALLGKQLIYPAYDMLLKCSHTFNLLEARGVISVTERASYIARIRNLARACANAYL
                     TKYNA"
     gene            complement(2605..3000)
                     /locus_tag="PLG69_06240"
     CDS             complement(2605..3000)
                     /locus_tag="PLG69_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPI43145.1"
                     /translation="MKKYYYTIGEVSNLLGVKPYIIRYWETEFPGLNAIKSEGRIRKY
                     NEQQVLLLRRIYDLLYNQRYTIEGARKIIKQEKNKIQTKTKETLDDSLSSPKKDSEMK
                     TEITEILQDIKKDLTLIQQTCKNYNQDKK"
     gene            complement(2997..4499)
                     /gene="lnt"
                     /locus_tag="PLG69_06245"
     CDS             complement(2997..4499)
                     /gene="lnt"
                     /locus_tag="PLG69_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPI43146.1"
                     /translation="MDLLWVLLSALMLALSRLPIHCGWMVFFGWIPLLYVLERRKVKL
                     WQAGLLFSIVYIGVVMYWIAGVTFFGLIGIAIVYFLFYWLCFYAISRIGRFYPRLFYP
                     NFIAVMLSFEYLQNFGETRFPWFNNAYSLSDYTVLIQAGDLGGVILLSLLILLINVLL
                     YEVLLKKKTQCLLYVAGIFILWIGYGIYCLNYLPVQKQDPKITVMQPSIPQDEKWDES
                     AYQQILHRYDALCAQAKMDSTKLIIFPEAAMPVYLMYDYRARNYLNDLMQKYELKIFT
                     GFPHFTLAPENHINRELYYNAAALFQPDGSISELYYKNILVPVGERMLWLNIFPFLWK
                     LQFGQANWEFGNGPVYYQTDSLTFSPSICYELAFAEFHQQMAIRKDKTTGKLKKADFL
                     VNITNDAWFGTSYGPWLHSIMAKFRAVENRIQIYRSANTGISLIVDPFGRIIAKAGLF
                     EITTLQAPLYRCSRIPVYRYIYWYPWIFVILAGGLFLFSFTKKKQKGEVL"
     gene            complement(4503..5339)
                     /locus_tag="PLG69_06250"
     CDS             complement(4503..5339)
                     /locus_tag="PLG69_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43147.1"
                     /translation="MRKVLFLAIIVSLAICLVVLNCCSDINRRTPMDIEYFNLVKAIP
                     LGMQTDKIQYHSPSNTLYAMSIKSQEIYIFQDQERKNVLGGLGSSKVNFQYLADIAMG
                     SDNSLYVLDSSARIVKKFSSDGSPSGSIELKNSVQPKCLCVYQDQGLFIFDSASGEII
                     NYSGIDGTELNRFGRFQLQQVNSLFCNHDYLIAYDKANNVSNIFSVLGQFVKTEQGQV
                     VYDLYNNAICFNAGMLKCLSSSAYLPLAAENVFMCISNDLLALRIGEEIRIYKINYAR
                     VE"
     gene            complement(5344..>6048)
                     /locus_tag="PLG69_06255"
     CDS             complement(5344..>6048)
                     /locus_tag="PLG69_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPI43148.1"
                     /translation="ACDIIEKKYKDLQQTSCMVVSSDDWHPGVIGIVASKLVEKYYRP
                     VIMISFKDGFGSGSGRSVADFDLFEALKQTEHNLHSFGGHKYAVGLTIYQEYLDRFEN
                     ELTRFVSENLRLEQIQPPLQIDAEIELYNINNTLLDALEHFAPFGPDNMRPVFMTRNV
                     TIAGYPYNVGRNHLKLKVVKDGIYFDLIGYNLGDYLPLLKKNGKLNIAYTLEYNRFGN
                     NLTIQGKLKDLQILKD"
BASE COUNT         1967 a         1187 c         1076 g         1818 t
ORIGIN      
        1 cattagcaat gggtaagcaa tgggtaagca atgctattag ggaagagaat ataatctatt
       61 gtgttatggt gaattagcaa aataattagg tgtgggtctt tgtgctggaa agtttcagga
      121 aaatatcaaa agtcgtcacg agtcataagc gccaaaaaag ttatttcaaa ggtcaaagat
      181 tagaaggtgc taatgactct tgacttaaat ttttccgagg agcttacaca cccatcgcta
      241 tcattgtatc gccctgccgg ctttgaaaaa atctcaacct tataaaccct ctcaaccctc
      301 ttaacctttt ttctccaggg atgtaatttc gctcatccat ttgaggacgg tagtttggaa
      361 gatttctttc agctgaaaat aggcaggcag gttcagttct ccggtccatt catccataaa
      421 aatctttttg aattcaatag ccagacagag aactttgttg ccgaaggtct gatttaccca
      481 acgggaaaaa tgtcctccgc taaattttat atcgcagcgg cagtccaatt ttatgttttg
      541 aaaagggtta ccatccagta gcaaacgcaa attttctaca agaggataaa cactttcggg
      601 aaggttattt ctgcccaaaa taagatcggg attttggttt tgagggtcgg gagcaacatc
      661 aggaccgcct cttctatgat tatagctatg caggtctaaa ataattaggg tgggatgaat
      721 tttcagcaag cgttcaattt gatagcggat aatctgatac cagtcggagt atgccttaag
      781 caattttgta tacaggttat ccgcgatagg cattttacga acctgcaaac cccaagcatc
      841 ttccggtttt tgatagacag ctttttcggg aggacgattc aaatcaacgg caaaacggct
      901 ggtttgcaca ataattctat tgggaaaaca ttccgcaaag ccattggtat aagggtcttc
      961 ttcgcgtaat cttgtgtctg catcaattcc acagttctgt aaaagttctg gtggcatata
     1021 attgccgtta tgaatagcaa aagccaataa cgggaattta ttatccccca aatagatatc
     1081 actataaatc attgatcaag ctgttccaga tattgcatta aaccttctaa aatgcccaaa
     1141 tactgtttaa tatcttccaa tgtagcttca tcgccggaaa cccagtcaag cataggttca
     1201 aatcttacat tggtaataat gttatttttt gccaaaattt cctcaagttt aaggggaaca
     1261 acaccatcat acagataaca aatacctttc aacacgggaa taatattgct caaagccata
     1321 aacaggaggt cggacaattg tttgggagta tttttccgtt ccaaaacaga caaacggata
     1381 agcagccatt tgccttttat ttcgcgttcc atttgtaaac gcagatcatc aggatcaaat
     1441 tgaagattct ttaaaatatc ctctttaaca aaaaggtttt gataattgct ggtaatgata
     1501 tccagaaatt ccaggggata ggaatctaaa gaactttcta caaacttgcg gtttaccagt
     1561 aaaggaaaac gcaattgacg cttatgggaa tactttctta aagaatctaa agattccaga
     1621 agatcgggag ggtctttttc cagaataatt atgcattccg ggttaaaatc atcataaata
     1681 gcaacttgta aaaggtaatc ctgaataccg gataaagtat tcattacttc agaaaaatcg
     1741 cgggtcataa tgcctccttt ttttaagcgt tatatttggt taaataagca ttggcacagg
     1801 cgcgagccaa atttcgtatt ctggctatgt aagatgctct ttccgtaaca cttatcactc
     1861 ctctggcttc cagtaagtta aaagtgtgag aacattttaa caacatatca taagcaggat
     1921 aaattagttg tttacctaaa agggcattgc attccttttc ataatcctga aaaagctgga
     1981 acagcagagt tgtatctgcc tcttcaaagt tataggcact atattctttt tcgcgctcat
     2041 agaaaagttc cgcataacca atattttcat tccaggcaag caagcggtaa tcatccacat
     2101 tttgaatata cattgctatg cgctccagtc cataagttag ttctacacta acaggaaaaa
     2161 catctatgct ggcgacctgc tgaaagtatg taaattgaga aatttccatt ccatccaacc
     2221 agacctccca acccaaaccc caagctccta aagttggtga ttcccaatcg tcttccacaa
     2281 aacggatatc gtgcattgaa atatcaattc caatagcgga taagcttttt aagtataaat
     2341 tttgaatatc atccggagtt ggcttgataa tcacttgaaa ctgataataa tgttgaaaac
     2401 gattgggatt ttctccataa cgaccgtctt tgggtcttct acaaggttgc ggataagcaa
     2461 tggcacaagg tttttttcct aaagtgccaa aaaaggtgga aggatgaaaa gttcctgctc
     2521 ccatttccaa atcgtagggt tgaattaaac aacaaccctt ttcagcccaa taattttgca
     2581 gagatagtat cagatcttga aagttcattt tttatcctga ttatagtttt tacaggtttg
     2641 ctgaataagc gtcaggtctt tttttatgtc ctgcagtatt tccgttattt cagttttcat
     2701 ttctgaatcc ttttttgggg aagatagaga atcgtcaagt gtctcttttg ttttcgtttg
     2761 aattttgttt ttttcctgct taattatctt tctggcacct tcaatggtat agcgctgatt
     2821 atagagcagg tcgtaaattc tacgcagtaa aagaacctgc tgttcattgt attttctgat
     2881 gcgcccttca ctctttatgg cattcaaccc gggaaattca gtttcccaat agcgaataat
     2941 atagggctta acacctaaaa gattgctaac ttcgcctata gtgtaataat attttttcat
     3001 aagacctcac ctttttgttt ctttttagtg aagctgaaga ggaaaagtcc tcccgccaga
     3061 attacaaaaa tccacggata ccaataaata taacgataaa ctggtatacg ggaacaacga
     3121 tatagaggtg cttgcaaagt tgtgatttca aagagacccg ctttggcaat tatgcggcca
     3181 aaagggtcta caattaaaga aattccggta ttggcactac ggtaaatttg aatccggttt
     3241 tccactgccc gaaattttgc cataatggaa tggagccagg gaccgtagga agttccaaac
     3301 caggcatcat tagtaatatt caccaaaaaa tcggcttttt ttaatttacc tgttgttttg
     3361 tctttcctga ttgccatctg ctgatgaaat tcagcaaaag caagctcata acatattgaa
     3421 ggggaaaagg ttaaactatc cgtttggtaa taaaccggtc cgttgccaaa ttcccagttt
     3481 gcctgtccaa actgcaattt ccataaaaaa gggaaaatat tcagccacag cattctttca
     3541 ccaactggaa ccagaatatt tttataatac aattccgaaa ttgagccatc tggctggaaa
     3601 agagctgctg cattgtaata taattctctg ttaatatgat tttcaggagc taaagtaaaa
     3661 tgcggaaagc cggtaaaaat tttcagttca tatttttgca tcaggtcatt caaatagttt
     3721 cgtgcccgat aatcatacat taggtaaacc ggcattgcgg cttccggaaa aattattagt
     3781 ttggtggaat ccatttttgc ctgagcacaa agagcatcat aacgatgtaa tatttgctga
     3841 taggcactct catcccattt ttcgtcttga ggaattgagg gttgcataac ggttattttg
     3901 gggtcttgtt tttgaacggg caaatagttt aaacagtata taccataacc aatccaaaga
     3961 ataaagatgc cggctacata taatagacat tgcgtcttct ttttaaggag aacttcataa
     4021 agcaaaacat tgatgagcaa aataagcaaa gaaagtagaa ttacccctcc caaatcacct
     4081 gcctgaatta aaacagtata gtccgatagc gaataggcat tattaaacca cggaaatctt
     4141 gtttctccaa aattttgcaa atattcaaag cttaacatca cagcaataaa attagggtaa
     4201 aaaagacggg gataaaatct acctatcctg ctaattgcat aaaagcaaag ccagtaaaat
     4261 aagaaataga caatagcgat gccgatcaaa ccaaaaaaag taactcccgc tatccaatac
     4321 attacaacgc cgatataaac aatagaaaat agaagtcctg cctgccataa tttcactttt
     4381 cttcgctcta aaacatagag caagggaatc caaccaaaga aaaccatcca accacaatgt
     4441 attggtaaac gcgataaggc aagcattaaa gccgaaagta aaacccaaag taaatccatc
     4501 acttattcca cccgcgcata gtttatttta taaatacgga tttcttctcc tattcttaaa
     4561 gctaataaat cgttactgat acacataaat acattttccg cagctaaagg taaataggca
     4621 gaactggata aacatttcag cattcctgca ttaaaacaga tagcgttatt gtataaatca
     4681 taaacaacct gtccctgttc cgtctttaca aattgaccca gcacggaaaa tatgttactt
     4741 acattatttg ccttgtcata agcaattaga taatcgtgat tgcaaaaaag agaatttacc
     4801 tgctgaagct gaaatctgcc aaaccgattc agttcggttc cgtcaattcc agaatagttg
     4861 ataatttctc cgctggcaga atcaaaaata aataaaccct ggtcttgata tacacaaagg
     4921 catttgggtt gcacactgtt tttaagctca atactgccgg aaggactgcc gtcggaactg
     4981 aattttttca caatgcgagc agatgaatcc aacacatata aagaattatc actacccata
     5041 gcaatatctg ctaggtattg aaaattaact ttggaactgc ctaaaccacc aagcacattt
     5101 ttccgctctt gatcctggaa aatataaatt tcctgacttt taatgctcat tgcataaagc
     5161 gtattgctgg gggaatgata ctgaatttta tctgtctgca ttcccaaagg gatagcttta
     5221 accaaattaa aatattcaat atccattgga gttcttctgt tgatgtcgga acagcaattt
     5281 agcacaacca ggcagattgc taaagaaaca atgatggcta aaaataaaac cttccgcata
     5341 gttctaatcc tttagaattt gtaaatcctt aagcttgcct tgaatggtta aattattccc
     5401 aaaacggtta tattcaaggg tataggcaat attcagtttt ccattctttt tcagcagagg
     5461 tagataatct cccaaattat aaccaattaa atcaaagtaa atgccatctt taactacttt
     5521 cagtttcaga tgatttcttc ctacattata aggataacct gctatagtta cattgcgggt
     5581 cataaaaaca ggacgcatat tatcaggacc aaaaggagca aaatgctcca aagcatcaag
     5641 caaggtattg tttatgttgt aaagctcaat ttcggcatct atctgtaaag gtggttgaat
     5701 ttgttccagg cgtaagtttt cggaaacaaa cctggttaat tcgttttcaa aacggtctaa
     5761 atattcctga taaatagtta aacccacagc atatttatga cctccgaaac tatgcaaatt
     5821 atgctctgtc tgcttcaatg cttcaaaaag atcaaaatcg gcaacactgc gtccggaacc
     5881 actgccaaaa ccgtctttga aggaaatcat aatcaccgga cgataatatt tttctaccag
     5941 cttggaagca acaattccga ttactcctgg atgccagtca tcggaagaaa cgaccataca
     6001 ggatgtttgt tgtaagtcct tatatttctt ttcaataatg tcacatgc
//
LOCUS       DAOPFL010000091         6023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_17218_length_6023_cov_82.650804, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000091 DAOPFL010000000
VERSION     DAOPFL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17218_length_6023_cov_82.650804"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLG69_06260"
     CDS             <1..330
                     /locus_tag="PLG69_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43149.1"
                     /translation="NNPQQFAEYTDMNQLSSDIAKFSSSILLNNLSISMSKYLGSSIF
                     LDYMLKLQEATNLQNRTRILVSNEASIRLILPKNYRVAYTVTYEAQEKKTSHEIMLSR
                     FIRFWGL"
     gene            554..1048
                     /locus_tag="PLG69_06265"
     CDS             554..1048
                     /locus_tag="PLG69_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43150.1"
                     /translation="MPDNVKNENAVNTKKTPKMADFSTRVSLVELIMILMLVGLVFVF
                     YFGMKQLQIDKANEAIAQEKFENIIPTFKNIIEAMETYRKADEFGDYPAFLEELNLGD
                     INTNDFKFEYSADTYTITAITQPAFGKAGIKVIYNLSDKSFTVEDPIPDKKPTIKDEW
                     LPQD"
     gene            complement(1427..2356)
                     /locus_tag="PLG69_06270"
     CDS             complement(1427..2356)
                     /locus_tag="PLG69_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43151.1"
                     /translation="MSKYKEIDLKKIKTYPIDQRYSKVNINAFAKKGKVKVNDFFNCL
                     PDILAAKDLKELILTCKKARENHKPIIIGLGAHIIKCGLAPLLIEMMEEDFVSAFVCN
                     GAVAIHDYEIASFGKTSEDVSSALSDGSFGMAEETVKGINEAINKGFKESLGLGEAIG
                     KELYTKAKYKDLSLLASAYKWEIPVCVQVAIGTDIIHQSPYADGKAIGDCSMRDFRIF
                     AEKVSELNGGGVFLNLGSAVIVPEVFLKALTIARNIYGEVQNFTTAVFDFNVHYRAKV
                     NVAERPVENGGKGYYFIGQNEIMVPLLLKAIME"
     gene            complement(2304..2885)
                     /locus_tag="PLG69_06275"
     CDS             complement(2304..2885)
                     /locus_tag="PLG69_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPI43152.1"
                     /translation="MAEKELLLHCCCAPCLIAPFYKLKAEGKELTAFWFNPNIHPLLE
                     YQKRRDTLRDFAVKKNIELLEQDEYGLMNFLQTTLKDINNRCQYCYETRLNAVAKTAK
                     EHGYSSFSTTMLYSKYQKHELIVDIALKMSAKYGIDFFYEDWRSLWWEGRKLAKEAGL
                     YRQQYCGCIFSEEERYREQIQRDRPKENKDLSN"
     gene            complement(3029..4174)
                     /locus_tag="PLG69_06280"
     CDS             complement(3029..4174)
                     /locus_tag="PLG69_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPI43153.1"
                     /translation="MDYFLTEEQLEMQEIARRIAEEKIRPVSEKYDEEGIFPWDIVEV
                     MKQSDLFAILVPEEYGGISGKVADLAVVTEELCAVDVGISLAFGATGLGMYPILIAGT
                     EEQKQKYLAQIAAGEQLAAFALTEANAGSDAGAIETTAKKEGDYYILNGTKQWITNGG
                     EAGIYCVFAMTDKTKGARGCSCLLVEKGTPGFNFGKKENKMGIRASATRELIFEDCKV
                     PAENLIGREGTGFITAMKVFDKSRPMVGAQSVGVARGAFEAAIKYSKERQQFGKPISS
                     FQGIQFMLADMATQIEAARALVMQTARMIDSGARNYSKESAMCKYFASDVAMRVTTDA
                     VQILGGYGYMKEYPVEKMMRDAKILQIYEGTNQIQRNIVAASLLKEY"
     gene            complement(4689..5915)
                     /locus_tag="PLG69_06285"
     CDS             complement(4689..5915)
                     /locus_tag="PLG69_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43154.1"
                     /translation="MKKFFLLLLLPVICIAFLQADYIYVVNSESRTLSRIDTETGTVN
                     NSFTQLGLTPNMLKVNSDFIYVVLSGANSIQMIDRNSGTTIRNIFIASSSNPWDVVED
                     NGYLYVSGMFTNKVYKISQESWTVIDQITVGTAPEGLEVCNGKLYVCNTGGYASGYAN
                     SSVSVIDLDSFSVIKNIPVWTNPQDIAVFGSQLHIVCTGNWSSISGAVDIIDSVTDER
                     IDRLLIGGNPWSVWISPQGFAYLGEGYNSGVYSYNASTLEILHNSSNPLIPGGIVISG
                     STDCIAVLYAPYSQNGTVYLRNNNWQPITQWTVALIPTDICYYSNETPVSDEELPLPQ
                     ITLYPNPVDKNGILNFKADNAFYGELKIYNLKGQKVLQVPVQKGEARIDLKGKGMGSG
                     LYFYHSGKAKGKIIVK"
BASE COUNT         1868 a         1283 c          962 g         1910 t
ORIGIN      
        1 aataatcctc aacaatttgc tgaatataca gatatgaatc agcttagttc cgatatcgcc
       61 aaattcagtt cttctatttt actgaataac ttatctattt caatgagtaa atatctggga
      121 agcagtattt ttctggacta tatgctgaaa ttacaagagg ccacaaactt gcaaaaccgc
      181 acccggattt tggtttccaa tgaggcatca attcgtctta tcttgcctaa aaattaccgc
      241 gtagcttata ctgttaccta cgaagctcaa gaaaaaaaga ccagccatga aattatgctg
      301 tcaaggttta ttagattctg gggactgtga tgaatgaaaa actgagaagg gagaattgct
      361 aattataaat aaacctcggt gctctcagta ttctcggtgt aaaaaagaat ggaaaatagg
      421 aagtgaaatc agttcaattc gtgttgctat taaatttatg ctatctgtta gggacatttt
      481 taataaaatc ctttgacaaa aagcacaacc tgaaaataaa ggtaaaaaat gataaaaatc
      541 ccgggaggat ataatgccgg ataatgttaa aaacgaaaat gcagtgaata ctaaaaaaac
      601 tcctaaaatg gctgattttt ccaccagagt ttccctggtt gaattgatta tgattttaat
      661 gctggtgggc ttggtttttg ttttttactt tggaatgaag caactccaaa tagataaagc
      721 caatgaagca attgcacagg aaaaatttga aaatataatc cccactttca aaaatatcat
      781 tgaggcaatg gaaacatacc gtaaagcaga tgaatttgga gattatcctg cctttttgga
      841 agaactgaac ctgggtgata tcaatacaaa cgattttaaa tttgaatatt cagctgatac
      901 ttatacaatc acagcaatta ctcaacctgc ttttggaaaa gcaggaatta aggttatcta
      961 caatctttcc gataaaagct ttacagttga agaccctata cccgataaaa aacctaccat
     1021 aaaggatgaa tggttgccac aagattaaac ccgccaataa gttaacgggg taaatgagtg
     1081 aagtgaaagg gtgaaacccg ctttataaat tgagggcaag gatagaaaat aaaattttcc
     1141 ggggggtatt cattcatcgc tgtggaagta ttgttcttgg gaagttatac tatgtcctta
     1201 ttcataaatc ataaaggaca gaaaaagttc cgtaggaacg acagatatta acaacgggtt
     1261 tcaaccccgt gataagaaaa ctgttatccc taaagtgact tttattaaat gacctttcaa
     1321 aatgaaaatt atattacagg tattgggaat ataaaagata aatccccaaa atcctgttca
     1381 tcccaaacct attattatta aatctaagtt atctgtgaga aaaaaactat tccataattg
     1441 ctttcaacaa caacggcacc attatttcat tctgccctat gaaataatac cctttccctc
     1501 cgttttctac gggtctttct gctacattta cctttgccct gtagtgcaca ttaaaatcaa
     1561 aaacggcagt agtaaaattc tgaacctcgc cataaatatt tcgggcaatc gttaatgctt
     1621 tcaaaaacac ctcgggaaca atcacggctg agcccaaatt taaaaacacc cctcccccat
     1681 taagttcaga aaccttttcc gcaaaaatcc gaaaatcccg catagaacaa tcaccaattg
     1741 cttttccatc cgcataagga ctttgatgaa taatatctgt tccaatagca acctgcacgc
     1801 aaacaggaat ttcccattta taggcagaag cgagtaaaga aaggtctttg tattttgcct
     1861 ttgtataaag ttctttgcca attgcctcac ctaaacccaa actctcttta aaacccttat
     1921 ttattgcttc attaattccc tttactgttt cttccgccat cccgaaagaa ccatctgata
     1981 aagcagaaga tacatcttcc gaggtcttgc cgaaactggc aatttcataa tcatgaatcg
     2041 ctacagcacc gttacacaca aaagcggaaa caaaatcctc ttccatcatt tctattagca
     2101 aaggtgccaa tccgcatttg ataatatgag ctcccagacc aattattatg ggtttatggt
     2161 tttctctggc ttttttgcag gtaagaataa gctccttcag gtctttggca gcaaggatat
     2221 cgggaaggca attgaaaaaa tcgttaacct tcactttccc ctttttggca aaagcattta
     2281 tattcacttt actataacgt tggtcaattg gataggtctt tattttcttt aggtctatct
     2341 ctttgtattt gctcacggta gcgttcctct tcactaaata tacatccgca atattgttga
     2401 cgataaagtc cggcttcttt agctaatttt ctgccttccc accataaaga tctccagtcc
     2461 tcataaaaga agtcaatgcc gtatttagcg ctcattttta acgcaatatc cacaatgagt
     2521 tcatgttttt gatatttact gtaaagcatt gtagtgctga aagaagagta accatgttct
     2581 tttgctgttt tggcaacggc atttaaacgg gtttcatagc aatattgaca gcggttatta
     2641 atatctttta gggttgtctg taaaaaattc atcagaccgt attcatcctg ttcaagcagt
     2701 tctatattct tttttacggc aaaatcgcgc aaggtatcac gccgtttttg atattctaaa
     2761 agcggatgaa tattgggatt aaaccaaaat gcagtcagtt ctttcccttc agctttcagc
     2821 ttataaaaag gtgcaatcaa acagggtgcg caacagcaat gcagtaaaag ttctttttct
     2881 gccataattc tatctcattg ttattcctgc gttcttgtaa taccccacaa gcaggaatcc
     2941 attcatttat cccccttcaa catagatccc gcattaagcg cggaatgata atcaccaata
     3001 tctgcaaaaa tcggattttg cctttctgtt aatactcttt cagtaaactt gcagcaacta
     3061 tgtttctctg aatctgatta gtgccttcat aaatctgtaa tatcttggca tcgcgcatca
     3121 ttttttccac aggatattct ttcatatagc cataaccgcc taaaatttga acagcatcgg
     3181 ttgtaactct catcgctaca tcggaagcaa aatatttgca catagcgctt tctttggaat
     3241 aatttctggc accactatct atcattcttg ccgtttgcat cactaaagcc cttgctgcct
     3301 caatttgagt agccatatct gccagcataa attggatacc ttgaaagctg gaaatcggtt
     3361 taccaaactg ctgtctttct ttggaatact ttattgccgc ttcaaaagct ccgcgagcaa
     3421 ctcctactga ttgagctcca accatcggac gggatttgtc aaaaaccttc atcgcagtaa
     3481 taaaaccggt gccttcacgc ccaatcaaat tctctgccgg aactttacaa tcctcaaaaa
     3541 taagctctct tgtagcggat gcgcgaatac ccattttgtt ttctttttta ccaaaattga
     3601 agccgggagt gcctttttcc acgagtaaac aggaacaacc acgcgcacct ttagttttat
     3661 ccgtcatcgc aaaaacacaa taaattcctg cttctccgcc atttgtaatc cattgttttg
     3721 tgccatttaa aatataataa tcgccttctt ttttggcagt ggtctcaatt gcaccggcat
     3781 cactgcctgc atttgcctct gttaaagcaa aagcagcaag ttgttctcca gctgcaattt
     3841 gtgccaaata tttctgcttt tgctcttcag ttcccgcaat caaaatagga tacattccca
     3901 aacctgttgc tccaaaagct aaagaaatac ctacatcaac agcacagagc tcttctgtaa
     3961 ctaccgctaa atctgcaact ttgccactta ttccgccata ttcttccgga accaaaatag
     4021 caaaaagatc gctttgtttc atcacttcta cgatgtccca agggaaaatg ccctcttcat
     4081 cgtatttttc ggaaacaggt ctaatttttt cctctgcaat ccgccgtgct atttcctgca
     4141 tttccaattg ttcttcagtt aaaaagtagt ccattatgta actcctatat tattttatgg
     4201 gttatatatt ttagcattag gcaaaaattc cctaaaactg tcaatctatt ttttccttct
     4261 ttaattacag ctcatcatcc acacatcctc aacccatcgt aaaggttggg ttgaggatgt
     4321 gtgaattgta ggcaagctat aggtactttg cggaaatgaa aaaatgagag tcaggagccg
     4381 tcactgtaga aataacttat atactaccca cgaatttatt tggcagtgag aactcgtgac
     4441 agccagaatg aaaaatgaaa aaattgagaa ttatcaataa atctctgtgt tctcagtgta
     4501 aaaaatcttc tcttcttttc tctttccctc tttgtaaaat taacctattt tctctttctc
     4561 tctttgtaaa attctcttct tttcactttt cctctttgta aaattctcct cttttctctt
     4621 tccctctttt tctctttgta aaataattct ctcggtgctc tcagtgaaaa aaaatctgct
     4681 taccttctct attttactat tatcttccct ttcgcctttc cactgtgata aaaatataac
     4741 ccgcttccca ttccttttcc cttaaggtct attcttgctt ctcccttttg tacaggcact
     4801 tgtaacactt tttgtccctt tagattataa atctttagtt ctccgtaaaa agcattatcc
     4861 gccttgaaat tgagaatacc atttttatca accggattag gataaagagt tatttgaggc
     4921 aaaggcaatt cttcatcaga aacaggggtt tcattgctat aataacaaat gtcagttggg
     4981 attaatgcaa ccgtccactg agtaatcggt tgccaattat tattccttaa atatacagtt
     5041 ccattttgac tatagggagc ataaagaaca gcaatacaat cagttgaacc gctgatcact
     5101 atccctcccg gaattaaagg attactgcta ttatgcaaaa tttccagggt tgaggcatta
     5161 taagaatata ctccgctatt atatccctct cccaaatagg caaaaccttg tgggctaatc
     5221 catacactcc aagggttgcc tccaattaaa agacggtcta tgcgttcatc ggttacggaa
     5281 tcaataatat ccacagctcc ggaaatggaa ctccaattac cggtgcatac tatatgcaat
     5341 tgtgaaccga aaacagctat atcctgaggg ttagtccaca caggaatgtt ttttattacg
     5401 ctaaaagaat ccaagtcaat aacagagaca ctgctattag cataaccgct ggcataacca
     5461 cctgtattac atacatataa ttttccgtta cagacctcca gaccttccgg ggctgtgcca
     5521 actgtaattt gatctataac tgtccagctt tcttgactga ttttatagac cttattagta
     5581 aacattccgc ttacataaag ataaccatta tcttctacta catcccaggg attggaactg
     5641 gaggcaataa aaatattacg gatagttgtt ccactatttc tatctatcat ctgaatgctg
     5701 tttgctccgg aaagaactac ataaataaaa tcactattaa ctttcagcat attcggagtt
     5761 aaacctaatt gagtaaaact attattcacc gttcctgttt cggtgtcaat tctgcttaaa
     5821 gttcgggatt cggaatttac cacataaata taatccgcct gcagaaacgc aatacagatt
     5881 actggaagca gaagaagtaa aaagaacttt ttcatcatat cctcatttag ttaaaaataa
     5941 caggtcttct cgaaagaata cggtaacctt ggagccatta ttaaaaaatc ctcttttgtg
     6001 tcaaatccca atatgcttct ggg
//
LOCUS       DAOPFL010000092         5966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_17457_length_5966_cov_99.013196, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000092 DAOPFL010000000
VERSION     DAOPFL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17457_length_5966_cov_99.013196"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="PLG69_06290"
     CDS             complement(<1..155)
                     /locus_tag="PLG69_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPI43155.1"
                     /translation="MRFEDQRIRLVNELKLEGITSPVVLNAFAKVPREHYVLPEYRDY
                     AYQNRPLP"
     gene            complement(158..928)
                     /gene="surE"
                     /locus_tag="PLG69_06295"
     CDS             complement(158..928)
                     /gene="surE"
                     /locus_tag="PLG69_06295"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPI43156.1"
                     /translation="MKILLVNDDGILAPGIRALAEELKNAGHKITIVAPDSERSAASH
                     SLSIRKELIVKKIAENEYAVSGTPVDCSVIALQKILTEPVDLIISGINAGQNMGEDVL
                     YSGTVGAAMEAALLGNRAIAVSINSYKNQRFEVAAYWMRKLLDLGIDKQAEAFGVLNI
                     NIPNLPLEEIKGIRLTRTGHRKYYNFVKIIKENEDGFVYEVGGDIPVWDNETGTDAEA
                     VNNGYISLTPLGFELTRSETFPRILNWLEENELLQLER"
     gene            complement(942..1295)
                     /locus_tag="PLG69_06300"
     CDS             complement(942..1295)
                     /locus_tag="PLG69_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43157.1"
                     /translation="MARKHAAILQELEIDEVEGYTEAQKRYYTSLGFKPYLNEDGKVK
                     WLRPDQHSLRINAKLKRPFVARLISHKKVQFPRRRRHRPALVKFVQRNWLFIIIVTIV
                     IVALVYIIRNPQLIF"
     gene            1500..1838
                     /gene="secG"
                     /locus_tag="PLG69_06305"
     CDS             1500..1838
                     /gene="secG"
                     /locus_tag="PLG69_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPI43158.1"
                     /translation="MVLYTIALIIHVIICVTLVLVILAQTSKGGLDSNLGGAAMNVFG
                     GSGASKFLKKWTQILAVVFAASCLFLAFLVKNINASALADIQSRQKKIKDTEQTAPSS
                     KPVTKPATTK"
     gene            1943..2030
                     /locus_tag="PLG69_06310"
     tRNA            1943..2030
                     /locus_tag="PLG69_06310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1977..1979,aa:Leu,seq:aag)
     gene            2192..2641
                     /locus_tag="PLG69_06315"
     CDS             2192..2641
                     /locus_tag="PLG69_06315"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPI43159.1"
                     /translation="MKRFSVVLLLILLCSSILAQPIKKNPNKAALCSIFPGGGQIYNE
                     AYIKAGAIIGIQTYLIITAIHNDAKVQDYKDKINSTSDEILLAEYRNKQKQYKEKRTR
                     DFWWMGITLAFSTLDAYIDAHLSNFKEEKEKIHIRFEEETLLLEYNF"
     gene            2657..3439
                     /locus_tag="PLG69_06320"
     CDS             2657..3439
                     /locus_tag="PLG69_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPI43160.1"
                     /translation="MRKSFFLILLLLFACTQSSKTISVTEFEHRIWELTNQQRSAYNL
                     PPLLYDEGLADLARLHSKNMFNFDFFAHKDHLGYLVDDRKKKYYPQLVVSSIGENLAK
                     FVNGARTFTPEEVVNGWMNSPEHKENILNTTYTHLGVGVFTQDEVLLATQNFAAPVVK
                     LLSEIPEKFDPLYQYQLLFEYMSPLPANELEAVLNFPDSRFKYYIDDRSYTMGLKPVP
                     LHWMTEKQLEVIVDFPAGRGNYSLCFGFNHSYLEDGIKIKVK"
     gene            complement(4103..4873)
                     /locus_tag="PLG69_06325"
     CDS             complement(4103..4873)
                     /locus_tag="PLG69_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPI43161.1"
                     /translation="MKLLLWSWNVNGLRAALNKGFIEIIQKEQPDILGIQETKLQEHQ
                     IPEELNTLNEYLIYWSHSLRKGYSGTGLFTKLLPLSFSTGFGNPEFDNEGRINIAEYT
                     DFIYFNIYFPNGQKDDERLNYKLRFYDRCLEVMEEKRTSGKIILVGGDFNTAHHPIDL
                     ANPKENEKTSGFLPIERAWLDKIAEMGWIDTFRYFDKSPEQYTWWSYRTKARPRNVGW
                     RLDYFWVNKEGIEHIKKAGIRQDIEGSDHCPVFVELKI"
     gene            5031..>5966
                     /locus_tag="PLG69_06330"
     CDS             5031..>5966
                     /locus_tag="PLG69_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPI43162.1"
                     /translation="MKNKKICLLLIPLLLLTGLSLFAQNEARNYALSDSLFTEIPDSL
                     KINVIETDSLFYKADSIHFEYDTEIIKLFGKPRINYQDTEIIADSITVDIKKERAFSY
                     GPTKMRDGEQLLLGSDVRYDVKTQTGILNNGYSLIEEGYYTGSELRKVDKDIYDIDGG
                     TFTNCDLEEPSFWFWSKQLRVYRGDKIVGKPVIGYVNHFPAFFFPFIIIPIRQGRHPG
                     FLIPSPDYNNVDGFVIRNLALYYPYRDYADFVLGFDIMEKTGWKGHFDTEYLKRYAFS
                     GNFNAAFQHRIDEYSTFDDWSLQGNHHQDLPEKSSL"
BASE COUNT         1892 a         1078 c         1045 g         1951 t
ORIGIN      
        1 ggtaagggac ggttttgata tgcgtaatca cgatattcag gtaaaacata atgctctcgc
       61 ggcactttgg caaaagcatt taaaacaacc ggagaagtta ttccttccaa ctttaattcg
      121 tttaccaatc ttatccgttg atcctcaaaa cgcatcctca tctctccaat tgcagtaatt
      181 cattttcttc cagccagtta agaatcctgg gaaaggtctc gcttcttgta agttcaaagc
      241 ccaaaggagt taaagatata taaccgttat taaccgcttc tgcatcggtt cctgtttcat
      301 tatcccaaac aggaatatca cctcctactt cgtaaacaaa accatcttcg ttttctttaa
      361 taatcttaac aaaattataa tacttacgat gacctgtacg ggttaaacga atacctttaa
      421 tctcttccag aggaagattg ggaatattaa tattcaaaac accaaacgct tcagcttgtt
      481 tatcaatgcc taaatccagc aatttacgca tccagtaagc tgctacctca aatctttgat
      541 ttttataaga atttatggaa acagctattg ctcgatttcc taaaagagct gcttccattg
      601 ctgctccaac tgttccggaa tataaaacat cttctcccat attctgtccc gcattaattc
      661 ccgaaataat taaatccacc ggttcggtaa ggattttttg taacgcaata acagaacaat
      721 caacaggtgt tccgctgaca gcatattcat tttcagcgat tttttttact attagctcct
      781 tacggatact caaagaatgc gatgctgcac ttctttcact atccggagct acaatcgtaa
      841 ttttatgccc ggcatttttc agctcttctg ctaaagctct tattccggga gctaaaatac
      901 cgtcatcatt aaccaataat atcttcaaag tttttctact cctaaaaaat aagctgagga
      961 ttacgaataa tataaaccaa cgctacaatg acaatagtta caataataat aaacagccag
     1021 tttcgctgaa caaactttac taaagccggt ctatgtcttc gtcggcgagg aaattgaact
     1081 ttcttatgag aaataagacg tgctacaaaa ggccttttta acttagcatt gatccttaac
     1141 gaatgctgat caggccttaa ccatttaact tttccgtctt cattcagata gggcttgaac
     1201 cccaaagaag tataatacct tttttgggct tcagtatatc cctcaacttc atctatttca
     1261 agttcttgca gtattgcagc gtgttttctt gccataaatt attcctcttt ttacattatc
     1321 aatttagaca actatttctg tcaagccaaa aaatctgatt atgcctgaat aaaaaagtga
     1381 taataaggga aaaacaagct ctgaattcag tagacataac tttttaattg acagaaacag
     1441 ggctgttttt tcaatgattt tttattagat taattaattc gtttagaaag gataaaataa
     1501 tggttttata cacgattgcc ttaattatcc atgtgataat ttgtgttact ctggttctgg
     1561 taatattagc tcagacctct aaaggaggtt tggattctaa tttgggtgga gcagcaatga
     1621 atgtttttgg cggaagtggt gcttccaagt ttttgaagaa atggacacaa attctggcag
     1681 tggtttttgc tgcttcctgt ttatttcttg cttttttagt taaaaatatt aatgccagtg
     1741 cccttgccga cattcaatcc cgccaaaaaa agattaaaga tacggaacaa acggctcctt
     1801 cgtctaaacc ggtaacaaaa cctgcaacta caaaataata taacttttat tttgaaaatg
     1861 cagttatttt gatagattat gcttggatta ctgctttcgc aggaataaga aggggcattt
     1921 cattctgaaa aaaatataaa aagcagaagt ggtggaattg gcagacacgc aagactaagg
     1981 atcttgtgcc tgttaaaggt gtgcgggttc aaatcccgcc ttctgcacca actttttaga
     2041 gatggagctt aaaaaagctc ctttttttta gtaagcattt taacagctgt atcttatttt
     2101 atatctgaac tctgatgtcc tcccgttaca ttattattga caatttaata agggaaaaag
     2161 ataaggaaaa aaagaaaaag caggagttgg tttgaaacga tttagcgtag ttctgctctt
     2221 aattctgtta tgcagttcaa ttttggcaca accgattaag aagaatccaa acaaagcagc
     2281 tttgtgttct attttccctg gaggagggca aatatacaac gaagcatata ttaaagccgg
     2341 tgccataatt ggaattcaga cctatttaat tattactgct attcataatg atgccaaagt
     2401 tcaggattat aaggataaaa tcaattccac aagtgatgaa atattacttg cagaatatag
     2461 gaataaacaa aagcaatata aggaaaaacg cacccgcgat ttttggtgga tgggaataac
     2521 tttagcattt tctacccttg atgcttacat tgatgcacat ctttccaatt ttaaggaaga
     2581 gaaggagaaa attcatatcc gttttgagga agaaaccctt ctattggaat ataactttta
     2641 gccggagaaa aaatcggtgc gtaaatcatt ttttcttatt ctcttactgc tttttgcctg
     2701 cacacaatcc tcaaagacca taagtgtaac ggaatttgaa catcgtattt gggaacttac
     2761 caatcaacaa cgttcagctt ataatttacc cccgcttttg tatgatgagg gtttggctga
     2821 tttagcccgg ttgcatagta aaaatatgtt caattttgat ttttttgcgc ataaagacca
     2881 tctcgggtat cttgtggatg accgaaaaaa gaaatactac ccgcaattag ttgtttcctc
     2941 tattggagag aacttagcca aatttgttaa cggtgcaagg acttttacac ctgaagaagt
     3001 agttaacggt tggatgaatt ctcctgagca caaagaaaat attttaaata ccacttacac
     3061 tcatttagga gtaggtgtat ttacgcaaga tgaagtgctt ttggctacac agaattttgc
     3121 cgctccggta gtgaagttgc tatccgaaat tcccgaaaag tttgacccct tatatcaata
     3181 tcaattgctg tttgaatata tgtcgccctt gcctgctaat gaactggaag ccgtattaaa
     3241 tttccctgat tcaaggttca aatattacat agatgacagg tcttatacta tgggcttgaa
     3301 accagttcct ttgcattgga tgacagaaaa acaattggaa gtaatagtag atttccctgc
     3361 cggcagaggt aattattctc tctgctttgg ttttaatcat tcctatctgg aggatggaat
     3421 taaaataaag gttaaataga gaaacgggat aacaggggaa atgaaaaaat aggaaggtga
     3481 aaaggggaaa tgtctatagg taaaaaggta aacccaaatt gaggaatata gttttcatat
     3541 cgttattgcg taaatatttt ccagtccact gtggatcccg gcaaaagcag aagtccagaa
     3601 atgttaatgc aggataactg ccagccaata agggaaaaca aggttaatag agaaaaggaa
     3661 ttggcaaaga aagaaagagc gagaccttta cctttttttt atgcagcaca ttatttccta
     3721 ccatgttgaa tctgttatac ttctttaaac cttctttaac gattctttaa cgaagttaag
     3781 gcattgttaa aggatcgtta aagagtcgtt aaagaaatga ccaggcaaca agtagttaaa
     3841 caaagtccac aaggtaattt tataaagcaa aaatgatgat gagagcataa cttctgtctt
     3901 gtaatatcgt tcaggaaaaa gctgcggcta ttatatgtct acgacctacg ggaccattag
     3961 aaaaattacc tattgtccgc tcaacttctt ctaatcttgg catcaagttc ttactttcag
     4021 cgttctattg tattcctcct attttttatc aaaggtctat ataatcatta tttatagctt
     4081 gttggtaaaa atgttgtata gattatattt tcagttcaac aaaaacggga cagtggtctg
     4141 aaccctcaat atcttgtctg attccagctt ttttaatatg ttcaattcct tctttattaa
     4201 cccaaaaata atccaatcgc caacctacat ttctgggtct ggcttttgtt ctatagctcc
     4261 accaggtata ttgttcaggg cttttatcaa aatagcggaa agtatctatc caacccattt
     4321 ctgcaatttt atccagccaa gcacgttcaa taggtaaaaa accgctggtc ttttcatttt
     4381 cttttggatt ggcaaggtct ataggatggt gagcagtatt gaaatcaccg ccaactaaaa
     4441 ttattttgcc ggaagttctt ttttcctcca taacttccag gcagcggtca taaaagcgga
     4501 gtttatagtt caagcgttca tcatcttttt gtccattggg aaaatagata ttaaaataga
     4561 taaaatcagt atattcggca atattaattc taccttcgtt atcaaattcc ggattgccaa
     4621 atcctgtgga aaagcttaaa ggtagcagtt ttgtaaataa gccggttcct gaataccctt
     4681 tgcgcaaact atgcgaccaa taaataagat attcatttaa agtgttaagt tcttcaggga
     4741 tttgatgttc ctgcaatttt gtttcctgaa ttcctaaaat atcgggctgt tctttttgga
     4801 taatttctat aaaaccctta ttcaaagcag cacgtaatcc attaacattc caagaccaaa
     4861 gtaataattt caaaaaaaac tccttttttc taacctcaac cggttattat gtttaagtaa
     4921 ttcctttttt aaaaaaggca ttcatctaat ataataattt agttattgct tgcaggcaag
     4981 ttattatcct gcttgacaca gagatggcat ttattttttt agattactaa atgaaaaata
     5041 agaaaatctg tctgttactg atacctttac tgttgctaac aggtctttcg ctttttgctc
     5101 aaaacgaagc aagaaattat gctttgtctg attccctatt taccgaaatt cctgatagct
     5161 tgaaaattaa tgtaatagag acggattcgc ttttttataa ggcagatagc attcattttg
     5221 aatatgatac ggaaattatt aaactttttg gcaagccccg cattaattat caggatacag
     5281 aaattattgc cgattccatc accgtagaca tcaaaaaaga acgcgctttc agctatggtc
     5341 ctaccaaaat gagagatgga gaacaattac ttttaggttc cgatgtccgt tatgatgtta
     5401 aaactcaaac cggtattttg aataatggat acagtttaat tgaagagggt tattacactg
     5461 gaagcgaact ccgtaaagtt gataaggata tttatgatat agatggtggc acttttacca
     5521 actgtgattt ggaagagcca tctttttggt tttggtcaaa acaattaaga gtttacaggg
     5581 gagataaaat tgtcggaaaa cctgtaattg gttatgtaaa tcactttccg gcattctttt
     5641 ttccttttat tataattcct atcaggcagg gaaggcatcc cggttttctt attccttctc
     5701 ctgattataa taatgtagat ggttttgtga tccggaattt agctttatat tatccttatc
     5761 gtgattatgc cgattttgtt ctgggctttg atattatgga aaaaaccggt tggaaaggcc
     5821 attttgatac cgaatacctg aaacgatatg ctttttccgg aaattttaat gctgcttttc
     5881 aacacagaat agatgaatac agcacttttg acgattggtc attacaggga aatcatcatc
     5941 aagaccttcc agaaaagtca tctttg
//
LOCUS       DAOPFL010000093         5960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_17481_length_5960_cov_58.987638, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000093 DAOPFL010000000
VERSION     DAOPFL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17481_length_5960_cov_58.987638"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>2798
                     /locus_tag="PLG69_06335"
     CDS             24..>2798
                     /locus_tag="PLG69_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43163.1"
                     /translation="MKRKFRTTLFLIVLFVLTATSLLGQSRVFDPSHIDVWLEDYLAG
                     SPTTAIIGTKVQVTLTDPDLGTSSQIETVYANFSQFGAYPEEIQMVWDADISKWKATY
                     QVAPGTLSGTQARVKIHSISQNENEQSIWDDAYFTVNNVPTILTGLEINTHITNPSPE
                     GYAIVGSSVVVSFTSEDLDAASFYIYSSDGSIMEYGPVAMVGPDADNVFTGTWVVNPY
                     EMILPAGNYMIKIIAYDYNLSPNPGEATAIDLIPIESIIISESEFISAYLGIDGNYTA
                     TVATTNNTISVHAEFDPIISKVTIDWGHTFPDATPVIYTLNNGILDIQYTIPDIRISS
                     RTILEVKISKIATAAGNYLDTSSLAGFIINKNQNGEEITVDFILPTITVNDNDLYYDN
                     SPATGWLRFSPNSPAVDDYTDTNPDYVNIKLDLEKWGAGTEDDIYGLQLRFEFESRTV
                     YYKDYTVNSPEVSYNAPTLTIKWDGLNNGTSLPEGTYGITLWKLWDEAGNVLDLSNYY
                     IISDEFPPHSHLLNASSNEPQVILNRMHVVIDNTPLAFVPTANNSDIVTPDDLTFTRH
                     TKTDHYIIDGTPTNNFVYDYWEDNYTLANFSFQAKRDFTVDSNDLRRETGKYWIVLQK
                     ENATAKWYWNDATSTWNDFTEFNFPANLTPLTFTTDTSQNSATVAFNWNAKDPLFTDG
                     TYIMNAYLMDNAGNIIVSNPVNIIRETLVEDQTYTGNIALITGIEVTSRHYGGDFPLT
                     VVENDIPNFYLNTDYYDNSRTQDQIEIEVTVNNSDYLVETNDAVTLDLSNLGLGIMSL
                     AKTNFVNNKAKFIITNAQLASIPDNKAGTYTIGPADGAFNLPIISRSIIPKPEDLNHI
                     HEAGTDELAKFKLVRPDDPIYPVQGSVSVSDHSFSPGHPSHTYNALANPANDGIQDST
                     IVTISVPASTY"
     assembly_gap    2799..2898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2989..>5960
                     /locus_tag="PLG69_06340"
     CDS             2989..>5960
                     /locus_tag="PLG69_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43164.1"
                     /translation="MPIPAVSSANNNFEITLKTNEPLRTTNLNQLGNHTFHNSGWNVA
                     VLADTNNVIIPGASGEVNSITDLGNNTYKLSIKVNGINNIESPYAKILLRLPWDEAGN
                     PGRYNDPSYPLTADLFHNDSAEISIPIHILNARPWISKIQFTNYQSIGVVEYNEEGTV
                     TLSTQGYINNYTNSSGTLVAWISGGIYRNIVTTFMADVSSISTMANASALPGTAVQEG
                     TLWKITWNITNIRTDLVHNTPKVITISAKTAEGNNQLHLTSRTITLIVDKNSPVVSST
                     TFDGTNNSVVADGTAKPFTFNVSDALANIYWDTANSISLTFTNGVTHSGLTLNPTSGT
                     VTGNITLPANTAIKYFIATFKVKDRVNNELTINRYINVVPSPQPTNITLKSAFPLVET
                     SYFRPGTDIHVIFNVTNYQRADKVEVKLINQTTGLQVGTTQTITSPTTGSFDRTFSAV
                     IIPTSNVLRADIIVTYTKYDTGTSTTLNTNATNSASSPNTLIADSVLPVITATTGSLP
                     YLTAGSSQVLSFDITDATSGVNWSSPTISFNPATGISIGALDTSVTGKARWTVTVNEN
                     IASQRVSVTVTAKDKVDNTQTLTRYLNIMPIPVISNILVSDNSTGNLEPYNWFVPGHD
                     LKVSFTVSNPQRVQQLVVTITANGAPLDSKTYNVGDQIFLNSNMNVIFPALTAADLDG
                     KVIRATITGHTLSYSDPSNNSPSTTLLINSGSYDEINVDTQPVIQSVQFFLTASSNDA
                     IDFLMPTMEDLKVVVKVKSKNNISNPALTLINQIEGTTQVVLDPNSPTIEGSGVDRIL
                     TYIYNVDVTELDVNIPELYKLANFKVTTGTNYGYQATDYFYDMIVLGDALVDQYGIIP
                     PTPNDRNYVDVGRAPNHWFAPENNLITEYRFISSKDSTSVGIMADFDRIEDNVPDNWN
                     TTPVLTTEIIPLVLSPGRPVNVYRYLAKWDIVPDYSSVWNAYADGEPIQIDYKYWSFL
                     PAIIEDTTMVRVDKEVPT"
BASE COUNT         1886 a         1046 c         1018 g         1910 t
ORIGIN      
        1 ttattaaaaa aagcgaggtg caaatgaaac gcaaatttcg cacaacacta tttctcatag
       61 tgttattcgt tcttaccgcc acttctctat taggtcaaag tagggtcttc gatccttctc
      121 atattgatgt ttggcttgaa gattacttag ctggttctcc aacaacagcc ataataggga
      181 caaaagttca agttacccta accgaccctg atttggggac ttcatcccag attgaaacgg
      241 tttatgctaa ttttagtcaa tttggagcat atcctgaaga aattcagatg gtttgggatg
      301 ctgatatttc aaaatggaaa gcgacttatc aagttgcacc aggaactttg agtggaaccc
      361 aagctcgggt caaaattcat agcatttctc aaaatgaaaa cgaacaatca atttgggacg
      421 atgcttactt tactgtaaat aatgttccaa ctattctgac tggtttagaa attaacaccc
      481 atattactaa tcccagtcct gaaggatatg cgatagttgg ttcaagtgtt gtagttagtt
      541 ttacaagtga agatttagat gcggcttcat tttatattta cagttctgat ggtagtataa
      601 tggagtatgg tcctgtggca atggttggtc ctgatgctga taatgttttt accgggacct
      661 gggttgttaa tccctatgag atgatattac ctgctggtaa ttatatgatt aaaataattg
      721 cctatgatta caatttaagt cccaatccag gtgaagcaac tgctattgat ttaataccta
      781 ttgaaagtat aattatctca gaaagtgaat ttattagtgc ttatttggga atagacggga
      841 attacactgc tacagtagct actacaaaca atactatatc tgtacacgca gaatttgatc
      901 ctattataag caaagttact attgactggg gacatacttt tccagatgca acaccggtaa
      961 tatatacttt gaataatgga attttagata ttcaatatac aattccagat ataagaatat
     1021 cttcccgaac aatccttgag gttaaaattt ccaaaatagc gactgctgcc ggtaattatt
     1081 tagatactag tagtttagct ggtttcatta tcaataaaaa tcaaaatggt gaagaaataa
     1141 cagttgattt tatacttccc acaataactg ttaatgataa tgatctttat tatgataata
     1201 gtcccgctac aggttggcta agattttcac ccaattcccc agcggtagat gattatactg
     1261 atactaatcc tgattatgtt aatataaaac ttgatttgga aaagtgggga gctggaactg
     1321 aagatgatat ttacggtttg caacttcgtt ttgagtttga aagtagaact gtatattaca
     1381 aagattatac agtaaattct cctgaagtta gttacaatgc tcctacttta actatcaaat
     1441 gggatggttt aaataatgga acatctcttc cagaaggtac ttacggaatt actctgtgga
     1501 aattatggga tgaagcagga aatgttttag atctcagtaa ctattatata atttctgatg
     1561 aattcccacc tcattctcat ctgctaaatg caagtagtaa tgaaccacag gtaattctga
     1621 acagaatgca tgtagtgatt gataatactc cacttgcttt cgtacctact gctaataatt
     1681 cagatatagt tactcccgat gatctgacat ttacccgtca tacaaaaact gatcactata
     1741 ttatagatgg aacacccact aataattttg tatacgatta ttgggaagat aattatactc
     1801 tggctaattt cagctttcag gcaaaacgag attttactgt agatagtaat gatttgagac
     1861 gggaaactgg caaatattgg attgttttac aaaaagaaaa tgctactgca aaatggtatt
     1921 ggaatgatgc tacaagtacc tggaatgatt ttacagaatt taattttcct gctaatttaa
     1981 cacctcttac attcactact gatacatcgc agaattctgc aactgtagct tttaactgga
     2041 atgcaaaaga tcctctgttt acagatggaa cttatataat gaatgcttat ctgatggata
     2101 atgccggtaa tattattgtt tccaaccctg taaatattat tcgtgagaca ttggttgaag
     2161 atcaaacata tactggtaat attgcattga ttacaggaat tgaagttact tctcgccatt
     2221 acggagggga ctttcctctt acggtagtag aaaatgacat acccaatttc tatttgaata
     2281 cagattacta tgataatagt agaacgcaag atcagataga gatagaagta actgtaaata
     2341 atagcgatta tcttgtggaa acaaacgacg ctgttacctt agatttgagc aatctgggat
     2401 taggtattat gtctttagcc aaaactaatt ttgttaataa taaagctaaa ttcattatca
     2461 ccaacgctca gcttgcttct atcccagata ataaagccgg aacttatact ataggacctg
     2521 ctgatggtgc atttaattta ccaataattt cccgaagtat tattcccaaa ccagaagatt
     2581 taaatcatat tcacgaagca ggaactgatg agttagctaa atttaagctt gtccgacccg
     2641 atgatcctat ataccctgtt caaggtagtg tttctgtttc cgatcatagc ttttctcctg
     2701 gtcaccccag ccatacttat aatgcattag ctaatcctgc taatgacggt attcaggatt
     2761 caacaattgt aactatttct gttcctgctt caacttatnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnngc tatagataat gaaaatccga aacttgagag tattcctgag
     2941 atttacacct atgatcataa cgcaaggtca ataacatttg aagagggtgt gcctattcct
     3001 gcggtttctt cggctaataa taattttgaa ataaccttaa aaacaaatga accacttaga
     3061 actaccaatc tgaatcagct tggaaatcac accttccata attccggatg gaatgtagca
     3121 gttttagctg atactaataa tgttataata cctggtgctt caggggaagt taattctatt
     3181 actgatttag gtaataatac ttataaactt tccattaagg tgaatggcat taataatata
     3241 gaatctcctt atgctaagat tcttttacgt ttaccttggg atgaagcagg taatcctggc
     3301 agatacaatg atccaagtta tccattgact gctgatttat tccataatga ttccgctgag
     3361 ataagtattc ctattcatat tttaaacgcc agaccctgga ttagcaaaat tcaatttact
     3421 aactatcaat caattggagt agttgaatac aacgaagaag gaactgtaac tttatctact
     3481 cagggatata ttaataatta tacaaatagt tccggtacat tagtagcttg gatatccggt
     3541 ggtatttatc gtaatatcgt tactaccttt atggctgatg tttcctctat ttcaacgatg
     3601 gcgaatgcat cagctcttcc aggaactgct gttcaagaag gaactctttg gaaaataact
     3661 tggaatatta ctaatattag aactgacctg gttcataata ctcctaaagt aattactatt
     3721 tctgctaaaa ctgcggaagg taacaaccaa ttgcatttaa ccagcagaac cattacatta
     3781 attgtagata aaaatagccc ggttgttagc tctaccacat ttgatggaac taataatagt
     3841 gttgtagctg atggaactgc taaaccgttt acattcaatg tatccgatgc tctggctaat
     3901 atctattggg atactgcaaa ttcaatttca cttacattta ctaatggcgt aacccacagt
     3961 ggcttaactt taaatccgac ttctggaacg gtaactggca atattacttt gccggctaac
     4021 actgcaatta agtattttat tgccactttt aaagtaaagg atagagtaaa caacgaactt
     4081 acaataaata gatatattaa tgtagttccc agtcctcaac ctacaaatat tactttaaag
     4141 agcgctttcc cattagtgga aacatcttat tttagacctg gaaccgatat tcatgtaatt
     4201 tttaatgtaa ccaactatca aagagccgat aaggtagaag ttaaattaat aaatcaaact
     4261 accggtttac aggttggcac tacccaaaca attacctctc ctactacagg ttcttttgat
     4321 agaacattct ctgcggttat catacctacc agcaatgttc tacgcgctga tataattgta
     4381 acttatacta aatatgatac aggaacttcc actactctaa atacgaatgc aacaaactcc
     4441 gcctccagtc ctaatactct gattgcagat tctgtacttc ctgtaataac tgctacaacc
     4501 ggttccttac catatcttac tgcgggtagc agccaagtat taagctttga tattactgat
     4561 gctacttcag gagtgaattg gtcaagtcct acaatttcct ttaatcccgc tacaggtatt
     4621 agcattggtg cacttgatac atcagttacg ggaaaggcaa ggtggactgt tacggttaac
     4681 gagaatatag ctagtcaaag agtaagtgta actgttacag ctaaagataa agttgataat
     4741 acacaaactt taactcgcta tcttaatatt atgccaatac ccgtaatttc taacatttta
     4801 gtttctgata acagcactgg aaatttggaa ccgtataact ggtttgttcc tggacacgat
     4861 ctgaaagtca gttttactgt ttctaatcct caacgtgttc agcagcttgt agtaactatc
     4921 actgctaatg gtgctccact tgattctaag acatataatg tgggtgatca aattttctta
     4981 aacagtaata tgaatgttat attcccagct ctaactgcag ctgatcttga tggaaaagtt
     5041 ataagagcaa caataacggg acacacatta tcatattctg atccctccaa taattctcct
     5101 tctacaacac tgcttataaa cagtggttcg tatgatgaaa tcaatgttga tacacaacca
     5161 gtgattcaaa gtgtgcaatt cttccttact gcaagctcca atgatgctat tgactttcta
     5221 atgcctacta tggaagattt aaaagtggta gttaaggtta aatctaaaaa taatatttct
     5281 aatcctgctt tgaccttgat aaatcagatt gaagggacta cacaagttgt tcttgatccc
     5341 aattctccta ctattgaagg ttcaggtgta gatagaatat taacatatat ttataatgta
     5401 gatgtaaccg aacttgatgt taatattcca gagttgtata aactagccaa ttttaaagtt
     5461 acgactggaa ccaattacgg ttatcaggca actgattatt tttatgatat gattgtatta
     5521 ggtgatgctc ttgtagacca gtatggaatt atacctccaa ccccaaatga tcgtaattat
     5581 gttgatgtgg gtagagcacc caatcattgg ttcgctcctg aaaataatct tatcacagaa
     5641 taccgtttta taagctccaa ggattcaaca tcagttggta ttatggctga ttttgacagg
     5701 attgaagata atgttcctga taattggaat acaactcctg ttcttactac tgagattatt
     5761 cctttagtat tatctcctgg gcgacctgtt aatgtctatc gttatttagc taaatgggat
     5821 attgttcccg actactcttc tgtttggaat gcttatgccg atggcgagcc gattcaaatt
     5881 gactataaat attggagttt tctacctgca ataattgaag atacaactat ggtaagggta
     5941 gataaggaag ttcctactta
//
LOCUS       DAOPFL010000094         5942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_17559_length_5942_cov_103.118566, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000094 DAOPFL010000000
VERSION     DAOPFL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17559_length_5942_cov_103.118566"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="PLG69_06345"
     CDS             complement(<1..1778)
                     /locus_tag="PLG69_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPI43165.1"
                     /translation="MPKFLYLWLKRILCKFGNRELHIPLPVFGLGCGVLGLALPKVSL
                     KMGEYASKLLKALEYRGYDSTGAAFQGNTTEITLLKDVGAPSTLVKTLGIEKQSGKIF
                     CGQVRWATFGFVDKKNAQPHEVKCKRHIYGAHNGNITNTRELKSFLTKEGHFVQSDND
                     GEMLVHTVEHYFDIEMDKAGNPTDIDLRKNCMRKAIIQTADKLVGSYAAVIVDPVTET
                     CWAIKAGSSLYFGIGTLEDMPFALASSDLTAVLRFTKQLVNLREGEFIEYNADNYQVY
                     AQKNLKFKRLNQPDEIWYTGDKIPAKPIYSKLRAEDVELLPEYEYFMEQEIYAQSETT
                     GKLIKLFQGGSNSGKRVLSLLAKAGVRDILLEQVQLFLTENNFAERHRIFTELLNSET
                     FNHFFNQVFSTYREFFDVAVKEDFEKKYFFSNEKNFFLEMVNGEYDLKKISLAKTMDA
                     LAEEMNVKDFNASVDDFLQLVKNTILNSRNAYSIACGTSFHATKIAALFFNSIAGMEI
                     IPILPGDFRGEYSNCIKDNDLIIGVSQSGETKDLIDIFNDIDAKELNVRKVVLVNNMN
                     STLGQEKSDVAIPILCGPEIAVPATK"
     gene            complement(1790..2494)
                     /gene="ispD"
                     /locus_tag="PLG69_06350"
     CDS             complement(1790..2494)
                     /gene="ispD"
                     /locus_tag="PLG69_06350"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPI43166.1"
                     /translation="MDFLNNTTAIITAAGSGKRLPGNRKKQFRELEGIPILIRSMEPF
                     MASELIDNLVITVPESDIAHTEALIEQWFEGINKPYIVIAGGLERQDSVFSALQVCPE
                     GTEYVAIHDGVRPFVSQELLEILFSAVVIDKAVIPAGKIKHTVMQVEGNYAVNTLPRH
                     QLINVFTPQVFAYSIIMEAYHQAYKDSFFSTDDASLVQHLGMKIRYIWDNDFNIKVTD
                     EADLFFAKQLIEKKKL"
     gene            complement(2482..3432)
                     /locus_tag="PLG69_06355"
     CDS             complement(2482..3432)
                     /locus_tag="PLG69_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPI43167.1"
                     /translation="MIKYKHLFGPVMSRRLGISLGIDLVPYKYCPLNCVYCEVQRTTH
                     QVTKREAFFEMREILAELDSFMSTNPHLDYITFSGAGEPTLNSLLGQIVNYIKAKYPS
                     YKLALLTNGILFSDPEVRKEVLPCDIVLPSLDSATQEGFEKINRPCPEMKVEELIEGL
                     IHFRQEYKGIVWLEVFLVSGINTIPQEISALAKAINNIKPDLVQLNCLDRPGAEDWVK
                     PIPLHTLKQVKAQLSQQVCVPVEIIAKVKYQPTESQLDSEIIELLHNTLKRRPSTAED
                     LSAMLDIHINEISKVLSQLYQEKKITAQRESRGVFYKWIS"
     gene            complement(3434..5686)
                     /gene="bamA"
                     /locus_tag="PLG69_06360"
     CDS             complement(3434..5686)
                     /gene="bamA"
                     /locus_tag="PLG69_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPI43168.1"
                     /translation="MRRIILCSILILLLGCLVQNLYAVGETIYEIKVIGAVNIDPELV
                     TSALSFRVGDTLDPEAVAKSIRNLYRMGIFSDIQVESEPYRTGVTILIKIQENPIVSS
                     IEYIGFKAVKQERIDELVNVKVGSYWSEGVKNQLLNKLKNEYATKGFSNAEIQIFESR
                     LPNNKIGLKVQVNEGKKVSIKQITFVGNSYFEDKTLQKKMKTKPASFLRSGHFEQEKF
                     AADLQALTAFYKKNGFIDVVIGPYEIQPLGEKYIEIVINVYEGTKYNFGGITIEGNDF
                     FSSEELQDIFTMKIGEPFDQEFFDNEMRKIYTKYFDEGFIYVSIIPNYVKEGDQLIVN
                     LKINENNRARIHQIHITGNRKTKEKVIRRQLEVSPGDYFRQTQVMRSQQNIYNLGFFE
                     QDIRLDYTPINKDGDIDLQLDVIDRASGTANGGVGYNSQDKFVGQLSVSQNNLFGNNW
                     STSLTWEFGGNTQNFEFAFTNPNLLDTDILLGSNLYYTKKTWSSFYYEIYTRGAGIRV
                     GQTIPWIDKTRAVAGYSLYSKKYRITNMNAIMADSTANANLIELSKLNWRYTSVFSLT
                     LSRDTRDNVFFPTKGSQFTLYSEVAGGMFGGDFDYFKEIAQVNWYMDTWEKLILRTKW
                     RFCYVTPYGRSKDAPPDEKFYLGGTGADGIRGFPDSSIGPAGGGTRAIIFSTELGYPL
                     GTDQIIAVAFFDAGNSYNKMRDFNFLNFKKGTGLGIRIRSPFGLIGFDYAYNINDGGW
                     EPHLQFGTTF"
BASE COUNT         1773 a         1259 c          995 g         1915 t
ORIGIN      
        1 ctcttggtgg caggaacggc aatttccggt ccgcataaaa taggaattgc cacatcactt
       61 ttttcctgac ccaaagtgga attcatatta ttcactaaaa ccactttacg gacattcagt
      121 tctttggcat ctatatcgtt gaatatatct atcaggtctt tggtttcccc cgattgagaa
      181 acgcctataa taaggtcatt atcttttatg cagttggaat attcacctct aaaatcgcca
      241 ggtaaaatgg gaattatttc cataccagca atagaattaa agaaaagagc agcaatctta
      301 gtagcatgga aactggttcc acaggcaata gaataggcat tgcggctgtt taaaatagtg
      361 tttttaacca gttgtaaaaa gtcgtctacg ctggcattaa agtccttcac attcatttct
      421 tcagctaaag catccatagt tttagccagc gagattttct tcaaatcata ttctccattc
      481 accatttcca gaaagaagtt cttttcgttg gaaaagaagt atttcttttc aaaatcctcc
      541 ttcacggcaa catcaaaaaa ctcccgataa gtgctgaaaa cctgattgaa aaaatgatta
      601 aaggtttcgg aatttagaag ttcggtaaaa atcctgtgcc gttctgcaaa attgttttcc
      661 gttaaaaaca gctgaacctg ttctaataga atatcccgca ctcctgcttt tgccaataat
      721 gataacactc tttttccgct attggaacca ccctgaaaga gtttaatcag tttgcccgta
      781 gtttcacttt gagcataaat ttcctgctcc ataaaatatt catattcagg caaaagttca
      841 acatcttcag ctcgcagttt ggaataaatt ggcttggcag gaattttatc acctgtatac
      901 catatttcat cgggttgatt taaacgcttg aattttaagt ttttctgtgc ataaacctga
      961 tagttatctg cattatattc aataaattca ccctcacgca aatttaccag ttgcttagta
     1021 aaacgcaaaa cagctgttaa atcggaagat gccaacgcaa aaggcatatc ttccaaagtc
     1081 ccgatgccaa aatataaact gctgcctgct ttaatagccc aacaggtctc cgtaacagga
     1141 tcaacaatta cagcggcata gcttcccaca agtttatctg cggtctgaat tattgcctta
     1201 cgcatacaat tcttacgtaa atcaatgtcc gtaggatttc ctgctttatc catttctatg
     1261 tcaaaataat gttctacagt atgcactaac atttcaccat cgttatcgct ttgcacaaag
     1321 tgaccttctt tcgttaaaaa ggactttaac tctctggtat tagttatatt accattatgt
     1381 gcaccataaa tatgcctttt acatttaact tcgtggggct gagcattttt cttatccaca
     1441 aagccaaaag tagcccaacg cacctgtccg caaaatattt taccgctctg tttttctatg
     1501 cctaaggtct ttactaaagt gctgggagcg ccaacatctt ttaacagtgt tatttcggtt
     1561 gtatttccct gaaaagctgc tcctgtgctg tcataacctc tatattccaa tgcttttaac
     1621 agtttggagg catattcacc catttttaaa gagaccttag gtaatgctaa acctaaaaca
     1681 ccgcatccca acccgaaaac aggtaaagga atatgcagtt ccctgtttcc gaatttgcat
     1741 aatatacgtt ttaaccataa atacaaaaac ttaggcattg ttatgtcctc tataatttct
     1801 ttttttctat taactgcttg gcaaagaaaa ggtctgcttc atcggttact ttgatattga
     1861 aatcgttatc ccaaatatag cgaattttca ttcccaaatg ctgaaccagg gaagcatcgt
     1921 cggtagaaaa aaaactgtct ttatatgcct gatgatatgc ttccataatt atagaatagg
     1981 caaaaacctg aggagtgaaa acattgatga gttgatgccg cggaagagta ttaactgcat
     2041 aatttccttc tacttgcatc acggtatgtt ttattttccc agcaggaata actgctttat
     2101 caataaccac tgccgaaaaa agaatttcca gcaattcctg cgaaacaaaa ggacgcactc
     2161 catcatgaat tgctacatat tctgttcctt caggacaaac ctgtaaagcg gaaaaaacag
     2221 aatcctgcct ctccaaccct ccggcaatta cgatataggg cttatttatt ccttcaaacc
     2281 attgttctat aagagcttct gtgtgggcaa tatcgctttc cggaacagta attacaaggt
     2341 tatcaattag ttccgaagcc ataaagggct ccatacttct aatcaaaata ggaattcctt
     2401 ccagttccct aaattgtttt tttctgtttc ccggtaggcg tttaccactt cctgctgcgg
     2461 taataattgc ggttgtatta tttaagaaat ccatttataa aaaacccccc gactctctct
     2521 ttgggcagtt attttctttt cctgatataa ttgacttagc actttgctga tttcattaat
     2581 gtgaatatcc agcatagcag aaaggtcttc tgcggtagaa ggacgccttt taagcgtatt
     2641 atgcaaaagt tcaataattt cggaatccag ttgactttcc gtaggttgat atttaacttt
     2701 ggcaataatt tccacaggta cacaaacctg ttgggaaagc tgagctttaa cctgcttaag
     2761 tgtatgcaaa ggaatgggtt taacccaatc ttccgctccg ggacggtcta aacaatttaa
     2821 ctgcaccaaa tccggcttaa tattattgat agcttttgcc agagcggaaa tttcttgcgg
     2881 aatggtgtta attcctgaaa ccagaaaaac ttccagccaa acaattccct tatattcctg
     2941 tctgaaatga attaaaccct caataagctc ttccactttc atttccggac agggacgatt
     3001 gattttctca aaaccctctt gagtagcaga atcaagtgac ggcaaaacaa tatcacaggg
     3061 aagaacttct ttacggactt ccggatcgga aaacagtatg ccattcgtaa gcaaggcaag
     3121 tttatagctt gggtatttag cttttatata gtttactatc tgccctaata aactgtttaa
     3181 tgtgggttca ccagctccgg aaaaagtgat ataatccaaa tgagggttgg tagacataaa
     3241 actatccaat tcagccaaaa tttccctcat ctcaaaaaat gcttcccgtt tagttacctg
     3301 atgtgtagtt ctttgcactt cacaatacac gcaatttaag ggacaatatt tatacggaac
     3361 aaggtctata cctaaagata tacctaaccg acgggacatt acaggtccaa aaaggtgttt
     3421 atacttaatc atcttaaaag gttgttccaa attgtaaatg gggttcccaa ccgccatcgt
     3481 tgatgttata ggcataatca aaaccgatta aaccgaaagg actgcggatt ctgattccga
     3541 gtccggttcc ttttttaaaa ttgaggaaat tgaaatcacg catcttatta tagctgtttc
     3601 cggcatcaaa aaaggcaact gcaattattt gatcggttcc taagggataa cctaattccg
     3661 tagaaaaaat aattgctctt gttccgcctc cggcaggacc aatggaacta tcgggaaaac
     3721 ctcttattcc atctgcacct gttccgccca aatagaattt ttcatcagga ggagcatctt
     3781 tagacctgcc ataaggagta acataacaaa aacgccattt ggtgcgtaaa ataagtttct
     3841 cccaggtatc catataccag ttaacttgag caatttcctt gaaataatca aaatcaccgc
     3901 caaacatacc acctgcaact tcactgtaaa gagtaaactg acttccttta gtagggaaaa
     3961 acacattgtc ccgtgtgtct ctgcttagcg ttaaactgaa cacactggtg tatctccaat
     4021 tcaatttact cagttcaatc aagttggcat tggcagtgga atctgccata atagcattca
     4081 tattggttat tctgtatttc ttggaataga gagaataacc ggctacagct ctcgttttat
     4141 ctatccaggg aatggtttga ccaaccctga ttcctgcacc acgagtataa atctcgtaat
     4201 agaaagagct ccatgtcttt ttcgtgtaat agagattgct tcccaaaaga atatcggtat
     4261 ccagcaaatt aggattggtg aaagcaaatt caaagttttg ggtgttgcct ccaaattccc
     4321 aggttaaact ggtagaccag ttattcccga atagattatt ttgggacact gatagttgcc
     4381 caacaaattt atcctgagaa ttataaccca ctcctccatt ggcagttcca cttgcacggt
     4441 ctataacatc aagctgtaaa tctatgtccc catctttatt gatgggagta taatccaaac
     4501 gaatatcctg ttcaaagaaa cccaaattgt aaatgttttg ttgacttcgc attacttggg
     4561 tctgacgaaa atagtcacca ggtgaaactt ccagctgacg ccggatcact ttttccttgg
     4621 tttttctgtt acctgtaata tgtatctggt gaattcgggc acggttattt tcattgattt
     4681 tcaggtttac gattagctgg tcgccttctt taacataatt cgggataata gacacataga
     4741 taaacccttc atcaaagtat ttggtataga tttttcgcat ttcgttatca aagaactcct
     4801 ggtcaaaggg ttcgccaatt ttcatcgtga aaatatcttg tagttcttca gaagagaaaa
     4861 aatcattccc ttcaatagtt atacccccga aattatattt ggttccttca taaacattaa
     4921 taacgatttc aatgtatttc tcccccagtg gttgaatttc gtagggacct atcactacat
     4981 ctataaatcc gtttttctta taaaaagcgg tgagtgcttg cagatcagca gcaaattttt
     5041 cctgctcaaa atgacctgag cgaaggaaac tggcaggttt ggttttcatc tttttctgca
     5101 aggtcttatc ttcaaaatag ctattcccta caaaggttat ttgcttgata gagacctttt
     5161 taccttcatt tacctgaacc ttcaaaccaa tcttgttgtt aggtaaacgg gattcaaata
     5221 tctgaatttc ggcattactg aaacccttgg tggcatactc atttttcagc ttgttcagta
     5281 actgattttt aactccttca ctccagtaac tgccaacctt aacatttacc agttcatcta
     5341 ttctttcctg ctttaccgct ttgaaaccaa tatattcaat actgcttaca atcggatttt
     5401 cctggatttt tattagtata gttactcctg ttctataagg ttcagattcc acttgaatat
     5461 cagaaaatat tcccattcta tacaaattac gaatggattt tgccacggct tcaggatcaa
     5521 gagtatctcc aactctaaaa ctgagtgcgg aagtaaccag ttccggatct atatttacag
     5581 ctccgattac ttttatttca tatatggttt ctccaacagc atacaggttt tggacaagac
     5641 agccaagcag caaaattaag atgctgcata gtattattct tcgcattatt cctccactca
     5701 agagttaata gccagaattg taaatagccc tttttagtaa agacattttt ttgttttatc
     5761 ctgctaattc ctttagggca tttttaaaaa cacatattta aggtttagct ttatatctgt
     5821 ttttattaca agctattatc cccttttttc tgttcttccc taattgcatt gcttacccat
     5881 tgcttaccca ttactgatgg cattagcaat gagtccgtat tgcgtaacta atgcttcttg
     5941 gc
//
LOCUS       DAOPFL010000095         5878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_17839_length_5878_cov_74.436030, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000095 DAOPFL010000000
VERSION     DAOPFL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17839_length_5878_cov_74.436030"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4112
                     /locus_tag="PLG69_06365"
     CDS             <1..4112
                     /locus_tag="PLG69_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPI43169.1"
                     /translation="DGGTEHYLAVLDLQSRTTVQVLDIYPHYAQGVTIGPDGKVLIND
                     YENGYVHQYILNFVTGNLSYSGVSIPVASCLNTVISPGGNFAIACSYNRDTKVLKLNA
                     DNTIDVIQNLGLSSQSAVYSSDGQIALIGEYSSSNDILAKYNVQGNGSLVWQQSFNLP
                     FYSESGYYGVDVLALSTDNSKAYITDFGAEANNILVNINLENGTQQNLTLPDPTGLAI
                     GTMHLTAYFTATITNTQNSTQVQFHQHCSGNPDSYLWNFGDGQTSTEPNPYHIYYTPG
                     LYTVTLTVYKNGESDSYSQTIEAMFNTQIHLTLDGSPYYYSATLAISEGSSLIIDNGV
                     TVNFGPQAGLEVWGSISANGVTFSGSETEGWKGIVLHSPVSPVAFNNCNIYNAVVGLK
                     IINSSFTITNLLISKTHSFTGEIGLKVQGASNMVLNNVQVIGYIMGIVFENEARVASS
                     PSLNNTRIRNTSSTLRTESHGLDVLGAVGLTVNNAEIEEYDYGVYWNGLGTTDYRSTP
                     SLNNTRIRNTSSTLRTVAKGVKMIDISRVTMTNDSIIGYPTGLEITNDGSGLLTTTAS
                     LNNTRIRNTSSTLRTETTGINFSGHILATMESLDIDEYSTGIYYEGNGMLSDRATVSL
                     NNTRIRNTSSTLRSVTKGLIMMNIGSVTITNDSIIGYPTGLEITNDGSGLLTTTASLN
                     NTRIRNTSSTLRTETKGINFTGHILASMDSLYLEDYATGIYYEGNGMPFNRTTPSLNN
                     TRIRNTSSTLREPLKGVVLKNLAGITLNKNIIYPKVQVGQGNLSGNGIFLQTVQNAQI
                     AHNTIWGLNTGLYATGSSNGTFTHNVIWTNGEQLNTPVILEDSNIPVSNSDISYAQGV
                     FPGTGNLNADPLFVAPDEGNFYLKPRSPLANQGIGAMPFEFEVLAGVRTYTMHPGWNM
                     MGVPYLTRETYSTPVSIFADDLSPFYVAPTYTSIVQLNPSAPPDTLGHIDMSYTGSYV
                     IPSLVRPGIGYWVRNPYATPATIDVYGLMDDGSYNVVLDGTESPNNGWYLLANPYDLP
                     IGLNNGIILGEGMLVGLLKYNYVTNSYYFIDSEHPEVIEPWSGFFVKARAQDAHLYLN
                     YPSQARQENENQPFVSDIPNVVSRPKLKWEFSLNAKAGEKGDAIILGVGEGAKDTLDP
                     LDIPEIPNPPFPQTGILQFSICNNDWENNAGLYTRDIKNSRDGSWSWNLLLNLENMLE
                     DGIFSDIIRISALGAEKIPAGYIFRIVNPADGSSVDLRNEDFLLNVEIFHAEGEPWLI
                     PLYLEVIPLANDSGEREVFSASNYPNPFNPETTISYNLPAESFVTLEIYNLKGQLVKR
                     LVSENKPSGAHKAVWNGADQQGREVASGFYFYRLKTKDNQLTRKILLMK"
     gene            4960..>5878
                     /locus_tag="PLG69_06370"
     CDS             4960..>5878
                     /locus_tag="PLG69_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HPI43170.1"
                     /translation="MNAYYRQPAIYKDQVVFIAEDDLWTFNLKDKVARRLTANLGMIR
                     YPQFSPDGKQIAFTSTEEGTSEIYIMPSEGGEAKRLTYLGAYPYTLRWKGSKIIFASQ
                     HSSPLQHYNLYEIDSEGGTPVELPYGRATDISFGEKGVVLGRNTGNPARWKRYRGGTA
                     GYLLVDKNGDGKFKKLIDLKGNFAHPMWIGNRIYFICDHEGIGNIYSCTPEGKDLQKH
                     SHHQDFYARGAYSDGKSIIWHAGADLWLMDIKENKPRKLSFAYSSPMVQRQHKFVSPP
                     KNLEDIYLSPDGGSISFALRGKALVSGNWE"
BASE COUNT         1833 a         1059 c         1219 g         1767 t
ORIGIN      
        1 ccgatggggg aactgaacat tatttggcag tattggattt acagtccaga accacagtgc
       61 aggttttgga tatttatcct cattatgctc aaggtgttac aataggtcct gatggcaagg
      121 tgctgataaa tgattatgaa aatggttatg tgcatcagta tattctgaat tttgtaaccg
      181 gcaatttatc ctattctggt gtttccattc ctgtagcgtc ctgtttgaat acagttatta
      241 gtcctggtgg gaattttgcc attgcctgtt cttataatag agatactaaa gtcctaaaac
      301 ttaatgctga taatactata gatgtaatac aaaacctggg attaagttct caatcggctg
      361 tttattcttc agatggacaa attgccttaa ttggagaata tagtagctcc aatgatatat
      421 tagcgaagta taatgtgcag ggaaatggta gcttggtttg gcagcagagt tttaatcttc
      481 ccttttattc ggagagtgga tattatggag tggatgtttt ggctttaagt acggataaca
      541 gtaaggcata tattactgac tttggagcag aagccaacaa cattttagtt aatatcaatc
      601 tggaaaatgg cactcagcaa aatttaaccc tgcctgatcc tacgggttta gctattggaa
      661 caatgcactt aacagcatat tttacagcta ccataactaa tactcaaaac tcaacccagg
      721 tgcaatttca tcaacactgc agtggaaatc ctgattccta tttgtggaat tttggagatg
      781 gacaaaccag cactgaaccc aatccttatc atatatatta cactccaggt ttatatactg
      841 taacgctaac tgtgtataaa aatggcgaaa gtgacagcta ttcacaaacc atagaggcaa
      901 tgttcaatac ccaaattcat cttaccttag atggctcacc ctattattat tcagctaccc
      961 ttgctatttc ggaagggagt tctcttataa ttgataatgg agtgacggta aacttcggtc
     1021 cgcaagcagg tttagaggtt tggggttcaa tttctgctaa tggagttact ttttccggaa
     1081 gtgaaacaga gggctggaaa ggaattgttt tgcattctcc cgtttctcct gtagctttca
     1141 ataactgcaa tatctataat gctgtagttg gcttaaagat tataaacagc agtttcacaa
     1201 ttaccaattt gctgattagt aaaacccatt cttttaccgg agaaattgga ctaaaagtgc
     1261 aaggcgcatc taatatggta ttaaataatg tgcaggtgat tggttacatt atgggcattg
     1321 tttttgaaaa tgaagcaaga gttgccagca gtccttctct gaataatacc cgcattcgca
     1381 ataccagcag tactcttaga acggaaagtc atggactgga tgttttggga gctgtgggct
     1441 taactgtaaa caatgcagaa attgaggaat acgattatgg tgtttactgg aatggtttag
     1501 gaacaaccga ttatagaagc actccttctt taaataatac caggattaga aatacgagca
     1561 gtactttgcg cactgttgcc aaaggtgtga aaatgattga tatcagcaga gtcactatga
     1621 caaatgattc cattatcggt tatcccaccg gcttggaaat taccaatgac ggttcaggac
     1681 ttttaactac tacagcttct ttgaataata cccgtattcg caataccagc agcaccttaa
     1741 gaactgaaac cacgggcata aactttagcg gtcatatttt agcgactatg gaatctctgg
     1801 atatagatga atattctacc ggaatttact atgaaggcaa tggaatgctt tctgatcgtg
     1861 caactgtttc tctaaataat accaggatta gaaatacgag tagtacactt cgttccgtaa
     1921 ctaaaggttt aataatgatg aatatcggta gtgtaactat aacaaacgat tccattatcg
     1981 gttatcccac cggcttggaa attaccaatg acggttccgg acttttaact actacagctt
     2041 ctttgaataa tacccgtatt cgcaatacca gcagcacttt aagaactgaa accaagggta
     2101 taaacttcac cggtcatatt ttggcttcaa tggattctct atatcttgag gattatgcca
     2161 caggaatata ttatgaagga aatggaatgc cttttaacag gacaactcca tctttaaata
     2221 atacccgcat tcgcaatacc agcagcactt tgagagaacc cttaaaggga gtagtattaa
     2281 aaaacctagc aggaataact ttaaataaaa atatcattta tccgaaagtg caggttggac
     2341 agggaaatct gagtggtaat ggtatttttc tgcaaacagt gcaaaacgcg caaatagctc
     2401 ataacacaat ttggggttta aatactggtt tatatgctac gggtagtagt aacggtacct
     2461 ttacgcataa tgttatctgg actaatggag aacaattaaa tactcctgtt atcctggaag
     2521 atagcaatat acctgttagc aatagtgata tttcttatgc tcagggtgta ttcccgggaa
     2581 cgggtaatct gaatgcagac cctctatttg tagcccctga tgaaggtaat ttctatttga
     2641 aaccgcgttc tcctcttgcc aatcaaggca ttggagctat gccttttgaa tttgaggtgt
     2701 tggcaggagt gcgaacttat actatgcatc ctggatggaa tatgatgggt gtcccatatt
     2761 tgacaagaga aacctatagc actcctgtaa gcatatttgc ggatgatctt tctccttttt
     2821 atgtggctcc aacttacact tccattgtgc aattaaatcc atctgctcca cctgatacct
     2881 taggtcatat tgatatgagt tacaccggta gctatgttat tccttctctt gtgcgtcccg
     2941 gaataggtta ttgggtacgc aatccttatg ccactcctgc aacgatagat gtttacggtt
     3001 taatggatga tggcagttat aatgttgttt tagatggaac cgagagtcct aataacggct
     3061 ggtatctttt agccaatcct tacgatcttc ctattggtct gaataatggt atcattttgg
     3121 gagagggaat gttggttggt ttgctaaagt ataattatgt gaccaatagt tattatttta
     3181 ttgattccga acatcccgag gttattgagc cgtggagtgg attttttgtg aaagccagag
     3241 ctcaggatgc tcatttatat ttaaattatc cttcacaagc gcgtcaggaa aatgaaaatc
     3301 agcccttcgt aagtgatatc cccaatgtag tttcccgtcc caaactgaaa tgggagtttt
     3361 ctcttaatgc caaagcagga gaaaaaggtg atgctattat tttaggcgtt ggcgaaggag
     3421 caaaagatac tcttgatcca ttagatattc ctgaaattcc caatcctcct ttcccgcaaa
     3481 ccggaatact gcaattcagt atttgcaata atgattggga aaataatgcc ggactttata
     3541 cccgagatat aaaaaacagc agggatggtt cctggagctg gaatttatta ctcaacctgg
     3601 aaaatatgct ggaagatgga atttttagtg atataatccg catcagtgct ttaggtgcag
     3661 aaaagattcc tgcgggttac attttccgca ttgtaaatcc tgccgatggt agctctgttg
     3721 atttaaggaa tgaggatttc ctgctgaatg tagaaatctt tcacgcagaa ggagaaccct
     3781 ggttaattcc tttatacctg gaagttattc ctctcgccaa tgatagtggt gaaagagaag
     3841 tattttctgc aagcaattat cccaatcctt tcaatccgga aactacaatc agttataacc
     3901 ttcctgccga aagctttgtt actttggaaa tttataacct gaaaggtcaa ttggtcaaga
     3961 gattagtaag cgaaaataaa ccttccggtg cacataaagc tgtttggaac ggagctgatc
     4021 aacagggtcg tgaagttgct tccggattct atttctaccg cttgaaaacg aaggataatc
     4081 agctgacaag aaaaatacta cttatgaaat gaagaatagt gaaggaaaaa aggactcacg
     4141 cagatttcgc agatgacgca gattaaaaat gagaataggt atgggatgaa caggataaaa
     4201 gggatttcca ggatttggag tattagttcc gtaggaatga cagatactaa agactggttt
     4261 caacccggtg ataagaaaag aacctaaaaa actatattta ttggggaaaa agttccgtag
     4321 gaacggcagg tactaacaac gggtttttaa cccggtgaga agtaaggagg agagttatta
     4381 tctctttacc cgacaaagag ttccgtagga acgacagata ctaacaatgg gtttttaacc
     4441 cggtgaaaag taagtaggag agtcattatc tcttttcccg agaaaaagtt ccggaggaac
     4501 gacagatact aacaacgggt ttttaacccg gtgatacgaa aagaacctac aaaactatat
     4561 ttattggggg aaaaagttcc gttggaacga cagatactat agaagggttt taacccggtg
     4621 aaaagaaacc agagtaaaaa aggttccgtg ggaacttttt catcttgttc caatagttat
     4681 tatgggataa aatataattt ttatatctaa ctaaataccc tttagcaaac tacaaagggg
     4741 agaagctttg aattatatta acattgcttt aatatgccaa ttattcaacg gtattatttt
     4801 atatgcaaga agttaattca agcaaaagaa tattatattt tttgtgtacc ttatcgggca
     4861 attatacttt taagcttcta ataaacttct tgactgcaat atcctttttc aaattgttaa
     4921 ctaaacagtc aaaattttat cagaaaaagg agcatcccta tgaatgcgta ttatcgtcaa
     4981 cccgcaatat ataaagatca ggtggtcttt attgctgaag atgacctctg gacttttaac
     5041 ttaaaagata aagttgccag gcgtctaact gccaatttgg gtatgattcg ctatcctcaa
     5101 ttttctcctg atggtaagca aattgctttt accagcacag aagaaggaac ttccgaaatt
     5161 tatataatgc cttctgaagg tggcgaagca aaacgcttaa cttatttggg agcttatcct
     5221 tatacgctta gatggaaagg cagcaaaatt attttcgctt ctcaacacag ttctcctctc
     5281 cagcattaca atctttacga aattgacagt gaaggtggaa ctccggttga actaccttat
     5341 ggaagagcaa ccgatatttc ttttggtgaa aaaggagttg ttttaggtag aaataccggc
     5401 aaccctgcaa ggtggaaaag atatagaggt ggaacagccg gttatttatt agtggataaa
     5461 aatggcgatg gcaaattcaa gaaactgatt gacctgaaag gaaattttgc tcatcctatg
     5521 tggattggta accggattta ctttatttgc gatcacgaag gcatcggcaa tatttattct
     5581 tgcactccgg aggggaaaga cctgcaaaaa cacagccatc atcaggattt ttatgcccgc
     5641 ggtgcctatt ccgatggcaa aagtattatc tggcacgcag gagcagacct ttggctgatg
     5701 gacattaaag aaaacaaacc ccgaaaattg tcttttgctt atagcagccc gatggtacaa
     5761 aggcaacaca aatttgtcag ccctcctaaa aatttggagg atatatatct ttctcccgat
     5821 ggaggttcca tctcctttgc cttgcgcgga aaagcattag tgagtggaaa ttgggaag
//
LOCUS       DAOPFL010000096         5853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_17939_length_5853_cov_106.423767, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000096 DAOPFL010000000
VERSION     DAOPFL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17939_length_5853_cov_106.423767"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLG69_06375"
     CDS             complement(<1..638)
                     /locus_tag="PLG69_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43171.1"
                     /translation="MKKILLTLVFTSVLLFILAVPRNLVVVEIATGTWCPYCPGAAMG
                     ADDLIDAGQPVAIVENHNGDTFANVYSNARNSYYNPSGVPTAYFDGLNAVVGGDHTQS
                     MYSYYLPRVTSRIAVPSKYTISATGNLTGNNLTVAATVAKPEADTNTNVLFHCVITQS
                     HIQYSWQGQTHLNFVNRLMLPNQNGTAVTLATGEQQTYNLTGIFNLEWDITS"
     gene            complement(676..1827)
                     /locus_tag="PLG69_06380"
     CDS             complement(676..1827)
                     /locus_tag="PLG69_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPI43172.1"
                     /translation="MNDKLTDIFNEIRASYESTLGKRAFHSANAWRSKKEEPDIMRSQ
                     FAVDRDRILYSGAYRRYHGKTQVFSFTNMIDEEMTNRNLHIAYVSQISRTIGKYLKLN
                     TELIEAIALGHDLGHCPFGHDGENALSQCCEKNGIGEFHHNIESLHIVDHISRKGKGL
                     NLTFQVRDGIASHDGEVHNTQLIPQTNKTEEDIQNYICAKKAGQDLQFMPATLEGCVV
                     RITDTIAYIGQDIEDAIRFNILTRDELPAEQVAYLGNTNSQMIETLIKSVIVNSYEQD
                     FIAFDKETSEHLLALKKFNYKRIYTDENVKKSNSIIYRTMPILFDIYLNDIQENNRES
                     KIFKHFLDHKFPEYLENFSPAEKVRDFIATMTDRYYNEEIKAYLLPWRG"
     gene            2057..2311
                     /locus_tag="PLG69_06385"
     CDS             2057..2311
                     /locus_tag="PLG69_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43173.1"
                     /translation="MLYTLPDGRKINLARVKSISKIREYGQTGQIIEEQFIGFAIHLK
                     NNETIEIKDKYHYSDWSEVKIRLSKLRDDIIAKWEQVQKK"
     gene            2531..4642
                     /gene="fusA"
                     /locus_tag="PLG69_06390"
     CDS             2531..4642
                     /gene="fusA"
                     /locus_tag="PLG69_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPI43174.1"
                     /translation="MDTDADSPLTKIRNIGIMAHIDAGKTTTTERILFYTGYLHKMGE
                     VHDGNTFTDWMEQERERGITITSATVTCYWNAYQINIIDTPGHVDFTAEVERSLRVLD
                     GAIGIFCAVGGVEPQSETVWNQANRYHVPRLAYINKMDRVGADYFHTIQMIRERLTPN
                     AYPVNMPMGREENFEGVIDLIAMQAYYFDPLTFGYEVKQTTIPEKYLESAKSLRDELL
                     EKVSEFSDELLMLFLEGEEIPVSLLKEAIRKGTISSQFVPVFCGSSLKNKGVQLLLDG
                     ICDFLPSPLDIGDTQGFEPVTHNPVNISPDPKGPLVALAFKVQIDKYIGRLVYIRLYS
                     GTLKKGGSFVNQTNGKKERVSRILQMMSNRKNDLDCLHAGDIGAIVGSRFLITGDTIT
                     DGNFEVLLSKMHFPDTVISMAIEPKTKADQENLDIALMRLEEEDPTFRVHTDKDTGQT
                     LISGMGELHLEVIVDRLRREFGVAVNQGNPQVSYKETITKEVEAEEVFQRELNGKGNY
                     AYVKFRLSPIALKDLPEDAKNIFVNKISEEKIPSEFWKPIEEAVFSALNDGPLISCNV
                     ERVRIELIDGDYNPVDSNELAYRIATGIAISKGLRDASPVIMEPIMLLTVIAPDEFVG
                     DILNDINAKRGKIEIMRRENEFKQEIVAEVPLSELFGYSTRIRSLSQGRAIYTLEFKK
                     YEICPVQIQNAILKRIRGYVE"
     gene            4699..5007
                     /locus_tag="PLG69_06395"
     CDS             4699..5007
                     /locus_tag="PLG69_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPI43175.1"
                     /translation="MNIELSLDGKVAKMKIDGILNSENAYLLQEKLTEVLNSDATLLE
                     LDLLDCRNISSTGIGKILLFYKDFITKGGEIEVVRSSNSVYELFSMLKLNQLFTVNLG
                     "
     gene            5009..>5853
                     /locus_tag="PLG69_06400"
     CDS             5009..>5853
                     /locus_tag="PLG69_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPI43176.1"
                     /translation="MKHRILVVDDSSSLVSILSELLKFNGFEVETALSGTEALHKIES
                     EDYDLVICDIEMPGMSGLEFLSRVRRDYEKDIAFILMTGYADNEYFIEAIRLGASDFI
                     HKPIDSKQMMRSIQTILKRKKKRSDFSEFYNNLEKAEFHFELDPRNFSKFAVSEVFHN
                     FLRQNFDLTPNVLNEILICIDEMVYNAYIHGTLGLNVQERVMEHNALQKIINERLQQP
                     EIASKRMRLYFSLCYKTQTIKITVEDDGPGFDYETWIKRVANEPKLNLEENGRGIAML
                     YHLSD"
BASE COUNT         1866 a          976 c         1204 g         1807 t
ORIGIN      
        1 caggaagtta tatcccattc aagattaaat attccggtaa gattataggt ctgctgttca
       61 ccagttgcaa gagttacagc tgttccgttt tgattgggca acattaaacg gtttacgaaa
      121 ttcaaatgtg tttgaccctg ccatgaatat tgaatatgtg attgggtaat aacacaatga
      181 aaaagcacat tagtattggt atccgcttcc ggtttggcaa ctgtagcagc aacagttaaa
      241 ttatttccag tcaagtttcc agttgccgaa atagtatatt tagaaggaac agcaatgcgg
      301 gaagttactc tgggaaggta ataagaatac attgattgag tatgatcacc acccaccaca
      361 gcatttaatc cgtcaaaata agcagtagga acgccactgg gattgtaata actatttcgg
      421 gcattagaat agacattggc aaaagtatcg ccgttatggt tttctacaat agctaccggt
      481 tgacctgcat caattaagtc atcggcaccc attgcggcac ctggacaata gggacaccag
      541 gttccggtag caatttccac tacaaccaaa tttcggggaa ctgccaaaat aaagagcaga
      601 acactggtaa aaacgagggt taagagaatt tttttcatta ttactcctta tgtttagtta
      661 attgaaaaac attgtttatc ctctccaggg gagtagatat gccttaattt cttcgttgta
      721 atagcggtct gtcatagttg ctataaaatc acgcactttt tcagccggcg agaaattttc
      781 caagtattca ggaaatttgt gatctaggaa atgtttgaat atcttagatt cccgattatt
      841 ttcctggatg tcatttaagt aaatatcaaa aaggataggc atagtgcggt aaataatgga
      901 atttgatttt ttcacatttt catcggtgta gattcttttg taattgaatt tcttcaaagc
      961 caaaagatgc tcactggtct ctttgtcaaa agcaatgaaa tcttgctcat agctatttac
     1021 aatcacgctt ttaatgaggg tttcaatcat ctgactatta gtattaccca aataggcaac
     1081 ctgctctgcg ggaagttcat cgcgagtaag aatattaaaa cgaatggcat cctctatatc
     1141 ctgtccaata taggcaattg tatcggtaat acgaactaca cacccttcca gagttgcagg
     1201 cataaattga agatcctgcc cagctttttt ggcacaaatg taattctgaa tatcctcttc
     1261 ggttttattt gtttggggaa ttaactgggt gttatgcact tctccgtcgt gggaagcgat
     1321 tccatctcgc acttgaaaag ttaaattcag tccttttccc ttgcgggaga tatgatccac
     1381 aatatgtaaa ctttcaatgt tatgatgaaa ttctccgata ccgttttttt cacaacattg
     1441 ggaaagagca ttttctccat cgtggccaaa aggacaatgc cccaaatcat gacctaaagc
     1501 tatcgcttca atcaactccg tgtttaactt caaatatttt cctatagtgc gcgagatttg
     1561 agaaacataa gcaatgtgca gattgcgatt ggtcatttct tcatcaatca tattggtgaa
     1621 agaaaaaacc tgtgtttttc catgatacct gcgataagcg ccactgtaaa ggattctatc
     1681 tctatccacc gcaaattggg aacgcataat atccggttct tcttttttgc tgcgccaggc
     1741 atttgccgaa tgaaatgcgc gtttgcctaa ggtgctttcg taggaagctc tgatttcgtt
     1801 gaagatatca gtcagtttat cattcattac tttctcccca ttactcttta atgatataat
     1861 attgcaaaaa ggtctgctgt caagaataac ttggaatctt tatcaccaac aaggagtaaa
     1921 aggattttta aggattcata gagattttca ttttttccat tcttaattag aatgtaaaag
     1981 ggctaaaata aatattgaca aaatgatttg atacaataat atgactttta gtgagattca
     2041 taggaaggtt ataaaaatgc tttatacatt acccgatggc agaaaaatta atttagcaag
     2101 ggtaaaaagt atctctaaaa tcagagaata tgggcaaaca ggtcagatta ttgaagagca
     2161 atttatcgga tttgctatac atttgaagaa taatgaaaca attgaaatta aagataaata
     2221 ccattatagt gattggtcgg aagttaagat acgactgagt aaattgcgag atgatataat
     2281 agctaaatgg gagcaagtcc agaagaaata accgtgtttg ttttgtatta atcgggtggt
     2341 aaatttattt ttgctgctga gcatttagtt ctcttcaata attattcctt ttgcctttac
     2401 ccaatggtga tttttccacc taatacttta tcccttttgg cttgttaacc ttcagagcac
     2461 ttggactttt ttatagacaa ataagctaac cctgtttttt gggttaataa attaaaaaaa
     2521 ggatttattt atggataccg atgcggatag tccgcttact aaaatacgca atattggtat
     2581 aatggctcat attgatgccg ggaaaacaac aactacagaa cggatattgt tttatacagg
     2641 ttatctgcat aaaatgggtg aagtgcatga tggaaataca tttacggatt ggatggaaca
     2701 ggaaagggaa agaggaatta caatcacttc tgcaacagta acttgttatt ggaatgccta
     2761 tcaaataaat attattgata ctccgggtca tgtagatttt acagcagaag tagaacgttc
     2821 cttacgggtt ttggatggag caataggtat attttgtgca gttggaggtg tggaaccaca
     2881 atcagaaact gtatggaatc aggcaaatcg ttatcatgtt cctcgtttag cgtacataaa
     2941 caaaatggat cgggtaggcg cagattattt tcataccata caaatgatta gagaacggct
     3001 tactcccaat gcttatcctg taaatatgcc aatgggcagg gaagaaaact ttgaaggtgt
     3061 aattgattta atcgcgatgc aggcatatta ttttgatcct ttaacctttg gctatgaagt
     3121 gaaacagaca actattccgg aaaaatatct ggaaagcgca aaaagtttga gggatgagct
     3181 tttagaaaag gttagcgaat tcagtgacga attactgatg ttgtttttag agggagaaga
     3241 aattcctgta agtttattaa aagaggcaat tcgcaaaggg acaatcagca gtcaatttgt
     3301 cccggttttt tgtggcagtt ccttaaaaaa taaaggggtt cagcttcttt tggacggaat
     3361 ttgtgatttt ctgccttcac ctcttgacat tggagataca caaggttttg aacccgtaac
     3421 acataatcct gttaatatta gtcctgatcc caaaggacct ctggtagctt tagctttcaa
     3481 agtgcaaata gataaatata ttggacgcct tgtttatatc aggctttatt ccggaacctt
     3541 gaaaaaaggc ggttcctttg taaatcagac gaatgggaag aaagaaagag tttcgcgtat
     3601 tctgcaaatg atgagtaacc ggaaaaacga tttggattgc cttcatgcag gtgatattgg
     3661 agctattgta ggttccagat ttctaataac gggagataca attaccgatg gtaactttga
     3721 agttttgctt tccaaaatgc attttccgga tactgtaatt tctatggcta ttgaacccaa
     3781 aaccaaagcg gatcaggaaa atctggatat tgccctaatg cgtttggaag aagaagaccc
     3841 tactttccgt gtgcataccg ataaagatac gggacagaca ttgatttcag gaatgggcga
     3901 attgcatttg gaagttattg tagatcgttt gcgtcgggaa tttggcgttg cagtaaatca
     3961 gggtaatccc caggtttcct ataaagaaac gattactaaa gaagtagaag cagaagaagt
     4021 tttccagcgt gaactgaatg gcaagggaaa ttatgcttat gtaaaattcc gcttaagtcc
     4081 aattgcttta aaagacctgc cggaggatgc caaaaacatc tttgtaaata aaataagtga
     4141 agaaaaaatc ccgtcggaat tttggaaacc tattgaagaa gctgttttca gtgctttgaa
     4201 tgatggacct ctcattagtt gtaatgtaga aagagtgcgt atagaactta ttgacgggga
     4261 ttataaccct gtggattcta atgaacttgc ttatcgcatt gcaaccggaa tagctatcag
     4321 taaagggctt agagatgcct ctccggtaat tatggaacct attatgttgc ttacagtaat
     4381 agctcctgat gaatttgtag gtgatatttt aaatgatata aatgccaaac gggggaagat
     4441 agaaattatg cgcagggaaa atgaatttaa gcaggaaatt gtagcagaag ttcctctttc
     4501 cgaacttttt ggttattcaa cacgcattcg ttctttaagc caaggcagag ctatatatac
     4561 tttggaattt aagaaatacg agatttgtcc ggtgcagatt caaaatgcca ttctgaaaag
     4621 aattcgcggt tatgtagaat aaatccttga cagacaaaag gatattttag ataaagaaaa
     4681 caaaaggagg ttataaagat gaacattgaa ctttcccttg atggcaaggt cgctaagatg
     4741 aagattgatg gtatcttaaa tagcgaaaat gcctatttgc ttcaggaaaa attaactgaa
     4801 gttcttaatt ctgatgccac tcttttggaa cttgatttgc tggattgccg gaatataagt
     4861 tctacaggga ttggaaaaat tttgttgttt tacaaggatt ttattaccaa aggaggagaa
     4921 attgaggtgg ttagaagttc caatagcgta tatgagttat tttctatgct caaactaaat
     4981 caattattta cagttaatct gggctgaaat gaaacaccgc attcttgtag tggatgacag
     5041 tagttctctt gtttccatct tgtccgagtt gttaaaattt aatggttttg aagtagaaac
     5101 agctctcagt ggaacggaag cattgcataa aattgaaagc gaggattatg atctcgtaat
     5161 ttgtgatatt gaaatgccgg gaatgagtgg tctggaattc ctttcccgcg tgcgcaggga
     5221 ttatgaaaaa gatatagctt ttatcttaat gacaggttat gcggataatg aatatttcat
     5281 tgaggcaatc cgacttggcg cttcggattt tattcataaa cccatagaca gtaaacaaat
     5341 gatgcgttcc attcagacca ttctgaaacg caagaaaaaa cggagcgatt tcagcgagtt
     5401 ttataacaac ctggaaaaag cggagtttca ttttgaactt gatccgcgga atttctccaa
     5461 atttgctgtt tcggaggtct ttcacaactt tctgcgccag aattttgacc tcacccccaa
     5521 tgtgttaaac gaaattctga tttgtataga tgaaatggtt tacaatgctt atattcatgg
     5581 aactctgggc ttaaatgttc aggaacgggt tatggaacat aatgccctgc agaaaataat
     5641 aaatgaacgc ctgcaacaac ctgaaattgc ctctaaaaga atgcgccttt atttttctct
     5701 ttgttataaa acacaaacaa tcaaaataac cgttgaagat gacggacccg gctttgatta
     5761 tgaaacctgg ataaaaaggg ttgccaatga gcccaaactg aatctggaag aaaatggcag
     5821 aggaattgcg atgctttatc atctttccga caa
//
LOCUS       DAOPFL010000097         5762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_18343_length_5762_cov_105.022954, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000097 DAOPFL010000000
VERSION     DAOPFL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18343_length_5762_cov_105.022954"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1178
                     /locus_tag="PLG69_06405"
     CDS             321..1178
                     /locus_tag="PLG69_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HPI43177.1"
                     /translation="MQRKNNYTLFRIGELVPSGTYTKVHSCFERVINFAAEDIVISLC
                     ESSICAGPYRLILKGKIPLAKELKVTEKEVVINQEISLPYSQDNIWKIPSIHPYKNAQ
                     KTFLLCEKLLNFFMGEEKNNLLSLLFQPPSSQDEFLLQFRNFLIKGINNLFQQKYTLF
                     AQQIKRRGLGSTPAGDDFLTGYIICLSWLIANGRKDLIKQREEIYETALDNDLLVNTF
                     LHQAYYLRPDEEWANFLLALSENDEINLTKYINLIMNSGESSGKDMLSGFVFCLLFNW
                     FWIAEIAKC"
     gene            1246..2763
                     /gene="fdrA"
                     /locus_tag="PLG69_06410"
     CDS             1246..2763
                     /gene="fdrA"
                     /locus_tag="PLG69_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HPI43178.1"
                     /translation="MPIKAKIIAGKYLDSVKLMLISRELRSLKGVLEAVAIVATSENR
                     EILKASDMLAEEINSASENDIVIVIKADNAKNAAEAINKAEELINKPSADEYNQTPIP
                     QAFTLEKAIATLGGADLCLISIAGKYAAAEAEKALEKGLHILLFSDNVSIEDELSLKQ
                     KALKKGLLMMGPDCGTAILNGIPLAFANNVPRGKIGIVSASGTGLQEISSTIANLGGG
                     ISQAFGTGGRDGKKEIGGLMLTACLEYLLEDENTEVIIVIGKPPCEEVQKKLWELISH
                     SSKPVIANFLIPMQVPELPNLYYVRSLSETAFFACKKAGIEVKTAKLAEDFPLPKLRG
                     PYLRGLYSGGTLCHEAIQVYLDNFGCEPFNNVTDQAFFRLTDGWQTKENSIIDLGADE
                     FTIGRPHPMIDYSLRLQKLAQEANDPKVGIILLDVVLGFGAHPNPAEELVPALQKLPA
                     DLVVVCHLLGTDGDPQNRQKQKKELEQAGAYVFISHHSACEFVCQCLRLYRSAKC"
     gene            2757..4154
                     /locus_tag="PLG69_06415"
     CDS             2757..4154
                     /locus_tag="PLG69_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HPI43179.1"
                     /translation="MLKLNKDKPKVINIGLEKFAVDLKTQGIDVVNLLWKPPLPVENS
                     YHHKINSLPENIIAPANKKALEIINSGKAVLTGMDVALKVIPGMHKDLILHSGPPISW
                     DRMCGPMRGAIIGALIYEDRAKNAEEAEKLAASGKIEFAPCHEHNCTGPMAGIISPSM
                     PVFIIKNESYGNFTYATQNEGLGKVLRYGAYSEEVISRLKWMEKVLYPVLKRAIEYAG
                     TINIQGLIAQALNMGDELHNRNRAGTSLFIRQIAPAIIKTMTNPEESAAALDFINSND
                     HFFLNLSMPASKAILDPARNISGSSIVVAMARNGTDFGIQVSGTGNKWFTGTAPVPDA
                     LYFPGFTKEDANPDIGDSSITETGGLGGFALAAAPAIVQFVGGTAKEAINYTLSMYNI
                     CFGESSLYQIPALDFRGTPLGIDVVKVIQNNIAPVIDTGVAHKNPGVGQVGAGVVKAP
                     FEPFIKAYKTLADMY"
     gene            4326..5138
                     /locus_tag="PLG69_06420"
     CDS             4326..5138
                     /locus_tag="PLG69_06420"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPI43180.1"
                     /translation="MELNSFFEFMQSLKMYDLTQPLSIHTPPWPSYMPLGIQYFKRIA
                     GAHSGQGANGQIITTSNHVGTHIDGEIHFFASGRSIGQVPMEEWIGPGVVVDISDSVN
                     DYDLYSPELLMQKAEIKKGDILIINTGYHRYAWDQPESDELRYFVKHPGPDPSFHTWA
                     LDMHLKWIGVDCGSADHPMNTIIRNWHPGSFNEAEQKLIAKYSKTWDEMFPPEVYYQV
                     MHLKLFPKKLVHAENLGGDIDKISNKRVWIGLFPLRGIELESSMCRIMAWEP"
     gene            5153..>5762
                     /locus_tag="PLG69_06425"
     CDS             5153..>5762
                     /locus_tag="PLG69_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPI43181.1"
                     /translation="MALAHTFDYQRPSTLIEALQLKAKYKDKAHFLAGGTDLIVNIRE
                     GMLKPDLLIDIKGISELLKLNWEKETLFIGSNVTFAQLIDNELVQKHFILLHDACKTI
                     GSTGIRARATLTGNICSAVPSLDSAPALLCYEAKVHCASPESQREIPVTEWFLAPRKT
                     ALQEDEIVTGISLKRIPDSSGIYLKLGRYSGEDLAQAGWGILM"
BASE COUNT         1790 a         1068 c         1193 g         1711 t
ORIGIN      
        1 ataatttacc taaaaacaac tcagccatat aatcttgcca aattacaact catcctccaa
       61 ccatccttta ctcatcctcc aaccatcctt tacccatcct ctatccatcc atccatcagg
      121 ttgggttaat gatggctaac aagaaaggac ggctctcaca gattacacag attgcacgga
      181 ttttattgat ttgcgagtat tttttattac acgatttttg tagccggagc tcgctgtagc
      241 tccgcaattt ttcttacaaa aaaacagtgt tctccatcta ccaatttgac atttaattac
      301 aaaagcatta ttttattgtt atgcagagaa aaaataatta tacccttttt cgcatcggag
      361 aattggttcc ttcaggcacc tatacaaagg ttcatagctg ttttgagcgg gtaataaatt
      421 ttgccgcaga agatattgtt atatctttat gtgagagtag tatatgtgca ggtccatatc
      481 gtctaatcct aaaaggaaag attcctttag ctaaagaact gaaagtaaca gaaaaagaag
      541 tggttattaa tcaagaaata tctttaccat acagccagga taatatctgg aaaatacctt
      601 caattcatcc ttataaaaat gcccaaaaga ccttcttact ctgcgaaaaa ctgcttaatt
      661 tctttatggg tgaagagaaa aataatttat tatccttgct ttttcagccc ccttcatccc
      721 aggatgaatt tcttcttcag ttccgtaact tcctcataaa aggtataaat aacctctttc
      781 aacaaaaata tacactcttt gcccaacaaa taaaacgccg gggtttggga agcactcctg
      841 ccggtgatga ttttttgact ggctatataa tctgtttatc ctggctgatt gccaacggca
      901 gaaaagacct gataaaacaa cgggaagaaa tctatgaaac cgctttggac aatgatttgc
      961 tggtcaatac ttttctgcat caggcatatt acctgcgtcc ggatgaagag tgggcgaatt
     1021 ttttgcttgc tttaagtgaa aacgacgaaa ttaacttgac aaaatacata aatctaataa
     1081 tgaattcagg tgagagttcg ggaaaagata tgttgagcgg atttgtattt tgtttgttat
     1141 ttaattggtt ttggattgcg gaaatagcga aatgctgaag ttgtattttt ccggatgtcc
     1201 ttgctgctct caatgtgcta tctgtaaata tataaaggaa atataatgcc cataaaagct
     1261 aaaattattg cgggaaaata cttggattct gttaagctga tgcttatttc ccgggaacta
     1321 aggtctctaa aaggcgttct ggaagcagtt gctattgtag ctacaagcga aaatcgggaa
     1381 attctaaagg caagtgatat gctggcagaa gaaataaact ctgcttcgga aaacgatatt
     1441 gttatcgtga tcaaagccga taacgcaaag aatgctgcgg aagcaataaa taaagcagaa
     1501 gaattaatca ataaaccttc cgctgatgaa tacaatcaaa ctccaattcc ccaggcattt
     1561 actctggaaa aagcaattgc aactttagga ggagcagacc tttgcctaat ttccattgcc
     1621 ggaaaatatg cagccgctga agcggaaaaa gcactggaaa aaggactgca tattttgctc
     1681 ttttccgata atgtaagcat tgaagatgaa ctttctttga aacaaaaagc tctgaaaaaa
     1741 ggtcttttga tgatgggtcc tgattgcgga accgctattc tcaatggcat tccacttgcc
     1801 tttgccaata atgttccccg cggtaaaata ggaattgttt ccgcatcagg aacaggttta
     1861 caggaaatct cctctaccat cgctaatttg ggtggaggaa tttcgcaggc atttggaacc
     1921 ggaggaaggg atgggaaaaa agaaattggc ggcttaatgc tgactgcctg tttggaatat
     1981 ctgctggagg atgaaaatac agaagttatt attgtaatag gaaaaccccc ctgtgaagag
     2041 gtgcagaaaa aattgtggga actgatttct catagttcca aaccggtgat tgccaatttc
     2101 cttattccaa tgcaggttcc tgaactaccc aatttatatt atgtccgttc tttgtctgaa
     2161 accgcttttt ttgcctgtaa gaaagcagga attgaagtaa aaactgctaa gcttgcggaa
     2221 gattttcctt tgccaaagct aagaggtcct tatctgcgcg gtttatatag tggcggaacg
     2281 ctttgtcatg aagcaattca ggtctatctt gataattttg gttgcgaacc ctttaataat
     2341 gttacggatc aggcattttt ccggttgaca gatggctggc aaacaaaaga aaatagtatt
     2401 atagatttag gagcagatga atttacaata ggacgtcctc atccaatgat agattacagt
     2461 ttgcgtctgc aaaaattggc tcaggaagca aatgatccta aagtaggtat catcttattg
     2521 gatgtagttt tgggttttgg tgctcatcct aatccggctg aagaacttgt tcctgccctg
     2581 caaaaattgc ctgcagacct tgttgttgtc tgccatttat taggaacaga cggcgatcct
     2641 caaaaccggc aaaaacaaaa aaaggaattg gaacaggcag gagcttatgt atttatctct
     2701 catcattctg cttgtgaatt tgtctgccaa tgtttaagat tatatcggag tgctaaatgc
     2761 tgaaattaaa taaagataaa ccaaaagtaa taaacattgg cttggaaaaa tttgccgtag
     2821 accttaaaac tcaaggtatt gatgtggtaa acctcctttg gaaaccccct ctacccgttg
     2881 aaaacagcta tcatcataaa ataaattcgc tccctgaaaa catcattgct ccagctaata
     2941 aaaaagcgct ggaaataatc aattccggca aagcggtttt aaccggaatg gatgttgccc
     3001 tgaaggttat tcccggaatg cataaagacc ttattctcca tagcggtcct cctatttcct
     3061 gggatagaat gtgcggtcct atgcggggtg ccataattgg agctctaatt tatgaagata
     3121 gggcaaaaaa tgcagaagaa gcagaaaaac ttgccgcttc gggaaaaatt gaatttgctc
     3181 cctgccacga acataattgt accggtccta tggcaggtat tatcagtcct tcaatgcctg
     3241 tttttattat taaaaatgaa agctacggca attttactta tgctacccaa aatgaaggtt
     3301 tgggaaaagt tttgcgctat ggtgcctata gcgaagaagt gattagccgc ttaaaatgga
     3361 tggaaaaagt gctctatccc gtcctgaaaa gagctataga atatgcggga acgattaata
     3421 ttcagggact tattgcccaa gcattgaata tgggtgatga acttcacaat cgtaatcggg
     3481 ctggaacttc tttgtttatc agacaaatag ctcctgccat tattaaaaca atgactaatc
     3541 ctgaagaatc tgctgccgca ctggatttta taaatagcaa cgaccatttt ttcctgaacc
     3601 tgtctatgcc tgcaagtaaa gccattcttg atcctgccag aaacatttcc ggtagctcta
     3661 ttgttgtggc tatggcaaga aatggaactg acttcggaat tcaggttagc ggaacaggaa
     3721 ataaatggtt tacgggtact gcccctgttc ccgatgccct ctattttcca ggttttacca
     3781 aagaagatgc caatcccgat attggtgaca gctctataac agaaacaggc ggtttgggtg
     3841 gttttgctct tgccgcagct cctgccatag ttcaatttgt aggtggaact gccaaagaag
     3901 caataaatta cacattgtct atgtataata tttgttttgg agaaagctcc ctttatcaaa
     3961 ttccggcatt ggattttaga ggaacccctt tgggaattga tgtagtgaaa gttattcaaa
     4021 ataacatagc tccggtaatt gacacaggtg tggcacataa aaatcctggt gtgggacaag
     4081 ttggcgctgg agttgtgaaa gcaccttttg aaccgtttat taaggcctat aaaacattag
     4141 cggatatgta ttaaaaaaaa gataaagagt gagatagtaa gatgggaaaa tagtgagatg
     4201 gtgaaaatag tgaggaagcg aaatggtttt taggaaaaaa gatatctttg ttataatgaa
     4261 ctttgaactc ccgcactttg aactcccgca ctttgaactt tgaactatat aaacaggaga
     4321 taaaaatgga attaaacagc ttctttgaat ttatgcagag cttaaagatg tatgatttaa
     4381 ctcaaccctt aagtattcat acacctccct ggcccagcta tatgcctttg ggaattcaat
     4441 actttaaacg cattgccgga gctcattcag gtcagggtgc aaatggtcaa attattacta
     4501 ccagcaatca tgtaggcaca catattgatg gtgaaatcca tttttttgcc agtggacgca
     4561 gtataggaca agttcctatg gaagaatgga ttggtcctgg agtagttgta gatatttctg
     4621 atagcgtaaa tgactatgat ttatactctc ccgagcttct gatgcaaaaa gcggaaatca
     4681 aaaaaggcga tattttaatt atcaataccg gctatcatcg ttatgcctgg gatcaacctg
     4741 aaagtgatga actccgctat tttgttaagc atccaggtcc tgatccttct tttcatacct
     4801 gggctttgga tatgcatttg aaatggattg gagtagattg cggttctgca gatcatccta
     4861 tgaatactat tattcgcaat tggcatcctg gttcttttaa cgaagcggaa caaaagttga
     4921 ttgccaaata cagtaagacc tgggatgaaa tgtttcctcc tgaagtatac tatcaggtga
     4981 tgcatctgaa actcttccct aaaaaactgg tgcatgcaga aaacctgggg ggagatattg
     5041 ataaaatctc caataaaagg gtctggatag gactttttcc tctgcgcggc attgaactgg
     5101 aatcctctat gtgtcgcatt atggcttggg aaccctaaaa aaggaagaaa atatggcttt
     5161 ggctcatact tttgactatc agcgtccttc cactttgatt gaagcattgc aacttaaagc
     5221 aaaatataaa gataaagccc atttcctggc aggtggaacc gatctgattg taaatattag
     5281 agaaggaatg ctgaaacctg atttgttgat tgatataaaa ggtatcagtg aattattaaa
     5341 gctcaattgg gaaaaggaaa cactttttat cggcagtaat gtaacttttg cgcagcttat
     5401 agataatgaa ctggtgcaga agcattttat tcttcttcac gatgcctgta aaacaattgg
     5461 ctctacagga attcgtgccc gggctacttt aacgggaaat atttgttctg cagttccatc
     5521 tttggattct gctcctgctc ttttatgcta tgaggcaaaa gttcattgtg caagtcctga
     5581 gtcgcaaagg gaaattcctg ttacagaatg gtttcttgct ccccgaaaaa cagctttgca
     5641 ggaagatgaa atagtaaccg gtatttccct gaaaagaatt cctgatagtt ccggtattta
     5701 tttaaagctg ggtcgttaca gtggagaaga ccttgctcag gcaggttggg gaattttaat
     5761 ga
//
LOCUS       DAOPFL010000098         5519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_19482_length_5519_cov_116.310029, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000098 DAOPFL010000000
VERSION     DAOPFL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19482_length_5519_cov_116.310029"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLG69_06430"
     CDS             <1..208
                     /locus_tag="PLG69_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43182.1"
                     /translation="RTERYPVHIFEIRQIQLPTQQILKISTYNLPRPNPDVSGTSSVH
                     FRWFEPLVAGAAIGSLIFLLWNFD"
     gene            224..958
                     /gene="bamD"
                     /locus_tag="PLG69_06435"
     CDS             224..958
                     /gene="bamD"
                     /locus_tag="PLG69_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPI43183.1"
                     /translation="MRKYIFLLLITVFCLVSCSSNKTQLSTEAKLKNADELFAKKKYS
                     RAAVIYEEISFERKSAATAYATMKVADCYFNMNKFSDARAKYQQFINSFPDHENVADA
                     YFRIGVCLFEESLPPQYDQTETIKCIEAFQTFIDRYPNDPRYVQAVDYIHKCQYKLLE
                     KQYLTGYIYYKMKDYSSALMYFDEIVSLGNNDKLDRQSLYYSAKLHLHQKNYDKAKAS
                     YERLKNRYPDSKEAKSLAKKFAKLEK"
     gene            961..1542
                     /gene="nadD"
                     /locus_tag="PLG69_06440"
     CDS             961..1542
                     /gene="nadD"
                     /locus_tag="PLG69_06440"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPI43184.1"
                     /translation="MQGKVAVLGGSFDPVHSGHLHIANQILQQKAAETVLFVPSGHHH
                     FKKNSIILPFEKRYALVEKAIKNNPQFAISDADQEGSGYTAHLMQKLKRRYPDVDFSF
                     VIGSDNLKELHLWYDYPYLAKELHFLILPRPGYALLPEVINQLKATVLNIELCPVSAT
                     EIRQRIKNRESIKGMVPEELEQEIIQLYRETGI"
     gene            1898..3745
                     /locus_tag="PLG69_06445"
     CDS             1898..3745
                     /locus_tag="PLG69_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43185.1"
                     /translation="MMRYKVLILAVLLLSGAFLNSGISRYYTFAYTTAEYNPINGIQL
                     SFPDMNNYISSSIEIGFPFPYCGQIYEDVKISTNGFINPGANWNDDSPNNQLHYGIYP
                     IIAPLWDDLSLAEGSLQYATIGTAPNREFWVQYSNARWLYNNFNCYVNFQVVLSENGK
                     IEFRYGNTNGSPLNCSASIGINMPNAGAGNFFSITPGNPPTVSTTQDNYTINEIIPAG
                     TVYIFNPLPPVLYDLSLKIEYSMFYTEGIYHNLHESFPLPVTVKNNGTETVDSYDVVL
                     YCGLTELCRLNGISLAPADSHLYDLVATIDSCGWKHLTAIAELPEDEYPEDNIGDYSC
                     IIRPNPNTGIIVGSGDELQRIPIDVYWKYSLYETIYYAEEIGNSGTIYGISYYMNSTF
                     SHNLLVKIWVGTTNLTNLNEGWISAGELTPVFNSYIIMPPESNEVYFPFSTPYQYQQG
                     NLCVMVYHSHTYALACTDPFLAQTVPEMRALKRWTDAYDPDPYNPPVYNELNGKMPKT
                     AFYLIPEIPVQDEYLPQAKTIGCYPNPFKETTTLQLNLDKKQEVCLEIYNIKGQKVQT
                     LFNGLAEKGETEISWNGLDNNNRTVANGLYFCKLKTKDRCETAKLIVLK"
     gene            3888..4298
                     /locus_tag="PLG69_06450"
     CDS             3888..4298
                     /locus_tag="PLG69_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPI43186.1"
                     /translation="MDIKEILSQIRSIANNALGGGYSFGQPSKLGDLYVIPVARVTYG
                     MGGGADESSPDTAKKEEEDSSSPAEEKGKKVKNKVGFSGGGGIGIHSVPVGLFSLKEE
                     RIKFHPVIGFWEIIAMMSLSALFVLKMKKLRYKK"
     gene            4394..5374
                     /locus_tag="PLG69_06455"
     CDS             4394..5374
                     /locus_tag="PLG69_06455"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPI43187.1"
                     /translation="MLSIFSPSNYAPFNIACEEYILKNFPEDVFLLYINNPSIIVGKH
                     QNTLAEINYEWVREHNIPVVRRLTGGGSVFHDPGNLNYSFLMNESEDFNRNFERYTKP
                     ILAVLQELGIPAILEGRNDLTINGCKFSGNAKTNAFGKTLQHGTILFSSNISDLTAAL
                     KPREEKFNDKAVKSVQARVTNVSDHLPYPLSLQDFVTLVRAKVRTMYPDIQDYTFSLR
                     DKEEIQALMNSKYDTWEWNFGKSPRYNLCHSLKTKVGIIEFYLLVNKGIIEEVNIYGD
                     FFTNREISELENALCGIEHKPEKVAEVLKDMEYKSFFGEVKLEEIVKAMF"
BASE COUNT         1734 a         1018 c         1086 g         1681 t
ORIGIN      
        1 ccgcactgaa cggtatcctg tccacatttt tgaaattagg cagattcaac ttcctactca
       61 gcagattcta aaaatttcta cctataattt accgcgtccc aatccggatg tttcaggaac
      121 ttcctctgtt cattttcgtt ggtttgagcc cttagttgca ggtgcagcta ttggttccct
      181 catatttctc ctctggaatt ttgattaaaa aaaggaatat attgtgcgaa aatacatctt
      241 tcttttgctg attaccgttt tttgccttgt ttcctgcagc agtaataaaa cacagctttc
      301 tacagaagcc aagctgaaaa atgccgatga gctttttgct aaaaagaaat attcccgtgc
      361 tgcagttatc tatgaagaga tctcttttga acgcaaatca gctgcaactg cttatgctac
      421 aatgaaagtt gccgattgtt atttcaatat gaataaattt agtgatgcgc gagccaaata
      481 tcagcagttt ataaattcat ttcctgatca cgaaaatgtt gccgatgcct attttcggat
      541 aggagtatgc ctgtttgaag aatccctgcc tccgcagtat gatcaaaccg agaccataaa
      601 atgtatagaa gcatttcaga ccttcataga ccgctatcct aatgatcctc gctatgttca
      661 ggcagtggat tatattcata aatgccaata taagcttttg gaaaaacaat acttaaccgg
      721 ttatatctac tataaaatga aggactatag cagcgctttg atgtattttg acgaaattgt
      781 atctttaggc aataatgata aattagaccg tcaatcactc tactattccg ccaaactgca
      841 tcttcatcaa aagaattacg acaaagctaa agcgagctat gaacgcctga aaaatcgcta
      901 tcccgattcc aaagaggcaa aaagtttagc caaaaaattc gccaagctgg aaaagtaaaa
      961 gtgcagggta aggttgcagt tttggggggc agttttgatc ctgttcattc aggtcatctg
     1021 catattgcca atcaaatcct gcaacaaaaa gctgcagaaa cagttctttt tgtcccaagc
     1081 ggtcatcacc attttaagaa aaactctatt attcttccct ttgaaaaacg ctatgcctta
     1141 gtggaaaaag caattaaaaa caatcctcaa tttgctattt ccgatgctga tcaggaaggt
     1201 agcggttata cggctcattt aatgcaaaaa ctgaaaagac ggtatccgga tgtggatttc
     1261 agctttgtaa tcggttcgga caatctgaaa gagctgcatt tgtggtatga ttatccttat
     1321 ttggctaagg aattgcattt tttaatttta ccgcgcccgg gttatgccct tctgccggaa
     1381 gttattaatc agctgaaagc aacggtgtta aatattgaac tttgccctgt ttccgccact
     1441 gaaatcagac agcgcataaa aaacagggaa agcattaaag gtatggtgcc ggaagaactg
     1501 gaacaggaaa taattcagct ttacagagaa acgggaattt aagcccgcat atttattaac
     1561 aacacggatt ttacggatta aaaggattaa cccggatggt aatttttaat tctcaatttg
     1621 caattctcag ttctgcattt ttgtatctgg tttcccatta tgaacctgtt tttctcttgc
     1681 tatcttgcca tattgttttt ttctattttt tgctttggtc ttcctctctt gtttcccatc
     1741 atcaacccaa cctttaggat gagtggaggt tgagaggagg atgggtaata aacggaaagg
     1801 aattataaaa taaaaagctt gacggaaatt agtaaagaaa atatagataa aaatgggtaa
     1861 tgtatagtgc attaacttct aaaaaaggag gttagctatg atgcgctata aggttttgat
     1921 tcttgccgtt ctacttttaa gcggtgcatt tttaaacagc ggcatttctc gctattacac
     1981 ttttgcctac acaactgctg aatacaatcc tataaatggg attcaacttt cttttccgga
     2041 tatgaataat tacattagtt caagcataga aattggtttt ccctttcctt attgcgggca
     2101 gatatatgaa gatgttaaaa tatccactaa tggttttatc aatcctggtg ccaactggaa
     2161 tgatgacagc cccaataatc agttacatta tggcatttat ccgataattg ctcctttatg
     2221 ggatgatctt agtttggcgg aaggaagttt gcaatatgca accattggca cagctcccaa
     2281 ccgggaattt tgggtgcaat actccaatgc tcgctggctc tataataatt ttaattgtta
     2341 tgtaaacttt caagtggtgc tttccgaaaa cggcaaaatt gaattccgct atggaaacac
     2401 taatggctct ccgctaaatt gttctgcttc tatcggaatt aatatgccca atgcaggagc
     2461 aggcaatttt ttcagtatca ctcccggaaa ccctcctacg gtatctacta cgcaggataa
     2521 ctatactata aacgaaatta ttcccgctgg aacggtttac atttttaatc ctttaccgcc
     2581 tgtgctttat gatctttctc tgaagataga atatagtatg ttttatacag agggaattta
     2641 tcataatctg catgaaagtt ttcctcttcc agtgacggta aaaaacaatg ggaccgagac
     2701 agttgatagc tatgatgtgg ttctttattg tgggttaacg gaactttgcc gtttaaatgg
     2761 catttccctg gcaccggcag acagccattt atatgattta gtggcaacta tagattcctg
     2821 cggctggaaa catttaactg caatagcaga acttcccgaa gatgaatatc cggaggacaa
     2881 tattggagat tatagctgca ttatacgtcc caatcccaat accggtatca ttgttggttc
     2941 aggagatgaa ctgcaaagaa ttcctataga tgtttactgg aaatattccc tttacgagac
     3001 aatttactat gctgaggaaa taggtaatag cggaactatt tatggaatta gttattatat
     3061 gaattccact ttttctcaca atctattggt aaaaatctgg gtagggacaa ccaatttaac
     3121 taacttaaat gaaggctgga tttctgcagg cgaattgaca cctgtcttta attcctatat
     3181 tattatgcca cccgaaagta atgaagtgta ttttcctttt tctactcctt atcaatatca
     3241 gcagggcaat ttgtgtgtaa tggtctatca ttcacatact tatgctcttg cctgcactga
     3301 tcctttttta gcgcaaacgg ttccggaaat gagagcttta aaacgctgga ccgatgctta
     3361 tgatcccgat ccttataatc ctcctgttta taatgaatta aatggtaaaa tgccgaaaac
     3421 cgcattttat ttgattcctg agattcctgt gcaggatgaa tatcttcccc aagcaaagac
     3481 aataggttgc tatcccaatc cctttaaaga aactactacc ctgcagctga atctggataa
     3541 aaaacaggag gtctgcctgg aaatatacaa tatcaaagga cagaaagtgc aaactctgtt
     3601 taacggtttg gcagaaaaag gcgaaacgga aatttcttgg aatggtttag acaataacaa
     3661 cagaacagtt gccaacggct tatatttttg taagctgaaa acaaaggaca ggtgtgaaac
     3721 agctaaactg attgtattga aataaaacta aatataatta accgcgagtt caaggcatct
     3781 ttgattcaat agttgcttta gcaccttact ataggcaaat agagtaattg gtacttgaca
     3841 aataaatcaa agcttataaa ttagaaatat aagaaaagga gttgactatg gatatcaagg
     3901 aaatactctc acaaatccgc tccatagcaa ataatgcttt gggtggtggt tattctttcg
     3961 gacagccctc aaaattgggt gatctttatg taattccggt agccagagtt acttatggaa
     4021 tgggtggcgg agctgatgaa tcctctcccg atactgctaa aaaagaagag gaagacagct
     4081 catccccagc agaagaaaaa ggcaaaaagg taaaaaataa agttggtttc agcggaggtg
     4141 gaggtattgg cattcatagc gttccggtag ggcttttttc ccttaaggaa gagcgcatta
     4201 agtttcatcc agtaattggt ttttgggaaa ttattgcgat gatgagttta tcagctctgt
     4261 ttgtcctgaa aatgaaaaag ttgaggtata aaaagtaaac ccgaaatata ctgtttaggt
     4321 aaaaacggat tggtagtttc ttctttgctc agagggaatt tgaatacctg caaaactgct
     4381 ttaggtgatg aatatgcttt caatttttag tcccagtaac tatgcccctt ttaatattgc
     4441 ctgcgaggaa tatatcctga agaactttcc tgaggatgta tttttgcttt atataaacaa
     4501 ccccagtatt attgtaggta aacatcaaaa cactttggca gaaataaatt acgaatgggt
     4561 aagggaacat aatattccgg ttgtgcgtcg tttaacaggt gggggttctg tatttcacga
     4621 tccaggaaac ttgaattatt cctttttgat gaatgaaagt gaggatttta accgtaattt
     4681 tgaacgctat accaaaccca ttttagctgt ccttcaggag ttgggaattc ctgccatttt
     4741 agaaggaaga aacgacctta ctatcaatgg ctgcaagttt tccgggaacg ctaaaaccaa
     4801 tgccttcgga aaaactctcc agcatggaac tatccttttc agctccaata tttcagacct
     4861 caccgcggct ctaaaaccga gagaagaaaa gtttaacgat aaagcggtaa aatccgtgca
     4921 ggcaagagtt acaaatgttt ccgaccacct tccttatcct ctttctttgc aggattttgt
     4981 cactttagtt agggcaaaag tgagaactat gtatcccgat attcaggatt atactttttc
     5041 cctcagagat aaagaggaaa tccaagctct aatgaacagt aaatatgata cttgggaatg
     5101 gaactttggc aaatcacccc gctataattt gtgccattcc cttaaaacaa aagtaggaat
     5161 aattgaattc tacctcctgg taaataaagg tatcattgaa gaagtgaata tctacggcga
     5221 tttttttact aaccgcgaaa ttagcgaact ggaaaatgcc ctctgcggaa ttgaacataa
     5281 acctgagaaa gtagcggaag tgctgaaaga tatggaatat aaaagctttt tcggagaggt
     5341 gaaactggaa gaaatcgtga aggcaatgtt ttgattttta caaagaggaa aagagaaaaa
     5401 gagaaatgac aagaaagtag aaaaaccaga aagggggaca catttatgta gagggtggcg
     5461 taagcccctc gggaaaagaa gaatattact attccctctc tatttatttt tcaggtttc
//
LOCUS       DAOPFL010000099         5203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_21189_length_5203_cov_99.553613, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000099 DAOPFL010000000
VERSION     DAOPFL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21189_length_5203_cov_99.553613"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..1608)
                     /locus_tag="PLG69_06460"
     CDS             complement(388..1608)
                     /locus_tag="PLG69_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPI43188.1"
                     /translation="MKLEGKVVIAQGGGPTAVINQSLVGVTLEARKFPQVTRVYGALY
                     GVQGIVNEEFVDLTQETTHNLEQVANTPSSALLSTRDKPDEKYCMEIFKVLKAHDVRY
                     FFYIGGNDSADTVRIVNEQATQADYEFRAIHIPKTIDNDLVLNDHTPGFGSAARFVAS
                     AFTGVNLDNRALPGVYIGVVMGRNAGFLTASSVMAQKYPDDGPHLVYLPEHPFHRENF
                     LDDVKRIYDLYGRCVVAVSEGIVDENGVPIVTKLSGNQEKDAHGNVQLSGTGVLGDLL
                     SELVRTKLKIKRVRSDTFGYLQRSFIGCVSDVDQREAREVGERAAHYAIWYNLDGSIS
                     IQRTGFYSVNYQLENLSDIAGKTRTMPDEYINAEGNGITDEFKYYVRPLLGSGFPVAH
                     RLRAPKVPKLLEDK"
     gene            1752..3353
                     /gene="dnaX"
                     /locus_tag="PLG69_06465"
     CDS             1752..3353
                     /gene="dnaX"
                     /locus_tag="PLG69_06465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPI43189.1"
                     /translation="MSYIVLARKYRPQNFKEVYSQEHITEILQSAIATNRIAHAYLFT
                     GPRGVGKTSLARILAKSLNCVNGPTTTPCNACSNCLEITAGTSPDVIEIDGASNTSVD
                     DIRELQRELLYPASSSNYKIYIIDEVHMLSKNAFNALLKTLEEPPDNVIFIFATTEPQ
                     KVLPTIISRCQRYDFKRIPIASIVQRLQEISVLEGIKIDSESLYLIARKADGGMRDAL
                     SLMDQAISYCLNNITIDKVRQIFGMIPNQVYQKFLTLIYNKDTQGLINELHLIFEQGI
                     DLQEFIANMQEFVRILILRKLQVQIKDINPDELSLFDELAELFGQNKLLYILSYLIAC
                     RNDLRGSSNPYLILEALMIKLCKLDEMEDIASLIGKININAFAQPVSMASPTTAKTIQ
                     SSYVASSAEVPLREETIVAKMEFNQENLEQLWQRIITRAKKISQMCALALDATTDRKV
                     EGRKLCLTVESNTNFRCLKSNQEKLEESISELFEKPISISLQLQETEPPTKVNIQHKT
                     LEDIKQENPELAKFIDLTDSVLVKS"
     gene            3353..4456
                     /locus_tag="PLG69_06470"
     CDS             3353..4456
                     /locus_tag="PLG69_06470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI43190.1"
                     /translation="MKKCFPLFTILYSTFLIWLKRGVDKIIAEEKKKSVPDIFQSISI
                     IIAARNEIKNLPNLLNSLACLDYPRDKYEIIIVNDHSNDGSKEYLATQKNIPNLKIIN
                     FYSETPPLTGKKAALQKGIENSRFEILAFTDADCIVPVSWLQEINRSFTKETDYLLSY
                     SVIKRKPDDSIIRLKNFERSVNYALAAAGLYYKIPFTSSACNMVYRKKTFLESGGFEG
                     IGHLRSGDDDLLLIKMMPNIRKAAYNPNTAMQVTSIDGTDRKKHHQTNIRRASKFKYY
                     PWWLKGLCAFIFIYFCLFYIALWQAIHKKSGKAIKLSLLLKTAAEFAFSYSHLRQIGR
                     SKLAFLYPLQIFVFPLHFIFYGLRGTLGKYTWK"
     gene            complement(4457..4705)
                     /locus_tag="PLG69_06475"
     CDS             complement(4457..4705)
                     /locus_tag="PLG69_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43191.1"
                     /translation="MVYKIVLVKIDQRSTEATKVQGILTKYGCNIRVRLGLHEVSNEF
                     CANDGLVVLEVQGEQNILENMISELNALEYVQVRLIEM"
     gene            complement(4718..>5203)
                     /locus_tag="PLG69_06480"
     CDS             complement(4718..>5203)
                     /locus_tag="PLG69_06480"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424926.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HPI43192.1"
                     /translation="KGWFRLALGKEVRLKYAYYITCNEVIKNEKGEIIELLCTYDEKS
                     RGGWTEDGRKVKGTLHWVCAEDAIPVELRLYDRLFTLADMSNMEEGKTYQDYLNPNSL
                     IVKQNCLAEENLQEAKIGEHYQFLRIGYFNVDYNSKPGALIFNRIVDLKDSWAKLSKK
                     I"
BASE COUNT         1664 a         1048 c          882 g         1609 t
ORIGIN      
        1 catcccagat tgtttctact acttctttaa ctgcctgtaa gaattcaggt tcacctggat
       61 tcttggcttc cacttttgcc ataaactcat tgatggtcac gagttcctcc atatattata
      121 ttgtttttct taacctaaaa ataattctat caatttatgt caagtaggaa aaagaaaagg
      181 ttaacgggat aacgagttaa cgggtgaaca ggtgaacagg ttaacaggga aataaaaatc
      241 gttgcaaact ctgaaatggg caattggagc ttgctgatgc tctgcattta tgaacaatgg
      301 cgtcttcttc ggttcatcta taatcgttac aagtcctcgc tgcaaaataa aattccttta
      361 ttctttaaaa tcctgcttat tccagtccta tttatcttcc agcaacttag gaactttggg
      421 tgcacgcaaa cgatgagcta caggaaaacc tgaacccaaa agaggtctta cataatactt
      481 gaattcatcg gtaattccat ttccttcagc attaatatat tcatcgggca tagttcttgt
      541 ttttccggca atatcgctta aattttccag ctggtaattt acggaataaa atcccgttct
      601 ttgaatagaa attgagccat ccaaattata ccagatagca taatgagctg ctctttctcc
      661 aacttcgcgt gcttcccttt gatcaacatc acttacacag ccgataaaag aacgctgtaa
      721 ataaccaaaa gtatcactac gcactctttt aattttcagt ttagtgcgaa caagttcact
      781 aagtaaatca cccaaaactc cggttccgga aagctgaaca ttgccatgag catctttttc
      841 ctgatttcct gaaagttttg taacaatggg cactccattt tcatcaacaa taccttcgga
      901 aacggctact acacatctac cgtaaagatc ataaatgcgt tttacatcat ctaagaaatt
      961 ttcccggtga aaaggatgtt caggtaaata tacaagatga ggtccgtcat caggatattt
     1021 ttgtgccata actgaagaag cagttaaaaa accggcattc cgacccataa ccactccaat
     1081 ataaaccccg ggtaaagcgc gattatctaa attgactcct gtaaaagcag aagctacaaa
     1141 tcgtgctgca gagccaaaac caggtgtatg atcattcaaa accaaatcgt tatcaatggt
     1201 tttaggaata tgaatagccc gaaattcata atccgcttgg gttgcctgtt catttacaat
     1261 gcgaactgta tcggcagaat cgttgcctcc gatatagaaa aaatatctga catcgtgtgc
     1321 ttttagcact ttaaaaattt ccatacaata cttttcgtcc ggtttatccc gcgtagaaag
     1381 taatgctgaa gatggtgtat tagccacctg ttccaaattg tgagttgtct cctgggttaa
     1441 atctacaaat tcctcattca caattccctg aactccatat agagctccat aaacacgcgt
     1501 aacttgggga aattttcttg cttccagggt tactcctact aaggactgat tgattacagc
     1561 agtaggacct ccgccttgtg ctataacaac ttttccttcc agtttcattt taaatcctcc
     1621 attctgatta aacttctgaa aacacttaaa acaaagtgct gataatctgt ccagcagaaa
     1681 atacttgctt aaaacagtgt ctgtagaaag atggtaaaat aaatgtattg tattatattt
     1741 cgtgaggata aatgagttac attgtcttag ccagaaagta ccgcccccag aactttaaag
     1801 aagtttactc tcaggagcat ataacggaaa tcctgcaaag tgccatagct acaaatagaa
     1861 tagctcacgc ttatttattt acaggacccc gaggtgtagg aaaaacctca ttggcaagaa
     1921 ttttagcaaa gagtttgaat tgtgtaaacg gaccaactac tacaccttgt aatgcctgtt
     1981 ccaattgttt ggaaattacg gcaggaactt ctcctgatgt aattgaaatt gatggtgctt
     2041 ccaataccag tgtagatgat attcgtgaat tgcaacgcga gcttttatat cctgccagca
     2101 gttccaatta taaaatatac atcattgacg aagtgcatat gctctctaaa aatgctttca
     2161 atgcattact taagaccctg gaagaaccgc cggataatgt tatcttcatt tttgccacaa
     2221 cagaacccca aaaggttttg ccaactatta tttctcgttg tcagcgttat gattttaaac
     2281 gtattccaat tgcttccata gttcagaggt tgcaggaaat aagcgttctg gaagggataa
     2341 aaattgattc cgaatccctt tatttaatag ctcgtaaggc agatggagga atgcgcgatg
     2401 ccctatcgtt gatggatcag gcaatttcat attgtttgaa taatattact attgacaaag
     2461 tccgtcaaat atttggtatg attcccaatc aggtctatca gaaatttcta actctgattt
     2521 acaataaaga tacccagggt cttattaacg aattgcattt gatttttgaa cagggaattg
     2581 acctccaaga atttatagcc aatatgcaag aatttgtgag gatccttata ttgcgtaaac
     2641 tgcaagttca aattaaggat atcaatcccg atgaactttc tttgtttgat gaattagctg
     2701 aattattcgg gcagaacaaa ttgctgtata ttctttccta ccttattgcc tgtagaaatg
     2761 atttaagggg aagtagcaat ccctacctta ttttagaagc tctgatgatt aaactttgca
     2821 agcttgatga aatggaagat attgcctctt taattggcaa gattaatata aatgcctttg
     2881 cccaacctgt atctatggct tctccaacaa ccgctaagac aattcaaagc agttatgtag
     2941 ccagttcggc agaagttcct ttacgcgaag aaacaattgt ggctaaaatg gaatttaacc
     3001 aggaaaatct ggaacagctt tggcagagaa ttattacccg cgctaaaaaa atcagtcaaa
     3061 tgtgtgctct tgctttagat gcaactacag acagaaaagt ggaagggcga aaactttgtt
     3121 taacagtaga aagcaatact aatttccgct gtttgaaaag caatcaggaa aagctggaag
     3181 aatctatttc cgaactcttt gagaaaccga tttctatctc tttgcaattg caggaaacgg
     3241 aaccccctac caaagttaat attcaacaca aaacactgga agatataaaa caggaaaatc
     3301 cggagctggc aaaatttata gaccttacag attccgtgct ggttaaaagc taatgaagaa
     3361 atgctttcct ctatttacaa tattgtattc tacttttcta atttggctaa aacgaggcgt
     3421 agataagata attgctgaag aaaaaaagaa atcagttccc gatatttttc aaagtatttc
     3481 catcataatt gcagccagaa atgaaataaa gaatctgcca aatttactaa actcccttgc
     3541 ctgtttggat tatccccggg acaaatatga aataattatt gtaaatgacc atagcaacga
     3601 tggcagtaaa gaatatttgg ctactcagaa aaatattcct aatctgaaaa ttattaattt
     3661 ctatagtgag accccaccct taacaggaaa aaaagctgct ttacaaaaag gcatagaaaa
     3721 ctcccgcttt gaaattcttg ctttcactga tgctgattgt attgtgcccg tttcctggtt
     3781 acaagaaata aaccggtctt ttactaaaga aacagattac ctgctttcct attcagttat
     3841 aaaaagaaaa cccgatgaca gcatcattcg tctgaaaaat tttgaacgca gtgttaatta
     3901 tgctttagct gcagcgggct tatattacaa aattcctttt accagcagtg cctgtaatat
     3961 ggtttacagg aaaaagacct ttctggaaag tggtggtttt gaaggaatag gacatttaag
     4021 atcaggtgat gacgatttac tattaataaa aatgatgcct aatatcagaa aagcagcgta
     4081 caatcccaat acagcaatgc aggttacctc tattgatggc acagacagaa aaaagcatca
     4141 tcaaacaaat atccgcagag cttccaaatt caaatattat ccctggtggc tgaaaggtct
     4201 ttgtgccttt atttttatct atttttgtct gttttatatt gctctgtggc aagctataca
     4261 taagaaaagc ggtaaagcaa ttaaactttc tttactgctt aaaacagcag ccgaatttgc
     4321 tttttcctat agccatttaa ggcaaattgg aagaagcaaa ctcgcttttt tgtatcctct
     4381 tcagatattt gtatttcccc ttcacttcat tttctatggc ttgcggggaa ctttaggaaa
     4441 atatacctgg aaatagttac atttcaatta acctaacttg cacatattcc aaagcattaa
     4501 gttctgaaat catattctcc agaatatttt gttctccttg cacctccaga acaactaaac
     4561 catcattggc gcagaattca ttagagacct catgcaaacc taaacggacg cgaatattgc
     4621 atccatactt ggttaaaatt ccctgaactt ttgtagcttc agtgctacgt tgatcgattt
     4681 ttacaagcac aatcttgtaa accataatat cctcccctta tattttctta ctgagctttg
     4741 cccagctgtc ttttaagtcc acaatacggt taaaaatcaa tgcccctggc ttactgttat
     4801 aatcaacatt gaaataacca attcgcagaa actgataatg ttctccaatc tttgcttctt
     4861 gcaaattttc ttctgctaaa cagttttgtt taactatcag cgaatttgga ttcaagtaat
     4921 cctgataggt ctttccttct tccatattac tcatatccgc taaagtgaaa aggcgatcat
     4981 ataaacgcaa ttctacagga atagcatctt ccgcacaaac ccaatgtaaa gttcctttca
     5041 ctttgcgtcc atcttctgtc catccgccgc gtgatttttc atcgtaagtg caaagcaatt
     5101 ctataatttc gcccttctca tttttgatta cttcattaca agtgatataa taagcgtatt
     5161 ttaagcgaac ttctttgccc aaggcaagac gaaaccaacc ttt
//
LOCUS       DAOPFL010000100         4964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_22657_length_4964_cov_114.630475, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000100 DAOPFL010000000
VERSION     DAOPFL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22657_length_4964_cov_114.630475"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLG69_06485"
     CDS             <1..342
                     /locus_tag="PLG69_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPI43193.1"
                     /translation="LGRYSGEDLAQAGWGILMTADKQYRLAHCALSPIPKRAYYIEDL
                     LNNSNPLNADLIEKAIALIPSEINPITDIRATQEFRMYISGIMLKRGLTAIQERISGK
                     YIEPRKLLGDV"
     gene            342..806
                     /locus_tag="PLG69_06490"
     CDS             342..806
                     /locus_tag="PLG69_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPI43194.1"
                     /translation="MKEISLILNNEPRNVQVENNEILLDVLRNKLGIKSPKCGCERGD
                     CGACTVLLDGKAVRSCLILAVEAENHNIITLEGISREKLSALQEAFIEFNSFQCGYCA
                     PGIILAVTELLEKNPHPSLEEIKESIAGNLCRCTGYTPIFDAIMSLTGRGAK"
     gene            803..3142
                     /locus_tag="PLG69_06495"
     CDS             803..3142
                     /locus_tag="PLG69_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPI43195.1"
                     /translation="MKDYKYIGKDVTRIDALDKISGAATFVDDIDFGANLLYAELIES
                     TQASALIKNIDVSEALKVKGVYKIFTGKDFHNRFGLYMKDRYVFAQERVRFVGEQIGA
                     VVAFTPEAAKSAVSLVKVEYEPLPAVLNQMDALKSDAPLLHPEVENYPRVPWFYPKGG
                     TNIAHWRKTRKGDLEKGFANADVILEETYSVPRYAHCALETHIAVAKFDHSGRLTVWS
                     SSQSPHTQRNLFAEALGLSHKDVRVITPYVGGGFGGKAGVTMEIIPAVLATQLKGYAV
                     KLRYTREQEFTNTYQRLGLIAKIKMGATKEGIITALEHQLYWDAGAYVEYGANVVNAV
                     GLSASGPYRIDNVSIDSICVYTNLPPGGPYRGFGYSEMLFGLESHISRMAHQLNMDEV
                     EFRRINAIKPGDKTAYGAKMNPSGLQECIDKAAKAIDWGKKPISEDPTKVIGQGFAIF
                     WKAPAMPPNASSSAFLKFNEDATINLLISGMDIGQGYQTVMAQIAGEILSVPPAKIRV
                     ETPDTDRNPYEWQTVASHITWSCGNAVKNAALDAREQIFTLIHRVYHFPLDALYLEDE
                     AVKCKTDPAFILPLKNFVVEGIMTEDGTFKGGPINGRGMFMPEFASTKDNPETSQGGH
                     PNVHYTVGAAGLNLEIDQETGKMKVLKVALAIDAGKAINPDLVKGQIMGGLLQGLATV
                     LYEDMRFNEKGKLLNPNLTDYKIPTAKDVPEEIIPIIVEVPQPDGPFGARGIGEHTML
                     PAAPMIANAIEDALGIRIKSLPITAEKVALTCLREKAKQ"
     gene            3139..4329
                     /locus_tag="PLG69_06500"
     CDS             3139..4329
                     /locus_tag="PLG69_06500"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HPI43196.1"
                     /translation="MNKPLQDVVVLDLSRVLAGPFCTMILSDLGAEIIKVEHPEKGDD
                     SRAYGPFLHNRSLYFLSINHGKKSISLNLKKPQGRELLLKLIEHCDVLVENFRPGMME
                     KFNLGYELLKEKYPQLIYAAVSGFGHSGPDSQKPAYDILVQARGGIMSITGWQNMAPT
                     RVGMSLGDITASLFTAIGINSALYQRTKTGLGQKIDVAMLDGQIAILENALVRYQAEG
                     ISPKPLGNRHPTISPFQAYKASDNYFVIAVGNDNLWDTFCKALEIPELISDKRFCNNR
                     LRTENIDALNAILEPIFTAKPASWWLELMEKSGIPCSSINNIESVMQDPQLLARNMFV
                     EVNDPIAGTVKIAGNPIKMSSFPDSAVYEPAPEIGAHNKEIYCERLGLSEEDLAELQK
                     AGVI"
     gene            4375..>4964
                     /locus_tag="PLG69_06505"
     CDS             4375..>4964
                     /locus_tag="PLG69_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPI43197.1"
                     /translation="MNSAIFPDNLKELSFALRQGKLPLQDYLFQTLADIEKKEPVLCA
                     LEPEENRQERLLKQAEELLSRFPQPELRPPLFGVLIGVKDLFNVDGLPTRAGSKLPPE
                     VFSGKEAEIVTRLKNLGALILGKTTCTEFAYFQPAKTKNPCNPNHTPGGSSSGSAAAV
                     SAGYCLLALGTQTIGSIIRPASYCGVTGLKPSYGLFS"
BASE COUNT         1465 a          908 c         1124 g         1467 t
ORIGIN      
        1 ctgggtcgtt acagtggaga agaccttgct caggcaggtt ggggaatttt aatgactgcg
       61 gataaacaat atcgtttggc tcattgtgct ttaagcccta ttcccaaaag agcttactat
      121 attgaagacc tcttaaataa cagtaaccct ctgaatgctg atttaataga aaaagccata
      181 gctcttattc cttcagaaat aaaccccata acagatattc gcgccacaca agaattccgg
      241 atgtatatta gtggaattat gcttaaaaga ggtttaacgg caattcagga aagaatttcc
      301 ggcaaatata ttgagccccg aaagcttttg ggagatgtat gatgaaagaa atcagcttga
      361 tacttaataa tgaaccccgt aatgtgcaag tggaaaataa tgaaatactt ctggatgtgc
      421 tccggaataa attgggaatt aaaagtccca aatgtggctg tgaaagaggt gattgcggtg
      481 cctgcactgt tttgcttgat ggtaaagcag ttcgcagttg tctgattctt gccgttgaag
      541 cggaaaatca taatattatc actttagaag gtatttccag ggaaaaactt agtgctctgc
      601 aggaagcatt tattgaattc aattcttttc aatgcggtta ttgtgcaccg ggaataattt
      661 tggcggttac tgaattgctg gaaaaaaatc cccatccctc tttagaggaa attaaagagt
      721 caattgccgg aaacctttgt cgttgtacag gttatacacc tatttttgat gctattatgt
      781 ctttaaccgg aaggggggca aaatgaagga ctataagtat ataggcaaag atgtaacgcg
      841 tattgatgct ttggataaaa tcagcggagc agctactttt gttgatgata ttgattttgg
      901 agctaatctg ctttatgcgg aattgattga aagcacgcaa gcttctgctt tgatcaagaa
      961 tatagatgtt tctgaagcat tgaaggtgaa aggcgtttat aaaatattta cagggaagga
     1021 ttttcacaat cgtttcggac tttatatgaa agaccgttat gttttcgctc aggaaagagt
     1081 ccgttttgta ggtgaacaaa taggtgcggt agtggctttc actccggaag ctgcaaaaag
     1141 tgctgtttct ttggttaaag tggaatatga accccttccg gcagttttaa atcaaatgga
     1201 tgctctgaaa agtgatgctc ctttactgca tccggaggta gaaaattatc ctcgcgtacc
     1261 gtggttttat cccaaaggcg gaacaaatat agctcattgg cgaaaaaccc gcaaaggtga
     1321 tttggaaaaa ggttttgcca atgcagatgt tattctggag gagacatatt ccgttcctcg
     1381 ttatgcacat tgtgctttgg aaacccatat agcggttgcc aaatttgacc attcgggacg
     1441 attaaccgtt tggagcagtt cacaatctcc gcatacccaa agaaatcttt ttgccgaagc
     1501 attaggcctc agccataaag atgtaagagt tataactcct tatgtgggag gtggttttgg
     1561 aggaaaagcc ggagttacga tggaaattat tcctgcagtt ttagccactc aactaaaggg
     1621 ttatgcagtt aagttgcgtt acaccaggga acaggaattt accaatactt atcagcgatt
     1681 gggattgatc gcaaaaataa aaatgggtgc tacaaaagag ggaattataa cggctttgga
     1741 acatcaatta tattgggatg caggtgctta tgtagaatat ggagcaaatg ttgttaatgc
     1801 agttggttta tcagcaagcg gaccttatcg gattgataat gtttccatag attctatttg
     1861 tgtgtatacc aatcttcctc cggggggtcc ttatcgcggt tttggctatt cggaaatgct
     1921 ttttggcttg gaaagtcata tcagtagaat ggctcatcaa cttaatatgg acgaagtaga
     1981 atttagaagg ataaatgcca ttaagccagg agataaaacc gcttatggtg ctaaaatgaa
     2041 cccgagcggt ttacaggaat gtattgataa agccgcaaaa gcaattgatt ggggaaaaaa
     2101 acccatttcc gaagacccga caaaagttat tggtcagggc tttgctattt tttggaaagc
     2161 gcctgctatg cctccgaatg cttcttcttc cgcattttta aagttcaatg aagatgccac
     2221 aatcaatctc cttatttccg gtatggatat cggacaaggt tatcagacag ttatggctca
     2281 aattgcagga gaaattttat ctgttccacc agccaaaatt agagtagaaa cacctgatac
     2341 ggatcggaat ccttatgagt ggcaaactgt tgcaagtcat ataacctgga gctgtggaaa
     2401 tgccgttaaa aatgccgcat tggatgcccg ggagcagatt tttacgctca ttcacagggt
     2461 ttatcatttt ccgttggatg ctctttatct ggaagatgaa gcagttaaat gcaaaaccga
     2521 tcccgctttc attttacctt tgaaaaattt tgtggtagag ggcattatga cggaagacgg
     2581 aacttttaag ggtggtccta taaacggtcg cggaatgttt atgcctgaat ttgccagcac
     2641 taaagacaat ccggaaacaa gtcagggagg tcatcctaat gttcattaca cggtaggtgc
     2701 ggcaggtttg aatttggaaa ttgatcagga aacgggcaaa atgaaagtgc tgaaggttgc
     2761 tttagccatt gatgccggaa aagccatcaa tcctgatttg gtaaaaggcc aaataatggg
     2821 aggtctgctt cagggtttag ctactgtgtt atatgaagat atgcgtttta acgaaaaagg
     2881 taaactgctt aatcctaatc ttacggacta taaaattcct actgctaaag atgttccgga
     2941 agagattatt ccgattattg tagaggttcc tcaaccggat ggtccttttg gagcgcgggg
     3001 aattggagaa catacaatgc ttcctgcagc tccaatgatt gccaatgcca ttgaagatgc
     3061 tctcggtatt agaattaagt ccctacctat aacggcagag aaagtggcat taacttgttt
     3121 gcgggaaaag gcaaaacaat gaataaaccg cttcaagatg ttgtagttct tgaccttagt
     3181 cgtgttctgg caggtccttt ttgcacaatg attttaagcg atttgggcgc agaaataatc
     3241 aaggtggaac atcctgaaaa gggcgatgac tccagagctt atggtccttt tctgcacaat
     3301 cgttctctct attttctcag tatcaaccac gggaagaaaa gtatctcgtt aaacctgaaa
     3361 aaaccgcagg ggagagaatt gcttttaaag ctgatagaac attgtgatgt tttggtagaa
     3421 aatttccgcc ctggcatgat ggagaaattt aacctgggat atgagttatt aaaggaaaaa
     3481 tatccacaat tgatttatgc tgccgtttcc ggtttcgggc atagcggacc tgattcccaa
     3541 aaacctgcct acgatatttt agttcaagcc aggggcggta ttatgagtat tactggttgg
     3601 caaaatatgg ctcctacacg cgtaggaatg tctcttggag atataactgc ctcgcttttc
     3661 actgccatcg ggattaattc tgctttatac caacgcacaa aaacaggttt gggacaaaaa
     3721 attgatgttg ctatgttgga tggtcaaata gccattctgg aaaatgcttt agtgcgttat
     3781 caggcagaag ggatttcacc caaacctctt ggcaatcgtc atcctacaat atctccattc
     3841 caggcatata aggcaagcga taattatttt gtgattgccg tggggaatga taacctttgg
     3901 gatacctttt gcaaagctct ggaaattccg gaattaattt cggataagcg tttttgtaac
     3961 aatcgcttac gcacggaaaa tatagatgcc ttaaatgcca ttttagaacc tatttttacg
     4021 gcgaaacctg cttcctggtg gctggaacta atggaaaaat ccggaattcc ctgttcttct
     4081 atcaacaaca ttgaatccgt aatgcaagac cctcaattgc ttgctcgtaa tatgtttgtg
     4141 gaagttaatg accctatagc gggcactgta aaaattgccg gaaatcctat aaaaatgagt
     4201 tcttttccag attcagcagt gtatgagccc gctccggaaa taggagcaca caataaagag
     4261 atttattgtg aacgacttgg cttaagtgaa gaggacttag ccgaattgca aaaagcagga
     4321 gtgatttaac atcaatattc ccttatgttt ccttaaaatc cggaaggata ttagatgaat
     4381 tccgctattt ttcccgataa cctgaaagaa ctttcttttg ctttacggca gggaaaactt
     4441 cctttgcagg attatctttt tcagaccctt gccgatattg aaaaaaagga acccgtttta
     4501 tgtgctctgg aacctgaaga aaacaggcaa gaacgcttac tgaaacaagc tgaagaactg
     4561 ttatcccgtt ttcctcaacc ggaattgcgt ccacctcttt ttggggtttt aattggcgtt
     4621 aaagatttgt ttaatgtgga tggtttacct acgcgtgcgg gttcaaaact tcctccggag
     4681 gttttttccg gcaaagaagc ggaaattgtt acccgcttaa aaaatttggg agcactaatt
     4741 ttagggaaaa ccacctgcac tgaatttgct tattttcagc cggcaaagac aaaaaatccc
     4801 tgtaatccga atcatactcc gggtggttct tccagtggat cagcggcagc tgtatctgca
     4861 ggatattgtc ttttagcttt gggaactcag accattggct ctattattag gcctgcttct
     4921 tactgtggag ttacaggttt aaaacctagt tatggtttgt tttc
//
LOCUS       DAOPFL010000101         4848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_23417_length_4848_cov_100.363864, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000101 DAOPFL010000000
VERSION     DAOPFL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23417_length_4848_cov_100.363864"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..944)
                     /locus_tag="PLG69_06510"
     CDS             complement(12..944)
                     /locus_tag="PLG69_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43198.1"
                     /translation="MKRFIFFLLCLLMCISLFAIDNKFLHQLVQVKSSQAYSIRFDDD
                     LVYTNNQNYIWVYSIFNAWQPKLEAAFFSPNPIEDIETLAGKHLYVASNEPTNQVILI
                     DSLYTGSRIFFPQTIVGDKLTREGSILYVADRYRGIDIINLSGGSTRELLATFSEKWG
                     IKDFVASYPYIFALNDFGLVAVDITDQSYPVSLGVNYEIIDATCLVKNGNTIWIGAGK
                     NLLAFNVYEPKKPTLISQIRMTNEILSLDIKDNRLFIALGRGGVKIIDVTNPLKTEDI
                     NNIFLTIPVYDLDVANDYIFLGLGKEGWMLYEYR"
     gene            complement(941..1927)
                     /locus_tag="PLG69_06515"
     CDS             complement(941..1927)
                     /locus_tag="PLG69_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPI43199.1"
                     /translation="MTKEELIQDITAMQSLKETLLNEIHNVIIGQDRVLTDVLIALFA
                     NGHILLEGVPGLAKTLLISSLAKALSLSFSRIQFTPDLMPSDITGTEILVNDPITEKK
                     HFEYIKGPIFANIILADEINRTPPKTQAALLQAMQEYAVTSGTVTRPLSKPFLVMATQ
                     NPIEQEGTYPLPEAQLDRFMFFINIDYPTLEEELQIAESTTGLENPVITPQLTGQQII
                     SLQQAVRSLPVSDHVLKFAVNLVRKSRPNTDDALNEIKQWVAWGAGPRASQYLILSAK
                     ARAALDGRLTPAEEDVKASAINVLQHRILPSFAAQAEGINSKQIINWLLEQK"
     gene            complement(1920..2924)
                     /locus_tag="PLG69_06520"
     CDS             complement(1920..2924)
                     /locus_tag="PLG69_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPI43200.1"
                     /translation="MTRKNRNSLVIGLIIGILLLAAWLYYTPLEEIKAQIAQVNYNWV
                     IIASVAYLSAYFLRSLRWRLLIPAPANPGIFKTWLYAMAGNLLNYLIPIRAGDFARAW
                     FIKKNYSLPYMKALPSVFVDKVFDTIAILFIIIVLPFTTIELSTPMVILLCLLLLIFI
                     VAFAILLISVWKKELAVKIISSLFSWLPQKAKAKINPAIEMFISELNLFEHHTFKLVS
                     ALFLTAGGVLLDGIYFYLLFRAFGILYPFALVLFGYTLINLSYAIPQPPAQLGSNEWM
                     MIIIFSIGFGLTKTTASAIMAFGHILTAGLMSLWGIIAFAVLGPELLFTAIKGDKIND
                     "
     gene            complement(2921..3760)
                     /locus_tag="PLG69_06525"
     CDS             complement(2921..3760)
                     /locus_tag="PLG69_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPI43201.1"
                     /translation="MAVPIINDWAKYFDNPDEGLGSSYERIVLNKLLDFVCKTYKIQS
                     ALETPCFGFTGVSGINLLNLARQGIKVSLEDHNLERIEKIRELWQELRLHTDIKYNLD
                     YVKLDYPDKHFDLGFNFSALWFTQNIRSFLSEFCRVCKKAILICVPNRNGIGYKMQIK
                     DYSPEKYPFLHPEYLDPATIKFLMKQNKWQLQDENYIDCPPWPDIGMKKELFFNRLLK
                     KQEIQENNTEPVTILTYYQDEDSEFAEKMFKYSFVERTAPKQFKKYWAHHYYLLFTPE
                     NSE"
     gene            complement(3760..4743)
                     /locus_tag="PLG69_06530"
     CDS             complement(3760..4743)
                     /locus_tag="PLG69_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPI43202.1"
                     /translation="MAKILITGITGFIGRNVLREMLKSCPDFNITALVRPKTEPRRYQ
                     EFEKAIRLVAIDLADTASLKEFLFTNEFDTILHIGALRGGRKFPQETYLRSNVYSTGQ
                     MVEYCLAKDARLIYCSSVGVFGAIPQELPAGPQTEKNPDNYYHYTKIESERIINRAIL
                     NGLNAGIVRPSIAYGEGDFGFPYQMVKLVDKHLFPLINRPIWIHLCHIDALVKAFLYL
                     ATHEWKSGLTLTVADREPVRLQSLVDFISRQLHGKNYPRALRFDRIFFAWGEKLARLV
                     KNELWISRFELISKSWFYDVSAYYDIMEKEGYKSHFTIPEFEITIKDYQKH"
BASE COUNT         1560 a          911 c          953 g         1424 t
ORIGIN      
        1 gccctctgct tttaccggta ttcatagagc atccaaccct cttttcccag tcctaaaaaa
       61 atgtaatcat tagctacatc caaatcgtaa acgggaatag tgagaaagat gttgttgata
      121 tcttctgtct tgaggggatt agttacatca ataattttaa ctcccccgcg acctaaggca
      181 ataaaaaggc gattatcttt aatatccaaa ctcaggattt cgttagtcat tctaatttgg
      241 ctgatgagag ttggcttttt gggttcataa acattgaaag ccaagaggtt ttttccggca
      301 ccgatccaaa ttgtatttcc gtttttaacc aaacaagtgg catctataat ttcgtaattt
      361 acacccaaag aaacaggata ggattgatcg gtaatatcta cggcaactaa accaaaatca
      421 ttgagagcaa aaatataagg gtaacttgcc acaaagtcct taatgcccca tttttcagaa
      481 aaagtagcta ataattcgcg tgtgcttcca ccgcttaaat taataatatc aattcctcta
      541 taacggtctg ccacataaag gatagaacct tcgcgggtaa gtttatcacc tacaattgtt
      601 tgagggaaga aaatgcggga gccagtatat aagctatcta tcagaataac ctgattggta
      661 ggttcattgg aagctacata aagatgtttt ccggcaagag tttcaatatc ctcaatcgga
      721 ttgggagaaa aaaaggcagc ttccagtttg ggctgccagg cattaaatat ggaataaacc
      781 caaatgtaat tctggttatt agtataaacc aaatcatcgt caaagcggat ggaatatgcc
      841 tgcgaacttt taacctgaac caattgatgc agaaatttat tatcaatggc aaagagggat
      901 atacacatta gcaggcaaag aagaaagaat ataaagcgtt tcatttttgc tccagtaacc
      961 agttaatgat ttgtttggag ttaattcctt ccgcttgagc tgcgaaggag ggtaaaatac
     1021 gatgctgtaa cacattgatg gcagatgcct taacatcttc ttctgcagga gttaaacgtc
     1081 catctaaagc ggctcttgct ttggcagaaa gaataagata ttgtgaagca cgcggtcctg
     1141 ctccccaggc aacccactgt ttaatttcat ttaaggcatc atccgtattg ggacgggatt
     1201 ttcttactaa atttacggca aatttaagaa catgatcact tacaggaaga gaacgcactg
     1261 cttgttgtaa agatattatt tgttgacccg tcagctgagg tgtaattaca ggattttcca
     1321 gtcccgtagt gctttcagca atttggagtt cttcttctaa agtagggtaa tcaatattga
     1381 taaagaacat aaaacggtct aactgtgctt ccggaagggg ataagttcct tcctgttcaa
     1441 tgggattttg agttgccatc actaaaaacg gtttacttaa gggacgggta acggttccgc
     1501 tggttactgc atattcttgc attgcctgta ataaagctgc ttgagtttta ggaggtgtgc
     1561 ggttaatttc atctgccaga atgatattgg caaaaatagg acctttgatg tattcaaagt
     1621 gctttttttc ggtaatgggg tcatttacca aaatctccgt tccggttata tcggaaggca
     1681 ttaaatcggg ggtaaattga atccgactga aagaaagcga aagtgctttt gccagagaag
     1741 aaataagcaa ggtttttgct aatcccggaa caccttccag aagaatatgt ccattggcaa
     1801 aaagggcaat gaggacatcg gttagaactc tgtcctgacc aattataaca ttatgtattt
     1861 cgttcagcag ggtttctttc aagctctgca ttgcagttat atcttgtatc agttcttctt
     1921 tagtcattta ttttatctcc tttaatagct gtgaataaga gttcaggacc aagaaccgcg
     1981 aaagcaatta ttccccataa actcattaat ccggcagtta aaatatgtcc aaaagccata
     2041 attgccgagg cagtggtctt tgtcaatcca aatcctatac tgaaaataat aatcatcatc
     2101 cattcgttac tgccaagttg agcgggaggt tgaggaatgg cataggaaag atttattaaa
     2161 gtatagccaa ataaaaccag agcaaaagga tataatattc caaaagctcg gaacagcaaa
     2221 taaaaatata ttccatccaa taaaacccct ccggcagtta aaaataatgc cgaaaccagc
     2281 ttgaaagtat gatgttcaaa gaggttaagc tcactgataa acatttctat agccgggttt
     2341 atttttgcct ttgctttttg gggcaaccag gaaaaaagcg aggaaataat tttcaccgcc
     2401 aattcctttt tccaaacaga aataagcagt atagcaaaag ctacaataaa gatcagcaat
     2461 aataaacaaa gtaaaatgac cattggagtg ctcagttcaa tggttgtaaa aggtaaaaca
     2521 atgatgataa acaagatggc aatagtatca aagaccttat ctacaaatac cgaaggcaat
     2581 gctttcatat agggtagaga ataatttttt ttaatgaacc aggcacgggc aaaatctcct
     2641 gctctgatag gaattaaata gttgagcaga tttcccgcca tagcatacag ccaggttttg
     2701 aatatacctg gattagcagg agcgggaata agtaaacgcc aacgcaaaga acgcaaaaaa
     2761 taggcagaaa ggtatgccac ggaagcaatt ataacccaat tataattgac ttgtgcaatt
     2821 tgggctttaa tttcctctaa aggagtataa tataaccagg cagcaagcaa aaggatgcct
     2881 ataattagac cgattactaa tgagttacga ttttttcggg tcattcacta ttttccggag
     2941 tgaagagcag ataataatgg tgagcccaat attttttgaa ctgtttggga gcagttcttt
     3001 ccacaaaact atatttaaac atcttttcgg caaattcact gtcttcatcc tgatagtaag
     3061 taagaatggt aacaggttcc gtattgtttt cctgaatttc ctgttttttc agcaggcggt
     3121 taaaaaagag ttcttttttc attccaatat cgggccaggg gggacaatca atatagtttt
     3181 cgtcttgcaa ttgccattta ttttgtttca tcaggaattt gatggtagcg ggatcaagat
     3241 attccggatg caaaaaaggg tatttctccg gtgaataatc ctttatctgc attttatagc
     3301 cgattccgtt tcggttggga acacaaataa gaattgcctt tttgcaaact ctgcagaatt
     3361 cggaaagaaa acttctaata ttttgagtaa accacaaagc ggaaaaattg aagcccaaat
     3421 caaaatgttt gtcaggataa tccagtttca cataatcaag gttatatttg atatcggtat
     3481 gtaaacgcaa ttcttgccat aattcccgaa ttttttctat gcgctctaaa ttatgatctt
     3541 ccagagagac ctttattccc tgtcttgcca aatttagcag atttataccg cttactccgg
     3601 taaaaccaaa acagggtgtt tccagagccg attgaatttt ataggtctta caaacaaaat
     3661 ccagcaactt attgaggaca atgcgttcat aagaagagcc caaaccctca tccggattat
     3721 caaaatattt tgcccagtca ttaataatcg gaacagccat cagtgtttct gatagtcctt
     3781 tatggttatt tcaaattcgg gaatggtgaa atgggatttg taaccttctt tttccattat
     3841 gtcgtaatag gcgctcacat cgtaaaacca gcttttggaa attagctcaa aacggctgat
     3901 ccataattca ttcttcacga gacgagcaag tttttcaccc caggcaaaga aaattctatc
     3961 aaatcttaaa gcacggggat agttttttcc gtgaagctga cgggaaataa aatctaccaa
     4021 tgattgtaaa cgcaccggtt cacgatctgc taccgtaagc gtaagtccac ttttccattc
     4081 gtgggttgca agatagagaa atgccttcac caaagcatca atatgacaaa gatgaatcca
     4141 aataggtctg tttataagcg ggaaaagatg tttgtctact aattttacca tttgataagg
     4201 gaaaccgaaa tcgccttctc cataagcaat actgggacgg acaattcctg cattcagtcc
     4261 atttaaaata gcgcgattga tgatgcgttc cgattctatt ttcgtgtaat gataataatt
     4321 atcagggttt ttctcggttt gcggaccagc aggaagttcc tgaggaatag caccaaaaac
     4381 acctacggaa gaacaataaa tcaaacgggc atctttggca aggcaatatt ctaccatctg
     4441 tccagtgctg taaacattac tgcggagata tgtttcttgg ggaaattttc tgccaccgcg
     4501 taatgcacca atatgcaaaa tggtatcaaa ttcattcgta aagagaaatt cctttaatga
     4561 agcagtatcg gcaaggtcaa tagctacaag acgaattgcc ttttcaaatt cctgatagcg
     4621 cctcggctct gtcttaggac gtactaaagc tgtaatatta aaatcgggac aggactttaa
     4681 catttcccgc aaaacatttc tgccaataaa accggtaatt ccggtaatta gaattttagc
     4741 caaaaatcct ctttattata ctgcgtctgt ggtgatgatg agaatgatga ggtcggtaaa
     4801 cctgaacata ttcataaggg atattttctc cgctggcaat gcaggaag
//
LOCUS       DAOPFL010000102         4825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_23597_length_4825_cov_91.734591, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000102 DAOPFL010000000
VERSION     DAOPFL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23597_length_4825_cov_91.734591"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..778
                     /gene="pstB"
                     /locus_tag="PLG69_06535"
     CDS             20..778
                     /gene="pstB"
                     /locus_tag="PLG69_06535"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPI43203.1"
                     /translation="MVNNILSTENLNLWFGRNYVLKYVNLNVLDKNITAVIGPSGCGK
                     STLLRCFNRMNDLIQDTHIEGKVKLLDDDIYTSKMDVTEIRSKIGMVFQKPNPFPKSI
                     YKNVAYGLQIQGKYHKDEINERVEQALKAAWLWEEVKDRLHSPAMALSGGQQQRLCIA
                     RALANRPEVLLLDEPTSALDPQSTSKIEELCLELKKSVCILIVTHNIAQAGRISNYTA
                     FMYLGELVEFGPTEKVFTVPADKRTESYLTGIFG"
     gene            800..1459
                     /gene="phoU"
                     /locus_tag="PLG69_06540"
     CDS             800..1459
                     /gene="phoU"
                     /locus_tag="PLG69_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPI43204.1"
                     /translation="MLIERISELKQLLMAEAGLVEKMILLAMDGLANFGTAFLEEVNT
                     FETRVNQIEIELDEKCLTVIALYQPEAKDLRQILMIYKINNDLERLGDQAVNIAESSA
                     QLVGSPVLQLIPELFAMKEAALKMLKDALFAFSGENYELSRQVCDADNIVDEYNRNIY
                     KHLVKLIRENPLQTDLYLHILRIAKNLERIGDLSTNIAEDTIFLSRGKVIKHRIDNIP
                     C"
     gene            complement(1620..2147)
                     /locus_tag="PLG69_06545"
     CDS             complement(1620..2147)
                     /locus_tag="PLG69_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPI43205.1"
                     /translation="MDNKKNNELITEYGNFAYGIAKLYRNKGLDWEDLKQEAILGLLK
                     AGEHYDPDKNVQFTTYATYWIKKQILAALKSSSFSYSEEIKENIGADNFPDKITDTNS
                     SNYEPAFVPISPNSIGFDFPEVMPALERTILQLSFGEGKSLKEIAHILQMSVERVTQQ
                     KRKALRRLKSLYPKE"
     gene            complement(2149..3483)
                     /locus_tag="PLG69_06550"
     CDS             complement(2149..3483)
                     /locus_tag="PLG69_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPI43206.1"
                     /translation="MAGKKNSIALLLSFSFVLLIGLMLILLLIQIPIWLLLIISLIIA
                     ALLTVNMIRILHQQFEQISQTAVQIANGNHSLRIPELETKEFCALGKDLNFMLEKLDK
                     TIYHLAVHREELRLVLSSIDDILWSQSSEGKLEWANEPFQKLFGNIGKKDNVFYTDII
                     RDPYLLSIIKDCGSSKDKLMKEIVMDNHNYLLSASHNDQAKRCIFILQNIDEIKQAEQ
                     MKKDFIVNLAHELRTPLTAISGFAEAMEDNINETNTRYLKIIQNHTQRLIHLISDLEQ
                     LIRLERTAAIELQVINLATFFANIAFILSPMVEEKGLYLKIELDEDLPYLTCDPFKLE
                     QVFINLVQNSLRYTITGGITIRSQKLKNEALFEVCDTGTGIDNIHLPRIFERFYVADP
                     ARNKTQSGTGLGLAIVKHIVQLHQGSITVQSELGKGSVFSVFLPLLQAPETH"
     gene            complement(3464..4180)
                     /locus_tag="PLG69_06555"
     CDS             complement(3464..4180)
                     /locus_tag="PLG69_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPI43207.1"
                     /translation="MDAKGKKIFVVEDETDILELIRMKLESAGFVTKGFKTALPMLNL
                     LKIEHPDLIILDLMLPDLDGMEVCLRIKKDKTTSAIPILMLTARTDLEDKIKGLEYGA
                     DDYVTKPFEGRELIARIKAILRRSEWENGKNALAITENFIIDFNHYEVWIEGKRIDLT
                     LTEFKILQLLTKRPGWVYNRSQILDYLWGNDKIVIERTIDVHIRNLREKLGAYAYLIR
                     NIRGVGYQFLCEEENGRKEE"
     gene            complement(4438..>4825)
                     /locus_tag="PLG69_06560"
     CDS             complement(4438..>4825)
                     /locus_tag="PLG69_06560"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HPI43208.1"
                     /translation="CATQNELNGDDARALINNNVFCVTEAANMPCTPEAMEIFLKAKI
                     LYAPGKAANAGGVATSGLEMTQNSIRMNWSREEVDEKLHGIMKNIHQACVDSGKEPDG
                     YINYVKGANIAGFLKVANAMCDQGIV"
BASE COUNT         1449 a          914 c          895 g         1567 t
ORIGIN      
        1 ccatcaggag aaaaagaaaa tggtaaataa cattctatca actgaaaatt taaacctatg
       61 gtttggtagg aactatgtgc ttaaatatgt taaccttaat gtattagaca agaatattac
      121 tgccgtgata ggaccttccg gctgcgggaa atctacttta ctgcgctgtt ttaaccggat
      181 gaatgatctt atacaggata cccatattga aggaaaagta aagcttttag atgatgatat
      241 ttatacttcc aaaatggatg taactgaaat tcgctccaaa attggaatgg tttttcaaaa
      301 acctaatccc tttcctaaga gcatttacaa aaatgttgcc tatggtttac agattcaagg
      361 taaatatcat aaagacgaaa ttaatgagag agttgaacaa gcattgaaag ctgcttggct
      421 ttgggaggaa gttaaagacc gtttgcatag tccggctatg gctctttccg gaggacagca
      481 acagcgttta tgtattgcca gagcattggc aaatagacct gaagttcttt tgctggatga
      541 accgacttcc gctttagacc ctcagtcaac ctcaaaaatt gaagaactgt gtttggaatt
      601 gaaaaaaagt gtttgcattt taattgtaac tcataatatt gctcaagcgg gtagaatttc
      661 caattatacc gcttttatgt atttgggaga attagtggaa ttcggaccca cggaaaaagt
      721 ttttaccgtg ccagcagata aaagaaccga atcctattta acaggtattt tcgggtaatt
      781 gctaaaataa ggagaaaata tgcttataga aagaatctcg gaactaaaac aattattgat
      841 ggcagaagcg ggattggtgg aaaaaatgat tcttttggct atggatggac ttgccaattt
      901 tggaaccgct tttctggaag aagtgaatac ttttgaaaca agagtaaatc aaattgaaat
      961 tgaattggac gagaaatgtt tgacggtgat tgcgttatat cagccggaag caaaagacct
     1021 gcgtcaaatt ctgatgattt ataaaataaa taacgatttg gaacgcttgg gcgatcaagc
     1081 agtaaatatt gctgaaagtt cggctcaatt ggtgggaagc ccagttttac aattgattcc
     1141 cgaactcttc gcgatgaaag aagcagcttt aaaaatgctg aaagatgccc tttttgcttt
     1201 ctcgggtgag aactatgaac tttcacgcca ggtttgcgat gccgataata ttgttgatga
     1261 gtataatcgt aacatttaca agcatttggt aaagctaatc cgagaaaatc cattacaaac
     1321 cgacctctat ctgcatatcc tgcgaatagc taaaaatttg gaaagaattg gcgatttatc
     1381 tactaatatt gctgaagata caatttttct gtcccgaggt aaagtaatta aacataggat
     1441 agacaacata ccttgctagt gtaaagataa aaaatttaag ctcggtaact tattgttcct
     1501 tggttatttg tttaacgact ctttaacact tctttaacac ttctttaaca cttccttaac
     1561 ttcgttaaag aatcgtttaa taatggttaa atgagtatta aagttagcag aaggagagtt
     1621 tactccttag gatacaagct tttcaggcga cgcaacgctt ttctcttttg ctgtgtaacg
     1681 cgttcaacgc tcatctgcaa aatgtgggca atttccttca aagattttcc ttccccgaaa
     1741 gatagctgaa gaattgttct ttccaaagcg ggcataactt cagggaaatc aaagccgata
     1801 ctatttggag aaataggtac aaacgcaggt tcgtaattac tgctatttgt atcagttatt
     1861 ttatccggaa aattgtctgc tcctatattt tcctttattt cttcagaata agagaatgaa
     1921 ctgcttttta gcgcagccag tatttgtttt ttaatccagt aagttgcata agtagtgaat
     1981 tgcacatttt tatccggatc ataatgttct ccggctttca gcaaacccaa gatagcttcc
     2041 tgtttcaggt cttcccagtc caatcctttg ttgcgatata atttagcaat tccgtaagca
     2101 aaattgccat attcggtaat tagttcattg ttctttttgt tatccaaatc aatgggtctc
     2161 aggtgcttgt aaaagcggta agaagacaga aaaaacgctg cctttgccga gttcactctg
     2221 cacggttata ctgccttgat gtaattgaac tatatgtttt acaattgcca agcccaatcc
     2281 tgttccactt tgggttttat ttctggcggg gtctgctaca taaaagcgtt caaaaatacg
     2341 aggtaaatgg atgttatcta tacctgtgcc ggtatcgcaa acctcaaaaa gggcttcatt
     2401 ttttagcttt tggctgcgga tggtgattcc acccgtgatt gtataacgca aagaattttg
     2461 gactaaattg atgaagacct gttccagttt gaaagggtca caagtgagat atggcaaatc
     2521 ttcatccagc tcaattttca ggtaaaggcc tttctcttcc accatcggac taaggataaa
     2581 ggcgatatta gcgaaaaagg tggctaagtt aattacttga agttcaatgg cagcggttct
     2641 ttccaggcga atcagctgtt ccaaatcgct aattagatga attaggcgct gagtgtgatt
     2701 ttggattatt tttaagtatc ttgtatttgt ctcattgata ttatcttcca tagcttccgc
     2761 aaaaccgctg atagcagtta aaggagttct tagttcgtga gccagattaa cgataaaatc
     2821 ctttttcatc tgttctgctt gctttatttc atctatattt tgaagaatga atatgcagcg
     2881 ttttgcctgg tcattgtgcg aagcggaaag caaataatta tgattatcca taactatttc
     2941 tttcatcagc ttatctttgc tacttccgca atccttgata atagacagca gataggggtc
     3001 tctgattata tcggtataga atacattgtc cttttttcca atattaccaa agagtttttg
     3061 aaagggctca tttgcccatt ccagtttgcc ttcggaactt tgagaccaca aaatatcatc
     3121 aatggaacta agcactaaac gcagttcttc acggtggaca gctaaatggt aaattgtttt
     3181 gtccaatttt tccagcataa aattcaggtc tttgcccaaa gcgcaaaatt cttttgtctc
     3241 caattcggga atgcgtaaag aatggtttcc gttcgcaatt tgcactgcgg tttgcgatat
     3301 ttgctcaaat tgctgatgca ggatgcgaat catattcacc gtaagtaatg cggcaataat
     3361 aagagatata attaataaaa gccaaattgg gatttgaatt aacagcagta tcagcatcaa
     3421 tcctatcaat agaacaaaag agaaagaaag taaaagagct atgctattct tctttccggc
     3481 cattttcttc ctcacagaga aattgataac ccacacctct gatgtttcta atcaggtagg
     3541 cataagcacc cagtttttct cttaaattgc gaatgtgaac atcaatagtg cgttcaatca
     3601 caatcttatc gttcccccaa agataatcca aaatctgact gcggttataa acccacccgg
     3661 gtcttttagt aagtaactgc aggattttaa attccgttaa agtaaggtct atccgcttac
     3721 cttctatcca cacctcatag tgattaaaat ctataataaa attttcagta atggctaacg
     3781 catttttgcc gttttcccac tcactccgtc gtaaaattgc tttaattctg gcaataagtt
     3841 ctctaccttc aaagggtttg gttacataat catcggctcc gtattccaag cccttgattt
     3901 tatcttccag gtccgttctt gctgttaaca tcaaaatagg aattgcggag gttgttttat
     3961 ctttttttat tctcaaacaa acctccatac catctaaatc cggcaacatc aaatccagaa
     4021 taatcaggtc aggatgttca attttaagca aattgagcat tggtagggct gttttaaaac
     4081 cctttgtaac aaatcctgca ctctccagtt tcattctgat gagttccaga atgtctgttt
     4141 catcctcaac tacgaatatc tttttacctt ttgcatccat ataaaacctt taaataatat
     4201 aactgatttt gagcaagtaa aaacagccgg gttttggtta aattttctta acaataagtt
     4261 gctgaggttt ttaaggtctg gacgaaattt ggtttgcaac gatatttatc tccttgtttc
     4321 accttttccc ctttttttac ataatatgct ttccagttta ctcattaacc taaaaaagcc
     4381 cgttacgcaa gaatcgtaac gggctttatg tttatcagag agattagggg tttttcttta
     4441 cactatacct tggtcacaca ttgcattggc aactttcaag aaaccggcaa tattagcacc
     4501 tttcacataa ttaatatagc catcgggttc tttgccacta tccacacatg cctgatgaat
     4561 gtttttcata attccgtgca gtttttcatc cacttcttct ctgctccaat tcatacggat
     4621 ggagttttgc gtcatttcca gtccgcttgt agcaactcca ccggcatttg cagctttgcc
     4681 tggagcataa agaatttttg ctttcaggaa aatttccatt gcttcaggag tacaaggcat
     4741 attagcagct tcggttacac aaaatacatt gttattaata agtgccctgg catcatctcc
     4801 attgagctca ttttgcgtag cacaa
//
LOCUS       DAOPFL010000103         4774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_23949_length_4774_cov_107.262344, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000103 DAOPFL010000000
VERSION     DAOPFL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23949_length_4774_cov_107.262344"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLG69_06565"
     CDS             <1..204
                     /locus_tag="PLG69_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPI43209.1"
                     /translation="GLIVDYDPDKSSLKAQLKSADTSGAKYAIIIGENELATKTVNLK
                     NLQTGEQIAIPMDEEYTINKMKN"
     gene            complement(298..1650)
                     /locus_tag="PLG69_06570"
     CDS             complement(298..1650)
                     /locus_tag="PLG69_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPI43210.1"
                     /translation="MIKPVIFRQYDIRGIVNEELNEESYYLIGKGFGTYLRNKGLKSI
                     VLGGDARHSTPLFMNAFSKGALETGCDIIDLGIVPTPVLYFSIWKLQSDGGAMVTASH
                     NPSQYNGCKLNLGLSSVYADELQKVLQIIQKGDFATGNGKLVKNNEIGNVYIDYIVNG
                     IKLQRPVKVIVDGGNGVGGPYLTTILRLLGCEVIDMFCEPDGDFPNHHPDPTIAKNME
                     ALSKAVVDNKYELGIGLDGDADRIGVIDEQGKLLLGDQILNILARDYLKHNPGKKIIA
                     DVKCSMNLFNDIKKYGGEPIMYKTGHANIKMFMKEMGVEFAGEMSGHIFLADRYLGFD
                     DAIYVSCRFVEIVSQTHNPVSTFLADQPKLYNTPELHTNCPDEKKFEVVAKVCAEFQK
                     EGYDVNDIDGARITFPDGWGLIRASNTTPVIVTRYEALTESRMNEIKNLIESKINKYL
                     "
     gene            complement(1665..2255)
                     /gene="rdgB"
                     /locus_tag="PLG69_06575"
     CDS             complement(1665..2255)
                     /gene="rdgB"
                     /locus_tag="PLG69_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPI43211.1"
                     /translation="MIRDILIATHNPDKLKELEELVNPLKLRLHFLRELPGFYPTEED
                     QETLQKNAMKKALEAAKYSGLITLADDTGLFIEALNGAPGIMSARFAGNSCSYSDNRQ
                     KVLRLLQNIQIRKAYFKTAVALSAPDGIIAVTEGRLEGEITTEERGNNGFGYDSIFAV
                     NGKTYAEMNADEKNHLSHRALAIKSIIPILQKIINN"
     gene            complement(2252..2779)
                     /locus_tag="PLG69_06580"
     CDS             complement(2252..2779)
                     /locus_tag="PLG69_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HPI43212.1"
                     /translation="MKDDDVIEGREIPAEEQEKIKDKIIDDIDSTKLKFYENLRQKVR
                     GWTKEQLGSLGGKLGEYVFLLPDFFILVCRLALDKRVPVKQKMIMGGVVAYVMMPVDL
                     IPDFIPVIGLVDDLVLVVMALNMILNEIDPKILSDNWSGEGEVLEHLKKITAVAEQFL
                     DKHLLQKIKNVLKRL"
     gene            complement(2997..>4774)
                     /gene="ppdK"
                     /locus_tag="PLG69_06585"
     CDS             complement(2997..>4774)
                     /gene="ppdK"
                     /locus_tag="PLG69_06585"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPI43213.1"
                     /translation="ISEEERLSKYPSLEEAMPETYKELFEIQKRLENHYRDMQDMEFT
                     IQEGKLWLLQTRTGKRTGLAMIKIAMDMLREGMIDEKTALKRIDPAKLDELLHPVFTK
                     EGLAKAKVIANGLPASPGAASGQIVFFADEAEAWAADKKKVILVREETSPEDLRGMAV
                     AQGILTARGGMTSHAAVVARGMGKCCVAGAGALQIDYKARTMTVNGKVYKEGDWISLN
                     GSTGQILEGKIDTQDPELSGDFGAVMQLADKYTKMKVRTNADTPKDATVARNFGAQGI
                     GLCRTEHMFFEGERIKAMREMILAEDETGRKKALAKLLPMQKTDFEGIYTAMNGFPVT
                     IRLLDPPLHEFVPQEESAQKDIAEELGIPLEKVKARVASLHEVNPMLGHRGCRLGNTY
                     PEITEMQTRAIIEAALAVKARGISVLPEIMVPLTSTAEEFEMQEDIIRKTAEKVFAEK
                     QDRVDYLVGTMIEIPRAALTADKIAEHAEFFSFGTNDLTQMTFGFSRDDAGVFLPVYL
                     EKNLLKHDPFQIIDREGVGQLVKMGTERGRKTRPDLKVGICGEHGGEPSSVEFCHLVG
                     MNYVSCSPYRVPIARVAAAIAALND"
BASE COUNT         1376 a         1063 c          820 g         1515 t
ORIGIN      
        1 ggacttattg tggattacga tcccgataaa agctctctga aagcccaatt aaaatccgcc
       61 gataccagtg gagcaaaata tgctataatc atcggggaaa atgaacttgc cactaaaaca
      121 gtgaacctta aaaacctgca aaccggtgaa caaatagcta ttccgatgga tgaggaatac
      181 actatcaaca aaatgaagaa ttaaatgggt tgctaaattc cgattcggca aaaggtttca
      241 gggatgtttt tttagcggtc tttgacctct ttagcaaatc ggaatttgcc aaccccctta
      301 caagtatttg ttgattttgg attctatcag gttctttatt tcattcattc tactttcggt
      361 taatgcttca taacgggtaa caattacagg tgtggtattg gaagcacgaa ttaaacccca
      421 gccatcagga aaagttattc tggcaccatc aatatcatta acatcatagc cctctttttg
      481 gaattctgca cagacctttg ctaccacctc aaactttttc tcatcgggac aattggtatg
      541 cagttcagga gtattataaa gttttggctg atccgctaaa aaagtgctga ccggattatg
      601 agtttgagaa acaatttcta caaaacggca actgacatag atggcatcat caaaacccaa
      661 atatctatct gccaaaaaga tatgtccact catttcacct gcaaattcca ctcccatttc
      721 tttcataaac attttgatat tggcatgacc tgttttatac ataatcggtt ctccgccata
      781 ctttttgatg tcattaaaca gattcatact gcattttaca tcggcaataa tctttttccc
      841 ggggttatgc ttcaaataat ccctggctaa gatattcagt atttggtcac ctaacaatag
      901 tttaccttgt tcatcaataa ctccaattct atctgcatcg ccatccaaac caatgccaag
      961 ttcatattta ttatcaacca ctgcctttga tagcgcttcc atattttttg ctattgtagg
     1021 atcgggatga tgattgggaa aatcaccatc gggctcacaa aacatatcta tcacctcaca
     1081 acccaaaaga cgcaaaatgg tagttaaata aggaccacca actccatttc ctccatctac
     1141 aatcactttt accggacgct gaagtttgat accattaacg atatagtcaa tgtaaacatt
     1201 gcctatttcg ttgtttttta ccaactttcc attgccagtg gcaaaatcac ccttttgaat
     1261 gatttgtagt accttttgca gttcatcagc ataaacactg ctcaagccaa gatttaattt
     1321 acaaccattg tattgagaag gattatgact tgccgtaacc attgctcctc catcactttg
     1381 cagtttccag atggaaaaat acaaaaccgg agtaggaaca atgcctaagt caataatatc
     1441 acaaccggtt tccaaagcac ctttgctaaa ggcattcata aaaaggggag tagaatgtct
     1501 ggcatctcca cctaagacaa tacttttcag acccttattt cgcaagtagg ttccaaagcc
     1561 cttgccaatc aggtaatatg attcttcatt cagttcttca tttacaatac cacgaatgtc
     1621 gtattgacga aagattaccg gttttatcat tgataactcc tttttcagtt attgattatc
     1681 ttttgtaaga tagggataat agattttatg gctaaagcac gatgggaaag gtggtttttt
     1741 tcatccgcat tcatttcagc gtaggtttta ccatttacag caaaaatgct gtcataacca
     1801 aaaccgttat tgccacgctc ttctgttgtt atttcaccct ctaatctacc ttctgtaaca
     1861 gcaataatgc catcaggagc tgataaagca actgctgttt taaaatatgc tttccgaatt
     1921 tggatgtttt gtagaagccg taacaccttt tgcctattat cagaatagct gcaggaattt
     1981 cccgcaaaac gagctgacat aattcctggt gccccattta aggcctcaat aaaaagtcct
     2041 gtatcatctg ccaaagtaat tagaccgcta tattttgctg cttccaatgc ttttttcatt
     2101 gcattttttt gcaaggtctc ctgatcttcc tctgtaggat aaaatccggg cagttcacgc
     2161 aaaaaatgca aacggagctt taagggattt accaattcct ccaattcttt cagcttatca
     2221 ggattgtgag tggcaattaa tatatcccga atcataagcg ttttagcaca tttttgattt
     2281 tctgcagtag gtgtttatcc aaaaattgtt ctgccacagc ggtaatcttt tttaagtgtt
     2341 ccaaaacttc accttcgcca ctccagttat cggaaagaat tttgggatca atttcattca
     2401 aaatcatatt gagtgccatc acaacaagca ctaaatcatc taccagacca attacgggaa
     2461 taaaatccgg aataagatca accggcatca taacataagc aacaactccg cccataatca
     2521 ttttttgttt tacagggact cgtttatcca gtgccaaacg acaaactaaa atgaagaaat
     2581 ccggtaacag gaaaacatat tcacccaatt ttccacccag ggaacccaat tgttcttttg
     2641 tccaacccct aactttttgt ctcaggttct cataaaattt cagtttggtg gaatcaatgt
     2701 cgtcaattat tttatcctta attttttcct gttcttccgc agggatttct ctgccttcaa
     2761 taacatcgtc atctttcata ataaacctcc atctgcctat aatttaatag taatattgaa
     2821 gtcaagcaaa ttatttgagg tagagatgtc aagaagtcaa aagatttgtg tagtcggagc
     2881 ttgctgaagc ttagcatttt ttcaagttca gatattttgc ggaggaacgg tgtcccctga
     2941 ctacaaaaaa gcggaggaac ggtgtcctcc gcttatatat aaattgtttt ccaggattaa
     3001 tcgtttaaag cagctatagc agcagcgaca cgagcaatag gaactctata aggagaacag
     3061 ctaacatagt tcattcccac taagtggcaa aattccacac tgcttggttc tccaccatgt
     3121 tctccgcaaa ttccaacttt taaatcaggt ctggttttgc gtccgcgttc agttcccatt
     3181 ttcactaatt ggcctacacc ttctctgtct ataatttgga aggggtcatg tttcagcaga
     3241 tttttttcca aatacacggg taaaaacacg cctgcatcgt cgcgtgaaaa accaaatgtc
     3301 atttgagtta aatcatttgt tccaaagctg aaaaactcgg catgttcagc aattttatcg
     3361 gcagttaagg cggcgcgggg aatttctatc attgttccta ctaagtaatc cacgcgatcc
     3421 tgcttttctg caaagacctt ttctgctgtt ttgcggataa tatcttcctg catttcaaat
     3481 tcttcagcgg tgctggtaag cggaaccata atttcaggta atacactaat tccgcgtgct
     3541 ttaacagcta aagcggcttc aattattgca cgagtttgca tttctgtaat ttctggatag
     3601 gtatttccca aacggcaacc tctatgacca agcataggat ttacttcatg taacgaagca
     3661 acgcgtgctt taactttttc cagaggaatt cccagttctt cggcaatatc cttttgagcg
     3721 gattcttcct gaggaacaaa ttcatgcaag ggggggtcta aaagacggat agttacagga
     3781 aatccgttca tagcggtata aatgccttca aaatcagttt tttgcatagg tagtaatttt
     3841 gccaatgctt ttttacgtcc agtttcatct tccgctaaaa tcatttcccg cattgcctta
     3901 attctttcgc cttcaaaaaa catatgttcc gttctgcaaa gtccaattcc ctgagcacca
     3961 aaatttctgg caacggtagc atctttggga gtatctgcat tggtgcggac tttcattttg
     4021 gtatatttat cggcaagttg cataacagca ccaaaatcac cgctaagttc agggtcttgg
     4081 gtatctattt taccttctaa aatctgtcct gtagagccat ttaaggaaat ccaatcccct
     4141 tctttataga cctttccatt cactgtcata gttctggctt tgtaatcaat ttgtaatgcc
     4201 ccagctcctg ccacacaaca ttttcccatt cctctggcaa caacagctgc atgagaagtc
     4261 atacctccgc gagcagttag gattccttgt gcaactgcca ttccgcgtaa atcctccggg
     4321 gaggtctctt ctctaactaa aataactttt ttcttatcag cagcccaggc ttctgcttcg
     4381 tccgcaaaaa acacaatttg tccggaagcc gctccaggag aagcaggcaa accattagca
     4441 atcacttttg cttttgccaa accttctttt gtaaagacgg gatgcagaag ttcatccaat
     4501 ttagcaggat caattctttt aagagcggtt ttttcatcaa tcataccttc gcggagcata
     4561 tccattgcga ttttaatcat agcaagaccg gttcttttgc cggttcgggt ttgtaagagc
     4621 cacaattttc cttcctgaat ggtaaattcc atatcttgca tatcgcgata atgattttct
     4681 aaccttttct gaatttcaaa gagctcttta taggtctcgg gcattgcttc ttccagagaa
     4741 ggatatttac ttaagcgttc ttcttcactg atac
//
LOCUS       DAOPFL010000104         4716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_24356_length_4716_cov_83.507402, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000104 DAOPFL010000000
VERSION     DAOPFL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24356_length_4716_cov_83.507402"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLG69_06590"
     CDS             complement(<1..644)
                     /locus_tag="PLG69_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPI43214.1"
                     /translation="MKKTLLVIVLLLTLSLLLALPAEFTRAGNPELYKELSAKAKNNL
                     KHLTLEQKQQYKDLLKQYPDILMNYLLAYESDSNLAEAKPEDVKSNYEMICALLNKKG
                     LKYPVDFFLSYIAKQTVSDEPLTAYRKILLQDGLQNIINTTNSDLEMFRAVTLWCVSR
                     IQFQQTSGRDQSPLDIVQKSLIGRCEEMQILLVSACRTIGLPARPAYTPWWAHT"
     gene            complement(880..3453)
                     /locus_tag="PLG69_06595"
     CDS             complement(880..3453)
                     /locus_tag="PLG69_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPI43215.1"
                     /translation="MIVRYEDKKDLGKKEEVKQQKQQIEDFIRAFKQEKRFASTIVAE
                     NKEPAKAAQTDFYPEGLLPPVQNLLQRMGIEKPYIHQAEAIKALLAGKNVVVSAGVAS
                     GKSLVYQAVILNSLVQNPQSRALLLFPTKALAQDQAQKMQMLCKELNTQNPALPAIVN
                     GIYDGDTATDLRGKLRKQANILFTNPDMLHLGILPNHTLWSAFFSRLAYVIIDEVHIY
                     RGVFGSHTANLFRRLKRITAVYGIKPQFIFTSATLANARELAETLIEEPVVLIDKDGS
                     PKGERHFYICNPPVIEPALGIRRSSTQEISSIAKSLLQTNLQALLFTGSRRSVELIFR
                     YLTSDRKIADKVRSYRSGYLAEERRKVERDLREGEIGLVISTNALELGIDIGGLDAIL
                     LNGYPGTICATRQQAGRAGRKGNPSLCILEASGNPLDQYICQHPEYIFANNPEQALLD
                     PNNSEILRLQLLCAISEMAFKEGENFGALSFAEIQGHLFALLDEGLIRHIGNRYIGIS
                     GKYPAGDVSLRNAGNQFQILADDELVGWVDSGSVKWMTHPNAIYLHQGETWVVKELSI
                     EQKKVILEPVQVNYYTQATQFTEIALNNLLRLENVTGGRKHFGEVTVTRTITGFKRLR
                     FWTMEVLDQEELDLPPEIMQTQAYWISLAEETVERIRNQGLWNNDRNDYGPKWEEICE
                     QIRQRDNYHCRNCGATGDLEVHHIIPFRRFEDPDEANEPDNLVALCPRCHRLAETRVH
                     IQSGLAALAYLLGNLAPFFVMCAPQDLGVHSEDKSPLALGNPVIVIYDNFPGGIGLSR
                     KLYELHNQLLYAGMDRIKGCACENGCPACVGPVAENGIGAKEEAMAILKELIEEGRIE
                     N"
     gene            complement(3879..3955)
                     /locus_tag="PLG69_06600"
     tRNA            complement(3879..3955)
                     /locus_tag="PLG69_06600"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3919..3921),aa:Val,seq:cac)
     gene            complement(4049..4125)
                     /locus_tag="PLG69_06605"
     tRNA            complement(4049..4125)
                     /locus_tag="PLG69_06605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4089..4091),aa:Val,seq:gac)
     gene            complement(4208..>4716)
                     /locus_tag="PLG69_06610"
     CDS             complement(4208..>4716)
                     /locus_tag="PLG69_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43216.1"
                     /translation="APADWYFNYCDETGNCYPGSIDRPFSMSAGEALSFHLNLTVGSS
                     AIVDFRFVVSSPHIGSYSIPFRYDAGTPVIDETNNPGALMINNLYPNPVSGTAVFRLL
                     SPKNNDSATIEIYNVKGQKVQCLTAINLHNGINEISFVPDSSLPNGVYFYRLQNTNSK
                     VGRFLLLQ"
BASE COUNT         1367 a         1033 c          892 g         1424 t
ORIGIN      
        1 tccgtatgag cccaccaagg tgtatatgca ggacgggcag gaagtccaat tgttcggcag
       61 gcggaaacaa gtaaaatttg catttcttca cagcgtccga ttaaggactt ttgcacaata
      121 tccaaaggac tttgatctct tcccgaggtc tgttgaaact gaattctgga aacacaccag
      181 agagttacgg cacgaaacat ttccaaatcg gaattggtag tattgattat attctgcaga
      241 ccgtcttgca ataatatttt ccgataagcc gtaaggggtt catcagaaac ggtttgtttg
      301 gctatatagg aaaggaaaaa gtccacagga tatttcagtc cttttttatt cagcagtgca
      361 caaatcattt cataattgct ttttacatct tccggttttg cttctgccaa attactgtca
      421 ctttcataag caagcagata gttcatcaaa atatcgggat attgtttcag caggtctttg
      481 tattgctgtt tttgctccaa agttaaatgc ttcaaattgt tttttgcctt tgcagaaagt
      541 tctttgtaga gttccggatt tccagcacgg gtaaattctg caggaagagc taataacaat
      601 gaaagcgtga ggagtagaac aatgactaat aatgtttttt tcacctctcg ccccttttac
      661 ttttttttct gggcattatt ttctttttat acataaaata agacaaaatt aaatgcaggc
      721 aagaaaaagc tatagaatga agaattgaga attgagaatg attgaagtca ggggtcattc
      781 ctcccagtaa aaattataaa catcccacga tttcgtttgg cagtaaggac tcgtgacggc
      841 ataaggaaaa aatttgtgaa gatgctaata aatcctgatt taattctcaa ttcttccttc
      901 ttctatcagt tcttttagga tagccatcgc ttcttctttt gctcctatcc cattttccgc
      961 tacaggaccc acacaggcag gacaaccatt ttcgcaggca caacccttaa ttctatccat
     1021 tccggcatac aaaagttgat tatgcagttc atacaatttt cggctaagac ctattccccc
     1081 gggaaaatta tcgtaaataa caattacagg attgccaagt gccagaggag atttatcttc
     1141 actatgaact cccaaatcct gaggagcgca cattacaaaa aacggtgcca aattaccaag
     1201 cagatatgcc aaagcagcta atccgctttg aatatgcact ctggtttctg ccaaacggtg
     1261 acagcgtggg cataaagcta caagattatc cggctcattt gcttcatctg ggtcttcaaa
     1321 acggcgaaaa ggaattatat gatgcacttc caaatcccct gttgcaccac aattccggca
     1381 gtgataatta tcgcgctggc ggatttgttc acaaatttct tcccatttag gcccataatc
     1441 atttctatcg ttattccaca aaccctgatt gcgtattctt tctaccgttt cttctgcaag
     1501 ggaaatccaa tatgcctggg tctgcataat ttcgggtgga aggtcaagtt cctcttggtc
     1561 taaaacctcc attgtccaaa accgcaagcg tttaaagccg gttatagttc tggtaactgt
     1621 cacttctcca aagtgtttac gtcctcctgt aacattttcc aggcgcaaca gattgtttaa
     1681 agcaatttca gtgaattgtg ttgcctgcgt gtaataattt acctgaacgg gctctaaaat
     1741 cacttttttc tgttctatac tcagttcttt tactacccag gtttcaccct gatgaaggta
     1801 aatagcattg ggatgagtca tccatttcac gcttccggaa tctacccaac caactaattc
     1861 gtcatctgcc agaatttgaa attgatttcc cgcattgcgt aaagaaacat ccccggcagg
     1921 atattttccg cttattccta tatagcggtt tccgatatgc cttattaagc cctcatccaa
     1981 aagggcaaaa aggtgaccct gaatttcggc aaaacttaag gcaccgaagt tttctccctc
     2041 cttaaaagcc atttcactga tggcacaaag cagttgcaga cgcaaaatct cactgttatt
     2101 agggtctaat aatgcctgtt caggattgtt ggcaaaaata tattcagggt gctgacagat
     2161 atattggtct aaaggatttc cgctggcttc caaaatacaa agagatggat ttccctttct
     2221 tccagctcta ccggcttgtt gtctggtggc acaaattgtt ccgggatagc catttaaaag
     2281 gatagcatcc agtcctccga tatcaattcc cagctctaag gcgttggtag aaataactaa
     2341 accgatttct ccttcccgta agtccctttc tacttttcgg cgttcttcag ccaaataacc
     2401 gcttctgtaa ctgcgaactt tatccgctat tttccgatca gaggttaaat aacgaaaaat
     2461 gagttccaca ctgcgtcggc tgcccgtaaa aagaagtgcc tgcaaattcg tttgtaataa
     2521 acttttggca atactgctta tttcctgggt agaacttctc cggatgccta aagcgggctc
     2581 aataactggt ggattgcaaa tataaaaatg cctttctccc ttgggtgaac catctttatc
     2641 tatcagcaca accggttctt caataagggt ttcggcaagt tcacgcgcat tggctaaagt
     2701 agcggaagtg aaaataaact gcggttttat gccataaaca gccgtgatgc gtttcaaacg
     2761 cctgaacaaa tttgccgtat gcgaaccgaa aacaccacga taaatatgca cttcatcaat
     2821 tatcacataa gctaaacggg agaaaaatgc gctccataaa gtatgattcg gtaaaatacc
     2881 caaatgcaac atatcaggat tggtaaaaag aatatttgcc tgtttgcgta gttttccgcg
     2941 taaatccgtt gccgtatcgc catcgtaaat gccattgaca attgccggta aggcaggatt
     3001 ttgtgtgttc agctctttac aaagcatctg cattttctgc gcctgatcct gtgccaatgc
     3061 ctttgtcgga aaaagaagca aagctctact ctgcggattt tgcaccaaac tgtttaagat
     3121 aactgcctga taaactaaac ttttcccgct ggcaaccccg gcactaacca ctacattttt
     3181 ccccgctaaa agagctttaa ttgcctctgc ctgatgaata tagggctttt ctatgcccat
     3241 cctttgcagt aaattctgca caggaggtaa aagaccttca ggataaaaat ccgtttgcgc
     3301 tgccttagcc ggctctttat tttccgctac aattgtagag gcaaaacgct tttcctgctt
     3361 aaaagcccga ataaagtcct ctatttgttg cttttgctgt ttcacttctt cctttttccc
     3421 caagtccttt ttatcttcat accttactat cattatggca gttacggtca agcataatct
     3481 taaacctaaa aaaagcggcg gaaggaaata gagttggtaa attctttctt ggctaaagca
     3541 accgaaggtt tctcaagaaa tttgctttca gcattttccg acttggaatt tggcaaccca
     3601 atttctcaat ttgtgtatta ttcattttta ttgccttatt ttggtataaa catatatcta
     3661 tttgtattat aagctgttat accgttttct cctgtcttcc ctaatagcat tgcttaccca
     3721 ttgcttaccc attacggatg gcattagcaa tgagtccgta ttgagtaagc aatgcttcct
     3781 gcaaagctgt agacggagga caccgttcct ataccagaaa aatggtttag ctggttgaga
     3841 ttctctctgc cctccgctct ctgccctctg ccaaaaagtg gtgggtgata caggattcga
     3901 accagtgacc tctacgatgt gaacgtagcg ctctaaccag ctgagctaat cacccttgca
     3961 cctttaaaat gtaaaaatat tatataagag aatgtctcaa aagacaaaat aatgagctaa
     4021 ccacataact atcagtaaca aaagaagatg gtgggagata ctggattcga accagtgacc
     4081 tctacgatgt caacgtagcg ctctaaccaa ctgagctaat cacccactct ttagtcagaa
     4141 ttcttaaatg gtcttttttg tcaagttccg aattgtgtat aagcttaaaa aggcgttata
     4201 atggctttta ctgcaagagg agaaagcgtc ctaccttgct gtttgtgttt tgcaagcgat
     4261 aaaaataaac cccgttggga agagaagaat ccggaacaaa gctgatttca tttatgccat
     4321 tatgcagatt aatagcagtt aggcactgca ctttttgtcc tttcacatta taaatttcta
     4381 tcgttgcaga gtcattattt ttaggactta aaagacggaa aaccgcagtt ccggaaacgg
     4441 gattgggata taaattatta atcatcaagg cacctgggtt attagtttca tctatcacag
     4501 gcgtgccagc atcataacga aaaggaatgc tgtaacttcc aatatgagga ctactgacta
     4561 caaaacggaa gtcaactatg gcagatgagc caaccgttaa attcagatga aaggataaag
     4621 cttctcctgc agacattgaa aaaggacggt caatgcttcc tggatagcaa tttcccgttt
     4681 catcacaata attaaaatac cagtccgctg gtgcat
//
LOCUS       DAOPFL010000105         4671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_24690_length_4671_cov_79.437175, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000105 DAOPFL010000000
VERSION     DAOPFL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24690_length_4671_cov_79.437175"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1368
                     /gene="tuf"
                     /locus_tag="PLG69_06615"
     CDS             184..1368
                     /gene="tuf"
                     /locus_tag="PLG69_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPI43217.1"
                     /translation="MAKEKYVRTKPHLNVGTIGHIDHGKTTLTAAITLYLSKKGGAKF
                     RTFDSIDNAPEEKARGITIATAHVEYETEKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVSADDGPMPQTREHILLARQVGVPAIVVFMNKCDLVDDPELLDLVEMEVRELLDK
                     YEFPGDEVPVIRGSALKALNGDPEAEKQIQALLDAVDSYIPLPERPIDKPFLMPVEDV
                     FSIPGRGTVATGRVERGVIKVGDKVERVGIRETVETTCTGVEMFRKLLDEAQAGDNIG
                     VLLRGFGKKDVVRGMVLAKPKSITPHTKFIGQTYVLTEEEGGRHKPFQSGYRPQFYFR
                     TTDVTGSLYLPEGVKMVMPGDNVEIQAELITPIAMEQGLRFAIREGGRTIGSGVVSKI
                     IE"
     gene            1388..1534
                     /gene="rpmG"
                     /locus_tag="PLG69_06620"
     CDS             1388..1534
                     /gene="rpmG"
                     /locus_tag="PLG69_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPI43218.1"
                     /translation="MRDIIILACEECKNRNYTTTRNKRKHPTRLELKKYCPTCRKHTV
                     HKQR"
     gene            1560..1635
                     /locus_tag="PLG69_06625"
     tRNA            1560..1635
                     /locus_tag="PLG69_06625"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1593..1595,aa:Trp,seq:cca)
     gene            1703..1888
                     /gene="secE"
                     /locus_tag="PLG69_06630"
     CDS             1703..1888
                     /gene="secE"
                     /locus_tag="PLG69_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPI43219.1"
                     /translation="MKFEKITRFFRDVRSEMKCVSWPTKTDLKEGTLVVIIMSAIVAI
                     FLSLVDFGFTKIVELIF"
     gene            1906..2439
                     /gene="nusG"
                     /locus_tag="PLG69_06635"
     CDS             1906..2439
                     /gene="nusG"
                     /locus_tag="PLG69_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPI43220.1"
                     /translation="MKWYVIHTYSGHENKVKTAIEKGLAGTPLEKQVGRLLVPVRKTF
                     VIREGKKIERDKKLFTSYVIMEADMTPELKTYILQIAGVTSFLGINKEHKEPIPLSQE
                     EVDRLLGITDPNRDIKVYSYMPGDMVKVISGPFTDFEGIVQKTTENGTKLIIEVTVFG
                     RRTPVELNASQVELIKK"
     gene            2464..2895
                     /gene="rplK"
                     /locus_tag="PLG69_06640"
     CDS             2464..2895
                     /gene="rplK"
                     /locus_tag="PLG69_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPI43221.1"
                     /translation="MAKPKDAVATLKLQLPAGKATPAPPVGPALGQVGVNIPEFCRQF
                     NEKTADQPGMVFPVIIYVAKNKSFTFEIKTPPAAVLIKKEAGLATGSATPNKLKVGKI
                     TNEQLKNIAQLKIKDMNCNSLESAMSMIAGTARSMGVTIED"
     gene            2937..3641
                     /gene="rplA"
                     /locus_tag="PLG69_06645"
     CDS             2937..3641
                     /gene="rplA"
                     /locus_tag="PLG69_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPI43222.1"
                     /translation="MKHSKRYNVAYTMFDRQKRFDLNEAIKLLKSLPTSKFDETVELH
                     FNLGVDPRKADQQIRNSLVLPHGTGKEMRVLVFAEGDKAEEAKKAGADYVGVDDLIEK
                     ISNGWFDFDVVIATPNLMGRIGKLGRVLGPRGLMPNPKVGTVTMDVGKAVQEAKSGKI
                     TYRVDKFANLHIPVGKLSFEEEKLKDNIKAVLAAVLRDRPPTLKGVYIKSITLCTTMS
                     PGIKIQVASATAEARS"
     gene            3654..4190
                     /gene="rplJ"
                     /locus_tag="PLG69_06650"
     CDS             3654..4190
                     /gene="rplJ"
                     /locus_tag="PLG69_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPI43223.1"
                     /translation="MVQSIKYKIVKDLTERLSGARAIVLVDYKGINIEQVNQLRNRFR
                     ESQVDYFVQKNTLIKIALNDLGITELDPYLKGPTAVAVSKLDEVSPARELIKFLKEVM
                     EDKGFPSFKVGYIAGHIFNADELTALAKLPSREELLAKVLAGMNAPLSNFVSINQGIL
                     RKFVYALDAIAKKQAEAS"
     gene            4223..4606
                     /gene="rplL"
                     /locus_tag="PLG69_06655"
     CDS             4223..4606
                     /gene="rplL"
                     /locus_tag="PLG69_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPI43224.1"
                     /translation="MSDKKQQVIDLIKEMTVLELSELVKEMEEIFGVSASAPVAAFMP
                     TQGSAAEGGEKEEQTEFDVILTSAGDKKINVIKVVREITKLGLKEAKDLVDGAPKVVS
                     EKVSKEEAESIRKKLEEAGATVEVK"
BASE COUNT         1541 a          831 c         1066 g         1233 t
ORIGIN      
        1 acctccttgg taaggaggag gtcaccggtt caagtccggt cttgagctcc acttttgaag
       61 ttaaagagat gtttttaatt tcaacatcta tgatattaag aggaatatat acctgaaggt
      121 gtatattttt taataaatta agaaaacgag acgggcttgt gtgattcaag tccaggagga
      181 acaatggcaa aagagaaata tgttagaact aaaccacatc tgaatgtggg aacgattggt
      241 catattgacc atggaaaaac aacgctaact gcagcgatta ctttgtatct gagtaaaaaa
      301 ggcggagcca aattccgtac ttttgatagt attgataatg ctccggaaga aaaagcacgc
      361 ggaataacta ttgctacagc ccatgtagaa tatgaaacag aaaaacgtca ttacgctcat
      421 attgactgtc ctggtcatgc tgactacatt aaaaatatga taactggtgc agctcaaatg
      481 gatggtgcca ttttggttgt gagtgctgat gatggaccta tgccacaaac ccgcgagcat
      541 attttgcttg ctcgtcaggt tggtgttcca gcaatagttg tatttatgaa caaatgcgac
      601 ctggttgacg accctgaatt gcttgatctg gtggaaatgg aagttcgtga attgcttgat
      661 aagtatgaat ttccgggtga tgaagttccc gtaattcgcg gttctgctct taaagcactc
      721 aatggagatc ccgaagctga aaaacaaatt caagctcttc tggatgcggt agatagctat
      781 attccgcttc ccgaacgtcc aattgataag cccttcctga tgcctgtgga agatgttttc
      841 agtattccag gtcggggaac cgttgccaca ggacgtgtag aaagaggtgt tatcaaagtt
      901 ggtgataaag ttgaacgcgt tggaattaga gaaacagtgg aaacaacctg cacgggcgtg
      961 gaaatgttcc gtaaactgct tgacgaggca caagccggag ataatattgg tgttctttta
     1021 cgtggattcg gcaaaaaaga tgtagtgcgt ggaatggtgc tggcaaaacc caaatccatt
     1081 actcctcata ctaaattcat aggtcagacc tatgtattaa ccgaggaaga aggtggacgc
     1141 cataaaccct tccaaagtgg gtatcgtcct caattttatt ttcgcactac ggatgttacc
     1201 ggaagcttat atcttcccga aggagtgaaa atggttatgc ctggtgacaa tgtagaaatc
     1261 caagctgaac tcataactcc tatcgctatg gaacaaggtc tgcgtttcgc tatccgcgaa
     1321 ggtggaagaa ccatcggcag tggtgttgtc tctaaaataa tagaataaga gaagcggaca
     1381 aacaataatg agagatataa ttatccttgc ctgtgaagaa tgcaagaatc gtaattatac
     1441 aactacccgt aataaaagaa agcatccgac acgactggag ctaaaaaaat attgccccac
     1501 ctgccggaaa catactgttc ataaacagcg ttagtaattt accctaaaaa atgttgcaca
     1561 ggacagtagt tcaactggta gagcaccggt ctccaaaacc gggggttgcg ggttcgattc
     1621 ctgcctgtcc tgccacaaca ttttggggta ttatgatttc cgaagcaggt ttttctttcc
     1681 gcaaacaaag ggaagtaatg ttatgaaatt tgaaaaaata actcgtttct ttcgggatgt
     1741 ccgttccgaa atgaaatgtg taagctggcc taccaagacg gatttgaaag aaggtacatt
     1801 agtagtgatt attatgtctg ctattgtagc catatttttg tccctggttg attttggttt
     1861 taccaagatt gtagaattga ttttctaagg gataggtaca agcagatgaa gtggtatgta
     1921 attcatacct attcaggtca cgaaaataag gttaagacgg ctatagaaaa gggtctggcg
     1981 ggcactcctt tagaaaaaca ggtaggtcgt ttactggttc ctgtgcgcaa gacctttgtg
     2041 attagagaag gtaaaaagat agagcgagat aagaaactct ttacttctta tgtgattatg
     2101 gaagcagata tgacgccgga actaaaaact tatattcttc aaattgcagg agtaaccagt
     2161 tttttaggaa taaataaaga acataaagag cccatacctt tgtctcagga agaagtagac
     2221 cgacttttgg gaataaccga tcccaaccgc gatataaagg tctattcata tatgcctgga
     2281 gatatggtta aagttatttc cggtcctttt accgattttg agggtatcgt acaaaaaaca
     2341 acggaaaacg gaaccaaact aattattgaa gtaactgtgt tcggacgcag aaccccggtt
     2401 gagttaaatg ctagccaggt agaattaatt aaaaaataaa gaagatacaa tagaggtaag
     2461 ataatggcta aacctaaaga tgccgtagca acattaaaac tgcaacttcc agcgggaaaa
     2521 gcaactcccg cacctcctgt gggacctgct ttaggtcagg taggagttaa tattcctgaa
     2581 ttttgccgtc agtttaatga aaaaacagct gatcagccag gaatggtatt ccctgtaata
     2641 atttatgtgg caaagaataa atcttttacc tttgaaatta aaacccctcc agctgcagta
     2701 ctgattaaaa aagaagctgg tttagctacg ggaagtgcca ctcccaataa gcttaaagtt
     2761 ggcaaaatta caaatgagca gttaaaaaac atagctcagc tgaaaataaa agatatgaat
     2821 tgtaattccc ttgaatctgc tatgagtatg atcgctggaa ctgcacgaag tatgggtgtt
     2881 acgatagagg actgatactt cgcttatgca ggagatagta ttcataagga gagtcaatga
     2941 aacatagcaa aaggtataat gttgcctata cgatgtttga ccgccagaaa agatttgatc
     3001 ttaacgaagc aatcaaactt ttgaaaagtt taccaacttc caagtttgac gaaaccgttg
     3061 aactgcattt caatctgggt gtagatcctc gtaaagctga tcaacaaatt cgcaactcac
     3121 ttgttttacc tcatggaaca ggtaaagaga tgcgagtttt agtatttgct gaaggcgaca
     3181 aggcagaaga agccaaaaaa gcaggagccg attatgtggg cgtagatgat ctcattgaaa
     3241 aaatatctaa cggatggttt gattttgatg ttgtgatcgc tactcccaat ttaatgggta
     3301 gaatcggtaa actgggacga gttctcggtc ccagaggatt aatgccaaac cccaaagtgg
     3361 gaacagttac tatggatgtt ggaaaagctg tgcaggaagc aaaaagcgga aaaataacct
     3421 accgcgtaga taaatttgcc aacttgcata ttccggtagg aaaattaagc tttgaggaag
     3481 aaaaactgaa agacaacatc aaagctgttc ttgccgctgt tcttagagac agacccccta
     3541 cacttaaggg tgtttacatt aaaagcatta ccctctgcac gactatgagt cccggaatta
     3601 aaattcaggt cgcaagtgca accgcggaag caagaagtta agggagcagg aaaatggtgc
     3661 aaagtattaa atataagata gtaaaggact tgacagaaag attaagtggc gcaagagcga
     3721 tcgtattggt agattacaag ggaataaata ttgaacaagt gaatcaattg cgcaaccgct
     3781 tcagagaaag tcaagtggat tactttgtcc aaaagaatac cctgattaaa attgccttaa
     3841 atgacttggg tattacggaa ctggaccctt atcttaaggg cccgacagct gtggcagtga
     3901 gtaaattgga tgaggtgtcc cctgcccgcg aactaatcaa attcttaaaa gaggtgatgg
     3961 aagataaagg ttttccttcg tttaaagtcg gttatatagc agggcatatt tttaatgccg
     4021 atgaactgac tgctttggca aaactaccat cccgtgaaga attgcttgct aaagtacttg
     4081 ccggtatgaa tgcaccgctg agtaactttg taagcatcaa ccagggtatc ttgcgcaaat
     4141 ttgtttatgc tcttgatgcc atagctaaaa aacaagctga agccagctaa gaaaaattat
     4201 ttatatatcc aatggaggaa caatgtccga taaaaagcaa caagtaattg atctgattaa
     4261 ggagatgacg gttcttgagc tctctgaatt agtgaaagaa atggaagaaa tatttggtgt
     4321 atccgcttct gctccagtag ccgcttttat gccaacacag ggctctgctg ctgaaggtgg
     4381 agaaaaagag gaacagacag aatttgatgt aattttaacc agtgccggag acaagaaaat
     4441 taatgtaatc aaagtagtgc gagagataac caagcttggt ttgaaagaag ccaaggacct
     4501 tgttgatgga gctcccaaag tggtctccga aaaagttagc aaagaagaag cagaatcaat
     4561 taggaaaaaa ctagaagaag ccggtgcaac tgtcgaagtc aaataaccaa atccttatca
     4621 ggataaaggt ccaaaaaaag tccgcaggga ctttttttgg actaatttct g
//
LOCUS       DAOPFL010000106         4586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_25323_length_4586_cov_132.379828, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000106 DAOPFL010000000
VERSION     DAOPFL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25323_length_4586_cov_132.379828"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..2811
                     /gene="clpB"
                     /locus_tag="PLG69_06660"
     CDS             235..2811
                     /gene="clpB"
                     /locus_tag="PLG69_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPI43225.1"
                     /translation="MNTQRFTIKSQELLADMQDLASSYGHQEIKDLHLLKAMLTQEEG
                     LIQPALEKLEVSIPKVKSEVENALNKLPKVSGSSQQYLSQEVLDILHQAEKEADQFQD
                     DYISTEHLLLAILAKGKEAANILKNAGLQADKLLLALKELRGNQRVTDQNPEAKYQAL
                     EKYARNLTRLARMEKLDPVIGRDEEIRRCIQILSRRRKNNPVLIGEPGVGKTAIVEGL
                     ARRIVARDVPENLLDKELLELDMAAIVAGAKFRGEFEERLKAVLSEVEKSEGRIILFI
                     DELHTVVGAGAAEGSVDASNMLKPALARGNLHCIGATTLTEYRKHIEKDPALERRFQP
                     VLVTEPSVEDTISILRGIREKYEVHHGVQISDNALVAAAQLSERYIADRFLPDKAIDL
                     IDEACALLRMEIDSLPVELDESERRLRQLEIERLSLAKEKDPLSQERLEKIKEEIAQL
                     NEENKALRLRWEKEKQVLEQIRTLKTEIDTLKGEADKAERAGNYEKTAQLRYGTIAAK
                     EKELQKLLQDEAMTKKDRLLKEIVDEEVVAEVVSKWTGIPVSKLAESEMQKLIELENV
                     LAQRVVGQEEGIKALANAIRRSRSGLSDENRPIGSFIFLGPTGVGKTELAKTLASYLF
                     DTEKALIRLDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVLLLDEIEK
                     AHPDVFNVLLQILDDGRLTDGKGRTVDFKHCVIIMTSNIGSQEIYEASDSDLEKIKPR
                     LMNILQQYFRPEFLNRVDDIILFHRLNKDHIKKIVKLQLDILASRLANRNLQLDFSEN
                     LIEHLAEAGYDPAFGARPLKRLIQKEIEDTLAKYILAGQFTSGKKIMLDWDPHQGLII
                     TQ"
     gene            complement(3207..4487)
                     /locus_tag="PLG69_06665"
     CDS             complement(3207..4487)
                     /locus_tag="PLG69_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPI43226.1"
                     /translation="MVQEPQSFHEFMPDKVWKWKILEEKVEKVLSLHDFQEIRLSVLQ
                     DSQVLQDGITALIQGQEADTAMKGVLSLSLPDDQLSLLSLRPEGTISVLHHTARITNP
                     GEIHRFYYSGPMFRRINNQQQMEFYQLGVELLGSESILSENEVISLGMKLCKTLGLNE
                     VRLDLNSYGCFTCRESFFADLNKYLEEHKSEYCNSCYRELSTNPFSQVHCANPDCKKS
                     LEEGPHIYDYLCNSCKTDFNKVKNIQANLGNQYKVNPNLNKNFAYYNKTVFDFIAKHK
                     GKEIIIGGGGRYDYLSALITGKRIPAVGFYLNLDSIFEIMESRGLFEEKDPEFTVYIC
                     AESPNLEMMTLQIVQELHSQQIKTIISPECRPAENEIAIAKKKDCPIMLILRDDNIRE
                     GKILLRNLVKEEQKYISLKDTLPSIMLAKKAMQS"
BASE COUNT         1460 a          840 c          973 g         1313 t
ORIGIN      
        1 agaaaaagtt ccgttaggaa cgacagataa tagcgtcggg ttttgaaccc ggttcaacaa
       61 ttcagagttt tgtgaactct ttggcaaaga aagaaattgt aaattctttt cccaactgca
      121 atattatgtt atgatgtcag ttacaggaaa ttagcaggca gggcagggga ctgctaattt
      181 tttacttgac atataggtaa tgctgattat attttcttgc agttggaggt aattatgaat
      241 acacaaagat ttacaataaa gtcacaagaa ttgcttgctg atatgcagga tttagcttcc
      301 agttatggac atcaggaaat taaagacctg catttgttaa aagcaatgtt aactcaggaa
      361 gaagggctga ttcaacctgc tttggaaaaa cttgaggttt ctattcccaa agtgaaaagc
      421 gaagtggaaa atgcattgaa caaacttcct aaggtaagtg gaagttcaca gcagtattta
      481 tctcaggagg tcttggacat attgcatcag gcagaaaagg aagcagacca gtttcaggac
      541 gattatattt ctactgagca tttgcttttg gcaatcttag ccaagggtaa ggaggcagct
      601 aatatcctga aaaatgctgg tttacaagcc gataaactgc ttttggcgct taaagaattg
      661 cgtggcaatc agcgtgttac cgatcaaaat ccggaagcca aatatcaggc attggaaaag
      721 tatgcgcgca atttaacccg tttagccaga atggaaaagt tggaccctgt tattggcaga
      781 gatgaagaaa ttcggcgttg catccagatt ttatcccgga gaaggaaaaa caatcccgtt
      841 ttaattggcg aaccgggagt tggtaaaacc gctatagtag aaggtttagc ccgcagaatt
      901 gtagctcgtg atgtgcccga gaatcttttg gacaaggaat tactggaact tgatatggca
      961 gcgatagttg ctggtgcaaa attccgaggc gaatttgaag agcgcttaaa agccgtgctt
     1021 tcggaggtgg aaaaatccga aggacgcatt attcttttta ttgatgaatt gcatactgtt
     1081 gttggagcgg gagctgctga gggctcagta gatgcatcca atatgttaaa accggcttta
     1141 gcacggggaa acttgcattg cattggagct acaactttaa ccgaatatag aaaacacatt
     1201 gaaaaagatc cagcattaga acggcgtttt cagccagttt tagtgacgga accctctgtg
     1261 gaagatacaa tttccatttt aaggggtatc agagaaaaat atgaagttca tcatggagtg
     1321 cagataagcg ataatgcttt ggtagctgca gctcaattat ccgagcgtta tattgccgat
     1381 cgttttttac ctgataaagc aattgatttg attgatgaag catgtgcttt gttaagaatg
     1441 gaaattgatt ccttacctgt agagcttgat gaatcggaaa gacgactgcg tcaattggaa
     1501 atagaacgcc tttctttggc aaaggaaaaa gaccctcttt cccaggaacg cctggaaaaa
     1561 atcaaggaag aaattgctca attgaacgaa gaaaacaaag cattacgcct gcgctgggaa
     1621 aaggaaaaac aagtgctgga acaaattcgc actcttaaaa ctgaaattga tacccttaaa
     1681 ggtgaagctg ataaagcaga acgcgcagga aattacgaaa aaaccgctca actgcgttat
     1741 ggaacaatcg ctgctaagga aaaagaactg caaaaattgc tgcaggatga ggcaatgacc
     1801 aaaaaggacc gcctcctgaa agaaattgtg gatgaagaag tagtagccga agttgtttcc
     1861 aaatggacag gtatacctgt ttccaaactg gcggaaagcg aaatgcaaaa actaatagaa
     1921 ctggaaaatg ttcttgctca aagagttgta ggacaggaag aaggaattaa agcattggct
     1981 aatgctatcc gccgcagtcg cagtggatta agcgatgaaa acagacctat cggttccttt
     2041 atctttttag gtcctacggg agttggtaaa acggaactgg caaaaaccct ggcaagttat
     2101 ctttttgaca ctgagaaagc tttgatccga ttggatatga gcgaatttat ggaaaaacat
     2161 agtgtagcac gattaattgg agctcctcct ggctatgtgg gctatgagga aggtggatat
     2221 ttaacggaag cagtccgtcg cagaccttat tccgtgctac ttttggatga aattgaaaaa
     2281 gcccatccgg atgtttttaa tgtcctgctt caaattttgg atgatggacg ccttacagac
     2341 ggaaaaggca gaacggtaga tttcaaacac tgcgtgataa ttatgactag taatatcggt
     2401 tcacaggaaa tttatgaagc gtcggacagt gatctggaaa aaataaaacc acgcttgatg
     2461 aatatcttac agcaatattt ccgtcccgaa ttcttaaacc gtgtggatga tattattctt
     2521 ttccaccgtt tgaataagga tcatataaaa aagattgtga aactgcaatt ggatatttta
     2581 gcatcccgtt tggcaaatcg caatctccag cttgattttt cagaaaatct tattgagcac
     2641 ctggcagaag cgggatacga tccggcattc ggtgccagac cgctcaaacg ccttattcag
     2701 aaagaaattg aggatacttt ggcaaaatat atcctggcag ggcaattcac cagtggaaag
     2761 aaaattatgc tggattggga tccccatcag ggcttaatta taacgcaata acctgcaata
     2821 taagttactt aactaagagg gctggcttac aattggatgc cagccctctt tcttataact
     2881 tgttgtaact ccgcttttta ttctgcaacc caaaatctaa cctgtggaca caaaataagg
     2941 aagttccggt tagttcttcc ttaattatat atggtcataa tacaatggtg tctatcggaa
     3001 atttgccgag agacaccacg acatctctac cacaaataat taagcaagat ataaggcctt
     3061 ttttatatta aaattggact gaagcatcaa gtgcttaaat gacaaacagt gagcatcgta
     3121 agcaataaaa tcagtgtaat ctgcgggaaa caaatttatt attcccaaca ttcggtagat
     3181 tcggttatct gcattctatt aatttgttag ctttgcattg ccttttttgc cagcataatg
     3241 gaaggcagag tatcctttaa agagatatat ttctgttctt ctttcaccag attccgaagc
     3301 aggatttttc cttcgcggat attatcatca cgcagaataa gcattatggg gcagtccttc
     3361 tttttggcaa tagcaatttc attttctgcg ggtctgcatt caggtgagat aatggtttta
     3421 atctgttgtg aatgaagttc ctgaactatt tgcagggtca tcatttccag gtttggactt
     3481 tcagcgcaga tataaacagt aaattcaggg tctttttcct caaataagcc ccggctttcc
     3541 ataatttcaa aaatgctgtc caaattgaga taaaaaccga cagctggaat tcttttgcct
     3601 gtgataagtg ctgaaagata atcgtagcgt ccgccaccgc caataataat ttcttttcct
     3661 ttgtgtttag caataaagtc aaaaacggtt ttattgtaat aggcaaagtt cttgttgaga
     3721 ttaggattaa ccttatattg atttcccagg tttgcctgaa tgtttttcac tttattaaaa
     3781 tctgttttac aggaattaca aaggtaatca taaatgtggg gtccctcttc aagggatttc
     3841 ttgcaatcgg gatttgcaca atgaacttga ctgaaaggat tggtagaaag ctcacgataa
     3901 caggaattgc aatattcgct tttatgctct tccagatatt tattcaggtc tgcaaaaaat
     3961 gactcccgac aggtaaaaca gccataagaa tttagatcaa ggcgaacttc attcaaaccc
     4021 agtgtcttac aaagcttcat tcctaaagaa attacttcgt tttcggaaag aatgctttcg
     4081 ctgcctaaaa gttccactcc caactgataa aattccatct gttgctgatt gttaattcgg
     4141 cgaaacatag gacccgaata gtaaaaacgg tgaatttcac ccggattggt aatacgcgct
     4201 gtatgatgta aaacactgat tgtgccttca ggacgcaaac tcaataaact taattgatca
     4261 tcaggaaggg aaagagaaag aactcctttc attgcagtat ccgcttcctg accttgaatt
     4321 aaagcggtta tgccatcttg taagacctga ctatcctgta aaacagaaag acgaatctcc
     4381 tgaaagtcgt gcaatgataa aactttttcc actttttctt ccaggatttt ccatttccag
     4441 accttatctg gcataaattc gtgaaaactt tgtggttcct gaaccatttt ttctcctcaa
     4501 aagtccttca ctattaaaga taaatcaaat agaccaactt ggcaataaga ttttagaacc
     4561 tctcacaaat tacacagata gatttt
//
LOCUS       DAOPFL010000107         4578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_25389_length_4578_cov_115.360822, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000107 DAOPFL010000000
VERSION     DAOPFL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25389_length_4578_cov_115.360822"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1109
                     /gene="rfbB"
                     /locus_tag="PLG69_06670"
     CDS             39..1109
                     /gene="rfbB"
                     /locus_tag="PLG69_06670"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPI43227.1"
                     /translation="MNRVIVTGGAGFIGANYLHTLFADPSFTGKILNIDKLTYAGNLE
                     SLQDLEAKYGNSRYFFEQADICDAPKMKEIMHQFQPDTIVHFAAESHVDRSIDGPMEF
                     VQTNLVGTATLLNSALEYYRTLDEEAKKRFRFHNVSTDEVFGSLGENGMFTEETPYDP
                     SSPYSASKAGSDHLVRAWQRTFGLPITISNCSNNYGPYQFPEKLIPLMILNCLAHKPL
                     PVYGKGINVRDWLFVTDHCEAINIIIRKGKIGETYNIGGHNEMKNIDIVTTICKILDE
                     MEPSKKLKSYTELITFVQDRPGHDLRYAIDATKIEKELGWKPAETFATGIRKTVAWYL
                     ENKQWWQNIQNNKYRQERLGIG"
     gene            1345..2469
                     /locus_tag="PLG69_06675"
     CDS             1345..2469
                     /locus_tag="PLG69_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPI43228.1"
                     /translation="MKNDVVSYFLELVSIDSESGNERAIVDKLKKDLEELGAIVEEDD
                     CASKIEGNAGNLYAYFPGTINKKPILLCAHCDTVVPGKDIQPIMENGRIFTDGTTILG
                     GDDKSGIAEILMAIKQIKEKGIEHPPIEVLITVSEEIGLLGARNFDISKLQAGFGYAL
                     DTHQVGEVVIGAPSQISFQINFYGKEAHAGVEPEKGLNAIRLAAEAIALMPNGRIDFE
                     TTCNLGIIKGGTATNIVPGLVTVEGEVRSHNKGKLQKVCEEIEQAVISTVHKFNANGA
                     KASFHFELKEEYEAFLIDRNAPVVTLAEKALQNLHLPVDIKVGGGGSDANIFNANGLP
                     MIIVGTGMNNVHTTEEDILVDELYRGTAFVEELIHLYAEE"
     gene            2470..3219
                     /gene="dapB"
                     /locus_tag="PLG69_06680"
     CDS             2470..3219
                     /gene="dapB"
                     /locus_tag="PLG69_06680"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPI43229.1"
                     /translation="MAVKICLLGYGKMGKMIAELASDFDCTVVSKIDPNQKDCYQEIS
                     EQSLQDADVCIDFSHPSVVLENIHKVLALKKNMVVGTTGWFEHLKEIQQQVQQAGTGF
                     LYGANFSIGMNIFKRLVAEATKAFDRFPEFDVYGWEMHHNQKADSPSGTAIELSKTIL
                     ANSSRKKTALFDPAYRKIEPEELHFGSLRAGSFPGTHIIGFDSATETIELSHCVRSRS
                     TFAYGALQAAKWIKDRKGFYSFAEMVEEVLC"
     gene            3213..4001
                     /gene="dapF"
                     /locus_tag="PLG69_06685"
     CDS             3213..4001
                     /gene="dapF"
                     /locus_tag="PLG69_06685"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPI43230.1"
                     /translation="MLIPFRKMHAQGNDFVILDFFAPESFVLAKLDFSSVAEELCKPH
                     FGIGADGLVLLMPNESCDAQMFIYNADGSTAEMCGSALRCVTWILEQKTGKKEFRVQT
                     EAGLKTCYVNEDNTITVNLGMPSMLKSAYQVMDFTGDLVEIGNQHFIVWVNSLQDDPH
                     LQYGSAIEHHKGFAQTVNVHFAVLLNRTAVAIKIWEKGCGATLACGTGASATVFSGIA
                     KGLLDNEVEVLMPGGIVKINASESGYLLSGEVCESFQGVYNWKI"
     gene            3989..>4578
                     /locus_tag="PLG69_06690"
     CDS             3989..>4578
                     /locus_tag="PLG69_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPI43231.1"
                     /translation="MENLGKYFLELRLQREYSYQDIWNELRIPENQIKAIEENRFTEL
                     GPYGVAKTLVYNYARFLEADVDSVMNAFSSLMPESAKPEFQPATPVKENKIMLSTNFM
                     WLIGILLIIVILGSILWYAYNQNWLKMPDFFASKKPDSTAVIIKEEKEETKPDTLRQR
                     LRAISESIPQTNSVSTPKSETKTVPPDTTDYIGNILG"
BASE COUNT         1396 a          845 c         1006 g         1331 t
ORIGIN      
        1 gcatttagaa caatcctaaa taaaataagg caggaaaaat gaacagagtt attgttaccg
       61 gtggagccgg ttttatcggt gccaactatt tacatactct ttttgcagac ccttctttta
      121 ctggtaagat attgaacata gataaactta cctacgcagg taatttggaa agcttgcagg
      181 atttggaagc caagtatggt aattcgcgtt atttctttga gcaggcagat atctgcgatg
      241 caccaaaaat gaaagaaatt atgcatcagt ttcaaccgga tacaattgtc cattttgctg
      301 ccgaatccca tgtggataga agtattgacg gtcctatgga atttgtccaa accaatttag
      361 taggaacggc aactttgctg aattccgctc tggaatacta tcgcactttg gatgaagaag
      421 caaaaaagcg ctttcgcttt cataatgtat ctacggatga ggtttttggt tctttaggtg
      481 aaaacggaat gtttaccgag gaaacacctt acgatccttc ttcaccctat tctgcatcaa
      541 aagctggttc agaccattta gtgcgtgcct ggcaaagaac cttcggactg ccaatcacca
      601 tctccaattg ctccaataac tacggacctt atcaatttcc tgaaaagctg attcctttaa
      661 tgattttgaa ctgcctcgct cataaacctt tgcctgttta cggaaaagga attaatgtgc
      721 gggactggct ttttgttaca gaccattgtg aagccatcaa tattattatt cggaaaggaa
      781 aaataggcga gacatataat attggcggtc ataatgaaat gaagaatatt gatattgtta
      841 ccactatctg caagattttg gatgagatgg agccctcaaa gaaactgaaa agctatacgg
      901 aactgataac ttttgtgcaa gatagaccag gtcacgattt acgctatgcc attgatgcca
      961 ctaaaattga aaaagaactt ggttggaaac ctgccgaaac atttgccacg ggaattagaa
     1021 aaacagttgc ctggtatctg gaaaacaaac agtggtggca aaatattcaa aataataaat
     1081 accgtcagga acgcttggga attggataaa acaggatgaa agaactccct gtagttttct
     1141 ataagttgac caggaggtaa ccctttcgga aaatcagcag agccatatat tggcaaccca
     1201 gatttttgcc gatttttctt tctacttggt gttctcaatg atttcggtgg ttttggtgta
     1261 aaaaatccat agaataaata gttgctatat accgaaattt tggctattat atatttagta
     1321 aggaatgcat aaaaggaagt cccaatgaaa aacgatgtag taagctactt tcttgagctc
     1381 gtctctattg atagcgaatc tggtaacgaa agagccattg tggataagct aaaaaaggat
     1441 ttggaagaac ttggtgctat tgtagaagaa gatgactgcg cctcaaaaat tgagggcaac
     1501 gcgggtaatc tctatgccta ttttcccgga actatcaata aaaagccaat ccttctttgt
     1561 gctcattgtg atactgtagt tcccggcaaa gacattcagc caattatgga aaacggtcgc
     1621 atttttacgg atggcacaac tattttaggc ggagatgata aatccggcat tgcggaaatc
     1681 cttatggcta ttaaacaaat taaggaaaag ggcattgagc atcctcctat tgaggtgttg
     1741 ataactgtct ccgaagaaat agggcttttg ggtgccagga attttgatat ttccaagctg
     1801 caagccggtt ttggttatgc attggatact catcaggttg gggaagtggt tattggagcg
     1861 ccttctcaaa tttcttttca gattaacttt tatggcaagg aagcgcatgc gggtgtagaa
     1921 cctgaaaaag gattaaatgc cattcgcctg gctgcagaag caattgcact tatgcctaat
     1981 ggcagaattg attttgaaac tacttgtaat ctcggtatca ttaaaggtgg aacagctact
     2041 aatattgttc ccgggttagt aactgttgaa ggtgaagttc gcagtcataa taagggaaaa
     2101 ctgcaaaagg tctgtgagga gattgaacag gcagttattt ctacggtcca taaatttaat
     2161 gcgaatggag ccaaagcatc ctttcatttt gaactgaaag aggaatacga ggcattttta
     2221 attgatcgta atgctccggt agtaaccctt gcagaaaagg cattgcagaa tttacatctt
     2281 ccggtagaca tcaaagttgg aggtggtgga agtgatgcta atatttttaa tgccaacggc
     2341 ttgccaatga ttattgtcgg caccggaatg aataatgtgc atactacgga agaagatatt
     2401 ctggtggatg aactctatag aggaactgct tttgtagaag aactcatcca tctttatgcg
     2461 gaggaataaa tggctgttaa aatctgtttg ttaggttatg gcaaaatggg aaaaatgatt
     2521 gccgaattgg cttctgattt tgattgcacc gttgtttcta agatagaccc taaccaaaag
     2581 gattgctacc aggaaataag tgagcagtcc ctgcaagatg cagatgtttg cattgatttc
     2641 agccatccct ctgtagttct ggagaatatt cacaaagttc ttgctctcaa gaaaaatatg
     2701 gtagttggca caaccggctg gtttgaacac ctaaaagaaa tacaacagca ggttcagcaa
     2761 gcaggaacag gttttcttta cggagccaat ttttctatcg gaatgaatat ctttaagcga
     2821 cttgtagcag aagcaactaa ggcatttgac cgttttccgg aatttgatgt ttacggctgg
     2881 gaaatgcacc ataatcaaaa agcggatagt ccttcgggaa ctgccattga actttctaaa
     2941 accattttag ccaatagcag caggaaaaaa accgctcttt ttgaccctgc ttaccgtaaa
     3001 atagaacctg aagaattaca ttttggctca cttcgcgccg gttcttttcc cggaacgcat
     3061 ataattggtt ttgatagtgc aaccgaaacc atagagctta gccattgtgt ccgttcgcgt
     3121 agcacttttg cctacggagc gttacaggca gcaaaatgga ttaaagaccg caagggattt
     3181 tacagttttg cggaaatggt agaggaagta ttgtgctgat tccttttcgc aaaatgcacg
     3241 ctcaaggtaa cgattttgtt atcttggatt tctttgctcc ggagtcattt gttctggcaa
     3301 agctggattt ttcttctgta gcggaagaac tttgtaaacc acattttggc attggagcgg
     3361 atggactggt tttacttatg cctaatgaat cctgcgatgc gcaaatgttt atttacaatg
     3421 cagatggctc aacagctgaa atgtgcggtt ctgcattacg ctgtgtaact tggattttgg
     3481 agcaaaaaac cggaaaaaag gaatttagag tccaaaccga agcgggactt aaaacctgtt
     3541 atgtaaatga ggataacact ataaccgtta acctgggaat gccttcaatg ctgaaaagtg
     3601 cctatcaggt aatggatttt accggcgact tggtggaaat tggcaatcag cattttattg
     3661 tttgggttaa ttctttgcaa gacgatcccc atcttcaata cggttctgcc atagaacatc
     3721 acaagggatt tgcacaaacg gtaaatgtgc attttgccgt tttactgaat agaacagcag
     3781 tagccatcaa aatctgggaa aaaggttgcg gagctactct ggcttgtgga acaggagctt
     3841 cagctacagt ttttagtggc attgccaagg gcttattaga taatgaagtt gaggtcttaa
     3901 tgcccggagg aatagttaaa atcaatgcct ccgaaagtgg ctatttatta agcggagagg
     3961 tctgcgagtc atttcaggga gtttacaatt ggaaaattta ggcaagtatt ttctggaatt
     4021 gcgtttacag cgtgaataca gctatcagga tatctggaat gaactgcgta ttccggaaaa
     4081 tcagataaag gcaattgaag aaaatcgctt taccgaactt ggtccctatg gagttgctaa
     4141 aacattggtc tataactatg cccgttttct ggaagcggat gtggattccg taatgaatgc
     4201 tttttccagt ttaatgccgg aaagtgccaa accggaattc caacccgcaa ctcctgtgaa
     4261 agaaaacaag attatgcttt ccaccaactt tatgtggctg attggcattc tattgattat
     4321 tgttattctc ggctccattc tttggtatgc ctacaatcaa aattggctga aaatgcccga
     4381 tttttttgcc tctaaaaaac ccgattccac tgccgttata attaaagaag aaaaagaaga
     4441 aaccaaacca gataccttgc gtcaacgctt gcgtgctatc agtgaatcca tccctcaaac
     4501 taatagtgta tctactccca aatccgaaac aaaaactgtt ccaccggata caactgacta
     4561 cattggcaat attttagg
//
LOCUS       DAOPFL010000108         4493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_26097_length_4493_cov_103.422037, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000108 DAOPFL010000000
VERSION     DAOPFL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26097_length_4493_cov_103.422037"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3133)
                     /locus_tag="PLG69_06695"
     CDS             complement(<1..3133)
                     /locus_tag="PLG69_06695"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI43232.1"
                     /translation="MAENITQQLDAYITNKIALSKAMGYLSDSAKSHLIEIVGKSGSG
                     KSHFVQPLMESVEDIYAKRIYFSPHPLMLNHFSELMQVITEVTEEDLYGYYREFRENI
                     GSVNKYDFFFYITERLNAMELFQPFLLVIDDCDILDNYTRDYLQYLVQYLPDTGIQIV
                     VFTQTRLFPFAELDNIPTLGVEECQRLLQLTFPETDFTGSSESEIFQKITSGNLMIIE
                     KVLKEMKSQKPKGKFNLGPYLEKTFDAEQIYYETLETLSSSQKEILTAMYVLDGFDAS
                     ALAKALGNPKNIKTDLVTLYNLNLISKPEKGLSIQKKKAFSEWLNNNLKEFKGTLLNQ
                     LMEYLQKEKKHPQILVSLQMLNKKYNSALFGKVISELSQINDSASVLKLEEYVLAHTK
                     SPADKLASTQRIAEAYSVLNQKDKAIDHFRQALHISTENNIPAEQIVYKLASDLYAVN
                     SSAFALEIIKQYSPATIDPYWKVKILLLKADIQTESEAFNEAFNTLDIASHSSNSITN
                     QQQRHSVQAEAKKIRGKIHYYINEWEQAEEVFNEAETLYSLAKDHSGLAAIYNNLGVL
                     YMCQGFWEKSETYFLKSLALEKQYFNLNGISVCYNNLGGLMDDKGDPAKSLYYLEEAL
                     KIQKLLAEPYNITNIYNNIGVTLMDHGDYDRAEDALKKSLDMANEFGFQRNTIATLNN
                     LGGLYFKKGDWAESISYYEKAIKLSQESGFNEGLLRSFNNLGEVYEKQDELNLAYDLY
                     IKGLELLPAVSDDYIKAELYGNLGSVFTKLHKFKDAYRYLVESLDFFKNIAARDKIIE
                     GYLKQAYYFILTRNIESADYYLAEAQKLAIEQNLPFEIGRSLYLRALLERKNLESAKN
                     YLNEAIKHFVEAGSNYELSLANYELAGVLLDMEEWEQAMEILKNNRKIIERYGSIKLL
                     EQNDILMQKISREYSSQMEEVKFEENLLNQFYEITQNLNTITDLDVLIEQSLSSLVEI
                     ANADGGMLLLHPNPSLPDAWEYRIYHNFSPEDKYHDQFQNICYEVYQNNKLENIKQPH
                     FAPSYNNILALPLS"
     gene            complement(3472..>4493)
                     /locus_tag="PLG69_06700"
     CDS             complement(3472..>4493)
                     /locus_tag="PLG69_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43233.1"
                     /translation="FKKFELPYCFRCTKVIIGAVKDIIIQAKAEGHLHNRIEKRYDCY
                     IDKKGEDSQRNPHIITAQLKLASSLPKFILREISNIPVTDVRESWDDSYPTVLIVGSS
                     HYLELIYKEIHALPNVDWKKTKEDKPIISDAYEYLLKDFNSNLGWRILMELELQKGIA
                     RNVLINCPDGLLSKNLPKDFVKKHLEITKLIQKIDADYVDPSILHQLRQYLREKDYVC
                     VVDRFVNSIAPLSEDKTKLSIKLSTYMGCKGLSAGYVFIVGFNNKVIPKNKHLISDVE
                     ICKFIVALTRTRKKCYILTDKYFSTPKRKDGTWIQAKEKSDLLKWIDSSRLLDMGVIS
                     ASDMH"
BASE COUNT         1350 a          905 c          738 g         1500 t
ORIGIN      
        1 tggaaagagg cagagctaaa atattattat aactgggagc aaaatgcggc tgtttgatgt
       61 tttccagctt attattctga tacacttcat aacaaatatt ctgaaattgg tcgtgatact
      121 tatcttcagg agaaaaatta tgataaattc tatattccca ggcatccggc aaactcggat
      181 tgggatgcag taaaagcatt ccgccatcgg cattggcaat ttccaccaaa ctggaaagag
      241 attgttcaat cagcacatcc aaatcggtta tcgtattaag gttttgggta atttcataga
      301 actgattcag caagttttct tcaaatttta cttcttccat ttgcgaagaa tattcacggg
      361 atattttttg catcaggatg tcattttgtt ccagtaattt aatagagccg tatctttcaa
      421 ttattttgcg gttatttttc aaaatttcca ttgcctgttc ccattcttcc atatccaaaa
      481 gcacaccggc aagttcataa ttggctaaag aaagttcata gttactacct gcttctacaa
      541 aatgtttaat tgcctcattc agataattct ttgcggattc caaatttttc ctttccagca
      601 aagcacgcag gtataaagaa cgccctattt caaaaggcag attttgttca atagccagtt
      661 tttgagcttc cgctagataa taatcggcac tttcaatatt gcgggttagg ataaagtaat
      721 atgcctgttt cagataaccc tcaataattt tatcccgggc agcaatgttt ttaaagaaat
      781 ccaagctttc aaccagataa cgataagcat ccttaaattt atgcagctta gtaaaaacac
      841 ttcccagatt gccatacaat tctgccttaa tataatcatc cgaaacggca ggaagcaatt
      901 ccagaccttt aatataaaga tcataagcca ggttcagttc atcctgtttt tcataaacct
      961 cgcccaagtt attaaaactg cgcaatagac cttcgttaaa accgctttcc tgagagagtt
     1021 taatggcttt ttcatagtaa gaaatggatt ctgcccagtc acctttcttg aaatataaac
     1081 cgcccaaatt atttaaagtg gcaatcgtat tacgctgaaa accaaattca ttagccatat
     1141 ccagcgactt ttttaaggca tcttcagccc tgtcatagtc accgtggtcc attagcgtaa
     1201 cacctatgtt attatagata ttagtaatat tatagggttc agccaaaagt ttctgtattt
     1261 taagtgcctc ttccaggtaa taaagtgatt ttgccggatc acctttatca tccatcaaac
     1321 cgcctaagtt attataacat accgaaatgc cattcagatt aaaatattgt ttttccagtg
     1381 ccaaactttt cagaaaatag gtttcgctct tttcccaaaa accctgacac atatacaaaa
     1441 cacccaaatt gttatagata gcagctaaac cgctgtgatc tttggcaaga gaataaagcg
     1501 tttctgcttc gttgaaaacc tcttccgcct gttcccattc atttatgtaa tagtgtattt
     1561 tcccgcgtat ttttttggct tcggcttgaa cgctgtgtcg ttgttgctga ttagttatgc
     1621 tgtttgaaga atggctggca atatccaatg tgttgaatgc ttcattaaat gcttcacttt
     1681 ccgtttgaat atcagctttt aacaggagaa tttttacttt ccaatagggg tctatcgtag
     1741 ccggagaata ctgtttgata atttccaaag caaaagcgga agaatttacg gcatagagat
     1801 cactggcaag tttataaacg atttgctctg caggaatatt gttttccgta gaaatatgca
     1861 atgcctgacg aaaatgatct atcgctttgt ctttctgatt tagcactgaa tatgcttcag
     1921 caattctttg cgtgctggct aatttgtccg cgggactttt agtatgcgcc aaaacatatt
     1981 cttccagttt taacacactt gcggaatcgt tgatctgaga tagctcggaa attactttac
     2041 caaagagagc ggaattgtat tttttattta acatctgcag agaaaccaaa atctggggat
     2101 gctttttctc cttttgcaga tattccatca gctgattcaa taaagtgcct ttgaactcct
     2161 tcaaattgtt attcagccat tccgaaaatg ccttcttctt ctgaatggac aatccctttt
     2221 ccggtttgga aatcaaattc aaattatata aagtaaccaa atccgtcttt atatttttgg
     2281 gattgcccaa cgctttagct aaggcactgg catcaaatcc atccaataca tacatcgcag
     2341 ttagaatttc tttctgtgag gaactaaggg tttccagcgt ttcataataa atctgttcag
     2401 catcaaaggt cttttccaga taaggtccca aattaaactt cccctttggc ttttggctct
     2461 tcatctcctt caagaccttt tctatgatca tcaggttgcc ggaagttatt ttctgaaata
     2521 tttcgctttc actgctacct gtaaaatccg tctctggaaa ggttaattgt aaaagccgct
     2581 gacattcctc cactcctaat gtgggtatgt tgtccaattc cgcaaaagga aataaacgcg
     2641 tttgcgtaaa aactactatc tgaattcccg tatctggtaa atattgaacc agatattgta
     2701 aataatcacg ggtataatta tctaaaatat cacaatcatc aatcaccagc agaaatggct
     2761 gaaacagttc catcgcgttt aaacgctccg taatataaaa gaaaaaatcg tatttattta
     2821 cggaacctat attttcacga aattcccgat aataaccata caggtcttct tccgttacct
     2881 ctgtaatcac ctgcataagc tctgaaaaat gatttaacat aaggggatga ggggaaaaat
     2941 atatccgctt cgcatatata tcttccacac tttccattaa gggctgaaca aaatgacttt
     3001 taccagaacc cgatttgcct actatttcaa ttagatgact ctttgcactg tcacttaagt
     3061 aacccattgc cttggaaagc gctatcttgt ttgttatata tgcatccagc tgttgggtta
     3121 tgttctctgc catttttttc acccctcttg gaatttcgtt tacagtgcac ctgtaaatag
     3181 tgtttacaac ctgtcaagta aaaattataa tttagatgtc ctcccccctt tttttagacc
     3241 gtaggacgat actttcatag cgatggtagc gtaagtccct cgctaaaaat ggaaaaatca
     3301 ctaagaagtg acacctgctt gaaaaaacta cagaaaagca ctcagtttgt gccaataatt
     3361 tgttaaatca tcggagtcaa gagccaatca aatgtgacag ctatttgtca aaccatgaca
     3421 gcaaaatggc aggctatgac acctatttgt catacaattc caaataatta tctaatgcat
     3481 atctgaagct gaaataactc ccatatccaa caatcttgaa ctatcaatcc atttaagcaa
     3541 atcgcttttc tcttttgctt gaatccaagt accatctttt ctttttggcg tagagaagta
     3601 cttatctgtc aatatatagc atttcttgcg ggttctagtt aaggcaacga taaacttaca
     3661 tatctctaca tcggatatga ggtgtttgtt tttaggaatg accttattgt taaaacctac
     3721 gatgaacaca tatcctgctg acaacccttt acatcccatg tatgtcgaca atttaattga
     3781 aagctttgtt ttgtcttcag agagaggagc aattgagtta acaaatctgt caactacgca
     3841 tacataatcc ttttctcgaa gatattgcct aagctgatga agaatacttg gatctacata
     3901 atctgcatca attttttgaa taagtttagt aatttctaaa tgcttcttaa caaaatcttt
     3961 gggtaagttc ttcgataaca atccatcagg gcaatttatt aatacatttc gtgcaattcc
     4021 tttttgtaat tcaagctcca taagaattcg ccaaccgagg ttggagttaa aatctttgag
     4081 aagatactca tatgcatcgc tgattattgg tttatcttct tttgtttttt tccagtcaac
     4141 gttgggaaga gcgtgaatct ctttgtatat caattctaaa tagtgactac ttcccacgat
     4201 caatacagtt ggatacgaat catcccagga ttcccgtaca tcagttactg gaatgtttga
     4261 tatctctcga agaatgaact taggtaatga agatgcaagt tttaattgtg cagtaattat
     4321 atgagggttt ctttgactat cttccccttt cttgtcaata tagcaatcat atctcttttc
     4381 tattctatta tgtaaatgac cctcagcttt agcttgaatg atgatatcct taacagcccc
     4441 aataatcact tttgtacacc tgaagcaata tggtaattca aattttttaa agt
//
LOCUS       DAOPFL010000109         4386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_27017_length_4386_cov_67.391134, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000109 DAOPFL010000000
VERSION     DAOPFL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27017_length_4386_cov_67.391134"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..3181)
                     /locus_tag="PLG69_06705"
     CDS             complement(47..3181)
                     /locus_tag="PLG69_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43234.1"
                     /translation="MKKNTILIFFVLVAALLSAQKMFLQKTQQFHKLAEHPYQNQLIQ
                     YNFRQDLLKKDKTNFNRLLVILVDFAEEYPDDPNTTGNGKFQLEPDSTYIYSVGAPPH
                     NREYFEANLEALKYYYLAVSAGAYELHYDVYPKNMPAYTLPKSMGYYNPPGASSELFV
                     SRMEEYFKTAFETADSIDEEIDFASYGHYMIIHSGSDWQHDIMGDTPSDLPSFFIKVG
                     DGKEAVVDNGTVLISHSCNVPETISQDFYENEENGTIIHSGFGALNAVIAHEFGHSLG
                     MVDLYNVYNSSPMVGVFDIMDSGGSGILIDELDNGDLVYVEGALPTLPGAFSRNLMFR
                     DSYLERGLLKEFPDFNTMTELSLSAISAMQNGNDLVPHTYKIPLNSKEYILVENRNVD
                     PDGDGGTAVFGALDGRVILYPTPFEDISPPPPTYEYDYLLPSFMKANGSAIGGGILVW
                     HINEAILYDEGQTDSSGNWTSNFDANTVNTGFYHRGVSVIEADGLTDLGSNYSMYWTG
                     TPYEYFHKYKPILDTNGLFVNWSQEPWKPELTAVTKPPLVDSFNVPGLYHLKEIGNPA
                     KKMSFKVESGSFDTTQIFDYATSSFIIGPVIKTGFNEDNIPVLIDNKMFLLSYWDNEW
                     QDIIGEFVTNFPRSDYPLQKVDLNTDGYVELVWSKQNTLVLVDWSLDTPIFHQISFPD
                     SITTTPLPVNNTLIVTTKDCIYTVKNFAIQNFISLQGSKCIAGYDDKIIVLGTDFLAA
                     LTLDNLDMFSEFTLPEPCGNIEPLIYTNPDKTIEMIFVMANSGNLYRIYQNRLELIFA
                     NHSKEKPGQLACSSLGDISPVLFFGIGNKQYALKADGTKLTHFPVIAPKAISPCETPF
                     ALKNGSAEILFYPLAENGYLAIDKMGRIVPEMCLSVNKDKKNDYLYYQPEQQILNWYY
                     PDSTGKLFIHSKKNIDTNPILFAGYRNGASGCATFAFRDENISSTEKIAYIYPNPVRK
                     PYYKLNLQNYFGETKLHLYDISGTLVKKMIIPESNNNPRDFELNTLGLSSGVYIMVLE
                     NNGSTKRLKFAVEK"
     gene            complement(3410..>4386)
                     /locus_tag="PLG69_06710"
     CDS             complement(3410..>4386)
                     /locus_tag="PLG69_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPI43235.1"
                     /translation="PFALSGISQATMLFLTFEPGARANAMGRAYSAIADDAYAMWWNP
                     GATAFNRHTQIAATHIPWLQGSGVDDIFYEYLGFNNYFYGIGNINAHIIWLDEGTQPQ
                     TNAQNVELGDFHTYEVAGALGYGYEVVPEKIGLGGNFKLLYSYLGPGTGLTETEGSAF
                     SFAFDVGALFKDVVVDNLNASVVLQNIGPDITYIDEEQSDPAPLTFRAGLGYKIFDNP
                     MGRLIATAEMSKILANEDPLYERFITGWKYFDETIYGVGAEYTYLNLISLRGGYFSDK
                     VGEVEGASFGVGVQYKFSKRYKLGFDFAMVPAGGMTDYNKIFSLGFEF"
BASE COUNT         1421 a          874 c          752 g         1339 t
ORIGIN      
        1 taaaaattag caggataaat ttcttcattg ctaaccccct aaaaaatcat ttctctactg
       61 caaatttgag gcgtttcgtg gaaccattat tttccagaac cataatataa accccggaac
      121 taagtcccaa agtgtttaat tcaaaatccc tcgggttatt attggattcg ggaataatca
      181 tcttttttac caaagttccg ctaatatcat aaagatggag ttttgtttct ccgaaataat
      241 tttgcaaatt aagtttataa tatggtttcc gcacaggatt gggataaata taggcaattt
      301 tttccgttga actgatattt tcatctctga aggcaaaagt ggcacatccg cttgctccat
      361 ttctgtaacc ggcaaaaaga atagggttag tgtcaatatt ctttttactg tgaataaata
      421 gctttcctgt gctatcagga taataccaat tcaggatttg ctgttccggc tggtaatata
      481 aataatcgtt ctttttatct ttatttacgg ataaacacat ttccggaact attctaccca
      541 ttttatctat agctaaataa ccattttccg ctaaaggata gaagagaatt tcggcagaac
      601 cgttttttag tgcaaaggga gtttcgcagg gagaaattgc tttcggagca ataaccggaa
      661 aatgagttaa ttttgtgcca tccgctttta aggcatattg tttgttaccg ataccaaaaa
      721 acagaacggg ggaaatatcc cctaaagagg aacaggcaag ttgaccaggt ttttccttac
      781 tatgattggc aaagattaat tccagacggt tttgataaat ccgatacaaa ttgccgctat
      841 ttgccattac aaaaatcatc tcaatggtct tatcgggatt agtgtaaata agcggttcaa
      901 tattgccaca aggttcagga agtgtaaatt cactgaacat atccagattg tctaatgtta
      961 gagcagccaa aaagtccgtt cctaaaacta taattttatc atcataaccg gcaatacact
     1021 tggaaccttg taaactgata aaattctgga tagcgaagtt tttcactgta taaatacaat
     1081 cttttgtggt tactattaat gtattattta caggtaaagg ggtagttgta atagaatcag
     1141 gaaaggaaat ctggtgaaaa ataggcgtat ctaaagacca gtccactaaa accaaggtat
     1201 tttgttttga ccaaaccaat tcaacatagc catcagtatt gaggtctacc ttttgcagag
     1261 gataatcaga acgcggaaaa tttgtaacaa actcgcctat aatatcctgc cattcattat
     1321 cccaataact gagcaggaac attttgttat caataagtac aggaatgttg tcttcattga
     1381 atcctgtttt tataactgga ccgataataa aggaactggt agcataatca aaaatctgag
     1441 tggtatcaaa actaccgctt tctaccttga aactcatttt ttttgccgga ttaccgattt
     1501 ctttcagatg atataaaccg ggaacattga aactatctac caaaggtggt ttggtgacag
     1561 ctgttaattc cggtttccaa ggttcctgac tccagtttac aaataagccg ttggtatcca
     1621 aaatgggttt atacttatga aaatattcgt acggagttcc tgtccaatac atagaatagt
     1681 tactacccaa atccgtaagt ccatctgctt caattacgga aacacctctg tggtaaaaac
     1741 cggtattaac ggtattggca tcaaaattac ttgtccagtt gccactgcta tccgtttgcc
     1801 cttcatcata taaaatagct tcattgatat gccagacaag tataccgcct ccaattgctg
     1861 aaccatttgc tttcataaaa gaaggtaaaa gatagtcata ttcgtaagtt ggaggtgggg
     1921 gtgaaatatc ttcaaaagga gtaggataga gaataactcg tccatccaaa gctccaaaaa
     1981 cagctgttcc tccatcgcca tcagggtcta cattgcggtt ttccactaaa atatactcct
     2041 tgctatttag gggaattttg taagtatgag gaacaagatc atttccattt tgcatagcac
     2101 tgatagcaga taaggataat tctgtcattg tgttaaaatc agggaattct tttaataacc
     2161 cgcgttctag ataactatcc cgaaacatca aattgcggga aaaagctccc ggtaaagttg
     2221 gtaaagcacc ttccacataa accaaatctc cattatccag ttcatctatt aaaatgcctg
     2281 aaccgccact atccataata tcaaacacac caaccatcgg agaagaatta taaacattat
     2341 acaaatccac cattcctaaa gaatgaccga attcatgagc tattactgca tttaaggcac
     2401 cgaagccact gtgaattata gtaccgtttt cttcattttc ataaaaatcc tgggaaatgg
     2461 tttcgggaac attacaggaa tgcgaaatca ggactgttcc attatctact actgcttctt
     2521 taccatcacc aaccttgata aaaaaggatg gcaaatctga tggtgtatct cccataatat
     2581 catgttgcca atccgacccg gaatgaataa tcatataatg tccatagctg gcaaaatcaa
     2641 tttcttcatc tatgctatca gctgtctcaa aagcggtttt gaaatattct tccattcggc
     2701 tgacaaataa ttcggaactg gctcccggag gattatagta acccatactt tttggcaaag
     2761 tataggcagg catatttttg ggatagacat cataatgaag ctcataagca ccggcagaaa
     2821 ctgctaaata ataatatttc aatgcttcca gatttgcctc aaaatattct ctgttatgtg
     2881 gaggtgcacc aacagaataa atataagttg aatcaggttc caattgaaat tttccattac
     2941 ccgtagtatt gggatcatct ggatattctt ctgcaaaatc caccaggata accaataacc
     3001 tgttaaaatt tgttttatcc tttttaagca agtcctgcct aaaattatac tgaattaatt
     3061 gattttgata gggatgctct gccagcttat gaaactgctg tgttttttgc aaaaacattt
     3121 tttgtgctga taacaatgct gctaccagca caaaaaagat caggattgta ttctttttca
     3181 taattttatc gggaaagttt aagtccgctt caactgtatg ttgttagtcg tttgcacctt
     3241 cattatttta ttcccaagaa atttcttcac aggtgcaaag gactcataac gattttatga
     3301 tcatattacc gtaaaaatta gcggtaacga agtaattttg tagaatttga atgatgaggg
     3361 catcgttctt ccggtgtctt tgaacgaagg aaatatcgtt cttcctatgt tagaactcaa
     3421 agcccagaga gaagatttta ttataatcag tcataccccc tgcaggaacc attgcaaaat
     3481 caaatcccag tttataccgt ttactgaatt tgtattggac acctacacca aaactggcac
     3541 cttcaacctc accaacttta tcagagaaat atcctcccct caaagaaatc agattcaggt
     3601 aagtatattc tgctccaact ccataaattg tttcatcaaa atacttccag ccagtaataa
     3661 atctttcgta gagaggatcc tcatttgcta atattttact catttcagca gttgcaatta
     3721 aacgtcccat tgggttatca aaaattttat aacccagccc ggctctgaaa gttaaaggag
     3781 ccggatcaga ttgctcttca tcaatatatg taatatccgg acctatattt tgcaagacaa
     3841 ccgaagcatt caaattatct acaactacat ctttgaataa agcacctaca tcaaaagcaa
     3901 aactgaaagc ggaaccttcc gtttccgtta aaccagttcc aggacccagg taagagtata
     3961 acaatttaaa gttacctccc aatccaatct tttccggaac tacttcataa ccgtaaccta
     4021 aagcaccggc aacttcataa gtatgaaaat caccgagctc aacattttga gcattggttt
     4081 gaggttgtgt tccttcatcc aaccaaataa tatgggcatt gatgttacca attccataaa
     4141 aatagttatt aaagcccaaa tattcataga agatgtcatc aaccccagaa ccttgtaacc
     4201 aaggtatgtg agttgctgca atttgagtat gacggttaaa agctgttgct cctggattcc
     4261 accacattgc ataagcgtca tctgcaatgg cggaatatgc acgtcccata gcattggcac
     4321 gagctcctgg ttcaaaggtt aagaataaca ttgttgcctg agaaattcca gacaaggcaa
     4381 aaggta
//
LOCUS       DAOPFL010000110         4321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_27597_length_4321_cov_121.104313, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000110 DAOPFL010000000
VERSION     DAOPFL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27597_length_4321_cov_121.104313"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1569
                     /locus_tag="PLG69_06715"
     CDS             178..1569
                     /locus_tag="PLG69_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43236.1"
                     /translation="MPKNFIRPLIIVVLAVILVEILARVIPAVDNHFFPLKKLSWFES
                     LKREKEVVSADTSFVQKAVAISNDLLPLKPFITKLNNPSANLRIAYYGDSIIEGDLIT
                     GKLRMELQSMHQGSGVGMVGITSIVAGFRQTIKHSFSRNWESISFMTPHKYDIPLGIT
                     GYTFIPRNYYVAEKVIEPLQLDSLAVLDSAQVARPEPQKESARFYVDYDPWVEYSASQ
                     VAGGASTFNRIRLFYSHSSDSSYVKVSYNGAPFILRHLQNQPGVQVLDLSPVTACKKI
                     RLEFNAYDPIHLYGVSFDEPQGAYVDNYPIRGYSGMYFQRISSDILSGFQKHLHYDLI
                     ILQYGVNVSNYQIRDYSYYAKGMKKSIEHLQKALPGVPILLISAHDRCIKQGTEYVTS
                     PDIPYLVSTQAKIAKETGCGFWNLFSAMGGLNSMPKYVNHQPPLAGKDYTHFTRAGAD
                     HIAEMLLAYLKGN"
     gene            1570..2868
                     /locus_tag="PLG69_06720"
     CDS             1570..2868
                     /locus_tag="PLG69_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPI43237.1"
                     /translation="MHIRRLLPFCMLALVCIFPVSKSYAEENITIEDINELFSEEEYP
                     SDMDTLEEFIQSYYLLLESNEYLKQYKFLNLDKNQFLGDKKALKPFYEKLLELRSGLR
                     QTVTIYQIGDSHIQAGYFSGTARTSLQQYFGNAGRGLVFPLRLAGTNQPDDYRISASG
                     SFTRLNSERGLSGYALKMADGLCLEIATNNFFRGDNSFNKLSLLTSLPGMETKVEFAS
                     AEPEKNAEKIGNYYYSRVYWETEVSKVKLTLTGESSTLYGICLEKEQPGLLYHSCGIN
                     GAGFYNLLEQHILFEQIGLLNPDLIVISLGTNDAQGTFRGEVFTKNLNTFMYKLRQFN
                     PETPVLFTLPPDSNKRGKHNSDLDKVDKIIIDYAKENQCAWWNLNEVMGGNNSIQKWR
                     NQQMSAKDLLHFTPKGYMLQGYLFYQAFIKSYKSFSEQSG"
     gene            2881..>4321
                     /locus_tag="PLG69_06725"
     CDS             2881..>4321
                     /locus_tag="PLG69_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPI43238.1"
                     /translation="MPPLIQNILNSLVYDPQKPLLFHSTFFLFFFIVVLLFYPLVVNK
                     VKVRTWYLMLASLYFYYKTSGSFIILLLITSLINFGLGAFLYNAQSKATKRFYLWLSV
                     FWNLGSLGYFKYTNFIITTINQVFGGQLPLYDIFLPVGISFFTFQTWSYALDIYFGKL
                     KPLKSFVDFTFFVSFFPQLVAGPIVRASYFIPQIHKKLSLDKDTIAKALILIFSGLIK
                     KGVIADYLSVNFVDRIFENPALFSGLENLLGVYAYALQIYCDFSGYSDIAIGLAALLG
                     FSLPVNFLTPYRASSVTDFWRRWHISLSTWLRDYLYIPLGGNRKGKIRQYLNLMITML
                     LGGLWHGASWNFVVWGGIHGLALVLDKMLLHLKIMQTKVMKVIGVIVTFHFVCFGWIF
                     FRSPNFFSAGMMLNRIFTAFNGRIFWQWLTQYKICAILIITGFVFHWMPDSYNNALQK
                     TLSKAPVILQSLLLAIVIWVLFQFRSAEIQ"
BASE COUNT         1253 a          783 c          854 g         1431 t
ORIGIN      
        1 tttttacaaa gagaaaagag aaaaagagat ttaatgaata gtgaaaaatg aagataactc
       61 ggaaaccata aaaaatctca gtgctctcgg ttttttcggt gttctcggtg taagggaaag
      121 caaaattata aatctgtgtt atctgtaaga gacattatta taacttaaaa ggtggttatg
      181 cctaaaaatt ttatcagacc acttataata gtggttttag cggtcatttt agtggaaatt
      241 ttagccaggg ttattcctgc tgtggataac catttttttc ctttaaaaaa gctttcttgg
      301 tttgaatctc tgaaacgcga gaaagaagtt gtctcagcag atacctcctt tgtgcaaaaa
      361 gcggttgcta tttccaatga tttattaccg cttaagccat ttattaccaa actcaataat
      421 ccttcggcaa atttacggat tgcctattat ggagattcta ttattgaagg ggatctaata
      481 accggaaaac tgcggatgga acttcaatca atgcatcaag gttccggtgt aggaatggta
      541 ggtataactt caattgtagc aggtttcagg caaactatca agcacagttt ttcccgcaac
      601 tgggaaagca tttcctttat gactcctcat aaatatgata ttcctttggg aattaccggt
      661 tacactttta ttccgcgtaa ttattatgta gcggaaaagg ttattgagcc cttacagctt
      721 gatagtttag ctgttttgga ttcagcacag gtagcaagac cggaaccgca aaaagaaagt
      781 gcccgttttt atgttgatta tgacccttgg gtggaatata gtgcatctca ggtagcagga
      841 ggagcttcaa ctttcaatcg tattcgtctt ttttatagtc acagttcgga tagcagttat
      901 gtaaaggtct cctataatgg agctcccttt atcttgcgtc atttacaaaa tcagcccggg
      961 gttcaggttt tagacctcag tcctgttact gcctgtaaaa aaatccggct ggaatttaat
     1021 gcttatgatc ctattcattt atatggagtt agctttgacg aaccgcaagg agcttatgta
     1081 gataactatc ccattcgcgg ttattccgga atgtattttc agaggataag ttctgatatt
     1141 ttatccggtt ttcagaagca cttgcattac gaccttatca tcctgcagta tggagtgaat
     1201 gtttccaatt accaaatacg ggattacagc tattacgcaa aggggatgaa aaaaagcata
     1261 gagcatcttc aaaaagcgct tcccggagtt ccgattttac ttatcagcgc acacgataga
     1321 tgtattaagc agggaacgga gtatgtaact tctcctgata taccttatct ggtttcaact
     1381 caagccaaaa ttgccaaaga aacaggttgt ggtttctgga atttattttc tgcaatgggc
     1441 gggctgaatt ctatgccaaa atatgtaaat catcaacctc ctttggcagg aaaggattac
     1501 actcatttta cacgggcagg agctgatcat attgctgaaa tgcttcttgc ttatctgaaa
     1561 gggaattaag tgcatatccg tcgtttatta cctttttgta tgctggcact ggtttgtatc
     1621 tttccggtaa gcaaaagtta tgccgaagaa aatattacga ttgaagatat caatgaactt
     1681 ttcagcgaag aggaatatcc ttccgatatg gatactctgg aagaatttat tcaaagctat
     1741 tatttgcttc tggaaagtaa tgaatacctg aaacagtata aatttctaaa cctggataaa
     1801 aatcagtttc tgggcgataa aaaagctcta aaaccatttt atgaaaaatt actggaactg
     1861 cgtagtggct taagacaaac ggttactatc tatcaaatag gcgattctca tattcaagca
     1921 ggatattttt ccggaactgc cagaacttca ctccaacaat atttcggcaa tgcaggcagg
     1981 ggattagttt ttcctttacg actagcagga acaaatcaac cggatgacta tagaatatca
     2041 gccagcggct ctttcacccg tttaaattct gaacgcggat taagcggtta tgctttaaaa
     2101 atggcagacg gtttatgcct ggaaatagct accaacaatt ttttcagagg agataacagt
     2161 tttaataaat tatctctgct aacctcgcta cccggaatgg aaacaaaagt ggagtttgct
     2221 tccgctgaac ccgaaaagaa tgccgaaaaa ataggcaact actattacag cagagtttac
     2281 tgggaaacag aagtatccaa agtaaaactt acattaaccg gtgaatcctc tactttatac
     2341 ggaatttgtt tggaaaaaga acaaccagga ttgctatatc actcttgtgg aattaacgga
     2401 gccggttttt acaatctgct ggagcaacat atattgtttg agcagatagg acttttaaac
     2461 cccgatttaa tcgttatttc attaggaaca aacgatgctc agggcacttt tcgcggagag
     2521 gtctttacta aaaacttgaa tacctttatg tataaactaa ggcagttcaa tcccgaaaca
     2581 ccagttcttt ttactcttcc tccggattct aacaagcggg gaaagcacaa tagcgatctg
     2641 gacaaggtgg ataaaattat cattgactat gccaaagaaa atcaatgtgc ctggtggaat
     2701 ttgaacgaag tgatgggcgg caataacagt attcaaaaat ggcgcaacca acaaatgtct
     2761 gccaaagacc ttttgcattt tacccccaag ggctatatgt tgcagggcta tctcttctat
     2821 caggcattta ttaaaagtta taaaagtttc agcgaacaat cgggataaga gaaaattact
     2881 atgcctccct taattcaaaa tatcctgaat agtttagttt acgacccgca aaagccattg
     2941 cttttccaca gcactttctt tctgtttttc tttattgtgg ttttgctttt ctatcctctg
     3001 gtggtgaaca aagttaaagt ccgcacctgg tatctgatgt tggcttcttt atatttctat
     3061 tacaaaacca gcggtagttt cataatttta ttgcttatta cttcgctcat caattttggt
     3121 ttgggtgctt ttctttacaa tgcacagagt aaagccacca agcgttttta tttgtggctt
     3181 agtgttttct ggaatttagg ttcgctgggt tacttcaaat acaccaattt tataattact
     3241 actatcaatc aagtttttgg cgggcaactt cctctttatg atatcttttt acctgtaggc
     3301 atttcctttt ttacttttca aacctggagt tatgctctgg acatctattt cggcaaacta
     3361 aaaccgctga agagttttgt ggatttcacc tttttcgttt cttttttccc gcagctggta
     3421 gctggaccta ttgttcgtgc cagttatttt atccctcaaa ttcataaaaa gttaagttta
     3481 gataaagata ctatagccaa agctctgatt ttgatctttt cgggtttaat taaaaaagga
     3541 gttatagcgg attatttatc ggtcaacttt gtagaccgca tttttgaaaa tccagcgctt
     3601 ttttccggct tggaaaatct tttaggtgtt tatgcttacg ctttacagat ttattgtgat
     3661 tttagcggtt attccgatat tgccatcgga ttggcagcac tattgggttt ttctttaccg
     3721 gtaaactttt taactccata cagggcaagt tctgtaacgg acttttggcg gcgttggcat
     3781 atttctcttt ccacttggtt gagggattat ctgtatattc ctttgggagg aaaccgtaaa
     3841 ggtaaaatca gacaatatct taacctgatg ataactatgc tcttgggtgg tttgtggcat
     3901 ggagcatcct ggaattttgt tgtttggggt ggcattcacg gtttggcttt agttttggat
     3961 aagatgctgt tacacttaaa gataatgcaa acaaaagtga tgaaagttat cggcgtaatc
     4021 gttactttcc attttgtctg tttcggttgg atatttttcc gttcacccaa tttttttagc
     4081 gcaggaatga tgttgaaccg catttttacc gctttcaacg gaagaatttt ctggcagtgg
     4141 ctaactcaat ataaaatatg tgctattctt attataaccg gttttgtgtt tcactggatg
     4201 ccggatagtt ataataatgc tttacaaaaa acgcttagta aagcacctgt aattttacag
     4261 tcgttgcttt tggcaatagt gatttgggtt ttattccaat ttcgctctgc tgaaattcaa
     4321 c
//
LOCUS       DAOPFL010000111         4258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_28156_length_4258_cov_110.660956, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000111 DAOPFL010000000
VERSION     DAOPFL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28156_length_4258_cov_110.660956"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /gene="mutL"
                     /locus_tag="PLG69_06730"
     CDS             <1..1706
                     /gene="mutL"
                     /locus_tag="PLG69_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPI43239.1"
                     /translation="MNADDAMLAFERHSTSKIKTVEDITHIGSLGFRGEALPSIASVS
                     KLHLVTRTAEEEMATIVEYANGKLNNVTKTSANIGTAVTVRGLFKDLPARRKFLKTAA
                     VEARHIIKYIHYQALVYPQISFRLVIDDKEKLNYIACPERKQRIEEVFGSGFFADDVI
                     PVEGKYNDYSVSGYIFGLEDRADKLIDAQYTFINGRYIFDKTVRHSIKAAYEPFILKT
                     RAWQKGTTPPYILFLEIPPEQIDVNVHPAKLEVRFRENNLVHSLVLETLTKALRNYED
                     QKFASARSKFQGGVKGEKVTSLERDIFVKNIEVPRFSAYKKEFGNLFQDDLFRSEPSS
                     TSPKDMPIFNPAQEENTEQEIFPDQPNDQRKYDILLKNEEDYINPWQFHNTYIFIQVE
                     DGLVIIDQHAAHERIIYEKLIQRSEGAPAVRQKLIIPLVIDIPPYISAEIKDLVEENM
                     ELLEKVGFTLKQFSGNSLVIEEIPAELTDFASGQVFIEILKQLEKEMEITTDFRECLA
                     KSIACKSAIKANHKLTRKEMLNLINQLFACQTPYFCPHGRPLMLKIPVTDFEKKFKRT
                     L"
     gene            1703..2584
                     /gene="miaA"
                     /locus_tag="PLG69_06735"
     CDS             1703..2584
                     /gene="miaA"
                     /locus_tag="PLG69_06735"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPI43240.1"
                     /translation="MIPVITIEGATASGKSALAIVLAEALNTEIISADSRQVYRYLDI
                     GTAKVTKEEQKRVKHHLIDIINPDETYNAGAFVKDASIIIEKLHSEGKIPVICGGTGL
                     YIKALLKGLFSLPPLPQEIRQNLKQRLKEEGLAALYAELKSLDPLFAEKISENDTQRI
                     LRGLEVAIGTGIPLSEHWQKQKSSCKYNAFRILIDIPRPELYQRINQRIEKMLAQGLL
                     AEIENLFALGYDENSPGLNCLGYKEFLPYFKKEAGLEECILLAAQHQRNYAKRQVTWY
                     RKGNFDLVISPSGITTF"
     gene            2781..3626
                     /locus_tag="PLG69_06740"
     CDS             2781..3626
                     /locus_tag="PLG69_06740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPI43241.1"
                     /translation="MQIIAKVFEFEISEQELERECAKVSLAEREKCLEGALKRLIDRC
                     LLLYKAMESGIKISDTEYENAIWELLDEEDPLKLWSTSLEQLTPQEMEKLLKQRLMIN
                     KYINSIFPQNIPITNAKIKEFYDEQKEYFLKPEQVRCSHILIRNDNEEAKAKAEQIRR
                     EIHNADDFTYFCQKYSDCPSNNVCGDLGWFPRGKMIPEIEEVAFSLKVGEISQPFLSP
                     YGYHILMKTGQSEKEYIPFEDIKDSLYARLQQIEKEYKLLRHLAELRKQYASHIVIYN
                     ANYLH"
     gene            3949..>4258
                     /locus_tag="PLG69_06745"
     CDS             3949..>4258
                     /locus_tag="PLG69_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43242.1"
                     /translation="MPKHIILTLALTLGIGLLCAFQPVKYAGFCFSGSYTDISKNYPY
                     TQIILQESDSPQSYLDKLFYDYFSANPELGNFTLDIEHKTKATLSLAMALNREEVAFE
                     Y"
BASE COUNT         1403 a          809 c          817 g         1229 t
ORIGIN      
        1 gaatgaatgc tgatgatgca atgctggctt ttgaacgcca ttctaccagc aaaataaaga
       61 cggtggaaga tattacccat atcggttccc tgggttttag aggtgaagca ttaccctcaa
      121 ttgcctccgt ttccaaatta catttggtca ccagaactgc cgaggaagaa atggctacga
      181 ttgtagaata tgctaacggc aaattaaata atgtaactaa gacctctgcc aacatcggaa
      241 ctgcagtaac tgtgcgcgga ttattcaaag accttccagc aagaaggaaa tttttaaaaa
      301 cggcagcagt ggaagccagg cacattatta aatatattca ttatcaagca ttggtttatc
      361 cccaaatcag ttttcggttg gtgatagacg ataaggaaaa gctgaattat attgcctgcc
      421 cagaaagaaa acagcgtatt gaagaggtct ttggcagtgg tttttttgca gatgatgtta
      481 ttcccgtaga aggaaaatat aacgattatt ccgtaagcgg ttatatcttt ggcttggaag
      541 atagagcaga taaactgatt gatgcacaat acacttttat caacggacgc tatatttttg
      601 ataaaacagt gcgtcacagc attaaagctg cctatgaacc ttttatctta aaaacccgcg
      661 cctggcaaaa aggaactaca cctccctata ttcttttttt ggaaattccc ccggaacaaa
      721 ttgatgtgaa tgttcatcct gccaaactgg aagtccgttt tcgggaaaat aatctggtgc
      781 attctttggt tttggaaacg cttaccaaag cactccgcaa ttatgaagac caaaagtttg
      841 ccagtgcacg cagtaaattt caggggggag taaaaggaga aaaagtaact tccctggaac
      901 gcgatatctt tgttaaaaat atagaagttc cccgtttttc cgcctacaaa aaggaattcg
      961 gaaatctctt tcaggatgac ctttttcgta gtgagccaag ttccacttcc cccaaagata
     1021 tgcccatttt taaccctgct caagaagaaa acacagagca ggagattttt cctgaccagc
     1081 ccaatgatca acggaaatac gatatcctgc tgaaaaatga agaggactat atcaatccct
     1141 ggcagtttca caatacctat atttttatcc aggttgaaga cggcttggtg attattgacc
     1201 agcatgcagc tcacgaaaga ataatttatg agaaattgat tcaacgcagt gaaggagctc
     1261 cagctgtgcg tcaaaaactg attattccct tagttattga catcccaccc tatatttctg
     1321 ccgagattaa agacctggtg gaagaaaata tggaactttt ggaaaaagtg ggtttcaccc
     1381 ttaaacaatt tagtggaaac tcactggtaa tagaagaaat tccagctgaa ttgactgatt
     1441 ttgccagcgg tcaggtcttt attgaaattt taaagcagct ggaaaaggaa atggaaatta
     1501 ctactgattt cagagagtgc ctggcaaagt caattgcctg taaatccgct ataaaagcca
     1561 atcataaact tacccgcaaa gaaatgctga atttaatcaa tcagcttttt gcctgtcaaa
     1621 caccctattt ctgtcctcat ggcagacctc tgatgctaaa aattccggta accgactttg
     1681 agaaaaagtt caaacgcact ttatgattcc cgttatcact attgagggag caacggcttc
     1741 cggaaaatcc gcattggcta ttgttttggc agaagcatta aacacagaaa ttatttctgc
     1801 cgattcccgt caggtttacc gctatctgga tatcggaact gccaaagtaa ctaaagaaga
     1861 acaaaaaaga gttaaacatc atttaattga tattataaat cccgatgaga catataatgc
     1921 tggggctttt gttaaagacg cctctataat aattgaaaaa ttgcattccg aaggtaaaat
     1981 tcctgttatt tgcgggggca caggtttata tataaaagct ctcttaaaag ggcttttttc
     2041 tcttcctccc ctgcctcagg aaatcagaca aaatttgaaa caacgcctta aagaagaagg
     2101 acttgcagct ttatatgccg aattgaaaag tttagaccct ctgtttgcgg agaaaataag
     2161 cgaaaatgat acccaaagaa tcttgagagg attggaagtt gcaataggaa ccggaattcc
     2221 cctttccgaa cactggcaaa aacaaaagag cagctgtaaa tataatgctt ttcgtatttt
     2281 aattgatatt cctcgtccgg aactttacca gcgcatcaat cagcgcatag aaaaaatgct
     2341 tgctcaaggt ttactggctg aaattgaaaa cctttttgcc ttaggttacg atgaaaattc
     2401 accaggtctc aattgtctcg gttataaaga atttctgcct tatttcaaaa aagaggcagg
     2461 tttggaggaa tgtattctgt tggctgctca gcatcaacgc aattatgcca aaagacaggt
     2521 tacctggtat cggaaaggca attttgattt agttatttct ccgtctggaa ttactacttt
     2581 ttaatattcc ggaaaaacga tatccccgtc gttctcatta aagaaaaaag cggagcttca
     2641 gcaagctccg acaacacaaa aagtaggttg atgaggatat caaccttcct gttttttttt
     2701 gatttgacaa ctgcctcttc tttgattaaa ttatcaactg taatctcaag gataaaggat
     2761 gatcaaaact gaggtggtaa atgcaaataa tagccaaagt atttgaattt gaaataagcg
     2821 agcaagaact ggaacgcgaa tgtgctaaag taagtttggc agaacgcgaa aaatgtctgg
     2881 aaggtgctct taaacgcttg attgatagat gccttttgct ttataaagca atggaaagcg
     2941 gcattaaaat tagcgatacc gaatacgaaa atgctatatg ggaacttctg gatgaagaag
     3001 accctttaaa actctggagc acttctttag aacaactcac accccaggag atggaaaaac
     3061 ttttgaaaca gcggttgatg attaacaaat acatcaattc tatcttcccg caaaatatcc
     3121 ccatcaccaa tgccaaaatt aaagagtttt atgatgaaca aaaggaatat ttcctgaaac
     3181 cggaacaggt gcgttgctct catattctta ttcgtaacga taatgaagaa gcaaaagcca
     3241 aagcggaaca aattcgtcgg gaaattcata acgctgatga ctttacctac ttttgccaaa
     3301 agtattccga ttgcccaagt aacaatgttt gtggcgattt gggttggttt ccccggggca
     3361 aaatgattcc cgaaattgaa gaagttgctt tttctcttaa agtgggtgaa atcagccaac
     3421 ctttcctgtc tccttacggt tatcacattt taatgaaaac agggcaaagc gaaaaggaat
     3481 acatcccttt tgaagatatt aaggattcgc tttatgcccg cttacaacaa attgagaaag
     3541 aatacaagtt gctcaggcat ttggctgaat tacgcaaaca atatgcatcc cacattgtaa
     3601 tctataatgc aaattattta cactaattag gaaagtaaaa ccgcgctatg aggaatcttt
     3661 cacactaagc tttattaaca aagagagaaa aagatttaat cttacaccga aaatcccgaa
     3721 ttaagttagt ggttgtaact gtctcaatag cttttacctt tgttccaagt ttatttggca
     3781 gtgaggacta attaccggtt aaataaattt aatatatcct cataacttta tagctttata
     3841 acttcttttt tcccgcaatc aaagttcttg acagaaatag gtattgaaac ataaaaggat
     3901 aaatgcccaa tatgctattc tccaaaacgg gagagaggta attttaatat gcctaaacac
     3961 ataattttaa cattagcatt aactttggga atagggcttt tatgcgcttt ccaaccggtt
     4021 aaatatgcag gtttttgttt tagtggttct tataccgata taagcaaaaa ctatccttac
     4081 acccaaataa ttttgcagga gagtgattct cctcagtcct atttggataa actattttac
     4141 gattattttt ccgccaatcc cgaactggga aattttactt tggatataga acataaaaca
     4201 aaggcaactc tttctctggc aatggctttg aatagggaag aggtcgcttt tgaatatt
//
LOCUS       DAOPFL010000112         4163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_29029_length_4163_cov_103.380721, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000112 DAOPFL010000000
VERSION     DAOPFL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29029_length_4163_cov_103.380721"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..2501)
                     /locus_tag="PLG69_06750"
     CDS             complement(48..2501)
                     /locus_tag="PLG69_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPI43243.1"
                     /translation="MILTDAQLFSEISRCEFCENKPCKNACPCDCSPADFMMAAAKGR
                     PSDFKRSAGLILASNPLGGICGNVCPDDHCVNACARENFDTPIQIPAVQATIIRKARE
                     LNMVPEFTPPFSLNKKIAIIGAGPSAIGAAVTLTQFGYQTVMFDPEPLGGQVNLIPEE
                     RLEKIDLLDDLEFLAETFKIEHIRKKIEEPQTLLNDDFEAVIVAGGLNKPIHLNIPGD
                     ETAIYGFDILKNPSQYNFTGKRVALVGGAIAADMALLIAKNNAAYVEMITLENFTEMP
                     LTEPEKATLIEAGIEFSPRTRITEIVNEGNQIKGIKTKKVYLPKGEKFAPNLIRDEEG
                     TTESFRPFDMVIIAIGNKPPFDVNKLETLSDRLFCCGDMINGPTTVVEAVASGKNTAL
                     KVHSLLSKKDISEPEKATKSCFPVWGWKKYPVSLETDFFGRKMDSPFILSAAPPSDGY
                     EQMKKAYLAGWSGGIMKTAFDNEDIHIPGEYMFTFGDKKKTFANCDNVSEHPLDRVCS
                     EIALLIKEFPDRLTMASTGGPVTGDDINDKKGWQANTIKLEKAGAMGIEYSLSCPQGG
                     DGTKGDIVAQDPALTAKIIDWVMEISNPEIPKLFKLTGAVTSIYPILSAVKSVLDKYP
                     GKKAGITLANTFPALAFREKEKPWDEGIIVGLSGEGILPISYLNLARAGNSGIFISGN
                     GGAMNYLDAANFLALGTKNVQFCTIVEKYGYGIIEELKMGLSYLLEAKGLKSVAELIG
                     IAQPKPITDFLDLPTKTKSSSLIKELCLKCGNCTRCPYQAIELDEDCYPKIDPDKCVG
                     CSLCTQLCFTGALEMKNEK"
     gene            complement(2715..>4163)
                     /locus_tag="PLG69_06755"
     CDS             complement(2715..>4163)
                     /locus_tag="PLG69_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPI43244.1"
                     /translation="TAEQKALGRKIVAVQGLGFVGCVMATVVADATDKDGNPLYFVHG
                     HQRASQRSYWKVPVINSGIPPVNSSDPEVPAIFHRTVVEKKNFRATSEESVYGLVDIV
                     VVDIQLDATKPAFGDAEKGYCDITAFREGIRTLGKHIQPDCLVLVETTVPPGTCEKVV
                     KPILEEEFTKRGIDIEKNPPLIAHSYERVMPGAHYVASIRDFWRVFSGVNPKSIELCQ
                     EFLSNVLDVENYPLTQLDSTNASELAKTMENSYRAVNIALTLEWAKFAEQIGVDIFKV
                     RDAIRKRKGTHDNLLRPSLGVGGYCLTKDPVLANWAMKSLFGVEGSLDFAIKAVNTND
                     TMPLHTIDIVKSVYPDLKNLKIAVLGVSYLEDVGDTRHSPSKTLVEFLRKDLAIVKTH
                     DPYVESWPEMEENHIQNDLNNVLPESDVVIFAVGHSQYRKLDPKYVVDLCKTKPLIVD
                     CSNFLSDETINIYKDLGCKVRGVGKGHIVNEK"
BASE COUNT         1222 a          915 c          727 g         1299 t
ORIGIN      
        1 ccattcactg ttcatcattc actgttcacc tttcactttt acatttttca tttttcattc
       61 ttcatttcca gcgctccggt aaagcacaat tgtgtacaca gtgagcatcc aacacattta
      121 tccgggtcaa ttttaggata gcaatcctca tctaattcta ttgcctggta aggacagcga
      181 gtgcagtttc cgcattttag gcacaattct ttaattaaag aagagctttt tgtctttgtg
      241 ggcagatcaa gaaagtccgt aatgggtttt ggctgggcga ttccaataag ttcggcaacg
      301 ctttttaagc cctttgcttc caagaggtaa gaaagtccca ttttcaattc ttcaattata
      361 ccatagccat atttttccac aatagtgcag aactgcacat ttttagttcc caaagctaaa
      421 aagtttgctg catccagata gttcattgca cctccgtttc cggagatgaa aattccgcta
      481 tttccggctc gggctaaatt caaataactg attggcaaaa tgccttcacc gcttaaacca
      541 actatgattc cttcgtccca gggtttttct ttttctctga aagctaaagc aggaaaggta
      601 ttagccaaag ttattccggc ttttttacca ggatatttgt ccaagacact tttaacagcg
      661 gaaagaatag gatagataga agttacagca ccggttagtt taaacagttt gggtatttca
      721 ggattgctaa tttccatcac ccaatctata attttagcgg ttaaagcagg gtcttgagcc
      781 acaatatcac cttttgttcc atctccaccc tgaggacagg ataaactgta ttcaattccc
      841 attgcgcctg ctttttccag ttttatagta ttggcttgcc agcccttttt atcattaata
      901 tcatcaccgg taacaggacc gcctgttgaa gccatagtta agcgatcagg aaattccttt
      961 attaaaagag ctatttcgga acaaactcta tccaaaggat gctctgaaac attatcacaa
     1021 ttggcaaagg tctttttctt atcaccaaag gtgaacatat attcacccgg aatatgaata
     1081 tcttcgttat caaaagctgt tttcataatt ccaccgctcc agccagcaag atatgctttt
     1141 ttcatctgtt catagccatc ggaaggagga gcagcagaaa gaataaaagg ggaatccatt
     1201 tttctcccaa agaaatcggt ttcaagtgaa acaggatatt ttttccagcc ccaaacaggg
     1261 aaacagcttt tagttgcctt ttcgggttca ctaatatcct ttttgcttaa tagagaatga
     1321 acttttaaag ccgtgttttt acctgaggca actgcttcca ccacagttgt aggaccattt
     1381 atcatatctc cacagcaaaa aagacggtct gaaagggtct ctaatttatt aacatcaaaa
     1441 ggaggtttgt tgccgatagc aataataacc atatcaaaag gacgaaagga ttctgtggtt
     1501 ccttcttcgt ccctaatcaa atttggggca aacttctctc ctttaggaag atagaccttt
     1561 ttcgtcttta ttcctttaat ctgattacct tcattaacta tttccgtgat cctggtgcga
     1621 ggagagaatt caattcctgc ctcaatcaaa gttgcctttt ccggttccgt taagggcatt
     1681 tcagtgaaat tttccagggt tatcatttcc acataagcag cattattttt ggcaatcagc
     1741 aatgccatat cggcagcgat tgctccacct acaagagcaa cgcgtttacc agtaaaatta
     1801 tattgggaag ggttttttag aatgtcaaaa ccataaatag ctgtctcatc cccaggaata
     1861 tttaagtgaa tgggcttatt taaacctcct gccacaatta ccgcttcaaa gtcatcattt
     1921 aataatgttt ggggttcttc tatttttttc cgaatatgtt ctattttgaa tgtttctgcc
     1981 aggaattcca ggtcatccag caagtcaatt ttctccaaac gctcttccgg aattaaattt
     2041 acctgacctc ctaaaggttc aggatcaaac attaccgtct gataaccgaa ttgggttaaa
     2101 gttacggcag ctccgatagc agaaggtcct gcaccgataa tggcaatttt cttatttaag
     2161 gaaaagggag gagtaaattc tggcaccata tttaattctc tggctttacg aattattgtt
     2221 gcctgaacgg caggaatttg aattggagtg tcaaaatttt cacgcgcaca ggcatttacg
     2281 caatggtcat cgggacaaac atttccgcaa attcccccta aaggattgct ggctaaaatg
     2341 agtcctgcag atctcttaaa atcggaaggt ctgcctttag cagctgccat cataaaatcg
     2401 gcaggagaac aatcacaggg acaggcattt ttgcagggct tgttctcaca aaattcacaa
     2461 cgacttattt cactgaataa ttgggcatca gttaaaatca ttttatcctc caatattcat
     2521 agccaccgag agcatagaag ttaccgaaaa agataggagc acgcagatgt ctctcacaga
     2581 tagcacagat ttcacagata tttcatttta taattacttt cctaatataa tccagtaaat
     2641 ccattctatc cagttatcta tgttctatca ttcattcacc attctcaatt ttacattttt
     2701 acattctgca ttgttcattt ttcattcaca atatgtcctt ttcccacacc ccgcactttg
     2761 catcctaaat ctttatatat attgatggtt tcatcggaaa ggaaattgga gcaatccaca
     2821 atcaagggtt tggttttaca taaatcaacc acatacttag ggtcaagttt tctatattga
     2881 ctgtgtccta ccgcaaaaat aactacatcg gattcgggaa gaacattatt caggtcattt
     2941 tgaatatggt tttcctccat ttcgggccag ctttctacat aaggatcgtg ggttttcact
     3001 attgccaggt ctttccgcaa aaattccact aaggttttgg aaggagagtg tcttgtatca
     3061 cctacatctt ccaaatagga aacgcctaac acagcaatct tcaggttttt taaatcagga
     3121 taaacgcttt taacgatatc aattgtatgt agcggcattg tgtcattcgt gttaacagct
     3181 ttaatagcaa aatccaaact tccctctaca ccaaaaagtg acttcattgc ccaattagct
     3241 aaaacggggt ctttggttaa acaataaccg ccaacaccta atgaaggacg caacaaatta
     3301 tcatgcgttc cttttctttt tcgaatggca tcgcgcactt tgaaaatatc caccccaatc
     3361 tgttccgcaa atttagccca ttccaatgtg agagcaatat ttacggcacg atatgaattt
     3421 tccattgttt tggcaagttc gctggcattt gtgctgtcca attgagttaa aggataattt
     3481 tccacatcca aaacattgga aagaaattcc tgacagagtt ctatactttt agggttaaca
     3541 ccggaaaaaa cacgccagaa atcacgaata gaagccacat aatgagctcc gggcataact
     3601 ctttcataag aatgagcaat caagggagga ttcttttcta tatctatacc ccgcttggta
     3661 aattcctctt ccagaatggg cttaacgacc ttttcacagg ttccaggagg aacggttgtt
     3721 tctactaaaa ctaaacagtc gggctgaata tgttttccca aagtccgaat tccttcccga
     3781 aaagcggtta tatcacaata acctttttct gcatctccaa aagcgggttt cgttgcatcc
     3841 agctgaatat ctacaactac aatatctacc aaaccataaa cactttcttc gctggtagcc
     3901 cgaaaatttt ttttctccac tacagtgcga tgaaatattg ccggaacttc gggatcggaa
     3961 gaatttacag gcggaatgcc actattgata accggaactt tccaatacga tctttgtgaa
     4021 gcacgctgat gaccatgaac aaaatataaa gggttgccat ctttatctgt agcatcagca
     4081 acaacagtag ccatcacaca acctacaaat cccaaacctt gaactgctac tatttttcgt
     4141 cctaaagctt tttgttcggc agt
//
LOCUS       DAOPFL010000113         4033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_30285_length_4033_cov_98.523881, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000113 DAOPFL010000000
VERSION     DAOPFL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30285_length_4033_cov_98.523881"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLG69_06760"
     CDS             complement(<1..209)
                     /locus_tag="PLG69_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43245.1"
                     /translation="MDAKEIRKKAQLQKAREAQKTPPAYQKPVPRPLFEDETMRPQNI
                     VPVKLNRWIAFGVFLVVLIVYMCTQA"
     gene            complement(222..1208)
                     /locus_tag="PLG69_06765"
     CDS             complement(222..1208)
                     /locus_tag="PLG69_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPI43246.1"
                     /translation="MPEKIRMTIIYTVLTICGLAMIIPFIWMLSTSLMTQSEFNKQES
                     TFIPKESYYVWDNGTQKQKIILVQEKGNTSIIHILNKEDKIIEEYKEVPSSQIKMIKK
                     VPSFHWQNYVKAFNKVPFIIYFKNTLVVSFLTLIGVLITSTLAAYAFARMEFLGRDFL
                     FYLFLSMMMVPEPIYLVSSYVLLDKISWLDTYQALIVPWCVNIFTIFLFRQHFKSLPQ
                     ELFDAAAIDGCSTFGMLWRIMLPLSKSVIATASIFSLIGSWNSFMWPLVMTNRPELRV
                     LQVGLSYFNQEASTQTTLLMAASTFSIVPILILFFMAQKQIIASYAKAGLKD"
     gene            complement(1224..2156)
                     /locus_tag="PLG69_06770"
     CDS             complement(1224..2156)
                     /locus_tag="PLG69_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPI43247.1"
                     /translation="MKTKRKISPYLYILPALFLLVAFRLIPIIMSFVISFFDWSITGT
                     GKFIGLLNYANMLKDSVFWQSMGNTFWLVIFVVPLSIIFSLLFAVLLNNIKAVKGLFR
                     TIYFMPYVTSLVAVSIVWKLMFNEQTGLINTLIGYIGIPPQKWLSESRGIFEMLFARA
                     GTSFPRFLGGPSQALFAIIIMTVWKGLGYNTIIYLSGLQNISKVYYEAAEIDGASKIK
                     QFFKITLPLVSPTTFYVLIMTTIVTFQTFSQVYLMTDKGGPLNTTKLIVYYIYEKGFD
                     TLEMGYASAVALVLFIIILALTIYQRRLEKYVNY"
     gene            complement(2260..3471)
                     /locus_tag="PLG69_06775"
     CDS             complement(2260..3471)
                     /locus_tag="PLG69_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPI43248.1"
                     /translation="MELSKRAREIQASPIRKLNPYANSAKKRGIKVYHLNIGQPDLPT
                     PKEMLKAYHEFSDEVLEYGPSQGLDIYREGLVKYYGNLGIELKVDDIIVTTGGSEAIT
                     FAMMVVCEVGDEIIVPEPFYTNYNGFATMAGITLIPLTTYAENGFALPGNSVIEAKIS
                     PKTKAIMLCNPGNPTGTVYSREEIFRIADLAKRKGLYFISDEVYREFIYDGLSHTSVL
                     EVPDFEENAILVDSVSKRYSACGARIGCIVSKNKEIMSSVLKFAQARLCPPTVDQLVA
                     NAGIYLPETYFEQLKVEYQSRRDLVLSELEKIPGVICKKPEGAFYVFAKLPIDDAEDF
                     IIWMLKEFQIDNETVMAAPGDGFYATPGLGKNELRLAYILRKEDLKKAMYIFQNGLAE
                     YQKLHIGKASS"
BASE COUNT         1283 a          799 c          704 g         1247 t
ORIGIN      
        1 gcttgagtac acatataaac aattaaaacg acaagaaaga ccccgaaagc tatccaacgg
       61 ttcaatttta ccggcacaat attttggggg cgcattgttt catcctcaaa aagaggacga
      121 ggaaccggtt tttgataggc aggaggtgtt ttttgtgcct ctctggcttt ctgtaattgt
      181 gctttttttc ttatttcttt ggcatccact tttttctcct tttaatcctt taatcctgct
      241 ttagcatagc tggctataat ttgtttttgt gccataaaga acagaatcaa tatggggaca
      301 atgctaaagg ttgaagcagc cattaaaagt gttgtttgcg ttgatgcttc ctgattaaag
      361 tagctaagcc caacttgcaa gacccttaat tcaggacggt tagtcattac cagtggccac
      421 ataaaactgt tccagcttcc gattaaagaa aaaatggaag cggtagcaat aaccgatttg
      481 gaaagaggaa gcataattct ccagagcata ccaaaagtac tgcagccgtc aatagcagca
      541 gcatcaaaaa gttcctgggg gagtgatttg aaatgctgac ggaagaggaa aatagtaaaa
      601 atgtttacac accaagggac tataagtgcc tgataggtat ccagccaact gattttatcc
      661 aacaaaacat aggaagaaac cagatagatg ggttccggaa ccatcatcat acttaaaaat
      721 agatagaata aaaagtccct tcccaaaaat tccattcggg caaaagcata agcagcaagg
      781 gtagaagtaa tcagcactcc gattaaagtt aagaaactaa ctaccagtgt atttttaaag
      841 tagataataa aaggaacttt gttaaatgcc ttaacataat tctgccagtg aaaactggga
      901 actttcttaa tcatttttat ttgggaactg gggacttcct tatattcctc aattatttta
      961 tcttctttat tcaagatatg aatgatgctg gtattgcctt tttcctgcac cagaataatt
     1021 ttttgctttt gagttccatt gtcccaaaca taatatgatt ccttaggaat aaaagtgctt
     1081 tcctgtttat tgaattcgga ttgtgtcatt aaagaagtgg aaagcatcca gatgaaaggg
     1141 ataatcatag ccagtccaca aatagtaaga actgtgtata tgatggtcat acgaatcttt
     1201 tccggcattt ccttcctcct tccttaataa ttaacatatt tttccaaccg tctttgatag
     1261 atggttaaag caaggataat gatgaaaaga actaaggcaa cagcactggc ataacccatt
     1321 tccaaagtgt caaatccctt ttcataaata taatatacaa tcagtttggt agtatttagt
     1381 ggtccgcctt tatccgtcat taaatagacc tgggaaaagg tctggaaagt aacaatcgtt
     1441 gtcataatca gcacataaaa agtagtaggg gagaccagag gcagagttat tttgaaaaat
     1501 tgtttgattt tactggcgcc atcaatttcg gctgcctcat aatagacctt ggaaatgttt
     1561 tgcaaaccag aaaggtaaat aattgtatta taacccaagc ccttccaaac agtcataata
     1621 ataattgcaa aaagcgcttg ggaaggtccc cctaaaaagc ggggaaaaga tgttcctgcc
     1681 ctggcaaaca gcatttcaaa tatacctctg gattcggata accatttttg aggtggaatt
     1741 ccaatataac caattaaggt atttattaag ccggtttgct cattgaacat caatttccag
     1801 acgattgaaa cagctaccag ggaagttaca tagggcataa aataaatcgt gcggaaaagt
     1861 cctttaacag ctttaatgtt gtttaacagc accgcaaaaa gcagagaaaa aataatgctt
     1921 aagggaacta caaaaatcac cagccagaat gtattgccca ttgattgcca aaaaacgcta
     1981 tctttcaaca tattagcata attaagcaag ccgatgaatt tgcctgtgcc tgtaattgac
     2041 cagtcaaaaa agctgattac aaagctcatt atgattggta ttaaacgaaa agcaaccagc
     2101 aaaaacaaag ccggcagaat atagaggtag ggagagattt ttcgtttcgt tttcatacta
     2161 ttgtcctttc atttctcctt tatcgggatt tatcagtttc ctttttgctt tggtatcctg
     2221 aaaatcccct tccattttct tcattttttc cctttaatat tatgaacttg cttttcctat
     2281 atgcaatttc tgatattcgg caagtccatt ttgaaaaatg tacattgctt ttttcaggtc
     2341 ttctttacga agaatataag ccaggcgaag ttcatttttc cctaatccag gtgtagcata
     2401 aaatccgtca cccggagccg ccataaccgt ttcattatca atctgaaatt ccttcagcat
     2461 ccagataata aagtcctctg catcgtcaat aggtagcttg gcaaacacat aaaaagcccc
     2521 ttccggtttt ttacaaataa caccaggaat tttttccagc tcacttaata ccaaatcacg
     2581 ccgagattga tattctactt ttaactgttc aaaatatgtt tccggtaaat aaattccggc
     2641 atttgccacc aattgatcca cggttggagg gcataaacgc gcttgagcaa actttaatac
     2701 cgaggacatt atttccttgt ttttgcttac aatacaacca attctggctc cacaagcact
     2761 atagcgttta gaaacactat ccactaaaat agcgttttct tcaaaatcgg gaacttcaag
     2821 aacgctggta tggcttaatc catcataaat aaactcccgg tagacctcgt cagaaatgaa
     2881 atagagacct ttgcgttttg ccaaatcagc aatgcgaaaa atttcttctc tactgtaaac
     2941 agttcccgtt ggatttcccg gattgcaaag cattatggct ttggttttgg gactgatttt
     3001 tgcctctatt accgagttcc caggtaaggc aaaaccattt tccgcataag ttgttaaagg
     3061 tatcaaagtt atccctgcca ttgtagcaaa accgttatag tttgtataaa acggttcagg
     3121 aacaataatt tcgtctccaa cttcgcaaac aaccatcatc gcgaaagtta ttgcttcgct
     3181 accaccggtt gttacaataa tatcatctac ctttaattca atacctaaat ttccgtaata
     3241 tttaactaaa ccctcacgat aaatatccag accttgggaa ggtccatatt ccaaaacctc
     3301 atctgaaaat tcgtgatatg ccttcaacat ttctttcggg gtgggcaaat cgggctgacc
     3361 aatattcaga tgataaacct taattcccct ttttttcgcg gaattggcat aaggatttaa
     3421 tttacggatg ggtgatgcct gaatttctcg ggctcgttta gagagttcca ttttttgttt
     3481 cctttatatt ttattttata ttataatttt caactggact gtaatgtatg taggcagcta
     3541 ctatctccaa cttttttttc cggaaggttg aattcctcgt caaccagctt tttcgctttt
     3601 tcttcttgcc gaaaagctta taataacgca agaactggct tctaagagga acaacaggat
     3661 gtcgttcttc cgaaaaaatt tcgccagaga ttaccattcc ttcgcttcta tatttctctt
     3721 ccgataccta ctatttacaa tcctgaaaaa agacaagaaa aatctttgtg catacaaaga
     3781 aaatattatc tacttaaaca taaataatgc agttgaggaa aaatgggttg gcaaattcca
     3841 aattgctgaa aagttcgcag cactctgaaa aataattgtc cataatttat ggggaattag
     3901 aattcagcaa acaaaaagca aaaattgact tttcttctcc gggttgaaac ccttctataa
     3961 tatctgtcgt tcctacggaa ctttttctcg gaaaaagaga taattactat tctgcattca
     4021 tttcaccggg ttc
//
LOCUS       DAOPFL010000114         3930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_31386_length_3930_cov_81.400000, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000114 DAOPFL010000000
VERSION     DAOPFL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31386_length_3930_cov_81.400000"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3089
                     /locus_tag="PLG69_06780"
     CDS             <1..3089
                     /locus_tag="PLG69_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPI43249.1"
                     /translation="TMFLTDLSINRPVLVTMAIMVFIVFGVLAYFSLPINLMPDLKLP
                     YVVVQTVYSGAGPREIESQVTEPIEEAVATVSQIDFMQSYSMENVSIVLVAFKLGKNI
                     DVANQEVKDKVDAVIRNLPDGTDRPIVLKMDITAFPIMDLVLSGNMPPKELYELADGT
                     LKDRLSQIPGVASVSLIGGAKRQIDVRFTDKVVFENKISLAQLSAILAAANLDMPGGN
                     FTQGTQEYSVRLKGEYQDVEDIRNTDIPTVFGVKKLSQLARVEDATEEVRSKAIYFNV
                     PENVKDENIVRLSITNAADGNVVTIAEEVTKEIPILNKELPEGVKLEIMRDDSIFTKD
                     TINSTLENILLGILFTGLILFIFLHDFRSTIIVAISMPYCIISTFVFMQIFGYTFNIM
                     TMMGLSTAIGILVSNSVVVLENIFRHKDMGNTRKVAAQIGTNEIGTAVLASTLTNIVV
                     FLPIATMTSMVGRFFKEFAVTVTMATIFSLLTAFTITPMLASKIIPKEKRTSKWGRKF
                     DNVFDKFGNYYAKFLGYTLRSKKTSVSILLFTLLALLITFGLAFFVGMEMMPPVDQRN
                     LSISVEMPQGTNLEQTAKTMDIIQNRLSQHKEVVHILTNLGSGSFIDTGTNLATSDIK
                     LVDKNERKYTANQLADILTKELADIPNAKIKVTGSMLGMGGGGRSGLQFMIEGQDNAR
                     LEQLKNEVTKAIEDIPGLINLDTSSRYGRSELTLYPKRDKLAAAGATVYDLALALRAN
                     VEGLVSTKYREAGNEYDIKISLEDEVVDTPDKIKNLSVVVMGKSYLVSQLADIQFSSG
                     INRITHYDRYKTVMFTGDVASGYTMSDITSQIEERLDKIKFPTGYRYQWGGQAKMMSE
                     TTVDMIRTFILAVILTYMLLAAILESFAQPILILATVPLALIGVILSLLITGNTFNIV
                     SMMAIIMLVGIVVNNAILIMDYVNIKRREGYSVHDALMEAGKMKLKPIVMSTLAIIAG
                     MLPMAIGIGSTGSEMTRPMGIVSIGGLIVSTFLTLIIIPAFYFLTTKNIHAKKESQ"
     gene            3112..3627
                     /locus_tag="PLG69_06785"
     CDS             3112..3627
                     /locus_tag="PLG69_06785"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HPI43250.1"
                     /translation="MKRITRLVSIALLAVFIVVSVFGCYGNMSLTKKVYRFNGSVGNK
                     FVQSIVNWAFLICPVYEIAMFLDVFVFNTIEFWTGSNPLTMNEGENIIKYAEGPNGNY
                     KFEISQNKIVISQPDNKDNNQKIELNYDPVSESWFMNSNGTTQKIGYLDGNQLKLLSQ
                     SGEEISLDLYK"
     gene            3637..3864
                     /locus_tag="PLG69_06790"
     CDS             3637..3864
                     /locus_tag="PLG69_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPI43251.1"
                     /translation="MIAKEKIESILAKVRPSIQADGGDVELINIREDNVIEVRLKGTC
                     NGCPMATLTLKAGIERLIKEEIPEVKEVIAV"
BASE COUNT         1198 a          707 c          821 g         1204 t
ORIGIN      
        1 gaactatgtt tttaacagat ctgtctatta accgaccagt tctggtcaca atggcaataa
       61 tggtgtttat cgtcttcggg gtgctcgctt acttcagttt gccgataaac ctgatgccgg
      121 atttgaagtt gccttatgtt gttgttcaga ccgtatattc aggagccggt ccccgcgaaa
      181 ttgaatctca ggtaaccgaa ccgatagaag aagcagttgc cacggtcagc cagattgact
      241 ttatgcaatc ctactcaatg gagaatgtct ctattgtgct ggttgccttc aaattgggta
      301 agaacataga tgttgccaat caggaagtga aggataaggt ggatgccgtt atccgtaatt
      361 taccagatgg cacggacaga cccatagtcc tgaaaatgga tattactgct tttccgatta
      421 tggacctggt gctttccgga aatatgcctc cgaaggaatt atacgagctt gcggatggga
      481 cgctaaaaga tcgtttatcc caaattcctg gtgtagcaag tgtctcgtta attggaggag
      541 ccaagaggca aatagatgtt cggtttaccg ataaggttgt gtttgaaaat aaaatatcac
      601 ttgctcagtt aagtgcaatt cttgctgcag caaatttgga tatgccgggt ggtaatttta
      661 cccaaggaac tcaggagtat tcagtccgct tgaaagggga atatcaagat gtggaagata
      721 tcagaaatac cgatataccc actgtttttg gagtgaaaaa actttcacag ctggcaagag
      781 ttgaagatgc tacagaagaa gtgcgttcca aagcaattta ttttaatgtt ccggaaaatg
      841 ttaaagatga aaatatagtc cgtctgtcta taaccaatgc tgccgatgga aatgttgtaa
      901 ctatcgctga agaagtcacc aaagaaattc cgattttaaa taaagaactt ccggaaggtg
      961 taaaactgga aattatgcgg gatgatagca tatttaccaa agatacgatt aattctaccc
     1021 tggaaaatat tctcttgggt attctcttta cgggacttat tctcttcatc tttttacacg
     1081 atttccgttc tactattatt gtagcaatat caatgcccta ttgcataatt tcaacctttg
     1141 tgtttatgca aattttcggc tatactttca atattatgac gatgatgggt ctttctaccg
     1201 caataggaat tctggtttcc aactcagtgg tcgttttgga gaatattttc cggcataaag
     1261 atatgggaaa tactagaaaa gttgcagctc aaattggaac caatgaaata ggcacagcag
     1321 ttctggcatc tactttaacc aatattgtag tatttttacc tattgctaca atgacttcta
     1381 tggtgggacg cttcttcaag gaatttgctg taaccgttac tatggcaacc atattttctc
     1441 ttttaaccgc ttttacgatt acacctatgc tggcttccaa gattattccg aaagagaaac
     1501 gcaccagtaa atggggaagg aaatttgata atgtctttga taaatttggt aattactatg
     1561 ccaaattctt aggatataca ttgcgttcca agaaaaccag tgttagtatc cttcttttta
     1621 ctctattggc tttgctgatt acttttggcc ttgctttctt tgtgggtatg gaaatgatgc
     1681 ctcctgtgga ccaacgcaat ctatccattt ctgtagaaat gccccaggga acaaatttgg
     1741 aacagacagc taaaacaatg gacatcatcc aaaaccggct atcccaacat aaagaagttg
     1801 tccacatact cactaatctc ggttcaggca gctttattga taccggcacc aatttggcta
     1861 cttccgatat taaactggtg gataaaaatg aacgtaaata tactgctaat caattagcag
     1921 atatactaac caaagaactt gccgatattc ccaacgccaa aattaaagtt accggctcaa
     1981 tgttaggtat gggaggaggt ggcaggtctg gtttgcaatt tatgattgaa ggccaggata
     2041 atgcccgttt ggaacaactg aaaaacgaag taaccaaagc tattgaagat ataccgggtt
     2101 taatcaatct ggatacttca agccgttatg gcaggtctga acttaccctt tatcccaaac
     2161 gcgataaact tgccgctgcc ggagctacgg tttatgattt ggctttagct ttgcgcgcta
     2221 atgtggaagg acttgtttct accaaatatc gtgaagccgg aaatgaatat gatatcaaga
     2281 tttctcttga ggatgaagta gttgatactc ccgataaaat taaaaacctg agtgtagttg
     2341 tgatgggtaa atcctatttg gtatctcagc tggcagatat tcaattttct tccggtatta
     2401 atagaataac tcactacgac cgctataaga ccgttatgtt taccggtgat gtagccagtg
     2461 gatacactat gagcgatata acttcccaaa ttgaagaacg attggataaa attaaatttc
     2521 cgaccggcta tcgttatcaa tggggaggtc aggcaaaaat gatgagtgaa actacagtag
     2581 atatgattcg cacttttatt cttgctgtga ttttaaccta tatgctctta gcggctattc
     2641 tggaaagttt tgctcaacca attctgattt tggcaacggt tcctctggct ttgattggag
     2701 tgatactttc cttactcatt acaggtaata catttaacat cgtttctatg atggctataa
     2761 taatgttagt tggcattgtg gtaaataacg ctatattaat aatggactat gtaaatatta
     2821 aacgcagaga gggctatagt gtccacgatg cgttgatgga agcgggaaag atgaaattaa
     2881 aaccgatagt aatgtctacc ttagccatca tagcgggaat gcttcctatg gcaattggta
     2941 taggtagtac gggatccgaa atgacccgtc cgatgggtat tgtgtctatt ggtggtttaa
     3001 ttgtttccac ttttctaacg ctgattatta tccccgcttt ctatttcctg accactaaaa
     3061 atatccatgc taaaaaggaa tcccaataaa cccaataaaa aggagagaaa aatgaaaaga
     3121 atcacgcgtc ttgtctctat cgctttatta gcggtattta ttgttgttag cgtcttcgga
     3181 tgctatggga atatgtcact taccaaaaaa gtgtatcgct ttaatggtag tgtcgggaat
     3241 aagtttgttc agagcattgt taactgggct tttttgattt gtccggttta tgaaatagca
     3301 atgtttctgg atgtttttgt ttttaatacc attgaatttt ggaccggtag caatcctctc
     3361 actatgaacg aaggtgaaaa tattatcaag tatgccgaag gtcccaatgg gaattataag
     3421 tttgaaatca gtcaaaacaa aattgtgatt tctcaacctg acaataagga caacaatcaa
     3481 aaaattgaac tcaactatga ccccgttagc gaatcctggt ttatgaacag caatggaacc
     3541 acccaaaaaa ttggttattt agatggaaat caattaaaat tgctttctca atctggtgaa
     3601 gaaatctcct tagacctcta taaataagga taaaaagtga ttgcaaaaga aaaaattgaa
     3661 agtatcctcg caaaagttcg cccctccatt caagcggatg gaggggatgt tgaacttatc
     3721 aatatccgtg aagataatgt gatagaagtt cggcttaaag gaacttgtaa tggttgccct
     3781 atggcaactt taactttgaa ggcaggcatt gaacgactta ttaaagaaga aatccctgaa
     3841 gtgaaagaag ttatcgctgt gtaattaact ggagggaaag tcaggagtca ttagcgcctt
     3901 gataacttct tatatgggag ataactttct
//
LOCUS       DAOPFL010000115         3914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_31567_length_3914_cov_94.957761, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000115 DAOPFL010000000
VERSION     DAOPFL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31567_length_3914_cov_94.957761"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3914)
                     /locus_tag="PLG69_06795"
     CDS             complement(<1..>3914)
                     /locus_tag="PLG69_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPI43252.1"
                     /translation="LVLYANRPMDTQYFSSSDDFKAQTVGTNRARKLYSDGTTYDPMN
                     PSATGTLSGTFPKTSLTFVVTGMGSLSGTVTSSGSPVADVLVQVSNANYSFTRYTNAA
                     GEYSFQYLPIGNYTVTASKLGYETQSVTVTIIENQNTVQNFSLVSSNTVSVSGHIVGS
                     DQPTVGIANAHIFLDGALDYEGISNANGDFTITGVLSGNTYNYTIQAIGYADLTGTVV
                     VGSTNVNMGTLVMSELALPPVQIVAEENAAQTQVTLTWRPPGSTGTGVGTEDFEIDDG
                     GWVSSGYGDWQWGHYNVANYVDIDTYTDTPPQSAHSGTGMWGTVLEGGYSNCNTWSYL
                     RKTFNLQSVSNPVLSFWHYMNGYNTWDYGLIKVNGNTVWGNSSSAVFMPWQELTINLS
                     AYANLSEVEISFEWFATGTVSYAGWYIDDLYVGPSMNKTVNYTYVPIPEKAHLLSEEE
                     EALLQPKDFPPVNRSFSSPNSQISNPNRALNGYKVWRFIFGNENNENSWTLLTANTIT
                     DTTFIDTAWGTLPDGNYRWAVKGVYTNNLLGPAGFSNRILILRNDLAANTITGNTTPS
                     VGTSFNYTVTIENMGTETKPAGSYTVKLMSGDTELASVLGPTIAAGEELAVTIPFTPT
                     TEGTMSLTGKVVLPADTNPSNDITPPINLTVMPAGILAVTIGEGGQLVGIPWEFYYKN
                     SLFQTLYYSNEIGLFGNITAISFYNNFVSNLTDKPVKLWLGTTNLQDLSGGWILDGLT
                     LVYDGTLNFPSGQNTITVSLQTPYLYTGGNLVLYANRPMDTQYYNTNDDFQAQTIGSN
                     RALKIQSDSVTYDPMNPSVAGTLSGQFPKTTLFFSSPGPNPIFSVGPSSHNWGTVLIN
                     TVNEQNFVVGNAGGGTLTINSITISGSEMFSLQNLPALPANLAFCETINFTARYNPTA
                     VGNHTATITITDNLATTYTITLNKSNNDGDKRISHNVSLSGNCIDTTLNILPYAQNFD
                     QVTVPALPLDWMKIVQSTSTSAVVETYASTTYAHSQPNCVRLYNPSDANATLMLIAPP
                     LGTAIPTNTTRVKFWARSSSAGYPISIGIIVNPTDPSTYQETELISLTTTLTEYVVAF
                     NAYTGTGKHIVFKHGLGGTSRLLYVDDVMIEIIPTNDLAATAISGNVTPSVGQESQYN
                     ISIHNWGTASQNTYSVKLFSATGAELATAAGITCAPGATVDVPINWTPTTEGAMTIYG
                     KVFLVGDQNSLNDQTPNLNILVNPSGVFMITIGTGGQFARMPVDMYYRNSIYEGLYYP
                     AEMSNFMGQITGIQFYNNFYTDLPNMPTKVWIGTTTLTSLSD"
BASE COUNT         1194 a          772 c          830 g         1118 t
ORIGIN      
        1 catccgacaa agaagttaaa gtggtagttc cgatccagac cttagtaggc atattgggca
       61 gatcggtata gaagttgtta tagaactgaa taccggttat ctgacccata aagttactca
      121 tttcggcagg ataatatagc ccttcataga tgctgtttct ataatacata tcaacaggca
      181 tccgagcaaa ctgacctcct gttcctatgg taatcataaa gaccccggaa ggatttacca
      241 gaatatttaa gttgggtgtt tggtcattca aactattttg gtctccaacc aagaaaactt
      301 tgccgtaaat agtcattgcc ccttcagttg tgggagtcca gttaatcgga acatctactg
      361 tagctccggg cgcacaagtt attcctgctg ctgtagcaag ttcagcacca gtagcagaga
      421 acagcttaac cgaataggta ttttgggaag ccgttcccca attatggatg gaaatattat
      481 actgcgattc ttgtcctacg gagggagtta cattaccgga gatagctgtt gcagcaagat
      541 cattggtcgg aataatttct atcataacat catctacata aagtaagcga ctggttccgc
      601 ccaaaccatg tttgaagacg atatgttttc ctgttcccgt gtatgcattg aaggcaacta
      661 catattctgt gagtgtggtg gttaaggata tcagttcagt ttcctgataa gtggaaggat
      721 cggtaggatt tactattatt cctatagaga ttggataacc agcactggaa ctgcgagccc
      781 agaattttac cctggttgta ttcgtaggaa ttgccgtacc caagggtgga gcaatcaaca
      841 tcaaagtggc attggcatca gaaggattat acaagcgaac acaattgggc tgactgtgag
      901 cataagtagt agaagcataa gtttccacta ctgctgaggt ggaagttgat tgcacaattt
      961 tcatccagtc cagaggtaaa gcaggaaccg taacctgatc aaagttttgg gcgtaaggta
     1021 aaatattcaa tgtggtatca atacaatttc cggaaaggga aacattatga gaaatccgtt
     1081 tatccccatc attattactt ttgttgaggg ttatagtgta agttgttgcc agattatccg
     1141 taatcgtaat agtagccgta tgattcccaa cagcagtagg attgtatctg gctgtgaaat
     1201 taatggtttc gcaaaatgca agattggctg gcagagcagg taaattctgc aaagagaaca
     1261 tttcactgcc actgattgta atactattga ttgtaagagt tccacctcca gcattgccaa
     1321 caacaaagtt ctgctcgttc acggtattaa ttaacactgt tccccagtta tgagaagaag
     1381 gacctacact aaagatagga tttggtcccg gagaggagaa aaagagtgtt gttttaggaa
     1441 attgacccga tagtgtgcct gcaacagaag gattcattgg atcgtaagta acggaatcgg
     1501 attggatttt aagagcacgg ttggaaccta ttgtctgtgc ttgaaaatca tcattagtat
     1561 tataatattg ggtatccata ggtctattag catagagaac caaatttcca cctgtataga
     1621 gataaggtgt ctgcagggaa actgtaatag tattttgacc gctggggaag tttaaggttc
     1681 catcatatac aagagttaaa ccgtccaaaa tccaaccgcc actgagatcc tgcagatttg
     1741 tggttcctaa ccacagttta accggtttat cagttaaatt agaaacaaag ttattataga
     1801 aagaaatagc agttatattt ccgaataaac cgatttcatt agaataatag agggtttgaa
     1861 acaaactgtt cttatagtag aattcccagg gaatacctac taactgtcca ccctctccaa
     1921 tagttactgc taaaattccc gcgggcataa ctgttaaatt tattgggggt gtaatatcat
     1981 tactgggatt ggtgtcggca ggaagaacta cttttccggt taagctcatt gtgccttctg
     2041 tagtaggagt aaagggaata gtaacagcaa gttcttcacc tgcagcaata gtaggtccta
     2101 aaacacttgc cagttccgta tctccactca taagtttaac tgtatagctg cctgccggtt
     2161 tggtttctgt tcccatattt tcaattgtta ctgtatagtt aaaagaagtt ccaactgaag
     2221 gtgttgtatt gccggtaatt gtattggctg ccaggtcatt tctcaggata agtatccggt
     2281 tggaaaagcc cgcaggacca agtaagttat tagtataaac acctttcact gcccaacggt
     2341 aatttccgtc cggtaaagtt ccccaggcag tatcaataaa ggttgtatcg gtaatagtat
     2401 tagcagtaag caaagtccag gaattctcat tattttcgtt accgaaaata aaccgccaaa
     2461 ccttgtaacc atttaatgct ctgttcggat ttgatatttg agaattgggg gaagaaaatg
     2521 agcgattaac tggcggaaaa tctttaggtt gcaataatgc ttcttcctct tcagaaagca
     2581 aatgtgcttt ttcgggaata ggcacataag tataatttac ggttttgttc atagagggac
     2641 cgacatagag gtcatcaata taccatccgg cataggaaac agtgcctgta gcaaaccact
     2701 caaaacttat ttccacttca ctcaggttag cataggcaga aagatttata gtaagttcct
     2761 gccatggcat aaagacagcg gaagaactgt tgccccaaac agtattgccg tttactttga
     2821 ttaaaccata atcccaggta ttgtaaccgt tcatatagtg ccaaaagctc aatacgggat
     2881 tagaaacact ttgtaaatta aaggtttttc ttaaataaga ccaggtatta caattggaat
     2941 aacctccctc taagacagtt ccccacattc ctgttccgga atgtgcagat tgaggaggag
     3001 tatctgtata agtatctata tccacataat tagccacatt ataatgaccc cattgccagt
     3061 caccgtagcc actagaaacc caaccgccat cgtcaatttc aaaatcttcc gtaccaactc
     3121 cggttcctgt tgagccagga ggacgccagg taagggtaac ttgcgtttga gcagcatttt
     3181 cttctgctac aatttgcacc ggaggaagag ctaattcact cattaccaag gttcccatat
     3241 ttacattagt ggaaccaact accacagttc cggtcaaatc tgcatagcca atcgcctgaa
     3301 tagtatagtt atatgtattt ccggaaagaa caccggttat cgtaaaatca ccattggcat
     3361 tggaaatgcc ttcataatcc agggctccgt ccagaaaaat atgagcattg gcaattccta
     3421 cagtcggctg atcgctacca acaatatgtc cactgacaga taccgtgttg gaagagacca
     3481 aactgaagtt ctgaaccgta ttctgattct caataatagt tacggtaacg gattgagttt
     3541 cataacccag cttggaagca gttacagtat aattgccaat aggcaaatat tggaaagaat
     3601 attcaccagc ggcatttgta taacgggtaa aggaatagtt ggcattgctc acttgaacta
     3661 aaacatccgc aaccggagaa ccactgctgg taactgtccc gcttaaagaa cccattccgg
     3721 ttactacaaa ggttaatgag gttttgggaa aagttccgga aagcgttcct gtagctgaag
     3781 gattcatcgg atcgtaagtt gtgccatctg aatataattt acgggcacgg ttagttccta
     3841 cagtttgagc tttaaaatca tcactgctgc taaaatattg tgtatccata ggtctattgg
     3901 cataaagaac caga
//
LOCUS       DAOPFL010000116         3859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_32252_length_3859_cov_85.436383, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000116 DAOPFL010000000
VERSION     DAOPFL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32252_length_3859_cov_85.436383"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLG69_06800"
     CDS             complement(<1..277)
                     /locus_tag="PLG69_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HPI43253.1"
                     /translation="MLNIVFYFQVHQPYRLNHFNVLDIAHNAKIFNDKLNGDIMRKVA
                     NKCYLPTNQLLLELIEKYEGRFKVAFSISGTALEQFKLYSPETLDSFK"
     gene            complement(288..1631)
                     /locus_tag="PLG69_06805"
     CDS             complement(288..1631)
                     /locus_tag="PLG69_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI43254.1"
                     /translation="MKILMFTWEFPPLISGGLGMACYGMVKALLSQGIKIDLVLPTKE
                     LVYFPLREESDADTLPAVFIEPEKHKEYTKRTFSTLHERLEYIGASTHPESYFNLSEI
                     KDYVSSIKKTFWLTETVTTQEHNIWKEMTAHLIGEEDLIRKVQEYTLRASRFAEILDY
                     DLIHAHDWLTYPSGILAKQISRKPLIVHIHATEFDRAGGPGDERIHKIEHLGMNYADK
                     VIAVSQYTAQMIMSRYRIDTGKIRIIYNAFTVSETTLHNKKRIFKGPTILYLGRITLQ
                     KGPDYYLDMADKVLKVHPEARFILAGTGDMQRQILRRSAALRLKNRFLFTGFLNRKQV
                     ERILQAADIYVLPSVSEPFGISPLEAMAYGITAIISKQSGVAEVVHNAFKIDYWDIDL
                     WAETINHLIENPEECAKIGMEGRKEVQKIQWNEAADKIRQLYAETLSEYSKRMEL"
     gene            complement(1628..3664)
                     /locus_tag="PLG69_06810"
     CDS             complement(1628..3664)
                     /locus_tag="PLG69_06810"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPI43255.1"
                     /translation="MNNYFMETHHHEWILTNRRGGYALGTGNLINQRKYHGLLVSSDS
                     KFNRLHLVAGIEEKVEWRGEFFHLDSNNYSNCIYPEGFLHLVKPWLRPYPIFLYSALP
                     HQNDILILKEIMMDESTNTTLVKYTNLGHHLLHFELHPKFTMIPHHELNKPGTLDFLN
                     FITEIETQDKFTRFSVQRQDNNIKLYGALLSGEVIPNRYVYYNVFYPWEVMSGYEGIG
                     DQITLFQLGFDLRVGQSNYLLFSDTAIEDAKTLIERIENRYADLPKPLDYPYASDKND
                     TLLNNLDYNDNIIFPYDNYLKILEFALKDFLANDDIVAGFPFYGAWGRDTMVVVNALL
                     HCPNQLETVEKILNKYRGYIKNGLIPNMIAESGRETNYDSVDSTLWYIILLWKLGKRK
                     QTVKYWKDVIALTEEIINSFLHNESHPFSVRYDGLIELPPDFAHGTWMDVRIDGKAIT
                     PRNGAPVEINALWYNALCSYEAMCLAYCQKSRKKYQAKEEFVQLKELVKQSLQKFYQD
                     GYLADRIVGEELIMEIRPNAIIALALPWQAFSQEILQQVLERSFKELYTNYGIRTLSP
                     KDIRFHKKYYGTQKERDLAYHNGSVWAWLLGPFCGLYERAYKGIKTKEEIIEALTSFI
                     STFRNSFMKGHIASIAEIWDGDAPHFPKGAPAQAWSVAALYNIENYILFLQEGK"
BASE COUNT         1193 a          763 c          701 g         1202 t
ORIGIN      
        1 gcttgaagga atcaagggtt tcgggagaat agagtttaaa ttgttccaaa gcagtgcccg
       61 aaatagaaaa agcaacctta aacctgcctt catatttttc aataagctcc agaagcaact
      121 ggttagttgg taaataacat ttatttgcta ccttacgcat tatatcgcca tttagtttat
      181 cattgaaaat tttagcatta tgagcgatat caagcacatt aaaatgattt agacgatagg
      241 gttgatgcac ctgaaagtag aatacaatat ttaacatcgc tgtctcctta taattccatt
      301 cgtttgctat actcagaaag cgtttcggca tagagctgac ggattttatc cgcggcttca
      361 ttccactgaa ttttttggac ttcttttctg ccttccattc caattttagc gcattcttca
      421 ggattttcta ttaaatgatt gatggtttca gcccaaaggt ctatatccca atagtcaatc
      481 ttaaaggcat tatgcacaac ttcagcaact ccggattgtt tggaaattat agccgttatt
      541 ccataagcca ttgcttccag aggagaaata ccaaaaggtt cggaaacaga gggtaaaaca
      601 taaatatcag ctgcctgcaa aattcgttca acctgtttac gattcaaaaa accggtgaag
      661 agaaaacggt ttttcaagcg taaggcggca gaacgacgca aaatctgacg ttgcatatct
      721 cctgttccag caaggataaa tcttgcttcg ggatgaactt tcagaacctt atctgccata
      781 tccagataat aatcaggacc tttttgtaaa gttattcttc ctaaataaag aatagtagga
      841 cccttaaaaa tgcgtttctt gttgtgaaga gtagtttccg aaacggtaaa agcattataa
      901 attatgcgga ttttaccggt atctatccga tagcgactca taatcatctg agctgtatat
      961 tgagaaacgg caattacttt atcggcatag ttcattccga gatgttcaat tttatgaatt
     1021 ctttcgtctc caggaccacc tgctctatca aattccgtgg catgtatatg cacaatcaat
     1081 ggcttacggg aaatctgttt ggctaaaatt ccgctggggt aagtaagcca gtcatgcgca
     1141 tgaatcaggt cataatccaa aatttcggca aaacgacttg ctctcaatgt atattcctgc
     1201 actttccgaa ttaaatcttc ttcaccaatg agatgagcag tcatttcttt ccagatatta
     1261 tgttcttggg tggtaacagt ttcagtaagc cagaaggttt ttttaatgga acttacatag
     1321 tccttaattt cactcaagtt aaaataactt tccggatggg tgctggctcc gatatattcc
     1381 aagcgttcgt gcaaagtaga aaaagtccgc tttgtatatt ccttgtgttt ttccggttca
     1441 ataaacacgg caggaagggt atcggcatca ctttcttccc ttaaaggaaa ataaaccagt
     1501 tctttggttg gtaaaacgag gtctattttt attccttgac taagcagagc tttcaccatt
     1561 ccataacatg ccatacctaa acccccggaa atcagaggtg gaaattccca ggtgaacatt
     1621 agaattttca tttaccctcc tgcagaaaaa ggatgtaatt ttcaatattg tataaggcag
     1681 ctacactcca tgcctgagcc ggtgctcctt taggaaaatg gggagcatct ccatcccaga
     1741 tttccgcaat ggaagcaata tgtcctttca taaaactgtt gcgaaaagtg gaaataaagg
     1801 aagtgagggc ttcaattatt tcttctttgg tctttatacc tttataagct ctttcataca
     1861 aaccgcaaaa aggacctaac agccaagccc aaacactgcc attatgataa gcaaggtctc
     1921 tttctttctg tgttccataa tattttttat gaaaacggat atctttagga ctcaaagtgc
     1981 ggattccata gttggtgtag agctctttaa aactgcgttc cagaacctgt tgcagaattt
     2041 cctgagaaaa tgcttgccag ggcaaggcaa gagcaataat tgcattagga cgaatttcca
     2101 ttataagctc ttcacctaca attctatctg ccagatagcc atcttgatag aacttttgca
     2161 gggattgttt taccagctct ttcaactgga cgaattcttc ttttgcttga tatttttttc
     2221 gggatttctg acagtaggca aggcacatag cttcataaga acataaagcg ttataccaga
     2281 gggcattaat ttctactgga gctccattac gaggagttat cgcttttcca tctatccgca
     2341 catccatcca tgtaccatga gcaaaatcgg gaggcaattc aattaaacca tcgtaacgca
     2401 cactaaaggg atgggattca ttatgcaaaa agctgtttat gatttcttcc gttaaggcaa
     2461 taacatcttt ccagtattta accgtttgct tgcgtttacc caatttccac agcaggataa
     2521 tataccataa agtagaatct acactatcat aatttgtttc cctgccggat tctgcaatca
     2581 tattaggaat cagaccattt ttaatgtagc cgcgatattt attgaggatt ttttccaccg
     2641 tttccagttg attgggacaa tgtaagagcg catttaccac aaccattgta tctcttcccc
     2701 aagcaccgta aaaaggaaag ccggctacaa tatcgtcatt ggctaaaaaa tccttcaggg
     2761 caaattccag gattttcaga taattgtcat acgggaaaat aatattatcg ttataatcca
     2821 agttattcaa aagggtatca tttttatcgg aagcataagg ataatccaaa ggtttgggaa
     2881 ggtctgcata acgattttca atcctttcaa tgagagtttt ggcatcttca attgctgtat
     2941 cactgaaaag aagatagttg ctttgtccca cccttaaatc aaaaccaagc tgaaaaagag
     3001 ttatctgatc accaatacct tcataaccac tcatcacttc ccagggataa aaaacattat
     3061 aataaacata gcgattggga ataacttcac ctgaaagcaa agcaccataa agttttatat
     3121 tattatcctg cctctgaaca ctaaaacgag tgaatttatc ctgagtttca atttccgtaa
     3181 taaaattcaa gaaatctaaa gtgccaggtt tattgagctc atgatgagga atcatcgtaa
     3241 acttgggatg aagctcaaaa tgcagaaggt gatgacccaa attggtatat ttcaccaaag
     3301 ttgtattggt ggattcatcc atcattatct ctttcagaat caaaatatcg ttctgatggg
     3361 gaagagctga atacaaaaat atcggatagg gacgcaacca aggttttact aaatgcagaa
     3421 aaccctcagg atatatacaa ttgctgtaat tattgctgtc caggtgaaaa aattctcctc
     3481 gccattcaac tttttcttca atgccagcca ccaaatgaag gcgattgaat ttactgtcgc
     3541 tgctaaccag taaaccgtga tattttcgct ggttaatcaa atttcctgtt cctaaagcat
     3601 aaccgcctct acggttagtt agaatccatt catgatgatg ggtctccata aaatagttgt
     3661 tcatcaaacc tcctcaaatt ctaatcctaa gttgtgttga tttttttcca tattggttat
     3721 ttcgtcaagt ccttttttat aacctctcac agattccact gattttgcag attttgattg
     3781 ataaggggta ggagatgaac aggattttta ggattttttt tacaaagaga aggaatgaag
     3841 aatgaattta aaagaggtt
//
LOCUS       DAOPFL010000117         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_32375_length_3848_cov_105.553124, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000117 DAOPFL010000000
VERSION     DAOPFL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32375_length_3848_cov_105.553124"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLG69_06815"
     CDS             complement(<1..521)
                     /locus_tag="PLG69_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPI43256.1"
                     /translation="MIFDLVYRNRSYRRFHEEKRLTRAQLIELIKVARYIPSAGNLQK
                     LRYFLIYEEDKCDLIFPHLKWASYLKTWNGPEKGERPSAYIIILAPVDSPKMVFIDTG
                     IAAQTILLSAVDNGFGGCMLASVDKQEVQNTFSLPEEMEIMLVIALGYPAESVVIEDV
                     TEQGKIEYWRDEKG"
     gene            complement(1109..3106)
                     /locus_tag="PLG69_06820"
     CDS             complement(1109..3106)
                     /locus_tag="PLG69_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43257.1"
                     /translation="MLKYVLIVLFALLFSGFLNYRTNPEITKSRRWILFALRFITLFL
                     LLILLMSPILYYTQHKKQAPQILVLEDISQSMDLKHNSTTKSQHLKPLWQEMEKKFTA
                     SGYEVLHKTFANGLEGDKNNTLLSPALKELSRQKVFTNLEGVVLASDGWLRDESLSAV
                     QQLGCPFYVLADSSQNPAPDLAIVNVRCNRYAYRNEPNTIRAEISSQNYSGPAEVRLL
                     MANKIISRQNLKLEKGITANVDFTHKFNSIGFFPWKVEVAALPNESQINNNIYPGAIE
                     VLAEKQRIILISDKPAWDNKFTLDAIATNSRWTAESYLNRNGRLYSGEVLVNKLEPDN
                     LAALIIINNGILNLDNATVSFIQNAYNRGVGILYQGLPVNELASLLPIQKSNITSTYQ
                     GFIVPTPISANYPMLNSLTLNAKDIPPLDYYYVTASPGSEILATINNPQNSPAIVVQT
                     TGTARSLAFATLNLWRWQMQSGDDGYTKLISNCLTWLSNKSTGTYNAIYNNSYFRGEE
                     IRIRLRSEDEIRQSRLDVNPRIRILDKNNKEVYTDFLKREGDEYAITLQLTEPGTYSF
                     EISDKETGKSTKGKFELSETSIETRDYGYNLSLLSWLAKETGGKLLYKSNLNTFNPVP
                     AKPVEQISRKEIPLYKKWYILSLFILTFCAELFLRRRWGLL"
     gene            complement(3106..>3848)
                     /locus_tag="PLG69_06825"
     CDS             complement(3106..>3848)
                     /locus_tag="PLG69_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43258.1"
                     /translation="TFAMGNFTLPYIPKTIPPIRAGFSVSALVGIGNANLDDFFGYFS
                     SSMEGVDVKQDIVLHTGLGGFGQKAMLGLASNITPNLEVGLTLDNLFGKINWFLTTEE
                     TTIKVVANDVYAANISEEIYDYANETVDIDSYSTKLPPELRLGTLYKIPWANFSLDYL
                     QGFKTSIATSGIGRFSLAAQFLPWEYLPLNIGVGFGNSHYPWRISYGIGYSSKAVEFG
                     FGLQSVKNLIPGYKTKGIAIGSYMKVHL"
BASE COUNT         1209 a          746 c          684 g         1209 t
ORIGIN      
        1 cctttttcat cacgccaata ttcaattttt ccctgttctg taacatcttc tataacaact
       61 gattctgcag gatagcccaa agcaataacc agcataattt ccatctcttc gggcaaagaa
      121 aaggtatttt gaacttcctg tttatcaact gaagccagca tacatccgcc aaaaccatta
      181 tccacagcgc ttaagagtat tgtctgagca gcaattccgg tatcaataaa caccatcttt
      241 gggctatcaa ccggcgctaa aataataata taagcagagg gtctttctcc tttttcaggt
      301 ccattccagg tttttaaata acttgcccac ttcaaatggg gaaaaatcaa atcgcattta
      361 tcctcttcat aaataaggaa atagcgcaat ttttgcagat tacccgcact gggaatgtat
      421 ctggctacct ttatgagctc tataagttgt gcgcgagtta acctcttttc ttcgtgaaac
      481 ctgcgataac tccgattgcg ataaacaagg tcaaaaatca tctttctctc cttaaaaaaa
      541 gttctatttc tgtgtctata tataattata atgaatactt gcgcctttga ccagacattt
      601 ttggtgacag taatataaat attagattga gaatgtataa atatttattt atctcatttt
      661 gattcctgct ttcgcaagaa tgacaaatgg cttaccaatc aaaaatgata taagctgttg
      721 taaccaaaaa tcaaccccca aattaacagc aggtttaaag caaattcaga tttcggaata
      781 aatgacatct attatatata tctgcttgtt aatcaattga ttatctatct tgcctatcaa
      841 tgttaaattc caacacctaa cttattgata attagtcagt tctctaacga ctccgtaaca
      901 cttccgtaac actgccgtaa tctcgttacg ggatcgttac ttggttgtta ctgaatcgtt
      961 aaattgttca gaaagggaaa aaatatcgtt cactgctggc aaagctataa taggaaagca
     1021 aattaaacgg attttaacaa attcacacgg atttttttat tatcagtcca tcttttttca
     1081 agataaataa ttcattctct attcttcatt acagcagtcc ccatcttctt cttaaaaaaa
     1141 gttccgcaca gaaggttaaa ataaacaggg agagaatata ccacttttta tagaggggga
     1201 tttctttgcg ggaaatttgt tctacaggtt tggcaggaac gggattaaag gtatttagat
     1261 tgcttttata taataatttt ccacctgttt ctttagcgag ccaggaaaga agtgacaaat
     1321 tatagccata atctcttgtt tcaatagatg tctcggagag ttcaaatttg cctttggtgc
     1381 ttttccctgt ttccttgtca gagatttcaa agctgtaagt tcccggttct gtaagttgaa
     1441 gagtgatagc atattcatca ccttcgcgtt tcagaaaatc ggtatagacc tctttattat
     1501 ttttatccag aatacgaatg cgagggttaa catccaaacg actttggcga atttcgtctt
     1561 cactacgcag acggatacga atttcttcac cccgaaaata actgttattg taaatagcgt
     1621 tataagttcc tgtgctttta ttactaagcc aggttaaaca attactgatt aattttgtgt
     1681 aaccatcatc tccgctttgc atttgccaac gccaaaggtt taaagttgca aaagccagag
     1741 agcgtgcagt tcctgtagtt tgaacaacaa ttgccggaga attttgcgga ttattaatag
     1801 tagctaaaat ttcactaccc gggctggcag ttacataata ataatccagc ggtggaatat
     1861 ctttggcatt taaggttaaa ctattcagca taggatagtt agctgagata ggcgtaggaa
     1921 ctatgaatcc ttgataagta gaagtaatat tggatttttg aattggcaaa agagaagcta
     1981 attcgtttac gggaagtccc tgatagagga ttcccactcc gcgattatag gcattttgta
     2041 taaaacttac cgtagcatta tccaaattga gtattccgtt attgataata attaaggcag
     2101 ctaagttatc cggttcaagt ttattaacta acacttctcc gctataaaga cgcccgtttc
     2161 tattcagata gctttctgct gtccaacggc tatttgttgc aatagcatcc agagtgaatt
     2221 tattatccca ggcgggctta tcggaaataa ggataatcct ttgtttttct gcgagcactt
     2281 caatagctcc agggtagata ttattgttaa tttggctttc atttggcaaa gcggcaactt
     2341 ccactttcca gggaaaaaaa ccgatgctgt taaacttatg ggtaaaatcc acattagcgg
     2401 ttatgccttt ttccagtttc aagttttggc gtgagataat tttatttgcc atcaacaaac
     2461 ggacttctgc aggtccgctg taattttgag aggaaatttc agcacgaata gtattgggct
     2521 catttctata ggcataacgg ttacaacgca cattcactat tgccaaatcg ggagcaggat
     2581 tttgagaact atcggctaaa acataaaaag gacagcccaa ttgctgaact gcagataaag
     2641 attcatctcg caaccagcca tctgaagcaa gaactactcc ttccaaattg gtaaatactt
     2701 tttgacgact aagttctttt aaggcaggag acaataaagt gttattttta tctccttcca
     2761 agccattagc aaaggttttg tgaagaactt catagccgct agcggtaaat ttcttttcca
     2821 tttcctgcca aagtggtttt aaatgctgag atttggttgt ggaattatgc tttaagtcca
     2881 tactttgaga aatatcttcc aataccagta tttgaggtgc ctgctttttg tgttgagtgt
     2941 aatataaaat ggggctcatc aacaatatga gcagcaggaa aagtgtaata aaccttaagg
     3001 cgaaaagaat ccatcgcctt gatttggtaa tttcgggatt agttctatag ttcagaaaac
     3061 ctgaaaataa aagtgcaaac agaactatca gaacatattt cagcattata gatgaacctt
     3121 catataactt cctatggcaa ttcccttggt cttgtaaccg ggaataagat ttttaacact
     3181 ttgcaagcca aaaccgaatt ctactgcttt gctgctatat ccgattccat aacttatacg
     3241 ccagggataa tgactgttac caaaacccac accaatattc agaggtaaat attcccaagg
     3301 caaaaattga gcagctaaag aaaatctgcc aattccgctg gtagcaatag aagttttaaa
     3361 accctgaaga taatctaagg aaaaattagc ccaaggtatt ttatatagag tgcccaaacg
     3421 taattccgga ggaagctttg tagaataaga atcaatatct acagtttcat ttgcgtaatc
     3481 ataaatttcc tcggagatat tagccgcata aacatcattg gcaaccactt tgatagttgt
     3541 ttcttcagta gttagaaacc agtttatttt gccaaagagg ttatctaaag tcaaccctac
     3601 ttccaaattg ggagtgatat tacttgccaa tcccaacatt gctttttgcc cgaaaccccc
     3661 cagacctgtg tgtaaaacga tatcctgttt aacatccaca ccttccattg aagaactgaa
     3721 atagccgaaa aaatcgtcca gattggcatt tcctattcct actaaggcac taacggaaaa
     3781 gccggctcta atgggaggaa tagttttcgg aatataggga agagtaaaat tccccattgc
     3841 aaaggtga
//
LOCUS       DAOPFL010000118         3831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_32599_length_3831_cov_105.743379, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000118 DAOPFL010000000
VERSION     DAOPFL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32599_length_3831_cov_105.743379"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PLG69_06830"
     CDS             <1..1377
                     /locus_tag="PLG69_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPI43259.1"
                     /translation="IFETTEFSFDYLTSRLRELAFLNNGLRIILKDERSGRTHDFKYE
                     GGIKSFVEYLNQNKKPLFPNPIYISSIKEGMEFEVALQYNEGYQENIFSFANNINTTE
                     GGTHLSGFKSGLTRAVNTYIKNADLLKNEKVSPSGEDIREGLTAVISLKITNPQFEGQ
                     TKTKLQNSEVEGIVNSAVYEKLMEYFEEHPAEAKNITMKSILAARSREAARKARELTR
                     RKTVLESGSLPGKLADCTLNDPEKTELFLVEGDSAGGSAKQGRDRSFQAILPLWGKML
                     NTEKARVDKVLNNDKIQPIILALGAGIGQEFDISKLRYHKIIIMADADVDGSHIATLL
                     MTFFYRYMRPVIEEGKLYIAMPPLFQVRKGKQKVYVYNEEERDKAIREFGEKGVLVQR
                     YKGLGEMNAEQLWETTLDPKNRKMIAIKMDDAIEADRMFTILMGDEVEPRREFIQENA
                     RYVKNLDV"
     gene            1391..>3831
                     /gene="gyrA"
                     /locus_tag="PLG69_06835"
     CDS             1391..>3831
                     /gene="gyrA"
                     /locus_tag="PLG69_06835"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPI43260.1"
                     /translation="MSETTNIIPVQIEEQLKQAYLDYSMSVIVSRALPDIRDGLKPSQ
                     RRIIYAMHELNLTPGSHFRKCAKIAGDTSGNYHPHGEQVVYPTLVRLAQPWIMRYPLI
                     EGQGNFGSIDGDPPAAMRYTEARLQKITMEMLTELEKDTVEFRSNYDETRQEPTVFPA
                     RFPNLLVNGSSGIAVGMATNMPPHNIGEVCDAIIALIDNPDLQPLDLLEYIKGPDFPT
                     GGFILGTEGIREYFETGRGKIIMRGETEIETLPNDIEQIIIRSIPYQLSTSQLIERMV
                     ELVKDKKIEGISDIRDESGRDGMRIVIQVKRNHDAQVVRNLLYKYTQLQSTFGVINLC
                     LIDGVPQVVNMKEMLQNFIDFRHEVVLRRTQFDLRNAKERLHILEGLKIALDNLDEVI
                     ALIRASQTPNDASVGLQEKFGLSEIQAKAILDMRLQRLTGLERDKIETEYQELLIIIA
                     RLTELVEHKELRMNLIKEETAEIRDKFADKRLTTIIPAPAGSFNMEDLIADEMVVVTI
                     THDGYVKRLPVDTYKVQARGGKGLSASNLKEEDFIQYLFVASTHSYILLFTDHGMCYW
                     LKVFEIPEASRTARGKAIVNLVKLAEGDRIKAFVTLKDFHPEQNIVMCTRNGLVKKTP
                     LTAFSHPRSIGIKAIKLREGDELIDARICEAGDDILLATHEGKCNRFSESEIRSMRRF
                     SQGVKGIRLRNGDYVISMTIATAQDQLENGKPNSSTILAISEKGYGKRSPISSYSVTR
                     RGSMGVITLKTNERNGYLAALMLVRDDDDLMIITQGGMIIRQRVSEISVVGRNTLGVR
                     LINLAPNDVVRDISLV"
BASE COUNT         1231 a          680 c          849 g         1071 t
ORIGIN      
        1 atttttgaaa caacggaatt cagttttgat tatttaactt cccgcttaag agaactggca
       61 tttttaaaca atggattgcg gattatttta aaagatgaac gcagtggtcg cactcatgat
      121 tttaaatatg aggggggaat aaaaagtttt gtggaatact taaatcaaaa caaaaaaccg
      181 ctttttccca atccgatata catttccagc ataaaagaag gaatggaatt tgaagtagcg
      241 ctgcaatata acgagggtta tcaggaaaac atcttcagtt ttgccaataa tattaatact
      301 actgaagggg gaactcattt aagcggattt aaaagcggtt taacgcgtgc cgtgaatact
      361 tacatcaaaa atgccgatct gcttaaaaat gaaaaggtta gtccttccgg tgaagatatt
      421 agagaagggc ttactgcggt tatttcactt aaaattacca atccccaatt tgagggacag
      481 accaaaacca aactgcaaaa ttcggaagtg gaaggaattg ttaatagtgc tgtttacgaa
      541 aagcttatgg agtattttga agagcatcct gctgaagcaa aaaatattac aatgaaaagt
      601 attttagcgg cacgcagtag agaagcagcc agaaaggcaa gagaacttac caggagaaaa
      661 actgtcctgg aaagtggctc cctgccagga aagcttgctg attgcacttt aaacgatccg
      721 gaaaaaacag aactcttttt agtggaaggt gactcggcag gcggaagcgc caaacaagga
      781 agagatcgtt cttttcaagc aatacttcct ttgtggggaa aaatgctgaa cacggaaaaa
      841 gcaagagtgg ataaggtttt gaacaacgat aaaattcaac cgattatttt agctttggga
      901 gctggaattg gacaggaatt tgacattagc aaactgcgtt atcataaaat cattataatg
      961 gctgatgccg atgtagacgg ttctcatatt gccaccttgc taatgacctt tttctatcgc
     1021 tatatgcgtc ccgtaattga agaaggtaaa ctctatattg caatgccgcc tctttttcag
     1081 gttcgcaaag gaaaacagaa ggtttatgtg tataatgaag aagaacggga taaagcaatt
     1141 cgggaatttg gtgaaaaagg tgtgcttgtt caacgctata aaggacttgg tgaaatgaat
     1201 gcggaacagc tttgggaaac cactcttgat cctaaaaacc ggaaaatgat tgctatcaaa
     1261 atggatgatg ccattgaggc ggatagaatg tttaccattt taatgggcga tgaagtagaa
     1321 cccagaaggg aatttattca ggaaaatgcc cgctatgtaa aaaatcttga tgtttaagga
     1381 gcaaaaatag atgtctgaaa caaccaatat tattcctgtt caaatagaag aacaactaaa
     1441 acaagcatac cttgattatt ctatgagtgt gattgtttca cgcgccttac ccgatattag
     1501 ggatggcttg aaaccctctc aacgccgaat aatctatgcg atgcatgaac ttaatttaac
     1561 tcccggaagt cactttcgta aatgtgcaaa aattgccggg gacacaagtg gtaattatca
     1621 tcctcacgga gaacaagttg tatatcctac tttagtgcgt ttggcacagc cctggataat
     1681 gcgttatccg ttaattgaag gtcagggaaa tttcggttcc atagatggtg atccgcctgc
     1741 agcaatgcga tatacagaag cacgcctgca aaaaataaca atggaaatgc ttaccgaact
     1801 ggagaaggat accgttgaat ttagaagcaa ctacgatgaa acccgtcaag aacctactgt
     1861 ttttcctgcg cggtttccca atttactagt aaacggttct tccggaatcg ctgtcggaat
     1921 ggcaaccaat atgccacccc ataatatagg tgaggtctgt gatgccatta ttgctctaat
     1981 tgataatccc gatttgcaac cattggactt gctggaatat attaagggac ccgattttcc
     2041 aactggtggt tttattttgg gaactgaggg aataagagaa tattttgaaa cgggtagagg
     2101 caaaattatt atgcggggtg aaactgaaat tgaaacccta cccaatgata tagaacagat
     2161 tataattcgt tccattccct atcagctttc tacaagtcag cttatagaac gaatggtgga
     2221 gctggtgaaa gacaaaaaaa ttgaaggcat tagcgatatt cgggatgaat ccggacgcga
     2281 tggaatgcgc attgtaattc aggtaaaacg caatcatgat gctcaagtag tccgtaattt
     2341 gctttataaa tatacacagc tgcaatccac ttttggcgta attaatctct gtctgataga
     2401 tggtgttccg caagttgtga atatgaaaga aatgctgcag aatttcattg attttcggca
     2461 cgaagttgtt ttacgccgca ctcaatttga cctgcgcaat gccaaagaac gattgcacat
     2521 tttagagggc ttgaaaatag ctctggataa tctggatgag gttattgctc taatccgtgc
     2581 ttcacaaaca ccaaatgatg ccagcgttgg tttacaggaa aaattcggct taagcgaaat
     2641 tcaggcaaaa gctattctgg atatgcgtct ccaacgctta accggtttgg aacgcgataa
     2701 aatagaaacg gaatatcagg aattgttgat tattattgct cgtcttaccg aacttgttga
     2761 acataaagaa cttagaatga atttaattaa ggaagaaaca gccgaaatta gagataaatt
     2821 tgctgataaa aggttaacta caattatccc tgctccggct ggaagtttca atatggaaga
     2881 cctgattgcg gatgaaatgg tggtggttac tatcactcat gatggctatg taaaacgctt
     2941 acctgttgat acctataaag ttcaagccag aggaggaaag ggtttaagcg cttccaatct
     3001 gaaagaagag gactttattc aatacctctt cgttgccagc actcattcct atatcttgct
     3061 ttttaccgat catggaatgt gttactggct aaaggttttt gaaattcctg aagcaagtag
     3121 aacagccaga ggaaaagcca ttgtgaattt ggtaaaactt gccgaaggag atagaattaa
     3181 agcttttgta actttgaagg atttccatcc cgaacaaaat atcgtgatgt gcacgcgtaa
     3241 cggtctggtt aaaaaaactc ctttaaccgc tttttcgcat cctcgctcca tcggtattaa
     3301 agctatcaaa ctgagagaag gcgatgaatt gattgatgcc agaatttgtg aagcgggaga
     3361 tgatattctt ctggctactc atgagggaaa atgcaatcgt ttcagtgaaa gcgaaatccg
     3421 ttctatgcgt cgtttttccc aaggtgtgaa aggaattcgt ttgcgcaatg gagattatgt
     3481 aatatctatg actattgcca ctgctcagga tcaattggaa aacggtaaac ccaattcctc
     3541 cactatttta gctattagtg aaaagggtta tggcaaacga agccctattt ccagttattc
     3601 tgttaccaga agaggttcta tgggtgttat aaccctgaag acaaatgaac gaaacggtta
     3661 tttggctgcc ttgatgctgg tgcgggatga tgacgattta atgattatca ctcaaggcgg
     3721 aatgattatc cgacaacgcg tttccgaaat ttccgtggtg ggaagaaata ctttgggagt
     3781 gcgtttgata aaccttgctc ccaatgatgt agtgcgcgat atatctcttg t
//
LOCUS       DAOPFL010000119         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_32703_length_3822_cov_87.856915, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000119 DAOPFL010000000
VERSION     DAOPFL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32703_length_3822_cov_87.856915"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..584)
                     /locus_tag="PLG69_06840"
     CDS             complement(6..584)
                     /locus_tag="PLG69_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPI43261.1"
                     /translation="MRTKMIHRSLNTYFHIVNHSIDGRKLYYDPGDYKTYLYLFKKEL
                     DYKVTVIAYCLMPNHFHFLLRQNAPEAISSLLERTHKRYARYYNKKYNFKGRIFRSPL
                     HHIETPTENYLFNACAYIHANPVQAGLVGFPEEWEFSNFREYVRMRKGKLYSEQFLMD
                     YIVNPEKYRLKVIDIAWKKSMQRAFEKDSIWF"
     gene            complement(2042..>3822)
                     /locus_tag="PLG69_06845"
     CDS             complement(2042..>3822)
                     /locus_tag="PLG69_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43262.1"
                     /translation="YEFTVSFVPQTIGEKTATLNILDNLSAKVLHTIALSGTAIAEQI
                     YIPVNLTATVTEQDVQLTWANYSGIPGSPGWLSYDDGTNYDGIGTGSSGTFDVAIRFD
                     SGTMYGYADMELSKVKYFPRSANTTYTIKVWTGNDGTLTPSTLVYSQAHTPTVNQWNE
                     VVLNTPIPISGTQALWIGYECVVATGADYYPAGCDAGPAVVGYGDLISLGTWESMATG
                     YGLDYNWNLQGYFDIPIGTKAPKLLSIPVINQELRSKEHLAVRFTTSGNSNPPNRVLR
                     GYNIYRNAIQINTLLVPTATYLDQGVPYGTYSYTVQGVYYTALSPMSDPVVVNVEPPI
                     PYSLPFIETWSSADFATNQWNTTAANWIIDSGNGLEEPSAAFSWSPQITNYQEYLTSW
                     YLDATGISNVKFKFDMALNNYSTYAENLLIPQVWDGTTWQTFDTFSSFDNEGAGYGWD
                     TYVYDISAYASNRMFRIRFLAAGEDSYEINYWYIDNIVVEAIPATLPQPTLAIDYDGT
                     NVNLTWDPVPGTDWYLIYAATDPYGTYDYLGYWPASYGTALSFTPNSKEFYQIKACSM
                     ELPVIPPTKGYNVFHKKIPKTVANRK"
BASE COUNT         1134 a          707 c          807 g         1174 t
ORIGIN      
        1 gtaagtcaaa accaaatgct atccttctca aatgctcttt gcattgactt tttccatgct
       61 atatcaataa ctttcaatct gtatttttcc ggattcacga tataatccat cagaaattgt
      121 tctgaatata atttcccttt ccgcatccgg acatattccc ggaaattgga aaattcccat
      181 tcttcaggaa aacctactaa acctgcctgt acaggattgg catgaatata ggcacaagca
      241 ttgaagagat aattttccgt tggtgtttca atatgatgta agggactgcg aaatatcctt
      301 cctttgaagt tatatttttt attgtaatag cgtgcatatc ttttgtgagt tctttccagt
      361 aatgaactaa ttgcttcagg tgcattttga cgcaaaagaa aatgaaaatg attgggcata
      421 aggcaatagg caatcacagt tactttatag tccaattcct ttttaaataa gtaaagatag
      481 gttttataat caccaggatc ataataaagt tttctgccat caatagaatg atttacgata
      541 tggaaataag tgttgaggga acgatgaatc atttttgtac gcattcggaa atctcctgtt
      601 ttttattaga tatgataaat actttcggga agaaaacaaa taatactttc ttacacgata
      661 gcggaattcg tgagtttgtt gtagctcggt tgacagtgtg cgggttctgg gtgaaatttt
      721 gacgcgatga tgttttgact ctaaaccgac ccttttgtgt ttgcaacgag attttgaggc
      781 aacaggaaat atatttcatt gtccgatact ttcaaagctg aagctcggtt tagagttaaa
      841 acaaaaattc gccccgaagt cacaaattct gaacaattct taccccacat ttctggagcg
      901 gctgaatata tattttttcc gtgaccgtgt aattcgtgag tttgttgtag ctcggttgac
      961 agtgtgcagg gttcttgttt taactcacaa ttttgaagtt tggagaccat ttgtagttca
     1021 gttgatagtg tttataaatt gagggttgtg tttgccgtga tggctgtggc aaaattgtaa
     1081 gtcaaaacaa gcatcgttgc aactatattt tgaggcaaca ggaaatttta ttcgttgcct
     1141 tctactttca aagctgaagc tcggtttaga gttaaaacaa aagtatcccc cgaagtcaca
     1201 aattctgaac aattcctacc ccacatttct ggagcggctg aatatatatt tttccgtgac
     1261 cgtgtaattc gtgagtttgt tgtagctcgg ttgacggtgt gcagggttct tgtttttccg
     1321 tgaccgtgga attcgtgagt ttgttgtagc tcggttgaca gtgtgcgggt tcttgtttta
     1381 actcacaatt ttttaattcg taaacaagtt ataactcggt tgcaaatgaa gatgtttgtg
     1441 ggatgtggat acctggatga caagtgaaaa ttgtgagtca aaacaagcat cgttgcaact
     1501 atatgcagac gcgatgaagt tttgactcta aaccgattct tttgtaattg caactatatg
     1561 cagacgcaac aggaaatgtt attccctgcc ttctactttc aaaactgaag ctcggtttag
     1621 agttaaaaca aaagtatccc ccgaagtcac aaattctgaa caattcctac cccacatttc
     1681 tggagcggct gaatatatat tttttccgtg accgtggaat tcgtgagttt gttgtagctc
     1741 ggttgacagt gtgcggggtt cttgtttttc cgtgaccttg gaattcgtga gtttgttgta
     1801 gtgcggttga aaatgtgcgg ggttcttgtt ttaactcaca attttttaat tcggaaacaa
     1861 gttataactc ggttgcaaat gaagatgttt gtgggatgtg gatacctgga gggtaagtga
     1921 aaattgtaag tcaaaacaag catcggattg tggtttccgt gatggttgtg gcaaaattgt
     1981 aagtcaaaac agaaaagccc ccgcaaaaaa cacgggggca tattgtctgt gattaaataa
     2041 gttactttct gtttgcaaca gttttaggta ttttcttatg gaacacatta tatcccttgg
     2101 ttgggggaat tacaggaagt tccatagagc aggccttaat ctgatagaat tctttactat
     2161 taggtgtgaa actaagagca gttccatagc tggcaggcca gtagccaaga tagtcataag
     2221 taccataagg atctgtagca gcgtaaatca gataccagtc ggttccggga acaggatccc
     2281 aagtaaggtt cacattggtt ccatcatagt caatagcaag agttggctga ggcaaagttg
     2341 cgggaattgc ttccacaacg atattatcaa tataccagta attaatttcg tacgaatctt
     2401 cacctgcagc caggaatctg atccggaaca tccggttact tgcataagct gagatatcgt
     2461 atacataggt atcccaacca taaccagcac cctcattatc aaaagaggag aaggtatcaa
     2521 atgtctgcca ggtagttcca tcccaaacct gaggtattaa caagttttcg gcatacgtgc
     2581 tgtaattatt caatgccata tcaaatttga attttacatt agaaatcccg gtagcatcta
     2641 aataccagct tgtaagatat tcctgataat tagtaatttg gggactccaa ctgaaggcag
     2701 cagatggttc ttccaaacca ttacctgagt caattatcca gttagctgca gttgtattcc
     2761 attgatttgt agcaaagtcg gcactactcc atgtctctat gaagggcaaa gagtagggaa
     2821 taggaggttc tacattaaca actacaggat cagacattgg agataatgca gtgtaataaa
     2881 caccctgcac ggtataagag taagttccgt aaggtacacc ctgatcaaga taggtagcag
     2941 tgggaaccag taaagtgttt atttgtatag cattacgata gatattataa ccccgcagga
     3001 cacggttagg aggattgctg ttgccggaag ttgtgaatcg gacagccaaa tgttccttag
     3061 aacgcaattc ctgattgatt acgggaatac tgagtaactt aggagctttt gtgcctatag
     3121 gaatatcaaa atatccctgt agattccaat tataatccaa gccatatcca gttgccatac
     3181 tttcccatgt tcccaaggaa ataagatcac cgtaaccaac tacagcagga cctgcatcac
     3241 agccagccgg ataataatca gcgccagtgg cgacaacgca ttcataaccg atccataaag
     3301 cttgggttcc gcttatagga ataggagtat taagaacaac ttcattccat tgatttacag
     3361 tgggagtatg agcttgagaa taaaccagag tggaaggggt aagcgttcca tcatttcctg
     3421 tccagacctt aatagtataa gtagtattag ctgaacgagg gaaatatttt actttactaa
     3481 gttccatatc cgcatagcca tacattgttc cggaatcaaa cctgatagcg acatcaaaag
     3541 taccacttga gccagtgcct attccatcat agtttgtacc atcatcgtaa cttaaccatc
     3601 cagggcttcc tggaatacca ctgtaatttg cccaagttag ctgaacatct tgttctgtaa
     3661 cagtggcagt aaggtttaca ggaatataaa tttgttctgc aatagcagtt ccacttagag
     3721 caatggtatg caagacctta gcggaaagat tatccaggat gttcagagta gcagtttttt
     3781 ctcctatcgt ctgaggaaca aaagataccg taaactcgta tg
//
LOCUS       DAOPFL010000120         3803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_32936_length_3803_cov_95.897545, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000120 DAOPFL010000000
VERSION     DAOPFL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32936_length_3803_cov_95.897545"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLG69_06850"
     CDS             <1..587
                     /locus_tag="PLG69_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43263.1"
                     /translation="LFIQKGNSLLEAGDFQGALVHYRKTFEIIPDYPPALEAIDRLFT
                     IQENIRKAADLYKQGEQLEATGKLNEALQAYNSAYALDPKPEYKQKANLVQNMIEANK
                     NPAAFARKIITEYKGGLLTNRIQNQKKELLKRYKEYEIQDSGWKLMLSTGQYKYEARY
                     DLLTPNETYLYVWQINLRDRSIIPLNKLSEALMQ"
     gene            584..1186
                     /locus_tag="PLG69_06855"
     CDS             584..1186
                     /locus_tag="PLG69_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43264.1"
                     /translation="MKARTKIFLFLLLCIICGTTALPLKAQSFDIKAFSDTTKYGWKN
                     YLDRNAYRQDLKQRQDLLQIYEMEAQPLNTNILKSAIIPGWGQFSTKESTKGTVILGT
                     EIVLAGTSFYFLDRALSKYKLYKQATQVDEIEKYYKDAQVPYQYSFILMGAAGIIWAY
                     NIFDVIQSTQDYNVRLWEEIVERSKSGPVYITPTGIEVRF"
     gene            1187..1612
                     /locus_tag="PLG69_06860"
     CDS             1187..1612
                     /locus_tag="PLG69_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43265.1"
                     /translation="MVKHLAKIIILIMLIILLASCSLKRSNPLDPNGNGDIVVPEPVT
                     GITHTTSSQHQNPCWVNIKWAANSASNTDGYFIYRGIGYYSSFALVDSSRTNEYVHSS
                     ANDPTVQPGDYWYRISAYKTYPEGKLEGRRSEPYWVRIP"
     gene            1615..3792
                     /locus_tag="PLG69_06865"
     CDS             1615..3792
                     /locus_tag="PLG69_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPI43266.1"
                     /translation="MDHNFYASQLNERQLEVVTDTENPVLVLAGAGSGKTRSLIYRTA
                     YLIKEKQVHPWNILIVTFTNKAARELQERLEKLLNIPVRSLWVGTFHAICCRILRFES
                     AHLPFNANFSIYDEDTQKSLLKKIYKEYGFDKQSFPINNILTRISRYKNKLMLPEDIK
                     FQGEDKDSEFATFLNIYRLYQQQLLLNQALDFDDILLWTAKLLLHNNTVREKYRAQFQ
                     YVMIDEYQDTNKAQFEIVHQIAGGHQRVCVVGDDDQAIYSFRGASLRNILEFEKDYNS
                     VHSIRLEQNYRSTGNILALANAVIANNRRRHSKELWSELPDGDKPVLAVYENEIEEAN
                     RITDAILKLQKKGIPLREIAVLYRTNAQSRVFESALIQAEIPYSIVGGVQFYQRKEIK
                     DLLAYLYVLSNLNDNESLLRIINEPARGIGQTSINRLISFANKTRISLFRAIQNVEAI
                     EELGNPAKKRISEFCLLLERWQQSVSRKPALEMVKTIVEELGLISHYQNSNDPQDIAR
                     AENLVEFVTSVSEFSEHFYQEYEREPLIADYLPYVALQTDLDRVKENMDSIRLMTLHN
                     AKGLEFEAVFIAGLEDELLPHRMCMNNQDSIEEERRLFYVGITRAKNYLHLSYTKSRR
                     LYDTFYFPVPSQFLAEVDSRLFYHSDGDFGIAQPPKRIKKTNKVITESQKYYKIGQKV
                     FHPEYGLGVILSVDGEGEDARLTISFGNRLAKIIGRYLMKNEE"
BASE COUNT         1253 a          671 c          791 g         1088 t
ORIGIN      
        1 atctctttat ccaaaaagga aacagtttac tggaagcagg tgattttcaa ggtgctttag
       61 tgcattatcg taagaccttt gaaattattc ccgattatcc tcctgccctg gaggcaatag
      121 accgtttatt tacaattcag gaaaatatcc gcaaagccgc tgatctttat aaacagggtg
      181 aacaactgga agcaacgggt aaactgaatg aggcattgca ggcatataat tctgcctatg
      241 ccttagaccc caaaccggaa tataaacaaa aggcaaattt agttcagaat atgattgagg
      301 caaacaagaa tcctgctgct tttgcccgta aaattattac tgaatacaaa ggtggattat
      361 taactaaccg tatacaaaat caaaagaagg aattgctgaa acgctataag gaatacgaaa
      421 ttcaagatag cggatggaaa ttgatgcttt ctacaggaca atataaatat gaggcacgct
      481 acgatttgct tacccctaat gagacctatt tgtatgtgtg gcaaataaat ctaagagata
      541 gaagtattat acctttaaac aagctttctg aggcattgat gcaatgaaag caagaactaa
      601 aatcttcctc ttccttttgc tgtgcattat ttgcggaaca acagctcttc ccctaaaggc
      661 acagagcttt gatattaaag cattttccga tactacaaaa tacggctgga aaaattatct
      721 ggatagaaat gcctatcgtc aggacttgaa acaaaggcag gacttattac agatttatga
      781 aatggaagct caacctttaa ataccaatat cctgaaatcc gctataattc ccggttgggg
      841 acaattttct accaaagaaa gcacaaaagg caccgttatt ttaggcacag aaatagtgct
      901 ggcaggaact tctttctatt ttctggatag agctttaagt aaatataaac tatataaaca
      961 ggcaactcag gtagatgaaa tagaaaagta ttataaggat gctcaggttc cctatcaata
     1021 ttcctttatc ctgatgggtg ctgcaggaat tatttgggct tataatatct ttgatgtaat
     1081 tcaatccacg caggattata atgtccgttt atgggaggaa attgtggaac gcagtaaaag
     1141 cggtcctgtg tatattactc ccacaggaat agaagtgagg ttttaaatgg tgaaacactt
     1201 ggcaaaaata ataatcctca ttatgctgat aattttactt gcctcctgca gtttgaaacg
     1261 cagtaatcct cttgatccta atggcaatgg agatattgta gttccggaac cggttacagg
     1321 aattactcat actacttcat cacaacatca aaatccctgc tgggtaaata taaaatgggc
     1381 agctaatagt gcatccaaca cagatggata ttttatttac aggggaattg gttattattc
     1441 ttcttttgct ttggtagata gtagtagaac caatgaatat gttcatagca gtgccaatga
     1501 ccctacagtt caacctggag attactggta tcgtatttcc gcttataaaa catatccaga
     1561 gggcaaactg gaagggcgta ggtcagaacc ctattgggtt cgtattcctt agctatggac
     1621 cataactttt atgcttctca actgaacgaa aggcagttgg aagttgttac cgatacagaa
     1681 aatccggttt tagttttagc tggagccggt tctggaaaaa cacgctccct aatttacagg
     1741 actgcttatc tcattaagga aaaacaagtg catccctgga atattttgat tgttactttc
     1801 actaataaag cagctcggga attgcaggaa cgcctggaaa aattactgaa tattccggta
     1861 aggtcattat gggtaggcac ttttcacgca atttgctgtc gcattttacg ctttgaaagt
     1921 gctcatctgc cttttaacgc caatttttcc atttacgatg aagataccca aaagtccctt
     1981 ttgaagaaga tttacaagga atatggcttt gataaacaga gctttcctat taacaatata
     2041 ttaactcgca tcagtagata taaaaacaaa ctgatgctgc cggaagatat taaatttcag
     2101 ggggaagata aagatagcga atttgccact ttcttgaata tttaccggct ttatcagcaa
     2161 caacttctgc tcaatcaggc attggatttt gatgatattt tgctgtggac agctaaactt
     2221 ttgctgcata ataacacagt gcgagaaaaa taccgcgctc aatttcaata tgtgatgatt
     2281 gatgaatatc aggataccaa taaagcgcag tttgaaatag tgcatcaaat tgccggaggc
     2341 catcaaaggg tttgcgttgt aggagatgat gatcaggcaa tttacagctt tcggggtgca
     2401 agtttacgca atattctgga atttgaaaag gattataatt cggtgcatag cattcgcctg
     2461 gaacaaaatt acagaagcac aggcaatatt ttagctttag ctaatgccgt tatagccaat
     2521 aaccgaagaa gacacagtaa agagctttgg agtgaacttc ctgatggtga taaacccgtt
     2581 cttgctgttt atgaaaacga aattgaggaa gcaaacagaa ttaccgatgc cattcttaaa
     2641 ctgcagaaaa aaggaattcc tttacgcgaa atagctgttt tatatagaac caatgcgcag
     2701 tcccgggttt ttgaaagtgc tttaattcaa gcagaaattc cttacagcat cgtgggaggc
     2761 gtgcaattct atcagcgcaa ggaaattaaa gacctgctgg catatttata tgttttaagt
     2821 aatctgaatg ataacgaatc ccttttacgc attatcaatg agccggcaag gggaattgga
     2881 caaacaagta ttaaccgtct gatcagtttt gccaataaaa cgcggatttc tcttttccgc
     2941 gctattcaaa atgtggaggc aattgaagaa ctgggaaatc ccgctaaaaa gagaatcagt
     3001 gaattttgcc tgctgctgga aagatggcag caaagcgttt cccgcaaacc tgctttggaa
     3061 atggtgaaaa caattgtgga agaattggga ttgattagcc attatcagaa tagtaatgat
     3121 cctcaggata tcgcccgagc ggaaaacctg gtggaatttg taacttccgt tagtgaattc
     3181 agtgagcatt tttatcagga atatgaaagg gaaccgttaa ttgctgacta tttgccctat
     3241 gtagctttgc aaacggattt ggatagagtg aaagaaaata tggattccat tcgcttgatg
     3301 actttgcaca atgccaaggg cttggaattt gaagcagtgt ttattgccgg tttggaagat
     3361 gaacttctcc cccacagaat gtgtatgaac aatcaggata gcattgaaga agaaagacgc
     3421 ctgttttatg taggaattac cagagcaaag aactatctgc atttaagtta tacaaaaagc
     3481 cgacgcctgt atgatacctt ctattttcct gttccaagcc aatttttggc agaagtggat
     3541 tcccgattat tttatcattc ggatggtgac tttggcattg ctcaaccacc gaaaagaata
     3601 aagaaaacga ataaagtgat caccgaaagc caaaaatatt ataaaatcgg acaaaaggtc
     3661 tttcatccgg aatacggatt aggagttatt ttaagcgtag atggtgaagg agaggatgca
     3721 cgactcacaa tatcttttgg aaatagattg gcaaaaataa taggaagata tttaatgaag
     3781 aatgaagaat gaaagagggt tga
//
LOCUS       DAOPFL010000121         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_32978_length_3799_cov_108.424145, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000121 DAOPFL010000000
VERSION     DAOPFL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32978_length_3799_cov_108.424145"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLG69_06870"
     CDS             <1..809
                     /locus_tag="PLG69_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPI43267.1"
                     /translation="FYQNKGLNVIIQPGGIKYPPYENLQLGHSDIVQMSLITAVKRDA
                     ENGNLINLAQITQRTSLMLVGKKSRGINSIPDFEGKRIGLWKTDDRLLSLIFLQQNKL
                     NMDIVDLDITINLFLKDGIDVMNVMRYNEYHQIIQAGIDPEDLFSVAFADIGLNVADN
                     GLYTTREFYTKHPKQCRDFASATIQGWFYALNNQEETVSTVLEIMHQYYLPANRPHQK
                     WMLNEICQDFTVHKNQEGILQESDFNKAKKMLTDQNIPATQQTYESFFPR"
     gene            806..2722
                     /locus_tag="PLG69_06875"
     CDS             806..2722
                     /locus_tag="PLG69_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPI43268.1"
                     /translation="MKQRQEKRGLAYQIIVFIFISLFLIFTAFIAITRILFSHIMMEN
                     AQETVSHLANETTYQIENRLTSVVNISRTILTLYRYDHFERNKLDSILHDWVYEFENL
                     EAITIAYAPSYHQEGYSRTIFHSHNRAVYQELHPADYQYLDWFQIPFELKSKYWTEPW
                     YDATATQETVISYCLPLFAEGKCVGIMRLDTKLSALQKIVSPLKLKHSGYAFLVSSIG
                     TIITHPADSLIFNESIFSLAEATNDKNLRQIGKKMINGNIDFVRLQDNAVFGDSWLYY
                     CPLLTNNWSLGIIIPHKDVVGYLNLLLIIQTLFSIIIFLTISFIVYYRTLSVSRPIRL
                     FTEVAERIGQGDFDAKLPKTGNSYEIDRLILAFEKMQESLKDYIKNLEITNAEKNRIE
                     TEVKIASEIQRKLIPENTLQPYEIKELRCYGILEPAGDIGGDLYDFFPIDKSHFLFVI
                     ADVAGKGIVASMTMTIVSTYLRTVSTYHYTASEIMQNLNNFLCKQTSASNFVTALLGI
                     INLKTGKLEFSNAGHTPMIIRKTDLSYKIFTETHSYPLGIFANREIGSMTIKLEKGEE
                     IILVTDGVTETMNATEDFLGLKGLENIIKNLSSTNPEQTVRQILQHVHSFAQNTPQKD
                     DITILVIDYKKSED"
     gene            complement(2922..>3799)
                     /gene="meaB"
                     /locus_tag="PLG69_06880"
     CDS             complement(2922..>3799)
                     /gene="meaB"
                     /locus_tag="PLG69_06880"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPI43269.1"
                     /translation="PAQELIKELLPYSGNSIRIGITGVPGAGKSTFIENFGLYLIEQG
                     HKVAVLAIDPSSTISKGSILGDKTRMELLSRNPKSFIRPSPSAGILGGVARKTRETII
                     LCESAGYDVILIETVGVGQSETTVRSMVDFFLLMQIAGAGDELQGIKRGIIELADLIV
                     INKADGDNIARTELAKRELQNVLHYIRNSTEGWQTKVVTSSALNKIGLKEIWELITEF
                     QKITKQSSVFAKRRAQQNTQWFENLIAEAVLHRFYQQEKVKELLPRLKEEVAKGKMPV
                     ALAVDTLLKNEKSEE"
BASE COUNT         1189 a          758 c          681 g         1171 t
ORIGIN      
        1 gtttttatca aaataagggc ttaaatgtaa ttatccaacc tggaggaata aaatatcctc
       61 cctacgaaaa tttgcagctg ggacatagtg atattgtgca gatgtcgtta ataacagcgg
      121 taaaaaggga tgccgaaaat ggtaatttaa taaacctggc acaaattact cagcgcactt
      181 ctttaatgct ggtggggaaa aaatcccgcg gcataaattc catacccgat tttgaaggca
      241 aaaggattgg actgtggaaa acggatgacc gcctgctatc cttgattttt ttacagcaga
      301 acaaactgaa tatggatata gtggatttgg atatcactat caatctcttc ctgaaagacg
      361 gcatagatgt tatgaatgta atgcgttata atgaatacca tcaaattata caagcgggaa
      421 ttgatccgga ggatttattt tccgttgcct ttgccgatat cggattaaat gtagctgata
      481 atggacttta taccacccgt gaattttata ctaaacatcc gaaacaatgt cgtgattttg
      541 cctctgccac aattcagggc tggttttatg ctctgaacaa tcaggaagaa actgtctcca
      601 ctgtcttgga aattatgcat caatactatc ttccagctaa tagaccccat caaaaatgga
      661 tgctgaatga aatatgccag gattttacgg tgcataaaaa tcaggaggga attctccagg
      721 aaagcgattt taataaagct aaaaaaatgc ttaccgacca aaatataccc gcaacccaac
      781 agacctatga gagctttttc ccccgatgaa acaaagacaa gaaaaacgcg gactggcata
      841 tcagataatt gttttcatct ttatctccct atttcttatt tttacagcgt ttattgccat
      901 aaccagaatt cttttttctc atataatgat ggaaaatgcc caagaaacag ttagtcacct
      961 ggcaaatgaa acaacctatc aaattgaaaa ccgcttaacc agtgttgtaa atatctcgcg
     1021 cacaatttta acactatacc gttatgacca ttttgaaaga aataaattgg attctatcct
     1081 gcacgattgg gtttatgaat ttgaaaacct ggaagcaata actattgctt atgctccttc
     1141 ctatcatcaa gagggttatt cccgcactat ttttcactct cacaaccggg ctgtttacca
     1201 ggaattgcat cctgccgatt atcaatatct ggattggttt cagatacctt ttgaactgaa
     1261 aagtaaatat tggacagaac cctggtatga tgctactgct acccaagaaa ctgttatttc
     1321 ctattgccta cctctttttg ccgaaggtaa gtgcgtagga attatgcgat tagatacaaa
     1381 gctctccgct ttgcaaaaaa ttgtttctcc cttaaagctg aaacatagcg gttatgcttt
     1441 tctggtttcc agtattggca caattattac tcaccctgcc gattctctca tttttaatga
     1501 aagcattttc agtttggcgg aagcaacaaa tgataagaat ttaaggcaaa tcggcaaaaa
     1561 aatgattaac gggaatattg attttgtgcg cttacaggat aatgccgttt ttggcgattc
     1621 ctggctgtat tattgccccc tgctaacgaa taattggtct ttaggaatca ttatacctca
     1681 caaagatgta gtgggttacc tcaatctttt gctgattatc caaactcttt tctccataat
     1741 catctttctc actatatcct tcattgttta ttatagaacc ttaagcgttt ccagacccat
     1801 ccgccttttt acggaagtag ctgaaagaat tggtcagggt gattttgatg caaaactccc
     1861 caaaacaggc aatagctatg aaatagaccg ccttattctg gcttttgaga aaatgcagga
     1921 atctctgaag gactatatta aaaatctgga aattaccaat gcggaaaaaa atcgtattga
     1981 aacagaagta aaaattgctt ccgaaattca acgcaagctg attccggaaa atacacttca
     2041 gccatacgaa ataaaagaac ttcgctgtta tggtattctg gaaccggcag gagatatcgg
     2101 tggtgattta tacgatttct ttcctataga taaatcccat tttctttttg tgatagccga
     2161 tgtggcagga aaaggaattg ttgcatcaat gactatgaca attgtctcca cctatttgag
     2221 aaccgtttct acctatcact acactgcaag tgaaataatg caaaacctga ataatttcct
     2281 ctgtaaacaa acctccgctt ccaattttgt aactgccctt ttgggaataa taaatctgaa
     2341 aaccggaaaa ctggaatttt ccaacgcagg acacacccca atgataatca gaaaaacgga
     2401 tttgtcgtat aaaatattta ccgaaaccca ttcctatccc ttaggcattt ttgccaatag
     2461 ggaaattggt tctatgacta ttaaactgga aaaaggagag gaaataattc tggtaaccga
     2521 tggtgtaacc gaaacaatga atgccacaga ggattttctg ggattgaaag gtctggaaaa
     2581 tatcattaaa aaccttagct ccaccaatcc ggaacaaacc gttaggcaaa ttttgcagca
     2641 tgtgcatagt tttgcacaaa atacacccca gaaagatgat ataactattc tggtgattga
     2701 ctacaaaaaa agtgaagatt gagaatgagg gatagaacgc agataactgg aacaaatgga
     2761 tcagcaggat tatattttta ggtaagtaaa atctgtgtta tctgtgagag atagaacgca
     2821 gatttatcag gaatacggat tttagcgcat tgatactgat tatttaatta taattattaa
     2881 gctaactaat ccagttaatc ctgttatctg tgttctatcc ttcattcttc acttttttca
     2941 tttttcaaaa gagtatccac tgctaatgct accggcattt tcccttttgc tacttcttct
     3001 ttcaaccggg gcaataattc cttcactttt tcctgttggt aaaagcgatg caaaactgcc
     3061 tccgcaatca ggttttcaaa ccactgagtg ttttgctgtg ctctgcgttt ggcaaaaaca
     3121 ctgctttgct ttgtaatttt ttgaaattca gttatcagtt cccagatttc ctttaagcca
     3181 attttattta aggcagaaga ggtaactact ttggtttgcc agccctcggt tgaatttctg
     3241 atataatgca aaacattttg cagttctctt ttggcaagtt ccgttctggc aatattatcc
     3301 ccatcagctt tattgattac tattaaatcc gccagttcaa taatccctct tttaatgcct
     3361 tgaagttcat ctcccgcacc tgcaatctgc atcagcaaaa agaaatctac cattgaacgg
     3421 acagtagttt ccgattgacc aactcctacg gtttcaatta aaattacatc ataacctgcg
     3481 gattcacaaa ggataattgt ttctcttgtt tttctggcta ctccacctaa aattccagca
     3541 ctgggagaag gacgaataaa acttttggga ttgcgggaaa gtaattccat ccttgtttta
     3601 tcgcctaaaa tgctgccttt gctgatagtg gaactggggt ctatagctaa aacagctact
     3661 ttatgaccct gctcaattaa atacaagcca aaattttcta taaaagtgct ctttcctgct
     3721 ccaggaactc ccgttatacc aatgcgaatg gaatttccgg aataaggcag aagttcttta
     3781 atcagttcct gtgcaggta
//
LOCUS       DAOPFL010000122         3789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_33089_length_3789_cov_103.550616, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000122 DAOPFL010000000
VERSION     DAOPFL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33089_length_3789_cov_103.550616"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..1079)
                     /gene="recO"
                     /locus_tag="PLG69_06885"
     CDS             complement(324..1079)
                     /gene="recO"
                     /locus_tag="PLG69_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPI43270.1"
                     /translation="MKNKLLAILIKQSQYSESSLILQFFTRQQGMVSVIAKGIRKKSE
                     KQQLLPLCEYELIAYEPKEPGLWLFSEANITRNFSVYPSSATWAAAETGLELISHLII
                     SQEDIATIYDLTISYLEYLKKVEKNAILIFWRFLNRITILSGIGNPLSYCCLCKNPLD
                     VPNAYLKTKGGFVCEKCLPEINPDNALMILSPPARNILTLLPEIAIYLEDIKINRAVV
                     NEINTVFALYWEAHHKQTLHLKGLSVLAQFYNS"
     gene            complement(1076..2479)
                     /locus_tag="PLG69_06890"
     CDS             complement(1076..2479)
                     /locus_tag="PLG69_06890"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HPI43271.1"
                     /translation="MKYSYLKQWTQEMIGYQAVIESVLLFPELLVLQTKDKKALCIVL
                     SKRDAFIFLQDNFQPPQEGKKIWQKLNNYHLTGISLAENDRLAYLELQQRDIYQQNKK
                     YFLIAELMPPQPNAILTDSELHILDALHKYSYADNPQRQILPGLVYTAPKTSFQPILE
                     EVKSPYPDGSATCNDYFINLYYNKLKKEEEVENGQKICSALKKELQKLQKKREKLQQD
                     LTNAEKADFWLACAECLKTNLHNLKTGRESFTAINYLDPALKEIEIPLQKDKSPQENL
                     QSYLKKYHKAKNGLQIITKNLAQTDAEIEKIKDLIAKAEKGELPDSGILPSKPTGHKI
                     VQNAILADKILKLKINDEFQILIGRRAKENDYLTTQLARPYDYWFHCRIYHGAHIVLK
                     CLKKTEPSPQLIELCCNLAAWFSKAKFSTNVPVDYTQIRYVRKPRKSPPGFVTYTNFK
                     SVFATPMSLKEVREKLS"
     gene            2564..3436
                     /locus_tag="PLG69_06895"
     CDS             2564..3436
                     /locus_tag="PLG69_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43272.1"
                     /translation="MKATTAIIVAVLAILTIVFAVLWINANGKSKDIMKSNEELKKLY
                     ETSTSTINEIQSSLESLEQDLSGQLFTQKEIPGTSPEERRNRIISSIANMRDQIEADK
                     KKIATLEKQLATSKSQLKGVQEIVNKLKASISEKEQIMDELQERLGILNETLETERRT
                     SAEEIQKREMTITEKEQQITQQSIEANTIYYVYGTRKELMSKGIMDRKGGLLGIGKVS
                     TVRQNIPIEQFTSMNLLETQQISFPATKKGYSILTNHPATSYKVEKEGDQNILTILDA
                     TNFRKQKFVVIELL"
     gene            complement(3511..>3789)
                     /locus_tag="PLG69_06900"
     CDS             complement(3511..>3789)
                     /locus_tag="PLG69_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPI43273.1"
                     /translation="GFGSFFILKAYVTPQINMLQACAILGAGLISGTVELISAYHQSR
                     IVATGIESIGSGHDVFAKTLILGVFPELYAIIAFAASFLIGGVIPTLA"
BASE COUNT         1203 a          646 c          736 g         1204 t
ORIGIN      
        1 gaaggaatga acttgtttgc aataagttag catagatttt ttaggagtgg agaaagcagg
       61 acataattat ccgctaacat attgatatac aaagaatagt aaatattggt actttgtttt
      121 tttggggtaa agccgatttt gacactgcat attatgttta ttatattata atatcaatat
      181 tattagtata tattattcag tttgcttttt aaaaaggaat gacaaatgta tggattcctg
      241 ttttcgcagg aataacagag gataatttca aaattgaaat ggtataacag gatttgttat
      301 tgggaggata gtgcagtttt atgttaggaa ttgtagaatt gagctaaaac ggatagtcct
      361 tttaagtgca atgtctgttt atgatgtgcc tcccaataga gtgcaaaaac ggtgttgatt
      421 tcgttaacaa cagccctatt tatttttata tcttcaaggt aaatagcaat ttcaggcaat
      481 aaagttaaga tatttcttgc tgggggtgaa agaatcatta atgcgttatc cggattgatt
      541 tctggcaaac atttctcgca aacaaagccg cctttggttt tgaggtaggc attaggaaca
      601 tcaaggggat ttttgcataa gcaacaataa cttaatggat tgccaatgcc gcttaaaatg
      661 gtaattctat ttaaaaagcg ccaaaagatg agaatagcgt ttttttccac ttttttcagg
      721 tattccagat aggaaatagt aaggtcataa attgtagcta tatcttcttg agaaataatc
      781 agatggctaa ttagttccaa acctgtttcc gcagctgccc aagttgctga actgggataa
      841 acggaaaaat tgcgggtaat atttgcctcg ctgaaaagcc ataagcccgg ttccttcggt
      901 tcataagcaa ttagttcgta ttcacaaaga ggcagaagtt gctgtttttc gctttttttg
      961 cggatacctt tggcaataac actaaccatt ccctgctggc gggtaaaaaa ttgcagaatt
     1021 aaactgcttt cgctatattg gctttgtttg atgaggattg ccagcagttt gtttttcaag
     1081 aaagtttttc ccttacttct tttaagctca tcggtgtggc aaaaacactt ttgaaattcg
     1141 tgtatgtaac aaaacctggg ggacttttac gcggttttcg gacataacga atttgagtgt
     1201 aatccacagg aacattggta gaaaattttg ccttggaaaa ccaggcagct aaattgcagc
     1261 aaagttctat taattgagga cttggctctg ttttttttaa gcatttgagg acaatatgcg
     1321 ctccgtgata aatacgacag tgaaaccaat agtcataagg tcttgccagt tgagtagtta
     1381 aataatcatt ttctttagca cggcgtccta taaggatttg aaactcatca tttatcttca
     1441 atttcaggat tttatcggca agaatagcgt tttggacaat tttgtgtccg gttggtttgg
     1501 agggcaaaat tccgctatct ggtaattcac ctttttccgc tttggcaatg aggtctttaa
     1561 ttttttcaat ttcagcatct gtctgagcca gatttttggt aattatctgt aagccatttt
     1621 tggctttatg atactttttc agatagcttt gcaaattttc ctgcgggctt ttatctttct
     1681 gtaaaggtat ttctatttcc tttaaagcgg ggtcaagata attgattgcc gtaaaacttt
     1741 cccttccggt tttaagatta tgcagattgg ttttcaaaca ttctgcacag gcgagccaaa
     1801 aatccgcttt ttctgcattt gttaggtctt gttgtaattt ttctcttttt ttctgcaatt
     1861 tttgcagttc tttttttagg gcagagcata ttttttgacc attttctacc tcttcttctt
     1921 tttttaactt attgtaatac agattgataa aataatcgtt gcaggttgcg ctaccatcag
     1981 gataagggga ttttacctct tccaaaatcg gttgaaaaga tgttttcgga gcagtgtaaa
     2041 caagtcccgg caaaatttgt ctttgaggat tatctgcata gctgtattta tgaagagcat
     2101 caagaatatg caattcggaa tcggttaaaa tggcattggg ttgaggaggc atcaattctg
     2161 cgataagaaa gtatttttta ttctgctggt agatatctct ttgctgaagc tccaaatagg
     2221 caaggcggtc attttcagct aaagaaatac ctgtaagatg ataattgtta agcttttgcc
     2281 agattttttt tccttcctga ggtggctgga aattatcctg cagaaaaatg aaggcatcgc
     2341 gtttactaag cactatgcac agggcttttt tatcttttgt ctgcaatact aaaagctctg
     2401 gaaacaataa aacactttct atcactgctt gataaccaat catttcttgt gtccattgct
     2461 taagataaga atatttcact ttagttattt tccttttgtc ttgacaaaaa aacaggttat
     2521 atttttgaat gccaaagtat aatatattac aaaggagtca agcatgaaag ctacaacagc
     2581 gattatagta gcagttctgg caattctaac tatcgtgttt gctgtccttt ggattaatgc
     2641 caacggcaaa tccaaagata ttatgaaaag caacgaagaa ttgaaaaaac tgtatgagac
     2701 atctacttcc acgatcaacg aaattcaatc aagcttggaa tcgctggaac aagacctctc
     2761 cgggcagctt tttacgcaga aagaaattcc tggaacatcc cctgaagagc ggagaaatcg
     2821 cattatatct tccatagcta atatgcgaga ccaaattgaa gcggataaaa agaaaattgc
     2881 taccctggaa aaacaacttg ccacttccaa aagccaattg aaaggtgtgc aggaaattgt
     2941 gaataaactt aaagccagta tcagcgaaaa agaacaaatt atggatgaat tacaagagcg
     3001 cttaggcatt ttgaacgaaa ccctggaaac ggaaagaaga accagtgctg aagaaattca
     3061 gaaacgcgaa atgacgatta cggaaaaaga acaacagatt actcaacaaa gcattgaagc
     3121 aaatactatt tattatgtat atggtactcg caaggaatta atgtctaaag gaattatgga
     3181 tagaaaaggc ggacttttgg gtatcggaaa ggtttctacc gtaagacaaa atattcctat
     3241 tgagcaattt acctctatga acctgctgga aacacagcaa ataagttttc cggcaacgaa
     3301 aaagggctat tctatcctca ctaatcaccc cgctacttcc tataaagtgg aaaaagaagg
     3361 cgaccagaat atccttacta ttcttgacgc aacaaacttc cgtaaacaaa aattcgtagt
     3421 aatagaactg ctgtaaccta tataaatttt aaccctttca aaaaggtgag gttaaaaaaa
     3481 cctcaccttt tttgttttgc aattttgttt ttaagctaaa gtcggaatta ctccaccaat
     3541 taggaaactg gcagcgaaag caataattgc ataaagctca ggaaacacac ccaaaataag
     3601 ggttttagca aaaacatcgt gtcccgaacc gatactttca atacctgtgg cgacaattct
     3661 tgactggtga taggcagaaa taagttccac agttcctgaa atcaaacctg ctcctaaaat
     3721 ggcacaggcc tgaagcatat taatttgggg agtaacataa gctttcagga tgaagaaaga
     3781 accaaaacc
//
LOCUS       DAOPFL010000123         3730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_33817_length_3730_cov_98.439728, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000123 DAOPFL010000000
VERSION     DAOPFL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33817_length_3730_cov_98.439728"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1321
                     /gene="eno"
                     /locus_tag="PLG69_06905"
     CDS             50..1321
                     /gene="eno"
                     /locus_tag="PLG69_06905"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPI43274.1"
                     /translation="MSDILYIIGREILDSRGNPTVEAEVHLDSEIMAIAAVPSGASTG
                     EREAIELRDGDKKRYGGKGVLKAVSNIDNISDELWGTDSLLQGQIDKIMIDMDGTPNK
                     AKLGANAMLAVSMAVARASALELNIPLYRYLGGVNAKTLPVPMSNILNGGAHADNNVD
                     IQEFMIMPLGAKSFKQALQMNAEVFHSLKAILKKRGLVTSVGDEGGFAPNLASNEEAF
                     IVIVEAIKTAGYIPGKDIYLAIDAAASSFYKDGKYLFEGKKVSSDAMIKYYESMIAKY
                     PICSIEDGLAENDWTGWKKMNAKLGSKIQIVGDDVFVTNPKIIAKGIKDNIANSVLIK
                     LNQIGTVTETIDAINMAHKAGWTTVVSHRSGETGDTFIADLAVALNTGQIKTGSISRS
                     ERIEKYNQLLRIEEELGEAAYFPGKSIIKQL"
     gene            1653..3044
                     /locus_tag="PLG69_06910"
     CDS             1653..3044
                     /locus_tag="PLG69_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPI43275.1"
                     /translation="MRNIDEKQKHSRLRISFQTSITEGIFAQIYGNLSAIGSSFIVKL
                     MVILGASPMHYSLLSAIGQVSALWQPLGVAITHKLKERKSACIKITAIGRFLTIFLGL
                     SLLFNNHLQGIWFVLILLFFSAGLQSLGANIWIAWISDLIPLSIRGRFFSKRNQILIG
                     IGLIVSYIVSFHIDLFESAKGAFKLGYIKLLRAEKFFLPQNQPWFLAGVFVFASLIGL
                     IGLFFLNRQPERPLRPVPEQRLRERYSAPFKDKNFRLLSVFGIWWMLAIGVGSPFWGP
                     FMMKKLSMNLFEIQIYNTLSVFSSLLSYQFWGRFIDKNGNKTAMQICIALGGLNPMLW
                     LFTSAGNYSILWLEALSSGFMWAGAGIITTNFILSVAEKGKEQVYSGIYGAITGLAMM
                     FSTLACGIFYPSSLTIGKRILEPEQVIFGVGGIMRWLSFIPLFFVVEKRTVPLPKALL
                     SLFREKTDKSLKK"
     gene            complement(3054..>3730)
                     /locus_tag="PLG69_06915"
     CDS             complement(3054..>3730)
                     /locus_tag="PLG69_06915"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPI43276.1"
                     /translation="KKKKSSEVIMLTYTEIRYNAREYLKGKWNNPCALIILVLYLING
                     GLSLIPYLGSVVSFLILGPFSLGLAIVFLKLVRGEEVNVELMFAGFKDFTRSLTAGLL
                     IFIYVFLWTLLLIIPGIIASYAYAMTFFIMADNPNISANEAIKASKEMMKGHKTDLFF
                     LDLSFLGWILLGILSCGIGFLWLGSYIYTANAIFYHEIRVEEASQVIIEAPYEEQTVP
                     PPDIEE"
BASE COUNT         1176 a          640 c          732 g         1182 t
ORIGIN      
        1 aaaaaacctt attcagtata tgaggattaa aaataaacag gaggaaaaaa tgtcagacat
       61 actttacatt atcggacgcg agattttaga ttcacgcgga aatccaacag tggaagcaga
      121 agttcattta gatagtgaga ttatggcaat agcagcagtt cccagtggtg catctaccgg
      181 agaacgcgaa gccatagaac ttcgtgatgg cgataaaaaa cgctatggag gcaaaggtgt
      241 cttaaaagca gttagcaata tagacaatat aagtgatgaa ttatggggaa ctgattcatt
      301 attgcagggt caaattgata aaattatgat tgatatggat ggaactccta ataaagccaa
      361 actgggtgct aatgctatgt tagctgtttc tatggctgtt gccagagctt ccgctttgga
      421 attgaatatt cctttatatc gttatctggg aggtgttaat gccaaaaccc ttcctgtacc
      481 aatgagcaat attctcaatg gtggcgccca tgcagataac aatgttgata ttcaggaatt
      541 tatgattatg cctttgggtg caaaaagttt caaacaagcg ctacaaatga atgctgaggt
      601 ttttcactcc ctgaaggcaa ttcttaaaaa acgcggttta gtaacttccg ttggagatga
      661 aggtggtttt gcacctaatc ttgcatccaa tgaagaggcc tttattgtaa ttgtagaggc
      721 aattaagaca gcaggttaca ttcccggaaa agatatttac ctggctattg atgctgctgc
      781 cagcagtttt tataaagacg gtaagtatct ttttgaagga aaaaaagtta gcagcgatgc
      841 aatgattaaa tattacgaaa gtatgattgc caaatatcct atttgctcaa ttgaagatgg
      901 cttggcagaa aatgactgga ctggctggaa aaaaatgaat gccaaattgg gcagtaaaat
      961 tcaaattgta ggtgatgatg ttttcgttac caatcccaaa attattgcta aaggcattaa
     1021 agataacatc gccaatagtg ttttgattaa attgaatcaa atcggaaccg ttaccgagac
     1081 catagatgcc ataaatatgg ctcataaagc aggttggaca accgttgtaa gtcaccgtag
     1141 cggtgaaaca ggtgatactt ttattgccga tttggctgtt gctttaaata caggtcaaat
     1201 caaaaccggt tccatttccc gcagcgaacg aattgaaaaa tataatcaac tcctacgcat
     1261 tgaagaagaa ttaggagagg cagcttattt cccgggaaag tcaataataa aacaactata
     1321 agataaaaaa accttgccgg agctgttata attgacagct ccggtttggt attgaacaag
     1381 caattatttt actattgggt atagttttcg gattgtaaag acatttagtt tatccgaaaa
     1441 caatgcagtt taggaaaaat gggttagcaa attcattctt cgccaaagag tccgcaaaac
     1501 tctgaaaaat caatgctctc aatttatggc gaatctgaat tcagcaaacc aaatacctaa
     1561 tttcactttt cactatttaa tattcctcgt agtgtaaatt ctctggacaa gaacaaccac
     1621 ctccatataa tgaataatat gagtataaaa aaatgcgcaa catagatgaa aagcagaaac
     1681 atagtcgtct tagaattagt tttcagacct ctataacaga aggtattttt gctcagattt
     1741 acggtaatct gtctgcaatc ggttcttcct ttattgtaaa gctgatggta attttgggtg
     1801 cttcaccaat gcactacagt ttattaagcg ctataggaca ggtttctgct ctttggcaac
     1861 ctttgggagt agctattact cataaactga aggaacgtaa atctgcctgt attaaaatta
     1921 ctgctatagg caggtttttg actatctttt taggtctgtc tttattgttt aataatcatt
     1981 tacagggaat ctggtttgtg cttatactgc tattttttag tgcaggttta caatccttgg
     2041 gagcaaatat ctggattgcc tggataagtg atttgattcc tctttccatt cggggacgct
     2101 ttttctccaa gcgcaatcaa attttgattg gaataggatt gatagtaagt tatatagtca
     2161 gttttcatat agaccttttt gaatccgcca aaggtgcttt caaacttggc tacataaagc
     2221 ttttgagagc agaaaaattc tttcttcctc aaaatcagcc ctggttttta gccggggttt
     2281 ttgtgtttgc ttctcttatt ggtttaattg gtttattttt tctgaaccgt caacctgaaa
     2341 gacccttgcg tcctgttccg gaacagcgtt tacgggaacg ttattcagct ccttttaagg
     2401 ataaaaactt ccgtctactt tccgtttttg gtatttggtg gatgttagct attggtgtag
     2461 gcagtccttt ttggggacct tttatgatga agaaactttc tatgaacttg tttgaaatcc
     2521 aaatctataa tactttgagt gtgttttcct cacttttatc ttatcaattc tggggtcgct
     2581 ttatagataa aaatggcaat aaaacagcta tgcagatttg tattgccttg ggaggattaa
     2641 atcctatgtt gtggcttttt accagtgccg gaaactattc cattttgtgg ctggaagcac
     2701 taagttcggg ttttatgtgg gctggagcag gaattataac taccaatttt attctttccg
     2761 tagctgaaaa agggaaagaa caggtttatt cgggaattta cggagccata accggcttgg
     2821 caatgatgtt ttcaactctt gcctgtggta ttttttatcc ttcctcactc acaattggta
     2881 aaaggatttt agaaccggaa caggtgattt tcggtgtagg tggaattatg cgttggctaa
     2941 gttttattcc tttgtttttt gtggtggaaa aaaggactgt acctttgcct aaggcattgc
     3001 tttccctttt tcgggaaaaa acggataaat cgcttaagaa ataattgaac gatttattct
     3061 tctatgtcag gaggaggaac agtttgttct tcataagggg cttcaattat aacctgagat
     3121 gcttcttcca ctctgatttc atgatagaaa attgcattgg cagtgtaaat gtagctgcct
     3181 aaccacaaaa aaccgattcc gcaagataaa attcccagta aaatccatcc caaaaaagat
     3241 agatccagaa aaaagagatc agttttgtga cctttcatca tttctttact ggctttaatt
     3301 gcttcattag ctgaaatatt gggattatcc gccattatga aaaaagtcat tgcataggca
     3361 tacgaagcaa tgattccagg aataattaaa agcaaagtcc ataaaaacac ataaatgaag
     3421 attaataatc ctgccgttaa actacgggta aaatccttga atccggcaaa catcaattct
     3481 acattaactt cttcacctct aactaattta agaaaaacta tggctaatcc caaagaaaaa
     3541 ggtcctaaaa taaggaaaga aacaaccgaa ccaagataag gaattaaaga taatccacca
     3601 ttaatcaggt ataaaacaag aattatcaac gcacagggat tgttccattt cccttttaaa
     3661 tattctctgg cattataacg aatttcagta taggttagca taatcacctc acttgatttc
     3721 ttttttttat
//
LOCUS       DAOPFL010000124         3721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_33922_length_3721_cov_80.915985, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000124 DAOPFL010000000
VERSION     DAOPFL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33922_length_3721_cov_80.915985"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /locus_tag="PLG69_06920"
     CDS             <1..1670
                     /locus_tag="PLG69_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HPI43277.1"
                     /translation="VPENLDLHRPYIDEVVLTCSCGNKMYRTPEVIDCWFDSGSMPFA
                     QWHYPFENYENFEPELFPADIISEGIDQTRGWFYSMLAISTLLKGVSSYKSCLVNELI
                     LDKNGRKMSKSHGNAVDPIELMQNYGADAIRWYLLEVSPPWVPTRFDVDGVKEILGKF
                     IGTLKNVYSFFATYANIDGFVAINYPYNWEREVEIDRWIVSRLHSLIIAVRHYFESYD
                     FTKAVRAIQSFVIDELSNWYVRRSRRRFWASAFDQDKIDAYRTLYQVLVETAKLIAPF
                     APYLAEEFFLNLEAGESVHLELYPEANKTYIDTKLEKKMQTVIDIVSLGRAARNECQI
                     KVRQPLNEMFVPTKVKDDLADMIDLVKEEVNIRNITFVSEQDGFVHYELKPDFKLLGP
                     KYGKKVQIINALLLQTPAEKVWQAFNTTGSYTLEIEGETINLLPEEVSISIIPREGYV
                     FANAKDLFVALDTTLTEELLNEGYARELINKIQFTRKEMNLDIMDNIIVKLVVPEAIA
                     KAIQVHQNYILTETLGKELVFLEKPMANMQCLDINGNKIHLGIEKAV"
     gene            1906..3693
                     /locus_tag="PLG69_06925"
     CDS             1906..3693
                     /locus_tag="PLG69_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPI43278.1"
                     /translation="MLNKDAEGIKYIVEKEGREFLLKIFYKTSLSNIENIFSLQQRLQ
                     ELNKLENKQIPKVTEIDPHFDPPYMVVEFIHGISLANLKTHNPEKLTEDFIRLVAIQI
                     VNTAISLHQHQLTLSQLALTNIMINDNDEVIILSSAITWEERDEREEIFSIAIILAQA
                     LSQHDFYKTLYRQERLSTQKFEYIYGVSVPLNKILAECLHRNILQRYRNLNDLLNALI
                     NLPPVTECEIWTEPEKALPDKLEDLSKKDIPKIRIELPFWILIALIIIVIVMLLTTNI
                     FSVLFGSKGEKLTYSSWLMPDTESDTVNNSVPVAELPREKPTQTAYGELKTGTPTPRE
                     DFRKQLPVPRITTVNPPPVAPKPRPPQNMVLIEANTLGFGRLRDNLNPNVSLSSFYID
                     KYEVTQAEWNIYMKPANCSTFGENLPVDNISWFDIAIYCNGRSEAEGLTPAYKIRGVG
                     ASRVVTCDFKANGYRLPTEAEWEMAAKAGKLYNYSGSDDPDEISWNRDNSGGKIHTPG
                     TKKPNDYGIYDMTGNVSEWVWDWYDANYIRSLPTFINPTGPETGTLKVIRGGSVANGE
                     GRNLNIMWREKGDPNRGYQYVGFRLVRSN"
BASE COUNT         1204 a          717 c          729 g         1071 t
ORIGIN      
        1 atgttccgga aaaccttgat ttacaccgtc cctatattga tgaagttgtt cttacctgtt
       61 cctgcggaaa taaaatgtat cgcactccgg aagttattga ttgctggttt gatagcggtt
      121 ctatgccttt tgctcaatgg cattatcctt ttgaaaatta tgagaatttt gaaccggaac
      181 ttttcccggc tgacatcatt tcggaaggaa tagaccaaac tcgcggttgg ttttattcaa
      241 tgcttgccat ttctactttg ctaaaaggcg tttccagtta taaaagttgt ttggtaaatg
      301 aactgattct ggataaaaat ggcagaaaaa tgagtaaatc acacggaaat gcagttgacc
      361 ctattgaact tatgcaaaat tatggagcgg atgcaattcg ctggtatctt ttagaggtct
      421 cccccccttg ggttccgacg cgttttgatg tagatggcgt taaagaaatt ttaggcaagt
      481 tcattggtac tttaaaaaat gtttattctt tcttcgccac ctatgccaat attgatggtt
      541 ttgtggcaat taactatcct tataattggg agcgggaagt tgaaatagac cgttggattg
      601 tttcccgttt gcattcactt attattgctg ttcgccacta ttttgaaagt tatgatttta
      661 ccaaagcggt tagagctatt caaagttttg ttattgacga actttccaac tggtatgtgc
      721 gaagaagtag acgccgtttt tgggcttcag catttgacca ggacaaaata gatgcctata
      781 gaaccctcta tcaagttctt gtagaaaccg ctaaacttat tgctcctttt gctccctatc
      841 tggcagaaga gttcttctta aatctggaag caggtgaaag tgtccatctg gaattgtatc
      901 ctgaagcaaa taagacctat atagatacca aactggaaaa gaaaatgcag accgttatag
      961 atattgtttc tttaggtaga gctgccagaa atgaatgcca gataaaagtg cgtcaaccct
     1021 taaacgaaat gtttgttccc accaaagtga aagatgattt ggcagatatg atagacctcg
     1081 taaaagaaga agttaatatc cgtaatatca cttttgtaag cgaacaggat ggctttgtgc
     1141 attacgaatt gaagcccgat ttcaaactgc tgggtcctaa atacggtaaa aaagtacaaa
     1201 ttattaacgc tcttttgctt caaaccccgg cagaaaaggt ttggcaggca tttaatacga
     1261 ccggttctta taccttagaa attgaaggcg aaactatcaa tctattgcca gaagaagtca
     1321 gcataagcat cattccccgt gagggatatg tttttgctaa tgcgaaagac ctctttgtag
     1381 ctttagatac tactttaacg gaagaactct taaacgaggg ctatgcgcgt gaactgatca
     1441 ataaaatcca atttacccgc aaagaaatga atttggacat tatggataat attattgtaa
     1501 agctggtagt cccggaagca atcgccaaag caattcaggt gcaccagaat tatatcttaa
     1561 cagaaactct tggaaaagaa ctggtttttc tggaaaaacc aatggcaaat atgcaatgtc
     1621 tggatatcaa tggcaacaaa attcacttgg gtattgaaaa agctgtctaa taagggttag
     1681 gaggcaatca ttaataaata ttgtatcaat tgtggaaacc ctatcccgga gaaggcaaaa
     1741 ttctgccccg tctgcggaga aaagcaaatc catgaacagg ataagcccca gctaacagaa
     1801 accaaaaaag aaaaagcaca aaaatctgca gaagcaattt cccacagaaa ttctcattta
     1861 cttaagaatc ctggtagcac cttcagggat tataccataa ttagaatgct gaataaagac
     1921 gctgagggca taaaatacat tgtggaaaag gaaggcaggg aatttctgct gaaaatattc
     1981 tataaaacaa gtttaagcaa catagaaaat attttttctc tccagcaacg cctccaagaa
     2041 ctaaataaac tggaaaataa acaaattcct aaagttactg aaattgatcc gcattttgat
     2101 cctccttata tggtagtgga atttattcat ggtatttctc tggcaaacct taaaacccat
     2161 aatccggaaa aattgactga ggactttatc cgcttagtag cgatccaaat tgtaaataca
     2221 gcgattagtt tacatcaaca tcaacttacg ctcagtcaac tggctttaac caatataatg
     2281 ataaatgata atgatgaggt gataattctt tcttccgcta tcacctggga ggaaagagat
     2341 gaacgggaag aaatattttc tattgccatt attttagctc aggcattatc ccaacacgat
     2401 ttctataaaa cattataccg tcaggagcgc ttatccaccc aaaaatttga atatatatac
     2461 ggagtaagcg ttcccctcaa taaaatcctt gccgaatgtt tgcaccgcaa tattttacag
     2521 cgttatcgta acctaaatga tttactgaat gccttaatca atctgcctcc tgttacggaa
     2581 tgtgagattt ggacagagcc agaaaaggca ctaccggata aattggaaga ccttagcaaa
     2641 aaagatattc ctaaaatcag aattgaactc cctttctgga tactgattgc acttatcatc
     2701 atagttattg taatgttgct aactaccaat attttcagtg ttctctttgg cagcaaggga
     2761 gaaaaattaa cttattcctc ctggcttatg cctgatacgg aatcggacac tgtaaataat
     2821 tctgttcccg ttgctgaatt acctcgcgaa aaaccaaccc aaacagctta tggtgaattg
     2881 aaaaccggta ctcccacccc cagagaggat tttcgtaagc agctccctgt tcccagaatc
     2941 acaactgtta atcctccacc tgtagctccc aaaccccgtc ctccccaaaa tatggttctt
     3001 attgaagcaa atactttagg ttttggacgc ctgcgggata atctcaatcc taatgtatcc
     3061 ttatcctctt tttatattga caaatatgaa gttactcagg cagaatggaa tatttatatg
     3121 aaacccgcaa attgttccac atttggagaa aatttacctg tagataacat cagctggttt
     3181 gatattgcta tttattgcaa tggcagaagt gaagctgagg gcttaacccc tgcatataaa
     3241 attagaggag tcggggcttc cagagtcgta acttgtgatt ttaaagcgaa cggctaccgt
     3301 ctccctactg aagcagaatg ggaaatggca gctaaagcag gaaaactata taattacagc
     3361 ggctcagatg accctgatga aatctcctgg aatagagata acagcggcgg caaaattcat
     3421 accccaggaa ctaaaaaacc aaacgattat ggtatttacg atatgaccgg aaatgtatca
     3481 gaatgggttt gggattggta tgatgcaaat tatatccgtt ccctgcctac ttttatcaat
     3541 cctacaggtc ccgaaacagg aaccctgaaa gttatccgcg gtggaagtgt tgcgaatggt
     3601 gaaggtagaa atctaaatat tatgtggaga gaaaaaggag accccaatcg cggatatcaa
     3661 tatgtcggct ttcgcttagt gcgcagcaac taatatttct tttgcaaaga gaagaaaaaa
     3721 g
//
LOCUS       DAOPFL010000125         3695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_34253_length_3695_cov_105.413736, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000125 DAOPFL010000000
VERSION     DAOPFL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34253_length_3695_cov_105.413736"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..79
                     /locus_tag="PLG69_06930"
     tRNA            2..79
                     /locus_tag="PLG69_06930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Pro,seq:tgg)
     gene            99..174
                     /locus_tag="PLG69_06935"
     tRNA            99..174
                     /locus_tag="PLG69_06935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Val,seq:tac)
     gene            223..1194
                     /locus_tag="PLG69_06940"
     CDS             223..1194
                     /locus_tag="PLG69_06940"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPI43279.1"
                     /translation="MKIAITGANGFVGSNLVKHFLSAGYEVKAIIRANATTTFIPSSA
                     QIVRVDYDDVTSLLSAFTGIDIIIHNAGKTKANNAEQMFKANLGITEKIIAAGNRIAE
                     SQQIIYLSSQAASRPSINNIPVKEDELGAPITIYGKSKLAAEEVIKQKCKKYYTIIRP
                     CSVYGCGDKDFLQLFKIVDRGFSFQIGHKDKLLNMIYVSELCRFIELCVNNPSAYSQT
                     FFATDGQIYKQSDIFSAIAKALNKHPITITVPESLAKLVFYAGDLYGHLFHKEVVVNK
                     EKMKEILADAWLADISKAQNILGWQPITNLENNIKDTAKCYRALGWL"
     gene            1236..2855
                     /locus_tag="PLG69_06945"
     CDS             1236..2855
                     /locus_tag="PLG69_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43280.1"
                     /translation="MLEENISETDTISYTVHEIAKTDIENWIKKNRIDIRESVFSTAP
                     IHLDLGDLYHNCYFYLGRTNLPMPERNGFETFSGIFSPALYHGSFNTLYPVKKYQRTV
                     NYTNRTYPFAPVLTSVDAGLGDYDHHYARINFVKNELFSYSELEYQGDLLAQNGFWSD
                     ITSAETAMKHRLSRKGKLFNWEAEYASWKKDIAMNYLLPFYWQLTNFKIDYELQQIYA
                     ELGIPWVKLKFLQESETASAREFYETYKRKSTRVQINTEQKFGFWQYQTLYEHSWNKD
                     DKYFCGFYDEEKYEDKLALAFKIDNPLEIDLSGDWLDWKRGRLFADISYSLQNFEAGV
                     TYKQLLGKDPSSLKVKDIFNSSGELELIDISIPREVSVQFSYNLWNIKPLIAVGSKEI
                     KQKADSTFFNLEKQQPFLRLAMDANFKWKNWQLRARPEWVISKYYQNLTESPEYRFQS
                     VQQIKYHLPYDNSVVAGCGIYGHSGYYIANAVNPSLIEASTILDAWAGFDVSNYFELR
                     AGIKNALSSAIYGAYSVPTSLYVQVLWLYLN"
     gene            3003..3131
                     /locus_tag="PLG69_06950"
     CDS             3003..3131
                     /locus_tag="PLG69_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43281.1"
                     /translation="MAHHSKNDLIPYFGALYDFSVLGVKIIGAVDVLDKNTLFILN"
     gene            3166..>3695
                     /locus_tag="PLG69_06955"
     CDS             3166..>3695
                     /locus_tag="PLG69_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPI43282.1"
                     /translation="MKLFETHAHLDHPDFDTDRETIINKCFSSGIEYIINVAFNKETA
                     YSSLELAKKHLHIFSTVGFHPHDAVDFDAEVVKKLAREKKVLAIGEIGLDFFRNLSPF
                     TVQREVFNNQAHLAVEYDLPIIVHDRDAHQECYNILKKNKVKEAVFHCFTGDIIFAQQ
                     VLDEGWMISFTANITYA"
BASE COUNT         1187 a          648 c          733 g         1127 t
ORIGIN      
        1 acggggtgta gcgcagcccg gttagcgcac ctgctttggg agcagggagt cggaggttcg
       61 aatcctctca ccccgaccaa tgtaacatat tgtggcttgg gcgcttagct cagttgggag
      121 agcacatgct ttacacgcat gtggtcgggg gttcgagtcc cttagcgccc accattttta
      181 ttttatagtt tggaaataga taaatacaat aagtggtgag gaatgaaaat agcaatcaca
      241 ggagccaacg gatttgtagg cagtaatctg gttaaacact ttctttctgc tggctatgaa
      301 gttaaggcaa taatccgggc taatgctacc actacattta ttccttccag tgcacaaatc
      361 gtaagggtag attatgatga tgtaacttca ctcctttccg cttttaccgg tatagatata
      421 ataatccata atgccggaaa aacaaaagca aataatgcgg aacagatgtt caaagccaat
      481 ttgggtatta cggaaaaaat tattgcagca ggcaatagaa ttgccgaatc acagcagatt
      541 atttatctta gttcgcaggc agcatcgcgt cccagtatta ataatattcc cgtaaaagaa
      601 gatgaattag gagcaccaat aacaatttat ggtaaaagca aactggctgc agaagaggta
      661 attaagcaaa aatgtaaaaa gtattatacc attatccgtc cctgttctgt ttacggctgt
      721 ggagataagg actttttaca gctttttaaa atagtggata gaggatttag tttccaaata
      781 gggcacaaag ataaactact gaatatgatt tatgtttccg aattatgtcg gtttattgaa
      841 ctctgtgtaa ataatccctc tgcctattct cagacctttt ttgctaccga tggacagatt
      901 tacaagcaga gcgatatttt ttccgctatt gctaaagccc tgaataaaca tcctataaca
      961 attactgttc ccgaaagttt agccaaactt gtattttatg cgggagacct gtatgggcat
     1021 cttttccaca aagaagtggt agttaataag gaaaaaatga aggaaattct ggctgatgcc
     1081 tggctcgcag atatctctaa agctcaaaat attcttggct ggcaacccat aaccaatctg
     1141 gaaaataata ttaaggatac cgcaaagtgt taccgcgcat tgggttggct ataatatttg
     1201 ttctgctgtt cctacctttg agcgcggaag aaatattgtt agaggaaaat atatctgaaa
     1261 ccgatacaat aagttacacc gtccacgaaa tagctaaaac agatattgag aattggataa
     1321 aaaagaacag aattgatatt cgtgaatctg ttttcagcac tgctcctatt catttagacc
     1381 tgggtgattt gtatcataat tgttatttct atttgggaag aaccaattta ccgatgccgg
     1441 aaagaaacgg ttttgagacc ttttccggga tattttcacc tgctttgtat cacggttcat
     1501 tcaatacact atatccggtt aaaaaatatc aaagaacggt aaattatacc aatagaactt
     1561 atccttttgc tcctgttcta acttctgtgg atgcaggact gggtgattat gatcatcact
     1621 acgccagaat taattttgtt aaaaacgagc tgttttctta ttcagaattg gaataccaag
     1681 gtgatttact agctcaaaac ggtttctggt cggatataac ctctgcagaa acagcaatga
     1741 aacatcgtct ttcccggaaa ggaaaactat ttaactggga agctgaatat gcaagttgga
     1801 aaaaagatat tgcaatgaat tatttgctcc ctttttattg gcaacttacc aacttcaaaa
     1861 ttgattatga attacagcag atttatgcgg aattgggaat cccctgggta aaactgaaat
     1921 ttttacaaga atccgaaact gcttctgcca gagaatttta tgaaacatac aaacgtaagt
     1981 ccacaagagt tcagattaac acggaacaga aattcggttt ttggcaatat caaactttgt
     2041 atgaacatag ctggaataaa gacgataaat acttctgcgg attctatgat gaagaaaaat
     2101 atgaagataa acttgccctc gcttttaaga tagataaccc gctggaaatt gacttaagcg
     2161 gtgactggct tgactggaaa cggggaagat tatttgccga tatttcatat tctcttcaaa
     2221 actttgaagc gggagtaacc tataaacagc ttttaggcaa agacccttct tccctgaaag
     2281 ttaaagatat ttttaattcc tcgggagaat tggagctgat agatatatcc attccccgtg
     2341 aggtatccgt tcaattcagc tataaccttt ggaatattaa acccttaatc gctgtgggca
     2401 gcaaagaaat taaacaaaaa gcagacagca ctttttttaa tttggaaaaa caacaaccct
     2461 tcttgcgcct agcaatggat gctaatttca aatggaaaaa ttggcagttg agagccagac
     2521 ctgaatgggt aataagcaaa tactatcaaa acttgaccga aagtcccgaa taccgatttc
     2581 aaagtgtgca acaaataaaa taccatttac cttacgataa ttcggtagtt gccggttgcg
     2641 gtatctatgg ccactccgga tattatattg ccaatgccgt aaacccatct ttaattgagg
     2701 catcaacaat tttggatgcc tgggctggtt ttgatgtaag caactacttt gaattacgtg
     2761 cgggaattaa aaacgctctt tcttcagcaa tttatggtgc ctattcggtt ccgacatcac
     2821 tctatgtgca agttctttgg ctctatttga attaacgaaa aaacaaaaag tgctggagta
     2881 ttaaagtact ggagtgctgg agtgttggag tgctgaagtt cgtaagttat ataaatatca
     2941 aattgaaaat gcataaatat tattttacct tatgtagatt cctgcttcca caggaataat
     3001 aaatggctca tcattccaaa aatgatctta taccttattt cggtgctctt tatgatttca
     3061 gtgtcctcgg tgtaaaaata atcggtgctg ttgatgtgct tgacaaaaat acgctgttta
     3121 ttttgaattg aatttatata ataaatgagg tttaataatt gaaaaatgaa attatttgaa
     3181 acacatgccc atctggatca tcccgatttt gataccgaca gagaaaccat aattaacaaa
     3241 tgctttagca gtggcattga atatatcatt aatgttgctt tcaataaaga aacagcatat
     3301 agttcattgg agctggctaa aaagcatttg cacatctttt ctactgttgg ttttcatcct
     3361 catgatgcag tggattttga tgccgaggtg gtaaaaaaac tagcaagaga aaaaaaggtc
     3421 ttggctatcg gggaaatcgg actggacttt tttcggaatt tatctccttt tacggttcaa
     3481 agagaggttt ttaataatca ggcacattta gctgtagaat atgatttgcc aataattgtt
     3541 cacgacagag atgcccatca ggaatgttat aacattctga aaaaaaataa agttaaagaa
     3601 gctgtctttc attgttttac gggtgatatt atttttgcac aacaggtttt ggatgagggt
     3661 tggatgattt cttttacggc taatatcact tatgc
//
LOCUS       DAOPFL010000126         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_34581_length_3670_cov_78.078008, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000126 DAOPFL010000000
VERSION     DAOPFL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34581_length_3670_cov_78.078008"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1034)
                     /locus_tag="PLG69_06960"
     CDS             complement(102..1034)
                     /locus_tag="PLG69_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPI43283.1"
                     /translation="MIHIFKVSEQRFVPALTMDEAFWIHLENPSPEETGKIVKKYNLE
                     EDFITDLQDADENARLEWDEGALLTILRVPVYYKHRSAKISFATAPMGIISTEDKLIT
                     VAFYDNEILTQYLEGKHRPFNITQQSFLLSISLRTAIYFLKFLKEINRRTTIIEDELQ
                     QSMKNKELIRLVRMEKSLVFFNTSLKSNEIILERLQRTRWLLNDPDAEDMIEDVIIEN
                     KQAIEMANIYSSILSGMMDAFASIISNNLNVVMKFLTSITIIISVPTLIVSIYGMNVP
                     LPFQESNFAFALIMGISVLISLILVLVFIHKKYF"
     gene            1378..>3670
                     /locus_tag="PLG69_06965"
     CDS             1378..>3670
                     /locus_tag="PLG69_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPI43284.1"
                     /translation="MKRLSLLLVMIFILGLMVPMLNASEVTIGAGNETGRIPVDMYYK
                     NSLFECLYYQDELLIVNGSITGVAFYNNFSSNLPGMPINIWLGITTQTDLSGGWIPST
                     QLTQVFTGNVDFPSGTNTINITFTTPFQYSGGVLVMMVERVMDSTWHSSSDLFACQTI
                     GTNRALNIYSDSIDYDPANPPTGTAASGKFPKTTFFYTGQGIGNDLACLSITGNTTPS
                     VGQSYQYVVTVKNNGQNAQNTYTVKLMQTGDVELASLPGLPINEAQTLTYTFNWTPSV
                     AGPTTLYGKVILATDEIPSNNQSPALSIAVQPAGIQAVTIADGTATMRIPMDFYYKNS
                     LSETIYLSDELGFVSGTITSLAFYNNFYDSPANGATKIWLGSTNVQDLSGGWIPSTQM
                     TLVFDGNITYPSGINTITIPLQTPYMHTPGNLVMMVQRPMDTQYYSSSDNFYGLTIGT
                     NRTLKVQSDGTAYDPAAPPAGTTPVGQVPKTTIFYTGQQIVNDLACLTITGSATPSVN
                     SPTNYAITVKNNGTATQTNYTVKLMKEGGVLITSVPGTSIASLQTATFNLSWTPTAVG
                     TTYLYGVVELAGDEIATNNQTGNLTVQVMEAGLTVVQIGQGTATNSTTGTPTPYGTFY
                     KNFRQQYLYKADDIYAAGGAPGLITALAFNVTSVNNCSPMPNYTIKVKHTDQTQLTTT
                     FEIGEYTTVWNSASYLPTAGWNIHTFTTPFFWNGASNLLIDICTDLITGDWTENASVP
                     YTPTSYPSCLRFQNDTQPASAATT"
BASE COUNT         1093 a          784 c          675 g         1118 t
ORIGIN      
        1 gcagtttact gcaagcacct tcataaccac gcaataaatg agctgcggaa aaggtttcaa
       61 ttacatttaa gaaaaacaat gttaactcct tttttaagtg tctaaaagta tttcttgtga
      121 atgaaaacca gcaccaaaat aagggaaatt aaaacagaaa ttcccattat tagagcaaag
      181 gcaaagttgc tttcttgaaa aggcaaagga acattcattc catagatgct tacaataaga
      241 gtcggcactg aaatgatgat tgttatagaa gttaagaact tcattactac atttaaattg
      301 ttggaaatga tagaagcaaa agcatccatc attccgctta aaatgctgct gtaaatattt
      361 gccatctcta ttgcctgctt gttttcaatg attacatcct caatcatatc ctcagcatcg
      421 ggatcgttca ataaccagcg ggttctttgc aaacgttcca aaataatctc gttggatttt
      481 aaagaagtgt tgaagaaaac cagggatttt tccatacgca ccagacggat aagttcttta
      541 ttcttcattg attgttgtaa ctcatcttca ataatagtag tgcgacggtt gatttctttt
      601 aagaacttta aaaaatagat cgccgtccgc agacttatac ttaataaaaa actctgctga
      661 gtgatattaa aaggacgatg cttgccttcc agatattggg tgaggatttc attatcataa
      721 aaggcaacag taatcaactt atcttctgtg gaaattatcc ccatcggtgc tgtagcaaaa
      781 gagatcttgg cagagcgatg cttataataa acaggcactc gtaaaattgt taacaacgct
      841 ccctcatccc attccaaacg ggcattttca tctgcatcct gaaggtcggt tataaaatcc
      901 tcttccagat tgtatttctt aactattttc ccggtttctt ccggagaggg gttctccaga
      961 tgtatccaaa atgcttcatc catagttaac gccggaacaa aacgctgctc gcttactttg
     1021 aaaatgtgga tcataataac ctcctttccg tatctatttt cttctgccat ttttttttac
     1081 cttgtttttg tggcaagcaa aaagtccaag ttcggattgt ttatagggtc taaaactcca
     1141 gaccctgaaa aaacttgctt gacagaattc ttgaattaat ttatcagtgt attcaatgag
     1201 aattatatat tcttatgtat atagtgtgta gtttcctgct attcttagaa ggcgggatga
     1261 taatgtccaa agtaaaatta aagaaacagt ttccgagggc agtatgagtt ctgtaatttc
     1321 ggaactgaac aaaaaaataa ccccaaaaat aataaaaaaa tgggaaagga gataactatg
     1381 aaacgactat cattactcct cgtgatgata ttcattctgg gcttaatggt tcctatgctg
     1441 aatgccagcg aagtaaccat tggcgcagga aacgaaacag gtcgtatccc ggtagatatg
     1501 tattataaaa actcgctttt tgaatgctta tactaccagg acgagctgtt aattgtgaat
     1561 ggaagtatta ctggagttgc tttttataac aatttctcct ctaatcttcc aggtatgcct
     1621 attaatattt ggctgggtat tactacccaa acagatttga gcggtggctg gataccttcc
     1681 actcaactaa ctcaggtttt cacaggcaat gtggatttcc cttctggaac taacactatt
     1741 aatatcactt tcactacccc cttccaatat agtggtggtg ttttagtgat gatggttgaa
     1801 cgggtaatgg atagcacctg gcattcctca tctgatctat ttgcctgtca gaccatcggg
     1861 acaaaccgcg cgttgaatat ctatagtgac agtattgact atgatcctgc caatcctcca
     1921 actggaacag ccgcaagcgg taaattcccc aaaaccactt tcttctacac tggtcaaggt
     1981 atcggtaatg acttagcttg tttaagcatt accggaaata ctactcccag tgttggccaa
     2041 tcttatcagt atgttgtaac cgttaaaaac aacggtcaga atgcacaaaa cacttacact
     2101 gttaaactga tgcaaacagg tgatgtagaa cttgcttctc ttcccggctt gcctattaat
     2161 gaagctcaaa cccttactta cacttttaac tggaccccca gtgttgcagg tcccactact
     2221 ctttatggaa aagttatttt agcaactgat gaaatacctt ccaacaacca aagtcctgct
     2281 ttgagcattg ctgttcaacc ggcaggtatt caagcagtta caattgcaga tggcacagcg
     2341 acaatgcgga ttccgatgga tttctactat aagaattcct tatctgaaac tatctactta
     2401 tctgacgaat taggttttgt aagcggaact attacttctt tggcttttta caataacttc
     2461 tatgattccc ctgccaatgg agccactaag atttggttgg gttcaactaa tgtgcaagat
     2521 ttgagtggtg gttggattcc ttccactcaa atgactttgg tgtttgatgg caacatcact
     2581 tatccttccg gaataaacac cattaccatt cctttgcaaa caccctatat gcatactccc
     2641 ggaaacttgg taatgatggt tcaacgtcca atggataccc aatattattc atcttctgat
     2701 aatttttatg gcttaaccat tggcaccaac cgcactttaa aagttcaaag tgatggaacc
     2761 gcttacgatc ctgctgctcc tcctgcggga actactcctg tggggcaggt tcccaaaact
     2821 acaatctttt atactgggca gcagattgtg aatgatttgg cttgtcttac tattaccgga
     2881 agtgcaactc catcagtaaa ttctcccaca aactatgcaa ttacggttaa aaataatgga
     2941 actgccactc aaaccaacta cactgtaaaa ctgatgaaag aaggtggcgt gctaattacc
     3001 agcgttcccg gaacctcaat tgcttcgttg cagaccgcta ctttcaacct tagttggact
     3061 cctactgctg ttggaactac ctatttatat ggtgtagttg aacttgcggg tgacgaaatt
     3121 gctaccaata accaaacagg aaaccttact gtgcaagtaa tggaagcagg acttactgta
     3181 gtgcaaatcg gtcagggaac tgctacaaat agcactaccg gtactcccac accttatgga
     3241 actttctata agaacttccg tcagcaatat ttatacaaag ctgatgatat ctacgcagct
     3301 ggtggtgcac ctggtttgat tactgctttg gcattcaatg ttacttcagt gaataactgt
     3361 tctcctatgc cgaactatac tattaaagta aagcatacag accagaccca actgaccact
     3421 acttttgaga taggggaata tactactgtt tggaatagtg ccagttactt acctacagcc
     3481 ggctggaata ttcatacttt cactactccc ttcttctgga atggtgcttc caatctctta
     3541 attgatatct gcacggattt gattaccggt gactggacgg aaaatgcttc tgttccttac
     3601 actcctacta gttaccccag ctgcttacgg ttccagaatg atacacaacc tgcttccgcg
     3661 gcaactaccg
//
LOCUS       DAOPFL010000127         3627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_35212_length_3627_cov_94.978163, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000127 DAOPFL010000000
VERSION     DAOPFL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35212_length_3627_cov_94.978163"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /gene="rnpB"
                     /locus_tag="PLG69_06970"
     ncRNA           <1..166
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG69_06970"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            240..1091
                     /locus_tag="PLG69_06975"
     CDS             240..1091
                     /locus_tag="PLG69_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43285.1"
                     /translation="MLKKLIIFLILATAIALFALANRPPKEFVLQTDVLNEASGIDFG
                     ILNKQVLFSHNDSGNKAEIYALTIDGKILSTLILKGTKNRDWEDIAVAKDPQNDISYI
                     YIADIGDNNAKYNTVYVYRFKEPQLTGKNEILQISAVDTIEIIYEDGPRDAEAIFLEP
                     KTGDIYIISKREEQVGLYRVPYPFNFKDKNNARRIASLPLSWVTAADLSNNGKLLLVK
                     TYTGIYRYKIMLDDKNIIILSKEAKTMPYITEPQGEAVCWDAKNKGYFTLSEAAAGIN
                     QTLYYYK"
     gene            complement(1133..1654)
                     /locus_tag="PLG69_06980"
     CDS             complement(1133..1654)
                     /locus_tag="PLG69_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPI43286.1"
                     /translation="MRKLNSANYKDVNFCLRCGHKLELKTDHENKLRPHCPACNWVYY
                     KNPIPAVAVVLFNENRELLLVKRGLQPKAGFWALPSGYMEINLTPEENALQELEEETG
                     LKGKIMHCVGWFFGKSPIYERVLSIGFRMKAIGGKLQAGDDAVDVKFFPLNNLPVIAF
                     DAHRDFIAKETKL"
     gene            complement(1842..3302)
                     /locus_tag="PLG69_06985"
     CDS             complement(1842..3302)
                     /locus_tag="PLG69_06985"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPI43287.1"
                     /translation="MMKMWKQIVLVIVALFLMSCAIAKDNMIDPNAPNPVVEVVKNVR
                     DAVVQIRVEAKVTVQNYINPFFNDPWFRQFFNFPQEQTRPIVSLGSGFIYEYNPNTRE
                     AFILTNNHVVEKGREGTITVTMADKVVYTATVVGLDPNTDVAVIKIVVKEGEKVTVAP
                     LGDSDKLQIGEWAIAIGNPFSEGLDRTVTLGVISATSRANLNLGDNAPIYQDFIQTDA
                     AINSGNSGGPLLNIKGEVIGINAALASTNGGNVGIGFAIPINLAKRVVEDLVASGKVT
                     RAYIGILAQEITQDIMESYGLKEVAGVLVAKVEKDSPAEKAGLQVEDIILEINGQKIT
                     SVPKFRIAIATAKAGQEVALKILRNKKEMTVKVILEAYPEDTIATADTTKGTLATGIT
                     VEAIDSPTAKRLNVKSDKGVVVTKVEPNSVAAKSGLKVGYVILKIEGKEVNSPKEFNT
                     ELEKAKTNMEKENRKTIRLYVLDTNNQPSILILKFE"
BASE COUNT         1088 a          772 c          635 g         1132 t
ORIGIN      
        1 caggaatgcc ttatccggtg caatgccaaa taggaaagct atgatgttgc ccgcatcgct
       61 ttcgggtagg cagctggagc tgttttgtga aaaacagcca agatggatga acatcacttt
      121 cgggaaactg aaagcacaga atccggctta taaatcttcc cctttttttc ggaagaacga
      181 cattcttgtc gttcacatac aaaatggttg atgaaaacat caacctttta gaggtataaa
      241 tgctcaaaaa gctgataatc tttctcattc tggcaactgc tatcgctctt tttgctttag
      301 caaatagacc acccaaagaa tttgtgttac aaaccgatgt tctgaacgaa gcatcaggca
      361 ttgattttgg catacttaat aaacaggtgc tttttagcca taacgattca ggaaataaag
      421 ctgaaattta tgctctcacc atagatggca aaattctttc taccctaatt ttaaaaggca
      481 ctaaaaaccg tgattgggaa gatattgccg ttgccaaaga cccccaaaat gacatcagct
      541 atatttatat tgccgatatt ggtgataata acgctaaata taatactgtt tatgtttacc
      601 gctttaaaga gccacagctt acaggcaaaa atgaaatcct gcaaattagc gcagttgata
      661 ctatagaaat aatttacgaa gatggacctc gcgatgcgga agcaatcttt ctggaaccca
      721 aaacaggaga tatttatatc atcagtaaaa gagaggaaca ggtaggactt taccgcgttc
      781 cctatccttt caatttcaag gataaaaaca atgccaggcg aattgcttct ttacctcttt
      841 cctgggtcac agctgcagac ctgagtaata atggaaaact gctgctggtt aaaacctata
      901 cgggcattta cagatacaaa ataatgctgg atgataaaaa catcattatc ctcagcaaag
      961 aagccaaaac tatgccctat attactgaac ctcaaggtga agcagtttgt tgggatgcca
     1021 aaaacaaagg ttatttcacc ctttccgaag ctgctgcagg aataaaccaa accctctatt
     1081 attacaaata gtgcaatcct cttttaacct cttgacttct cgacctcttg acttatagct
     1141 ttgtttcttt ggcaataaaa tccctgtggg catcaaaggc aataacaggc agattattca
     1201 agggaaagaa ttttacatca acagcatcat ctccggcttg aagttttccc ccaatagctt
     1261 tcatcctgaa tccgatactg agaactcttt catagattgg acttttgcca aaaaaccaac
     1321 caacgcaatg cataattttg cctttaagac ctgtctcttc ctccagttcc tgaagggcat
     1381 tttcttccgg agtgaggtta atttccatat aaccactggg caaagcccaa aaacctgctt
     1441 tgggttgcag acctcgtttc actaaaagta attccctgtt ttcattgaag agcacaactg
     1501 ctactgctgg aataggattt ttgtaataaa cccaattaca agcaggacaa tgaggtctca
     1561 acttattttc gtggtctgtt ttcagctcaa gtttatgccc gcaccggaga cagaaattta
     1621 catctttgta gttagcagaa ttaagttttc tcatattttc tttttaattt ctccaatttc
     1681 ttttgtcaag cagaaaaagt tatatagtat aaaactctct ggattcctgc ctgtacagga
     1741 atgacaaaga aatttcccaa acggatatga tattagccgg ggaagtgaaa aagcggaaga
     1801 caatattatc ttccgcttta atctgtttct ctctgtttag cttattcaaa ttttagaata
     1861 agaatggaag gttgattgtt agtatccagc acatacaagc gaatggtctt cctgttttcc
     1921 ttttccatat tggtcttcgc tttttccagt tctgtattga attctttggg gctgttcact
     1981 tctttgcctt caatttttag aatcacataa ccaactttta atccagattt ggcagcaacg
     2041 gaattcggtt ccactttagt tactacaaca cctttatcgc ttttcacatt caagcgcttt
     2101 gctgtaggac tatctattgc ttctacagtg attcctgtag caagggttcc tttggttgta
     2161 tctgctgtag cgatagtatc ttcaggatat gcttccagga ttactttcac ggtcatttcc
     2221 tttttattgc gcaagatttt taatgctact tcctgtcctg ctttggcagt agcgatcgct
     2281 atacggaatt ttggaacgct ggtaattttc tgaccgttta tttccagaat aatatcctca
     2341 acctggagac ctgctttttc ggcaggtgaa tctttttcta ctttggcaac aagaactcct
     2401 gcaacttctt tcaaaccgta ggattccata atatcctggg ttatttcctg agccagaata
     2461 ccaatataag cccgggttac ttttccactg gctacaaggt cttctaccac ccgtttggca
     2521 agattgatag gtatagcaaa accgattcct acattgcctc cgttggtgct tgccaaagca
     2581 gcattgatac ctataacttc tcctttgata tttaacaaag gaccgccact attgccactg
     2641 ttgatagcag catcggtttg aataaaatcc tgatagatag gagcattatc acccaaatta
     2701 agattggctc tgctggttgc agaaatcact cccaaagtta ctgttctatc cagtccttcg
     2761 ctgaaaggat taccaatagc aatagcccat tcgccaattt gcagcttgtc cgaatcacct
     2821 aaaggcgcaa cagttacttt ttcaccttct ttaactacaa ttttaataac tgcaacatcg
     2881 gtattggggt ctaaaccaac tactgttgca gtatagacaa ctttatccgc catcgtaact
     2941 gtaattgtac cttccctgcc cttttctact acatggttat tggttaaaat aaatgcttca
     3001 cgggtattag ggttatattc ataaataaaa ccgcttccta aagaaacaat aggacgcgtc
     3061 tgttcctgag gaaaattgaa aaattgacgg aaccagggat cgttaaaaaa gggattaata
     3121 taattctgaa ccgtcacttt tgcttctacc ctaatctgaa ctaccgcatc gcgaacattt
     3181 ttaactactt ctactacagg attgggggca ttagggtcaa tcatattatc ttttgctatg
     3241 gcacaactca ttaagaataa tgccactatc accaggacta tttgtttcca cattttcatc
     3301 attaaatctc cttcttctga aattagattt acttttattt tatacttgcg aaagacaaaa
     3361 aacctgcggg aaaacccgta attaataaaa ttctaaaaac aatttatcct gattagtgtt
     3421 tacagtcaag tggaaaatga agttctttct gaaacaatat tcaataggat tctgcatcaa
     3481 gagcgaaatg acagacgcta acatcatagg caagaatgct atctttgtca ttccggtgca
     3541 agtaggaatc cagtataaaa caaaaaaggg cggaattaac ttccgccctt gctatataat
     3601 tattagtgtc taacccttca actctgc
//
LOCUS       DAOPFL010000128         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_35238_length_3624_cov_102.446904, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000128 DAOPFL010000000
VERSION     DAOPFL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35238_length_3624_cov_102.446904"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLG69_06990"
     CDS             complement(<1..854)
                     /locus_tag="PLG69_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HPI43288.1"
                     /translation="MKQMQILWVDDEIDLLKPFILFLEERKYNVETCNNGTDAIEKVV
                     ENKYDLVILDEMMPGLDGLATLQEIKRINPALPIVMVTKSEAEGLMDKAIASQISDYL
                     IKPINPNQIIMAIKKIFQADEIRANQIGQQYTRYIAELNQKLFANPDWNEWSQIYREL
                     SYWELQIDQVNDEGLRQTHFLEKRNCNTEFTNYVERNYGNWLKSDERPNLSFDLISQY
                     IAPHFEENLPIYFIIIDCMRLDQYLAIQPYIKELFEEELDLYYSILPTATPYSRNSIF
                     SGLMPIDIA"
     gene            complement(861..1481)
                     /locus_tag="PLG69_06995"
     CDS             complement(861..1481)
                     /locus_tag="PLG69_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43289.1"
                     /translation="MPPKTVPPEAMSAEEKFNALANLKDKLEDNFISLGQLLSEIKRS
                     KLYLYKGYENFKDFVEMEYQLSGTLAGKLMSTFDLFIEEMDIDETEVKEIGFDRLQMI
                     KPFMKNADWNVRDEWIHKAEEMPFQELREHIKELKQKEKESNIDLKEVYIEQFWEKMT
                     GWFNCSRKELNFKLALYFQDADLDNIKKIVKERQHLFELETQNKKE"
     gene            complement(1497..2327)
                     /gene="panB"
                     /locus_tag="PLG69_07000"
     CDS             complement(1497..2327)
                     /gene="panB"
                     /locus_tag="PLG69_07000"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPI43290.1"
                     /translation="MTKVKNNKTDIAVFKEMKQHQEKICMLTAYDYAMAKCVSATSVD
                     IILVGDSLGMVVLGYEDTLQVTLENMIYHCSAVRRGAPDSFIIADMPYLSFHLSEKQT
                     RQNAGKLIVQGKANAVKLEGGSKSRIDAIKAIVDMEIPVCAHLGLTPQSIRKFGGYKV
                     QGKKPVEQEELLKQAQEVTKAGAFMLVLEGIPELLGKEITETVSIPTIGIGAGRFTDG
                     QVLVYHDLLGYSDLQPKYVKQYANLNNDIREAVELFSQEVKNGVFPAPENIYYPLIQQ
                     "
     gene            complement(2308..2787)
                     /locus_tag="PLG69_07005"
     CDS             complement(2308..2787)
                     /locus_tag="PLG69_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43291.1"
                     /translation="MLFIFIFSLAFGTAFAQNKSEEYLEVKTWKEPKDAVPFKELVLQ
                     DSIYFYKDKPFTGVAIERYPNGFLQRAATFVKGKQNGPMLMWYPDGSPQMSANYVNGY
                     LQGRFLGWYNNGSVIYDLMINRGTYAGDNLFDEDASRASSEIEDREGESPDNDQSQE"
     gene            3066..>3624
                     /locus_tag="PLG69_07010"
     CDS             3066..>3624
                     /locus_tag="PLG69_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HPI43292.1"
                     /translation="MVKKLKILGIEYALWLYIVACICWFLFRVFSISASSYIIFISPF
                     IIAVLLSQCKNNIGAKIIISEETGSLWKIELAIVILITLIMAWVIVDVKPIAFFTQAA
                     NTKSIISGIFNPNLRQLVPMLSALVETIYLALLATVFAIPFAFVLSFFAAKNLMFSSV
                     IGRIVYIIIRTISTVFRSIEAIVWAI"
BASE COUNT         1026 a          706 c          655 g         1237 t
ORIGIN      
        1 gcaatatcaa taggcattaa accgctgaaa atggaatttc tgctataggg agtagcagtt
       61 ggcaaaatgg aatagtagag gtcaagttct tcctcaaaaa gttccttgat gtagggttga
      121 atagccagat actgatccag acgcatacaa tcaattataa taaaatagat aggcaggttt
      181 tcttcaaagt gcggagctat gtattgggaa ataaggtcaa aacttaaatt ggggcgttca
      241 tcacttttca gccagttacc ataattgcgt tctacatagt tggtaaattc agtattacaa
      301 ttgcgttttt ccagaaaatg ggtttggcgt aaaccttcat cgttaacctg atctatttgt
      361 aattcccaat agctcagttc tcggtaaatt tggctccatt cattccaatc gggattggca
      421 aaaagttttt gattcagttc tgcaatatag cgggtatatt gttggcctat ttgattggca
      481 cgaatttcat ctgcttggaa aatctttttg atagccataa taatctgatt gggattgatg
      541 ggtttaatca aataatccga aatttgagaa gcaattgcct tatccatcaa gccctctgct
      601 tcacttttag taaccattac aataggcaag gcaggattga tacgcttaat ttcctgtaaa
      661 gttgccaagc catccaagcc gggcatcatt tcgtctaaaa taacgaggtc atatttattc
      721 tctactacct tttcaatagc gtcggttcca ttattacagg tttcaacatt atattttctc
      781 tcttccagga agagaataaa gggtttgaga aggtctattt catcgtcaac ccagagtatt
      841 tgcatttgtt tcattggatt ttactccttt ttgttttgtg tttcaagttc aaagaggtgt
      901 tggcgctctt taacgatctt tttaatgttg tccagatcgg catcttgaaa ataaagggcg
      961 agcttgaaat taagctcttt gcgcgaacaa ttaaaccatc ctgtcatctt ctcccaaaat
     1021 tgttcaatat agacctcttt caggtctatg tttgattctt tttctttttg tttcagttct
     1081 tttatatgct cgcggagttc ctgaaatggc atttcctccg ctttatgaat ccattcatca
     1141 cgcacattcc agtccgcatt tttcataaag ggtttaatca tctgcagacg atcaaaaccg
     1201 atttctttaa cctcggtttc gtctatatcc atttcttcaa taaacagatc aaaggtagac
     1261 ataagttttc ctgccagggt tccggaaagt tgatattcca tttccacaaa gtccttaaaa
     1321 ttctcatagc ccttataaag gtataatttt gaacgcttga tttcggaaag caactgaccg
     1381 agagagataa agttatcttc cagtttatct ttcagattgg caagtgcatt aaatttttct
     1441 tcggcactca ttgcctcagg tggcactgtt tttgggggca tcttttttat ccttctttat
     1501 tgttgaatga gggggtaata aatattttca ggagcaggga aaactccgtt tttaacttcc
     1561 tgggaaaaga gttctaccgc ttccctgatg tcattattta aattcgcata ttgttttaca
     1621 tatttgggct ggagatctga ataaccaagc aggtcgtgat aaactaaaac ctgtccatcg
     1681 gtaaatcttc cggctccgat tccgattgtg ggaatgctta ccgtttcagt tatttctttg
     1741 cctaaaagtt caggaatgcc ttccagaaca agcataaagg cacctgcttt ggtaacttcc
     1801 tgtgcttgct ttaatagttc ttcctgttca accggtttct ttccctgaac tttatatccg
     1861 ccaaatttac gaatggactg tggtgttaaa cctaaatggg cacaaacagg tatttccata
     1921 tccacaatgg cttttatggc atcaatacga gattttgagc caccttccag tttaacggca
     1981 ttagctttac cttgcacaat aagctttcct gcattttgcc tggtttgctt ttcggaaaga
     2041 tgaaagctaa gatagggcat atcggctata ataaaagaat ccggtgcacc cctacgaact
     2101 gccgaacaat gataaatcat attttccaat gttacctgca gggtatcttc atagcccaaa
     2161 actaccatcc ccaaagaatc acctaccaga atgatatcta cggaagtggc agaaacacat
     2221 tttgccatcg cataatcgta ggcagtaagc atacagattt tttcttggtg ctgtttcatt
     2281 tccttaaaaa cagcaatatc tgttttatta ttcttgactt tggtcattat cgggactttc
     2341 tccttcccta tcttctattt cagaacttgc gcggcttgca tcttcatcaa aaagattatc
     2401 accggcataa gttcccctgt taatcattaa atcataaata acactgccat tattatacca
     2461 gcccagaaaa cgcccctgta aataaccatt tacataatta gcactcattt ggggactgcc
     2521 atcaggatac cacatcagca taggaccgtt ctgtttacct tttacaaaag ttgctgcccg
     2581 ttgtaaaaaa ccattgggat agcgttctat ggcaactcct gtgaagggtt tatctttata
     2641 aaaataaata ctgtcttgca aaacaagttc cttaaaagga accgcatctt tcggttcctt
     2701 ccaagtttta acctctaaat attcttcact tttattctgt gcaaaggcag tgccaaaagc
     2761 aagggaaaat ataaaaatga atagcattaa aggcattacc gggaagttta ggaagccggt
     2821 tttttttcgg acacaggata aggaacgcat attatttatc acttgaagac acatttttat
     2881 ctccgatagg ttagtcaatc tttttcttct tttttttaac tgaagctttt tattatataa
     2941 gataaaccaa attatcttaa agtcaaccga aaggttgatg tcctcatcaa gcacaaaacg
     3001 acaggaatgt cgttcttccg gagttttttc cttgacctaa taaatcactg tgtaaaaata
     3061 gttttatggt gaagaaacta aaaatattgg gtattgaata tgccctctgg ttatacatag
     3121 ttgcctgtat ctgctggttt ttatttcggg ttttcagtat ttccgccagt agctatatta
     3181 tttttatcag tccgtttatc atagcagttc ttttgtctca atgcaaaaat aatataggag
     3241 caaaaataat catctcggaa gagacaggtt cactgtggaa gattgagctg gcaattgtaa
     3301 tccttattac tttaattatg gcttgggtaa ttgtagatgt aaagccaatc gcatttttta
     3361 ctcaggcagc caatacaaag tccattatca gtggaatatt taacccgaat ttgagacagc
     3421 ttgttccgat gcttagtgct ttagtagaaa ctatttatct ggctttattg gcaactgttt
     3481 ttgctattcc ttttgccttt gtgctttcct tttttgctgc caagaatctg atgttcagtt
     3541 ctgttatcgg caggatagtg tatattatta tcagaacgat ttctactgtc ttcagaagta
     3601 ttgaagcaat tgtgtgggca atta
//
LOCUS       DAOPFL010000129         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_35799_length_3583_cov_106.845805, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000129 DAOPFL010000000
VERSION     DAOPFL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35799_length_3583_cov_106.845805"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLG69_07015"
     CDS             complement(<1..650)
                     /locus_tag="PLG69_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43293.1"
                     /translation="MKFRKLETLPLALILIFLCVSISPCLAQFAGGTGSEAAPYLVAT
                     AQHLNNVRNYPGAFFLQTDDIYLNVEPYNSGFGWLPIGTQSAPFSGSYDGNNFHIYYL
                     YLNQSEGNNLGLFGYAHSAVLKNITLQDVYINGKDYVGSLIGSATECFLESCSSEGTI
                     TGASVTGGLVGSLELSCHLEKCFANCVVSGQFATGGLCGYSKGASEIYSSYSKGTVT"
     gene            complement(793..2127)
                     /locus_tag="PLG69_07020"
     CDS             complement(793..2127)
                     /locus_tag="PLG69_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43294.1"
                     /translation="MGQYLARCKFSFLFILTFCAGLTLNADWRMDFCLANLGSDGSLI
                     SATVYLGESAGAADSFDAFDFPLGPPPSNNPYVRAYFPHSDWGTTYNGNYMKDIRSIN
                     PAAKTWNLTFSKNGTPLSNNYRLSWEITGLFPACYQPRLIFNSQNINMLTQSSYSYTG
                     WINSGSIQMQYNNSVPYQLEPLPELLFSNNLTQELNLNRYFKVGSGTLSFSFLPNNNL
                     VQNMVTNQDSVWLNIYPVAGFTGTTVAEFSVIGTGGTLNAQISITRDATNSPPVFRDT
                     IQPLEIIQNQQGIFSWNGMVFDPDLDTVTINFSGGENISIEPNETLQQAIVIPALGFK
                     GETNFQFSLADNLHSPVIYAIPVTVLPAVPTSPQNLVLQLLPENSLLLTWQSVHTDTF
                     GLPISGLKYKVMLYADSAGTQLIHQYTNLTSSDILIPVESERMFIKVIAVNE"
     gene            2697..>3583
                     /locus_tag="PLG69_07025"
     CDS             2697..>3583
                     /locus_tag="PLG69_07025"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPI43295.1"
                     /translation="MERYSKLLPGMVEWTLQKRAEDNVSESFGCGYLFVDVCDFTKLT
                     ESASVKGHYGVEIITDILNQYFDLLNEKIMQYNGQIIKFEGDAVLAAFPGKEEICLCQ
                     MQECLKEFNRELQELNKRLKNKYGSDLSYHSSMGYGQSNVIILGKPNIHYDYFVYSPV
                     MPSLYNLFAQAGKNECLQVKSGIKTKKKAEIYGAELPLRAEKSYDQSFFPPEILQRIS
                     SQTFTGELRNAAILFIGIAAEKYIQKGDYKTINNYYCKVQEIVYRLEGMINKIDYTDK
                     GLILLISFGILQTHIDDIER"
BASE COUNT         1190 a          648 c          715 g         1030 t
ORIGIN      
        1 gtaacagttc ctttgctgta agaagaataa atttcactgg cacctttact atagccgcaa
       61 agtccaccgg tagcaaattg accgctgacc acacaattag caaagcattt ttccagatgg
      121 caggatagtt ccaaagaacc aactaaacca cccgttacag aagcacctgt aattgtgcct
      181 tcgctgctgc aactttcaag aaaacactct gttgctgagc caatcaggga gccaacatag
      241 tccttaccgt tgatgtatac atcctgtagg gtaatatttt tgaggacagc agaatgggca
      301 taaccaaaca aacccaaatt atttccctcc gattgattaa ggtataggta gtaaatatga
      361 aaattattgc catcgtaaga accgctaaaa ggagcggact gagtgcctat aggtaaccag
      421 ccaaaccctg aattataagg ttcaacattt aaataaatat cgtcggtttg taaaaagaac
      481 gcgccaggat aattccgcac attgtttaaa tgttgtgcag ttgcaaccaa atagggagct
      541 gcctcagaac ctgttcctcc ggcaaattga gccaaacaag ggctgataga tacacagagg
      601 aaaattaaaa taagtgctaa aggcaaggtt tcaagttttc ggaatttcat cgtatcctcc
      661 agaaattata ttagtttttg gtttatagtt aaaattcatc gtttttttgc cggttataat
      721 ctcgcagatt aggttttttc atattcatag aataacttaa gtttatctgt ctgcttttca
      781 tagtgttcac gcttattcgt taactgcaat aactttaata aacattctct cactctctac
      841 aggaataaga atatcagaag aggtaagatt agtatattga tggattagtt gagttccggc
      901 gctatcagca tagagcataa ctttgtattt aagcccgcta ataggcaaac cgaaagtatc
      961 agtatgaact gattgccagg taagcagaag agagttttcc ggtaaaagtt gcagaaccaa
     1021 attttgagga ctggtaggaa cagcaggcaa aacggttact ggaattgcat aaataaccgg
     1081 ggaatgcaaa ttatccgcaa gagaaaattg gaagttcgtt tcacccttaa aaccgagagc
     1141 cggaataaca attgcctgtt gcaatgtttc attgggttca atgctgatat tttctccccc
     1201 ggaaaaattg attgtaaccg tatccaaatc gggatcaaaa accattccgt tccaggaaaa
     1261 aataccctgt tgattttgga taatttccaa gggttgaata gtatccctaa aaacgggcgg
     1321 tgaattagta gcatcacgag taatagaaat ttgggcgtta agggttccac cggttccaat
     1381 aacactaaat tctgcaacgg ttgttcctgt aaaaccagct acaggataaa tatttagcca
     1441 cacagaatcc tgattagtaa ccatattctg cactaaattg ttattgggaa gaaaggaaaa
     1501 agagagagtg ccactgccta ctttaaaata tctgttcaga tttaactctt gggtaaggtt
     1561 attggaaaaa agcaattccg gtaagggttc caattgatag ggcactgaat tattatattg
     1621 catttggatg gaaccggagt taatccaacc tgtgtaagaa tagctgctct gcgtaagcat
     1681 attaatattt tggctgttaa aaattaaccg gggctgataa caagcgggaa aaagtcctgt
     1741 tatctcccag gagagacgat agttattact taagggagtg ccgtttttag agaaagtaag
     1801 attccaggtc ttggcagcag gattgataga cctgatgtct ttcatataat ttccgttgta
     1861 agttgtgccc caatcgctgt gaggaaaata ggcacgcaca taaggattat tactgggagg
     1921 aggaccaaga ggaaaatcaa aagcatcaaa actatccgca gcacctgcgc tttctcctaa
     1981 ataaacagta gcacttatta gggagccatc agaacccaaa ttggcaagac aaaaatccat
     2041 tcgccaatcg gcatttaaag ttaaccctgc gcaaaatgta agaataaaaa gaaaagaaaa
     2101 cttacaccgc gctaaatact gtcccatata atcaccttcg tcagcattta tctataattt
     2161 actttactaa tccttcagct ttttctgtca agaaatttct gaaaaaggaa aaagatttat
     2221 attaaatttg tcttagtcaa ccccgatatg attcccttat gattcccata tcgttcccat
     2281 atcgcgatat gggaagcgta cgggaaagtt ttggtcaata taacagctaa ctgcaacggc
     2341 tacaaaaaga taaagaaaca caggaaaagg taaattgaga gggcaagata gcgaaatagg
     2401 gaaggagaaa aaggtaactt ataaaaggat ttcataaaca ggtaaagcag taagtatcct
     2461 gctgtcaggt tgatttatat ttctgctttg cctccttgca aaaaaaattt cttttctctt
     2521 ttccttttta tttacaagct atcatttttg gtgtattcgg tattcccggt tttgtttaca
     2581 agtaatcatc ttcggttttt tcggtattta gggtagctaa accaaaccct aatctttttc
     2641 cttgacctgt aaagtggatt ttataaaaga taaaaaggtc ttaaaaggga gacaatatgg
     2701 aacgctacag taaattatta cccggaatgg tggaatggac actgcaaaaa agagcagaag
     2761 ataatgtatc ggaaagtttt ggttgcggtt atctttttgt agatgtttgc gattttacaa
     2821 aattgacaga aagcgcttcc gtgaaaggac attacggagt agaaattatt accgatatct
     2881 taaaccagta ttttgatctc ttgaacgaaa aaataatgca atataatgga cagattatca
     2941 aatttgaagg tgatgctgtt ttggctgctt ttcccggtaa agaagagatt tgcctatgcc
     3001 agatgcagga atgcttaaag gaatttaacc gggaactgca agaactgaat aaacgcctga
     3061 aaaataaata tggcagtgat ctttcctatc atagcagtat gggttacggt caatccaatg
     3121 tgattattct gggcaaaccg aatatccatt atgattattt tgtgtatagt ccggtaatgc
     3181 cttcccttta taacctcttt gcccaagcgg gaaaaaacga atgtttgcag gtaaaaagtg
     3241 gcataaaaac gaaaaagaaa gccgaaattt atggggctga acttcccctg cgagccgaaa
     3301 aaagctacga tcaaagtttc tttcctccgg aaattttaca gcgcatttct tcccaaacat
     3361 ttaccggtga actgcgaaat gccgctattc tctttattgg tattgctgcc gaaaaatata
     3421 ttcaaaaggg tgactacaaa accattaaca actactattg taaggttcag gaaattgttt
     3481 accgtctgga aggaatgatt aacaaaattg actataccga taagggtttg attttactta
     3541 tcagtttcgg cattttacaa acccatatag atgatattga gcg
//
LOCUS       DAOPFL010000130         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_35800_length_3583_cov_76.149660, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000130 DAOPFL010000000
VERSION     DAOPFL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35800_length_3583_cov_76.149660"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1224
                     /locus_tag="PLG69_07030"
     CDS             109..1224
                     /locus_tag="PLG69_07030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI43296.1"
                     /translation="MKKVLHIQLLPILSGVQRFSLHLLDGLDRNEFEIWIACKPGGEF
                     VDEIKVRGYRYLPLPTFRHQICVWDIFTFFHLLYLIKKHHFDIVHTNASKPGLLGRLA
                     ARLGKVPLIIHTTHTFPFLEHQKLVTYKFFTTLEKIGNRLGDYNVFVNNSDRLKSLRM
                     GLIKPEKAITIYNALPLGLTSTLQEIAQNRKKPEKEIIIGSTLRFCLQKNVINLITAS
                     CNACLQEPKLKFIYLGEGEYFELCKAIVHSYGLEERIILPGWDKDVLPWLKVFNVFIL
                     YSRWEAMPFSIIEAMHSGLAVIVSDLPSLSELVDSETGYIVPLDDSANLIQTFREIAS
                     DFEMPYQKGKKAAKKIAKICSYEKMVNAYRELYLSAK"
     gene            1237..2283
                     /locus_tag="PLG69_07035"
     CDS             1237..2283
                     /locus_tag="PLG69_07035"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPI43297.1"
                     /translation="MINIYLLIIIQAVLIHLCTHLLFPLNLKISKKLKLVAYPGERKI
                     HKIPIPEAGGLCFALPIILAQATFGLFSGNAEMGKMLLELSGVGFLALCFGVWDDRFE
                     SPPPYKLVWQICLGVIMYLFGYRVLYLTNPFGEHFILGWLSFPVTVLWYLIVINAINF
                     IDGLDGLACGITIIVSAVLIVIGIKEQNELVIAISSFLLAGNLAFLRYNFYPAKIFMG
                     ETGALFIGLNIAAISTAGTSQYKGITSITLMVPLAVMAVPLIDFVLAVFRRLHYRDMF
                     HSDKEHIHHTMLDIGFSQRAIAIIIYLVTLLFGLIAIGFSFSSKKIVFSLLLGLFALM
                     VVIAYLIMRQERKK"
     gene            2280..>3583
                     /locus_tag="PLG69_07040"
     CDS             2280..>3583
                     /locus_tag="PLG69_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43298.1"
                     /translation="MKRFFLLVTLLISVFLTAQNWKTFTNTNHIYDLCMKDNNLYVAT
                     WGGVLKISPIDNSNNLSAYTEKEIYHKGNGLVSNDIRTLSYIDFSESLWMGSSDNGIS
                     ISSQLGFQNLGSALGLPSLKVTRIIEHESTILVATSSGLAVFYYLPGVAFPLMLHQYT
                     VANTSGGLAGDNIVDMLLTETGTLFVATTNGVSYIPLEQLDNDSAWKTISGPGSPLLS
                     GGNPKLSANSENIAIGIYKEVYVHSLNMDSGSWTTYNVSNMLTGKNVSSVLLDAQSRL
                     WISYADWNEAILSYTNTSDSLLTLIENNTYTHILKETNGLGYSPISRIVELNGEIYLC
                     SWGQGIYHQEENYWSNYDPTGIGFPRIAQVVTDNDYNLWFASGYISNLPVRKGSMGVS
                     KLVNGNWSTLNIHNSPIHTDNILGVAVDPLNRKWFATWDNTN"
BASE COUNT         1046 a          578 c          727 g         1232 t
ORIGIN      
        1 ataggtaggg gatgaacagg atgaaagaga tttacaggat ttagaatata agttccggag
       61 gaacgacaga taataacgac ggggaaattg ttttccaggg attaaaagat gaaaaaggtg
      121 ttacatatac agttactacc aatactatcc ggggtgcaga gattttcttt gcatcttttg
      181 gatggcttgg atagaaatga atttgaaatc tggattgcct gcaaaccagg tggagaattt
      241 gtggacgaaa taaaagttag gggttatcgt tatttaccct tacccacttt tcgtcatcag
      301 atatgtgttt gggatatttt tacctttttt cacttgcttt acttaattaa aaagcaccat
      361 tttgatattg tgcatactaa tgcttccaaa cctggattat taggtcgttt agctgctcgt
      421 ttaggtaaag ttcctttaat tattcatacc acacatactt ttccttttct ggaacatcaa
      481 aagctggtta cctacaagtt ttttaccact ttggaaaaga taggcaaccg attgggtgat
      541 tacaatgttt ttgtgaataa ttccgatcgg ctaaaatcct taaggatggg tttaatcaag
      601 ccggaaaaag caatcactat ttacaatgca cttccgttag gattaacttc tactttacaa
      661 gaaattgccc aaaaccgcaa aaaaccggag aaagaaatta ttattggttc tactttgcgt
      721 ttttgtctcc aaaaaaatgt aatcaattta ataactgctt catgtaatgc ctgtttgcag
      781 gagccaaaat taaaattcat ctatctgggt gagggtgaat attttgagct ttgtaaagca
      841 atcgttcaca gttacggttt ggaagaacgc attatacttc cgggttggga taaagatgtt
      901 ttgccttggc taaaagtttt caatgtcttt attttatatt cccgttggga agcgatgcct
      961 tttagtatca ttgaagcaat gcattcaggg ctggcagtaa ttgtttcaga cctgccctct
     1021 ctttcagaat tggtagatag tgaaacaggt tatattgttc ctttagatga tagtgctaat
     1081 ttgattcaga cctttaggga aatagcaagt gattttgaaa tgccttatca gaaagggaaa
     1141 aaagcggcaa aaaagatagc caaaatttgt tcttacgaga agatggttaa tgcttacaga
     1201 gaactttatc tatcagcaaa ataacagagc agcataatga taaatatata cttactaata
     1261 ataattcaag cggtgttgat tcacctttgc acccatttgc tttttccgct gaatttgaaa
     1321 atttctaaaa aactgaagct cgttgcctat ccaggtgagc gcaaaattca taaaataccg
     1381 attccggaag cagggggctt atgttttgct ttgccaatta ttttagcaca ggcaactttt
     1441 ggtttgttta gtggaaatgc agagatgggt aaaatgcttt tagaactttc cggagtagga
     1501 tttctggcat tatgtttcgg ggtttgggac gatcgttttg aaagtcctcc tccttataag
     1561 ctcgtgtggc agatttgttt aggcgttatt atgtatttgt ttggttaccg ggttttgtat
     1621 ctgaccaatc cattcggaga acattttatt ttgggttggt tatcttttcc ggttactgtt
     1681 ttatggtatt taatagtaat caatgcgatc aattttatag acggtctgga tggtttggcg
     1741 tgtggcataa cgattattgt tagtgccgtt ttgatcgtaa tcggcatcaa ggaacagaat
     1801 gaactggtaa tcgccatttc ttcttttttg ctggcaggaa atttagcttt cctgcgctat
     1861 aatttttatc cagcaaagat atttatgggt gaaaccggtg ctttatttat cgggttgaat
     1921 atagctgcaa tttccactgc tggaacttca caatataagg gaataacttc tatcaccctg
     1981 atggttcctt tagcagttat ggctgtgcct ctaattgatt ttgttttggc tgtttttcgg
     2041 cgtctgcatt atcgggatat gtttcattcc gataaagaac atattcatca tacgatgttg
     2101 gatattggtt tttctcaaag agcaattgcg attataatat atttagtaac cttgcttttt
     2161 ggacttattg ccattggctt ttctttttcc tccaaaaaga ttgttttttc cttactttta
     2221 gggctttttg cattgatggt agtaattgcc tatttaatta tgcgacagga gcgaaaaaaa
     2281 tgaaaaggtt ttttctgctt gtaaccttgc tgataagtgt ttttttaact gcacagaact
     2341 ggaaaacatt taccaataca aatcatattt acgatttatg tatgaaggat aataatcttt
     2401 atgttgctac ctggggtgga gtgctaaaaa ttagtcccat agataattct aataatttaa
     2461 gtgcttatac cgaaaaagaa atttaccata aaggtaacgg attagtttct aacgatatta
     2521 gaaccctttc ctatattgat ttttccgaaa gtttatggat gggctcttcg gataatggaa
     2581 tttcaatttc aagtcaactt ggctttcaga acctgggaag tgctttagga ttgccttcac
     2641 taaaggtaac caggatcatt gagcacgaat caacaattct tgtggcaact tcatctggac
     2701 ttgccgtttt ctattatctt ccaggtgttg cttttccttt gatgcttcat caatatactg
     2761 ttgccaatac ttctggaggt ctggcaggag ataatattgt agatatgtta ttaaccgaga
     2821 caggaactct ttttgtggct acaactaatg gagttagcta tattccactg gagcaattgg
     2881 ataatgattc tgcctggaaa actattagtg gacctggcag tcctcttcta agtggtggaa
     2941 atccaaaact ttctgccaat tctgaaaaca ttgcaattgg catatataaa gaggtctatg
     3001 tgcattcttt gaatatggat agcggttcct ggacaactta taatgtgtct aatatgctaa
     3061 ccggaaaaaa tgtctcttca gttctgttgg atgcacaatc gcgattatgg atttcttatg
     3121 cggattggaa tgaagcaata ttaagttaca caaatacaag cgattcactt ttaactttaa
     3181 tagaaaataa tacctatacc catattttaa aagaaacaaa tggacttggt tatagtccaa
     3241 tcagcagaat agtagaattg aacggtgaaa tttatctttg cagctgggga cagggtatct
     3301 atcatcagga agaaaactat tggagtaatt atgatccaac cggtattgga tttcccagaa
     3361 ttgctcaagt tgttactgat aatgactata acttgtggtt tgccagtggt tatatttcta
     3421 accttccggt gcgtaagggt tcaatgggtg taagtaaatt agtcaatggc aactggtcta
     3481 ctttgaacat tcataacagt cctatccata cggataatat tttaggtgtt gcagttgatc
     3541 ctttgaacag aaaatggttt gccacctggg ataatacaaa cag
//
LOCUS       DAOPFL010000131         3503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_36906_length_3503_cov_92.327436, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000131 DAOPFL010000000
VERSION     DAOPFL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36906_length_3503_cov_92.327436"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..237)
                     /locus_tag="PLG69_07045"
     CDS             complement(43..237)
                     /locus_tag="PLG69_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43299.1"
                     /translation="MKGKLPSGFQTNRIIWAKTNRRYLTVMPTEFLLQYMIKERRNNV
                     NYYFYSLSSNSITEALLTHY"
     gene            454..1251
                     /locus_tag="PLG69_07050"
     CDS             454..1251
                     /locus_tag="PLG69_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43300.1"
                     /translation="MKKTVWLIIAIVILMGLVGCKKKEEKLPSQETASAITTYAPLPS
                     FKDVFRVLDQLQAKDISAAVPADLYKTKQEEVRNAFSLGLLTADATLAAKGHNKKRLS
                     QISSKMMDLTTLLGLEEEVNQMSAELKNLLEKEQWTELETALDNHKKKVEDKLWEMES
                     FDNYTLMLLGGWIEATNRVAWLIKQDYSAEKTKALNQKGTFNSLIGNLKNIKAEHITS
                     QPEFQEVLGLMEQLKAVIDADKEKTYTPEQLDQIISLTNQIKETFKK"
     gene            1443..2543
                     /locus_tag="PLG69_07055"
     CDS             1443..2543
                     /locus_tag="PLG69_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43301.1"
                     /translation="MKKVILISGILFFSTLLFAQSTFFWIEDVPWDDGKALNIKANVP
                     ADTVYLQKALEDGTWETIYTGKGRSLSFTQNELNPDIKYRYRLLVPRPAFNDTIFFMA
                     EAQPKEQWFYLHKLSYLLILLAVSIAIIYYVQNARKGKDYYIRRISGLDCIEDSIGRA
                     TEKGKPILFVPGIMDLDDIQTISSLTILSHLAHKAAEYDTEIIVPCRFSMVLSAAREV
                     VKEAYIKAEKPDAYNQDRIFYLTDDQFGYVAGIDGIMVREQPAANFFLGSFYAESLIL
                     AETGFSTGAIQTAGTAQASQLPFFVVSCDYTLIGEELFAASAYLSRDPQQLGSLKGHD
                     FGKLLVILLIIAGVITEMSGWHWFKAFFEGAQ"
     gene            2540..3238
                     /locus_tag="PLG69_07060"
     CDS             2540..3238
                     /locus_tag="PLG69_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43302.1"
                     /translation="MKYKIPLVFAFVCGILVVISEFIPHRPFNMLITTLQDWFMIISG
                     FAILLGQISLFQANIKKIKEKNKDWPFYLAGLISFTVMLIFGLLWGTQEYRGIFGQGE
                     KIQATVGMKPFDYLFANAFMPLSSTMFSLLAFFIASAAYRAFIIRSFESNLLMITAVI
                     VMLGRTSFGGMLTSWLPEGLKFLHLPNLSDFIMEFPNSAAQRAILISAALGIIGSSLR
                     IILGIERSYLGGEK"
     gene            3235..>3503
                     /locus_tag="PLG69_07065"
     CDS             3235..>3503
                     /locus_tag="PLG69_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43303.1"
                     /translation="MSANKNTQTERRIIYLVLLVAVALPLIFPIGWKTEVSSYTKMAW
                     ELIENTPDSSVVLFSFDYDPSTITEIQPMAEAMLEHSFSRNQKIIA"
BASE COUNT         1060 a          623 c          681 g         1139 t
ORIGIN      
        1 taatagcatt gcttacccat tgcttaccca ttacggatgg cattagtaat gggtaagcaa
       61 tgcttcagta atgctattgg atgaaagaga atagaaataa tagttaacat tatttctcct
      121 ctctttaatc atatattgta gtaaaaattc tgtaggcatt acagttagat aacgacggtt
      181 tgttttagcc caaataatgc ggtttgtttg aaagcccgaa ggtaatttcc ctttcatttc
      241 ttttcaatac attattggag tttaacatta caattttggg ctttgataaa tatgctttat
      301 cttgattgat gaggacttca accttccgag tagttttcct tttttccttg acaattcttt
      361 atccttggta aacaaagaaa aaaagttaat aagacccttt tttatcagac ataagctttt
      421 gctaaatgat gaattagata aaaggagtaa acaatgaaaa aaacggtttg gttaattatt
      481 gccatcgtaa ttttgatggg tctggttggc tgtaaaaaaa aggaagaaaa attacccagc
      541 caggaaacag catccgctat cacaacttat gcccctctgc cttcatttaa agatgttttt
      601 agagttctgg atcaacttca ggcaaaagac atttcagctg ccgttcctgc tgatctttat
      661 aaaaccaagc aagaagaggt tcgtaacgct ttttctttag gtttactaac ggcagatgct
      721 actttagctg ctaaaggtca taataaaaag cgccttagtc agatttcttc caagatgatg
      781 gatttaacca ctcttctggg tttggaggaa gaagtaaatc aaatgagtgc cgaactgaaa
      841 aatttgcttg aaaaagaaca atggactgaa ctggaaaccg ctttggataa tcacaagaaa
      901 aaagtggaag ataagctctg ggaaatggaa agttttgata actacacttt gatgcttctc
      961 ggcggttgga ttgaagctac caatcgcgtt gcctggctta taaaacagga ttactctgca
     1021 gaaaaaacca aggcattaaa tcaaaaaggc acattcaata gcttaatcgg caatttgaaa
     1081 aatatcaaag cagaacatat taccagtcaa cctgaatttc aagaggtttt aggtttgatg
     1141 gaacagctta aagcggttat tgatgccgat aaagaaaaga catatactcc ggaacagctg
     1201 gatcaaataa tttcgctgac taatcagatt aaagagacct ttaaaaagta atttctgcta
     1261 ttattatgtt acaatcaggt tgggaaaaag caagatttaa ggattgctga tattactttc
     1321 ccaaccttat tatctatggc tgttctattg aattctgatg gaacagatac agaataatgg
     1381 atttttttga actttgtggc aaattgttgt ttgtcatcct gcaaaaaact aaaaggaaga
     1441 taatgaaaaa agtaatacta atttcgggta ttctattctt ttccaccctg ttatttgccc
     1501 aatccacctt tttttggatt gaagatgttc cctgggatga tggaaaggcg ctaaacatta
     1561 aagcaaatgt tcctgccgat actgtctatc tgcaaaaagc tttggaagat ggaacctggg
     1621 aaacaattta cactggtaaa ggaaggtctc tttcctttac gcaaaatgaa cttaatcctg
     1681 atattaaata cagataccgt ttgctggttc cgcgtcctgc atttaatgat accatatttt
     1741 ttatggcaga agctcaaccc aaggaacaat ggttctatct gcacaagctt tcctatcttt
     1801 taattctgct tgctgtttcc attgccatta tctattatgt tcaaaatgct cgcaaaggaa
     1861 aggactatta tattcgcagg attagcggtc tggattgtat tgaggattcc attggcaggg
     1921 ctacggaaaa aggaaaaccc attctttttg ttcccggaat tatggatttg gatgatattc
     1981 aaaccatttc cagtttaacc atcttaagcc atttggctca taaagcggcg gaatatgata
     2041 cggaaataat tgttccctgc aggttttcta tggttttatc cgctgccaga gaagtagtaa
     2101 aagaagcata tatcaaagcc gaaaagcccg atgcttacaa tcaggacagg attttttact
     2161 taaccgatga tcaatttggt tatgtagcag gaattgatgg aattatggta cgcgaacagc
     2221 cagcagccaa ttttttcctc ggaagttttt atgccgaatc cttaatttta gccgaaaccg
     2281 gttttagtac aggcgcaatt caaacagctg gaaccgctca ggcaagtcaa ttaccctttt
     2341 ttgtggtttc ttgtgattac actttgattg gagaagaact ttttgccgct tccgcttatc
     2401 tttcgcgtga tccgcaacag ctgggttctt taaagggtca tgatttcggt aaacttttag
     2461 taattctatt aattatagct ggagtaataa cagaaatgtc tggctggcat tggtttaaag
     2521 cattttttga gggggcacaa tgaagtataa aatccctctt gtctttgcct ttgtatgtgg
     2581 aattctggta gttatttccg aatttattcc ccaccgtcct ttcaatatgc tgattaccac
     2641 tttgcaggat tggtttatga ttatttccgg ttttgccatt cttttagggc aaatcagttt
     2701 gtttcaggca aacattaaaa aaatcaaaga aaaaaacaag gactggccct tttacctcgc
     2761 gggtctgatt agttttactg taatgttaat ttttggcttg ctgtggggaa ctcaggaata
     2821 tagaggaatt tttggtcaag gagaaaaaat ccaggcaacg gtaggaatga aaccctttga
     2881 ttatctgttt gccaatgctt ttatgccact atcgtcaaca atgttttccc tgctggcatt
     2941 ttttattgct tcagctgctt atcgtgcttt tattattcgt agttttgaat ccaatctatt
     3001 aatgattacg gctgtaatag taatgttggg aagaaccagt tttggtggaa tgcttacttc
     3061 ctggcttcca gaagggctta aatttttaca tcttcccaat ttgagcgatt ttattatgga
     3121 atttcccaat tctgcagctc aaagggcaat tctgattagc gctgctttag gaataatagg
     3181 tagcagttta cggattattc ttggtattga aagatcctat ctgggaggcg aaaaatgagc
     3241 gctaataaaa atacccaaac cgaacgcaga ataatctatt tagtcctttt agttgcagtt
     3301 gctctgcccc ttatttttcc tatcggctgg aaaacagagg tctccagcta cacaaaaatg
     3361 gcttgggaat tgatagaaaa tactcccgat tcgtctgttg tcctcttttc ctttgattac
     3421 gatccttcta cgataacgga aattcaacca atggcagaag caatgctgga acattctttt
     3481 tcccgcaatc aaaaaattat tgc
//
LOCUS       DAOPFL010000132         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_37090_length_3490_cov_57.806987, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000132 DAOPFL010000000
VERSION     DAOPFL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37090_length_3490_cov_57.806987"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLG69_07070"
     CDS             <1..752
                     /locus_tag="PLG69_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43304.1"
                     /translation="LQMETNRDFSAAKESYIFLFESYRSFADTKGMLDCLSGLARIEL
                     ENENEKAYLQYKNQTAEIIQNIAPELSYYLLLLELYNYQLENNYSAISQMAINKLDYP
                     DEVNLKLAIDKLQADSYLAIAEEKQVEELINLSKKIEKQQKKTKYNNPELLSSAWYAL
                     SYYYFRTADYKTANFYAHKAQDWNYRFGNFPGMAHCLWLKAQISAAENNIPAAKSYWK
                     MAEGIFSSLQDNVSLEELNHTRQKMLKEQNK"
     gene            834..2111
                     /locus_tag="PLG69_07075"
     CDS             834..2111
                     /locus_tag="PLG69_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPI43305.1"
                     /translation="MKLLKSVSLPTSSTALKTVNVLFADKIIKISTEEIEVEEDVQII
                     NAKGLLMLPGGIDPHTHILTQNADSATDLAKITKIALEGGWTTLSELSYHTQSPVFNN
                     SALKKKIALVNANSYTSLALWGHIDINDYPYHSESAQEIWNKGVVGIALAVPSPNPAI
                     ADITFPEIMDLFFDIYESDTSFAFQGYDYESYREFSPLAQLEAIKKILRRMQENPIHI
                     PRISSYPTIEFINSISKRSDISYATCIIDLMALFNPEVFSTPFLCDYADFADLLFDLL
                     RSNKIYLLSNCVAPPVKNPNYCELFQGCNPELMNYSYLWVLSELWKKRKVPLATCLKM
                     TSENAAKRLGIYPQKGSLDPGSDADFVLYDPESTTLIKGNKNKDIELSGSFKAVYLKG
                     NQVAGKNFETLRQGSFLPRSTNPKRRHNNSSWI"
     gene            2943..>3490
                     /locus_tag="PLG69_07080"
     CDS             2943..>3490
                     /locus_tag="PLG69_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43306.1"
                     /translation="MKKGLFILLALIVASSVFAFTKGTINPGGTVSFTSNKPNSDEKA
                     ITMFSITPQIGYFVMDNMSADVLINYTYEGQSESDYNDEYSLSNLGLGIGGRYFYPLE
                     PGEIYGGLDFLYNSFSWESGPNDGGRNAMYLGLKGGYLFPVVENVYVDFGLKYQMGLG
                     DYGGDDFEDVPDEYKENEESTFG"
BASE COUNT         1125 a          620 c          611 g         1134 t
ORIGIN      
        1 ctttgcaaat ggaaaccaac agggattttt ctgctgctaa agaaagctat attttcttat
       61 ttgaaagcta tcgttctttt gctgatacta aaggtatgct tgactgctta agcggtttgg
      121 caagaataga attggaaaat gagaatgaaa aagcttattt gcaatacaaa aatcaaacag
      181 cagaaataat tcaaaatatt gctcctgaac tttcttacta tcttctgctt ctggaacttt
      241 ataactatca gcttgaaaat aactactctg ctatttcaca aatggcaata aacaaattag
      301 attatcctga tgaagtaaat cttaaacttg ctatagacaa actgcaagca gatagctatc
      361 tggcaattgc tgaagaaaaa caagtggagg aactgattaa cctatcaaaa aaaatagaaa
      421 aacagcagaa aaaaacaaaa tacaataatc ccgaactact ttcttctgcc tggtatgctc
      481 tttcctatta ttattttaga actgctgatt ataaaacagc taatttctat gctcataaag
      541 ctcaggattg gaattatcgc tttggcaatt ttccgggtat ggctcattgc ctatggctga
      601 aagctcaaat ttctgctgct gaaaataata tccctgcagc gaaaagctat tggaaaatgg
      661 cagaaggaat tttcagttct ctgcaggata atgtctctct ggaagaacta aaccatacta
      721 ggcaaaaaat gctgaaagaa caaaataaat gaaccagata ataatctaca ggaaaccaaa
      781 aaacggcttc aaacaaagac agaagtcagc tttggtgcaa atagaaataa attatgaaat
      841 tacttaagtc cgtttcccta cccacttctt caacagcact taaaacggtg aatgtactgt
      901 ttgcagataa aattatcaag atctccactg aggagattga agtagaggaa gatgttcaaa
      961 taattaatgc caagggttta ctaatgctcc ctggaggaat tgatccccat acccatattt
     1021 taacccaaaa tgctgattcg gcaactgact tggctaaaat aaccaaaatt gccttagaag
     1081 gaggttggac taccctttct gaactaagct atcatacaca aagtccggtt tttaataatt
     1141 cagctctcaa aaagaaaatt gctttggtaa atgccaattc ctatacctct ctggctcttt
     1201 ggggtcatat tgatattaac gattatcctt atcattcaga atcggcacag gaaatttgga
     1261 acaaaggagt tgtaggtatc gctttagctg ttccttctcc caatcctgcc attgcagata
     1321 ttactttccc ggaaataatg gatttgtttt ttgatatcta tgaaagcgat acatcctttg
     1381 ccttccaggg ttacgactat gaaagttatc gggaatttag tcctcttgct cagctggagg
     1441 caattaaaaa aatcttgcgt agaatgcagg aaaatcctat tcacattccc agaatttctt
     1501 cctatcctac aattgaattt attaacagta tttccaaaag aagtgatatt tcctatgcta
     1561 cttgtataat tgatctgatg gctcttttca atcctgaggt cttcagcact ccttttcttt
     1621 gtgactatgc tgattttgcc gatttacttt ttgaccttct gcgctccaat aaaatttacc
     1681 tgctttccaa ttgcgttgct cctccagtaa aaaatcccaa ttactgcgaa ctcttccagg
     1741 gttgcaatcc ggaactaatg aattattcat acctttgggt gctttccgaa ctctggaaaa
     1801 aaagaaaagt tccccttgcc acctgcctta aaatgaccag tgaaaatgcc gctaaacgtt
     1861 taggtatcta tcctcaaaaa gggtccttag accctggctc tgatgccgat tttgtccttt
     1921 acgatccgga atctacaacc ctcataaagg gtaataaaaa taaagatatt gaactctccg
     1981 gttcctttaa agctgtttat ttaaaaggaa atcaggttgc cggtaagaat tttgagacct
     2041 tacgccaggg ttctttttta ccccgtagca cgaatcctaa acgccgacat aataattctt
     2101 cctggattta gctttgaagt aaactacctt tctttacgaa aacaaaagaa cctgaaccta
     2161 tcctcttata ttgttttcat attaataatg cataaatatt tccctatttg atgtggattc
     2221 ctgcaaaatc agcaatccgg agatgtttat gcaatatatt tgccaaccca taaaaggaaa
     2281 taatgtcagt agagaaaaga aattggcgaa gaaagaattt gccttaaccg tattttttta
     2341 ttgcctgtcc attatttaga gcgtctaaaa ttataaattt attttaggtt gcaataattt
     2401 agcttgcaat ttacgagacc gttacctttt tgtagacagc gcattatttc ttgccaagtt
     2461 gaatctttta tgctgcatta acacttcttt aacgattcct taacaaagtt aaggcagtgt
     2521 tacaggatcg ttacggcagt gttaagggag cgttaaataa gtgattaggc aacaggcagt
     2581 tatacttgtg gtgtcctatt gtattcttcc taattttcta ttagcaaagg acgatataag
     2641 cattagttat ggcttttcaa taaaaatgct gtataatttc ctctaacttc cctattttgt
     2701 tataggtttc tgggcggttt tttctcacag ctgaaaaaat ccgcctttct taatgaaaac
     2761 catcaaactt tttttataat tccttacttt tatttatcgc tatatttacc aatgtgttac
     2821 ggatattatg aggataactg ataagataaa tatatcaata aaatgataat attgtaattt
     2881 tctccttgac aaaatatcag attaagaata taatgaaaac attaaaaacc aggaggttta
     2941 aaatgaaaaa aggattattt atccttctcg ccctaattgt cgcttcaagt gtttttgctt
     3001 ttacgaaagg aacgattaac ccgggaggaa cagtttcttt cacttctaac aaacctaatt
     3061 cagatgaaaa agccattact atgttcagca ttactcctca gattggttat tttgttatgg
     3121 ataacatgtc tgctgatgtg cttattaact atacttatga aggccaaagt gaaagtgatt
     3181 ataatgatga atatagctta tctaatttgg gcttaggaat tgggggaaga tatttctatc
     3241 ctttggaacc aggagaaatt tacggtggct tggattttct ttataattca tttagctggg
     3301 agagtggacc taatgatggt ggtaggaatg caatgtatct tggtttgaaa ggtggttatc
     3361 tttttccagt tgtagaaaat gtgtatgttg atttcgggct caaatatcaa atgggtttag
     3421 gtgattatgg tggtgatgat tttgaagatg tacctgatga atacaaggaa aatgaagaaa
     3481 gcacatttgg
//
LOCUS       DAOPFL010000133         3378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_38866_length_3378_cov_89.719531, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000133 DAOPFL010000000
VERSION     DAOPFL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38866_length_3378_cov_89.719531"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /gene="guaA"
                     /locus_tag="PLG69_07085"
     CDS             complement(<1..1502)
                     /gene="guaA"
                     /locus_tag="PLG69_07085"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPI43307.1"
                     /translation="MHNKVLILDFGSQYTQLIARKVRSLNIYCEIQPYQISLEQIQSF
                     APQAIILSGSPSSSSKINSPKPDEGIWQLRIPILGICYGMQLLVTHYGGTILPSNRSE
                     YGSAVLNIINHNELLAGLGDREEVWMSHSDAVSSLPECFSVLAKTDQSPYAVIAHKEL
                     PFYGLQFHPEVANTKNGIKVLENFLIRIGKITADWTPNAFINENIEKIRKQVGNQKVL
                     LGLSGGVDSSVTAALLYKAIGNQLLPIFVDTGLMRYREAERVKETFFAYPDLNIRYVD
                     ASKTFISKLKGITDPETKRKIIGNTFIEVFEKVAGEYEEVHFLAQGTLYPDVIESATF
                     TGMTAKIKSHHNVGGLPEKMNLALIEPLRELFKDEVREVGTLLGLPESIIQRHPFPGP
                     GLAVRIIGEVNAEKVRLLQLADEIFISELQKNGLYNTTWQAFAVLLPIRTVGVMGDER
                     SYDQVCALRAVDSIDGMTANFTQLPMWFLQKVANRICNEVKGISRVVYDIS"
     gene            complement(1511..2137)
                     /locus_tag="PLG69_07090"
     CDS             complement(1511..2137)
                     /locus_tag="PLG69_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPI43308.1"
                     /translation="MIKIESTVLLESFQTNTYLLWDSISAEAFLIDPAFPSETFLQRI
                     KQLELKVKMIINTHGHADHIGGNLYFAKALNCPVAIHLADAEMLTNNAKNFSTYMGFE
                     LNLSAPQVILHDGDELFLGSEKVKVIHTPGHTKGSICLLAGKYLISGDTLFEQSIGRT
                     DFPGGCHTAIIDSIKNKLFILDDDVLVFPGHGPTTSIGLEKVNNPFVN"
     gene            complement(2153..3148)
                     /locus_tag="PLG69_07095"
     CDS             complement(2153..3148)
                     /locus_tag="PLG69_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HPI43309.1"
                     /translation="MKTHKAIALFSGGLDSILAVKWMQSKGYAVHPVFFKAPYLLPER
                     AINYAAKNAIDLEVIDVSEEHIKLLDNPVYGFGKGLNPCIDCHGFMFKKAAELMPDRD
                     ADYLISGEVLGQRPKSQKRNALNNVSKLSGVKDLIVRPLSQNLLPDTLPLREGWIAKK
                     DMLSIQGRGRLAQLALAEELGIVDFPSPAGGCLLTDRNYCLRLQDLKKHQQDDLHSIN
                     LLAYGRHFRLTDEVKLIIGRDEAENETLKNIFREGLMLQAQDYMGPLGFITGKLNFAT
                     LDLALAIFILYHPKVPKSANIEVWRISNKQIIGQMIKTATKGDREIINKYLISYL"
BASE COUNT         1050 a          720 c          553 g         1055 t
ORIGIN      
        1 gaaatatcat aaaccaccct gctgatacct ttcacttcat tacaaatgcg attagcaact
       61 ttctgtaaaa accacattgg taactgagta aaattagcag tcataccatc aatactatct
      121 acagctctta aagcacaaac ctgatcataa ctgcgttcat cacccattac tccaaccgtt
      181 ctaataggta aaagaacggc aaatgcctgc caggttgtat tataaagtcc atttttttgt
      241 aattcactaa taaatatctc atccgcaagc tgaagcagac ggactttttc tgcatttact
      301 tctcctataa ttctaacggc taaacctggt ccaggaaagg gatgtctttg gataatggat
      361 tcaggtaaac caagcaaagt tcctacttct ctgacctcat ccttaaaaag ttctcttaag
      421 ggttcaatta aagctaaatt cattttttcc ggtaaaccac ctacattatg atgactttta
      481 atctttgctg tcattcccgt aaaagtggca ctttcaatta catcgggata gagagttccc
      541 tgtgctaaaa aatgcacctc ttcatattct ccggctactt tttcaaagac ctctataaag
      601 gtgttgccga ttattttacg ctttgtttcc ggatcggtaa ttccttttag tttggaaata
      661 aatgttttac tggcatccac atagcgaata tttaaatcag gataagcgaa aaaggtctct
      721 tttactcttt ctgcttctct gtaacgcatc aatccggtat ccacaaaaat aggcagtaac
      781 tgatttccga ttgccttata aagtaaagcg gcagtaactg aagaatcaac ccctccactt
      841 aaccccaaaa gaactttttg gttgcctacc tgttttctta ttttctcaat attttcattg
      901 ataaaggcat tcggagtcca gtccgcagtt atttttccaa ttctaatcag aaaattctct
      961 aatactttta ttccgttttt tgtgttcgct acttccggat gaaactgcaa gccatagaag
     1021 ggtaattcct tatgagctat tacagcataa ggggattgat ccgtttttgc taaaacagaa
     1081 aagcattcag gtaaactgga aacagcgtca ctatggctca tccagacctc ttccctatct
     1141 cccaatcctg caagtaattc attatggtta atgatattta atactgcaga gccatattcg
     1201 cttctgtttg aagggagaat agttccccca taatgagtta ccaataattg cattccataa
     1261 cagattccca aaatgggaat tctcagctgc caaattcctt catccggctt tggtgaattt
     1321 attttgcttg aactagatgg acttcctgaa agtataattg cttgcggagc aaaggattgt
     1381 atttgttcta aacttatctg atagggttga atctcgcagt agatgtttaa agaacgcact
     1441 tttcgggcta tcaattgcgt atattgagaa ccaaaatcta aaatcaaaac tttattatgc
     1501 attggtaaac ctaatttaca aaaggattat tgaccttttc caaaccaatg gaggtggtag
     1561 gaccatgacc cggaaaaacc aggacatcat catctaaaat aaagagctta tttttaatgg
     1621 aatcaattat agcagtatga catcctcccg gaaaatcagt tctgcctatg ctttgttcaa
     1681 ataaagtatc tccgctaatc agatattttc ctgccagcag acaaatactt cctttggtat
     1741 gacccggtgt atggataact tttacctttt cggaaccgag gaaaagctca tcaccatcgt
     1801 gcagaataac ttgaggagct gataaattta gctcaaagcc catataggta ctgaaatttt
     1861 tggcattatt ggtaagcatt tcagcatccg caagatgaat tgcaaccgga caattcaatg
     1921 ccttggcaaa atataaattt ccaccaatat gatctgcatg gccatgggtg ttaataatca
     1981 tttttacttt caattccaat tgtttaatcc gctgcagaaa tgtttcactc gggaaagcag
     2041 gatcaattaa aaatgcctct gcactaatgc tatcccaaag cagataagtg ttggtttgaa
     2101 agctctccag caaaacagtg ctttctattt ttatcatttt tgtatccctt tcttacaaat
     2161 atgaaatcag atatttattg attatctctc tatccccttt agtagcagtt tttatcatct
     2221 gaccgataat ttgtttattg ctaatcctcc aaacctcaat atttgcagat ttgggcactt
     2281 tgggatgata cagtataaag attgccagtg ccaaatctaa agtagcaaaa ttaagttttc
     2341 cggtaataaa tcctaaaggt cccatataat cctgtgcctg tagcatcaat ccttcccgaa
     2401 agatgttttt aagtgtttca ttttctgctt catctcttcc aataatcaat tttacctcat
     2461 ccgtcaagcg aaaatgacga ccataagcaa gtaagtttat tgagtgcaaa tcatcctgct
     2521 gatgcttttt caggtcttgt aagcgtaaac aataattacg atccgtaagt aaacaaccac
     2581 cagcaggaga aggaaaatca actattccta attcttctgc cagagctaat tgagccaaac
     2641 gaccacgacc ctgaatagat aacatatctt ttttagcaat ccaaccctct ctaagaggta
     2701 atgtatcggg aagtaaattt tgcgacaagg gccttacgat taagtccttt acaccgctaa
     2761 gtttacttac attattcaaa gcatttcgct tttgactttt aggacgctgc cctaaaacct
     2821 cgccactaat aagataatct gcatctctat ccggcatcag ttctgccgct tttttaaaca
     2881 taaaaccgtg acaatcaata caaggattta gtcctttacc aaaaccataa accggattat
     2941 ccaataattt tatatgttct tccgaaacat ctattacttc caaatcaata gcatttttag
     3001 cagcataatt aattgcccgt tccggaagca aatacggagc tttaaaaaat accggatgaa
     3061 cagcatatcc tttactttgc atccatttaa ctgccagaat actatccagt ccaccggaaa
     3121 ataatgctat tgctttatgt gttttcatat tgcctatgcc ctaaaactgc tcttttttgt
     3181 caagcaggaa atattcccaa aaggttgacc tcctggtcaa taaaaaagtc cggagtcaac
     3241 actgtgtagt cggaggacgc tgttcctccg caagcaagtg gaaaaggtga aacaaggagt
     3301 gggcattgta aaaaaaggat tcggagcttc ggcaagctcc tgctacacaa aaaaaacgag
     3361 ctccgaatac acaagatt
//
LOCUS       DAOPFL010000134         3294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_40233_length_3294_cov_112.331275, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000134 DAOPFL010000000
VERSION     DAOPFL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40233_length_3294_cov_112.331275"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PLG69_07100"
     CDS             complement(<1..659)
                     /locus_tag="PLG69_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPI43310.1"
                     /translation="MKTYKLLINNEHFEAKIVEYSPTHAKINLNGTDYLVQIEDDKIQ
                     SIPKLAAQEKAVPLAPAFSSSADLYSGEIRAPLPGVIVSILVSEGEEVKKGQAILIIE
                     AMKMQSEIASPVDGKIDKILVKERAPVKEGDLLMTITGAEIKVPPQSKPPQPSVEEPM
                     EKKAVTEKILRAPLPGTIMDIKVKVNDYVHEGDVVLVLEAMKMESDIYSPRTGKISKI
                     YV"
     gene            complement(663..1502)
                     /locus_tag="PLG69_07105"
     CDS             complement(663..1502)
                     /locus_tag="PLG69_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPI43311.1"
                     /translation="MRKIMFLTLFTFCVLSGWAQITSVEQTISDSLALAREKEIISEY
                     GFRDTATLSEVANKLQIEDIDRWKSYLGLEPHNEKLDKMSLRKLGITPYKALLAQQYS
                     IYGFTELSTLEEVAAALKMPVKKIKQEIGLDKPNSPKWDSYSLQSLNLTPEDIKKIHR
                     EFEEKSLNFGLNVTLVGMLTVFTALLITSIIIGQLNHLNRPAKEKKADMKITPGGKVR
                     SASATLNHNVIAALVTTIFLYEQSLEEKRKIALTFKRTPTNQWRASAMLNMPNREMYL
                     KRR"
     gene            complement(1513..3063)
                     /locus_tag="PLG69_07110"
     CDS             complement(1513..3063)
                     /locus_tag="PLG69_07110"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPI43312.1"
                     /translation="MENQSPLEKLRAKRAAALLGGGEKRIAEQHAKGKLTARERIALL
                     VDPDSFEEFDMFVTHRCTNFGLDKYIHPGDGVVTGCATINERMVYIFAQDFTVFGGSL
                     SKTYADKICKIMDMALKNGCPVIGLNDSGGARIQEGVDALAGYAEIFWRNVMASGVIP
                     QISAILGPCAGGAVYSPAITDFIVMEKSNSYMFVTGPKVVKTVLNETVTNEELGGAKV
                     HTSKSGVAHFIANSEEEAMLTIKLLLSYLPSNNLEDPPYVPSADPITRNCDVLNNLVP
                     ENPNKPYDMLDVIYPIVDDGEFLQVMSNFAQNIIVGFGRMNGRSVGIVANQPKILAGV
                     LDIDASIKGARFVRFCDSFNIPLVVLEDVPGFLPGTMQEHNGIIRNGAKLLYAFAEAT
                     VPKVTVIIRKAYGGAYCVLNSRHMRADIVYAWPSAEIAVMGPDGAVEVIFRKEAMAAE
                     NPKAVLQAREKEYREKFANPFIAAERGYIDDIIEPAETRFRLIRALEMLANKKDSIPP
                     RKHGNIPL"
BASE COUNT          926 a          702 c          585 g         1081 t
ORIGIN      
        1 acatatattt tgcttatttt ccctgttctg gggctgtaaa tatcggattc cattttcatc
       61 gcttccagaa ctaaaacaac atctccttcg tgcacatagt cattaacctt cacttttata
      121 tccattatgg ttccgggaag aggtgcacgc agaatttttt ccgtaactgc ttttttttcc
      181 attggttctt ctacgcttgg ttgcggtggc ttactttgag gaggaacttt aatttcagct
      241 ccggtgattg tcatcagtaa atctccttcc ttcacgggag ctcgttcttt cactaatatt
      301 ttatctattt taccgtcaac aggagaagca atttcactct gcatcttcat tgcctcaata
      361 atgagaattg cctgaccttt tttcacttct tcgccttcgg aaacaagaat agaaactatc
      421 actccgggaa gaggagcacg aatttctcca gaataaagat cggcactact gctgaatgca
      481 ggagccaaag gaactgcttt ttcctgggca gcaagcttgg gaatgctttg aattttatcg
      541 tcttctatct gaaccagata atccgttccg ttcaggttta ttttagcgtg ggtgggactg
      601 tattccacaa ttttggcttc aaaatgttca ttattaatta acaacttata ggttttcatt
      661 atttatctcc gcttaagata catttcccga ttgggcatat ttaacatagc gctggcacgc
      721 cattgattgg ttggtgtgcg tttaaaggtt aaggcaattt ttcttttttc ttccagggat
      781 tgttcataca aaaagatagt ggtaacaaga gcagctatta cattgtgatt taaagtggcg
      841 gaagcagaac gaacttttcc tccgggagta attttcatat ccgccttttt ttctttggca
      901 ggacggttca gatgattcaa ttgtccgatt ataatactag taataaggag agcggtaaat
      961 acagttaaca ttcctaccag tgttacatta agtccgaaat tcaaactttt ttcttcaaat
     1021 tcccggtgaa tttttttgat gtcttcaggg gttaagttca aggactgcaa agagtaagaa
     1081 tcccattttg gagaattggg cttatccagt ccgatttcct gtttgatttt tttcacgggc
     1141 attttaagtg cagcggcaac ttcttccaaa gtggaaagtt ccgtaaagcc ataaatgctg
     1201 tattgttgtg ccagaagtgc tttataaggt gttataccca gttttcttaa actcatttta
     1261 tccagttttt cgttatgcgg ttccaaacca agatagctct tccagcggtc tatatcctca
     1321 atttgcagtt tgttagctac ttcagaaaga gtagctgtat cccgaaaacc atattcggaa
     1381 ataatttctt tttcccgcgc taaagctaaa ctatcagata ttgtttgttc cacagaggtt
     1441 atttgagccc agccgcttaa gacgcagaag gtaaaaaggg ttagaaacat tatttttcgc
     1501 attactaata ccttacaaag gaatattgcc atgtttgcga ggaggaatgc tgtccttttt
     1561 atttgccagc atttctaaag ctctaattaa acgaaatctt gtttccgcgg gctcaattat
     1621 atcatcaata tatcctcttt ccgcagcaat aaaaggattg gcaaattttt cccgatattc
     1681 cttttctctt gcttgaagaa ctgccttagg attttctgca gccattgctt ctttacggaa
     1741 aataacttcc actgctccat caggacccat aactgcaatt tcagcagagg gccaggcata
     1801 aactatatcc gcacgcatat gacgactgtt taaaacacaa tatgcgcctc cgtatgcctt
     1861 acggataata acagtaactt ttggaactgt agcttccgca aaagcataaa gaagttttgc
     1921 cccgttacga ataattccat tatgctcttg cattgtgccg ggtaaaaaac cgggaacatc
     1981 ttccaaaact accaaaggta tattgaaaga atcacaaaat cgcacaaaac gagctccttt
     2041 aatactggca tcaatatcta aaacaccggc taaaattttg ggctgattag ctacaattcc
     2101 tacactacgt ccattcatcc tgccaaaacc gacaataata ttctgggcaa aattactcat
     2161 cacttgcaaa aattcgccat catcaacaat cggataaatc acatccagca tatcgtaggg
     2221 cttattagga ttttcaggaa caagattatt caaaacatca caattacgag ttataggatc
     2281 agcactcgga acataaggtg ggtcttccaa attattggaa ggcaagtaac ttagcagaag
     2341 tttgatagtg agcattgctt cttcttcgct attagctatg aaatgagcta caccgctttt
     2401 gctggtatga acttttgcgc cacctaattc ctcattagta accgtttcat ttaatacggt
     2461 tttaacaacc ttaggaccag taacgaacat atagctgtta cttttttcca taacaataaa
     2521 atccgttatg gcaggtgaat atacagctcc gcctgcacag ggacctaaaa ttgccgaaat
     2581 ttgaggtatc actccgcttg ccattacatt acgccaaaaa atttccgcgt aaccagcaag
     2641 agcatcaacg ccttcctgaa ttcttgctcc accggaatca ttcagtccaa taacaggaca
     2701 accgttcttt agtgccatat ccattatttt gcagatttta tcagcgtagg tcttggataa
     2761 agaaccacca aaaaccgtaa aatcctgggc aaaaatatag accattcttt cattgattgt
     2821 tgcgcaaccc gttaccacac catctcccgg atgaatatat ttatccaatc caaaatttgt
     2881 gcaacgatgg gttacgaaca tatcaaattc ttcaaaacta tcaggatcaa ccaaaagggc
     2941 aatcctttct cttgcagtga gtttaccttt tgcgtgttgt tcagcgatgc gtttttctcc
     3001 accgcctaaa agtgctgcag cgcgttttgc tctcagcttt tccagagggc tctggttttc
     3061 catttctttt ctttcctgtt ttttgcttat tttttcaaaa gtaatgccat agtcattttt
     3121 tctaaagcgg ttttttagta aagggaaatg ttctcttgtt gttaccctgt aaaaggttaa
     3181 aattgcctct ttttcttcta ctgtaactgc ttatacaatc aataggatta ctgaattcct
     3241 atccggcaaa ttaataggta taatttatct atagctatga tgtttcttgt tgcc
//
LOCUS       DAOPFL010000135         3256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_40858_length_3256_cov_96.216807, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000135 DAOPFL010000000
VERSION     DAOPFL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40858_length_3256_cov_96.216807"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="PLG69_07115"
     CDS             <1..1715
                     /locus_tag="PLG69_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI43313.1"
                     /translation="FQEYVLRKQYGNTSFVQRRGNFFKDQLNYHFLRYFGMQWLNEPF
                     FTKPLGVTSALLKPIANIFIAFLGIVGAMLQFRKNKHSFWYFFSVILITTVVMVFVMN
                     LSDAEVRDRDYFFVVAYNMWAIWMGIGALALVTLWKSKPVRVVLLIFMCLLPVSNLIS
                     QYRVHDRSQEFIALDYGLNFLNSVEENAIIFTNGDNDTFPLWYAQAVEDPYSKENIYP
                     ARDVYPTKESKEAIAKAMEYKNKCLKGIRKDVSVANLSLLNTSWYIRQLRDQEGILFN
                     IPDDQLDELEPRRIEEPLVIPGPPDNPSMGFTMDIPPTAEWRTNEPFYRVSDLAVMQI
                     VKDNYGKRPIYFAVTCESYVNFNDYVRNEGMVGRIVSTPKEEQIDPERLLHNIDVVYN
                     YRSIEDPRVYKDDNMRRLVMNYGSGFVRAANYYIDTGNYPKALEYINKAKKFVDDEIK
                     LTEFYTNYYSKTGQWNKLDEFINRNLATHPEGWKIYLSYIIIHLVDNYPDKIITYLKK
                     GFLLFPDQEYFAQFAVSYAQEYKEYDVIHKLLEEVAPKLQYDISGYQSDLEELSKQKI
                     SEYM"
     gene            1814..2611
                     /locus_tag="PLG69_07120"
     CDS             1814..2611
                     /locus_tag="PLG69_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPI43314.1"
                     /translation="MNKKSNRQALRHIIQLLFLLLSLAFFAGIIFFSLPYTIHTICPY
                     AIVCFGLSKNILIQYLGNPFAITIAFSFLILVLTIFGGRLFCAYICPLGTLQEIIYAL
                     FGKKRKNRKQIPQFQERRFAYVKYIVLVVTIILSLIGVNYIFIHLCPVYALSLLPDIA
                     VWGLIVTLLIIVAGIFLERFWCRFLCPYAALMNLFQHLGQLVHFPRLKIHRNLERCND
                     CELCSLSCPMNINITENESVQNPNCILCFKCIKKCPKPETLKGKMEK"
     gene            2612..3166
                     /locus_tag="PLG69_07125"
     CDS             2612..3166
                     /locus_tag="PLG69_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPI43315.1"
                     /translation="MKVILQILVLLLLGGMLIFLFYNAFLSFAMNKDDCSVTSIPQKE
                     SRQFNKHTLNSDRQDKISTPKENEFSFTIRTQSQNIKTEPSSTQTQAKTSLSELPLEN
                     KLLSTEPSETTNPSPKTIYQVNKDLCIGCKLCIRYCPEGAIYIKNGKAVIDTTKCTAC
                     GICSDGNNKNFTGCPVGAVFSGKL"
BASE COUNT         1003 a          603 c          601 g         1049 t
ORIGIN      
        1 tgtttcagga atatgtttta agaaaacaat acggtaatac gagttttgtg caaaggcggg
       61 gaaatttctt taaagaccag ttaaattatc actttttacg ctattttgga atgcaatggt
      121 taaatgagcc atttttcaca aaacccttag gtgtaacttc cgctctgttg aaacccatag
      181 ctaatatttt tattgccttt ttgggaatag tcggcgctat gttacaattt cgcaaaaata
      241 aacacagttt ttggtatttc ttctcggtaa tcttaattac tacagtagta atggtttttg
      301 taatgaatct ttccgatgcc gaagttcgtg atcgtgatta cttttttgta gttgcctata
      361 atatgtgggc aatctggatg ggaataggag ctttggcatt ggttacactg tggaaaagta
      421 aacctgtaag agttgtttta ttaattttta tgtgcctttt gcccgttagc aatttgatct
      481 ctcaatatcg ggttcacgat cgttctcagg aatttatcgc tctggattat ggattgaatt
      541 tccttaattc ggtggaagaa aatgccatca ttttcaccaa tggagataat gacacttttc
      601 ctctctggta tgcacaagcg gttgaagacc cttattccaa agagaatatc tatcctgcca
      661 gagatgttta tcctacaaag gaaagtaaag aagcaatcgc taaggcaatg gaatataaaa
      721 ataaatgcct gaagggtatc cgaaaagatg tttccgttgc caatctttcc cttttaaaca
      781 ccagttggta tatcagacaa cttagagacc aggaaggaat tctgtttaat attcctgatg
      841 accaattgga tgaattggaa ccaagaagaa ttgaagaacc gttggttatt ccgggaccac
      901 cggataatcc ttcaatgggt tttacaatgg atatacctcc tactgccgaa tggagaacaa
      961 atgaaccttt ctatcgtgtt tccgaccttg ccgtgatgca aattgtgaaa gataattatg
     1021 gcaaacgacc tatttatttt gccgtaacct gcgaaagcta tgttaatttt aatgattatg
     1081 ttcgtaatga aggtatggtt ggtagaattg tttccactcc caaagaagaa caaatagacc
     1141 cggaacgctt gttacataat attgatgtgg tttataacta ccgctccata gaagacccaa
     1201 gagtatataa agatgataat atgagacgac tcgtaatgaa ttacggttcc ggttttgtgc
     1261 gtgccgccaa ctattatata gataccggaa attatcctaa ggccttggaa tatattaata
     1321 aagcgaagaa atttgtggat gatgaaataa aactgaccga attctatacc aactattaca
     1381 gcaaaacagg acagtggaac aaactggatg aatttatcaa tcgcaatctc gctactcatc
     1441 ccgagggctg gaagatttat ttaagctata ttattataca tttggtagat aattatcccg
     1501 ataaaatcat cacctacctc aagaaagggt tcctgctttt ccctgatcag gaatattttg
     1561 cccaatttgc tgttagttat gctcaagaat ataaagaata tgatgtaatt cataaacttc
     1621 tggaggaagt agctcctaaa cttcaatatg atataagtgg atatcaatca gacctggaag
     1681 aactctctaa acaaaaaata agtgaatata tgtagaatga attaagtcag aagtcgtcac
     1741 tgccgaaata actttaaccc tatctgaaaa ctattttggc agtgaggact aatgacgacc
     1801 aagaaagacc aaaatgaata aaaaaagcaa cagacaagcc ctcaggcata ttatccaact
     1861 gctcttttta ttgttatcac tggctttttt tgcaggtatt atcttcttta gcttgcctta
     1921 cactattcat actatttgtc cttatgctat agtttgtttc ggcttaagca aaaacatcct
     1981 tattcaatat cttggcaacc ctttcgcgat aacgattgcc ttcagctttt taattctggt
     2041 tttaacgatt tttggcgggc gccttttctg tgcctatatt tgccctctgg gaactttgca
     2101 ggaaattatc tatgcccttt tcggcaaaaa gcgaaaaaac agaaaacaaa tccctcaatt
     2161 tcaagaaagg cgctttgctt atgttaaata tatagtgttg gttgtaacta tcatcctttc
     2221 tctaatagga gtaaactata tttttatcca tttatgccct gtttatgctc tttccctttt
     2281 gccagatata gctgtttggg gtttgattgt aactctcttg ataattgtgg cgggaatttt
     2341 tctggaacgc ttttggtgtc gttttctttg tccttacgct gctttaatga atttgtttca
     2401 gcacctggga caacttgtcc attttccccg cttaaaaatt caccgtaatc tggaacgctg
     2461 taatgattgc gaactttgct ctctatcttg tccgatgaat attaatatca cggaaaatga
     2521 atctgtgcag aacccgaatt gcattctttg ttttaagtgc ataaaaaaat gccctaagcc
     2581 cgaaaccctg aaaggaaaaa tggaaaaata gatgaaagtt attctgcaaa tattggtctt
     2641 actgcttttg ggaggtatgc ttattttcct gttttataat gctttcctat cttttgctat
     2701 gaataaagat gactgctctg taacttcaat tccgcaaaag gaaagcaggc aatttaataa
     2761 gcatacccta aactctgatc gccaggataa aatttcaacc cctaaagaaa atgaattctc
     2821 atttacaata agaactcagt cccaaaatat taaaactgaa ccatcttcta ctcaaacgca
     2881 agctaaaact tccctctccg aacttcctct tgagaataaa cttttgtcta ctgaaccatc
     2941 agaaacaacc aatccctccc ctaaaactat ctaccaagta aataaagatc tctgtattgg
     3001 ctgtaaactc tgtatccgct attgtcctga aggtgctatc tatattaaaa acggaaaagc
     3061 tgtaattgat actacaaaat gcactgcttg tggaatctgc tctgatggaa acaataaaaa
     3121 ctttacgggt tgcccggttg gcgctgtgtt ttcaggaaag ttatgaagtg ctggagtgca
     3181 ggagtgctgg agtgctggag tgctgaagtt agtgaaaaag tgaaacatgt tctgaacttc
     3241 ccccagatat tcttgt
//
LOCUS       DAOPFL010000136         3236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_41202_length_3236_cov_115.251179, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000136 DAOPFL010000000
VERSION     DAOPFL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41202_length_3236_cov_115.251179"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /gene="queD"
                     /locus_tag="PLG69_07130"
     CDS             <1..357
                     /gene="queD"
                     /locus_tag="PLG69_07130"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPI43316.1"
                     /translation="TFSAAHLLRGYEGACSKLHGHNWKVRVCVKTKEQDEIGMAMDFG
                     VLKTILSNILNNLDHSYLNEIVPFTERNPTSENLAKYIYECIEKELQDKPVAISEVEV
                     YESEKSSVIYKNDQIC"
     gene            341..952
                     /gene="yihA"
                     /locus_tag="PLG69_07135"
     CDS             341..952
                     /gene="yihA"
                     /locus_tag="PLG69_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPI43317.1"
                     /translation="MIRFVESFFVKSAIEPSDYPASAYPEFAFAGRSNVGKSTLINLL
                     TNHKGLAKTSSTPGKTRLLNFFLIRYKIDDNDSSGFLQFTDLPGYGFAEVSQAEQEKW
                     RRMINKYFEQRKQLRSLIVIVDIRHPADSKDIMLLEMLQLRGIDYCIVATKADKIPKT
                     KVAKTVKDLAKDLGLKEEPIFPVSALNNTGLEPLYNWFQNKLS"
     gene            954..2801
                     /gene="mnmG"
                     /locus_tag="PLG69_07140"
     CDS             954..2801
                     /gene="mnmG"
                     /locus_tag="PLG69_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPI43318.1"
                     /translation="MHTDNKFDLIVVGAGHSGIEASLAAAKRGLKVALFTIKIEAIGR
                     MSCNPSIGGPAKGHLAREIDALGGELAKSADLSGIHFRMLNRSKGPAVWAPRSQNDRQ
                     KYHLLMREAIEKEENIHLIEATIEEIIVENGCVKGVISQIGHCYYAPKVILATGTFLQ
                     GKIHIGKISYAGGRSGEPSVEYLSRSLSAWGLKVRRFKTGTPPRVDLRSVNYNKLEEQ
                     KGDENPQGFSFYRDIELKNLVSCYITHTTEETHQIIRANLTESALYSGIIKGIGPRYC
                     PSIEDKIVKFPQRESHHIFIEPEGLNTFEGYVNGISTSLPAEIQEKIVHSIPGLEEAR
                     ILRYAYAIEYDFIDPEEIDSSLQCKKIKGLYLAGQINGTSGYEEAAAQGLLAGINASL
                     ALENKEPLILKRSEAYIGVLIDDLVTRGTNEPYRMFTSRAEYRLLLRQDNADERLMPV
                     GYKLGLVEESRWQRFLQMQEILQRELEYLKQHNCLPNGEIKEPIRFAQLLKRPEISFN
                     DLQNYGYKIPADVNSDIQNRLELEIKYSGYLNRMEEEIQKFQNAETIAIPEEMDYFSI
                     PSLAYEAREKLSKIRPKNIGQAMRIPGINYSDSVALMIWLRKNAKKEAK"
     gene            2798..>3236
                     /locus_tag="PLG69_07145"
     CDS             2798..>3236
                     /locus_tag="PLG69_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPI43319.1"
                     /translation="MNIIALIPARYNSTRFPGKPLALLKGKPIIQYVYERVANSGLFN
                     TVCVATDNISILETVKSFGGKAVLTKESHPSGSDRIAEALQYFPQAELVFNVQGDEPL
                     IEKEPLQKLISAFEDRKVQMASLMTVIEDREMIYNPNIVKVVTD"
BASE COUNT         1055 a          574 c          696 g          911 t
ORIGIN      
        1 accttttccg cagctcattt attgcgtggt tatgaaggtg cttgcagtaa actgcacggt
       61 cacaactgga aggtgagggt ttgtgtgaag acaaaagagc aggatgaaat cggaatggca
      121 atggatttcg gtgtactcaa aactattctg tctaacatac taaataacct ggatcattct
      181 tatttgaatg agattgttcc gtttacggaa agaaatccca cttccgaaaa tctggctaaa
      241 tacatttatg aatgtattga aaaagagctt caggataagc ccgtagctat ctctgaagta
      301 gaggtttacg aatccgaaaa aagcagtgtg atttataaaa atgatcagat ttgttgagtc
      361 cttttttgtt aaaagtgcta ttgaaccgag tgattatccg gcttctgctt atcccgaatt
      421 tgcttttgcg ggtagaagca atgtaggtaa atccacactt atcaatttgt taaccaatca
      481 taaagggctt gctaaaacat catccactcc tggaaagaca cgcttattga atttcttttt
      541 aattcgttat aagatagacg ataatgatag ttccggcttt ttgcaattta ctgatttacc
      601 aggttacggc tttgcggaag ttagccaggc agaacaggaa aaatggcgtc ggatgatcaa
      661 taaatatttt gaacagcgca agcaattgcg ttctctaatt gttattgtag atattcgtca
      721 tccggcagac agcaaagata ttatgctatt ggaaatgtta caattaagag gtattgacta
      781 ttgtatcgta gctaccaagg cagataaaat tcccaaaacc aaagtagcca agacggtgaa
      841 agacctggca aaggatttgg gcttgaaaga agagccgatt tttcctgttt cagcacttaa
      901 taatacaggt cttgagcccc tttataactg gtttcaaaat aaattaagct aaaatgcata
      961 cggataacaa atttgatctc atcgttgtgg gagcaggtca ttccggaatt gaggcatccc
     1021 tggcagcagc aaaaagaggg ttgaaagttg ctctctttac cattaaaatt gaagctatag
     1081 gcagaatgag ctgcaatcca tccatcggag gtcctgctaa aggtcatctg gcaagagaaa
     1141 tagatgcctt gggtggtgaa ctggcaaaaa gtgccgacct ttccggaatt cattttcgga
     1201 tgctaaatag aagtaaaggt ccagctgttt gggctccacg ttctcaaaat gatcgtcaaa
     1261 aataccatct gctgatgcga gaagcaatag aaaaggaaga aaatattcat ttaattgagg
     1321 caaccataga agaaataatt gtagaaaatg gttgtgttaa aggagtaata agccaaatcg
     1381 gacattgcta ttatgctcct aaagttattt tagctaccgg cacttttttg cagggaaaaa
     1441 tccacattgg caaaataagt tatgccggag gacgcagtgg agagccatct gtagaatatc
     1501 tttcccgttc tttatctgct tggggattaa aagttcgcag gtttaaaacg ggaaccccac
     1561 ccagagtgga cttgcgttct gtaaattata acaaactgga agaacaaaaa ggggatgaaa
     1621 atcctcaggg cttttctttt taccgggata ttgaacttaa gaatctggtt tcttgttata
     1681 taacacacac tacggaagaa acgcatcaaa ttattagagc caatttaact gaatccgctc
     1741 tttattccgg aataattaaa ggcatcggac cgcgttactg tccttctatt gaggataaaa
     1801 tagtgaaatt tccccaaagg gaaagtcatc acatttttat tgaaccggaa gggctgaata
     1861 cctttgaagg ttatgtaaac ggcatttcta cttcgcttcc ggcagaaatt caggaaaaaa
     1921 ttgtgcacag tattccgggt ttggaggagg cgcgtatttt acgctatgct tatgctatag
     1981 agtatgattt tattgatccg gaagaaatag atagttctct acagtgtaaa aaaataaagg
     2041 gcttatacct ggcaggacag ataaatggca cttccggtta tgaagaggca gcggcacaag
     2101 gtcttttagc aggtataaat gcttctctgg ctttggaaaa taaagaaccc cttattctaa
     2161 aacgcagtga ggcatacatt ggggttctaa ttgacgatct tgtaacccgg ggaacaaatg
     2221 agccctatag aatgtttact tcgcgtgcag aataccgttt acttttacgt caggataatg
     2281 ctgatgagcg tttaatgcca gttggttaca aattgggatt ggtagaagaa agtcgttggc
     2341 aaaggttttt gcagatgcaa gaaattttac agcgggaact ggaatacctg aaacagcata
     2401 actgcctgcc aaatggagaa ataaaagagc ccatccgttt tgctcaatta ttgaaaagac
     2461 ccgaaatttc ttttaatgac ctccaaaatt acggttacaa aattccggcg gatgttaata
     2521 gtgatattca aaatcgcctt gaactggaaa ttaaatattc cggctattta aaccgaatgg
     2581 aagaagaaat tcaaaagttc caaaatgccg aaacaattgc cattccagaa gagatggact
     2641 attttagcat tccctctctg gcttatgaag cacgcgaaaa actaagtaaa atccgtccta
     2701 aaaatatagg gcaggcaatg cgaattccgg gaataaatta tagtgatagc gttgccctaa
     2761 tgatttggtt gcgcaaaaat gccaaaaagg aagccaaatg aatattattg ccttaattcc
     2821 agctcgttat aattcaacgc gttttcccgg aaaaccctta gcactgctga aaggaaaacc
     2881 gattatccag tatgtatatg aacgcgttgc aaattccggc ttatttaata ctgtttgtgt
     2941 agcaacggat aatatctcca ttttagaaac agttaagtca ttcggaggca aagcggtttt
     3001 aacaaaagaa agtcatccca gcggttcaga ccgcatagct gaagcgctac aatattttcc
     3061 gcaggcagaa ttggttttta atgttcaggg agatgaaccg cttattgaaa aagaaccctt
     3121 acaaaagctt atttccgctt ttgaagatag aaaagtgcag atggcaagtt taatgaccgt
     3181 gattgaagac cgggaaatga tatacaatcc aaacattgta aaagttgtaa ctgata
//
LOCUS       DAOPFL010000137         3202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_41821_length_3202_cov_73.092151, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000137 DAOPFL010000000
VERSION     DAOPFL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41821_length_3202_cov_73.092151"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(616..2898)
                     /locus_tag="PLG69_07150"
     CDS             complement(616..2898)
                     /locus_tag="PLG69_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPI43320.1"
                     /translation="MRLNRNQLARKIMLVAGLCLVTIIMWAKVVEPENALLAAKNYLQ
                     EAGLKSPIYAGNAELFVYSSGKLLPLEFNKAVEFEPTLYFINYANGNYAIVSAEDNFY
                     PVLAYSDEGLTNINNLPPAFYYWLENYSAQIEQIREAKITYPENVQLWQSLIEGSYRN
                     AFRTERSVRPLVTTLWDQGWPYNALCPVDAQGSGGHVYAGCVATAMGMVMKYWNHPQT
                     GVGSESYYCPGYGYQSANFGNTTYLWDQMYDTAGATPAEYLPIATLLYHCGVAVHMAY
                     SVEGSGAQSTDAAVAFVDHFRYPNAQYVMKNSYTDANWNNLLTSQIDNGIPVYYSGYD
                     PVEGGHAFVMDGYDTANHFHFNFGWSGSGNGYFYTSNPGGFTNNQSAIINIIPENYSI
                     STVPVKLNAHDTTAGDNFTVTVKTNPILGSWNITHYDFVLYYDSEFIDYIGYSTTGTI
                     SENGTITVVENPAGIISVDWNSTNYIFGGGVLVNFTFRTRDMGDFLFDITSMHYNTTP
                     VSNISYVMIHSYAPVNNIAESRILLTNIMNLAYNAIGTTQMNTTYLLPSWNITHFQYH
                     LNYNPAKIEYFDIVTEGTISANCEVNVDSSNPGVLNISGNSTVPLIGAGALMKIRFKA
                     IGNTGSISVTQISISDFLYNNVAISDVGTANVILSAYTANEDEIVAVPEPKLEIYPNP
                     FQDSAMLKFTGTNKAPVRIHIYNIKGQLVKELLISDPLNSQISWNRSDVKGKTVADGI
                     YFLHWQQGEQSGINKVLVIK"
BASE COUNT         1039 a          626 c          498 g         1039 t
ORIGIN      
        1 tgcagaaagg gaaatacggg atttcctggc aaaaaacgaa aaggatactt tttttcataa
       61 agagaaaaag agaataatta agaatgaaga atgatagcat gcggattacg cagatttttt
      121 acaaagagaa aaagagaaga agagaaaagt gagatggtga gctggattat ttccacaaaa
      181 gagaaaaaga gaaaagtaaa atggtgagat ggattattcc cacaaagaga aaagttatat
      241 catttttttg aatgaaatat cttttgtcat tcctacgcaa gcaggaatcc atacaagttc
      301 tgataaatta actgcatttt aaatacaaaa cttaataata ctttatctat aatcataaat
      361 ggcagaaaaa gtccgtaaaa acgacagata ttgccttatc tatttaaatt tcaaacttcc
      421 tgtctgatga tttttcctgc cttttttcgg ataaattgtc tatctctatt ccctttagat
      481 gttgagaaag tttgctacaa atacgcaatt tcttcaccaa aggattgaaa ctctttttct
      541 atataaaaag atgactcctg atattttgct aatcaggagt catcaaatct ttttttactg
      601 aattacgctt ttatcttact taattaccaa tactttatta atgccgcttt gttctccctg
      661 ctgccaatgc aggaaataaa ttccatccgc tacagttttt ccctttacat cacttctatt
      721 ccaactgatc tgagaattca acggatcgct tatcagcaat tcctttacta attgaccttt
      781 aatattgtaa atgtgaattc taacaggagc tttgttggtt cctgtaaatt ttagcattgc
      841 actatcctga aaaggattag gatagatttc cagtttcggt tcgggaacag caacaatttc
      901 atcttcatta gctgtataag ctgaaagtat cacattggca gttccaacat ctgaaattgc
      961 tacattatta tacaagaaat cactaataga aatctgagtg acggatatag aacctgtatt
     1021 acctatggct ttgaatctaa ttttcattag agctccggct ccaattagcg gaacagttga
     1081 attacctgaa atatttaaaa cacctggatt ggatgaatca acattaactt cacaatttgc
     1141 acttatcgtt ccttctgtta cgatgtcaaa gtattctatt ttggcaggat tatagttaag
     1201 atgatattga aaatgtgtaa tattccagga aggaagcaaa taagtagtat tcatctgtgt
     1261 agtaccaatt gcattataag ccagattcat aatattggta agcaaaattc ggctctcagc
     1321 tatgttgttt acaggagcat aagaatgaat catcacatag cttatatttg aaaccggtgt
     1381 agtattataa tgcatactcg ttatatcaaa cagaaaatct cccatatctc tggttctaaa
     1441 tgtaaaatta accaatactc caccaccgaa gatataattc gtactattcc aatctacgct
     1501 tataattccg gcaggatttt caactacggt aatagttcca ttttcagaaa tagttcccgt
     1561 ggtggaataa cctatataat caataaattc actgtcataa tataacacga aatcgtaatg
     1621 tgttatattc cagctaccca gaatgggatt cgttttaacg gtaacagtaa aattatcgcc
     1681 cgcagttgtg tcatgcgcat taagttttac cggaacagta gaaatgctgt agttctccgg
     1741 aataatattg ataatagcac tctgattgtt agtaaaacca cctggattgc tggtataaaa
     1801 ataaccatta cctgaaccac tccaaccaaa attgaaatgg aaatgattag cagtatcgta
     1861 accatccatt acaaaagcat gacccccttc tacgggatca taaccactat aataaacagg
     1921 aatgccgtta tcaatttgag aagttaataa attattccaa ttggcatcag tataactgtt
     1981 tttcattaca tattgtgcat taggataacg gaaatgatct acaaatgcca cagctgcatc
     2041 tgtactttga gctcctgaac cttcaacaga atatgccata tgaacagcaa caccacaatg
     2101 atataataat gtcgcaatcg gcaaatattc agctggtgtc gctcctgctg tatcatacat
     2161 ttgatcccaa aggtaagtag tgttcccgaa attagcactt tgatagccat aaccgggaca
     2221 atagtaactt tcactaccta ctccggtttg gggatgattc caatatttca taaccatacc
     2281 cattgctgta gctacacaac ctgcatatac atgacctcca gaaccttgag catctactgg
     2341 acaaagcgca ttatagggcc atccttgatc ccaaagcgtc gtcactaaag gtcttacaga
     2401 acgctctgtt ctaaaagcat tcctataaga accttcaatt aagctctgcc acagttgaac
     2461 attttccgga tatgtaattt tcgcttctct aatttgttca atttgagccg aataattttc
     2521 caaccagtaa tagaaagcag gtggcaggtt attgatattg gttaaccctt catctgaata
     2581 tgctaaaaca ggatagaaat tatcctcggc ggacacaata gcataattac cgtttgcata
     2641 attaataaaa tagagtgttg gctcaaattc caccgcttta ttgaactcca aaggcaatag
     2701 cttccccgaa ctataaacaa atagttcggc attacctgca taaatcggac tttttagtcc
     2761 cgcttcttga aggtaatttt ttgcagccag caaagcattc tccggctcta ctactttagc
     2821 ccacataata atagtgacca ggcataatcc tgcaacgagc ataatttttc tcgctaattg
     2881 attccgattt aatctcattt atcctcccgg atatatatta tttatttata tatgttattt
     2941 tatgcacgaa ttattccatc cctctcctta ttcctttact ttaacttagt tctttactct
     3001 ctttaagttt ttttatgtcc gctttttctc acttacttct attaccatcg tttatctgat
     3061 tacctgtata tatttcttta tcctatttat acaataacgc gattatctta tgcaggcaaa
     3121 ccttaatatt tttattgcct tttaaactct ctacttctcg acttcaaaaa attcgccgtt
     3181 agcgatggcg gcgacctact ct
//
LOCUS       DAOPFL010000138         3169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_42418_length_3169_cov_115.639371, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000138 DAOPFL010000000
VERSION     DAOPFL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42418_length_3169_cov_115.639371"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1013)
                     /locus_tag="PLG69_07155"
     CDS             complement(129..1013)
                     /locus_tag="PLG69_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPI43321.1"
                     /translation="MFTQSVSDILKKIRRIEIRTRNPVAEIFRGEYHSRFKGQGLEFS
                     EVREYQPGDNYRDIDWNVSARLGQLYIKKYRETRELRVVFLVDVSASQEFGTRSMIKR
                     ERIAELVAALAFSAVANQDLAGLILFSDRVEKFLPPRKGRNNALAILREVLYYEPKSK
                     KTSLQVACEYAHKMLKKRCILFIISDWIDSGYEHSLKILATKNDVIAIRVLDDSELEL
                     PRAGILSLQDPETGEEVWINSSDSKLRKAYQEIVNKEQKDLEEFFRKCNCDYLLIRTS
                     ESYINSLRKFFTLRERRR"
     gene            complement(1027..2013)
                     /locus_tag="PLG69_07160"
     CDS             complement(1027..2013)
                     /locus_tag="PLG69_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPI43322.1"
                     /translation="MLIEAIQNEVEKSSAVLGNVRSEIARVIVGQQEIIDRLLIGIIA
                     NGHILIEGVPGLAKTLIISSLASVFDASYSRIQFTPDLLPADITGTMIYNPKSGDFSV
                     KKGPVFANFILADEINRAPSKVQSALLEAMQERQVTLGDNTFPLPNPFFVMATQNPIE
                     QEGTYPLPEAQIDRFFMKLKIKYPAYDEERQILERMVNEQPIPLQKVLSPKDIEEMRE
                     VMHKVYMEEKLKDYILHLVNSTRHPERYPRLNDLKGLIEFGASPRATIFLARAAKTNA
                     YISGRAYVIPDDIKMIAKDVLRHRIILSYEAEAEELTSEDIINRILDEIEVP"
     gene            complement(2162..2353)
                     /gene="rpmB"
                     /locus_tag="PLG69_07165"
     CDS             complement(2162..2353)
                     /gene="rpmB"
                     /locus_tag="PLG69_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPI43323.1"
                     /translation="MSKVCDICGKTAQVGNHRSHALNATKRRFYPNLHEIRVAGDGGT
                     KKIKVCSSCLKANKVRKAV"
     gene            complement(2420..>3169)
                     /locus_tag="PLG69_07170"
     CDS             complement(2420..>3169)
                     /locus_tag="PLG69_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPI43324.1"
                     /translation="IMLNLTKVISSRLRNTNERFVELMDEMLKKNRLMAIGLAASKII
                     HDIKTPLTVIVLTAQLLENIFPESAEFTDNIVKQTKLIDQLVHEILDFARGTESPPLI
                     QKVNLDSFLQEISELYSASLKERKINFVVENKVNDCVYFDEGKIRRVLINLIKNALEA
                     ISEAGEIKIISSVSSGWLQISVIDNGPGVSQKVKEELFQPFVTDGKTQGTGLGLAICK
                     KLVQEHHGRLEYIPVEPHGSRFDIRIPQNTK"
BASE COUNT          940 a          693 c          546 g          990 t
ORIGIN      
        1 agtaatttta aagagaaaag gagtgcctac ttttaaacta tcggcaccct gaagttcctg
       61 acttaattca gcccggataa ataagggaag caataaaaac agaaaagaca caattacttt
      121 tattcttttc atcttctcct ttcccttaaa gtgaagaact ttcttaagga attgatatag
      181 gactcggaag tgcggataag caaatagtca caattgcatt tacgaaagaa ttcttccaag
      241 tccttctgtt ctttatttac tatttcctga tatgctttac gcagtttgga atcggaactg
      301 tttatccaaa cttcctctcc tgtttccggg tcttgcaaag ataaaatgcc tgcccgggga
      361 agttccagtt cgctatcatc caaaactctt atagcaatta catcattctt ggtagccaga
      421 attttgaggg aatgttcata accagaatct atccaatccg aaataataaa cagaatacag
      481 cgttttttca gcattttatg ggcatattca caggcaacct gcaaagaggt ctttttgctt
      541 ttgggttcat aatagagaac ttctctgagg attgccaaag cgttattccg tcccttacgg
      601 ggtggcagaa acttttccac tctgtccgaa aaaaggataa ggccagccaa atcctgatta
      661 gcaacggctg aaaaagccaa agctgcaacc agttctgcaa tcctttctct tttaatcata
      721 ctgcgagtgc caaattcctg tgaagcacta acatccacta agaaaaccac cctcaactct
      781 ctggtttccc gatatttttt aatgtaaagt tgtcccaaac gcgcagaaac attccagtca
      841 atatctctgt aattatctcc cggttgatat tcacgcacct cagaaaattc caaaccctga
      901 cctttgaacc gactgtgata ttctcctctg aatatttcag cgacgggatt gcgagtgcga
      961 atttctatcc tgcgaatctt tttcaggata tcagaaacgc tttgggtaaa cataatcccc
     1021 tatattttac ggaacttcta tttcgtccag aatgcgattg atgatatctt cgctggtaag
     1081 ttcttcggct tcagcttcgt aggaaagaat aatacgatgc cttaaaacat cttttgctat
     1141 catttttatg tcatcgggaa ttacataagc cctacctgaa atgtaagcat tcgttttagc
     1201 agctcttgcc aggaaaattg ttgctcgggg agaagcacca aattcaatca gacccttcaa
     1261 atcgtttaaa cgcggatagc gttccggatg gcgggttgaa ttgaccagat gtaaaatata
     1321 atccttcagc ttttcttcca tatacacctt atgcatcact tccctcattt cttcaatatc
     1381 tttgggactt agcactttct gcaagggaat gggttgttca ttaaccattc tttccaggat
     1441 ttgcctttct tcatcatagg caggatattt aatcttcagc ttcataaaaa accggtctat
     1501 ctgcgcttct ggaagcggat aagttccttc ctgctcaata ggattttgag ttgccatcac
     1561 aaaaaacgga ttgggcaaag gaaaagtgtt atcccctaaa gtaacctgcc gttcctgcat
     1621 tgcttctaaa agagctgatt gcactttgga aggagctcta ttaatttcat ccgctaagat
     1681 gaaattggca aagacaggac cttttttcac gctaaaatcc ccgcttttag gattgtaaat
     1741 cattgtgcct gttatatctg caggcaacaa atccggagta aactgaatgc gggaatagga
     1801 agcatcaaaa acggatgcca gcgatgaaat aatcagggtc tttgccaaac ctggaacacc
     1861 ttcaatcaaa atgtgaccat ttgctataat tccaatcagc aagcggtcta taatttcctg
     1921 ttgaccaact atcactctgg caatttcaga acgcacatta cccaatacag cagaactttt
     1981 ttctacctcg ttttggattg cctcaattaa cattaaaccc ccataatagt gtattagtta
     2041 gatagttatt tagtgctttg gttattcatt attttaacac aaaaatcggg aatccggcaa
     2101 aaccgcgatt cccgataaac atatttctct gcttgattat aagccaaaaa accgattgtt
     2161 attatacggc tttgcggact ttatttgctt tcaggcaaga actgcaaacc ttaattttct
     2221 tagttccgcc atcaccggca acacgaattt cgtgcaaatt aggataaaac ctacgcttgg
     2281 tagcatttaa agcatgactg cgatgattac ctacctgagc tgtttttcca catatatcac
     2341 atactttgga cataattcac ttcccgtttt ttcttatgat aaagaccaaa aaaaagaggg
     2401 ctctgttttt gacaagcaat tatttagtgt tctgcggaat acgaatatca aatctactgc
     2461 catgaggttc tacgggaata tattccaaac gcccgtgatg ctcctgaaca agttttttac
     2521 atatagccaa acctaaaccc gttccctgtg ttttaccatc cgtaacgaag ggctgaaata
     2581 attcttcctt cactttttgg gaaacacctg gtccgttatc aattaccgaa atctgtaacc
     2641 acccggaaga aacactgctg ataatcttga tttcacctgc ttcagaaatg gcttccaagg
     2701 catttttaat gaggtttatt aagaccctgc gaattttacc ttcatcaaaa tagacacaat
     2761 cattaacctt gttttccact acaaaattga tcttgcgttc tttaagagaa gcggaataaa
     2821 gctcacttat ctcctgtaaa aagctatcca aattaacttt ctgaatcagg ggaggacttt
     2881 ctgttcctct ggcaaaatcc aaaatttcat gcaccagttg gtctatcagt tttgtttgtt
     2941 taacaatgtt atccgtaaat tcagcacttt cgggaaaaat attctccagc aactgtgccg
     3001 ttagcacaat aactgttaag ggggttttaa tatcgtgaat aattttgctg gctgccaatc
     3061 caatagccat taaacggttc tttttcagca tttcatccat cagttctaca aatctttcat
     3121 tagtattacg caagcgactg gaaattacct tagttaaatt caacattat
//
LOCUS       DAOPFL010000139         3166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_42469_length_3166_cov_74.173899, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000139 DAOPFL010000000
VERSION     DAOPFL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42469_length_3166_cov_74.173899"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2973
                     /locus_tag="PLG69_07175"
     CDS             <1..2973
                     /locus_tag="PLG69_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43325.1"
                     /translation="NERIPIYSSQFLEYYGNGLTLPNGDILFFTNASDEYGNNIVMNR
                     ITVIGENVSSESIDITNAPGDEYIIDQVISSDGCVIFTYNFLAEDVDHYYDDVMYIQK
                     VTLSGQTLWGEMGKKITRNFTNYALVPNNLGGAYLLNDGNSYDEAKVYGWNFNTQGNN
                     LWQSDIILEMPCSYLYLNNVLDDGAGNIILNLMLKDSPESDWYSHLIKLSPEGNIIGN
                     NPLLPQSQFSGLYQIHSDRNGNYVLWKVIDSNLTLQKMDSSYNLLLPQAITYQHSSDS
                     EISNLGFCADGSLYYSEERNYPLNPLIYKLNPDLQPSWTAPAEIPFYYNYYYRTFRIA
                     DDNSLYMAYIDYWGSNGNLPNSSIMLTRINSVDGSIAFPFTCVSSQIFPKGAARIFAS
                     SDKALVLWYDISDINFEVRCQLVAPSGSILLEQEGRTLQYRQTGSVNNYQVVNLPQGA
                     CYVYEITDTCFNFKLYYQICDDNLSPLLGEYGIELNPDSSEAEWLKKIILTPQNQIGI
                     LYLTQNAQDVFSLYYQVIDNTGTVLLPGRGILIKEAVTDPNYNYQMTCVDDDIIIAWE
                     EIYSLLITRIIRGQRLHNNLPQWDAGGKILKQSTPYNCNLVNICENYLVYEDYNYTNY
                     SYGIRKLDTDGNPDPSWLLTDYITSGIHSFLMQSCSLMGDEMAVFYIKEFIAGLYARY
                     DLYVQKYNSQGLPLWGTLGILLDTTEGRPISVAGVNYGDKIFFALQKYDPYLYINYMS
                     YNLITNNGTIIWDNPESFTTEMFYETNDFKLIPYNNDVYSILWLSVRSDNLSKINNHY
                     VLSDGSLYYNQAAIIDKSVKSISDLQVLNKGNYCLANYRLNKYYIEYKGAAEGLQSSA
                     SNESRGLRSTASNPKGFSYEVKSLYAYKIPSEPVFTDDPLQPPATLSCCNYPNPFNPE
                     TTISFEIPKTDNVSLQIYNLKGQLIRTLVNDYLPAGKQSIVWNGTDNNNQPVASGVYL
                     YKIKTGKCTKSAKMLLLK"
BASE COUNT          983 a          588 c          553 g         1042 t
ORIGIN      
        1 aatgaacgaa ttcccatata tagttcacaa ttcctggaat attacggtaa tggtcttact
       61 ttgcctaacg gtgatattct cttttttacc aatgcctctg atgaatatgg taacaatatt
      121 gtgatgaaca gaataaccgt catcggcgaa aatgtgtcct ctgaatcaat agacattacc
      181 aacgctcctg gtgatgaata tatcattgac caagttataa gttctgatgg atgcgttatt
      241 tttacctata atttcctggc agaggatgtt gatcattatt atgatgatgt aatgtatatt
      301 caaaaggtta ctcttagcgg tcaaacctta tggggtgaaa tgggaaaaaa gataacccgt
      361 aattttacca attatgcctt agttcctaat aatttaggtg gtgcctatct gcttaatgat
      421 ggtaattctt atgatgaagc caaagtttat ggctggaatt ttaacactca gggtaataac
      481 ctctggcaat cggatataat tttggaaatg ccttgctctt atttatactt aaataatgtt
      541 cttgatgatg gagcagggaa tatcatctta aacttaatgt taaaagattc tccggaaagc
      601 gattggtaca gccatttaat caaactttct cccgagggta atattattgg taataatcct
      661 ttactgcccc aaagccaatt ttccgggtta taccaaattc acagtgatag aaacgggaat
      721 tatgttttat ggaaagttat tgattccaac cttactttgc aaaaaatgga ttcctcgtat
      781 aatttgcttt tgccgcaagc aattacttat cagcatagtt ccgatagcga aatttccaat
      841 ctcggatttt gtgcagacgg cagcttatac tattcggaag aacgcaatta tcctcttaat
      901 cctttgattt ataaattaaa tcctgattta caaccaagtt ggactgcccc tgcggaaatt
      961 cctttctact ataattatta ttatcggact tttcgtatag ccgatgataa ttctttatat
     1021 atggcatata ttgattattg gggttctaat ggtaatttac ctaattcatc tataatgctt
     1081 acccgcatta attctgtaga tggcagtatt gcttttccgt ttacttgtgt ttccagtcag
     1141 atctttccca aaggtgcagc aagaattttt gcctcttctg ataaagccct tgttttatgg
     1201 tatgatatat ccgacatcaa ttttgaagtt cgctgtcaac tggttgcacc ttcaggttct
     1261 attttattgg aacaggaagg cagaactctt caatatagac aaacaggttc tgtgaataat
     1321 tatcaggtcg ttaatttgcc tcagggtgcc tgctatgttt atgaaataac tgatacctgt
     1381 ttcaacttca aactctatta ccaaatctgc gatgataacc tttctccctt actgggggaa
     1441 tatggaatag agctaaaccc cgattccagc gaagcagaat ggttaaaaaa aattatactg
     1501 acaccccaaa accagattgg tatcctctat ttaactcaaa atgctcagga tgtatttagt
     1561 ttatattatc aagtgataga taatacaggt acagttttat tgcccggtag gggtatcctg
     1621 attaaagagg cggttacaga tcctaattat aactatcaaa tgacttgcgt ggatgacgat
     1681 ataatcattg cctgggagga aatatattct ctgctgatca caaggattat aagaggccaa
     1741 cggttacata ataaccttcc ccaatgggat gcaggaggaa aaattctcaa gcaaagtact
     1801 ccgtataatt gcaacttggt gaatatttgt gaaaattatc tcgtttatga ggattataat
     1861 tacactaact attcctatgg aataaggaaa ttggataccg atggaaatcc tgacccttcc
     1921 tggctgctta ctgactatat cacaagtggc atccattctt ttttaatgca atcttgctca
     1981 ctaatgggtg atgaaatggc tgtcttctat attaaagaat tcattgcggg tttgtatgct
     2041 aggtatgatt tgtatgttca gaaatataat agccagggat tacctttatg gggaactttg
     2101 ggaattctct tagatactac cgaaggtaga cctataagcg tagcaggtgt taattatgga
     2161 gataaaatat tttttgctct ccaaaaatac gatccgtatt tatatataaa ctacatgtct
     2221 tataatttga taactaataa tgggactata atttgggata acccggaatc ctttaccact
     2281 gaaatgtttt acgagaccaa tgattttaag ttgatacctt ataacaacga tgtttactcc
     2341 attctttggc tgtctgtgcg tagcgataat ttaagtaaaa ttaataatca ttatgtcctc
     2401 tctgatggct ctctttacta taatcaagct gcgataattg ataaatcagt taaaagtatt
     2461 tctgatttgc aagtgcttaa taaaggaaac tactgcctgg caaattaccg cctcaataaa
     2521 tactatattg aatacaaagg tgcagctgaa ggactccaat cctccgcatc caatgaatcc
     2581 agaggactcc gatccactgc atccaacccc aaaggatttt cctatgaagt aaaatccctc
     2641 tatgcttaca aaatcccttc cgaacctgtt tttacggatg accctttgca acctcctgcc
     2701 accctttctt gctgtaacta tcccaatcct ttcaatccgg aaaccacaat cagctttgaa
     2761 attcctaaaa cggacaatgt ctctctccaa atctataacc tcaaaggaca gctaatccga
     2821 actcttgtta acgattatct tcctgccgga aaacaatcta ttgtttggaa tggaacagat
     2881 aataataatc aacctgtcgc cagcggtgtc tatctttata aaataaaaac aggtaaatgc
     2941 actaaatccg ctaaaatgct cctgctgaaa taaactttta aagttaaact gctattctat
     3001 ttcagtatac cattttaggg cgaaaatttc tttgtcattc ctatgcaagc tggaatccat
     3061 acattatcca ataaaaaata atttgctttt ctaatgaact gcattataat gtaattaggt
     3121 taaggtggag agggattttt caggtagttg aaaaaaagcg gttgac
//
LOCUS       DAOPFL010000140         3114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_43403_length_3114_cov_114.788820, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000140 DAOPFL010000000
VERSION     DAOPFL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43403_length_3114_cov_114.788820"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2126
                     /gene="alaS"
                     /locus_tag="PLG69_07180"
     CDS             <1..2126
                     /gene="alaS"
                     /locus_tag="PLG69_07180"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPI43326.1"
                     /translation="FWEMGETGPCGPCSEIHIDRGISHCTRQNEPGHICKINGGCSRF
                     IELWNLVFIQYNREADKTLSPLKNKFVDTGAGFERLAQVLQDKNSNYETDLFMPLINK
                     IEELSGVDYTQETGMPHRVIADHIRCLCFALADGGFPSNEGRGYVLRRILRRAARYGR
                     LLGFAEPFLHLLVPLVIEQMAHHFSELNGKEDYIKMVIKAEEERFNKTLDTGLEKFRE
                     ITQKLKGEVISGSDAFILYDTYGFPPDLTAILAEEKGLKIDYEGFEKEMQLQRERARK
                     ASKFTLFVNNEDWIEFSPVTPTEFVGYKENTVQTYIQRYAIQEKGLILMQLAQTPFYA
                     ESGGQVSDTGKIYNEEFELEITNVRKQDEYIIHYGKLKRGTVNNAPVTAEIDRERRKS
                     IARNHTATHLLHKALREVLGEHTVQKGSLVHPDYLRFDFAHFRALTLEEIRKVEDIVN
                     QAVLDNRKVNTTVKNIEEAKKEGAIALFGEKYSEKVRVVCVENFSQELCGGTHISSTG
                     EIGLFKIISESSSAAGIRRIEAITGISALKWVQDLQDKLSRISTLLNSPVKNIESKLE
                     ATLEQISALERELKSAKAKEEEKFVQELLSKATKQENYLLIKAQTEFDNLEELKTTAE
                     QLKTKMQGTIAVLFNLYQDKLNILCVVSADLIPKYNAGKIVAKLANELKGKGGGRTDI
                     AMAGGKDIANLASVMEKVPEIIASI"
     gene            2127..3104
                     /locus_tag="PLG69_07185"
     CDS             2127..3104
                     /locus_tag="PLG69_07185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPI43327.1"
                     /translation="MNILILRLSSMGDIILTQPICAILQKHFAGCQIDYVCKEEFKEL
                     PEMFAPPVHPLVYEKTLKFHLWLSKRKYDLILDLHNKFSTFLLMLFLRAPRKVHYSKK
                     RGLRRDIVKGNKKLKIDSTVELYASALKKLGINEPWSYPRLQVTELPNSAPAKHNNAL
                     SGEIKIAVFPGANHFTKRYPAANWIQLINSNPQYDFTLFGSQADEELCKFITQKCSTN
                     CRNKCSTLTLQELLTELISYNLIISGDTGPMHLAATLNKPQIAIFGGTHPRLGFRPLN
                     DKAIILCAELPCQPCSLHGKEKCPLNHFNCMNAISPALLSSAIKKALEG"
BASE COUNT         1003 a          580 c          649 g          882 t
ORIGIN      
        1 atttttggga aatgggggaa acaggaccct gcggtccctg ttcggaaatt catatagata
       61 gaggtatttc gcattgcacg aggcaaaatg agccaggaca tatctgtaaa ataaatggcg
      121 gttgttcgcg ctttatagaa ttgtggaatt tggtttttat tcaatataac cgcgaggcag
      181 ataaaacact ttctccgctc aaaaacaaat ttgtggatac aggagcgggt tttgaacgcc
      241 tggcacaggt tttacaggat aaaaacagta actatgaaac agaccttttt atgcctctga
      301 taaataaaat tgaggaactt tctggggttg actatactca ggaaacagga atgccgcata
      361 gagttattgc cgatcacatc cgctgtctct gttttgcttt ggcagatggt ggttttcctt
      421 ccaacgaagg aaggggttat gttttacgca gaattttaag aagagctgcc cggtatggtc
      481 gtttgctcgg ttttgctgaa ccctttttgc atttgttagt gccgcttgta attgagcaaa
      541 tggcacatca tttcagtgaa ctaaacggta aagaggacta tataaaaatg gtgattaaag
      601 cggaagaaga acgctttaat aagaccctgg ataccggtct ggaaaaattt agggagataa
      661 cccaaaagct aaaaggagaa gttatttctg gttccgatgc ttttatttta tatgatactt
      721 acggttttcc gcccgattta accgctattt tagcagaaga aaagggcttg aagattgact
      781 atgagggctt tgaaaaggag atgcaattac aaagagaacg cgccagaaag gcaagtaaat
      841 ttactctttt tgttaataat gaggattgga ttgagttctc tcctgtaact cctactgaat
      901 ttgtcggtta caaagaaaat acggtgcaga catatatcca acgctatgcc attcaagaaa
      961 agggtttaat ccttatgcaa cttgcccaaa cacctttcta tgccgaatcc ggtggacagg
     1021 tatcggacac aggaaaaata tataatgagg aatttgaact ggaaataacc aatgtccgta
     1081 aacaagatga gtatatcatt cattacggaa aactgaaaag aggaactgta aataatgcac
     1141 ccgttacagc agaaatagat agggaaagac gcaaaagcat agcccgaaat catacggcta
     1201 ctcacctttt gcataaggca ctcagagagg ttttaggaga acataccgtt caaaagggct
     1261 ctttagtgca tccggattat ctgcgttttg attttgctca tttcagagct ttaaccttgg
     1321 aagaaatcag aaaggtggaa gatattgtta atcaagcagt tttggataac aggaaagtga
     1381 atactacggt taaaaatata gaagaagcca aaaaagaagg tgccattgct cttttcggag
     1441 aaaaatacag tgaaaaagtt cgtgttgtat gcgttgagaa tttttcccaa gaactttgtg
     1501 gcggaactca tatatcttct acaggtgaaa tcggcttatt taaaattatt tccgaaagct
     1561 cttccgcagc aggaattaga agaattgagg caataacagg tatttctgcc ttaaaatggg
     1621 ttcaggattt gcaggataaa ctaagccgta tttctacact gctgaattct cctgttaaaa
     1681 acattgaaag caaattggaa gcaactttgg aacaaatttc cgctttggaa agagaactaa
     1741 aatccgctaa agccaaagaa gaggaaaaat ttgtgcaaga actgctctct aaggcaacga
     1801 aacaggaaaa ttatttgttg attaaggcac aaactgaatt tgacaatttg gaagaactta
     1861 aaacaaccgc tgaacagcta aaaaccaaaa tgcagggtac gattgccgtc ctgttcaatc
     1921 tctatcagga caaactgaat atcctgtgtg tggtaagtgc agaccttatt cccaagtata
     1981 atgccggaaa gattgtggct aaactcgcga atgaattaaa aggaaaaggt ggtggcagaa
     2041 ctgatattgc tatggctggc ggaaaagata tagctaacct tgcttctgta atggaaaagg
     2101 tgccggaaat aattgcttct atctaaatga atattcttat tctgcgactc tcttcaatgg
     2161 gtgatattat actcactcaa ccgatttgtg ctatcttaca aaaacacttt gccggttgcc
     2221 aaattgacta tgtttgcaaa gaggaattca aggaacttcc ggaaatgttt gctcctcctg
     2281 ttcatcctct tgtttatgaa aagacgctga aatttcattt atggctaagc aaaagaaagt
     2341 atgacctaat tctggatttg cacaataagt tctctacttt tttgctgatg ctttttttaa
     2401 gagctccccg aaaagtccat tacagcaaaa aacgcgggct tagacgcgac attgtgaaag
     2461 ggaataaaaa gctgaaaata gacagtacgg ttgagcttta tgcctctgct cttaaaaaac
     2521 tgggaataaa tgaaccctgg tcttatcctc gtttgcaggt aactgagcta ccgaattccg
     2581 ctcctgcaaa acacaacaat gccctctccg gcgaaataaa aattgccgtt tttccgggtg
     2641 ccaatcattt taccaaaagg tatcctgcgg caaactggat tcaactgata aactccaatc
     2701 ctcaatacga ttttactctt tttggttccc aggcagatga agagctatgt aaattcataa
     2761 cccaaaaatg ctccactaat tgcagaaata aatgctctac gcttactctg caggaacttt
     2821 taacggaact gatttcttac aacttaatta ttagcggtga taccggacct atgcacttag
     2881 ccgcaacttt gaataaaccc caaatagcca tttttggagg aacacatccg cgcttgggct
     2941 tccgaccttt gaacgataaa gctattattt tatgcgctga actgccttgc caaccttgct
     3001 ctttgcacgg aaaagaaaaa tgccctctta atcatttcaa ttgtatgaat gctattagtc
     3061 ccgctttgct tagttctgct attaaaaaag ccctggaagg ttgatattct ctaa
//
LOCUS       DAOPFL010000141         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_44395_length_3064_cov_86.378863, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000141 DAOPFL010000000
VERSION     DAOPFL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44395_length_3064_cov_86.378863"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1572
                     /locus_tag="PLG69_07190"
     CDS             328..1572
                     /locus_tag="PLG69_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPI43328.1"
                     /translation="MAEKLKLARSMSLFEEAKKLVPGGVAGIRRPYNFVPGEYPIFFD
                     HGRGGRVVDVDGNEYIDFLCAYGPIIIGYREDEIDDAVINQIKNKGFCFSLTQEMQNT
                     LVKKMRELIPCCEMAALVKTGSDATTIAIRVARGYTGKTKIARYGYHGWHDWCVEVKG
                     GIPPKLYEDIYEFHYNDLDSLKAILEANKDDMAGIIITPIGHPNGAEVQMPKPGYLEA
                     VRELANQYHCLLIFDEIRSGFRCSLGGAQKLFGVTPDLSTFGKAMANGYAIAALVGKE
                     EYMQVLADKVFLSSTFFPNSDGIVAAIKTIEILERDRILDVVAAKGRKFGAEVEKVVE
                     ESGVPVNFTGAPWMPYITFKKDEAGLYKKLRTEYYTQLIRRNVFMQPYHHGYICYRHT
                     DEDLAYTVEAIRESLAEVKKML"
     gene            1585..>3064
                     /locus_tag="PLG69_07195"
     CDS             1585..>3064
                     /locus_tag="PLG69_07195"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPI43329.1"
                     /translation="MAKYIFVMGGVLSSLGKGIATASIGFLLKSMGYKVVLQKFDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFVDEALTKYSSNSAGQIYESVIENE
                     RKGVYLGKTVQVIPHITNEIKGRIQRLANDYDIVITEIGGTVGDIESLPFLEAVRQLR
                     LDLGYYNTLYILLTYVPYIKSAGELKTKPSQHSAYKLREIGIQPDILLCRSEKPFAED
                     IYNKIALFTNVPRENVINAIDVDCVYEIPLNFQKAGLPEIICQHFKLEQRPIIMQDWL
                     QFLENTKNAKETVTIAVCGKYIQHQDAYKSIREALLHAGSALLKKVEIIWIDSERNFK
                     EEDIAKKLEGVQGILVPGGFGVRGIEGKIAVAKYARENNIPYLGICFGMHIAVIECAR
                     NLANLPGASSIEFDEFTPNPVIHLREDQLHRDKVGGTMHLGAFQFNLVKGSKAFEIYQ
                     TETISERHRHRYEFNNDYREVIEKAGLKISGLSPDGLLVEIVE"
BASE COUNT         1004 a          461 c          688 g          911 t
ORIGIN      
        1 aaagagaata attgagaatg aaaaatgtaa aatgtagaat gtagaatgat aagaggcaga
       61 ttacgcagat tttttaaaga gataagagaa ttagtgattt aatatagaat gcataaataa
      121 gatagaattg gtgtggaata aaaggaaaaa atagaaggtg tcttgttatt ttatcaagac
      181 ccattctata ttataaatct cttaaatccc tttaatcctg tttatcccag acctattatt
      241 tttttaatct gcggaatctg cgaaatatgc gtgccaaact ttgaactttg aactttgaac
      301 cttgaactta actaaggagt atataaaatg gcagaaaaat tgaaattagc cagaagtatg
      361 tctctttttg aagaggcaaa gaaacttgtt cccggaggcg ttgctgggat aagaaggccc
      421 tacaattttg ttccgggtga atatcccatt ttctttgatc acggaagggg cggcagagtt
      481 gttgatgtgg acggtaacga atacattgac tttctttgtg cctacggacc tattattatt
      541 ggttaccgtg aagatgaaat tgacgatgcc gttatcaatc agattaagaa caaaggtttt
      601 tgtttttccc taacgcagga aatgcaaaac actttagtga aaaaaatgag agaactgata
      661 ccctgttgtg aaatggctgc tttagtaaaa acaggtagtg atgctacaac tattgctatt
      721 cgtgtagctc gcggttatac aggcaaaact aaaatagcgc gttacggtta ccatggctgg
      781 cacgattggt gtgttgaagt gaaaggtgga attcctccca agttatatga agatatttat
      841 gaatttcatt acaacgattt ggattcctta aaagcaattc tggaagccaa taaagatgat
      901 atggcaggaa taattattac tcctattggt catcctaatg gagcggaagt ccaaatgcca
      961 aaaccgggtt atctggaagc agtgcgtgaa ttggcaaatc aatatcactg tttgttaatt
     1021 tttgatgaaa tccgcagtgg ttttagatgc agtttaggag gagcgcaaaa attatttggg
     1081 gtaacacctg atttatctac tttcggtaag gcaatggcaa atggttatgc tattgcagct
     1141 ttagtaggaa aagaggaata tatgcaggtc ttggcagata aggtatttct atcctcaacc
     1201 tttttcccca atagcgatgg catcgtagct gctataaaaa ccatagaaat attggaaaga
     1261 gaccgtattt tggatgtggt tgccgcaaaa ggaagaaagt ttggagcgga agtagaaaaa
     1321 gtagtggagg aatcaggagt gcctgttaat tttaccggag ctccctggat gccttatata
     1381 acctttaaaa aggatgaggc gggactttat aaaaagttac ggacagaata ttatacccag
     1441 ttaattaggc gcaatgtttt tatgcagccc tatcatcatg gctatatttg ttatcggcat
     1501 acggatgaag atttggcata cacggtggaa gcaattagag aatcccttgc cgaagtgaaa
     1561 aagatgttat aagagataag ctaaatggca aaatatatat ttgtaatggg tggcgtgctt
     1621 tcttctttag ggaagggcat tgccaccgct tctattggct ttttgttaaa atcaatgggc
     1681 tataaagttg tgctccaaaa gtttgatcct tacttaaatg ttgacccggg aacaatgagt
     1741 ccttttcagc atggtgaggt ttttgtaacg gatgatggag cagaaactga tttggatttg
     1801 gggcattatg agcgttttgt ggatgaagcg ttaactaagt attccagcaa ttctgcaggt
     1861 cagatatatg aaagtgtgat agaaaatgaa cgcaaaggtg tatatttagg caagacagtg
     1921 caagttattc cgcatatcac aaacgaaata aagggtagaa ttcagcgttt agcaaatgat
     1981 tatgacatcg tgattacgga aattggaggg acggttggtg atattgaaag tttacctttt
     2041 ctggaagcag tgcgtcaatt gcgtttagac ctgggctatt acaacacgct gtatattctt
     2101 ttaacttatg ttccatacat caaatcggca ggagaattga aaactaagcc ctcacaacat
     2161 agtgcttata aattacggga aataggaatt caaccggata ttttgctttg ccgaagtgaa
     2221 aaaccttttg ctgaagatat ttataacaag atagctcttt ttaccaatgt gccaagagaa
     2281 aatgtaatca atgccattga tgtggattgt gtttatgaaa ttccactcaa ttttcaaaaa
     2341 gcaggtttac cggaaattat ctgtcagcat tttaagttag aacagcgacc aatcattatg
     2401 caggactggc tccagttttt agaaaacacc aaaaatgcca aagaaaccgt tacaattgct
     2461 gtttgcggaa aatatattca acatcaggat gcctataaaa gcattagaga agcactttta
     2521 cacgccggtt ccgctttact aaaaaaggtg gaaattatct ggatagatag cgaacgcaat
     2581 tttaaggaag aagatatcgc taagaaactg gaaggagttc aaggaattct tgttccaggt
     2641 ggttttggcg taagaggcat tgaaggcaaa attgctgttg ctaaatatgc ccgggaaaat
     2701 aatattcctt atttgggtat ctgtttcgga atgcatatag ccgtaattga atgtgccaga
     2761 aatttagcta atttaccggg tgctagtagc attgaatttg atgaatttac acccaatcct
     2821 gtgatccatt taagggaaga tcaattacat cgggataaag ttggcgggac aatgcattta
     2881 ggtgcttttc aatttaattt ggtaaagggc agtaaagcat ttgagattta tcaaacagaa
     2941 actatcagcg aaaggcacag acatcgctat gaattcaata atgactatcg tgaagtaata
     3001 gaaaaagcgg gacttaaaat aagtggttta tctccggatg gacttttggt agaaattgta
     3061 gaat
//
LOCUS       DAOPFL010000142         3023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_45234_length_3023_cov_102.735175, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000142 DAOPFL010000000
VERSION     DAOPFL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45234_length_3023_cov_102.735175"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      134..252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            295..1107
                     /locus_tag="PLG69_07200"
     CDS             295..1107
                     /locus_tag="PLG69_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43330.1"
                     /translation="MKKFSLLLLVFLTLLMFSCSEKDDNPTNVDFYGYKLDQFISNTI
                     LRDLIDPNASDTLNFRELFAYEIEASDGFSPRNSSYAGYDLTWNFFKEGYIVPADNNR
                     TWFPDELGLPNAFKVKSTTQFNLYRKVEVTSGESSKTIELKGLTIYTIPNWNSANEEA
                     IKLSDLLQGISLSDSSNIRLIAIDDYYKDYTPEQIAEGYYLLDSEVTTFPEINDTLPG
                     ALKKFKKLASIEVTNGSSQDHNFANAPHDKKDIAIPVPANFSGYEATPLPDY"
     gene            1129..3015
                     /locus_tag="PLG69_07205"
     CDS             1129..3015
                     /locus_tag="PLG69_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPI43331.1"
                     /translation="MKQLKIDLLLILVLVTITLCGTAKQDSLKTYTLPTVYVIVEKPS
                     EAIGSLHIIEGEDVKNALSLREAMQNSVGISATIGTKDESNLRLRGFRKNEIKVMVDG
                     KPLNNGYFGNVDISKLSLLDIEEIQILKGPASPLYGTNNMGGVVNIITKAPPNNYWLE
                     LNTLFKRNNTNEITLSSAHSFDTWNYRIGVSQEKTDGFVLSEKFIPTPYEDGGVRNNS
                     DKCLYNISGSLEKEFLNFHNLGVDFGYNKMDKKNIPSSIYEQRYRQYQDWMRYHIGLW
                     TKMQVSESGTLNLQLINDGSTDRYLEYKDPAYENLALDSKMHNAGFGFHSRFTKTFTG
                     NKKLDIGYNYDFQGNNRKDNDSYQQWTDSVVHIHQAFTQFECTIIPKLLLTSGIGVNT
                     RINRDNNKIQPQLEPAVGIVYEIIPDSETSFAIGRNTSHPTMRQLYSASKGNPDLKPQ
                     NSLKLEINHNQALWQNKISLSFSVYFNDTKDLIDLYNGRYENIYKVQSQGTEIGVSFS
                     PDKFFQTSVNYAYLSYRKISDYYLTETPKNSLELCQQFTFPYKIGFRINSSYRDRCLS
                     QDDDGRYHTLAPYWKHDLILTIPWKRLKLSIAVENVLDEDYQSEYGYPEPGRNFSLGL
                     QAKI"
BASE COUNT          994 a          573 c          547 g          909 t
ORIGIN      
        1 cggaactgat actagaaatc ttgtaaatcc cttttatcct gttcatccca tacctattct
       61 catttttaat ctgcgtcatc tgcgaaatct gcgtgaacat taagttgggg ttgacaaatt
      121 ttcctgcttt ggattttttg aaaaaagccg agcgcatatt cctaaacccc tttggtatgg
      181 aattgcgcca ttgggtataa ttggaaacga ttatgcctcc cgtatttgga aaggcaacag
      241 tggaaatata acttttcatc cctgtctgcc tctatatctt attatggagg aaaaatgaaa
      301 aaatttagct tacttttact cgttttcctt acgctgctta tgttttcctg cagtgaaaag
      361 gatgataacc caacaaatgt tgatttctac ggttacaaac tggatcaatt tatttccaac
      421 actattttac gcgatttaat tgatcccaat gcaagtgata ccttgaattt ccgggaatta
      481 ttcgcttatg aaattgaagc gagcgatgga ttcagtcccc gtaattcatc ttacgccggt
      541 tacgatttaa cctggaactt ctttaaagag ggctatattg tccctgccga caataacagg
      601 acctggtttc ccgatgaact tggacttcct aatgctttta aagtaaaaag cacaacccaa
      661 ttcaatttat accgcaaagt tgaggttacc agtggagaaa gcagtaagac cattgaattg
      721 aaaggtttaa caatttatac tatcccgaat tggaattccg caaatgaaga agcaattaaa
      781 ctcagcgatc ttttacaggg tatcagttta tccgatagca gtaatattcg tttaatcgct
      841 atagatgact attataaaga ttataccccg gaacaaattg ctgagggtta ttatctgctg
      901 gattctgaag ttacaacttt tcctgagata aatgatacat tgccaggagc gctaaaaaag
      961 tttaagaaat tggcaagcat agaagtgaca aatggcagct cacaagatca taattttgct
     1021 aatgcacctc acgataaaaa agatatagct attccagttc ctgccaattt cagcggttat
     1081 gaagctactc cattaccgga ctattaaaaa aactatttcg ccaaagaatt gaagcagctg
     1141 aaaattgacc tgctactaat cttagtatta gttaccatta ctctatgcgg aacagcaaaa
     1201 caggattcgc tgaagaccta tactcttccc accgtttatg taatagtaga aaagccctca
     1261 gaggcaattg gttctctgca tataattgaa ggtgaagatg taaaaaatgc gctttcttta
     1321 cgcgaagcaa tgcaaaatag cgttggcatc agcgctacta ttggcacaaa agacgaaagt
     1381 aatttacgct tacgtggttt ccgcaaaaac gaaatcaagg tgatggtaga tggcaaaccc
     1441 ttaaataacg gttatttcgg caatgtggat atcagtaaac tctctttgct ggatattgaa
     1501 gaaattcaga ttttgaaggg tcctgcttca cctctttacg gaacaaataa tatgggtgga
     1561 gttgtaaata ttattaccaa agccccgcca aataattact ggctggaatt gaatacactc
     1621 tttaaacgca acaataccaa tgaaataacc ctatcttccg ctcatagttt tgatacctgg
     1681 aattaccgta taggagtttc ccaggaaaaa acagacggtt ttgtgctctc cgaaaaattt
     1741 attcctaccc cttatgaaga tggaggagtt cgtaataaca gcgataaatg cctctataac
     1801 atttccggtt ctctggaaaa ggaattctta aatttccata atttaggtgt ggactttggc
     1861 tataataaaa tggataaaaa gaatattcct tcttctattt atgaacaacg ctataggcaa
     1921 tatcaggact ggatgcgtta tcatatcggc ttatggacta aaatgcaagt gagtgaatcc
     1981 ggcactttaa atctgcagtt aatcaatgat ggaagcaccg atcgctattt ggaatataaa
     2041 gaccctgctt atgaaaattt agctttggat tctaaaatgc acaatgccgg ttttggcttt
     2101 cattcccgtt ttactaaaac atttacggga aataaaaaac tggatattgg ttataattac
     2161 gatttccagg gcaataaccg gaaagataat gattcttacc aacaatggac ggattcggtt
     2221 gtccatattc atcaggcatt tactcaattt gaatgcacca ttattcctaa gttgttatta
     2281 accagcggaa taggagttaa taccagaata aatagagata ataacaaaat tcaacctcaa
     2341 ttggaacccg ccgtaggaat tgtgtatgaa ataattcctg atagtgaaac atcttttgct
     2401 ataggtcgca atacttctca tccaactatg cgtcagcttt attcagccag taagggaaat
     2461 cccgatttaa aaccgcaaaa ttctctgaaa ctggaaatta atcataatca ggcactttgg
     2521 caaaataaaa tttctctgtc tttcagcgtt tattttaacg ataccaaaga cctgattgat
     2581 ttgtataacg gacgctatga aaatatctat aaagttcaat cacaaggaac agaaatcgga
     2641 gtaagctttt ctccggataa atttttccag accagcgtta attatgctta tctcagctac
     2701 cggaaaataa gcgattatta cttaactgaa acaccaaaaa attcccttga attatgccag
     2761 caattcactt ttccttataa aatcggattt agaataaaca gttcctacag agacagatgt
     2821 ctttctcaag acgatgacgg aagatatcat actttagctc cctattggaa acacgattta
     2881 atattaacca taccctggaa acgacttaaa ctttctatag ctgtggaaaa tgtcttggat
     2941 gaagattacc aaagcgaata tggatatcca gaaccgggaa gaaacttttc tcttggtttg
     3001 caagcgaaaa tataggtcgg gag
//
LOCUS       DAOPFL010000143         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_45634_length_3004_cov_122.851475, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000143 DAOPFL010000000
VERSION     DAOPFL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45634_length_3004_cov_122.851475"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2968
                     /locus_tag="PLG69_07210"
     CDS             <1..2968
                     /locus_tag="PLG69_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI43332.1"
                     /translation="IESPLKAKIGLTYSNLYVGVLGAKQRCEFGIIGTGVNVSARLMT
                     SAKYGQIVFTKDILPSVQSRFEVQFLRKERVKGIKDELFFYCILRELPEFLNSYKRQY
                     QNKTQICYQEQTAEIIQKIKDKKINQVLISGDHGTGKSFLSWQILNNFYEENYKISIF
                     VLDEFNHHDPLILHRKFISNELELNDPLSEPEKLQKYLGEILEERDADILLNALGLQN
                     KETILTNDSGKQIEFLLLSLQKSLDLLMRDFDLILLDNIQWLDDLSAQILQKRLEDDK
                     PKSQTLILTTTKEITNYQNKANAKTEFISLRELNREEVIALIRSQIPNITFQAVDYIY
                     NLAGGNPRFITELCSQILSSFPDPDMLITESNIYDIQNKGLLPYSVENLFIMKYESLS
                     QEAKDILKKASIIGKGFTLNEIFETQSGISQNEIIPVISELQNNEIIDITTLSPEVQY
                     LFNNALMRQAIYSTILLGEKVSLHNRIASFYEEKYGTLANRHSELLAYHFHLGENKEK
                     ALHYALIAGDQNQKINNHSEAIYYYQIALQHTTERLEKIAIILSIVDSQLYLSEVEVA
                     KENLETIEPKEISLPELLKKYHFLRCRVYYLSGDYEGVLKYLKNVTDFEGKYGEQIRV
                     YQLDCLYRLFLIEDFSALLKDLKQEFVQQAANTLNVKSQKPSISTLLSRFQKIPEDEI
                     TEEQKHYLYLLLKLESIEANHFINTGYYQKALKSLLSQYELAKALKDDLSLRIASNRL
                     GVVYTRMGNLDSAYKAYVEAISIADKISDRFGFAEVLSDMATLHRRMGHHQEALDHFH
                     RSLKIFESLGNLVFQGVVLHGIGEVYLQEGRDTEALKYFRKALKIARQSKDLFGISFE
                     QDSIGDILFNSGKIADAKTQYLKNLKLQQKIGDNEGIAHTYGNLGNVARMEKNLPLAI
                     EYYTKNIKMTGEIGDKDGQGKGFYNLALVYEDLQDKEKAIQSLHKALKCFTQAGSVKL
                     IELTKQRLQENLQQNE"
BASE COUNT         1039 a          570 c          559 g          836 t
ORIGIN      
        1 tatagaaagc ccgctaaaag ctaaaatagg gctcacttat agcaatctct atgtgggagt
       61 tttaggagca aaacaacgct gtgaatttgg cattatcggc acaggagtta atgtttcagc
      121 ccggttaatg acttccgcta aatacgggca aattgttttt accaaggata ttctacccag
      181 tgtgcaaagc cgttttgaag tccaattttt gcgtaaggaa agggttaaag gcatcaaaga
      241 cgaattgttc ttttattgta tcctgcgtga attgccggaa tttttgaaca gctataaaag
      301 gcagtatcaa aataagaccc agatttgcta ccaggaacaa actgccgaaa tcattcaaaa
      361 aataaaggac aaaaaaatca atcaagtgct tatttccgga gatcatggca ccggaaaatc
      421 ctttctcagc tggcagatat taaacaattt ctacgaggaa aactacaaaa ttagcatctt
      481 cgtgttagat gaattcaacc atcacgatcc tttaatcctg catcgtaaat ttatcagcaa
      541 tgaactggaa ctgaatgatc ccttgtcgga acccgaaaaa ctgcaaaaat acctgggaga
      601 aattttggaa gaaagagatg cggatatcct tttaaacgct ttaggactgc aaaataaaga
      661 aactatctta accaatgaca gcggaaaaca aattgaattc ctcttgcttt ccctgcaaaa
      721 atccctggac ttgctgatgc gtgattttga ccttattttg ctggataata ttcaatggct
      781 ggatgatctc tcggctcaaa tcctccagaa acgcttggaa gatgacaagc ctaaatcaca
      841 aactcttatt ttaactacta ctaaagagat aacaaactac caaaacaagg caaatgccaa
      901 aacggaattt attagtttaa gggaattgaa tcgggaagaa gtaatagctc tgattcgctc
      961 tcaaatccct aatatcactt ttcaagcagt ggattatatc tacaatttgg caggaggaaa
     1021 tccgcggttt attactgagc tttgttccca aatactttcc agtttccccg atccagatat
     1081 gctgattacg gaatctaata tttatgatat tcaaaataag ggtcttttac cctatagcgt
     1141 agaaaatctt tttatcatga aatatgagtc attaagtcag gaagccaagg acatcctaaa
     1201 aaaggcatcc attataggta agggctttac cttgaacgaa atttttgaaa cccagagcgg
     1261 aataagccaa aatgaaataa tacccgtaat ttcagaactg caaaataatg aaattataga
     1321 tattacaacc ctctctccag aagttcaata tcttttcaat aatgctctta tgcgccaagc
     1381 tatctattct actattttgc ttggagaaaa ggtttctctc cataatcgca ttgcctcttt
     1441 ttatgaagaa aaatatggaa cactggcaaa ccgccacagt gaattacttg cctatcattt
     1501 tcatttggga gaaaataagg aaaaagctct gcattatgct ttaatagcag gcgaccaaaa
     1561 tcagaaaata aataaccaca gtgaggcaat ttattactat caaatagctc ttcagcatac
     1621 tacagaaaga ttggaaaaaa tagccatcat ccttagtatt gtggattccc agctctattt
     1681 aagcgaagtg gaagtggcaa aagaaaacct ggaaaccatt gaaccgaaag aaatttctct
     1741 tcccgaactc cttaaaaagt atcatttttt gcgctgcagg gtctattacc tgagcggtga
     1801 ctatgaaggt gttctgaaat atctgaaaaa tgtaaccgac tttgaaggca aatatggaga
     1861 acaaatacgc gtttatcaat tggactgcct ttatcggctt ttcctgattg aagatttttc
     1921 tgccctgcta aaggacttga aacaggaatt tgttcagcaa gcggcaaata ccctgaatgt
     1981 aaaatcccaa aagccatcca tttcaacttt gctttcccgt tttcagaaaa ttccggaaga
     2041 tgaaattaca gaggagcaaa aacactatct ctatctattg ctgaaactgg aatccataga
     2101 ggcaaatcat tttatcaata caggatatta tcaaaaagca ctaaaatcct tactttccca
     2161 atacgaactt gcaaaagctc tgaaagatga cctctcctta aggattgcca gtaaccgact
     2221 gggagttgtt tatacgcgaa tgggaaattt agattctgcc tataaagctt atgtggaagc
     2281 aattagcatt gccgataaaa taagtgaccg ttttggtttt gccgaagtcc tttccgatat
     2341 ggcaacttta catcggcgaa tgggacatca tcaggaagct ttagatcatt ttcatcgcag
     2401 cttgaaaatt tttgaatcct taggaaatct tgtctttcaa ggcgtcgtct tacacggaat
     2461 tggagaagtg tatttgcagg aaggacgcga tactgaagca ttgaaatatt ttcgtaaagc
     2521 cctaaaaatt gcccgccaaa gcaaagacct ctttggaatt tcttttgaac aggattccat
     2581 tggagatatt ttatttaatt ccggaaaaat agctgacgca aagacacaat atttgaaaaa
     2641 tctcaaatta caacaaaaaa ttggcgataa tgaaggaatt gcccatactt acggtaattt
     2701 aggcaatgta gccagaatgg aaaaaaacct tcctctggca attgagtatt acactaaaaa
     2761 tattaaaatg acaggtgaaa ttggcgataa agacggacaa ggaaaaggtt tttacaatct
     2821 tgccttggtt tatgaagacc tgcaggacaa ggaaaaagca atacaatccc tgcataaagc
     2881 tctaaaatgc tttacccagg caggttcggt taaactcatt gaactgacta aacaacgctt
     2941 acaggaaaat ttgcagcaaa acgagtgaag gatgaaaaaa gggctcacgc agatttcgca
     3001 gatg
//
LOCUS       DAOPFL010000144         2989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_45969_length_2989_cov_103.937628, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000144 DAOPFL010000000
VERSION     DAOPFL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45969_length_2989_cov_103.937628"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLG69_07215"
     CDS             complement(<1..416)
                     /locus_tag="PLG69_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPI43333.1"
                     /translation="MKLRRIFIALGVALIALFSLSCSPKQKKILRVGTDAENPPFTYQ
                     EGSEFKGIDIEICKRIADKLNMQLQITKYQFDELFSALALNKIDIAASAITITPKRKQ
                     TMDFTIPYFETDLAVVAKSNSPVKVEKIEDLGKYIVG"
     gene            complement(519..1301)
                     /locus_tag="PLG69_07220"
     CDS             complement(519..1301)
                     /locus_tag="PLG69_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425332.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPI43334.1"
                     /translation="MITALLVIICIAIAAGIIWLIFTKDKVVARTWGIVGLSFIIGLT
                     VFGYFFMQSRQAPTSLRVVGYASKLFESDLVKWNLTMQRNVSHDGLKDGYNKMSNDVN
                     AFKNYLEEQGIPEKDISIQPINSYQTTDNYGNPTGYALNQNVFIVSSDIPKIETLALK
                     PDFFAERGILLLNSNLAYLYTKLPDLKKQLLSEATKDAVARAEEIAGSGNTKLGKLRE
                     ARAGVFQITEPYSTEVSDYGIYNTSTRSKSISVTLTAIFKLK"
     gene            complement(1319..1753)
                     /gene="tsaE"
                     /locus_tag="PLG69_07225"
     CDS             complement(1319..1753)
                     /gene="tsaE"
                     /locus_tag="PLG69_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPI43335.1"
                     /translation="MKTLNLSTEQDTIDLAKYIAPLLKEGDIITLFGDLGSGKTFFVK
                     QLGKALGIEEEIDSPSFVLMKEYSGGRLTLYHLDLYRLKNKEEIYCLGLFDILEQGIT
                     VIEWPLLVNDLLPYQTFKLEFHFDGKKRWVDIIPDKEHSPYF"
     gene            complement(2229..>2989)
                     /locus_tag="PLG69_07230"
     CDS             complement(2229..>2989)
                     /locus_tag="PLG69_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HPI43336.1"
                     /translation="TPYSRNSIFSGLMPIDIAKRFPDYWVTSSEMDNSRNRNEHQLLD
                     EHIEELGFKLDPTSKYVKIFNMEEGNFVLRKIETWNKENLIVLVYNFLDLLAHHRSRD
                     QILQETIPNEEGLRAFTKHWFLHSSLYEALKLIAKQDAIVILSTDHGSIKVNRATQVI
                     GDKETSITVRYKEGKNLSVNERHALFVKKPGDFGLPSKSIVDNFIFAKDDYYFVYPNA
                     YHQYQKQFNGTFQHGGVSMEEMILPIATCRTKRR"
BASE COUNT          904 a          573 c          544 g          968 t
ORIGIN      
        1 cccacgatgt attttccgag gtcctctatc ttttctactt ttacaggaga attggatttg
       61 gctacaacag ccaggtctgt ttcaaaataa ggaatagtaa aatccatcgt ttgtttgcgt
      121 ttcggggtaa tagtaatagc agaggcagca atatcaattt tgttaagtgc taaagcagaa
      181 aacagttcat caaactggta tttggtaatt tgtaattgca tattcaattt atccgctatg
      241 cgtttacaga tttctatatc aatgcctttg aattcactac cttcttgata agtaaaaggt
      301 ggattttcag catcggttcc taccctcaat atcttctttt gtttcggaga acaagataaa
      361 gaaaataagg caatcagtgc gacacccaaa gcaataaata tcctgcgtaa tttcatcttc
      421 ttacctttac tatatggttt tatataaatt ataatccccc tcggataaat tacaagtatt
      481 ttatccgtaa aattacaagc aggtttgtgg aaaaaaatct actttaactt aaaaatagca
      541 gttaaagtaa cggaaatgct tttactgcga gtgctggtgt tataaatacc atagtccgat
      601 acttctgttg aatagggttc tgttatttga aaaacacctg cacgcgcctc tcttaatttg
      661 cccagtttgg tatttccgct accggcaatt tcttctgcac gagcaactgc atctttggtt
      721 gcttcggaaa gcaactgttt tttcaaatcc ggcagtttag tataaagata tgccaggtta
      781 gaatttaaca ataagattcc gcgttccgca aaaaaatctg gttttaatgc caaggtctca
      841 attttgggta tatctgaaga cactatgaaa acattttgat ttagagcata gccagtagga
      901 ttaccatagt tatcggtggt ttgataactg tttatgggct ggatactgat atccttttcc
      961 ggaattccct gttcttcaag ataattcttg aaagcattta catcattgct cattttgttg
     1021 tagccatctt tcaaaccatc atggcttaca ttgcgttgca tagtcagatt ccattttacc
     1081 aaatcggatt caaagagttt gctggcatat ccaactacac gtaaactggt tggtgcctga
     1141 cgactttgca taaagaaata gccaaaaacc gtcaaaccga tgataaagga taaaccaaca
     1201 attccccagg tgcgggcaac tactttatcc ttggtgaaaa taagccagat tatacctgcc
     1261 gcaattgcga tacagatgat tactaatagt gctgtgatca ttttttgctc cttaaaactt
     1321 aaaaatatgg agaatgctct ttatcgggaa taatatccac ccagcgtttt tttccatcaa
     1381 agtggaattc cagtttaaat gtctgatagg gtaaaagatc atttaccaaa agaggccatt
     1441 caataacggt tattccttgt tccaagatat caaacaaacc caggcaatat atttcttctt
     1501 tatttttcag acggtatagg tctaaatgat acagggtaag gcgtccaccg gaatattcct
     1561 tcattaaaac aaaagaagga ctgtctattt cttcttcaat tcccagtgcc ttgccaagct
     1621 gttttacaaa gaaggttttg ccgctaccca aatcgccaaa aagagttata atatcccctt
     1681 cctttaataa cggtgcaatg tattttgcaa ggtctatagt gtcctgctca gtagataagt
     1741 ttagggtttt catctcagct taaaaagaat gataaatgaa tatagtgaga tggagatttt
     1801 gtcattcctg cgaaagcaag aatcaaaata tctgcaattc ccctatcaaa cctatcggaa
     1861 aagagaaaag aaaaagtttg ccaaggtctt accttagggt aacggcattt tgatagggaa
     1921 agcatacggt taataaacat tttctgtgta tcccaccttt aatctctttt ctcttcgtca
     1981 ggggttatat atttttacac ctaaatcacg gaggacagtg ttcctctctg aaaaagaaag
     2041 atttggaatt tgcggagctc cggctataca atttttctgc acttctgact tcattttaca
     2101 ttttcttcct ttcccgtcaa gagtcctcat cgctaaacta atttgagata agggttaaag
     2161 atatataagc gatgaggact cctgacttcc ttcattctat attctacatt ctttctcttt
     2221 ataaacaact atcttctttt agtgcggcag gtagcaatcg gcagaatcat ttcttccata
     2281 gagaccccac cgtgttgaaa agtgccgtta aactgtttct gatattgatg ataggcattg
     2341 ggatacacga aatagtaatc atccttggca aagataaagt tatccacaat gcttttggag
     2401 ggcagtccaa agtccccggg tttttttaca aacagagcgt ggcgttcatt cacggaaaga
     2461 ttttttcctt ctttataacg gactgttatg gatgtttctt tatcaccgat tacttgagtt
     2521 gcacggttca cttttataga accgtggtct gtagaaagaa ttacaatcgc atcttgtttg
     2581 gcaatcagtt tcaaagcttc atagagcgaa gaatgcaaaa accagtgttt ggtaaaagca
     2641 cgcagacctt cttcattggg aattgtttcc tgtaaaattt gatcccgact gcgatgatga
     2701 gctaacagat caaggaagtt ataaacaaga acaatgagat tttccttgtt ccaagtttca
     2761 attttgcgca gcacaaaatt gccttcttcc atattgaaga tttttacata cttgctggta
     2821 ggatcaagtt taaagccaag ctcttcaata tgttcatcca gcaattgatg ttcattgcga
     2881 ttacggctgt tatccatttc actggaagtt acccaataat cgggaaagcg tttagcaata
     2941 tcaataggca ttaaaccgct gaaaatggaa tttctgctat agggagtag
//
LOCUS       DAOPFL010000145         2972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_46348_length_2972_cov_94.011313, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000145 DAOPFL010000000
VERSION     DAOPFL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46348_length_2972_cov_94.011313"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLG69_07235"
     CDS             complement(<1..878)
                     /locus_tag="PLG69_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPI43337.1"
                     /translation="MLENENNQLPVQEEIKLSDYFRVILQYRYLVILVFLVALVATVY
                     YTARLPKIYSASTRILLEDANKQSDLMFLSTGGLGKNTLNNQIELIRSKPIMNLAWEI
                     MKKYPDWDTFPASQAADPASTLGRMKVESKRETDVLTISFESTNPTEAMAAVNSIAEA
                     IQQQNTQYARLEFTTIREFLETQLDAISRRLQSSENDLREFKNLNKLTQLSEETNKLI
                     EQASEVEAEYEAALTDQAVKAKSLDLLNRQLQEQDSLLVNVDNIIKTPYINELRKQIV
                     DTQSLITKLVTKNEYP"
     gene            complement(891..1406)
                     /locus_tag="PLG69_07240"
     CDS             complement(891..1406)
                     /locus_tag="PLG69_07240"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPI43338.1"
                     /translation="MLRKLNREDNVKRLLLVFLLISFSLVLFSQQTINFTPSSNISAY
                     TYEGIRSGVEKLKMNNYILGQVYKPGLYTVPDDTDFLTLLSLAGGPKEDAKLTKIRIV
                     RPSSEGDKVIWVNFKKYLETGDPTLIPELKPGDTIIVSGTIFYAVSKVAKFLSDITIA
                     LGVYNIITGFK"
     gene            complement(1499..2278)
                     /locus_tag="PLG69_07245"
     CDS             complement(1499..2278)
                     /locus_tag="PLG69_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43339.1"
                     /translation="MRKIIILTTLLLLVLLFSCSGDDPPEKDKIPPTTPKLISHLGDT
                     GDDPIIVNGVTVSLNDDNNGIDTVPDGNWIKVPWEKFVDNDLSHVKVYRYSESDPEPI
                     LINTVPASEDYYLDQSSLVEREWYYYYIELYDASGNFSVSDTVSYAILAKSILSSPVE
                     GETINQDTAKLCWERGDSQTNKFRILLWDNLTGNLVFSYDYYYTPNEESPSPPEFPFP
                     RLNPRPPSGQVFRWRIDAFDDDPEHNLEMGSESKERTLIIQ"
     gene            complement(2290..>2972)
                     /locus_tag="PLG69_07250"
     CDS             complement(2290..>2972)
                     /locus_tag="PLG69_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43340.1"
                     /translation="HFDEKYLIGTNVDQRINNSLYHLPGVPDSKFSSYDDLYQFAFSK
                     KIHYDANMGWLFFAVPFDFGFGGTVKLIKRSIWDRTGTGTGLDIGFKAKTDLAVIFDW
                     EELGDLHFGVNFQDIGGTDITWDTPSKISDEVLFNTKTGLAVVQPLPKLKSVVTLAYD
                     YDYVYGGTNHFGVDWEYDKKGNLRLGYYDNSFSAGATIKLYGVMLDYALITNPVGISN
                     RLGLRVRF"
BASE COUNT          907 a          571 c          561 g          933 t
ORIGIN      
        1 aagggatatt cattttttgt aactaatttg gtgatgaggg attgagtatc tacaatctgc
       61 tttctcagtt cattgatata aggtgtttta ataatattat ccacattcac cagcagagaa
      121 tcctgttcct gtaattgacg gtttagcaaa tccagggatt tggcttttac tgcctgatca
      181 gttaacgctg cttcatattc agcttctacc tctgatgctt gttcaataag tttattcgtt
      241 tcctcggata attgagtaag cttatttagg ttcttaaatt cccgtaaatc attttcagaa
      301 ctttgcagac gacgggaaat agcatctaat tgagtttcca gaaattcacg gatagtagta
      361 aattccaatc gagcatattg agtattttgt tgttgaattg cttccgcgat ggaatttaca
      421 gcagccattg cttcagtagg attagtagat tcaaaactaa tggtgagaac atcagtttcc
      481 cgttttgatt ctactttcat tctacctaaa gtggaagccg gatcagctgc ctgacttgca
      541 ggaaaagtat cccaatcggg atattttttc ataatttccc aggcaagatt cattatgggt
      601 ttgctgcgaa taagttcaat ctggttattt aaagtgtttt tccctaaacc gcctgtagaa
      661 agaaacatca ggtcggattg cttatttgcg tcttccaata aaatgcgggt agaagcagaa
      721 taaattttag gtaaacgggc tgtgtaatat accgtagcta ctaatgctac aaggaatacc
      781 agaattacca gataacgata ttgcaggatt actctaaaat aatcggaaag ttttatttcc
      841 tcttggacag ggagttgatt attttcattt tccagcattg aataccctcc ttatttaaat
      901 ccagttatga tattataaac gcccagagct atagtgatgt cagagaggaa ttttgccact
      961 ttagatacgg cgtagaagat ggttcctgat acaatgattg tgtcccctgg ttttaattcc
     1021 ggaataaggg ttggatcacc tgtctccaga tatttcttaa aatttaccca gataaccttg
     1081 tcaccttcgg aagaaggacg cacaattctt attttagtaa gtttggcatc ttctttagga
     1141 cctccagcca aggaaagcag ggttaaaaaa tcggtatcgt caggaacagt atataagccg
     1201 ggtttataga cctgacccaa gatataatta ttcattttca gcttttccac cccgctgcgg
     1261 ataccttcat aagtataagc agaaatattt gaagacgggg taaaattaat cgtctgctgg
     1321 ctgaataaaa ccagggaaaa gctgatgagt agaaacacta acagtaatct cttcacatta
     1381 tcctctctat ttaatttgcg taacattctg aacatcgctg ttcaagtcaa gatttttctg
     1441 taacttcatt atgtccagac cggtttttat atgctttttc aaggttggac tgttatccct
     1501 attgaattat caaggttcgc tctttcgact cggaacccat ttccaaatta tgttccggat
     1561 cgtcatcaaa agcatctatt cgccaacgaa aaacctggcc acttggaggt cgtggattta
     1621 aacgaggaaa aggaaattct ggtggtgaag gagattcttc attaggagta taataataat
     1681 cgtaagaaaa aaccagatta ccggttagat tatcccaaag caggatacga aatttgttgg
     1741 tttggctatc gcctctttcc caacataatt ttgctgtgtc ctgattaata gtttccccct
     1801 caactggaga actgagaata cttttagcta aaatagcgta ggaaacggtg tccgaaacgc
     1861 taaaattgcc gctggcatcg tagagttcaa tataatagta ataccactct ctttcaacca
     1921 atgaactttg atccagatag taatcttccg aagctgggac agtattaatt aatattggtt
     1981 cagggtcgga ttcggaatag cggtagacct ttacatggct taaatcatta tctacgaatt
     2041 tttcccaggg aacttttatc cagttgccat cgggaacagt atcaatgcca ttattatcat
     2101 cgtttaggga aactgttact ccatttacaa taatgggatc atcacccgta tctccaaggt
     2161 gtgaaattag tttaggtgta gtaggaggaa ttttatcttt ttcgggagga tcatctcctg
     2221 aacaagaaaa gagcaaaacg agcaataaga gtgtagttaa gataattatc ttgcgcattt
     2281 ttattctcct taaaatctaa cccgtaatcc caaacggtta cttatgccga caggattcgt
     2341 gattagagca taatcaagca taacgccgta aagttttatt gtagcacctg cactgaaact
     2401 attatcataa taaccaagac gcaaattacc ctttttatca tattcccagt ccacaccaaa
     2461 atggttagtt cctccataca catagtcata atcataagcc aaggtaacaa cggatttcag
     2521 tttaggtaag ggttgaacta ctgctaagcc ggttttagta ttaaagagaa cttcgtcact
     2581 aattttactg ggagtatccc aagtgatatc tgttccacca atatcttgaa aattgacacc
     2641 gaaatgtaaa tctcccagtt cttcccaatc gaaaataacg gcaaggtcgg tctttgcttt
     2701 aaaaccaatg tcaagtccgg ttcctgttcc ggttctatcc catatagacc ttttaataag
     2761 tttcaccgtt cctccaaaac cgaaatcaaa aggaacggca aaaaacaacc aacccatatt
     2821 ggcatcataa tgaattttct tggaaaaagc gaattgatag agatcatcat agctggaaaa
     2881 tttgctatcg ggaacaccag gtaaatgata taagctgtta tttatccgct ggtctacatt
     2941 agtgccgatg agatactttt catcaaaatg gg
//
LOCUS       DAOPFL010000146         2935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_47179_length_2935_cov_135.743750, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000146 DAOPFL010000000
VERSION     DAOPFL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47179_length_2935_cov_135.743750"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1163
                     /locus_tag="PLG69_07255"
     CDS             45..1163
                     /locus_tag="PLG69_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43341.1"
                     /translation="MRKLLTIILLGITLLFSFAYLDAAVKVKVKGTVETKRKKPKPEE
                     IKTATYNAKILAIKKYASEKDSRRRELINELLPEIEKNIDNYILEVNYLNDGEYKNGI
                     WEQLMEITIDDVQLDLLINERMKGIMEMTKPTYLSFVYVAREVAAVESEGKTVKKDLS
                     AKSDVAASSSVATSGETSSDIYDSDKISTSSQADISLSKKGSTNKSIMYSESVTNKSE
                     GITYRIYNPGEIDTKVTEVFNKANFEVVPTYEVGILPEKFAYDFAALNEISSSTQKEA
                     TDIAREAGLDFLAVAMLDVGREEIDPVTGGSKVYVKVNGYILDLRKKFAVKICSAGPY
                     QFSGLGSNPTVAKTNALIEAATHASQDLVDQLRVKLGS"
     gene            1177..1896
                     /locus_tag="PLG69_07260"
     CDS             1177..1896
                     /locus_tag="PLG69_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43342.1"
                     /translation="MGTIQRLIVLICIFLLLVSCVGLEAKKKKADPEVKLFLDDSQLD
                     NPEYYNKLPLLSLEAFASALLMPKTSNFKVINYNYKLAKAESKIAEEMVAVSLGMRQE
                     YVKIEAEGKSLMSANLEGSDAEISETLKAKLEDLKTDKAQLSESQKVYFLSGFIYLAS
                     SIVREKTILESSKKFVNEAKNAKGIDAVMYVKDIPYATNVVIGLPVLLSNQMRTISEF
                     ITIAKTQNIEIPKDVTDSLFQ"
     gene            1995..>2935
                     /locus_tag="PLG69_07265"
     CDS             1995..>2935
                     /locus_tag="PLG69_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPI43343.1"
                     /translation="MPKKIIYTVLLFLLIGSCLYAGKKKPEWISNPYSQYPSKQYLCG
                     VGIAKELNIAQNEALKNIAGFFETRVIAVTKSMESEKSSSSNYQTSSETSSSVKAIAE
                     GTLKYTQIKETWQDPKTKTFYALAVMDKIAVQTIYRNEIDDCEEFIENCLAPTDDPLI
                     RFAKILSAIDKLQQTQNAYTYYNALASDSGILLKPFHSLQELLELRNYAAREIPLQIR
                     VFGDSSGLLDASLKNVINQIGLLENDNPEYQIVATMNPEPHKSLGQQFFQPLRLNISF
                     NYGEKNLFSFVDKTEQGGSTIEEATSRGIRVLAQNIE"
BASE COUNT          985 a          533 c          587 g          830 t
ORIGIN      
        1 acgctgaaag aagataaaaa actaatgaag gggatattaa aaaaatgcgc aaactactaa
       61 ctatcatttt gctcggcatt actttgctct tttcctttgc ttatctggat gcggcagtta
      121 aagttaaggt gaaaggcacg gttgaaacca aaaggaaaaa gccgaaaccg gaagaaatta
      181 aaaccgctac ttataatgct aaaattctgg ctattaagaa atacgcttcc gaaaaggaca
      241 gtcgtagaag agaactcatc aatgaattgc tgccggaaat tgaaaaaaac atagataact
      301 atattctgga agttaattat ctgaacgatg gtgaatacaa aaatggcatc tgggaacaat
      361 tgatggagat tactattgac gatgttcaat tagacctttt aatcaatgaa cggatgaaag
      421 gcattatgga aatgaccaaa cctacttatt taagctttgt gtatgtagcc agagaagttg
      481 ctgcagtgga aagtgaaggg aaaactgtta aaaaagacct ttctgccaaa tccgatgttg
      541 ctgcttccag ttctgttgca accagcggag aaacctcctc cgatatttat gatagcgata
      601 aaataagcac aagcagtcaa gcggatataa gcttaagtaa aaagggctcc accaataaat
      661 ccataatgta ttccgaatcc gttaccaata aaagcgaagg gattacctac cgcatttata
      721 accccgggga aattgatacc aaggtaacgg aggtctttaa taaagccaat tttgaagttg
      781 ttcctacata cgaagtgggt attttgccgg aaaaatttgc ctacgatttt gctgctctaa
      841 acgaaatttc ctcttccacc caaaaagaag caacagatat tgctcgcgaa gccggtttgg
      901 attttttggc tgttgctatg ttggatgttg gacgggaaga aattgatccg gtaacaggag
      961 gcagtaaggt ctatgtgaaa gtaaacggct atattttaga cctgcgaaaa aaattcgctg
     1021 ttaaaatttg cagtgcagga ccttatcaat tcagcggttt gggctcaaat cctaccgtag
     1081 ctaaaacgaa tgctttaatt gaagcggcaa cacacgcctc acaggatttg gtagatcagc
     1141 ttcgtgtaaa acttggaagc taaaataaga aatttaatgg gaactattca gcgattgatt
     1201 gtcctcatct gtattttttt gcttctggta agctgtgtag gactggaagc gaaaaagaaa
     1261 aaagcggatc cggaagtaaa attgtttttg gatgattctc aattggataa tccggaatat
     1321 tataacaaac tgcctttgct ttcactggaa gcatttgcca gcgctctttt gatgcccaaa
     1381 acatcaaact ttaaagtaat taattataac tataaactgg ctaaggcgga atccaaaata
     1441 gcggaagaaa tggtagctgt ttctttgggg atgcgtcagg aatatgtaaa aatagaagcg
     1501 gaaggaaaat ctctgatgtc cgcaaacctg gaaggcagtg atgccgaaat atctgaaacc
     1561 ttgaaagcca aactggaaga cctgaagacg gataaagccc aattgagcga atcgcaaaag
     1621 gtctattttt taagtggctt tatttatctt gccagctcta ttgtgcggga aaaaaccatt
     1681 ctggaaagtt ccaaaaagtt cgtaaatgaa gccaaaaatg ctaaaggaat agacgcagtt
     1741 atgtatgtta aagatattcc ttatgctacg aatgtagtaa ttggtcttcc tgttttgctt
     1801 tcaaatcaga tgaggactat tagtgagttt attaccattg ccaaaaccca aaatattgaa
     1861 attccgaaag atgttaccga ttccctgttt cagtgaattg tctattttgt ttatgggtgc
     1921 aacaacaaat ttgagttaga tgaatagttc aagttcttca ttccgcttaa attgcttaaa
     1981 ggttagatta agtaatgcct aaaaaaatta tttataccgt ccttttattc ttgcttatcg
     2041 gttcctgcct ctatgccgga aagaaaaaac cggaatggat ttctaatcct tattcccaat
     2101 atccttccaa gcaatatctt tgtggcgtgg gaatagcaaa agaactgaat attgcacaaa
     2161 acgaggcatt gaaaaatatt gccggctttt ttgaaacacg cgttattgca gttacgaaaa
     2221 gtatggaaag cgaaaaaagc tcttcttcca attatcaaac aagcagcgag acctcttctt
     2281 ccgtaaaagc aatcgcagaa ggaactttga aatatactca gataaaagaa acctggcaag
     2341 accctaaaac caaaactttc tatgccttag cggtaatgga taaaattgcc gtgcagacca
     2401 tttatcgcaa tgaaattgac gactgtgagg agtttattga aaattgcctt gctcccactg
     2461 atgatcccct tatccgcttt gctaaaattt taagcgctat tgataaattg caacaaacac
     2521 aaaatgctta tacttattat aacgctctgg caagtgattc aggcattttg ttaaaacctt
     2581 ttcacagctt acaggaactt ttggaactca ggaattatgc ggctcgggaa attcccctgc
     2641 aaatcagagt ttttggcgat tcttcaggcc ttttagatgc ctcgcttaaa aatgtaatta
     2701 atcaaattgg cttgttggaa aacgataacc ctgaatatca aattgtcgct acaatgaatc
     2761 ccgagccgca taaatccctg ggacaacaat tttttcaacc tctgcgtctg aatatttctt
     2821 tcaattacgg tgaaaaaaac ctcttttcct tcgtagataa aacggaacaa ggcggttcta
     2881 ctattgagga agcaacctcc agaggaatta gagttcttgc ccaaaatata gaaaa
//
LOCUS       DAOPFL010000147         2930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_47308_length_2930_cov_81.649739, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000147 DAOPFL010000000
VERSION     DAOPFL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47308_length_2930_cov_81.649739"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1412..1750
                     /locus_tag="PLG69_07270"
     CDS             1412..1750
                     /locus_tag="PLG69_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HPI43344.1"
                     /translation="MKKLLIVIIALALIFILAGCAKGPESVAKKFVSAWKSQDMKTME
                     KLSTPESKEMIGMFSMFKVKDATLGETKVTGDSATVNVKYTTEDGKQDEFDLNLVKQG
                     SKWLVEIKGK"
     gene            1750..2382
                     /locus_tag="PLG69_07275"
     CDS             1750..2382
                     /locus_tag="PLG69_07275"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HPI43345.1"
                     /translation="MSFRDLTLLGLTSLIILLTGSSAISSSRTANLSLNEKCEFPPLS
                     SLQLKDGDVILRRGNSIFSELIARNFPASEGMSHCGFIFKIDGQYQVIHTISKSLSDI
                     DGIRINTLEKFVREAKQNRICIIRYHKKLNSDSMKERCLALLKQKIPFDNDFDLNDSS
                     KLYCSELLRYVYREQGEPDFFEMRKIVGVSVIDFSTFFNTELFIPVYKNY"
     gene            2558..2632
                     /locus_tag="PLG69_07280"
     tRNA            2558..2632
                     /locus_tag="PLG69_07280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2590..2592,aa:Thr,seq:tgt)
     gene            2647..2732
                     /locus_tag="PLG69_07285"
     tRNA            2647..2732
                     /locus_tag="PLG69_07285"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2681..2683,aa:Tyr,seq:gta)
     gene            2758..2834
                     /locus_tag="PLG69_07290"
     tRNA            2758..2834
                     /locus_tag="PLG69_07290"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2792..2794,aa:Gly,seq:tcc)
     gene            2851..2926
                     /locus_tag="PLG69_07295"
     tRNA            2851..2926
                     /locus_tag="PLG69_07295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2884..2886,aa:Thr,seq:ggt)
BASE COUNT          981 a          496 c          524 g          929 t
ORIGIN      
        1 aatgccaatt ttgtgccttt atttgcatag atatgttttt gtcaagcttg gaaactacac
       61 gccattaatt tattttgctt ggggctaaaa aggttgaagg gatagagaag attgagaaaa
      121 ttttgactca caattttcac ctgccttcca gaaaaccaca atctacgaac tatttcactt
      181 acaaccgcac tataactggt ttccaaattg aaaaattgtg agttaaaaga gaaaacaagc
      241 acacttacaa ccgagctata acaatcccac aaattataag gtaatgaagg aatctatatt
      301 tacacgataa aaggaaaaga aaaaatatcc tttttttgaa ataataacat ttacgactgc
      361 acaattagat tctttacgct gtattatact gcggatacca ataaaaacaa cctgatcatc
      421 ctatatctgc tttttaattt tagttataag aataatagag ttaaatcctt ttctggatgt
      481 tatataaata tattttgcct atcaaagtag ctttcctgtc tattatcttg tatttcttat
      541 cttggtataa tatttttatt tcccctatta atttcaagga gctgtcccga aattaaaagt
      601 tagttttggg acagccccat atttatctaa ctgccatctc tactggattt tttcctatta
      661 ttagtagttt atgaatataa atattatata aaatatggat ttaccgatac aatatttcat
      721 tcttctacgg aaattctcta actcctgcta aacaaatata attacatctt cttccttaat
      781 tgcattgctt acccattgct tacccattgc taatgccatc cgtaatgggt aagtaatgct
      841 tcagcaattg aggcaaaaaa aaatgagtta tcctgaaaat agtaaacgat attggaaaaa
      901 gaaatccgat tgatccagca gatcaagcta tctgcgttct attgccatta tgatttgtag
      961 gcaccgtaaa agttatttcc cagttcagaa ttttgaagaa gccaatgact tcttacttat
     1021 gtgcattggg atcaagaata aggttggtag atatcttcct ttttacgagg ggattacaca
     1081 cccagagcta taattgtttc gctcgtcgga ctattagacc aaataataca ggtgaggata
     1141 acagcaagtt ggtaaattgt ttacttgctt ttgataacag aattcgcaaa agatacttta
     1201 ttctttgttt ttttgaatct aaattgggca aacttaaggt ctaattataa ttactaattg
     1261 agaatactat tttatttgga ttaattaaaa ggggttataa ataaacagaa ggtaattaat
     1321 gtaggagaaa aatcatataa tgaaaaaaat acttgacaag acaagaatat taaaaataaa
     1381 gtaaatcata aaaaattgtt aaggagtaac aatgaaaaaa ttactgattg taatcatcgc
     1441 ccttgccctt atttttatcc ttgcgggctg tgcaaaaggt cccgaaagcg ttgccaagaa
     1501 gtttgtttct gcatggaaga gccaagatat gaaaacgatg gaaaagctct ccactccaga
     1561 aagcaaagag atgattggca tgttctctat gtttaaggtt aaagatgcaa ccttaggtga
     1621 aacaaaggta actggagaca gtgctaccgt caatgtaaaa tatactactg aagatggaaa
     1681 acaagatgaa tttgatctga atctggtaaa acaaggcagt aaatggttgg tagaaattaa
     1741 aggaaaataa tgtccttcag agaccttacc ctattaggtc ttacatcact gataatttta
     1801 ttaactggca gttcggcaat ttcttcaagt cggactgcca atttatcctt gaatgagaag
     1861 tgtgaatttc ctcctctatc ctcactgcaa ctgaaggatg gagatgtaat tctcagaagg
     1921 ggtaatagca tattctcaga acttattgcc agaaattttc ctgcttctga aggtatgtct
     1981 cattgcggtt ttatttttaa gattgatggt caatatcagg taattcatac tatttccaaa
     2041 tctctttccg acatagatgg catacgcatc aacacattgg aaaaatttgt acgtgaggca
     2101 aaacaaaatc gtatttgcat tattcgttat cataaaaaac ttaattctga ttcaatgaaa
     2161 gaaagatgcc tcgcactttt aaaacaaaaa atcccttttg ataacgattt tgatctcaat
     2221 gactcttcta aattgtattg ttcggaactt ttgcgctatg tttatcggga acagggagaa
     2281 ccggatttct ttgaaatgcg taaaattgtt ggtgtttctg tgattgattt ttctactttt
     2341 tttaacacag agctttttat ccccgtttat aaaaactact aatttcttca aatacttcaa
     2401 ccctaatagc tattaaccat atacaaggtt aaaagtgttg acagaaacag agggcaaaat
     2461 ttgttagcgc caagtatgaa agacaataag aatatagagt gatacaaaaa agcagttgac
     2521 aaaaaaaggg tgttaaaact tgatgacaaa acttgctgcc gatgtagctc aaaggtagag
     2581 caattgattt gtaatcaata ggttgggggt tcaattccct tcatcggctc cagaaattat
     2641 agtcgtggag aggttcccga gtggtcaaag ggatcagact gtaaatctgc tgtcagatga
     2701 cttcggaggt tcgaatcctc ccctctccac cagtagattt tagatataaa aagaaaagcg
     2761 ggagtagctc agttggctag agcatcagcc ttccaagctg agggtcgcgg gttcgaaccc
     2821 cgtttcccgc tccataattt tttagtgaaa gctcaagtag ctcagcaggt agagcacctc
     2881 cttggtaagg aggaggtcac cggttcaagt ccggtcttga gctccacttt
//
LOCUS       DAOPFL010000148         2923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_47467_length_2923_cov_115.845188, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000148 DAOPFL010000000
VERSION     DAOPFL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47467_length_2923_cov_115.845188"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLG69_07300"
     CDS             complement(<1..884)
                     /locus_tag="PLG69_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPI43346.1"
                     /translation="MPSKPNQFIQLRKEKLAKIKALGIDPYPIKSYRTHYISDLLKDK
                     EEQLQKAEEVTITGRLTAMRRQGKIGFGDLEDISGKIQLYVAQNLIGEENYELFKLCD
                     PGDFIQATGKTFFTQTGEYSLKCNNIKLLSKNIRPLPAVKEKIVDGKKIRYDEFSDIE
                     LRYRKRYLDLLLNPEHRKVFETRAKIITAIRNFMDSRGYVEVETPILQPLYGGANARP
                     FITHHNTLDMDLYLRIATELYLKRLIVGGFEKVYELGKDFRNEGMDRTHNPEFTMIEF
                     YEAYSDLEGMMDIAEELI"
     gene            complement(894..2405)
                     /locus_tag="PLG69_07305"
     CDS             complement(894..2405)
                     /locus_tag="PLG69_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPI43347.1"
                     /translation="MSDKLHTTSSKAAMKEMKEEYLRMLEESFQNTAEIKKGDVIEAP
                     IVSIGDQYIILNLGGKFDAYAEIGEFSDSRGVLPYKVGDNLKGYVVDQNENGYVVARS
                     LTKQYVDKQSILEAFEKKIPVQGKIYTPTKGGFNVDILGARAFCPVSQVSLHPVEDSS
                     QFIGKTLDFLVIECSENCRRIVVSHRQLEEMREQERKEMALAKLKVGDIVKGKVLRMT
                     TFGAFIDLGGIEGLMHVSEISWQHIVRPQDELKKGQEIEVKIMDIKGEKIALSRKVLL
                     EDPFEVAMKELHEGDIINCRVLRLHNFGAFAELKPGVEGLIPISEMSRNRNISHPRDI
                     VKEGDWVQVQILRIDEDTHKISLSLKALEADPWDKIDEIIQLETPFEGIVESSTNFGV
                     FVTISEGITGLLPKSRVRKNDTFQTGEPVTLMVTAIDKENHRITLDYTDRSPVELAEE
                     RVRSEEHNYRDQHNEKIRSSNQRRGGRNRSDEDWRKYANQKLNPAADNPFKDL"
     gene            2662..>2923
                     /locus_tag="PLG69_07310"
     CDS             2662..>2923
                     /locus_tag="PLG69_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPI43348.1"
                     /translation="MKNTYPELDKILAKYQDKKGALIPLLQEVQKEKGYLSRETMQYL
                     ADKMKIPPAEIFGVATFYSMFRLKPQGKHLIRVCKGTACHVSD"
BASE COUNT          843 a          604 c          526 g          950 t
ORIGIN      
        1 ttaatcagct cttcagcgat atccatcatc ccttccaggt cggaatatgc ttcgtaaaac
       61 tcaatcatcg taaattccgg attatgagtt ctatccatac cttcattgcg aaaatccttg
      121 ccgagctcgt aaactttttc aaagccgcct acaattaaac gctttaaata aagctccgta
      181 gcaattctga gatacaaatc catatccaaa gtgttatgat gagtaatgaa aggtctggca
      241 tttgctccac cgtaaagggg ttgtaaaata ggagtttcta cttccacata accccggcta
      301 tccataaaat tccgaatagc ggtaatgatt tttgcacgcg tttcaaaaac ttttcggtgt
      361 tcaggattga gcaaaaggtc aagatagcgt tttctataac ggagttcaat atcggaaaat
      421 tcatcgtaac ggattttttt gccgtccacg attttttcct tcactgcagg taagggtctg
      481 atgtttttgg aaaggagttt tatattattg cattttaggg aatactcacc ggtttgcgtg
      541 aaaaatgttt ttcctgtggc ttgaataaaa tcgcccggat cacaaagttt gaatagttcg
      601 tagttttcct cgccgattaa attttgagct acataaagct gaatttttcc ggaaatatct
      661 tccagatcgc caaaaccgat tttaccctgc cgtcgcatag ctgttaaacg cccggtaata
      721 gtgacttctt cagctttttg aagctgttct tccttatctt taagcaggtc agaaatataa
      781 tgagtacgat aggattttat gggataagga tcaataccca gggctttaat ttttgccagt
      841 ttttctttac gcaactggat aaactgattg ggtttgcttg gcattacgga aaattaaagg
      901 tctttgaaag gattatcagc tgcgggattt agtttctgat tggcatactt acgccaatcc
      961 tcatcactgc gatttcttcc accccggcgt tgatttgaac tgcgaatttt ttcgttatgc
     1021 tggtctctat agttatgttc ttccgaacgg acgcgttctt cagccaattc tacaggtgaa
     1081 cgatcggtat aatcaagagt aattctatga ttttctttat ctatggcagt taccatcaaa
     1141 gttaccggct cgcctgtttg aaaggtgtca tttttccgta cccgggattt gggtaacaga
     1201 cctgtaattc cttcactgat agtaacaaaa acgccgaaat tggtagagct ttccacaatt
     1261 ccttcaaaag gggtttccag ctgaataatt tcatctattt tatcccaggg gtctgcttcc
     1321 aatgccttca gcgaaagaga tatcttatga gtatcttcat ctatgcgtaa aatttgaact
     1381 tgaacccagt ccccttcttt cacaatatcc cggggatgag aaatgttgcg attacggctc
     1441 atttcagaaa taggaataag accttcaaca ccgggtttca attcggcaaa agctccaaaa
     1501 ttatgtaaac ggagaacacg acaattgatg atatctcctt catgcagttc tttcattgct
     1561 acttcaaagg ggtcttcaag caagacctta cgcgaaagag caattttttc tcccttgata
     1621 tccataattt taacctcaat ttcttgacct tttttcagtt cgtcttgcgg tctaacaata
     1681 tgctgccagg aaatttccga aacatgcatt aaaccttcaa ttccgcccaa gtcaataaaa
     1741 gctccgaaag tagtcattct taagacctta cctttaacga tatcacccac tttcaatttg
     1801 gctaaagcca tttcttttct ttcctgttct cgcatttctt ccagttgacg atgcgaaaca
     1861 actatccgcc tgcaattttc ggaacattca ataaccagaa aatcaagggt tttacctata
     1921 aactgagaac tatcttccac aggatgcaat gagacctgtg atacgggaca aaatgcccgt
     1981 gctccaagaa tatctacatt gaaaccaccc ttcgttggag tatatatttt tccctgcacc
     2041 gggatttttt tctcaaatgc ctcaagaatg gattgtttat ctacatactg tttagtcaag
     2101 ctccgggcaa cgacatagcc attttcattc tggtcaacaa cataaccctt taagttgtca
     2161 cctactttgt aaggcaaaac tcctctggaa tcggaaaact ctccgatttc ggcataagca
     2221 tcaaatttac cacctaaatt aaggattatg tattggtcgc cgatgcttac aatgggagct
     2281 tcaataacat cgcccttttt aatttctgct gtgttttgaa aggattcttc aagcatccgc
     2341 agatattctt ctttcatttc tttcatagca gctttgctgc ttgtagtatg taacttgtct
     2401 gacataagat tctcctaaag gcatttcaga gaaaccaaga tttagagtgg ctgttttttg
     2461 taaagatttt tatttattca gctttgaaca ggagataaaa agaagctgag aattacgatt
     2521 ttcagtgatg gtttttcaaa ctgtaaacca gttttacctt ttttgtttga catttcttgc
     2581 cttataaaaa tctgttgcct gtaaaaatga gaaaatatac tgtttatata tcttacaata
     2641 ttcacagata aaggactgac aatgaaaaat acttacccgg aattggacaa aattttggct
     2701 aaatatcagg acaagaaagg tgctctgata ccccttctgc aagaagtcca aaaagaaaag
     2761 ggttatttat ccagagaaac aatgcaatat ttagcggata aaatgaaaat acctcctgcg
     2821 gaaatatttg gtgtagccac cttttattct atgtttcgct taaaaccgca gggaaaacat
     2881 cttattcgtg tttgtaaagg aactgcctgt catgtttccg atg
//
LOCUS       DAOPFL010000149         2887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_48291_length_2887_cov_82.177260, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000149 DAOPFL010000000
VERSION     DAOPFL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48291_length_2887_cov_82.177260"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..2488
                     /locus_tag="PLG69_07315"
     CDS             248..2488
                     /locus_tag="PLG69_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPI43349.1"
                     /translation="MKRPFIILFVLFSAILFAGERGLTVKEIEVSGEVISISSPVQSL
                     IQVAPLRKVEKAEALKNWDTLQELNLNFRLPAKGQYGLQFIDLSQSVQPFGFPGCLSE
                     TAFQAVNKAPVWVQTELASILAELIPEKQTLWSNLILSAQDPYVDEICFCIANSSPQY
                     LNSAYALPELFTENAQLLYSIACELPYVEIMDTGTAISGGNYYSTTRYWKKNTSGEMQ
                     QITVPRELYYWYIVHPKLTDEIPAYINPAIIENNSTHNNNIALPPTGKFWRGYLYNLQ
                     EGDYPVLRDTLMQCQTLFNRDGTGNDAIRTIQWWINQQMSFTSNNERPHQPVRIITKH
                     IGRCGEYADLTSAVARLSLIPCTSILSYSLDHTWNEFWDENWVAWEPVNGYIDIPLVY
                     ENSWGTVFGSVFEIHPDGLLTPVTDKYSEGVSTIIIQVTDANQHPVDGARVILYIMQN
                     SMHFDCEQYTDNNGIATFLVGDNLEYFTRVETDFGSFPEIAGSYLTLTNNSVAGETYQ
                     YIITIPNVMPVPELEELPPPADPVQDYQFAVSYYSEGYYITGVTLWDDIYSLGVRPLH
                     YKYVATPGEVSFMVMDSDNILFWQIDNFGSGYSYSGPASEGTASFSIPLGSDWYAFMD
                     NSLHHRNAVKINGGLLFQHTGTAVQDETIPNVLLQYSIYPNPFNKTTCLSLKADKDFK
                     ADISILNLKGQVVKSWQNTFIKQGNSDFYWDGKDNKGRDVSSGVYFWKVQSRETAFTN
                     KILYLK"
BASE COUNT          880 a          540 c          586 g          881 t
ORIGIN      
        1 ctactgcccg aggtggtttt gctcccaatt ttaaaggaaa caatctgggc tttcgtatag
       61 tccgcaatgt tcattagcta ttgctgaaag taaggagtcg ttactgccga aaataattaa
      121 tgcactaccc tcaaataatt ttggcagtga aaactcatta tgcggaggaa cagcgtcctc
      181 cggttacaca gatcttggag aaatgctgtt ctctgtttac ccaaaacaaa gataaaggat
      241 atagggaatg aaaagaccgt ttattattct gtttgtgctt tttagcgcaa ttctttttgc
      301 tggtgagaga gggcttaccg ttaaagaaat tgaggtttcg ggagaagtga tttcaatttc
      361 ctctcctgtg caatcactaa ttcaagtagc acccttaaga aaggtggaaa aagctgaggc
      421 acttaaaaat tgggatacct tacaggaatt gaatttgaac tttcgtcttc cggcaaaagg
      481 acaatatggt ttgcagttta tagacctttc tcagagtgtt caaccctttg gtttcccggg
      541 atgtctttcg gaaacagcat ttcaggcagt taataaagct cctgtttggg tgcagacgga
      601 gttagcatcc attttagcag aactaatacc cgaaaaacaa acactttggt caaatctaat
      661 tctttccgcg caagaccctt atgtggatga aatatgtttt tgtattgcta attcttcgcc
      721 tcagtattta aattccgctt atgctttacc ggagcttttt acggaaaatg cgcaattgct
      781 ttattctatc gcttgtgaac taccttatgt ggaaattatg gatactggga ctgccatcag
      841 cggaggaaat tattattcca caacccgtta ttggaagaaa aatacttccg gtgaaatgca
      901 gcaaattacg gttcccagag aactttacta ctggtatatt gttcatccca agctgacaga
      961 tgaaattcct gcttatatca atcccgctat tatagaaaac aattcaacgc ataacaacaa
     1021 tatagctctt ccacctacgg gaaaattctg gcgtggatat ctgtataatc ttcaggaagg
     1081 tgattatccc gttctccgag atactttaat gcaatgtcag acacttttca atcgtgacgg
     1141 aacaggaaat gatgccatcc gcacaattca atggtggata aatcaacaaa tgagctttac
     1201 ttccaataat gaaagacctc atcagcccgt gagaattatt actaaacaca ttggacgctg
     1261 tggagaatat gccgatttaa cttcagccgt agcgcgttta tccttaattc cctgcaccag
     1321 tattttatcc tattctttag accatacctg gaatgaattt tgggacgaaa attgggttgc
     1381 ctgggaaccg gtaaatggtt acatagatat tcctctggtt tatgaaaaca gctggggaac
     1441 tgtctttggc agtgtttttg aaatccatcc cgatggactt ttaacacctg taacggataa
     1501 atattctgag ggggtttcta ccatcattat tcaggttaca gatgccaatc aacatcctgt
     1561 agacggagcc agagtaattt tatatattat gcaaaacagt atgcattttg attgtgagca
     1621 atataccgat aataatggaa tcgctacttt cctggttgga gacaacctgg aatattttac
     1681 gagggtggaa accgatttcg gttcatttcc tgaaatagcc ggaagttatc tgacattaac
     1741 taacaattct gtagcggggg aaacatatca atatattata acaattccca atgtaatgcc
     1801 ggttccggag ttagaagaat tgcctcctcc tgctgatcct gttcaggatt atcaatttgc
     1861 cgtatcttac tatagtgaag gatattacat tacaggggtt actttatggg atgatattta
     1921 ttctttgggg gtgcgacctc ttcattataa gtatgtagca actcccggag aggtatcctt
     1981 tatggtaatg gatagtgata atattctttt ttggcagata gataattttg gctccggcta
     2041 cagttattca ggtcctgcat ccgagggaac agcatctttc agtatccctc ttggctcgga
     2101 ttggtatgcc tttatggata attcattaca ccatcgtaat gctgtaaaaa tcaatggagg
     2161 actgcttttt caacataccg gaacggctgt tcaggatgaa acaataccta atgttttact
     2221 acaatattcc atctatccta atccttttaa caaaacaacc tgcttatccc tgaaggcaga
     2281 taaagatttt aaggcagata tttctatcct gaacctgaaa ggacaggtag ttaaatcctg
     2341 gcaaaataca ttcattaaac agggaaactc cgatttttac tgggatggaa aagataataa
     2401 gggccgggat gtaagctccg gagtttattt ctggaaagtc caaagcagag aaaccgcttt
     2461 taccaacaag atactttacc tgaaatagat aagttatttc cggaatgaga attcaattat
     2521 ccctatagaa aaaataaggt tgagataaag tcccaagggg cgacacaatc atatctcggt
     2581 tgtatgcgta aaagcaagtg gttgatgata attggggaag caggtgaaaa ttgtaagtca
     2641 aaaaatctct gccacgaaat aatgggtaaa ccacaaatat gttttatcat ttccattttg
     2701 aaaattcctt tgtccttccg gtgaaagcag gaaaccaact tatttgcata tagctaacta
     2761 aattttaagt atggaatttg gggatttttg taagggagca ttgctcactc attgcttacc
     2821 cattactaat gccattagca atgggtaagc aatgggtaag caatgctatt agggaagaga
     2881 atataat
//
LOCUS       DAOPFL010000150         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_49711_length_2825_cov_108.849458, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000150 DAOPFL010000000
VERSION     DAOPFL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49711_length_2825_cov_108.849458"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLG69_07320"
     CDS             complement(<1..430)
                     /locus_tag="PLG69_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HPI43350.1"
                     /translation="MAESKKTTDLNSEVKYLKGVGEHRAQLLSKLGIKTILDLMEHFP
                     KVYISRKLSATLGDLKPGDMLAFTAVISWVDVHQTAKGRNILSIGVSDGKVGIICSWF
                     TYPPVYEKMFLPGRLVWLNGTITEFNGQLEMVHPEFELIDD"
     gene            624..1667
                     /locus_tag="PLG69_07325"
     CDS             624..1667
                     /locus_tag="PLG69_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43351.1"
                     /translation="MKKMQKRLLVIVLFLLVFLLAEGQNFAQFKEELLNTKDWNTARQ
                     KIIAYIPTTNNVEELRELQSIWESVEPNVCKQYFFNAAQKNPHSPVYQYLALRLEEDE
                     TLQMKGAEELCLNYPDFYWGYRLYIVDFMAWLLNAELEIPDPLNGQELALKIIDEGYK
                     RFPDDDYFHIFQFHRYRLTQNYPQAEKELKLTKDRNLLMANWMRIKYFLVQEKNATLY
                     SSYLPPLFSDLIKSGQMASADSIYIFAEGYVEILQETASWQSLEQFFAQNPVLLNSAP
                     YFDVYAGLLARKEDWNALGNELLSAYNKKVIDSEHLSQYLTKWEDNLCHQPHWTELKQ
                     KAETQSQLPSPQY"
     gene            1675..2316
                     /locus_tag="PLG69_07330"
     CDS             1675..2316
                     /locus_tag="PLG69_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPI43352.1"
                     /translation="MKNPLRNFLTPDEQKILLFLGLIILGGCFLDYFGWNTLQATPTD
                     LDTLRQVVKEDKPLQLDIRIATFEELLCLSGIGEKRAKDIIAYRETNPFTSVNQIMNI
                     KGIGAKTYAKILPDLLVFGDTTNFKLSPTPSSTSKTKSATPSKANNTSPVNINTANLE
                     ELCTLSGIGEVKAQAIIDWREENGSFETIEDIMKVKGIGAKTFEKNKDRLTVK"
     gene            complement(2427..>2825)
                     /locus_tag="PLG69_07335"
     CDS             complement(2427..>2825)
                     /locus_tag="PLG69_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43353.1"
                     /translation="RNTGVFNYQNNRNPYIDHPEFVAKIWGGNDVEDAVLPVVPDLTI
                     NAVYPNPFTSELNISITTQKSALLTTSVYNIKGQLIYSESSVLIAGENKVFWKGQDNK
                     GQNTPAGIYLIKVQSADKQAVTKVLKVFEG"
BASE COUNT          910 a          569 c          499 g          847 t
ORIGIN      
        1 aatcatcaat cagttcaaat tccggatgca ccatttccag ctgaccgtta aattccgtta
       61 tcgtgccatt cagccaaact aaacgacccg gcaaaaacat cttttcataa acaggaggat
      121 aagtaaacca ggaacatatt atacctactt ttccatcact tacccctatg cttaaaatat
      181 tcctgccttt tgccgtttga tgaacatcta cccaagaaat tactgctgta aaagctaaca
      241 tatctcccgg cttcaaatcc cctaaagtgg cacttaactt gcgactgata tagaccttgg
      301 gaaaatgctc catcaaatcc aatatggtct ttatccccag cttgcttaaa agctgtgcac
      361 gatgttctcc aacccctttc aggtatttaa cttcgctatt caaatccgtg gtttttttac
      421 tttccgccat attggaagcc agcttagcaa acaacgcaaa attgtcaatt gcagaaaact
      481 gttacaacct aacactttaa cttttataaa aaacttatcc tgtaaccaac caaactgcta
      541 aactttgttt cttttgcttg acagatttta tgaggtaggc aataaagtaa aaaagctaaa
      601 ataggcattt aagaacggaa aatatgaaaa agatgcaaaa acgcttattg gtgattgttt
      661 tgttcctgct tgtttttttg ctggctgaag gacaaaactt tgctcaattt aaagaggaat
      721 tgcttaatac caaggattgg aatactgcca ggcaaaaaat tattgcctat attcctacta
      781 ccaacaatgt agaagaactt agagaactgc aaagtatttg ggaaagtgtg gaaccgaatg
      841 tttgtaagca atatttcttt aatgctgctc aaaaaaatcc tcattctcct gtttatcaat
      901 atcttgcttt acggctggaa gaagatgaaa cactccaaat gaaaggtgct gaagaacttt
      961 gcctaaatta tcctgatttt tattggggct accgtcttta cattgtggac tttatggctt
     1021 ggttactgaa tgcggaactg gaaattccag acccgctaaa cggtcaggaa cttgctttaa
     1081 aaatcattga tgagggctat aaacgctttc ctgatgatga ttattttcat attttccagt
     1141 ttcatcgcta tcgcttaaca caaaattatc ctcaggcaga aaaggaactg aaactgacta
     1201 aagaccgcaa tttattgatg gctaactgga tgcggataaa atatttcctg gtgcaggaga
     1261 aaaatgccac tctgtatagt tcatatctgc cccctctttt ttcggacttg attaaaagcg
     1321 gtcaaatggc ttcagctgat agtatttata tttttgctga gggctatgtg gaaattttgc
     1381 aggaaacagc aagttggcaa agtttagagc aattttttgc tcaaaatcct gttttgctaa
     1441 attcagctcc ctattttgat gtttacgccg gtcttttagc ccgcaaagag gactggaatg
     1501 ctcttggcaa tgagcttctt tctgcttata acaagaaagt gatagatagt gaacacttaa
     1561 gccaatatct tacaaaatgg gaagataacc tttgccatca accccattgg acagaactga
     1621 aacaaaaagc agaaacccaa tctcagcttc cttctcctca atactaatat gaaaatgaaa
     1681 aatcccttgc gcaattttct cactcctgat gagcaaaaga tattgctttt tttagggcta
     1741 ataattctgg gtggttgttt tctggactat tttggctgga atactttgca ggcaacccct
     1801 acagatttag ataccttaag gcaggtggtt aaagaagata aacccttgca actggatatt
     1861 cgcattgcca cttttgaaga attgctctgc ctttccggaa ttggagaaaa aagagcaaaa
     1921 gatattattg cctatagaga aacaaatccc ttcacctcgg taaaccagat tatgaatatt
     1981 aaaggcatcg gagccaagac ctacgctaaa attttgcccg atttactggt ctttggagat
     2041 actacaaatt tcaagctttc tccaacccct tcttccactt ctaaaactaa atccgcaacc
     2101 ccttccaaag caaataatac ttccccagtc aatattaata cagctaatct ggaagaacta
     2161 tgcacccttt ctggaattgg agaagtgaaa gcccaggcaa ttattgactg gcgtgaggaa
     2221 aatggcagtt ttgaaaccat tgaagatata atgaaagtga agggaattgg agccaagacc
     2281 tttgagaaaa acaaagaccg cttaactgtg aagtaaagaa ataaagaatg tgtgaatgtg
     2341 aggtatcctg aaaatagcgg actaaaatta aggaaaaaat ccggttaagc caattgaacc
     2401 agttatctgt gttctatttt aaaatctcaa ccctcaaaca ctttcaagac ctttgtaact
     2461 gcttgtttat ctgcactctg cacttttatc aaataaattc cggcaggagt attttgtccc
     2521 ttattatcct gtcctttcca aaagacctta ttttctccgg caattaatac actgctttcg
     2581 ctgtaaatca attgaccctt aatattatag acagaagttg tcagcaaagc tgatttttga
     2641 gtagtgatac taatattcag ttcagaagta aaaggattgg gataaactgc attgattgtc
     2701 aaatcgggaa cgacaggcaa aacagcatct tcaacatcat tcccgcccca gattttagca
     2761 acaaattccg gatggtcaat ataaggatta cggttatttt gataattaaa aacccctgta
     2821 ttgcg
//
LOCUS       DAOPFL010000151         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_49986_length_2815_cov_82.776812, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000151 DAOPFL010000000
VERSION     DAOPFL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49986_length_2815_cov_82.776812"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..895)
                     /locus_tag="PLG69_07340"
     CDS             complement(110..895)
                     /locus_tag="PLG69_07340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPI43354.1"
                     /translation="MSYSKYFSAEGFCLYWGNCIEILEELEPNSIDMIFADPPYFLSD
                     GTITCKSGKMVSVKKGDWDLLDEFNNKSEFHHKWISACRQILKPSGTIWISGTYHSIY
                     QCGYELQKQGFRIINDICWFKPNAAPNLTRKCFTASHETILWAIKDPLQKQKYHYELM
                     KNTDWMGDIINKKGKQMRSVWCIPTTPAREKIHGKHPTQKPIALLERIILSSTDEGDL
                     VLDPFNGSGTTGVVAKKYHRNYIGIDINIDYLKLTIERIKDTL"
     gene            complement(898..1797)
                     /locus_tag="PLG69_07345"
     CDS             complement(898..1797)
                     /locus_tag="PLG69_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HPI43355.1"
                     /translation="MKQLPVYKKLKLNSPEKVFNYLINTMLPTINNWSYLVDWEKVSK
                     HIKDIEICLNTMNYLIGKKNIHNEFKYLLRKHPEIKHILPVLVACRKDSITLLTSNDE
                     NDLQAKEFNFKNLSDEDALNFADRAGLLQLLSSNKIKNLVDYVFGIEVGLDSNARKNR
                     AGKAMEQLMNKHIKKICEKNNLEYLASANKSKVKKQWGIDLPVDKTNRKIDFIIKKDR
                     QLFLCEVNFYSVGGSKLKSTAGEYKNIYNFWKSHNLQFIWITDGWGWKTTQRPLQEAF
                     NELDYILNIKMVFDGVLEYILTN"
     gene            complement(1886..>2815)
                     /locus_tag="PLG69_07350"
     CDS             complement(1886..>2815)
                     /locus_tag="PLG69_07350"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HPI43356.1"
                     /translation="TTQMKLFNFDAKPFLKWAGGKGQLIPELVDRLPDKFKKSMHIPV
                     YIEPFIGGGAFFFYLQSCFQIDKSIIIDINRDLIITYQVIKYAVENLIALLSELDNKY
                     NNASQTERTTLYYDIRKRFNYSKKHLDTTYNELWIRHSAYMIFLNRTCFNGLYRENKN
                     GEFNVPHGRYDKPSICQAENLWDANKALSNTKILQGDFSLAANYVEENTLIYYDPPYR
                     PLTKTSNFNSYAKEGFADKDQIRLANFFQEMDRSDVYQILSNSDPRVTNPADDFFDNL
                     YKEYKISRIQAKRMINSDAEKRGNITEIIVKNY"
BASE COUNT          873 a          476 c          431 g         1035 t
ORIGIN      
        1 cgctcacgag tcctcactac aaaacaaatt tgagggtagc gtaaaagcta tttcggcagt
       61 gacgactctc aacttgaatt gtaaatataa cttcaaagtt tccacaaggt taaagagtat
      121 cttttatcct ttcaatggtc aatttcaaat aatcaatatt gatatctatt cctatataat
      181 ttctatgata ttttttagca acaacaccgg tagtaccgct tccattaaag ggatcgagaa
      241 caaggtctcc ttcatcggta gaagataaaa tgattctttc cagtaatgct atgggctttt
      301 gagttggatg ttttccgtgt attttttccc ttgcaggtgt agttggaata caccaaacac
      361 ttctcatttg tttacctttc ttattgataa tatctcccat ccagtcagta tttttcatta
      421 gctcataatg atatttttgt ttctgtaagg ggtctttaat tgcccaaaga attgtttcgt
      481 gagaagcagt aaaacatttt ctggtaaggt ttggagctgc attaggtttg aaccagcaaa
      541 tatcattaat aattctaaac ccttgttttt gtaactcata tccacattga taaattgaat
      601 ggtaggttcc gctaatccaa atagttcctg atggttttaa tatttgtcta catgcagaaa
      661 tccatttatg gtggaattct gatttgttat taaattcatc aagcaaatcc cagtcaccct
      721 ttttcacact taccattttt ccgcttttac aagttatggt tccatccgat aaaaagtagg
      781 gaggatcggc aaaaatcata tctattgagt tgggttcaag ttcttctaaa atttcaatac
      841 aatttcccca atataagcag aaaccttcag cagaaaaata tttactatag ctcataatca
      901 attagtaagg atatattcta aaacaccatc aaaaaccatt tttatattca ggatataatc
      961 aagctcatta aatgcttctt gcaatggtct ttgcgttgtt ttccaacccc aaccatcagt
     1021 tatccatata aattgtaggt tatgactttt ccaaaagtta tagatatttt tatattcacc
     1081 tgctgttgat tttaattttg aaccgccaac gctgtaaaaa ttgacttcac ataaaaataa
     1141 ctgacggtct ttttttataa tgaagtcaat tttacgattt gttttatcta caggaaggtc
     1201 aattccccat tgttttttta cttttgactt gttagcagat gctaaatatt caaggttgtt
     1261 tttttcacaa atctttttaa tatgcttatt cattaattgt tccattgctt ttcctgctct
     1321 atttttgcgg gcattggaat ccaatcctac ttcaattccg aaaacataat caaccaagtt
     1381 cttaattttg tttgaactta ataactgtaa taagccagct ctgtcagcaa aatttaaggc
     1441 atcttcatca gaaaggtttt taaaattgaa ctcttttgct tggaggtcgt tttcatcatt
     1501 agaagttaac agggtaatac tatcctttct gcaagcaaca agaacaggta aaatatgctt
     1561 aatttccgga tgttttcgca gaagatattt aaattcattg tggatattct ttttgcctat
     1621 gagatagttc atcgtattta ggcatatttc aatatccttt atgtgcttgg atactttttc
     1681 ccagtcaaca agataagacc agttattgat tgtaggtaac atagtattta ttagatagtt
     1741 aaatactttt tcagggctgt tcagttttaa ttttttatat acgggtaatt gtttcaaggt
     1801 aaacctccca atctatgata aacattatat atactatata aagcataatt tttattagtt
     1861 tagaactgag acatactcca tcacctcaat aattcttaac aattatttca gtaatattac
     1921 ctcttttttc ggcatcggaa tttatcattc tttttgcttg tattcggctt atcttatact
     1981 ctttatagag gttatcaaaa aagtcatccg cgggatttgt aactctggga tcggagtttg
     2041 ataaaatctg atatacatca gatctatcca tttcctgaaa aaagttagct aatctgattt
     2101 ggtctttatc ggcaaaacct tctttggcat aagaattgaa attggaggtc ttggttaatg
     2161 gtctataggg aggatcataa taaattaagg tgttttcttc aacataattt gcagccaagg
     2221 aaaagtcacc ctgtagaatt tttgtattag acagtgcttt attcgcatcc caaaggtttt
     2281 ctgcttgaca tattgagggt ttatcataac gaccatgagg gacattaaat tccccgtttt
     2341 tattctctct atataaaccg ttaaaacagg tcctattaag aaaaatcata taggcagagt
     2401 gtcttatcca taattcgttg taagtggtat caaggtgctt tttactataa ttaaaccttt
     2461 ttctgatatc ataatacaaa gtagttctct cagtttggga agcattatta tatttattat
     2521 ccaattcgga taataaagca attaaattct ctacagcata tttaataact tgataggtta
     2581 tgatcaaatc acggttgata tctattatga tagatttatc tatctgaaaa caggattgta
     2641 aataaaagaa aaatgcacca cctccaataa atggttcaat ataaacagga atatgcatac
     2701 tttttttgaa tttatccgga agccgatcaa caagttcggg aattagttgt cctttaccac
     2761 ctgcccattt taaaaagggt tttgcatcaa agttaaacaa tttcatctga gtggt
//
LOCUS       DAOPFL010000152         2751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_51572_length_2751_cov_84.153190, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000152 DAOPFL010000000
VERSION     DAOPFL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51572_length_2751_cov_84.153190"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="PLG69_07355"
     CDS             complement(<1..1839)
                     /locus_tag="PLG69_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43357.1"
                     /translation="MKKLIIGLVILLSCQFALAIITDNIYYVNARQYFELGNYASAKQ
                     NLDMIQAPDNQDPEYALLRGKVHLALGDYKQAHIWLTEYQNKSLGTDPLVQKDLLQML
                     YEVALYQEQTSVSVSLGKLKGSINTSDSEYAPVFTPDNKYMYFSSLRRSDFGKENIFL
                     SQQHNNVWSEAIEVTELCTDYNESFGSLSLDGNTAYLFGYYSKDTTNGDIYQTTLDSK
                     GRWTKPIMIKEVSSKYYDLQPFVWRDKVMFLTSNRDGDHKNYDIYVSENINGIWTTPV
                     SVGNIVNTPYDEQSPFLSPDGKYLYFASFGHNSYGGNDIFVSTRIGNSWTEWSKPVNM
                     GPIINSVKDDRYFVIAPDGRTAYLCSNRAGGMGQEDIYTIDLGLLDKIKEQIVLLTGV
                     KPTEEKPVVPQAFKELKISGVVVSDKNQPVKTDIIWVYDLGDKTFMRIISSDELGTFS
                     FTLPGDTKDIAFEINTPGYKKTTGKIEIPPDKNEIFVNITLPVETGIGEGGNLAINGK
                     VLDEENNPVPCSIRWSYVFEGELTEVIVESNNEGVFKLYTPIMEKLKYRIEEPGYAVR
                     EEIITIPKNVNSYDTIIRLVRLGKEVTLNGKVFDDNDNPLVAMVAWI"
     gene            complement(1943..>2751)
                     /locus_tag="PLG69_07360"
     CDS             complement(1943..>2751)
                     /locus_tag="PLG69_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43358.1"
                     /translation="DNVSSAYLNPAVLADVKTIEFTSGTRQNMAWDRNHNAVALGFVL
                     PIGYVAASWQNATVSDIPGYDVNGNPTGDFSNSDNTIALSFAAKISKLNLGITPKFYL
                     SNQDNDSDTGYGLDLGFLYHINRYFNVGMVARDLIASYPGEEDDIPIEFIPGVAAYPI
                     PGLIIAADLSGRDNFKDSKLRLGVEYWLGVRDDAEIGSSLSGIRIRESATWSDIISKV
                     QAGIRAGVNDGAFTAGAGLRFKMLELNYAFQMAHDEYLNDTHSVSLLLRF"
BASE COUNT          856 a          602 c          462 g          831 t
ORIGIN      
        1 aatccaagca accatagcta ctaaaggatt atcattatcg tcaaagacct taccgttaag
       61 tgtaacttcc ttccccaatc taaccaaacg aataattgta tcataactat tcacattctt
      121 aggaatagtt ataatttctt cccgcactgc atagccgggc tcttcaattc tatatttaag
      181 cttttccatt atcggtgtat agagtttaaa aacaccttca ttattgcttt ctacaataac
      241 ttcggttaat tcgccctcaa aaacatagct ccaacgaata ctgcagggaa caggattgtt
      301 ttcttcatcc agaactttac cgttgatagc aagatttcca ccttcaccaa ttcctgtttc
      361 tacgggaaga gtaatattta caaatatctc atttttatcg ggtggaattt caattttacc
      421 tgttgttttc ttatatccgg gagtattgat ctcaaaagca atatctttgg tgtccccggg
      481 aagggtaaaa gaaaaagttc ctaattcatc tgagctaata atacgcataa atgttttatc
      541 accaagatca taaacccata taatatctgt tttcacaggt tgatttttat cactcacaac
      601 cacaccgctg attttcaatt ctttaaatgc ctgaggaaca acgggtttct cctcggttgg
      661 cttaactccc gtaagtaaaa cgatctgttc ctttatctta tccagtaaac caagatctat
      721 tgtataaata tcttcctgcc ccataccacc ggcacgattg gaacataaat atgcggttct
      781 gccatcagga gcaataacaa aataacggtc atcctttacg gaattaatta tcggacccat
      841 atttaccggt ttgctccatt ccgtccagga attacctatt cttgtggaaa caaagatatc
      901 attaccacca taactgttat gtccgaaaga agcaaaataa agatacttac catccggact
      961 taaaaaaggc gattgttcgt cataaggtgt attaacaata ttacctacac taacaggagt
     1021 tgtccagatt ccgtttatat tttcggaaac ataaatatca tagtttttat gatctccgtc
     1081 ccgattactg gttaaaaaca taactttatc acgccagaca aagggctgca gatcataata
     1141 tttactggag acctccttaa tcattattgg tttggtccag cgtcccttac tatctaaagt
     1201 cgtctgataa atatctccat tagttgtatc tttagaatag tatccaaata aataagcagt
     1261 attgccatcc agagaaaggg aaccaaacga ttcgttataa tcggtgcaaa gttcagtaac
     1321 ttcaattgct tcactccaaa cattattgtg ctgctgagaa agaaaaatat tctccttccc
     1381 gaaatcactg cgtcggagtg aactgaaata catatatttg ttgtctggag taaataccgg
     1441 agcatattct gaatcactgg tattgatgct acctttcagt ttccccaaag ataccgaaac
     1501 agatgtttgt tcttgataca gagcaacctc atatagcatc tgaagcaaat ctttctgcac
     1561 taagggatct gtacccagtg atttattttg gtattccgta agccaaatat gcgcttgttt
     1621 gtaatctccc aaagccagat gaactttccc gcgcaacaga gcatattcag gatcctgatt
     1681 atccggtgct tgaatcatat ccagattttg tttggcggaa gcataattac ccaattcaaa
     1741 atattgccgg gcatttacat aatagatgtt atcagttatt attgcgagag caaactggca
     1801 agacaggagg attaccaacc cgattattag tttcttcatt atatctcctc taatgtgagg
     1861 gcttcccctg agaaaaaatt ctcaggggaa aaccacacac aacttttttt attgcaaacc
     1921 agatatcctg gttacaagcg ttttagaaac gcagtaacag ggatactgaa tgagtatcat
     1981 tcaagtattc gtcatgcgcc atctggaatg cataattcag ttctaacatc ttaaaccgca
     2041 gtccggcacc agctgtaaaa gcaccatcat taacaccggc acgaattcca gcttgaactt
     2101 tggatatgat atcagaccag gttgcactct ctctaatacg aattccggat aaactggagc
     2161 caatttcagc atcgtctcta acgccgagcc aatattcaac tcccaaacgc aattttgagt
     2221 ccttgaaatt atcgcgacca gataggtctg ctgcaataat caaacccggt ataggataag
     2281 ctgcaactcc aggaatgaac tctataggaa tatcatcttc ttctcccgga taactggcaa
     2341 ttaaatccct ggctaccata cctacattaa aatagcgatt gatatgatag agaaatccaa
     2401 ggtctaagcc ataaccggta tcggaatcat tatcttgatt ggagagatag aactttggtg
     2461 tgatgcctaa attcagcttg gagattttgg cagcaaagct caaagctatt gtattatcac
     2521 tattgctaaa atcccctgta gggttgccat taacatcgta tcctggaata tcactaacag
     2581 tagcattttg ccaggatgca gcaacataac ctatgggcag aacaaaccct aaagcaactg
     2641 cattatgatt tctatcccaa gccatattct gtcttgtgcc tgaagtaaat tctatcgttt
     2701 taacatctgc caaaactgcc ggattcaggt atgcgcttga gacattatcc a
//
LOCUS       DAOPFL010000153         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_51649_length_2748_cov_112.639064, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000153 DAOPFL010000000
VERSION     DAOPFL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51649_length_2748_cov_112.639064"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLG69_07365"
     CDS             <1..555
                     /locus_tag="PLG69_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPI43359.1"
                     /translation="MDNLKNVLMALQILQSNAGFSFSPRRITVSTCGVVPGIIALADS
                     GIKAKLALSLISAVEAKRKQLMPVNNQYNLIQLKQALLYYLRKTSFRITIEYILIPNF
                     NMDSEDLAALRKFTGDLSCKINFIPYNPGRNSPFRAPTETEIKDFMQRAQKLPQAITL
                     RKSRGADIFGACGQLTKNNLGEKK"
     gene            552..1328
                     /gene="deoC"
                     /locus_tag="PLG69_07370"
     CDS             552..1328
                     /gene="deoC"
                     /locus_tag="PLG69_07370"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPI43360.1"
                     /translation="MKDIPQIAKELFVDNHPAQCNGGHIICLNCYHCHLKFPDQIYDP
                     NQGLASVIDATILKAETKKEEIAKLCKTANQYKTASVCVNSHFIPLAKGLLEKPVKSC
                     TVINFPLGASCPQAIVNEAKAVLEIGVEEVDLVQNLSAFLSGDYDNDLQCMQEVVKLC
                     KKHKVLLKVILETCYLTEEQIIISCLLAKKAGADFVKTSTGFGTAGATKENVALMRKV
                     VGPKIGVKAAGGIRTKEQALAMLSAGANRIGASSVKTILQ"
     gene            1341..>2748
                     /gene="thyX"
                     /locus_tag="PLG69_07375"
     CDS             1341..>2748
                     /gene="thyX"
                     /locus_tag="PLG69_07375"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPI43361.1"
                     /translation="MKVNIAGFNIDKELIDKLNSPLATPELISASYARISRSNKSIEE
                     LRKEAMKELEKARKTNTNIIFAMGHSSVAEHSVFNIDLIGISRALTELIQRSRIASFT
                     EKSQRYVTFSKDYVVPAEFANRPKLKQPYKKLMDELFAEYELSFNALNELYKKQKPEL
                     KTRERECLAKEDARYILPLSTKTQMGMTINARSLENLLRRLSKSPLKEAAELKELLYT
                     KVSAIAPSLIRYIDNDDYTGKVDLEKVGFTGFLQQELPWVKELDLENKVRVINLPTNA
                     DDTILASIIYQQGEFNWNETKETVAHLPRIIKQQLWNQVFKDLKPWHKVPRAFETVDF
                     SFELSMSESCWAQFKRHRYCTALRKAGSSLTAKIPPAIKAVNRENIWKELNNKIIRMG
                     SALPPLLAYLNPYFRTNASIVTVFVKMNLREIYHFVRLRSDENAQWEIRELSQLMAAQ
                     VKKNAPQAASFLCGKSEMN"
BASE COUNT          927 a          503 c          555 g          763 t
ORIGIN      
        1 atggataacc tgaaaaatgt tcttatggca ctccaaattt tgcaaagcaa tgcagggttt
       61 tctttcagtc caagacgcat aacggtttct acctgtggag ttgttcccgg tattatagcc
      121 cttgcagata gcggaattaa agccaaactt gccctttctt taatttctgc tgttgaggca
      181 aaacgcaagc aattaatgcc tgtaaacaat caatataacc ttattcagct gaaacaggca
      241 ttattatatt acctaagaaa aacttccttt cgcataacta ttgaatatat cctcattccg
      301 aactttaata tggatagtga agaccttgcc gctttaagaa aattcaccgg cgacctatcc
      361 tgtaaaataa attttatccc gtacaatcca ggcaggaatt ccccttttag agctcctaca
      421 gaaacagaaa ttaaggactt tatgcagaga gcgcaaaaat tgccccaggc aataacttta
      481 cgcaaaagca gaggagcaga tatttttggt gcctgcggac aacttacaaa aaataatctg
      541 ggagaaaaga aatgaaagat attcctcaaa ttgccaaaga actttttgtc gacaatcatc
      601 ccgctcaatg taacggtgga catatcattt gtctgaactg ttatcattgt catttaaaat
      661 tccccgatca aatttatgat cccaatcagg gtttggcttc cgtgatagat gctacaattc
      721 taaaggcaga aaccaaaaaa gaagagattg caaaactctg taaaacggca aatcaatata
      781 aaactgcctc cgtttgcgtt aattcacatt ttatcccttt agcgaaagga cttctggaaa
      841 agccggttaa aagttgcacc gttatcaatt ttcccttggg agcatcttgt ccccaagcaa
      901 ttgttaatga agcaaaagcg gttttggaaa tcggagtgga agaagtggat ttggttcaga
      961 acttaagtgc ttttttaagt ggtgattacg ataacgattt gcaatgtatg caagaagtgg
     1021 ttaaattatg taagaagcat aaggtgttgc tgaaagttat tttggagacc tgctatctta
     1081 cagaagaaca aataattatc agttgcttgc tagcaaaaaa agcaggagcg gattttgtaa
     1141 aaacctccac cggttttgga actgcaggtg ctactaagga aaatgtcgca ttaatgcgta
     1201 aagttgtagg acctaaaatc ggagtgaaag cagcaggtgg tattagaaca aaagaacagg
     1261 cactggcaat gctttctgca ggtgccaaca gaattggagc ttccagtgtg aagacaattt
     1321 tacagtaggg attacacaca atgaaagtaa atattgccgg attcaatata gataaagaac
     1381 ttattgataa actgaatagt cctttggcta ctcctgaatt gatttccgcc tcttatgcac
     1441 gtatcagcag aagcaataaa agcatagaag aattaagaaa agaggcaatg aaagaactgg
     1501 aaaaggcaag aaaaaccaat accaatatta tctttgcaat gggtcatagc tctgtggcag
     1561 aacattcggt attcaatatt gatctaatcg gaatttccag ggcgttaaca gaattgattc
     1621 agcgcagtag aattgcttcc ttcacggaaa aatctcaacg ctatgtaact tttagcaagg
     1681 attatgttgt ccctgcagaa tttgcgaatc gtcccaaact gaaacagccc tacaagaaac
     1741 tgatggacga actgtttgcc gaatatgaat taagttttaa cgccttaaat gagctgtata
     1801 aaaaacagaa accggaactt aaaaccaggg aaagggaatg ccttgccaaa gaagatgcgc
     1861 gttacatttt gcctctttcc acaaaaaccc aaatgggaat gacaattaat gcccgttctc
     1921 tggaaaatct cttgaggcgt ttaagtaaaa gtcccttaaa agaagcggct gaactgaagg
     1981 aattattata tacaaaggtt tcggcaattg ccccatcttt aattcgttat atagataatg
     2041 atgattacac aggtaaagtg gacttggaaa aggttggttt tactggtttt ctgcagcagg
     2101 aattaccttg ggtgaaagaa ctggatttgg aaaataaagt gagggttatt aaccttccta
     2161 ctaatgctga tgacactatt ttagcaagta ttatctacca gcagggagaa tttaactgga
     2221 atgaaacaaa ggaaacggtt gcccatttgc ccagaataat caaacagcaa ctctggaatc
     2281 aggtttttaa agacctgaaa ccctggcata aagttccccg tgcctttgag acagttgatt
     2341 tcagttttga actttctatg agcgaaagtt gctgggctca atttaaacga catcgctatt
     2401 gtactgcttt gcgtaaagcg ggaagttctt taacagcaaa aattcctcct gccatcaaag
     2461 ctgttaacag ggaaaatata tggaaagaac tgaataataa aattattaga atgggcagtg
     2521 cacttccccc cctattggca tatctaaacc cttattttag aactaatgct tctattgtta
     2581 ccgtttttgt gaaaatgaac ttacgggaaa tatatcattt tgtgcggttg aggagtgacg
     2641 aaaatgccca atgggaaatt agagagctgt cacaattaat ggctgcacaa gttaaaaaaa
     2701 atgctccgca agccgcttca ttcctttgtg gaaaaagcga aatgaata
//
LOCUS       DAOPFL010000154         2744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_51763_length_2744_cov_110.031982, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000154 DAOPFL010000000
VERSION     DAOPFL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51763_length_2744_cov_110.031982"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1397
                     /locus_tag="PLG69_07380"
     CDS             126..1397
                     /locus_tag="PLG69_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPI43362.1"
                     /translation="MKKRLFPVLLCCVLIFSACSQNARLSKKTQKNLNKGNYEEAIQD
                     IIKTLKKNPNNATAQDLLVQSWQSYRSAEQKKIERIIQSDEINKWEQVYQEYSALQKT
                     GEEIQSLPPLINPYSGYKVTIEIPDYTEQIKQSKENAAEAHYQAGIRYAKISNDRYTQ
                     KKAALEFKAALALIPNYQDADLRYEQCRKLAIKRIAVSPFTDKSNTSGKYGAVSDILT
                     DFMVSRLISAAVNNEFVEIISRSQLETVMKEQQLSASGLVNDASSVRLGQILGANEIL
                     AGSILQISVSPERTVSVQSEDETEVVLRTEEYTDDEGNTQEREIKGKVYFRYRKFTKT
                     ASVNISTSYSILDVETGKILLQETVEVKNPWSDTWARKISGDDRALSSSTKKLLEKPE
                     PFPPSVNEMVLETLKNTGNEIVNKVSGYLLQ"
     gene            1442..2731
                     /locus_tag="PLG69_07385"
     CDS             1442..2731
                     /locus_tag="PLG69_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPI43363.1"
                     /translation="MAKRQPEWIVERPYNNEYYIAIVKVPRKAPNYVELARNNAILEI
                     STQISVQIDSDIALKETEENGIPSSEIISRIRSSSKNILKDLQLVGTYETKNDYWAYY
                     RLSKSEYSAWRKAQCNQAMAQALNLLADFDSSASNIASGIASLLHGLELIVDYTDMDL
                     TTLYKGNEINLYNELFYRLNRLPENLSFKFATNEIDIVAKQRERKTVSLSVSCNKNGQ
                     EYPCSNFPLCFNFSSGTGEIIPQAITDENGKAELIINRITSFSNPQFIEAKPNKDFWL
                     VGRENTVVKTMFNNLQFMPAVLKLNVRRPKAYLEYSFDNTPGTDFRNILVKKLQDLDL
                     EVTENQNTSDYTFKVDIYNRSTNYLPLLNQYSATADAYIELLQTGNGKSIYNTNITGI
                     KSTASLPEIARKMSELNAVNEICDKVMFMLVEQYIMF"
BASE COUNT          924 a          561 c          512 g          747 t
ORIGIN      
        1 atccagtaaa ttcagttatc cagtcatctg tgttctatca tcctcagtta aaatcagctt
       61 gacacaaacc cttgtcaatc cataaatgtt ttcaataaag ttattcatcc ctatcaggag
      121 ttttaatgaa aaaacggtta tttccggttc ttctttgttg cgtgctgatt ttcagtgcct
      181 gctctcaaaa cgcccgcctt tccaaaaaga cccaaaagaa cttaaataaa ggtaattacg
      241 aagaagcaat tcaggacatc ataaaaaccc ttaagaaaaa tccgaataat gcaacagctc
      301 aagacctttt ggtgcaatcc tggcaaagtt accgttcggc agaacagaaa aagatagaaa
      361 gaatcatcca aagcgatgaa ataaataaat gggaacaggt ctatcaggaa tattccgctc
      421 tgcaaaaaac cggagaagaa attcaatccc tacctccttt gataaacccc tattccggct
      481 acaaagtaac catagaaatt cccgattaca cggaacaaat aaaacaaagc aaagaaaatg
      541 ctgctgaagc gcattatcag gcaggaattc gttatgccaa aatcagtaat gatagatata
      601 cacaaaaaaa agcagctttg gaatttaagg cagcgcttgc tttgattccc aattatcaag
      661 atgccgattt aagatatgaa caatgccgca aactggcaat taaaagaatt gccgtttctc
      721 cctttactga taagagcaat acttccggaa aatacggtgc cgttagtgat attttaaccg
      781 actttatggt ctcccgcctt atttcagctg cagtaaataa tgaatttgta gaaattatat
      841 cccgttccca attggaaacg gtgatgaaag aacaacagct ttccgcttca ggtttggtga
      901 atgatgccag ttccgttcgt ttaggtcaaa ttttgggtgc taatgaaatc ctcgctggca
      961 gcattttaca gattagcgtt tccccggaac gcactgttag tgttcaaagt gaagatgaaa
     1021 cggaagttgt tcttcgcact gaagaatata ccgatgacga aggaaacact caggaacgcg
     1081 aaattaaagg aaaggtttat ttccgttacc gcaagtttac caaaacagca agtgttaata
     1141 tttccacctc ctacagtatt ctggatgtgg aaacgggaaa aatactactg caagagacag
     1201 tggaagttaa aaatccctgg agcgatactt gggcaagaaa aatttctgga gatgaccgtg
     1261 ccttaagttc cagcaccaaa aaattgctgg aaaagccgga accctttcct ccttcagtaa
     1321 acgaaatggt gctggaaacg ctgaaaaata caggaaacga gatagttaat aaagttagcg
     1381 gatatttatt acagtgaagc gttttttata cctcctgctc ttaatcttta ccgcatgcag
     1441 tatggcaaaa cggcaaccgg aatggattgt ggaaagaccc tataataatg aatactatat
     1501 cgctattgta aaagttcccc gtaaagcacc taattatgta gaacttgccc gaaataacgc
     1561 tattctggaa atatccactc aaataagcgt gcagattgat tccgatattg ccttaaaaga
     1621 aacggaagaa aatggcatcc cctcctctga aataatcagc agaatacgca gttccagtaa
     1681 aaatatactc aaagacctgc aactggttgg cacttatgaa actaaaaatg actattgggc
     1741 ttactatcgc ctttccaaaa gcgaatattc tgcctggcga aaagctcaat gtaatcaggc
     1801 aatggctcaa gcattgaatt tacttgccga ttttgattcc tccgcctcta atatcgcttc
     1861 cggcatagct tctcttttgc atggactgga actgattgtg gattataccg atatggattt
     1921 gacaactctt tacaaaggca atgagattaa cctctataac gaactattct atcgtctgaa
     1981 ccgtttaccg gaaaacctta gctttaaatt tgccaccaac gagatagaca ttgtagccaa
     2041 gcaaagagaa cgcaaaacgg tttctctttc tgtcagttgc aataaaaacg gacaagaata
     2101 tccctgctct aactttcctt tatgcttcaa tttcagcagt ggcacgggag aaatcattcc
     2161 ccaagccata acagatgaaa acggtaaagc ggaattaatt atcaatcgca taacctcttt
     2221 cagcaatccc caattcattg aagccaaacc gaataaggat ttttggctgg taggaaggga
     2281 aaataccgta gttaaaacaa tgtttaataa tctgcaattt atgcctgctg ttttgaaatt
     2341 aaatgtccgc cgtcccaaag cttaccttga atatagtttt gataacactc cgggaactga
     2401 cttccgcaat attttagtaa agaaactgca ggatttggat ttggaagtta ccgaaaacca
     2461 aaacaccagc gattatactt tcaaagttga tatctataac cgtagcacta attatcttcc
     2521 acttctcaat caatattctg ctacagctga tgcttatatt gaactcctgc aaaccggtaa
     2581 tggcaaaagt atttataaca ccaacataac cggaattaaa agcactgctt ccttacccga
     2641 aatagccagg aaaatgagtg aactgaatgc tgtgaatgag atttgtgata aagtgatgtt
     2701 tatgctggtg gaacaatata ttatgtttta gagtgctggg gtgc
//
LOCUS       DAOPFL010000155         2740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_51863_length_2740_cov_61.239851, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000155 DAOPFL010000000
VERSION     DAOPFL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51863_length_2740_cov_61.239851"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLG69_07390"
     CDS             complement(<1..596)
                     /locus_tag="PLG69_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPI43364.1"
                     /translation="MKNPDFLQINPDFSAPEKPKPKLNFSWKTNEQIEVKPLYTKDDI
                     ISTEHLDYLSGLPPFLRGPYPTMYIQRPWTIRQYAGFSTAEESNAFYRRNLAMGQKGL
                     SIAFDLPTHRGYDSDHPRVIGDVGKAGVAVDSILDMKILFDQIPLDQMSVSMTMNGAV
                     IPIMAFYIVAAEEQGVPPQALNGTIQNDILKEYMVRNT"
     gene            complement(593..2494)
                     /locus_tag="PLG69_07395"
     CDS             complement(593..2494)
                     /locus_tag="PLG69_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPI43365.1"
                     /translation="MDSEIKLNLLQEFPPPSFEEWLQVVQAGLKGADFDKVLKTKTTE
                     GITLQPIYRKEDIEGIPFLGSQPGSSPFVRGNNPLRFLTEGWLIAQNHTDTDPKILNQ
                     KLLKELNQGLTAVNITLSNKISTEGVKIQNLADWETLLEGINLQAAPLFMQLGIEDTE
                     ILITFVQYAVKHNIELKSLESAIGLDYIGELAEKGYLPCTLADAEQKLAEIVKWNVEK
                     MPRNRIISIDTSVYEEAGASSVQELAFALATAIYYLRQLLNSGWTIEQVAPLLQVKLA
                     LGSNFFMEIAKVRALRLLWAEMIKSFGGEEDKQKVWIHGKTAKFNKTIYDIYVNVLRT
                     TTESFAGVIGGVDSLEIGCFNELIAQPDEFSARLARNQQIILKEEAHFDKVIDPAGGC
                     YYIEWLTNEMAKSAWQLMQEIEGKGGMLEYVTKGEIHQEVETIVRERIEAVNKRKNIF
                     VGINMYANPEEKLPTLQRDDKPVSKEDKAFILKDGALPKHRAVEEMENLRMQMEASQT
                     NKKIFLLNLGTLADYKARADFALNFFPVGGLEVIYPNGFNTVEEAVTAAEKSGASAFC
                     ICSTDENYVSLVPEICAKMKGKILILAGYPADKIEEYKQAGIKCFIHLKADVVSTLRD
                     LAKQMEVLK"
BASE COUNT          766 a          619 c          453 g          902 t
ORIGIN      
        1 taagtattcc gcaccatata ttctttcaaa atatcattct gaatagttcc attcaatgcc
       61 tggggcggca caccctgttc ttctgcagca acaatataaa aagccataat cggaattacg
      121 gctccgttca ttgtcattga aacactcatc tgatcaagcg gaatctggtc aaaaaggatt
      181 ttcatatcca gaatggaatc tactgccact cctgcttttc ctacatctcc aatcacgcgt
      241 ggatggtctg aatcgtaacc cctatgtgtc ggtaaatcaa aggcgatgga aagacctttc
      301 tgtcccatag ccaaatttct gcggtaaaag gcattgcttt cttcggctgt ggaaaaaccg
      361 gcatattgac gaatcgtcca aggtctttga atatacattg taggataggg acctcttaaa
      421 aaaggcggta aaccggataa ataatccaaa tgttccgtag aaataatatc gtcttttgta
      481 tatagaggtt tcacttctat ctgctcattg gttttccagc tgaaattgag cttaggtttc
      541 ggcttttctg gagcgctaaa atcgggattt atttgcagaa aatcggggtt tttcatttta
      601 acacctccat ctgttttgcc aaatcccgta aagtagatac tacatccgct ttaaggtgaa
      661 taaaacactt tattcctgcc tgcttatatt cctcaatttt atctgccgga taaccagcta
      721 aaattaatat cttacccttc attttagcac atatttcggg aactaagcta acataattct
      781 catccgtgga acaaatacaa aaggcactgg caccagattt ttctgctgct gttactgctt
      841 cctcaactgt attaaagcca ttgggataaa taacttccaa accacccaca ggaaagaaat
      901 tgagggcaaa atcagctctg gctttgtagt cagccaaagt tcccagattc agcaaaaata
      961 tctttttatt ggtttggctt gcttccatct gcattcttaa attctccatt tcttctacag
     1021 ctctatgttt cggtaatgct ccatctttca gaataaaagc tttatcttct tttgaaacag
     1081 gtttatcatc cctttgtaag gtaggcaatt tctcttcagg attggcatac atattaatgc
     1141 ccacaaagat gtttttcctt ttattcactg cctctattct ttccctaaca attgtttcaa
     1201 cttcctgatg aatttctcct ttagttacat attccagcat tccacctttg ccttcaattt
     1261 cctgcattag ttgccaggca ctttttgcca tttcattggt tagccattct atatagtaac
     1321 aaccccctgc tgggtcaatc actttatcaa aatgcgcttc ttcttttaaa ataatctgct
     1381 gatttcttgc taaacgagca gaaaattcat ctggctgagc aattagttca ttaaaacagc
     1441 caatttccaa gctatctaca cctccaataa ccccggcaaa gctctcggtc gttgttctta
     1501 acacatttac atagatgtcg taaattgtct tgttgaattt agctgtcttc ccgtgaatcc
     1561 aaaccttctg tttatcctct tccccaccga aggacttaat catttccgcc caaagtaaac
     1621 gcagagctcg cactttggct atttccataa agaaattgga accaagagct aattttactt
     1681 gcaataaagg tgctacctgc tctatcgtcc aaccggaatt cagtaattgt cttaaataat
     1741 aaattgcagt agctaaagca aaagccagtt cctgaacact ggaagcacct gcctcttcat
     1801 aaacagaggt atctatgctg ataatcctgt tccggggcat tttttctaca ttccatttta
     1861 ctatttctgc caatttctgt tccgcatccg ccaaagtgca aggcagataa cctttttctg
     1921 ccaattcacc gatataatcc agtcctatgg ctgattccaa ggatttcagc tctatattat
     1981 gtttgacagc atattgtaca aatgttatta gaatttccgt atcctcaatc cccaattgca
     2041 taaagagcgg agcggcttgc agattgattc cttccaaaag tgtttcccaa tcagccaagt
     2101 tctgtatttt taccccttcc gtggagattt tattactcaa ggtaatattt acagccgtca
     2161 agccctgatt caattctttc agcagttttt ggtttagtat cttcgggtct gtatctgtat
     2221 ggttttgagc aattaaccac ccctcagtta aaaaacggag gggattattg ccacgcacaa
     2281 aaggactgct tcccggttgg cttcctaaaa aaggaatacc ttctatgtct tctttacgat
     2341 agattggttg cagggttatt ccttccgtgg ttttggtttt cagaacttta tcaaagtccg
     2401 cacctttcag tcctgcttgc actacctgta gccattcctc aaaactggga ggaggaaatt
     2461 cctgcagcag gttcaactta atttcactgt ccactatttt ctcctttttt atattagatg
     2521 aacgacatcc ttgtcgttcc ttaattcttt agccaccgaa aatatataag cacgcagatt
     2581 tcgccgataa cgcagattaa aaaaagataa taggtctgga atgaacagga ttaaagggat
     2641 ttaagagatt tataatatag aatgggtctt gataaaataa caaggcacct tctaattatt
     2701 ccataccagt cctatcttaa ttatacattt ttcattgaac
//
LOCUS       DAOPFL010000156         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_51976_length_2736_cov_108.965684, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000156 DAOPFL010000000
VERSION     DAOPFL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51976_length_2736_cov_108.965684"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            605..1858
                     /gene="hutI"
                     /locus_tag="PLG69_07400"
     CDS             605..1858
                     /gene="hutI"
                     /locus_tag="PLG69_07400"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPI43366.1"
                     /translation="MNADVIIINISQLVTLANNSKPRYGKEMEESAIITDAGIAIKGG
                     NIIEINDSKAIGNKYLTSEIIDAEGKIATPGFVDCHTHPVFVHTREDEFAMRLQGKSY
                     VEIAKAGGGILSTIQTTRNADEDLLFELAKKRILKMMQLGTTTLEAKSGYGLNTPSEL
                     KQLRVIKRLQEELPIDIVPTFLGAHEFPEEYRNNHSGYVELLCKEMIPAVAEQGIAEF
                     CDIFTEAHTFNIEESRQILTCAGEHGLKLKMHCDEIEPIGGAELAAELKCISADHLGS
                     ASETGIKALKKAGVIPVLLPATLFSLQSKTYANARLMMDLGLPVAIATDFNPGSCNCD
                     SMQFTMSLSCLQMGLNPSSALCSATLNSAYALDRGNQIGSLEAGKKADIILWDIPGIN
                     FIPYHLGSSYVDRVLKCGKTIYKAT"
     gene            1859..>2736
                     /locus_tag="PLG69_07405"
     CDS             1859..>2736
                     /locus_tag="PLG69_07405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPI43367.1"
                     /translation="MPETTLNSYQIIKTVHSSLRFSLYQAKQMETGQIVLLKTPDTQR
                     LDDEKLKQDLINEAHTHLKLSHPQIRKVYEIREEKGTAYLIGEYIEGMSLSAYLKINS
                     DSLPIELTLSWLKELLEALSYAHKQDCIHLNLNPYNLIVDNANAMHIIGFGKNQDAYK
                     TANEKNGTYHPLLYLAPELFQAGIALPQSDLYSFAVIAYQILCGVVPWRIDLQLSPEK
                     QKQQSLCRAVIMPEILHKQVPDWLFTALLQCLKLDPHQRFANAEELITVFNEQENWLP
                     KEDEKVESVQKVEKIE"
BASE COUNT          870 a          519 c          549 g          798 t
ORIGIN      
        1 atttttatcc agtaaatata taaatgcata ggtgtcaagc ataaatgttt attttacacc
       61 gagaacactg aagttaccta aaataacgaa agcgttgtcg gagaacagtg tttttctaga
      121 agattgaagt cctattcaac caaataaact tcacgcagat tgcgctgatt acgcagatat
      181 ttttattttt ttattacaga tattaaatcc tgctgatccg gttgatccag ttatctgcgt
      241 tctattatta tattttttag ggtcgtcata agtcctcact gccaaatcaa tttgtaatag
      301 gtgtaaaatt tttgaaggca gagacgactc cttacctgca attatttcgt cagtgattag
      361 ctcttacttt ccgaaaataa tttcagggga aaacacaggg aagtgagtaa aagcataaca
      421 tactgaaatt atgtaaaata gaagttcttc cttaggagca ttacttaccc attgcttacc
      481 cattgctaat gccatccgta atgggtaagc aatgggtaag caatgctccc tgctgaaaaa
      541 gcttgacgga atattttagg aattacataa tgtatcttta agattgctaa agtatgagga
      601 aagaatgaac gccgatgtga ttatcatcaa tatcagccag ttagttactc ttgccaataa
      661 tagtaaacca cgctacggca aagagatgga agaaagtgct attattaccg atgcggggat
      721 agccataaaa ggtggcaata ttattgaaat aaacgacagt aaagcgatag gaaacaaata
      781 tctaacttcc gaaattatag atgccgaagg gaaaattgct actcccggtt ttgtggattg
      841 tcatactcat cccgtttttg tccatactcg cgaagatgaa tttgctatgc gtttacaggg
      901 aaaaagttat gtggaaatag ccaaagcagg aggtggcatt ttaagcacaa ttcagacaac
      961 cagaaatgca gatgaggatt tgctttttga attggctaaa aaacgcatcc tgaaaatgat
     1021 gcagttaggg actacaaccc ttgaggcaaa aagcggttac ggattgaaca ccccaagcga
     1081 attgaaacaa ttgcgggtga tcaaacgctt gcaggaagaa ctcccgattg atattgtccc
     1141 tacttttttg ggtgcacatg aatttcccga agaatataga aataatcatt ccggctatgt
     1201 agaactcctt tgtaaagaaa tgattcctgc tgttgcggaa caaggaatag ctgaattttg
     1261 cgatattttt acagaagcgc atacttttaa tattgaggaa tcgcgccaaa tacttacctg
     1321 tgcaggtgaa cacggactca aattaaaaat gcattgtgat gagattgagc ccattggtgg
     1381 tgcagaactt gcagcggaac taaaatgtat atcagcagac catttgggtt ctgccagcga
     1441 aacaggaatt aaagcgctca aaaaagcggg agtgatacct gttttattac cggcaacttt
     1501 attttcctta cagagcaaga cctacgctaa tgcccgtctg atgatggatt tgggacttcc
     1561 tgttgccata gctacggact ttaaccccgg aagttgtaac tgtgacagta tgcaatttac
     1621 tatgtctctt tcctgtttac aaatggggtt aaatccttcc tctgctttat gttcagccac
     1681 tttaaattct gcttatgcgc tggatagagg taatcaaatc ggctctttgg aggcaggaaa
     1741 aaaggcagat attattcttt gggatattcc ggggattaat tttattccct atcatttggg
     1801 ttcttcttat gttgaccgcg tgctaaaatg cggaaagaca atttataagg caacttaaat
     1861 gccggagaca actctaaaca gctatcagat aattaaaacc gttcacagct ccttgaggtt
     1921 ttctctctat caggcaaaac aaatggaaac cggacaaatt gttttgctta aaacccctga
     1981 tacacaacga cttgacgatg agaaactgaa acaggatttg attaatgaag cccataccca
     2041 ccttaaactt agccatcccc aaattagaaa ggtctatgaa atcagagaag aaaagggaac
     2101 tgcttatttg attggagaat acattgaagg aatgtctctt tctgcctatc tgaaaataaa
     2161 ttccgattcc ctacccattg aacttaccct ttcctggcta aaggagcttt tagaggcact
     2221 ttcctatgcc cataaacagg actgtatcca tttgaattta aatccttata atctcattgt
     2281 tgataatgct aacgctatgc acataatcgg tttcgggaaa aaccaggatg cctataaaac
     2341 agccaatgaa aaaaatggca cctatcatcc tcttttatac cttgctccgg aactttttca
     2401 ggcaggaatt gctcttcctc aatccgatct atattctttt gctgtaatcg cttatcaaat
     2461 tctttgtgga gttgttccct ggagaattga cctgcaactg agcccggaaa aacaaaaaca
     2521 gcagtcactt tgccgggctg ttattatgcc ggaaattttg cataaacagg ttccggactg
     2581 gctttttacc gctttactgc aatgtttgaa acttgatcct catcaacgct ttgccaatgc
     2641 ggaagaactt ataacggtat ttaacgaaca ggaaaattgg ctgccgaaag aagatgaaaa
     2701 ggtggaaagc gttcagaaag ttgagaaaat tgagag
//
LOCUS       DAOPFL010000157         2717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_52464_length_2717_cov_78.623591, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000157 DAOPFL010000000
VERSION     DAOPFL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52464_length_2717_cov_78.623591"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..910)
                     /locus_tag="PLG69_07410"
     CDS             complement(218..910)
                     /locus_tag="PLG69_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPI43368.1"
                     /translation="MHIAFKNVSINGLLHNFNLNFTTDGCTVLYDPCEKYSSTILTAL
                     MGLYNLDEGNILLDGISLEEYCRQNSLISTFSLVFDEGIMLSNLSLCENLLLPWNKRF
                     ASEDQNAFEQELKLLMEELNLNCGWELRPAYLSPAERKFFCFIRSLLLKPKIMLIDDP
                     YYLFNKEERRLLLGFLQKHSKKRGGTEFSDYPKSENKEEIYLQEMLIGTSDDDFTGEI
                     ATQVIDLSEICY"
     gene            complement(910..1671)
                     /locus_tag="PLG69_07415"
     CDS             complement(910..1671)
                     /locus_tag="PLG69_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPI43369.1"
                     /translation="MQHNRKKSLGDFFLFLEETVTELLHIRHNRHVSFLVVIRQILFT
                     GVEALPLIGFIALAIGCLTIMQGYAFLSNFGQGIWVHIILVRVVVGELSGIITALVVI
                     ARSGTAISTELGNMVVNREMQLLKSFDIAPMGYLVVSRILGVVISMLVLTLYFNIIAV
                     LGGWLFAQFFSHLAFGAFMNDFLSVLTLPDIFMIILKSVIFGFVIAITASYEGMSVYR
                     ASTEVPQRTIKAVVISIFSVLILDIIISWLFWIGA"
     gene            complement(1699..2055)
                     /locus_tag="PLG69_07420"
     CDS             complement(1699..2055)
                     /locus_tag="PLG69_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43370.1"
                     /translation="MKKTMLILALMAILMLIATAGYAYKGSPVESFDITPNPMFKECV
                     ISLAVSVPLNINVQIQSCDGKVICDLYEGFVGKDIYLNWERTDNYGNYVPDGEYFVVV
                     NFSTRYTSLKKTLILK"
     gene            complement(2097..>2717)
                     /locus_tag="PLG69_07425"
     CDS             complement(2097..>2717)
                     /locus_tag="PLG69_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPI43371.1"
                     /translation="ENCNIPFAFDNDANLMALAESYTQKKNYVIGVTVGSGIGCGIVI
                     DGKIYHGAYGFAGELGHICIVDKGLRCNCGKNGCLEAYSSGEGLRRRLALKNPCYAEM
                     DLPAILAIKETDNLVGDYIKQGQLFLATALSGLATCLDPEIIIIGGGAMDLGLYYIEE
                     LEREIKKRLPPAHSIKLKVAKAINGNKAGVLGAIKLIEDKFNSKEE"
BASE COUNT          916 a          629 c          380 g          792 t
ORIGIN      
        1 cgaggggctt acgcacccat cgctatattt gtgtcgccct gacgggctct aaaggaatta
       61 acaaatctca accctctaaa ccttctaaac cctctcaacc ttctaaacct cctcaacctt
      121 ctaaaccttt taaaccctct caacctttta acccttctca accctctaaa cctcctcaac
      181 cttctaaacc ctctcaacct cttctattta gttctgttca gtagcagatt tcagataaat
      241 caataacctg cgttgctatt tcgcctgtaa aatcatcatc actggtgcca attaacattt
      301 cctgtaaata aatttcttcc ttattttcgc ttttgggata gtcggaaaat tctgttcctc
      361 ctcttttttt actatgtttt tgcaaaaaac ccaaaagcaa tcttctttct tctttattga
      421 acaaataata gggatcgtca atcaacataa tcttcggttt caagagcaaa gaccgaataa
      481 agcaaaaaaa cttgcgttct gccgggctta aatatgcagg tcttaattcc catccgcaat
      541 tcaaattcag ctcttccatc aaaagtttca gttcttgttc aaaagcattc tggtcctcgc
      601 tggcaaacct tttattccaa ggcagaagta aattctcaca tagggaaaga ttggaaagca
      661 ttatcccttc atcaaacacg agggaaaagg tggaaataag ggaattttgc ctacaatatt
      721 cctccagcga aatgccatcc agtaagatat taccttcatc caaattatac aatcccatta
      781 aagcagttaa aatggtagat gaatactttt cacagggatc ataaagcaca gtacaaccat
      841 ctgtggtaaa attaagattg aaattgtgta ataaaccatt tatagaaaca ttcttaaagg
      901 caatatgcac tatgcaccta tccaaaatag ccaggaaata attatatcca aaataagcac
      961 ggaaaaaata gaaataacca ctgccttgat agtgcgttgg ggaacttccg ttgatgccct
     1021 gtaaacactc atcccttcat aagaggcagt tatggctatt acaaagccga aaataacgga
     1081 tttgaggatg atcataaaaa tatcgggtag ggttaaaacg gataaaaaat cattcataaa
     1141 ggcaccaaat gccaaatgac taaaaaactg agcaaaaagc caaccgccta aaacagcaat
     1201 tatattgaaa taaagggtta ataccagcat agaaattact actcccaaaa tgcgcgaaac
     1261 aacaagataa cccatcggcg cgatgtcaaa ggatttcaac aattgcattt ccctgttcac
     1321 aaccatattt cccaactcgg tagaaatcgc tgttccactt ctggctatta ctactaaagc
     1381 ggttataatt ccactcaatt ctccaactac aaccctcacc aaaattatgt gcacccaaat
     1441 cccctgcccg aaattgctca aaaaagcata accctgcata atggttaaac agccaattgc
     1501 caaagcaata aaaccgatta aaggcagcgc ttccactcct gtaaacaaaa tttgccgaat
     1561 aaccactaaa aaacttacat gtctgttatg tctgatatgc aataactcgg ttactgtttc
     1621 ttctaaaaac aaaaaaaagt ccccaaggga cttttttcta ttgtgttgca taagttacat
     1681 cactgctcta aaagtttgtt atttcagaat aagcgtcttc tttaaggaag tataccgggt
     1741 agaaaaatta actacaacaa aatattcgcc atcaggaaca taattgccat agttatcagt
     1801 cctttcccaa tttaaataaa tgtccttacc cacaaaacct tcgtatagat cgcaaataac
     1861 tttgccatca cagctctgaa tctgaacatt gatattaaga ggaacagaca cagctaaaga
     1921 aattacacat tccttaaaca taggattggg agtaatatca aaactctcaa caggagaacc
     1981 cttataagca tacccagctg tggctatcaa cattaatatt gccatcagcg ccagaatgag
     2041 catcgttttt ttcattttat tcacccctta tttaatgcct aattaaaaag caagtttcat
     2101 tcctctttgc tattaaactt atcttctatc agttttatgg ctcctaaaac tcctgcttta
     2161 ttgccgttta ttgctttagc aacctttaat ttaatactat gtgccggcgg taatcttttt
     2221 ttaatttctc tttccagttc ctctatataa tataatccca aatccatagc tccgccacca
     2281 ataattatta tttccggatc aagacaggtt gcaagaccgg ataatgcagt agctaaaaaa
     2341 agttgaccct gtttaatgta atcccctact aaattatcag tttcttttat agcaaggatg
     2401 gcaggtaaat ccatttctgc ataacaagga ttttttaatg ccaaacggcg gcgtaaacct
     2461 tctcctgaag aatacgcttc caaacagcca tttttcccac aattacatct taatccttta
     2521 tccacaatgc aaatatgacc caattctccg gcaaaaccgt aagccccgtg ataaattttt
     2581 ccgtcaataa ctattccgca accgattcca ctaccgacag taacaccaat aacataattc
     2641 tttttttgcg tatacgactc ggctaaagcc atcaaattgg catcgttatc aaaagcgaaa
     2701 ggaatattac aattttc
//
LOCUS       DAOPFL010000158         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_55341_length_2613_cov_106.486317, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000158 DAOPFL010000000
VERSION     DAOPFL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55341_length_2613_cov_106.486317"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..809)
                     /locus_tag="PLG69_07430"
     CDS             complement(69..809)
                     /locus_tag="PLG69_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPI43372.1"
                     /translation="MAKQDYKELLNELDKNKLPVHIGFIMDGNGRWAKKRNRPHLYGH
                     RAGVKALRQVVELGVELKLQYLTFYAFSTENWSRPESEVKGLLRLLKDQLKKQIPELM
                     EQNIYAQFIGSTVGLEENYWKEVSSLVAQTHNNTGMIVNFAFNYGGRLEIIEGFKKFM
                     TMHPEEWHKLTPEEFGNYLWTAGQPDPDLIIRTSGEKRLSNFLLWQSAYAELYITDTL
                     WPDFDKVELIKALLDYTSRERRFGGRNE"
     gene            complement(802..1866)
                     /gene="prfB"
                     /locus_tag="PLG69_07435"
     CDS             complement(802..1866)
                     /gene="prfB"
                     /locus_tag="PLG69_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPI43373.1"
                     /translation="MKFGGYFDPEPKKHLIMELEQKMNAPDFWEDQTQAKKISKQLSQ
                     LRDELEHLKILDTVKGELETYLTLLDEDFNAELLAEAVSELPRIQSFIEKAEIECYLN
                     DKYDHNNALLTIHSGAGGTESQDWAEMLLRMYAHWAEKNDYSFDIIDSLPGEEAGLKS
                     VSVEIKGDYAYGMLKSEIGVHRLVRISPFNAQGKRQTSFASVFVYPEFDEDLEIEINP
                     KDLKIDTFRASGAGGQYVNTTDSAVRITHLPTNITVTCQNERSQMQNREKAMSILKSR
                     LYQYYEEQREKEKKNLENSKTEIGWGNQIRSYVFHPYQMIKDHRTNYETGNVDKVMNG
                     DLNDFIYAWLKYNAHQRING"
     gene            complement(2073..2474)
                     /locus_tag="PLG69_07440"
     CDS             complement(2073..2474)
                     /locus_tag="PLG69_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HPI43374.1"
                     /translation="MKKTKFGLLLGIGICAIIIVSCSAPETYSSKPVSQSTEQKTPEI
                     EKVSKVVNYHQDKLQAIYKKHTYQNAMQGDLEFTLYITNEGQVQEVEVKALSGKFFSN
                     FIDELKKEIFTWEFPKQDKIIYSFVVSFRKG"
BASE COUNT          749 a          543 c          414 g          907 t
ORIGIN      
        1 ccctataata cagaacgata agaactatgg gaattaaaat aactgcaacc agcactcttt
       61 ttgccagttc actcattcct gcctccaaac ctgcgttccc gggaagtata atccaaaagc
      121 gcctttatca gttccacttt gtcaaaatcg ggccacaatg tatctgtaat atagagttcg
      181 gcataagcgg attgccaaag caaaaaattg gaaagacgtt tttctccgct ggttctaatt
      241 atcaaatccg gatcgggctg accagctgtc caaaggtaat ttccaaattc ctcaggggtt
      301 agtttatgcc attcctcagg atgcatcgtc ataaactttt tgaagccctc aataatttcc
      361 aggcgacctc catagttgaa ggcaaaatta actatcattc ctgtgttatt atgtgtttga
      421 gcaaccagag aacttacttc tttccagtaa ttttcttcca agccaacagt agaaccaata
      481 aattgagcat agatattttg ttccatcagt tccggaattt gcttttttag ctgatcttta
      541 agcaatctta acagaccttt tacttcgctt tccggacggc tccagttctc tgtagaaaaa
      601 gcatagaagg ttaaatattg aagttttagc tcaactccca attctacaac ttgccttaat
      661 gctttcacac ctgctctgtg gccgtaaaga tggggtctat ttcttttttt tgcccatctt
      721 ccatttccat ccataatgaa acctatatga acaggtaact tgtttttatc cagttcattg
      781 agcaattctt tataatcctg tttagccatt tatcctctga tgtgcattgt atttcagcca
      841 ggcataaata aaatcattca aatcaccatt cattacctta tcaacattgc ctgtttcata
      901 atttgttcta tggtctttaa tcatctgata gggatggaaa acataactgc gaatttgatt
      961 gccccagcca atttccgttt tgctattttc cagattcttt ttctcttttt ccctttgttc
     1021 ctcatagtat tgatataaac ggcttttcaa aatggacatt gctttttccc gattttgcat
     1081 ctgactgcgt tcattttggc aggtaactgt tatatttgta ggtaaatgtg ttatcctaac
     1141 tgcagaatca gtggtattaa catattgtcc tcctgctcca cttgcacgga aagtatctat
     1201 tttcaagtcc ttggggttaa tctctatttc caggtcttca tcaaattccg gatacacaaa
     1261 aaccgaggca aaagaggtct gtcttttacc ttgagcgttg aaaggactta ttctcactaa
     1321 acggtgaaca ccaatttcac tttttaacat cccgtaagca taatcacctt tgatttcaac
     1381 gcttacactt ttaagtcctg cttcttctcc aggtaatgaa tcaataatat caaagctata
     1441 gtcatttttt tctgcccaat gagcatacat ccgtaaaagc atttcagccc agtcctggga
     1501 ttctgttccg cctgcaccag aatgaattgt cagcagtgca ttattatggt catatttatc
     1561 gttcagatag cattcaattt cagctttttc aataaaactt tgtatgcggg gaagttcaga
     1621 aaccgcttct gccagtaatt cggcattgaa atcttcatct aataaggtaa gatatgtctc
     1681 cagttcacct ttaacggtat ccagtatttt cagatgctcc agttcatctc ttaattgaga
     1741 aagctgtttg cttatttttt tggcttgtgt ttgatcttcc caaaaatcgg gagcattcat
     1801 cttttgctcc agttccatta tcaaatgctt tttgggctcg ggatcaaagt aacctccgaa
     1861 tttcagaaat ttgttctatc atatcattgg cggtcttttt ataatcagtg tattccacgc
     1921 tttcatcctc ttttttataa gttgtaacct aaagatttac atacacattg atgtcaagaa
     1981 gaaaggacaa gaatgaagaa tggggctttt tttatgtttc ctcgctctaa gtaattattc
     2041 cagaagaact ctctttttct ctttataaaa actcaccctt ttcggaaact caccacaaaa
     2101 ctatagataa ttttatcctg cttaggaaat tcccaggtaa aaatctcctt cttcagttca
     2161 tcaataaaat tggagaaaaa ctttccggaa agtgctttca cttctacttc ctgaacttgc
     2221 ccttcattag taatataaag ggtaaattcc aaatcgcctt gcattgcatt ttggtaagta
     2281 tgttttttat aaattgcctg caacttatcc tggtggtaat ttactacctt agagaccttt
     2341 tctatttccg gagttttctg ttcagtagat tgagaaaccg gttttgaaga ataagtttca
     2401 ggagcagaac aggatacaat tattatagca caaattccta tgcctaataa caacccgaat
     2461 tttgtctttt tcataaagca ctccttttat tcgttataga tatattaagg taaaatatca
     2521 taaaaccaaa tagttcttga cggtaactta ctatataaaa attggaaatc gtcacgactt
     2581 gcttttttta caaagagaaa agagaaaaag aga
//
LOCUS       DAOPFL010000159         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_55564_length_2605_cov_50.565882, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000159 DAOPFL010000000
VERSION     DAOPFL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55564_length_2605_cov_50.565882"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..870)
                     /gene="cas5"
                     /locus_tag="PLG69_07445"
     CDS             complement(91..870)
                     /gene="cas5"
                     /locus_tag="PLG69_07445"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HPI43375.1"
                     /translation="MNIPQNSNRILELKLSGKFAHFRKFYTNASSLTYLIPPRTAICG
                     LLASILQIERDCYYNLMSSDKLGIAISLVPNIYYHKVFQTLNYAQDKSKNIPINDLSA
                     HKQCRLELLKAQGTKNLEWILYLCFNSAEENNFAELENKIKEKNFGYGLYLGQRQFIA
                     HLELIRTYSQGEFKPISESDYLDSAIERELIEGITSQDCDLIMERMPLEQELIEGKRK
                     SYRQTKRFGNIVIETNGKRIYGKFRNLVELNNDLKARVSFL"
     gene            complement(883..1752)
                     /gene="cas7b"
                     /locus_tag="PLG69_07450"
     CDS             complement(883..1752)
                     /gene="cas7b"
                     /locus_tag="PLG69_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas7/Csh2"
                     /protein_id="HPI43376.1"
                     /translation="MINNNSEILFLYDAQMCNPNGDMDNENKPRMDYDTFINLVSDVR
                     LKRYIRDYFEDIKGEEIFISNKAKDAKERNKQIQDLKKGHLDLIDVRLFGAVTAEEDR
                     GGHFTGPVQFNWGYSLHPVEIVDSSTITSSFSGGQGIGKDYRLYYSLIAFSGSVNANA
                     AEETNLTEEDLKLFDEAMLNSIPLARTRTKIGQYPRLYLRIELKDKDKFLKDLRSMIK
                     VTSQNSNIAQNLRLIRNPEDYELNINPLIEYLNQNKELISCIHYWKDEQISIPDFDKI
                     FIQDDWKKPLSLK"
     gene            complement(1745..>2605)
                     /locus_tag="PLG69_07455"
     CDS             complement(1745..>2605)
                     /locus_tag="PLG69_07455"
                     /inference="COORDINATES: protein motif:HMM:NF021033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM1802 family CRISPR-associated protein"
                     /protein_id="HPI43377.1"
                     /translation="INGMIKNINLRELINKTEHLNQMVLDYKLGSLYGYQDALSFEGL
                     RYLILPSKESHPGLDQYDYTVLNKNIISTLEIYLYNHSFQYNKLIRQFIDIFNRKNNN
                     LETENKYFLNITPFFMTLYLDHLLKFNQLEGVHIMEKRNLIAALQDEKITAYFNTHSQ
                     IYENNFYAQGLFILGMYIAAIESEQRKKNIKSTLINRLNLRGIPVQKVKSLMAMVDEM
                     GKVWDIFYDSVTENYYRECMQGIEKSSLSPEEIIFHILCGRAYSNYLGAIYKQEHPDK
                     TEKNQEEQND"
BASE COUNT          812 a          433 c          405 g          955 t
ORIGIN      
        1 ggatcatttc tttagcatca gaagctactt cagctaaatg atctaccaaa agcttttcct
       61 gatatccgtt tttggcagga tgggaaataa tcacagaaaa gataccctgg cttttaggtc
      121 attatttaat tcaactaaat ttctaaactt accataaatt cttttcccgt ttgtttctat
      181 aactatattt ccaaagcgtt tggtttgacg ataacttttt cttttccctt caattaattc
      241 ctgttccaga ggcatccttt ccataattaa atcacaatct tgagaagtaa tgccttctat
      301 caattctctt tcaatagcag aatcaagata gtcagattca gagattggct taaattcacc
      361 ttggctatag gttctaatta attccaaatg ggcaatgaac tgtctttgtc ctaagtataa
      421 gccataaccg aaattttttt ctttaatctt attttccaat tctgcaaagt tattttcttc
      481 tgcactatta aagcaaagat aaagtatcca ttccagattc ttagtaccct gtgctttaag
      541 aagttccaaa cgacattgtt tatgcgcgga aagatcattt atggggatat ttttactctt
      601 atcttgagca taattcaatg tttgaaacac tttatgatag tatatgttag gaactaaaga
      661 tattgctatt ccaagtttat ctgaactcat tagattataa taacaatctc tttcaatttg
      721 taaaatagaa gccagtaaac cacaaatagc agttcggggt ggaattaaat aggttagaga
      781 agaagcgtta gtataaaatt tacgaaaatg agcaaattta ccgctcagct tcagctctaa
      841 aatccgattt gaattttgtg gaatattcat atctttcccc catcatttaa gggaaagcgg
      901 ttttttccag tcatcttgga taaatatttt atcaaaatca ggaattgata tctgttcatc
      961 tttccagtaa tgtatgcagg aaatcaattc tttgttttga ttcaaatatt ctattagagg
     1021 attaatgttt aattcataat cttccggatt tcggataaga cgaagatttt gtgctatatt
     1081 agaattctga gaagttacct taatcatact gcgtaagtcc tttaaaaatt tatctttgtc
     1141 cttcaattct atccgcaaat acaaacgtgg gtattgtcca attttagttc tggttctggc
     1201 aagagggatg gaatttagca ttgcctcgtc aaaaagtttt aggtcttctt cagtaagatt
     1261 tgtttcttct gcagcattgg catttacact tccggaaaag gcaatgagag aataatataa
     1321 acggtaatct tttcctatac cttgtccgcc ggaaaaactg gaagtgatag ttgaactatc
     1381 tactatttct acaggatgta aagaataacc ccagttaaat tgaaccggac ccgtaaagtg
     1441 accacctctg tcctcttcag cagttacagc tccaaaaaga cgaacatcaa taagatcaag
     1501 atgtcctttc ttcaaatctt gaatttgttt attgcgttct ttagcatctt tggctttgtt
     1561 ggaaataaag atttcctcgc ctttgatatc ttcaaaataa tctctaatat agcgttttaa
     1621 gcggacatcg gaaacaaggt ttatgaaagt atcataatcc atacggggtt tgttttcatt
     1681 gtccatatca ccattaggat tgcacatttg tgcatcgtaa agaaatagga tctctgagtt
     1741 attgttaatc attttgttcc tcctgatttt tttcagtttt atctggatgt tcttgtttat
     1801 aaatggcacc taaataattt gaataagctc tgccacataa aatatggaag ataatttctt
     1861 ccggggatag agaagatttc tctatgcctt gcatacattc tctgtaataa ttttcggtta
     1921 cagaatcata aaatatatcc caaaccttac ccatttcatc taccatagcc atcaaggact
     1981 taactttttg aacagggatt cctctcaaat tcaatctgtt aattagagta cttttgatat
     2041 tcttttttct ctgttcggat tctatagcag caatatacat tcctaagatg aaaaggcctt
     2101 gagcataaaa gttgttttca taaatttggg aatgtgtgtt aaaataagca gtgatttttt
     2161 catcctgcag tgcggcaatt aagttacgct tttccataat atgaactcct tctaattgat
     2221 taaattttag taaatgatct aaatataagg tcataaaaaa gggtgtgatg tttagaaaat
     2281 atttattctc ggtttctaaa ttattgtttt tccgattaaa gatatctatg aattggcgaa
     2341 taagtttatt atattggaag ctatgattat ataaatatat ttctaatgtg ctgataatat
     2401 ttttattcaa tacagtataa tcatattgat ccaaacctgg gtgagattct tttgagggta
     2461 aaattaaata tctgagtcct tcaaatgata atgcatcttg ataaccatat aatgaaccta
     2521 atttataatc cagaaccatt tggttcaagt gttctgtttt atttattaat tcccttagat
     2581 tgatgttttt aatcatacca tttat
//
LOCUS       DAOPFL010000160         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_56407_length_2576_cov_128.893693, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000160 DAOPFL010000000
VERSION     DAOPFL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56407_length_2576_cov_128.893693"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PLG69_07460"
     CDS             <1..882
                     /locus_tag="PLG69_07460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPI43378.1"
                     /translation="NFIAVNPAWCKALGWTEEETNTLNLKDITLAEEQQTLNQELSKL
                     AEGKVCSLRKTQRYKRKDGSFFWADLYRSALFDDKGKVMGILDIFVDIDVQVRSEKVQ
                     GELFQSLETLNKELSNANIGLKRQAKYDSLTGLYNRWVMEEMLNKEIIHSLETNRGFA
                     VGIADIDDFKKVNDTYGHDCGDLVLVKLTQSFLQKIRVTDSVCRWGGEEFLFLFSETT
                     ESGAMIVVERIRKSIEELNIDYKGNLIKVTITIGVSFYNGQDHLDSKELVDRADKAMY
                     EGKNSGKNKAVLYSNIP"
     gene            1031..1606
                     /locus_tag="PLG69_07465"
     CDS             1031..1606
                     /locus_tag="PLG69_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43379.1"
                     /translation="MKTNSRSWQPTLTLARLFEEQNQFYDALAAYELISQNDPSPELR
                     ERIEDLHLRILNDPNRTYDPRLEQLFSPEELAYLKILDHQGFLNMAKAREKLMEEMQG
                     SELIFSEVMPEDVLVDESELDTLREILEEIEQQAQLSLISETGDVGEYNVQDFLIAML
                     AKFDKDQKLSEVKLSDLLSLFLEMQSLKNQP"
     gene            1606..1773
                     /locus_tag="PLG69_07470"
     CDS             1606..1773
                     /locus_tag="PLG69_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43380.1"
                     /translation="MKQKHTLNINSLCASCYEKCKQKDLVLIITCPFYDPFPVQLELK
                     FKGLRKKPKNK"
     gene            1859..2431
                     /gene="rsmD"
                     /locus_tag="PLG69_07475"
     CDS             1859..2431
                     /gene="rsmD"
                     /locus_tag="PLG69_07475"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPI43381.1"
                     /translation="MRIITGIYKKRNLFLVPGMSTRPTSSFNREVIFSVLQDYAGCRV
                     LDLFAGSGSLGLETLSRGAVWVDFVEFAPSAINTILQNINLLGCSENCHLWRKKVDVY
                     LKSCENKYDIIFIDPPYNKNLINPTLKLIYEKSLLNEEGSIIVEHSPQEIIAEEFLPY
                     ITKEKYFKRSHFTWLRARNNDLNQESSLPK"
BASE COUNT          844 a          420 c          526 g          786 t
ORIGIN      
        1 aattttattg ccgtaaatcc tgcctggtgt aaagccctgg gttggacgga agaagaaact
       61 aataccctta atcttaaaga tataactctt gctgaagaac agcagaccct aaatcaggaa
      121 ctgagtaaat tagctgaggg aaaagtttgc agtttgcgta aaacacaacg ctataaacga
      181 aaggatggca gttttttttg ggctgatctt tatcgttccg cattgtttga tgataaaggc
      241 aaagttatgg gcattctgga tatttttgtg gatattgatg ttcaggtgag gtccgaaaaa
      301 gttcagggag aattgtttca gagtttggaa actttgaata aggaactgag caacgccaat
      361 ataggcctca aacgacaggc aaaatatgat tctctaaccg gactttataa cagatgggta
      421 atggaagaaa tgctgaataa agaaattatc cactctctgg aaactaaccg cggttttgct
      481 gttggaattg ctgatattga tgattttaaa aaggtaaatg atacttatgg tcacgattgc
      541 ggagaccttg ttctggtaaa gctaactcaa tcctttttgc agaaaatcag agtaacagat
      601 tcagtttgcc gttggggtgg agaagaattt ttgttccttt tttccgaaac gacggaatca
      661 ggagcaatga ttgttgtaga acgtatccgg aaaagtattg aagaacttaa tattgactat
      721 aagggtaacc tgattaaagt tacaattact attggggtta gtttttataa cggacaagac
      781 catctggata gcaaagaact tgtagacaga gcagataaag caatgtatga aggtaaaaac
      841 agcggaaaaa ataaagcggt gttatattca aatattccct gattttttca ctatatacgg
      901 attttacaat gccttgtaaa taaatctcgg tgttttcgct gttctcggtg taaaaaaatc
      961 actaaagaaa gcagttgaca taaaacaaag ttcttctatt gtgtctctat gaaataatgg
     1021 gagatagtaa atgaagacaa attcacgcag ctggcaacct actcttactt tagccagatt
     1081 atttgaagaa cagaatcaat tttatgatgc attagctgct tatgagctaa tcagccaaaa
     1141 tgatccttct ccggaattac gggaaaggat agaggacctt catctgcgta ttttaaacga
     1201 tcctaataga acttacgatc cccgtttgga gcaactcttt tctccggaag aacttgccta
     1261 tttaaaaatc cttgaccatc agggttttct caatatggct aaagcacggg aaaagctaat
     1321 ggaagaaatg cagggttcag aattgatttt tagtgaagtt atgcctgaag atgttttagt
     1381 tgatgaatcc gaacttgata ccttaagaga aattctggaa gaaattgaac agcaagcaca
     1441 gctaagttta atttccgaaa caggtgatgt tggcgaatac aatgttcaag atttcttaat
     1501 tgcgatgctg gctaaatttg ataaagacca aaaactgagc gaggttaaat tgagtgacct
     1561 gttatcgcta tttctggaga tgcagagctt aaaaaatcag ccctgatgaa gcaaaaacat
     1621 accttgaaca tcaattccct ttgcgcaagc tgttatgaaa aatgtaaaca aaaggatttg
     1681 gtgcttatta ttacctgtcc tttttatgat ccttttcccg tgcagctgga attgaaattt
     1741 aagggattgc gtaaaaaacc caagaataag tgataattcc tgctggcgca ggaatgacaa
     1801 taaaaaacac aggaaagaag aaggaatttt cattctaata aaaggatatg agtcccaaat
     1861 gcgtattata accggtatct ataaaaaaag aaacctgttt ttagttcctg gaatgagtac
     1921 cagacctacc tcttctttca atcgggaagt tattttcagc gttcttcagg attatgcagg
     1981 ttgcagggtt ttagaccttt ttgccggaag cggttcttta ggtttggaaa cactttcacg
     2041 aggagctgtc tgggttgatt ttgtagaatt tgctccttcc gctattaaca ctattttgca
     2101 gaatataaat cttttaggtt gttccgagaa ttgtcatctc tggcgaaaaa aggtggatgt
     2161 atatttgaaa tcctgtgaaa ataaatacga tatcatattt attgatccac cttataacaa
     2221 aaatttgatt aaccccactc tgaaacttat ctatgaaaag tcactactga atgaggaagg
     2281 tagcattatt gtggaacatt cccctcagga aatcatcgca gaggaatttt tgccctatat
     2341 tacaaaggaa aagtatttta agaggtctca ttttacctgg ctgagggcaa ggaataatga
     2401 tttaaatcag gagtcgtcac tgccgaaata acttttaacc tattcacgaa tttgtttggc
     2461 atttaggact aatgacgact ggtttcaagc agattaaaaa tatgaatttt tagccaccga
     2521 agccaccgaa aaaattcaat agttatcatt tttctctgtt ttctcttttt ctcttt
//
LOCUS       DAOPFL010000161         2568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_56636_length_2568_cov_71.467967, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000161 DAOPFL010000000
VERSION     DAOPFL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56636_length_2568_cov_71.467967"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLG69_07480"
     CDS             <1..227
                     /locus_tag="PLG69_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPI43382.1"
                     /translation="GLEAAKAGGIYPTVINSAVEAVSVLFLQNKIPYLKMFDLVKKAL
                     DEQEPILDPDLETILQINAQTYQKVLQQTE"
     gene            825..1967
                     /locus_tag="PLG69_07485"
     CDS             825..1967
                     /locus_tag="PLG69_07485"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HPI43383.1"
                     /translation="MKTPKVSINPAILEWGITRAGIPLEKVEKKFPNINLWIAKQERP
                     TLKQLEDLSTTLHIPFGYFFLENPPIEQIPIPLFRTKKEIQNNQFSPEFIDTLYDMQR
                     RQIWLKEYLIDNRYEKLPFIGSCNINDDPAQVSTQMREKLGIQDDWASIFSTWEETLA
                     FLRDKVQEIGIMFEKNGVVKNNTHRPLNVNEFRGFVLVDDFAPLIFVNNADCKAAQMF
                     TIAHELAHLWIGESAAFDLREMQPAQDAKEIFCDKVAAEFLVPARLLQEIEFDINSEY
                     VFISLSKIFKVSEIVIARRLLDCGYIDKKDFFSFYDGYCKRQMAQIKGGGDYYRTQSL
                     RVGKYFLQFVVQALNEGVLQYTDAYRLTGLYGRTFDNYVDKLLGNM"
     gene            1964..2494
                     /locus_tag="PLG69_07490"
     CDS             1964..2494
                     /locus_tag="PLG69_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HPI43384.1"
                     /translation="MILDNLVYVLDANVFITAHRTYYSFDIAPGFWKALLKYAESGKI
                     ISIDKVENELYKGHDPKNSKMPDKLAIWAKEKFKPYFKSTATEDVIKEYKEIVQWVSE
                     NNYYSQAVKNEFASSDLSDSWLVAFAKAYDNVLVTQEKPSKTKAKVPIPNLCEQFNVP
                     YVDTFQMLRRLGIILN"
BASE COUNT          880 a          401 c          481 g          806 t
ORIGIN      
        1 taggtttgga agcagctaaa gcgggaggaa tttatcccac cgttatcaat tccgcagtgg
       61 aagcggtttc tgtcctcttt ttgcagaata aaattcccta tctgaaaatg tttgatttgg
      121 ttaaaaaggc attggatgaa caggaaccta tccttgatcc cgatctggaa accattctgc
      181 aaatcaatgc tcagacctat caaaaagtcc tacagcagac agaatagaca gatggccgat
      241 atacgagaac tgcttttctc gtaacacaat gcttgttttg actttcaatt ttgccactgc
      301 catttcatta tacggttgtt actcattact tttcttgcaa ctgcattttg gctattttac
      361 taacttcaaa attgagagtt aaaacaagaa caaagaacac aaccaaccaa actacaacta
      421 tttcaagaac aacacagtaa cgaatgaact cttcatttgt acggtaacac aacaagagtg
      481 tagttttttt ctacaggttg aaggatgctc tgcaataaaa aaattagtta ccagcatctc
      541 ccattgaaat gaagttttgt tttaactcta aaccgagctt cagctttgcc aggaaaaggc
      601 agggaattaa atatcttgtt gccttacaaa tcccgtttca acacaaaaaa ggatcggtgt
      661 agagtaaaaa aagcacgaga gcatataaag aaaaaaaaca cttatatacc aggatattgg
      721 gaattttgta atagaaatgt tgaaattgta tttgacagaa aacattggtg tgattataag
      781 agaatttgtg taagtagtgt ttatttaaga aaggatatgt taaattgaaa acgccaaaag
      841 tttctattaa tccagctatc ttagagtggg gtattaccag agccggtatt ccattggaaa
      901 aagtggagaa aaagtttcct aatattaatc tctggatagc taaacaagaa agaccaacct
      961 taaagcaact ggaagattta tccactactt tacatattcc ttttggttat ttttttttag
     1021 aaaatccccc tattgaacaa atacccatac ccttatttag aactaaaaaa gaaattcaaa
     1081 ataaccaatt cagtcctgaa tttattgata ccctatatga tatgcagcgc aggcaaatct
     1141 ggctgaaaga atatctaata gataatcgct atgaaaaatt gccttttatt ggctcttgca
     1201 atataaatga tgatcctgct caagtttcta ctcaaatgag ggaaaaactg ggtatccagg
     1261 atgattgggc aagtattttt tctacttggg aagagacatt agctttctta cgagataaag
     1321 ttcaagaaat aggaattatg tttgaaaaaa acggagtggt aaaaaacaat acacatcgtc
     1381 cgcttaatgt taacgaattc cgcggttttg ttttagtaga tgactttgca cctctgatat
     1441 ttgtaaataa tgccgattgt aaagcagctc aaatgtttac tatagctcat gaattagctc
     1501 atttatggat tggagaaagc gctgcttttg atttgagaga aatgcaacct gctcaagatg
     1561 ctaaagagat tttttgtgat aaagtagctg ctgaatttct ggttcctgcc aggttactgc
     1621 aagaaattga atttgatata aactctgaat atgtttttat ttccttaagc aaaatattta
     1681 aggtcagtga gattgttata gcacgaagat tattggactg tggttatatt gacaaaaagg
     1741 attttttctc tttttatgac gggtattgta aaagacaaat ggcacaaatt aaggggggag
     1801 gtgattatta tcgtactcaa agtttgagag tgggcaaata tttcctccaa tttgtagttc
     1861 aagcgctaaa tgaaggagtt ttacaatata ctgatgctta tcgacttact ggactttatg
     1921 gtaggacatt tgataactat gtagacaagc tattggggaa tatgtgatat tggataatct
     1981 ggtttatgtt ttagatgcca atgtttttat aacagctcat agaacttatt actcttttga
     2041 tattgctcct ggattttgga aagcactgct taaatatgca gaaagtggta agataataag
     2101 tattgataag gttgaaaatg aattatacaa aggtcatgat ccaaaaaatt ccaaaatgcc
     2161 cgataaattg gcaatttggg caaaggaaaa atttaaaccg tatttcaaat caacagcgac
     2221 agaagatgtt attaaagaat ataaagaaat tgttcaatgg gtgtcagaaa ataattatta
     2281 cagtcaagcc gtaaaaaatg aatttgcttc gtctgatctt tctgacagct ggctggtggc
     2341 ttttgccaaa gcttatgaca atgttttagt gacacaagaa aaaccaagta agaccaaggc
     2401 aaaagttcct attcctaatt tatgcgagca gtttaatgtt ccttatgttg atacttttca
     2461 aatgctacgc cgtttgggca tcattctaaa ctgaaaaagt tgagagaaga ttcaagtttt
     2521 gttttaaccc taaaccggac tttacagcgg aaattttttt acagagaa
//
LOCUS       DAOPFL010000162         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_56676_length_2567_cov_104.782245, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000162 DAOPFL010000000
VERSION     DAOPFL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56676_length_2567_cov_104.782245"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..588)
                     /locus_tag="PLG69_07495"
     CDS             complement(217..588)
                     /locus_tag="PLG69_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43385.1"
                     /translation="MTAKRFYIAVLMGIILGIIACLIAGGVSPDPLAKKVLANIFVSR
                     VLIGFVIGISAWKMGWFLHGVLMGLIVGLPFSLSAMAIQVPGMGKWGIFFLTWALGVV
                     YGFIIELVTSVFFKAKQPSAK"
     gene            complement(769..2088)
                     /locus_tag="PLG69_07500"
     CDS             complement(769..2088)
                     /locus_tag="PLG69_07500"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPI43386.1"
                     /translation="MNNFNPVELLIKKRDGYSLAPEEISCFVNAYLKGEIADYQMSAF
                     LMACFLQGLDKQEIEALTKTYIDSGGTISFAEGLPVADKHSTGGVGDKISLMLAPIAA
                     SLGLMVPMISGRGLGHTGGTLDKLESILGFRTSFSLQEFKELVEKHSFALAGQSEELV
                     PADKKIYALRDVTATVESPGLITASIMSKKIAEGAKHLVIDLKIGSGAFMPNINKAKE
                     LAELLMATGKSFGQKVTVVFSNMNSPLGHAVGNALEMIEAIEYLKGNYLPDTYELTTV
                     LTAQMLISTGKAKNFEQAKKMIDEAVVSGKALEKFREIIIAQGGEPKVLEDYSLFASS
                     PVIYPIIAKQSGWVHKIDCRAIGYALVHIKAGRKKVTDTLDYGAGALLYPKIGDTIKQ
                     GEKIGEVHCASKNEAEEVAELILNAYKIADTQKDKEDLILEIALSEE"
     gene            complement(2100..>2567)
                     /locus_tag="PLG69_07505"
     CDS             complement(2100..>2567)
                     /locus_tag="PLG69_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPI43387.1"
                     /translation="IRTISTVFRSIEAIVWAIIFCVWVGIGPFAGMLALWIHSIAALI
                     KLYSEQIENIDPGPVEAIKATGASTLQVWRYGVTPQILSPYLAFTIYRWDMNIRMATV
                     VGFVGGGGIGLALYQEQQMLAWRNVGLIMWLIALIVWVMDMLSGYLRSKLVTY"
BASE COUNT          785 a          581 c          416 g          785 t
ORIGIN      
        1 ttcgaacccc cgacctagtg gttaacagcc acccgctcta ccgctgagct attccggaac
       61 acttttcaag aatacctaat ttttaaggtc gtgatttttg tcaatccaaa ttttttttgt
      121 agccggagct cgctgaagct ccgctttttt agtgattttt ttgcggagga atggcgtcct
      181 ctggctactc ttggggaaat gaactaaatt cgctatctat tttgccgagg gttgttttgc
      241 cttaaagaaa acgctggtta ccagctcaat gataaatccg tatacaactc ccaaagccca
      301 ggtcaggaaa aatattcccc atttccccat acctggaact tgtatggcca tagcacttaa
      361 ggaaaaaggc aagccaacaa tcagtcccat taaaactcca tgtaaaaacc agcccatttt
      421 ccatgcacta attccaatca caaaaccaat taaaacacgg cttacaaaaa tattggcaag
      481 cactttctta gctaaggggt cagggcttac tcctcctgct attaagcagg caataattcc
      541 caatatgatc cccattaaaa cggcgatata aaaacgtttg gcagtcatat taacctccta
      601 atactgtgct atttatatta tatagcaaga gcagggaaaa agcaaattat ttttacagct
      661 tttatataaa tgaatagtta tcgtcttgaa aaagcagtca agtcgtaata ttttacctaa
      721 attctgcttg tgcaggaatg acaaacgact tttctttcct aatatgattt attcttcaga
      781 taaagcgatt tccaggatga ggtcttcttt atctttttgg gtatccgcta ttttataggc
      841 attcaaaata agttctgcta cttcttccgc ctcattttta gaggcacaat gcacttctcc
      901 tattttttct ccttgtttta tagtatcgcc aattttagga taaagtaaag caccggctcc
      961 ataatcaagg gtgtccgtaa ctttttttct gcctgctttg atatgaacta aggcatagcc
     1021 aatagctctg caatcaattt tatgcaccca accactttgt ttagctatga taggataaat
     1081 tacaggtgaa gaggcaaaaa gggaataatc ctccagcact tttggttctc ccccttgagc
     1141 aataattatt tcccggaatt tttccagtgc ttttccgctg acaactgctt catcaatcat
     1201 cttttttgcc tgttcaaagt tctttgcttt gccagtgctg atgagcattt gagcggttaa
     1261 aacagtagta agttcataag tatcgggtaa atagtttcct ttcaggtatt caattgcctc
     1321 aatcatttcc aaagcatttc caacagcgtg tcccaaagga gaattcatat tggaaaagac
     1381 cactgttact ttttgcccga aactttttcc tgttgccatc aataattcag ccagttcttt
     1441 tgctttattg atattaggca taaaagcacc gctacctatt tttaagtcaa taaccagatg
     1501 ctttgctcct tcggcaattt tcttgctcat tatgctggca gtaatcaaac ccggactttc
     1561 tactgtagcg gtaacatctc ttaaggcata tattttttta tcggcaggaa caagttcctc
     1621 agattgacct gctaaagcaa aactatgttt ttccaccagt tccttaaatt cctgcagaga
     1681 aaaggaagtt ctaaatccta aaatggattc cagtttatct aaagtaccgc ctgtatgacc
     1741 caaaccgcgt cctgaaatca ttggcaccat caatcctaaa gaagcggcaa taggagccag
     1801 cattaaactg attttatctc ctacgcctcc ggtagaatgt ttatcggcaa caggcagacc
     1861 ttctgcaaaa ctgatagtgc ctccggaatc aatataggtc ttggttaatg cctctatttc
     1921 ctgtttatcc agaccttgca gaaaacaagc cattaaaaaa gcgctcattt gataatcggc
     1981 aatttctcct tttaaatagg catttacgaa gcaagaaatt tcctccggag caagagaata
     2041 accatcacgc tttttgatta acagttcaac cggattaaaa ttattcattc tgcaatctcc
     2101 taataggtaa caagtttgga acgaaggtaa ccacttaaca tatccattac ccaaacaatt
     2161 aaagcaatta accacattat taaacctaca tttctccagg caagcatttg ctgttcctga
     2221 tataaagcca aaccaattcc tccaccacct acaaaaccta caactgtagc cattctgata
     2281 ttcatatccc aacggtaaat tgtaaaagcc aaatagggtg acaaaatttg aggagtaact
     2341 ccataacgcc atacctgtaa agttgaagca cccgttgctt ttatagcttc cacaggaccc
     2401 ggatcaatat tttcaatctg ctcactgtat aattttatca aagcagctat ggaatgtatc
     2461 cataaagcta acattccggc aaaaggacca ataccaaccc aaacacaaaa aataattgcc
     2521 cacacaattg cttcaatact tctgaagaca gtagaaatcg ttctgat
//
LOCUS       DAOPFL010000163         2545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_57327_length_2545_cov_146.623695, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000163 DAOPFL010000000
VERSION     DAOPFL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57327_length_2545_cov_146.623695"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLG69_07510"
     CDS             <1..503
                     /locus_tag="PLG69_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPI43388.1"
                     /translation="IMQRFKAGEIKILVSTTVIEVGVDVPNASVMIVEHAERFGLAQL
                     HQLRGRVGRGSAQAYCYLIEHSPVSEVAWQRLTTMTKTTDGFIIAEKDLELRGPGEIF
                     GYEQSGIPQFRFANLVRDQEILKLARQDAFEIVRADPDFELPENALLKKIYFSQFTDK
                     EKLILY"
     gene            1073..2005
                     /gene="trxB"
                     /locus_tag="PLG69_07515"
     CDS             1073..2005
                     /gene="trxB"
                     /locus_tag="PLG69_07515"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPI43389.1"
                     /translation="MYDVIIIGAGPAGLSAAIYSARSGLKTVVLERGLIGGQINVTEI
                     VENYPGFAESISGYELTDRMHRQAEHFGAEFRDEEVTALGMEGLCKIIETTENKYRTK
                     AVIFCTGAYPRRLNVPGEEKFTGHGVSYCATCDGALYRDKIVAVIGGGDSAIEEGIFL
                     THFARKVIVIHRRDELRAQKIIQERAFQNPKIEFVWNSVVQEIRGNTRVQELEVYNRK
                     TNSLSVIPVDGVFIYVGILPNNKLLESRIELDSAGFVITDDFMQTNVPGIYAAGDIRK
                     KVLRQVVTATSDGAIAGWSAEKWITENYSSPQTE"
     gene            2016..>2545
                     /locus_tag="PLG69_07520"
     CDS             2016..>2545
                     /locus_tag="PLG69_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPI43390.1"
                     /translation="MISSKEIRQQFIDFFLNKGHIFVPSSPVIPEDDPTLLFANAGMN
                     QFKSIFLGQKEITYKRVVNSQKCIRAGGKHNDLEEVGKDGYHHTFFEMLGNWSFGDYY
                     KKEAISWAWELLTEVWKIPKDKLYATVYKTDTEAFEIWKQETDIDPTHISYFDDKDNF
                     WEMGETGPCGPCSEIH"
BASE COUNT          820 a          432 c          574 g          719 t
ORIGIN      
        1 ccattatgca aaggttcaaa gccggggaaa taaaaattct ggtttctact actgttattg
       61 aagtaggcgt ggatgttccc aatgccagtg tgatgattgt agaacatgca gagcgttttg
      121 gtttggcaca attacatcaa ttacggggta gagttggcag aggaagtgct caggcatact
      181 gttatttaat tgaacattcg ccggttagcg aagttgcctg gcagcgttta accactatga
      241 ctaaaacaac ggatggcttt attattgccg aaaaagacct ggaactgcgc ggtccgggtg
      301 aaattttcgg ttatgaacaa tccggaatac ctcaatttcg ttttgctaat ttagtgcgag
      361 accaggaaat attaaaatta gccaggcagg atgcctttga aattgtgcgt gcggatccgg
      421 attttgaact gccggaaaat gccctgttga aaaaaatata cttctcccaa ttcactgata
      481 aagaaaagct tatcctctat tagtaaatac cttggcgtag ctgcagctta aagcaaggat
      541 ttgctactgc cgaaataaat tttatgctac ctactaatta ttttagcagt taggattcgt
      601 gcctgctata aaatgatgga tgaaacatac agaacatata ttacactgca aatagaataa
      661 gatttttata tttaagagaa gaattattaa ttataagtta gtgatgttag tgtaatctgt
      721 aagagaaaat gtgtcagagt gttacaagta gcatacactg cgaaagttat tgtagggaac
      781 aaaattgtaa agctgtaaat gattcttagt ataaaagctg agtttcagcg aaccctgttt
      841 atgcttaaaa cttgtctgct gaaaaaatga ctcccttatg attcccatat cgcgatatgg
      901 gaacgatatg ggaatcatat cggaaacata atggctatga gagaggataa aatatacaga
      961 gatcatttac gataaaagaa aattaggcgt agcaattcaa ttttccttga cgaaagaacc
     1021 cgccttagtg ttttagctac tattattcaa aacagctaaa ggatagaaca taatgtatga
     1081 tgtaattatt attggagccg gacctgccgg cttaagtgct gccatttaca gtgcgcgtag
     1141 cggtttaaaa actgttgttt tggaaagagg actgattggc ggtcagataa atgttacgga
     1201 aatagtagaa aattatcctg gttttgctga atctataagc ggttatgaac tgacggacag
     1261 aatgcaccgt caagcagaac attttggtgc ggaatttagg gatgaagaag taacagctct
     1321 gggaatggaa ggactttgta aaataataga aaccacggaa aacaaatatc gtaccaaggc
     1381 agtgattttt tgcacgggag cttatccgcg gcgcttaaat gttcccgggg aagaaaaatt
     1441 taccggtcat ggggtttcct actgtgcaac ctgcgatgga gcattatata gagataaaat
     1501 tgtagccgta attggcggag gtgattctgc catagaagaa ggaattttct taactcattt
     1561 tgcccgaaaa gtaattgtta ttcaccggcg tgacgaactt agagctcaaa aaatcattca
     1621 agagcgcgct tttcaaaatc ctaaaataga atttgtctgg aacagtgttg tgcaggaaat
     1681 tcgtgggaat acaagagtgc aggaattaga ggtctacaat cggaaaacca attctttaag
     1741 cgttattccc gttgacggcg tctttattta tgtaggtatt ttgcctaata acaaattgct
     1801 ggaatcccgt attgaactgg atagcgctgg ttttgtgata accgacgatt ttatgcagac
     1861 caatgttccg ggaatttatg ctgcgggtga catccggaaa aaggttttac gccaagttgt
     1921 aaccgccact tccgacggag ctatagccgg ttggagcgct gaaaaatgga taacggaaaa
     1981 ttactcttcc ccacaaacgg aataaaggaa ttaaagtgat aagcagtaaa gaaatacgac
     2041 aacaattcat tgattttttc cttaacaagg gtcatatttt tgttccttcc tcacctgtaa
     2101 ttccggaaga cgatcctacc ctgctttttg ccaatgctgg aatgaatcaa ttcaaaagta
     2161 tcttcctggg gcagaaagaa ataacataca aacgcgtggt taatagtcaa aaatgtattc
     2221 gtgctggagg aaaacataac gacctggagg aagttggaaa agacggctat catcacacct
     2281 tttttgaaat gctcggtaac tggagtttcg gtgattacta taaaaaagag gcaatttcct
     2341 gggcttggga actgctgacc gaggtctgga aaattccgaa agataaactc tatgctaccg
     2401 tttataaaac tgataccgag gcatttgaaa tctggaaaca ggagacagat attgatccaa
     2461 ctcatatcag ctactttgat gataaagata atttttggga aatgggggaa acaggaccct
     2521 gcggtccctg ttcggaaatt catat
//
LOCUS       DAOPFL010000164         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_58195_length_2517_cov_47.665719, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000164 DAOPFL010000000
VERSION     DAOPFL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58195_length_2517_cov_47.665719"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..>2517)
                     /gene="cas3"
                     /locus_tag="PLG69_07525"
     CDS             complement(145..>2517)
                     /gene="cas3"
                     /locus_tag="PLG69_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HPI43391.1"
                     /translation="KLLVDHLAEVASDAKEMIQKLSLNLTTTTKDKLTFVAEKIGIMH
                     DLGKASSYFQNYVRGGNRNNLTHHSYISAVITFFNFHSWKEYSSFAPNAFKCVQKHHS
                     NLSSFWGEDLNNAALIDETLTIYKSVWENINNDVDLKKLLEKHNIILPELSYSSFQFM
                     SDNLEEFSLNYSPTNIDEAVESFLIQNLLFSVLIDADKHNAAVKEYVFLEKLNPKLSF
                     SPAKIIEEKNKVQGALNTLRNQFIDNIRSNQSITPSQKLYTLTAPTGSGKTFACLEFT
                     DHIQSYEDNLRRVIYCLPYTSIIDQNYQEFENVLLSNLNENINLDYRFLVKHHHLVDY
                     SAITKDEPTYNWEELQKDILSIESWESACVISTFVQLFHSLIGNRNGMVRKLHNIINS
                     IILLDEVQNLPADYYPLLRIVFKVLAEKFDTYILSCSATQPYLYPDGKYIELCKKELF
                     SVPDFNRVKLYINLKEQSVDEFCQNFLEFNDAKSILIVMNTKRSALAVYNYLSSNYQS
                     SYQVFCLTTLHIPIHRQEIIEKINKILKETQSKVILVSTQLIEAGVDISFQRVYRDFG
                     PLDSIIQVAGRCNRNNELGELGGEMFLVNLQKDGRLLSHYVYDKYLLSKTKECLEQFT
                     SLESRQFSHIIDAYYQILDIGAKGEAILRAISDLNYDQNIHNQVPIADFKLISDDYAT
                     ETVYILCDETSTKAISSIIGYLDCLKNTKLPKEEIQKIKTALDKEYRSLNLYQINISP
                     KELAVIYDSYKVKKLNDHIYYIDSEFIEDFYECSTGFKLYSTAKSSCLAL"
BASE COUNT          862 a          399 c          393 g          863 t
ORIGIN      
        1 cggttgttat tatagccgca ttggaaataa caatagaaga tatcttttcc ggagaaagct
       61 tggttttcct atcctcaata ctcaactcaa acatttcatc ttttttgcgt aagtaactgc
      121 cgtaggtatt taagtggact tccatcagag agccaaacag cttgatttag ctgtagaata
      181 tagtttaaaa ccagttgaac attcgtaaaa atcttctatg aattcagaat ctatataata
      241 gatgtggtca tttagctttt taactttata ggaatcgtaa ataacagcaa gttctttggg
      301 tgaaatattt atttgatata agttcaaaga acgatattct ttatccagag ctgttttaat
      361 tttttgaatt tcctctttag gaagtttagt attcttcaga caatccaaat atcctattat
      421 agacgatatt gctttagtag aggtttcgtc acaaagaata tacactgttt ctgtagcata
      481 atcatcagaa ataagcttaa aatcagcaat gggaacttga ttatgaatat tttgatcata
      541 attaagatcg gatattgctc ttaaaatggc ttcaccttta gcgccaatat caagaatttg
      601 ataataggca tctataatat gagaaaactg acgactttct aaagaagtga attgctccaa
      661 gcattctttt gttttagata atagatattt atcatataca taatggctta ataacctgcc
      721 atctttttgt agattgacca aaaacatttc tccgccaagt tcacctaatt cattattcct
      781 gttacatcta cctgcaacct ggataatact atctaaagga ccaaaatcac gatataccct
      841 ttgaaaactt atatcaacac ctgcttcaat caattgggta gagaccaaaa ttacttttga
      901 ctgtgtttct ttcaggattt tattaatttt ttctataatt tcctgcctgt gtataggaat
      961 atgcaatgta gttaaacaga aaacttgata ggaggattga taattagaag aaagataatt
     1021 atatacagca agagcggagc gttttgtatt cattacaatt aaaatgctct ttgcatcatt
     1081 aaattccagg aaattttggc aaaactcatc tacagattgt tccttcagat taatataaag
     1141 tttcacacga ttaaaatcgg gaaccgagaa aagttctttc ttgcataatt ctatgtattt
     1201 accatcagga taaagataag gttgagtagc agaacaagaa agaatgtaag tatcaaattt
     1261 ttcagcaaga accttaaata caattcttaa taaaggatag taatcagcgg gcaagttttg
     1321 cacttcatcc aggagaatta tagaattaat aatattatgc agcttacgaa ccatcccgtt
     1381 tctattgcca attagagaat gaaacaattg aacaaaagta gaaataacac aggcagattc
     1441 ccaggattca atagacagga tatctttttg caattcctcc caattgtagg ttggctcatc
     1501 ttttgtaatt gcagaataat ctaccagatg atgatgtttt actaaaaatc tgtaatctaa
     1561 gtttatgttt tcattgagat tagatagtaa tacattttca aattcctgat aattttggtc
     1621 tataattgaa gtataaggaa ggcagtaaat aactcttctt aaattatctt cataagattg
     1681 aatatgatca gtaaattcta agcaggcaaa agtttttcca cttccggtgg gggcagttaa
     1741 ggtatatagt ttttgggaag gcgtaatact ttggttactt ctgatattat ctataaattg
     1801 atttcttaaa gtgtttaatg caccctgtac cttattcttt tcctcaatta tttttgccgg
     1861 tgaaaagctt agctttggat tgagcttctc caaaaataca tattccttaa cagctgcgtt
     1921 atgtttatct gcatctataa gaaccgaaaa taaaagattt tggattaaaa atgattctac
     1981 tgcttcatct atattagtag gagaataatt aagagaaaac tcctccaaat tatcactcat
     2041 aaattgaaac gaactataag acaattccgg taagatgata ttgtgttttt ccagcagttt
     2101 ttttaaatca acatcattat ttatgttttc ccatacactc ttatagatag taagtgtttc
     2161 atcaataaga gctgcattat taagatcctc tccccaaaaa gaacttaagt tactatgatg
     2221 cttttgtaca catttaaaag cattaggagc aaaagaagaa tattctttcc aagaatggaa
     2281 attgaaaaaa gtaataaccg ccgaaatata actatgatgt gttaaattat tacgatttcc
     2341 acctcggaca taattttgaa aataggaaga tgccttcccc agatcatgca taattccaat
     2401 tttttctgca acgaaagtta atttatcttt agtggtagta gttaagttca gggaaagctt
     2461 ttggatcatt tctttagcat cagaagctac ttcagctaaa tgatctacca aaagctt
//
LOCUS       DAOPFL010000165         2516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_58226_length_2516_cov_92.460382, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000165 DAOPFL010000000
VERSION     DAOPFL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58226_length_2516_cov_92.460382"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..863)
                     /locus_tag="PLG69_07530"
     CDS             complement(216..863)
                     /locus_tag="PLG69_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43392.1"
                     /translation="MKRFGIFLLIILLSTSLFAQSRGILTLKSFAVPGWSQISSNRNY
                     GYAMLTAEAVIIGSIYYFHSESKVLEQESYEYAIKFAHLSPDGYSDEFYKQLARYESS
                     GFDAGGYNNWVRQTAMNLYPDDPESQQQYINENAYGNDKYWYWDSSKHRSEYNKIRNR
                     SMDYDNYALVAGGILILNHLVSTIDVLRWTAPPKKANLSFNLQDKTPILQINYHW"
     gene            complement(874..1524)
                     /locus_tag="PLG69_07535"
     CDS             complement(874..1524)
                     /locus_tag="PLG69_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43393.1"
                     /translation="MKRKTCFIILLLLWGIIPLLALPKFVYPVSSLILPGTGELLLGH
                     NTRGATLMGVDLINIYAFIATDREIEMQKKNYMKFAELYAGVPYGMPDEHYQAVQNYP
                     NSDYYNDIQEMMARNYYLIYNYDPESFAEYISANTYQGNEEWNWQSEEKWEEYKHLRK
                     RHQKTKMNNQLALGLMLLNRGISIIDSSILSKKKHGEFYLAPLPANGAMLNYQIEF"
     gene            complement(1521..2489)
                     /locus_tag="PLG69_07540"
     CDS             complement(1521..2489)
                     /locus_tag="PLG69_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43394.1"
                     /translation="MKKFILLIFIAGFAFCLFSQEKPKTDIDQWLIQTEEEQQNAMRD
                     SVVTNDLIKKNYNRKNAQLAMAMSMLVPGAGQFYANKTVLTTYIFPVLEIGLIGGLVY
                     FNNQGKDKTKKYEKYANGEIVTYITPDGTKITGFRYDRSRQDEIQAILIGVNQHDIYD
                     NGYFRLDKTNSQHFYEDIGKYPQYVFGWMDWYYRFACDESGVRDSIRWKFDGENDSPN
                     MRWTGNYPLWGNDTETLIPIDSHEMSRMRQTYIKMRNDAKDSYSMAHFFTLGLAFNHL
                     AAGLDAIRVTRNFNRYYISQSVPSLNFYAAMPLGNVTPMLGLKWTF"
BASE COUNT          791 a          496 c          408 g          821 t
ORIGIN      
        1 ttttcaccgg gttaaaaccc gttgttaata tctgtcgttc ctccggaact tttttcctat
       61 atctgcttta tgaatataga aataatattc accttttcta ctggtcgtta cgagtcctcc
      121 ttgccaaaca aatttgatta ttggttaaaa gttatttcgg cagtgacgac tcctgactta
      181 attctttctc aattctacat tcatagtaac agatattacc agtggtaatt tatttgaagt
      241 atcggggttt tatcttgcaa attaaaagat aaattagctt ttttgggtgg agctgtccac
      301 cttaaaacat caatggtact tactaaatga tttaagatta atatccctcc ggcaacaaga
      361 gcataattat cataatccat actccgatta cggattttgt tatattcact tctatgtttg
      421 gaactgtccc aataccagta tttatcattt ccataggcat tttcattaat atattgctgt
      481 tgactttccg gatcatcagg atataaattc atagctgttt gacgcaccca attattataa
      541 ccaccggcat caaaaccact actttcatag cgggctaatt gtttataaaa ttcatcacta
      601 taaccatccg gactcaaatg ggcaaattta atggcgtatt cataactttc ctgttccaag
      661 accttgcttt cgctatgaaa ataatagata gagccaatta taactgcttc tgcagtaagc
      721 attgcataac cgtaatttct gttagaactg atttgagacc atcctggaac ggcaaaagat
      781 tttaaagtta aaattcctct gctttgagca aatagcgatg tagaaagcag aataattaaa
      841 aggaaaatgc caaaacgctt cacagttatt ctcctaaaat tcaatttgat aattcagcat
      901 tgcaccgttt gcaggtaaag gagctaaata aaattctccg tgtttctttt tagataaaat
      961 ggaactatct ataatactga tacctctatt caataacatc agccccaaag caagctgatt
     1021 attcattttg gttttctgat gccgtttacg gagatgctta tattcttccc atttttcttc
     1081 gctttgccag ttccattctt cattgccttg ataagtattg gcggaaatgt attccgcaaa
     1141 ggattcaggg tcataattat aaattaaata atagttgcga gccatcattt cctgaatatc
     1201 attgtaataa tcgctattag gataattttg cactgcctga tagtgctcat caggcattcc
     1261 gtaaggcact ccggcataaa gttcggcaaa tttcatatag ttctttttct gcatctctat
     1321 ttcccgatca gtggcaataa aagcataaat atttattaaa tccacaccca ttaaagtagc
     1381 accccttgta ttatgcccta aaagcaattc acctgttccc ggtaaaatta aagaagaaac
     1441 aggatacaca aatttgggca aagctaataa gggaattatt ccccataaaa gcagaagaat
     1501 tataaagcag gttttccgtt tcaaaaagtc cacttcaacc ccaacatcgg tgttacattt
     1561 cccaaaggca tagcggcata gaaatttaag gaaggaactg attgagaaat gtaataacga
     1621 ttaaaattgc gggtaaccct aatggcatca agtcctgctg ctaaatgatt gaaagccaag
     1681 cccaaagtga aaaaatgtgc catactataa gagtccttag cgtcgttacg cattttaata
     1741 taggtctgac gcatcctgct catttcatgg gaatcaatag ggataagggt ttcagtatca
     1801 ttaccccata aaggataatt tccagtccag cgcatattgg gactatcatt ttctccatca
     1861 aatttccacc gaatggaatc acgcacacct gattcatcac aggcaaaacg ataataccaa
     1921 tccatccaac caaaaacata ttgagggtat ttacctatgt cttcataaaa atgttgagaa
     1981 tttgtcttat ctaagcgaaa atagccgtta tcataaatat catgttggtt tacacctatc
     2041 agaattgcct gaatttcatc ttgcctactt ctatcatacc ggaaacccgt tattttagtt
     2101 ccatctggag taatataggt tacaatttcg ccattggcat atttttcgta ttttttggtt
     2161 ttatctttgc cttggttatt gaaataaaca agtccgccaa ttagtccaat ttccagaact
     2221 gggaaaatat aggttgtaag aactgtctta ttggcataaa attgacctgc accaggaacc
     2281 agcatagaca tagccatagc taactgggca tttttccggt tgtagttttt tttaattaaa
     2341 tcgttagtta caacggaatc acgcattgca ttctgttgtt cttcttctgt ttgaattagc
     2401 cattggtcaa tatcggtttt gggtttttcc tgagagaaaa ggcaaaaggc aaaaccggca
     2461 ataaaaatta gcaggataaa tttcttcatt gctaaccccc taaaaaatca tttctc
//
LOCUS       DAOPFL010000166         2499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_58782_length_2499_cov_92.810556, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000166 DAOPFL010000000
VERSION     DAOPFL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58782_length_2499_cov_92.810556"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLG69_07545"
     CDS             <1..270
                     /locus_tag="PLG69_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPI43395.1"
                     /translation="QGKHLIRVCKGTACHVSDADGIKKAVMDALQLSEEENTTADMQF
                     TVMEVACLGCCSLSPVIMIDGRTYGKLTPGAIPAVLKQYEIEDTL"
     gene            267..2036
                     /locus_tag="PLG69_07550"
     CDS             267..2036
                     /locus_tag="PLG69_07550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPI43396.1"
                     /translation="MNNIIVKVGLASCGVAAGAKEVYTALEQYLENNPRPVILKKTAC
                     IGMCFEEPIVQFEGSELGSIHIGKANPETITHILEDYIAGKTPAENIILAEKISGKHN
                     ALLANQQRIVLRNCGIIDPECIEDYEAGDGYKALQKALQMERTAIIEEIKKSGLRGRG
                     GAGFPTGLKWQFCASENSDKKYIICNADEGDPGAFMDRSTLEGDPHSVIEGMIIGAYA
                     IGANEGYIYCRAEYPMALEHLKTAISAAEEKGYLGKNILGSSFSFDLHIKEGAGAFVC
                     GEETALMQSIEGKRGMPTLRPPYPAVKGLWGKPTNINNVETWANIPWIIMHGAQEFRK
                     YGTEKSPGTKVFALAGKIAGSGLIEVPMGIPLKDIIYKVGGGMKTSKPFKAVQMGGPS
                     GGCIPAEKLDTIVDYDSITATGAIMGSGGMVVMDSGTCMVDVARFFLNFTQNESCGKC
                     TFCRIGTKRMLEILTRITEGKGELQDIEKLEELAVNIIKGSLCGLGQTAPNPVLTTLR
                     YFKDEYLAHIVDKHCPAGVCNALITYKINPEKCIGCTLCARKCPVSCISGQVKQVHTI
                     DQSKCTKCGTCFQVCKFDAVTKE"
     gene            2048..>2499
                     /locus_tag="PLG69_07555"
     CDS             2048..>2499
                     /locus_tag="PLG69_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPI43397.1"
                     /translation="MINVSLNGKPVKVEPGITILELCKQNKIDIPTLCNDEELNPYGS
                     CWVCLVEVKGRKGFVTSCGTIISEGMEIITASESIHTARKMALELLISNHYADCIAPC
                     TIACPDFVDIQTYVSLIANGKYHEAVKVIKEKLPMPLSIGRICPAFCE"
BASE COUNT          794 a          424 c          567 g          714 t
ORIGIN      
        1 cagggaaaac atcttattcg tgtttgtaaa ggaactgcct gtcatgtttc cgatgcggat
       61 ggcataaaaa aagccgtaat ggatgcatta cagctttccg aagaagaaaa tacaactgcc
      121 gatatgcaat ttaccgtaat ggaagttgcc tgcctgggtt gttgttcttt atctccagta
      181 attatgatag acggaagaac ttatggtaaa ttaacacccg gggcaattcc tgctgttctg
      241 aaacaatatg aaatagagga tactttatga ataacatcat cgtgaaagtt ggtttagcaa
      301 gttgtggtgt tgcagcaggt gccaaagaag tatatactgc tctggaacaa tatcttgaaa
      361 ataatccgag acctgtaatt ctaaaaaaga cagcttgcat tggaatgtgt tttgaggagc
      421 caattgtgca atttgagggt tctgaacttg gttccattca tatcggtaaa gccaatccgg
      481 aaactataac tcatatactg gaggactata tagcaggaaa aacacctgct gaaaacataa
      541 ttttagcgga aaagattagt ggtaagcata atgctctttt ggcaaatcag caaagaatag
      601 ttttgcgcaa ttgcggaatt atagacccag aatgtattga ggattatgaa gccggagatg
      661 gctataaagc actgcaaaaa gcactgcaaa tggaaagaac agccatcatt gaagaaataa
      721 aaaaatccgg gttaagagga agaggcggag ccggttttcc tacagggtta aaatggcaat
      781 tttgtgcctc tgaaaattcg gataagaaat atattatttg taatgcggat gaaggagacc
      841 ccggtgcttt tatggaccgc agtactttgg aaggtgaccc ccattccgta attgaaggta
      901 tgattattgg tgcctatgct attggtgcta atgagggtta tatttactgc cgggcagaat
      961 atcctatggc tttggaacat ttgaaaactg caatttctgc tgctgaagaa aagggttatc
     1021 tggggaaaaa cattctgggt agctctttca gttttgattt gcatattaaa gaaggtgcag
     1081 gtgctttcgt ttgtggagaa gaaactgctt taatgcaatc cattgaagga aaaagaggta
     1141 tgcctacttt acgccctcct tatcctgcag ttaaaggact ttggggcaaa ccaacaaaca
     1201 tcaataatgt agagacctgg gcaaatattc cctggataat tatgcacggt gcacaggaat
     1261 ttaggaaata cggaacggaa aaatcacccg gaaccaaggt ctttgcctta gccggaaaaa
     1321 ttgctggtag cggtttaatt gaagttccga tgggaattcc cctgaaggat attatctata
     1381 aggttggagg aggaatgaag acctccaaac cctttaaagc agtccaaatg ggaggtcctt
     1441 ccgggggttg cattcctgct gaaaaacttg acaccatagt tgattacgat tctattactg
     1501 caacaggtgc aattatgggt tccggaggta tggtagtaat ggattcagga acctgtatgg
     1561 ttgatgtagc caggtttttc ctgaatttta cccaaaacga atcctgcgga aaatgcactt
     1621 tttgccgaat tggcacaaaa cggatgctgg aaattttaac ccgcattaca gaaggtaaag
     1681 gcgaattgca agacatagaa aaactggaag aactggcagt aaatattatc aagggctctc
     1741 tttgtggctt gggacaaact gctcctaatc ctgttttaac tactttacgc tattttaagg
     1801 atgaatattt ggctcatatt gtagataaac actgtcccgc aggtgtttgc aatgctctaa
     1861 taacttataa aatcaatccc gaaaaatgta tcggttgcac tctttgtgcc agaaaatgtc
     1921 ctgtttcctg tatctccgga caagtaaaac aagttcatac tatagaccaa agtaagtgta
     1981 ctaaatgtgg aacctgtttc caagtatgca agtttgatgc agtaactaaa gaataggagt
     2041 ttaggcaatg ataaatgtaa gtttaaatgg aaaaccagtg aaggtagagc ccggaattac
     2101 tattttggaa ctctgcaaac agaataagat agatattcct actttatgta acgatgaaga
     2161 attaaatcct tatggttcct gctgggtttg tttggtagaa gtgaaaggca gaaagggttt
     2221 tgtaacatct tgcggaacta ttatttcaga aggaatggaa attataaccg ctagcgagtc
     2281 aattcataca gcccgaaaaa tggctttgga attacttatt tccaatcact atgcggattg
     2341 tattgctcct tgcacaattg cttgtcccga ttttgttgat attcagacct atgtttcttt
     2401 gatagcaaac ggaaaatatc atgaggcagt gaaagttatt aaagagaaac tgccaatgcc
     2461 tctatctatt ggaaggatat gtcctgcctt ttgtgaaaa
//
LOCUS       DAOPFL010000167         2496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_58885_length_2496_cov_129.122900, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000167 DAOPFL010000000
VERSION     DAOPFL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58885_length_2496_cov_129.122900"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLG69_07560"
     CDS             <1..243
                     /locus_tag="PLG69_07560"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HPI43398.1"
                     /translation="DNITELVYSLEKNLHLKGFIIPGGNPVSAKLDICRTVARRAERK
                     IIALSRKENIAPELLSYVNRLSDFFFVLARKEENKT"
     gene            257..1006
                     /gene="gpmA"
                     /locus_tag="PLG69_07565"
     CDS             257..1006
                     /gene="gpmA"
                     /locus_tag="PLG69_07565"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPI43399.1"
                     /translation="MYKIVLLRHGESTWNKENLFTGWTDVDLSEKGIVEAHNAGKRLK
                     EEGFVFEETWTSVLKRAIRTLWIVLDEMDLMYIPVHHDWRLNERHYGALQGLNKAETA
                     AKYGEEQVLLWRRSYDTPPPPLEKSDPRYPGFDPRYANLREDQLPLCESLKDTVKRTM
                     PFWNEIIMPRLAAGRKMIVSAHGNSLRAIVKSLSKISDADIVGLNIPTGIPLIYEFDD
                     NLQPVKNYYLADKAELESAQQAVANQGKAKT"
     gene            complement(1011..1463)
                     /gene="purE"
                     /locus_tag="PLG69_07570"
     CDS             complement(1011..1463)
                     /gene="purE"
                     /locus_tag="PLG69_07570"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPI43400.1"
                     /translation="MHPQVRIIIGSKSDLEKITPAREILEEFEVSYDLHISSAHRNPE
                     KTIALAQNAKAEGIEIIIAGAGLSAHLPGFIAAHTTLPVIGIPLASGALNGLDALFSI
                     VQMPSGVPVACMAINGAKNAALYAVQILALKDKDLEKKFSAYKKGLEG"
     gene            complement(1467..2135)
                     /locus_tag="PLG69_07575"
     CDS             complement(1467..2135)
                     /locus_tag="PLG69_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPI43401.1"
                     /translation="MAKKFISKAEAAKKLKVSERVIQEMIATNVFESKLIGKNVKIDE
                     DSLNEWLENLNETEEKMLALKRVICHFEEYMRPENIFLDFTAENKYDAIRILSEKAKE
                     LKLVRDARWLYEVVVAREELVSTAIGNGVALLHPRHLHPSKIKAPSILFGRSSEPVDF
                     DAPDNKPVNIFFMLLLHNDKQHLFSLSYISRLVMNQDILKGLTEAKDAKEIHQLLTVI
                     PEQT"
     gene            2231..>2496
                     /gene="lptE"
                     /locus_tag="PLG69_07580"
     CDS             2231..>2496
                     /gene="lptE"
                     /locus_tag="PLG69_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPI43402.1"
                     /translation="MVKKFIYTILLLVVLSSCHYSVYSNAYPHLRKIRILAFENRTSE
                     YGLGDKLLNYLNREIREDGRLKLVTEDPDCTLEGAILSFSENVY"
BASE COUNT          774 a          458 c          511 g          753 t
ORIGIN      
        1 gataatatta cggagcttgt ttattctctg gaaaaaaacc tgcacttaaa gggttttatt
       61 attcccggcg gaaatcctgt ttctgccaag cttgatatct gcagaaccgt tgccagaaga
      121 gccgaaagaa aaataatagc cctttcccgt aaggaaaata ttgctccaga attgcttagc
      181 tatgtaaacc gactctcgga tttctttttt gtcctggcaa gaaaagagga aaacaaaact
      241 taaaaaggag ttagttatgt ataaaatagt attactgcgt cacggtgaaa gcacttggaa
      301 caaagaaaat ctgttcacgg gctggacaga tgtagacctt tccgaaaagg gtatagttga
      361 agcacataat gcaggtaaac gactgaagga agaaggtttt gtttttgaag aaacctggac
      421 ttccgtattg aaaagagcta tcaggacttt gtggatcgtt ttggatgaaa tggatttgat
      481 gtatattcct gttcatcatg attggcgact taatgaacgt cactatggcg ctttgcaagg
      541 tttgaacaaa gctgaaacag ctgctaaata tggagaagaa caagttttac tttggcgtag
      601 aagttatgat acacctccac cacctttgga aaaaagtgat cctcgttatc cgggatttga
      661 tccccgatat gcaaatttaa gggaagatca acttcctctt tgtgaatcgc tgaaagatac
      721 cgttaaaaga acaatgcctt tctggaatga aataattatg cctcgtttag ctgcaggcag
      781 aaaaatgatt gtttctgcac acggtaatag tttacgcgct atagtaaaaa gtttaagtaa
      841 aatcagcgat gctgatattg ttggtcttaa tattcctacc ggcataccac taatctacga
      901 atttgatgat aatttacaac cggtaaaaaa ttattatctg gcagataaag cagaactgga
      961 atctgctcaa caggcagttg ccaatcaggg taaggcaaaa acctgaatct ttagccctcc
     1021 agtccttttt tataggcaga aaatttcttt tccaggtctt tatctttcag agctaatatc
     1081 tggacagcat acagagctgc atttttagct ccgtttattg ccatacaggc aacgggaact
     1141 cctgaaggca tttggacaat ggaaaagaga gcatccaatc catttaaggc acctgaggca
     1201 agaggaatac caattacagg caaagttgta tgagcggcaa taaatcctgg taaatgagca
     1261 gataaaccgg ctccggcaat aattatttct attccttccg ctttggcatt ttgagcaagg
     1321 gcaattgttt tttcaggatt gcggtgagca gaagaaatat gcaggtcgta agagacctca
     1381 aactcctcca gtatttccct ggcaggagta attttctcca aatcgctttt actgcctata
     1441 ataatcctga cttggggatg cattgtttaa gtttgttcgg gaattacagt aagaagttgg
     1501 tgaatttctt tggcatcctt tgcttccgtt aaacccttta agatatcctg attcataact
     1561 aagcgggaaa tgtaagaaag cgagaaaagg tgctgtttat cattatgcaa aagaagcata
     1621 aagaaaatat tcaccggttt gttatcagga gcatcaaaat ctacaggttc agaagaacga
     1681 ccaaacaaaa tgctgggggc tttaatttta gaaggatgca aatgacgcgg atgaagcaag
     1741 gcaactccat taccaatggc tgtagaaacc aattcttcac gagcaactac aacttcataa
     1801 agccagcgtg catcgcgcac aagtttcagc tctttcgcct tttcacttaa gattcggata
     1861 gcatcatatt tgttttctgc cgtaaaatcc aggaaaatgt tttcgggacg catatattct
     1921 tcaaaatgac agataacccg ctttaaagcg agcatttttt cttctgtctc attcaaattt
     1981 tccagccact cgtttaagga atcttcgtct attttaacat tttttcctat gagcttactt
     2041 tcaaaaacat tcgtagcgat catttcctga atgacgcgct ccgaaacctt taacttcttg
     2101 gcagcttcag ctttagagat aaattttttc gccataaaag aactcctttt ttaggtttta
     2161 ttattatttt atatctgctt tggcaaggtt tagcttgaca ataatataag cattttttga
     2221 agatggaata atggtcaaga aatttattta tacgattctg cttttggttg tcttaagcag
     2281 ttgtcattat tcggtttatt ccaatgctta cccgcatttg cggaaaataa gaattctggc
     2341 ttttgaaaat agaaccagcg aatacgggtt gggggataaa ctgcttaatt atttgaaccg
     2401 ggaaatcagg gaagacggaa ggttgaaact ggtgacagaa gaccccgatt gcactctgga
     2461 aggagctatt ttaagttttt ccgaaaatgt ttacag
//
LOCUS       DAOPFL010000168         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_59258_length_2485_cov_106.410700, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000168 DAOPFL010000000
VERSION     DAOPFL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59258_length_2485_cov_106.410700"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2430
                     /locus_tag="PLG69_07585"
     CDS             <1..2430
                     /locus_tag="PLG69_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPI43403.1"
                     /translation="NMPTKVWIGTTTLTSLSDAWIPVSELTLVFDGNINYPIGDNIIT
                     IPFNAPYFYLNGENLVIFVQRPWEDVYHNSSDDFHAQTDATYTGRSRRMYSDSVTYDP
                     NNLPATGATVSGQFPKTTLIVIPGGVGHLEGTVVATGSIPLEGVAVNIATTTYGAVTD
                     AQGHYHITNIIPDDYTVNFSKYGYISQSINITIEEDETEILNVTMQPMPTVSVTGTIV
                     ASDTGAGINGASIYLVGYQNYNTTSSATGAFSFPAVYANQTYEYTIIAPGYTTVTGTI
                     NVGATNYNMGSITMNEVAYAPFGVVAALNDIYTAVNISWQAPDPTAVDVTEGFEGTVF
                     PPLQWTQIINNTGPEIIPGVLPTWCRFGTVNPGGNPVAPPQGNYQAGLWWDYEHQDEW
                     LITPTFNCPPMAYLTVDAYVYRGSIYNDHYYIKVSTDNGTNWTILYDATAATGGWNYY
                     VSPIYLNMEAYGGQQIKIAFQADDPPSNDGLWYCWFIDNLYIGNAVQGVKFAGSDLLS
                     SRSKVAGEKVSSEIVVAPYPSREAIEGKRELPTVLKDKITTPTRSERSLQGYKVWRLT
                     SGNETQPDTWTLLTTQLITTLATVDSAWTALPNGSYRWAVKAVYTANVTSVASFSNVL
                     NKETQMGNIVGFVRKQNNQPIAGATVTAGGISATTNSAGAYSLALSIGTYSVTASAAG
                     YQPRTIDGVVVLPNQNTTLNFVLDPVANEDEYLPAVSTELIGNYPNPFNPETTIAYSI
                     KDRCKVRLEVYNLKGQLVRTLVNEEKANGHYKAVFNAKDEKGNTLSSGIYFYRLQAGN
                     YVSTQKMLLME"
BASE COUNT          683 a          512 c          556 g          734 t
ORIGIN      
        1 aatatgccta ctaaggtctg gatcggaact accactttaa cttctttgtc ggatgcctgg
       61 attccagttt cagagttaac ccttgttttt gacgggaata taaactatcc cattggcgat
      121 aatataatta ctattccctt caatgctccc tatttctatc tgaatggaga aaacctggta
      181 atctttgtgc agcgtccctg ggaagatgta tatcacaatt ccagcgatga tttccacgca
      241 caaacggatg ccacttatac agggcgctcc agacgaatgt atagtgattc tgttacttat
      301 gatcccaata atttgcctgc tacaggagca actgtttccg gtcaattccc caagaccaca
      361 ctaattgtta ttcctggagg agtgggacat ttggagggca cggtagttgc tactggcagt
      421 attcctttgg aaggtgtagc ggttaatatt gccactacca cttacggcgc agttacagac
      481 gctcagggac attatcatat taccaatatt attcctgatg attatactgt gaacttcagc
      541 aagtatggtt acatcagcca aagtatcaat attaccatag aggaggatga gaccgaaatt
      601 ctgaatgtaa caatgcaacc gatgcctacc gtttctgtta ccggaactat tgtagccagt
      661 gatacaggag ctggaattaa cggtgcttcc atctatctgg ttggttacca aaattataat
      721 acaactagtt ctgctactgg tgctttcagt tttcctgcgg tttatgccaa tcagacctat
      781 gaatatacaa ttattgcccc cggttataca actgttacgg gaacaatcaa tgtaggagcc
      841 actaactaca atatgggcag tatcactatg aacgaagttg cttatgcacc ttttggtgtt
      901 gtagcagctc tgaacgatat ttatacggca gtgaatatca gttggcaggc acctgatcct
      961 acagcggtag atgtaactga gggttttgaa ggaactgtct ttccgccttt gcaatggact
     1021 caaatcatca ataatacagg accggaaatt attcctgggg ttttaccaac ctggtgcaga
     1081 tttggaactg tgaatcctgg aggaaatcct gttgctccac cgcaaggaaa ttatcaagca
     1141 ggtctgtggt gggattatga acatcaggat gaatggttga ttactcctac tttcaattgt
     1201 cctccaatgg cttatcttac tgtagatgcc tatgtttatc ggggttctat ttataacgac
     1261 cactattaca tcaaggtttc taccgataat ggcacaaatt ggactatttt gtatgatgct
     1321 acagccgcta caggtggctg gaattattat gtctcaccta tctatctgaa tatggaggca
     1381 tacgggggac agcagattaa aattgctttt caagccgatg atcccccttc caatgatggc
     1441 ctctggtatt gttggtttat agataatctc tacattggca atgcggttca aggtgtgaaa
     1501 tttgcgggtt ccgacctgct ttcttctcgt tctaaagttg cgggagagaa ggtctcttcc
     1561 gagatagttg ttgctcccta tccttcccga gaagcaattg aaggaaagag agagcttccc
     1621 acagttttga aagacaaaat tacaactcca acgcggtcgg aaaggtcttt gcagggctat
     1681 aaggtctggc gtttaactag cggaaatgag acccaaccgg atacctggac tctgctaacc
     1741 actcaattga taactacttt ggcaacagtt gattctgcct ggactgcact tcctaatgga
     1801 agttaccgtt gggctgtgaa agctgtttat actgccaatg ttacttctgt tgcttcattc
     1861 tccaatgttc tgaataaaga aacacagatg ggtaatattg ttggttttgt gcggaaacaa
     1921 aataatcagc ccattgccgg agccacagta actgccgggg gaatttctgc taccactaat
     1981 tcagcgggag cttattcttt ggctttatcc ataggaactt acagtgtaac tgcttcagca
     2041 gcgggttatc aaccgcgaac aattgacggg gttgtggttt tgcccaatca aaataccacc
     2101 ttgaactttg tgcttgatcc ggtagctaat gaggatgaat atttgcctgc tgtatctacg
     2161 gaactgatag gtaactatcc caatcccttc aatccggaga ccactattgc ctattctatt
     2221 aaggatagat gcaaggtgcg tttggaggtc tataacctga aagggcaact ggtgagaacc
     2281 ttagtgaatg aagaaaaggc aaatggacat tataaagcgg tctttaacgc taaggatgag
     2341 aaaggtaata ccctttcttc cggtatctat ttctatcgct tgcaggcggg aaattatgtt
     2401 tctacgcaga agatgctgct gatggaatag gcagagttga agggttagac actaataatt
     2461 atatagcaag ggcggaagtt aattc
//
LOCUS       DAOPFL010000169         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_60131_length_2459_cov_99.647671, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000169 DAOPFL010000000
VERSION     DAOPFL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60131_length_2459_cov_99.647671"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLG69_07590"
     CDS             complement(<1..774)
                     /locus_tag="PLG69_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPI43404.1"
                     /translation="MKQIFPVSAGYYWSDCGAFMGVLPYPLWQTKTILDQRQRRRLNL
                     NLLLIQSGKRNILIDTGIGNRLSDKQRDIYQPSEFLLPFSLAELGLKDRDITDVIMTH
                     LHFDHAGGIITNFGTYDALTFPKAHYWIQKAEWEIAKNPDELNRSAYNFEQQLALLEK
                     QGNITIIDGEAEIEPGILLTLVGGHTIGSQIVTIDFGTDYYIYAGDIIPTLFHTSLAI
                     TSAYDVSRIQTVTAKKLIYNRLKEHNGTLILNHDTTQWQI"
     gene            complement(771..1574)
                     /locus_tag="PLG69_07595"
     CDS             complement(771..1574)
                     /locus_tag="PLG69_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HPI43405.1"
                     /translation="MTTIDKSGESLEEIIRTFREDYKIHDWELNYEILKKPTKGIFGL
                     FAHKLALVRFQLPSSFDRAELFMQNLLQKMGISYTSLKSRTEGKTLFLEINGSPDSGF
                     LIGKNGSMLDTLQYLINRVFEDNAQIERIYLDVDGYRERQADSFLKPYLPQILQVKIT
                     GKPITLEPMPAAERRIIHRYVEQEKGLRTLTIGEGEKKRIVVFSAEQNQKEALSQSRT
                     EPKRRNNKAPIHTHKPVPTPNNKTGNTSLKVKKPNRQQKQPKVSNTKHQ"
     gene            complement(1571..>2459)
                     /gene="yidC"
                     /locus_tag="PLG69_07600"
     CDS             complement(1571..>2459)
                     /gene="yidC"
                     /locus_tag="PLG69_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPI43406.1"
                     /translation="TLVLKENEPALMRSFRTELNPQTGNPAFVIDSKQSNPKQSWSQS
                     FIIYAGPTDYQILQKYGKQMDNIPERGASWLRWLSNIFEWILKFLHRYIKNYGVVILI
                     FAIIIKLLLHPLTQHQMVHSMRQQKMQPYIEEIRKKYANDVVKQREELTKLYKENKTS
                     MTAGCLPLLIQIPILIPLYSVLRYSLDMRNASFCLWLKDLSEPDPYMILPIIMGGFML
                     LQSLMMRPKNVDTSQMTEQQKMQQSQMKIMTWGMPIFMVIIFRGMPAGLVLYWTAFNV
                     LSILHQYFLTKHLKKKENK"
BASE COUNT          697 a          449 c          491 g          822 t
ORIGIN      
        1 tatctgccat tgtgttgtat catgatttaa aataagtgtt ccattatgtt cttttaatcg
       61 gttgtaaatt agttttttag cggtaactgt ctggatgcgg gaaacatcat aagcggaagt
      121 gattgccagg gaagtatgaa acaaggtagg tataatatct cctgcataga tataatagtc
      181 agtgccgaaa tcaattgtaa caatttgact gccaatagta tgtccgccaa caagagtaag
      241 caagattccg ggttcaattt ctgcttcacc atcaattata gttatatttc cctgtttttc
      301 cagcaaagcc aattgctgtt caaagttgta agctgagcga tttagttcat caggattttt
      361 tgctatttcc cattccgctt tttgaatcca atagtgtgct ttaggaaaag tgagggcatc
      421 ataagttcca aagttagtaa tgattccacc tgcatggtca aaatgcagat gagtcataat
      481 cacatcggta atgtctctat ccttaagtcc caattccgca agagagaaag gcaggagaaa
      541 ttcgctcggc tggtaaatat ctctttgttt atcggaaagt cggtttccga ttcctgtatc
      601 tattaaaata ttacgcttgc cactttgaat aagaagcagg tttaaattca gtcggcgtct
      661 ttgtctctga tccagaatgg tctttgtttg ccataaagga tagggaagaa cacccataaa
      721 agcaccgcaa tcagaccaat agtaaccagc cgagacaggg aaaatttgtt tcattgatgt
      781 ttggtgttgc ttacttttgg ttgtttttgt tggcgattgg gttttttcac ttttaaggag
      841 gtatttccgg ttttattgtt tggtgtggga acgggtttat gagtatggat tggggctttg
      901 ttgtttctcc ttttgggttc tgttctgctt tgggaaagtg cttctttttg attttgttcg
      961 gcagaaaaaa caactatgcg tttcttttct ccttcaccga tagtgagggt gcgtaagccc
     1021 ttttcctgtt ccacatagcg atgaataatt cgtctttcag cagcaggcat tggttccaga
     1081 gttataggtt tgcctgttat tttaacttgc agaatttgag gaagataggg ctttaaaaaa
     1141 ctgtctgcct gacgttccct ataaccatca acatccaaat aaattctctc aatttgagca
     1201 ttgtcttcaa acacacgatt gattaaatat tgcaaagtat ccagcataga accatttttc
     1261 ccaattaaaa atccggaatc gggactaccg ttgatttcca aaaagagggt tttgccttcg
     1321 gttctgcttt ttaaagaagt gtagctaatt cccatttttt gcaataagtt ctgcataaaa
     1381 agttcagctc tgtcaaaaga tgaaggcagt tgaaaacgca ccagtgcaag tttatgggca
     1441 aataagccaa atataccttt agtgggcttt ttcaggattt cgtaatttag ttcccaatcg
     1501 tgaattttat agtcctcgcg gaaagtcctt attatttctt ccagggattc tccgctttta
     1561 tctatagtag tcatttattt tcctttttct tgaggtgttt ggttaaaaaa tactgatgaa
     1621 gaatagagag cacattaaaa gctgtccaat ataaaacaag tcctgctggc atcccgcgaa
     1681 agataatcac cataaaaata ggcattcccc aagtcattat tttcatttgg gactgctgca
     1741 ttttttgttg ttccgtcatt tgcgaggtat ccacattttt aggcctcatc attaacgact
     1801 gtagcagcat aaaaccgccc ataataatcg gcagaatcat ataaggatcg ggttcagaaa
     1861 ggtctttcag ccataaacaa aaggaggcat tacgcatatc caaagaataa cgtaaaacac
     1921 tgtaaagggg aataagaatg ggtatttgaa tcagcaaagg caggcagccg gcagtcatac
     1981 tggttttgtt ttccttatag agtttcgtaa gctcttcccg ttgtttcact acatcattag
     2041 catatttttt tctgatttcc tctatatagg gttgcatttt ttgctgacgc atactgtgca
     2101 ccatttgatg ttgcgttaaa ggatgcagca aaagctttat aataatggca aaaataagaa
     2161 ttaccacccc ataattttta atgtagcgat gcaggaattt aagaatccat tcaaaaatat
     2221 tggagagcca acgtagccag cttgctcctc tttcaggaat gttatccatc tgttttccat
     2281 atttctgcaa aatttgatag tccgttggtc ccgcataaat tataaaactt tgactccaac
     2341 tttgtttggg attgctttgt ttagaatcaa taacaaacgc cggattaccg gtttgaggat
     2401 tcaattctgt tctaaaggaa cgcattaaag cgggctcatt ttcttttaag acgagagta
//
LOCUS       DAOPFL010000170         2444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_60636_length_2444_cov_92.439514, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000170 DAOPFL010000000
VERSION     DAOPFL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60636_length_2444_cov_92.439514"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1983)
                     /locus_tag="PLG69_07605"
     CDS             complement(10..1983)
                     /locus_tag="PLG69_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPI43407.1"
                     /translation="MRKLVFLAFLFSTCLNATVITIGSENQLNKGLPIEPIAHFSYSQ
                     QIYLPSEIGCTGIITTLSFQYNVISNVFYNSNKSWKIWLGHTPIAEITNWIPLNDMQV
                     VYDNNLQESDFLGGLPGNGWLVIELETPFLYNGMDALLVAVDENSIGYGNNSDDFSCS
                     SCPLDYAIQYQSSQINPDPLAPPESGWNLKNFRSNLQITIEAQYYTPVQPIPANASNN
                     IPIDTTLSWTSICNSFSLVLGTHSDSLLQVVDQTPLTYWQPETPFQFGKTYYWQVTGY
                     ANNCEYVSPLWSFTTITEQISPPQNLTGCYNGSASQLNWNAPVTGNQHYYKIYRNASF
                     YITTLETSYYDYNVQQGLSYYYYVTAVSFSGSESTPSNVITITIPAGGTDRILFQGFE
                     TCPSFTGIIANWQNLDLDLAPTSFWQNVTYPNAGNPAGWLCFEPQSTIPPLTDHPAFE
                     GNKMLASICCIPPPNNDWLISPVIHLGTASSLQFKARSAYANFGLERLSVLISNINSE
                     PASFVPLHQNTYIAVPVEWTNYEYDLSAWNNQNVYLAWRCLSVDAMALFLDNIEVFSH
                     NGYVSNQDIELPKPLFCCYPNPSKDSFTLESKDGTAFDVELYNLKGQCLYKEKGIKKL
                     QSSKLNCNLANGIYFMRIAQAGKIVTLKQVVIK"
     gene            complement(2122..>2444)
                     /locus_tag="PLG69_07610"
     CDS             complement(2122..>2444)
                     /locus_tag="PLG69_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HPI43408.1"
                     /translation="KVHCSVLAADTLQSAISNYEIAHGLKEVPNFGKATIEEELRKII
                     YPQIGEDIISLKMVKYIGFQDGEVTIDMNIMKFDQWRDNIAEEIREHLQKYPEVKKIT
                     INFP"
BASE COUNT          755 a          447 c          464 g          778 t
ORIGIN      
        1 ggagaaagat tattttatta caacctgctt gagagtaact atctttcctg cttgagcgat
       61 ccgcataaaa taaatgccat tagctaaatt gcaattcagt ttagagcttt gcagtttttt
      121 gatgcccttt tctttataca ggcattgtcc cttcaggtta taaagttcca catcaaaggc
      181 agttccatct ttgctctcta atgtaaaaga gtcctttgag ggattgggat aacagcaaaa
      241 aaggggtttg ggaagttcta tatcctgatt ggaaacatag ccattatgac taaagacctc
      301 aatgttatcc aaaaacaaag ccattgcatc tacggaaaga caacgccagg caagataaac
      361 attttgatta ttccatgcag ataaatcgta ttcgtaattt gtccactcaa caggaacagc
      421 gatataggta ttttggtgta aaggtacaaa agaagcaggt tcgctattga tattggaaat
      481 tagaacactt aagcgttcca atccaaaatt ggcataagca gaccgggctt taaattgtag
      541 ggaagaagct gttcccaaat gaataacggg cgaaatcaac caatcgttat taggaggtgg
      601 aatacaacaa attgaagcaa gcattttatt accttcaaaa gccggatgat ccgttaaagg
      661 aggaattgta ctttggggtt caaaacaaag ccaaccagct ggatttcctg cattggggta
      721 agttacattt tgccaaaagg aagttggagc caaatccagg tccagatttt gccagtttgc
      781 tattattcca gtaaaagaag gacaggtctc aaagccctgg aataatattc tgtctgttcc
      841 tccagctgga atagtgattg ttattacatt ggaaggagtg ctttccgagc ccgaaaaact
      901 tactgccgtt acatagtaat aataacttaa gccctgttgc acattgtaat cgtaataact
      961 tgtttcaagg gttgttatgt aaaaactggc attccggtat attttgtaat aatgttgatt
     1021 tcctgttaca ggtgcgttcc aatttaattg agaggcagaa ccattatagc aaccggttaa
     1081 attctgtggg ggagatattt gttccgtaat agtagtaaaa ctccagaggg gagaaacata
     1141 ttcacaattg ttggcataac cggttacctg ccaataatag gtcttaccga attgaaaagg
     1201 tgtttccggt tgccaatagg ttaaaggtgt ctgatctacc acttgcagta aggaatccga
     1261 atgcgttccc aaaacaagag aaaagctgtt acagatgctt gtccaggata aagttgtatc
     1321 tatgggaata ttgtttgatg catttgcagg aattggctga acgggagtgt aatattgtgc
     1381 ttcaattgtg atttggaggt tactgcgaaa gtttttcaaa ttccagccgc tttctggagg
     1441 agctaatgga tcaggattga tttgggaact ttgatattga atagcataat ccaagggaca
     1501 ggaactacaa ctaaaatcat ctgaattatt gccatagcca atgctgtttt catcaacggc
     1561 tactaataaa gcatccattc cattgtataa aaaaggtgtt tctaattcaa taaccaacca
     1621 tccgtttcct ggtaatccac ctaagaaatc cgattcttga agattattat cataaacaac
     1681 ttgcatatcg ttaagcggaa tccagtttgt tatttcagct atgggagtat gtcccagcca
     1741 tattttccag ctcttattgg aattataaaa cacattactg atgacattgt attgaaagct
     1801 aagggtagtt attataccgg tacaacctat ttctgaaggc agataaattt gctgtgaata
     1861 gctgaaatga gcaatcggct caataggtaa acctttattc agttgattct cacttccgat
     1921 agtaataacg gttgcattta aacaagtaga aaacaaaaaa gctaaaaata caagtttacg
     1981 catatcatct ttccttcata agttattgga ttttaacctg tactatcatt ctctattctt
     2041 tctatcagaa aactcactat tttattcctg cgttttacac tttcactttt ccccttaacc
     2101 tgttaacctg gttacctttt tctacgggaa gtttatcgta attttcttca cttcggggta
     2161 tttttgcaag tgttcacgga tttcttccgc aatattatcc cgccattgat caaatttcat
     2221 tatgttcata tcaatagtca cttctccatc ctgaaagcca atatacttca ccatttttag
     2281 agagataata tcttctccta tttgcggata gataattttt ctcagttcct cttctatagt
     2341 tgctttaccg aaattgggaa cttctttcaa gccatgtgct atttcgtaat tggaaattgc
     2401 cgattgtaaa gtatcagcag ctaaaacact gcagtgaact ttaa
//
LOCUS       DAOPFL010000171         2415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_61666_length_2415_cov_91.905932, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000171 DAOPFL010000000
VERSION     DAOPFL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61666_length_2415_cov_91.905932"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(453..1547)
                     /locus_tag="PLG69_07615"
     CDS             complement(453..1547)
                     /locus_tag="PLG69_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HPI43409.1"
                     /translation="MKPFKPEKLPLSCIDWERQISFISQAHNLLGKYEGMLQTIVNPN
                     LFLSPLTTQEAVLSSKIEGTQATLDEVLRYEANPKMVLSPERNADIQEILNYREAINY
                     AVERLKSIPLSLNLIKDVHSILMNSVRGMNKTPGEFRKVQNYIGPPGADISQATYIPP
                     SPEEVMPAMDNLEKYIHYEEKDTIVQLAIIKAQFEIIHPFLDGNGRIGRILIPLFLFY
                     KNIISKPVFYLSTYLEKHRDEYYQHLNNITAKNDWNGWISFFLKAVIEQAKMNIEKAG
                     AIQNLYDQMKITIPEITGSKYSMQLTDALFISPLFSINGLSTASMIPKATIIRIVNIL
                     KNEKIVTEIQKSRGQMPGLFKFKALYNIVK"
     gene            complement(1874..>2415)
                     /locus_tag="PLG69_07620"
     CDS             complement(1874..>2415)
                     /locus_tag="PLG69_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPI43410.1"
                     /translation="LQERLAKLSSGVAVLRIGAATETEMKEKKARVDDALHATRAAVE
                     EGIVPGGGITLIQAAKALKQLKGLSYEEKMAVDILSKALEKPAYQIAANAGEEGAVVV
                     EKLKSFRDIHMGFNASNGQFEDLFKAGIIDPAKVVRSAIQNAASIAGLMLTTECIITD
                     IKEPEPAVPMPNPNMADMY"
BASE COUNT          675 a          451 c          428 g          861 t
ORIGIN      
        1 tgacattttt tcctcctgtt tatttttaat cctcatatac tgaataaggt ttttttggca
       61 aggaaaattt gggaagacga gatagaaagt agaaaggtct taaggtcttg aggtgcaaag
      121 atcttaaggt ctttatttct ttacttctca accctctaaa ccttctaaac tctctaaact
      181 ttctcaaccc tctaaaaact ttctcaaccc tctaaaccct ttatccgtca tttacgatgt
      241 taaatatgtg tttcactttt tcacttttcc ccttcacctg ttaacctgtt atcctattcc
      301 accttttctg ctattttatc ataacaactt gagagagaat gaaatacagc gcttgtgttt
      361 catagaatat agacaaggga ttttgtgttg cataaaacac taaagatgag gcacaaacac
      421 tctatattat ttatttattg ttagttatac atttatttaa caatgttata aagagctttg
      481 aacttaaaca atcctggcat ttgtcctctg ctcttttgta tttctgtcac gattttctca
      541 ttttttaata tatttactat tcttatgatg gttgctttag gaatcattga agctgtggaa
      601 agaccgttaa tactgaaaag aggtgatata aaaagcgcat cagtcagttg cattgaatat
      661 ttagaaccgg taatttcagg aattgtaatt ttcatttgat catacaaatt ttggatagct
      721 cctgcttttt caatattcat ttttgcctgt tcaataactg ctttcaggaa aaaagaaatc
      781 catccgttcc agtcattttt tgcagtaata ttatttaggt gctgataata ttcatctcta
      841 tgtttttcca ggtaagtgct taagtaaaat accggcttgc ttatgatatt tttatagaac
      901 agaaagagag ggatgagaat tcttcctatt cttccatttc catccaggaa agggtggatt
      961 atttcaaatt gtgctttgat tattgccaat tgaacgattg tatctttttc ttcataatgg
     1021 atgtattttt ccagattatc catagcaggc atcacctctt cgggagaggg agggatatag
     1081 gttgcctggg aaatatctgc acctggagga ccaatgtaat tttgaacttt tctaaattct
     1141 cccggagttt tattcattcc tctaacgctg ttcatcaaaa tggaatggac atctttgatc
     1201 aaatttaagg atagaggtat ggattttaac cgctcaactg cataattaat tgcttcccga
     1261 taatttaata tctcttgaat atcagcattt ctttcaggag atagtaccat tttaggattt
     1321 gcttcatagc gcaagacctc atccaaggtt gcctgtgtgc cttcaatttt agaagataaa
     1381 actgcttcct gagtagtgag aggcgataaa aacagattgg gatttactat tgtttgtaac
     1441 atcccttcat atttacctaa caaattatgg gcttggctaa taaaacttat ctgtctttcc
     1501 caatcaatac aggaaagagg aagcttttct ggcttaaaag gtttcatctt gtttttccca
     1561 tacttaaaat tctataattg ctttcttatt taatgataag ttattgtcaa tgtttttatt
     1621 agttgagagg tctgaaggtc tggaggagga aaagtcataa gtcgtcactg cctttaataa
     1681 ttttacacat ctcacgaatt tatttggcag tgaggactca taacgattta gcacgcagat
     1741 ttcgcgaatt ttattataat aattttgcct tttctttttc tagcccgtgt ttcactttcc
     1801 acttcaaccg ttcatccaaa aagggctttt ccttttcgga aaagcccttt tctttatttt
     1861 agacgggatt atattaatac atatcagcca tattaggatt gggcataggg acagctggct
     1921 caggttcttt aatatcggta ataatgcatt ccgtagttaa catcaaaccg gcaatggaag
     1981 cagcgttttg gatagctgag cgaacaacct tagcggggtc aataattccg gctttgaaga
     2041 ggtcttcaaa ttgaccattg ctggcattga atcccatatg gatatcgcga aagctcttta
     2101 gtttttcaac aactacagcg ccttcttcac ctgcgttagc ggcaatttga taagctggtt
     2161 tttccagtgc tttagacaaa atatccacag ccattttttc ttcgtaggaa aggcctttta
     2221 gttgttttag ggctttagcg gcttgaatta aagtaattcc accaccggga acaatacctt
     2281 cttcaacagc agcccgggta gcatggagag catcatctac gcgagctttc ttttccttca
     2341 tttctgtctc ggtagcagca ccgattctga ggacggcaac tccgctggaa agtttggcaa
     2401 ggcgttcctg caatt
//
LOCUS       DAOPFL010000172         2404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_62079_length_2404_cov_104.129842, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000172 DAOPFL010000000
VERSION     DAOPFL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62079_length_2404_cov_104.129842"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="PLG69_07625"
     CDS             complement(<1..1511)
                     /locus_tag="PLG69_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPI43411.1"
                     /translation="MYKTIDLKESPRHLENRIREYWNKHDMVQKSIDIREGNPRFIFY
                     EGPPTANGKPGIHHIMARTLKDVICRYKTMNGFQVKRKAGWDTHGLPVEIEVEKMLGL
                     KDKRGIEEYGIEKFCQKCRESVFSYEKLWREMTELMGYWIDLDHPYVTLHNSYIESVW
                     WILHNFFSRNLIYKAYKIVPYCPSCGTPLSSHEVAQGYKDIEDPSIYVKFKATDEENT
                     YYLAWTTTPWTLISNVALAVHPEETYLKVEYQGQNLILAKARLQVLDEETKIISEMQG
                     RELEGRCYQPLFNFVSVDKPAWYICLGDYVTMSEGTGIVHTAPAFGQDDYSLALKYDL
                     PFVQPVDSEGKFTEAVTPWAHQFVKDADKDIIRHLKERGNLLKREQIKHSYPFCWRCK
                     SPLIYYARESWYIKTTAYKEQLIENNRKIIWYPPFVGEKRFGDWLENNVDWALSRDRF
                     WGTPLNIWICEKCGKLRSLGSIEELRKEGKMTSGKDVPENLDLHRPYIDEVVLT"
     gene            complement(1517..1882)
                     /locus_tag="PLG69_07630"
     CDS             complement(1517..1882)
                     /locus_tag="PLG69_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43412.1"
                     /translation="MFILDEIHLDVAKAIAEKNRKKKSCDHCYDRGWIGVSEQNLLVL
                     CPKCVDREKAIEEWKEYVSEHKELKEHFSELFEEKKVESEQENPLKQKEFERTKNHPA
                     NPHNFVPGQRRSGHQKKIG"
     gene            complement(2171..>2404)
                     /locus_tag="PLG69_07635"
     CDS             complement(2171..>2404)
                     /locus_tag="PLG69_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43413.1"
                     /translation="EIEALSAEDKLALSRNKNLFIVTHRLEPIVKIELKKTVISAPIQ
                     FMESAPKEEEPIEEVTPLPINPEKSKPKRTPKN"
BASE COUNT          682 a          515 c          408 g          799 t
ORIGIN      
        1 gtaagaacaa cttcatcaat atagggacgg tgtaaatcaa ggttttccgg aacatctttt
       61 ccggaggtca ttttgccttc tttgcgcaat tcttcaatag aaccaagaga acgcagtttt
      121 ccacattttt cacaaatcca gatattgagg ggtgtccccc aaaaacggtc tctggataaa
      181 gcccaatcca cattgttttc cagccagtca ccaaagcgtt tttctcctac aaaaggtgga
      241 taccagataa ttttgcggtt attttctatt agttgttctt tataagccgt ggttttaata
      301 taccaactct cacgcgcata ataaataagg ggacttttac atctccagca aaaggggtaa
      361 ctatgtttta tctgttctct ttttaataag ttgcctcttt ctttcaagtg acgaataata
      421 tctttatcgg catctttgac aaactgatgt gcccaaggag ttactgcctc agtgaattta
      481 ccttcgctat ctaccggttg aacaaaaggc aaatcgtatt ttaatgctaa agaataatca
      541 tcctgaccaa acgcaggagc tgtatgcaca attcctgttc cttcactcat tgttacatag
      601 tcacccaaac agatatacca ggcgggttta tcaacactca caaaattaaa aaggggctga
      661 tagcaacgac cttccagttc cctgccttgc atttcggaaa taatctttgt ttcttcatcc
      721 agcacttgta agcgcgcttt agctaaaatt agattttgac cctgatattc aactttaagg
      781 taggtctctt caggatggac agctaaggca acattggaaa ttagagtcca gggagtagtt
      841 gtccaagcca agtagtaggt gttttcttca tccgttgctt taaacttaac ataaatggag
      901 gggtcttcta tatctttata accctgagct acttcatgcg aagaaagagg ggttccacag
      961 ctggggcaat aagggacaat tttatatgcc ttataaatga ggtttcggga gaaaaaatta
     1021 tgcaaaatcc accaaacgga ttctatataa ctattatgca aagttacata agggtgatcc
     1081 agatcgatcc aatagcccat cagttctgtc atttcacgcc aaagcttttc atagctgaaa
     1141 acggattccc gacatttttg acagaatttt tcaattccat attcctctat accgcgctta
     1201 tctttcagac cgagcatttt ttccacttca atttccacag gcaaaccatg agtatcccaa
     1261 ccggctttgc gtttaacctg aaagccgttc attgttttat aacggcaaat aacatctttc
     1321 agggtccttg ccattatatg atgaatacca ggtttgccat ttgccgtagg aggtccttca
     1381 taaaaaataa aacggggatt accttcacga atatcaatac ttttttgcac catatcgtgt
     1441 ttattccaat attcgcgaat gcggttttcc agatgcctgg gactttcttt caggtctatt
     1501 gttttataca tataccttat ccaattttct tttgatgacc acttctgcgt tgaccaggaa
     1561 caaaattatg cggattggca ggatggtttt tagtccgctc aaattccttt tgtttaagag
     1621 gattttcctg ttcactttcc actttttttt cttcaaagag ctcactgaag tgctccttca
     1681 attccttatg ttcagaaaca tattctttcc attcctcaat tgccttttct ctatcaacac
     1741 attttggaca gagaacaagc aaattttgtt cactaacacc gatccaacct ctgtcgtaac
     1801 agtgatcaca actttttttc ttacgatttt tttcggctat ggcttttgcc acatccaaat
     1861 gtatttcgtc aaggataaac atatctcctc ctttgcttac tatacagaga ggtttatata
     1921 tctctctcca caatttccct cattttgagc catttttttt aagtgcagaa ttctgtaaaa
     1981 ggaaattttc gcctcttctg tttacaggag cttctaaata aatatagggt gacaaatttc
     2041 aacttcaagc acagaaatca aaaagacaca taagtagtcc attctttgta tcttctgtaa
     2101 cagaattcag catctcacta tgattaaaca gataatcctc tgtcaaataa aaatgagagc
     2161 tcagaaatat tcagttcttg ggtgtgcgtt ttggttttga cttttccggg tttatcggta
     2221 aaggcgtaac ctcctcaatt ggctcttcct ctttaggagc gctttccata aattgaatgg
     2281 gagcactaat caccgttttc ttaagttcaa tttttacaat cggttccaaa cggtgcgtta
     2341 caataaataa gttcttattg cgggataaag caagtttatc ctctgcagat aatgcctcaa
     2401 tttc
//
LOCUS       DAOPFL010000173         2345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_64271_length_2345_cov_115.286463, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000173 DAOPFL010000000
VERSION     DAOPFL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64271_length_2345_cov_115.286463"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLG69_07640"
     CDS             <1..471
                     /locus_tag="PLG69_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPI43414.1"
                     /translation="LACLIGIQVMFIAPVIAQTMVSLSEEEFIADQSVVVIDSLQTQE
                     PPGDPPGEKMVATYYSKRFQGRKTASGERYNRDAFTCAHKTLPFQTLLKITNPRNSKS
                     VIVRVNDRGPFNRGRDIDLSYAAAKEIGMLSAGVIPVQVEILEQVYTDTLLAKN"
     gene            527..1444
                     /locus_tag="PLG69_07645"
     CDS             527..1444
                     /locus_tag="PLG69_07645"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPI43415.1"
                     /translation="MQLILHISPRIKRAFDKRKPILAMESTVLTHGLPYPKNLDILKT
                     LEELCQELDVEPATIIVLDGKIHIGMTEAEKEILQRKLESGEEVGKFNLRDIPYAVAN
                     KKSGGTTVSATMLLANKAGIKVFATGGIGGVHKFWNETLDISSDLKALAEIPVIVVSA
                     GCKAILDIPATLEVLESYSVPVLGWKTDEFPAFYARTSGKKIEKLESAKDVVNIYQTM
                     RELYPHPTGILVANPVPEKDEIPFAEIEKYISAAIAEAKTKNITGKELTPFLLATLAE
                     KTKGQSVQSNLALLKNNVIIGAQIAKELQ"
     gene            1441..2271
                     /locus_tag="PLG69_07650"
     CDS             1441..2271
                     /locus_tag="PLG69_07650"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HPI43416.1"
                     /translation="MKIYISTDMEGIPGTFNWEQEKTNRPEVLKNYQTHITDCLNAIL
                     YSGFKEFIEEITIADSHAGGDNLSYEITALDKRISLISGGPRPQYMMPALDSDYAMVF
                     LLGYHSGTGTWKGNMDHTYSNSKIFRIWINDIPMNEAIINSAYAGYKGVPVALISGDK
                     ALEEQIAVTMPWVHFVRTKEAVAKFAAKNYSSIIVKEDLVKAVQASLAQKDIPLYTFS
                     SPIRLKIEFHSTSMTDCAALMPHTKRLDGRTIEYVDDDYGVIFEAIMALVTLAYGATL
                     "
BASE COUNT          782 a          435 c          505 g          623 t
ORIGIN      
        1 ttggcttgcc taattgggat tcaggtaatg tttattgcgc cggtaattgc tcaaactatg
       61 gtaagcttaa gcgaggaaga gtttattgca gaccaaagtg tggttgtgat tgactctttg
      121 cagacccagg aaccaccggg agatcctcca ggtgaaaaga tggtcgctac ttattattct
      181 aaacgatttc agggcagaaa aacggcaagt ggcgaaagat ataaccgcga tgcctttact
      241 tgtgcccaca aaaccctccc ctttcaaacc ttattaaaaa taaccaatcc ccgaaacagc
      301 aaatccgtta tcgtgcgtgt aaacgataga ggtcccttca atcgtggtag agatatagac
      361 ctttcgtatg ccgctgccaa agaaataggg atgctttctg cgggggttat tcctgttcag
      421 gtggaaattt tagagcaggt ttatacagac actctgctgg caaaaaatta aaaacattat
      481 taattcttat tttcaacctg tcagggaaaa gggcaggtaa cttaagatgc aacttatttt
      541 acatatatct ccgcgaataa aacgggcatt tgataagcga aaaccaattc tggcaatgga
      601 atcaactgtg cttactcatg gcttgcccta tcctaaaaat ttggatatat taaaaacatt
      661 ggaagaactc tgtcaggagc ttgatgttga gccagctaca ataattgttt tggacgggaa
      721 aattcatatt ggaatgacag aagcagaaaa agaaattctc caacgcaaac tggaaagcgg
      781 ggaagaggtg ggtaaattta atttacggga tattccttat gctgtagcta ataaaaaaag
      841 cggagggaca accgtttctg ccacaatgct gcttgccaat aaagccggaa taaaggtttt
      901 tgccacaggt ggaatcggcg gagtgcataa attctggaac gaaacattgg acatttcttc
      961 agacctgaaa gctcttgcag aaattccggt gattgttgtt tctgccggat gtaaagccat
     1021 tttggatatt cctgccacgc tggaagttct ggaaagttat agcgttcctg ttttaggatg
     1081 gaaaaccgat gaatttcctg ctttctatgc aagaacaagc gggaaaaaga tagaaaaact
     1141 ggaaagcgct aaagatgttg taaatatcta tcaaacgatg cgagaattgt atccgcatcc
     1201 tacaggaatt ctggtggcaa atccggtgcc ggaaaaagat gaaattcctt ttgcggaaat
     1261 agaaaaatat atatcagcgg caattgccga agcaaaaaca aaaaatatta caggcaagga
     1321 actaactcct tttctgctgg caactttggc ggaaaaaaca aaagggcaat cagttcaaag
     1381 caaccttgct ctgctaaaaa acaatgtaat aatcggcgca caaatcgcta aggagctaca
     1441 atgaaaattt acatttctac cgatatggaa ggaatcccag gaacttttaa ctgggaacag
     1501 gaaaaaacca accgccctga agtcctaaaa aattatcaga cacatataac ggactgcttg
     1561 aatgcaattt tatatagcgg attcaaggaa tttattgaag aaataactat tgccgattct
     1621 cacgccggag gagataattt aagctacgaa ataaccgcat tggataaaag aatttcgctt
     1681 atttcaggcg gaccccgacc ccaatatatg atgcctgcct tagatagtga ttacgctatg
     1741 gtgtttttac tgggctatca ttccggcaca ggaacctgga aaggcaatat ggatcacacc
     1801 tattccaaca gtaaaatatt tagaatctgg ataaatgata taccaatgaa cgaagcaatt
     1861 ataaattccg cttatgctgg ttataaaggt gttccggttg ctttaattag cggagataag
     1921 gcattggaag aacaaattgc cgttactatg ccctgggttc attttgtcag aacaaaagaa
     1981 gcagtagcca aatttgccgc taaaaattat tcttctataa tcgtgaaaga ggacttagta
     2041 aaagccgttc aggcatcttt agcccaaaaa gatattcccc tctatacttt ttccagccca
     2101 atccgcctga agattgaatt ccactctact tctatgaccg attgcgctgc tttaatgcct
     2161 catacaaaac gccttgatgg aaggactata gaatatgtgg atgatgatta cggggttatt
     2221 tttgaagcaa taatggctct ggtaacactg gcttatggag ctactttgta aaaaagagaa
     2281 aagagaggtt attttacaaa gagagaaaga gaaaaagaga tttgatttta caaagaggga
     2341 aagag
//
LOCUS       DAOPFL010000174         2320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_65273_length_2320_cov_118.849007, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000174 DAOPFL010000000
VERSION     DAOPFL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65273_length_2320_cov_118.849007"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLG69_07655"
     CDS             <1..237
                     /locus_tag="PLG69_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43417.1"
                     /translation="GKVLAIGGLKEKLLAAKRAGIKRVVIPEENRETLSDFPADILAG
                     MEITYVQEIQEAIKILLIPNTESVEQKPKQRRIA"
     gene            240..1685
                     /locus_tag="PLG69_07660"
     CDS             240..1685
                     /locus_tag="PLG69_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPI43418.1"
                     /translation="MERYPNINILLNLPEKYLPKAEFVLRTYCYILRLNPTFLYGKHY
                     EGTHLYYGLPGKFDYPLKIYFQPETAEFFEKRELYPLEKVDFCKFRNEPIPFLFSCSG
                     AIFSFTEETCCFRKDIIASGFYFLTCWHEYILAYYGQTQGRIDYKQSLQYRWDFTETP
                     VVDVYCQMLLYAMEIYCPQFIREISWAEKKRFAVSLSHDIDYWDYWSGSAKLDVFKYN
                     LRTFIKRPFNSLYKITGHLLHKNLIYNPWKTMRSMTRRENEKDVRSTWFLLARKDFPD
                     KRQNYINDVKAKVQIMDLLGQQDVGLHGSPQSAFDPLVLAEELANLRDLGFNPTGFRT
                     HYLHFNYQQSFSILEEAGIKYDSTLGYWEHIGFRAGISFPFYPFNIAENRPFRVLEIP
                     LIIMDTTLFSQKAMNLSYISAKRALKRLIDTAEQYQSHLSLLWHNTTFDPIDYPMWGK
                     LYWHIIDYALKKQGWVTSLKDIYEEWVTLSY"
     gene            1803..>2320
                     /locus_tag="PLG69_07665"
     CDS             1803..>2320
                     /locus_tag="PLG69_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPI43419.1"
                     /translation="MKIISLLWEILFCLSFLIYLMIYFIYLILKLFLPRKVSKHILQT
                     IARFWAKMVVLSTGSKVVVTGKENLPSSGNICFVSNHQGLFDIPVILGFLGVHTGFVA
                     KRELFRIPVLSQWMREIPCTFIDRRNPRKAIQTFQKSAELIKKGNPMVIFPEGTRSRS
                     DKIGNFHLGSLK"
BASE COUNT          680 a          417 c          459 g          764 t
ORIGIN      
        1 ggaaaggttt tagccatcgg tggcttgaaa gaaaaacttt tagcggcaaa aagagcagga
       61 attaaacgcg tggtgattcc ggaagaaaat cgcgaaaccc tgtccgattt tcctgcagac
      121 attttggcag gaatggaaat aacttatgtg caggaaattc aagaggcaat caaaatattg
      181 cttattccca atacggaatc agttgaacag aaaccgaaac agagaaggat tgcctaattt
      241 tggaacgcta tcccaatatt aatattctct tgaatctgcc ggaaaagtat ttaccgaaag
      301 cggagtttgt cctccgcact tattgttata ttttgcgttt gaatcctacc tttctttatg
      361 gtaagcacta tgaaggcacg catctatatt acggattacc gggaaaattt gattatccgt
      421 tgaaaattta ttttcagccg gaaacagctg aattctttga aaagagggaa ttataccctc
      481 tggagaaagt ggatttttgc aagtttcgca acgagccgat tccttttctc ttttcctgta
      541 gcggagctat cttttctttt accgaagaga cctgttgctt tcgtaaggat ataattgcca
      601 gcgggtttta ttttttaacc tgctggcatg aatatatcct tgcttattat ggacaaacac
      661 agggaaggat tgactataag caaagcttgc aataccgttg ggattttacg gaaacaccag
      721 ttgtggatgt ctattgtcaa atgttgcttt atgctatgga aatttattgt ccacagttta
      781 tcagagaaat cagctgggct gaaaaaaagc gttttgctgt gtctctttct catgatattg
      841 attattggga ttattggagc ggttctgcca aattggatgt ttttaaatat aacctgcgga
      901 cttttatcaa acgacctttc aattcgctct ataaaattac gggtcatctt ttgcataaaa
      961 atcttatcta caatccctgg aagacaatgc gttctatgac acgccgcgaa aacgaaaagg
     1021 atgtccgttc cacctggttt ttattagcac gtaaagattt tccggataag cgtcagaatt
     1081 atatcaacga tgtgaaagca aaagttcaga taatggattt actgggtcag caggatgttg
     1141 gcttacatgg cagtccccaa tcagcatttg atcctttggt tttagctgaa gaattggcaa
     1201 atttgagaga cctgggattt aatccgaccg gcttcaggac tcattatctc cattttaatt
     1261 atcagcagag tttttccatt ttggaagagg cgggaataaa gtatgattcc actttgggtt
     1321 attgggaaca tataggtttt cgtgccggta tttcatttcc tttttatccg tttaatatag
     1381 ccgaaaacag accttttcgc gttttggaaa ttcctcttat tataatggat acaactcttt
     1441 tttcccaaaa agcaatgaat ttaagctata tttctgctaa acgtgccttg aaaagattaa
     1501 tagataccgc ggaacaatat caatcacatc tttctctact ttggcataat actacttttg
     1561 atccaattga ttatccaatg tggggtaaat tgtattggca tattattgac tatgctctta
     1621 agaaacaggg ttgggtaact tccctgaaag atatttatga agagtgggta acccttagtt
     1681 attaaacaaa ttactatgaa atcatatcat ttttttaatg aagagttcct tgtccttcct
     1741 gatgtctttg tccttcctac gaaagcagga atccataaat ttcagctaac ttcattctca
     1801 atatgaaaat aatatccctc ctttgggaaa ttctcttttg tctttctttt cttatctacc
     1861 taatgattta ttttatctat cttatcctga aacttttttt accgcgcaag gtttccaaac
     1921 acattttgca gacaatagcc aggttttggg caaaaatggt tgtgctttct accggcagca
     1981 aggttgtggt aacaggtaag gaaaatctgc cttccagcgg aaatatatgt tttgtcagta
     2041 accatcaagg tttgtttgat attcctgtca ttcttgggtt tttaggagtt cataccggtt
     2101 ttgtggctaa gagggaactt ttcagaattc cggtattgag tcaatggatg agggaaatac
     2161 cttgcacttt tattgaccgc agaaatcctc gtaaggcaat tcagaccttt caaaaaagtg
     2221 cagaactaat caaaaaaggg aatccaatgg ttattttccc ggaaggaaca cgcagcagaa
     2281 gcgacaaaat tggcaatttt catttgggaa gcttaaaatt
//
LOCUS       DAOPFL010000175         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_66026_length_2301_cov_90.144702, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000175 DAOPFL010000000
VERSION     DAOPFL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66026_length_2301_cov_90.144702"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(462..1052)
                     /gene="cas4"
                     /locus_tag="PLG69_07670"
     CDS             complement(462..1052)
                     /gene="cas4"
                     /locus_tag="PLG69_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HPI43420.1"
                     /translation="MNDFQFITPSEVMEYLFCPRFVYYMNIMKIEQHEHRRTLVNKGR
                     DIHKLKMVQNKEYLRKKAGAIDKLTDVYLSSEKLKLVGKVDEVLFLADGSAAPLDYKY
                     AFWENKVYKTLKYQQILYALLIMENFQVPVYKAFIVYTRSQNHLEELAITPKMLDKAK
                     LILDEIFMIINLEKYPKPAKAKRKCLDCTYRNLCSV"
     gene            complement(1049..1339)
                     /gene="cas2"
                     /locus_tag="PLG69_07675"
     CDS             complement(1049..1339)
                     /gene="cas2"
                     /locus_tag="PLG69_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPI43421.1"
                     /translation="MLTWVMYDIVKDKIRNKVSKCCEQAGIYRVQYSVFLGEISRTKC
                     KELSCQIEELINPEIDRVYIFPMCNEDFKSCQLLGQAFDPALITDEIKAFFL"
     gene            complement(1333..>2301)
                     /gene="cas1"
                     /locus_tag="PLG69_07680"
     CDS             complement(1333..>2301)
                     /gene="cas1"
                     /locus_tag="PLG69_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPI43422.1"
                     /translation="SPEKISSIVISNAAIITTDAIQLAMDYNIDIVFLDKYGSPYGRI
                     WFPKIGSTVLIRRRQLEMLSDNIGLQFIKNWIAIKIMNQYRFVQRLLSKRDCDKSNFE
                     NRMQNMQEAAICIMQAEGKLEELSGSFMGWEGGASKNYFSILAELIPDAYKFEGRSSR
                     PAKDAFNAMLNYGYGILYSKVERALIIAGLDPYLGLLHSDNYNKKSFVFDFIEPYRIL
                     VDEPVFYIFSRHKFSPDFIEPVHQGVLLSTAGKKFLAPLLLEHFDEIIRYRNKNRKRL
                     DMIQTDAHAFANFLIDKRENYLETSINRKLQNFLNSNFADNGEEEC"
BASE COUNT          779 a          436 c          363 g          723 t
ORIGIN      
        1 ttcttatata agtgcctcac aaataacaaa cttaaaccgg tttttcagat aatccaaact
       61 gatagttttg ataaaaaacc agctcccaaa tgagccaaaa ggcaatttcg ggcaggcaca
      121 aacactctat aaatctataa aagagcgaaa aggtaccaaa aaaatcaata aattcttctt
      181 agattaagat aaacggtatc tttaaaaagc aagtataatg ttaatttttt catccaaatt
      241 aaaacaatat cttattaatg ataagggacg aaagtaaatt agcattaata gttttcacta
      301 taaatctatg gattccacat caagggtgga atgataacac cattaaatta actaatgatg
      361 ttttattcat tctaacggca agagctggat ttccataatt ttctttctta ttccagcaaa
      421 cactctatac ataaaaacat atatgtgaat aaatctcata ttcagacaga gcacaaattc
      481 cgataagtgc agtccaggca ttttcttttt gctttggcag gttttggata tttttccaag
      541 ttaattatca taaaaatctc gtcaaggatc aatttagctt tatccagcat tttaggagtg
      601 atagccaatt cttccagatg gttttgagat ctggtataca caataaatgc tttatacacc
      661 ggaacctgaa agttttccat aatcagaagc gcatagagga tttgttgata ttttagggtc
      721 ttatagacct tattttccca aaaggcatat ttataatcca ggggtgcggc actgccatct
      781 gccaggaaaa gaacttcatc aacttttccc accagtttca atttttcgga ggaaagataa
      841 acatcagtaa gtttatcaat agcgcctgct tttttacgca gatattcctt attttgcacc
      901 attttcaatt tatgaatatc tctgccttta ttaacgaggg ttcttctgtg ttcatgctgt
      961 tcaattttca ttatgttcat ataataaacg aaacgagggc agaagagata ttccatcact
     1021 tcggaaggag tgataaactg aaaatcgttc ataagaaaaa tgccttaatt tcatctgtaa
     1081 taagagcggg atcaaatgcc tgaccaagta attgacagct tttaaagtcc tcattacaca
     1141 ttgggaaaat ataaacccgg tctatttcag gattgattag ttcctcaatc tgacagctta
     1201 attctttgca tttggtgcgg cttatttcac ccaaaaagac agaatattga actctgtata
     1261 ttccggcttg ttcacagcat ttggaaactt tattccggat tttatcttta acgatatcat
     1321 acatcaccca agttaacatt cttcttctcc attatctgca aaattgctgt tcaggaaatt
     1381 ttgcagtttt ctattaattg aggtttccag atagttttcc cttttatcaa ttaagaaatt
     1441 ggcaaaagcg tgagcatcgg tttgaatcat atcaaggcgt ttgcggtttt tattacggta
     1501 ccgaataatt tcatcaaaat gttctaataa caaaggagcc aggaattttt tccccgctgt
     1561 gctaagtaaa acaccttgat gaaccggttc aatgaaatca ggagaaaatt tatgccggga
     1621 aaaaatgtaa aaaacgggct catccaccaa aatacgatag ggctctataa aatcaaagac
     1681 aaaggacttc ttattgtaat tatcactatg caaaagcccc aagtagggat caagaccagc
     1741 aataattaaa gctctctcca ctttagaata gagaatgcca taaccataat tcagcatagc
     1801 attgaaagca tctttagcag gacgcgaact tctaccctca aatttatatg catcagggat
     1861 caattctgcc aaaatggaaa aataattctt ggaagcacct ccttcccaac ccataaaaga
     1921 accagaaagt tcttccaatt tgccttctgc ctgcattata caaatagccg cttcctgcat
     1981 attttgcatc cggttttcaa aattactttt atcgcaatca cgcttggaaa gcaaacgctg
     2041 cacaaaacgg tattgattca ttatttttat agcaatccaa tttttgatga attgtaaacc
     2101 tatgttatca gaaagcattt ccagctgcct tcttctaatt aaaacagtac tgccaatttt
     2161 gggaaaccaa atcctgccat aaggagagcc atatttatcc agaaaaacaa tatcaatatt
     2221 ataatccatc gctaattgaa tggcatcggt tgttattata gccgcattgg aaataacaat
     2281 agaagatatc ttttccggag a
//
LOCUS       DAOPFL010000176         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_66064_length_2300_cov_105.975501, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000176 DAOPFL010000000
VERSION     DAOPFL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66064_length_2300_cov_105.975501"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLG69_07685"
     CDS             complement(<1..887)
                     /locus_tag="PLG69_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43423.1"
                     /translation="MKYIYLIFALLACSILFAEPTYMPVTTIAEDFGATWCLNCPLAW
                     QGLQAVHSVTHYGEFISARLFTESGSLSNYMVDERFDYYNITGLPNVIFNGKISLEGI
                     GAGTADGTDYLNALSGYRYSASPVKIVPTNFSFERGLISGSVIMISPDVTISDARLVY
                     YLLEDNVGEEDHIVRGVLYDENVSLSGVGDTYNFNQTFDLNPSWVVSNLWGIVFLQLP
                     NKTILQCISSLPLPEYNFRAAMDWSRYITGDANTNYLSAPLWFFNLGAAENYTMHLEA
                     DNAPADWYFNYCDETGNCYP"
     gene            complement(1063..>2300)
                     /locus_tag="PLG69_07690"
     CDS             complement(1063..>2300)
                     /locus_tag="PLG69_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HPI43424.1"
                     /translation="KYSTEQTELLDELDSNRLELGWQELYASYEKDACKIYAGITEES
                     FGNGIVFRSYKDLEFDVDNRLESVLFSYNDAFKFKAIYGAIENPAIIGKYDLAYGADV
                     QSPYFQGLSLGASAMAFRNLLATNIYNQRTVFAGRLNYASKYVDFQAETAFSELYHQP
                     GIGTKHGKAIYVNGSYYFGPVTLGGAYKQYDKFQYRLNDLPAVNYHNETLSDASATGE
                     DEIGWQSFGTVNITEYLNFTADYAEAWNSDKDKKMNDAYIALEYVKDSFSLLASYSHI
                     EKIDDISNSWQQDLIPALQTNFALLKIPVQIQAEYKKVSKQRQEAESEHFEPKLQTDF
                     TLGKLSLSLCAQSNWEDISEILDSRYWASAQIKYPLFEHSDIILFGGKEAGGKVCRNG
                     VCRYVAPFEGLRFELNTRF"
BASE COUNT          720 a          504 c          402 g          674 t
ORIGIN      
        1 ggatagcaat ttcccgtttc atcacaataa ttaaaatacc agtccgctgg tgcattatcc
       61 gcttccagat gcatagtgta attttcagct gctccaagat tgaagaacca caaaggagct
      121 gaaagatagt tagtattggc atctcctgtt atataccgac tccagtccat agctgccctg
      181 aaattatatt ccggcaaagg cagagaagaa atacattgaa gaatcgtttt attgggaagc
      241 tgcagaaaaa caatccccca caagttactt accacccaag aaggatttaa gtcaaatgtc
      301 tggttaaagt tatatgtatc tccaacacca cttaaagata cattttcatc gtaaagaaca
      361 cctcttacaa tatggtcttc ctctcccaca ttatcttcca gcagataata tacaagacgg
      421 gcatcggaaa tcgttacatc tggcgaaatc attattacag acccgctaat caaacccctt
      481 tcaaaactga aattagtcgg cacaattttg accggtgaag cggaataacg gtaacctgaa
      541 agtgcattaa gataatcagt tccatctgca gttcctgctc ctattccctc cagagatatt
      601 ttaccgttga aaatcacatt aggcaaaccg gtaatattat aatagtcaaa cctttcatct
      661 accatataat tactgagaga cccgctttcc gtaaataacc tggcactaat aaattcgccg
      721 taatgcgtaa cggaatgaac tgcctgtaaa ccctgccagg caagaggaca attcaaacac
      781 caggtagctc caaaatcctc tgctatagtt gttacaggca tataagtcgg ctcagcaaaa
      841 agtatactgc aagccaatag agcaaatatc agataaatgt atttcatttt catctccttg
      901 tgtgttatat aataattgaa aaatgcaaaa ctgcaactgt atacaacgag gaagttgttt
      961 ttctttttcc atgatgactc ctaacttttc ctcatcaccc aagttataat cacaaattaa
     1021 atctcaaata aaaatctgcg taatctgcgt gaaatattct ttttaaaagc gggtgttaag
     1081 ttcaaatctt aaaccttcaa agggagcaac atagcggcag acaccattcc gacaaacttt
     1141 tcccccggct tcttttcctc caaaaaggat tatatcgcta tgttcaaaaa gcgggtattt
     1201 aatttgagcg gaagcccaat atctgctgtc taaaatttcg cttatatctt cccagttgct
     1261 ttgtgcgcat aaagaaaggg aaagtttacc caaagtaaag tccgtctgca gtttgggttc
     1321 aaagtgctca ctttccgctt cctgtctttg tttggagacc tttttatatt ccgcctgaat
     1381 ttgcaccggt attttcagca aagcgaagtt tgtttgcaag gcaggaatta aatcctgctg
     1441 ccaggaattg ctgatatcat ctattttttc aatatggctg taggaagcaa gcagagaaaa
     1501 ggaatcctta acatattcca gggcaatgta ggcatcgttc attttcttgt ctttatcgct
     1561 attccacgct tccgcatagt ccgcggtaaa attaagatac tccgtaatat taacggttcc
     1621 aaagctctgc cagccaattt catcttctcc tgtggcagag gcatcggaaa gggtttcatt
     1681 atgataattt actgccggca agtcatttag gcgatattga aacttatcat actgtttata
     1741 agcaccccct aaagttacag gaccgaaata gtaactgcca ttcacataaa ttgcctttcc
     1801 gtgtttagtt ccgataccgg gttgatgata taattcactg aaagccgttt ccgcttgaaa
     1861 atcaacatat tttgaagcat aattcaatct gccggcaaaa acagttcgct gattatagat
     1921 attggtggca agcaaattgc ggaaagccat agctgaagca cctaaagaaa gaccttggaa
     1981 ataaggactt tgcacatcgg caccataagc cagatcatat tttccgataa tggcaggatt
     2041 ttctatagca ccataaattg ccttgaactt aaaagcatcg ttataactga ataaaacact
     2101 ctccaaacgg ttatccacat caaattccag gtctttataa ctgcgaaaaa caatgccgtt
     2161 gccaaaactt tcttccgtta ttccggcata tattttacag gcatcttttt cgtaagaggc
     2221 atacaattcc tgccagccaa gttccaaacg gttgctatcc agttcatcca aaagttctgt
     2281 ttgttctgtg gaatatttgg
//
LOCUS       DAOPFL010000177         2266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_67455_length_2266_cov_100.582994, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000177 DAOPFL010000000
VERSION     DAOPFL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67455_length_2266_cov_100.582994"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="PLG69_07695"
     CDS             <1..1826
                     /locus_tag="PLG69_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPI43425.1"
                     /translation="KPVLNKLGSSKWNNTKRKAAQQIELIAADIVKLYAERSARLGIA
                     HQPDTEWQKELEDSFIYEDTPDQSKATKEIKEDMELPAPMERLLCGDVGFGKTEVAIR
                     AAFKAVCSGYQVAVLAPTTLLVEQHYRVFRERLAQYPVKIVMFSRFRKNTAMKKDLLG
                     LKTGSIDIAIGTHRLLSKDVQFKKLGLLIIDEEHRFGVRHKEKLRALQSNVDTLYMSA
                     TPIPRTLNMALSKLKEISLMQTSPKERLPVRTIITPRDMEVIKDAIRREIDRGGQVFF
                     IHNRVQTIETVATELRNAMPKVRFIVGHAQMPEQQLERVMADFWAKEYQVLISTTIIE
                     NGIDIPNANTILIDNAETFGLAQLYQMRGRVGRSNRRAYAYLLISKGTTTVARKRLEA
                     LTQYDYLGAGFQVALRDLELRGAGTILGTKQSGIIQAIGFNYYNRILANAIQAVEKGE
                     TTKLFNDESPDTRRKVRTEIDLYFPPDYIDDDEERLRIYKRLSELETLEDINNFEVEL
                     LDRFGPLPEQASWLLNYFQLSQLAKKLSLKDCQVKHDSFIMEFDAKKLPSKEQLLHFT
                     TKIKQPMRFEAGKNLKVIIDLDPEASYLQQFETAIEILKIW"
BASE COUNT          753 a          411 c          493 g          609 t
ORIGIN      
        1 ccaaaccggt tttaaacaaa cttggcagtt ccaaatggaa taatactaag cgaaaagctg
       61 ctcaacaaat tgaacttatt gcagccgata ttgttaagct atatgcagaa agaagtgcac
      121 gccttggtat tgctcatcaa ccggataccg aatggcaaaa ggaattggag gattctttta
      181 tctatgagga tacccccgat caaagcaaag ccacgaaaga aattaaagaa gatatggaac
      241 ttccggcacc gatggaacga ctgctttgtg gagatgttgg tttcggaaaa acggaagttg
      301 ctatccgagc tgcatttaaa gctgtctgca gtggctatca ggttgccgtt ttagcaccta
      361 caactttatt ggtagaacag cactataggg tttttagaga acgcttggct cagtatcctg
      421 ttaaaattgt aatgtttagc cgttttagga aaaacaccgc tatgaaaaaa gaccttttag
      481 ggcttaaaac tggctctatt gatatcgcta tcggaaccca tcgtttgctt tccaaagatg
      541 ttcaattcaa gaaattggga cttttaatta ttgatgagga acatcgtttc ggagtgcgcc
      601 ataaagaaaa actaagagcc ctccaaagca atgtggatac actttatatg agcgcaactc
      661 ccattccgcg aactttgaat atggcacttt ccaaactgaa agaaatttcc ctgatgcaga
      721 cctcgccgaa agaaagatta ccggttagga ctattattac cccacgggat atggaagtaa
      781 tcaaagatgc catcagaaga gaaattgacc gaggaggaca ggtttttttc attcataacc
      841 gtgtgcagac aattgaaaca gtggcaacgg aattaagaaa cgcaatgcct aaagttcgtt
      901 ttattgtagg tcatgctcaa atgccggaac aacaactgga aagggtaatg gctgactttt
      961 gggcaaagga atatcaggtg ctaatttcta ctactatcat tgaaaacggt attgatatcc
     1021 ctaatgccaa cacgatttta atagataatg ctgaaacatt cggtttggca caactttatc
     1081 aaatgcgcgg tagagtagga agaagtaacc gaagagctta tgcctatctg cttatttcca
     1141 aaggaactac aacagttgcc agaaaacgct tggaggcatt gacccaatat gattatttgg
     1201 gtgccggctt tcaagttgcc ttacgagatt tggaattaag aggtgctgga accattttgg
     1261 gaactaaaca aagcggaatt attcaggcaa ttggctttaa ctattataat cgcattctgg
     1321 ctaatgccat tcaagctgtg gaaaaaggcg aaacaaccaa attatttaat gatgaatcac
     1381 cggatacaag aagaaaagta agaactgaaa tagacctcta ttttcctccc gattatattg
     1441 atgacgatga agaacgcctg cgtatttaca aacgcttaag cgaattggaa acgctggaag
     1501 atataaataa ttttgaggtg gaacttttag accgcttcgg acctttacca gaacaggcaa
     1561 gttggcttct gaactatttc cagctgagcc aattagctaa aaaattgtct ttaaaggact
     1621 gccaggttaa gcacgatagc tttattatgg aatttgacgc taaaaaacta ccttctaaag
     1681 aacaattgct ccattttact accaaaatta aacaaccaat gcgctttgaa gcaggaaaaa
     1741 acctgaaagt tattattgat ttagaccccg aagcaagcta tctacaacag tttgaaacgg
     1801 cgatagaaat cctgaaaata tggtaacctg caaggggatt tttacaccga agacaccgag
     1861 agattttatt ttacaaagag aaaagagaga aagagataaa ttgagagtga agaatgaaaa
     1921 aggttgagag ggtttttcta ttttaactca caatttttag gttaggaaac cagttaaaat
     1981 gcggttgtaa gtgagatagt tcgtaaattg tagttttctg gaaggcaggt aaaattgtaa
     2041 gtccaaattt tctcagttga aacatcttga atttgacgag gggtttacac acccatcgct
     2101 atgattgtgt cgccctgacg ggcttttttt agtgggaaag aaagaaagaa aaaatgtcgg
     2161 ggctttcatt ttaacgaggg gcttacgcca ccatcgctat aaaagtttcg ccctgacggg
     2221 cttcttggct gagggcaaag agctacgagc agagggaatc tcaacc
//
LOCUS       DAOPFL010000178         2226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_69178_length_2226_cov_65.148319, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000178 DAOPFL010000000
VERSION     DAOPFL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69178_length_2226_cov_65.148319"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2226)
                     /locus_tag="PLG69_07700"
     CDS             complement(<1..>2226)
                     /locus_tag="PLG69_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gingipain R"
                     /protein_id="HPI43426.1"
                     /translation="PTAMVYVGTPNSFIVTAPSTIPSGSINIRIGVTNGNGEIVEGAF
                     VTLTTTSGLQLTGITDASGFVTIDLPAGLSQNLVLTVAKDDYIAYTSSITLNTGGGIV
                     YDSFVLDDDTTTGNGDGTLNAGEEVNLYVTLKNTSSSTLFINGRVSTTDSYVTLIEYD
                     RLEFGDIVAGGLGENITPIVFSVSPACPDQHIITLVLTAEGAGNNWTINIPLTVRGGN
                     LEIQSYTFVGITGNIITPGLSSPFTFSLKNTGISTLHNVYGNLQSLSNYFVVQDAEGY
                     FGNIAPNSTVTNSSNSFTVFAWDSSINGMVIPLILTLSNANGYTQTLHLSITIGTTTV
                     HDPLGQDAYGYFIYDQTDTAYPLCPSYQWIPIAPAEGGSGTLLSLTDPGNTSDEGDDT
                     SAIAIQTVNLPFTFTFYGRQYTQASICSNGFIAFGATQNADWRNWRLPGAGGPNPMIA
                     VFWDDLQLGTGSGVYTYYNSAQHYYVVEWYNIISGYNGSSLETFQAILYDPVYYPTIT
                     NDGQIKLQYKVFNNIDQGSGDVYPHGNYCTIGIKDHYGTDGLEYTFNNTYPTAASPLS
                     NEKALLISTPEIISGTPHLTIAQTTVIDPNSNNHLEPGETAQLQILLRNNGLSTAENV
                     TGVLSCSDSYVTINNATANYGNIAGEAQGLPQSNFNLSVSPSCPGGHRINFTLALSGS
                     GLNWNYSFSLDVYVPILEFGNYTVLDSNGDNDGILDPGETVNLSVQLLNTGEVASPAG
                     TV"
BASE COUNT          729 a          440 c          418 g          639 t
ORIGIN      
        1 taccgttcca gccgggctgg ctacttctcc tgtattcagt aattgaacag agaggtttac
       61 tgtttcgccg gggtctaaaa ttccatcatt gtctccatta gaatctaaca cagtatagtt
      121 tccgaattcc agaatcggaa catagacatc taaagaaaag gaatagttcc aatttaatcc
      181 actaccggaa agagccagag taaaattaat tctatgtcct ccagggcagg atggagaaac
      241 acttaaatta aaattacttt gtggtaagcc ctgtgcctct ccggcaatat ttccataatt
      301 agcagtagca ttattaattg tgacataaga atcggaacaa ctcaacacac cagtaacatt
      361 ctcagcagta gaaagtccat tgtttctaag caggatttgc agttgagctg tttcacccgg
      421 ttccagatga ttattactat tagggtcaat aacagttgtt tgagctatag taagatgagg
      481 tgtaccggat ataatttccg gtgtgctgat aagcaaagct ttttcgttac tcaagggact
      541 cgcagctgta ggataagtat tgttgaaagt atattccaat ccatcagttc cgtaatggtc
      601 tttaatacct atagtgcagt aatttccatg cggatataca tctccactac cctggtctat
      661 attattaaaa accttatatt gtaattttat ttgaccatca ttggtaatag ttggataata
      721 aacagggtca tataatatcg cttgaaaggt ctccaaactg gaaccgttat agccagaaat
      781 tatattatac cattccacta cataataatg ttgagctgaa ttataatatg tataaactcc
      841 acttccagtt cccagttgaa ggtcatccca aaagacagca atcatcggat tgggacctcc
      901 tgctcctggt aaacgccaat ttctccagtc cgcattttgg gtcgcaccaa aagcaataaa
      961 accattggaa caaatggatg cctgagtata ttgtctacca taaaaggtaa aggtaaaggg
     1021 taaattaacc gtctgaatag ctatagcact tgtatcgtca ccttcatctg atgtatttcc
     1081 cgggtctgtt aaagatagta aggttccact tcctccttca gcaggagcaa taggaatcca
     1141 ttgataactt gggcacaaag gatatgccgt atccgtttga tcataaataa aatagccata
     1201 agcatcctga cccaaaggat cgtgaacagt cgttgtgcca atcgtaatac ttaaatgtaa
     1261 ggtctgggta tatccattag cattagaaag tgttagaatc aacggaatca ccattccatt
     1321 tatactgcta tcccaggcaa aaacagtaaa gctgttagaa ctattagtaa ctgtagaatt
     1381 aggagcaata ttgccaaaat aaccctcagc atcttgaaca acaaaatagt tactgagcga
     1441 ctgcaaattg ccgtaaacat tatgtaaagt agaaataccg gtgttcttta gggaaaaagt
     1501 aaagggactg gaaagtccgg gagtgataat attgcccgtt atacccacaa aagtataact
     1561 ttgaatttcc aggttgcctc ctcttacggt cagagggata tttatcgtcc aattgtttcc
     1621 ggcaccttca gccgtaagca ctaaagtaat aatatgttga tcaggacagg caggggaaac
     1681 ggaaaaaaca ataggagtaa tgttttctcc caaaccacca gcaacaatat caccaaattc
     1741 caggcggtca tattctatta aagtaacata agaatctgta gtagataccc taccattaat
     1801 aaagagagta gaagaacttg tatttttcag tgttacatag aggttaactt cttctcctgc
     1861 attcagtgtt ccatctccat tgccagtagt tgtatcatca tccagaacaa aactgtcata
     1921 gacaattcct ccaccagtat ttaaagttat ggagctcgta taagcgatat aatcatcttt
     1981 agcaacagtt aaaaccaagt tttgggaaag tcctgctggt aaatcaatag taacaaatcc
     2041 cgaagcatct gtaattccgg ttaattgtaa accggaagta gtagttaaag ttacaaaagc
     2101 accttctaca atttctccgt taccgttggt tactcctatt ctgatattga tagaaccgga
     2161 aggaatggtt gacggagcag taacaataaa actattggga gttcctacat aaaccatagc
     2221 agtagg
//
LOCUS       DAOPFL010000179         2219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_69469_length_2219_cov_103.913124, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000179 DAOPFL010000000
VERSION     DAOPFL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69469_length_2219_cov_103.913124"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLG69_07705"
     CDS             <1..522
                     /locus_tag="PLG69_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43427.1"
                     /translation="DATREATDILDTLSSANRESASRKTKIRELEKELEIEKEKTEKM
                     NDPEKQKEFEQFKSKAEQYDQYLANQNQEILSSWKAANEKLASIKETDKRYERVKTVL
                     GKLKKAEEGKELSAADAKYNLDLYDIMVSTGVLSDEGKGSYPNYPQSKAQDGMTPQTT
                     ADAIIGLAKKPIK"
     gene            538..1500
                     /locus_tag="PLG69_07710"
     CDS             538..1500
                     /locus_tag="PLG69_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43428.1"
                     /translation="MATTTSNLQALAAAWGVGNEYQPIVVDLLKRSSLLQTATVAKAS
                     HGIKHKFRYFNSLPTAAFREIGEGIVPQKVDVNTAQIDLKELVFDLFDDYQAILQYPG
                     GKEGWLKDNYTAALAALTNALAKAVFYGNIPSFGYEKAFKGFHQYAKDLGQVIAQKGA
                     TTAYRSSIFAVRWDEFDGASLRFNNTELLQVIDMTPNQPIPIVTDTTTNKQMNIFKWI
                     FSAYFTLIIPSAKSVAVITQIDTSHLPTADDMNALIDAVEADSGNTVIYCNATARRAI
                     AKIKDVKLNMYAESSDYNNNVATWRGVPIHREDALVATETTDLD"
     gene            1515..1931
                     /locus_tag="PLG69_07715"
     CDS             1515..1931
                     /locus_tag="PLG69_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43429.1"
                     /translation="MAIYQKYGFAIDQILSENQALPNTTSGDSTNTIKLDAVADDGLH
                     IVVCAASTTVELASGATLEIRPTIGATDGTVTTVLPSILITQGVQSDVSWASGEMICQ
                     FNIPAKLIGSARYLKLTYVTSANESDDKVEAFSVRR"
     gene            1990..>2219
                     /locus_tag="PLG69_07720"
     CDS             1990..>2219
                     /locus_tag="PLG69_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43430.1"
                     /translation="MKWNDTTLLSTLETISRWEKEVNELAGYDSIPAIQGNKTAKIID
                     INKSIDSIVYNDSAGISHTSEIKVAGEWDIPEA"
BASE COUNT          750 a          400 c          458 g          611 t
ORIGIN      
        1 gatgccacga gagaggcaac tgacatattg gatacactct caagcgcaaa tcgggaatct
       61 gcttcccgaa aaacaaagat acgagaactg gaaaaggaat tagaaataga aaaagagaaa
      121 acagaaaaga tgaatgaccc tgaaaaacaa aaagaatttg agcagttcaa atccaaagca
      181 gaacaatatg atcagtatct tgctaatcaa aatcaggaaa tactttccag ttggaaagca
      241 gcgaatgaaa aattagcaag cattaaagag accgataaaa gatatgagcg agtaaagaca
      301 gtattgggca aactaaagaa agcagaagaa ggtaaagagc tttctgctgc cgatgcaaaa
      361 tacaatctcg atctctatga cattatggtc tcaacgggag ttctatctga tgaaggaaaa
      421 gggagctatc ccaattatcc tcagagtaaa gctcaagacg gtatgactcc gcaaactacg
      481 gctgatgcaa taatcggctt agcaaaaaaa ccaataaaat aaaaaaagga aattattatg
      541 gcaacgacaa cttcaaactt acaggcatta gctgctgctt ggggtgtagg aaatgagtat
      601 cagcccattg tggttgattt actaaaacgc tcatcgctct tgcagaccgc cacagtagca
      661 aaagcttcac acggaattaa acacaaattc cgttacttca attcacttcc tactgctgct
      721 ttcagagaaa tcggagaagg aattgttcct caaaaggtag atgtaaatac tgcccaaatt
      781 gacctcaagg aattggtctt tgatttgttt gacgattatc aagcaattct tcaatatcct
      841 ggtggcaaag agggctggct taaagacaat tatactgcgg ctttggctgc attgaccaat
      901 gctttagcga aagccgtctt ttatggcaat atcccgagtt ttggttatga aaaagcattc
      961 aaaggattcc atcaatacgc taaagatttg gggcaagtaa tagctcaaaa gggagcgaca
     1021 actgcttatc gttcttcaat ctttgcagtt agatgggatg aatttgatgg tgcttctctg
     1081 cgtttcaata atacagaatt gctccaggtt attgatatga ctccgaatca gcccattccg
     1141 attgtaactg atactacaac aaataagcag atgaatattt tcaaatggat attttctgct
     1201 tactttacgc tgattattcc cagtgcgaaa tctgtagcag ttattactca gattgataca
     1261 tctcatttac ctacggcaga tgatatgaat gccttgattg atgcagtaga agctgattcc
     1321 ggcaataccg tcatttattg taatgccact gctcgtagag caatagctaa aatcaaagat
     1381 gttaaattga atatgtatgc tgaatcatct gattacaata acaatgtggc tacttggcgt
     1441 ggcgttccta tccatcggga agacgctttg gtcgctaccg aaactactga tttggattag
     1501 aaggaaggta aataatggct atttatcaaa aatatggatt cgcaatagat cagattctgt
     1561 ctgaaaatca ggcattacct aatactactt ctggagacag cactaatact attaaattgg
     1621 atgcagttgc agatgacgga ctacatattg tggtctgtgc tgcttctacc acagtagagc
     1681 ttgcttcggg tgctactctg gaaatcagac ctacaatagg tgcaacggat ggtacggtta
     1741 caactgtttt acccagtatt ttgattacac aaggtgttca atcggatgtt tcctgggcct
     1801 ctggtgaaat gatttgtcaa ttcaatattc cggcaaaatt aattggttct gccagatatt
     1861 tgaaattgac ttatgttacc agtgccaacg aaagtgatga taaagttgaa gctttttcag
     1921 tgcgtagata aagtaataat tcagggaggc agagataagg ataatctgcc tcccaatatt
     1981 gagtgaaaaa tgaaatggaa tgatactact cttctttcaa ctctggaaac aatctccaga
     2041 tgggaaaaag aggtcaatga attagctgga tatgattcta ttcctgcgat tcaaggtaat
     2101 aaaacagcta aaatcataga tataaataaa tctattgatt ctattgtcta taatgattca
     2161 gccggtatat ctcatacttc agaaatcaag gttgccggag aatgggatat tcccgaagc
//
LOCUS       DAOPFL010000180         2216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_69588_length_2216_cov_83.298936, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000180 DAOPFL010000000
VERSION     DAOPFL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69588_length_2216_cov_83.298936"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1044)
                     /gene="tsaD"
                     /locus_tag="PLG69_07725"
     CDS             complement(28..1044)
                     /gene="tsaD"
                     /locus_tag="PLG69_07725"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPI43431.1"
                     /translation="MPVQYILAFESSCDDTSVAIVDTDYNVIVNLISSQPEHLEFGGI
                     LPELASRLHLKNIVTLTKVALNASKLNLQDISAIAVSINPGLIGSLIVGLAFAKGLAW
                     SLSLPLITVNHILSHIFANFIEHKAVEPPFLALVVSGGHTELVHFDTLTTFTVVGKTL
                     DDAAGESFDKAAKLLGLGFPGGPAIDELAQKGNPNFIKFPRALPQKNNFNFSYSGLKT
                     SIRTWLVNQNPETLQDKLPDIAASVQQAIIEPLVHKTVLWARQHKIPYILLAGGVAAN
                     SALRQQLTAASAKYGIKVFYPSNTLCMDNAAMVGAAAIPKFLAKNYAPLSINVSSLKG
                     TRQV"
     gene            complement(1045..1920)
                     /locus_tag="PLG69_07730"
     CDS             complement(1045..1920)
                     /locus_tag="PLG69_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPI43432.1"
                     /translation="MLQENLGLKILSLLIAVFVWLQSLLISEQKSVVNLPVALSSIPK
                     NITLDNVPNHIPFNVRGRGLEIIKLKFSRTKVLLDASKIKPGSDILSVSDYTIDLQEN
                     IQVEILGPVEQREISVYADVFHQKKVPVELSFADSYTRKHFTALNYTIIPEQITISGP
                     KSKVQQIDRVTTEPVSREMLEQREVTLKINPLGSEVSASEKQVKIYLSTSYITTKIYH
                     NLPLTVPPGKSSIPSSVTVKLSGNSDILQNLKPTELKVSIASQPDEQGFYPVLIEIPD
                     TVKLIAVTPQKVRLK"
     gene            complement(1923..>2216)
                     /locus_tag="PLG69_07735"
     CDS             complement(1923..>2216)
                     /locus_tag="PLG69_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPI43433.1"
                     /translation="CKFIMINEQKINNNVIKKHLQQKINQFRFSKNYSSEKYTKIHNL
                     KDSVASFERDYLLYYLSLNNWRISQTAEAIGLERTTLYKKIKSLGINHLKMED"
BASE COUNT          678 a          377 c          471 g          690 t
ORIGIN      
        1 cagttttcat ttccgtgagc ggtattttta tacttggcgt gttcctttga gggaggagac
       61 atttatggaa agtggagcat aattttttgc taaaaattta ggaattgctg ctgctccaac
      121 catagccgca ttatccatac aaagagtgtt tgaaggatag aaaactttga ttccatattt
      181 tgctgatgct gctgttagct gttggcgtaa agcagaatta gctgcaactc cccctgccaa
      241 aagaatatag ggtattttat gctgtcttgc ccaaagcact gttttgtgca ctaagggttc
      301 aattattgct tgctgaacgg aagcagcgat atcgggaagt ttatcttgaa gcgtttccgg
      361 attctgattt acaagccagg tacgaataga tgtttttagt ccactgtaac tgaaattgaa
      421 gttatttttt tggggtaagg cacgaggaaa tttaataaaa ttggggttgc ctttttgggc
      481 taattcatca atagcaggac caccgggaaa gcccaaacca agaagttttg cagctttatc
      541 aaaactttct ccagcggcat catccagggt ttttcctact acggtaaaag tggttaaggt
      601 atcaaaatgg acaagttctg tatgccctcc ggaaacaaca agggctaaaa aagggggttc
      661 aacagcttta tgttcaataa aattggcaaa aatatgcgac aagatatgat taaccgttat
      721 taaaggtaaa gacaagctcc aggcaagtcc tttggcaaaa gccagaccga caattaacga
      781 accaattaaa ccgggattga ttgaaacagc tattgcactg atatcttgta agttaagctt
      841 agaagcattg agagccactt tggttaaggt aactatgttc tttaaatgca agcgggaagc
      901 cagttcgggt aaaatacctc caaattccag atgttccggt tggctggaaa taaggtttac
      961 aattacatta taatccgtat ccacaatggc tacagaggta tcatcacagg aggactcaaa
     1021 agccaaaata tattgaacag gcatttattt caggcgcacc ttttgaggtg taacagcaat
     1081 caatttcact gtatcgggta tttctataag gacaggataa aagccctgtt catcaggttg
     1141 ggaagcaata ctaactttga gttctgttgg cttcaggttt tgtaaaatat cagaattacc
     1201 ggagagtttt acagttacag atgaaggtat agagcttttt cccggtggca cggtaagagg
     1261 tagattatga taaattttgg tggtaatata ggaagtggag agataaattt taacctgttt
     1321 ttcagaagcg gagacctcac ttcccaaagg gtttattttt aaggtaactt cacgctgttc
     1381 aagcatttcg cgggaaacag gttcggtagt aacgcgatca atttgttgaa ctttgctttt
     1441 aggaccggag atggtaattt gttcaggaat aatagtataa tttagggcag taaaatgctt
     1501 acgggtatat gaatcggcga aagaaagttc taccggcact tttttctgat ggaaaacatc
     1561 agcataaacc gaaatttccc tttgctctac tggacctaaa atttctactt gaatattttc
     1621 ctgcaggtca atagtataat cgcttacaga aagaatatcg gaaccgggtt taattttgct
     1681 ggcatcaagc aagaccttgg tgcgggaaaa ttttagtttg ataatttcca agccccttcc
     1741 acgcacattg aaaggtatgt gattgggaac attatctaaa gtaatgtttt taggaataga
     1801 acttagggct acaggtaaat taactacgct tttctgttct gaaatcaaca aggactgcaa
     1861 ccaaacaaaa acagctatta atagcgagag gatttttaaa cccaggtttt cctgcagcac
     1921 ttttagtcct ccatctttag atgattaatt ccgagggatt tgattttttt atacaaagtg
     1981 gtgcgttcaa gtcctattgc ttctgcagtt tgggaaatcc tccaattgtt caaggataaa
     2041 taatacaaaa gataatcccg ttcaaaagaa gcgacactat ccttgagatt atgaattttg
     2101 gtatattttt cgctgctata gttcttagaa aagcggaatt ggtttatttt ctgctgaagg
     2161 tgttttttaa taacattatt atttattttc tgttcattaa tcataatgaa cttgca
//
LOCUS       DAOPFL010000181         2205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_70053_length_2205_cov_62.968372, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000181 DAOPFL010000000
VERSION     DAOPFL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70053_length_2205_cov_62.968372"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2200
                     /locus_tag="PLG69_07740"
     CDS             <1..2200
                     /locus_tag="PLG69_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPI43434.1"
                     /translation="NFNGNDALALIKISTGAYVDIFGVIGNDPGTAWLGENNYTTINS
                     TLVRKSSVAQGVTQNPTGTGAYAFTTLVTEWDLYPVDTIDNLGFHNFAATPQEIEPPT
                     VQASNLIAYPGNTDITLEWTPGNGARRLIKINTINSFTPPVDGSNPTANNVYSGNGEQ
                     VIFNGATQIVEGIPLNGCNVSGLTPATTYWFRSYEYNGSGSNTRYNTSEAINNPISAT
                     TTNTQSSGYYAGITGYGNTLKTNLHNLLRTTHTTRYSYDALWIKLPYTDEDPNNTDNI
                     IEIYTGWSVPKSYYGGGTSQWNREHTWSKSHGDFGETPPAGTDLHHLRPCDSTVNSAK
                     SNKDFDYAPNPYIDSSPYNGYSSDTGCKTSTYAWEPRDEDKGDVARMIMYMAIRYEGT
                     DTSFDLEIVDNINTSGPNYGKLSTLLQWHIIDPPDAREMQRNNRIQELQGNRNPFIDE
                     PMYAYQIWAPMPLNPTNITQTGFTANWTAPISATKYYFQLATDANFTNIVPGYDNLDV
                     GLTLSRNISGLSNGYTYYYRLRSYFTSGYSMYSPYQEVTLTLPASAQLSSGTALTEGN
                     LNGAILQLTLTNTTWRDNTLLISSFTLNNAPAGLSLLSVQYLSATAAQIVLSFDNTDF
                     DANILNFSVTINALEINNGSNLTTNTLTIIAYIECPLSIQIQGSQLILNIQEVSGANL
                     YKVFSSTDPYGVYTEISTSGIFDPLVPNRWSYTVPADERRFYRSAAVRNP"
BASE COUNT          658 a          457 c          413 g          677 t
ORIGIN      
        1 taattttaac ggtaacgatg ccttagccct cataaaaata agcacgggag cttatgttga
       61 tattttcggt gttatcggaa acgaccctgg aactgcctgg ttaggtgaaa acaattacac
      121 tactataaat tctaccttag taagaaagtc ctctgtagct caaggagtaa ctcaaaatcc
      181 aaccggcaca ggtgcttatg cttttactac tttggttacc gaatgggatt tataccctgt
      241 ggatacaata gacaatcttg gctttcataa tttcgctgcc acacctcaag aaatagaacc
      301 gccaacagtg caagcttcaa atttaattgc ttatcctggc aatacggata taactttgga
      361 atggactccg ggaaatggag caagaagatt aattaaaatc aatactatca attcatttac
      421 ccctcctgtt gacggtagca atcctactgc caacaatgtt tacagtggca acggagaaca
      481 ggttatcttc aatggagcaa ctcaaattgt ggaaggaatt ccgttaaatg gctgtaatgt
      541 atccggtctt actcctgcaa ctacatattg gttcaggagt tatgaataca acggtagtgg
      601 cagtaatact cgttataata cttcggaggc aataaacaat cctatttccg caactacaac
      661 aaatacccaa agcagtggct attatgccgg aattacaggt tatggaaaca cgctgaagac
      721 aaatctgcat aatttacttc ggacaacaca taccactcgt tattcttatg atgctttgtg
      781 gattaaattg ccctacacgg atgaagaccc taataatact gataatatta ttgaaatata
      841 caccggttgg agtgttccca aatcctatta tggaggagga acttcacaat ggaatagaga
      901 gcatacctgg agtaaaagtc atggtgattt tggggaaaca cccccggcag gaacggattt
      961 gcatcatttg cgtccctgcg attctactgt aaattctgcc aaaagtaata aggattttga
     1021 ttatgctccg aacccttata ttgattcttc accctataat ggttactctt cagatactgg
     1081 ctgtaaaacc tctacttatg cctgggaacc gagagatgag gataaaggtg atgttgccag
     1141 aatgattatg tatatggcaa tccgttatga aggaacagac acttcttttg atctggaaat
     1201 tgtagataat ataaatacca gcggacctaa ttatggaaag ctttctactt tactgcaatg
     1261 gcatattatt gatcctccgg atgcccgtga aatgcaacgt aataatcgta ttcaggaact
     1321 tcagggaaat cggaatccct ttattgatga gccaatgtat gcctatcaaa tttgggctcc
     1381 tatgccttta aatccgacga atattactca gacaggtttc actgctaatt ggacagctcc
     1441 catttctgct acaaagtatt attttcagct ggctactgat gccaatttta cgaatattgt
     1501 tcccggttat gataatctgg atgtgggttt aactctttcc cgcaatatca gtggtttatc
     1561 taatgggtat acttattatt atcgtttgcg ttcctatttt acaagtggtt acagtatgta
     1621 ttcaccttat caggaagtta ctcttacctt acctgcatcg gcacaacttt cttccggaac
     1681 cgcattaacg gaaggaaacc tgaatggagc tattttacaa ttgactttaa ccaataccac
     1741 ctggagagat aacaccttgc taattagtag ttttactcta aataatgctc ctgcaggtct
     1801 ttctctttta agcgtgcaat atctcagtgc tacagccgct caaatagttt taagctttga
     1861 taataccgat tttgacgcta atattcttaa ttttagcgtt accatcaatg ctttagaaat
     1921 aaataatggc tccaatctaa ctaccaacac tttaacgatt atagcttata tagaatgccc
     1981 tctatccata cagattcagg gttcgcagtt gatattaaat atccaagagg tctccggcgc
     2041 caatctttac aaggtctttt ccagcactga tccctacggt gtttatacgg aaataagcac
     2101 cagcggaatt tttgatcctc ttgttcccaa tcgttggagt tataccgttc ctgctgatga
     2161 acgcagattt tacaggtctg ctgctgtccg caatccttaa tgatc
//
LOCUS       DAOPFL010000182         2182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_71064_length_2182_cov_121.517160, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000182 DAOPFL010000000
VERSION     DAOPFL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71064_length_2182_cov_121.517160"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /gene="rpsB"
                     /locus_tag="PLG69_07745"
     CDS             <1..630
                     /gene="rpsB"
                     /locus_tag="PLG69_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPI43435.1"
                     /translation="EYILFVGTKKQAQAAIKEAAEKAGVFYVNQRWYGGMLTNMATIR
                     QSIEKMKYYEEIVADGTINGYTKLEQQKMKRMHDKIEFSLGGIREMDALPGCIFIVDT
                     EYEKIAVHEARILNIPIVAMVDTNCDPDLVDYVIPSNDDATRAIHLISDIMANAVIEG
                     KGLATEGESTEEETTETTAEEETEDFEAELAAAEEKVEFDVPELAETEQ"
     gene            673..1272
                     /gene="tsf"
                     /locus_tag="PLG69_07750"
     CDS             673..1272
                     /gene="tsf"
                     /locus_tag="PLG69_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPI43436.1"
                     /translation="MAEITATQVKELRDRTGAGMMDCRKALIEKNGNIDEAIKYLREK
                     GISKAEGKANRATKEGIIHSYIHFNNRIGVLLELNCESDFVARTDEFKALADEIAMQI
                     AATNPLAISPEQIDPAILEREKEIAYNKAVNEGKKPEIIEKIVEGNLKKFCNEHSLLG
                     QELISDSTKTVKDLLTNAIATTGENIQIARFVRYQLGGE"
     gene            1323..2051
                     /gene="pyrH"
                     /locus_tag="PLG69_07755"
     CDS             1323..2051
                     /gene="pyrH"
                     /locus_tag="PLG69_07755"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPI43437.1"
                     /translation="MKNVYKSENIHRVLLKLSGEVLSGKKGIIYDETVIDSLTDAIIT
                     VHNQNYELGIVLGGGNIFRGGSWKNKNLNRVVLDSIGMLATIQNSLYMAEILISKGID
                     CAIFSTLAVDKVVDRYTPQLASSALEQGKVCFFSGGTGNPYFTTDTAAVLRAVELQAD
                     IVLKATNVDGLYSADPKKDKNAHFISSASFEECLQKRLGVMDLTAFSLAMDNAMPIKI
                     FNISKPQMLIEALTNAETGTYIHP"
BASE COUNT          766 a          402 c          473 g          541 t
ORIGIN      
        1 gaatatatcc tttttgtagg aaccaaaaaa caggcacagg cagctattaa agaagctgct
       61 gaaaaagccg gtgtctttta tgtaaaccag cgttggtatg gtggaatgct aactaatatg
      121 gctactatcc gtcaaagcat tgaaaagatg aagtattatg aagaaatcgt agctgacggc
      181 actatcaatg gttacaccaa gctggaacaa caaaaaatga aaagaatgca cgataaaatt
      241 gaattttcct taggcggtat ccgcgaaatg gatgccctgc caggatgtat ttttattgtg
      301 gatactgaat atgaaaaaat agccgtgcac gaagcacgta tcttgaatat tccgattgtg
      361 gcaatggtag ataccaattg cgatcccgat ttggttgatt atgtgattcc cagtaacgac
      421 gatgccacgc gtgccattca tctgatttca gatattatgg caaatgccgt tattgaagga
      481 aaaggacttg ccacagaggg agaaagcact gaagaagaaa caactgaaac aactgctgaa
      541 gaagaaacag aggattttga agccgaattg gctgcagcag aagaaaaagt ggaatttgat
      601 gttcccgaat tagcggaaac ggaacaataa aaaactaacc ccaaaactta ctttaaccaa
      661 aaaggagaaa aaatggcaga gattactgca acccaagtaa aagaattgcg tgatagaacc
      721 ggagccggaa tgatggattg ccgcaaagcc cttattgaaa aaaacggaaa tattgatgag
      781 gcaataaaat acctgcggga aaagggaatc agtaaagcag aaggaaaagc aaaccgtgcc
      841 actaaagaag gtatcattca ttcatacata catttcaaca accggattgg agtgcttctg
      901 gaactgaatt gcgaatccga ttttgttgcc agaaccgatg aatttaaagc tttggcagat
      961 gaaatagcaa tgcaaattgc cgctaccaac cctttagcta taagtcccga gcaaattgat
     1021 cccgccattc tggaaagaga aaaagaaatt gcctataaca aagctgtgaa tgaagggaaa
     1081 aaacccgaaa ttatagagaa aattgtggaa ggtaacctga aaaagttctg caacgaacat
     1141 tcccttttag gacaagaact gatcagcgac agcacaaaaa ccgtgaagga cttactgaca
     1201 aacgctatag ccacaacagg cgaaaatatt caaattgcca gatttgtccg ttatcaatta
     1261 ggtggcgaat aaaagctgaa cataaagctg taaaccttcc tgaaacagcc gggagttcaa
     1321 gtatgaaaaa tgtttacaaa agcgaaaata tccatcgggt gctgttgaaa ctttccggag
     1381 aagttctttc cggtaaaaaa ggcataatct acgatgaaac agtaattgac tccctgaccg
     1441 atgccataat tacagttcat aaccaaaatt atgaattagg catcgtttta ggcgggggaa
     1501 atatattccg cggcggttcc tggaaaaata aaaatttgaa cagggtagtt ctggatagta
     1561 tcggtatgct ggcaactatc cagaattccc tgtatatggc agaaatcctt atttccaaag
     1621 gaattgactg tgccattttt tccactctgg ctgtggataa agtagttgat cgctatactc
     1681 ctcaattggc ttcctccgct ttggaacagg gaaaggtctg ttttttcagc ggaggaacag
     1741 gaaatcctta ttttactaca gataccgctg ccgttttacg cgctgttgaa ctgcaagctg
     1801 atattgtgtt aaaagcaact aatgtggatg gactttacag tgctgatccc aaaaaagata
     1861 aaaatgccca ttttataagc agtgccagct ttgaggaatg tttgcaaaaa cgcttgggag
     1921 ttatggattt aaccgctttt tctctggcta tggataatgc tatgccaatt aagattttca
     1981 acatcagtaa gccgcagatg ttaattgaag cacttactaa tgcggaaaca ggaacctata
     2041 ttcacccctg aacatagttt ctgcctggta aagaggatat tatggaaaat ataaaagata
     2101 atactaaaga aaaaatgcag aagagctttg atgcaatgct gcatcaatat tccaaaatca
     2161 gaaccggacg cgccagcgct tc
//
LOCUS       DAOPFL010000183         2174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_71408_length_2174_cov_96.877301, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000183 DAOPFL010000000
VERSION     DAOPFL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71408_length_2174_cov_96.877301"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLG69_07760"
     CDS             complement(<1..875)
                     /locus_tag="PLG69_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43438.1"
                     /translation="MFTRYRIILLLLIVIILSGCEINRLRSAEELYRKNLYAAAIVEL
                     DDLIANAENKAIATRAEIMRSNCYYALAEAAVEKKNWPLAIRFFKLANSDSANLRLAE
                     IYRDLAMEAEKEGNRIVSKSYLDAIIREVPESPLIPEVLYRRIGYFLDVKADNESAWN
                     DYMRLFDNYPNSPYEILARNYVMRFMPGKLKYAEVLTEQGYYADALKELFEISKYPVV
                     DMNQVNKLISDVYEKQAEELLSEQDYIEADRLFRIAMQYYPQKKESIIHRLESITNLF
                     IQKGNSLLEAGDFQGA"
     gene            complement(859..1596)
                     /locus_tag="PLG69_07765"
     CDS             complement(859..1596)
                     /locus_tag="PLG69_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPI43439.1"
                     /translation="MELTILGSGPGLPQLDKHLSSILIRKENNLILADCGDSCAHRLL
                     EHKVAAEELDAIFITHYHPDHLDGLFMVLQMLYLQNRKRDLLLFLPERPASFLEILQL
                     MYTYPQKFGFNLLIRDIEQAELYFDWIYAVPNDHLYRYASIISEYKLPNQMKSWSLCF
                     TGKQGKFVYTSDIETTDCIMDTLKDAHTVLVDAGHPEAEQIIKLKYTAIKKIILTHGI
                     TPELEDRKDELNPEIFEFAREDFVYQI"
     gene            complement(1599..>2174)
                     /locus_tag="PLG69_07770"
     CDS             complement(1599..>2174)
                     /locus_tag="PLG69_07770"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPI43440.1"
                     /translation="FFAFALVNTTQANQEAMALMALIALSFAGSTFGFLPYNFNPARI
                     FLGDAGSMFIGYFLSSMGILMARYAANRYNDMFFYLLPVLLLSYAIFDISLVSFTRRR
                     DGRQITQGGRDHSTHRMNTILGSVKITAIIIYTINVLIALTSIMIFYIGNRHLLIITT
                     VIFALFFLFIGRKLDKVPIVVPANQQKHKKE"
BASE COUNT          693 a          456 c          376 g          649 t
ORIGIN      
        1 gcaccttgaa aatcacctgc ttccagtaaa ctgtttcctt tttggataaa gagattagtg
       61 atggattcca ggcgatgaat aatgctctcc tttttttgag gataatattg cattgcaatt
      121 ctaaacaaac ggtctgcttc tatataatcc tgttcgctaa gcagttcttc tgcctgtttt
      181 tcataaacat ccgaaatcag tttattcacc tggttcatat ctacaaccgg atatttggaa
      241 atttcaaata gctcttttaa ggcatcggca tagtagccct gttctgttaa gacctcagca
      301 tatttcaatt ttcccggcat aaaacgcatt acataatttc gtgccagaat ttcataagga
      361 ctattgggat agttatcaaa aaggcgcata taatcattcc aagctgattc attatctgcc
      421 ttcacatcta aaaaatatcc aattctgcga taaagcactt caggaattaa aggactttcc
      481 ggaacctctc taattatagc atccagatag ctcttgctta ctatccggtt accttctttt
      541 tccgcttcca tagcaaggtc acgataaatt tctgccagac gcaaattggc ggaatccgaa
      601 tttgccagct tgaaaaaacg aattgccagg ggccagttct ttttctctac agctgcttct
      661 gctaaggcat aataacaatt ggaacgcatt atttcagccc gggtggctat agctttattt
      721 tcagcattag caatcagatc atccaattcc acaattgctg cagcgtaaag atttttccgg
      781 tataactctt ccgcacttct taagcggttt atttcacagc cgctaagaat tattactata
      841 agtagcagga gaataattct atatctggta aacaaaatct tctctggcga attcaaatat
      901 ctcaggattc agttcatctt tccggtcttc caattccggg gttattccat gggtgaggat
      961 tattttcttt atggcagtat atttcagttt tattatctgt tccgcttcag gatgacccgc
     1021 atccactaaa acggtatgtg catcttttaa tgtatccata atgcaatcgg tggtctctat
     1081 atcactggta taaacaaatt ttccctgttt tccggtaaaa caaagtgacc aggatttcat
     1141 ctgattgggc agtttatatt cactgattat agatgcatac cgatacaaat gatcattggg
     1201 cacagcataa atccaatcaa aatatagctc tgcctgttct atatcccgaa ttaacaaatt
     1261 aaaaccgaat ttctgagggt aggtatacat caattgtaag atttccagaa aggaagcagg
     1321 acgctccggt aaaaacagca aaagatcgcg ctttctattt tgcaggtata acatttgcaa
     1381 aaccataaac agaccatcaa ggtgatcggg atggtaatgt gtaataaaaa tggcatccag
     1441 ctcttcggca gcaactttat gctccagtaa acggtgtgca cagctatctc cacaatcagc
     1501 cagaattaaa ttattctctt tccgaataag aatactggaa agatgtttat caagttgtgg
     1561 caaacctggt cccgaaccta aaatagtcag ttccatattt actccttttt gtgtttttgc
     1621 tggtttgccg gaaccacaat gggcacctta tccagcttgc gtccaataaa taaaaagaaa
     1681 agtgcaaaaa taacggtcgt tataatcagt aaatgccgat tgccaatgta gaaaatcatt
     1741 attgaagtta acgctatcag cacattgatg gtataaataa taatggcagt aattttaact
     1801 gagcccaaaa tagtattcat cctgtgcgta ctatgatctc ttcctccttg ggtaatttgc
     1861 cttccatccc ttctgcgggt gaaacttacc aaagaaatat caaaaatagc ataagaaagc
     1921 aaaagcaccg gtaataaata gaaaaacata tcattataac gattggcagc ataacgagcc
     1981 atcaaaattc ccattgagga taaaaaataa ccgataaaca tactgcccgc atcacctaaa
     2041 aatatgcgcg ccggattaaa attatagggc agaaaaccaa aagtagaacc ggcaaaacta
     2101 agagcaatca atgccatcaa agccattgct tcctgatttg cctgcgtcgt attgaccaaa
     2161 gcaaaagcaa aaaa
//
LOCUS       DAOPFL010000184         2166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_71818_length_2166_cov_55.684036, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000184 DAOPFL010000000
VERSION     DAOPFL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71818_length_2166_cov_55.684036"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /locus_tag="PLG69_07775"
     CDS             <1..1905
                     /locus_tag="PLG69_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43441.1"
                     /translation="RDAKIRDMIEHWNPVTSPADCNNTIPYDDLPGSTPDEKANAYLN
                     MSDEDPVKMGMRRDVMLWSDDERQATAETAKKLGETGVKAVGDAYGGPYGEWTNEIVN
                     QMLDEDETADDCGTMDIDIPVTASKTLIISKANMPDDDPRITVIMNAPQNLEQQLPPG
                     EYHIIALAEGFIRGVYENLQIVKTSVTNLTAELLNLAENPIIIEDLTVQEGCPTVNQP
                     VHSHLSCVSTIGQELSFAWTVSGGSYTGFNPIGTELTFTPTEEKEYTISVDVSDNLNN
                     HKTRSVSLASLGGMLAIDDWEIGSEDFTDNKLNPGEKITIELFVTNTGTTDLSGYHNI
                     PSGNGFTSLFNSTHVDISAGTTIAIYVPLKINSELSINEITLQYQFTTENQNHNTVLI
                     SDSVELPVDFYVTIDEISDVVTDRIVNITGKIANPSLTTALLILDNDSEHSIEMNLNN
                     GNFSQAVVLNGSTAEVSHTVYVIAVSGGLTAEDTMTFTSLVPIMSLHCTLTWDTSGTD
                     VDFWITDPNKEKCYYAHRHTASGLALDVDDTDGYGPENITSPSVIPGDYTVQVHYYSD
                     HDYENAIGTNCVVVVRQTDLPPINYYGYLADTGDLWTVTTLHYDSEKGWSIKPNNAYS
                     KVNLATLPKK"
BASE COUNT          695 a          376 c          456 g          639 t
ORIGIN      
        1 cgggatgcaa aaattcggga tatgattgaa cactggaatc ctgtaacttc tcctgcagat
       61 tgtaataata ccattcctta tgatgattta cctggttcta ctcctgacga aaaagcaaac
      121 gcctatttga atatgagtga cgaagaccct gtaaaaatgg gtatgcgaag agatgttatg
      181 ctttggagtg atgacgaacg ccaagctaca gccgaaacag ccaaaaaact tggcgaaacg
      241 ggtgtaaaag ctgttggtga tgcttatgga ggaccttatg gtgaatggac aaatgaaatt
      301 gtgaaccaaa tgctggatga agatgaaaca gctgatgact gcggcactat ggatattgat
      361 attcctgtta cggcaagtaa aacacttatt atttccaaag ctaatatgcc tgatgatgac
      421 ccccgaatta cagttataat gaatgctccc caaaatctgg aacagcaatt acctcccggt
      481 gaatatcata ttattgcttt agcggaaggt tttatacggg gtgtgtatga aaatctgcaa
      541 attgtcaaaa cttcagttac caatcttaca gcagaattat taaatttagc agaaaacccc
      601 attatcattg aggatttaac tgttcaagaa ggttgtccaa cggtaaatca acctgttcat
      661 tcacatctat cctgcgtaag cacaatcggg caagagcttt cttttgcgtg gactgtttcc
      721 ggaggttctt atacagggtt taatcctatt ggaacagaac tgacttttac ccctactgaa
      781 gaaaaggaat atacgatttc ggtagatgtt tcggataatc tcaataatca taaaacaaga
      841 agtgtttccc ttgcctcctt aggaggaatg ttagctattg acgattggga aattggtagt
      901 gaagatttca cagataataa gctgaatcct ggcgaaaaaa taactattga gctttttgtt
      961 actaatacag gaacaactga ccttagcggt tatcataata ttccctccgg aaacggtttt
     1021 acctctttat ttaattcaac tcatgttgat atatctgctg gaacaacgat agctatatat
     1081 gtccctctta aaattaacag tgaacttagt ataaatgaaa taactctgca gtatcaattt
     1141 acaactgaga atcaaaacca taacaccgtc ctgattagtg attctgtaga actaccagta
     1201 gatttttatg tgacgataga tgaaattagt gatgtagtaa ctgaccgtat tgtgaatatt
     1261 acaggaaaaa tagcaaatcc cagcttaact accgcactat tgatattgga taacgatagt
     1321 gaacattcca ttgaaatgaa cttgaataac ggaaattttt cccaggcagt ggttctgaat
     1381 ggttcaacag cagaagtttc acatacggtt tatgtgattg ccgtttccgg aggtttaacc
     1441 gctgaagata caatgacttt tacttctctg gtacccataa tgtctttgca ttgtactctc
     1501 acctgggata cttcaggaac ggatgttgat ttctggatta ccgaccccaa caaagaaaaa
     1561 tgctattacg ctcatagaca tacagccagc ggtttagctc ttgatgtaga cgatactgat
     1621 ggctatggtc ctgaaaatat cacttcgcca agcgtaattc ccggtgacta caccgtgcag
     1681 gttcattatt atagcgacca cgattatgaa aatgctatcg gaaccaattg tgtagtagtt
     1741 gttcgtcaaa cagaccttcc accaataaac tattatggtt atttagctga taccggagat
     1801 ttatggactg taactaccct gcattatgac agcgaaaagg gttggagtat taaacccaat
     1861 aatgcatact ctaaagttaa tcttgccact ttacctaaaa agtaggaggt tttttcccgc
     1921 agattacaca gatttcacgc agatttcgct gattacgcag atttgattat ggaataggta
     1981 taggatgaac aggattggaa ggatttatgg gatttaatag gtatgggata aacaggattg
     2041 gaaggattta tgggatttaa taggtatagg atgaacagga ttggaaggat ttatgggatt
     2101 taataggtat gggatgaaca ggattggaag gatttatggg atttaatagg tatgggataa
     2161 acagga
//
LOCUS       DAOPFL010000185         2165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_71860_length_2165_cov_117.138389, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000185 DAOPFL010000000
VERSION     DAOPFL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71860_length_2165_cov_117.138389"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="dxr"
                     /locus_tag="PLG69_07780"
     CDS             complement(<1..974)
                     /gene="dxr"
                     /locus_tag="PLG69_07780"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPI43442.1"
                     /translation="MVKKSIALLGATGSIGTSALAVAEEQNVSIVLATAQKNYSSLLQ
                     KAYQHHIPCLIFTGIEDKELQTKLKSENPDKRIYFGEAELLKAIANENYDIALNAIAG
                     SAGLRYSYAILQNNKTLALANKESLIMGGHILTKMLAGKPILPVDSELSALFQAIGNH
                     PAEEIRFLHLTASGGIFRDLPFEDFNQISPQQALKNPNWTMGTKVTLDSATMFNKALE
                     AMETHWLFNQPYSKIKAVIHPQSIIHSLVEFIDGSFLAQMSMPDMKLPILYALSYPQR
                     VQSQLVETDLMSLPPLTFQEIDPQRYPLFYLGLEAAKAGGIYPTVINSA"
     gene            complement(968..1912)
                     /locus_tag="PLG69_07785"
     CDS             complement(968..1912)
                     /locus_tag="PLG69_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43443.1"
                     /translation="MKIQVLVICLLLITIPLTATKYAGEIFAVSPGVLSTAMGSTGLT
                     YVGSLSAGWWNPALLALNPESGIELMRCEHFEGLMAQNQLSLIIGSKTKTSFTFNHLA
                     IDKIKLTKLENPDDSLSNENRPVVWKTISNQDFILYAGLARQLKKTLYLGISPKLAYR
                     SLAENSGYAFGADLGMFWQINKQANLGVNLRDFFSTQVLWEGGENETVIPNLDLELSY
                     DFSPIKNIPVHLAVRSEILLENREATVETGDLSADFHAGIAVQPISNLCLLAGYDIDC
                     FTAGIGVSYKRLGLNYAFRNGSEDDLGYSQRISVSYQW"
     gene            complement(1961..2036)
                     /locus_tag="PLG69_07790"
     tRNA            complement(1961..2036)
                     /locus_tag="PLG69_07790"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2001..2003),aa:Ile,seq:cat)
BASE COUNT          650 a          463 c          411 g          641 t
ORIGIN      
        1 gcggaattga taacggtggg ataaattcct cccgctttag ctgcttccaa acctaaataa
       61 aataaaggat agcgttgcgg gtcaatttcc tgaaaggtta aaggtggaag agacattaaa
      121 tccgtttcca caagctgcga ttgcaccctt tgaggatagg aaagagcata caaaatcggc
      181 aatttcatat ccggcatact catttgagct aaaaaagaac cgtcaataaa ttccaccaga
      241 gaatgaataa ttgattgcgg atggattact gccttgattt ttgaataggg ctgattgaat
      301 aaccaatgtg tttccatcgc ttccagtgct ttattaaaca tcgttgccga atccaaagta
      361 actttagttc ccattgtcca gttcggattt tttagtgcct gctgaggtga tatttggtta
      421 aaatcctcaa aaggcaggtc gcggaaaata cctccggaag cagtaaggtg caaaaatctg
      481 atttcttctg caggatgatt tccaattgcc tgaaaaagag cgctaagttc gctatctaca
      541 ggcaaaattg gcttgcccgc aagcattttg gttaaaatat gtcctcccat aattaaggat
      601 tctttatttg ccaaagccag tgttttattg ttttgtaaga tagcataact atagcggagt
      661 cctgccgaac cggctattgc atttaaagca atatcgtaat tctcattcgc aattgccttt
      721 agcaattctg cttcgccaaa atatatcctt ttgtcaggat tttcactttt cagcttggtt
      781 tgcagttcct tatcttctat tcctgtaaaa atcagacagg gaatatgatg ttgataagct
      841 ttttgcagaa gggaagaata atttttctga gccgtagcca aaacaattga aacattctgc
      901 tcttcagcca ctgctaaagc ggaggttcca attgaacctg ttgctcctaa aagagcaatt
      961 gatttcttta ccattgataa gaaacagaaa tcctttgaga atagcccaaa tcgtcctccg
     1021 aaccattacg gaaggcataa tttaacccta agcgcttata ggaaaccccg attccggcag
     1081 tgaaacaatc aatatcgtat ccggcaagta aacacaaatt ggaaattggc tgaactgcaa
     1141 ttccggcgtg gaaatcggcg cttaaatcac ccgtttccac ggttgcttct ctgttttcca
     1201 gtaaaatttc actccgcacg gcaagatgaa caggaatatt ctttatggga gaaaaatcat
     1261 agctcaattc caaatccaga ttgggaataa cagtttcatt ctctccccct tcccaaagaa
     1321 cttgagtgct gaaaaaatct ctgaggttta cccctaaatt tgcctgttta tttatctgcc
     1381 aaaacattcc cagatcagct ccaaaagcat aaccgctgtt ctctgccaaa gacctgtatg
     1441 ccagcttggg tgaaattccc aaatagagtg ttttcttaag ctgtctcgcc aaacctgcat
     1501 aaagaataaa gtcctgattg ctaatggttt tccaaacgac aggacggttt tcgttactta
     1561 aactgtcatc ggggttttcc agtttggtta attttatttt atctatggct aagtgattga
     1621 aagtaaaaga tgttttggtt ttggagccaa taattaaact tagttgattt tgcgccatta
     1681 aaccctcaaa atgctcacag cgcataagtt ctattccact ttcaggattc aaagctaaaa
     1741 gagccggatt ccaccaacct gcagataaac tgccgacata agtaagccct gtagaaccca
     1801 ttgctgtgga aagaactcca ggacttacag cgaaaatttc tcccgcatat tttgtagcag
     1861 taaggggtat agttatcagc aacaagcaga taaccagaac ctggattttc atttaacctt
     1921 cctcttgtct gttcatactt ataaaaaacg ggagaaaaaa tggtgggccc agagggattc
     1981 gaacccccga ccaaccggtt atgagccggt agctctacca gctgagctat aggcccccta
     2041 agttttttat tacattttcc cagcttgcct aattccgttt cggaaaatac caaatctata
     2101 cttataataa ctgattttat cagctttggc aagtcggaat tccctgccct gatttacatt
     2161 taaaa
//
LOCUS       DAOPFL010000186         2158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_72183_length_2158_cov_91.262007, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000186 DAOPFL010000000
VERSION     DAOPFL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72183_length_2158_cov_91.262007"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            596..799
                     /locus_tag="PLG69_07795"
     CDS             596..799
                     /locus_tag="PLG69_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPI43444.1"
                     /translation="MPKMTVDPNYCKGCGLCIIACPRKIIRLSENINAKGYNYAECIE
                     QEKCTACKMCYITCPDVAITIEK"
     gene            808..1887
                     /locus_tag="PLG69_07800"
     CDS             808..1887
                     /locus_tag="PLG69_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPI43445.1"
                     /translation="MSKILMKGNEAIAEAAIRSGCRLYFAYPITPQSELIEYMAKMMP
                     KVNGTFIQAESEVAAINMVYGAAGSGKRVMTSSSSPGISLKMEGLSYIAGAELPCVVV
                     NVQRGGPGLGDIQPAQGDYFQATKGGGHGDYRLIVLAPSSVQEFADMASEAFDLADKY
                     RNPVMILADGMLGQMMEPVEFKPAKTEEELQELANQHLSWCICPNEDGDKKHHHEINS
                     LELDPLVLEQHVLGLCKKYAEIQKNEIRYETYNISDENEVLCVAWGTASRVVKSAINE
                     VLKEGKSVGLIRPIRVWPYPYEAIKQAIGKNVKKVYVFELNSGQMVEDVKIAVEGKVP
                     VDFWGKVGGVVFTPAEIKEKLEECF"
     gene            1903..>2158
                     /locus_tag="PLG69_07805"
     CDS             1903..>2158
                     /locus_tag="PLG69_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HPI43446.1"
                     /translation="MEIIAKRPEALTKVPFHYCPGCMHGVAHRLIAEAIDENGLINQI
                     AGVAPVGCAVFAYNYFNCDMAEAAHGRAPAVATGIKRARPD"
BASE COUNT          692 a          381 c          472 g          613 t
ORIGIN      
        1 tttgagcaat taccggcgca ataaacatta cctgaatccc aattaggcaa gccaaaaatt
       61 tctttttgtg tttcttcatc attttcctta gctcatgtga tgggagggat aatttttaga
      121 gccgggcata ttgtcaagga aaaattttcg ctatagggat aatccatcta tattacaata
      181 tgttatacaa attgctactt attccattct atttcccgta acgaccaggt aacaaccaag
      241 taacaattcc gtaacgagac aacggaagtg ttacggaagt gttacggagt tgttacttaa
      301 tcaactatgc tccaaacaat tacagctctc aaacctaatc aggcaaattc gtttcccgca
      361 gatttcgcat attttaccct aattaagcag atttgatttt tttatgccgt aatgagtcat
      421 attacgctgt aaaaagaatc gtaaagaaca gggttgacac agcgcaaatg actcttaaca
      481 tagaacgacg gggatgtcgt tccctcggtt ttataaagaa ataatttgac agaataaggc
      541 aaaaaaatgg tttatcacag aaaggaaaaa tataaaaaaa agagaggtgt ttgttatgcc
      601 taaaatgact gttgacccca actattgcaa aggttgtggc ttgtgtatta ttgcttgtcc
      661 gagaaagatt attcgtttat cggaaaacat taatgccaag ggctacaact atgcagagtg
      721 tattgagcag gaaaagtgta ctgcctgcaa aatgtgttat ataacctgtc ctgatgtagc
      781 aataacaatt gagaagtgag gttatgaatg tctaaaattt taatgaaagg aaatgaggca
      841 attgcagaag ccgcaattcg tagtggttgt cgcttgtatt ttgcctatcc catcaccccc
      901 caaagcgaat taattgaata tatggcaaaa atgatgccta aagtaaatgg cacttttatt
      961 caagcggaaa gcgaagttgc tgccataaat atggtttatg gagctgctgg aagcggaaaa
     1021 agagtgatga catcttcttc ctctccgggt atttcgttaa aaatggaagg tctatcctat
     1081 attgcgggag cggaacttcc ctgtgtagtt gttaatgttc aacgcggagg tcccggttta
     1141 ggagatatcc aacccgcaca aggtgattat tttcaagcaa ccaaaggtgg aggtcatggc
     1201 gattatcgtt taatcgtttt agcaccgagt tcggttcagg aatttgcaga tatggcaagc
     1261 gaagcatttg atctggcaga taaatatcgc aatcctgtta tgattcttgc cgatggaatg
     1321 ctgggacaaa tgatggaacc cgttgagttc aaacccgcaa aaacggagga agaactgcaa
     1381 gagcttgcca atcaacatct ttcctggtgt atttgcccca atgaagatgg cgataaaaaa
     1441 caccatcatg aaataaattc tctggaactt gaccccctgg ttttggaaca gcatgttttg
     1501 ggtttatgta aaaaatatgc ggaaatccaa aagaatgaaa ttcgctatga aacctataat
     1561 atttcagatg aaaatgaagt tttatgcgtt gcctggggaa ctgccagccg agttgtaaag
     1621 tcagctatca atgaagttct aaaagaagga aaaagcgttg gcttaattcg tcccattcgt
     1681 gtatggccct acccttatga agcaatcaaa caagcaattg gtaaaaatgt gaaaaaggtc
     1741 tatgtttttg aactcaattc aggacaaatg gtagaagatg taaaaattgc tgtagaagga
     1801 aaggttccgg ttgatttttg gggaaaagtg ggaggtgttg ttttcacccc tgctgaaatc
     1861 aaagaaaaac tggaagaatg tttttaaagg aaggaggaaa aaatggaaat tattgcaaaa
     1921 cgccctgaag cacttactaa agtccctttc cattactgcc ctggatgtat gcatggagta
     1981 gctcatcgtt taattgctga ggcaattgat gaaaatggtt taatcaatca aatagccggt
     2041 gtggcacctg tcggatgtgc cgtttttgcc tataactatt ttaattgtga tatggcagaa
     2101 gcagctcatg gacgcgctcc tgccgttgct acaggcataa aaagagcccg tcccgata
//
LOCUS       DAOPFL010000187         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_72565_length_2150_cov_104.794749, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000187 DAOPFL010000000
VERSION     DAOPFL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72565_length_2150_cov_104.794749"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLG69_07810"
     CDS             <1..238
                     /locus_tag="PLG69_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43447.1"
                     /translation="LKPNNTATGKRVGKFFLCCSNKRFLKEFILLLSALCSQRYVTLT
                     GFCKKERKKENNRGFHFYEGLTPPSLSLCRSPSF"
     gene            667..1080
                     /locus_tag="PLG69_07815"
     CDS             667..1080
                     /locus_tag="PLG69_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HPI43448.1"
                     /translation="MKKGFPFVVIVLSAILGIGSRKYGNYLPNFIAEYTGDTMWALAF
                     YALFRMLFSRKRNWEIALLTFDFSCLIELSQLWHPFWLEKIRSTIIGGLLLGFGFRYT
                     DLFCYFAGCLLGFLIFSLGKSRRSNKKLFGETTCL"
     gene            1186..2061
                     /gene="dapA"
                     /locus_tag="PLG69_07820"
     CDS             1186..2061
                     /gene="dapA"
                     /locus_tag="PLG69_07820"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPI43449.1"
                     /translation="MLQGTYVALITPFKNGSIDWTALEDLIQFHLDNGTTGILLLGTT
                     AETSALASDEKDALLRFALKKIDGKLPVIIGTGTNNLHQTLASTQKAKELGADYALVI
                     TPYYIKPTQKGMLEYFGIIASKIDIPIVIYNVPGRTGVNITAATTVELAKTYPNIVGI
                     KDASGNLVQVTQIVRDAPENFSVMCGEDALNLPLIAIGAKGTISVTANVAPKLMSEHI
                     ATCLKGDFITAAKQHQYLARLNEMMFIETNPIPAKEALAMMGKITLAFRSPMCPLMEN
                     NRALLKQCLQDYKLI"
BASE COUNT          634 a          397 c          417 g          702 t
ORIGIN      
        1 cttgaagcca aataatacag ctactggaaa aagggttggc aaattctttc tttgctgcag
       61 caataaaagg tttctaaagg aatttatact tttgctctct gccctctgct ctcagcgcta
      121 tgtcaccctg acgggctttt gtaaaaagga aaggaaaaaa gaaaataatc ggggctttca
      181 tttttacgag gggcttacgc caccatcgct atcattgtgt cgctctccga gcttttaatg
      241 tatctcaacc ttctaaaccc tctaaaccct ctcaaccttt tttttatgag gggcttacgc
      301 caccttttac atcattgtgt cgccctgacg ggcttttgta aaaaggaaag gaaaaaagaa
      361 aaaaaactcg gggctttcat ttttacgagg ggcttacgcc accatcgcta tcattgtttc
      421 gctctccgag cttttaatgc atctcgaccc tctcaacctt ctcaaccttt tttagttctc
      481 aattactctc tgttttcctt ctaaatttca ttcttcattt ccctattggc ttctgctctt
      541 ttagcaagct ccgtctacaa agtgcttgac ttaaaatctt ttgtatagat tatggtaaag
      601 atattatttg agattagata aagaaaaaag gcaatacagc aggtggatag aaaaaagcca
      661 gagattatga aaaagggatt tccttttgtg gttattgtgt taagcgcaat tttgggaatt
      721 ggttcgcgta aatatggaaa ttatctaccg aattttattg cagaatatac cggtgataca
      781 atgtgggcat tagcttttta tgctttattc cggatgcttt tttcccgtaa aaggaattgg
      841 gagattgctt tgttaacctt tgatttttcc tgcttaattg aacttagcca attgtggcat
      901 cctttctggc tggaaaaaat ccgttccact attatcggtg gtttactttt aggttttggt
      961 tttcgttata ctgacctttt ttgctatttt gctggttgtc ttttgggttt tcttatattt
     1021 tctttaggta aaagcaggag aagcaataaa aagctatttg gggagacaac ttgcttataa
     1081 aaataacctt ttcatctttt ttgattgacg aaaatcgtct cttttgctat tatggaataa
     1141 aatatgttta tagataagga tatataaccc tggaggttaa aaaaaatgct gcaaggaact
     1201 tatgttgccc tcattacacc ctttaaaaat ggcagtattg actggactgc tttagaagac
     1261 ctgattcagt ttcatctgga taatggaaca acaggtattt tacttttagg aacaactgct
     1321 gaaacaagcg ctctggcttc cgatgaaaaa gatgctcttt tgcgttttgc tctgaaaaaa
     1381 attgacggaa agttacctgt aataattggc accggaacaa acaatctaca tcagacactg
     1441 gcttccacgc aaaaagcaaa agaattagga gctgattatg ctttggttat tactccctat
     1501 tacatcaagc ccacgcaaaa aggtatgttg gaatactttg gcatcattgc ttccaaaatt
     1561 gatattccca ttgtgattta taatgtcccc ggtagaacag gagtgaatat tactgctgct
     1621 accactgttg aattggcaaa gacctatcct aatattgtcg gaattaaaga tgccagtgga
     1681 aatttagtgc aggtaacgca aattgtgcgt gatgctccgg aaaatttttc cgtgatgtgc
     1741 ggtgaagatg ctttgaacct tcctttaatt gctattgggg ctaaaggaac tatttctgtt
     1801 actgctaatg tagctccaaa actgatgagt gaacatattg ccacctgttt gaagggtgat
     1861 tttattacag cagccaaaca gcatcaatat ttggctcgct taaatgaaat gatgtttatt
     1921 gaaaccaatc ctattcccgc gaaagaggca ctggcaatga tgggaaaaat tactttggca
     1981 tttcgcagtc cgatgtgtcc tctaatggaa aacaatcgtg ctctgctaaa acaatgtctg
     2041 caggactata aactgatata agaggtaata aatgacacaa aaaaagttgc tctatggctt
     2101 ccagctgatt gaaaagaaag aaattaaaga gataaaggta actgcctatc
//
LOCUS       DAOPFL010000188         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_72566_length_2150_cov_91.259189, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000188 DAOPFL010000000
VERSION     DAOPFL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72566_length_2150_cov_91.259189"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>2150
                     /locus_tag="PLG69_07825"
     CDS             34..>2150
                     /locus_tag="PLG69_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPI43450.1"
                     /translation="MKQVFIIMLLLITCLCPLWGMRKALVIGNSNYNEQVLRNSINDA
                     SDVYNALKGLGFSASLFTDLNREGFFRAVGEFVSTLTPSDEVVFFYSGHAAQIEGKNY
                     LIPVNEYIDSATRCAYLSYDTSMLLDELSRAAISIVILDACRDNPFAFSRSIRSGLAP
                     MTVSAGSQCVIFATEQGRTASDGEGRNSPFTESLLANITTPGIKITDLIQRVSNEVAI
                     KTQERQIPYSTGTLRQDFYFVKAEPVTPISPVIPPSIPQEPKPVVSPSYGSIKVKTSF
                     GGDLYIDGEFSQSLFAGDQSIIKDIAVGVYELKLVSPKETIIRLANVNKNQTTMIDFD
                     APDSYREEPKKIPEQQIIIPEQQIPHSEKQSIELNSLTPLSPITKKVSVGTGALTIES
                     PLAGDLYLGGDFIGKLTAKTSKKFSNVQAGSYTLEVMHQNSFCRKEIRIYNKDNLKLT
                     LSQTEFSKYPDGFQYVSGGTFQMGTQFLNRSEDERPRHEVQILPFLMKKTEVEQKLWT
                     DVMGYNPSEIKGDSYPVTNVSFYEAVQFCNALSTLNGLTPCYKIDKSKTDPTNLCNLD
                     TLRYIISCNWNASGYRLPTEAEWEYVSRCGDNYNKLQFSGSNNANDVAWNSKNSENKI
                     HPVATKNPNQWGFYDLSGNVFEWCWDYYDIYKKDKQVHPKGAEKGVYRIARGGSFNSD
                     IDACSSTARGGFAPNFKGNNLGFR"
BASE COUNT          652 a          406 c          427 g          665 t
ORIGIN      
        1 tatagcaaaa ccactttatc taaggaggtg ggtgtgaaac aggtgtttat tattatgctt
       61 ctgcttatta cttgtttatg ccctttatgg ggtatgcgca aagcattagt aattggaaat
      121 tccaattaca atgagcaggt tttgcgcaat tctataaatg atgcttcaga tgtgtataat
      181 gctcttaaag ggttagggtt tagcgcttct ttatttactg acctcaatcg ggaaggtttt
      241 tttagggcag ttggtgaatt tgtttctaca cttactcctt ccgatgaagt ggtctttttt
      301 tatagtggcc acgctgccca aattgaaggt aagaattatc ttattccggt gaatgaatat
      361 attgattcag caacgcgttg tgcttatctt tcttatgata cttcaatgct tctggatgaa
      421 ttgagcagag ctgctataag cattgtaatt ttagatgcct gccgagataa tccttttgct
      481 ttttcgcgtt ccattagaag cggtttagcc ccaatgacag tttcagccgg ctctcaatgt
      541 gtaatttttg ctacggaaca aggaagaact gcctctgatg gagaaggtag aaatagtcct
      601 ttcacggaat ccttgcttgc caatattacc actccgggga ttaaaattac tgatcttatt
      661 caaagggtca gtaacgaagt agcgattaaa acccaagaac ggcaaattcc ctatagcaca
      721 ggaactttaa ggcaggattt ctattttgtt aaggcagaac ctgttacacc catttctcct
      781 gttattcctc cttctattcc tcaagagccc aaacctgttg tttctccaag ttatggcagc
      841 ataaaagtta aaacctcttt tggcggggac ttgtatatag atggtgaatt cagccaaagt
      901 ttgtttgcag gagaccagtc cattatcaaa gatatagcgg ttggagttta tgaactgaaa
      961 ttggttagtc cgaaggaaac aattatccgt ttagctaatg ttaataaaaa tcagaccaca
     1021 atgattgatt ttgatgctcc cgattcttat agagaagaac ctaaaaagat accggagcag
     1081 caaataatta ttcccgaaca gcaaattccg cattccgaaa aacaatctat agagctcaat
     1141 tccttaactc cactatcacc tataaccaaa aaagtgtcag tgggcacagg tgctttaacc
     1201 atagaaagtc ctcttgctgg agacctttat ttgggaggtg attttattgg caaactgaca
     1261 gcaaaaacct ctaaaaagtt ctctaatgtc caagctggtt cttacacttt ggaagtaatg
     1321 caccaaaatt ctttctgtcg taaggaaatt agaatttata acaaggataa cctaaagtta
     1381 actctttccc aaactgagtt ttccaagtat cctgatggtt tccaatatgt ttccggcgga
     1441 acttttcaga tgggaactca atttttaaat cgcagtgaag atgaacgtcc tcgtcatgaa
     1501 gtgcaaatcc ttcctttcct gatgaaaaaa acggaagtgg aacaaaaatt atggactgat
     1561 gtaatgggct ataacccttc ggaaattaaa ggcgatagtt atcctgttac caatgtttcc
     1621 ttttatgaag cagtgcaatt ttgcaatgct ctaagtactt taaacgggtt aactccttgt
     1681 tataaaatag ataaatccaa aactgatcct accaatctct gcaatttaga tactttgcgt
     1741 tatatcattt cctgcaactg gaatgcctca ggttaccgtt tacctactga agcagaatgg
     1801 gaatatgtat ctcgttgcgg agataactat aacaagcttc aattttccgg aagcaacaat
     1861 gccaacgatg tcgcctggaa tagtaaaaac agcgaaaaca aaatacatcc ggtggccact
     1921 aaaaatccaa atcaatgggg cttttatgac ctcagtggta atgtatttga atggtgttgg
     1981 gattattatg acatctacaa aaaagataaa caggttcatc ccaaaggagc ggaaaaagga
     2041 gtttatcgca ttgccagagg cggaagtttt aatagtgata tagatgcctg ttcttctact
     2101 gcccgaggtg gttttgctcc caattttaaa ggaaacaatc tgggctttcg
//
LOCUS       DAOPFL010000189         2125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_73727_length_2125_cov_101.800483, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000189 DAOPFL010000000
VERSION     DAOPFL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73727_length_2125_cov_101.800483"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLG69_07830"
     CDS             complement(<1..325)
                     /locus_tag="PLG69_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43451.1"
                     /translation="MKRIYIFILITKCVSFLFSYQVIENNFSPDNSTKKRYEIVDYSE
                     DFESGAPGWTHYDAAVSPNNWHIYNFGGTQGNVWWMGDPALAQGSNIGGYYNHQYLVL
                     DTPART"
     gene            981..>2125
                     /locus_tag="PLG69_07835"
     CDS             981..>2125
                     /locus_tag="PLG69_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPI43452.1"
                     /translation="MNKAFCEKLSHLSIEGTAEEIVHRFIEHLKFDLGKDEFSATPYD
                     CYVSFASAVKDILMDRWLITQPQEYALQRKRVYYLSLEYLIGRSLGNAILNLDIQKQS
                     IKALTENGFDLSLLSELEWDAGLGNGGLGRLAACFLDSMATLKIPAYGYGIRYEYGIF
                     FQHIINGEQVETPDNWLRYGSVWEIARPARLFPVYFGGIVKEEILEDGSKKMRWIPEE
                     TVMAMAYDYLVPGFQNNYVNTLRLWSAKSTREFNLAYFNEGDYMQAVADKNHSEMIAK
                     VLYPNDNKMQGKELRFRQEYFFVSATLQDILRRFLKKESDLRKLPEKAAIQLNDTHPA
                     IAVAELMRILVDERNLPWEIAWEITQKCIAFTNHTILPEALEKWQVLL"
BASE COUNT          660 a          355 c          399 g          711 t
ORIGIN      
        1 aagttcttgc cggggtatcc aaaacaaggt attgatgatt ataataacca ccaatattac
       61 ttccttgagc cagagcaggg tctcccatcc accagacatt tccctgggtt ccgccaaaat
      121 tgtaaatatg ccaattatta ggtgaaaccg cagcgtcgta atgagtccag ccaggagccc
      181 cggattcaaa atcctcgcta taatccacta tttcatatct tttctttgtg gaattatcag
      241 gtgaaaaatt gttctcaata acctgataag aaaataagaa acttacacac ttagttatta
      301 agatgaatat ataaattcgc ttcattctct cctccttctg gaaataaagc taaattagct
      361 gttattattt gtaaagaata taacaggtcg tatggtaacc aaagtaaaga taatttatga
      421 gtgtattggt agggatattt gatttctgca aagacaaaag accactcagc tacagcgaac
      481 cataaaaaga taaaaattat aaccaacatt tgaaaaatca tctgctttgc ctacaaaaaa
      541 ccattcttgc tttttgtgct tttttgcacc aggtaagaaa cgctcatcat agtagaacta
      601 actgatttga atgcggaata aatattaaaa atgatcattg ttcctctcaa ctatgaaata
      661 tatctattgt tttcatctat ataaaatcaa aatgaaagta atttgtttca gttgcatttg
      721 aagatggaaa cgattttttt tcttctttca tattcctcat tatatctcct taattgcaat
      781 tagaaatcat tccttgcagg cagaattttt aatttcttca attcactgaa tataatataa
      841 accaattctc tgagttgcca acccattttt tactaactgt attttttagg ttaatatagc
      901 tattctacag ttctatttgc caaaattgtt aaattgctta ttttgtctta taagctttga
      961 cattagagga ggaaaaaatt atgaataagg cattttgcga aaagctttct catcttagta
     1021 ttgagggaac tgctgaagaa atagtgcatc gttttataga gcatctcaaa tttgatttgg
     1081 gcaaagacga attttcagca actccttatg attgttatgt tagttttgcc tcggcagtta
     1141 aggatattct gatggatcgt tggctcataa ctcaacccca ggaatatgct ctgcaacgga
     1201 aaagggttta ttatttatcg ctggaatatt taattggtcg ctctttaggc aatgccattc
     1261 tcaatcttga tattcaaaag caaagcatta aagcacttac cgaaaacgga tttgatcttt
     1321 ctttgcttag cgaattggaa tgggatgccg gtttgggaaa tggaggtttg ggtcgtcttg
     1381 ctgcctgttt tttagattct atggcaactt tgaaaattcc tgcttatggc tatggtatcc
     1441 gttatgaata tggtattttc tttcaacata ttattaatgg tgaacaagtt gaaacacccg
     1501 ataactggtt gcgttatggt tctgtttggg aaattgcgcg tcctgctcgt ctttttcctg
     1561 tttattttgg tggcatagtg aaagaagaaa ttcttgaaga tggcagtaaa aaaatgcgtt
     1621 ggattcctga agaaacggta atggctatgg cttatgacta tttagtgcct gggtttcaaa
     1681 acaactatgt gaatacctta cgcttatgga gcgctaaaag tacgcgtgaa tttaatttgg
     1741 catattttaa tgaaggtgat tatatgcaag cagtggcaga caagaatcat agcgaaatga
     1801 ttgccaaagt gctctatcct aatgataaca aaatgcaggg taaggaatta cggtttaggc
     1861 aggagtattt ttttgtcagc gctactttgc aggatatttt acgccggttt ctaaaaaagg
     1921 aaagtgactt gcgtaaactt cctgaaaaag ctgccattca gcttaatgat actcatcctg
     1981 ctattgctgt tgctgaattg atgagaattt tagtggatga aagaaacctg ccctgggaaa
     2041 ttgcctggga aattactcaa aaatgtatag cttttacgaa tcatacaatt ttgcctgaag
     2101 cgctggaaaa atggcaggtt cttct
//
LOCUS       DAOPFL010000190         2104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_74792_length_2104_cov_81.120547, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000190 DAOPFL010000000
VERSION     DAOPFL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74792_length_2104_cov_81.120547"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..758)
                     /locus_tag="PLG69_07840"
     CDS             complement(318..758)
                     /locus_tag="PLG69_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPI43453.1"
                     /translation="MREKIFSNIVGIQREMLKLLGEVSALTNSPLAIENAIDDVWHPK
                     CDVFQTDTEWIVIAELAGVEKDEISVSISPEYLRISGNRGFPNSNTTVCYYNMEIETG
                     RFDRKIFFPDLNIDKDNPKITYINGILRIAFNLAPIVERIIPVQ"
     gene            complement(778..>2104)
                     /locus_tag="PLG69_07845"
     CDS             complement(778..>2104)
                     /locus_tag="PLG69_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPI43454.1"
                     /translation="FLTVIILLLFALPLLAEDWLTIYNNDLSLVRSRFELELENGRQD
                     INFSDITSRIDPSSVIVTGNGIRIAEQNFEYDLAGKWQIMAKYLDREVLIITKDQSRL
                     TGILKFYSDNSIGIIEKGTERLLVISESETQWIQLAELPDNFYTKPTLHWSIIASKKA
                     KYPVQMSYLTGGLSWDVTYNTVWDEKTLQFNSWVTINNTSGKAFNDVMLKLIAGDINR
                     VYSGYYGKRGSLISYDAGMAPESAAPSFEEKAFHDFHMYTLDQKVSFANNQTKQLELY
                     PPKNIKAYAEYQYPTYATNVKSIIKFKNTVDNGAGMPLPKGIIKVYKQDSDGNLEFIG
                     EDSINHTSRNEEVSINTGTAFDLIASTRLKEQTQISKYITERLIQITLRNNSNKNKTI
                     KVTHQLSPNTRIVEADYKYTIDNNDKVTFSIDIAPDKEIVWTFRERSEY"
BASE COUNT          620 a          389 c          412 g          683 t
ORIGIN      
        1 aagttttgtt ttaaccctaa accggacttt acagcggaaa tttttttaca gagaaatgat
       61 gctattgttg cttcaaaata tagttgcaaa cagaaaataa accggtttaa ggtaaaaaca
      121 aaacagagag aggaatcggt ttaaggttaa aacaagaatc tatattctcc tgcaagccac
      181 tcatcgtttt ctccttacaa ttcacccatc tacaacccat cctttactat gggttgagga
      241 tgagtggctg atgagtggag gttaagttac aagtatgcaa ccggcaacaa acagagaagg
      301 taaggaaaaa ttagttgtta ttgcacggga ataatccttt ccacaatggg tgccaggtta
      361 aaggcaattc ttagaattcc gttgatatag gtaattttag ggttatcctt atcaatgttc
      421 aaatccggaa agaatatctt ccggtcaaac cttccggttt caatctccat attatagtag
      481 caaacggttg tattggaatt ggggaaaccc ctatttccgc taattctgag gtattcagga
      541 gaaatggaaa cgcttatttc atccttttcc actccagcca gttcggcaat cacaatccac
      601 tctgtatctg tttgaaagac atcacatttg ggatgccaaa catcgtctat agcattttca
      661 atggctaagg gactattagt taaggcagaa acctctccca gcaatttaag catttctcgt
      721 tgaattccaa cgatgttgga aaatattttt tcacgcatag caaaactccg tttttgttta
      781 atactcgctg cgttctctaa aagtccatac tatttcttta tcgggagcga tatcaatgct
      841 gaaagtaact ttatcgttat tatctatagt gtatttataa tcggcttcca cgatgcgggt
      901 attaggagaa agttggtggg ttactttaat ggttttgttt ttattagaat tattccttaa
      961 agtgatttgg atgagacgtt cggtgatata tttggagatt tgtgtttgtt ctttcaaacg
     1021 ggtagaggca attaggtcaa aagcagttcc ggtattgata gagacctctt catttctgga
     1081 agtgtggtta atgctgtcct caccaataaa ttccaggttg ccatcgctat cttgtttata
     1141 taccttgata atccctttgg gaagaggcat tccagcaccg ttatctacag tatttttgaa
     1201 tttgatgata cttttaacat tagtggcata ggtgggatat tgatattcag cataagcttt
     1261 aatgtttttg ggaggataaa gttccagttg ttttgtctga ttattggcaa aggagacctt
     1321 ttggtctaaa gtatacatat gaaagtcgtg aaacgccttt tcttcaaagg aaggagcggc
     1381 agattctggt gccatacctg catcataact gattaaactg cctctttttc cataatagcc
     1441 ggaataaacc cggttgatgt ctcccgcaat aagctttagc ataacatcgt taaatgcttt
     1501 gccggaagtg ttattgatgg ttacccagga attaaattgt aaggtttttt cgtcccaaac
     1561 cgtgttatag gtaacatccc agcttaagcc acccgttaaa taagacattt gaacaggata
     1621 ttttgctttt ttagaggcaa ttatgctcca atgcaaggtt ggttttgtgt aaaaattatc
     1681 tggcaattca gccagctgaa tccactgagt ttcgctttcg gaaattacca ataagcgttc
     1741 agttcctttt tcaataatac ctatgctgtt gtcactataa aacttgagaa taccggttaa
     1801 tctggattgg tctttcgtga taattagaac ctccctatcc agatatttag ccataatctg
     1861 ccattttcca gctaaatcgt attcaaaatt ctgctccgct atgcgaattc catttccagt
     1921 aacaattacg gatgaagggt caattctact ggttatatcg ctaaaattga tatcctggcg
     1981 tccattttcc agctccagct caaaacggct gcgaactaaa gaaagatcat tattgtaaat
     2041 tgttaaccag tcctctgcca ataaaggtaa ggcaaacagc agaaggataa tgactgtgag
     2101 aaag
//
LOCUS       DAOPFL010000191         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_75683_length_2087_cov_85.566929, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000191 DAOPFL010000000
VERSION     DAOPFL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75683_length_2087_cov_85.566929"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            469..1350
                     /locus_tag="PLG69_07850"
     CDS             469..1350
                     /locus_tag="PLG69_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPI43455.1"
                     /translation="MLLQKYFETRFELIDQELKNALTFEDKYATELKKAIYEAVIPGG
                     KRWRPLLLLSMFEMLTGFKKHRAFPDALKSSVAIELLHNGAIVHDDLPSVMNRTQRRG
                     QPALHIKYGNAIAILAADALYSLAYEIIGELKNPEQSLEAIRILSNATKSYGMIGGQV
                     VALSSRHKVMKINTLNYIDMKKVGSLLQASADIACLLSRADEETHQVMRAYALNLGIA
                     YQMIEDIEADYSRGSEGLDEEYVPVSKASYTALLGFDKARKTVENMLADSAKMLKPYP
                     NNSVLLEFVAMIKERLP"
     gene            1354..>2087
                     /locus_tag="PLG69_07855"
     CDS             1354..>2087
                     /locus_tag="PLG69_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HPI43456.1"
                     /translation="MVDIHTHLLPNIDDGSKSLNDSLSQLCQMAEGGIKRVYLTAHYF
                     KGHYQYSRQEYDEKFNTIVNKVKDAGIDLDIQPGFEIYLQPDILEDIKTHNLTMGKSS
                     YILLESDLNGLPNDFYNNIFPLLRAGYKPILAHAERYVSIMQKPSRAEDLIYRNIYMQ
                     TNAGSLLGLYGEKVKSTAWILVNKGWTHFIASDDHIRGKYLAYFEARDKVEEMIDNHT
                     AHLLFYVFPSCIASGENIPYEYVQVYR"
BASE COUNT          656 a          366 c          435 g          630 t
ORIGIN      
        1 aagcccggag ggcgacacaa tgatagcgat ggtggcgtaa gcccctcgta aaaagtaagc
       61 cccgattatt tttcttttat ttacctttcc cggttcccaa aagccccaaa gggcgacaca
      121 atgatgtata agggtatgta agcccctcgt ataaaggttt agaaggttta tagggttgag
      181 tgagtcatcc tgaaaaagtt aacacgcgaa ctaatgttcc aggagaaaga aaataaatca
      241 aaatcaaaac caaagagtca cttattatcg ctataatgtt gattataaaa ttcaggtttt
      301 tgtatgcgga ggaacggtgt cctccagcta caattttttc ggtgttctca gtgtcttcgg
      361 tgatttcggt ataaaataga gaaaaacctt gacaaaacta tggtatttta taaaaaagaa
      421 gaagttaaat agcagctttt gtcaagcttg atgaaaaggg agtatgcttt gcttttacag
      481 aagtattttg agacgcgttt tgaacttatt gaccaggaac ttaaaaacgc actaacattt
      541 gaagacaaat atgccacaga gcttaaaaaa gcaatttatg aagcagtaat tcccggaggt
      601 aaaagatggc gtcctttact gcttctttct atgtttgaaa tgcttacggg atttaagaaa
      661 catcgcgctt ttccggatgc tctaaagtcc tcagtcgcta ttgaattgtt acataatgga
      721 gccatagttc acgatgattt gccaagtgtg atgaatagaa cacagcgaag aggtcaaccg
      781 gcattgcata taaaatatgg taatgccata gctattttgg ctgctgatgc actttattct
      841 cttgcttatg aaattatcgg cgagcttaaa aacccggaac aatctctgga agcaattcgc
      901 attctatcca atgccacaaa aagttatgga atgattggag gacaggtagt agctctttcc
      961 agccggcata aagtgatgaa aataaacacc ttgaattata ttgatatgaa aaaggtcgga
     1021 tcgttattac aggcatctgc cgatattgcc tgtttgctaa gtagagcaga tgaagaaaca
     1081 catcaggtta tgagagctta tgccttgaat ttaggaattg cctatcaaat gattgaagat
     1141 attgaagcgg attattctcg cggaagtgaa ggtttggatg aagaatatgt tcctgtttcc
     1201 aaagccagtt atacagctct gctgggtttt gataaagctc gtaaaacagt agaaaatatg
     1261 cttgccgatt cagctaaaat gctgaagccc tatcctaata actctgtttt attggaattc
     1321 gtagctatga taaaagagag attaccctga tcaatggttg atattcatac tcacctgctg
     1381 cctaatattg acgatggttc aaaatcccta aatgattctc tcagtcagtt atgccagatg
     1441 gcagaaggtg gaattaaaag agtttattta acggcacatt actttaaggg tcattatcaa
     1501 tacagtcgtc aggaatatga tgagaaattc aatactatcg ttaataaagt aaaagatgca
     1561 ggtatagacc ttgatattca acccggtttt gagatttatt tgcaacctga tattttggaa
     1621 gacataaaaa cccacaatct gactatgggt aaaagttcgt atatcctttt ggaaagcgac
     1681 ttaaatggtc tgcccaatga tttttataat aatatctttc ccctattacg tgctggttac
     1741 aaacctattt tggctcatgc ggaacgctat gtgagcatta tgcaaaaacc ctctcgcgct
     1801 gaagacctga tttatcgtaa tatttatatg caaaccaatg ccggaagctt gcttggactt
     1861 tatggcgaga aggttaagtc cactgcctgg attttagtaa ataagggttg gacacatttt
     1921 attgcttcgg acgatcatat tcggggtaaa tatcttgcct attttgaagc gcgcgataag
     1981 gtagaagaga tgattgataa tcataccgcg catttgcttt tttatgtttt cccttcctgc
     2041 attgccagcg gagaaaatat cccttatgaa tatgttcagg tttaccg
//
LOCUS       DAOPFL010000192         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_76402_length_2073_cov_67.791378, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000192 DAOPFL010000000
VERSION     DAOPFL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76402_length_2073_cov_67.791378"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLG69_07860"
     CDS             complement(<1..1177)
                     /locus_tag="PLG69_07860"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPI43457.1"
                     /translation="MKGKILILEDDIVLSEQIANVVKHYNYEVMQTINSDQFFEVLRQ
                     FQPDVILLDVFLVGSKLNGIQVLQHLKNDMDLNYKVIVISGEVTSSQINEIRTLGAYH
                     FIEKGSAFSINQLLLHIENAITLKRQEEEHIGLQIEYINLKKQFARSFPFIGESNAIK
                     KVREQIIRLASVDEDIFLIGETGTGKEVAANYYYIYSPRFDKPFHTVNCSALSETLIE
                     SELFGHIKGSFTSADRDKTGFFEECSNGILFLDEVTNLSLMSQSKILRAIENKEIQVV
                     GGNLKKVDTRLIFASNAGLEKLSDESIFRKDLFYRIEGNIVELPPLRERDDDILLLIC
                     YFLTSFSSQYRVIDRLDLPALKDDLLNYSWPGNVRELRNFCKFIMINEQKINNNVIKK
                     "
     gene            complement(1184..>2073)
                     /locus_tag="PLG69_07865"
     CDS             complement(1184..>2073)
                     /locus_tag="PLG69_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HPI43458.1"
                     /translation="ETYYHSLAFLFDTNEFLDQVYLHRDLMQTEFGYYTTTFRNTELI
                     YQDRLSDIIFEIEGFKTILTEGVDRILQWRSPLYAYKNYSKNMNLLLKYYQLADDIAF
                     RFSNRDWPEYPLTVDKFVHWIDQLTLAEKGGKNLFLNLFMDYETFGEHQWASTGIFDF
                     MRHFPDAVLEREHLGFANPKDVSHLANYQQESLSFPEPVSWADEQRDISAWLGNEMQQ
                     NASETFYELLNRIKEKGDKELLRTARMLSTSDHFYYMCSKYFQDGDVHKYFSPYDSPD
                     QAYIYYINALAELEELLLR"
BASE COUNT          682 a          385 c          378 g          628 t
ORIGIN      
        1 gttttttaat aacattatta tttattttct gttcattaat cataatgaac ttgcaaaaat
       61 tacgcagttc tcttacattt ccaggccaag aatagttaag taaatcatct ttcagagcag
      121 gtaggtctaa acggtcaata acgcggtatt gagaagaaaa actggtgagg aaataacaaa
      181 taagaagcag aatgtcatca tctcgttccc gtaaaggtgg aagctcaaca atgttacctt
      241 caatgcgata aaacaggtct ttgcggaaaa tactttcatc ggagagtttt tccaagccag
      301 cattagaagc aaaaataagg cgggtatcaa cctttttcaa gtttccacct acaacctgta
      361 tttctttgtt ttcaatagcg cgtaggattt tagattgaga cattagggaa agattggtta
      421 cttcgtctaa aaagagaatg ccattggaac attcttcaaa aaagccggtt ttatcgcgat
      481 cagcactggt gaaagaacct tttatgtgac caaaaagttc tgattctata agggtttcgg
      541 ataaagcact gcaatttacg gtatgaaagg gtttgtcaaa acggggagaa tatatgtagt
      601 aataatttgc agctacttct ttgcctgttc ccgtttcgcc aatgaggaaa atatcttcat
      661 ccacagaggc aaggcggata atttgttccc taactttttt aatagcatta ctttcaccga
      721 taaagggaaa acttctggca aactgttttt tgaggttgat atattctatc tgcaagccaa
      781 tatgttcttc ttcctgtctt tttagagtga tggcattttc tatatgcaac agcaattgat
      841 tgatggaaaa ggcagaaccc ttttcaataa aatgataggc acccaaagtg cggatttcat
      901 ttatttgaga agaagtgacc tctcccgaaa taactattac tttataattc aaatccatat
      961 cgttttttaa gtgttgcaaa acctggatac cattaagttt ggagccaact aaaaaaacat
     1021 caagcagaat cacatccggt tgaaattggc gtaagacctc aaaaaactga tcgctattaa
     1081 tagtttgcat aacttcatag ttatagtgct tcaccacatt ggcaatttgt tcggaaagaa
     1141 ctatgtcatc ttccagaatc aaaatttttc ctttcatatt gcattacctt aaaagaagct
     1201 cttccagttc cgccagagca ttaatataat agatatatgc ttgatccggt gaatcgtaag
     1261 gagagaagta tttatgcaca tctccatcct gaaaatattt ggaacacata taataaaaat
     1321 ggtcggaagt gctaagcata cgagcagttc taagcaactc tttatctccc ttttccttaa
     1381 tcctattcag caattcataa aaggtctcac tggcattttg ttgcatttcg tttcccagcc
     1441 aggcagaaat atccctttgt tcatcagccc aggaaaccgg ttccggaaaa gaaagcgatt
     1501 cctgttgata attagccaga tgagaaacat ctttaggatt ggcaaaaccc aaatgttctc
     1561 tttctaaaac agcatcgggg aaatgacgca taaaatcaaa aatacctgtg gaagcccatt
     1621 gatgttctcc gaaggtttca tagtccataa aaaggttcag aaacaggttt tttccgcctt
     1681 tttccgctaa ggtgagctgg tctatccaat gcacgaattt atctacggtt aaaggatatt
     1741 caggccaatc ccgattggaa aaacggaaag caatatcatc tgccaattga taatacttaa
     1801 gcaacaaatt catatttttg gaataattct tataggcata caaaggactg cgccactgga
     1861 gaattctatc tacaccttcg gtaagaatgg tcttgaatcc ttcaatttca aaaataatat
     1921 cagataagcg gtcttgatag attaattccg tattgcggaa agtggttgta taatagccaa
     1981 attcagtttg catcaagtcc cgatggagat aaacctgatc aagaaattca ttggtatcaa
     2041 aaagaaaagc caaagaatga taataggtct cac
//
LOCUS       DAOPFL010000193         2060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_77080_length_2060_cov_91.764090, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000193 DAOPFL010000000
VERSION     DAOPFL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77080_length_2060_cov_91.764090"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /locus_tag="PLG69_07870"
     CDS             <1..1724
                     /locus_tag="PLG69_07870"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPI43459.1"
                     /translation="TVFMALGAWRMSKKNVLVRRLQAIESLGSATVLCTDKTGTITYN
                     KMTLQKIVSNSENIELKNSAKLSEPILNTLIHSVLGSMREPFDPMEKAIHNALNDLDP
                     FTYTELKNYQEIREYPLTDELRAMAKVWKISGKTSYTVALKGSPEAVIKLCSVNTEKI
                     NTIMNQVSDLAEEGLRVLGIASCEIKTDVLPEKLTDLSFRYLGLIGLADPIRETVPEA
                     VKICYKAGIRVIMITGDYIGTAKAIGKQIGLNNVDNIISGSELDQMTDEELAEKCKQV
                     SIFARVVPQQKLRIVQALKANGEIVAMTGDGVNDAPALKAANIGISMGLRGTDVAREA
                     SAIVLTDDDFSSIVSAVRMGRRIYDNLRKSFTYVFSLHIPIAGMAMIPASFADYPIIY
                     FPVHIAFLELIIDPACSIVFEMEKEEDDIMERPPRSVDEKLFGTKRLIIGFLQGLGVL
                     ILLVLMYFWGNNRGMGENHLRAHIFTTLVFSNLMLITTNRSWHKNLFQIIRTPNKGMT
                     YLFLGVFAIVLLMIKVPSVTNIFHLETMSIMELLTCLILGNMSVAWFEIYKLIVNRKS
                     ANKHLTE"
BASE COUNT          692 a          331 c          421 g          616 t
ORIGIN      
        1 taactgtttt tatggcgtta ggtgcttgga ggatgtccaa aaaaaatgtg ctggtcagaa
       61 ggttgcaggc aattgaaagt ttgggttctg ctacagtttt atgcacagat aaaacaggca
      121 caataactta caacaaaatg accttgcaaa aaatcgtcag caacagcgaa aatatagagt
      181 taaagaattc ggcaaagctt tcagagccaa ttcttaatac tcttattcat agtgtccttg
      241 gcagtatgag agaacctttt gacccgatgg aaaaagcaat tcataatgca ctgaatgatc
      301 ttgatccctt tacttataca gaactgaaaa attatcagga aattagagaa taccctttaa
      361 ccgatgaact tagagcgatg gcaaaggttt ggaaaatttc cggcaaaaca agttacacag
      421 ttgcgttaaa aggttcaccc gaggcagtta ttaaactctg cagtgttaat acagaaaaaa
      481 tcaataccat aatgaatcag gtttctgatt tagcagaaga agggcttaga gttttaggaa
      541 ttgcctcttg tgaaattaaa acagatgttt taccagaaaa acttactgac ttatctttcc
      601 gctatttggg tttaatcgga ctggcagacc ctattagaga aactgttccg gaagcagtta
      661 aaatctgtta taaagcagga atcagggtaa taatgattac cggtgattat attggaacgg
      721 caaaagcgat aggtaaacaa ataggtctga ataatgttga taatattata tcgggtagtg
      781 aattagacca aatgaccgat gaagaacttg cagaaaaatg taaacaggtc tctatttttg
      841 cccgcgttgt tccacagcag aaattaagaa ttgtgcaagc acttaaggca aatggagaaa
      901 tcgtggctat gacaggagac ggagtgaatg atgctccagc tttaaaagct gccaacatag
      961 gtatttctat gggtttaaga ggaactgatg tagcccgtga agcatcagca attgttttaa
     1021 ccgatgatga tttttcttcc attgtaagcg ctgtcaggat gggtagaagg atatatgata
     1081 acctccgtaa atcattcact tatgtttttt ctctgcatat tcccattgcc gggatggcaa
     1141 tgattcctgc ttcatttgcc gattatccga tcatctattt tccggttcat atcgcttttc
     1201 tggaattaat tatagaccct gcttgttcta ttgtttttga aatggaaaaa gaagaagatg
     1261 atataatgga aagacctccg cgctctgtgg atgaaaaatt atttggcact aaaagattaa
     1321 ttatcggttt tctacaaggt ttaggtgtat taatcctgtt agtgctgatg tatttttggg
     1381 gaaataaccg cggaatggga gaaaatcatt taagagccca tatttttact accttagtgt
     1441 tttccaatct aatgctgata actacaaaca gaagctggca taaaaattta ttccagatta
     1501 tccgcactcc caataagggt atgacttatt tgtttttagg tgtctttgcc attgtcctgc
     1561 tgatgataaa agtgccttct gttactaata tttttcacct ggaaacaatg agtataatgg
     1621 aattattaac ctgcctgatt ttaggaaaca tgagtgttgc ctggtttgaa atatacaaac
     1681 ttattgttaa taggaaatca gcgaataaac acttaacaga gtaacagaat ggcaacagat
     1741 tgtatttttt acactcagaa ctccgagaac accggaataa attttcaaat aaatttatga
     1801 aagtcaggag tcgtcactga ctatatagtt tatacgctat acactaattc tgttggcaga
     1861 gaggactaat gacgactcgt agcaagaaga ttgtttcaca aagaaagaaa gagaaatgac
     1921 ttagaacact aagagaaaaa gcgagaaaga gaaataattg agaatgaaaa taaagtcgtt
     1981 gtaattatca ataaacctcg gtgtactcag tgtttttggt gatctcggtg taaaaataaa
     2041 tcagcgcaat ctgcgtgctt
//
LOCUS       DAOPFL010000194         2015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_79646_length_2015_cov_123.955612, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000194 DAOPFL010000000
VERSION     DAOPFL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79646_length_2015_cov_123.955612"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLG69_07875"
     CDS             complement(<1..272)
                     /locus_tag="PLG69_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43460.1"
                     /translation="MKRLFLAILALTLSIGGILSAQTLVNIGSGTAVNTTTGVPTPYG
                     TFYKNFRQQYLILASELNDAGGGAGDITSIAFNVSNLNNCSPMPNYT"
     gene            complement(549..1202)
                     /locus_tag="PLG69_07880"
     CDS             complement(549..1202)
                     /locus_tag="PLG69_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPI43461.1"
                     /translation="MLKKMFLKTTKKAEHEKNSLIIIDNEVRGILPDRILLSYFPLPF
                     AGEIDDEQGKELLSLIAKNARQQLLKYVSDQEHSSVQCRQFLSRKHYPAELIDNLIKE
                     FQNKKYIDDFRFVQILISSLIERKKSKRAIAQKLKETRLPNELWERKLNELYPLEEEK
                     ENLKEQIVKLRFRYRDLPLNKQKEKVFASLYRKGFDLDAIHSAWQECSSNRFLPSQE"
     gene            complement(1162..>2015)
                     /gene="recA"
                     /locus_tag="PLG69_07885"
     CDS             complement(1162..>2015)
                     /gene="recA"
                     /locus_tag="PLG69_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPI43462.1"
                     /translation="ASGKTTLALHIAAECQKQGGIVAFIDVEHALDPIYAKRLGVITD
                     DLLLSQPDGGEQALEITETLVRSSAVDLIIIDSVAALVPKQEIEGEMGDSHVGLQARL
                     MSQALRKLTAIVSKSNTAVIFINQTRMKIGAGPYMNPETTTGGVALKFYASVRLEVRH
                     GAQIKDVGSESQVIGTRTKVKVVKNKFAPPFKTVEFPIIFGQGISKLDIILEMAVQHD
                     IIKKSGSWFSYNDLKLGQGADKTKLYLTENQPLLQEIEAKLREKINPEEFNLSAEENV
                     PEDNEES"
BASE COUNT          554 a          402 c          392 g          667 t
ORIGIN      
        1 gtgtagttcg gcatcggact acaattattt aaattactga cattgaaggc aatagaagtg
       61 atatcaccgg cacctccacc tgcatcattc agttcggatg ctaaaattaa atattgctgc
      121 cggaagttct tataaaaagt tccataggga gtgggaactc ctgttgtagt attaactgca
      181 gttccacttc caatattaac caaagtttga gcggaaagaa ttcctcctat gctcagcgtt
      241 agggcaagaa ttgctaaaaa taagcgtttc ataaccttct ccttgattta gctatacttg
      301 tttacgggtt ttacacatcc ggagtacaca taaatattac atccggctga aacacataca
      361 cactatgaca ttaaaatagg ttaacatctc tgtgaactaa taaattatag cgcacaataa
      421 taaatccatc tttacctaca tttgaagcaa tttttatacc cattacatag aaaatattgc
      481 gaggcatttt ttctctcttc cactttaatt agcaaaagca ggcctacctg tcaaacctgc
      541 gttttttgtt attcctgcga aggtaggaat ctattactac tacattcttg ccaagcagag
      601 tgaatagcat ccaagtcaaa acctttacga tagagggagg caaagacctt ttctttttgt
      661 ttgttgagag gcaaatcgcg atagcggaaa cgcagtttta caatttgttc tttcaggttt
      721 tctttttctt cttcaagagg gtatagttcg tttaattttc tttcccacag ttcatttggc
      781 agccgtgttt ctttcaattt ctgtgctatt gcgcgtttgc ttttttttcg ttcaatgaga
      841 gagctaataa ggatttgcac gaagcgaaag tcatcaatat attttttgtt ctgaaattct
      901 tttatgagat tatcaatcaa ttctgcagga tagtgttttc gggaaaggaa ttgtcggcat
      961 tgaacagagc tgtgttcttg atcggataca tatttcagga gttgttgccg ggcatttttg
     1021 gcaattagtg aaagcagttc tttgccttgt tcgtcgtcaa tttccccggc aaaaggaaga
     1081 gggaaataag aaaggaggat tctatcagga agaatccccc ttacttcatt gtcaataata
     1141 atcagcgagt ttttctcgtg ttcagctttc ttcgttgtct tcaggaacat tttcttcagc
     1201 acttaaattg aattcctcgg gatttatttt ttcccttagt ttggcttcaa tttcttgtag
     1261 cagtggctgg ttttccgtta aatagagctt ggttttatct gctccctgac ccagtttgag
     1321 gtcattgtaa gagaaccagg aaccgctttt cttgattata tcatgctgaa ctgccatttc
     1381 cagaatgata tccagtttgg agataccctg gccaaaaata ataggaaatt ctactgtttt
     1441 aaagggagga gcgaatttat ttttcactac ttttactttg gtacgagttc cgataacttg
     1501 tgattcggag ccgacatctt taatctgggc accatgccga acttctaagc gcactgaagc
     1561 atagaatttt agagctactc caccggttgt ggtttcaggg ttcatatagg gaccggcacc
     1621 tattttcatt cttgtctgat tgataaaaat aactgcagta ttgcttttgg agacgatagc
     1681 agttaatttt cgcagtgcct ggctcatcag tcgtgcctgt aaaccaacat ggctatctcc
     1741 catttcacct tcaatttcct gtttgggaac cagtgcagcg acggaatcaa tgattattaa
     1801 atcaactgca gaactgcgaa caagagtttc agtaatttcc agtgcctgtt cacctccatc
     1861 gggttgagaa agcaaaaggt catctgtaat tacccccaag cgcttggcat aaataggatc
     1921 aagggcatgt tcaacatcaa taaaggcaac tattccaccc tgtttttgac attctgccgc
     1981 aatatgcaaa gccagagttg ttttacctga tgctt
//
LOCUS       DAOPFL010000195         2008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_80032_length_2008_cov_69.291859, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000195 DAOPFL010000000
VERSION     DAOPFL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80032_length_2008_cov_69.291859"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1071)
                     /locus_tag="PLG69_07890"
     CDS             complement(292..1071)
                     /locus_tag="PLG69_07890"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI family restriction endonuclease"
                     /protein_id="HPI43463.1"
                     /translation="MSDRIIKDFKKYRDFLATIPLDKYREELKDIKWVEQDLPKEILP
                     LASIFKYYWEERQFLDFEEWFDTFWKEINTNSPSKEVLNQFKKYYFDKDNNGWFKKGF
                     RARMYRTWVSVLTQLDFCYLFQFLCAREGKILSLECNAALDARGVDAKVGNIGFQVAK
                     ISNRKEARSASKKKTLVTIPYAVFNIEEFEKKIKSPRVKDKTGYQNSLKAFQKYFIRL
                     KNGFVVFGEAYLLPIIDNIEDITKVKETVDKISLELSGEEV"
     gene            complement(1064..>2008)
                     /locus_tag="PLG69_07895"
     CDS             complement(1064..>2008)
                     /locus_tag="PLG69_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HPI43464.1"
                     /translation="KTNPATKEKYKKLYATWFGKYNVYGAFIEKSINLLKDNGQLIFI
                     VPPTFMILDEFKKLRVFLAQNGKTEIIYLGADIFKPEADVSSVVLNFIKSDKYISRLE
                     LKEFQDNKIRTLKSNFHWKGEVVKFDTDYTQTLESLCPYRLGDIYNIRISPRTPEIKR
                     SNIIVKEKAPGENDYLPLLNGKNLKCNMINYNNLTGYWVKKQDVNKIRMYFGYPHIVV
                     GLGFRENGKVAAAFDEKCYPWMGDVYHLVRENSLFNCHFDLETEEVVAYLNSDYIKKY
                     VKDTYREITYHLSITQLKNLPVPDKNTLQLIKKENLCE"
BASE COUNT          624 a          363 c          278 g          743 t
ORIGIN      
        1 gaccaacttg gcaataagat tttagaacct ctcacaaatt acacagatag attttgaagc
       61 aaggatttta ggatataagg atttgtgtcg ttctaaataa ttttgcacta aatcaaggaa
      121 aggagatatt gaaaataagc tttacgcctg aagttaatta gttatgatat ttaggggctg
      181 taaactataa cttttctgtc taatcacgct gcatataaat gatctcacaa ggtctgagat
      241 tgttaacttt atcatttgct aaaaactatt atttgccatt atgaatcata tttacacttc
      301 ttcacccgat agttccaaag atattttatc taccgtctct tttacttttg tgatgtcctc
      361 tatgttatct atgataggta gaaggtatgc ttctccgaaa acaacaaaac catttttgag
      421 acggataaaa tatttttgaa atgctttcag tgaattttgg taacccgttt tatcttttac
      481 ccttgggctt ttaatcttct tttcaaattc ttcaatatta aaaactgcat aaggtattgt
      541 taccaatgtt ttctttttgc tggcactacg tgcttctttt ctattactaa ttttagcaac
      601 ttgaaatccg atatttccaa ccttagcgtc aacccctctg gcatctaaag cagcattaca
      661 ttccagcgat aaaatcttac cttctcttgc acaaagaaac tgaaataaat aacaaaaatc
      721 caattgagtg agcacagaga cccaagttct atacattctg gctctaaaac cttttttgaa
      781 ccagccgtta ttgtccttat caaaataata tttcttaaat tggttaagta cttctttact
      841 tggagaatta gtattaatct ctttccaaaa ggtatcaaac cattcttcaa aatccagaaa
      901 ttgtctttct tcccagtaat atttaaatat tgatgccaga ggtagtatct ctttaggcaa
      961 atcctgttca acccatttaa tatcttttaa ttcttcgcga tatttatcca ggggaatagt
     1021 tgcaagaaaa tcccgatatt tcttgaaatc tttaattatt ctgtcactca caaaggttct
     1081 cctttttaat aagttgtaaa gtatttttat caggtacggg caaatttttt agctgagtaa
     1141 tactaagatg ataagtaatt tcccggtaag tgtcttttac atactttttt atataatcag
     1201 aatttaagta tgcaactact tcttctgttt ccaaatcaaa atggcagtta aataaactgt
     1261 tctctcttac aagatgataa acatcaccca tccagggata acatttttca tcaaaagcag
     1321 cggcaacttt gccattttct ctaaaaccca agcccactac gatatgaggg tagccaaaat
     1381 acattctgat tttgtttaca tcttgctttt taacccagta accagtaaga ttgttataat
     1441 ttatcatatt acattttaga ttttttccat ttaataaagg aagataatca ttttcaccag
     1501 gtgctttttc tttcactata atgttacttc tttttatttc aggagttctg ggtgaaattc
     1561 taatattgta aatatctcct aacctatatg gacataagct ttccaatgtc tgtgtataat
     1621 cggtgtcaaa cttaacaact tcacctttcc agtgaaaatt actttttagc gtcctgattt
     1681 tattatcttg aaattctttc agttccagcc gggagatgta tttatcggac ttaataaaat
     1741 tcaacactac cgatgaaaca tctgcttccg gtttaaatat atccgctcct aaatagatta
     1801 tttccgtctt cccgttttga gctaaaaata ctcggagttt tttgaattca tccagaatca
     1861 taaaggttgg aggaacaata aatatcaatt gtccgttatc tttaagtaag ttaattgatt
     1921 tttcaataaa agcgccataa acattatatt tcccaaacca tgttgcataa agttttttat
     1981 atttctcctt ggttgcagga tttgtttt
//
LOCUS       DAOPFL010000196         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_80241_length_2004_cov_78.158543, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000196 DAOPFL010000000
VERSION     DAOPFL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80241_length_2004_cov_78.158543"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..899)
                     /locus_tag="PLG69_07900"
     CDS             complement(168..899)
                     /locus_tag="PLG69_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPI43465.1"
                     /translation="MEEQLKTLAKMQTLDDEIGRYRVLQKELPKQLNEIIESVEQATA
                     NLLTVETERAEINKKQRTLEMDIKQLQEQQKKYTNQLAEIKNNKEYKALNSEIAYIKD
                     KISNLESNLLELMDQENEIKEKVASAKAELEKAETRKKEKETDLREQIASLETKIEQV
                     RTKRNDLARTLPVPVVKHYGNMIKHKNNQAVAYNRNGSCGACGFVIRQQMRIELQLRR
                     KIIYCENCGRILMNRFEDNSEDASN"
     gene            complement(903..1724)
                     /gene="nadC"
                     /locus_tag="PLG69_07905"
     CDS             complement(903..1724)
                     /gene="nadC"
                     /locus_tag="PLG69_07905"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPI43466.1"
                     /translation="MFIDEIIRKGLEEDIGSGDITTRYLDLEPQNNIAYIIPKEEGVL
                     AGVDVARLVFRMVDPELKIVLYNKDGDKIIPNEEIMRLEGRPSSILQGERTALNFLQR
                     LSGIATKTAKFVSLLEGTNVKLLDTRKTTPLLRSLEKYAVRVGGGYNHRFGLFDMVLI
                     KENHIRACGSITEAVNRISKHNTSYKIEVEVTNLKEVDEAAKAKVDRVMLDNMTPAQI
                     RKAVKKYKDILEFEVSGGITEENIRSFAETGVQFISSGALTHSYKSLDISLLFKE"
BASE COUNT          515 a          406 c          356 g          727 t
ORIGIN      
        1 tctgtggagc ttgctctgca ttactgcagc ttcccgttag gaagtatcct gccctatggt
       61 gtccggactt tcctctgcaa tttatttgca gtgaacatca aatcttccct ctttaaataa
      121 taattcaaaa ctgcgaaaaa acaaatttcc cataaccagg ggcaaaatta gttggaagca
      181 tcttccgaat tatcttcaaa gcggttcatc aaaatgcgac cgcaattttc acaatagatg
      241 attttgcggc gcaactgaag ttctattctc atttgttgac gaatcacaaa tccgcaggca
      301 ccacaggaac cattacgatt gtaagcaact gcttgattat ttttatgttt aatcatattg
      361 ccatagtgtt taaccaccgg aacgggtaag gtgcgtgcca ggtcatttct tttagtgcga
      421 acttgttcaa ttttggtttc cagggaagca atctgttctc ttaaatcggt ttctttttct
      481 ttttttcggg tttcagcttt ttccagttca gctttagcag aggcgacttt ttccttaatt
      541 tcgttttcct ggtccatcag ttctaaaagg ttggattcca gattggagat tttatctttg
      601 atataggcaa tttcgctgtt taatgcttta tattctttgt tgtttttgat ttctgccaat
      661 tggttggtat attttttttg ttgttcctgc aattgtttta tatccatttc cagggtacgc
      721 tgttttttat taatttctgc tctttcagtt tccacggtaa gaaggttggc agttgcttgt
      781 tctacgcttt caataatctc gtttagttgt ttgggcaatt ctttttgtaa aaccctgtag
      841 cgtccaattt catcatccag ggtttgcatt ttggctaaag ttttcagttg ttcttccata
      901 gtttactcct taaataacaa actgatatcc agtgacttat aagaatgagt gagggctccg
      961 gaagaaataa attgcactcc tgtttcggca aaagaacgaa tgttttcttc tgtaatgcct
     1021 cctgaaacct caaattccag aatatcttta tattttttta ctgcctttct tatttgcgca
     1081 ggagtcatat tatccagcat aactctatcc actttagctt tagctgcttc gtcaacttct
     1141 tttagatttg taacttccac ttcaatttta tagctggtat tatgtttact gatgcgattt
     1201 actgcttccg tgatagagcc acaggcacga atatgatttt ctttaatcaa caccatatca
     1261 aataagccaa aacggtgatt atagccacct cctacgcgga cagcatattt ttccagggaa
     1321 cgcaaaagag gagttgtttt acgggtatcc aaaagtttta catttgttcc ttccagcaag
     1381 gaaacgaatt ttgccgtttt agtggcaatt ccggaaagcc gttgcagaaa gtttaaggca
     1441 gtcctttcac cttgtaaaat ggaagaagga cgcccttcca ggcgcataat ttcttcattg
     1501 ggtataattt tatcgccatc tttgttatag agaacgattt tcagttccgg atcaaccatc
     1561 cgaaaaacga ggcgtgcaac atctactcct gccaagacac cttcttcttt cggaataatg
     1621 taggcaatat tattttgggg ttccaaatcc agatagcggg tagttatatc tccacttccg
     1681 atatcttctt ccagaccttt acggattatt tcatcaataa acacgctacc tccaaggcaa
     1741 aaaaaaagca tctgcgtttt gcaaatgctt tttttctatt gtatgttcaa tcttgtcagg
     1801 ttggcataat ttcttgtttt ggtaacacat ataactatct tcctcttatt tatttacttt
     1861 tattttgtct ccctgcaatc tgtcaagaca aaatattctt ttctcttact tttttggctt
     1921 gacgaaaatc tgcctcaggg attttagggt tttaaatgta aatcagggca gggaattccg
     1981 acttgccaaa gctgataaaa tcag
//
LOCUS       DAOPFL010000197         1989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_81096_length_1989_cov_108.937952, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000197 DAOPFL010000000
VERSION     DAOPFL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81096_length_1989_cov_108.937952"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1372)
                     /locus_tag="PLG69_07910"
     CDS             complement(59..1372)
                     /locus_tag="PLG69_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPI43467.1"
                     /translation="MANKDQSTLFDREELSTKTVPLAEKLRPQTLEELLGQTKLIAEN
                     SPLRQMIESGEYHSFILWGPPGSGKTTIARIIEKNSGYNFIRFSAVLASISDVKAVMK
                     EADYLHRTQNKRSILFIDEIHRFNKSQQDAFLPYVESGAIILIGATTENPSFEVIPAL
                     LSRCTVFVLEPLSENDLKIILKRGFAELKLEEDEEVINWMAQNAGGDARRVLNDLELV
                     LPYLQKKPHPKTEELAKSLAKKTMFYDKNREEHYNLISALHKSLRGSDPQAGLYWLAR
                     MLEAGEDPRYIVRRLIRFASEDVGLADPNALVQAIAVKEAVLFIGMPEASTALAQLVV
                     YLATAPKSNALYTAYEEAAEDARKTSHYGVPLHIRNAPTQLMKDLNYGKDYHYDHEYE
                     YKYYYQKYFPEQMPEKIYYKPGTYGFEKEIQKRIDWWAKLKKEQK"
     gene            complement(1384..1851)
                     /locus_tag="PLG69_07915"
     CDS             complement(1384..1851)
                     /locus_tag="PLG69_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPI43468.1"
                     /translation="MTLEDSYRMVIAEEIRAQKMYQALAKSFAKPETSNVFKELVVLE
                     KMHEEKVRSAFAKEFPGQTITLQEEPIKEMQGVNWNDPQMVLEFAITKEEESVELYKN
                     MAEQTREPEIKKQLLQFAKEEEGHKSILLSEIQRLQGALQWFDPSELSGLMED"
BASE COUNT          544 a          450 c          354 g          641 t
ORIGIN      
        1 gcaaagaatt ataaagttga cttcgagtca gtatctaata ttgcattggc tgctcccttt
       61 atttttgttc cttttttagt ttagcccacc agtcaatccg tttctgaatt tctttttcaa
      121 aaccgtaagt tcccggttta taatatatct tttccggcat ctgttcgggg aaatacttct
      181 ggtaataata cttatattca tattcgtggt cgtaatgata atccttacca taattcaggt
      241 ctttcatcaa ttgcgtgggc gcatttctaa tatgcaaagg aacaccgtag tgacttgttt
      301 ttctggcatc ttccgctgct tcttcataag ctgtatagag ggcattactt ttaggtgcag
      361 tagccaaata aaccaccaat tgagctaaag cagtgcttgc ttcaggcatt cctataaaaa
      421 gaaccgcttc ctttactgca attgcctgaa ccaaagcatt cggatctgcc aaacccacat
      481 cttcgctggc aaaacgaatc aaacgacgca caatataacg agggtcttcc cctgcttcca
      541 acattcttgc cagccagtaa agtcccgctt gcgggtctga acctcttagc gatttatgta
      601 atgcggaaat caggttataa tgttcctcgc ggtttttatc gtagaacatt gtcttttttg
      661 ccagggattt tgccagttcc tcagttttcg gatgcggttt cttttgcaga taaggcaaaa
      721 ccagttccaa atcgttcaaa acccttctgg catcacctcc ggcattttgt gccatccagt
      781 ttataacttc ttcatcctct tccaatttca attctgcaaa accgcgcttc aatattattt
      841 tcaggtcatt ttcgctcaag ggctccaaca caaaaacagt gcaccgggaa agcaatgccg
      901 gaatcacttc aaaagatgga ttttccgttg ttgctccgat taaaattatg gctccacttt
      961 ccacataggg caaaaaggca tcctgctgac ttttattaaa acggtgaatt tcatcaatga
     1021 acaaaatact gcgcttgttt tgcgtccgat gcagataatc cgcttctttc atcactgctt
     1081 ttacatcact gatgctggca agaacagctg aaaaacggat aaaattgtaa ccgctgtttt
     1141 tctctataat cctggcgatg gttgtttttc cactacccgg aggaccccat aaaataaagg
     1201 aatgatactc tccactttca atcatctgcc ttaaaggcga attttcagct atcagcttcg
     1261 tttgacccaa tagctcttcc agcgtttgag gacgtaattt ttcagccaga ggaactgtct
     1321 tagtgcttaa ttcttctcta tcaaaaagag tgctttgatc tttatttgcc attatctaaa
     1381 agctcaatcc tccatcaatc cggataattc ggaaggatca aaccactgta aagcaccctg
     1441 caaacgctga atttccgaaa gtaaaattga cttgtgacct tcttcttctt tggcaaattg
     1501 cagtagttgt ttcttaattt ccggttccct ggtctgctct gccatatttt tatagagctc
     1561 aacggattct tcttctttgg tgatggcaaa ttccaagacc atctgcggat cattccaatt
     1621 tacaccttgc atttctttaa tcggctcttc ctgtagagtt attgtttgcc cgggaaactc
     1681 tttagcaaaa gcagacctta ccttttcttc gtgcattttt tctaaaacga ccagttcctt
     1741 gaatacattg ctggtttcgg gcttggcaaa actctttgct aatgcctgat acatcttttg
     1801 tgctctgatc tcttccgcta tcaccatccg gtaactatct tctaatgtca tttgtttcac
     1861 ctccctgtta ataacataat aatcagattg ggcaaaaaaa gcaaacggga tataatgcga
     1921 ggtggagaga tggaaaataa agaggaaaag agaaaatatt aacaaagaga taaagaggaa
     1981 aagagaaaa
//
LOCUS       DAOPFL010000198         1972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_82074_length_1972_cov_115.035994, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000198 DAOPFL010000000
VERSION     DAOPFL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82074_length_1972_cov_115.035994"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1026
                     /locus_tag="PLG69_07920"
     CDS             214..1026
                     /locus_tag="PLG69_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43469.1"
                     /translation="MLTIDWKERLNMDTADFLKNKLPKGDYDFEIIFIAYPERVNGKI
                     PAEVITHVASNIVQQLGKKHDAYLPFYRALWNKKGDYGKLAFGQILSKLLNRKPSVYL
                     PLLEEALSNATMAEINALLDKVMLPLLRKYPEKYLNKVFQYSNSSNPALQKSALNLLI
                     KLVKRREDLIPQIMTFFSRQWLSPLGEAVPYHITMLKTVAKLNPEYYLKIWEEFSFSH
                     DPQIVELLCTSITDYYPELESPVEIWTKSGNARLKKAATTAQRTLKKKKGAQ"
     gene            1030..1509
                     /gene="greA"
                     /locus_tag="PLG69_07925"
     CDS             1030..1509
                     /gene="greA"
                     /locus_tag="PLG69_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPI43470.1"
                     /translation="MDLSQFITKTGMQRLQKRINELLAERPEVIKAVAIAREFGDLSE
                     NAEYKAARERQRAIDNEIDYLRRRSANLKVIDTSEFPKDMVRFGTYCVAEDINTKECI
                     CYHLVGPEELNYYNEEGIQEVSIVSPIGKALLSKKIGEIAIVKAPIGERKLKIIEIK"
     gene            1525..>1972
                     /locus_tag="PLG69_07930"
     CDS             1525..>1972
                     /locus_tag="PLG69_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPI43471.1"
                     /translation="MKKTKQEKLTYEPRNFWKEATPEEQKKAMAYAIPYIEFLNKAKT
                     ERETIAYTLEILKNNKFSPIGKKGSRKVYSVFRNKTMAMAVIGSEPISKGFNMIAAHI
                     DSPRVDLKQNPLYEDINSSLSCMRTHYYGGIKKYQWVSTPLALHGVI"
BASE COUNT          687 a          343 c          399 g          543 t
ORIGIN      
        1 gcggactctg gatccgtatg tgagggttca actccttcca tcccagccag ttttataaaa
       61 aagggaagat ttgttttgga gggtttgcct tttaatgata ggaacgagtt aacttcttta
      121 atattatgcc agttatattt ttctattgac aggtaaaagg attttcaggc ataaggaaaa
      181 aagaaagaga aaaagtaaaa gcgggagaga taaatgctta ccattgactg gaaggaacgg
      241 ctgaatatgg atacagctga tttcctgaaa aataaactgc cgaaaggtga ttatgatttt
      301 gaaatcatct ttattgcata tccggaaaga gtaaatggaa agattcccgc tgaagttatc
      361 actcatgtag caagcaatat tgttcagcaa ctggggaaaa agcatgatgc ttatcttcca
      421 ttttacagag cgttatggaa taaaaaggga gattacggta agcttgcctt tggtcaaata
      481 ttaagcaaac tgcttaatcg taagccctct gtttatttgc ctcttttgga agaagcactt
      541 tctaatgcta cgatggcaga aatcaatgct ttattggata aagttatgtt gccgctttta
      601 cgcaagtatc ctgaaaaata tctgaataag gtttttcaat acagcaatag ttcaaatcct
      661 gctcttcaaa aatccgcctt gaatctattg ataaaactgg tgaaaagacg cgaagacctc
      721 attcctcaaa ttatgacctt tttttccagg cagtggttat cccctttggg agaagcggtt
      781 ccctatcata ttacaatgct aaaaacggtt gccaagctaa atcctgaata ttacttaaaa
      841 atttgggagg aattcagttt cagtcatgat cctcaaatcg tagaattgct ttgcacttca
      901 ataacggatt attatccgga actggagagc cctgtagaga tttggacaaa aagcggaaat
      961 gcccgtttga aaaaagccgc tactactgct caacgcactt tgaaaaagaa aaaaggagct
     1021 caataaaagt tggatttaag ccaatttatt accaaaaccg gaatgcaacg gttgcaaaaa
     1081 agaataaatg aactgcttgc cgaacgcccg gaagttatta aggcagtagc tattgcgcgc
     1141 gaatttgggg atttaagtga aaatgcagaa tacaaagcag ccagagaaag acaacgagct
     1201 attgataatg aaattgacta tcttagaaga cgcagtgcca atttgaaagt gatagatacc
     1261 agtgaatttc ccaaagatat ggtgcgtttc ggaacttatt gtgtagcaga agatattaac
     1321 accaaagaat gtatctgcta ccatctggtc ggtcccgaag aactgaatta ttacaatgaa
     1381 gaaggcattc aggaggtctc tattgtttct cccatcggca aagctttatt aagcaaaaaa
     1441 ataggtgaaa tagccattgt taaagctcca atcggagaaa gaaaactgaa gataatagaa
     1501 attaaataga taactggaga tataatgaaa aagacaaaac aagaaaaact tacctacgaa
     1561 ccccgaaatt tctggaaaga agctactcct gaggaacaaa aaaaggcaat ggcttatgca
     1621 attccctata ttgaatttct gaataaagcc aaaaccgaac gcgaaacaat tgcctatacc
     1681 ttagaaatat tgaagaacaa taagttttca cctattggta aaaaaggcag ccgcaaggtc
     1741 tatagcgttt ttcgcaataa gacaatggca atggctgtta ttggcagtga accaatttcc
     1801 aagggattta atatgattgc tgcccatatt gattctcccc gggtagacct aaaacaaaat
     1861 cccctttatg aagatattaa tagctcttta agctgtatgc ggactcacta ttatggagga
     1921 attaaaaaat atcagtgggt ttctactcct ctggctttgc acggagttat ta
//
LOCUS       DAOPFL010000199         1964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_82567_length_1964_cov_92.657936, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000199 DAOPFL010000000
VERSION     DAOPFL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82567_length_1964_cov_92.657936"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLG69_07935"
     CDS             complement(<1..653)
                     /locus_tag="PLG69_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HPI43472.1"
                     /translation="MDQDIWQNILDKLEENINPQSFKTWFSDTRLVDMNDRELMIRVA
                     TQFSANYLNQNYKEVLGEISYGLYGRKYDVQFITQPHRNHNKENDVQDYSIKRVSANV
                     RLNERYTFDEFVVGKNNNFAYSAAKAVAESPGYTYNPLFIYGESGMGKTHLMQAIGNY
                     ILKEGRNCSIYYITSEAFTNEMIDGIRSNKMPEFRAKFRKVDLLLMDDIHFLSRKEGT
                     "
     gene            complement(1746..1898)
                     /locus_tag="PLG69_07940"
     CDS             complement(1746..1898)
                     /locus_tag="PLG69_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43473.1"
                     /translation="MTLVDELKVMHLNQRILELWKRINTDSSAYFDDDKVKNTFDTIS
                     KKTPYL"
BASE COUNT          655 a          362 c          269 g          678 t
ORIGIN      
        1 gttccttctt tgcgggaaag aaaatgaata tcatccatca aaagcaggtc aacttttctg
       61 aattttgctc tgaattccgg cattttatta ctgcgaatgc catcaatcat ttcattggta
      121 aatgcctcgg aagttatata gtaaatggaa cagtttcgtc cttcttttaa aatataattt
      181 ccaattgcct gcatcagatg ggtctttccc attccgcttt caccataaat aaaaagagga
      241 ttataggtat aaccaggcga ttctgctacc gcttttgctg cactataagc aaaattgtta
      301 tttttaccca ctacaaattc atcaaaagta tagcgctcat ttaatcttac attggcacta
      361 acccgcttaa tactataatc ctgaacatcg ttttctttat tgtgattacg gtggggctga
      421 gttataaatt gaacatcata cttccgacca taaagaccat aagaaatttc tcctaaaacc
      481 tctttgtaat tctgattcag ataattagcc gaaaattgag ttgccactct aatcatcaat
      541 tccctgtcat tcatatctac taatcttgta tctgaaaacc atgttttgaa actttgagga
      601 tttatatttt cctccagttt atccaatatg ttctgccaaa tatcctgatc cataagtcac
      661 tttcctgtaa aatttattta tccacaaact gtatgctgaa agcccagtaa cagttttatg
      721 attactatct tattcaaatt taataagtta tgccgataaa atagacatcc agtcaactac
      781 ctgaatccga tttatccaca acttatccac aacaccttga taaatttctt tcttttcttt
      841 actttaagct gttttcaatt ttgttaaaaa aaccatcctc accaaaaaaa tatttcactc
      901 tgatttgtca agcttctaaa atctaaaatt aaaatagctt agagaagaat taaaattgat
      961 aattccctca tttagaaaaa agttagaatt ctaatcccga tttttctata actattttta
     1021 tagaactatc tttcctaatt atatgctatt atcgtttcca aaaaattttt gtatttttat
     1081 accgaaagca ccaagaataa cggaaacaca tatattttag ggttttttta caaagagaac
     1141 aagagaaaaa gagataggtt aagatttagg accttatatt gtaaagagaa caagagccga
     1201 tatcagttct tgaaggaaaa gccctttatc attccgatga aaacagaaat acacagaata
     1261 gaagaaaata tttatgcttt tttaagagca aaactatata atcagggaag aaaatagaac
     1321 gccgataagc ggatccacaa ggttaatagg attttttctt gaaatatagt ccactatttt
     1381 tagaacagct catcttcttt aatctccatt ctcttaatag cattgcttac tcattacgga
     1441 ctcattacgg atggcattag caatgggtaa gcaatgagta agcaatgcta ttagggaaga
     1501 aggggaaata aaaaaataga ggcggcaaat ttttccttgc cacctctatt attatatctg
     1561 taaaaatggt ttgcggtttt aaatatacag cgtaattttc tttaagtaac ctttaatttc
     1621 taaggcaaag cagtatttat catctcaatt ccatatatta ttgttaataa agcgtatcat
     1681 aaataggtga atcccaaatg ttggtgtaaa ttccaactct ttgtttatca ggttctttga
     1741 gtacattaca agtaaggagt ttttttgcta atagtatcaa atgtgttttt aaccttatca
     1801 tcatcaaaat aggcactgct atccgtattg attcttttcc aaagctccag aattctctga
     1861 ttcagatgca ttactttcaa ttcatctacc aaagtcatat cgcctccttt acctatatat
     1921 tggatttcct aacccgattc tatccactca gatactgcct taaa
//
LOCUS       DAOPFL010000200         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_83949_length_1940_cov_100.552785, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000200 DAOPFL010000000
VERSION     DAOPFL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83949_length_1940_cov_100.552785"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1382
                     /locus_tag="PLG69_07945"
     CDS             87..1382
                     /locus_tag="PLG69_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPI43474.1"
                     /translation="MQVEFNQLNAVTKEINLTIPSEEVDKEYEKYLKKSAQEISVPGF
                     RKGKAPLNVVERMYEDKIRNYFYEYYIDEVFSKVVKENEIKYLHYPEVKDVQWEKGND
                     MTIKIEIEHEPVLEFKQIEGLTVPYKPIVLEEEVNNFIKELQKSNCRIIDVETARETD
                     NVNVEITFQHNNETFTRTGSFFAGTKHNLDMLPELLGLKIGDKTKVKLTGREIINSTQ
                     DYKLPLDNDAEYDCELMVNSISRIEYPELDDEFAKDMEFDSLEDMKAKISDDLKLKIE
                     HSNIDIENEAIITKLFTDNEFALPKKTLSYLAEEQVKDIKNDNFMQYYYERYYITFAY
                     ALLSLYITNNLLRLMPIELTDEMKEEYINHLAIVKDMSSEAYKEKYKDDISSENFARD
                     AQRYFVLRKIAQTCEFVIKEEPEENSYPESEIETIKEEQ"
     gene            1379..>1940
                     /gene="clpP"
                     /locus_tag="PLG69_07950"
     CDS             1379..>1940
                     /gene="clpP"
                     /locus_tag="PLG69_07950"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPI43475.1"
                     /translation="MSFVPIVIEQVGRTERAYDIYSRLLKDRIVFVGGVIEDNLANTV
                     VAQLLHLEGEDPERDIYMYINSPGGIISAGLAIYDTMQYIKPRVSTICIGQAASMAAV
                     LLAAGAPGKRSALPNSRIMIHQPMGGAQGQATDIEIQAKEILYLRGRMNEILHKHSGQ
                     SIEKIMEDTERNYFMSAEEAMEYGIID"
BASE COUNT          727 a          273 c          388 g          552 t
ORIGIN      
        1 ttaggatcca gtgaggttaa actcataggg gttcaagtcc cctctttcgc accagttttt
       61 ggatgatata aaattaggag aataaagtgc aagtagaatt caatcaatta aatgcagtaa
      121 caaaggaaat taatcttaca attccttccg aggaagttga taaggaatac gaaaaatatt
      181 tgaaaaagtc agcccaggaa ataagcgttc ctggtttccg gaaaggtaaa gcgcctctga
      241 atgttgtaga acgaatgtat gaagacaaaa tccggaatta tttttatgag tattatatag
      301 atgaggtctt cagtaaagtt gttaaagaaa atgagataaa atatcttcat tatccggaag
      361 taaaagatgt tcaatgggaa aaaggtaatg atatgacaat caagattgag attgaacatg
      421 aacctgttct tgaattcaaa cagatagaag gtttaactgt tccatataaa ccgatagtgt
      481 tggaagaaga agtaaacaat tttattaaag aattacaaaa atcaaattgc cggataattg
      541 atgtagaaac agcaagggaa accgataatg taaatgtaga gattactttt caacataata
      601 atgaaacatt taccagaaca ggcagttttt ttgctggaac aaaacataac ctggatatgc
      661 tgccggaact actgggtcta aaaattggcg ataaaactaa agtaaaatta acaggtagag
      721 aaattattaa ttctactcag gattataaac ttcctttgga taatgatgca gaatacgatt
      781 gcgaattgat ggtgaatagc atttccagaa tagaatatcc ggaactggat gatgaatttg
      841 ccaaggatat ggaatttgat tctctggaag atatgaaagc gaaaatcagt gatgatttaa
      901 aattgaaaat tgaacattca aacatagata tagaaaacga agctattata accaagcttt
      961 ttactgataa tgagtttgct ctccccaaaa aaactctatc ctatcttgcg gaagaacaag
     1021 ttaaggatat taaaaacgat aattttatgc agtattatta tgagcgctac tatattactt
     1081 ttgcttatgc tctattgtca ttgtatatta cgaataatct attaaggtta atgcctattg
     1141 aacttactga cgaaatgaaa gaagaatata taaaccactt ggctattgta aaagatatga
     1201 gctccgaagc atataaagaa aaatataaag atgatatttc ttctgaaaat tttgcccgtg
     1261 acgctcaaag atactttgtc ttaagaaaaa tcgctcaaac ctgtgagttc gttattaaag
     1321 aagaacccga ggaaaatagc tatccggaat ccgagataga aacaattaag gaggaacaat
     1381 gagttttgtc cctatagtta tagaacaagt aggcagaaca gaacgtgctt atgacattta
     1441 ttcacgtttg ctgaaagaca gaattgtctt tgtcggtgga gtaatagaag ataatttggc
     1501 aaatactgtt gttgcccaat tacttcattt agaaggtgaa gatcctgaac gggatattta
     1561 tatgtatata aacagtcccg ggggaattat ttctgcagga cttgcaattt atgatacaat
     1621 gcaatacata aaaccccgcg taagtactat ttgtattggt caggcagcaa gtatggcagc
     1681 tgttttattg gcagcaggtg ctcctggaaa aagaagcgct ctgccaaata gcagaattat
     1741 gattcaccaa ccaatgggag gagcacaagg tcaagcaacc gatattgaaa ttcaggcaaa
     1801 agaaattctc tatttacgtg gtaggatgaa tgaaatccta cataaacata gtggtcagag
     1861 cattgaaaaa attatggaag atactgaacg gaattatttt atgagtgctg aagaagcaat
     1921 ggaatacggt attatagatg
//
LOCUS       DAOPFL010000201         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_86062_length_1907_cov_100.581533, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000201 DAOPFL010000000
VERSION     DAOPFL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86062_length_1907_cov_100.581533"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1281
                     /locus_tag="PLG69_07955"
     CDS             157..1281
                     /locus_tag="PLG69_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43476.1"
                     /translation="MKKKIVYALLIVALLGIFSCDRFDHNFKPLEQVDFNAELFSPLE
                     EAFNTASAEDLSTVMAFYAEDYKHYGITKSEWEAILYNLLSGINNPVIDVTLITATLQ
                     NETNALANWRLTISDSRKNVIADSLYTGERLRKENGKWLLRGNQCGCDPSAPTQKVIV
                     EYVTNVGCSYCPAVENLLHDLSSQYGNDFIYLTHQLSGPVAFNDPLYAYYSAYSAPVS
                     IIQGKHKLVGGNQETLSQYPYIVQSELELLSQMDYSVLNATVEGINISGSVKLTPLST
                     SFNQEELILNLAVIDLVSTALNAEGEHLTNVVIGRKRIDISEVDLSNPVAFEFSANVT
                     IPEDAALVIFAQKTPATFANNATIYSGLQYPLAVKKGGSQ"
     gene            1278..1769
                     /locus_tag="PLG69_07960"
     CDS             1278..1769
                     /locus_tag="PLG69_07960"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPI43477.1"
                     /translation="MKKPIACLLLLLLVSFAMADMMPDFKLPDATGKNVILQELLGKG
                     PVLIDFWADYCKPCKDAMPQLNTLAEKYDSLTVVMISIDAPKNLPKAKNYLKSKNYKF
                     ITLFDSEKTLAKKLGVTNPPHTFILNKTGEIVYSHIGYEPGVENEYEMHIRNLLGLKT
                     EAE"
BASE COUNT          599 a          355 c          375 g          578 t
ORIGIN      
        1 ataaaaaaac acggaagagc aggcgttcct tttaatgctt tatatattcc gggtgcagaa
       61 ccccatattt ttggcgaact gctttccaaa gatgaggtaa taaatgctct taataaaatt
      121 cctgcccaag cagaatagat aaagttagga aaaaagatga aaaagaaaat tgtttatgcc
      181 ctgttaatag ttgcactttt aggcatattt tcctgcgatc gctttgacca taattttaag
      241 ccactggaac aggtggattt taatgcggaa cttttcagtc ctttggaaga agcatttaat
      301 actgccagcg ctgaagattt atctactgtg atggcttttt atgcagagga ctataaacat
      361 tatggcatca ctaaaagcga gtgggaagct atcttataca atctgctttc cggtataaat
      421 aatcctgtta ttgatgtaac tttaataaca gctactctgc aaaatgaaac caatgctttg
      481 gcaaactggc gtttaaccat tagtgacagc cgtaaaaatg ttattgccga tagcttatat
      541 accggagaac gcttaaggaa agaaaacgga aaatggctgc tgcgaggcaa tcaatgtggc
      601 tgtgatcctt ctgctccaac tcaaaaagtt atagtggaat atgttactaa tgtgggttgc
      661 agttactgtc ccgctgtgga aaatctttta cacgatttaa gctctcaata tggaaatgac
      721 ttcatctatt taacccatca gctttccggt cccgttgctt ttaacgatcc tctctatgct
      781 tattatagcg catacagtgc tcctgtttct attattcagg gaaaacataa actggtaggt
      841 ggaaatcagg aaactttaag ccaatatcct tatatcgtgc aaagtgaatt ggaattatta
      901 agccagatgg attacagtgt tctaaacgct accgtagagg gaataaatat ctccggaagc
      961 gtaaaactta ctcctctatc cacatccttc aatcaggaag aactgattct gaatctggca
     1021 gttattgatt tggtttccac agcgctaaat gctgaaggag agcatcttac caatgtagta
     1081 ataggcagaa aacgcattga tataagcgaa gtggatttaa gtaaccctgt tgcctttgaa
     1141 ttcagcgcta atgtgacaat tcctgaggat gccgcattag tgatttttgc ccaaaaaaca
     1201 cctgccacct tcgctaataa cgctactata tatagtggct tacaatatcc tcttgctgta
     1261 aaaaaaggag gttcacaatg aaaaagccga ttgcctgttt gctcctgctg cttttggttt
     1321 cttttgctat ggcagatatg atgccggatt ttaagcttcc cgatgcaaca ggaaaaaatg
     1381 ttattctaca ggaacttctg ggaaagggtc ctgtgctgat tgatttttgg gctgattact
     1441 gtaaaccttg taaagatgct atgccacaat taaataccct ggcagaaaag tatgattcgc
     1501 taacggtggt aatgatttct attgatgctc ctaaaaatct gcccaaggca aaaaactatc
     1561 tgaaaagcaa gaattataag tttataactc tgtttgattc cgaaaaaacc ttggctaaaa
     1621 aactgggagt tacaaaccct ccgcatacct ttattctaaa taaaaccgga gaaattgtct
     1681 attctcatat tggctatgaa cctggtgtgg aaaatgaata cgaaatgcat atccgtaatt
     1741 tattgggact taaaaccgaa gcggaataac gatagtttca cgcagattac gcagattacg
     1801 cagatttttt atttgaggtt ttaatctatg attataactt ggttgatgag ggaaagtcag
     1861 gactcgttac tgccgaaata acctttacac ctttcacgaa ttcattt
//
LOCUS       DAOPFL010000202         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_86213_length_1905_cov_127.908649, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000202 DAOPFL010000000
VERSION     DAOPFL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86213_length_1905_cov_127.908649"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLG69_07965"
     CDS             complement(<1..814)
                     /locus_tag="PLG69_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43478.1"
                     /translation="MTYHNPDLEFETLVAQIGKRCHSDLGKEQASLLQPLSDLGEIIK
                     SQKLVAEIQEQLLRGRDYDFSGLTNLQPLFEDSRNTLFAFEEFSAVYANNLITEEILA
                     QLKLWEEFPEITLYLKKLTSFSFLNSRFLEIFDKDGEILDTASKELSTIRKRANSLRM
                     QIQKTMQALLSDSSLEHYLQDKYVTQREDRYVLPVKENAVPFVNGIVQSHSGSKSTVF
                     IEPMSVVPLNNELQLIFQQEKQEIYRIFSAYTSAIKAEKKGLLRNQKILAWLD"
     gene            complement(976..1530)
                     /locus_tag="PLG69_07970"
     CDS             complement(976..1530)
                     /locus_tag="PLG69_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPI43479.1"
                     /translation="MGFIDWIILAVLILFTFFGWRKGLLASLVQLGGYILTFFLVGHY
                     YPLVQRNLMQKFHFAKWLATIVSVVLIIVLIVVIVRLVIYILERFLRALKLSAVNHTL
                     GAVLGIINGLLVVIILTVVLDFIPKLSTPLKNGEKHRVYAGVDLVKEEIFTKLKLTER
                     TKLIKMPKLPFAEKLNGEKVKSEK"
     gene            complement(1622..1828)
                     /gene="rpsU"
                     /locus_tag="PLG69_07975"
     CDS             complement(1622..1828)
                     /gene="rpsU"
                     /locus_tag="PLG69_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPI43480.1"
                     /translation="MPTVVAKENESFDLLLKRFKKKCEKAAILSEIKKNQAYEKPSVR
                     KKREANVARRKMLKMQRRMQRYLK"
BASE COUNT          588 a          356 c          350 g          611 t
ORIGIN      
        1 aatccagcca agccagaatt ttctgattgc gtaaaagtcc tttcttttct gctttgatag
       61 ctgaagtata tgcactgaaa atacgataaa tttcctgctt ttcctgctga aaaataagct
      121 ggagttcatt attgagaggt acaacagaca ttggttctat aaaaacggta gatttactgc
      181 cggaatggct ttgaacgatg ccatttacga aaggaaccgc attttcttta acgggcagaa
      241 cataacggtc ttccctttga gttacatatt tatcttgcaa gtagtgttct aaactactgt
      301 cggaaagtaa tgcttgcatt gttttctgaa tctgcattct taaggaatta gctcttttgc
      361 ggatagttga tagttctttg gaagcggtat ccaaaatttc accgtcttta tcaaaaatct
      421 ccaaaaacct agaattgaga aaggaaaagg aggttaattt ctttaggtaa agagtaattt
      481 ccggaaattc ctcccaaagc tttagttgag ccaaaatttc ttcggtaatg aggttattgg
      541 cataaacagc agaaaattcc tcaaacgcaa aaagagtgtt tcggctgtct tcaaaaagag
      601 gttgcagatt agttaaacca ctaaaatcat aatctctacc ccgcaaaagt tgttcctgga
      661 tttcggctac caatttctgg gatttgatta tttctcccaa atcggatagg ggctgtaata
      721 aagatgcctg ttctttacct aaatcactat gacagcgttt gccgatttga gcaacgaggg
      781 tttcaaattc aaggtcggga ttatgataag tcataaatta gatagatagc gagatggaga
      841 agtgagagga ctttgtccaa tgagagttaa aacagtgaga agaatttggg ggataggtaa
      901 tagttatttt ggcagccacg actcctgact taactttgca ccgaaaatta tcactttgtt
      961 tcactttttc acctttcact tttcactttt taccttttct ccgttcaact tctccgcaaa
     1021 aggcaatttc ggcattttaa tcaattttgt tctttctgtc agtttcagtt tggtaaatat
     1081 ttcttcttta acaaggtcta caccagcata aacgcgatgt ttttctccat ttttcaaagg
     1141 ggtagaaagt ttgggaataa aatccagaac aacggttaaa ataataacta ccagcaaacc
     1201 gttgataatt cctaaaacgg cacccagagt atggttaaca gcagagagtt ttaaagcgcg
     1261 taaaaacctt tccaggatgt aaataacaag tcgcacaatc accacaatta aaactatgat
     1321 taaaacaacg gaaacaatag ttgccagcca tttggcaaag tgaaattttt gcattaaatt
     1381 gcgttgcacc agaggatagt aatgacccac cagaaagaaa gtgagaatat accctccaag
     1441 ctgaactaaa cttgccagca gacctttacg ccaaccgaaa aaagtgaata agatcaggac
     1501 tgccaggatg atccagtcaa taaatcccat tttttccccg cttaaaaaag cttacaggga
     1561 ataatcttcc ctgtagctta tattcttgta attagatgaa ttctcttcgt taagaggagt
     1621 cctatttcag atagcgttgc atccgacgtt gcatttttag cattttgcgt cgggcaacat
     1681 ttgcctcgcg tttcttacga acgcttggtt tttcgtatgc ctgattcttt tttatctcgg
     1741 aaagaatagc tgctttttcg cattttttct taaatcgttt taataaaaga tcgaagcttt
     1801 cattttcttt tgctacaact gtaggcaaat aaatcaccac ctttatttta tattttttct
     1861 aatgccatat ttttaaatgt tgttatgctg tcaagggaga aaaat
//
LOCUS       DAOPFL010000203         1900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_86520_length_1900_cov_96.570190, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000203 DAOPFL010000000
VERSION     DAOPFL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86520_length_1900_cov_96.570190"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLG69_07980"
     CDS             <1..541
                     /locus_tag="PLG69_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HPI43481.1"
                     /translation="LSHAPRRNQTVMMLIDAFLPEGITRLSVDSIFMMPHLGVLSEIS
                     TKAATEVFRKDCMIYLGTCVAPVGKGKIGKPALYAKLELPDGSVFEEDIPFGEMRLIP
                     CEIGQVAKATLKTHSGLILDADRKKELTVDLHGGKVGIVLDTRGRKPFVLPADKNERI
                     SALKKWMKVLNAYPEHILS"
     gene            554..1684
                     /locus_tag="PLG69_07985"
     CDS             554..1684
                     /locus_tag="PLG69_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43482.1"
                     /translation="MGQAYSAGLTVTDNTILRKERILPLKGQVLVKKGDKVKAEDVVA
                     ETLLPGKVIPFNLANKLGVTPQQLVNYIKIKPGDKITQKTVLAETKGLFGLGIMKTEI
                     LSPIEGEVENISSVTGQILLREPRIPVQVKAFIDGLVTDVIEEEGVIIENKSAYIQGI
                     FGLGGETFGELKMIAESPDDELDVAKIDASCAGKIIVAGAFAPYHAIDIARKNGVKAI
                     ITGGIDDQDIKKLLGYDIGVAITGHENIGITIVCTEGFGRINMAQKTFNLLKHFEGYK
                     TSIHGRTQIRAGVIRPEIIIPLEFEEQELVAKEVSMPILEIGTVIRIIRQPHFGHIAK
                     VTALPEELTKVESETLVRVLEAEFENGTKIIVPRANVEVIES"
BASE COUNT          611 a          340 c          423 g          526 t
ORIGIN      
        1 tttatctcat gctccacgca gaaatcagac cgtgatgatg ttaattgatg cttttttgcc
       61 tgaaggaata acccgtcttt ccgttgatag catttttatg atgccgcatt tgggagtcct
      121 atctgaaatc agcaccaaag cagctacaga ggttttccgc aaggactgta tgatttattt
      181 gggaacttgc gtagcccctg taggaaaagg aaaaattggt aaaccagctc tctatgccaa
      241 actggagctt cctgatggct ctgtttttga agaagatata ccctttggcg aaatgcgttt
      301 aatcccttgt gagattggac aggttgccaa agcaactctg aaaacacata gtggcttgat
      361 tttagatgca gatagaaaga aagaactgac cgttgatctg cacgggggaa aagttggcat
      421 cgttttagat accagaggtc gtaaaccttt tgttcttcct gcagataaaa atgaacgcat
      481 ttctgccctt aaaaagtgga tgaaagtcct gaatgcatac ccggaacata ttttgtcata
      541 ggaggataaa aaaatgggac aagcatattc tgccggttta accgttaccg ataatacaat
      601 cttacgcaaa gaacgcattc tgcctttgaa aggacaggtg ctcgtgaaaa aaggcgataa
      661 agtaaaagct gaagatgttg ttgccgaaac cttactccca ggtaaagtta tacctttcaa
      721 tcttgccaat aaacttggag ttacccctca acaattagtt aattacatta aaattaaacc
      781 gggtgataaa atcactcaaa aaaccgtttt ggcggaaact aaaggacttt tcgggctcgg
      841 cataatgaaa acggaaattc tttcccctat tgaaggagaa gtggaaaaca tttcttccgt
      901 aacaggccaa atcttactgc gtgaaccccg cattcctgtt caggtaaagg catttattga
      961 tggattggta accgatgtta ttgaagagga aggagtaatc attgaaaata aaagtgccta
     1021 tattcaagga atttttgggc tcggaggaga aacctttggc gaattaaaaa tgatagcaga
     1081 aagccccgat gatgaattgg atgttgccaa aattgatgca tcctgtgctg gtaaaataat
     1141 tgttgccggt gcttttgcac cctatcacgc aattgatata gcccgaaaaa atggcgtaaa
     1201 agctattatt acaggtggta tagacgatca ggatattaaa aaattgctcg gctatgatat
     1261 tggagtagct attacaggac acgaaaatat cggcattact atcgtttgca cagagggttt
     1321 tggcagaatt aatatggcac aaaaaacctt taatttatta aaacattttg agggttataa
     1381 aacctctatc catggcagaa ctcaaattcg tgccggagtt attcgtccgg aaattattat
     1441 tccgctcgag tttgaagaac aggaactcgt tgctaaagaa gtttctatgc ctattttgga
     1501 aatcggaacc gtaatcagaa ttatccgtca accccatttt ggacacattg ccaaagtgac
     1561 tgctctgccg gaagaactta ctaaagtgga atcggaaaca ttggttcggg ttttggaagc
     1621 ggaatttgaa aatggcacaa aaattatcgt tccacgtgct aatgttgaag taatagaatc
     1681 ctgagcaaac ataatacctc agaaaaaaag cccgtcaggg caactcattg atagcgatgg
     1741 tggcgtaaac ccctcggaaa ataaagttta gagggttgag aaggtttaga gggttgaaaa
     1801 gttttaaaag gttgataagt tttagaaggt tgataaggat tagatacatt aaagcacgtc
     1861 agggcgatac tttcatagcg atgggtgtgt aaacccctcg
//
LOCUS       DAOPFL010000204         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_86939_length_1894_cov_107.179445, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000204 DAOPFL010000000
VERSION     DAOPFL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86939_length_1894_cov_107.179445"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PLG69_07990"
     CDS             complement(<1..1186)
                     /locus_tag="PLG69_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPI43483.1"
                     /translation="MFCLVLLILPIFAQSVKFSISPEVLKPGEKGVIKATLLIPEGKK
                     QTVNPQNPEYFYLEASHPDLILGKVIYPKPTQVVSEEEWNYHPQVTLTLPFTVKQTAK
                     TGNKQIEVLLSYNLCYETGMCDPPEEATAILSIKIAENEPIPAAVSENEEETPNTIEQ
                     KETIPIEKSPIPDKKPETSHPLGEILKYILFAFLGGIILNITPCVLPILPIRIMSIMN
                     QAQKDKTKVLNHTLIYALGVLISFAVMAGIFIALQKAGESVGWGLQNQNPGFVVTLMS
                     IVFVFALSLLGVFEITVPGMNTANKATSRGGYSGSFFGGIFAFLMAISCTGPFLGAAL
                     PFALALPPVLMLIFFLTIGLGFAFPFLLIGFFPKALKIIPKPGNWMIIFKEVMGFVLL
                     IIV"
     gene            complement(1442..>1894)
                     /gene="frr"
                     /locus_tag="PLG69_07995"
     CDS             complement(1442..>1894)
                     /gene="frr"
                     /locus_tag="PLG69_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPI43484.1"
                     /translation="ILDDIKINYYGLPTPIKQLCNISIPEARMIVIQPWDKTTLADIE
                     KAILAANIGITPENDGNVIRLPFQPLTEEKRRDIVKNIKKISEDTRVAIRNIRRDAND
                     IVKKEKKDSEISEDEEKKQLKELQDLTDDWIKKIDEAEKAKEKEIMEV"
BASE COUNT          581 a          371 c          346 g          596 t
ORIGIN      
        1 aaacaataat aagcaaaaca aagcccatca cttctttgaa aataatcatc cagttgcctg
       61 gcttgggaat tattttcaac gccttaggga aaaagccaat cagcagaaat ggaaacgcaa
      121 aaccgagccc gatagtaaga aagaaaatca gcattaaaac aggtggcaaa gccaaggcaa
      181 aaggcaaagc cgcacctaaa aaaggtcccg tgcaagatat cgccatcaaa aaggcaaaaa
      241 tacccccaaa aaaggaaccg ctatagcccc ctcgggaagt tgccttatta gccgtattca
      301 ttcccggaac agttatttca aagaccccta aaagtgacaa agcaaacaca aacacaattg
      361 acatcagggt taccacaaaa ccaggatttt gattttgcaa accccaacct accgattcac
      421 ctgccttttg caaagcgatg aaaattcctg ccattacggc aaaggaaatc agcaccccca
      481 aagcgtaaat caaagtatga ttcagcactt tggttttatc tttctgcgct tgattcatta
      541 tactcataat tcttatcggc aaaatgggta aaacgcaagg tgttatattt aatataattc
      601 cgcctaaaaa ggcaaaaaga atgtatttta gtatttcgcc aagaggatgg ctggtttcag
      661 gttttttatc aggaatcggg gatttttcaa taggaatggt ttccttttgt tctatagtgt
      721 ttggggtttc ctcttcattt tccgaaacag cagctggtat tggttcattt tcagctattt
      781 taatagaaag gatagctgtt gcctcttcgg gtggatcaca cattcctgtt tcgtagcata
      841 gattatagct cagcagaact tcaatttgtt tgttgcctgt tttagctgtt tgtttcaccg
      901 taaaaggcaa agttaaagtt acttggggat gatagttcca ttcctcttcc gaaacaacct
      961 gtgtcggttt cggataaata actttaccca aaataaggtc tggatgactt gcttccagat
     1021 aaaaatactc cgggttttgg ggattaacgg tttgtttttt cccttcagga ataagaagag
     1081 tggctttaat aacgcctttt tctccaggct tcaagacctc aggagaaata gagaatttaa
     1141 cgctctgtgc aaaaattggc agaatgagca acactaaaca gaacattaac aacagcttaa
     1201 gacatctatg catcagcaat atccttgtta catatttggg tacttatagt aaatgataat
     1261 gcagaaaaca ttccagacaa gacaactatt gtgggaatga aaaataaaga gtgtatgagg
     1321 gggttcaaaa aaatagcgga ttataattaa agaaaaatcc aattaatcca gtatatccag
     1381 ttatctgtgt tctatatctt cacgctctta aaatctcttt ttctctttgt aaaaaaaccc
     1441 tttaaacttc cattatttct ttttcttttg ccttttccgc ctcatctatc tttttaatcc
     1501 aatcatctgt aaggtcttgc agttctttca gttgcttctt ttcttcgtct tcacttattt
     1561 cgctgtcttt tttttctttt ttaactatat cattggcatc gcggcggata ttccggatag
     1621 caactctggt atcttccgag atttttttaa tatttttaac tatatctctg cgtttttctt
     1681 cggttaaggg ctgaaaaggc aggcggataa catttccatc gttttccgga gtaatgccaa
     1741 tattggcagc taaaatggct ttttctatat ctgccagggt tgttttatcc cagggctgaa
     1801 taacaatcat tctggcttcg ggaatggaaa tgttgcagag ttgtttaatc ggtgtaggta
     1861 gaccgtaata attgatttta atatcatcca aaat
//
LOCUS       DAOPFL010000205         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_88367_length_1872_cov_95.468354, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000205 DAOPFL010000000
VERSION     DAOPFL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88367_length_1872_cov_95.468354"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLG69_08000"
     CDS             complement(<1..800)
                     /locus_tag="PLG69_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43485.1"
                     /translation="MVKKFLGLIICLMSIGYLFSAEKYVTIEVDTVKTYPLTISVAEA
                     RIKTLQEVRQVALERALPQDISLTTFISSFSVEKNNFYDEQVASSVFMNCCSSGRIIE
                     EKILEDYPSFPKKSNVFEYRMKYRATILPLKTIYNSELDLKVQLSETLLKNNEQFTLE
                     LTPNQDGYLYIFDFLPDNSVLVVYPTLHYPESLIIKDTAWKQTLTAVTSPDKKHSIET
                     LYFVFSEVPISGWENFKSNSNFTELIFSAGEESFILFQKWLGRSDPNKR"
     gene            complement(794..>1872)
                     /locus_tag="PLG69_08005"
     CDS             complement(794..>1872)
                     /locus_tag="PLG69_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPI43486.1"
                     /translation="ILGKDIFLLPGSNAENQFDQLSAGEYKDLHYTLVTAKTATNLNL
                     TLDILEAHTQFNKLNQPLNLAFNRQERTAESIIISGKERKINIENAPALSIDVEQNIP
                     VLSKADNKKWGVIFGIENYRNVSSVPFARRDAEFMKEYFNKVLGIPTENIYFQTDESA
                     TLSEFKTVFDPGGWLAKNAGKPGNEIYIYYSGHGAPDPAGNKAYLLPADGNPNYAAIS
                     GYGLDQLYANLAQLKVKQITLFLDSCFSGANRDNETILASARPVFITTTIPSVASNIA
                     VFSAATGNQISSGYSDKQHGLFSYYLMKGLRGEADSNKDNKLTQKELNDYLSEQVNTQ
                     ARRMGREQEPQLQSAEPERVILQW"
BASE COUNT          563 a          390 c          306 g          613 t
ORIGIN      
        1 cgtttgttag gatcgcttct tcccagccat ttctgaaaca aaataaagct ttcttctcca
       61 gcactaaata tcaattcagt aaaattactg ttacttttaa aattttccca cccggaaatc
      121 ggcacttccg aaaatacaaa gtaaagcgtt tcaatgctat gctttttatc tggacttgta
      181 acagctgtta gagtttgttt ccaggcagtg tcttttataa ttaaagattc cggataatgc
      241 aaagtaggat aaactaccaa aacgctgtta tccggcaaaa aatcaaaaat atacagatat
      301 ccatcctgat taggtgtaag ctctaaggta aactgttcgt tatttttaag caatgtctcg
      361 ctcaattgaa ctttcaggtc cagctccgaa ttataaattg tctttaaagg aaggatagtt
      421 gccctatact tcattctata ctcaaaaaca ttgctttttt tagggaaaga aggataatcc
      481 tccagaattt tctcctctat aattcgcccc gaagaacaac agttcataaa aacgctggaa
      541 gcaacttgct catcataaaa attattcttt tccacactga agctggaaat aaaagtggta
      601 agcgaaatat cttgcggcaa tgctctttct aaagccacct gacgaacttc ctgcaatgtt
      661 tttattcttg cttcggctac agaaatagta agaggatagg tctttacagt atccacttct
      721 atggttacat atttctctgc gctaaacaaa tagccaatac tcattaaaca aattatcagc
      781 cccagaaatt tttttaccat tgcaaaataa ccctctccgg ttctgcactt tgcaattgtg
      841 gctcctgttc gcgtcccatt ctgcgtgcct gagtatttac ctgttcactc agatagtcgt
      901 taagttcttt ttgggtaagc ttgttatctt tattgctatc tgcctcgccc cgtaaacctt
      961 tcatcaaata ataggaaaat aagccatgtt gtttgtcgct atagccagaa gatatctgat
     1021 ttcctgttgc tgctgaaaag acagctatat tagatgcaac cgaaggtatc gtagtagtga
     1081 tgaaaactgg tctggcactt gctaaaatag tttcattatc gcggtttgca cctgaaaaac
     1141 agctatccaa aaacaaagta atctgcttca cttttagctg cgctaaattg gcataaagct
     1201 gatcaagacc gtatccggaa atagctgcat aattgggatt tccatccgct ggcaataaat
     1261 aagctttatt ccctgctgga tcgggagctc catgtccgct gtaataaata taaatttcat
     1321 tgcctggttt gccggcattt ttagctaacc agccacccgg atcaaaaacc gtcttaaatt
     1381 cactcagagt tgccgattca tctgtttgaa agtaaatatt ttcagttggg attcctaata
     1441 ccttattaaa atactctttc ataaattctg catctcgcct ggcaaaagga acactactca
     1501 cattccgata attctctatc ccaaaaatca ctccccactt tttattatct gccttagata
     1561 aaactgggat attttgttcc acatcaatag ataatgctgg tgcattttca atatttatct
     1621 ttctttcttt acccgatatg ataatggact ccgccgttct ttcctgacgg ttgaaagcca
     1681 gatttagagg ttgattaagc ttattaaatt gagtatgagc ttctaagatg tccaaagtta
     1741 aatttaaatt cgtagctgtc ttggcagtta ctaaagtata atgtaaatct ttatattctc
     1801 cagctgacaa ttgatcaaac tggttttctg cattgctgcc aggtaaaaga aatatatcct
     1861 tacctaaaat ta
//
LOCUS       DAOPFL010000206         1868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_88622_length_1868_cov_123.971870, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000206 DAOPFL010000000
VERSION     DAOPFL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88622_length_1868_cov_123.971870"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLG69_08010"
     CDS             complement(<1..278)
                     /locus_tag="PLG69_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPI43487.1"
                     /translation="MSKKRKPEWTPVNSGKEFASEIVTGTKKLKTKTAPPLKEKNYDL
                     KTLVKGVREGNRTLLAKAITLIESNAEKHFLPAQELIKELLPYSGNSIR"
     gene            complement(271..>1868)
                     /gene="scpA"
                     /locus_tag="PLG69_08015"
     CDS             complement(271..>1868)
                     /gene="scpA"
                     /locus_tag="PLG69_08015"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPI43488.1"
                     /translation="NGTIQNDILKEYMVRNTYIYPPEPSMRIIADIFSYTAKNMPKFN
                     SISISGYHMHEAGATADLEMAYTLADGLEYVRTALKAGLDIDVIAPRLSFFWAEGMNY
                     FMEVAKLRGARVLWAKIIKQFNPKNPKSMALRTHSQTSGWSLTEQDPYNNITRTCIEA
                     LAATMGHTQSLHTNSLDEAIALPTDFSARIARNTQLYLQNETGICRVIDPWAGSYYVE
                     FLTDALMKSAWKHIIEVENLGGMAKAIETGLPKMRIEEAAARRQAKIDSGADIIVGVN
                     KYPPETEIPIEILEVDNSAVRDTQIARLEKLRRERNSEEVEKALQAITKSAETGEGNL
                     LELAIDAARKRATLGEISYAMEKVWGRHQEAIRLINNVYSSEYTKMDEIEQVRVLTDK
                     FAQQEGRRPRILVAKMGQDGHDRGAKVVATAFADMGFDVDVGPLFQTPEETARQAVEN
                     DVHIIGISSLAGGHKTLLPKLMEELKKYGREDIMVTVGGVIPPQDYEFLYQNGASFIF
                     GPGTKLTESAKQIMEKLLARGNE"
BASE COUNT          475 a          432 c          340 g          621 t
ORIGIN      
        1 cgaatggaat ttccggaata aggcagaagt tctttaatca gttcctgtgc aggtaaaaag
       61 tgcttttcgg cattgctttc tattaaagtt attgctttgg caagcaaagt cctattccct
      121 tcccggactc ctttcaccag cgttttcagg tcataattct tttctttaag cggaggtgcg
      181 gttttagttt tcagtttttt tgttcctgtc actatttcgc tggcaaattc ttttccgcta
      241 ttaacaggcg tccattccgg tttgcgtttt ttactcattc ccgcgtgcca aaagcttttc
      301 cataatttgt ttggctgatt cagtcagttt agttcccggt ccaaaaataa aagaggcacc
      361 attttgatag agaaactcat aatcctgcgg aggaataacc cctccaacgg ttaccattat
      421 atcttccctg ccatattttt tcagctcttc catcagcttg ggtaaaagtg ttttatgccc
      481 tcctgccaga gaactgatac caattatatg cacatcattt tccactgcct gacgcgctgt
      541 ttcttctggg gtctgaaaca gaggacctac atccacatca aaacccatat cggcaaaggc
      601 agtagctact actttagcac cacgatcatg accatcctga cccattttgg caactaaaat
      661 acgaggtctt ctgccttcct gttgagcgaa tttgtcggtt aaaacccgaa cttgttcaat
      721 ttcatccatt tttgtgtact cactgctata aacattatta attaaacgaa tggcttcctg
      781 gtgacgaccc caaacttttt ccatagcgta ggatatttca cctaaggtag ctctttttct
      841 ggctgcatca attgccagtt ccaaaaggtt tccttcacct gtttcggcac ttttggtaat
      901 tgcctgcagt gctttttcca cttcttcact attgcgttct cggcgtaatt tttccaaacg
      961 ggcaatttgt gtatccctta ctgcggagtt atccacttcc aaaatttcaa tagggatttc
     1021 cgtttcggga ggatatttat tcacgcctac aataatatcg gctccggaat caatctttgc
     1081 ctgccttctg gcagctgctt cttcaatcct cattttagga agccctgttt caatcgcttt
     1141 tgccattcca cccaggtttt ccacttctat aatatgtttc caggcacttt tcattaaagc
     1201 atcggttaaa aattccacat aataggaacc agcccaaggg tctattactc tgcaaattcc
     1261 ggtttcattt tgtaaataga gctgtgtgtt tctggcaatt ctggcagaaa aatcagtcgg
     1321 caaggcaatt gcttcatcca aagaattagt atgcagggat tgcgtatgac ccattgtagc
     1381 ggctaatgct tcaatgcagg ttctggtaat attattatag gggtcttgtt ctgttaaact
     1441 ccagccggag gtttggctat gagttcttaa agccattgat ttcggatttt tagggttaaa
     1501 ctgcttaatt atttttgccc aaagaactct ggcaccacgc aattttgcca cttccataaa
     1561 atagttcatt ccctctgccc aaaagaagga aagacgcgga gctataacat caatatccaa
     1621 tccggctttc aaagctgtgc gcacatattc taaaccgtct gctaaagtat aagccatttc
     1681 caaatcggca gttgctcccg cttcatgcat atgatagccg gaaatactga tactattgaa
     1741 tttaggcata ttttttgccg tgtagctaaa aatatccgct attatgcgca ttgagggttc
     1801 aggaggataa atataagtat tccgcaccat atattctttc aaaatatcat tctgaatagt
     1861 tccattca
//
LOCUS       DAOPFL010000207         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_90603_length_1840_cov_83.001681, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000207 DAOPFL010000000
VERSION     DAOPFL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90603_length_1840_cov_83.001681"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLG69_08020"
     CDS             <1..237
                     /locus_tag="PLG69_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPI43489.1"
                     /translation="LCRYAEGAFYPLNDNFAYLGSGKYNGVFPVSDVWLQFGYRVSEK
                     QTRVFLQPLKPKVDDTLSLEIIAEDYPFEKKEEN"
     gene            296..1099
                     /gene="mutM"
                     /locus_tag="PLG69_08025"
     CDS             296..1099
                     /gene="mutM"
                     /locus_tag="PLG69_08025"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPI43490.1"
                     /translation="MPELPEVQSIINDLEKVLKGKTIKTIECYYSGTVITNCLPEDNP
                     FPIRALAIKRRGKYIIILLERDIALIIHLRMTGKLIYDNFCGEPLKYERARIILENNE
                     VLHFIDIRTFGKIVICSQKNIEHCLPPLGLEPLSDDFTAKALKEKMRGKKTPVKIALM
                     DQKIIAGLGNIYVCEILYRAGINPEKPANKITRKNIVEIIQQTKEVLTEAIDKGGTSI
                     SDYRRIDDKPGSFQNFLQVYQKQFCPLGHKVLRLKQGGRSTFYCPICQK"
     gene            1302..>1840
                     /locus_tag="PLG69_08030"
     CDS             1302..>1840
                     /locus_tag="PLG69_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPI43491.1"
                     /translation="MKAIKLKVGVYWVGVIDWDLRNFHGYITQRGSTYNAYLIIDEKV
                     TLIDNVKSYLFEEMLSRISDIIDPSKIDVLIQNHTEMDHSGGLPELIKYLPNTAIYTN
                     ANGIKALKRHFKQDWNFNEIKSGDSIKIGKRTLTFLTTPMVHWPDNQFTYCPEEKILF
                     SNDGFGQHIASNERFAKELP"
BASE COUNT          625 a          305 c          380 g          530 t
ORIGIN      
        1 ctttgccgct atgcagaggg agcgttttat cccttaaatg ataattttgc atatctgggc
       61 tcgggaaaat ataatggtgt ttttcctgtt tcggatgtct ggctacagtt tggatatagg
      121 gtttcggaaa aacaaacaag ggtcttcctg caacccttaa agccaaaagt ggatgataca
      181 ctatccctgg aaattattgc agaggactat ccttttgaga aaaaggaaga aaattagctc
      241 tcacagatta cacggatttc ccagataaaa gatgaataat aaaaggagag gataaatgcc
      301 ggaattgcct gaagtgcaaa gcattatcaa cgatttggaa aaagtgctga aaggcaaaac
      361 tataaaaaca attgaatgtt actattccgg aactgtgatt acgaattgtt tgccggaaga
      421 taatcccttt ccaattaggg ctttggcaat aaaacgccgc gggaaatata ttattatatt
      481 gctggaaagg gatattgcct taataattca cttgcggatg acaggtaaac tgatttatga
      541 taatttttgc ggtgaaccct taaaatacga aagagccaga ataatcctgg aaaataatga
      601 agttctgcat tttatagata ttcgcacttt cggaaaaata gttatctgca gtcaaaaaaa
      661 catagagcat tgccttccgc ctttgggact ggaaccctta agcgatgact ttaccgctaa
      721 agctttaaaa gaaaaaatgc gcggcaaaaa aactcccgta aagattgctt taatggacca
      781 gaaaatcatt gccggattgg gaaatatcta tgtttgtgaa attctctatc gggctggaat
      841 taatcctgaa aaaccggcaa ataaaattac tcggaagaat attgtggaaa ttattcaaca
      901 gacaaaggaa gtccttacgg aagcaataga taaaggtgga acaagtattt cggattaccg
      961 cagaattgat gataaaccgg gcagttttca aaacttcctg caggtttacc aaaagcagtt
     1021 ctgtcctttg gggcataagg ttttacggct gaaacaaggt ggacgttcta cattctactg
     1081 ccctatatgc caaaaataaa aattacttga cctacttctt tatctcagat attatataat
     1141 tatgttagtt ctatattgaa agttctgtaa gacagattaa caaatatctg gattcctgtt
     1201 tgcacaggaa ggataaagga attttcaaaa gagaaatgat tttatataga tgaacgatga
     1261 ggacatcgtt cttccgcata aaaaagaaga tggatagagg tatgaaagca attaagctga
     1321 aagtaggtgt ttattgggtt ggagttattg actgggattt gcgtaatttt catggttata
     1381 taactcaacg gggtagcaca tataatgcct atctgattat agatgagaag gttaccctta
     1441 tagataatgt gaagagttat ctgtttgaag aaatgctttc ccgaataagc gatataattg
     1501 atccctccaa gattgatgtt ttaattcaga atcatacgga aatggatcat agcggtggct
     1561 tgccggaact gattaaatac cttcccaata cggctatcta cactaatgcc aatggtataa
     1621 aagctctgaa aaggcatttt aagcaggact ggaattttaa cgaaatcaaa agcggagaca
     1681 gcattaaaat cgggaaaaga actttaactt ttttaactac cccgatggtg cactggcctg
     1741 ataatcaatt tacttattgt cccgaagaga aaatcctgtt ttccaacgat ggtttcggtc
     1801 agcatattgc ctctaatgaa cgctttgcca aagaacttcc
//
LOCUS       DAOPFL010000208         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_91463_length_1828_cov_110.695431, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000208 DAOPFL010000000
VERSION     DAOPFL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91463_length_1828_cov_110.695431"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1828
                     /locus_tag="PLG69_08035"
     CDS             <1..>1828
                     /locus_tag="PLG69_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPI43492.1"
                     /translation="GSISFALRGKALVSGNWEGSVQQYGFEQGVRYRLPQILPDGKHL
                     LLLSDEPGYERFEIHPLRKDFEKVSEEVVIIDNPDLGRVTNYVPAPKGSLVAIENHRC
                     ELIVLDLESKEFKNIDRNKFGLISELNWSPDGRWLTYSINRDRNTRHIIIWDSKENQS
                     HIVSKPVLNDTVPVFDPEGKYLYFASVRNLNPLRDSIQFDFIYPNSYKPYLIPLRKDL
                     RSPFIPEPKPFEIKPETPKKEEKIEAKEQKEEVKPVEIDFDGICDRLIEFPVQPGIIG
                     GLGAIKDRLFYVNMLLESSMDEEPKFDLMCYDLNKLEGWTYAKDITYYNISQDGSAIL
                     IRQKDKVRVISAKLEPKAELPADATPGKKSGYIDLNRFSVEIVPIQEWQQMFKEAWRL
                     QTQHYWVEDMAGIDWQKVFDRYYPLVERCACRSDFSDLMWEMQGELGTSHCYEFGGDY
                     RQSPNYRIGKLGIDYRYNARKKGYEIVRIIKGDHWLSENRSPLMNPGMNVSEGWIIKA
                     VNGIPVTKTIPPERLTLNLAGQQVQLTFSSPDGKEEKVVTVPTMKDESQARYRDWVEG
                     NREYVHKVSKGKLGYVHIPDMQKDGLIEFHRYFLAEVDYEGLI"
BASE COUNT          566 a          332 c          418 g          512 t
ORIGIN      
        1 ggttccatct cctttgcctt gcgcggaaaa gcattagtga gtggaaattg ggaaggcagt
       61 gtgcaacaat acggttttga acaaggtgtg cgttatcgtc tgcctcaaat tttgcccgat
      121 ggtaaacatc tacttttgct ttccgatgag cccggttatg agcgttttga aattcaccct
      181 ttgcgtaagg actttgaaaa ggtttccgaa gaagtagtaa ttattgataa tcccgattta
      241 ggcagagtta caaattatgt tccggctccg aagggctcgc tggttgctat tgaaaatcat
      301 cgttgtgagc tgatagttct ggatttggaa agcaaagaat ttaaaaatat agaccgtaac
      361 aaatttggct taattagcga acttaattgg tcaccggatg gacgctggct tacttatagc
      421 ataaatcgtg atagaaacac ccgccatatt attatttggg acagcaaaga aaatcaatcg
      481 catatagtta gtaaacccgt gttaaatgat actgtccctg tttttgatcc ggaaggtaag
      541 tatctctatt ttgcctcggt acgcaattta aaccctttac gggatagcat tcagtttgat
      601 ttcatttatc ccaattccta taagccctat ttgattccac taagaaaaga cctgcgttct
      661 ccttttattc cagaacccaa gccctttgaa ataaaaccgg aaacccctaa gaaagaggaa
      721 aagatagaag cgaaagaaca aaaagaagaa gttaaacccg tagaaattga ttttgacggt
      781 atctgcgacc gcttaataga atttccggtt cagccaggaa ttatcggagg tcttggtgca
      841 atcaaagacc gcttgttcta tgtaaatatg cttctggaaa gcagtatgga cgaagaaccc
      901 aagtttgatc tgatgtgcta tgatttgaac aaactggagg gttggactta tgctaaggac
      961 attacttatt ataatatcag ccaggatggc tctgctattt taatcagaca aaaagataaa
     1021 gtccgcgtta tttctgccaa gcttgaaccc aaagcggaat tgcctgctga tgcaacaccc
     1081 ggcaaaaaga gtggctatat agatttgaac agattttcgg tggaaattgt acctatccag
     1141 gaatggcaac agatgttcaa agaggcctgg cgtttacaga cacaacatta ttgggtggaa
     1201 gatatggcag gaattgattg gcaaaaggtt tttgatcgct actatccttt agttgagcgt
     1261 tgtgcttgca gaagtgattt ttccgatttg atgtgggaaa tgcaaggtga attaggaacc
     1321 agccattgtt acgaatttgg cggggactat cgtcaaagtc ctaattaccg cattggcaaa
     1381 ctgggaattg attatcgcta taatgctcgc aaaaagggtt atgaaattgt gcgcattata
     1441 aaaggtgacc actggctaag cgaaaatcgc tcaccactta tgaaccccgg aatgaatgtt
     1501 tccgagggct ggataattaa agccgtaaac ggaattcctg tcactaaaac gatacctccg
     1561 gaaaggttaa ctctaaattt agccggacaa caggttcagt taacattttc cagtcccgac
     1621 ggcaaagaag agaaagtggt aactgtgcct acaatgaaag atgaatcgca agcccgttat
     1681 cgggattggg tagaaggaaa tagggaatat gtgcataagg tctcaaaagg aaaactgggt
     1741 tatgtccaca ttccggatat gcaaaaagat ggtttaattg aatttcatcg ctattttctg
     1801 gcagaagtgg attatgaagg actgatta
//
LOCUS       DAOPFL010000209         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_93636_length_1799_cov_80.302752, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000209 DAOPFL010000000
VERSION     DAOPFL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93636_length_1799_cov_80.302752"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..693
                     /locus_tag="PLG69_08040"
     CDS             94..693
                     /locus_tag="PLG69_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPI43493.1"
                     /translation="MSAAQNKKKLILDIATQLFTRFGFAKTSLDEIATAAQIAKGTVY
                     YYFPSKEDLFIASVEAKTREYFKMLRQHINSTTGFEEKLTEFLSFPIKLIYENMPILI
                     ECLINIPQSYQEQLIQFRVKSREVMMQILSEIIQLGKQEDLIDERFPEDRLCEIIMDW
                     FMIGEPSLKIVDVPRLMTMIERDSDFIIKMLLYGIVKRG"
     gene            697..>1799
                     /locus_tag="PLG69_08045"
     CDS             697..>1799
                     /locus_tag="PLG69_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPI43494.1"
                     /translation="MRKTLIVLTLLTLLASCVFAIDLETSITKAKQNNKELLMAMEEI
                     KKADESYKQVRGTAYPQLNLQGAYGLSKTYYPDSAIPAKMNISAGLDDTATDNEEYLA
                     GVLNKIVNSMIPESPAEAGSFALQLKMEQIVYSGGKLGSGIKAGNYYRQIQRLNYKVK
                     EQDVVLKTTELFYQCLLAKKLWDVQEEGLDIAKRHLRRVELFNQEGQVSEFDLLQAKL
                     GVAKLEPQVLKAKNDYDLAVSAFRKQIGEEESDIVPEGEFVLPEKMELSLEEATEQGL
                     KQRNEVELIKLGTQLKELQYKVEKVNFLPNVALSADYSLYTAADEYAIENDDFGHKYG
                     FSLGFQIPLFTGLTNTAKRNYARYDYQKAKLQQ"
BASE COUNT          603 a          290 c          408 g          498 t
ORIGIN      
        1 gcttgactat atatactgtt tgaccgtaat gggtcagttg ttcagtttca aagctgtaac
       61 cttcaggaat ataataaaat ataaggaagg agaatgagtg ccgctcagaa caaaaaaaag
      121 ttgatattgg atatagctac ccagctattt acccgtttcg gcttcgccaa gacctcgttg
      181 gatgaaattg caactgcagc tcagatagct aagggaacgg tatattatta ctttcctagc
      241 aaggaagacc tctttattgc ttcggtggaa gccaaaacga gagagtattt caaaatgttg
      301 aggcagcata tcaattccac gactggtttt gaagagaagc tgacggaatt tttaagcttt
      361 cccatcaaac tgatttacga aaatatgccc atcctgattg agtgtttaat taatataccg
      421 caaagttatc aggaacagtt gattcagttc cgagttaaaa gcagggaagt gatgatgcaa
      481 attctttctg aaataatcca gcttggaaaa caggaggatt taattgatga gcgctttccg
      541 gaagaccgtc tttgcgagat aattatggat tggtttatga taggtgaacc gagtttgaaa
      601 atagtagatg ttccccgctt aatgacaatg attgagcggg atagtgattt tattattaag
      661 atgctacttt atggaattgt taaacgagga taaaaaatga gaaagacatt aattgtatta
      721 acactgctaa cattattagc aagctgtgta tttgccattg acctggaaac tagcatcacc
      781 aaagctaaac agaataacaa ggaactgtta atggcaatgg aagaaattaa gaaagcggat
      841 gagtcataca aacaggttcg tggcactgct tatccacaat tgaatttaca gggtgcttac
      901 ggtttaagta agacctatta tcctgattca gctatacctg ctaagatgaa catatctgcc
      961 ggcttagatg atacagcaac agataatgaa gaatatttag cgggtgtact gaataaaata
     1021 gttaattcaa tgattccgga atcgccggca gaagcgggtt cttttgcttt acagttaaaa
     1081 atggaacaga ttgtttattc cggaggcaaa ctgggaagcg gaattaaagc gggaaattat
     1141 taccgccaaa ttcagcgact gaattataaa gtaaaagaac aggatgtggt tttgaaaacc
     1201 acggaactct tttatcagtg cttactggca aaaaagctgt gggatgtgca agaagagggc
     1261 ttagatattg ccaaaaggca tttgcgcaga gtggaactgt tcaatcaaga aggtcaggtt
     1321 tcggaatttg acttgttaca agccaaatta ggtgtagcta aactggagcc ccaggttttg
     1381 aaagcaaaaa atgattatga tttggctgtt tcagcttttc gtaaacagat tggagaagaa
     1441 gagagtgaca tagttcccga aggtgaattc gtgttgccgg aaaaaatgga gctctctctg
     1501 gaagaggcaa ctgaacaggg tttgaaacaa agaaatgaag tagaactgat taaattgggt
     1561 actcagttaa aagaactgca atataaggtg gaaaaagtga atttcctgcc caatgttgcc
     1621 ttgagcgcag attattcact ttatacagcg gcagatgaat atgctattga gaatgatgat
     1681 ttcgggcata aatacggctt cagtttgggc tttcagattc ctttgtttac cggcttaacc
     1741 aatacagcca aaagaaatta tgcccgctat gattatcaaa aagccaaact gcaacaaag
//
LOCUS       DAOPFL010000210         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_93702_length_1798_cov_84.751578, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000210 DAOPFL010000000
VERSION     DAOPFL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93702_length_1798_cov_84.751578"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..532
                     /locus_tag="PLG69_08050"
     CDS             176..532
                     /locus_tag="PLG69_08050"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPI43495.1"
                     /translation="MLRQILLAKLHRATVTECDLNYNGSIKIDEELLEKSGMREFEQV
                     DVFDITNGNRFSTYIIVGERGSGVIGINGAAARLVQVGDKVIVMNYGIMDETEMENHK
                     PCIILLNDNNKIESIL"
     gene            532..1371
                     /gene="panC"
                     /locus_tag="PLG69_08055"
     CDS             532..1371
                     /gene="panC"
                     /locus_tag="PLG69_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPI43496.1"
                     /translation="MQIIDNIQAMQEIGNNWSAGKKIGFVPTMGYFHQGHLSLVGKAN
                     KQCEITVVSIFVNPSQFGPNEDFESYPRDMQRDLELLSKYKVDYVFSPNPEQMYPQDY
                     RTWVEVEGLSSILCGASRPGHFRGVATVILKLINIIKPNFMFMGEKDFQQVTVLQTML
                     KDLNCETQIVSCPIIREEDGLAMSSRNIYLNPEQRKQALCLYRAIQQAQAKYKQGLRD
                     TNLLKKQAADLIINSGGKIDYISFVEPKMLQEVAIADDNTRIMLAVFIGKTRLIDNAP
                     LKV"
BASE COUNT          602 a          293 c          365 g          538 t
ORIGIN      
        1 gtcaaccgct ttttttcaac tacctgaaaa atccctctcc accttaacct aattatatta
       61 taatgcagtt ccttagaaaa gcaaattatt tttttaccgc gttttcccaa ggggaatttt
      121 attgacaaaa gggacagctt ttgattatga ggacaaaaaa agcaggagaa aatgaatgtt
      181 acgacaaatc cttttagcaa aattgcatcg tgctaccgta acagaatgcg acctgaatta
      241 taacggcagc atcaaaatag atgaagaact actggagaaa agtggaatgc gggaatttga
      301 gcaggttgat gtttttgaca tcactaatgg gaatcgtttt tctacttata tcatagttgg
      361 agaacgcgga agtggagtta tcggaattaa tggagcggca gcgcgtttag ttcaggtcgg
      421 ggacaaagtg atcgttatga attacgggat tatggatgaa acagaaatgg aaaatcacaa
      481 accctgtata atattgttaa acgacaataa taaaattgag agcattctct gatgcagata
      541 attgataata ttcaagcaat gcaagaaatc ggtaataatt ggagtgctgg caaaaaaatc
      601 ggttttgttc ctacaatggg ttattttcat caagggcatc tttcattggt agggaaagcg
      661 aataagcaat gtgaaataac agttgtttcc atttttgtaa atccttcgca gtttggacct
      721 aacgaggatt ttgagagtta cccgcgagat atgcagcgtg atttggaact gcttagtaaa
      781 tataaagttg attatgtttt cagtccgaat cctgagcaga tgtatcctca ggattataga
      841 acctgggtag aggtagaagg tcttagttca attctttgtg gagcaagtcg tccagggcat
      901 tttagggggg tagctacagt cattttaaag ctgattaata tcatcaaacc aaactttatg
      961 tttatgggag aaaaagattt ccagcaggtt acagttttgc aaacaatgct gaaagatctg
     1021 aactgtgaaa cccaaatagt ttcctgtccc attatcagag aagaagatgg attggctatg
     1081 agcagccgca atatatatct caatccggaa caacgcaaac aagcattgtg tctgtatcgg
     1141 gcaattcaac aagctcaagc taagtataaa cagggtttac gggacactaa tttgctaaaa
     1201 aaacaggcgg cagatttaat cataaattcc ggtggcaaaa ttgactatat cagctttgta
     1261 gagccaaaga tgctgcagga agttgctata gctgatgata atacgcgcat tatgttagct
     1321 gtttttatcg gtaaaacccg gttaatagat aatgcaccat taaaagttta acatttatcc
     1381 cttcagttag ttaggtagtc ctgccctgat gctttacctt ttatatattt ttattttcct
     1441 cttataatca agtaacaacc aagtaacaac tccgtaacgg aactacggca gtgttacgga
     1501 agtgttacgg agtcgttact taatcatcta tacaacaagc aattatgcct tacagctcag
     1561 agaagacaaa atatgatgct aattgcatta ttaacagtga gtaatatgat atttgagatt
     1621 gccgtttcca cttttttcgg gtgatggata taacttatga aaaattactg taataccaat
     1681 atacagatac caaaaaatat tattttacct gacgaaatct ggtcgttttt tacaaaatat
     1741 aaagtaatat ctaaacgaga aacattggat cattgcagat gtatgtgcta atacatag
//
LOCUS       DAOPFL010000211         1756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_96998_length_1756_cov_87.897119, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000211 DAOPFL010000000
VERSION     DAOPFL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96998_length_1756_cov_87.897119"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1207
                     /locus_tag="PLG69_08060"
     CDS             371..1207
                     /locus_tag="PLG69_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPI43497.1"
                     /translation="MNGIVFFKTRELGLIRNFYASLLALPVWLEQDSCVIFKAGNQLL
                     GFCAGESSEICGTITFFFDGREQVDSAYKKLKSIAKTAPQENPAFKIYHFWATDPEGR
                     TLEFQCFEQQLEPYLTLDEGLKTRRSIRHYTSEPVSDELLNKVFELCRYSPTACNLQG
                     YYYVVIKNREILEKIVALRGPSGKPILASPFAIAVASQGDISHRKIQDACIAAYHLLL
                     SAKAYGLGTCWVTDMDKDEIKDLLGIPHQDYIACLTPIGYPAEHFPVPERHQVSDFVR
                     YL"
     gene            1204..>1756
                     /locus_tag="PLG69_08065"
     CDS             1204..>1756
                     /locus_tag="PLG69_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPI43498.1"
                     /translation="MKPILFLTRRIPEPAIKRLEEKFTLKINPYNRALTHQELIEGVK
                     DAEALICLLTDNIDKEVISAAPKLKVISSYAVGYNNIEVEYATQLGIAVCNTPGVLTE
                     TTADLTWALILATCRRISESERFLRKGNFKGWEPMLMLGLDVYGKTLGIIGMGRIGQA
                     VAKRATGFAMRIIYYNDVPVSNTL"
BASE COUNT          507 a          333 c          386 g          530 t
ORIGIN      
        1 ttttttacga ggggcttaca ccaccatctc tatgattgtg tcggggctgt cccgatacaa
       61 cgataacgat gggtgtgtaa accactcgga taaaaaaggt aaagattttc ccattattcc
      121 aaatccaatc agaaaatatt tctaagcgtc ccttgcttca aacccaacat caacccaacc
      181 tttaggatgg gtagatgctg agtggaggat ggggtagatg atgggttgta agtaagtaag
      241 taagttccta ttgctgtgca gggttaaact tgtagctata aactcaaata atggtgtcca
      301 aatcaatcgg atttgcctgt taacctgaaa cagattaaaa tatattataa ataggaaaaa
      361 aggagatatg atgaacggaa tagtattctt caaaacaagg gaattgggtc tgataaggaa
      421 tttttacgct tctctgctgg cacttcctgt ctggttagaa caggattcct gtgtaatttt
      481 taaagccggt aatcaattgc ttggtttctg cgcaggcgaa agtagtgaaa tttgcggcac
      541 aattaccttc ttttttgacg gtagagaaca ggttgatagc gcttataaaa agctgaaaag
      601 catagcgaaa acagcgccgc aggaaaatcc agcatttaaa atttatcatt tttgggcaac
      661 tgatcctgaa ggcaggactt tggaatttca gtgttttgaa cagcaattgg agccctatct
      721 tactttggat gagggcttaa aaacccgccg cagtattcgc cattacactt cggaaccggt
      781 ttcggatgag cttttaaaca aggtttttga actttgtcgc tattctccaa ctgcctgcaa
      841 tctgcaaggt tattactatg tagtgattaa gaatagggaa attttagaaa agattgttgc
      901 cctgcgtggt ccttccggta agccgatttt agcttctcct tttgctattg ccgttgcctc
      961 tcaaggtgat atctctcatc ggaaaattca ggatgcctgt attgctgctt accatttatt
     1021 gctttccgca aaagcttatg gcttgggaac ttgctgggtt accgatatgg ataaagatga
     1081 aattaaagac cttcttggca ttccccatca ggattatatt gcctgcttaa ctccgattgg
     1141 ctacccggca gaacattttc ctgttccgga aaggcaccag gttagtgatt ttgtgaggta
     1201 tctatgaaac cgatattatt tttaacccgc cgtattccag aacccgcaat aaagcgtttg
     1261 gaggaaaaat ttaccctgaa aatcaatcct tataaccgtg ctttaactca tcaggaactg
     1321 attgaaggag tgaaagatgc cgaggcactt atttgtttat taaccgataa tatagataaa
     1381 gaagttatct ccgcggctcc aaaattgaaa gttatttcct cttacgcagt tggctacaac
     1441 aatattgaag tggaatatgc cactcaactt gggattgctg tctgtaatac accgggtgtt
     1501 ttaacagaaa ccactgccga tcttacttgg gctcttattt tggcaacctg cagaagaatt
     1561 tcggaaagtg agcgtttctt gcgcaaggga aactttaaag gttgggaacc aatgctgatg
     1621 ttaggacttg atgtttacgg caaaacactc gggataatag gaatgggaag aattggtcag
     1681 gcagttgcca aacgcgctac tggttttgca atgcggatta tctattataa tgatgttccc
     1741 gtttccaata ctttac
//
LOCUS       DAOPFL010000212         1724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_99754_length_1724_cov_66.040743, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000212 DAOPFL010000000
VERSION     DAOPFL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99754_length_1724_cov_66.040743"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLG69_08070"
     CDS             complement(<1..185)
                     /locus_tag="PLG69_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPI43499.1"
                     /translation="MPISKRKLLLITDLYPNKFNPVNGVFVQQQAIELSRHYQVQVVA
                     ACDKNPYLVYEHHQDGFP"
     gene            complement(347..>1724)
                     /locus_tag="PLG69_08075"
     CDS             complement(347..>1724)
                     /locus_tag="PLG69_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPI43500.1"
                     /translation="TLIDIIGVYQQDPGTANWPVAGTPSATAEHTLIRKPTVIQGNTN
                     FLAGAGTNADDSEWIVFPQNYFADLGQHTFNPGAPEQAATPTFNPPAGVYANPISVTI
                     SSTTPGATIRYTTDGTEPNETSTLYSAPIALATTTTLKAKAYATGFSPSYTATANYIF
                     PVQVSNIAALRASAADGTTIYQLANEVILTMQQNWRHQKFIQDATAAILIDDYNNIMN
                     TPYNIGDGITGITGTLNRYTTGMLQFWPVINPGPATSTGNVIIPQLVTIAEITNNAEL
                     YQSRLMRLDNVHYASPSGNYAAQTSYDLLDATGTIVMRTQFSDADYIGTPMHQGNFNV
                     YVIVTQYNATTQVTPRMLSDFNPVANDDNVLTPMQIELIGNYPNPFNPETTIRFKMEK
                     SAPAEVIIYNDKGQIVKTVNTIATQGINSVVWDGKDNNGYPVASGVYLFRLKSGSYSS
                     TKKMVLMK"
BASE COUNT          508 a          320 c          362 g          534 t
ORIGIN      
        1 ggaaagccat cctgatgatg ttcatataca agataaggat ttttatcaca agcagcaaca
       61 acttgaacct gataatgcct ggaaagttca attgcttgct gctgaacaaa cacaccattt
      121 acaggattga atttattggg atataaatcc gttatcaaca gaagttttcg tttggatatt
      181 ggcattgctc tctaaattgt aaaacagcat actgacatct ttgctttctt actttataaa
      241 tagtaggttg tcagtatgct gtttactgtg tagtcaaaag tcgttttggt gtagtcggag
      301 ctcgctgaat ctcccttttt tcttgaggag gaacagcatc ctccggctat ttcatcaaaa
      361 ccattttttt ggtgctactg tagcttcctg atttcagacg gaacagataa actccactgg
      421 caacaggata tccattatta tcttttccat cccaaacaac actgtttatt ccctgtgtag
      481 caatagtgtt aactgtttta actatctgac ctttatcgtt gtaaataatc acttccgccg
      541 gagctgattt ttccattttg aaacgaatag ttgtttcggg attgaaagga ttaggatagt
      601 tgcctatcag ttcaatttgc ataggtgtta acacattatc atcattggca acgggattga
      661 agtcacttag cattctggga gtaacctgtg ttgtagcatt atactgagta actataacat
      721 acacattaaa atttccctga tgcataggag ttccaatata atccgcatca gagaattgag
      781 ttcgcattac aatagtgccc gtagcatcca ataaatcata actggtttga gcagcataat
      841 ttccgctggg gctagcatag tgaacattat ccagacgcat taaacgtgat tgatagagtt
      901 ctgcattgtt agttatctct gctatagtta ccaattgtgg aataattaca ttacctgtag
      961 aggttgcagg accgggattt attactggcc aaaattgtag cataccggta gtgtagcgat
     1021 ttagagttcc tgtaatgcct gtaattccat ctccgatatt ataaggagtg ttcataatat
     1081 tattataatc atcaatcaaa atagcagctg ttgcatcctg aatgaacttt tggtgacgcc
     1141 agttctgttg catcgtaagt attacttcat tggcaagctg ataaatagtt gttccatcag
     1201 cggcagaagc acgtaaagct gcaatattgg aaacttgcac cgggaagata taattagcag
     1261 ttgctgtgta agaaggacta aaaccggtag cataggcctt ggctttcaga gttgtagtag
     1321 ttgccaaagc tataggagca gaataaagag tggatgtttc attcggttct gtgccatctg
     1381 tagtataacg aattgtagct ccaggcgttg tagaagaaat tgttacactg ataggatttg
     1441 catagacccc tgcaggagga ttaaaagtag gagttgctgc ttgttcggga gctccgggat
     1501 taaaagtatg ctgacccaaa tcggcaaaat aattctgcgg aaaaactatc cactccgaat
     1561 cgtcagcatt agttccagct ccagcaagaa aattggtatt gccctgaata acagttggct
     1621 tccgaattaa agtatgttcc gcagtagcag aaggagttcc ggcaacaggc caattggcag
     1681 ttcctgggtc ttgttgataa acaccgataa tatcaataag agta
//
LOCUS       DAOPFL010000213         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_104407_length_1672_cov_81.419295, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000213 DAOPFL010000000
VERSION     DAOPFL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104407_length_1672_cov_81.419295"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1672)
                     /locus_tag="PLG69_08080"
     CDS             complement(<1..>1672)
                     /locus_tag="PLG69_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI43501.1"
                     /translation="KTDIYSKNEFYSGELFSVEEIGFLRGVRIFRIDYEPMRYNPVSG
                     ELKINTELNVQVKFINADFSATQDLLARTASYEFDSLYGKILLNWQKNERLNVNRYPT
                     KMVILCPPNYVSTMQTFVNWKTQQGYKVIVTTVGTGGTIANNTTAIKNYMQTLWNNAT
                     ASDPAPTYLLIVGDTSTSGDNIIANTGATSSSHITDLTYVRLNGTDFLPELYFGRFSV
                     ASATELTNVINKTITFEKTTMPDLSYLGKTVLIAGVDSNFGPTHGNGAINYGTTQYFN
                     SAHNITSNTYLYPASGSSDAQIIANASEGRGYINYTAHGSETSWYDPAFSISDINGLN
                     NTNKYSVVVGNCCLTNAFNTGVCFGEAFLRANNKGAVSYIGGTNSTYWDEDYWWAVGS
                     KGTANGSAPAYDASKLGAYDAMFHTHSEAFTNWATTVGETVMMGNSAVEQSASSRKNY
                     YWEIYSIMGDPSLMPYYGVPTTNTATFPASIIIGATSITVTATPYSRVALSKDGVLYG
                     SDIVPASGTLTLTINPITTAGTATLVITAQNKITRIENITVAAGDTPYI"
BASE COUNT          510 a          325 c          321 g          516 t
ORIGIN      
        1 taatataagg ggtgtcacca gcagctacag taatattttc tattctggta atcttgtttt
       61 gagcggttat taccagagta gctgttcctg cagttgtaat aggatttatg gttaaagtta
      121 aagttccgct ggctggaact atatctgagc catacagaac tccgtctttg gaaagagcaa
      181 cccgggaata gggtgtggca gttacagtta tagaggtagc accaattata atagaggcag
      241 gaaaagtggc agtattggtt gtcggaacac cataataggg catcaaagaa ggatcaccca
      301 ttatggagta aatttcccaa taataattct ttcgggaact tgcgctttgt tcaactgcgc
      361 tattacccat cataactgtt tcgccaactg tagttgccca attggtaaat gcctcgctat
      421 gagtatgaaa catagcatcg taagcaccta attttgaagc atcataagca ggagcacttc
      481 cattggctgt tccttttgag cccactgccc accagtagtc ctcatcccag taagtagaat
      541 ttgttccgcc aatataagaa acggcacctt tgttattagc tctaagaaat gcttctccga
      601 aacaaacacc tgtattaaaa gcattggtta agcaacaatt acctactaca acggaatatt
      661 tgttggtgtt gttaagtccg tttatatcac taattgaaaa ggcaggatca taccagctgg
      721 tttcacttcc atgagctgta taattaatat aaccccttcc ttctgaggca tttgctataa
      781 tttgagcatc acttgaaccg gaggcaggat aaagataggt attggaagtg atattatgag
      841 cgctattaaa atattgggta gtaccataat ttatagctcc attgccatgt gtaggtccaa
      901 aatttgaatc gacaccggca attaaaacgg ttttgcctaa ataactcaaa tccggcatag
      961 tggtcttttc aaaagttatg gttttattga ttacattagt caattccgta gcactggcaa
     1021 cggaaaaacg accaaagtaa agttccggca gaaaatcagt tccgtttaac ctcacataag
     1081 taagatccgt aatgtgagaa ctactcgtgg ctcctgtatt ggcaataata ttatctccgc
     1141 tggtagaagt atcacctaca attaataaat aggtaggagc cggatcactt gctgtggcat
     1201 tattccataa tgtctgcata taatttttaa tagcagtggt attattagct atagttccac
     1261 cagttccaac cgtagtaaca atcactttat agccctgttg tgttttccag tttacaaagg
     1321 tctgcattgt actaacataa ttaggaggac ataaaatcac catttttgtc ggatagcgat
     1381 taacattcag gcgttcattt ttctgccagt tcagcaaaat tttgccgtat aaggaatcaa
     1441 attcataaga agcagtgcgg gctaataaat cttgagtagc agaaaaatct gcatttatga
     1501 attttacctg cacattcaat tccgtgttta ttttcagttc tccgcttaca ggattataac
     1561 gcattggttc ataatcaata cggaagattc ttactccacg cagaaaacca atttcttcca
     1621 cggagaaaag ttctccgcta taaaattcgt ttttactgta aatatccgtt tt
//
LOCUS       DAOPFL010000214         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_104966_length_1666_cov_105.376164, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000214 DAOPFL010000000
VERSION     DAOPFL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104966_length_1666_cov_105.376164"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1605
                     /locus_tag="PLG69_08085"
     CDS             163..1605
                     /locus_tag="PLG69_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPI43502.1"
                     /translation="MKYLDLAMNAAEKFGAEYADIRIQKTTDQVIYLQNLSLKHTSNN
                     VLQGYGIRVFKDGAWGFAHSNVFNEEAVLATVKKAVEIATLSAEVNKTQKLRLAPERS
                     YIATYRTPVKIDPFTVPVSEKIDLMMEVARTMLAYEGIMKTVFLMILHKDEKLFASTL
                     GTRLDLTTQFINPMITAVAVTESDSQSRTFDEGGRAVGWEWILELDLREKAKQIAEEA
                     IIKVKADTLGAEERRSLILDPNHLGLTMHESVGHATELDRVLGWEADYAGVSFATPEK
                     LNNYHYGSELINFVGDNTLEGGLATLGFDDDGVPGQKWYIIKDGILKEYGSTRDTALE
                     IGLNASRGCNRATYYFNQPINRIPNLYLLPGTKPLTPGELIADTEDGVYIQGRGSYSI
                     DHHRINFQFGGDFFWEIKNGKLIRPLKKVIYKSYNPEFWNSCDAICDERFWRPFGVVN
                     CGKGQPPQTGRMTHGASTARFRNIRVGGSQ"
BASE COUNT          501 a          290 c          385 g          490 t
ORIGIN      
        1 atatggtctt tggataaaag aggcaatccc aatcgccgta gttttgccca ggcagtttta
       61 atttttgtgg ctgttatgtt agtcctttcc agcttttatt tcgggcagat gactggttgg
      121 ctttttcaaa taatggcttt aatgtagaaa taaggagaaa gtatgaaata tttagatttg
      181 gcaatgaatg cggcggaaaa attcggtgct gaatatgccg atatccgcat tcaaaaaaca
      241 actgatcagg taatttatct gcaaaacctt tccctgaaac ataccagtaa taatgttctg
      301 cagggttatg gcattcgggt tttcaaagat ggtgcttggg gttttgcgca tagcaatgtt
      361 tttaatgaag aagctgtttt agctactgta aaaaaggcag ttgagatagc aactctttct
      421 gcggaagtga ataagacaca gaaattgcgt ttagccccgg aacgcagtta catagctact
      481 tacagaactc ccgtaaaaat tgatcctttc actgttcccg tatctgaaaa gatagacctg
      541 atgatggaag ttgcccgcac aatgcttgct tatgaaggaa ttatgaaaac tgtgttttta
      601 atgattctgc ataaggatga aaaactcttt gccagcactt taggaacgcg tttagacctt
      661 actacgcagt ttatcaatcc tatgattaca gctgtagcgg ttacggaaag tgacagccaa
      721 agccgaactt ttgatgaagg tggaagagct gtcggttggg aatggatttt ggaactggat
      781 ttaagggaaa aagctaaaca aattgccgaa gaagcaatta tcaaagtgaa agcggatacc
      841 cttggtgctg aagaacgcag gtctttaatt cttgatccta atcatctggg tttaacaatg
      901 catgaaagcg ttggtcatgc taccgaactg gatagagttt tgggttggga agccgattat
      961 gctggtgttt cttttgccac accggaaaaa ttgaacaact atcattacgg tagtgaactg
     1021 attaactttg tgggagataa cactttggaa ggaggtcttg ctactctcgg ttttgatgat
     1081 gatggggttc ccggtcaaaa atggtatatt attaaagacg gtatattaaa agaatatggc
     1141 agcacgcgtg atactgcttt ggaaattggc ttgaatgctt cccgcggatg taatcgtgca
     1201 acatattatt ttaatcagcc gattaaccgc attcccaatt tatatttact gccaggaaca
     1261 aagcccttaa ctccgggtga acttattgcc gatactgaag acggagttta tattcaaggc
     1321 agaggcagtt attctattga tcatcatcgc ataaatttcc agtttggagg cgatttcttc
     1381 tgggaaataa aaaacggcaa actgattcgt cccttgaaaa aagttattta caaatcttac
     1441 aatccggaat tctggaatag ttgtgatgct atttgtgatg agcgtttttg gcgtcccttc
     1501 ggggttgtaa attgtggcaa aggtcagccg ccacaaacag gaagaatgac tcatggtgcc
     1561 tcaacggcaa gatttcgtaa tatccgggta ggaggttcac aatgaatagc gaaaaaattg
     1621 tgcaatacat ccagaaacac tgcatagcag atgactggac actaaa
//
LOCUS       DAOPFL010000215         1638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_107625_length_1638_cov_86.019583, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000215 DAOPFL010000000
VERSION     DAOPFL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107625_length_1638_cov_86.019583"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLG69_08090"
     CDS             <1..617
                     /locus_tag="PLG69_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43503.1"
                     /translation="MAEAMLEHSFSRNQKIIAVALWPQGVQMADQAFTVALKKYPEKK
                     YGVDYVNLGYKVAGIVAIQAMGKSMQEVFPSDQSGTKYENIPMLKKVKTLKDVAYICS
                     LSSGTPGIKEWVMAARDPLGTPVTGGTTAVSAPGLLPYVNSQKQLYGLLGGLKAASEY
                     ETLINKIGSATIKMDAQSIAHLLILVFIIIGNIKAFYSRKRGAQ"
     gene            614..1252
                     /locus_tag="PLG69_08095"
     CDS             614..1252
                     /locus_tag="PLG69_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43504.1"
                     /translation="MSGFMTTFGLWLSVFFTLSIFSFLYKDNPFYKLSEQIFVGLSAA
                     YWLVYLYYSVMVPNLFNKLFTDFKGNLILLIPTALGLMMLLRLIPKTQWLSRYPIAIM
                     IGTSAGINMVRYAKTDLLGQISATMINPFASDNVITVIGNILLIVGTICGVYFFYFSK
                     KQEGIRAVPSKLGIWFLMISFGASFGYTVMARISLLIGRLEFLLKDWLHIIK"
     gene            1262..>1638
                     /locus_tag="PLG69_08100"
     CDS             1262..>1638
                     /locus_tag="PLG69_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPI43505.1"
                     /translation="MTKNKDSAMLFNDVFNNVLPFRNLIMRLSKIAKVIHQQGWAEAN
                     AGNISIRISNLLLPFIRTNSWENSYPYQDWFLVSRTGSRFRDFSDEPENCLMIIGIDK
                     EEHYFPDTAKPTSEWLCHRKLQE"
BASE COUNT          473 a          324 c          325 g          516 t
ORIGIN      
        1 caatggcaga agcaatgctg gaacattctt tttcccgcaa tcaaaaaatt attgccgtgg
       61 ctctttggcc tcaaggtgtg caaatggcag accaggcatt tacagtagcc cttaaaaaat
      121 atcctgagaa aaaatatggt gtggattatg ttaatttggg ctacaaagtt gcaggcattg
      181 ttgctattca agcaatggga aaatcaatgc aagaggtttt tccctccgat caaagcggga
      241 caaaatatga aaatattcca atgctgaaaa aggttaaaac cctgaaagat gttgcctata
      301 tttgttccct ttccagtgga actccaggaa ttaaagaatg ggttatggct gcccgcgatc
      361 ctttaggaac tcctgttaca ggcggaacta cagcagttag cgctcctggt ttattacctt
      421 atgtaaatag ccagaaacaa ctttatggct tacttggcgg acttaaagct gcctcagaat
      481 atgaaaccct gatcaataaa attggcagcg ctactatcaa aatggatgca cagtccattg
      541 ctcatctgct gattttggtt ttcatcatta tcggaaatat aaaagctttc tatagcagaa
      601 aaaggggtgc acaatgagcg gatttatgac taccttcgga ctttggctat cagtattctt
      661 cactttaagc atcttcagct ttctctataa ggataatcct ttctataaac tctccgaaca
      721 aattttcgta ggactttccg ctgcctactg gttggtctat ttatattata gcgttatggt
      781 tcccaatctg ttcaataaac tctttaccga ttttaaggga aacctgattt tgcttattcc
      841 taccgctttg ggtttgatga tgctgctgcg cttaattcca aaaacgcaat ggcttagccg
      901 ttatcccatt gcaattatga ttggcacttc tgccggcatt aatatggtac gctatgctaa
      961 gacagacctt ttgggacaaa tcagcgctac aatgatcaat ccctttgctt ccgataatgt
     1021 tataactgta atcggtaata tacttttgat tgtaggaacc atctgcggtg tttatttctt
     1081 ctatttcagc aaaaaacagg aaggcatcag agctgttcct tccaaactgg gaatctggtt
     1141 tttgatgatt agttttggcg cttcttttgg ctatacggtt atggctcgta tctctttatt
     1201 gataggacgg ctggaatttt tgctgaagga ttggctgcac atcattaagt gaagcagtga
     1261 aatgaccaaa aataaagatt cagcaatgct atttaatgat gtttttaaca atgttctacc
     1321 cttcagaaat ctgataatgc gtctaagcaa aatcgctaaa gttatccatc aacaaggttg
     1381 ggctgaagct aatgccggaa atatttctat ccgtatatcc aatttgctgc taccctttat
     1441 caggacaaac agttgggaaa attcctatcc atatcaggat tggttccttg tttccagaac
     1501 tggcagccgt tttcgggatt ttagtgatga accggagaac tgtttaatga taatcggtat
     1561 tgataaagaa gaacattact ttcctgatac tgccaaacct acttccgaat ggctctgtca
     1621 ccgtaaactg caagaaat
//
LOCUS       DAOPFL010000216         1602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_111251_length_1602_cov_96.253394, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000216 DAOPFL010000000
VERSION     DAOPFL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111251_length_1602_cov_96.253394"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /gene="gltX"
                     /locus_tag="PLG69_08105"
     CDS             <1..1424
                     /gene="gltX"
                     /locus_tag="PLG69_08105"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPI43506.1"
                     /translation="FAPSPTGFLHIGGLRTALYNYLFARKEQGTFILRIEDTDQSRIV
                     PGAIENLITSLHNLGLDFDEGPGKEGNFGPYIQSQRLNLYQEQAQYLLEKGFAYPCFC
                     SSETLTNMRKEQQEKGEFVKYDRRCLALSQSEIRQKMANGEKYVIRLKMPDKHKFRFY
                     DLIRGEVEMDSSQSDDQVLLKSDGFPTYHLASVVDDHFMQISHIIRGEEWLSSTPKHI
                     YLYECLGWEIPQWVHLPLILNPDRTKLSKRMNDVSVDSYLLKGYLKEAIINFVALLGW
                     HSADERELFTLDELCEVFSLERVNKANAIFDLAKLDWMNGQYLRLLPLEEIALRCKQL
                     FLEKGFDISDTKKYHLSIAVARERCTLLNEIVEYCKMFYQPETLTEEEEKILSTPEAQ
                     KVLAWFVQKLPTLTNWEVDTIQEVINDGISELGIKGKAFYLPLRLALIGKIHGPELHS
                     TIAIIGLEESVKRLKRHLLKNEE"
BASE COUNT          502 a          289 c          300 g          511 t
ORIGIN      
        1 gttttgctcc cagtccaacc ggttttttgc atattggagg tttgaggact gcactctaca
       61 actatctttt tgcccgtaaa gaacagggaa catttattct gcgtattgaa gatacagacc
      121 aaagcagaat agttcccgga gccatagaaa atcttattac ttccttgcat aatttagggt
      181 tggattttga tgagggaccc ggtaaagagg gaaatttcgg accctacatt cagtcccaac
      241 gcttaaattt ataccaagaa caagcacagt acctattaga aaagggtttt gcctacccct
      301 gtttttgctc ttctgaaaca ctaacaaata tgcgcaaaga acaacaggaa aaaggtgaat
      361 ttgttaaata tgaccgtcgt tgtttagcgt taagtcaatc cgagataagg caaaagatgg
      421 caaatggaga aaaatatgta atccgcttaa aaatgcctga taagcacaaa tttcgttttt
      481 atgacctgat tcggggagaa gtagaaatgg attccagtca atccgatgac caggttttac
      541 taaaaagtga cggttttcct acttatcatc ttgcctcagt tgtagatgac cattttatgc
      601 aaatttctca tataatccga ggtgaagaat ggctttccag cactcccaaa catatctatc
      661 tttatgaatg cctaggttgg gaaattccac aatgggtgca tttaccttta atacttaatc
      721 ctgatcgcac aaagttaagc aaaaggatga atgatgtttc cgtagatagc tatttattaa
      781 agggatactt aaaagaagcc ataatcaatt ttgttgcttt gctgggttgg cattctgccg
      841 atgaacgcga actttttacc cttgatgaac tttgtgaggt cttttctttg gaacgggtta
      901 ataaggcaaa tgctattttt gatttagcta aactggattg gatgaatgga cagtatttgc
      961 gtcttctgcc tttggaagaa attgctttgc gttgtaaaca acttttttta gaaaagggtt
     1021 ttgatatcag cgacacaaaa aaataccatc tatccatagc agttgccagg gaacgctgca
     1081 ctttgcttaa tgaaattgta gaatattgca agatgtttta tcaaccggag acattaacag
     1141 aggaagaaga aaaaatactc tcaactcctg aagcacagaa ggttcttgcc tggtttgtcc
     1201 aaaaattacc tacccttact aattgggaag tagatacaat tcaagaagtt atcaatgatg
     1261 gtatctccga attgggaatt aagggtaagg cattttatct gcctttgcgt ttggctttaa
     1321 ttggtaaaat acacggaccg gaattacatt ctacaattgc tattattggt ttggaagaat
     1381 ctgttaagcg attaaaacgc catctgttaa aaaatgaaga atgaaaaaag tcacgagtcc
     1441 tcatcgctta taaaaattat taccaacctc aaaattattt agcaataagg actcgtgact
     1501 tattaatcct aattctcttt tctcttagta aaattacctc tctttttctc tttccctctt
     1561 tgtaaaatca aatctctttt tctctttctc tctttgtaaa at
//
LOCUS       DAOPFL010000217         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_113113_length_1584_cov_111.882930, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000217 DAOPFL010000000
VERSION     DAOPFL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113113_length_1584_cov_111.882930"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..78
                     /locus_tag="PLG69_08110"
     CDS             <1..78
                     /locus_tag="PLG69_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPI43507.1"
                     /translation="ERNYFMSAEEAMEYGIIDEVISVRK"
     gene            170..1417
                     /gene="clpX"
                     /locus_tag="PLG69_08115"
     CDS             170..1417
                     /gene="clpX"
                     /locus_tag="PLG69_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPI43508.1"
                     /translation="MDKYRTLTCSFCGRGEHEVKNLIRGIAGNICDECIVMCHNIIDS
                     SKEDIKQEDFEAPIPSRIKAYLDQYVIGQDSAKEIISVAVYNHYKRIFKQKYEDDVDL
                     EKSNILMIGPTGSGKTLIAQTLARFLQVPFAISDATTLTEAGYVGEDVENILVRLLQN
                     ANYDVAKAERGIVYIDEIDKISRKSETPSITRDVSGEGVQQALLKILEGTKVNVPPKG
                     GRKHPQQDFIEMDTKNILFIAGGAFFGLEKIIKERMDKKSIGFDVELGKKQDESTIFD
                     FTTPQDLLKYGLIPELIGRMPVICTLHELSEDALIDILVKPKNAICKQYQKFFEMDGV
                     RLEFSEEALKEIARTAIKQKTGARGLRSIMEKFMLKIMFSIPDRSDIESCIITPEVVT
                     LAQEPKFGYIKPLNSRNKVANEK"
     gene            1433..>1584
                     /locus_tag="PLG69_08120"
     CDS             1433..>1584
                     /locus_tag="PLG69_08120"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HPI43509.1"
                     /translation="MTAEVTIPRREKLFSALEEKEAIIIFAAEEEKLEKFKQDNDFLY
                     VTGLQIP"
BASE COUNT          563 a          259 c          333 g          429 t
ORIGIN      
        1 gaacggaatt attttatgag tgctgaagaa gcaatggaat acggtattat agatgaagtg
       61 atttcagtgc gaaaatagct tgactaaatt taagcagtca gataaaaatg acttacagaa
      121 agcggataaa attttgaagt aaagaaagta aattaaaaag gaggattcct tggataaata
      181 cagaacatta acctgttcct tttgcggaag aggcgaacat gaagtaaaaa atctgattag
      241 aggtattgcg ggcaacattt gtgatgaatg tattgtaatg tgccacaata taattgattc
      301 cagtaaagaa gacataaaac aagaggattt tgaagcaccc attcccagta gaataaaggc
      361 atatcttgat caatatgtaa tcggtcaaga cagtgccaag gaaataattt ctgtagcggt
      421 ctataatcat tacaagcgca tattcaaaca gaaatatgaa gatgatgtgg atttggaaaa
      481 aagtaatatc ttgatgattg gtcccacagg aagcggcaaa acccttattg ctcaaactct
      541 ggcaaggttt ttacaggttc ctttcgctat ttccgatgcc acaactttaa ccgaagcggg
      601 ttatgtaggt gaagatgtag aaaacatctt ggtgcgttta ctgcaaaatg ccaattatga
      661 tgttgccaaa gctgaacggg gaatcgtcta tatagatgaa attgataaaa tcagccgtaa
      721 atccgaaacc ccttccataa cgcgtgatgt tagcggtgaa ggagttcaac aagcgctact
      781 taaaattttg gaaggcacaa aagtaaatgt tcctcccaaa ggtggacgca aacatcctca
      841 acaggatttt attgagatgg acaccaaaaa tatcctgttt attgcgggag gagctttctt
      901 cggtttggaa aaaatcatca aagaaaggat ggataagaaa tccattggtt ttgatgtaga
      961 attgggtaaa aaacaggatg agtccacaat ctttgatttt acaactcctc aagacctgtt
     1021 aaaatatggc ttgattcctg aacttattgg tagaatgccg gttatttgca ctttacatga
     1081 acttagcgaa gatgccttga tagacattct ggtgaagccc aaaaatgcta tctgcaagca
     1141 atatcaaaag ttctttgaaa tggatggagt ccgcctggaa ttttcagaag aagcattaaa
     1201 agaaatagca agaaccgcta taaagcagaa aacaggtgcc cggggtttgc gttctattat
     1261 ggaaaaattt atgctgaaaa taatgtttag tattccagat cgctctgata tagagagttg
     1321 tattatcact ccggaagttg taactctcgc ccaagaaccc aaatttggct atattaagcc
     1381 cctcaattct cgcaataaag ttgctaatga aaaataaaat ggagacccga taatgacggc
     1441 tgaagtaaca attccgcgcc gggaaaaact attttccgca ttagaagaaa aagaagccat
     1501 aattattttc gctgcggaag aggaaaaatt agaaaaattc aaacaggata atgatttcct
     1561 ctatgtaacc ggtttacaga ttcc
//
LOCUS       DAOPFL010000218         1576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_113988_length_1576_cov_52.449704, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000218 DAOPFL010000000
VERSION     DAOPFL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113988_length_1576_cov_52.449704"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..450
                     /locus_tag="PLG69_08125"
     CDS             130..450
                     /locus_tag="PLG69_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43510.1"
                     /translation="MILADRSDYFGQGKGKIDHKNKIIPVDELMPIIEDIGAISCYNI
                     VKDYISGNYNASAFEPDDDLDCRDPFLQQAYEHNLLSDSQIKEIYEFDEFVSLTRFPG
                     IIRI"
     gene            complement(1099..>1576)
                     /locus_tag="PLG69_08130"
     CDS             complement(1099..>1576)
                     /locus_tag="PLG69_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43511.1"
                     /translation="YQKSCKAEFPGCYVIETNYTDFSAEEIWKYYMNLSEVEAAFRSM
                     KTELGTKLIYHQKDGRIKVHLFYSILAYAILKSITYKLALEDVHISWQSIKQILQTYM
                     CSDTIFNTTDVYRLIVSRMAGAIRVRIQYSILQMFIELETNKQPNLRQKRRKSIPF"
BASE COUNT          514 a          288 c          285 g          489 t
ORIGIN      
        1 gcttagtgca atttataatc tctggtaggg gggtacttat accctttact agccacaata
       61 tgcttctggg caattttact ttacaacagc aattatattt tgcttcctta acaaaatgcc
      121 aatagatata tgatattagc agatagaagt gattattttg ggcaaggcaa gggaaaaatt
      181 gatcataaga acaaaataat acctgtagat gaactgatgc ccataattga agatattgga
      241 gccataagct gttataacat tgtgaaagat tatatttcag gtaactataa tgcctcagcg
      301 tttgagccag atgatgatct tgattgtcgt gatccttttc tccaacaagc ttatgaacat
      361 aatctgcttt cagatagcca aattaaagaa atatacgaat ttgatgaatt tgtttctctc
      421 actcggttcc ccggaatcat aagaatttaa agagcggctt aaaccccatt cttttgctac
      481 caataaaact acaaaacgaa atgagtttta tagtaacgac agatactaac aacgggtttt
      541 gaacccggcg caataaaatc gctatgaaag agaaaagttc cggaggaacg gcagatacta
      601 acaacaggtt tcaacccggt gataagaaaa gaacatacaa aactatattt attggggaaa
      661 aaagttccat aggaacgaca gatactaaca acggttttca acccggtgat aagaaagcag
      721 gagagtcatt atctcctttt ccgagaaaga ggggttgtct cacaacctct caaaagtcca
      781 tattttgcca ttttcaggta tacaaagatg tatcctaaag aggagattgt tccttaaaat
      841 gccttatatt tactgtctca agactaactt ttagtttcgg gacagccccg acagatacta
      901 acaacgggtt tcaacccggt gataagaaag caggagagtc attatctcct tttccgacaa
      961 agagttccgg aggaacgaca gatgttagcc caagtttcgc aattatcaag ctaaagtatt
     1021 gttaaataag acaaattaat ttttttgcac actacaatct atatttttgt gataccgtat
     1081 tttgggaaac cttgatattc aaaagggaat agattttctt cgcttctgcc tcaggttggg
     1141 ttgtttgttt gtttctaact ctataaacat ctgtagtatt gaatattgta tccgaactct
     1201 aatagctcct gccatccgtg agacaatgag tctataaaca tctgtagtat tgaatattgt
     1261 atccgaacac atataggttt gcaggatttg tttgatgctt tgccagctaa tatgaacatc
     1321 ttcaagagcc agtttatagg ttatactttt cagaatagcg tacgctaaaa tggaatagaa
     1381 cagatgtacc tttatccgac catctttctg atgataaata agctttgttc ctaattcggt
     1441 tttcatactt cggaaggcag cttctacttc actcaaattc atataatatt tccagatttc
     1501 ttcagcactg aaatctgtat agttggtttc aattacataa caacccggaa actcagcttt
     1561 gcaggatttc tgatac
//
LOCUS       DAOPFL010000219         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_114683_length_1570_cov_76.059406, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000219 DAOPFL010000000
VERSION     DAOPFL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114683_length_1570_cov_76.059406"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PLG69_08135"
     CDS             <1..1043
                     /locus_tag="PLG69_08135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPI43512.1"
                     /translation="ACDKNPYLVYEHHQDGFPVKLICYPYWQKYFLTSLITYRILALP
                     AIKRCYKRFQPDIIHIHDYRHIPELFWLKPWLNKLSVPKYLTLHNIRTHPERLKNNPR
                     IGFYRWALKKTLTGWEHIFTVNSKIAKWVKQYNPASKVTIIGNAISELAEVDPQLIEP
                     YKNILLADYFHLISIGNLFTEKGFSYLIEAISLLKKEGLQVQLIIAGDGDRRKELENQ
                     VKTNNLNKELIFLGRIENKLLRNILPLFDLFVLASYSETFGIVFLEAMFAGLPVIGIK
                     EEGIYNLAEDGKQALFAEPKNSKDLAAKIKLLMLNPLLREEIAKAGQRLVQEKYMLKE
                     LIKRIIAVYEQK"
     gene            1030..>1570
                     /locus_tag="PLG69_08140"
     CDS             1030..>1570
                     /locus_tag="PLG69_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43513.1"
                     /translation="MNKNKILMIINEFPPTGQSGVQRPLKFVKYAVKAGWEVHIIAPK
                     KPVRKVVDYSLLQEIPKEAHIYRVAGLGIKTPDESKMVNAHFAETAPPSKIERAFWRL
                     AKLVNDFLFPYDKQIGWMPFAYYTACKIIKKQQIRNIYITAFPYSAFLVGLALKKKYK
                     DKIFWVADYRDSWQFGIMIE"
BASE COUNT          536 a          260 c          306 g          468 t
ORIGIN      
        1 ctgcttgtga taaaaatcct tatcttgtat atgaacatca tcaggatggc tttccagtaa
       61 aactaatctg ctatccctat tggcagaagt attttttaac ttccctgata acatatagaa
      121 tattggcttt accagccata aaaagatgct ataaaagatt tcaaccggat ataattcata
      181 ttcatgatta ccgtcatatt ccggaattat tttggttgaa gccctggtta aataaacttt
      241 ctgttcccaa gtatctcact ttacataata tacggactca tccggaacgg ctgaaaaata
      301 acccccggat tggtttttac cgttgggctt taaagaaaac actaactggt tgggagcata
      361 tttttaccgt aaatagcaaa atagctaaat gggtaaaaca atataaccct gcttccaaag
      421 taacgataat aggcaatgct atttcagagc ttgctgaagt tgatccgcaa ttaatagagc
      481 catataaaaa tattctgtta gctgattatt ttcatcttat cagcataggt aacctgttta
      541 ccgagaaggg cttttcatat ttgattgaag caatatcctt attaaaaaaa gaaggacttc
      601 aagttcagct cattatagca ggggatggag atagaagaaa ggaactggaa aatcaggtta
      661 aaacaaataa cttgaacaaa gaattgatat tccttggtag aattgagaat aaactgttaa
      721 gaaatatcct gcctttgttt gatttatttg ttctggcaag ctatagtgaa acttttggca
      781 ttgtatttct ggaagcaatg tttgccggct tgcctgtaat tggcattaaa gaagagggta
      841 tctacaattt ggcagaagac ggtaaacaag cattatttgc agagcccaaa aacagtaaag
      901 acctggctgc taaaattaaa ttactgatgc tgaatccgct tttaagagag gagatagcta
      961 aagcaggaca aagattggtg caagagaaat atatgctaaa ggaacttatt aaaagaataa
     1021 tagcggttta tgaacaaaaa taagatactg atgattatca acgaatttcc tcccacaggt
     1081 caaagcggag ttcaaagacc tttgaaattc gttaaatatg ccgtaaaagc cggatgggaa
     1141 gtgcatatta ttgctccaaa aaagccggtg agaaaagtgg tagattattc ccttctgcag
     1201 gaaataccca aagaagcaca tatttacaga gttgctggct taggcattaa gaccccggat
     1261 gaaagcaaaa tggtaaatgc ccattttgcg gaaacagctc caccttccaa gatagaaaga
     1321 gcattttgga gattggcaaa gctggtaaat gattttcttt tcccttatga taaacaaatc
     1381 ggctggatgc cttttgccta ttatactgct tgtaagatta ttaaaaagca acagattaga
     1441 aatatttaca tcactgcttt tccctattct gcttttctgg ttggtttggc tcttaagaag
     1501 aagtataagg acaaaatttt ctgggtagcc gattatagag attcctggca gtttggcata
     1561 atgatagaaa
//
LOCUS       DAOPFL010000220         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_114925_length_1568_cov_109.723067, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000220 DAOPFL010000000
VERSION     DAOPFL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114925_length_1568_cov_109.723067"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            798..1559
                     /locus_tag="PLG69_08145"
     CDS             798..1559
                     /locus_tag="PLG69_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43514.1"
                     /translation="MTLGDGTMRIDPKKLEKYLRYFFMLLTCLVIGYNLYLRQWETLW
                     SAVMTLLLFMLPTVLEKRIAITIPTSMQIIILLFIFASMYLGEIHRYFYRYRWWDRML
                     HTASAVILSYSGFILIYSMNKDYKVDVKLSPKFMALFSFCFAIMVCALWEIFEYTVDA
                     IFGVDMQKARNLEKVYGFFDTRLGVKDTMKDLIIDAIGAYVVSLIGYFHLKRKKDEDS
                     AFWSLHKQFMKDNPELFGMAEEENKIDMKMNRIKK"
BASE COUNT          482 a          234 c          315 g          537 t
ORIGIN      
        1 tggtactgca cgggcaactg tgtggaagag taggtcgccg ccatcgctaa cggcgaattt
       61 tttgaagtcg agaagtagag aagtttaatt tgcgccccta tgattttatt ctttacgata
      121 tctttggtgc cacctatgat tcgtgacgac cttaaacttt atctctcaca gatttcacga
      181 ataacacaga taaaaaattt agtttacgca gatttcgcag agtattattt aataataatc
      241 attatatttt aatccgattg atccagtttt ttgcattcta tctttttgtc gtcacgagtc
      301 ctcgctgtaa atgaatttgt ggcttattta taatttatga tgctgtgacg actcctgact
      361 tgcgtttttt tcgttttgct tttctaaata cctcaagacc tttccgtcct tgccatcagc
      421 acacaatcaa acaatgatgt acctatcatc cactcatcat tttggatggg ttgatgatgg
      481 gtagcgagtt aggaagaaac aaagaaaagg gataaatcgc attatcctat aatataattt
      541 ccctattgaa aatacagtta ttttgacaga acttgtccag attcctgctt ttgcggaatg
      601 acaaaggaat tttccttcaa aattcacatt agaaagggag gtgatgaatc tttagcttga
      661 caactggtta caaattatta taaatgcact aaaaaagatt ggaacatcta tttatcaaat
      721 tgaaaagaga gttattatat taaatattgt ttggatttct gcttgcgcgg gaatgacaag
      781 acatttatca atagtaaatg acattaggag atggaacaat gaggatagac ccgaaaaagt
      841 tagaaaaata tctgcgctat ttctttatgc tgttaacctg tttagtcatt ggctacaacc
      901 tttatttgag gcaatgggag acactttggt cagcggtaat gactttgttg ctgtttatgt
      961 taccgacggt gctggaaaaa agaattgcta taacaattcc tacttccatg caaatcataa
     1021 ttttactatt tatatttgcc tcaatgtact tgggagaaat tcaccgttat ttttatcgtt
     1081 atcgctggtg ggacaggatg ttgcatacgg catccgcggt aattttaagc tattcaggtt
     1141 ttattttaat ttattctatg aataaggatt ataaggtaga tgtgaaatta agtccgaaat
     1201 ttatggcgct gtttagtttt tgttttgcga taatggtttg tgccctatgg gaaatttttg
     1261 aatataccgt tgatgccatt tttggggtgg atatgcaaaa agcacggaat ttggaaaagg
     1321 tctacggttt ttttgatact cgtttagggg ttaaggatac aatgaaggac ttgattatag
     1381 atgctattgg cgcgtatgtt gtctctttga tcggatattt tcatttgaaa agaaaaaagg
     1441 atgaggattc ggctttttgg tcattgcata aacagtttat gaaagataat ccggaacttt
     1501 tcggaatggc tgaagaagag aataaaatag acatgaaaat gaacagaata aaaaaataaa
     1561 atagatta
//
LOCUS       DAOPFL010000221         1567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_115024_length_1567_cov_123.693122, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000221 DAOPFL010000000
VERSION     DAOPFL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115024_length_1567_cov_123.693122"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1567)
                     /locus_tag="PLG69_08150"
     CDS             complement(<1..>1567)
                     /locus_tag="PLG69_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPI43515.1"
                     /translation="CSPMPNYTIRLKHTTQTTLTTTFEVGDYTTVWTAPEFMPTTGWN
                     VHNFTTPFNWDGASNLLVDIVTTLITGNFTQNASVFYSTTTFNSSLRFQSDSTDASTS
                     ATGTVSTNRSNMQFIMAPLIVTNPPNPAILVSPANGATLVSPSANLNWVSGGGAPSGF
                     KLSLGTNNPPTNILNNQNLGLVTTYDPPQDFDLNTTYYWKVTPYNAMGDAVSCPVWSF
                     TTHGEPIVNSLPYSQNFDLVIPPALPFDWTSIVQAAVTTAYVDTYASTTYAHSQPNCV
                     RLYNSTDASATVMLIAPVLGDALDPHSVRIKFWARSSSAGYPLSVGVMTNPTDPATYQ
                     EVQNIALTTTITEYIVDLTTYTGNGRYITFKHGGGSSGTLYLDDISFELISPNDLAAL
                     SITGNVTPSVGNATNYTITVKNWGTASQSAYIVKLMSGDTELASAPGPTIAAGATATA
                     VVTWTPTAEGPMSIYGKVVLGSDVNPLNDNSPVMNISVMPPGATVITIGEGNLNEGVP
                     WEFFYKNSLFQTLY"
BASE COUNT          498 a          294 c          349 g          426 t
ORIGIN      
        1 aatacagagt ttgaaataag ctgtttttgt agaaaaattc ccagggaacg ccttcattta
       61 gattaccctc accaatagtt attacagtgg ctccaggagg cataacggaa atattcatta
      121 ccggagaatt gtcgtttaaa ggattaacat cactaccaag aactactttt ccatagatgc
      181 tcattggtcc ttctgcagta ggagtccatg ttacaactgc agtagcagta gctccagctg
      241 caatagttgg tccaggagca cttgccaatt ctgtatctcc gctcatcagt ttaactatat
      301 aggcactttg ggatgcagtt ccccagtttt taaccgttat agtatagtta gtagcattac
      361 ctacactggg agtaacattt ccggttatag aaagagcagc caaatcattt ggtgaaatta
      421 attcaaaact gatatcatca agatataaag tcccgctgga accgcctccg tgtttaaaag
      481 ttatataacg tccatttccc gtgtaagtag tcagatcaac aatatattcg gttattgtag
      541 tagtaagagc aatgttttgc acttcctgat aggtagcagg atcagttgga ttggtcataa
      601 ctcctactga aagcggataa ccggcactgc tggaacgagc ccagaattta attctgaccg
      661 aatgcggatc aagagcatct cctaaaacag gagctattag cataaccgta gcactggcat
      721 ccgtagaatt atagagacgg acacaatttg gttgactgtg agcataagta gtggaagcat
      781 aagtatctac ataagcggta gttactgctg cttgcacaat acttgtccag tcaaaaggta
      841 aagctggagg aatgaccaaa tcaaaatttt gggaataggg aagggaattt acaatcggct
      901 caccgtgagt agtaaaactc cagacaggac agcttaccgc atctcccatt gcattatagg
      961 gagtaacttt ccagtagtag gtagtattaa gatcaaaatc ttgaggggga tcataggtag
     1021 taaccaagcc cagattctga ttgttcaata tgtttgtagg aggattatta gttcccagag
     1081 aaagtttaaa accggaagga gcacctccac cgctaaccca atttaaattt gccgaaggac
     1141 taaccaaagt agctccattg gcaggagaaa ctaaaattgc cggattgggt ggattggtaa
     1201 ctattagagg agccattatg aattgcatat tggaacgatt ggtagaaacc gttccggttg
     1261 cagaagtaga agcatcggta ctatcgcttt gaaagcggag agagctgtta aaggtagttg
     1321 tagaataaaa aacagaagca ttctgagtaa aatttccggt tatcaaagtg gtaacaatat
     1381 ccacaaggag attggaagct ccatcccaat taaaaggagt agtaaaatta tgcacattcc
     1441 aaccagttgt aggcataaat tccggggcag tccaaacggt tgtataatcc cctacttcaa
     1501 aagtagtagt gagagttgtt tgagttgtgt gttttaacct aatcgtgtag ttcggcatcg
     1561 gactaca
//
LOCUS       DAOPFL010000222         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_116013_length_1558_cov_102.027944, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000222 DAOPFL010000000
VERSION     DAOPFL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116013_length_1558_cov_102.027944"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1042
                     /locus_tag="PLG69_08155"
     CDS             122..1042
                     /locus_tag="PLG69_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HPI43516.1"
                     /translation="MPKVLIATEKPFAADAVQKIKAELEKAGYEYSFLENYTDKSELH
                     NALADAEAVIIRSDIMDEEAFNSAPKLKIVVRAGAGYDNIDLKAATAHNVVAMNTPGQ
                     NSNAVAELAIGMMIYIARNKFNGKSGTELSGKILTLYGFGWVARYVAKIAKGIGMDVY
                     AYDPYLQDDVMLKEGVKPLHSVEDVFKLGDYISLHIPVTEETKKCVNWNLLSLVKEDA
                     VLVNTARKEIIDEEALLKVFAEKKKFRYVSDVAPDNSAEILELYPDRVFFTPKKMGAQ
                     TSEANTNAGIAAAKQIIAFFEKGDTTYKVN"
BASE COUNT          505 a          269 c          335 g          449 t
ORIGIN      
        1 atgcatgcaa tggctcctaa tgttgcagga gttgttggct cggcaattgc tgcaggtgtc
       61 cttttaggaa ttttaggaaa atgaaacaaa ataaacacaa caaaacatat aggaggcaaa
      121 aatgccaaaa gtcctgattg caaccgaaaa accctttgcc gcagatgcag ttcagaaaat
      181 taaggcagag cttgaaaaag ccggttacga atactccttt ttggagaatt ataccgataa
      241 aagtgaactg cataatgcct tagctgatgc ggaagcagta attatccgca gtgatattat
      301 ggatgaggaa gcttttaatt ccgctcccaa acttaaaatt gtagtacgtg caggtgccgg
      361 ttatgacaat attgatttaa aagctgctac tgcacataat gttgtagcta tgaatactcc
      421 agggcaaaat tccaatgctg ttgccgaatt ggcaatcgga atgatgattt atattgccag
      481 gaacaaattt aacggcaaat ccggaacgga actttctggt aagatactta ctctgtatgg
      541 ttttggctgg gttgcccgtt atgtagcaaa aattgctaaa ggcatcggaa tggatgttta
      601 tgcttacgat ccttatctgc aggatgatgt gatgttgaaa gaaggcgtta aacccttgca
      661 tagtgtagaa gatgtcttca aactggggga ttatatttct ctacatattc cggttactga
      721 agaaactaaa aaatgcgtta actggaatct tttatctttg gtaaaagaag atgccgttct
      781 tgttaataca gcacggaaag aaattattga tgaagaagca ctacttaagg tgtttgccga
      841 aaagaaaaaa ttccgttatg tgagtgatgt agctccggat aatagtgctg aaatattaga
      901 actgtatcca gaccgtgttt tcttcactcc taaaaaaatg ggagcccaaa ccagcgaagc
      961 aaatactaat gccggaattg ctgccgccaa acaaattatt gccttctttg agaaaggcga
     1021 tacaacctac aaagtaaact aaaaactacc ctcctcctcg attacaacgg aaagcttatc
     1081 ttcagctttc cgttgttttc tctctgaaga ataatctgca ataaaaaggt tgagaaagtt
     1141 gatagagttg attgggttga gaaggtttag agggttgatg gggtttagaa ggttgagaag
     1201 gtttagaagg ttgaaatttt ttcaaagccc aaagggcgac acaatcatag cgatgggagc
     1261 gtaagcccct cgtaaaaaag tacgaaacaa ccatctatat tttttcacag ttccccggta
     1321 tatatatctt gaaaatcctt ttcatcccct tcctcaagga gctaataaat cttgatagtt
     1381 atttgcttga tatatagtaa attctgtaac gactccgtaa cacttcccta acacttccgt
     1441 tgtttcgtta cggagttgtt acttggttgt tactttgtta taagaagatg aagcaagctg
     1501 caaaagataa ttaattaaag gcgaagcagt taacagaagg gcggtaaatg ataatgtt
//
LOCUS       DAOPFL010000223         1551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_116822_length_1551_cov_97.630348, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000223 DAOPFL010000000
VERSION     DAOPFL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116822_length_1551_cov_97.630348"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLG69_08160"
     CDS             complement(<1..521)
                     /locus_tag="PLG69_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPI43517.1"
                     /translation="MFKNKIKPFLLLLLFSVAFYLNADYYDSVINLTGDDLFYGLRNL
                     ISNNTNTSYDASKVVLYQTLDNFNGYVTCVYTGQEYYVGFDYTGSTNPNTEHTYAQSW
                     FNGTDTTKKKSDLHHLFITNSVVNSSRGNYPFDVVANHNTANVYYTYTPWQSYRGYNA
                     QNRMVFEPADEFKG"
     gene            complement(534..1142)
                     /locus_tag="PLG69_08165"
     CDS             complement(534..1142)
                     /locus_tag="PLG69_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPI43518.1"
                     /translation="MNSIIIETFQEAQELISQVLNRENILPQIEAMAKQMADVLLNKG
                     KLIACGNGGSMCDAIHFAEELSGRFNKDRQALSAIALSDPGYLSCVANDYGWDNVFAR
                     GVDAFALKGDLILGISTSGNSTNIIKALKTAKKKECFTASLLGKEGGKLKGFCDYEII
                     VPSNNTARIQEIHGIIIHLLIELIEKELFTSQEENPQAPLFC"
     gene            complement(1156..>1551)
                     /locus_tag="PLG69_08170"
     CDS             complement(1156..>1551)
                     /locus_tag="PLG69_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPI43519.1"
                     /translation="GTRLVEDGDLLNYVNDHRIPLEICLTSNLHTKAVPDIRSHPIDF
                     YTDYGLRVTLNTDNRTISNTTVTDEYMLAINELGLDYPAVKYIILNGFKSAFLPYKER
                     VRLINQILKEIDEIEEQELKTRVKVKENL"
BASE COUNT          459 a          320 c          277 g          495 t
ORIGIN      
        1 cccttaaatt catctgcggg ctcaaaaacc attctgtttt gagcattata accccgataa
       61 ctttgccacg gtgtgtaagt ataataaaca ttggcagtat tatgattggc aactacatca
      121 aaagggtaat ttccccggga actgtttacc accgaattag ttataaacag atgatggagg
      181 tctgatttct ttttagtagt gtcagttccg ttgaaccagc tttgggcata agtatgttcc
      241 gtattgggat tggtactacc ggtataatca aagcccacat aatattcctg tccggtataa
      301 acgcaggtta catagccgtt aaaattatcc agggtttgat agagaactac cttggaagca
      361 tcgtaactgg tattggtatt attggaaatc aagttccgca aaccataaaa taggtcatca
      421 ccggttaaat taataacgct atcataataa tccgcattca ggtaaaaagc aacactgaat
      481 aacagtaaga gcaaaaaagg ttttatctta ttcttaaaca tttttccctt attttagcag
      541 aataatgggg cttggggatt ttcttcctga ctggtaaaca gctctttttc tatcaattca
      601 atcagcagat ggataataat tccgtgaatt tcctgaatgc gagctgtgtt attagaagga
      661 acaatgattt cataatcaca aaatcctttc agtttgcccc cctcttttcc caaaagagaa
      721 gcggtgaaac attccttctt cttagcggtt tttagggctt taataatatt ggtggaatta
      781 ccgctggtag aaattcctaa aatcaaatcc ccttttaagg caaaggcatc cactcctctg
      841 gcaaaaacat tatcccagcc ataatcatta gctacgcagc ttaaatagcc aggatcgctt
      901 aaagcaatag ccgaaagtgc ttggcggtct ttattaaaac gccctgaaag ttcttccgca
      961 aaatgaatgg catcacacat tgaaccgcca tttccgcagg caatcagttt tcccttattt
     1021 agcaaaacat cagccatttg ttttgccatt gcctcaattt ggggaagaat gttttccctg
     1081 ttcagcactt gggaaatcaa ttcttgtgct tcttggaagg tctctataat gatgctgttc
     1141 aagtttagtt gctccttaca gattttcttt cactttcacc ctcgttttca attcctgttc
     1201 ttcaatttca tcaatttcct tcagaatctg attgatcaaa cgcactcttt ccttataggg
     1261 cagaaaggca cttttgaaac cgtttaagat gatatatttc acggcaggat aatccaaacc
     1321 gagttcattt attgcaagca tatattcatc ggttacggta gtattgctga ttgttctatt
     1381 atccgtattg agcgttaccc gcaagccata atctgtataa aaatcaatag gatgactgcg
     1441 aatatcggga acagctttag tatgcaaatt gctggttaaa caaatctcta aaggaatcct
     1501 gtggtcattt acataattta acaggtctcc atcttccacc aaacgtgttc c
//
LOCUS       DAOPFL010000224         1548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_117166_length_1548_cov_98.056263, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000224 DAOPFL010000000
VERSION     DAOPFL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117166_length_1548_cov_98.056263"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLG69_08175"
     CDS             complement(<1..887)
                     /locus_tag="PLG69_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPI43520.1"
                     /translation="MFENLSERLDKIFRNLRGKGYLTEDNIKAGLREVRMALLEADVN
                     FRIVKNFIAEVEKRALGVEVMKSLTPGQQVVKIVYEQLVNLMDTEGFQFKVYNNRLTK
                     VMLVGLQGSGKTTACAKLAAFYRKKGIKPMLVACDVYRPAAVDQLKILGKQIGIPVVS
                     EDTKNVLKIADSALSMAEKEMVNLLIFDTAGRLHIDEPMMEEVARLKTHIKPDYIFFV
                     ADATTGQDAVTIAKDFYDKLAFDAVILTKLDGDARGGAALSIKAVTGKPVAFVGIGEK
                     ISDLEIFYPDRMASRILGMG"
     gene            complement(906..>1548)
                     /gene="rpe"
                     /locus_tag="PLG69_08180"
     CDS             complement(906..>1548)
                     /gene="rpe"
                     /locus_tag="PLG69_08180"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPI43521.1"
                     /translation="QIAPSLLAADFGNLDSEITSLEAAGADILHLDIMDGHYVPNLSF
                     GFPVIRTIRFLSKLPLDVHLMVTNPDFHISLLLDMDVQWVSFHQETFYHSHRLIQTLK
                     EKGIKAGLALNPGTPIGTLEALLPDLDYILVMSVNPGFSAQDFIPSAIYKIWDLYNLR
                     KKNNYRYLIEVDGGVTAANAKALKEAGADILVSASYIFSSHDYSQAISALRNA"
BASE COUNT          478 a          352 c          282 g          436 t
ORIGIN      
        1 cccattccca aaatacggct tgccattcta tcgggataaa aaatttccag atcgctgatt
       61 ttttccccga tgccgacaaa agcaaccggt tttcccgtaa ctgctttaat ggataaagct
      121 gctccaccgc gggcatcgcc atccagttta gttaaaataa cggcatcaaa ggcaagttta
      181 tcgtaaaagt ccttagcaat tgtaacggca tcctgtccgg ttgttgcatc tgccacaaaa
      241 aatatataat ctggttttat atgtgtcttc aagcgtgcca cttcttccat catcggttca
      301 tcaatatgaa gacggcctgc agtatcaaaa ataagcagat taaccatttc cttttctgcc
      361 atagataaag cggaatccgc tattttaaga acatttttgg tatcttcgga aacgacggga
      421 ataccaattt gtttacccag aattttaagc tgatcaacag ctgcaggacg gtaaacatca
      481 caggcaacaa gcatcggttt gatgcctttt ttccggtaaa aagcggcaag tttggcacag
      541 gcagttgttt ttcccgaacc ctgcaaacca accagcataa ctttagtgag gcgattgttg
      601 tagaccttaa actgaaaacc ttccgtatcc ataaggttaa caagctgttc ataaacgatt
      661 tttacaacct gctgacccgg ggtaagggac ttcataactt ctacacccaa agcccgtttt
      721 tccacttcag caatgaaatt cttcacaatt ctgaagttta catcggcttc caaaagcgcc
      781 attcggactt cgcgcaaacc cgcttttatg ttatcttcgg ttaaatatcc ttttccccgc
      841 aggttacgga aaattttatc caagcgttca gataggtttt caaacattat tgatccttaa
      901 tatgttcagg catttcttaa agcagaaatt gcctgactat aatcgtgact gctgaaaata
      961 taggaggcag aaaccagaat atcggctccc gcttctttta atgctttcgc gttagcggca
     1021 gtaactcccc catcaacttc tatgagatag cggtaattat tctttttccg caagttatat
     1081 aaatcccaaa ttttatagat ggcagaagga ataaagtcct gagccgaaaa accaggattt
     1141 acactcatca ccaaaatata atccaaatca ggcaaaagag cttccaaagt tcctatagga
     1201 gttccaggat ttaatgccaa cccggcttta atgccttttt cttttaatgt ctgaataagc
     1261 cggtgactat gataaaatgt ttcctgatga aagctgaccc attgcacatc catatccagc
     1321 aaaagggaaa tgtgaaaatc cggattggta accattaaat gaacatccaa aggcagctta
     1381 cttaaaaaac gaatagttct aattactgga aacccgaaac tcaaattggg cacatagtga
     1441 ccatccataa tatccaaatg caggatatca gctccagcgg cttccagaga agtaatttcg
     1501 gaatcaagat ttccaaaatc cgctgctaag agtgaagggg ctatttgc
//
LOCUS       DAOPFL010000225         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_122295_length_1505_cov_102.636552, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000225 DAOPFL010000000
VERSION     DAOPFL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122295_length_1505_cov_102.636552"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1505
                     /locus_tag="PLG69_08185"
     CDS             <1..>1505
                     /locus_tag="PLG69_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPI43522.1"
                     /translation="KIEEILAQSKFMQRVLKLPLKDRSIQIYHLNQSARALLAVHLWK
                     HTGKNIILVSQDDIIAEDIWDDLCVLIGKENAHYLPDYEILPYEERSPHYSIRATRME
                     TFLATLNNNKSAIYSLSARALGRYLPAKKFLSRHILHLKQGMECEPDTLLHNLYDMGY
                     EIQYQVSKVFQAAKRGGIIDIFSPPLTNPVRLEFWGDEIIGMRVFSVTTQRSLEQYLT
                     ELTILPARELSLSDVDSGSPIIAKIRNQGFFEGIENYYALLCNELQTFADYFEKDNRI
                     LVWNNFYYLQEEYETLFEQALTTWQKEAKIQGQGRVPRPEQMFADWDVLTELKEKSEK
                     IYLSQSEFELSFPTLNMRAPFASQPNFESDLGLLAETLFSRNKEGWQNILLFDNQSQA
                     KRMQQSIGKVPFIEFFGVLHEGFSLEDCHLNVWTDHEIFNRYKSRRYTPRYAPGETIV
                     NYEDLKPGDYVVHIDHGIGVFEGLKIIRLDGSDVECLVLRYANDDRVYVPT"
BASE COUNT          454 a          273 c          311 g          467 t
ORIGIN      
        1 taaaatagaa gagatattag cccaaagtaa atttatgcaa agggtgctca agcttccctt
       61 aaaagatagg tctattcaaa tttaccacct caatcaaagt gcccgggcat tattagcagt
      121 tcatttatgg aaacataccg gaaaaaatat aatccttgtt tctcaggatg atatcatagc
      181 agaggatatt tgggatgacc tctgtgtact tatcggaaag gaaaatgcac attatttgcc
      241 cgattatgag attttgccct atgaagaacg ttctccgcat tacagtattc gggcaacgcg
      301 aatggagacc tttcttgcta cccttaataa taataaaagt gctatttatt cgctttcggc
      361 aagagcttta ggacgctatt tacctgccaa aaaatttctc tcccgtcata ttttacatct
      421 taaacagggt atggaatgtg agcccgacac cttacttcac aatttatacg atatgggtta
      481 tgaaattcaa tatcaggtta gcaaggtctt tcaggcagca aaaagaggcg gtattataga
      541 tattttttca cctccgctaa ctaatcctgt gcgtttggaa ttctggggtg atgaaattat
      601 tggaatgcgt gttttttcag ttactacaca gcgaagtttg gaacaatact taacggaact
      661 gacaatttta cctgccaggg aactttcttt aagcgatgtt gatagtggtt caccgataat
      721 tgccaaaatt cggaatcagg gtttctttga aggaattgag aattattatg cattgctatg
      781 taatgaatta cagacctttg ctgattactt tgaaaaggat aatcgcattc tggtttggaa
      841 caatttttac tatcttcagg aagagtatga aacccttttt gagcaagcac ttaccacctg
      901 gcaaaaagaa gccaaaattc aagggcaggg aagagttcct cgcccggaac aaatgtttgc
      961 cgattgggat gttttaacag aattgaaaga gaaaagtgaa aaaatttatc tttctcaaag
     1021 tgaatttgaa ctctccttcc ccactttgaa tatgcgtgct ccttttgcaa gtcaacccaa
     1081 ttttgaatcc gatttgggac tgctggcaga aacacttttt tccagaaaca aagagggctg
     1141 gcagaatatt ttactttttg ataatcaaag tcaggcaaag cgtatgcagc aaagtattgg
     1201 caaagttcct tttattgagt tcttcggagt tttgcacgag ggcttttccc tggaagattg
     1261 ccatctaaat gtatggacag atcacgaaat ctttaatcgt tataaaagca gacgctatac
     1321 tccccgttat gcaccgggag aaactattgt aaattatgaa gacctcaagc caggggacta
     1381 tgttgtgcat atagatcacg gcattggtgt ttttgaagga ctgaaaataa tccgtttgga
     1441 cggttccgat gttgaatgcc tggttttgcg ttatgctaat gatgatcggg tttatgttcc
     1501 tactt
//
LOCUS       DAOPFL010000226         1503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_122547_length_1503_cov_110.317680, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000226 DAOPFL010000000
VERSION     DAOPFL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122547_length_1503_cov_110.317680"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(872..>1503)
                     /locus_tag="PLG69_08190"
     CDS             complement(872..>1503)
                     /locus_tag="PLG69_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPI43523.1"
                     /translation="FNQLTNAGVLVEDKLFATLDSTSRQLKLSTGNPVVLSDTVGFIS
                     KLPHHLIASFKATLMEVQDANLLLHIVDVSDERFEYYIQQVNSVLQQIGAETIPQILV
                     FNKIDNVDSILVSLLESRFPEGVFISAIQGINIDKLLAKMEEILLGNRILQLKIPYEK
                     TALVSKMHDIAEIISEDYKEDGIYLKVEISRDDRFLVEDYVLENEEGKN"
BASE COUNT          471 a          296 c          224 g          512 t
ORIGIN      
        1 agatttccgg aaggttgata tccttgtaaa cccctcgtaa aaatttaagt caggagtcat
       61 tagtgctttc aaaacttcgg cttcagaaat aactttactg gcgcttatgc ctcgtgacta
      121 cctattatca atatgaattt aactgatttt acgaattaac actgattatt ttataataat
      181 cccctatttt aacaacaact caatttcttt ctttattttt ctggaattgc ccatcatcaa
      241 aacacacggc taattatttt gctaatttac tatattataa tttgttatat tttcatcctt
      301 aggagcattg cttacccatt gcttacctat tactaatgcc attagcaatg ggtaagcaat
      361 gcttcagtaa tgttatttgg taagaacatt tttttgatag ttaaacccga ataaggcaat
      421 tgaggaaaaa tagattggca acttccgctt tgccaaagag tttgtagcac tctgaaaaat
      481 atatgtcctc tatttatagt gaaacagaat gcggcaatct aaatataaaa tttaactttt
      541 ctgcccaggg ttaaacttcc accataatat ctgtcattct tacagaactc tttctctttc
      601 atagcgatat ctttttgcag ggttaaaacc tatcgttatc taactttcat tgtaaaggga
      661 tttttactaa aatataagct taatgattag ggaaaaaata ttaaccatta tttttacagc
      721 gtcatttggg taaaaaaggt ggagaaggtt tagatgcttt ctgctctttg ctcttaacca
      781 aaatccttac gacctattag caacaaggat ttaactgatt ttacggatta acacggattt
      841 ttttattata ttcccctatt ttaacaacac ctcaattttt tccttcttca ttttccagca
      901 cataatcctc aaccaaaaac ctgtcatccc tgctgatttc cactttcaga tagattccat
      961 cctctttata gtcctcactt ataatttctg caatatcgtg catcttagaa accagagccg
     1021 ttttctcgta aggaattttt agttgtaata ttctgtttcc taaaagaatt tcttccattt
     1081 tggcaagcaa tttgtctata tttatgcctt gaatagcgga aataaacact ccttccggaa
     1141 aacggctttc caacagagac accaatatgg aatccacatt gtctatttta ttgaagacca
     1201 aaatctgcgg aattgtttca gctccaattt gttgcagaac cgaattcacc tgttgaatgt
     1261 aatattcaaa gcgctcatcg gaaacatcaa caatatgtaa cagcaaattg gcatcctgca
     1321 cttccattaa agttgccttg aaagaagcaa tcaaatgatg aggcagtttg gaaataaaac
     1381 ctaccgtatc cgatagcaca accggatttc ccgtggaaag tttcagctgt ctggaagtgg
     1441 aatccagggt ggcaaaaagt ttatcttcaa ccagcacacc ggcattagtt aactgattaa
     1501 aaa
//
LOCUS       DAOPFL010000227         1465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_127481_length_1465_cov_93.236879, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000227 DAOPFL010000000
VERSION     DAOPFL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127481_length_1465_cov_93.236879"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..>1465
                     /locus_tag="PLG69_08195"
     CDS             411..>1465
                     /locus_tag="PLG69_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPI43524.1"
                     /translation="MQKKITLGIEGMHCASCSARAEKALSQLKGVSEANVNLALEEAY
                     IVYDDKQLTLADFKQAIEKLGFKVKETEADKESEQIRQMQVAKKKMGLSWLITALVLL
                     LMIPDMVLGRAIISEQMDAWLMVILSLLAMIFPARQVYLSAYKAVKSGTANMDVLIAM
                     GTIASLLAAPLSLFIKDIVANSFAGIAAMIISFHLTGRFLEAKAKGTASEEIRKLIGL
                     GAKTAIILDAGTEKEIPLSQLKVGDIFIVKPGAKIPTDGIVIKGNSLVDESMATGESL
                     PVIKKPNDNVLGATINLDGYLEVQATKVGKETFLAQVIQMVSEAQHSKVPIQLLADKI
                     TAIFVPVVLCLAVIVFAV"
BASE COUNT          465 a          242 c          349 g          409 t
ORIGIN      
        1 aatcagcgaa atctgcgtga actgaaatca gaacttaacc cataaaaatc agtataaatc
       61 agcaaaatcc gacccatcct tattgctaat tttttgaggt tttcttagga gcattaggaa
      121 agcattgctt acccattacg gatggcatta gcaatgggta agcaatgggt aagcaatgct
      181 attaaggacg gagttgtaaa ccaatatgaa tttgttagtt aggcaatcag atgagagttt
      241 tccttgtgtt actgaatttt cgggatatta tatccataag acaaatttcc tgtggacaaa
      301 gtagtagcgc ttatgaatta tatttatttc gtttggaaaa ggaaaggaaa gaactttttc
      361 cgcagggttt ccggttcaaa caaaagaaaa taaaataagg ataaacaaga atgcaaaaga
      421 aaattacctt gggaattgaa ggaatgcatt gcgcttcttg cagcgcgaga gccgaaaaag
      481 cactgtcaca attgaaagga gtgagcgaag caaatgtaaa tctcgctctg gaagaagcat
      541 acattgttta cgatgacaag caattaacgc ttgctgattt taagcaggca atagaaaaac
      601 tgggttttaa agtgaaagag acagaggcag ataaagaaag cgaacaaatc cgccaaatgc
      661 aggtagcaaa aaagaaaatg gggctttcct ggctgattac tgccttggtt ctgctattaa
      721 tgattcccga tatggttttg ggaagagcaa ttattagcga acagatggat gcctggctga
      781 tggtgatatt gagtttgctg gcgatgattt tccctgcccg acaggtatat ctttctgcct
      841 ataaagcggt aaaaagcgga actgccaata tggatgtgtt aattgcaatg ggcacaatcg
      901 cttctttgct ggcagctcct ctttcgctgt ttataaagga tattgtggca aatagttttg
      961 caggaattgc tgcgatgatt atttcttttc atttaaccgg acgctttctg gaggcaaaag
     1021 ccaaaggaac tgcttcggag gaaatcagaa aactaatcgg tttaggtgct aaaacggcaa
     1081 ttattttgga tgcgggcacg gaaaaagaaa ttccgcttag tcaactgaaa gtgggggata
     1141 tctttatagt taagcccgga gcaaagattc ctaccgacgg cattgtgatt aaaggaaata
     1201 gtttagtgga tgagtcaatg gcaacaggtg aatcccttcc cgtaattaaa aagccgaatg
     1261 ataatgtgtt aggtgcaacc atcaaccttg atggctatct ggaagtgcaa gcgactaagg
     1321 ttggtaaaga gacctttctg gcgcaagtga ttcaaatggt aagcgaggca cagcattcta
     1381 aagttcctat tcaattgctg gcagataaga taaccgctat ttttgttcct gtggtgctat
     1441 gtttggcagt gattgttttt gcggt
//
LOCUS       DAOPFL010000228         1450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_129510_length_1450_cov_72.504659, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000228 DAOPFL010000000
VERSION     DAOPFL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129510_length_1450_cov_72.504659"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLG69_08200"
     CDS             <1..300
                     /locus_tag="PLG69_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43525.1"
                     /translation="VFTLQSIKSRIETRFRHYYPDDIGTEEDKDAAKVANAVILFLKV
                     LELNRNTGFIITTNLNSEELRNRYGDCLFDRIIGQLTILYLNPHSYRKSHAEIVK"
     gene            374..724
                     /locus_tag="PLG69_08205"
     CDS             374..724
                     /locus_tag="PLG69_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43526.1"
                     /translation="MKIPDPIMTGKRLQLIKERGVSDELRRTFDGVLVKSSGNYCLEF
                     KCGYNQLTEHQKDYRMKINAINNSFYIVRKIIKKGLNGRTGEIIYKLTTPKNKVIQSN
                     NLETIIDYIGGNNE"
     gene            717..971
                     /locus_tag="PLG69_08210"
     CDS             717..971
                     /locus_tag="PLG69_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43527.1"
                     /translation="MNNEKDRIICSLGKEYNESRLVGEMMEAYRIRQCYTCYRNYNHC
                     NYYNDAIDTVSADVHKGKKELPDPCPNYENKFTREKRKKK"
BASE COUNT          545 a          236 c          282 g          387 t
ORIGIN      
        1 gtttttactc tacagtccat taaatcaagg attgaaacaa gatttaggca ctattatcct
       61 gatgatatag gaacggaaga agataaagat gcggctaaag tagccaatgc agtaatcctt
      121 ttcttgaaag tgttagagct gaatagaaat acaggattca ttatcaccac gaatctgaat
      181 tcagaagaac tgagaaatag atacggtgac tgcctgtttg accgcattat aggtcaatta
      241 acgatacttt acttgaatcc gcattcttat agaaaaagtc acgcagagat agtgaaatga
      301 aaaaaataag aaaccagaaa aggcatttga agaggaattc gcgtggcgtg cgaaaataaa
      361 gaaatgcctt tatgtgaaaa tacccgaccc gataatgact gggaaaagat tgcagctgat
      421 taaagaaaga ggggtgtctg atgaattaag acgcaccttt gatggggttc tggtgaagag
      481 ttctggcaac tattgtctgg aattcaagtg cggatacaat cagctcacgg aacaccaaaa
      541 ggattataga atgaagataa atgcgataaa taattcattc tatattgtgc ggaaaattat
      601 caaaaaagga ttgaatggaa gaacagggga aataatatat aaattaacta cccctaaaaa
      661 taaagtgata caaagtaata atttagaaac tatcattgat tacataggag gtaacaatga
      721 ataatgaaaa agatagaatt atatgctcat tgggcaaaga atacaacgaa tcacgactgg
      781 taggagaaat gatggaggca tacaggatac ggcagtgcta tacctgctat agaaattata
      841 atcattgcaa ttattacaat gatgcaattg acacggtttc tgccgatgtg cataaaggaa
      901 aaaaggaatt gcccgaccct tgtccgaact atgaaaacaa attcacccga gaaaaaagga
      961 aaaaaaaatg aaaaagcagc aatcttatct atcattgaat gtatccttct agcaatcttt
     1021 ctcttatctg cgattgctat tattattcta ttacttcagt tgttcattat aaaaatagat
     1081 aatgcttcct atgttgcatt attcgctgcc ttggaaacat tctccatcgg tggcttaatt
     1141 gcgatagaat tttcatccta cagtccatta aatcaagaat tgaaaccgta gacaacaaag
     1201 gcaaataagc caacagtaga taattaatat cctaccttcc attactaaag tgaatatctc
     1261 caaagctgac aagatatggg taaagattgt gcggttgaaa gagaaatatg gttctacagc
     1321 agctaactat gaaattcaac tccagccaag caattctgct cctcagagga tagaatctct
     1381 cataataact gatcggtatc agaaatcctg caaagctgag tttccgggtt gttatgtaat
     1441 tgaaaccaac
//
LOCUS       DAOPFL010000229         1442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_130569_length_1442_cov_95.392213, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000229 DAOPFL010000000
VERSION     DAOPFL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130569_length_1442_cov_95.392213"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="groL"
                     /locus_tag="PLG69_08215"
     CDS             complement(<1..1142)
                     /gene="groL"
                     /locus_tag="PLG69_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPI43528.1"
                     /translation="MAKQMQYAHDARTSLKSGVDKLADAVKVTLGPKGRYVVLDRKFG
                     SPTITNDGVTIAKEIELENEYENMGAQLCKEVAEKTHDTAGDGTTTATILAQAIIEEG
                     LKHVTAGVNPMYLKRGLEKATKVVVDKIREFSKEIKSSEEIAQIAAISANNDPEIGAL
                     IAEAMDSVGREGIINIEEAKSIDTGMEKVEGMQFDRGYISPYFVTNAEKMIAELEEAF
                     ILLYDKKITALKELLPILQEVSQNGRPLLIIAEDIEGEALATLVVNKLRGVLNVVAVK
                     APGFGDRRKAMLEDIAVLTGATLISEEMGRKLDSATMSDLGRAKKIIVEKENTTIREG
                     AGNPEEIEGRIKQIRAQIEETTSDYDKEKLQERLAKLSSGVAVLRIG"
BASE COUNT          379 a          313 c          245 g          505 t
ORIGIN      
        1 ccgattctga ggacggcaac tccgctggaa agtttggcaa ggcgttcctg caatttttct
       61 ttatcgtaat cggaagttgt ctcttcaatt tgagctctta tttgtttaat tctaccttca
      121 atctcttcag gattaccagc tccttcgcgg atagtggtat tttctttttc cacaataatc
      181 tttttagcgc gtcccaaatc gctcattgta gcgctatcaa gttttctccc catttcttcg
      241 gaaattaaag ttgcaccagt taaaacggca atatcttcca gcatagcttt tctgcgatca
      301 ccgaaaccgg gtgctttaac agcaactaca ttcaggacac cacgcagttt attcactact
      361 aatgtggcaa gtgcttcgcc ttcaatatct tctgcaataa taagcagagg acgtccattt
      421 tgggaaactt cttgtaagat gggaagaagt tctttgagag ctgttatttt cttgtcgtat
      481 aaaaggataa aggcctcttc cagttcggca atcatctttt ctgcattagt gacaaaatag
      541 ggagagatat aacctctatc aaactgcatt ccttccactt tttccatacc ggtatcaatg
      601 gattttgctt cttcaatatt gataatgcct tctctaccaa cactatccat agcttcagca
      661 atcagagctc caatttcagg atcgttatta gctgaaattg cagcaatttg tgcaatttct
      721 tcgctgctct tgatttcctt gctgaattcc cttattttat ccactacaac tttagttgcc
      781 ttttccaaac cgcgtttcag atacatcgga ttaacgccgg cagttacatg tttcagacct
      841 tcttcaataa ttgcctgagc taaaattgta gctgttgtag ttccatcacc agctgtgtca
      901 tgggtttttt ctgcaacttc tttgcagagt tgagcaccca tattttcgta ttcattttcc
      961 aattcaattt ctttggctat ggtcacaccg tcgttagtga ttgtgggaga gccaaatttc
     1021 ctgtccaaaa caacatatct acccttagga ccaagggtaa cttttacggc atcagccagt
     1081 ttatccacac cgctttttaa acttgtgcgg gcgtcatgtg catactgcat ttgttttgcc
     1141 atcttgtttt cctccataat tttagtattt taattttggt tttcttagca gtcaatacaa
     1201 ttgagtgcta atattattat agccgaaatt ctgtcaagcg caaattaaaa attttttgtc
     1261 tttcgcgctt atttttctct tttcaatggg actgtcccga aactaaaact tagttatgag
     1321 agaataaata taaaacattt taaagaacaa tctcctcttg agaatatatc tttgtatacc
     1381 tgaaaatggt aaaaaatgga ctttttggaa attgtgagac aaccccgaca ggtattaacg
     1441 ac
//
LOCUS       DAOPFL010000230         1440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_130870_length_1440_cov_150.246931, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000230 DAOPFL010000000
VERSION     DAOPFL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130870_length_1440_cov_150.246931"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PLG69_08220"
     CDS             <1..1014
                     /locus_tag="PLG69_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPI43529.1"
                     /translation="GIKKYQWVSTPLALHGVIIKKDGSVLNICLGEKEDEPVFVIPDL
                     LPHLAGKEQYTKKLDEAIEAGKMNLVFSGMQEPGNEEKEAIKNYALRQLHEKYGITEA
                     DFQSAELQLVPAFKARHSGFDNSFVIGYGQDDRICAYNALMALINNLPDKPKRTMVVY
                     FSDKEEVGSQGNTGAQSIFIQDFISDLLAHNNEDNSSANLRKTFINSQILSADVTAAI
                     DPNYPNVHEKQNAVIFNYGIGISKFTGRGGKYSCNDANAEFIAKVIRIFNQAGVFWQS
                     GELGKVDEGGGGTIAYFLSNLGAEVLDCGVGLMGMHSLYELCSKADLYSTYLGYKAFL
                     QAD"
     gene            1018..>1440
                     /locus_tag="PLG69_08225"
     CDS             1018..>1440
                     /locus_tag="PLG69_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43530.1"
                     /translation="MKKYLLPVCFLLLISKLLSFQETLTLDQIITEIATEIQTGQPIF
                     LDINCGEWNPVLTQKLSTNLLEKGADLRYNLSETKTDISSLEEKKVPDLSALGLEKAL
                     LVQINLNIAWQETVKSSFFSYRTERYPVHIFEIRQIQLP"
BASE COUNT          450 a          266 c          300 g          424 t
ORIGIN      
        1 ggaattaaaa aatatcagtg ggtttctact cctctggctt tgcacggagt tattattaaa
       61 aaagacggct ccgttttgaa tatttgcctt ggggaaaaag aagatgagcc ggtttttgtg
      121 attcccgatc ttttgcctca tttggcagga aaggaacaat ataccaagaa gttagacgaa
      181 gcaatagaag ccggtaaaat gaatcttgtt ttttcgggaa tgcaagaacc aggcaatgag
      241 gaaaaagaag caatcaagaa ttatgcctta agacagctgc acgaaaaata cggcattacc
      301 gaagctgatt ttcaaagtgc ggaactgcaa ttagttccag cttttaaagc gcgtcattca
      361 ggttttgata attcttttgt aattggttat ggacaggatg atcgtatttg tgcctataat
      421 gccttgatgg ctttaataaa taaccttcct gataagccca aacgcacaat ggtggtctat
      481 ttttccgata aagaagaagt tggcagtcag ggaaatacag gtgctcaaag tatttttatt
      541 caggacttta tttccgattt actggctcat aataatgaag acaacagcag tgccaactta
      601 cgtaaaacct tcataaatag ccagatatta agcgctgatg taactgctgc cattgatccc
      661 aattatccta atgtgcacga aaagcaaaat gccgttattt tcaattatgg catcggcatt
      721 tccaaattta cgggaagagg aggaaaatat agttgcaacg atgccaatgc cgaatttatt
      781 gccaaagtta tccgcatttt caatcaggca ggagtttttt ggcaatcagg cgaactggga
      841 aaagtggatg aaggtggcgg cggaactatt gcctattttc tttccaattt gggagcggaa
      901 gttttagatt gcggagttgg tttaatggga atgcattcgc tgtatgaatt gtgttccaaa
      961 gcggatttat attctactta tcttggctat aaggcatttt tacaagccga ttaaagaatg
     1021 aaaaagtatt tgctgcccgt ctgctttctt ttactcattt caaaactcct ttcattccaa
     1081 gaaaccctaa cccttgatca aataataaca gaaatagcaa ctgaaattca gaccgggcag
     1141 cccatttttt tagacatcaa ttgcggagag tggaatcctg ttttaaccca aaaactgagt
     1201 accaatcttt tagaaaaagg tgcggacttg cggtataact tatctgaaac caaaacggat
     1261 atttcctccc tggaagaaaa aaaagtcccc gatctttcag cacttggatt ggaaaaagca
     1321 ttgctggttc agataaatct taatattgcg tggcaggaaa ccgtaaagag cagtttcttc
     1381 tcctaccgca ctgaacggta tcctgtccac atttttgaaa ttaggcagat tcaacttcct
//
LOCUS       DAOPFL010000231         1440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_130871_length_1440_cov_144.826715, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000231 DAOPFL010000000
VERSION     DAOPFL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130871_length_1440_cov_144.826715"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1440
                     /locus_tag="PLG69_08230"
     CDS             <1..>1440
                     /locus_tag="PLG69_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPI43531.1"
                     /translation="TIGLPARPAYTPWWAHTDNNHAWTEIYLDGEWHYTGDMDMAWFP
                     DNTWFSGLVDKTVLILADGTLTTWKDEVLIKGKYDAVINSTANYTKDRSRKVKIKVVD
                     SNNKPIPNANVIPMVFNWGSLRSLINLTTDKNGYLEFTTGRGAFYLSVYSKEGKALVL
                     IPSTAEKTIEKKITVTKTDFAGGWATLEYPENSVESKTQPEEYIIAVNLEKQRWQEKQ
                     KTLENEALKTKTTDDSLAIKVALACRGNYSAFWEFYKRNPNPDSNFLQFLLEGDPKLL
                     WQANAELLEALYNNWLKLYKPEMDEEKVMSLFSPVVYYENLPQVVNYKKGKAQLYPES
                     FRYNYPHNRDGVIKVLSRLKQKYKINSKKALSGLPPLHILVQHKNLTAVQYKIMAVCF
                     LRANGYPSEYSRIPNLIAVFIDGDWQYVNVVKCAWEDLSQDNKTNTFQLILQIKDDKG
                     NPIEAKEEQLTLCRYAEGAFYPLNDNFAY"
BASE COUNT          475 a          255 c          297 g          413 t
ORIGIN      
        1 gaacaattgg acttcctgcc cgtcctgcat atacaccttg gtgggctcat acggataata
       61 atcatgcctg gacggaaatt tatttggacg gagaatggca ttataccgga gatatggata
      121 tggcttggtt tcctgataat acctggttta gcggtctggt agataaaacg gtgctgattt
      181 tggctgacgg aactttaact acctggaaag atgaggtttt aattaaaggc aaatatgatg
      241 cggtaatcaa ttctacagct aactatacta aagaccgcag ccggaaagtg aaaatcaaag
      301 ttgtggatag taataataag ccaataccca atgccaatgt tattccaatg gtattcaact
      361 ggggttcctt aaggtcttta attaatttaa ctaccgataa aaacggctac ctggaattta
      421 ctacgggtag aggtgctttt tatctttcag tttacagcaa agaaggtaaa gctcttgttc
      481 tgattccttc cactgcggaa aaaactatag aaaagaaaat tactgttacg aaaactgatt
      541 ttgcaggtgg ctgggcaact ttggaatatc cggaaaacag cgttgaaagc aaaactcaac
      601 ccgaggaata tattattgcg gttaatctag aaaaacaacg ctggcaggaa aaacaaaaga
      661 cattggaaaa tgaagcgttg aaaacaaaaa ctacggatga ttccctggca ataaaagttg
      721 ctcttgcctg tagaggtaac tattccgctt tctgggaatt ttataagagg aatcccaatc
      781 ccgacagcaa ttttttgcag tttctgctgg aaggtgatcc caaactttta tggcaggcaa
      841 atgcagaatt gctggaagcc ctttataata attggctaaa actatataaa ccggaaatgg
      901 atgaagaaaa ggtgatgagt ttattctctc ccgttgttta ttacgaaaac ctgccacagg
      961 tggtaaacta taaaaaaggt aaagcacaac tctatccgga gtcattccgt tataattatc
     1021 cccataatcg ggacggagtt ataaaagtgc tttcccgctt gaaacaaaaa tataaaataa
     1081 actccaaaaa ggcattaagc ggacttcccc ctttgcatat tttagttcag cataagaact
     1141 taactgcagt gcagtataaa attatggcag tttgtttttt gagggctaat ggctatcctt
     1201 ctgaatattc gcgtattcca aatttgattg cagtgtttat tgatggtgac tggcaatatg
     1261 taaatgtggt aaaatgcgct tgggaagacc tttcccaaga taataaaacc aatacttttc
     1321 agcttatctt gcaaattaaa gatgataagg gaaatcctat tgaagctaaa gaagaacaac
     1381 ttaccctttg ccgctatgca gagggagcgt tttatccctt aaatgataat tttgcatatc
//
LOCUS       DAOPFL010000232         1436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_131453_length_1436_cov_74.194786, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000232 DAOPFL010000000
VERSION     DAOPFL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131453_length_1436_cov_74.194786"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            701..>1436
                     /locus_tag="PLG69_08235"
     CDS             701..>1436
                     /locus_tag="PLG69_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43532.1"
                     /translation="MIKSVVMQNFGPLVDTKWENLGKINVIIGANSSGKTFYLKALYT
                     AIKSLESYKRGNENRSLAEILADKLYWTFQVPKIGDIVTKGQPDPLSFEFSVGNKDLS
                     YTFGKDTTKKILSLENYIYPQKSDSIFLPAKEVLSIYQNIIQYYDQYKTFGFDYTYVD
                     LVKALSIPKIKGNAYKIITESRHKLREIVQGKIEFDTDSQVWQYKKGNVRYPIGVTAE
                     GIKKIGILDTLLGNRYLTPQSTVFIDE"
BASE COUNT          531 a          208 c          282 g          415 t
ORIGIN      
        1 gttacttaat cgtctatata acaagcaatt atgcattcta acacaataac gacaagaaat
       61 atggctaatt gttttattac caatgaataa ggcaatatta caaagaatta tacaggtagt
      121 gtctcaaaga ttggtgtaaa taaggtaacg cctgatgaag aaaatgttga gccggatccc
      181 gcactttgta ttgatgattt taacaaaact aaaagacaag gagaagatca taactcaacc
      241 gaatagaaag aaagatgaaa gagcggaagt agaatcaaat gcaccaactc actggagcgc
      301 ttaaatgaag agctcaagct cagagagaaa tgtttcagga tcttcctgga tgaaaagagt
      361 tgcctgcggc tgtttggtgc gatcctgcat agcttttcag aggattggat aagcggtaaa
      421 ttgtatcttt cggagctaat agaaagaatc agagaaaacc agaaaagggc tatatcattt
      481 gaggcaacgc agcaaggtag cttgttttga aaaagaactt gacttggatt tataagatga
      541 aaagaatgca attggtcgct tggcctaata tagattgtga gtaatgatga tagttagaat
      601 gtactcaaag aacctgagaa acaacgagtt ggaatttaca ccaacattta ggactcaact
      661 aaataaattc agtgttataa attaaattag gagataagct atgataaaat cagtagtaat
      721 gcaaaacttt ggtcctttgg ttgatactaa atgggaaaat ttgggaaaga tcaatgttat
      781 tattggagcc aattcctcag gtaagacatt ttatttaaaa gcattataca ctgctattaa
      841 gtccctggag agttacaaaa gaggtaatga gaacaggtct ttagcggaaa ttttggcaga
      901 taaattatac tggacttttc aagttcccaa aatcggggat attgttacaa aaggtcaacc
      961 cgatccgctt tcctttgaat tttcggttgg taataaagat ctcagctata cttttggtaa
     1021 ggataccact aagaaaatat tatcccttga aaactacata tatcctcaaa aaagtgattc
     1081 tatttttcta cctgctaagg aagttttatc tatctatcaa aatattatac aatattatga
     1141 ccaatacaaa acttttggtt ttgattatac ttatgttgat ttagttaaag ctctaagcat
     1201 tccaaagata aaagggaatg catataaaat tattacagaa tccagacata aactgagaga
     1261 aatagtccag ggaaaaatag agtttgatac tgattcccag gtatggcaat ataaaaaggg
     1321 gaatgttaga taccctattg gtgtaacggc tgagggcata aagaaaatag gcattttaga
     1381 tactttattg gggaatagat acttaacccc tcaatcaaca gtttttattg atgaac
//
LOCUS       DAOPFL010000233         1436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_131454_length_1436_cov_58.446054, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000233 DAOPFL010000000
VERSION     DAOPFL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131454_length_1436_cov_58.446054"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLG69_08240"
     CDS             <1..332
                     /locus_tag="PLG69_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPI43533.1"
                     /translation="AKRNYARYDYQKAKLQQRDTEELIALQIKQDYQKYYHAWENHNV
                     QTENIRLAERGLQLAQVRYENQVGIQLEVFDAQLTLQTIKLQYYQSIYEIISADRNFK
                     KSIGITL"
     gene            348..1373
                     /locus_tag="PLG69_08245"
     CDS             348..1373
                     /locus_tag="PLG69_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPI43534.1"
                     /translation="MKRYLIILTALILILISACGKKQEEGKSMEQIYSEEGVPVRQII
                     IEPTTFRQNLLYNATLNGMEETTVQAMVSEVITKINAKVGDRVAKDQIIVSFPQNTPS
                     AQYEQALTAFNSIKTTYERMQRLFAQGAISQQDLDNVETQYKVSKANLETSDKMINVR
                     APISGIITAIYVNPSEKVYPGKDLFTVASTNGYKATVMVPDTEINKIKKGTKATATWL
                     ETTISGRVTEIPLAMDNATKAFKVEVSFPGMNKKINYGVTAEISIEVFSKPNVIVVER
                     HQIVSENGTKYVWLNQDGKAVKREITTGLDNTLAFEITSGLNPGDLLITEGINMLTEG
                     AKLRVIE"
BASE COUNT          513 a          261 c          297 g          365 t
ORIGIN      
        1 cagccaaaag aaattatgcc cgctatgatt atcaaaaagc caaactgcaa caaagagata
       61 cagaagaact tattgctctg caaataaagc aggattatca aaaatattat cacgcttggg
      121 aaaatcataa tgtgcaaaca gaaaacattc gtttggcaga acgtggttta cagcttgccc
      181 aagtgcgtta tgaaaaccag gttggtattc agcttgaggt ctttgacgca caattaacct
      241 tacaaaccat taaactgcaa tattatcagt caatttatga aatcatatct gcagatagaa
      301 actttaaaaa atcaatcggt ataaccctat aacggagaag ataaactatg aaaagatact
      361 taattattct aacagccctg atactgatac ttatctctgc ctgcggtaaa aaacaggaag
      421 agggaaaaag tatggaacaa atatacagtg aagaaggggt tcccgttcgc caaattatta
      481 tagagcccac aaccttccgt caaaacctgc tttataatgc tactttaaac ggaatggaag
      541 agacaaccgt tcaggcaatg gtttcggaag tgattaccaa aatcaatgcc aaggtagggg
      601 atagggttgc aaaagaccag attattgtaa gttttcccca aaatacacct tcggcacaat
      661 atgaacaagc actaacggca tttaacagta tcaaaacgac ttatgaacga atgcaacggc
      721 tttttgccca aggtgctatc agccagcagg atttggataa tgtagaaacc cagtataagg
      781 tatctaaagc caatctggaa accagcgata aaatgatcaa tgtgcgtgct ccgataagtg
      841 gtattattac agcaatatat gtaaacccaa gcgagaaggt ttatccggga aaagacctgt
      901 ttacagttgc ctctactaat ggctataaag ctactgttat ggttcctgat acagaaataa
      961 acaagattaa aaaaggcact aaagcgactg ctacctggtt ggaaactacc atttccggaa
     1021 gagtaacgga gattcctctg gctatggata atgcaaccaa ggcatttaaa gtggaagtaa
     1081 gttttccggg gatgaataaa aagattaact acggagtaac agctgaaatt tccatagaag
     1141 ttttcagcaa acccaatgtg attgtggttg agcgtcatca aatcgttagt gaaaacggaa
     1201 ccaaatatgt ttggctgaat caggatggta aagccgtgaa aagagaaatt accacgggac
     1261 tggataatac gctggctttt gaaattacca gcggtttaaa tccgggtgat ttgcttatca
     1321 ctgaaggtat caatatgtta actgaaggcg caaaattgcg cgttattgag taagggaata
     1381 ggaactatgt ttttaacaga tctgtctatt aaccgaccag ttctggtcac aatggc
//
LOCUS       DAOPFL010000234         1426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_132838_length_1426_cov_79.771699, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000234 DAOPFL010000000
VERSION     DAOPFL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132838_length_1426_cov_79.771699"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1397)
                     /gene="wecB"
                     /locus_tag="PLG69_08250"
     CDS             complement(342..1397)
                     /gene="wecB"
                     /locus_tag="PLG69_08250"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPI43535.1"
                     /translation="MKIVQIVGARPQFIKLAPLSRKVRKHHQEVIIHSGQHYDIQMNE
                     VFFRDLEIPAPDYNLNVGSGSQSTQTAGILNALEPVLIKEKPDLTIVYGDTNTTLAGA
                     LTSAKLGIKTAHIEAGLRSYNKSMPEEINRIVADHISDILLAPTETAMHNASKEGLLE
                     KTFLVGDIMVDSLSFGIKKAEKEANVLSTLQLEDENYCLLTLHRPYNVDDPDNLYHIL
                     SGLDALQQLIIFPVHPRTLNILNNMKNNRFSNIRLIEPQAYLDFLLLMKHSRMIFTDS
                     GGIQKEAYILQKPCVTLRPETEWVETVQSGWNLLLPVFSRDFPFVVNNFKPPETHPAL
                     FGENVAEKIMQILERKG"
BASE COUNT          446 a          244 c          276 g          460 t
ORIGIN      
        1 taaacacgga gaaaaagttt tttaaagtag tagctcaaag agttgtaaga taaaattatg
       61 tagccggagg atgctgttcc tccgctataa gtgaaaagtt gaaaaagtgg aaaggtgaaa
      121 caaggtttgg acatcgtaaa aaaaggatgc gaagctacaa cgagctccgg ctacacaaaa
      181 agtcaggacg atttagcaag gcaaaatggg ttggaaaaat tccgatttgc caaagaaaca
      241 gaaggtttct aaatgaaatt ggggttttaa gacaaccctt ttttctattt accgacttgt
      301 ttttctttag ggtattgttt ggagcaatgc tgagataata tttatccttt tctttccaga
      361 atttgcatta ttttttccgc tacattttca ccgaagagag caggatgagt ttctggtggc
      421 ttaaaattat ttacaacgaa aggaaaatcc cgagaaaaaa cgggtaacag aagattccat
      481 ccgctttgga ctgtttctac ccattctgtt tcaggacgaa gagtaacaca gggcttttgt
      541 aaaatatatg cttctttttg aattcctccg gaatcggtaa aaatcatccg agaatgtttc
      601 atcagaagca gaaaatccag atatgcttga ggttctatca aacggatatt actaaaacgg
      661 ttgtttttca tattattaag aatattaagc gtgcggggat gaaccggaaa aattattagt
      721 tgttgtaagg catccaatcc tgagagaata tgatataaat tatcaggatc atctacatta
      781 taaggtctgt gcaatgtcag aagacagtag ttttcatcct ccaattgcaa agtagataag
      841 acatttgctt ctttttccgc tttttttatt ccgaaagaaa gtgaatcaac cattatatcc
      901 ccaaccagga atgttttttc cagcaaacct tctttgcttg cattgtgcat agctgtttct
      961 gtgggggcta ataaaatatc gcttatatga tcagctacaa tcctgtttat ctcttcgggc
     1021 atacttttat tataacttcg taatccggct tctatatgag ctgttttaat tcccagtttg
     1081 gcagaggtta aagctcctgc aagagtagta ttggtatcgc cataaacaat tgttaggtcg
     1141 ggtttttctt ttattaaaac gggctccaaa gcatttaata tacctgcagt ttgagtgctt
     1201 tggctgccgg aaccaacatt aagattataa tcaggagcag gaatttccaa atccctgaaa
     1261 aaaacctcat tcatttgaat atcgtaatgc tgtccactgt gaataattac ttcctgatgg
     1321 tgttttctaa ctttccggga aagaggagcc aattttataa attgaggtct ggctcctaca
     1381 atttgcacta ttttcatata taaatcctta tctatgtata actaat
//
LOCUS       DAOPFL010000235         1413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_134753_length_1413_cov_95.788660, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000235 DAOPFL010000000
VERSION     DAOPFL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134753_length_1413_cov_95.788660"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLG69_08255"
     CDS             <1..512
                     /locus_tag="PLG69_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPI43536.1"
                     /translation="ERFKVGISPDTNPAHFTIISGTNYIEAPVEWTEYTYQITQPGPL
                     YIGIQCVSNDAFFFCVDDVSIMGNDVNDPELPVVVTALNNNYPNPFNPETTISYSVKE
                     RGPVTIEIYNVKGQLVKTVVKGIQEPGNHTIVWNGTDDNGRSVSSGIYYYKMQAGKYS
                     STKKMIMMK"
     gene            complement(929..>1413)
                     /locus_tag="PLG69_08260"
     CDS             complement(929..>1413)
                     /locus_tag="PLG69_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43537.1"
                     /translation="SYASFVDNNPHNSVSVSVRNSTQDEIVSLYQVQFVLPNYFINKG
                     VPSGSILNLSKLNYVSGVDNLLAAYQLSVSTPTGEPINPDFYNIIGAQQEPYIYLPIT
                     EVATIKTAHLFYRDQLGNVTELNRVDNFSANYANEYVVVGNCFICTVPNPGIFYITKF
                     "
BASE COUNT          462 a          267 c          254 g          430 t
ORIGIN      
        1 tggaaagatt caaagtaggt atttcacctg ataccaatcc agcccacttc actataatca
       61 gtggaactaa ctatattgaa gcaccggtgg aatggactga atacacttat caaattaccc
      121 aaccaggacc tctttatatt ggtattcaat gcgtttccaa cgatgccttc ttcttctgtg
      181 tagatgatgt ttcaattatg ggaaatgatg ttaatgatcc tgaacttcct gtagttgtta
      241 ccgctttgaa caacaactat cctaacccct tcaatccgga aactactatt tcttacagtg
      301 ttaaagaaag aggacctgta actattgaaa tctataatgt taaaggtcaa ttagtgaaga
      361 ccgtagtgaa aggtattcag gaacccggaa atcatactat cgtctggaac ggaaccgatg
      421 ataatggtcg ttctgtttcc agtggtatct attactataa gatgcaagct ggaaaataca
      481 gcagcaccaa gaaaatgata atgatgaaat aacttctgca gctaaagctc ttccagcttt
      541 gcttggaagt aaatagtcaa attggaaacc cctgaacttt tacagctcgg gggttctttt
      601 ttatgttaga aattgatagc attttccccg aatgataatt cccagtccct atctaatttg
      661 gagaattatc tattatttcg gtgttttcgg tggctaaaaa atatatctgc gaaatctgca
      721 caaggtttat atactaatgt ttcgttgtta atcttttaaa gacacctcac atcctcttag
      781 gcgatcctct aacgatcctc taacacttcc ttaacgagat aagggaatcg ttagagaatt
      841 tttaagccag tataagaata agaaatagcc aaattaaata cacttttaac taataactac
      901 agctgactcc ccactcaggg aactaccttt agaatttggt gatataaaaa atccccggat
      961 tgggaacagt acagataaag caatttccta caacaacata ttcattcgca taattagcgc
     1021 taaaattatc cactcggttt agttctgtta catttcctaa ttgatcgcgg taaaacaaat
     1081 gagcagtttt aatagtagct acttctgtaa tagggagata aatatagggt tcttgttgtg
     1141 ctccgataat attgtaaaaa tctggattaa ttggctctcc cgtgggagtt gacacactaa
     1201 gctgataagc tgccaagaga ttatcaactc cagaaacata attcaatttg ctcaaattga
     1261 gaattgaccc tgaaggcaca cccttattaa taaaatagtt tggtagcaca aactgtacct
     1321 gatagagtga gacgatttca tcttgagtgg agtttctaac agaaacgctt acactattat
     1381 gagggttatt atctacaaag gaggcatagg aac
//
LOCUS       DAOPFL010000236         1411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_135074_length_1411_cov_53.792773, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000236 DAOPFL010000000
VERSION     DAOPFL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135074_length_1411_cov_53.792773"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..>1411)
                     /locus_tag="PLG69_08265"
     CDS             complement(9..>1411)
                     /locus_tag="PLG69_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPI43538.1"
                     /translation="DEVIILSSAITWEERDEREEMFSIALVLAQALSQHNFYKTLYSQ
                     ERLTTQKFEYIYGVSVPLNKILAECLHRNILQRYRSLDDLLNALINLPPVSECEIWTE
                     PEKAIPDNIEDRSKKDIPKTRIELPFWILIALIIVTVVMLLTTNIFSVLFGGKGEKLT
                     YSSWLMPNSESDTVNSSVPIAELPREKPTQTTYGELKTGTPTPREDFRKQPSVPKITT
                     VIPPPTAPKPRPPQNMVFIEASTLGFGRLRENPNPNVSLSSFYINKFEVTQEEWNIYM
                     KPANCSTFGENLPVDNISWFDIAIYCNGRSEAEGLTPAYKIRGVGASRVVTCDFKANG
                     YRLPTEAEWEMAAKAGKLYNYSGSDDPDEVSWNRDNSGGKIHTPGTKKPNDFGIYDMT
                     GNVAEWVWDWYDVNYIRSLPTFINPTGPETGTLKVIRGGSVMNGEGRNLNIMWREKGD
                     PNRGYQYVGFRLVRSN"
BASE COUNT          387 a          283 c          298 g          443 t
ORIGIN      
        1 aagaaatatt agttgctgcg cactaagcga aagcccacat attgataccc ccggttcggg
       61 tctccttttt ctctccacat aatgtttaga tttctacctt ctccattcat tacacttcca
      121 ccccggataa cttttaaggt tccggtttcg ggacctgtag gattgataaa agtaggcagg
      181 gaacggatat aatttacatc gtaccaatcc caaacccatt ctgcaacatt tcctgtcata
      241 tcgtaaatgc cgaaatcgtt gggttttttg gttcccggtg tatgaatttt accgccgctg
      301 ttatctctgt tccaagacac ctcatcgggg tcatccgatc cgctataatt atatagttta
      361 cccgctttag cggccatttc ccattcggct tcagtaggca gacggtagcc atttgcttta
      421 aaatcgcagg taacaactcg cgaagcgccc actcctctaa ttttatatgc tggggtcaaa
      481 ccctccgctt cacttctgcc attgcaataa atggcaatat caaaccagct gatgttatct
      541 acaggtaagt tttctccgaa ggtggaacag tttgccggtt tcatataaat attccattct
      601 tcttgtgtaa cttcaaattt attgatataa aaggaagata aggagacatt gggattggga
      661 ttttctctta agcgtccaaa acctaaagta cttgcttcaa taaaaaccat attttgagga
      721 ggacgaggtt tgggagctgt aggtggtgga ataacagtag ttattttggg aacagagggc
      781 tgcttacgaa agtcctctct gggggttgga gtgccggttt tcaattcacc ataagttgtt
      841 tgggttggtt tttcgcgggg caattcagct ataggaacag aactatttac agtatctgat
      901 tcgctattgg gcataagcca tgaggaataa gttaattttt ctcctttgcc accaaagaga
      961 acactgaaaa tattggtggt aagcaacatt acaacagtta cgatgatgag tgcaatcagt
     1021 atccaaaagg ggagttcaat tctggttttg ggaatatctt ttttgctacg gtcttctata
     1081 ttgtctggta ttgctttttc cggttcagtc caaatttcgc attcagaaac gggaggcagg
     1141 ttgattaagg cattcagtaa atcatccaaa ctgcgataac gctgtaaaat attgcgatgc
     1201 aaacattcag caaggatttt gttgaggggg acgcttactc cgtagatata ttcaaatttt
     1261 tgagtagtta agcgctcctg actgtataat gttttataga aattgtgttg ggataatgct
     1321 tgagctaaaa caagagcaat agagaacatt tcttcacgtt catctctttc ctcccaggtg
     1381 atagcggaag aaagaattat cacctcatca t
//
LOCUS       DAOPFL010000237         1399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_136843_length_1399_cov_111.587798, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000237 DAOPFL010000000
VERSION     DAOPFL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136843_length_1399_cov_111.587798"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PLG69_08270"
     CDS             <1..1293
                     /locus_tag="PLG69_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPI43539.1"
                     /translation="FHDYMDPVFWIHLNVDFPFNLKGILYFPKLRNEPDFFKGEVKLY
                     CNNVFVADNLEDLIPEFLLLLKGGIDIPDIPLNVSRSFLQNDAQVKKISQYIVKKVAD
                     HFNNTFKEDRKKYESYWEDINAFIKFGLLKDDDFFEAMKDIIIFKSASGDYVTIEEYK
                     ARNKSEDERTKIWYAASEDTQVSYLNLMKEQGIEVVFQTSPLDTHLYQQLENKLNNIE
                     FVRIDSEVNDLLLNKEGVASDSEDKLKELFYKALDQQVEASFSKESYADFLKKFPQAA
                     TLLSPYLIQKDEQTIIKPFELPENVRKELGAEAMKALEDNAFTEIKVEVKSLKSPEIP
                     SMIVFSEYMRRWHDMDLLAHQNPSSDMLKYHTLVVNRDNPIIKKILTFHEQGKDEEVK
                     TLCSYIHDLSLLEQKPFSGKELKSFIAKANQVLSYLSE"
BASE COUNT          475 a          249 c          279 g          396 t
ORIGIN      
        1 tttcacgatt atatggaccc tgtgttctgg attcatctga atgtggattt tcctttcaat
       61 ctgaagggga ttttatattt tcccaaattg cgcaatgagc ccgatttctt caaaggagaa
      121 gtgaaactct attgcaataa tgtttttgta gcggacaatt tggaagacct gattccggaa
      181 tttcttctgc tcttgaaagg cggaattgat attccggata ttcctctcaa tgtctctcgt
      241 agttttttac aaaacgatgc ccaggtgaaa aaaatcagcc agtatatagt aaaaaaagtg
      301 gctgaccatt tcaataatac cttcaaggaa gaccgcaaga aatatgaaag ctactgggaa
      361 gatatcaatg cttttatcaa attcggtttg ctgaaagatg acgatttctt tgaagcaatg
      421 aaggatatca ttatcttcaa atccgccagc ggcgactatg taacgattga agaatacaaa
      481 gcccgcaata aaagtgaaga tgaaaggaca aagatttggt atgcagccag tgaagatacc
      541 caagtcagct acctgaattt aatgaaagaa cagggtattg aagttgtttt tcaaaccagt
      601 cctctggata cccatcttta tcaacagctg gaaaataagc tgaataacat tgaatttgtg
      661 cgcattgata gcgaagttaa tgacctcttg ctaaataaag aaggggttgc aagtgactct
      721 gaagataagc tgaaagaact attttacaag gcattagacc aacaagtaga ggcaagtttc
      781 agcaaagaaa gttatgccga ttttctgaaa aagtttcctc aagctgcaac cctcttaagt
      841 ccttatctga ttcaaaaaga tgagcaaaca atcattaaac cctttgaact gccggaaaat
      901 gtgcgtaaag aactgggtgc tgaagcaatg aaagcccttg aagataatgc ttttaccgaa
      961 attaaagtag aagtgaagag cttaaaaagc cccgaaatac cttcaatgat tgtgtttagt
     1021 gaatatatgc gtcgctggca tgatatggat ctgctggctc atcaaaatcc ttcttccgat
     1081 atgcttaaat accatacttt ggtggtaaac agggataatc ccataatcaa aaagattctt
     1141 acctttcacg aacagggtaa ggacgaagaa gttaaaaccc tttgctccta cattcacgat
     1201 ctttcgcttt tggagcagaa acctttcagc ggcaaagaat taaagagttt tatcgctaaa
     1261 gcaaatcagg tgcttagcta cttaagcgaa taaataaaaa taattgcagg gttttttaat
     1321 cccgaaagta taaactacag ggactgtata atgcagtccc tgtaattttt ttgcctgcaa
     1381 agaggtgaat atgaaaaaa
//
LOCUS       DAOPFL010000238         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_137608_length_1394_cov_82.174010, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000238 DAOPFL010000000
VERSION     DAOPFL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137608_length_1394_cov_82.174010"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PLG69_08275"
     CDS             complement(<1..139)
                     /locus_tag="PLG69_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPI43540.1"
                     /translation="MKSLLKEDLYAKIFHSALLAIAVTDKQGNFIAVNPAWCKALGWT
                     EE"
     gene            complement(208..>1394)
                     /locus_tag="PLG69_08280"
     CDS             complement(208..>1394)
                     /locus_tag="PLG69_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPI43541.1"
                     /translation="KLRENVYWVGVIDWDLRNFHGYITQRGSTYNAYLIIDEKVTLID
                     NVKSYLFEEMLSRISDVIEPSKIDVLIQNHTEMDHSGGLPELIKIIPDAELYTNANGI
                     KAQKRHFKQDWNFREIKSGDTLKIGKRTLTFLTTPMVHWPDNQFTYCPEEKILFSNDG
                     FGQHIASNERFAKELPAGIVMEEAKKYYANIVLPYSKQVQKVLEEAGKWDINMICPSH
                     GVIWTAENIPEILSAYHNWAYNIADDYKALIIYDTMWKSTQKLAYTISAAFEAEGIRA
                     QLVNLQTNHISDIMTAVMDAKFIAVGSPTLNSSILPTVAAFLYYLKGLSPRNKIGLAF
                     GSYGWGGQSIPILHQLLGDPKECGFAMLEPVQVQYIPEEEELNRIKSNLKRQIREKIN
                     AM"
BASE COUNT          426 a          285 c          228 g          455 t
ORIGIN      
        1 cttcttccgt ccaacccagg gctttacacc aggcaggatt tacggcaata aaattacctt
       61 gcttatcggt taccgcaata gcaagtaagg cactatgaaa aatcttggca tacaggtctt
      121 cttttaataa agatttcata tccgcttcct ttattttgaa tatggttaat taaacagtta
      181 caggcagata aatacgctct gatgttatta cattgcattg attttttccc ttatttgccg
      241 tttcaaatta ctttttatcc ggttaagttc ttcttcctca ggaatatatt gcacttgaac
      301 cggttccagc attgcaaaac cgcattcttt agggtctcct aaaagctgat gtaaaattgg
      361 tatactttgt ccaccccaac cgtaacttcc gaaagcaaga ccaatcttat tccggggtga
      421 aagtcctttt aaataataaa gaaaggcagc taccgtaggc aaaatggaac tgtttaaagt
      481 tggtgagccg acagcaataa atttggcatc catcacggca gtcataatat ctgaaatatg
      541 attggtctgc aaattaacta attgagctct aatcccttct gcttcaaatg cggcagaaat
      601 agtataagca agtttttgag tggatttcca catcgtatcg taaataatta aagctttata
      661 atcatctgca atattataag cccaattgtg gtaagcagaa aggatttcag gaatattttc
      721 cgctgtccag ataactccat gactgggaca aatcatattt atatcccatt ttccggcttc
      781 ttccaaaacc ttctgaacct gtttggaata gggcaaaaca atattggcat aatacttttt
      841 tgcctcttcc ataactatgc ctgctggaag ttctttggca aagcgttcat tagaagcaat
      901 atgctgacca aaaccatcgt tggaaaacag gattttctct tcaggacaat aagtaaattg
      961 attatcaggc caatgcacca tcggagtagt taaaaaagtt aaagtccttt tcccgatttt
     1021 gagggtatct ccgcttttga tttctctaaa attccagtcc tgcttaaaat gccttttctg
     1081 agcttttatt ccgttagcat tagtgtataa ttcagcatcg ggaattattt taattagttc
     1141 cggtaagcca ccgctgtgat ccatttctgt atgattttga attaaaacat caatcttgga
     1201 gggctcaatt acatcactta tccgggaaag catttcctca aacagataac tctttacatt
     1261 atcaataaga gtaacctttt catctataat caaataggca ttataagtgc tgccccgttg
     1321 agttatatat ccatgaaaat tacgcaaatc ccaatcaata actccaaccc aatatacatt
     1381 ttctcttaat ttga
//
LOCUS       DAOPFL010000239         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_138158_length_1390_cov_102.079401, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000239 DAOPFL010000000
VERSION     DAOPFL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138158_length_1390_cov_102.079401"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLG69_08285"
     CDS             complement(<1..266)
                     /locus_tag="PLG69_08285"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPI43542.1"
                     /translation="MSRANESALQQEIMAWIKQNQYVYLATVDKNRPRVRPVVLFIYD
                     DRFFVTTFSGDSKVSQIQNNKNIEVCLPLYEDGQTGYIRLSGTAS"
     gene            complement(279..1139)
                     /locus_tag="PLG69_08290"
     CDS             complement(279..1139)
                     /locus_tag="PLG69_08290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPI43543.1"
                     /translation="MRHLNKNIIPILVVLMMITISVGCQTKTPSGEKPAEQLMKEKEI
                     ITNKTKVEMETNYGTIELELWDDLTPKTVQNFVKLARSGYYDGLYFHRVIPDFMIQGG
                     CPNTRDKDRSNDGRGGPGYTFEDECYRNGEEITGKIDTNQKAEAVWNKIIVPHIQNNR
                     TPNAEIAAIAKECQSTHSLEPVMKYDLKFYQEKTGIMDKVYVQELIAPVDYGTICMAN
                     SGPNTNGSQFFIVTKKDGAHCLDGQHTVFGRVTKGMEVAHKIENLPRDKNDNPNPENQ
                     AIITRISFPK"
     gene            complement(1156..>1390)
                     /locus_tag="PLG69_08295"
     CDS             complement(1156..>1390)
                     /locus_tag="PLG69_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine hydrolase"
                     /protein_id="HPI43544.1"
                     /translation="QELVDKKSKGLKNYAKWLIEHKKADIVVLGHSHQPVWETMGNGS
                     YLNCGDWITYYSYVEIDAGKPALKEYNIKNEQI"
BASE COUNT          359 a          283 c          265 g          483 t
ORIGIN      
        1 gaggcagttc cacttaagcg aatatagccg gtttggccat cttcgtaaag cggaaggcag
       61 acctctatat ttttattgtt ttgaatttgg ctcaccttgg aatctccgct gaaggtagta
      121 acaaaaaagc ggtcatcgta gataaatagg acaacgggac gaactctggg tcggttttta
      181 tctacagtag ccagataaac atactgattt tgttttatcc aagccataat ttcttgttgc
      241 aaagcggatt cgttggcgcg gctcatttta gttctttatt atttgggaaa agaaatacgg
      301 gtaataattg cctgattttc ggggttgggg ttgtcgtttt tatcgcgagg caagttctca
      361 attttatgag caacttccat acctttagta actctgccga aaacggtatg ctgaccatcc
      421 agacagtgag ctccatcttt tttagtgacg ataaagaact gactgccatt agtattggga
      481 cctgaatttg ccatacaaat cgtcccatag tccaccggag caattaattc ctgcacatag
      541 accttatcca taattccggt tttttcctga tagaacttga gatcatattt cattacgggc
      601 tctaaactat gtgttgattg acattcttta gcaatagcag caatttccgc atttggagtg
      661 cgattatttt gaatgtgtgg cacaataatt ttattccaga cagcttcagc tttctgattg
      721 gtatctattt taccggtaat ttcttctcca ttcctataac attcatcttc aaaagtataa
      781 cctggaccgc cgcgtccatc attgctgcgg tctttatctc tcgtattggg gcatccaccc
      841 tgaatcataa aatccggaat gacgcgatgg aagtataagc cgtcgtaata tccggatctg
      901 gcaagtttta caaaattctg aacggttttg ggagtaagat catcccaaag ttccagttca
      961 atggtgccat aatttgtttc catttcaact tttgttttgt tagtaataat ttctttctct
     1021 ttcatcaatt gctccgccgg tttttctccg gaaggtgttt ttgtttggca tccgacactg
     1081 attgtaatca tcattaaaac aaccaggatc gggataatgt ttttgtttaa gtgcctcata
     1141 atatactcct ttgtgttata tttgttcatt ttttatatta tactctttca gagctggttt
     1201 tcctgcatca atttccacat aagaataata ggttatccag tcaccgcaat taaggtaaga
     1261 gccgtttccc atagtttccc aaacgggctg gtgactatgt cccaaaacca caatatcggc
     1321 ttttttatgt tcaatcaacc atttggcgta attttttaag cccttgcttt ttttgtcaac
     1381 aagttcctgg
//
LOCUS       DAOPFL010000240         1358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_143257_length_1358_cov_135.287030, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000240 DAOPFL010000000
VERSION     DAOPFL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143257_length_1358_cov_135.287030"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="hflX"
                     /locus_tag="PLG69_08300"
     CDS             complement(<1..767)
                     /gene="hflX"
                     /locus_tag="PLG69_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPI43545.1"
                     /translation="MNNEEYKALDEFELEPENWEQAERTEKTAFLAALANSRETDKDI
                     KESLNELERLAETAGIQVLGTYHQRRNAPERGTYFGKGFLTDLSRKMMQAQADILIVN
                     DELGPSQAYNIERDYQIKVMDRTEVILDIFHKHAKTREAKLQVHLAELEYQLPRLKRM
                     WEHFDKERGGVRNTGGTATRGMGEKQIEIDRRLIKDKIRKINKAIETIQHQKETQRKQ
                     REKAKKICLVGYTNAGKSTLFNQLTNAGVLVEDKLFAT"
     gene            969..1054
                     /locus_tag="PLG69_08305"
     tRNA            969..1054
                     /locus_tag="PLG69_08305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1003..1005,aa:Ser,seq:tga)
     gene            1080..1171
                     /locus_tag="PLG69_08310"
     tRNA            1080..1171
                     /locus_tag="PLG69_08310"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1115..1117,aa:Ser,seq:gct)
     gene            1189..1263
                     /locus_tag="PLG69_08315"
     tRNA            1189..1263
                     /locus_tag="PLG69_08315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1221..1223,aa:Gln,seq:ttg)
     gene            1279..1352
                     /locus_tag="PLG69_08320"
     tRNA            1279..1352
                     /locus_tag="PLG69_08320"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1311..1313,aa:Gln,seq:ctg)
BASE COUNT          339 a          300 c          272 g          447 t
ORIGIN      
        1 gtggcaaaaa gtttatcttc aaccagcaca ccggcattag ttaactgatt aaaaagtgtt
       61 gatttaccgg catttgtata gcccactaaa caaatctttt ttgctttttc gcgttgcttg
      121 cgttgtgttt ctttttggtg ctggatggtt tctattgctt tgttgatttt tcggatttta
      181 tctttaatca ggcgtctgtc tatttcaatt tgtttttcac ccattcctct tgtagcagtg
      241 ccgccagtat ttctcactcc accacgttct ttatcaaaat gttcccacat tctttttaag
      301 cgaggcaatt gatattccag ttcagcaaga tgaacttgta gtttggcttc ccgcgttttg
      361 gcatgtttat gaaagatatc taaaatgacc tcggtgcggt ccataacctt tatttgatag
      421 tccctttcaa tattatatgc ctgagaagga cctagttcat cattaacgat taggatgtct
      481 gcctgtgcct gcatcatttt tcggcttaaa tcagtcaaaa accctttgcc aaaataggtt
      541 cctctttcgg gagcatttct tctttgatgg taagttccca aaacctgaat tccagccgtt
      601 tcagccaggc gttccagttc gtttaacgat tccttgatgt ctttgtctgt ttctcttgaa
      661 tttgccaaag cagctaaaaa agccgttttt tcagttcttt cggcttgttc ccaattttcg
      721 ggctccagct caaattcatc cagtgcctta tattcttcat tattcatatt ttccttttat
      781 cctgtgctta tttttgctgt caaggaaaaa gcagtggtct gtcaaattcc tctttggcag
      841 aaaggtgata aaaaataata gacactcttc tccctttccg taaagataca attttcaaac
      901 tttcttgaac tggcaagaaa gggcttgaca gaaacccctt ataaaaattt caaggataaa
      961 atatagaggg aggattagtc ctaattggta aggcagcggt cttgaaaacc gccgggttat
     1021 gcccttgggg gttcgaatcc ctcatcctcc gccagaaaaa taaggcagaa aaaataacgg
     1081 gagaggtgac cgagttggct taaggtgcac gcctgctaag cgtgtgtgga ttaaaagtcc
     1141 acccagggtt caaatccctg cctctccgcc acttataaaa aaaattgctg ggcagtcgcc
     1201 aagtggtaag gcagcaggtt ttggtcctgc catccgaggg ttcgaatcct tcctgcccag
     1261 ccaaaatata aaacgcattg ggatgtcgtc taatggtagg acagcggact ctggatccgt
     1321 atgtgagggt tcaactcctt ccatcccagc cagtttta
//
LOCUS       DAOPFL010000241         1358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_143258_length_1358_cov_98.778204, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000241 DAOPFL010000000
VERSION     DAOPFL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143258_length_1358_cov_98.778204"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLG69_08325"
     CDS             complement(<1..1027)
                     /locus_tag="PLG69_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43546.1"
                     /translation="MPKQNTCNKLLLGIIALIFALNLKAAMVNNLPVTVFQPDGTKLE
                     LLASGDEYHNWLHDKNNYTIIRHPESGYLCYAEQDRENVKASNLIVGKDDPFRSALKP
                     GINISEEAYKELRSSKFWMPAERDAPTTGTINNIVIFIRFSGESEFGQGISVYDGWFN
                     SGTNSQKNYFLEASYNQLTVNTTFYPPAQNNLVVSWQDSHPRAYYEPYNATTNPTGYN
                     GDDERRNREFTLLQNAVAGVAAQVPSSLNIDSDGDGKVDNVVFLVSGAAGEWSSLLWP
                     HRWAIYDRNVYLNNKRVYDFNLQLKDFLATRGVGVICHEFFHTLGAPDLYHYTSNGID
                     PAGSWDLM"
BASE COUNT          422 a          283 c          239 g          414 t
ORIGIN      
        1 acattaaatc ccagctaccg gcaggatcaa ttccattact ggtataatga taaaggtcgg
       61 gagcaccaag agtatgaaag aattcatgac aaataacccc tactccgcgt gtggcaagaa
      121 aatccttcag ctgaaggtta aaatcgtaaa cgcgtttatt gtttaaatat acattgcgat
      181 cataaattgc ccatcggtgg ggccaaagca aactactcca ttcaccagcc gctccactaa
      241 cgagaaaaac aacattatcc actttgccat ctccatcgga atcaatattt aaagaagaag
      301 gaacctgagc agcaactccg gcaacagcat tttgaagtaa agtaaattcc cggtttctcc
      361 tctcgtcatc tccattataa cctgtcggat tagtagtggc attatagggt tcataataag
      421 ctctcggatg gctatcctgc caggaaacaa ccagattatt ttgtgccggc ggataaaaag
      481 tagtatttac cgtaagctga ttatagcttg cttccagaaa ataattcttt tgagaattgg
      541 ttccgctatt gaaccaaccg tcataaacgc taataccctg gccaaattca ctttctccag
      601 aaaaacggat aaagatcaca atattattaa ttgtaccggt ggttggtgca tctctttctg
      661 ccggcatcca aaacttgcta ctgcgtaatt ccttatatgc ttcttcagag atgttaattc
      721 ctggcttaag agcagaacgg aaaggatcat ctttacccac gattaaattg cttgctttaa
      781 cattctctct atcttgttcg gcataacata agtagccact ttcgggatgc ctgataatag
      841 tgtaattgtt tttatcgtgc agccagttat gatattcatc gccacttgcc aataattcca
      901 gttttgttcc atccggttga aaaacggtaa ccggcaggtt attaaccata gcggctttta
      961 aatttaaggc aaatataaga gctattatcc caagtaacag cttgttacaa gtattttgct
     1021 tcggcatttc gtctcctttt gggtatgctt gcttttagaa aaaacttaat tacattcaac
     1081 cataaatttg tcaatcagaa ccttgtaaac acaaataatg taattttcaa tttggtaagt
     1141 cctaaaaaac ccgttaagaa tagcagtttt atccccaata atgcagtaga tgaagaatgg
     1201 gtaggcaaat tctgatttgc ccaagagttt gcaacactct gaaaaataaa ttgtttggtt
     1261 tatggtaaat aagaatgctg ccaaccaaat accaattttg actttttatt cagggttgaa
     1321 acccttttct aatatctgtc gttcctacgg aactcttt
//
LOCUS       DAOPFL010000242         1354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_143893_length_1354_cov_136.344881, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000242 DAOPFL010000000
VERSION     DAOPFL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143893_length_1354_cov_136.344881"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLG69_08330"
     CDS             complement(<1..263)
                     /locus_tag="PLG69_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPI43547.1"
                     /translation="MTKADLVKIISENTGIIRKDVAVVVDALLYAIKDSMAKGNHIEI
                     RGFGTFKLKTRKPRVGRNPKTDQKVPVPARTVPTFKFSREFKNA"
     gene            718..1353
                     /locus_tag="PLG69_08335"
     CDS             718..1353
                     /locus_tag="PLG69_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43548.1"
                     /translation="MQTAVQDSVPDVYSFRCEGKKIILQHSEKKYILYYSKGLNYILT
                     LLEYPELNIPYSNLESNLSSPEKAYSQFSSPQEICEQQLKVQDGFLAVPMTDLQTIKE
                     VKEELIQVINELAELEENCDYARADELRDIYEKLSKYLRDVYHKDCKIRHFTCEESKI
                     KRRVVRAVNRALEEIEIIEPELAKELRKSIEFGKTICYHQRRKIEVFGYWK"
BASE COUNT          433 a          216 c          275 g          430 t
ORIGIN      
        1 gcgtttttga attcgcggga aaatttgaag gtaggaacgg ttctggcagg aacaggaact
       61 ttttggtctg ttttggggtt acggccaacg cgaggtttac gggttttgag tttaaaggtt
      121 ccaaaaccac gaatttcaat atgattgccc ttagccatac tatctttaat ggcatacaaa
      181 agggcatcaa caacaacggc tacatctttg cgaatgattc cggtgttttc cgaaataatt
      241 tttactaaat cggctttcgt catttttctt tctccttaaa ttagtttttt tcttgctgtt
      301 cggcaataag gtattcatag ataaaacctt aggatttatg tcaagggaaa aatctgatgc
      361 tgaagctgaa aaattgttca aatccccttt ataataggga aaaacgggtg tttcgtgggc
      421 ataaatattt tatctttttg taccttattc ctgtaactta acctattcca acgagattga
      481 aaagcactat gatgttaagt tcgcaaattc cgattgggtg aagacagatg tctattgatt
      541 ttgtggctat tttaagcacc caagttctat attcgttagt tgtaagataa ttaaaccccg
      601 gagagaaaaa gattagcaat actaattttc tgcttgacaa tgttagcaat gctaatttta
      661 tggctctatg ctatgcacaa caaaatgtaa tatttaatac acaaaagaag gtgaaatatg
      721 cagacggcag ttcaagattc cgttcccgat gtttattcct ttcggtgtga aggtaagaaa
      781 attatccttc agcattctga aaagaagtat attctttatt acagcaaggg tttgaattat
      841 attcttactt tactggaata tccggaattg aatatcccct attccaattt ggaatctaat
      901 ctttcttccc cggaaaaagc atacagccag ttttcttctc cccaagaaat ttgtgaacag
      961 caacttaagg ttcaggatgg ttttcttgcc gttccaatga ctgatttgca aacgatcaaa
     1021 gaagtgaaag aggaattgat tcaagtgatt aacgagcttg ccgaactgga agaaaattgc
     1081 gattatgccc gggctgatga actgcgggat atttatgaaa aactgagtaa gtatttgcgg
     1141 gatgtttatc ataaagattg taaaatcaga cacttcactt gtgaagaaag caaaattaaa
     1201 agaagagttg ttcgcgctgt taatcgtgct ttggaagaaa ttgaaattat agaacccgaa
     1261 ctggcaaagg aattaagaaa atcaatagaa ttcggaaaaa cgatttgcta tcatcaaagg
     1321 aggaaaattg aggttttcgg atattggaag taga
//
LOCUS       DAOPFL010000243         1329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_148165_length_1329_cov_126.781005, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000243 DAOPFL010000000
VERSION     DAOPFL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148165_length_1329_cov_126.781005"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="yidC"
                     /locus_tag="PLG69_08340"
     CDS             complement(<1..798)
                     /gene="yidC"
                     /locus_tag="PLG69_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPI43549.1"
                     /translation="MEKRTLLALLLVFILFLVFNQFVWKPQQLRQQQEQQNQQTQTPQ
                     TVTEPVIPDTIIITPMPDTLLAKEKTPEIIKLSNQLMTVTFNSRGGSIQQVELHNFIM
                     HDSTKVKLIPENSSLADVKLIHPAAETPLKDVIFQYKISPDSLGVTFFLGDETNPQIS
                     KSFALDNQYGISFAINVENYQVINGIEVDFSCGIADTETVTKSKQQDYRFMYYADNEI
                     FKTTLAKMKKKQPSGNLNSFNWMALRGKYFTLVLKENEPALMRSFRTE"
     gene            complement(809..1039)
                     /gene="yidD"
                     /locus_tag="PLG69_08345"
     CDS             complement(809..1039)
                     /gene="yidD"
                     /locus_tag="PLG69_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPI43550.1"
                     /translation="MRQILHFPNLLFLTLIRFYQLAVSPFLPASCRFEPSCSVYSYEA
                     FKKYNFFKAFYLTAWRILRCNPFHKGGYDPLP"
     gene            complement(1036..>1329)
                     /gene="rnpA"
                     /locus_tag="PLG69_08350"
     CDS             complement(1036..>1329)
                     /gene="rnpA"
                     /locus_tag="PLG69_08350"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPI43551.1"
                     /translation="FFKPDFMIRSSYFYVPGLYREGEIAVGITISNKVGKAVKRNLLK
                     RRIKSWLRTNQDKIPVGYRFNLIARSGATELEWQELSRELTNIIAQLNIQTRS"
BASE COUNT          396 a          235 c          262 g          436 t
ORIGIN      
        1 ttctgttcta aaggaacgca ttaaagcggg ctcattttct tttaagacga gagtaaaata
       61 ttttcctctc aaagccatcc aattgaaact gttaagattt ccagatggct gttttttctt
      121 cattttggcg agagttgtct taaaaatttc gttatcggca taatacataa agcggtaatc
      181 ctgctgtttg cttttggtta cagtttccgt atccgcaata ccacaggaaa aatcaacttc
      241 aataccatta ataacttgat aattttccac atttatagcg aaagatatgc catattgatt
      301 atccaaggca aagcttttac taatttgggg atttgtttca tcacccagga aaaaagttac
      361 tcctaaagaa tcgggagaaa ttttatattg aaaaataaca tcttttaacg gtgtttcagc
      421 tgcgggatga atcagtttta catctgccag agaactattt tcgggaatta gtttcacttt
      481 tgtgctgtcg tgcattataa aattatgcaa ttccacttgt tgaatagaac ctccgcggct
      541 gttgaaggta accgtcatca actgattgga gagttttatt atttctggtg ttttttcctt
      601 tgccaaaaga gtatccggca ttggagtaat gataatggta tccggtatta ccggttccgt
      661 aactgtttga ggagtttggg tttgttgatt ttgctgttcc tgctgttgac gcaattgttg
      721 aggtttccaa acaaattgat tgaaaacaag gaaaaggatg aacaccagga gtaaggcaag
      781 taaggttcgt ttttccattt attgtccttt agggaagtgg gtcgtaaccg cccttgtgaa
      841 aaggattaca acgcaaaata cgccaggcgg ttaaataaaa tgccttaaaa aaattgtatt
      901 ttttaaatgc ttcataactg taaacactac agcttggttc aaatctgcaa ctggctggta
      961 aaaaaggaga tacggcaagc tgataaaacc ggatgagggt taaaaaaagc agattcggaa
     1021 aatgtaaaat ctgtctcaag accttgtttg tatgttcagc tgtgctatta tgtttgttaa
     1081 ttctcgggat aattcctgcc attctaattc ggtagctcca cttctggcta tcagattaaa
     1141 ccggtaacca acaggtattt tgtcctgatt ggtgcgcaac caggatttga ttctgcgttt
     1201 aagtaaattt cttttaacag ctttgcccac cttattgctg atagtgatgc ccactgcaat
     1261 ttcaccttct ctgtataatc caggcacata aaaataggaa gacctgatca taaaatccgg
     1321 cttaaaaaa
//
LOCUS       DAOPFL010000244         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_149383_length_1322_cov_53.907656, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000244 DAOPFL010000000
VERSION     DAOPFL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149383_length_1322_cov_53.907656"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1322)
                     /locus_tag="PLG69_08355"
     CDS             complement(<1..>1322)
                     /locus_tag="PLG69_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43552.1"
                     /translation="ETADKYLKSGSAEAELIILQYIQQDRVSEDDEEWVYNLLEKASN
                     PYIKLNALLWLSAKGKYLTQLSKLWGISVNELKSLSQQQPKIGLFPAVDSQKNAFLAK
                     VFVYKLKSEEPIALAILGDKIENFGYLAQLGKQNCLIGFNKNIQGNSWQLAVLATLLA
                     KDENQMAKIAYSGIVLPSGEIITAEEIEYKKRCCQNLVHRIKKIEQLDAWLNTETIPL
                     PVIQYQGEENELKRWQKAMEQKVQEKFSWFSYELLEDFYGITNSDLAIFGNGILPFEA
                     NAWQKLLQEQVKDKFKLLEDKVMPKKVLWFYAGQISTLQLGIGALFGFKRAVSILQME
                     FSNTTYHEVFTLYGKENARQLKNVSVKKEDYQYIQSELLINEPHKNELGFIIYLGSHN
                     PIGEAKAYCKKQLQINNFLIIQARENQGVMETSQNWLPYVQEINSALN"
BASE COUNT          355 a          261 c          217 g          489 t
ORIGIN      
        1 atttaaggca gaatttattt cttgcacata gggcagccag ttttggcttg tttccattac
       61 tccctgattt tctctggctt gtataattaa gaaattattt atctgtaatt gttttttaca
      121 atatgctttg gcttctccaa tcggattatg gcttcccaga taaataatga aacccagttc
      181 gtttttatgt ggctcattaa ttaatagttc gctttggata tattggtagt cctctttttt
      241 aacagaaaca ttttttagtt gcctggcatt ttcttttccg tagagggtaa aaacctcgtg
      301 gtaagtagta ttggaaaatt ccatttgtaa aatactaact gctcttttaa atccgaaaag
      361 agcaccaatt cccaattgca aagtgcttat ttgtccggca taaaaccata agaccttctt
      421 gggcatcacc ttatcttcca gaagtttgaa tttatctttc acctgttctt gcagcaattt
      481 ttgccatgca ttggcttcaa aaggaagaat gccattaccg aaaatagcca ggtcactatt
      541 tgtgataccg taaaaatctt ccaatagttc atagctaaac cagctgaatt tttcctgcac
      601 tttctgttcc attgcttttt gccaacgctt tagttcattc tcttcacctt gatattgaat
      661 aacaggcagg ggaatggttt cagtatttaa ccaggcatcc agctgttcaa ttttttttat
      721 tctatgcacc agattttggc agcagcgttt tttgtattca atttcttctg ctgtaataat
      781 ttctcctgaa ggcaaaacaa taccgctata ggcaatttta gccatctgat tttcatcttt
      841 tgccagcaaa gttgccaaaa ctgccagttg ccaggaattt ccctgtatat ttttattaaa
      901 accgataagg cagttttgct ttcctaattg tgccaaatag ccgaagtttt caattttatc
      961 gcctaaaatg gctaatgcta tcggttcctc ggatttcagt ttatatacaa atacttttgc
     1021 taaaaaagca tttttctggc tatctaccgc tgggaaaaga cctattttag gttgttgctg
     1081 gctaagggat tttagttcgt ttaccgaaat tccccaaagc ttacttaact gggttaaata
     1141 ttttccctta gcggaaagcc aaagcagagc attcaatttt atataaggat tgcttgcttt
     1201 ttccagtaaa ttataaaccc actcttcatc atcttctgaa actctatctt gctgaatgta
     1261 ttgcagaatt ataagttctg cttctgccga accgcttttt aaatatttat cggctgtttc
     1321 gt
//
LOCUS       DAOPFL010000245         1320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_149739_length_1320_cov_93.738340, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000245 DAOPFL010000000
VERSION     DAOPFL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149739_length_1320_cov_93.738340"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>1320)
                     /locus_tag="PLG69_08360"
     CDS             complement(11..>1320)
                     /locus_tag="PLG69_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPI43553.1"
                     /translation="QTYNLTGIFNLEWDITSCEAVLFLQNNATKEILQGVKYSLPELL
                     GINPISVTELNFPETYIGGSSSLSFTINNYFNFTVTGTISSSNPVFIPEQTNFSIAAY
                     QATTINVNFVPASAVQYTGILTLNSNLSGFNIVQIPLSGTGFFNAPPSVSNLIIVGPP
                     VIYQGLTVLYDFSDPDGNVEGNSRFQWMRMFNNQPQVIQNAVQQTYIIQPEDMDYPIA
                     CQVTPVDQHGMEGTPVLSPPTIPIEDLPAPQNLTGTLTPPNTVILHWQKPIHYQGKVF
                     SGYRIYRDGTSLYSLMNPDSLSYVDLNVPDGSHTYWACSIFTYPLIFSNPSNSITINV
                     NVPNSDELAPALKSVSVYPNPFKATTNFQISGKANRPVTIEIYNLKGQLINRLTTKTD
                     QNGSAQIGWNALDESAKPVRNGIYFYRTLCENFCETGKLILLK"
BASE COUNT          427 a          222 c          283 g          388 t
ORIGIN      
        1 tgtttactga ttactttagc aggataagtt ttccggtttc acagaagttt tcacagaggg
       61 tgcgatagaa atatattccg tttctgacgg gtttggcact ttcatctaaa gcattccagc
      121 cgatttgagc tgagccgttt tggtctgttt tggttgtcaa acggtttatc agctgtccct
      181 tcaaattata gatttcaata gtaacaggac gattggcttt accggaaatt tggaaattag
      241 ttgttgcttt gaaaggattg gggtaaacac taacgctttt caaagccgga gcaagctcat
      301 cactattagg cacattcaca ttaatcgtaa tggaattgga aggattggaa aaaatcaggg
      361 gataagtaaa aattgaacat gcccaataag tatgcgagcc atcaggaaca tttagatcaa
      421 catacgaaag tgaatcggga ttcattaaac tgtataaaga tgttccatcg cggtatatac
      481 gatatccgct gaagaccttt ccctgataat gaatgggttt ttgccaatgc aggataacag
      541 tattgggagg agttaaagtt cctgtaagat tttgaggtgc aggcaaatct tcaataggaa
      601 ttgtaggagg acttaaaaca ggagttcctt ccataccgtg ttggtcaaca ggagttacct
      661 gacaggcaat tggataatcc atatcttcag gttgaataat ataggtttgt tgaacagcat
      721 tttgaatcac ctggggctga ttattgaaca ttctcatcca ttggaacctg ctattgcctt
      781 caacattgcc atcgggatca gaaaaatcat aaagaaccgt aagcccctgg taaattaccg
      841 gtggacctac tattatcaaa ttgctgacag aaggaggcgc attaaaaaaa ccggttccgg
      901 aaaggggaat ttgcactata ttgaaaccac tcagattgct gttgagtgtt aaaattcccg
      961 tatattgaac agcagaagca ggaacaaaat ttacatttat agtggtagct tgataagccg
     1021 caatactaaa attggtttgt tcaggaataa aaacaggatt ggatgaagaa attgtgcctg
     1081 ttacagtaaa attaaaatag ttatttatag taaaagacaa gctgctgctt ccgccaatat
     1141 aggtttcagg gaaattaagt tccgtaacag aaataggatt aatacccaag agttcgggca
     1201 aagaatattt aactccttgc aatatttctt tagtagcatt attctgcaga aaaagaactg
     1261 cctcacagga agttatatcc cattcaagat taaatattcc ggtaagatta taggtctgct
//
LOCUS       DAOPFL010000246         1313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_151023_length_1313_cov_83.972973, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000246 DAOPFL010000000
VERSION     DAOPFL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151023_length_1313_cov_83.972973"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLG69_08365"
     CDS             <1..180
                     /locus_tag="PLG69_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43554.1"
                     /translation="RNSGLAKFNSVQDTIFLDTIIYFGYKEKNPYRQGDQDEKVFIFS
                     INLIACYLVWTGRLW"
     gene            110..>1313
                     /locus_tag="PLG69_08370"
     CDS             110..>1313
                     /locus_tag="PLG69_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43555.1"
                     /translation="MKKYLFLVLILLPVILFGQAGYGEISIPGDPMWEEESETTEGTH
                     WGMGGAVGAVTIDNQTYSQIRLQPELQVWKFGLGLDIDLIIDAEGKVRKEDWDEWQDY
                     VNKILFLRFAQRNDPFYFKVGSIPDYTLGNGLIFNHYSNMTLYPQVKNVGGYVGINTH
                     LSGLGFEAYTHNLHKNEILAGRVHFQPLAVTNIPLLEKLRVGVNVGIDRDQYGKYEDK
                     DGDNIPDVYDKFPKDKNRYLDTDNDGIADNDDIDIDGDNIFDDPSVNTFVRDHFTVIE
                     VLDYELDMDVKPDIATAYTEKDEVLVYSVDYQLPLIESERFNLIHYGEIAKIDGYGTG
                     MIFPGFSSKFFIFDARLEFRNFGDQFLPGYFDRQYDQQRAQVRHIISDGNGNQIWYLT
                     TKEETLKDV"
BASE COUNT          424 a          191 c          293 g          405 t
ORIGIN      
        1 cggaattccg gtttggcaaa attcaatagt gtgcaggaca cgatttttct tgacacaata
       61 atatactttg gctataaaga aaaaaatcct tatagacaag gagaccaaga tgaaaaagta
      121 tttattttta gtattaatct tattgcctgt tatcttgttt ggacaggcag gttatggtga
      181 aataagcatt ccaggtgacc caatgtggga agaggaatca gaaactacag aaggaacaca
      241 ttggggaatg ggaggagctg ttggagccgt tacaattgat aatcagacct attctcagat
      301 tcgtttacag cccgaacttc aggtttggaa atttggatta ggtttggata ttgatttaat
      361 aattgatgca gaagggaaag tgcggaaaga ggactgggat gaatggcagg attatgtaaa
      421 caaaatttta tttctcagat ttgctcaacg caacgatcct ttctatttta aggttggcag
      481 tattccagat tatactttag gtaatggttt gatttttaat cattattcca atatgacgct
      541 ctatcctcaa gttaaaaatg taggtggtta tgtaggaata aatactcatc tttcgggatt
      601 aggttttgaa gcatatacgc ataacctgca caaaaatgag atcttagccg gtagagtgca
      661 ttttcaaccc ttggcagtaa ccaatattcc tctgctggag aaattgcgag taggagttaa
      721 tgtaggcata gatcgtgacc aatatggaaa gtatgaagat aaagatgggg ataacattcc
      781 ggatgtttac gataaatttc ccaaggataa aaacagatat ttggataccg ataatgacgg
      841 gattgcggat aatgatgata tagacataga tggagataat atttttgacg atccttctgt
      901 taacactttt gtacgggacc attttacggt aatagaggtt cttgattatg agttagatat
      961 ggatgtaaaa cccgatattg caactgccta taccgaaaaa gatgaagttc ttgtttatag
     1021 tgtagattac cagttaccct taatagaatc cgagcgtttc aacctgattc attatggaga
     1081 aatagccaaa attgacggtt atggcacagg tatgatattt cccggattca gcagtaagtt
     1141 ctttattttt gatgcccgtt tggaattccg caattttggt gatcaatttt tgccgggata
     1201 ttttgacagg caatatgatc aacagcgggc tcaggtaagg catataattt ctgatggtaa
     1261 cggaaatcaa atatggtatt taacgactaa agaagaaaca ctgaaagatg ttg
//
LOCUS       DAOPFL010000247         1307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_152115_length_1307_cov_81.975240, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000247 DAOPFL010000000
VERSION     DAOPFL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152115_length_1307_cov_81.975240"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1060)
                     /locus_tag="PLG69_08375"
     CDS             complement(155..1060)
                     /locus_tag="PLG69_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43556.1"
                     /translation="MRKYRLILCLLLIIIELSAFQVYLPEGQVKEITKEDLSKEKLVS
                     FTTIRDKDGGQKEETWQGIPLIPWLNSLTSSPWQNLQIESNDNFMLTFHRLELDDNNA
                     YLALKLGAEELSEYNIRLIFTRYRENAWMRNIRCLRLLNFIPVPKPKQIYFWEKGLQK
                     NGIDLKSGAIAMKDLMQKGFSLESGDLIFVDEQFRKLCLNYPREIADASLFMDSQGQY
                     FLVPESSSALKISFENDLSRIIYIQCGAIAYLAEDTIENMEKIAQTLEWDWQGDYLYL
                     IKAEKQKIKAGEKPMDINADCWLEF"
BASE COUNT          391 a          253 c          240 g          423 t
ORIGIN      
        1 aaagtgatgt ttatgctggt ggaacaatat attatgtttt agagtgctgg ggtgcgggag
       61 tgcgggagtt aaatggtaag ttgagataat aatcttttct cggtgttctc ggtgtaaaaa
      121 taaaagctaa gcaatctgcg tgagatagtt cactctaaaa ttccagccag caatccgcat
      181 tgatatccat cggtttttca cctgctttta ttttctgttt ctcggcttta attaaatata
      241 aataatcccc ctgccagtcc cattcaagag tttgggctat tttttccata ttttctatcg
      301 tgtcctcagc aagataagca attgctccac actggatata gataattcgg gaaaggtcgt
      361 tttcaaaaga gattttgaga gcagaagaag attccggcac cagaaaatat tgtccctggg
      421 aatccataaa tagagacgca tcggctattt cacgaggata attcaaacat aacttccgaa
      481 attgttcatc cacaaagata agatccccgc tttccaagct aaaacctttt tgcattaagt
      541 ccttcattgc aattgctccg gattttagat caatgccgtt tttctgcaaa cccttctccc
      601 aaaagtatat ttgtttgggt ttgggcacag gtataaaatt cagcaaacgc aagcatctga
      661 tatttctcat ccaggcattt tcccggtagc gggtaaaaat taagcggata ttatattcag
      721 aaagctcttc tgcacccaat ttcaaagcca gataagcatt attgtcatcc aattctaaac
      781 ggtgaaaagt tagcataaaa ttgtcattac tttcaatttg caaattttgc cagggactgc
      841 tcgttaaaga attgagccag ggaattaaag gaataccttg ccaggtttct tctttttgtc
      901 ctccatcttt atctctgata gtagtgaaag aaacgagttt ttctttgctt aaatcctctt
      961 tggtaatttc ctttacctga ccttccggta aatatacctg aaatgctgat agctcaatta
     1021 ttattaacag cagacacaga attaaacggt atttacgcat agcatcatcc ttttttacac
     1081 tttatactaa tataaataat cagtataacc caaaaaacaa atacagtgct ggagtgctgg
     1141 agtgctggag tgctggagtg ctgaagtgcc ggagtgctgg agtgcagaag ttaaatataa
     1201 ggtacaatat ttccatatag ttatacttct gcatatcata cttcatttgt cattttctgt
     1261 tcttagtcct ctctgccaaa cacaaataaa atctgcgaaa tctgcgt
//
LOCUS       DAOPFL010000248         1283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_156572_length_1283_cov_98.775244, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000248 DAOPFL010000000
VERSION     DAOPFL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156572_length_1283_cov_98.775244"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..180)
                     /locus_tag="PLG69_08380"
     CDS             complement(10..180)
                     /locus_tag="PLG69_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43557.1"
                     /translation="MSFLNKQECRNVWKFERVDKVEKVEKVEKFERVDKVEMLSALSS
                     LPSAKKARRATQ"
     gene            complement(204..845)
                     /locus_tag="PLG69_08385"
     CDS             complement(204..845)
                     /locus_tag="PLG69_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPI43558.1"
                     /translation="MKKYVFITLGLLALAFCFAEESPVTLYLQNQNLETFQQAVNYLI
                     KIDQEGESGMQAKLNLAYISNLESLRLMDLAKADIENLKPGERFALANLYLEMDKYDD
                     AIDIYNLLNEATPKWSCPWRHKGEALYKSGNYEAAKKSLEMAIETNNEHYDAYLWYAK
                     TLYELKDYKNALKALETAFDLTEELEDSGFDQVIPEEEINQLYQELQKLTQNK"
     gene            complement(860..1141)
                     /locus_tag="PLG69_08390"
     CDS             complement(860..1141)
                     /locus_tag="PLG69_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPI43559.1"
                     /translation="MLTVNEYFEGKVKSIAFQNEQGNFTVGVMDIGEFEFGTNTIERM
                     TVISGELTVQLPEEETWKKFTSTETFTVPAHKKFRVKVQEQTAYLCRYE"
BASE COUNT          334 a          282 c          228 g          439 t
ORIGIN      
        1 ccatcgctat cattgtgtcg ccctccgggc tttttttgca gagggcagag agctgagggc
       61 agagagcatc tcaaccttat caaccctctc aaacttctca accttctcaa ctttctcaac
      121 cttatcaacc ctctcaaact tccaaacatt cctgcattcc tgcttgttca ggaaggacaa
      181 tgtatttttt gccagaaatg gtattactta ttttgggtta atttctgcag ttcctgataa
      241 agttgattga tttcttcttc cggaattacc tgatcaaatc cgctatcttc aagttcttca
      301 gttaagtcaa aagcggtttc cagagcttta agagcgtttt tatagtcctt taattcgtag
      361 agggttttgg cataccagag gtaagcatcg taatgttcat tattggtttc aattgccatt
      421 tccagtgact ttttagcagc ttcataattt ccgcttttat aaagggcttc tcctttatga
      481 cgccagggac agctccattt tggtgttgct tcgttcaaaa gattataaat atcaatagca
      541 tcgtcatatt tatccatttc cagataaaga tttgccaggg caaagcgttc gccgggtttc
      601 aaattctcaa tatccgcttt tgccaaatcc attagccgga gagattccaa attgctgata
      661 taggcaagat tcaattttgc ctgcattccg ctttcacctt cctgatcaat tttgatgaga
      721 taattaactg cttgctgaaa ggtctccaga ttttgatttt gcaggtataa agtgaccggt
      781 gattcttctg caaaacagaa agctaacgcc aacaatccca atgtgataaa tacatatttt
      841 ttcattttat caatcctcat tattcataac ggcaaagata tgctgtttgt tcttgtactt
      901 ttacccggaa ttttttatgg gcaggaacgg tgaatgtctc ggtggaagta aattttttcc
      961 aagtttcttc ttcgggtaat tggacggtaa gttctccgga aataacggtc attctttcaa
     1021 ttgtattagt gccaaattca aattccccaa tatccattac tcctacagtg aagttgcctt
     1081 gttcgttttg aaaagcaatg gactttactt tgccctcaaa atattcgttt acagttagca
     1141 tttttgcctc cttaatactc attttttgat tgcaccattc tcgcaaaact taaattccgg
     1201 ttataaaaaa gtggctccgg aagaggggtt cgaacccccg acctagtggt taacagccac
     1261 ccgctctacc gctgagctat tcc
//
LOCUS       DAOPFL010000249         1247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_163620_length_1247_cov_98.439597, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000249 DAOPFL010000000
VERSION     DAOPFL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163620_length_1247_cov_98.439597"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLG69_08395"
     CDS             <1..351
                     /locus_tag="PLG69_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43560.1"
                     /translation="IPSMLILHHPVFSSGEHNDELGLQLILPELLQNTSVKAVFSAHD
                     HNYERSEFKGITYIVTGGGGAPLREKVHENPYSLVFVKTHHYLIGEREDKSIIFKVFN
                     LDDELIDSFTINGM"
     gene            353..1204
                     /locus_tag="PLG69_08400"
     CDS             353..1204
                     /locus_tag="PLG69_08400"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HPI43561.1"
                     /translation="MPHPKVLAIHDLSGFGNTSLMAIIPIMNRMGIEVTAMPSALLSA
                     NTDYPDYILQDNSELLKKSLTHWKELQLSFDAVYTGFLGSPAQVYLLLENLPSLLKKE
                     GIVLVDPVLADEGKLYSCYTWEMVEAMRSLVSISHIITPNWTEAVFLSGYKNGKDLKT
                     VRVYECCKKLATLGPQNIVITSAPDSQGNFSSVAFFAAENNFFREFECNYAPVIFPGA
                     GDCFSALLLAGILNGCDIVQSIEGTIAFLEEAITISIPVVQDRKTGIDLISALKKDPH
                     LFYSQKE"
BASE COUNT          365 a          235 c          237 g          410 t
ORIGIN      
        1 attccttcaa tgcttatttt acatcatccg gttttttcat ccggagagca caatgacgag
       61 cttggtttac agcttatttt gccggaatta ttacaaaata catccgtaaa agctgttttt
      121 agcgctcatg atcataatta cgagcgttcc gaatttaaag gtataactta cattgttacc
      181 gggggtggag gagctccttt aagggaaaaa gttcacgaaa atccctattc tttggttttt
      241 gtaaaaaccc atcactatct tattggagag cgagaagata agtccattat ctttaaggtc
      301 tttaacttgg atgatgagct catagatagc tttactataa acggaatgta atatgcccca
      361 tccaaaggtt ttagcaattc acgatctttc cggttttggt aacacttcct taatggcaat
      421 tatccctatt atgaacagga tgggaattga agttaccgct atgcccagtg ctttactttc
      481 tgccaatacc gattatcctg attatattct gcaggataat agcgagcttt taaaaaaatc
      541 cctaacgcac tggaaagaac ttcagcttag ctttgatgcc gtttataccg gttttttagg
      601 atctcctgct caagtatatc tcttacttga aaatttgcct tctctactta aaaaagaggg
      661 aatcgtttta gttgaccccg ttttagcgga tgagggaaaa ttatatagtt gttacacttg
      721 ggaaatggta gaggcaatgc gttctcttgt ttcaattagt catattatta ctcccaattg
      781 gacagaagca gtttttttaa gcggttataa aaatgggaaa gacttaaaaa ccgtccgagt
      841 atatgaatgc tgtaaaaaat tagccacttt aggacctcaa aatatagtta taaccagtgc
      901 ccctgattct caaggaaatt tttcctcggt tgcctttttt gctgctgaaa acaatttttt
      961 ccgggaattt gaatgtaact atgcccccgt aatcttccca ggagcaggtg attgcttttc
     1021 tgcattgctt ttagccggta ttttaaatgg ctgtgatatt gttcaatcaa tagaaggaac
     1081 tatcgccttt ttggaggagg caataacaat tagcatccct gttgtgcagg atcgtaaaac
     1141 gggcattgac cttatttctg ccttaaaaaa agacccccac cttttctatt ctcaaaaaga
     1201 gtaaccgcgt ttcttttcta caaagagaaa agagaaaaag ggtttag
//
LOCUS       DAOPFL010000250         1229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_167464_length_1229_cov_85.391823, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000250 DAOPFL010000000
VERSION     DAOPFL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167464_length_1229_cov_85.391823"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLG69_08405"
     CDS             <1..441
                     /locus_tag="PLG69_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43562.1"
                     /translation="NGNQIWYLTTKEETLKDVESSLGWFGYLRANLFNIVYAKIAYQD
                     MYGKQSFLGKSLWASVTVCPSIVPKLKEASVCYAQTNVNYINFKYPRNDNANILGRLV
                     YSISDNANLVGSYREYYTDINNDGKIKGKDEIIETFTFGVEFWF"
     gene            complement(456..683)
                     /locus_tag="PLG69_08410"
     CDS             complement(456..683)
                     /locus_tag="PLG69_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43563.1"
                     /translation="MTNLQFIRRRKPLAKFLFYQLNIVFSVPWKAPILKDKLMLGRTS
                     VNSRERYIIVLVNKEFSAIYFRSLKLRIIQA"
     gene            complement(841..>1229)
                     /locus_tag="PLG69_08415"
     CDS             complement(841..>1229)
                     /locus_tag="PLG69_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HPI43564.1"
                     /translation="QGAGIEIMANSDNVIRGGLTPKYINREILFAITRFETYLPQLIH
                     PHMKINELVSYPTPAEEFYLSRLILEGTYILDNLHKPLLVFCFKGSFTTEDGFSLERG
                     KAMFISFAERPVTLSGSADLFIASIP"
BASE COUNT          403 a          210 c          243 g          373 t
ORIGIN      
        1 aacggaaatc aaatatggta tttaacgact aaagaagaaa cactgaaaga tgttgagtcc
       61 tctctcggct ggtttggcta tttaagagcc aatcttttta atattgtcta tgctaaaatt
      121 gcctatcagg atatgtatgg caagcaaagc tttttgggca aatccttgtg ggcaagcgtt
      181 actgtatgtc cttctattgt tcctaaattg aaggaagcat cagtttgtta cgctcaaacc
      241 aatgtaaatt atatcaactt caaatatccc agaaatgata atgccaatat tttagggcgc
      301 ctggtatatt ccatttcgga taatgccaat ttggttggaa gttacagaga atattatacc
      361 gatatcaata atgacggtaa aatcaaaggt aaagatgaaa taattgaaac cttcactttt
      421 ggagtggaat tttggtttta ggcattaacc aatatctatg cctgaataat cctcaatttt
      481 aaggaacgga aatagatagc agaaaattct ttattcacaa gaacaatgat atagcgttct
      541 ctactgttaa ctgatgttct acccaacatc aacttatcct taaggatggg tgctttccaa
      601 ggaacagaaa agacaatatt taattgataa aacaggaatt tagcgagggg cttacgcctg
      661 cggatgaact gcaagttcgt cattcacttg tacctcaact atttcctgat tattaatttg
      721 gggtaataaa gtagattgga aagtatagtt gaagaggatt tcaagcttcc gggttctaaa
      781 ccttttcaac cttttcaacc tttttaagcg ggatttgata aatatgttgc tataaatata
      841 tcagggaatt gacgcaataa agaggtctgc agaaccggat agagttacag gtctttccgc
      901 aaaagagata aacattgcct tacctctttc caacgagaaa ccgtcctctg tagtaaaaga
      961 acccttaaaa caaaagacca gcaagggttt atgcaaatta tccaaaatgt aggttccttc
     1021 cagaataagc cgtgaaagat agaattcttc tgccggagta gggtatgaaa ccagttcatt
     1081 aattttcata tgcgggtgaa ttaattgggg caggtatgtt tcaaaacggg tgatggcaaa
     1141 aagtatttcc cggtttatat atttgggagt aagtcctccg cggataacat tatcggaatt
     1201 agccataatt tcaattccgg ctccctgga
//
LOCUS       DAOPFL010000251         1213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_170984_length_1213_cov_112.601900, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000251 DAOPFL010000000
VERSION     DAOPFL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170984_length_1213_cov_112.601900"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1213
                     /locus_tag="PLG69_08420"
     CDS             <1..>1213
                     /locus_tag="PLG69_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPI43565.1"
                     /translation="GSYGYLLQQISLSGISSQNSYRLCLNAYNADNDFPIPKGDLIPE
                     DSENKRKNNSKQQLSFSGAYSTHLSKLLMSISTDYLTFQRELPGPLNFADLYYKAFLE
                     GSSLRPQIRFSANFGNFTWQSNNWLLKDETVYDNTQSLIEGFSQKYKQKNDNYGIKQS
                     LSYNKNSLQASLSGEYVYNRYRYQDLINPFQGKIDYVRNQLAFSLKLNQQKEFAWLIF
                     NSGLAGRYDYIDGEDEFSGRLEFSLRNWGTKYLETGATIGNSFALPTPYDLYWKGDSQ
                     TLGNPNLKSEKATGYQIWLKGNIPQATLKATLHQNTIKNLIQWRQVQMYGPVWKPMNI
                     GRAEIRNLELEGNYQPLKKLELTASAVFTKAKDTTCHSFAEAPKLMYRPETLYSLAVD
                     YQPGIFHFWTK"
BASE COUNT          379 a          249 c          234 g          351 t
ORIGIN      
        1 tcggttctta tggctatttg ctgcaacaaa tttccttaag cggaatcagt tctcaaaatt
       61 cctatcgttt atgcctaaat gcctataatg cagataacga ttttcctatt cccaaagggg
      121 atttgattcc tgaggatagt gaaaacaaac gcaaaaacaa tagcaaacag cagctttctt
      181 tttcgggtgc gtattccact catctatcta aattgctgat gagcatttct accgattatt
      241 taacctttca gagagaactt cccggtcccc ttaattttgc cgatttatat tataaggcat
      301 ttttagaggg ctcatccctg cgtccgcaaa ttcggttttc cgctaatttc ggcaatttta
      361 cctggcaaag caataattgg ctgctgaagg acgaaacggt ttatgataat acacagtcac
      421 taattgaggg cttttcccaa aaatacaagc aaaaaaatga taactatgga ataaagcaat
      481 ctctcagcta taataaaaac tctttgcagg catctttatc cggggaatat gtgtataacc
      541 gttatcgcta tcaggattta attaaccctt tccagggaaa gatagattat gttagaaacc
      601 agttagcttt tagcttaaaa ctaaatcagc aaaaggaatt tgcctggcta atttttaata
      661 gcggattggc gggtcgttat gattacattg atggtgaaga tgaatttagc ggtcgcctgg
      721 aattttccct tcgcaattgg ggaacaaaat atctggaaac gggagctaca ataggcaatt
      781 cttttgctct accaactcct tatgaccttt actggaaagg ggattcccaa accttgggta
      841 atcccaacct caaaagtgaa aaagcaaccg gctatcaaat ttggctgaaa ggcaatattc
      901 cccaagcaac tctaaaggca accttgcacc aaaatacgat taaaaacctg attcaatggc
      961 gacaagtgca aatgtatggt cccgtttgga agccgatgaa tattggtaga gcggaaattc
     1021 gtaatctgga gctggaggga aattaccaac cgcttaaaaa attggaactt acggcttcag
     1081 cggtttttac gaaagccaag gatactacct gccacagttt tgcagaagca cccaaactta
     1141 tgtaccgtcc tgaaaccctc tattctttag cggttgatta tcaaccggga atctttcact
     1201 tttggacaaa ata
//
LOCUS       DAOPFL010000252         1200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_173917_length_1200_cov_77.314410, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000252 DAOPFL010000000
VERSION     DAOPFL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173917_length_1200_cov_77.314410"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLG69_08425"
     CDS             complement(<1..203)
                     /locus_tag="PLG69_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43566.1"
                     /translation="MKQIKLVLIALILMISLSSVFAEDNSAAVYQLLGIDARSNGMGG
                     TGTAFLDNVSSAYLNPAVLADVKT"
     gene            complement(261..>1200)
                     /locus_tag="PLG69_08430"
     CDS             complement(261..>1200)
                     /locus_tag="PLG69_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPI43567.1"
                     /translation="FTDIAWLNSVGPNNLNFTINAVVPVTVTANVITYPSGATIMGPL
                     TVNPENGIYTVILNGGMIPAGETSVRLQVIATDQFGNVTEANHYYGVDRSAPVITILN
                     PVNGAEITLVDETTKVRIEAQFSEFVPLLKNGGKNSSGSGIAGSRMVIIDPNGMPVGV
                     PVETGAGITEVSHEIDNLILGTYTVRVTAWDNAGNQAMETISFTMISAPLPPAELAIA
                     DAYVYPNPSIDGTAKFAITLTNGAYVKIQIYDFAGREVRTLSSSGKVQGKSKAEIVFD
                     GRNNDGVKLARGAYFARVIANDGTKIVEKVVKIAIK"
BASE COUNT          346 a          283 c          227 g          344 t
ORIGIN      
        1 gttttaacat ctgccaaaac tgccggattc aggtatgcgc ttgagacatt atccaaaaac
       61 gcggttccag ttccacccat accattagaa cgggcatcta taccgagcaa ttgatatact
      121 gctgcagaat tatcttcagc aaagacagaa gacagtgaga tcatcagtat cagggctatc
      181 agtactaatt taatttgttt catttttttc cttcctttcg gttggaaggg agggggagtt
      241 cccctcccct cttcaccaat ttacttgata gctatcttaa ctaccttttc tactatcttc
      301 gtgccgtcgt tggcaatcac acgggcaaaa tatgctccgc gcgctagttt tacaccatca
      361 ttattacgac cgtcaaaaac gatctctgct ttactcttac cctgcacctt gccacttgaa
      421 cttaaggtgc gaacttcacg acccgcaaag tcatagattt ggatctttac ataagcacca
      481 ttagtcaaag taatggcaaa tttggcagtt ccatctatgc tgggattcgg atatacataa
      541 gcatccgcaa tcgccagttc agcaggaggt agaggagctg aaatcatcgt gaaactaatt
      601 gtttccatag cttggttacc tgcattatcc catgcagtta cacgcacggt gtaagttcca
      661 agtatcagat tatctatttc gtgacttact tcagtaatac ctgcacctgt ttcaacagga
      721 actccaacag gcattccatt aggatcaata atcaccatac ggcttccagc aattcctgaa
      781 ccggaactgt ttttgccgcc attcttcaga agaggaacaa attcactgaa ttgggcttca
      841 attctaacct tagtggtctc atccaccaaa gtaatttcag caccatttac aggattaaga
      901 attgttatta ctggagcaga acgatccaca ccataatagt ggttagcttc agttacatta
      961 ccaaattgat cggtagctat tacttgcaag cgaacagagg tctcacccgc aggaatcata
     1021 ccaccattca atattacagt gtaaatacca ttctccggat ttacggtgag cggacccatt
     1081 atggttgctc ccgaaggata ggtaattaca tttgcagtaa ccgtaacagg aactactgca
     1141 ttaattgtga agttaaggtt attaggacct acggaattca accaggcaat atcggtgaag
//
LOCUS       DAOPFL010000253         1197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_174592_length_1197_cov_128.510508, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000253 DAOPFL010000000
VERSION     DAOPFL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174592_length_1197_cov_128.510508"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLG69_08435"
     CDS             complement(<1..671)
                     /locus_tag="PLG69_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPI43568.1"
                     /translation="MKDLQLITEEHIPLLQEFLTRYPRRNCDFNLTNLLAWGKIYRNH
                     YLLWQDNLVIFNPKYQNVCFPLGENFTPKDLSDLVILFKQEYPEAELIIVPEEYYAKN
                     PELDNFFTVREERAWADYVYKIDKLVQLSGKKLAKKKNLISQFIRAYPDYQVLPVTSD
                     KFEVLLRFTYKWKRERSAEGIYLMTEIKAIENTLAMWDLLPAEGIIICLHDKIAAFSI
                     FSPQTP"
     gene            complement(675..1091)
                     /locus_tag="PLG69_08440"
     CDS             complement(675..1091)
                     /locus_tag="PLG69_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPI43569.1"
                     /translation="MLQVNYKIVQETDTKAILELYQQAGWWQSESSDNDLDIIAKIVA
                     NSFCFVIAILDGKIVGMGRAISDGISDAYIQDVTVQKEYRGKGIGKGIMRTLIKFLKE
                     NNLKWIGLISEPGYESFYQGLGFKVMTSYTPFLLSE"
BASE COUNT          375 a          245 c          189 g          388 t
ORIGIN      
        1 ggtgtctgag gtgaaaaaat agaaaaggca gcaattttat cgtgcaggca aataattatt
       61 ccttccgcgg gtaaaaggtc ccacattgcc agagtgtttt caattgcctt aatttccgtc
      121 attaaataaa taccttctgc actgcgttcc cttttccatt tataggtaaa acgcaacagc
      181 acctcaaatt tatcgctggt tacaggcaaa acttgataat cgggataggc acgaataaat
      241 tgggaaatga ggttcttctt ttttgccaac tttttaccgg aaagctgaac taatttatct
      301 attttataaa cataatctgc ccaagctctt tcttcccgca cagtaaaaaa attatccagt
      361 tcaggatttt tggcataata ctcttccgga acaatgatca attccgcttc gggatattcc
      421 tgcttgaaaa gaattactaa atcagacaag tccttcggag tgaaattttc tcctaaagga
      481 aaacagacat tctgatattt gggattgaaa attaccaggt tatcctgcca caaaagataa
      541 tgattccgat aaatctttcc ccaggcaagg agattggtta aattgaagtc acaatttctt
      601 ctcggataac gggttaaaaa ttcctggagc agaggaatat gctcttctgt gattaattgc
      661 aaatccttca tctattattc acttaacaaa aatggagtgt aggaagtcat aactttaaag
      721 cccaaaccct gataaaaact ctcataacca ggttcgctga taagtcctat ccatttcaga
      781 ttattttctt ttagaaactt tatcaatgtg cgcataattc ctttgccgat acccttaccc
      841 cgatattcct tttgcacggt tacatcctga atataagcat cacttattcc atcactgatg
      901 gctctaccca taccgacaat tttaccatct aagatagcaa taacaaaaca aaaggaattg
      961 gcaacaattt tagcaataat atctaagtca ttatcactgc tttctgattg ccaccatccg
     1021 gcttgttgat atagctctaa gatagctttt gtatccgttt cctgcacaat tttataattt
     1081 acttgtaaca tcaatattta catcctttta gcagttagag tttatattct gcaaaagtaa
     1141 actatagatg gatgaactga tttttgtcaa gctccgtttt tgtacctctc acagatt
//
LOCUS       DAOPFL010000254         1194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_175295_length_1194_cov_64.320457, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000254 DAOPFL010000000
VERSION     DAOPFL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175295_length_1194_cov_64.320457"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PLG69_08445"
     CDS             complement(<1..1175)
                     /locus_tag="PLG69_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPI43570.1"
                     /translation="MKASRFFLLLIVTILLLPISLSGNTNGEINPFKIKEINDSEIRI
                     QFTLPQWEIEQINVKNEIRKKVKVQETPYLFIDEEETLPVFSTMIAIPNRGGVDLLVS
                     NSSKSSINDFTADFDAALNRENQQGRFTDIHYPAANVVISEPQILRDFRVVNLNIYPF
                     QYDRINRKLLVSENLDIKLTFNSRPSINELNSSSYISCSFDSIYRGLILNYESLRQTR
                     EIDYRNPVMLVIYGNYTDTNYLSKINEFVTWKKQRGFIVNAVSTGVTGITNTNIKSYI
                     QTAYNNPATRPDYIVLIGDTDGTIAVPSNNTYVDYQYTWLAGSDNLGDVMIGRISVTS
                     TADLDVILAKIISLEKNIGQHPTDWLNRMVLVGDTGSSGISTIYTNRYIRDISEEINP
                     "
BASE COUNT          380 a          225 c          202 g          387 t
ORIGIN      
        1 ggattaattt cttccgaaat atcacgaatg tatctattag tataaatcgt tgagatacct
       61 gaagaaccgg tatccccaac taaaaccatt ctatttaacc aatcagtggg atgctgtcca
      121 atgttctttt ccagagagat gatttttgcc aggataacat ccaaatcagc agtagaagtt
      181 acagaaattc taccaatcat cacatcaccc aggttatcac ttcctgccag ccaggtatat
      241 tgatagtcaa cataagtgtt attacttgga acagcaatag tgccatctgt atcaccaatt
      301 aagacaatat agtccggacg cgttgcagga ttgttgtaag ctgtttgaat ataactctta
      361 atattggtat tggttattcc ggtaacacca gtgctaactg cgtttacaat aaagcccctt
      421 tgctttttcc aggtaacgaa ttcatttatt ttagataaat agttggtgtc tgtataattg
      481 ccatagataa ccagcatcac aggattccga tagtcaattt cccgtgtttg ccgcagggat
      541 tcatagttta aaatcagccc tctatagata ctgtcaaaac tacaagaaat ataagaactg
      601 gaattcaatt cattgataga aggtctgctg ttaaatgtta actttatatc cagattttca
      661 ctaacaagca gtttccgatt tatccggtca tattgaaagg gatatatatt aagatttaca
      721 accctgaaat cacgcaatat ctggggctct gagataacaa cattagcggc aggataatga
      781 atatcggtaa atcttccctg ttggttttcc ctattcagag cagcatcaaa atcggcagta
      841 aaatcattaa tagaagattt tgaggaatta gaaacaagca gatctacccc tcccctattc
      901 ggaatagcaa tcattgtgga aaaaaccggc aatgtttctt cttcatctat aaaaagataa
      961 ggtgtttcct gaactttaac tttctttctt atttcgtttt taacattaat ttgttcaatt
     1021 tcccattgcg gtaaagtaaa ttgaattctt atttcgctat catttatttc cttaatctta
     1081 aaaggattga tttcgccgtt tgtattgccg gatagcgata ttggcaataa caaaatagtt
     1141 acaattagca gcaggaaaaa tcgggacgct ttcattaaga acctctatat tcgt
//
LOCUS       DAOPFL010000255         1179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_178810_length_1179_cov_112.992883, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000255 DAOPFL010000000
VERSION     DAOPFL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178810_length_1179_cov_112.992883"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /gene="rnpB"
                     /locus_tag="PLG69_08450"
     ncRNA           complement(<1..251)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG69_08450"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(409..1140)
                     /locus_tag="PLG69_08455"
     CDS             complement(409..1140)
                     /locus_tag="PLG69_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPI43571.1"
                     /translation="MEEQLRTLAQMQTLDDEIGRYRVLQKELPKQLNEIIESVEQATA
                     ILLEVETQRAEINKKQRTLEMDIKQLQEQHKKYTNQLAEIKNNKEYKALNSEIAYIKD
                     KISDLESNLLELMDQENEIKEKIASAKAELEKAETRKKEKETDLREQIASLETKIEDV
                     RNKRNDLARTLPVPVVKHYGNMIKHKNNQAVAYNRNGSCGACGFVIRQQMRIELQLRR
                     KIIYCENCGRILMNRFEDNSEDASN"
BASE COUNT          290 a          244 c          210 g          435 t
ORIGIN      
        1 tgcgggcaac atcatagctt tcctatttgg cattgcaccg gataaggcat tcctgtctat
       61 ctttgttacc aaagataccg gtggtctctt acaccaccat ttcaccctta ccatttccga
      121 acaaaaaaat ggcggtttat ttctgtggag cttgctctgc attactgcag cttcctgtta
      181 ggaagtatcc tgccctatgg tgtccggact ttcctctgca ttttgaatgc agcgaacatc
      241 caatcttccc tctataaatt ataattcaaa actaataaaa accaaatttc cccgtaaaca
      301 atttattgtt ccatatagtc atttatcatt cctgcgaagg gaggaatcca gataaaatgt
      361 agcacaatag ctatgttttc agaacaacaa ttataataga acttcagatt agttagaggc
      421 gtcttccgaa ttatcctcaa aacggttcat cagaatgcga ccgcaatttt cacaatagat
      481 aattttgcgt cgcaattgaa gttcaattct catttgttga cgaatcacaa aaccgcatgc
      541 accacaagaa ccattgcgat tgtaagcaac tgcctggttg tttttatgtt taatcatatt
      601 tccgtaatgt ttaaccacag gaacgggcaa ggtgcgtgct aggtcatttc ttttattgcg
      661 aacatcttca atcttggttt ccagagaagc aatttgttct cttaaatcgg tttctttttc
      721 cttttttctg gtttcggctt tttccagttc agctttggca gaggcgatct tttctttaat
      781 ttcgttttcc tggtccatca attccaaaag gttggattcc agatcggaaa ttttatcttt
      841 gatataggca atttcgctgt ttaatgcctt atattccttg ttgtttttga tttctgccag
      901 ttgattggta tattttttgt gttgttcctg cagttgtttt atgtccattt ccagggtgcg
      961 ttgttttttg ttaatttctg ccctttgggt ttccacctca agaaggatgg ctgttgcttg
     1021 ttctacgctt tcaataattt cgttaagttg tttgggcagt tctttttgta aaaccctata
     1081 gcggccaatt tcatcatcca gggtttgcat ttgggctaaa gttctcagtt gttcttccat
     1141 agtttactcc ttaaataaca aactgatatc cagtgactt
//
LOCUS       DAOPFL010000256         1169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_181260_length_1169_cov_67.580790, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000256 DAOPFL010000000
VERSION     DAOPFL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181260_length_1169_cov_67.580790"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLG69_08460"
     CDS             complement(<1..292)
                     /locus_tag="PLG69_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPI43572.1"
                     /translation="MPDSLHTIAQPIVATMASQNLAYFLAWIGLFLMVALSGVGSAWG
                     TVIGGRASVGAMKKRDDVFPSCMILSALPGTQGLYGFGSFFILKAYVTPQINM"
     gene            complement(307..>1169)
                     /locus_tag="PLG69_08465"
     CDS             complement(307..>1169)
                     /locus_tag="PLG69_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPI43573.1"
                     /translation="LTPFFAPFFALFFGFCNADIGYGIVIIILAVILKLKMKNQGMRD
                     ILTLLMIFGTASIIMGWVMGSALAFDLKTIPAIKNKILIKDNNQIFNYALLLGAIQIL
                     FGIAIAAVKKMKKNGFKFGLSTIGTWLLLFGLTVLGSSLLGANISKVQPYIKYPIYIG
                     LAMILLFNSPGKNIILNILNGFWLLYQIVTGYFGDILSYIRLFALSVSSAILGFVMNS
                     IGSQIANGIPIVGPIIFVLFMLFGHTLNIALGGLSGFVHPLRLTFVEFYKNAAFEGPG
                     LEYKPFGKKE"
BASE COUNT          434 a          241 c          197 g          297 t
ORIGIN      
        1 gcatattaat ttggggagta acataagctt tcaggatgaa gaaagaacca aaaccatata
       61 gaccctgcgt tccaggtaaa gcggatagga tcatacagct gggaaaaaca tcatcccttt
      121 ttttcattgc accaactgat gcacgaccac cgataacagt tccccaggca cttcccactc
      181 cggaaagagc taccatcagg aaaagtccga tccaagccaa aaaatatgcc aaattttggc
      241 tcgccatagt ggcaacaatt ggttgagcta ttgtgtgtaa ggaatcaggc attttaacct
      301 cccttgttat tcctttttcc caaagggttt atattccagc ccgggacctt caaaagcagc
      361 atttttgtaa aactccacga aagtaagtcg taaaggatga acaaaaccgc taagacctcc
      421 caaggcaata tttaaagtat gtccaaaaag cataaacagc acaaaaataa taggaccgac
      481 aatcggaatt ccattagcaa tttgacttcc aatagagttc attacaaaac ctaaaattgc
      541 gctggaaacg cttaaagcaa aaagacggat gtaagataaa atatcaccaa aataaccagt
      601 aacaatttga taaagtagcc aaaatccgtt gagaatattg agaataatat tcttaccggg
      661 actattaaac agcaaaatca tagccaatcc gatataaata gggtatttaa tatagggctg
      721 aactttgctg atatttgctc ctaaaagcga agaaccgaga accgttaacc caaacaataa
      781 taaccaggta ccaatagtac ttagcccaaa tttgaaaccg ttttttttca tttttttcac
      841 tgctgcaatg gctatcccga aaaggatttg gatggcacct aaaagcaaag catagttgaa
      901 gatttgattg ttatccttaa ttaaaatctt attcttaata gctggaatcg ttttcaaatc
      961 gaaagctaaa gcactaccca tcacccaacc cattattatg gaagcagttc caaagatcat
     1021 taacagggta agaatgtctc tcataccctg gtttttcatc ttcaatttta agataacagc
     1081 caaaatgata ataactatac cataaccgat atccgcattg caaaaaccga aaaacaaagc
     1141 aaagaaaggt gcaaagaaag gagttaaat
//
LOCUS       DAOPFL010000257         1166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_181975_length_1166_cov_101.565257, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000257 DAOPFL010000000
VERSION     DAOPFL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181975_length_1166_cov_101.565257"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(499..>1166)
                     /locus_tag="PLG69_08470"
     CDS             complement(499..>1166)
                     /locus_tag="PLG69_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPI43574.1"
                     /translation="EAYSDLEGMMDIAEELIKHLALDIIGKDTFFYHNHQVCLSGEWK
                     RASLPDLVSEFVGQNVMNISFEELSAICKNKELVIPPGSEKGKLIALLFENFVEETLI
                     QPTFVRDFPKEVSPLAKAKPENPFVADRFELFIAGGEFANAFSELNDPLEQRVRLEEQ
                     AKLRALGIDDANVVDEDFLEALEYGMPPMGGQGIGIDRLVMLLTENDSIKEVILFPQM
                     KTT"
BASE COUNT          359 a          227 c          203 g          377 t
ORIGIN      
        1 tgtataatgc agtccctgta atttttttgc ctgcaaagag gtgaatatga aaaaaatggg
       61 gtgttcattt tattgtaata gtcaacaaca atagctatat cgtttatcaa gagaactatc
      121 cggagagaat ttagctttca ataagttagg cgaatgaaaa attagttaat caatttcaat
      181 tggctttact ttgtatataa gtaatacaat atctaaattg ataattcaga aatatacgga
      241 atattcacct caaatacctt gttagtcaga aaataactat gcaacaacta agtattttag
      301 tttataaaat cccgaaaatc cgaataatcc tgttcatccc agacctattt tatcttttac
      361 gaggggttta cgcacccatc gctatttttg tatcgccctg agggcttttc tgatgaatgt
      421 ttaatagatt tcttcggatt aaaaatatat ttttgtgggt attctatata ttactgattt
      481 atcaattctg atattatatt aggtagtttt catctggggg aagaggataa cttccttaat
      541 ggaatcgttt tcggttagca acataactaa gcggtctatg ccgattccct gtccccccat
      601 aggaggcatt ccgtattcca gtgcttctaa aaaatcctca tctacgacat tggcatcgtc
      661 aattcccaaa gcacgtaact ttgcctgttc ttccaaacgg actctttgtt ccaaaggatc
      721 attcaattca ctgaaggcat tggcaaattc gccgccggca ataaaaagtt caaaacggtc
      781 tgcaacaaag ggattttcgg gttttgcctt agccaaaggt gagacctctt tgggaaaatc
      841 acgcacaaaa gttggttgga taagtgtttc ttctacaaaa ttttcaaaga gcagggcaat
      901 cagttttcct ttttcggagc ccggaggaat aaccaactcc ttgtttttac agatagcaga
      961 aagctcttca aaactgatat tcataacatt ctgtccgaca aattcactaa ccaaatcggg
     1021 caaagatgct cttttccatt ctccgcttaa gcaaacctgg tgattatgat aaaagaaagt
     1081 atccttgccg attatgtcca aagctaaatg tttaatcagc tcttcagcga tatccatcat
     1141 cccttccagg tcggaatatg cttcgt
//
LOCUS       DAOPFL010000258         1164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_182404_length_1164_cov_49.878269, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000258 DAOPFL010000000
VERSION     DAOPFL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182404_length_1164_cov_49.878269"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..828)
                     /gene="ispE"
                     /locus_tag="PLG69_08475"
     CDS             complement(7..828)
                     /gene="ispE"
                     /locus_tag="PLG69_08475"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPI43575.1"
                     /translation="MLTASYAKINLFLELIGKLSNNYHQVNTILCSIDLFDLLSYEPL
                     ESPEIILTCNIPALVSTSNLVYKVASCLKEQFNIPAGIKIHLEKHIPVSAGLGGGSSN
                     AANCILALNEIWQLNLSKPEIHKIASQFGSDINFFLEGGTAKGENRGEVITKLSPIIL
                     NNILLVNPGIEISSAEAYQLAVLPKKGEQHKFNPSNLIGSCFNRLESGIRNAYPVIDN
                     IINSISNFGAKIAMMSGSGSTCFGIFDREDDLSTCQQYFDQKGFWTYTTKTRETS"
     gene            complement(788..>1164)
                     /locus_tag="PLG69_08480"
     CDS             complement(788..>1164)
                     /locus_tag="PLG69_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43576.1"
                     /translation="IASPRTDFLLEELKSRYDFVLVDSPPLIAVTDALILTKKVDMTY
                     LVVRCGFTDKGIIKRTKELMENIEGRIDGIIVNGIYVQKYYSKQNYYYYYYYYYYYYG
                     EEVPQKQKRNVNRFLRKNKSIS"
BASE COUNT          361 a          212 c          212 g          379 t
ORIGIN      
        1 tttttattat gatgtttccc gtgttttggt agtataagtc cagaaaccct tttggtcaaa
       61 atattgctgg caagtgctca aatcatcttc cctatcaaat attccaaaac aggtagagcc
      121 actcccgctc atcatagcta ttttagctcc aaaattgctg attgaattga taatattgtc
      181 aattacagga taagcatttc ggatgccgct ttccaaacga ttgaagcagg aaccgattag
      241 gttggaagga ttaaatttat gctgctctcc ctttttagga agaacggcaa gttgatatgc
      301 ttcagcactt gatatttcta ttccgggatt aactaatagg atattattca ggataatagg
      361 agatagtttt gtaattactt ctcctctgtt ttctcctttc gctgttcctc cttccagaaa
      421 gaaattgatg tcactgccaa actgagaggc gattttatgt atttctggtt tagataaatt
      481 cagttgccag atttcattta aagccagaat acaattagcg gcattactac ttccgcctcc
      541 tagtcctgca gaaacaggaa tatgcttctc taagtgaatt ttaataccgg caggaatatt
      601 aaattgttct tttaaacagg aagcaacctt atatacaaga ttagatgttg aaaccagagc
      661 gggaatattg caggtaagga ttatttcagg ggattctagg ggttcatagc tcaacaaatc
      721 aaacaggtca atactacaga gtatagtatt gacctgatga tagttatttg agagcttacc
      781 tattagctca agaaatagat ttatttttgc gtaggaagcg gttaacattc ctcttttgct
      841 tttggggcac ttcttcgccg taatagtaat agtaatagta gtaataatag taataattct
      901 gtttactgta atacttctgg acatagatac cattcacaat aatgccatct attctacctt
      961 caatattttc catcaactct ttagtgcgct tgatgatgcc tttatcggta aaaccacaac
     1021 ggacaacaag ataggtcata tctacttttt tggttaaaat aagagcatcc gtaaccgcaa
     1081 ttaaaggtgg agaatccaca agaacaaagt cataacggga tttaagttcc tccaacagaa
     1141 aatcagttct tggagaagca atta
//
LOCUS       DAOPFL010000259         1112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_196210_length_1112_cov_52.881741, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000259 DAOPFL010000000
VERSION     DAOPFL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196210_length_1112_cov_52.881741"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLG69_08485"
     CDS             complement(<1..655)
                     /locus_tag="PLG69_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPI43577.1"
                     /translation="MRQVVVVCAKRSPIGNFGCVFKDISAINLGVTVLQSMLKETGLK
                     PELIDEVIIGNVCGAGLGQNVARQIAIHSGIPETVPAYTVNKVCGSGMKAVSLASLMI
                     GSGEADIIVAGGTENMSQIPYALTDARWGARMGNKEMVDLMIRDGLSDIFNNYHMGIT
                     AENLAEKYHISREEQDAFAAQSQNRTEEAQKAGRFKDEIVPFSIPQRKGEPIIVDKDE
                     "
BASE COUNT          326 a          239 c          165 g          382 t
ORIGIN      
        1 tttcatcttt atctacaatt atcggctctc cctttctttg gggaatgctg aaagggacaa
       61 tttcgtcctt aaatctaccc gctttttgtg cttcttcagt tctattctga ctttgggctg
      121 caaaagcatc ctgttcttct ctgctaatat ggtatttttc tgccaaattt tctgctgtaa
      181 tacccatatg atagttattg aaaatatcgc taagcccatc ccgaatcatt aaatccacca
      241 tttccttatt acccattctg gctccccaac gcgcatctgt taaggcataa ggtatctgac
      301 tcatattttc tgttccaccg gctacaataa tatctgcctc tccggaacca atcataaggg
      361 aagctaaaga aaccgctttc attcctgaac cgcaaacctt atttacagta taagcaggaa
      421 cagtttcagg aatgccactg tgaatggcta tctgcctggc tacattctgc cctaaaccag
      481 ctccgcaaac attgccgatt atcacttcat caattagttc cggctttaat cccgtctcct
      541 tcaacattga ctgtaaaacg gttactccta aattaatcgc ggaaatatct ttgaaaacac
      601 atccgaaatt accgatagga ctgcgtttgg cacataccac tacgacctgt ctcattttgt
      661 ttctcctgta ttgttaattt tcttattact acattgcaaa aaaagtgccc cttttcgtca
      721 acaaaaatga tgtagcacgc agattacgct gataacgcag attttatgat ttactaatat
      781 ataacatcct gcagatccag tcgatccagt tatttgcgtt ctaataacct ttattcttcc
      841 ttcttcattt ttttctcttt cctctttctt tgtgaaaata aaaaaatttt agggggggtt
      901 gtggaaaaat atcaacagta atatgagggt taacatcttt agattgaata tattagcaat
      961 tatacctaat ctatatctca taaatttaaa aaaaaatacc cctgagaggg attgacataa
     1021 atacggggat aaagaataag gcaatggcaa atcaataaat atgtttattt gtttcataat
     1081 tgtttgattt attattttaa atctttataa gc
//
LOCUS       DAOPFL010000260         1106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_197923_length_1106_cov_124.577545, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000260 DAOPFL010000000
VERSION     DAOPFL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197923_length_1106_cov_124.577545"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLG69_08490"
     CDS             complement(<1..1038)
                     /locus_tag="PLG69_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43578.1"
                     /translation="MQKYQIVIILITALLFSACCHHKTKEVTPETENFNYTGSKFHNN
                     YIWGGAMNLAWNELIESFTSEPIELDTKDESTLKTLYCLNNPVFSKADIDEASYYVRS
                     GYGQKTVNLINSECKAKFPDKTIPNLQLKLGDRDIISYAYFLKQIEYAIAFQKQELNF
                     MDQKVNGFTSGAESYSNVYILAYENDDKFLLALKLKDNQDQIFLAKGFPMDKPDEVLK
                     MLKTKAPAQEIADFMPGTVMNRKDIFQAPILHLNYERQYKEMLNKKVTNKKLDGYQIA
                     LMQEIIKFDMDEKGARVENEAIIGMITSINPDAYKPKNLILDKPYWVIMKRANSNNPY
                     FILGVGDTKLMQ"
BASE COUNT          323 a          192 c          186 g          405 t
ORIGIN      
        1 ttgcatcaat tttgtatcac caactcccag aataaaatag ggattattgg aattagcccg
       61 tttcataatc acccaatagg gcttatctaa gatcagattt ttgggtttat aagcatctgg
      121 atttatggaa gttatcattc cgatgatagc ttcattttca acgcgggcac ctttttcgtc
      181 catatcaaat ttgataattt cctgcatcag agcaatttgg taaccgtcaa gttttttgtt
      241 tgtaaccttt ttatttagca tttctttata ctgtcgttca taatttaggt gtaaaatcgg
      301 tgcctgaaaa atatctttcc ggttcattac agttccgggc ataaaatctg ctatttcttg
      361 agctggtgcc tttgttttca gcatttttaa gacctcatcg ggtttatcca ttggaaatcc
      421 ctttgccaga aaaatctggt cttggttatc ttttaatttc aaagcgagca agaacttatc
      481 gtcattttca taagccaaaa tataaacatt agaatagctt tcagcaccgg aagtaaaacc
      541 attaactttt tgatccataa aattcaattc ctgcttttgg aaagcgattg catattcaat
      601 ttgtttaaga aaataggcat aagagataat atctctgtca cctaatttta actgcaaatt
      661 tggtatagtt ttgtcaggaa attttgcctt gcattcagaa ttgataagat taactgtttt
      721 ttgaccgtat ccacttctca cataataact tgcctcatct atatcggctt tagaaaaaac
      781 gggattattt aagcaataca atgttttaag tgtggattca tctttagtat caagttcaat
      841 gggttcactt gtaaaacttt ctatcagttc attccaggca agattcattg ctccgcccca
      901 aatataattg ttgtgaaatt tactgccggt ataattaaag ttttcagttt ccggagtgac
      961 ctctttggtt ttatggtggc agcaagccga aaataagagg gcagttatca ggattatgac
     1021 aatttgatat ttctgcattt tttaacctca tttcatcttt ttggcaggat aaattacggg
     1081 gaaattgtgt caataacaat gtatat
//
LOCUS       DAOPFL010000261         1094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_201393_length_1094_cov_102.782483, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000261 DAOPFL010000000
VERSION     DAOPFL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201393_length_1094_cov_102.782483"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..>1094)
                     /locus_tag="PLG69_08495"
     CDS             complement(340..>1094)
                     /locus_tag="PLG69_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPI43579.1"
                     /translation="PASYCGVTGLKPSYGLFSLQGVFPFAQTVDHLGFITKRVADLAF
                     LLPLLHLSVSAKITDRPVLGAVNGSYLQQADYSVQENYCSALDKLQKKGYKIVTCDPF
                     GDINSINASHNALIAAEFSLNHKSLYERYQELYSDSSKELYARGLQISAAELENLRQQ
                     TIILREKIINLMQEQKIDLWITPSTSTTAPAGLKSTGSPLMSLPWTNCGLPALTIPNG
                     LGANGLPFGLQFVASYNEDLPLVRMVQRIVNT"
BASE COUNT          337 a          217 c          209 g          331 t
ORIGIN      
        1 agagagccat tatctctttt ccctggaatg agttccgtag gaacgacaga tattatagaa
       61 gggttttaac cctgagcaaa aaaatcaaat tttgcatttg atttgctaaa ttctaatttg
      121 ccataaatag aagttattta tttttcagtg ttcttataac tctttggcaa attggaagtt
      181 gccaacctat tagccttcaa ttgcattatt cggtagagaa aattgtccat catttttcag
      241 agttccgcaa actctttggc aaatcggaat ttatcacccc cttttagttc aattgcatta
      301 ttcgggtgca aaatcctgca acctttaagt ccctatttcc tatgtattaa caatcctttg
      361 aaccatccta accaatggca gatcttcatt ataggaagcc acaaactgca agccgaaagg
      421 aagaccattg gcgcctaagc cattgggaat agttaaagct ggcaaaccgc aattcgtcca
      481 gggcaaactc attaaaggtg aaccggttga ctttaatccc gcaggagcag tagttgaggt
      541 agaaggagta atccagaggt caattttttg ttcttgcatc agatttatta ttttttctct
      601 aagtataatt gtctgttggc gtagattttc cagttccgcg gcactgattt gtaatcctct
      661 ggcatacaat tccttagagg aatcggaata aagttcctga tagcgctcat ataaactttt
      721 atgatttaag gagaattctg ccgcaatcag ggcattatgg ctcgcattaa tagagtttat
      781 atcgccaaaa ggatcgcagg taactatttt gtatcccttt ttctgcagtt tatcaagagc
      841 actgcaataa ttttcctgaa cggaataatc cgcctgttgt aaataacttc cgttaacagc
      901 acccaaaaca ggtctgtctg ttattttggc agaaacggac agatgcaaaa gaggcagtaa
      961 aaaagcaagg tctgctactc gttttgtgat gaaaccgaga tgatcaactg tttgagcaaa
     1021 aggaaaaacc ccttgcagag aaaacaaacc ataactaggt tttaaacctg taactccaca
     1081 gtaagaagca ggcc
//
LOCUS       DAOPFL010000262         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_205724_length_1080_cov_62.228293, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000262 DAOPFL010000000
VERSION     DAOPFL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205724_length_1080_cov_62.228293"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLG69_08500"
     CDS             complement(<1..740)
                     /locus_tag="PLG69_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43580.1"
                     /translation="MAKRKFVILLFIIAAVLVGAQTIPYPVDSKEYQDYKEGLIGKMP
                     IQPPVYSNVPKGKQIPLVFQRQQREGLLIPLDDSFQLAMEGNDDYYTSVIQLPFAFDF
                     YGTTYTEFYINNNGNVSFGNPYSTYTSYGFPVNGFPMLAPFWADVDTRSGGQVFYKME
                     SNRVTVIWNAVGYFSNHTDKLNTFELVFTNGWDPVVGLGYNVAFSYGDMQWTTGDASG
                     GQNGFGGVPATVGLNKGDGIYYGLIGRF"
     gene            complement(742..>1080)
                     /locus_tag="PLG69_08505"
     CDS             complement(742..>1080)
                     /locus_tag="PLG69_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPI43581.1"
                     /translation="MWSFTTGNYGAGNISMLEPVPVTLAINALQNQVRLSWQRRRDEE
                     SYYVYWSENPYAEFPEGWNGPVNVTETSFTDTLSDKCFYRVFAVPNMRGEIENKQNTK
                     PRAIMPKRGE"
BASE COUNT          325 a          258 c          183 g          314 t
ORIGIN      
        1 tcaaatctgc cgattaaacc atagtaaatt ccatctcctt tgtttaaacc aaccgttgcc
       61 ggaactcctc caaaaccatt ttgacctccg gaagcatctc cagttgtcca ttgcatatct
      121 ccataactga aagcaacatt ataacccaac cccacaacag ggtcccaacc attagtaaaa
      181 acaagttcaa aggtattcag tttatcggta tgattagaaa aatagccaac ggcattccag
      241 attaccgtta ctctgttgga ttccattttg taaaagacct gaccacctga ccttgtatct
      301 acatccgccc aaaagggagc cagcatcgga aatccgttta ctggaaatcc ataagaagtg
      361 taggtactgt aaggattgcc aaaagaaaca ttcccgttgt tgttaatgta aaattccgtg
      421 taagtagttc cataaaaatc aaaagcaaac ggcaattgaa tcacggaagt gtaataatca
      481 tcatttcctt ccattgccag ctggaaacta tcatccaacg gaattaaaag accctccctc
      541 tgttgtcttt gaaacaccag aggaatctgt ttcccttttg gcacattgga atataccggt
      601 ggctgaatag gcattttacc tatcaaaccc tctttgtagt cctgatattc cttggagtcc
      661 acaggatagg gaattgtttg ggcgccaact aatactgctg caattataaa cagcagaatt
      721 acaaactttc tttttgccat tttattctcc tcttttaggc ataattgccc tcggctttgt
      781 attttgttta ttttctattt ctccccgcat attaggcacg gcaaataccc ggtagaaaca
      841 tttatcggag agagtatccg taaagctggt ctcagttaca tttacaggac cgttccaacc
      901 ctcaggaaac tccgcataag gattttccga ccaatataca tagtagcttt cctcatccct
      961 tctgcgttgc caggataaac gcacttgatt ttgtaaggca ttaatcgcta aagtaacagg
     1021 aaccggttct aacatagata tattacccgc tccataatta ccggtagtga aactccacat
//
LOCUS       DAOPFL010000263         1065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_210365_length_1065_cov_77.372277, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000263 DAOPFL010000000
VERSION     DAOPFL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210365_length_1065_cov_77.372277"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..>1065)
                     /locus_tag="PLG69_08510"
     CDS             complement(91..>1065)
                     /locus_tag="PLG69_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPI43582.1"
                     /translation="TQFSENTLTELRAALDNLEKQNVKGLIIDLRWNPGGLLEQAVDT
                     VNEFIGKNRLVVETKGRTENKQLYTRFDVKERNYPIIVLVNEASASASEIFAGSLQDW
                     DKGLVMGKNTFGKGSVQQLIPLSNGNGIKITTAYYYIKSGRCIHKMSNDNILKGKEVS
                     EEDLKKESETNLQQVYYTANNRKVYGGGGIQPDITVESDFLTLFGVELRRKNVFFNFA
                     VDYLVQHNHQFDINTEIDNKIMNEFLTYVSKNDIKYTQADVDSANSFIRTSIKSELVR
                     KVHGDEEAYKITISQDKQLMEAISLFDRFRTLEQMFTYAASLNEKKEK"
BASE COUNT          284 a          211 c          188 g          382 t
ORIGIN      
        1 ttggttcaat tacctcaggt aatgaacaat aatatttttt ttcttaaggt attgtgttat
       61 ctgaactacc ctattggttg aacacactcc ctatttttct tttttctcgt ttagggaagc
      121 ggcgtatgta aacatttgtt ctaaggttct gaaacgatca aacagagaaa ttgcttccat
      181 caactgttta tcctgagaaa tagttatttt atatgcttct tcgtcaccat gcactttgcg
      241 gacgagttca cttttaatag aagtgcggat aaaactattg gcactatcta catctgcctg
      301 agtgtattta atatcatttt ttgaaacata agtcaggaat tcgttcataa ttttgttatc
      361 aatttcggta ttgatatcaa actggtggtt atgttggact aaatagtcca cggcgaaatt
      421 gaagaaaaca tttttgcggc gtaattcaac tccgaaaagg gtgagaaaat cgctttctac
      481 agtaatatca ggttgaattc ctcctcctcc atagacctta cggttattgg cagtataata
      541 gacctgttgg aggttggttt cgctttcttt tttcaggtct tcttcgctta cttctttgcc
      601 tttcaggatg ttatcattgc tcattttatg aatgcagcgt ccgcttttga tgtaataata
      661 ggcagtagta attttaattc cgttgccgtt ggaaagagga ataagctgct gcacactacc
      721 tttgccgaag gtatttttgc ccataactaa gcccttatcc caatcctgta aactgccagc
      781 aaaaatttcc gatgcggatg ccgatgcttc attcacaagc acgataatag gataatttct
      841 ttctttaaca tcaaatctgg tatagagttg cttattttcc gttctacctt tggtttccac
      901 tactaaacgg tttttcccga taaattcatt aactgtatcc actgcctgtt ctaaaagtcc
      961 tcccggattc caacgcaggt ctataatcaa gcccttaaca ttttgttttt ccaggttatc
     1021 aagagcagcc cttagctcgg ttaaagtatt ttcactaaat tgagt
//
LOCUS       DAOPFL010000264         1042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_218003_length_1042_cov_79.072948, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000264 DAOPFL010000000
VERSION     DAOPFL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218003_length_1042_cov_79.072948"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLG69_08515"
     CDS             <1..456
                     /locus_tag="PLG69_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPI43583.1"
                     /translation="GFAMRIIYYNDVPVSNTLPFETTETDLQTLLKEADIITLHLPLT
                     KETFHLIGKEEFALMKENAVLINTSRGAVIDEKELIKALSEKRIFSAGLDVYENEPDI
                     PQELLALENVVLLPHIGSASIETRTKMALLAAENAIAVMEGRKPPAQVN"
     gene            634..>1042
                     /locus_tag="PLG69_08520"
     CDS             634..>1042
                     /locus_tag="PLG69_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPI43584.1"
                     /translation="MQKTNIGIFSGLSEEVVEELTKDLERIKFKKGEAIITEHSLSDN
                     LFFISKGKVEIDKDLSNPDIPFTQLSTLGAGDFFGEMGIIEDSTRSASVVATEDVELM
                     VIPREAFSEMLFAYPIIMLNLTKVISSRLRNTNE"
BASE COUNT          354 a          177 c          221 g          290 t
ORIGIN      
        1 ggttttgcaa tgcggattat ctattataat gatgttcccg tttccaatac tttacctttt
       61 gaaacaacgg aaacggattt gcaaacactg ctgaaagaag cggatattat taccctgcac
      121 ctgcccttaa caaaagagac atttcacctt ataggcaaag aggaatttgc cttaatgaaa
      181 gaaaatgccg tgctcataaa tacttccaga ggagcagtta ttgatgaaaa agaactgatt
      241 aaagcgcttt ctgaaaaacg cattttttcc gcaggactgg atgtttacga aaatgaaccc
      301 gatattcctc aggaactttt ggctttggaa aatgtggttt tactacctca tatcggctct
      361 gccagcattg aaaccagaac caaaatggca cttttggcgg cagaaaatgc cattgccgtt
      421 atggaaggaa gaaaaccacc ggctcaagta aattaagtaa cacggaattg actaactacc
      481 cggattgaca taaattgcat atttatatcg ggacttgcaa gagaaaaatt ataaaaaatg
      541 attaaaactt gacaaaaaag ggagggtgat ttattatata atatataagg ttaataaagt
      601 tagaagatgt gaggtatata tatgcaggaa acaatgcaaa agaccaatat agggatattc
      661 agcggcctct ctgaagaagt agtggaagaa ctgactaaag atttggagcg gattaaattt
      721 aagaaaggag aggcaataat cactgaacat tccctcagcg ataatctctt tttcatctct
      781 aagggtaagg tggaaataga caaggactta agcaatcccg acataccttt cactcagctc
      841 tcaaccttag gggcgggaga tttttttggc gagatgggca ttattgaaga tagtactcgt
      901 tctgccagtg ttgtagctac agaagatgta gagttaatgg ttattccccg ggaggcattt
      961 agtgagatgc tctttgctta tccgataata atgttgaatt taactaaggt aatttccagt
     1021 cgcttgcgta atactaatga aa
//
LOCUS       DAOPFL010000265         1032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas acidaminovorans isolate
            AMR_MDS_3382 NODE_221438_length_1032_cov_108.333675, whole genome
            shotgun sequence.
ACCESSION   DAOPFL010000265 DAOPFL010000000
VERSION     DAOPFL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865117
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas acidaminovorans (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas acidaminovorans
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/02/2023 19:33:56
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,705
            CDSs (total)                 :: 1,665
            Genes (coding)               :: 1,665
            CDSs (with protein)          :: 1,665
            Genes (RNA)                  :: 40
            tRNAs                        :: 36
            ncRNAs                       :: 4
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Cloacimonas acidaminovorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221438_length_1032_cov_108.333675"
                     /isolate="AMR_MDS_3382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:456827"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..>1032
                     /locus_tag="PLG69_08525"
     CDS             32..>1032
                     /locus_tag="PLG69_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPI43585.1"
                     /translation="MNTPKRVYLFGNGKAEGSAEMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTEVCTEYNKLGSAQLREILSDDVHNAIRHIEDLVNMQFGSAENPLLVSVRSGA
                     RASMPGMMDTILNLGLNETTIQGLIKKTNNERFAWDSYRRFVQMYSDVVLGLKPESKE
                     EHDPFEVIIDTVKKEKGISSDLELTTEDLKNLVTIFKQTVEEKTGKKFPDDPWEQLWG
                     AIFAVFDSWNNERAKFYRKLNNIPDEWGTAVTVQAMVFGNMGFTSATGVAFTRDAATG
                     ENLFNGEYLINAQGEDVVAGIRTPQQITKIGSQRWAKLAGISEEERLSKYPSLEEAMP
                     "
BASE COUNT          321 a          184 c          237 g          290 t
ORIGIN      
        1 tctttaatga tgttaaagtt atggaggata aatgaataca cctaaaagag tttacctctt
       61 cggaaatgga aaagccgaag gtagcgcaga aatgaaaaat cttctcggtg gaaagggtgc
      121 caaccttgcc gagatgaatc ttattggcgt tccggttcct cccggattta caattactac
      181 agaggtctgc actgaataca acaaattggg ttctgcccaa ttgagggaaa ttttaagtga
      241 cgatgtgcac aatgccattc ggcatataga agaccttgta aatatgcaat ttggctctgc
      301 cgaaaatcca cttttggttt cagtgcgttc aggtgctcgt gcttcaatgc ctggaatgat
      361 ggataccatt ttgaatttgg gcttgaatga aacaactatt cagggtttaa tcaaaaaaac
      421 caataatgaa aggtttgcct gggatagtta tcgtcgtttt gtccaaatgt attctgatgt
      481 ggttttaggt cttaaaccag aaagcaaaga agaacatgat ccctttgaag ttattattga
      541 caccgtgaaa aaagaaaaag gcatcagcag cgatttggaa ctaaccactg aagatttgaa
      601 aaatctggta accatcttca agcagacggt ggaagaaaaa accggtaaaa aatttccgga
      661 tgacccctgg gaacagctct ggggtgcaat ttttgctgtt tttgatagct ggaataatga
      721 acgcgcaaag ttttatcgta aactgaataa cattcccgat gaatggggaa ctgcagtaac
      781 cgttcaagca atggtttttg gcaatatggg ctttaccagt gctactggtg ttgcttttac
      841 tcgtgatgct gctaccggtg aaaacctttt taatggagag tatttgatca atgctcaagg
      901 tgaagatgtt gttgccggaa tcagaactcc tcagcaaatc acaaaaattg gttctcagcg
      961 ttgggcaaaa cttgccggta tcagtgaaga agaacgctta agtaaatatc cttctctgga
     1021 agaagcaatg cc
//
